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Brazil Hopes To Ban Sexist "HOPE" Campaign
But how will women control their men if not through strategic lingerie placement? Featuring Brazilian supermodel Gisele, the ads say women should give their husbands hope by couching "bad news" with sexy outfits. Because women are objects, right guys?
This post was created by a user and has not been vetted or endorsed by BuzzFeed's editorial staff. BuzzFeed Community is a place where anyone can post awesome lists and creations. Learn more or post your buzz!
- White House Press Secretary Sean Spicer repeated claims that the inauguration was the most watched ever. But the numbers (still) don't make sense.
- Britain's Supreme Court has ruled that Prime Minister Theresa May needs to consult parliament before triggering Brexit.
- "The Great Liberation Of France" is a private chat room that Trump Supporters are using to manipulate French voters 💬👀
- 2017 Oscar nominations for Best Picture include: "La La Land," "Moonlight," "Arrival," and "Hidden Figures" 🎥 🎬

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Lucas North / Richard Armitage. // *scroll scroll scroll* * pinch pinch* *scroll scroll scroll...*
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Oh, you lovely, lovely man. Richard Armitage, photo: Joe McGorty, 2010 (promo Spooks for Sunday Times).


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Photo of slain Taliban chief posted on jihadi websites
Islamabad: A photograph purportedly released on Monday by the Pakistani Taliban shows its slain chief Hakimullah Mehsud after he was killed in a US drone strike in North Waziristan region.
The photo, whose authenticity could not be independently confirmed, shows Mehsud lying on a white sheet with bloodstains on his face. It was posted on several jihadi websites and a Facebook page.
Mehsud was killed with other militants when a CIA-operated spy plane targeted a compound in the lawless North Waziristan agency on Friday. His death threw into disarray the Pakistan government`s plans to hold talks with the Taliban.
Known as a ruthless commander, Mehsud first came to prominence after orchestrating audacious raids against the Pakistan Army.
He started as a bodyguard to Baitullah Mehsud, another Tehrik-e-Taliban Pakistan chief who was killed in a drone strike in 2009, and later became his close aide.
Mehsud also served as a spokesman for Baitullah before succeeding him.
It was in 2007, after the capture of 300 Pakistani soldiers, that Mehsud`s name and prestige among the Taliban rose dramatically. He was made the Taliban commander in Khyber, Orakzai and Kurram tribal regions and was regarded as the mastermind behind attacks on NATO supply trucks.
In 2010, Mehsud appeared with a Jordanian militant in a farewell video in which the latter claimed responsibility for a suicide attack in Afghanistan that killed seven CIA agents and contractors.
He was blamed for numerous terror attacks in Pakistan that killed thousands of people and security personnel. Security analysts say he brought the largely Pakistan-focusing Taliban closer to al Qaeda.
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2016 #WSOP Event 59
Yue Du, a 48-year-old venture investor from China and an ardent cash game recreational player, won his first bracelet and a whopping $800,586 in first prize money for taking down the $5,000 buy-in No-Limit Holdem event in a spectacular performance that included busting out all eight of his final table rivals.
The event attracted a stellar field of 863 entrants, generating a prize pool of $4,056,100 and enabling 130 finishers to cash.
It was also notable for the unusual sight of five-bracelet professional ace Jason Mercier kneeling at the table to propose marriage to Natasha Barbour, who had just claimed her $348,374 third-placing check in the event. Not that Mercier needs the cash - hes been doing very well in WSOP this year, adding to career earnings of several millions. She accepted btw.
Back to Dus big win....the Chinese amateur swept all before him in the final days of the event to start the last day with more than half the chips in play.
He promptly put his massive advantage to work when the final table formed as he methodically eliminated every opponent - including three-bracelet German ace Dominik Nitsche - to end in the heads up against Canadian Michael Gentili with a major chip lead.
That ensured a swift departure for the Canadian with his biggest career reward yet - a second placing check for $494,797.
Other final table pay days included:
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More computers seized in police online gambling raids were trashed Monday when the Serdang police destroyed 1,312 machines to keep them out of circulation.
2017 Aussie Millions Event 1 -
The Melbourne-based player bested a field of 1,565 entries - 245 up on last year's equivalent - to end up in the heads up with New Zealnad's Qiang Fu, but not before both he and Qiang had narrowly escaped at the four-handed stage when they were the low stacks.
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The Legal Affairs Committee of the Swiss National Council has nixed new regulations which would have blocked access to online gambling sites in Switzerland, according to local media reports Tuesday.
