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Department: Department of Biological Sciences
Degree Discipline: Biology
Collection: UNT Theses and Dissertations
- Acute Effects of the Antibiotic Streptomycin on Neural Network Activity and Pharmacological Responses
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The purpose of this study is to find out that if antibiotic streptomycin decreases neuronal network activity or affects the pharmacological responses. The experiments in this study were conducted via MEA (multi-electrode array) technology which records neuronal activity from devices that have multiple small electrodes, serve as neural interfaces connecting neurons to electronic circuitry. The result of this study shows that streptomycin lowered the spike production of neuronal network, and also, sensitization was seen when neuronal network pre-exposed to streptomycin. digital.library.unt.edu/ark:/67531/metadc700026/
- The Adolescent Stress Response to a Naturalistic Driving Stressor
- The proposed study examined the role of anxiety and risk-taking in driving performance in adolescents. In addition to examining the sample as a whole, gender differences were assessed given earlier reports from our laboratory and others indicating that males and females differ with respect to risky behaviors to driving performance and anxiety. Adolescents' subjective and physiological responses to a driving simulator task were assessed. Anxiety was measured via self report and salivary cortisol. Participants provided a baseline saliva sample and 3 post-task samples for cortisol analysis. Subjective anxiety scores were obtained at both baseline and following the driving stressor. Information concerning impulsivity, as well as other psychological constructs was also collected at baseline. Unlike the pilot study, there were no relationships (with or without respect to gender) between salivary cortisol and both self-reported anxiety (state and trait) or impulsively measures for this sample. These results suggest that this group of adolescents may not have been anxious about the driving task. This discrepancy may stem from error introduced by the smaller sample size obtained from the initial findings or to other factors remaining outside the parameters of the current study. The task did, however, induce a slight hypothalamic pituitary adrenal (HPA) axis response indicating some physiological arousal. Males had significantly higher cortisol levels at baseline than females and at time point 3 while approaching significance at time points 2 and 4. Females possessed significantly higher trait anxiety than males and all post task cortisol levels were positively correlated to age while time points 2 and 4 (with time point 3 approaching significance, p=0.09) were inversely correlated with Self Depreciation scores. Additionally, females had Persecutory Ideas scores that were also negatively correlated with cortisol at time points 3 and 4. For both the entire sample and males only, the correlation between post-task cortisol and driving performance was positive and approached significance (p=0.07 and p=0.08, respectively), suggesting that some HPA activation may be facilitative for successful driving task performance. Correlations between driving performance and psychological constructs were explored and discussed with and without respect to gender. digital.library.unt.edu/ark:/67531/metadc2591/
- Age-Dependent Effects Of Chronic GABAA Receptor Blockade In Barrel Cortex
- GABAA receptor binding is transiently increased in rat whisker barrels during the second postnatal week, at a time when neurons in the developing rat cortex are vulnerable to excitotoxic effects. To test whether these GABAA receptors might serve to protect neurons from excessive excitatory input, polymer implants containing the GABAA receptor antagonist bicuculline were placed over barrel cortex for a 4-day period in young (postnatal days 8 - 12) and adult rats. In the cortex of young, but not adult rats, the chronic blockade of GABAA receptors resulted in substantial tissue loss and neuron loss. The greater loss of neurons in young rats supports the hypothesis that a high density of GABAA receptors protects neurons from excessive excitatory input during a sensitive period in development. digital.library.unt.edu/ark:/67531/metadc2760/
- Aging Is a Determinant in Anoxia Stress Tolerance in Caenorhabditis Elegans
- Oxygen availability is critical for survival for most organisms. The nematode, C. elegans, has been useful for studying genetic regulation of anoxia tolerance due to the oxygen deprivation response mechanisms shared with other metazoans. Studies examining long-term anoxia (72h, LTA) tolerance have only been conducted at adult day 1. To investigate the effect of aging on anoxia tolerance wild-type and mutant strains were exposed to LTA between adult day 1 and day 9. Wild-type isolates and daf-16(mu86) (FOXO transcription factor regulated by insulin-signaling) and aak-2(gt33) (catalytic subunit of AMP-activated protein kinase) strains were anoxia sensitive at day 1 and displayed increased LTA tolerance with aging correlated with reproductive senescence followed by a decline in survivorhsip through day 9. The daf-2(e1370) (insulin receptor homologue of C. elegans), glp-1(e2141) (a lin-12/Notch receptor) and fog-2(q71) (required for spermatogenesis) strains were LTA-tolerant through day 5. I conclude that aging influences LTA-tolerance in a strain- and age-dependent manner. In addition to being LTA-tolerant the daf-2(e1370) and glp-1(e2141) strains have a longevity phenotype that is suppressed by loss of kri-1 or daf-12. While loss of kri-1 did not suppress the LTA-tolerant phenotype of glp-1(e2141) at day 1 the portion of impaired survivors increased at day 3 and by day 5 tolerance was suppressed. Similarly, when exposed to 4 days of anoxia the glp-1(e2141);daf-12(rh41rh611) double mutant had a reduced survivor rate at all ages analyzed compared to glp-1(e2141) controls. To better understand formation of an anoxia-tolerant physiology I exposed adults to one or more 24h bouts. Recurrent bouts increased LTA tolerance in wild-type hermaphrodites in a dose-dependent manner. Bout-treated daf-16(mu86) animals had increased survival rate compared to controls yet maximum survival remained below age-matched wild-type. Anoxia bouts decreased LTA-tolerance in aak-2(gt33) mutants, indicating the requirement for ATP regulation in establishing an LTA-tolerant phenotype. These data support the idea that anoxia tolerance is multi-factorial and influenced by environment, metabolism, food, reproduction, sex phenotype and likely additional factors. digital.library.unt.edu/ark:/67531/metadc271821/
- Analysis of the Trypanosoma brucei Genome and Identification and Characterization of a Gene Family Encoding Putative EF-Hand Calcium-Binding Proteins
- The flagellum of Trypanosoma brucei contains a family of antigenically related EF-hand calcium-binding proteins which are called the calflagins. Genomic Southern blots indicated that multiple copies of calflagin genes occur in T brucei. All of the copies were contained in a single 23 kb Xhol-Xhol fragment. Genomic fragments of 2.5 and 1.7 kb were cloned that encoded calflagin sequences. Two new members of the calflagin family were found from genomic clone sequences. The deduced amino acid sequences of the genomic clones showed the calflagin genes were arranged tandemly along the genomic fragments and were similar to previously described calflagins. The calflagin genes were related by two unrelated 3' flanking sequences. An open reading frame that was unrelated to any calflagin was found at the 5' end of the 2.5 kb genomic fragment. Each encoded protein (~24,000u) contained three EF-hand calcium-binding motifs and one degenerate EF-hand motif. In general, variability among the T. brucei calflagins is greater than related proteins in T. lewisii and T. cruzi. This variability results from amino acid substitutions at the amino and carboxy termini, and duplication of internal segments. digital.library.unt.edu/ark:/67531/metadc278592/
- Anatomical and Morphological Responses of Cardiospermum Halicacabum L. (Balloon Vine), to Four Levels of Water Availability
- C. halicacabum (Sapindaceae) is an invasive plant that is considered a nuisance species in Texas riparian environments. Little is known of the tolerance of C. halicacabum to flooding and drought; however, this information may provide insight into the characteristics that contribute to C. halicacabum purported invasiveness. C. halicacabum seedlings (n = 92) were exposed to one of four levels of water availability (flooded, saturated, intermediate and dry) over six weeks under greenhouse conditions. Plant performance was affected by water availability; however, there was no effect on survivorship. Flooded and saturated plants exhibited morphological adaptations; producing adventitious roots, hypertrophy, and aerenchyma tissue. Morphological measures, anatomical responses, and patterns of biomass allocation all indicate that C. halicacabum is able to survive periodic inundation, perform in saturation, and establish and thrive on the drier end of a moisture gradient. digital.library.unt.edu/ark:/67531/metadc67974/
- Application of Cultured Neuronal Networks for Use as Biological Sensors in Water Toxicology and Lipid Signaling.
