text:
issue contents
September 2004 issue
Cover illustration: YscM2 (green ribbon), a negative regulator of type III secretion in Yersinia enterocolitica, binds to the homodimeric secretion chaperone SycH (molecular surface rendering showing the distribution of electrostatic potential) in a manner that is very similar to the way in which the bacterial cytotoxin YopE (yellow ribbon) interacts with its cognate secretion chaperone SycE (not shown). SycH evidently unfolds YscM2 and presents it to the type III secretion apparatus in an extended conformation (p. 1591).
Acta Cryst. (2004). D60, 1509-1511
doi: 10.1107/S0907444904018189
doi: 10.1107/S0907444904018189
Obituary for Carl-Ivar Brändén.
research papers
Acta Cryst. (2004). D60, 1512-1518
doi: 10.1107/S0907444904014593
doi: 10.1107/S0907444904014593
DsbC forms a flexible dimer that can accommodate a wide variety of protein substrates for disulfide-bond isomerization.
PDB reference: DsbC, 1t3b, r1t3bsf
Acta Cryst. (2004). D60, 1519-1526
doi: 10.1107/S0907444904014969
doi: 10.1107/S0907444904014969
Ab initio phasing based on topological restraints: automated determination of the space group and the number of molecules in the unit cell
Dependence of ab initio connectivity-based phasing on its principal parameters allows an automated indication of the space group and of the number of molecules in the unit cell.
Acta Cryst. (2004). D60, 1527-1534
doi: 10.1107/S0907444904015094
doi: 10.1107/S0907444904015094
The crystal structure of apo protein tyrosine phosphatase 1B has been determined to 1.95 Å resolution. The structure shows four highly coordinated water molecules in the active site of the enzyme and the WPD-loop in a closed conformation.
PDB reference: apo protein tyrosine phosphatase 1B, 1sug, r1sugsf
Acta Cryst. (2004). D60, 1535-1544
doi: 10.1107/S0907444904015252
doi: 10.1107/S0907444904015252
Main-chain molecular fragments are positioned in electron density by a phased rotation and translation function. The fragments are refined as flexible generalized atoms and connected into chains. The sequence is aligned and side chains are built. The overall accuracy of the protein model is about 0.2 Å at a resolution of 1.2–1.9 Å.
Acta Cryst. (2004). D60, 1545-1554
doi: 10.1107/S0907444904015422
doi: 10.1107/S0907444904015422
Structure of apo acyl carrier protein and a proposal to engineer protein crystallization through metal ions
To demonstrate a new strategy for engineering crystal lattices through metal-mediated interactions, apo acyl carrier protein has been crystallized in the presence of zinc ions. The structure was solved with MAD phasing using zinc anomalous signals.
PDB reference: apo acyl carrier protein, 1t8k, r1t8ksf
Acta Cryst. (2004). D60, 1555-1561
doi: 10.1107/S0907444904015628
doi: 10.1107/S0907444904015628
Shrimp alkaline phosphatase has been subjected to inhibitor and metal-exchange studies, which induced conformational changes in the active site.
PDB references: SAP–PNPP complex, 1shn, r1shnsf; SAP–Mg complex, 1shq, r1shqsf
Acta Cryst. (2004). D60, 1562-1568
doi: 10.1107/S0907444904015732
doi: 10.1107/S0907444904015732
A statistical design approach is proposed to screen for and optimize protein crystallization conditions.
Acta Cryst. (2004). D60, 1569-1578
doi: 10.1107/S0907444904016798
doi: 10.1107/S0907444904016798
The crystal structure of the first human parasite superoxide dismutase is described. Alternate positions for the catalytic copper are described along with differences within the electrostatic loops used for substrate guidance.
PDB references: Cu,Zn superoxide dismutase, 1to4, r1to4sf; 1to5, r1to5sf
Acta Cryst. (2004). D60, 1579-1585
doi: 10.1107/S0907444904016786
doi: 10.1107/S0907444904016786
Observation of time-resolved structural changes by linear interpolation of highly redundant X-ray diffraction data
Linear interpolation of X-ray intensities from redundant data sets was used to determine the structures of insulin at different time points throughout the data collection. The structures reveal time-dependent structural changes that result from radiation exposure.
Acta Cryst. (2004). D60, 1586-1590
doi: 10.1107/S090744490401697X
doi: 10.1107/S090744490401697X
A redetermination of the structure of the triple mutant (K53,56,120M) of phospholipase A2 at 1.6 Å resolution using sulfur-SAS at 1.54 Å wavelength
The crystal structure of the triple mutant (K53,56,120M) of a small protein (14 kDa), bovine pancreatic phospholipase A2, has been redetermined using sulfur single-wavelength anomalous scattering (S-SAS) at 1.6 Å resolution using a wavelength of 1.54 Å.
PDB reference: K53,56,120M PLA2, 1vkq, r1vkqsf
Acta Cryst. (2004). D60, 1591-1599
doi: 10.1107/S0907444904017597
doi: 10.1107/S0907444904017597
Structure of the Yersinia pestis type III secretion chaperone SycH in complex with a stable fragment of YscM2
The structure of the type III secretion chaperone SycH from Y. pestis has been solved in complex with a stable fragment of YscM2, a negative regulator of type III secretion.
structural genomics papers
Acta Cryst. (2004). D60, 1600-1605
doi: 10.1107/S0907444904015240
doi: 10.1107/S0907444904015240
The structure of serine acetyltransferase from H. influenzae Rd determined at 2.7 Å resolution is presented. The structure is a hexamer consisting of a dimer of trimers, each of which contains subunits characterized by a conserved N-terminal α-helical domain and a C-terminal left-handed parallel β-helix.