Growth Ahead For Online Gambling -
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text: LottaRewards.com News http://www.LottaRewards.com 2017-01-22T19:00:34Z LottaRewards.com email@example.com Powerball And Mega Millions Weekend Lotto Results 22 Jan 2017 2017-01-22T19:00:34Z firstname.lastname@example.com email@example.com PostId_9796 Both American lotteries are showing their muscle again with US jackpot climbing to an incredible $170 million for this Wednesday's draw and Mega Millions blissfully soaring to an unbelievable $177 million up for grabs this Tuesday. Friday night's Mega Millions draw didn't go as smoothly as usual though, but to find out what happened you will need to read further… Powerball Soars To 153 Million Dollars 2017-01-19T09:58:41Z email@example.com email@example.com PostId_9786 The US Powerball jackpot craze is officially here folks with the jackpot rolling over again to a jaw-dropping $153 million! 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Tonight's The Night - Win $359M On Powerball 2016-11-23T13:30:28Z firstname.lastname@example.com email@example.com PostId_9522 Tonight's the night! It's Wednesday, and that means life as you know it could change forever if you play Powerball online and win the massive $359 million up for grabs. Lucky Mega Millions Ticket Scoops $82m 2016-11-21T05:55:55Z email@example.com email@example.com PostId_9510 One very lucky Mega Millions ticket sold in Georgia has scooped up the whole $82m jackpot in Friday's Mega Millions draw. Couple Denied Mortgage Win $44m NZ Powerball Days Later 2016-11-14T20:08:26Z firstname.lastname@example.com email@example.com PostId_9490 Just when we told you about the newlywed lovebirds who received a kick-start to the marriage from non-other than Lady Luck herself scooping a huge €500,000 EuroMillions win, we now hear about a couple who just after being denied a mortgage, win $44 million on the New Zealand Powerball jackpot. The US Powerball Continues Its Rollover To €293 Million 2016-11-14T18:06:01Z firstname.lastname@example.com firstname.lastname@example.com PostId_9489 The US Powerball top prize continues its rollover into triple digits after no one managed to match all the Powerball winning numbers on Saturday night. The Power of Powerball At $258M 2016-11-11T10:39:27Z firstname.lastname@example.com email@example.com PostId_9478 The world as a whole has a million ways to quantify power. We just have to look at horsepower, ergs per second, gigawatts, earthquakes and of course the mighty American Powerball which as once again increased its might rolling over to a massive $258 million. 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text: b. William Francis OGÇÖConnell, 22 August 1953, New York City, New York, USA. Raised in Port Washington, Long Island, OGÇÖConnell studied piano with his uncle, Bill Wisnewski, while still in school, then went on to study piano and composition at the Oberlin Conservatory of Music. He also studied for a year with Richie Beirach in New York City. From the late 70s and through the early 80s, he worked in sometimes-exalted company, including spending two years with Mongo Santamar+¡a ... Read more


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Magneto #0 (1993) Marvel Comics X-MenMagneto #0.Top Rated SellerC $1.30C $22.11 shippingFrom United States
1996 Magneto # 1 NM 9.4These have never been read. I try to make the best call that I can on the condition as I see the comic.Top Rated SellerC $3.93C $20.91 shippingFrom United States
Magnetic Men #1 Marvel DC Amalgam Comics Crossover MagnetoMagneto and The Magnetic Men #1.Top Rated SellerC $1.30C $21.19 shippingFrom United States
X-Men MAGNETO Elektra Plush Toy Doll Kellytoy 2003Excellent condition.Top Rated SellerC $13.14From United States
Magneto #3 2014 VF-NM10 =6.99.Top Rated SellerC $3.29C $21.56 shippingFrom United States
X-Men: Magneto - Testament #1-5 VF/NM complete series - greg pak - origin storyTHIS IS A ORIGIN MINI SERIES. BOOKS ARE VERY FINE TO NEAR MINT IN CONDOTION AND ALL COME BAGGED AND BOARDED.Top Rated SellerC $32.87From United StatesC $12.43 shipping
Magneto #0 & 1-4 VF/NM complete series PETER MILLIGAN kelley jones marvel x-menWriter: Peter Milligan. Peter Milligan. Item: Magneto #0 & 1-4. Artist: Kelley Jones. Kelley Jones. Publisher: Marvel Comics. Marvel Comics. Other Info: complete series!Top Rated SellerC $13.14From United StatesC $23.02 shipping
Magneto #19 2015 VF-NM10 =6.99.Top Rated SellerC $2.49C $21.54 shippingFrom United States
Marvel Comics Magneto # 0 - The Twisting of a Soul comicComic in great condition inside and out.C $2.62From United StatesC $20.87 shipping
Magnetic Men Featuring Magneto (1997) #1 VF"Born Again!". Story by Tom Peyer. Art by Barry Kitson and Dan Panosian. In order to save two universes, the young hero known as Access was forced to use the power of Amalgam (see Marvel versus DC (1996) #3).Top Rated SellerC $2.24C $21.04 shippingFrom United States
Magneto Not A Hero #1 Very FineJust hit buy it now on the ones you want and then request total.Top Rated SellerC $1.30C $22.33 shippingFrom United States
Magneto: Dark Seduction #4 (Sep 2000, Marvel) $2.99 CP - Mind-Blowing ConclusionCGC is a professional grader. All comics will be described as showing either heavy, medium, mild or minimal wear. He was about 15 years old at the time. Smaller orders of comics (typically 1-5) are slabbed with a sheet of cardboard cut to be comic book size, then placed inside a 10" x 13" plastic envelope.Top Rated SellerC $1.30C $20.83 shippingFrom United States
MAGNETO #3 - VF - Marvel Comics– • MAGNETO CUTS LOOSE! • At the source of the newest Sentinel threat, Magneto puts what's left of his magnetic powers to good use. • This may very well be the first step in Magneto becoming the VILLAIN he once was… "PROFILE OF A SUPREMACIST" • In the most brutal issue yet of this new noir/horror series, Magneto ruthlessly dismantles the latest threat to mutantkind… • …and we gain a greater understanding of why his mission is so important to him.Top Rated SellerC $1.58From United States