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This dissertation research explored the capabilities of neuronal networks grown on substrate integrated microelectrode arrays in vitro to be applied to toxicological research and lipid signaling. Chapter 1 details the effects of chlorine on neuronal network spontaneous electrical activity and pharmacological sensitivity. This study demonstrates that neuronal networks can maintain baseline spontaneous activity, and respond normally to pharmacological manipulations in the present of three times the chlorine present in drinking water. The findings suggest that neuronal networks may be used as biological sensors to monitor the quality of water and the presence of novel toxicants that cannot be detected by conventional sensors. Chapter 2 details the neuromodulatory effects of N-acylethanolamides (NAEs) on the spontaneous electrical activity of neuronal networks. NAEs are a group of lipids that can mimic the effects of marijuana and can be derived from a variety of plant sources including soy lecithin. The most prominent NAEs in soy lecithin, palmitoylethanolamide (PEA) and linoleoylethanolamide (LEA), were tested individually and were found to significantly inhibit neuronal spiking and bursting activity. These effects were potentiated by a mixture of NAEs as found in a HPLC enriched fraction from soy lecithin. Cannabinoid receptor-1 (CB1-R) antagonists and other cannabinoid pathway modulators indicated that the CB1-R was not directly involved in the effects of NAEs, but that enzymatic degradation and cellular uptake were more likely targets. The results demonstrate that neuronal networks may also be a viable platform for the elucidation of biochemical pathways and drug mechanisms of action. digital.library.unt.edu/ark:/67531/metadc5557/
- Applications of Remote Sensing and GIS to Modeling Fire for Vegetative Restoration in Northern Arizona
- An accurate fire model is a useful tool in predicting the behavior of a prescribed fire. Simulation of fire requires an extensive amount of data and can be accomplished best using GIS applications. This paper demonstrates integrative procedures of using of ArcGIS™, ERDAS Imagine™, GPS, and FARSITE© to predict prescribed fire behavior on the Kaibab-Paiute Reservation. ArcGIS was used to create a database incorporating all variables into a common spatial reference system and format for the FARSITE model. ArcGIS Spatial Analyst was then used to select optimal burn sites for simulation. Our predictions will be implemented in future interagency efforts towards vegetative restoration on the reservation. digital.library.unt.edu/ark:/67531/metadc4323/
- Aspartate Transcarbamoylase of Aeromonas Hydrophila
- This study focused on the enzyme, aspartate transcarbamoylase (ATCase) from A. hydrophila, a Gram-negative bacterium found in fresh water. The molecular mass of the ATCase holoenzyme from A. hydrophila is 310 kDa. The enzyme is likely composed of 6 catalytic polypeptides of 34 kDa each and 6 regulatory polypeptides of 17 kDa each. The velocity-substrate curve for A. hydrophila ATCase is sigmoidal for both aspartate and carbamoylphosphate. The Km for aspartate was the highest to date for an enteric bacterium at 97.18 mM. The Km for carbamoylphosphate was 1.18 mM. When heated to 60 ºC, the specific activity of the enzyme dropped by more than 50 %. When heated to 100 ºC, the enzyme showed no activity. The enzyme's activity was inhibited by ATP, CTP or UTP. digital.library.unt.edu/ark:/67531/metadc5840/
- An Assay Method for Determining Extra-Cellular Lipases from Pseudomonas aeruginosa
- The applicability of an isotopically labelled assay system to determine the lipase production in Pseudomonas aeruginosa was evaluated. Supernatant from cultures of Pseudomonas aeruginosa grown in a medium containing olive oil was incubated with a substrate containing labelled trioleate. Fatty acids were isolated by means of a liquid-liquid partition system. Enzyme activity was determined by measuring the amounts of free fatty acid by liquid scintillation counting. Findings indicate that the isotopicallylabelled, liquid-liquid partitioning assay is reliable, sensitive and adaptable to rapid assay conditions. It was also determined that different strains of Pseudomonas aeruginosa produce varying amounts of lipase. Partial purification of supernatant by gel filtration produced two protein peaks showing enzymatic activity. digital.library.unt.edu/ark:/67531/metadc504214/
- Assembly of Pseudomonas putida Aspartate Transcarbamoylase and Possible Roles of the PyrC' Polypeptide in the Folding of the Dodecameric Enzyme
- Aspartate transcarbamoylase (ATCase) of Pseudomonas putida consists of two different polypeptides, PyrB and PyrC' (Schurr et al, 1995). The role of the PyrC' and the assembly of PyrB and PyrC' have been studied. The ATCase made in vitro of P.putida PyrB with P.putida PyrC', and of E.coli PyrB with P.putida PyrC ' were generated under two different conditions, denaturation and renaturation, and untreated. It was found that PyrC' plays a role in the enzymatic regulation by ATP, CTP and UTP. In addition to playing a role in substrate binding, the PyrB polypeptide is also involved in effector binding (Kumar et al., manuscript in preparation). The most energetically preferred form of the P.putida WT is a dodecamer with a molecular mass of 480 kDa. The ratio between the PyrB and the PyrC' is 1:1. In studies of nucleotide binding, it was discovered that the P.putida PyrB was phosphorylated by a protein kinase in the cell extract. In the presence of 20 mM EDTA, this phosphorylation was inhibited and the inhibition could be overcome by the addition of divalent cations such as Zn2+ and Mg2+. This result suggested that the phosphorylation reaction required divalent cations. In the CAD complex of eukaryotes, phosphorylations of the CPSase and the linker region between ATCase and DHOase did not occur in the presence of UTP and it was hypothesized (Carrey, 1993) that UTP and phosphorylation(s) regulated the conformational change in the enzyme complex. Therefore, the same idea was approached with P.putida ATCase, where it was found that 1.0 mM UTP inhibited the phosphorylation of PyrB by more than 50%. These results suggested that the regulation of the conformational change of the P.putida ATCase might be similar to that of CAD. Furthermore, peptide mapping for phosphorylation sites was performed on P.putida ATCase WT, WT --11 amino acids and WT --34 amino acids from the N-terminus of the PyrB polypeptide. The results showed that the phosphorylation sites were located on the fragment that contained amino acid number-35 to amino acid number-112 from the N-terminus of the PyrB polypeptide. digital.library.unt.edu/ark:/67531/metadc278618/
- Assessing Effects of an Environmental Education Field Science Program Fostering Responsibility at an Urban Middle School
- The study investigated the ability of an extracurricular program to influence environmental responsibility of sixth and seventh graders. The Children's Environmental Attitude and Knowledge Survey (CHEAKS) was evaluated for appropriateness in assessing the worth of this particular environmental education strategy emphasizing water quality fieldwork and technology. CHEAKS is designed with psychometric reliability and validity that may be used in comparing disparate programs. Wilcoxon two sample tests were used to analyze data gathered from two student groups; one participated in an "Enviro-Mentals Club"; the other received no treatment. Analysis showed no significant change in environmental attitudes between groups, but did show significance (p <= 0.05) in environmental knowledge growth. Therefore, the investigated program had marginal success in influencing environmental responsibility. digital.library.unt.edu/ark:/67531/metadc277772/
- Attenuation of Escherichia Coli Aspartate Transcarbamoylase Expressed in Pseudomonas Aeruginosa Mutant and Wild Type Strains
- No apparent repression of pyr gene expression in Pseudomonas aeruginosa is observed upon addition of exogenous pyrimidines to the growth medium. Upon introduction of the subcloned Escherichia coli pyrBI genes for aspartate transcarbamoylase (ATCase) into a P. aeruginosa pyrB mutant strain, repression was observed in response to exogenously fed pyrimidine compounds. The results proved that it is possible to bring about changes in pyrimidine nucleotide pool levels and changes in transcriptional regulation of gene expression as a result. Thus, the lack of regulatory control in P. aeruginosa pyr gene expression is not due to an inability to take up and incorporate pyrimidine compounds into metabolic pools, or to an inability of the RNA polymerase to respond to regulatory sequences in the DNA but is probably due to a lack of specific regulatory signals in the promoter of the genes themselves. digital.library.unt.edu/ark:/67531/metadc279106/
- Automated Low-cost Instrument for Measuring Total Column Ozone
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Networks of ground-based and satellite borne instruments to measure ultraviolet (UV) sunlight and total column ozone have greatly contributed to an understanding of increased amounts of UV reaching the surface of the Earth caused by stratospheric ozone depletion. Increased UV radiation has important potential effects on human health, and agricultural and ecological systems. Observations from these networks make it possible to monitor total ozone decreases and to predict ozone recovery trends due to global efforts to curb the use of products releasing chemicals harmful to the ozone layer. Thus, continued and expanded global monitoring of ozone and UV is needed. However, existing automatic stratospheric ozone monitors are complex and expensive instruments. The main objective of this research was the development of a low-cost fully automated total column ozone monitoring instrument which, because of its affordability, will increase the number of instruments available for ground-based observations. The new instrument is based on a high-resolution fiber optic spectrometer, coupled with fiber optics that are precisely aimed by a pan and tilt positioning mechanism and with controlling programs written in commonly available software platforms which run on a personal computer. This project makes use of novel low-cost fiber optic spectrometer technology. A cost advantage is gained over available units by placing one end of the fiber outdoors to collect sunlight and convey it indoors, thereby allowing the spectrometer and computer to be placed in a controlled environment. This reduces the cost of weatherproofing and thermal compensation. Cost savings also result from a simplified sun targeting system, because only a small pan and tilt device is required to aim the lightweight fiber optic ends. Precision sun-targeting algorithms, optical filter selection, and software to derive ozone from spectral measurements by the spectrometer are a major contribution of this project. This system is a flexible platform which may be adapted to study other atmospheric constituents such as sulfur dioxide, nitrous oxides, and haze. digital.library.unt.edu/ark:/67531/metadc5792/
- Autonomic Reflexes of the Heart During Acute Myocardial Ischemia
- This study investigated whether acute myocardial ischemia of the anterior left ventricular wall induced an increase in cardiac sympathetic efferent nerve activity and thereby affected regional myocardial blood flow and contractile function. digital.library.unt.edu/ark:/67531/metadc279150/
- Autoradiographic Localization of Carbachol-Induced Second Messenger Response in the Rat Spinal Cord Following Inflammation.
- This study examined central mechanisms of persistent pain using an autoradiographic technique to localize phosphoinositide hydrolysis (PI) in the rat spinal cord dorsal horn. The lateral half of laminae I-II showed the highest levels of baseline PI turnover and carbachol-stimulated PI turnover in normal animals as well as after inflammation. Inflammation resulted in increased baseline PI turnover in this region of the ipsilateral (76%) and contralateral (65%) dorsal horns. Carbachol increased PI turnover in this region in normal rats (55%) and following inflammation (ipsilateral: 46%, contralateral: 45%). The absolute magnitudes of these increases were 1.85, 2.71, and 2.51 nCi/mg, respectively. The results of this study demonstrate the involvement of PI turnover in neural mechanisms of persistent pain, and provide evidence for the involvement of cholinergic systems in this process. Because spinal cholinergic systems have been reported to be anti-nociceptive, the present results appear to reflect an upregulation of anti-nociceptive activity in response to inflammation. Thus, the spinal cholinergic system may be a regulatory site within the anti-nociceptive pathway, and may provide an attractive target for the development of new therapeutic agents. digital.library.unt.edu/ark:/67531/metadc3125/
- Bacterial Challenge in Lumbricus Terrestris: A Terrestrial Invertebrate Immunotoxicity Model.