PDB reference: serine acetyltransferase, 1s80, r1s80sf
Acta Cryst. (2004). D60, 1606-1610
doi: 10.1107/S0907444904017603
doi: 10.1107/S0907444904017603
The crystal structure of BAG-1 protein from C. elegans reveals a new BAG-domain fold.
PDB reference: BAG-1, 1t7s, r1t7ssf
crystallization papers
Acta Cryst. (2004). D60, 1611-1613
doi: 10.1107/S0907444904015033
doi: 10.1107/S0907444904015033
Cloning, expression, purification, crystallization and initial crystallographic analysis of transcription factor DksA from Escherichia coli
Transcription factor DksA from E. coli, which contains a canonical Zn-finger motif, was cloned expressed, purified and crystallized. The crystals diffract beyond 2.0 Å resolution at a synchrotron and three complete multiple anomalous dispersion data sets were collected at the absorption edge of the Zn atom.
Acta Cryst. (2004). D60, 1614-1617
doi: 10.1107/S0907444904015343
doi: 10.1107/S0907444904015343
Cloning, expression, crystallization and initial crystallographic analysis of the C-terminal domain of the amyloid precursor protein APP
The C-terminal domain CAPPD of the β-amyloid precursor protein, a type I transmembrane protein involved in Alzheimer's disease, was cloned, expressed in E. coli and, after purification, also crystallized. The initial electron-density map was of good quality and showed mostly helical regions.
Acta Cryst. (2004). D60, 1618-1621
doi: 10.1107/S090744490401666X
doi: 10.1107/S090744490401666X
Expression, purification, crystallization and preliminary X-ray diffraction studies of the cmcI component of Streptomyces clavuligerus 7α-cephem-methoxylase
The expression, purification, crystallization and preliminary X-ray diffraction studies of the gene product of cmcI from S. clavuligerus is reported. CmcI is believed to control the conversion of cephalosporins to cephamycins via 7α-methoxylation.
Acta Cryst. (2004). D60, 1622-1624
doi: 10.1107/S090744490401515X
doi: 10.1107/S090744490401515X
Crystallization and preliminary X-ray analysis of carboxypeptidase Y inhibitor IC complexed with the cognate proteinase
Carboxypeptidase Y inhibitor IC, complexed with the cognate proteinase, was crystallized and X-ray diffraction data were collected to 2.7 Å resolution.
Acta Cryst. (2004). D60, 1625-1627
doi: 10.1107/S0907444904015276
doi: 10.1107/S0907444904015276
Two new HIV proteases isolated from Brazilian patients failing intensive anti-AIDS therapy, namely subtype B mutant and subtype F wild type protein, were crystallized. The crystallization procedure and the molecular replacement solution for both proteins are presented in this paper.
Acta Cryst. (2004). D60, 1628-1631
doi: 10.1107/S0907444904015380
doi: 10.1107/S0907444904015380
Purification, crystallization and preliminary crystallographic data of the m3G cap-binding domain of human snRNP import factor snurportin 1
Full-length SPN1 did not crystallize, so fragments with m3G cap binding activity were generated by protease cleavage. The crystals of a 26 kDa fragment which were obtained had to be enlarged by two rounds of microseeding.
Acta Cryst. (2004). D60, 1632-1635
doi: 10.1107/S0907444904015872
doi: 10.1107/S0907444904015872
Open access
Biochemical, biophysical and preliminary X-ray crystallographic analyses of the fusion core of Sendai virus F protein
The Sendai virus fusion-core six-helix bundle assembly was crystallized; crystals diffract X-rays to 2.5 Å resolution.
Acta Cryst. (2004). D60, 1636-1638
doi: 10.1107/S0907444904017627
doi: 10.1107/S0907444904017627
Crystallization and preliminary X-ray analyses of desulfurization enzyme DszB and its C27S mutant complexed with biphenyl-2-sulfinic acid
Crystallization and preliminary X-ray analyses of desulfurization enzyme DszB were conducted.
Acta Cryst. (2004). D60, 1639-1640
doi: 10.1107/S0907444904016245
doi: 10.1107/S0907444904016245
Crystallization and preliminary X-ray diffraction analysis of HeLp, a heme lipoprotein from the hemolymph of the cattle tick Boophilus microplus
The main protein from the hemolymph of the cattle tick B. microplus has been crystallized using 1,6-hexanediol. The crystals belonged to the triclinic space group P1 and diffracted up to 2.1 Å resolution.
Acta Cryst. (2004). D60, 1641-1643
doi: 10.1107/S0907444904016403
doi: 10.1107/S0907444904016403
Crystallization and preliminary X-ray analysis of Escherichia coli MutT in binary and ternary complex forms
E. coli MutT was crystallized in binary and ternary complex forms. The crystals diffracted to 1.96 and 2.56 Å resolution, respectively.
Acta Cryst. (2004). D60, 1644-1646
doi: 10.1107/S0907444904016695
doi: 10.1107/S0907444904016695
The crystallization and preliminary X-ray analysis of heparinase II from P. heparinus is reported. Heparinases from P. heparinus are widely used in medical and analytical applications; however, their three-dimensional structures and detailed catalytic mechanisms have yet to be determined.