Deadpool #1 Signed and Sketched by Scott Koblish CGC Signature Series 9.8 GradedThe book is graded 9.8 via CGC's Signature Series. Scott Koblish sketched an awesome looking Deadpool holding up the head of a zombie. This is an awesome original piece for Deadpool fans. Great collectors and display piece.Top Rated SellerC $284.07C $16.55 shippingFrom United States11 watching11 watching
Magneto Rex #2 (Jun 1999, Marvel)Comic will come bagged and boarded.Top Rated SellerC $1.97From United StatesC $22.19 shipping
Magneto #14 March 2015 VF-NM10 =6.99.Top Rated SellerC $2.63C $21.54 shippingFrom United States
Magneto #20 September 2015 VF-NM10 =6.99.Top Rated SellerC $1.30C $21.52 shippingFrom United States
MAGNETO AND THE MAGNETIC MEN #1 ONE SHOT AMALGAM COMICS UNREAD NM+ OR BETTERAMALGAM COMICS. MAGNETO AND THE MAGNETIC MEN. ISSUE #1 - ONE SHOT.Top Rated SellerC $6.56C $29.92 shippingFrom United States
Magneto Rex #2 (Jun 1999, Marvel)Top Rated SellerC $1.96C $20.85 shippingFrom United States
MAGNETO #17 - NM - Marvel Comics• In the wake of AVENGERS & X-MEN: AXIS, Magneto has taken a group of wayward mutants into his care on the abandoned island of Genosha. • But now someone is hunting and killing those mutants one by one, right under his nose.Top Rated SellerC $2.10From United States
Magneto #8 Marvel Now! VF-NM10 =6.99.Top Rated SellerC $2.96C $21.56 shippingFrom United States
X-Men Magneto Testament (2008) #2 FNWritten by GREG PAK Art by CARMINE DI GIANDOMENICO Cover by MARKO DJURDJEVIC The latest from the Marvel Knights line! The definitive origin story of one of Marvel's greatest icons continues as young Magneto, a Jewish boy in Hitler's Germany, travels to Berlin with his father in a desperate attempt to secure protection from the Nazi menace.Top Rated SellerC $2.24C $21.04 shippingFrom United States
X-Men Magneto Testament (2008) #1 FNBut in 1935, he was just another schoolboy -- who happened to be Jewish in Nazi Germany. The definitive origin story of one of Marvel's greatest icons begins with a silver chain and a crush on a girl -- and quickly turns into a harrowing struggle for survival against the inexorable machinery of Hitler's Final Solution.Top Rated SellerC $2.24C $21.04 shippingFrom United States
MAGNETO #0 GOLD FOIL VARIANT RETAILER INCENTIVE COVER X-MEN MARVEL COMICSIncluding more Trade Paperbacks, Graphic Novels, Comics.Top Rated SellerC $52.59C $21.21 shippingFrom United States12 watching12 watching
Magneto #15 - (Marvel, 2015) - VF/NMUp for sale is Magneto #15 f rom Marvel Comics circa 2015. The estimated grade of this copy is VF/NM (see cover scans). This is an opportunity to own a copy of this issue at a reasonable price. The comic will come bagged and boarded.Top Rated SellerC $2.35Was: Previous PriceC $3.93From United StatesC $6.58 shipping


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In the present study, we investigated the role of Paeonia lactiflora Pall. extract on embryo implantation in vitro and in vivo. A polysaccharides depleted-water extract of P. lactiflora (PL-PP) increased LIF expression in human endometrial Ishikawa cells at non-cytotoxic doses. PL-PP significantly increased the adhesion of the human trophectoderm-derived JAr spheroids to endometrial Ishikawa cells. PL-PP-induced LIF expression was decreased in the presence of a p38 kinase inhibitor SB203580 and an MEK/ERK inhibitor U0126. Furthermore, endometrial LIF knockdown by shRNA reduced the expression of integrins β3 and β5 and adhesion of JAr spheroids to Ishikawa cells. In vivo administration of PL-PP restored the implantation of mouse blastocysts in a mifepristone-induced implantation failure mice model. Our results demonstrate that PL-PP increases LIF expression via the p38 and MEK/ERK pathways and favors trophoblast adhesion to endometrial cells.
Citation: Choi H-J, Chung T-W, Park M-J, Lee KS, Yoon Y, Kim HS, et al. (2016) Paeonia lactiflora Enhances the Adhesion of Trophoblast to the Endometrium via Induction of Leukemia Inhibitory Factor Expression. PLoS ONE 11(2): e0148232. doi:10.1371/journal.pone.0148232
Editor: Geetanjali Sachdeva, National Institute for Research in Reproductive Health, INDIA
Received: January 22, 2015; Accepted: January 14, 2016; Published: February 3, 2016
Copyright: © 2016 Choi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All relevant data are within the paper and its Supporting Information files.
Funding: This study was supported by a grant of the Traditional Korean Medicine R&D Project, Ministry of Health & Welfare (Grant no. HI13C0502), and by the National Research Foundation of Korea (NRF) grant funded by the Ministry of Science, ICT & Future Planning (MISP), Korea Government (Grant no. 2014R1A5A20009936).
Competing interests: The authors have declared that no competing interests exist.
Implantation is a complex biological process, requiring communication between the appropriately developed trophoblast and the receptive endometrium [1]. This process is established and maintained by diverse biological factors, including cytokines, growth factors, and receptors [2]. Embryo implantation is adversely affected by abnormal expression of the genes related to the establishment of uterine receptivity [3]. Among these factors, leukemia inhibitory factor (LIF) plays a key role in determining the outcome of implantation [4–6]. Genetic mutations and aberrant expression of LIF are known to contribute to implantation failure in mice and humans [7,8]. In addition, inhibition of LIF activity by addition of an anti-LIF antibody or LIF antagonist effectively prevents embryo implantation in mice, monkeys, and humans [9–12].
In recent years significant advances have been made in assisted reproductive technologies (ART). However, pregnancy rates remain low [13]. High doses of exogenous gonadotropins used for ovarian stimulation during in vitro fertilization (IVF) procedures are known to impair endometrial receptivity [14]. Traditional herbal remedies and acupuncture, therefore are being proposed as alternative treatments for improving endometrial receptivity [15–17]. Several groups have reported that LIF is an important molecular target of herbal remedies and acupuncture [18–20].