- A bacterial challenge assay was developed utilizing the earthworm, Lumbricus terrestris, in order to assess potential immunotoxic effects from exposure to specific polychlorinated biphenyl congeners. Earthworms were inoculated with Aeromonous hydrophila, establishing a 10-day LD50. In vitro assays for effects of PCBs on phagocytosis agreed with mammalian studies, demonstrating potent suppression of phagocytosis by the non-coplanar PCB congener 138 and no suppression by the coplanar congener 126. However, when the effects of the two PCB congeners were evaluated for suppression of resistance to a whole animal infection challenge assay, coplanar PCB 126 decreased the ability of L. terrestris to withstand infection while non-coplanar PCB 138 did not. digital.library.unt.edu/ark:/67531/metadc3640/
- Benthic Macroinvertebrates of Temperate, Sub-Antarctic Streams: The Effects of Altitudinal Zoning and Temperature on the Phenology of Aquatic Insects Associated to the Robalo River, Navarino Island (55°S), Chile
- The Cape Horn Biosphere Reserve, within the remote Sub-Antarctic ecoregion is a reservoir of expressions of biological and cultural diversity. Although it is considered one of 24 wilderness areas remaining in the world, it is not free from local and global threats, such as invasive species, and climate change. Field biologists and philosophers associated to the Sub-Antarctic Biocultural Conservation Program and the Omora Ethnobotanical Park, have worked to describe the region's biocultural diversity, linking ecological and philosophical research into education, ecotourism, and conservation, through a methodology called field environmental philosophy (FEP), which integrates ecological sciences and environmental ethics through a 4-step cycle consisting of: 1) interdisciplinary research; 2) composition of metaphors; 3) design of field activities with an ecological and ethical orientation; and 4) implementation of in situ conservation areas. In this context, the purposes of this dissertation were to: 1) provide a comprehensive review of publications regarding the conservation status of aquatic and terrestrial insects at a global scale and with an emphasis in southern South America; 2) study the distribution of benthic macroinvertebrates through the sharp altitudinal gradient of the Róbalo River watershed; 3) describe the life histories of Gigantodax sp (Simuliidae: Diptera) and Meridialaris chiloeense (Leptophlebiidae: Ephemeroptera) in the Róbalo River and to assess the potential effects of climate change on their phenology; and 4) to apply FEP methodology in order to better understand and communicate the intrinsic and instrumental values of freshwater invertebrates in the Cape Horn Biosphere Reserve. digital.library.unt.edu/ark:/67531/metadc103303/
- Beta-adrenergic Blockade Via Atenolol and Its Effects on Blood Pressure, Heart Rate, and Renal Morphology in the Developing Chicken Gallus Gallus Domesticus
- Chicken embryos were chronically exposed to the ?1- blocker atenolol during one of three stages: mesonephros (E7-E9), mesonephros-metanephros (E11-E13), or metanephros (E15-E17). Mesonephros group hearts were larger than all other groups (P < 0.01). Mesonephros and metanephros group kidneys were larger than all remaining groups (P < 0.0001). The mesonephros group nephron number was ~40% lower than control values (P = 0.002). Glomerular areas were 26% and 18% larger than the control group in the mesonephros and metanephros groups, respectively (P < 0.001). These data suggest an E7-E9 critical window of cardiovascular and renal development for atenolol. Acute atenolol exposure in E15 embryos showed an increase in mean arterial pressure with all but the highest dose. All doses significantly decreased heart rate. digital.library.unt.edu/ark:/67531/metadc177248/
- Biochemical Genetics of Certain Species of the Blackbird Family Icteridae
- Starch gel electrophoresis was used to compare 14 proteins encoded by 15 loci for seven species of the family Icteridae. A close genetic relationship among these species was classified into three groups. The Agelaiine group contained Agelaius phoeniceus, Sturnella magna, and S. neglecta. The Quiscaline group contained Euphagus cyanocephalus, Cassidix mexicanus, and Quiscalus quiscula. Molothrus ater, the most divergent, was placed in a separate group. Divergence times for the seven species were compared to the literature. Heterozygosity of the seven populations of the two species of Sturnella were compared to determine factors influencing their divergence. Two factors proposed were heterosis in S. neglecta and possible hybridization between S. neglect and S. magna. digital.library.unt.edu/ark:/67531/metadc663237/
- Biochemical Identification of Molecular Components Required for Cyanide Assimilation in Pseudomonas fluorescens NCIMB 11764
- Utilization of cyanide as a nutritional nitrogen source in P. fluorescens NCIMB 11764 was shown to involve a novel metabolic mechanism involving nonenzymatic neutralization outside of cells prior to further enzymatic oxidation within. Several cyanide degrading enzymes were produced by NCIMB 11764 in response to growth or exposure to cyanide, but only one of these cyanide, oxygenase (CNO), was shown to be physiologically required for assimilation of cyanide as a growth substrate. digital.library.unt.edu/ark:/67531/metadc278624/
- Bioconcentration and Morphological Effects of Triclosan on Three Species of Wetland Plants
- Triclosan (TCS) is an antimicrobial compound found in several types of common household products. After being washed down the drain, TCS will then end up in the local watershed. Although numerous studies have been conducted to evaluate the fate and effects of TCS in aquatic environments, there have been no studies evaluating the role arbuscular mycorrhizal fungi (AM) play in a plants response to TCS exposure. Three species of wetland plants native North Texas were inoculated with AM spores and exposed to 0, 0.4 g/L and 4.0 g/L TCS concentrations. Root morphology of E. prostrata and S. herbacea showed AM and exposure responses. S. herbacea produced the greatest amounts biomass and TCS bioaccumulation, in all but one treatment. It also displayed opposing results to E. prostrata in measures of root length, root surface area, relative root mass, relative shoot mass and shoot:root ratio. TCS root tissue concentrations increased with increased exposures for both E. prostrata and S. herbacea. Even though E. prostrata had the lowest levels in each measure of biomass production, it had the highest amount of root TCS bioaccumulation in the AM inoculated 4.0 g/L treatment. H. laevis was between the other two species in terms of biomass production, and did not demonstrate any exposure or inoculation effects in morphology or TCS accumulation. Overall, no clear patterns were detected, which highlights the fact that further study is required to completely understand the effects compounds like TCS can have on plant community structure, and ultimately ecosystem function. digital.library.unt.edu/ark:/67531/metadc271900/
- Biodiversity of Caddisflies (Trichoptera) of the Interior Highlands of North America
- Caddisflies (Trichoptera) were collected from over 500 different locations throughout the Interior Highlands (Ozark, Ouachita, Arbuckle, and Wichita Mountains) between March 1990 and March 1994. I systematically sampled representative lotic and lentic habitats in 131 natural watersheds that comprise the 17 different physiographic subregions of this area. From my examination of approximately 60,000 specimens, surveys of regional museum collections, and review of literature records, I document 229 species distributed in 16 families and 58 genera. Included in this total are 27 endemic species and 15 new regional records. Descriptions are provided for a species new to science (Cheumatopsyche robisoni), four larvae (Helicopsyche limnella, H. piroa, Marilia species A, Polycentropus crassicornis) and a female (Helicopsyche piroa). Hydropsyche reiseni Denning, previously known only from the Arbuckle Mountains, is reduced in synonymy with H. arinale Ross. Further, I provide illustrated family, generic, and selected species-level keys that reflect this regional biodiversity. digital.library.unt.edu/ark:/67531/metadc278224/
- Biodiversity of Dragonflies and Damselflies (Odonata) of the South-Central Nearctic and Adjacent Neotropical Biotic Provinces