Acta Cryst. (2004). D60, 1647-1650
doi: 10.1107/S0907444904015185
doi: 10.1107/S0907444904015185
Purification, crystallization and preliminary X-ray analysis of Triatoma virus (TrV) from Triatoma infestans
Triatoma virus (TrV) is a viral pathogen of blood-sucking reduviid bugs (triatomines). This work describes the purification of TrV particles from infected T. infestans and their crystallization and preliminary crystallographic analyses.
Acta Cryst. (2004). D60, 1651-1653
doi: 10.1107/S0907444904016701
doi: 10.1107/S0907444904016701
Human coactosin-like protein has been expressed in high yield and the His-tagged protein was purified by affinity chromatography. Several different crystal forms were obtained by the hanging-drop vapour-diffusion method.
Acta Cryst. (2004). D60, 1654-1657
doi: 10.1107/S0907444904016841
doi: 10.1107/S0907444904016841
Open access
Crystallization and preliminary crystallographic studies of the cofactor-binding domain of the LysR-type transcriptional regulator Cbl from Escherichia coli
The cofactor-binding domain of the LysR-type transcriptional regulator Cbl from E. coli has been cloned, overexpressed, purified and crystallized. The crystals diffract to 3.0 Å resolution using synchrotron radiation.
Acta Cryst. (2004). D60, 1658-1661
doi: 10.1107/S0907444904016968
doi: 10.1107/S0907444904016968
Open access
Three different crystal forms of apXPF were grown in trigonal, monoclinic and triclinic systems. The trigonal crystals diffracted to 2.8 Å and were grown in the presence of double-stranded DNA. Monoclinic crystals were grown without DNA and diffracted to 3.2 Å. Triclinic crystals were grown from a truncated apXPF protein lacking the tandem helix–hairpin–helix motifs and diffracted to 2.1 Å.
Acta Cryst. (2004). D60, 1662-1664
doi: 10.1107/S0907444904017329
doi: 10.1107/S0907444904017329
Cloning, expression, purification, crystallization and preliminary X-ray crystallographic investigations of a unique editing domain from archaebacteria
The N-terminal module of an archaebacterial threonyl-tRNA synthetase responsible for editing function has been crystallized in P31(2)21 space group.
Acta Cryst. (2004). D60, 1665-1667
doi: 10.1107/S0907444904017561
doi: 10.1107/S0907444904017561
Open access
Expression, purification, crystallization and preliminary X-ray diffraction analysis of conjugated bile salt hydrolase from Bifidobacterium longum
Two different crystal forms have been obtained of the conjugated bile salt hydrolase from B. longum and their preliminary crystallographic characterization has been carried out.
Acta Cryst. (2004). D60, 1668-1669
doi: 10.1107/S0907444904016051
doi: 10.1107/S0907444904016051
X29, a nuclear Nudix hydrolase that binds and decaps U8 snoRNA, has been expressed in high yield and crystallized in a form that diffracts to 2.1 Å resolution.
short communications
Acta Cryst. (2004). D60, 1670-1673
doi: 10.1107/S0907444904010972
doi: 10.1107/S0907444904010972
Optimum solubility (OS) screening: an efficient method to optimize buffer conditions for homogeneity and crystallization of proteins
A solubility screen for determining the best solvent condition for a particular protein prior to crystallization trials is presented.
Acta Cryst. (2004). D60, 1674-1678
doi: 10.1107/S090744490401844X
doi: 10.1107/S090744490401844X
A preliminary solubility screen used to improve crystallization trials: crystallization and preliminary X-ray structure determination of Aeropyrum pernix flap endonuclease-1
A solubility screen has been utilized to optimize initial crystallization results of A. pernix flap endonuclease-1.
Acta Cryst. (2004). D60, 1679-1682
doi: 10.1107/S0907444904015215
doi: 10.1107/S0907444904015215
APRV is a program that automates routine procedures in high-throughput protein–ligand complex structure determination.
Acta Cryst. (2004). D60, 1683-1686
doi: 10.1107/S0907444904015835
doi: 10.1107/S0907444904015835
An algorithm for predicting the most informative wavelength combination in the MAD case is described.
Acta Cryst. (2004). D60, 1687-1690
doi: 10.1107/S0907444904015896
doi: 10.1107/S0907444904015896
The X-ray crystal structure of D-ribulose 5-phosphate 3-epimerase (RPE) from Synechocystis reveals that the enzyme shares a highly conserved active site with other RPEs.
PDB reference: D-ribulose 5-phosphate 3-epimerase, 1tqj, r1tqjsf
Acta Cryst. (2004). D60, 1691-1693
doi: 10.1107/S0907444904017615
doi: 10.1107/S0907444904017615
CLIMS is a laboratory-information management system for protein crystallography that features a novel graphical interface to a relational database and assists all aspects of protein crystallization projects.
addenda and errata
Acta Cryst. (2004). D60, 1694
doi: 10.1107/S0907444904019134
doi: 10.1107/S0907444904019134
Two orthorhombic crystal structures of a galactose-specific lectin from Artocarpus hirsuta in complex with methyl-α-D-galactose. Addendum
Addendum to the paper by Rao et al. (2004), Acta Cryst. D60, 1404–1412.


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View Single Post
18 Jan 2010
Capt.Jack Sparrow
Windows 7 Ultimate - 64-bit | Windows 8 Pro - 64-bit
4,979 posts
Quote: Originally Posted by
I used to use the
Fingerprint Reader to
login into my account, after installing Win7 (from Vista), finger-swipping is not working or not found as a device.
Biometric Devices: Authen Tec Inc. AE1610
Hi dinomon, Welcome to SF,
Have you install proper drivers for your Finger Print Reader device. If not go to their website. Download the Lastest drives for Windows 7 then install it. See whether it works or not.