The roots of P. lactiflora Pall., belonging to the Paeoniaceae family, have been used as a therapeutic drug for treating fever, rheumatoid arthritis, systemic lupus erythematosus, hepatitis, and gynecological disorders in Korea, Japan, and China [21,22]. Especially, P. lactiflora has been used for several years to treat various gynecological problems such as dysmenorrhea, cramps and spasms during pregnancy, and infertility [23,24]. Different components extracted from the roots of P. lactiflora are reported to have anti-inflammatory, immunomodulatory, anti-allergic, anti-arthritic, and hepatoprotective activities [21,25–27]. However, the biological effects of P. lactiflora extract on endometrial receptivity have not been explored.
Therefore, in this study, we evaluated the effects of P. lactiflora extract on the LIF expression in endometrial Ishikawa cells and adhesion of trophoblastic JAr spheroids to Ishikawa cells. In addition, the effect of P. lactiflora extract on embryo implantation was examined in an implantation- failure mouse model.
Materials and Methods
Antibodies against LIF, extracellular signal-regulated kinase (ERK), p-ERK, p38, signal transducer and activator of transcription 3 (STAT3), p-STAT3, and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-p-p38 was obtained from Cell Signaling Technology (Danvers, MA, USA). Inhibitors of specific signaling pathways, including LY294002 (inhibitor for Phosphoinositide 3-Kinase; PI3K), U0126 (inhibitor for Mitogen-Extracellular signal-regulated Kinase; MEK/ERK), SB203580 (inhibitor for p38), and SP600125 (inhibitor for c-Jun N-terminal Kinase; JNK), were obtained from Merck Millipore (Billerica, MA, USA). An antagonist of the progesterone receptor, mifepristone (RU486), was purchased from Sigma-Aldrich (St. Louis, MO, USA).
Extract preparation
The roots of P. lactiflora were purchased from Omniherb Co. (Daegu, Korea). The herb was authenticated by a botanist at Omniherb Co. A voucher specimen (DC-H21) in the form of a frozen rhizome was deposited in the Korean Medicine Research Center for Healthy Aging, Pusan National University (Yangsan, Korea). The extraction method is described in S1 Fig. PL-WE and PL-PP were freshly dissolved in distilled water before performing subsequent experiments.
Fingerprinting high-performance liquid chromatography (HPLC) analysis
HPLC analysis was performed using a Shimadzu HPLC system (Shimadzu Co., Kyoto, Japan), consisting of a solvent delivery unit, an on-line degasser, a column oven, an autosampler, and an SPD-20A UV detector. For data analysis, LC solution software (Version 1.25) was used. The analytical column used was an ACE C18 column (4.6 × 250 mm i.d., 5 μm pore size). The mobile phases were solvent A (acetonitrile) and solvent B (0.1% phosphoric acid). The gradient flow was as follows: (A)/(B) = 10–15/90–85 (0–5 min) → (A)/(B) = 15–22/85–78 (5–25 min) → (A)/(B) = 22–70/78–30 (25–45 min) → (A)/(B) = 70–80/30–20 (45–46 min) → (A)/(B) = 80/20 (46–50 min). The column temperature was maintained at 25°C. The analysis was carried out at a flow rate of 1 mL/min with UV detection at 254 nm. The column injection volume was 20 μL. The standard solution and PL-PP were prepared by dissolution in dimethyl sulfoxide (DMSO; 1 mM and 50 mg/mL, respectively). The solutions were filtered through a 0.45-μm membrane filter (Millipore) and applied to HPLC.
Cell culture
Human endometrial Ishikawa cell line, derived from a human adenocarcinoma, was kindly provided by Dr. Jacques Simard (CHUL Research Center, Quebec, Canada). The cells were maintained as monolayers at 37°C in an atmosphere containing 5% CO2/air in Dulbecco's Modified Eagle Medium (DMEM; Welgene, Daegu, Korea) containing 10% heat-inactivated fetal bovine serum (FBS, Sigma-Aldrich) and 1% penicillin/streptomycin (Gibco, Rockville, MD, USA). The human choriocarcinoma JAr cells were obtained from the Korean Cell Line Bank (Seoul, Korea) and cultured in RPMI1640 (Welgene) containing 10% heat-inactivated FBS and 1% penicillin/streptomycin. AN3CA cell line was purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).
Cell viability Assay
The cytotoxic effects of PL-WE and PL-PP were examined using the methylthiazolyldiphenyl-tetrazolium bromide assay (MTT, Sigma-Aldrich). In brief, Ishikawa cells were cultured in 24-well plates with PL-WE and PL-PP (indicated concentrations) for 24 h. After removal of the culture medium, 300 μL of 1× MTT solution (0.5 mg/mL) was added to each well. After incubation for 4 h at 37°C in a CO2 incubator, the supernatants were removed and formazan crystals formed in viable cells were dissolved in 300 μL of ethanol:DMSO (v/v, 1:1). To detect the cytotoxicity, the absorbance in each well was measured at 540 nm with a microplate reader. The percentage of living cells in treated cultures was calculated relative to that in untreated cultures.
Reverse transcription-polymerase chain reaction (RT-PCR)
Total RNA was isolated from Ishikawa cells using RiboEx™ (GeneAll, Seoul, Korea). Equal amounts of total RNA (1 μg) from each sample were then subjected to reverse transcription with oligo-dT primers by using M-MLV reverse transcriptase (Enzynomics, Daejeon, Korea). The cDNA was amplified by PCR using DiaStar™ Taq DNA Polymerase (Solgent Co., Daejeon, Korea). The PCR conditions, amplified size of each target gene, and primers used in this study are shown in Table 1.
