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Revenue Cycle Management
U.S. healthcare changed considerably over the last decade, transitioning into a more technologically advanced and regulated industry. Some of the most notable drivers include increasingly more stringent regulations like President Obama's Patient Protection and Affordable Care Act (ACA), more detailed coding systems, and evolving valued-based reimbursement and payment models.
Although these changes present challenges and increased costs for healthcare providers, they also create sizeable business opportunities for the outsourced business services (OBS) sector, particularly companies specializing in revenue cycle management (RCM). RCM is an integral outsourced service for healthcare providers because of changes in legislation and regulation, administrative operations, and consumer demographics.
Overall, the RCM industry is a relatively young segment of the OBS sector that developed over the past few decades as a result of the evolving, and expanding, U.S. healthcare market. Before that, healthcare providers were primarily small-town physicians who treated neighbors and friends for unstructured costs, and were sometimes only paid if patients recovered. Between the 1980s and 1990s, large IT management companies began deploying systems designed to help clients more effectively manage their transaction processing, billing and receivables management needs as the RCM industry slowly gained recognition and traction within the healthcare industry.
The RCM industry changed drastically since its inception when it only handled billing and collections, and basic administrative functions. Today, healthcare is a technologically advanced market that requires expertise in treating medical problems and handling administrative functions through sophisticated billing and payment processing networks, and electronic health record systems.
In 2015, the RCM industry produced about $18 billion in revenue, and is projected to grow to more than $26 billion in annual revenue by 2021 at a compounded annual growth rate of 5.5 percent.
As a result of the ongoing evolution of the healthcare industry, the RCM industry continues to grow and attract interest from financial and strategic buyers. Kaulkin Ginsberg has worked with a number of these companies over the years, serving as both a strategic and transaction advisor to board-level executives.
We understand the history of the RCM industry, continue to garner expertise within it, and have the foresight to know where the industry is heading. We are excited about the potential within the RCM industry and look forward to working with owners and executives who are committed to achieving their growth and exit objectives.
If you are interested in receiving a copy of The Healthcare Revenue Cycle Management Industry Overview, or if you would like to confidentially discuss your interests in the RCM industry, please contact a member of our strategic advisory team at email@example.com.
Possible H-1B Visa Reform on the Horizon will Impact the ARM Industry
February 24, 2017
Human capital is a crucial aspect of efficient and innovative business processes, as well as a country's long-term growth potential. As such, the implications associated with changing the existing H-1B visa program structure will have far reaching consequences on all industries, including the outsourced business services. ....» see this post » all posts
Establishing Value Before Going to Market
February 1, 2017
Kaulkin Ginsberg recommends business owners establish a realistic value for their business before going to market. This ensures we are on the same page with our clients, and avoids causing undo stress to the management team by starting and stopping a sale process....» see this post » all posts
Check Payments: A Dying but Meaningful Market
January 19, 2017
Before the 21st century, collecting bad checks was one of the largest sources of new business for debt collection agencies. Innovations in fintech are continually changing consumers' transaction modes through the use of advanced technologies like mobile payment systems, and checks are becoming increasing rarer as a means of noncash payments. We examine trends within checking and discuss how changes in this market will affect the ARM industry. ....» see this post » all posts
Mike Ginsberg Leading Panel Discussion at DBA International 20th Anniversary Conference
February 1, 2017
Mike Ginsberg, President and CEO of Kaulkin Ginsberg, will be speaking in a panel discussion on Trends in Debt Buying on Wednesday, February 8th at the DBA International 20th Anniversary Conference in Las Vegas, Nevada....
Spring 2017 Research Fellowship Program Analyzes International Outsourced Business Services
February 1, 2017
Kaulkin Ginsberg, the leading consultancy and M&A advisory firm for the outsourced business services (OBS) industry, began the eighth semester of its Research Fellows Program on January 30th in conjunction with the University of Maryland, College Park's Department of Economics. Fellows will be analyzing and developing market research reports on the German, U.K., Australian, and Canadian OBS industries, with a particular focus on collection agencies and call centers.....
Kaulkin Ginsberg Releases the Healthcare RCM Overview
November 1, 2016
This report provides analyses of the current and anticipated challenges facing the healthcare market, trends facilitating growth, and an outlook for the future.....


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Republican leaders in the House of Representatives on Dec. 22 announced that they would accept compromise legislation that provides a two-month extension of the payroll tax cut set to expire at the beginning of the year.
For several days, House leaders had balked at a short-term, bipartisan compromise which Senate leaders had developed to give both chambers time to work out terms of a year-long extension of the cut. Under pressure to extend the cut, House leaders yesterday said they would accept the Senate's agreement, with the addition of a new provision.
According to a statement issued yesterday by House Speaker John Boehner (R-Ohio), "a new bill will be approved by the House that reflects the bipartisan agreement in the Senate along with new language that allows job creators to process and withhold payroll taxation under the same accounting structure that is currently in place."
The "bipartisan agreement in the Senate" cited by Boehner does not include any of the federal workforce provisions contained the Middle Class Tax Relief and Job Creation Act (H.R. 3630), which the House approved earlier this month. That bill, which would extend the payroll tax cut for a full year, also would extend the current two-year federal civilian pay freeze for an additional year, and make changes to federal retirement benefits.
Congressional leaders said they would ask members of the House and Senate to approve the legislation by unanimous consent, a procedure which would permit passage without calling lawmakers into session for a formal vote.
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Save the date for 28th annual Federal 100 Awards Gala.
March 30, 2017
The Department of Commerce is revising its confidentiality agreement for statistical data survey respondents to reflect the fact that the Department of Homeland Security could see some of that data if it is captured by the Einstein system.
In a Feb. 17 memo, Defense Secretary Jim Mattis told senior leadership to establish teams to look for duplication across the armed services in business operations, including in IT and cybersecurity.
State election officials had more questions than answers after a Department of Homeland Security presentation on the designation of election systems as critical U.S. infrastructure.
The government desperately needs younger talent to replace an aging workforce, and experts say that a freeze on hiring doesn't help.
The Department of Veterans Affairs will test an internally developed scheduling module at primary care sites nationwide to see if it's ready to service the entire agency.
After missing in the last Congress, drafters of a bill to encourage cloud adoption are looking for a new plan.
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Microdermabrasion & Oxygen Facial£39 instead of £100 for a microdermabrasion treatment and oxygen facial at Park Private Clinic, Nottingham - save 61%
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- Enjoy a microdermabrasion treatment and oxygen facial.
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The Fine Print
- One per person.
- May buy multiples as gifts.
- Voucher valid until 2nd May 2014.
- Cannot be used in conjunction with any other offer.
- Subject to availability.
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Our skin tells the story of our life, but while memories may be fond that doesn't mean you want them splashed over your face! Regain some mystique with today's deal...
We're offering you a microdermabrasion treatment and oxygen facial for just £39, saving you 61% off Park Private Clinic's price of £100.
Based in Nottingham city centre, the highly-regarded Park Private Clinic is home to a talented team of therapists with the aim of making you feel better and look fabulous. You'll enjoy a 75-minute treatment consisting of one microdermabrasion session and a Crystal Clear oxygen facial. When you arrive you'll get a skin analysis to set out the required microdermabrasion. Your skin will then be cleansed and oxygen cream will be applied for 20 minutes of oxygen therapy. Finally, brightening and repair creams are applied before it's all finished off with a revitalising toner. Sounds good to us!
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Articles in Mansfield MA
Fleet Vehicle Maintenance Mansfield MA
Your Mansfield fleet service maintenance resource for fleet businesses or vehicle owners. Find the local information and resources you need in Mansfield, MA. Whether you are looking for fleet vehicles, it maintenance services, auto parts, mechanics, car parts, oil change services, car leases, auto shops, or repair shops, or have questions about fleet services, this page will help you get the information you are searching for.