- The south-central United States serves as an important biogeographical link and dispersal corridor between Nearctic and Neotropical elements of western hemisphere odonate faunas. Its species are reasonably well known because of substantial collections, but there has been no concerted effort to document the extent of biodiversity and possible geographic affinities of dragonflies and damselflies of this region. The recent discoveries of Argia leonorae Garrison, Gomphus gonzalezi Dunkle and Erpetogomphus heterodon Garrison from southern and western Texas and northern Mexico suggest that Odonata species remain to be discovered in this area, particularly from far south Texas and northern Mexico. I have documented a total of 12,515 records of Odonata found in 408 counties within the south-central U.S. A total of 73 species of damselflies and 160 species of dragonflies was revealed in the region. The 233 (197 in Texas) Odonata species are distributed among 10 families and 66 genera. Illustrated family, generic, and species-level keys are provided. Since the beginning of this work in the Fall of 1993, one species has been added each to the Louisiana and Oklahoma faunas, and 12 species have been added, previously unreported from Texas, including four new to the U.S. The area of highest Odonata biodiversity overall (161 spp.) is in the Austroriparian biotic province. The greatest degree of faunal similarity between the south-central U.S. and other intra-continental regions was observed for the eastern (64%) United States. Diversity is a function of area, and as expected, the numbers of breeding birds and Odonata, in each contiguous U.S. state are positively correlated (r=0.376, n=33, p=0.031). There is, however, no strong correlation between land area and species diversity within the region, but those natural biotic provinces (Austroriparian, Texan, Balconian) where aquatic systems and topographic heterogeneity are the greatest provide a broader spectrum of potential Odonata habitats and thus support a greater number of Odonata species. digital.library.unt.edu/ark:/67531/metadc501251/
- Biogeographic Relationships of Pocket Gophers (Geomys breviceps and Geomys bursarius) in the Southeastern Portion of Their Ranges
- This research utilized population genetic analyses (protein starch-gel electrophoresis and DNA sequencing of the cytochrome b mtDNA gene), host-parasite specificity (lice coevolution), remote sensing of satellite data, and geographic information systems (GIS) to characterize newly discovered populations of pocket gophers (genus: Geomys) in Arkansas. These populations are isolated and occur in seemingly unsuitable habitat in the Ozark Mountains of Arkansas. Analyses of electrophoretic and ectoparasite data suggested the populations in the Ozark Mountains represented isolates allied to Geomys bursarius, a species not known to occur in Arkansas. Comparison of mitochondrial DNA sequence data of the cytochrome b gene with that of other taxa and morphometric analyses confirmed that these populations are most closely allied to G. bursarius occurring to the north in Missouri. Moreover, these mtDNA sequence analyses indicated a degree of differentiation typical of that between other subspecies of pocket gophers. Therefore, these populations represent a distinct genetic entity in an intermediate stage of speciation and should be designated as a new subspecies, Geomys bursarius ozarkensis. Molecular clock analysis revealed a time of lineage divergence for this new subspecies as approximately 511,000 YBP. Due to the isolated nature and limited distribution of this subspecies, an evaluation of critical habitat needs was initiated. Remote sensing and GIS technologies were used to identify and describe suitable habitat Computerized classification of satellite imagery of suitable vegetation, integrated with ancillary digital information on soil associations, roads, and water systems, revealed that human activity had played a positive role in the establishment and dispersal of pocket gophers in this area. This research represents an initial combination of classical systematic tools with remote sensing and GIS to investigate biogeographic patterns and evolution. This project establishes a framework for using an interdisciplinary approach to studying organisms with limited distributions, determining evolutionary status, and providing recommendations for conservation. digital.library.unt.edu/ark:/67531/metadc500612/
- Biogeography of Montane Mammals on the Colorado Plateau and Adjacent Regions
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This study identifies the biogeographic factors that structure small mammal communities on mountains of the Colorado Plateau and adjacent regions. Forty six isolated ranges were characterized across a 5-state study area encompassing the Colorado Plateau, including the central high plateaus of Utah and the Basin and Range Province (i.e. the Great Basin and mountains of Arizona and New Mexico). Presence/absence data of 25 montane mammal species were used to explore the interactions between historical and ecological processes affecting local and regional diversity patterns. Multivariate analyses, such as non-metric dimensional scaling, were used to explore factors which influence community composition. Results of these analyses revealed the Colorado River as a significant biogeographic barrier that affects montane mammal community structure. MtDNA cytochrome b sequence variation was analyzed among populations of the long-tailed vole, Microtus longicaudus, sampled from five interior ranges of the Colorado Plateau- Abajo, LaSal, Henry, and Chuska Mts., and Boulder Mountain of the Aquarius Plateau-and analyzed using traditional phylogenetic approaches (parsimony and likelihood) as well as nested clade analysis. Results support previous documentation of a major east-west phylogeographic break occurring between populations southeast of the Colorado River (eastern Arizona, Colorado, Wyoming and New Mexico) and all other western populations, which include a central clade, a northwest clade, and an Alaskan island clade. Evidence also supports differentiation of a 'southern Rockies' clade and a distinct 'southwest island' clade. Populations of M. longicaudus north and west of the Colorado River (Boulder and Henry Mts.) share two haplotypes, form a well-supported subclade with populations from the Kaibab plateau, and are closely related to the Northwest clade. Past approaches to studying montane mammal communities utilizing theory based on island biogeography have overemphasized area and isolation as the only forces structuring insular communities. As a result, there has been a lack of recognition of the influences of environmental factors, species turnover, and barriers that create and maintain regional diversity on the Colorado Plateau and adjacent areas. digital.library.unt.edu/ark:/67531/metadc4467/
- Biological Nitrogen Fixation in Two Southwestern Reservoirs
- This investigation has determined the presence of biological nitrogen fixation in two reservoirs in the southwestern United States: Lake Arlington and Lake Ray Hubbard. Subsequent tests have gathered baseline data on the effects of various biological, chemical, and physical parameters on in situ nitrogen fixation in these reservoirs. Of specific importance is the relationship between nitrogen fixation arid occasional blooms of blue-green algae which produce such problems as testes and odors in these water-supply impoundments. digital.library.unt.edu/ark:/67531/metadc278063/
- Biology and Energetics of Tropisternus Lateralis Nimbatus (SAY) (Coleoptera: Hydrophilidae) in a Playa on the Southern High Plains of Texas and Aquatic Coleoptera Diversity from Seven Playas on the Southern High Plains of Texas
- A study of the biology of Tropisternus lateralis, a hydrophilid beetle, was conducted during the flood period of a single playa on the Southern High Plains of Texas from early June 1995 through early September 1995. Mechanism of colonization, tolerance/avoidance to drought, larval density, and secondary production were analyzed. T. lateralis colonized playas from surrounding aquatic habitats and avoided drought through aerial dispersion. Once in the playa, larval density increased over time. Secondary production was 1.31 g/m2/.25 yr. In addition, aquatic Coleoptera diversity was studied in seven playas on the Southern High Plains of Texas. A total of twenty three species were identified from the study region. Nine species not reported in playa literature were identified. digital.library.unt.edu/ark:/67531/metadc277795/
- Blood Pressure Regulation During Simulated Orthostatism Prior to and Following Endurance Exercise Training
- Cardiovascular responses and tolerance to an orthostatic stress were examined in eight men before and after eight months of endurance exercise training. Following training, maximal oxygen consumption and blood volume were increased, and resting heart rate reduced. Orthostatic tolerance was reduced following training in all eight subjects. It was concluded that prolonged endurance training decreased orthostatic tolerance and this decrease in tolerance appeared associated with attenuated baroreflex sensitivity and alterations in autonomic balance secondary to an increased parasympathetic tone noted with training. digital.library.unt.edu/ark:/67531/metadc277914/
- Carbon Flux in Reservoir Sediments
- The central objective of the study was to fractionate sedimenting organic materials according to their source (allochthonous or autochthonous) and ultimately to determine the degree of biodegradability of contributions from either source with particular reference to activities at the mud-water interface. digital.library.unt.edu/ark:/67531/metadc164602/
- A Carcinogenic Agent Elaborated by Liver Cells from Lymphosarcoma-Bearing Mice
- Liver cells from lymphosarcoma-bearing DBA/1J mice were shown, by parabiotic culture with normal liver cells from isologous mice, to elaborate an agent which could pass a 25 mu filter and transform the normal cells to a malignant state. digital.library.unt.edu/ark:/67531/metadc131529/
- Cardio-Respiratory Ontogeny and the Transition to Bimodal Respiration in an Air Breathing Fish: Morphological and Physiological Development in Normoxia and Hypoxia.