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Hope this helps,
My System Specs
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Samsung NP550P5C-S02IN
Windows 7 Ultimate - 64-bit | Windows 8 Pro - 64-bit
Intel® Core i7 Processor 3,610QM (2.30Hz, 6MB L3 Cach
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1TB S-ATA II Hard Drive (5,400RPM)
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Baby Signing
3 Signs to Teach Babies First
The 3 Signs to Teach Babies First… Sometimes we wish our babies would come to us with full vocabularies and understanding enough of the world to know how to express their needs and wants. Obviously, it doesn't work that way and
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Warren Buffett has called hedge funds "manager compensation schemes." According to a recent article in The Economist, "Rich Managers, Poor Clients," investors pay for manager expertise, but controlling costs is a better way of having a successful investment than betting on a manager's track record. Some hedge funds charge performance fees of 20% of the gains plus another fixed 2-3% management fee regardless of whether the fund is profitable - a total of 22% in fees. In other words, the hedge fund would have to gain over 22% for investors just to breakeven. The only way to achieve those types of returns is to take extraordinary risk with your investment capital. As The Economist article stated: "It is easy to think of people who have become billionaire's by managing these hedge funds; it is far harder to think of any of their clients who have got as rich."
It is not only the costs associated with hedge funds that trouble me. The reality is they have performed poorly over the last 10 years, while they have made hedge fund managers very wealthy. As measured by the HFRX Indices (widely used benchmarks for hedge fund performance), hedge funds returned just 3% in 2012 compared to an 18% return of the S & P 500 stock Index. The major problem all hedge funds suffer these days is that they have attracted so much money that they cannot invest as they did in the past. There are too many dollars chasing too few market opportunities. And now, hedge funds are "going retail" and becoming widely available to small investors in Funds of Funds and becoming embedded in variable annuities, pension funds, endowments, foundations and other retirement plans. There are nearly 8000 hedge funds on the market and more coming online every day.
The biggest problem we have with hedge funds is a lack of transparency and the lack of due diligence that many brokers perform prior to offering and selling these investments to their biggest and best clients. The complexity and lack of transparency of hedge funds makes them the vehicle of choice for fraudsters. The United States Securities and Exchange Commission (SEC) has become especially concerned and set up a special unit "The Market Abuse Unit" to investigate the problems and abuses in the hedge fund industry. The SEC and the attorneys at our law firm are concerned about unregistered investment advisers engaging in general solicitations to unaccredited investors to put their capital in unregistered hedge funds. Too many retired, income-oriented investors, in search of higher yields have been misled into a number of hedge fund frauds such as the MAT/ASTA funds offered by Smith Barney and Citibank advisers.
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Economic impact growing
The numbers continue to be staggering:?Minot Air Force Base added $583.9 million into the local economy in fiscal year 2013, the largest economic impact in the base's history.
The past few years have seen some fluctuating figures, but have also continued to display a general growth pattern:
2008: $382.8 million
2009: $474 million
2010: $513.7 million
2011: $493.3 million
2012: $522.6 million
The annual report, presented this past week at the Minot Area Chamber of Commerce's Military Affairs Committee meeting, is yet another strong reminder of the important role played by Minot Air Force Base in the economy of Minot and the surrounding area. The annual payroll for military members, federal civilians and other civilians in fiscal year 2013 was more than $363 million.
Much of that money is, of course, spent in local businesses, from restaurants to gas stations to hotels to clothing stores to hardware stores to grocery stores. The impact of the base and its personnel is evident in every section of Minot's economy, and we expect the close relationship between the city and the base to continue well into the future.


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Fairfield Inn & Suites Houma: Find photos, reviews, information, amenities and compare prices for 3 stars hotels in Houma, United States.
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This 3-star hotel offers a BBQ/picnic area, a concierge and a safe. A fitness centre is available to guests wanting to keep active while travelling.
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TripAdvisor Reviews Hotel Bristol Vasto
Travel Blogs from Vasto
Day 52 - Taking a Break from Touring
... weather was a perfect 65 and sunny. While our scores were nothing to write home about, the round of golf itself was great.
We we left the golf course about 2:30 for Termoli, a small Adriatic coastal town near the spur of the boot of Italy. We found the hotel with only a minimum navigational delay. When we arrived, the wind was once again howling outside. We are wondering if this is not the norm for the Adriactic coast in the fall. We checked in about five and ...
From Coast to Coast
With a long drive ahead of us we got up reasonably early so the day wasn't taken completely with driving. Happy Fathers Day Kerry and Dad. Kerry got good wishes from all his children which made his day. It was going to take us four or so hours to drive to the other coast and we actually had a pretty clear run through mainly I think because its Sunday. No toll roads were taken but it was still good driving, not ...
Day 60- Ortona
... down on Positano before heading back through the wiggly roads through the mountains. After driving back through Naples we drve right accross Italy to a farmhouse in a olive orchard where we were staying the night. We visited a seafood shop on the fishing wharf and Dad bought oysters and mussels and scallops which mum cooked up for dinner. The farmhouse was a little rough but we spent the night planning some of the other destinations to follow. Good Night for now. - Grace
At Petacciato and Guglionesi
Some things don't change! The marina space at Petacciato is on of them. We had swum at this beach many years ago when we were house hunting at Carunchio, in the mountains close to the coast of southern Abruzzo. Clean sand, clear water and plenty of space before the Italian Summer ...