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Auto Clinic Care
Auto Repair Shop, Vehicle Repair, Auto Repair Services
There is no doubt about it, we gladly perform automotive engine repairs. That is partly why we are known as the Auto Clinic. No matter what the make or model of your vehicle is, we will service your engine. Whether you have small engine problems or major engine problems that may require replacement, we will take the job. Of course we do automotive engine repair! That's why we're Auto Clinic Care for Rockville, MD and the surrounding area. Whatever the make or model - From minor fixes to engine rebuilds and replacement, we provide ASE-professional, top quality service at affordable prices. We're renowned in the area for our work and exemplary service. Customers continually recommend us as the place to go for any car repair needs.
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If you are interested in services that you may not see listed on our website, feel free to give us a call.
You can bring your car, truck, or van to us for complete transmission and brake repair.
Auto Clinic Care provides a one stop shop for your vehicle inspection and repair needs.
Let us help you avoid excessive repairs to your vehicle by doing the necessary and timely maintenance checks.
We want to help you to maximize the performance and life of your vehicle with simple preventative maintenance services.
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The Metra board of directors has selected Alexander D. Clifford, an executive with the Los Angeles County Metropolitan Transportation Authority, to be the commuter rail agency's executive director and chief executive officer.The appointment was made Tuesday in a unanimous vote by the 11-member Metra board after a nationwide search conducted by Slavin Management Consultants, a government executive search firm."His experience as a big-picture policy-maker and a day-to-day operations manager make him uniquely qualified to lead Metra," Metra Chairman Carole R. Doris said. "It is my belief that he is the right man for the job at the right time."Clifford said he is coming to Metra with a reform agenda. "The Metra Board has tasked me with restoring public confidence in our agency by instilling strong financial and management controls and inspiring Metra employees to meeting these important challenges," he said. "We will also continue to modernize our operation including incorporating new technology for better customer communications."Clifford, 51, most recently served as Executive Officer, High-Speed Rail, for Metro, working as an ombudsman with the California High Speed Rail Authority to help facilitate the integration of high-speed train service into existing transportation corridors in Los Angeles County. He was responsible for making sure the interests of the county, various municipalities and other stakeholders were represented and that the potential impacts of the project were properly addressed.Clifford also oversaw Metro's interest in the Southern California Regional Rail Authority (Metrolink commuter rail) as well as its interests in the Los Angeles-San Diego Rail Corridor Agency (LOSSAN).From February 2004 to August 2009, Clifford oversaw all aspects of bus service in 26 cities in southeast Los Angeles County as the general manager of the Gateway Cities Service Sector, one of five Metro service sectors. The sector, with an annual budget of $128 million, operated two bus divisions with a fleet of 457 buses and 1,040 employees. Clifford worked with a nine-member governance council composed of elected officials and citizen transit members that were responsible for bus service in the sector.As managing director, operations, from January 2003 to February 2004, Clifford managed the bus operation control center, the bus regional rebuild center and served as the executive lead for the installation of a Universal Fare System and the Advance Transportation Management System on all Metro buses. He was on the 2003 and 2008 transit community policing contract negotiations team. Clifford also served as the executive administration manager (April 2002 to December 2002), where he managed various performance and budgetary functions, and as a senior administrative analyst (July 2001 to March 2002).Clifford has a Master of Business Administration degree from California Baptist University and a Bachelor of Science degree in Business Administration from California State Polytechnic University.
The incident occurred at 2 a.m., when the driver pulled up to the last stop of his shift and asked the man to leave the bus. After a physical altercation, which ended up outside the bus, ensued, the driver was stabbed multiple times.
Twenty-eight 40-foot buses will replace existing buses in the NICE fleet that have reached the end of their useful life, while five 60-foot buses will be used on the customer's new bus rapid transit line, scheduled to start service in 2017.
The ABB DC fast charger, with the associated systems, is based on the common interface, which allows charging stations and electrified buses from different manufacturers to be used together.
Will lead the end-to-end customer lifecycle as Proterra triples production and initiates large-scale deployments across North America.
The battery-operated jack can be charged overnight and used without cables. The pneumatic jack has an attached air hose to keep it working. Previously, transmission jacks were manually hand-pumped, much like a car jack.
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Operations and Maintenance is a vital aspect of owning a solar water heater. Take care of your system with these tips from NIBS.
Photo Credit: SKYLINE INNOVATIONS
Just three years ago, solar water heating growth in commercial buildings was poised to grow by 20% per year, according to a report from research and consulting firm AltaTerra Research. Even the White House has joined the craze by utilizing a rooftop solar water heater – but have you?
If so, you should abide by some basic operations and maintenance principles, recommends Andy Walker, principal engineer at NREL and author of Solar Water Heating for the National Institute of Buildings Sciences' Whole Building Design Guide.
O&M for your system is similar to that required of any hydronic heating loop and may be provided by site staff. Experts can be called in if anything fails, he adds.
Regularly scheduled maintenance should include:
Additional maintenance can include restoring temperature sensors that have been disconnected, replacing pump capacitors and motors, repairing leaks or damage from freezing, and fixing glass broken by vandalism or environmental factors, the report reads.
At some point – typically in excess of 10 years – the storage tank may need to be replaced, it adds. But after enjoying 10 years of free hot water, that cost is just a tall glass of lemonade.
Polyglass U.S.A., Inc. has been manufacturing premium roofing products in North America for 25 years. Learn about our CURE Technology and ADESO Technology, innovations that have transformed the roofing industry.
Utility Vehicle Alert! Don't Get Framed. Rust can eat right through most UTV frames, destroy your investment and disable your crews. But this simple step stops rust in its tracks.


text: June 8, 2010 | perivision | 1 Comment Oh lets hope so. Its total rumor, but I just read on androidandme.com that t-mobile will be updating 3G myTouch phones. That COULD also mean the Google Ion I/O phones we got in 2009, aka, the Magic. For those of you who still have your Google Ions, it has been stuck at 1.6 forever just like the myTouch. I have been trying to find a way to upgrade it with little luck. However with this new announcement, we could get 2.2 and hope over hope, multi-sense??? So although the myTouch is official, there is not guarantee, that the Ion phones will be updated even though we saw on running at the I/O show. I did not see it in person, so I do not know how well it ran on the weak processor, but from what I'm told, the new OS is suppose to be faster then the 1.6 we have now, so again, that could be a full win for HTC touch/magic users. I'll keep you all posted as I hear more or find my HTC magically updated. 🙂 Follow