- As selection pressures exist for not only adults, but for every life history stage, it is important to understand how environmental factors shape developing animals. Despite the significance placed on aquatic hypoxia as a driving force in the evolution of air breathing, this is the first known study to examine the effects of hypoxia on cardio-respiratory ontogeny of an air breathing fish. Blue gouramis are obligatory air breathing fish that possess a labyrinth-like structure that serves as the air breathing organ. Gouramis were reared for up to 90 d in normoxia or hypoxia, and morphological and physiological development was observed. Hypoxic larvae had increased lamellar and labyrinth organ surface areas. Bradycardia and increased gill ventilation rates were observed when larvae from either rearing group were briefly exposed to hypoxia. Hypoxic larvae also showed a reduced heart rate and gill ventilation rate in the absence of a hypoxic stimulus, possibly indicative of a more comprehensive, long-term respiratory plasticity. The similarity of routine oxygen consumption between rearing groups suggests that metabolic demand did not change for hypoxic larvae, but that they were more efficient at oxygen acquisition. This is further supported by increased resistance time of hypoxic gouramis to extreme hypoxia. The onset of air breathing was between 20 and 25 d post-fertilization, and was not affected by either rearing or exposure environment. It may be that this behavior is associated with the inability of smaller larvae to successfully overcome water surface tension, rather than with the necessity of aerial respiration at this stage. Hypoxia is commonly experienced by most air breathing fishes, and studies of hypoxia-induced developmental effects may provide critical insights into the evolution of air breathing. The studies presented here provide novel data on the plasticity of cardio-respiratory development of an air breathing fish reared in hypoxia, and can serve as a solid foundation for future studies. digital.library.unt.edu/ark:/67531/metadc11056/
- Characteristics of Primary Cilia and Centrosomes in Neuronal and Glial Lineages of the Adult Brain
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Primary cilia are sensory organelles that are important for initiating cell division in the brain, especially through sonic hedgehog (Shh) signaling. Several lines of evidence suggest that the mitogenic effect of Shh requires primary cilia. Proliferation initiated by Shh signaling plays key roles in brain development, in neurogenesis in the adult hippocampus, and in the generation of glial cells in response to cortical injury. In spite of the likely involvement of cilia in these events, little is known about their characteristics. Centrosomes, which are associated with primary cilia, also have multiple influences on the cell cycle, and they are important in assembling microtubules for the maintenance of the cell's cytoskeleton and cilia. The cilia of terminally differentiated neurons have been previously examined with respect to length, incidence, and receptors present. However, almost nothing is known about primary cilia in stem cells, progenitors, or differentiated glial cells. Moreover, it is not known how the properties of cilia and centrosomes may vary with cell cycle or proliferative potential, in brain or other tissues. This dissertation focuses first on neurogenesis in the hippocampal subgranular zone (SGZ). The SGZ is one of the few brain regions in mammals that gives rise to a substantial number of new neurons throughout adulthood. The neuron lineage contains a progression of identifiable precursor cell types with different proliferation rates. This present study found that primary cilia were present in every cell type in the neuronal lineage in SGZ. Cilium length and incidence were positively correlated among these cell types. Ciliary levels of adenylyl cyclase type III (ACIII) levels relative to ADP-ribosylation factor-like protein 13b (Arl13b) was higher in neurons than in precursor cells and glia, and also changed with the cell cycle. G-protein coupled receptors, SstR3, MCHR1, and Gpr161 receptors were only found in neuronal cilia. The levels and distribution of three centrosomal proteins, γ-tubulin, pericentrin and cenexin in neurons was different from the distributions in precursors and glia. The second focus of study is glial responses to injury in the neocortex, which has been widely studied as an injury model. This study found that in the normal adult somatosensory cortex, primary cilia were present in astrocytes and polydendrocytes but not in microglia. Following injury, the incidence of primary cilia decreased in astrocytes. Also, a new cell type expressing GFAP, NG2 and Olig2 was seen 3 days following injury, but was not present in normal mice. The characteristics of primary cilia and centrosome described here suggest that in stem cells and progenitors their characteristics may be well suited for proliferation, whereas in neurons, the cilia and centrosomes are important for other sensory functions. digital.library.unt.edu/ark:/67531/metadc801939/
- Characterization of Arthrobacter Globiformis Aspartate Transcarbamylase Concentrations of Substrates
- This thesis consists of one major section with two subsections. The first subsection investigates the activity of Arthrobacter globiformis aspartate transcarbamylase's specific activity with increasing concentrations of the enzyme's substrate. Dihydroorotase (DHOase) activity was also measured with increasing concentrations of the substrate dihydroorotate. The second subsection collected data in order to classify the enzyme, resulting in a classification into the category of class A ATCases with bifunctional ATCase-DHOase complexes. The thesis provides evidence to broaden understanding of the ATCase and DHOase enzymes for members of the family that Arthrobacter belongs to. digital.library.unt.edu/ark:/67531/metadc271918/
- Characterizing Spontaneous Neurophysiological Activity with Measures of Statistical Serial Dependence: a Summary Statistic and an Extension of the Joint Interval Histogram
- Two measures which indicate statistical serial dependence were evaluated. The n-dimensional Average Stored Information index (ndASI) is a measure of conditional information, which compares the entropies of higher order conditional distributions to estimate average statistical serial dependence. The generalized ranked joint interval histogram (RJIH-o) is a new nonparametric graphical analysis method. It extends the joint interval histogram by depicting longer interval sequences and can be interpereted precisely analagously to the joint interval histogram. The generalized ranked joint interval histogram correctly represents independence in a Poisson process model and statistical serial dependence in a directionally reinforced Markov model. The generalized ranked joint interval histogram correctly depicts the underlying periodic and strange attractors in a standard map model. Both measures can be used to effectively analyze interspike interval sequences from spontaneous neurophsyiological activity effectively. digital.library.unt.edu/ark:/67531/metadc278067/
- Chemotactic Response of Lumbricus terrestris Coelomocytes to Larval and Adult Stages of Rhabditis pellio
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Experiments were performed to assess the suitability of Rhabditis pellio, a nematode found in earthworms, as a challenge organism for use in development of a biomarker assay to determine the potential of chemicals to suppress the immunocompetence of the non-specific immune system. To accomplish this goal, information on the life cycle of R. pellio was determined; including effects of incubation time and temperature on growth rates; along with information on the immune response elicited in the earthworm, Lumbricus terrestris. Immune parameters measured were coelomocyte migration toward and attachment to R. pellio larvae and adults. Preliminary background information showed that R. pellio has potential as a challenge organism for development of a biomarker assay. digital.library.unt.edu/ark:/67531/metadc5507/
- Chronic Hypoxia and Hyperoxia Modifies Morphology and Vegf Expression of the Lungs of the Developing Chicken (Gallus Gallus Domesticus)
- This study determines effects of oxygen levels on morphology and VEGF expression of developing chicken lungs following incubation in normoxia (21% O2), hypoxia (15% O2) or hyperoxia (30% O2), until developmental days 16 or 18. Lung morphology was assessed using light microscopy, while VEGF expression was determined with ELISA. In hypoxia, the proportion of parabronchial tissue and parabronchi including lumina increased from day 16 to 18 (61 to 68% and 74.2 to 82.2%, respectively). Non-parabronchial tissue was higher in hypoxia than in hyperoxia on day 16 (26 to 20%). However, by day 18, there were no differences between groups. VEGF expression was 33% higher in hypoxia than in hyperoxia on day 16 (736 vs. 492 pg/ml). On day 18, VEGF expression was 43% higher in hyperoxia than in normoxia (673 to 381pg/ml), and remained elevated by 40% in hypoxia over normoxia (631 pg/ml). VEGF may be a mechanism by which parabronchial tissue is stimulated from day 16 to 18 following exposure to chronic hypoxia. digital.library.unt.edu/ark:/67531/metadc177224/
- Cigarette Residues Affect Steroidogenesis in Cultured Y-1 Mouse Adrenal Tumor Cells
- This study (1) quantitatively compared steroid production in cultured Y-l mouse adrenal tumor cells exposed to Camel and Carlton-smoke derived residues, and (2) localized the effects in the cell. Basal steroid production was increased by Camel residues but not by Carlton, while ACTH stimulation was interfered with by both residues. Camel basal stimulation was comparable to that of cAMP, and was abolished by Cytochalasin D. The stimulation was also comparable to that of cholera toxin, which activates adenyl cyclase. Results indicate that residue components dissolve in the membrane stimulating adenyl cyclase at a point similar to or before that utilized by cholera toxin for its stimulating effect. digital.library.unt.edu/ark:/67531/metadc663585/
- Classification of Toolmark Surfaces on Zipper Teeth
- This study proposes the classification of the toolmark under the heads of zipper teeth as a subclass characteristic as outlined by the Association of Firearm and Toolmark Examiners (AFTE). Two separate cases in which zipper teeth were found at crime scenes prompted this study. Brass zipper teeth manufactured by YKK were taken from 20 pairs of jeans and studied using a Reichert comparison microscope at 4X power. Photographs were taken and over 750 comparisons made. It was found that the toolmarks on each side on the 20 zippers were unique and independent of all other sides. The observations made in this study indicate that classifying zipper teeth toolmarks as a subclass characteristic is valid. digital.library.unt.edu/ark:/67531/metadc2248/
- Clustering Algorithms for Time Series Gene Expression in Microarray Data
- Clustering techniques are important for gene expression data analysis. However, efficient computational algorithms for clustering time-series data are still lacking. This work documents two improvements on an existing profile-based greedy algorithm for short time-series data; the first one is implementation of a scaling method on the pre-processing of the raw data to handle some extreme cases; the second improvement is modifying the strategy to generate better clusters. Simulation data and real microarray data were used to evaluate these improvements; this approach could efficiently generate more accurate clusters. A new feature-based algorithm was also developed in which steady state value; overshoot, rise time, settling time and peak time are generated by the 2nd order control system for the clustering purpose. This feature-based approach is much faster and more accurate than the existing profile-based algorithm for long time-series data. digital.library.unt.edu/ark:/67531/metadc177269/
- Coelomic Fluid Protein Profile in Earthworms Following Bacterial Challenge.
- Proteomic techniques were used to evaluate the protein profile of the earthworm, (Lumbricus terrestris), following a bacterial challenge. One control group received no injection; a second control group received injections of phosphate buffer solution (PBS). The experimental group received injections of PBS containing (Aeromonas hydrophila). After incubation for 12 hours at 20°C, coelomic fluid was collected from each group for analysis by 2-D electrophoresis. There were significant differences in spot appearance and density between control and experimental groups. Sixteen spots showed a two-fold increase in density and 63 showed at least a two-fold decrease in density between samples from control and bacteria-challenged earthworms, respectively, suggesting up- and down-modulation of proteins potentially involved in the earthworm's response to bacterial challenge. digital.library.unt.edu/ark:/67531/metadc5476/
- College Freshman Biology Two Semester Course: Integrating Deep Processing Teaching Techniques
- Development of a college level freshman biology course was undertaken in response to government reports that American students have fallen behind students of other countries in the area of the sciences. Teaching strategies were investigated to accomplish two objectives, to define essential academic material to include in the course and to investigate teaching techniques that would increase deep processing of the information. An active process that consisted of applying the cognitive information to solving problems or developing answers to questions was defined as critical thinking. Critical thinking was incorporated into the course by the use of case studies. digital.library.unt.edu/ark:/67531/metadc3158/
- Comparative biochemistry and genetic analysis of nucleoside hydrolase in Escherichia coli, Pseudomonas aeruginosa, and Pseudomonas fluorescens.