... after the match they picked me up. On the way to their house we stopped in at a food and drink shop, which Molly described as a bit like McDonald's except with booze and cheap food. And it literally was. Beers were in taps in place of soft drink and little sandwiches and olive bowls filled the back kitchen. Almost everything, including pints of beer, for €1.
Today, Alex picked me up at 11 and we head into the city for a coffee and croissant. She tells me that in ...
Other places to stay in Vasto
Dakar Living HotelStrada Statale 16 Sud n. 140, Vasto | 4 star hotelfrom $85
Hotel Principe residenceViale Dalmazia 124/126, Vasto | 3 star hotelfrom $86
Hotel ExcelsiorStrada Statale 16 Sud 266, Vasto | 4 star hotelfrom $141
Palace Hotel VastoS.S. 16, Vasto | Hotelfrom $149
Hotel RoyalViale Dalmazia 132, Vasto | Hotelfrom $92
Hotel SabrinaC. da San Tommaso, Vasto | Hotelfrom $64
Hotel CaravelViale Dalmazia 130, Vasto | Hotelfrom $97
Villa Vignola HotelLocalita Vignola S.S. 16, Vasto | Hotelfrom $164
Hampton Inn Sulphur210 Henning Drive, Sulphur | 2 star hotelfrom $90
Park Hotel ResortViale Dalmazia 165, Vasto | Hotelfrom $90
Hotel PresidentViale Dalmazia 136, Vasto | Hotelfrom $104
Locanda dei BaroniVia San Francesco D'Assisi 68/70, Vasto | Hotelfrom $83
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Thursday, November 3rd 11:58 AM IST
# Hajj # fake gold # gold prices # gold jewelry
According to analysts, increase in fake gold sale in Saudi markets especially during Hajj is a real problem for authorities as it will tarnish country's image.
RIYADH(BullionStreet): Gold markets in Saudi Arabia is in full swing at the moment as the annual Hajj pilgrimage is going on with millions of people from across the globe wants to buy at least a gram of gold from the holy land.
The sale this year is also reported to have climbed to new record so far but a new problem seems to have weakened the sales during the last few weeks.
Demand for gold was up around 10 per cent during hajj but high prices and fake gold threat might cut down sales this time, analysts warned.
According to analysts, increase in fake gold sale in Saudi markets especially during Hajj is a real problem for authorities as it will tarnish country's image.
Fake gold and jewelery are sold to pilgrims even by reputed gold shops in the kingdom as most of the pilgrims were unaware of the quality of the product they'r buying. On an average each pilgrim bought between 80 or 100 grams of gold during Hajj.
It is estimated that fraud cases in the Saudi gold market reached 35 percent during normal days, but rose up to 50 percent during Haj.
Many of the pilgrims were cheated after traders convince them that they were buying pure gold.
An Indian pilgrim who was cheated said he never believed that such a fraud will happen to him in the holy land, which is also known for its strict law enforcement. Indian jewelery set are fraudsters No 1 choice as pilgrims like them very much.
They said jewelers are taking advantage of unaware pilgrims who cannot even distinguish an 18 karat gold with a 22 karat one and never heard about the hallmarking of gold.
The most used trick is to sell gold mixed with zicron by which the jewlers can earn a handsome of money while customers were cheated by about $100 for evry ten grams of gold.
This percentage cannot be measured when the purchase was made and that's why traders infuse a large quantity of zircon and customers are not aware of the quantity in a particular item, analysts added.
Meanwhile, Saudi authorities along with gold traders and jewelers have decided to take strict measures against those involved in selling fake gold and jewelry.
As a first step, an agreement banning the exchange of damaged gold jewelry among wholesalers and factory owners without testing it or receiving payment with pure gold will be implemented soon.
The Saudi gold market consists of about 6,000 shops, and 250 manufacturing workshops that include 30 factories, in addition to 700 repair workshops.
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Finn, Harrison T.; Brennan, Scott L.; Gonano, Benjamin M.; Knox, Michael F.; Ryan, Rhearne C.; Siegler, Jason C.; Marshall, Paul W.M.
Abstract: Finn, HT, Brennan, SL, Gonano, BM, Knox, MF, Ryan, RC, Siegler, JC, and Marshall, PWM. Muscle activation does not increase after a fatigue plateau is reached during 8 sets of resistance exercise in trained individuals. J Strength Cond Res 28(5): 1226–1234, 2014—The premise of eliciting the greatest acute fatigue is accepted and used for designing programs that include excessive, potentially dangerous volumes of high-intensity resistance exercise. There is no evidence examining acute fatigue and neuromuscular responses throughout multiple sets of moderate-to-high intensity resistance exercise. Fifteen resistance-trained male subjects performed a single exercise session using 8 sets of Bulgarian split squats performed at 75% maximal force output. Maximal force output (N) was measured after every set of repetitions. Electromyographic (EMG) activity of vastus lateralis was monitored during all force trials and exercise repetitions. Repetitions per set decreased from the first to the third set (p < 0.001). Maximal force output decreased from preexercise to set 4 (p < 0.001). Electromyographic amplitudes during exercise did not change. Secondary subgroup analysis was performed based on the presence, or not, of a fatigue plateau (<5% reductions in maximal force output in subsequent sets). Nine participants exhibited a fatigue plateau, and 6 did not. Participants who plateaued performed less first-set repetitions, accrued less total volume, and did not exhibit increases in EMG amplitudes during exercise. Initial strength levels and neuromuscular demand of the exercise was the same between the subgroups. These data suggest that there are individual differences in the training session responses when prescribing based off a percentage of maximal strength. When plateaus in fatigue and repetitions per set are reached, subsequent sets are not likely to induce greater fatigue and muscle activation. High-volume resistance exercise should be carefully prescribed on an individual basis, with intrasession technique and training responsiveness continually monitored.