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Tryfonidis K.,European Organization for Research and Treatment of Cancer EORTC Headquarters |
Zardavas D.,Breast International Group Headquarters BIG aisbl |
Cardoso F.,Breast Unit
Cancer Treatment Reviews | Year: 2014
Small (T1a, b), lymph node negative breast tumors represent an entity diagnosed with increasing frequency due to the implementation of wide-scale screening programs. Patients bearing such tumors usually exhibit favorable long-term outcomes, with low breast cancer mortality rates at 10. years, even in the absence of adjuvant chemotherapy. However, most available data derive from retrospective studies. Additionally, a subset of patients with these tumors experience recurrence of the disease, indicating that early tumor stage itself is not a sufficient prognosticator. It is of paramount importance to refine the prognosis of this population, identifying patients with high risk of recurrence, for whom adjuvant treatment is needed. The underlying biology of the disease provides relevant information, such as grade and status of hormone receptors and HER-2 (human epidermal growth factor receptor 2), with high grade, triple negative and HER-2-positive tumors having worse prognosis. Additionally, multigene signatures may improve further the prognostication of patients with small, node negative breast cancers. Further research for this increasingly frequent group of patients is urgently needed, so that better informed clinical decision making, in particular regarding adjuvant chemotherapy, can occur. © 2014 Elsevier Ltd. Source
Clinical application of circulating tumor cells in breast cancer: Overview of the current interventional trials
Bidard F.-C.,University Pierre and Marie Curie |
Bidard F.-C.,University of Paris Descartes |
Fehm T.,University of Tubingen |
Ignatiadis M.,Institute Jules Bordet |
And 10 more authors.
Cancer and Metastasis Reviews | Year: 2013
In 2004, circulating tumor cells (CTC) enumeration by the CellSearch® technique at baseline and during treatment was reported to be associated with prognosis in metastatic breast cancer patients. In 2008, the first evidence of the impact of CTC detection by this technique on survival of cM0(i+) patients were reported. These findings were confirmed by other non-interventional studies, whereas CTC were also investigated as a surrogate for tumor biology, mainly for HER2 expression/amplification. The aim of this report is to present the current prospective large interventional studies that have been specifically designed to demonstrate that CTC enumeration/characterization may improve the management of breast cancer patients: STIC CTC METABREAST (France) and Endocrine Therapy Index (USA) assess the CTC-guided hormone therapy vs chemotherapy decision in M1 patients; SWOG0500 (USA) and CirCe01 (France) assess the CTC count changes during treatment in metastatic patients; DETECT III (M1 patients, Germany) and Treat CTC (cM0(i+) patients, European Organization for Research and Treatment of Cancer/Breast International Group) assess the use of anti-HER2 treatments in HER2-negative breast cancer patients selected on the basis of CTC detection/characterization. These trials have different designs in various patient populations but are expected to be the pivotal trials for CTC implementation in the routine management of breast cancer patients. © 2012 The Author(s). Source
Efficacy and safety of bexarotene combined with psoralen-ultraviolet A (PUVA) compared with PUVA treatment alone in stage IB-IIA mycosis fungoides: Final results from the EORTC Cutaneous Lymphoma Task Force phase III randomized clinical trial (NCT00056056)
Whittaker S.,Kings College London |
Ortiz P.,Hospital Universitario 12 Of Octubre |
Dummer R.,University of Zurich |
Ranki A.,University of Helsinki |
And 6 more authors.
British Journal of Dermatology | Year: 2012
Background Psoralen plus ultraviolet A (PUVA) is the standard treatment for early stages of mycosis fungoides. There have been no adequate randomized controlled trials with sufficient power comparing this modality with other therapies. Objective To assess disease response and to compare the response rates of patients treated with PUVA alone or PUVA and bexarotene. Methods EORTC 21011 (NCT 00056056) was a randomized phase III study comparing combined bexarotene (Targretin®) and PUVA vs. PUVA alone in patients with stage IB and IIA mycosis fungoides (MF). The primary endpoint was the overall response rate [complete clinical response (CCR) plus partial response (PR)]. Results The study was prematurely closed due to low accrual after 93 of 145 required patients (65%) were randomized. Of the 93 randomized patients, 87 started treatment, 41 received PUVA and 46 received PUVA + bexarotene. Total UVA doses received were 107 J cm-2 (range 1·4-489·9) in the PUVA arm vs. 101·7 J cm-2 (0·2-529·9) in the combination arm. The safety profile was acceptable with few grade 3-4 toxicities observed in either arm. More drop-outs due to toxicity were observed in the combination arm compared with the PUVA-alone arm. The best overall response (CCR + PR) rate was 71% for PUVA alone and 77% for the combination arm (P = 0·57). The median duration of response was 9·7 months for PUVA vs. 5·8 months for the combination arm (P = 0·33). CCR was seen in 25 patients of whom 10 received PUVA alone (CCR 22%) and 15 received combination therapy (CCR 31%) (P = 0·45). CCR was sustained in 25% of patients regardless of therapy. There was a trend towards fewer PUVA sessions needed to achieve CCR in the combination arm (median 22) compared with the PUVA arm (median 27·5) (P = 0·11). Similarly, a trend towards lower UVA dose required to achieve CCR in the combination arm (median 55·8 J cm-2) compared with the PUVA arm alone (median 117·5 J cm -2) (P = 0·5) was observed. Conclusions No significant difference in response rate or response duration was observed in this study. However, there was a trend towards fewer PUVA sessions and lower UVA dose required to achieve CCR in the combination arm (PUVA + bexarotene) but this did not achieve statistical significance due to insufficient power. See also the Commentary by Ahmed and Piguet © 2012 British Association of Dermatologists. Source
Ignatiadis M.,Free University of Colombia |
Rack B.,Ludwig Maximilians University of Munich |
Rothe F.,Free University of Colombia |
Riethdorf S.,University of Hamburg |
And 16 more authors.
European Journal of Cancer | Year: 2016
There is increasing evidence that breast cancer evolves over time under the selection pressure of systemic treatment. Today, treatment decisions in early breast cancer are based on primary tumour characteristics without considering the disease evolution. Chemoresistant micrometastatic disease is poorly characterised and thus it is not used in current clinical practice as a tool to personalise treatment approaches. The detection of chemoresistant circulating tumour cells (CTCs) has been shown to be associated with worse prognosis in early breast cancer. The ongoing Treat CTC trial is the first international, liquid biopsy-based trial evaluating the concept of targeting chemoresistant minimal residual disease: detection of CTCs following adjuvant chemotherapy (adjuvant cohort) or neoadjuvant chemotherapy in patients who did not achieve pathological complete response (neoadjuvant cohort). This article presents the rational and design of this trial and the results of the pilot phase after 350 patients have been screened and provides insights that might provide information for future trials using the liquid biopsy approach as a tool towards precision medicine (NCT01548677). © 2016 Elsevier Ltd. All rights reserved. Source
Clinical and biological impact of TET2 mutations and expression in younger adult AML patients treated within the EORTC/GIMEMA AML-12 clinical trial
Aslanyan M.G.,Radboud University Nijmegen |
Kroeze L.I.,Radboud University Nijmegen |
Langemeijer S.M.C.,Radboud University Nijmegen |
Koorenhof-Scheele T.N.,Radboud University Nijmegen |
And 21 more authors.
Annals of Hematology | Year: 2014
We assessed the prognostic impact of TET2 mutations and mRNA expression in a prospective cohort of 357 adult AML patients<60 years of age enrolled in the European Organization For Research and Treatment of Cancer (EORTC)/Gruppo Italiano Malattie Ematologiche dell' Adulto (GIMEMA) AML-12 06991 clinical trial. In addition the co-occurrence with other genetic defects and the functional consequences of TET2 mutations were investigated. TET2 mutations occurred in 7.6 % of the patients and were an independent marker of poor prognosis (p=0.024). TET2 and IDH1/2 mutations strongly associated with aberrations in the DNA methyltransferase DNMT3A. Functional studies confirmed previous work that neither nonsense truncations, nor missense TET2 mutations, induced 5-hydroxymethylcytosine formation. In addition, we now show that mutant TET2 forms did not act in a dominant negative manner when co-expressed with the wild-type protein. Finally, as loss-of-function TET2 mutations predicted poor outcome, we questioned whether low TET2 mRNA expression in cases of AML without TET2 mutations would affect overall survival. Notably, also AML patients with low TET2 mRNA expression levels showed inferior overall survival. © 2014 Springer-Verlag. Source


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MABEN -- One of the most difficult plays to complete in basketball is a three-point play when a team makes a free throw, misses a free throw, secures the rebounds, and scores on the putback. When the Aberdeen High School boys basketball team completed the rare feat three times in the first half Saturday night, New Hope had to feel it may not be its night.
The Starkville High School girls basketball team didn't have an answer for one of the best front-court players in the state of Mississippi on Saturday afternoon. Junior Jazmine Spears scored 24 points to lead New Albany to a 45-42 victory against Starkville on the second day of the Joe Horne Christmas Classic at Columbus High.
Softball has been a part of Lauren Holifield's life ever since she can remember. She watched her mother, Connie Sharpe, coach at Caledonia High School and play the sport. She also grew up seeing her sister, Tori Harris, play for New Hope High and imagined the day she would wear the black and gold and help the program build on its tradition.
Tabitha Beard has found the perfect phrase: As long as you're doing something you love, it never will get old. She smiles when asked if being coach of the New Hope High School softball program gets old, especially on the heels of the slow-pitch team's fifth consecutive state title in October. The title pushed the program's number of championships to 14.
HATTIESBURG -- Southern Mississippi coach Larry Eustachy would have preferred that Neil Watson cut the fast break short, milking some clock in the final minute of a tight game against in-state rival Mississippi. The precocious point guard had other ideas. Watson flipped a pass to Torye Pelham, who was streaking down the lane and finished with a powerful, one-handed jam that sealed Southern Miss' 86-82 victory against the University of Mississippi on Saturday night.
DETROIT -- Arnett Moultrie and the No. 17 Mississippi State men' basketball team kept cool when it mattered most in their first true road game of the season. Hood made the go-ahead basket with 17.7 seconds to play and Moultrie added a big blocked shot seconds later to help MSU beat Detroit 80-75 for its 10th straight victory.
STARKVILLE -- Kendra Grant hopes she can keep the feeling. As shooters go, the Mississippi State freshman looks the part. She steps into her shots and has solid form and follow through to hit nothing but the bottom of the net from 3-point range.
Maggie Proffitt let the rock fly from beyond the 3-point line all night long. The junior Columbus High School girls basketball guard drained eight 3-pointers Friday night as the Lady Falcons routed Wingfield High School 63-26 in their first game of the 15th Annual Joe Horne Columbus Christmas Invitational.
The Columbus High School boys basketball team used a quick-paced offense and high-pressure defense to prevail Friday night. The Falcons held off Lawrence County High School 68-46 in the 15th Annual Joe Horne Columbus Christmas Invitational.
Starkville High girls basketball coach Kristie Williams continues to preach to her team the need to play for four quarters. When the Lady Jackets learn that lesson, the victories may indeed pile up.
STARKVILLE -- The Mississippi State women's basketball team had just about everything working for the first 5 minutes, 38 seconds Friday night against Southern Mississippi.
SCOOBA -- East Mississippi Community College sports fans will have an opportunity to relive some of the memories of the Lions' national championship football season this weekend with the airing of a half-hour special on WCBI-TV and My MS.
STARKVILLE --Mississippi State's football schedule for next season is now complete. After a six-month search to find a Football Bowl Subdivision opponent, MSU officials announced Friday afternoon a one-game contract to play Middle Tennessee State University at Davis Wade Stadium on Oct. 20.
STARKVILLE -- Dan Mullen knew his freshman quarterback would be exhausted, so after the open scrimmage at Davis Wade Stadium Friday night, he didn't need statistics to prove the important result. The Mississippi State football coach knew Dak Prescott had survived the most work behind center in his early college career.
STARKVILLE -- Mississippi State has already faced many unique challenges in the early part of the men's basketball season. The 17th-ranked Bulldogs (10-1) have already suffered a disappointing upset loss at home, won a tournament at Madison Square Garden, won games without key players and now have a new task. MSU must now figure out a way to win its first road game of the 2011-12 season.
The New Hope High School Trojans ousted the Caledonia High School Feds 76-64 Thursday night improving their record to 5-3. The Lady Trojans also came away with a victory beating the Lady Feds 49-29, improving their record to 10-0.
The "Twelve Days of Christmas" can mean several things to several people. However, for area high school basketball fans, it can mean plenty of quality matchups on the hardwood.
STARKVILLE -- Lack of depth is the result of trying to replace a pair of senior safeties that played nearly every defensive snap during a season. So it wasn't surprising to hear Mississippi State coach Dan Mullen admit that his team will be "a little young" in the back two members of the Bulldogs secondary.
For the first time ever, East Mississippi Community College can now boast a national Player of the Year in football. Less than a month after leading the Lions to a first-ever national championship, freshman quarterback Bo Wallace was named National Offensive Player of the Year by the NJCAA Thursday afternoon.
As the Christmas holidays begin on the Columbus High School campus, many students and teachers are looking forward to some time off and a chance to catch up with family and friends.
« previous Page 415 of 628 next »
1. Stovall, Self help MSU close weekend with two wins COLLEGE SPORTS
2. West Point girls beat New Hope for region title HIGH SCHOOL SPORTS
3. New Hope boys use defense to beat Oxford HIGH SCHOOL SPORTS
4. No. 3 MSU women survive in College Station, climb to top of SEC COLLEGE SPORTS
5. Ole Miss sweeps No. 6 East Carolina COLLEGE SPORTS