Western blot analysis
The total protein was isolated from Ishikawa cells exposed to PL-WE or PL-PP (indicated concentrations). Protein (20 μg) from each sample was boiled with 6× sodium dodecyl sulfate (SDS) sample loading buffer (0.35M Tris-HCl [pH 6.8], 10% SDS, 30% glycerol, 9.3% dithiothreitol [DTT], and 12% bromophenol blue), and then separated using 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel. The size-fractioned protein sample was electro-transferred to nitrocellulose membranes. The membranes were blocked using 5% blocking solution and incubated with anti-LIF, p-Erk, Erk, p-p38, p38, and anti-β-actin antibodies. After reaction with the appropriate secondary antibodies linked to horseradish peroxidase, signals were visualized using the ECL chemiluminescence system (GE Healthcare, Uppsala, Sweden).
LIF knockdown by shRNA
To knock down endogenous hLIF, lentiviral shRNA constructs were obtained from Open Biosystems (Thermo Scientific, Waltham, MA, USA). 293T cells (1.5 × 106) were subcultured in a 10 cm dish. Twenty-four hours after cell seeding, the lentiviral vector encoding hLIF shRNA (3 μg), packaging vector pCMV-VSVG (0.3 μg), and the envelop plasmid pHRCMV-8.2 ΔR (3 μg) were transfected in 293T cells using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). Forty-eight hours after transfection, the supernatant harboring lentiviruses was collected and filtered using a sterilized 0.45 μm syringe filter. LIF shRNA lentiviral supernatant with polybrene (5 μg/mL) was added to the Ishikawa cells. To select cells with stable integration of LIF shRNA, the viral infected Ishikawa cells were treated with 3 μg/mL puromycin for 1 week. The knockdown efficiency of the LIF shRNA was verified by western blot analysis. The best hLIF shRNA #5, among 5 hLIF shRNA clones (#3–#7), was used for subsequent experiments. The sequence of LIF shRNA #5 used for knocking down LIF was 5'-TTACCCGAGGTGTCAGGGCCG-3'.
JAr spheroid adhesion assay
Ishikawa cells (3 × 105 cells) were seeded into 24 well plates and cultured for 24 h. The medium was replaced and the cells were incubated in serum-free -DMEM containing PL-PP for 48 h. To make JAr spheroids, we used the GravityPLUS™ Kit (InSphero Co.) according to the manufacturer's manual. Briefly, after JAr cells were trypsinized and counted, the suspended JAr cells (1 × 103 /40 μl RPMI medium) were gently delivered into each well of the GravityPLUS™ plate and incubated in a humidified 5% CO2 incubator at 37°C for 3 days. JAr spheroids (20/well) were gently delivered onto a confluent monolayer of Ishikawa cells grown in a 24 well culture plate. After incubation for 2 h at 37°C, the cells were washed with 10% FBS/RPMI medium three times to remove non-binding JAr spheroids. The attached JAr spheroids were visualized using a camera and counted.
Male and female C57BL/6 mice (7–8 weeks old, weight 20–22 g), inbred in a specific pathogen-free (SPF) facility, were purchased from Orient Bio, Co. (Seongnam, Korea). They were bred separately and had free access to water and a standard diet with a 12 h light / 12 h dark cycle. All experimental procedures were examined and approved by the Animal Research Ethics Committee at the Pusan University of Korea.
Animal models and treatment
All 33 female mice were randomly divided into 3 groups, including a control group, RU486 group, and RU486 with PL-PP group. To improve implantation, the female mice were treated with PL-PP every day for 17 days. Female mice belonging to the RU486 with PL-PP group received PL-PP orally (1.06 mg/20 g) every day, while the mice belonging to the control and RU486 groups received the same amount of PBS. After seven days of daily treatment with PL-PP, all females were caged with males (ratio 2:1) at 6 pm and the day when vaginal plugs were detected was designated as day 1 of pregnancy. The embryo implantation failure model was developed according to previous reports with some modifications [20,28]. Briefly, the mice belonging to the RU486 group and RU486 with PL-PP group were injected subcutaneously with 0.08 mg/0.1 mL of RU486 solution, while the mice belonging to the control group were injected with the same volume of corn oil on day 4 of pregnancy at 9 am. Seven days after RU486 injection, all mice were euthanized by inhalation of CO2 gas and both uterine horns were excised to determine the number of implantation sites. The number of implanted embryos on each uterine horn was recorded.
Densitometry and statistical analysis
The intensity of the bands obtained from RT-PCR and western blot analysis, and the attached spheroids from the adhesion assay were quantified with ImageJ software. Data from all experiments are indicated as the percentage or fold compared to the control and expressed as the mean ± SD. The differences between the mean values of 2 groups were determined using a standard t-test with the GraphPad Prism (GraphPad Software, CA, USA). Furthermore, for comparison among groups, one-way analysis of variance (ANOVA) tests with a Dunnet's post-hoc comparison were used with the assistance of GraphPad Prism. The minimum significance level was set at a P value of 0.05. All experiments were independently performed at least 3 times.
Phytochemical validation of P. lactiflora extracts and effect on cell viability of Ishikawa cells
As shown in Fig 1, PL-PP contained gallic acid, catechin, methyl gallate, paeoniflorin, and benzoic acid. PL-PP contains 151.14 μg/mg (>15%) paeoniflorin, which was the most abundant molecule among the five compounds tested (S1 Table).
HPLC was performed as described in the Materials and Methods. Retention times of the standard compounds were 4.8, 10.5, 11.5, 17.5, and 28 min for gallic acid, catechin, methyl gallate, paeoniflorin, and benzoic acid, respectively.
As shown in S2A Fig, PL-WE treatment inhibited cell growth in a dose-dependent manner. Treatment with 10, 50, 100, and 500 μg/mL PL-WE showed 8, 22, 31, and 63% inhibition, respectively, while PL-PP treatment at concentrations of 500 μg/mL inhibited cell proliferation by 21% (S2B Fig). PL-PP did not have significant cytotoxic activity up to 100 μg/mL; therefore, we used PL-PP concentrations up to 50 μg/mL for the subsequent experiments.