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Get an overview of this hotel3-star bed & breakfast with free breakfast and restaurant
Collect 400 Nectar points!
Simply enter your Nectar card number when you check out. Your points will be credited 35 days after you return from your trip.*Offers may apply
Situated in the business district, this bed & breakfast is within 6 mi (10 km) of Jamestown Lighthouse, Holy Trinity Cathedral, and Kwame Nkrumah Memorial. Makola Market and Centre for National Culture are also within 6 mi (10 km).
Property Features
A restaurant, an outdoor pool, and a bar/lounge are available at this bed & breakfast. Free continental breakfast, free WiFi in public areas, and free self parking are also provided. Other amenities include a poolside bar, a meeting room, and laundry facilities.
Room Amenities
All 20 rooms offer free WiFi, refrigerators, and flat-screen TVs with satellite channels. Minibars, room service, and phones are among the other amenities available to guests.Information missing or incorrect? Tell us!Opens in a new window
Forest Gate Hotel
Bed & Breakfast Amenities
Bed & Breakfast Amenities
The bed & breakfast offers a restaurant. Guests can unwind with a drink at one of the bed & breakfast's bars, which include a poolside bar and a bar/lounge. Guests can enjoy a complimentary breakfast. Public areas are equipped with complimentary high-speed wireless Internet access.
This Accra bed & breakfast also offers an outdoor pool, tour/ticket assistance, and multilingual staff. For a surcharge, a shuttle from the airport to the hotel (available on request) is offered to guests. Onsite self parking is complimentary.
- Luggage storage
- Free breakfast
- Safe-deposit box at front desk
- One meeting room
- 24-hour front desk
- Tours/ticket assistance
- Total number of rooms - 20
- Number of floors - 2
- Airport transportation - pickup (surcharge)
- Bar/lounge
- Free newspapers in lobby
- Free WiFi
- Laundry facilities
- Multilingual staff
- Garden
- Poolside bar
- Restaurant
- Free self parking
- Outdoor pool
Family Friendly Amenities
- Family Hotel
- Outdoor pool
- Free Wi-Fi
- Laundry facilities
- Refrigerator
Available in all rooms: Free WiFi
Available in some public areas: Free WiFi
Free self parking
Room Amenities
- Desk
- Satellite TV service
- Flat-panel TV
- Refrigerator
- Air conditioning
- Private bathroom
- Phone
- Minibar
- Daily housekeeping
- Free toiletries
- Shower/tub combination
- Free WiFi
- Room service (limited hours)
Where to Eat
Guests are offered a complimentary continental breakfast.
Forest Gate Hotel has a restaurant on site.
Room service (during limited hours) is available.
Nearby Things to Do
Recreational amenities at the bed & breakfast include an outdoor pool.
If you have requests for specific accessibility needs, please note them at check-out when you book your room.
Bed & Breakfast Policies
Check-in time starts at noon
Check-in time ends at 6 PM
Special check-in instructions:
Check-out time is 11 AM
Payment types
Children and extra beds
- Children are welcome.
- Kids stay free! Children 12 years old and younger stay free when using existing bedding.
- No pets or service animals allowed
You need to know
Extra-person charges may apply and vary depending on property policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
No rollaway/extra beds available
No cribs (infant beds) available
No pets or service animals allowed
Optional extras
- Airport shuttle fee: GHS 20.00 per vehicle
Bed & Breakfast Name
- Forest Gate Accra
- Forest Gate Hotel
- Forest Gate Hotel Accra
- Hotel Forest Gate
We should mention
Children 12 years old and younger stay free when occupying the parent or guardian's room, using existing bedding. No pets and no service animals are allowed at this property.


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Rates: Air and Land Prices may change due to currency fluctuations, changes in the tax, or changes in the fuel surcharges.Reservations and Payments: A completed tour application together with a deposit of $400.00 per person must be received before confirming your reservations. Final payment must be received by March 1, 2017. Payments received after March 1 are subject to a $50 per person late fee.Cancellations: A $400 per person handling fee will be made if cancellation is received in writing 90 days or more prior to departure. A $600 per person cancellation fee will be charged if received in writing 61 to 90 days prior to departure. Cancellations made in writing within 60 days prior to departure are subject to a $600 per person charge plus any nonrecoverable land package charges. Airline tickets once purchased are non-refundable but may be used for future travel on that airline within one year.Not included in price: All items not specifically listed under tour features. Visa fees are additional. Insurance: For your protection, trip cancellation, baggage, health and accident insurance is highly recommended for all participants. However, no representation or description of the insurance made by our staff constitutes a binding assurance or promise about the insurance.
Responsibility: These tours are operated by Marathon Tours, Inc. of Boston, MA who acts only as an agent for the passenger to the companies offering such services and assumes no liability for injury, damage, loss, accident, delay or irregularity caused by any reason whatsoever by any party offering such services. The right is reserved to cancel the tour prior to departure with a full refund made as full settlement to the passenger. Marathon Tours, Inc. may make any changes or alterations in the itinerary as it deems necessary for the proper handling of the tour and may refuse any person as a member of the tour. The sole responsibility of any airline used is limited to that set out in the passenger contract evidenced by the ticket. The airlines concerned are not to be held responsible for any act, omission or event during the time that the passengers are not on board their aircraft.