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August 14, 1995 Monday Night RAW results
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The August 14, 1995 Edition of Raw is War is a Professional wrestling television show of the WWF's RAW brand, took place on August 14, 1995 at the Worcester Centrum in Worcester, Massachusetts.
- Numbers in parentheses indicate the length of the match.
- (c) refers to the champion(s) heading into the match.
- Waylon Mercy defeated Doink the Clown
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- Henry Godwinn defeated Ross Greenberg
- Shawn Michaels defeated Jerry Lawler by DQ in WWF Intercontinental Championship match


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Westville is located in LaPorte county and has a population of 2,116.
English schools in Westville charged students an average tuition of $5,189.00 if they were Indiana residents, and $12,902.00 if they were non-residents, during the 2009 - 2010 school year.
The cost, on average, for books and supplies needed for english schools in Westville is $1,815.00. Students have a choice of one english college in Westville to attend. 10 students were reported to have graduated from english programs in Westville in the 2008 - 2009 school year.


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EA SPORTS UFC's Gameplay Series turns its focus to Bruce Lee and shares a look at some of his in-game skills.Fight as Bruce Lee: http://o.ea.com/21513 watch video >>
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In the third installment of EA SPORTS UFC's Gameplay Series, we turn up the volume for our Bruce Lee reveal. Music - Juelz Santana, The Second Coming.Pre-Order: http://o.ea.com/20199 watch video >>
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UFC's Dana White talks about why he almost got into a fight with Juanito Ybarra over Rampage Jackson watch video >>
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Dana White doing his first interview as an owner of the UFC. He look quite shy if you ask me :) watch video >>
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Description,,,Din Thomas form TNGF agrees that the Boxing box turns the heavy bag workout into a sparring session or a personal fight, instead of just hitting or doing drills on the heavy bag. Din Se watch video >>


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International Journal of Nursing Education Scholarship
Ed. by Andrusyszyn, Mary-Anne / Babenko-Mould, Yolanda / Goldenberg, Dolly / Cragg, Betty / Maltby, Hendrika J. / McWilliam, Carol
SCImago Journal Rank (SJR) 2015: 0.468
Source Normalized Impact per Paper (SNIP) 2015: 0.439
Impact per Publication (IPP) 2015: 0.667
- Online
Working with Standardized Patients: A Primer
1Fletcher Allen Health Care, (email)
2National Council of State Boards of Nursing, (email)
3Rush University College of Nursing, (email)
Citation Information: International Journal of Nursing Education Scholarship. Volume 4, Issue 1, ISSN (Online) 1548-923X, DOI: https://doi.org/10.2202/1548-923X.1437, August 2007
Publication History
- Published Online:
- 2007-08-22
This paper presents concepts and strategies for using standardized patients (SP) in teaching and evaluation of nursing students. SP encounters are an alternative to clinical experiences and a standardized criterion for student performance evaluation. Careful development of encounters, selection and training of SPs, support and debriefing of all participants are essential to a positive SP encounter. SP encounters should be developed based on objectives and competency criteria and relate to actual events. Encounter scripts incorporating any "traditional" language often associated with a specific medical condition are beneficial to standardizing the process. SP preparation involves providing background on medical conditions, feedback when practicing the role-play, and validation of performance consistency. Orientation of students and faculty to the SP experience ensures that participants stay in role. SPs can also be utilized to complete written evaluation tools and provide verbal feedback to students. All participants should evaluate the encounter process for future improvement.
Keywords: standardized patients; evaluation; student performance; simulation
Citing Articles
Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the "Citation Alert" on the top of this page.


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Check rates!
Free WiFi! WiFi is available in public areas and is free of charge.
Free parking! Free public parking is possible on site (reservation is not needed).
From 12:00 hours
Until 11:00 hours
All children are welcome.There is no capacity for extra beds in the room.
Pets are not allowed.
This property only accepts cash payments.
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Bike hire is available at the property and the area is popular for hiking. Holy Mother Virgins...
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Global Lean Manager
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Games & Hobbies, Video Games, TV & FilmFollow
Good Game is the show for gamers by gamers bringing you the latest news, reviews and interviews from the wonderful world of video gaming.
July 1st, 2013
Episode 107 of 262 episodes
On tonight's HEROIC episode of Good Game, Bajo and Hex are back in the chairs to look at Marvel Heroes, Company of Heroes 2, The Swapper and Deadpool...
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Welcome to the daily audio workout for your head.


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Delta Air Rallies on Plan to Curb 2016 Seat-Capacity Growthby
Lower fuel costs help overcome weakening airfares worldwide
A benchmark revenue gauge is still dropping, but more slowly
Delta Air Lines Inc. rose to its highest price in almost nine months after pledging to keep a lid on seating-capacity growth through next year to help regain pricing power against discount rivals.
The supply of available seats will increase by no more than 2 percent in 2016 after being unchanged this quarter, Delta said Wednesday. Matching the supply of tickets to travel demand is crucial, especially with some low-fare carriers taking advantage of falling fuel prices to pile on more flights.
"Investors were concerned about the industry's capacity growth as many believed the industry lost its discipline due to a lower fuel cost environment," Cowen & Co. analyst Helane Becker said in a note Wednesday. "Delta's 2016 capacity guidance will likely ease concerns of overcapacity."
Delta posted a third-quarter profit that beat analysts' estimates to open earnings reports for the U.S. industry in its busiest time of the year. With the rout in global crude markets cutting expenses for jet kerosene, analysts project that the six largest carriers will show a collective adjusted profit of $5.9 billion, a record for the period, according to estimates compiled by Bloomberg.
Good Season
"It's going to be a good season for the airlines," said George Ferguson, a Bloomberg Intelligence analyst. "They all have this gift of low fuel prices."
Delta gained 1.8 percent to $48.59 in New York, the highest closing price since Jan. 29. Delta was the best performer among the 11 stocks in the Bloomberg U.S. Airlines Index.
Delta's third-quarter earnings excluding some items were $1.74 a share, surpassing the $1.72 average of 15 estimates compiled by Bloomberg. Sales were $11.1 billion, Delta said Wednesday, matching the average estimate. Third-quarter net income more than tripled to $1.32 billion, or $1.65 a share, Delta said.
"Our revenue is basically flat year on year, and that's a result of the lower fuel, but we kept two-thirds of the lower fuel and put it to the bottom line," Chief Executive Officer Richard Anderson said in a Bloomberg interview.
Showing Improvement
Passenger revenue for each seat flown a mile will show improvement this quarter from previous periods, Delta said Wednesday. It's still dropping, though.
Carriers pay close attention to that benchmark gauge. When the seat supply expands -- especially with competition from low-fare operators -- carriers can struggle to raise prices.
For the fourth quarter, unit revenue will drop in a range of 2.5 percent to 4.5 percent, Delta said. That's better than the 4.9 percent decline for the third quarter, but still a sign of fare pressure for Delta and its peers at the top of the U.S. industry. For 2016, capacity will increase by no more than 2 percent, Delta said.
Weak Demand
Delta has moved to blunt the effects from weak demand overseas and the growth of U.S. discounters including Southwest Airlines Co. and Spirit Airlines Inc. In keeping seating capacity unchanged this quarter, it will offset U.S. expansion with an 11 percent reduction on Asia routes and a 1.5 percent cut to Europe.
The carrier also is trimming an unspecified number of office and management jobs worldwide. The cuts don't apply to pilots, flight attendants and other front-line employees.
Average U.S. coach fares fell to the lowest in almost five years in August, according to Airlines Reporting Corp. data compiled by Bloomberg, before rebounding in September.
At $1.80 a gallon, Delta's fuel cost was its lowest in years, compared with $2.90 a year earlier. The carrier also is moving past a series of wrong-way bets in recent quarters with hedging contracts.