PL-PP increases LIF expression in Ishikawa cells and adhesion of JAr spheroids to Ishikawa cells
Next, we investigated whether P. lactiflora extracts could induce LIF expression in Ishikawa cells. As shown in S3A and S3B Fig, LIF mRNA and protein levels were significantly increased by PL-PP treatment in Ishikawa cells. We also confirmed the increase in LIF protein expression in PL-PP-treated Ishikawa cells (S3B Fig). Ishikawa cells were treated with PL-PP at 10, 30, and 50 μg/mL doses. Both mRNA and protein levels increased in a dose-dependent manner (Fig 2A and 2B). We next examined whether PL-PP enhances the adhesion of Jar spheroids to Ishikawa cells. As shown in Fig 2C, the number of JAr spheroids adhered to PL-PP-treated Ishikawa cells (17.6/20, 88%) was significantly higher than that adhered to the control cells (8.6/20, 43%). PL-WE, on the other hand, did not affect the adhesion of JAr spheroids to Ishikawa cells (S4 Fig).
(A, B) Total RNA and protein were isolated after treatment with the indicated concentrations of PL-PP in serum—free medium for 24 h. Relative levels of LIF mRNA and protein were examined by RT-PCR and western blot analysis, respectively. The intensity of the band of interest was estimated by densitometric analysis and calculated as the mean ± SD of three independent experiments (* P < 0.05, ** P < 0.01, *** P < 0.001 compared to the control group). (C) Ishikawa cells were cultured in 24-well plates and treated with or without PL-PP (50 μg/mL) for 48 h. Twenty JAr spheroids were added onto the Ishikawa cell monolayer. The number of JAr spheroids bound to confluent Ishikawa cells was manually counted and calculated as the mean ± SD of three independent experiments (* P < 0.05 compared to each group).
PL-PP regulates LIF expression through the p38 and MEK/ERK signaling pathways
To determine the upstream signaling pathways responsible for PL-PP-induced LIF expression, we used various signaling inhibitors. The specific inhibitors of JNK (SP600125) and PI3K (LY294002) had no effect on the PL-PP-induced LIF expression (Fig 3A and 3B). However, p38MAPK (SB203580) and MEK/ERK inhibitors (U0126) did inhibit the PL-PP-induced LIF expression. As shown in Fig 3C, PL-PP markedly induced the phosphorylation of p38 and ERK. These results suggest that the activation of p38 and ERK, but not that of JNK or PI3K, is required for the PL-PP-induced expression of LIF.
Ishikawa cells were pre-treated with SP600125 (10 μM), LY294002 (15 μM), SB203580 (10 μM), and/or U0126 (0.5 μM) for 1 h. After treatment of each signaling inhibitor, the cells were stimulated with PL-PP (50 μg/mL) for 24 h. Total RNA and protein were isolated from each sample and LIF expression was evaluated by RT-PCR (A) and western blot analysis (B). The intensity of the band of interest was estimated by densitometric analysis and calculated as means ± SD of two independent experiments (# P < 0.01 vs. the untreated control. * P < 0.05 and ** P < 0.01 vs. the PL-PP-treated control). Ishikawa cells were treated with PL-PP for the indicated times (0, 0.5, 1, 2, and 4 h). Total protein was isolated from each sample and p38 and ERk phosphorylations were evaluated by western blot analysis (C). The intensity of the band of interest was estimated by densitometric analysis and calculated as means ± SD of three independent experiments (* P < 0.05 compared to each control).
LIF knockdown inhibits adhesion of JAr spheroids to PL-PP-treated Ishikawa cells
To examine whether LIF plays an important role in promoting the adhesion of JAr spheroids to PL-PP-treated Ishikawa cells, we knocked down LIF expression in Ishikawa cells by shLIF lentiviral infection. The efficiency of knock-down was evaluated by LIF expression using RT-PCR (data not shown). Of STAT3, a down-stream effector of LIF signaling was found to be not activated by PL-PP treatment in LIF-knockdown cells (S5 Fig). As shown in Fig 4, the adhesion of JAr spheroids to PL-PP treated LIF-knockdown Ishikawa cells was significantly (10.25/20, 51, 25%) reduced compared to PL-PP-induced control Ishikawa cells (17.5/20, 87.5%). The data suggests that PL-PP-stimulated LIF gene expression may be necessary to facilitate the adhesion of Jar spheroids to Ishikawa cells.
Ishikawa cells harboring pLKO.1 or shLIF vector were cultured in 24-well plates and treated with or without PL-PP (50 μg/mL) in serum-free medium for 48 h. Twenty JAr spheroids were added onto the Ishikawa cell monolayer. Representative pictures were taken and the number of adhered JAr spheroids to Ishikawa cells was counted and calculated as the mean ± SD of three independent experiments (** P < 0.01 and *** P < 0.001 compared to each group).
PL-PP positively regulates the expression of adhesion molecules such as integrin β3 and β5 in LIF-dependent manner
We examined whether PL-PP has an effect on expression of various adhesion molecules using RT-PCR. As shown in Fig 5A, PL-PP induced the expression of integrin β3 (ITGB3) and ITGB5, but not of integrin αV (ITGAV), ITGB1, ITGB4, intercellular adhesion molecule 1 (ICAM-1), L-selectin, and CD44 in Ishikawa cells. A decrease was observed in the mRNA levels of E-cadherin on treatment of Ishikawa cells with PL-PP (Fig 5A). PL-PP markedly increased the expression of LIF and ITGB5 mRNA in non-receptive endometrial cell line- AN3-CA. However, the expression levels of other adhesion molecules such as integrin αV, β1, β3, β4, CD44, ICAM-1, L-selectin, and E-cadherin were not modulated by PL-PP treatment. PL-PP enhanced, thiugh not significantly, the adhesion of JAr spheroids to AN3-CA cells (S6 Fig).