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Arbor House Inn
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Day 29 - Stop-over
... Given that description, we weren't expecting much - it is just a stop on the road west - but it is a sweet and quaint little place with a lot of potential. Unfortunately, it just seems tired and forgotten. The motel we are staying in is basic and functional, yet has the most stunning views across the lake to the back. There is even a little chapel (visualise a nicely painted shed) in the garden for weddings. The owner welcomed us with the type of enthusiasm ...
Lake county
... seen before. It's a British crime drama with Edris Elba (who I confessed until recently I used to think was an American actor as I had only seen him as Stringer Bell in The Wire) Like a lot of other British black actors there is more work for available in America than the UK so it's not uncommon for them to work over here instead. Tomorrow I'm going out with Terry to a timebank members house to help pick ...
Final Pick!
We're up at first light and down to the vines for the back breaking task of the last pick of the year! Armed with water, sun cream and shears we work our way through the rows of vines, slowly filling the bins.. Today is volunteer day and with the promise of a magnum of wine for their trouble, Paul ...
Redwoods & North California
... is the last town before/after the 'lost coast'.
The next days we stayed in Fort Bragg and did a day excursion to Mendocino. What a beautiful little town on the coast. Many houses were build in Victorian style and other individualistic styles.
It is a unique place and worth staying. Though it seemed very quite and after 5pm there is probably no one on the street and most cafes will have closed. People who like art will find a lot ...
A treasure hunt for mushrooms
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August 2, 2013
HELENA (AP) — Strong thunderstorms that moved across Montana left a trail of damage to crops and buildings along with downed trees and power outages.
The worst damage from Thursday's storm may have occurred in Gallatin County, where winds gusting up to 89 mph devastated wheat and barley crops that likely would have been harvested next week, Montana Grain Growers Vice President Matt Flikkema said.
"I've never seen crop damage to the extent we have here in the valley," Flikkema said Friday. "There are very little crops that will be harvested out of the area."
Flikkema said the damage co...
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Would you like to save as your currency permanently or for this visit only?
Hyatt Regency Dallas At Reunion
Top City Hotel
300 Reunion Boulevard, Dallas, TX
0.1 km from
Vibe was good
Generally very clean
Great service
Friendly staff, beautiful location and near everything. PARKING
Everything else. Overpriced compared to facility. The room could have been cleaner. The entrance location is a bit tricky for pedestrians. Not my best hotel experience.
View was awesome! Staff was really nice and helpful. Definitely will be stay here again when the chance comes up Nothing was wrong
The room was clean enough. Valet is a joke. Staff is rude. Not pet friendly at all. Terrible location, hard to access. Expect long waits for any service needed and fees that aren't shown online. Make sure to really check your bill upon leaving. Ours was screwy. Couldn't complete a quick check-out, because we had to go to the desk to get it fixed, which took forever.
Very nice hotel, with excellent meeting facilities. Very convenient to West End nightlife and dining. Good food. Easy and cheap access to airport via rail (DART or TRE). The train is a little noisy, even on the opposite site of the building.
My entire experience was weird. When we first arrived, we wanted to unload our car and I got yelled at like I was a child. Literally yelled at. I had to be firm back in order to get direction as to what te he'll I was supposed to do. Then, upon check in, I was informed that for our two cars, self park was $40. I had no idea it would be so much. I was extremely unhappy but tried to remain optimistic. I purposefully found a hotel with a Pool per request of our group. The pool is a sick hilarious joke. It's the size of a van. It was ridiculous. It was also completely full because of the 10 whole people who were in it. Left the hotel an figured out they overcharged me. Called them and they "took care of it". Well they isn't it still is not reconciled in my account. Over charged, expensive parking, rude parking dude, misleading pool size.
All good
I loved the location. Friday nights dinner at five-sixty was wonder full and the view amazing. Saturday morning we awoke and took a stroll through downtown dallas. And the shops on the 1st level were convenient. So last weekends stay was all for fiancee....Shes been really stressed lately and i wanted to give her a night to remember.First off partially my fault, but i booked on prom weekend so parking was horrible. I actually ended up parking across the street from Reunion Tower. Then greeted at the front desk i find that they've stuck me into a double queen handicap room, not ok. After speaking with the young lady down stairs she apologizes and gives me a different room. Again its a double queen not the single room. I had booked. Then when i go to wash up before dinner at the five-sixty, the drain to the sink was clogged and water poured on to the floor. Kids at the prom were having fun on the balcony, they were laughing, screaming and enjoying theyre night. Unfortunately for us, we were located right above...
A remarkable hotel at the epicenter of Dallas. The hotel has immediate access to all local Dart rail trains (leading everywhere), the T (taking you to Fort Worth) and Amtrak with straight connections to Chicago and Los Angeles. A memorable vacation starts with 1 or 2 nights here and then a surprise trip either on Amtrak or Dart out to DFW airport. The atrium is amazing with views of Reunion tower and the famed 560 Wolfgang Puck's restaurant. Make your reservations at least 2-3 weeks out to get an available time slot for a memorable night viewing downtown Dallas from 3/4 of mile away and 560 feet up in the air. Room was spacious and immaculate and view of downtown, rail station, Omni was incredible. Request a view of downtown when you check in. Valet parking is a little pricey so I opted for self pay parking at the Amtrak station ($6 for 12 hours)
I absolutely loved it. We stayed at the Hyatt for my boyfriends 30th birthday and the staff was phenomenal. At check in they asked us what the occasion was and upon finding out they sent us a bottle of wine and appetizers to our room. the first night we ate at the little Italian bistro and not only was it delicious but the waiter Chris was so nice. Natasha in the bar area was equally amazing. We had a wonderful view of the city on the 25th floor. I have stayed at several other locations in the city.. Zaza, Magnolia, Joules etc. this is the best bang for your buck. Thank you so much for assisting in making it a special weekend!! We self parked, and the walk from self parking is pretty long we got lost in the hotel trying to find our way to the front desk. (we parked in the parking lot closest to reunion tower)
Contemporary rooms with great staff. Highly satisfied.