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Maries Pizza Burleigh
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Maries Pizza is wholly Australian owned and operated. Our
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IMDB Rating: 8.3/10
Genre: Action / Adventure / Animation / Comedy / Crime / Family
Size: 800.1 MB
Runtime: 1hr 48 min
From the largest elephant to the smallest shrew, the city of Zootopia is a mammal metropolis where various animals live and thrive. When Judy Hopps becomes the first rabbit to join the police force, she quickly learns how tough it is to enforce the law. Determined to prove herself, Judy jumps at the opportunity to solve a mysterious case. Unfortunately, that means working with Nick Wilde, a wily fox who makes her job even harder.


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|Page (1) of 1 - 07/10/09
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Options Media Launches Proprietary Lead Generation PortalCompany Margins Expected to Improve (July 10, 2009)
BOCA RATON, FL -- (Marketwire) -- 07/10/09 -- Options Media Group Holdings, Inc. (OTCBB: OPMG), a leading Email Service Provider ("ESP") and Permission-based email, SMS/text messaging, and Lead Generation company, today announced it has launched its proprietary lead generation online advertising portal.
The Options Media Lead Generation (Lead Gen) Portal provides several ways for advertisers to put targeted offers in front of a buying consumer audience via a proprietary network of websites all specifically built by Options Media. Advertisers pay per generated lead on a cost per lead (the delivery of just the name and contact details) or cost per acquisition basis (an actual credit card paid transaction.)
The Options Media Lead Generation Portal is unique due to the daily site traffic that will be generated through email messaging from the Options Media Database as well as through the company's own ESP (Email Service Provider) clients.
An opted-in email user will receive an email with various incentives such as a chance to get a $50 Target gift Card, a Dell Laptop, an Apple iPod or a $50 Victoria's Secret gift certificate. Once the user clicks the link to get the incentive they are immediately sent into the Lead Generation Portal where they are presented with a variety of advertiser offers. Each time a user clicks a tab to receive further information, the advertiser is charged for a generated lead.
The network is managed within the Options Media Lead Generation division and utilizes advanced network technology and expertise. A dedicated account management and media team will work with advertisers from the education, health, financial and general consumer markets to place offers.
"The response to the Options Media Lead Gen Portal among advertisers and other Lead Gen companies during our initial launch period has been overwhelmingly positive," said Scott Frohman, CEO of Options Media. "They recognize the value of working with a Lead Gen partner that can guarantee ongoing traffic for increased exposure of their ads. The bottom line is that our portal will give advertisers greater return on their lead generation investment because their ads will be seen by fresh prospects each day."
"By utilizing our own portal network, internal email prospect database, as well as our email delivery platform, we will significantly increase our lead generation gross margins and our operating performance."
"The trend of increasing margins and closing in on positive cash flow is evident not just in our lead generation area, but in all areas of the company," explains Frohman. "As a matter of fact our permission-based email business is growing with campaigns being successfully implemented for companies such as Wynn Hotels, Palms Casino, Best Western Hotels, Jamba Juice, Army National Guard, Toyota Dealerships, Gerber, and other top brands just in the last few months. By adding this Lead Generation Platform to our existing email marketing capabilities, we are now able to generate a significant amount of leads with higher gross margins."
About Options Media Group
Options Media Group Holdings, Inc. is an email services provider for on-demand e-mail marketing to create, send, and track professional and permission-based e-mail marketing campaigns. Additionally, Options media provides precision direct marketing solutions including email marketing, SMS/mobile marketing, SMS/keyword marketing, custom lead generation and creative services. Options Media provides clients with access to software, hardware, bandwidth, and exclusive domains and IP addresses, as well as the ability to upload and manage subscribers, and review and upload campaigns and track results for a 360-degree full-service customer marketing solution.
Safe Harbor
This press release contains forward-looking statements within the meaning of the Private Securities Litigation Reform Act of 1995 (the "Act") including the prospects and potential for 2009 and anticipated 2009 cash flow. Additionally, words such as "seek," "intend," "believe," "plan," "estimate," "expect," "anticipate" and other similar expressions are forward-looking statements within the meaning of the Act. Some or all of the events or results anticipated by these forward-looking statements may not occur. Factors that could cause or contribute to such differences include economic issues relating to the current recession, potential advertising spending and potential consumer spending reductions, our ability to raise necessary working capital and the impact of competitive factors. Further information on Option Media Group's risk factors is contained in its filings with the Securities and Exchange Commission, including the Form 10-K filed on April 1, 2008. Options Media does not undertake any duty nor does it intend to update the results of these forward-looking statements.
Alliance Advisors, LLC
Bryan Kobel
Email Contact
Copyright @ Marketwire
Page: 1
Related Keywords: Options Media Group, Sales, Advertising, Marketing, Incentives, Sales & Marketing, Education, Database, Internet, Hardware, Other, Management, CEO/CFO, Prosumer/Consumer, Business, Marketing, Marketwire, Inc., Apple Computer, Financial, Business, Military, Email, Other,


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Harry Perrett (1897-1983)
218,607pages onAdd New Page
this wiki
this wiki
BiographyHarry Perrett was born 1897 in Raymond Terrace, New South Wales, Australia to James Ezra Perrett (1864-1950) and Mina Elizabeth Lancaster (1869-1936) and died 9 February 1983 in Inverell, New South Wales, Australia of unspecified causes. He married Elizabeth Hilda Avard (1893-1961) 1920 in Maitland, New South Wales, Australia. Ancestors are from Australia, the United Kingdom.
Sources and notes
|‡ General
|Ω Birth


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Crest Cadillac Collision Center
ReScore Reviews™ (1)
93% Would Recommend
Latest Review 10 months ago
Review for Crest Cadillac Collision Center
Devon H. on 05/22/2014
Devon said they did a pretty good job, but she was not satisfied with the preparation of the estimate. They had to change the estimate several times.
Category: Collision
Service Date: 05/08/2014
Review Created: 05/22/2014
This review was collected via phone interview by Customer Research Inc.


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I'm after some advice. I've been to see the "sleep doctor" Dr Brian Symons in Adelaide as my 3 mo baby does not settle well. He has suggested starting solids to assist with ensuring that bubs is gaining weight and therefore settling better.
Has anyone had any experience with starting solids earlier and have you found that it has helped with settling?


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Is this restaurant good for dining on a budget?
Does this restaurant offer highchairs for toddlers?
Does this restaurant offer validated parking?
Is this restaurant appropriate for Kids?
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Is this restaurant good for lunch?
Is this restaurant good for business meetings?
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Is this restaurant good for dinner?
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Thanks for helping!
It was a special family celebration for a dozen of us, the food was amazing, attentive staff, very reasonably priced in warm (log burners), exclusive, beautiful surroundings. Well done all!! Well worth a visit!!
Really bad customer service, a venue not to be trusted !
Booked a private room for a family meal, was told over the phone that this was fine and there was no charge to hire the room for 14 people. Called in to finalise details to be told by the very rude Owner' that they CAN NOT accommodate booking, unless...
We visited on valentines with a group of around 20 to celebrate my partners grandparents 60th wedding anniversary . We were in a private side room from the main dining room & when we arrived it was beautifully set with the fire lit - perfectly for a chilly day ! Vikki introduced herself and made us all feel at ease...
Thank you for your kind review, we are pleased you all had a lovely afternoon with us. We look forward to welcoming you all back soon.
Best wishes,
service and food was excellent, hot plates,plenty of choice. i ate in the resturant and also for the wedding we attended.
Thank you Penny! :)
We all hope to see you all again soon.
All the best.
Worst service have experienced for a long time and food not up to much. Soup was substituted for a different one without checking. Waitress kept disappearing for a smoke. No one around to check all okay
Firstly thank you for taking the time to leave a review.
We are so sorry to hear you did not enjoy your lunch and found the service to be poor. We will certainly be speaking to the members of staff who were on shift and not providing the service you should have received. We pride ourselves on our friendly...
totally agree with Richard B - went for sunday lunch kids supposed to be half price but got charged full price for them!!! also got charged astronomical amount for a bottled beer - when looked at the bar tariff - it wasn't listed at all so I couldn't question the price
when I queried what we'd been charged we were...
Please call us on 01789 731857 to help us resolve this for you. We were not aware of anyone who has left here after a Sunday lunch unhappy, or have been charged full price for children, or anyone who has queried any prices with us.
Kind regards,
Excellent quality. Food beautifully cooked - puddings are a bargain. Nice surroundings as well. Very close to Stratford upon Avon.
Hi, thank you so much for your kind review. We are pleased you had a lovely lunch. Hope to see you again very soon. All the best. Vikki
Top quality food in lovely surroundings, being part of the golf course and overlooking the green. A set Sunday lunch menu in operation of Carvery for £9.99 and 1p for a dessert with good choice of starters if required.
Roast beef, turkey and gammon today with little Yorkshire puddings, roast potatoes, roast parsnips, carrots, green beans, cauliflower cheese, stuffing balls...
Sorry for the delayed reply. Thank you for your kind review. We look forward to seeing you here again soon! Best Wishes Vikki
A group meal before Christmas - starter was poor, main course very poor. The waitresses were pleasant and helpful but management not prepared to discuss concerns. After trying to resolve matters with the supervisor eventually managed to speak with manager who was extremely rude and agressive. Some others in the group had better experience, but gather your strength before making...
Enjoyed our stay at ingon manor,we went as a group of 16',very nice golf course well maintained,we stayed 2 nights ,3 rounds of golf,single room £ 195.reasonable priced ,food very good,one problem only one staff doing the bar and serving meals first night,but 2 second night. Overall we enjoyed our visit,would go again.
Own or manage this property? Claim your listing for free to respond to reviews, update your profile and much more.