- The pyrimidine salvage enzyme, nucleoside hydrolase, is catalyzes the irreversible hydrolysis of nucleosides into the free nucleic acid base and D-ribose. Nucleoside hydrolases have varying degrees of specificity towards purine and pyrimidine nucleosides. In E. coli, three genes were found that encode homologues of several known nucleoside hydrolases in protozoa. All three genes (designated yaaF, yeiK, and ybeK) were amplified by PCR and cloned. Two of the gene products (yeiK and ybeK) encode pyrimidine-specific nucleoside hydrolases, while the third (yaaF) encodes a nonspecific nucleoside hydrolase. All three were expressed at low levels and had different modes of regulation. As a comparative analysis, the homologous genes of Pseudomonas aeruginosa and P. fluorescens (designated nuh) were cloned. Both were determined to encode nonspecific nucleoside hydrolases. The nucleoside hydrolases of the pseudomonads exhibited markedly different modes of regulation. Both have unique promoter structures and genetic organization. Furthermore, both pseudomonad nucleoside hydrolase were found to contain an N-terminal extension of 30-35 amino acids that is shown to act as a periplasmic-signaling sequence. These are the first two nucleoside hydrolases, to date,that have been conclusively demonstrated to be exported to the periplasmic space. The physiological relevance of this is explained. digital.library.unt.edu/ark:/67531/metadc3290/
- Comparative Chemistry of Thermally Stressed North Lake and Its Water Source, Elm Fork Trinity River
- To better understand abiotic dynamics in Southern reservoirs receiving heated effluents, water was analyzed before and after impoundment in 330 ha North Lake. Macronutrients, metals, and chlorinated hydrocarbons were measured. Concentrations of nutrients and metals in sediments were quantified in this 2 yr study. River water prior to impoundment contained 16 times more total phosphorus, and supported 23 times more Selenastrum capricornutum cells in an algal assay than reservoir water. The reservoir has essentially no drainage and since evaporation is high, the concentrations of many dissolved solids have increased since the reservoir was filled in 1958. North Lake is now phosphorus limited. Apparently altered chemical equilibria have caused precipitation or adsorption of phosphorus with calcium and iron. digital.library.unt.edu/ark:/67531/metadc663687/
- Comparative Morphology of Sensilla Styloconica on the Proboscis of North American Nymphalidae and Other Selected Taxa: Systematic and Ecological Considerations.
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Sensilla styloconica on the proboscis of 107 species of North American and tropical butterflies were comparatively studied using the scanning electron microscope. Focus was on 76 species of North American Nymphalidae representing 45 genera and 11 subfamilies. Nomenclature for generalized and specific types of nymphalid sensilla is proposed. Written descriptions and micrographs are presented for each species studied. Morphological features were generally consistent for all or most species within genera and sometimes within subfamilies, with specified exceptions. Statistical analysis revealed significant differences for six of eight variables tested between two distinct feeding guilds of North American Nymphalidae. Average number, density, extent of proboscis coverage with sensilla, their total length, and shoulder spine length were all significantly greater in the non-nectar feeding guild than in nectar feeders, and may indicate adaptation for greater efficiency in feeding on flat surfaces. The greater frequency of apical shoulder spines in non-nectar feeders may represent adaptation for protection of sensory pegs from mechanical abrasion during feeding, or for anchoring the flexible proboscis tip to the surface. Correlation analysis revealed 9 out of 28 positive correlations in nectar feeders and 5 out of 28 in non-nectar feeders. Results of preliminary cladistic analysis were not considered to be meaningfully robust due to few available characters. The stylar characters identified in this study should be more useful in future analyses when included with characters from other lines of evidence. The presence of sensilla styloconica in all subfamilies of Nymphalidae, except Danainae, largely supports Ehrlich's (1958) higher classification concept for the family. The presence of less conspicuous sensilla in the Danainae, and other characteristics are presented as further evidence that they should be reconsidered for full family status. Sensilla styloconica in nymphalid butterflies appear to function as extensions that provide greater sensory reach during feeding. The role of these sensilla in liquid uptake, pollen feeding, and host plant selection is discussed. digital.library.unt.edu/ark:/67531/metadc3002/
- A Comparative Study of Passive Transfer Mechanisms of Tuberculin and Chemical Contact Delayed Hypersensitivities in the Guiea Pig
- This study is concerned with a critical comparison of the passive transfer mechanisms of tuberculin and chemical contact hypersensitivities in the guinea pig by use of a four phase experimental approach. digital.library.unt.edu/ark:/67531/metadc164409/
- Comparison of Aspartate Transcarbamoylase and Pyrimidine Salvage in Sporosarcina urea, Sprolactobacillus inulinus, Lactobacillus fermentum, and Micrococcus luteus
- The enzyme that catalyzes the committed step in pyrimidine biosynthesis, aspartate transcarbamoylase, has been compared in selected endospore-forming organisms and in morphologically similar control organisms. The ATCases and pyrimidine salvage from Sporosarcina ureae, Sporolactobacillus inulinus, Lactobacillus fermentum, and Micrococcus luteus were compared to those of Bacillus subtilis. While the ATCases from Sporosarcina ureae, Sporolactobacillus inulinus, and L. fermentum were found to exhibit characteristics to that of Bacillus with respect to molecular weight and kinetics, M. luteus ATCase was larger at approximately 480 kDa. Furthermore, pyrimidine salvage in Sporosarcina ureae and M. luteus was identical to those of B. subtilis, while pyrimidine salvage of Sporolactobacillus inulinus and L. fermentum resembled that of the pseudomonads. digital.library.unt.edu/ark:/67531/metadc278938/
- Comparison of Heteranthera Dubia (Jacq.) MacM.-associated Macroinvertebrates Between Georgraphical Regions in the United States
- Macroinvertebrates associated with the aquatic plant, water stargrass (Heteranthera dubia), were sampled from 12 waterbodies in four regions of the United States from June to August 2005. Taxa richness, evenness, and diversity were lowest in the Lower Midwest (LMW) region, and higher in Northern sites, especially the Upper Midwest (UMW), and Northeast (NE). While relative abundance varied from site to site and region to region, utilization of the plant by functional groups remained fairly constant. Collector-gatherers consistently comprised the largest portion of invertebrates sampled. The shredder/ herbivore functional group comprised an average of 17 % of total groups. Through an exhaustive literature review, it was found that shredder/ herbivores of water stargrass have not been reported in the literature. Because of this, the herbivore group was analyzed separately and consisted of 2,383 specimens representing 23 species. The most common groups were Rhopalosiphum sp., Nectopsyche spp. and chironomids. No differences were found in herbivore diversity or evenness between sampling regions, but species richness was significantly different. digital.library.unt.edu/ark:/67531/metadc28427/
- Comparison of Remediation Methods in Different Hydrogeologic Settings Using Bioplume II
- A contaminant fate and transport computer model, Bioplume II, which allows simulation of bioremediation in ground water systems, was used to compare the effects of 11 remediation scenarios on a benzene plume. The plume was created in three different hydrogeologic settings from the simulation of an underground gasoline storage tank leak. digital.library.unt.edu/ark:/67531/metadc278039/
- Concentration-dependent Effects of D-Methylphenidate on Frontal Cortex and Spinal Cord Networks in vitro
- Spontaneously active frontal cortex and spinal cord networks grown on microelectrode arrays were used to study effects of D-methylphenidate. These central nervous system tissues have relatively low concentrations of dopaminergic and noradrenergic neurons compared to the richly populated loci, yet exhibit similar neurophysiological responses to methylphenidate. The spontaneous spike activity of both tissues was inhibited in a concentration-dependent manner by serial additions of 1-500 µM methylphenidate. Methylphenidate is non-toxic as spike inhibition was recovered following washes. The average concentrations for 50% spike rate inhibition (IC50 ± SD) were 118 ± 52 (n= 6) and 57 ± 43 (n = 11) for frontal cortex and spinal cord networks, respectively. A 3 hour exposure of a network to 1 mM methylphenidate was nontoxic. The effective concentrations described in this study are within the therapeutic dosage range. Therefore, the platform may be used for further investigations of drug mechanisms. digital.library.unt.edu/ark:/67531/metadc4689/


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Medieval Magic
Set in medieval times, a group of seven heroes must come together to stop a barbarian horde.My Thoughts:
I don't know why I didn't look into this series last month when it debuted. This series is awesome. With medieval times, magic and dark sorcery how could it not be awesome? While I haven't heard of some of these characters, Paul Cornell does a great job introducing newer readers to them in such a phenomenal and accessible way. Each character is intriguing and has their own voice and personality. They appear to be well defined characters I can't wait to get to know better.
Etrigan seems to be the main character as he's centered on the cover. I thought it was cool how Cornell merged Jason and Etrigan together. From the first time Jason released the demon I knew I was going to love this character. Sir Ystin (Shining Knight) is another character that caught my eye. She was first introduced in Grant Morrison's Seven Soldiers (which I still have to read). The other characters are equally interesting. I'm really digging the premise and can tell Cornell will shine in this series which seems much more interesting than his Stormwatch. How can it not be great when the issue ends with an angry group of dragons smashing through the inn?
Diogenes Neves handles the art which can be summed up in one word; breathtaking. This man is an extraordinary artist who applies such an ample amount of detail within his panels. He does a great job capturing the time period as well. The colors by Mareclo Maiolo are gorgeous. Tony Daniel's cover is outstanding. I can't wait to see more from the art team.
This series is like a diamond in the rough. It's possibly my new favorite series out of the new 52. I highly recommend giving this series a chance. You won't be disappointed especially if you're into medieval times.Rating: 5/5

subset: commoncrawl


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4960 Peachtree Industrial Blvd Ste 240
Berkeley Lake, GA, 30071 United States
(678) 620-3186 †
Galectin Therapeutics has a knack for inhibiting galectin proteins. The drug developer is targeting galectin proteins as they play a key role in the development of a variety of diseases. It is developing such inhibitors to treat liver fibrosis, which is currently untreatable. Its GM-CT-01 drug candidate is being investigated for use targeting certain melanomas and in combination with another drug to improve its effectiveness in treating colorectal cancer. Galectin Therapeutics is focused on diseases with serious, life-threatening consequences and on diseases for which there is little to no current treatment available.