Super pet friendly. They welcomed my dog with a hyatt dog bed and dog dish for her and also gave us two treats that she enjoyed. However, there is a pet depost of $100 that's non-refundable
proximity of downtown, personnal are very gentle, good service
It was interesting to stay at an older renovated hotel. The improvements- showers especially-were very good. The view was great of the Old Red Court House and in walking distance of the West End restaurant area. It was also right across from Union Station with the Dart and tre trains. With those and the hotel shuttle bus you could get anywhere in town. It was nice to have breakfast included. Our bathroom was a bit tight but manageable.
The stay was comfortable for two guest, liked that Hotel had a resturant in the stay. Did not like the parking area of the Hotel!
Great location Parking is not included
the closeness of all the sights we wished to see in the time we had there
I enjoyed the staff. I found them personable, helpful, and willing to go out of their way for my needs. The breakfast was okay. It seems as the week went on, the food seemed to be recycled. The biscuits and gravy became more water downed as the week progressed.


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These latest diaries show a man grasping every opportunity that came his way, and they deal candidly with the doubts and setbacks that accompany this prodigious work-rate. As ever, his family life, with three children growing up fast, is there to anchor him.
Travelling to Work is a roller-coaster ride driven by the Palin hallmarks of curiosity and sense of adventure. These ten years in different directions offer riches on every page to his ever-growing army of readers.


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In the novel A Lesson Before Dying although Grant is an educated black man in the era of a racist society he has struggles greater than most men of his decent I feel sorry for him because of his limitations even though I view him as a coward He cannot break free of his background and family The three main female characters in the novel Tante Lou Miss Emma and Vivian restrict and limit Grants choices Grant realizes that freedom means leaving his small town and creating a new life yet each woman holds a chain that keeps him from his destiny and the right to be freeFirst Tante Lou his aunt holds Grant from his dreams by refusing to let him go his own way Tante Lou wants Grant to stay at home with her and take care of her But in the time the novel takes place it wasnt common for young men stay with their elders and help out especially when Grant has a college degree and can accomplish so much Tante Lou took advantage of this in any means necessary using the fact that Grant is family and in essence owes it to her to stay and help out We can see this on page 14 when Tante Lou forces Grant to talk to Mr Henri about seeing Jefferson You are going up there with us Grant or you will not sleep in this house tonight Tante Lou denies Grant choices that men his age have Next Miss Emma who doesnt have a family relation to Grant also restricts his choices in life Miss Emma plays an important role in the novel by being Jeffersons godmother and in a painstaking situation She asks Grant in a way that gives him no real choices to teach Jefferson to be a man Leaning on her ability to persuade and using her power as an extensive friend to Tante Lou she can basically
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Could Nicole Scherzinger be too old for Radio 1? Well apparently it seems she's "not right" for their youthful target audience.
For the second time in just over a year, the Beeb have left 36-year-old Nicole off their playlist.
And for the second time, the single is still riding high in the charts regardless.
Last March, Nicole's Boomerang failed to receive support from both Radio 1 and Capital FM.
But the latter adjusted their playlist to accommodate it once it charted high in the midweeks. Radio 1 still left it off theirs.
This week, Your Love is battling with Rixton for the top spot in Sunday's Official Charts – but while the boyband, who are in their mid-twenties, are getting maximum support on the station's A List, Nicole hasn't even scraped on to the C list. The boys are currently 12,000 copies ahead in the race for No 1.
In a statement, Radio 1 say: "Each track is considered for the playlist based on its musical merit and whether it is right for our target audience, with decisions made on a case-by-case basis."
My sources say: "Nicole's single was discussed at the playlist meeting, but it wasn't considered a priority track.
"It's not personal against her - she has been a guest on Radio 1 and the track has been played – just not playlisted.
"Radio 1 plays a much wider range of artists than commercial radio – they largely concentrate on playing hits."
Previously, the station has been criticised for deeming artists including Robbie Williams
"not relevant" due to their age, which is above the station's demographic.
The station targets the 15-24 age group, and Robbie's single, Candy, failed to achieve playlist status when he released it at the age of 38.
The current A List includes Ariana Grande, 21, George Ezra, 21, Ella Eyre, 20, and teen boyband The Vamps.


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- 01-09-2014, 03:14 PM Thread Author #1
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Recommended to enter Google account even if you don't install any apps?
For a new AT&T SG3 mini does one (or rather should one) enter a Google account to make sure your phone gets updates to the apps that were pre-installed, like Chrome browser? My Dad doesn't have a Google Account, and he will only use the apps that are pre-installed, but should I still set up a Google Account and log him in, or will AT&T's regular updates be enough because he'll never install one single app. The only apps he'll use are the pre-installed android email client, chrome browser, and native SMS texting. Weather widgets too.
Side note--- I guess I have another question. I'm a bit confused because there is also a Samsung App store. Can that be removed, should it be, or will the pre-installed apps be updated through Samsung's App store?
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