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Outlier presents a superior pair of chinos in with their new 'Autumnweight' version. The pants come well suited for the rain with its 325 gram twill fabric, offering four-way stretch for true freedom and movement. Each pair is built with added key loops, a stash pocket, and hidden front button. The pockets feature a linen-cotton blend for natural toughness in safari, navy and olive colors. Comfortable and in colors reminiscent of this season, Outlier has the item available now for $225 USD.


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An Iranian scientist has invented a time machine that goes backward and forward in time!
The World's Only Reliable News
An Iranian scientist has invented a time machine that goes backward and forward in time!
Iran is planning to bomb Hollywood over the Oscar-winning movie Argo because of its 'unrealistic portrayal' of the country.
An Iranian spokeswoman confirmed last night Mahmoud Ahmadinejad is an imposter.
BREAKING NEWS – Osama bin Laden was spotted in Tehran yesterday!
TEHRAN – Iran announced that it has built the world's first flying saucer.
JORDAN – Mideast leaders agree – Satan has taken control of Hillary Clinton's body.
HAVANA – Fidel Castro admitted that the Cuban system doesn't work and hopped a raft for America.
FRANCE – Carla Bruni is back in the news. WWN first revealed that Bruni was immortal and was far beyond your mere first lady. Stay-tuned as more will follow tomorrow!
TEHRAN – Despite repeated crackdowns, protests in Iran continue. To calm and distract the populace, Ahmedinejad staged a tribute to Michael Jackson.
TEHRAN – While violence continues to rock Iran over election results, Mahmoud Ahmadinejad went into a funk after President Obama never returns his calls.


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John Caldigate
John Caldigateby Anthony TrollopeAbout Trollope:Anthony Trollope's father, Thomas Anthony Trollope, worked as abarrister. Thomas Trollope, though a clever and well-educated manand a Fellow of New College, Oxford, failed at the bar due to hisbad….. Read more
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Home | Shop | Personal Care | Oral Care | Dental Accessories | DenTek Easy Brush Standard, 16CT
DenTek Easy Brush Standard, 16CT
|Special Info for This Item
|FREE Shipping Eligible
|Check Store Availability
The DenTek Easy Brush interdental brush makes it easy to brush the hardest-to-reach spaces of your mouth.
- Only interdental brushes on the market with an advanced fluoride coating for a deep clean between teeth
- Durable tapered brush cleans tight spots and is great for braces
- Flexible wire allows bristles to bend for better access
- Flexible handle holds position when bent
- Minty flavor leaves your mouth feeling fresh
- Anti-microbial cap keeps brush clean between uses and fits on grip to extend handles reach
- Quantity:
- Primary Flavor:
- Mint
- Insert between teeth, interdental spaces, or dental appliances.
- Use a soft back-and-forth motion to remove plaque and food particles.
Caution: Do not force. Under the age of 10 requires adult supervision.


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The countdown is on. With just over one week remaining until our third annual #RFGoNaked Day, Dr. Katie Rodan and Dr. Kathy Fields are calling on you to go naked (from the neck up).
Flaunt your au naturel complexion by posting a makeup-free selfie on Friday, July 8. For every selfie, Rodan + Fields® will donate $5 through the Prescription for Change® (PFC) Foundation to our nonprofit partner, buildOn, to help support students in need. The goal is to Engage kids in school and Empower their future!
Click on the video link to hear from Drs. Rodan and Fields about this great event!
Do it for a great cause!
Happy Selfie's!


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Home » Accessories » Skins » FPCKS15
Fujitsu America, Inc. Fpcks15 Kybd Skin T4410 T4310
Oops! It appears we are out of stock or no longer selling this product.
Model: FPCKS15
Search Beach Audio for similar products
Warranty Information
|Beach Audio Warranty
This product was added to our catalog on Thursday, August 11, 2016.


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[Selling] Honor Above All: A Tapestry Of Trinsic
Discussion in 'UO Rares Collector' started by Dregg, Dec 18, 2014.