† Some telephone numbers on the Hoover's site may be on a country's do not call or do not contact list including, but not limited to, the United Kingdom's CTPS or TPS registers. It is a legal requirement that companies do not make sales or marketing calls to registered numbers. These are central opt out registers whereby corporate subscribers and individuals can register their preference not to receive unsolicited sales and marketing telephone calls. By using the information provided on the Hoover's sites, as the direct marketer you represent and warrant that you will use such information in compliance with all applicable local, state, national or international laws and regulations, including any local do not call registers or marketing regulations, and agree to defend, indemnify and hold harmless Dun & Bradstreet and each of its affiliates in the event your use violates such laws and regulations.


text:
you gonna eat that?
Popularity: The Baskie Quick GuideFebruary 16th 2005 4:46 pm
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This month's missive is dedicated to that je ne sais quack -- that certain something that lets you get away with anything, and never really suffer any consequences...
tips on a happy turkeydayNovember 24th 2004 1:11 pm
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so this is the thing...it's like totally that time when humans pig out, and blatantly ignore the anguish all that cooking smell does to us (i mean, why cook it, when it's better raw? -- must be to torment us with smells!).
hangin out with the momsNovember 23rd 2004 11:22 am
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sunday 11/21/04: just another lovey day in the neighborhood (and remember, the whole world is my neighborhood...). we went for a romp in pacifica, and i made it to montara before my mom's voice got too agitated. dude, it's a bummer when she gets upset like that. plus is was kinda time for dinner.
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text:
Current Round
Min Deposit: 1.00 Max: 20 Skins
cs go skin store
If you think about it, it makes a lot of sense. The screenshot above is a random selection of items from G2A's store, numbering up to hundreds of dollars. These are items that players will just have sitting around, some of them going unused, some of them dearly held collectors items, some of them in the hands of those totally unaware of what they're worth. Those players are already customers of these stores and will be looking to buy and sell at all times. If Kinguin and G2A can create a marketplace better than the other options out there, provide protections and services that others cannot due to their higher budget, that's money on the table.The global arms trade is one of the most lucrative markets in the world, but two young gamers have discovered the virtual weapons market isn't too shabby either.The first thing you will want to do is sign up for a free account over at Twitch.tv. You aren't required to have an account to watch or comment on streams, although you do get more features by signing up for one. In addition to creating your own videos, signing up for an account will allow you to message and interact with other users, follow streams, and more. Once you have created your account, I recommend going to the Twitch settings, clicking on the Channel and Videos tab, and checking the "Automatically archive my broadcasts" box. This won't save old streams forever, however they will be stored on your channel for the next 14 days. You will also have the ability to export older broadcasts to YouTube and create video highlights that will be saved on your Twitch channel forever.That's another important point about DirectX 12: the benefits it confers, it confers on developers. And the nature of game development, as Gaslamp Games' programmer Nicholas Vining pointed out, is "as soon as you get a game running at 60 FPS, your designers and artists will immediately knock it back down to 30 FPS."Valve is known for taking its time on projects. The "Steam Machine" was announced a couple of years ago as a joint hardware-software PC solution for living room set-top gaming with, perhaps more interestingly, promised inclusion of a haptic-enabled Steam controller. This controller would be the cause of many delays in the pipeline as Valve faced design unique design and usability challenges.An incident with performance-enhancing drugs was inevitable for esports, which are growing more than ever alongside the popularity of competitive games and livestreaming. ESL's stopgap measure of implementing random tests for ESL Cologne in August is welcome, but how will drug testing be handled going forward? How will a league like ESL react during a tournament weekend when one of its players tests positive for a banned substance?
Operation Wildfire l?uft übrigens bis zum 17. Juni 2016. Bis zu diesem Tag sind die sieben Community Maps auf den offiziellen Servern von Valve spielbar sowie die Mission erfüllbar.In einem eigenen Guide zum Thema Wallbanging hat Valve das Remake von Nuke im Juli 2014 erstmals angeteasert. Das Remake hat sich laut Aussage von Jess Cliffe seither stark ver?ndert. Die neue Version von Nuke ist wahrscheinlich die optisch beste Map in CS:GO. Das alte, graue Aussehen ist einem modernen Look gewichen, bei dem Valve nicht mit Details gegeizt hat.The benefits, though, are obvious. Players are encouraged to be as open as they like about anything within the origanisation, because it is theirs. They're less likely to have a quick Reddit or Twitter pop-off from being disgruntled too. The main question being lobbied is what's the deal with roster changes inevitably happen, and there's due to be an AMA where these sorts of details are answered tonight. Companies part- or wholly-owned by their employees are hardly a new idea though, so there will be defined structures for them to follow.I don't expect you to watch that, so here's a handy summation of what was discussed during the conversation: Seltene Items in CS:GO anzeigenHere is a simple step-by-step guide in wiping out the extension from Google Chrome:
In my personal opinion Cluj-Napoca was a fantastic showcase of competitive CS:GO. It was the Major for upsets as top teams were sent packing early on in the competition. The level of CS:GO play seemed to be at all time high, but what went wrong? Last night I saw an interesting video which included info-graphicsFor the first time in years, Counter-Strike: Global Offensive is getting its models and animation systems overhauled.However, while the Xbox One CPU is marginally faster, the PS4 has a speedier GPU and a more advanced form of onboard memory – or RAM – (GDDR5 as opposed to DDR3). At the moment it seems that the Xbox One may allocate slightly more RAM to games and less to its operating system (though the PS4 allocation is flexible) and its memory performance is boosted by additional ESRAM, which Microsoft says gives a more efficient memory allocation.The finishers in Mortal Kombat X are more gruesome than ever. On some level that should be an obvious improvement, since this is the first game in the series to show up on this generation of hardware. But the developers have really gone the extra mile to make sure most of the fatalities go "too far" in new ways, from bodies getting ripped in half to faces being sliced off to reveal a tongue that sits there and twitches until you move forward to the next screen. The game also has brutalities, which are new--they aren't just the weird, sped-up dial-a-combo explosions that they were when they showed up in Mortal Kombat Trilogy.Brutalities are a fun way to style on your opponent because you do them during a fight's conclusion instead of waiting for it to shout "finish him" at you. Some of these are easy. For example, if you continue holding forward while ending your winning round with Erron Black's throw, he'll pull out his six-gun and shoot his victim's leg clean off. If you mash some buttons while winning a fight with Takeda's throw he'll rip off his opponent's arm at the end of it. Most of the brutalities are expanded, murderous takes on a character's special moves, so each character has around five brutalities. Many of them have more elaborate requirements, like not getting hit for seven seconds during a match or doing 30% of your damage with one move. Those requirements remind me of the way you'd need to hold down a button for 30 seconds to get Shang Tsung to morph into Kintaro for a fatality--you need to set up how, exactly, you're going to win before you've actually won. In some cases, that makes them risky and, when you pull them off, kind of impressive. They're also a lot faster than the standard fatalities. Faction Kills are also faster than the fatalities, and these tie into an overarching metagame where you pick one of five different factions and all your fights, online or off, deliver points into an overall pool. Once a week, the points are tallied and the winner gets access to a specific faction kill. It's a nice distraction that helps give all of your efforts a little more meaning, even if you're just playing through the story or grinding it out against the AI on one of the many different towers.The man from Canada goes by the online name Johnb32xq. In the eyes of some he is what's wrong with online multiplayer gaming. Some might brand him a scourge—maybe even a bad person—but that's the common reaction to situations like these, when good people cross lines that other good people don't."Instead of being able to change your CS:GO Double theme, your items from your inventory are getting stolen," says Blaze in his blog post. "[I]nstead of trading with X or Y person you trust, the items then go to the scammer rather than whoever you're trading with."As the extensions' names imply, the scam is aimed at users of "Counter Strike Global Offensive" (CS:GO). The scammer with a public display name Delta advertised these extensions as auto-gambling bots or as helpers for carrying out Steam trades.
"We couldn't be happier to be back in CS:GO," CompLexity Gaming founder and CEO Jason Lake said. "Our love for the franchise is what lead to compLexity's formation and we're humbled and honored to be a part of the game once again. We're also very excited to announce our new roster and eager to tackle the challenges ahead. Everyone involved understands that the division will be a work in progress, but developing talent and building champions are areas in which we excel." The original compLexity CS:GO team was acquired by Cloud9 on August 1 of last year. According to a Daily Dot report, the players were "disgruntled" by management policies, which among other things excluded them from any revenues earned through in-game sales of compLexity CS:GO stickers at that year's ESL One Katowice event. But bygones appear to be bygones."ESL One Cologne is going to be the largest Counter-Strike: Global Offensive event in the world," he said. "Taking place in the biggest indoor arena in Germany, we're sure it'll be a massive hit for fans from all over."