(A) Ishikawa cells were treated with or without PL-PP (50 μg/mL) for 24 h, and total RNA was extracted. The expression levels of LIF, ITGAV, ITGB1, ITGB3, ITGB4, ITGB5, ICAM-1, L-selectin, E-cadherin, and CD44 mRNA were analysed by RT-PCR. β-actin was used as an internal control. (B) The pLKO.1 –or shLIF—transfected Ishikawa cells were treated with PL-PP (50 μg/mL) for 24 h. Total RNA was extracted and ITGB3 and ITGB5 mRNA expression levels were measured by RT-PCR. β-actin was used as an internal control.
To further investigate whether the PL-PP induced increase in the expression of adhesion molecules is mediated via a LIF-dependent pathway, we assessed ITGB3 and ITGB5 transcript levels in PL-PP treated pLKO.1- and shLIF-transfected Ishikawa cells. The transcript levels of ITGB3 and ITGB5 were found to be lower in PL-PP treated LIF knock-down cells compared with PL-PP treated pLKO.1-transfected cells (Fig 5B). These results suggest that PL-PP induces an increase in the expression of ITGB3 and ITGB5 through a LIF-dependent pathway.
PL-PP administration restores implantation in an implantation-failure mouse model
We next investigated whether the administration of PL-PP could effectively promotes blastocyst implantation in vivo. First, we assessed the effect of RU486, a well-known contraceptive drug, on LIF expression in human endometrial Ishikawa cells. RU486 treatment did not have any significant effect on LIF expression (data not shown). We also examined the in vitro effect of PL-PP on the adhesion of JAr spheroids to RU486 treated-Ishikawa cells. As shown in Fig 6A, the number of JAr spheroids adhered to RU486-pretreated Ishikawa cells increased from 41% to 61.66% after treatment with PL-PP. Further, the total number of implanted embryos in the RU486 with PL-PP group (3.36 ± 3.07), though lower than that in the control group (6.70 ± 2.26), was significantly higher than that in the RU486 group (0.18 ± 0.60). This indicated that PL-PP restores the implantation defect induced by RU486 treatment. Taken together, the data suggest that PL-PP-induced LIF expression plays a crucial role in the adhesion of Jar spheroids to the endometrium (Fig 7).
(A) Ishikawa cells in the absence or presence of RU486 (10 μM) were treated with or without PL-PP (50 μg/mL) in serum free medium for 48 h. Twenty JAr spheroids were added onto the Ishikawa cell monolayer. The number of adherent JAr spheroids to Ishikawa cells was counted in representative pictures and calculated as the mean ± SD of three independent experiments (* P < 0.05, *** P < 0.001 compared to each group and ns means no significance) (B) Female mice were treated with or without RU486 and PL-PP. After 7 days, all mice were euthanized and both uterine horns were excised to determine the number of implantation sites. Pictures of implanted embryos are shown. Arrows indicate implanted embryos. The number of implantation sites was counted and calculated as the mean ± SD of three independent experiments (** P < 0.05 and *** P < 0.001 compared to each group). (C) The uterin tissue sections were analyzed histologically after H&E staining. Embryo implantation sites are indicated by arrows.
P. lactiflora induces LIF expression through the activation of p38 and MEK/ERK pathways, leading to an increase in the trophoblast adhesion to the endometrium. Inhibition of LIF expression blocks the expression of adhesion molecules, such as integrin β3 and β5, and adhesion of the trophoblast to the endometrium.
Embryo quality and endometrial receptivity are crucial factors for successful implantation. In this study, we investigated the potential of medicinal herbs to improve uterine receptivity. Although P. lactiflora is one of the most well-known herbs for the treatment of diverse gynecological diseases [21,22], the effects of P. lactiflora on endometrial receptivity have not been investigated. PL-WE, which is extracted from the roots of P. lactiflora with boiling water, contains polysaccharides. It has been reported that polysaccharides cause an abnormal cellular immune response and interruption of cell-cell interactions [29,30]. In the present study, PL-WE did not induce LIF expression in the endometrium and was found to be cytotoxic. PL-PP was devoid of polysaccharides. Biochemical analysis suggests that paeoniflorin is one of the major compounds contributing to the effects of P. lactiflora.
Several factors such as integrins, interleukin-1 (IL-1), calcitonin, amphiregulin, epidermal growth factor (EGF), HB (heparin binding)-EGF, colony stimulating factor-1, LIF, mucins, trophinin/tastin, Hoxa-10, and cyclooxygenase (COX) contribute to endometrial receptivity [31]. LIF, a key factor for embryo implantation, is expressed in the glandular epithelial, luminal epithelial and stromal cells of the endometrium [5,32]. Our investigations demonstrated that in vitro PL-PP treatment increases the LIF expression and the adhesion of Jar spheroids to Ishikawa cells. Further LIF knockdown inhibited the adhesion of JAr spheroids to PL-PP-treated Ishikawa cells. These results suggest that an increase in the LIF expression in response to PL-PP favors the adhesion of JAr spheroids to Ishikawa cells.
Interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α) induce the phosphorylation of nuclear factor κB (NF-κB), which binds to the corresponding responsive elements in the LIF promoter region [33]. IL-1β stimulated LIF induction is mediated by MEK/ERK and NF-κB-pathways in normal articular human chondrocytes [34]. TNF-α is known to induce LIF expression through p38MAPK signaling pathway in bone marrow stromal cells from pediatric patients with myelodysplastic syndrome and neuronal retinal cells [35,36]. Our results showed involvement of p38 and MEK/ERK signaling pathways in PL-PP-induced LIF expression. It has been reported that the inhibition of LIF-induced STAT3 activation in the endometrium suppresses implantation [37]. We also observed that the PL-PP induced increase in LIF expression involves STAT3 activation.