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Forum indexCanon Rebel (EOS 300D-800D) TalkChange forum
Why corrupt .CR2 file from 350D (NOT Lightroom caused).
Started Jan 5, 2013 | Discussions thread
Shop cameras & lenses ▾
stuartp56 • Regular Member • Posts: 174
Why corrupt .CR2 file from 350D (NOT Lightroom caused).
Jan 5, 2013
I can see thumbnail when Adobe Bridge begins to recognize the image, then it goes black. File is within the normal size range. Normal download with cable. I do NOT use Lightroom. I've taken over 59,000 photos with this Rebel XT/350D and never seen anything like this.
I downloaded the file again with a card reader using a recovery software because I'd deleted the files from the card and got the same result so it's the image not the download method.
Can I fix the image? But more critical: what caused the problem, so I can avoid it in the future.
stuartp56's gear list:stuartp56's gear list
Canon EOS 350D Canon EOS Rebel T4i Canon EF-S 18-135mm f/3.5-5.6 IS Canon EF 75-300mm f/4.0-5.6 III USM Canon EF-S 18-55mm f/3.5-5.6 II +1 more
Canon EOS 350D (EOS Digital Rebel XT / EOS Kiss Digital N)
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Sold out
Boys Yellow Cotton Jersey 'Monster' T-Shirt
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Boys, short-sleeved, bright yellow t-shirt by Fendi. Made in soft cotton jersey, it has the designer's signature monster 'Bag Bugs' eyes and logo printed on the front.
- Product number 127320
- 100% cotton (soft jersey feel)
- Machine wash (30*C)
- True to size fitting
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These Product FAQs pertain to RUO products. These products are for Research Use Only. Not for use in diagnostic procedures. If using an IVD product, please refer to your specific product information.
What points should I consider to achieve optimal transfection with a lipid-based transfection reagent?
Product FAQ
Here are some points to consider:
1. Select the cationic lipid reagent that is likely to result in highest transfection efficiency for your cell type. Please refer to the Transfection Reagent Selection Guide (https://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/transfection-reagent-application-table.html) to make the right choice.
2. Optimize the cationic lipid reagent and DNA amounts. The most important parameter after the condition of the cells is the ratio of lipid to DNA.
3. Do not use serum during complex formation. Serum may contain components that could interfere with complex formation. We recommend using Opti-MEM™ I Reduced-Serum Medium for optimal complex formation. However, serum-free DMEM or serum-free RPMI 1640 Medium can be used, keeping in mind that the efficiency of complex formation may not be as high as with Opti-MEM™ I Reduced-Serum Medium.
4. Do not use antibiotics, EDTA, citrate, phosphate, chondroitin sulfate, hyaluronic acid, dextran sulfate, or other sulfated proteoglycans in the medium used to prepare the DNA-cationic lipid reagent complexes.
5. Cell density should be from 50% to 80% confluency at the time of transfection (for Lipofectamine™ 2000, we recommend >90% confluency). Cells should be in the mid-log growth phase. For better consistency of results between transfection experiments, it would be best to accurately count your cells with a hemocytometer or with the Countess™ II FL Automated Cell Counter (Cat. No. AMQAF1000).
6. Make sure that the promoter-enhancer of the transfected DNA is compatible with the target cell type.
7. Do not use a cationic lipid reagents that has been frozen or stored in a section of the refrigerator where the temperature is below 4 degrees C.
8. Include a positive control for the transfection assay (for example, Cat. No. A14146 for plasmid DNA transfection and Cat. No. 14750100 for siRNA transfection).
Also, please take a look at the tips outlined here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/transfection-support/troubleshooting-transfection-experiments.html).
Answer Id: E8989
Was this answer helpful?
What controls do you recommend that I use when performing a transfection?
Product FAQ
In general, our recommended controls include:
- Cells only
- Cells + DNA or RNAi only
- Cells + lipid reagent only
- Cells + GFP plasmid positive control
These controls are important as they check cell health, reagent toxicity, and reagent function simultaneously.
Answer Id: E8990
Was this answer helpful?
Are cell density (% confluency) and passage number important considerations for transfection?
Product FAQ
Yes. Cell density will affect transfection performance. Lipofectamine™ 3000, Lipofectamine™ 2000, and Lipofectamine™ LTX/PLUS provide excellent transfection performance at confluencies between 70 and 90%, while some toxicity may be observed at confluencies lower than this. Lipofectamine™ RNAiMAX works best at confluencies between 60 and 80%.
Passage number may affect transfection experiments. We recommend consistent splitting and plating of cells. Excessive numbers of passages may decrease transfection performance. We do not recommend splitting cells for more than 20-30 passages. If transfection performance declines and cells have been in culture for a long time or excessively/improperly passaged, we recommend that you restart your cultures with a new vial of cells from liquid nitrogen. Please refer to the Gibco™ Cell Culture Basics handbook (https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics.html) for proper guidelines for culturing and passaging cells.
Answer Id: E8962
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I don't see a cell line-specific transfection protocol. What conditions should I try?
Product FAQ
If you do not find a cell line-specific protocol or if the protocol does not perform as expected, we recommend testing the conditions described in the protocol supplied with the product to determine the optimal protocol. Successful transfection depends on the cell type, amount of lipid, cell health, passage number, and cell density at the time of transfection. Each of these factors may differ slightly from lab to lab, and require additional optimization of the protocol to achieve the same result.
Answer Id: E8992
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Can I use the same amount of any transfection reagent for different cell lines?
Product FAQ
No. The transfection efficiency is highly dependent on the amount of reagent used per well and may be different between reagents. Please consult the product information that is provided with the transfection reagent for optimal use. The protocol that is supplied with the product will provide you with an optimal range of transfection reagent to use per well. During product development, this range was determined to work well across a variety of cell lines. If you are still not achieving the performance you desire in your particular cell line, further optimization may be necessary.
Answer Id: E8963
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How do I choose between Lipofectamine™ 2000 and Lipofectamine™ LTX?
Product FAQ
Lipofectamine™ LTX and Lipofectamine™ 2000 are both recommended for plasmid DNA transfections. However, if you notice increased cytotoxicity with Lipofectamine™ 2000, Lipofectamine™ LTX is the preferred reagent due to significantly reduced cytotoxicity levels and improved transfection performance in a number of hard-to-transfect cell lines.
Answer Id: E4997
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Which lipid transfection reagent would you recommend trying for my cell line?
Product FAQ
We recommend using Lipofectamine™ 3000 Reagent or delivery of plasmid DNA, Lipofectamine™ MessengerMAX™ Reagent for delivery of mRNA or short oligos, and Lipofectamine™ RNAiMAX Reagent for delivery of siRNA/miRNA.
Answer Id: E8993
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I am working with well sizes different from those specified in your protocol. How do I scale up or scale down my transfection reaction?
Product FAQ
Each of our transfection reagent protocols provides a table for scaling up or down transfections. Please consult the specific manual for details.
Answer Id: E8964
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In the case of the 96-well protocol, Lipofectamine™ 2000 requires 320 ng DNA, whereas Lipofectamine™ PLUS™, or Lipofectamine™ LTX requires up to 200 ng DNA. Does this mean that more DNA is needed to transfect efficiently when using Lipofectamine™ 2000?
Product FAQ
Yes, you do need more DNA per well when using Lipofectamine™ 2000, because the optimal confluency of the cells is 90% (as compared to 50-80% confluent with Lipofectamine™, or Lipofectamine™ LTX. Therefore you need more DNA in order to ensure that the maximum number of cells are transfected. With Lipofectamine™ 2000, we have found empirically that the higher confluence (~90%) of cells at the start of transfection improves transfection efficiency.
Answer Id: E3062
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Is it necessary to use serum-free media during lipid transfection?
Product FAQ
Not in all cases. What is essential is to form the lipid:nucleic acid complex in the absence of serum, because proteins can interfere with complex formation. Once the complexes are formed, they can be added to cells in serum containing medium. For optimal results, with Lipofectin™ perform transfection in medium without serum.
Answer Id: E3131
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Why are my transfections not reproducible?
Product FAQ
Please review these possible causes for non-reproducible transfections, along with suggested solutions (http://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/transfection-support/troubleshooting-transfection-experiments.html#3). Also, we recommend setting up one master mix of the DNA/lipid complex for the number of transfections planned to reduce multiple pipetting errors. When adding your complexes, we recommend changing tips between wells since re-used tips could bring carryover, especially for the 96- or 384-well format with small-volume formats.
Answer Id: E8983
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Why is my transfection not reproducible?
Product FAQ
In general, transfection efficiency will show some degree of variability from one transfection to another, no matter how hard one tries to control the parameters of transfection. Keep all transfection parameters, such as cell confluency, passage number, and phase of growth, consistent between transfections. If possible, thaw fresh cells. To minimize the effect of transfection variability, one can use an internal reference control such as beta-galactosidase or luciferase. Co-transfect the expression plasmid with the reference plasmid and assay for the activity of beta-gal or luciferase.
Answer Id: E8965
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Can antibiotics be used in the medium during transfection?
Product FAQ
Yes, antibiotics (penicillin-streptomycin) can be used in the medium during transfection. We have compared transfecting cells in medium with and without antibiotics in multiple cell lines, assessed both the transfection efficiency and toxicity, and found no difference. For certain cell types that are sensitive to transfection or have toxicity issues, omitting antibiotics may improve results, however. For stable transfections, wait at least 72 hours after transfection before adding selective antibiotics.
Answer Id: E8998
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Are lipid transfection reagents fluorescent?
Product FAQ
The intrinsic fluorescence of Lipofectamine™ 2000 and Lipofectamine™ under FITC conditions was examined and the results were as follows:
- Lipofectamine™ 2000 in serum-free conditions (with or without DNA) - weak whitish fluorescence.
- Lipofectamine™ 2000 in serum-containing conditions (with or without DNA) - medium white and red fluorescence.
- Lipofectamine™ & Lipofectamine™ PLUS (with or without DNA) - orange fluorescence.
- We haven't seen any significant green fluorescence attributable to the Lipofectamine™.
These observations were made after transfection of CHO-K1 cells and by examining the live cells in PBS. Other lipid transfection reagents were not studied extensively on this issue, but it is very likely they will produce fluorescence in transfected cells.
Fluorescence from various media components may be where the problem lies. For example, riboflavin apparently fluoresces in the green wavelength and thus DMEM, which has a high riboflavin content, can be problematic in fluorescence experiments. Ham's F12 medium is 10x lower in riboflavin and is more suitable for fluorescence work.
One possible solution is to perform fluorescence imaging in PBS, rather than in culture medium. Also, check to make sure the cells are healthy and intact. Lysed cells or cells under stress may generate autofluorescent products.
Answer Id: E4451
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Is my lipid (Lipofectin™, Lipofectamine™, DMRIE-C, Lipofectamine™ 2000, Oligofectamine™, Cellfectin™ II) supposed to be cloudy?
Product FAQ
It is normal to see some turbidity and cloudiness in DMRIE-C, Cellfectin™ II and Lipofectamine™ 2000, and the others should be clear. Lipid transfection reagents are sensitive to low temperature; if you put them at temperature below 4 °C they will precipitate or even freeze and lower the efficiency. Most lipids will go cloudy and precipitate upon freezing, and may not be active anymore.
Answer Id: E4000
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text:
In the last 90 days, 13 listings have been listed, which means new listings most likley are leasing quickly. You'll want to be prepared to make an offer immediately. There aren't many listings below 2,000 square feet posted, so finding a space for a small business might be tough.
The standard measurement for office space in this area is around 410 sqft. Most properties in this market are approximately 1 stories. Most buildings in this region were built in 1975 and are Class B.
Not much of the real estate is used for commercial space, so it might be hard to find office space. Utilities is the dominant industry in this area. Most of the residents of Delano work from home. Commuting close to 30 minutes a day is the norm for those who opt to reside in this part of town.
Trends and status of the commercial real estate market in Delano.
Market Report
Real time and historical data on commercial real estate listings in Delano.