"All other in-game items that involve at least one of the contributors in their revenue share have been discontinued.Sadece CS:GO skin'lerine y?nelik olan bu platfromda, en büt?e dostu skin'lerden, her CS:GO oyuncusunun hayallerini süsleyen Dragon Lore ve Bayonet Fade gibi koleksiyon e?yalar?na kadar uzanan geni? bir ürün yelpazesi tüm CS:GO oyuncular?n? bekliyor.ücretsiz kay?t olarak kullanabilece?iniz skins.kinguin.net adresinden ihtiyac?n?z olmad???n? dü?ündü?ünüz skin'lerinizi satabilir ve hayallerinizi süsleyen muhte?em e?yalar? do?rudan, kutu ile u?ra?madan sat?n alabilirsiniz. Sat?n al?m sonras?nda ya?ayabilece?iniz her türlü durumla ilgili olarak ise 7/24 destek hatt?ndan faydalanabilirsiniz.For me, I've been playing FPSess for 12 years now, and I want it to try something different. I wanted to do something different with TI. What my objective was with TI was to explore some different game mechanics and modes. I think we succeeded with some of the elements of TI, but some of the other stuff we tried didn't really pan out so well at all.While Valve is familiar with issuing adjustments to the game, when CS:GO began to expand from smaller league tournaments to international majors, a void was formed that organizations like FIFA and the NBA fill in, i.e. the regulation of professional play. This institutional evolution was awkward, to say the least—forcing a few "uncompetitive maps" in majors here and there, as well as the occasional pro player cheating ban without a word—and this would become even more obvious with the match-fixing incident.The Daily Dot report that Wolski left the team due to a dispute involving months of pay owed by his former team Supa Hot Crew—a team linked to MYM through the company AK3 GmbH. A number of current and former MYM staff are employees of AK3, and Wolski said he was worried about similar issues with his new team.Artur Minacov, 21 and John Brechisci, 28, founded a site called OPSkins in January, and say they've since made a fortune buying and selling virtual video game gun skins online.
"As the scene grows, it's an unfortunate reality that some individuals will seek opportunities to take advantage of their fans," the statement says. "We will continue to take whatever action we think is necessary to protect the entertainment value created by professional Counter-Strike, including, on occasion, terminating our relationship with individuals who have demonstrated a willingness to exploit their fans' faith in the integrity of the sport."Artur Minacov, 21 and John Brechisci, 28, founded a site called OPSkins in January, and say they've since made a fortune buying and selling virtual video game gun skins online.Skin Betting: Will Esports lose its shirt?It'd be fair to say that the regulated betting industry sees Esports betting as 2015's hot topic. Perhaps in one year's time it will have exploded or perhaps it will be consigned as a flash in the pan topic for gambling companies just as social gaming has been. In reality, Esports betting hasn't just emerged over the past few months but has seen huge betting volumes wagered through unregulated markets since the opening of DotaLounge and more significantly, CSGOLounge, in August 2013, 12 months after the game's release. It is not a coincidence that viewership of professional CounterStrike matches has grown from around 30,000 viewers to 500,000 viewers since the introductions of skins to the game and the various skin betting websites opened. No such websites undertake any age verification or form of KYC.the market defines the value of the items. This generally reflects how difficult it is to get an item. Valve's own terms and conditions also reflect this expectation. Skins are generated when a user purchases both a case and a key from Valve, the game's publisher. They run a quasi-slot machine mechanic, where their case and key, worth around £2, becomes a random item. Generally speaking, the rarer the item, the higher the value.ʏһҳúskins for csgo
Ђһҳúcs go buy items


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Topic: advocacy
Powerful Anti-Violence Film Wants You to Unload Gun Companies From Your 401(k)
'They will respond to economic pain'
By David Gianatasio on May. 6, 2014 - 8:57 AM EDT
Unload Your 401(k), a powerful three-minute film from Grey New York, takes aim at a new target in the debate over guns in America: the pocketbooks of firearms companies. The video…
Would You Recognize a Loved One Dressed Like the Homeless? These People Didn't
Hidden-camera stunt aims to 'make them visible'
By David Griner on Apr. 23, 2014 - 4:45 PM EDT
Most city dwellers tend to avoid eye contact with the homeless, a fact that made one advocacy group wonder: Would you recognize your own relatives if they were living on…
How Do You Break Through Apathy? One Agency Tries for Rage
Stunt tricks Londoners into rallying for the poor
By Rebecca Cullers on Apr. 10, 2014 - 11:26 AM EDT
Pleas to help the poor are usually ignored. So what if you turned things around and started advocating against the poor? Would anyone come to their defense? Publicis London put that…
New Attention-Grabbing Mannequins Are Modeled After Disabled Public Figures
'Because who is perfect?' advocacy group asks
By Rebecca Cullers on Dec. 4, 2013 - 9:56 AM EST
It was Junot Diaz who said, "If you want to make a human being into a monster, deny them, at the cultural level, any reflection of themselves." So Pro Infirmis,…
Chinese Ad Campaign Urges People to Stop Eating Dogs and Cats
It's apparently a serious problem
By David Griner on Nov. 14, 2013 - 11:10 AM EST
"Say no to dog or cat meat." That's the message in 279 new ads being plastered across Chinese train stations, bus stations and elevators by a pet advocacy group called…
'Skip' Button Shows How Easily Job Interviewers Can Ignore Ex-Cons
Would you hear him out?
By David Gianatasio on Oct. 24, 2013 - 11:14 AM EDT
If job interviews had a skip button, would anyone be willing to hear out an ex-con? That's the question Leo Burnett and a U.K. nonprofit try to make you answer…
Branding the Next Pro-Choice Generation
Campaign marks 40th anniversary of Roe v. Wade
By Katy Bachman on Jan. 23, 2013 - 5:22 PM EST
It would have been more predictable if Naral Pro-Choice America chose to commemorate the 40th anniversary of Roe v. Wade—the historical Supreme Court decision that affirmed a woman's right to…


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1 Pet Care Job Found
Dog sitter
Posted by Sherry L. on 1/5/2017
- Start date:
- 17 miles away (W Terre Haute, IN)
- $10/visit
- 1 pet
I am looking for someone to either keep my moms dog overnight at their house or visit her dog while she is gone. Mostly weekends. Sometimes 1 night sometimes two in a row. The dog is a small sweet older chihuahua mix. Very loving and happy dog. Needs brief walk outside and food water... More


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Giga Solar Materials to start production at factory in eastern China at end of March
Green energy | 11min ago
China market: Foxconn to produce bicycles for startup Mobike
IT + CE | 23min ago
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Motech Industries to issue 50 million new shares
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China 2016 economic growth hits 25-year low
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Foxconn Electronics chairman clarifies reported Sharp's ending supply of TV panels for Samsung Electronics
Before Going to Press | Jan 23, 21:30
Sharp president reveals focus for fiscal year 2017 operation, says Nikkei
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Casetek obtains chassis orders from top-5 China smartphone vendors
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24-Jan-2017 markets closed
|TSE electronic
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What are LED firms doing in 2017?
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In addition to pure display makers such as Amtran, Chi Mei, HANNspree, Taiwan-based home appliance makers such as Tatung, Proton, Kolin, several major IT makers...
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Taiwan-based display makers come from a variety of areas in the IT industry, from Compal, which is best known for producing notebooks, to Premier, which also produces...
- Wireless broadband developments in Southeast Asia markets
As of 2013, the 10 ASEAN nations had a total of over 700 million mobile subscriptions, with the CAGR from 2003-2013 reaching 24%. This Digitimes Research Special Report analyzes the various mobile broadband markets in ASEAN and looks at the respective trends in 4G LTE development for those markets.
- 2015 global tablet demand forecast
This Digitimes Research Special Report provides a 2015 forecast for the global tablet market and analyzes the strategies of key market players such as Google, Apple, Intel, and Microsoft.
- 2015 China smartphone panel trend forecast
This Digitimes Research Special Report analyzes the strategies of key China-based major panel makers BOE, Tianma and IVO for attacking the different market segments through technology and pricing, and their relationship to local vendors Huawei, Lenovo, ZTE, Xiaomi and Coolpad.


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Until 2 July 2017 at the Rockox House.
During the latter part of the sixteenth century and the early part of the seventeenth, the Scheldt city of Antwerp enjoyed an especially favourable artistic and economic climate that made it the prime production and trading centre for luxury articles. It was a time when many patricians and merchants built up rich collections of contemporary and ancient art, though the majority of those collections have – alas – come to be dispersed in the course of time.
A luxurious art cabinet
From 2 February 2013, however, visitors to the Rockox House in Antwerp will be able to see how an Antwerp art collection must have appeared in the Golden Century. More particularly, the residence of burgomaster and patron Nicolaas Rockox (1560–1640) is being transformed into a luxurious art cabinet with top items from the Royal Museum of Fine Arts Antwerp(closed for renovation) and the most important works from the Rockox House itself. On display will be a range of fine paintings by such masters as Rogier Van der Weyden, Hans Memling, Jan Van Eyck, Peter Paul Rubens and Antoon Van Dyck.
A mini-documentary shows you how this unique exhibition came to be. Museum curators Hildegard Van de Velde (Rockox House Museum) and Nico Van Hout (KMSKA) tell you more about Nicolaas Rockox, collecting art in the low countries, the title of the exhibition and the painting A collection by Frans Francken II.
This is Nicolaas Rockox
This video is an introduction to the exhibition for children: what is the Rockox House exactly and what will I see over there? The video opens up like a book on the life of Nicolaas Rockox.
This is Nicolaas Rockox is part of the educational programme for primary schools. Teachers can show the video to their pupils in class. That way, they will recognize lots of things in the Rockox House Museum and feel more at ease during their visit.
Create your very own art cabinet
For this exhibition, the Royal Museum of Fine Arts Antwerp has developed an online application for smartphone, tablet and pc. By surfing to www.hetguldencabinet.be you will discover all there is to know about the artworks in featured in The Golden Cabinet. Stroll through the rooms of Museum Rockoxhouse with your smartphone or tablet, and gain quick and easy access to information about all the items on show. Zoom in for close-up views of paintings and sculptures. Select your favourite pieces before, during or after your visit and compile your own private collection, just like Nicolaas Rockox did throughout his life. If you launch the webapp from a pc or laptop at home, you can arrange your favourite paintings and objects in your personal art cabinet using a picture of your own living room, dining room or even bathroom. There are attractive monthly prizes to be won for the best cabinets.
Visitors' guide
Download the visitors' guide (PDF, 20 MB)