text:
Hi Lynn!
I agree with Dorothy's advice on the soft rot. I too have had a bit of trouble with that due to the wet spring, and also some trouble with fungal leaf spot. This was my first time battling leaf spot -- guess I have been very lucky til now. I sprayed with Daconil last week for that since I had trouble on some lilies with another fungus that is supposed to respond to Daconil anyhow. Hope you avoid the leaf spot! ( I see by your signature that you are a D.V.M. -- me too, although that was a long time ago and I never practiced much due to a child with some disabilities to take care of. Do you do small animals?)
"Lynn Stucker, DVM" <firstname.lastname@example.com> wrote:
Hi everyone,
I have just made it through my first season of my TB's blooming. I have kept
up with removing spent blooms and stalks, but am wondering what else I need to
do to keep the garden healthy. Here in S. WI we have had an extremely wet
spring and I have lost some fans due to it being too wet and the soil washing
up around and ontop of the rhizome. Could I get some advice from all of you
experts on what I should do. Trim dead fans back? Time to divide? Fertilize?
I would appreciate any suggestions and knowledge anyone cares to pass along.
Thank you.
Greendale, WI
zone 5
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Carole Taber
Beaver Dam, Wisconsin
Zone 4/5
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Can a vegan person get a good meal at this restaurant?
Can a vegetarian person get a good meal at this restaurant?
Does this restaurant offer takeout or food to go?
Can a gluten free person get a good meal at this restaurant?
Is this restaurant good for dinner?
Is this restaurant good for breakfast?
Is this restaurant good for lunch?
Does this restaurant specialize in Halal food?
Is this a Kosher restaurant?
Does this restaurant offer validated parking?
Thanks for helping!
3 Days in Gran Canaria
Guide to Gran Canaria for Families
One Day in Gran Canaria
I have been twice there only for drinks so I can't say what the food is like. But the service and the drinks was really nice.
If you like a hectic staff, shouting to each other and if you like cordial behaviour in a limited version of your mother tongue, this is the place for you.
We had king prawns with nice vegetables and really tasty sauces. Accompanied by a dry Spanish white wine. The meal itself was delicious, but the feeling of being part of...
Mogan Mar on beachfront and it's sister Mogan Mar II right across the road from Cordial Valle - very friendly welcoming staff and a great menu choice. Food lovely and very reasonable priaced.
We have been visiting Gran Canaria since 2010 and during our first visit we discovered Puerto de Mogan and this has become one of our favourite places in the world. A visit to Mogs has also been a favourite since then. Great spot to sit and people watch / watch the world go by. Great coffees / wines / beers...
We went back 3 times to this restaurant as we enjoyed it so much, the meat kebab was lovely, and staff were very friendly, they also serve really nice coffee which is difficult to find in any of the others.
Friendly service, you have to wait but it is a good thing as the food is cooked fresh. Plenty of good quality food, only downside is people smoke whilst you are eating, but if you are used to that or don't mind people smoking than a good place to eat
We are visiting Gran Canaria for the first time and staying in Puerto Mogan. A friend who visits regularly booked an Anniversary meal for us here. We arrived to a table festooned with hearts and ribbons. Our meal was lovely. I had the mixed kebab which was huge! Lovely and tender. With desert they had sparklers and wished us Happy...
Love Mogan, and love Mogan Mar . What a fabulous restaurant. Ok the tour for business as you pass by, they all do on that strip.
Food and entertainment from the waiting staff first class.
I had the barbecue spare ribs, my wife had a steak in mushroom Sauce. Cooked to perfection. Presentation perfect.
Drinks are cheaper in Mogan, and...
Having been to Mogan Mar three times last year I regret having to post a negative review, however the whole experience was well below par. Firstly, our order arrived wrong. The restaurant dealt with this ok but when the correct food arrived it was, well, disappointing. Mainly because both our dishes were rather tasteless (rather than full of flavour which...
We ate here one night, son and I and the mixed kebab and husband mixed grill. All the food was very nice, nice polite staff
Own or manage this property? Claim your listing for free to respond to reviews, update your profile and much more.


text:
I've just downloaded Bootstrap 3 from https://github.com/twbs/bootstrap. I'm watching this tutorial: http://www.youtube.com/watch?v=I6EPArp4csA. How exactly do I get started with modifying the LESS files?
What I did was to copy these directories: LESS, dist/css dist/fonts dist/js and create my new project. The current project folder looks like this:
with the HTML file having this at the top:
<link rel="stylesheet/less" type="text/css" href="less/my_site.less"/> //only contains @import "bootstrap.less";
<script src="js/less.js" type="text/javascript"></script>
<script src="js/jquery.js" type="text/javascript"></script>
<script src="js/bootstrap.min.js" type="text/javascript"></script>
Now when I try to modify one of the variables in variables.less and compile it with SimpLESS there's a Syntax Error: carousel.less. What gives? When I comment out "carousel.less", another problem pops up with dropdown.less. So maybe it has something to do with the mixins? Anybody have an idea on how I get started on moving on with this?
Try below links which will get you through the basics for customizing the twitter bootstrap.I also had problem while learning the twitter bootstrap in the beginning.But once learned you will love it for sure :)
EDIT: Jan 1, 2014
Give Koala a try. It compiles your LESS files real-time. It is free and cross-platform. You might also want to take a look at this FAQ about compiling Bootstrap with Koala.
Original Answer
I reproduced your problem with the app you mentioned. I tried another app such as crunch app and it worked fine. So with crunch, just drag and drop the
bootstrap.less file into that app and click on
crunch file button on the top right corner of the app. Then it will ask you where to save it. I personally use the command line tool assets-packager (
npm install -g assets-packager). I hope that helped.
if you have a php server, just install lessphp:
and then add this to your first line:
require "lessc.inc.php";
$less = new lessc;
$less->checkedCompile("stylesheet.less", "stylesheet.css");
and all you have to do is work with your less file and php will do the rest =')
These are some of the better tutorial I could find about Bootstrap 3 Less Workflow.
And if you want to learn Less, you can refer these links
Hope this helps.
Similar Questions


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Carry-out fried fish and chips.
1605 Race St.
Mon-Sat, 11am-9pm
Easy, casual sushi.
600 Vine Street
Splitsville offers an upscale bowling experience with a nostalgic feel, with eight luxury lanes and indoor and outdoor seating. Howl at the Moon provides a high energy live entertainment experience Wednesday through Saturday, with a unique show centered dance hits performed on pianos, guitars, drums and more.
145 Second St.
Mon–Fri, 4pm-2am; Sat–Sun, 11:30am-2am
Japanese Gastropub and sushi bar.
1400 Vine
Tues-Fri, 4pm- 10 pm; Fri, 4pm -11 pm; Sat 12pm-11 pm; Sun 12pm - 9pm
Offering a menu that changes daily with more than 30 seafood options, hand-crafted cocktails, and a focus on customer service. Ask about the $1.95 happy hour menu. Outdoor dining. Valet parking available. A 160-seat banquet facility is located in the Westin Hotel.
21 E. Fifth St.
Mon-Fri, 11am-11pm; Sat, 12pm-11pm; Sun, 4-10pm
M-F 4-6:30pm, M-Th 9:30pm-11pm. $1.95-$4.95 appetizer menu.
F, Sat kitchen until 11pm
Eclectic, farm-inspired menu focuses on foods and beverages of Spain and Latin America, with tapas, ceviches, cured hams, cheeses and large plates.
100 W. Fifth St.
Mon-Thu, 5-10 pm; Fri -Sat, 5-11 pm
Classic sushi rolls, hand rolls and special rolls. Located on Sixth Street along Restaurant Row.
580 Walnut St.
Mon-Thu, 11am-10pm; Fri-Sat, 12pm-12am; Sun, 4-10pm
Known for its USDA Prime Steaks, exquisite cuisine, extensive wine selection and outstanding service, diners can relax and enjoy the ambiance, while the staff offers a truly personalized dining experience.
Mon-Fri, 11am-10pm; Sat, 4-10pm
Mon-Fri, 5pm-11pm; Bar opens daily at 4pm
Full menu of healthy vegetarian and vegan dishes that include sushi and stir fry.
124 W. Elder St.
Tue-Sat, 9am-6pm; Sun, 10am-4pm
Seafood and oyster bar. Outdoor dining.
1401 Race St.
Tue-Thu,11:30-2, 5-10pm; Fri-Sat,11:30-2, 5-11pm
Dining and a bar in a restored turn-of-the-century saloon. Specializes in oysters.
1000 Elm St.
Sun-Thu, 11am-9pm; Fri-Sat, 11am-11pm
find the hottest spots to eat in downtown cincinnati
tons of variety for the savy shopper
from charming to modern, downtown has it all
take in a ballgame, see art or enjoy some family time
move downtown and experience urban living
find the best places to park in downtown cincinnati
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text: Busty MouseJason Chen8/21/06 6:00pmFiled to: GadgetsMouseBoobs3EditPromoteShare to KinjaToggle Conversation toolsGo to permalink Now not only can you have a Busty Mousepad, you can have a busty mouse to go along with it. The mouse has two buttons and a scrollwheel—which part of a woman's anatomy is that—and costs only $4.99 (1000 Yen). Though, the breasts look more like eyeballs than breasts, so it's not that erotic. We found this on slashgear's new Japan site, catering towards Japanese-reading gadget fans. Go have a look. Advertisement Advertisement Amorousness mouse [jp.slashgear]Reply3 repliesLeave a reply