Implantation is associated with modulation in the endometrial expression of several adhesion molecules, including integrins [38–40]. Integrins α2β1, α3β1, and α6β4 are reported to be constitutively expressed in Ishikawa cells. Further the expression of α1β1 and αvβ3 integrins is reported to be under hormonal regulation [41]. The cell surface expression of adhesion molecules determines the adhesiveness of JAr spheroids to endometrial cells [42]. Human endometrial RL95-2 cells, known as highly adhesive cells, express integrin α6, β1, and β4 subunits. On the other hand, non-receptive human endometrial AN3-CA cells lack β4 integrin subunit and show reduced expression of α6 and β1 integrin subunits. This results in lesser adhesion of JAr spheroids to endometrial AN3-CA cells [43].
E-cadherin is located on the plasma membrane of the epithelial cells and is critical for the maintenance of adherent junctions [44–46]. It has been reported that E-cadherin expression is reduced in the uterine epithelium during implantation in rodents [47]. This results in the reorganization of the adherent junctions between epithelial cells to facilitate blastocyst implantation [47]. Our data also demonstrated a reduction in E-cadherin expression in endometrial cells, following PL-PP treatment. Further the study demonstrated that PL-PP induces the expression of integrins β3 and β5 in a LIF-dependent manner.
Danielsson et al. showed that RU486 treatment immediately post ovulation reduces endometrial glandular LIF expression at the expected time of implantation [48]. Lalitkumar et al. reported that RU486 inhibits human blastocyst attachment to an endometrial three-dimensional cell model in vitro [49]. A previous report showed that various cytokines such as EGF, TGF-α, PDGF, and IL-1β are potent inducers of LIF expression in endometrial stromal cells [48]. However, steroid hormones such as estradiol and progesterone do not exert a regulatory effect on LIF expression in human endometrial cells [50]. We also did not observe any adverse effect of RU486 on LIF expression in Ishikawa cells. Also the number of JAr spheroids attached to Ishikawa cells did not change due to pre-treatment with RU486. PL-PP treatment also augmented the number of implanted embryos in an in vivo implantation failure model, developed using RU486. It is likely that a PL-PP induced increase in LIF expression does not involve the progesterone receptor signaling pathway.
PL-PP increases the LIF expression in Ishikawa cells through p38 MAPK and MEK/ERK signaling pathways in endometrial Ishikawa cells. The increase in LIF expression by PL-PP favors the adhesion of human trophoblastic JAr spheroids to Ishikawa cells. Also, the administration of PL-PP improves embryo implantation in RU486-treated mice. These results suggest that PL-PP may facilitate the adhesion of the blastocyst to the endometrium. PL-PP may be considered as a potential therapeutic herbal medicine for improving pregnancy rates.
Supporting Information
S1 Fig. Schematic diagram of the extraction procedure from the roots of P. lactiflora.
S2 Fig. Cytotoxic effect of PL-WE and PL-PP on Ishikawa cells.
Twenty-thousand cells were cultured in 12-well plates with the indicated concentrations of (A) PL-WE and (B) PL-PP. Cell viability was estimated 24 h after treatment using an MTT assay. Data represent the mean ± SD of three independent measurements (* P < 0.05 and *** P < 0.001 compared to the control group).
S3 Fig. Effects of PL-WE or PL-PP on LIF expression in Ishikawa cells.
(A, B) Ishikawa cells were treated with PL-WE or PL-PP (50μg/mL) in serum-free medium for 24 h. Total RNA and protein were extracted from PL-WE- or PL-PP-treated Ishikawa cells. LIF expression was measured by RT-PCR and western blot analysis. The intensity of the band of interest was estimated by densitometric analysis, and calculated as the mean ± SD of three independent experiments (** P < 0.01 compared to each group).
S4 Fig. Effect of PL-WE on the adhesion of JAr spheroids to Ishikawa cells.
Ishikawa cells were cultured in 6-well plates and treated with or without PL-WE (50 μg/mL) for 48h. Twenty JAr spheroids were added onto the Ishikawa cell monolayer. The number of JAr spheroids bound to confluent Ishikawa cells was manually counted, and calculated as the means ± SD of three independent experiments (* P < 0.05 compared to each group).
S5 Fig. Effect of PL-PP on the activation of STAT3 in Ishikawa cells.
Ishikawa cells transfected with pLKO.1 and shLIF were treated with or without PL-PP (50 μg/mL) in serum-free medium for 24 h. The levels of STAT3 and p-STAT3 proteins were measured by Western blot analysis. The intensity of the band of interest was estimated by densitometric analysis and calculated as the mean ± SD of three independent experiments (* P < 0.05 compared to each group).
S6 Fig. The expression levels of the adhesion molecules by PL-PP in non-receptive endometrial AN3-CA cells and the adhesion of JAr spheroids to PL-PP -treated AN3-CA cells.
(A) AN3-CA cells were treated with or without PL-PP (50 μg/mL) for 24 h, and total RNA was extracted. The expression levels of LIF, ITGAV, ITGB1, ITGB3, ITGB4, ITGB5, ICAM-1, L-selectin, E-cadherin and CD44 mRNA were examined by RT-PCR. β-actin was used as an internal control. (B) AN3-CA cells were cultured in 24-well plates and treated with or without PL-PP (50 μg/mL) in serum-free medium for 48 h. Twenty JAr spheroids were added onto the Ishikawa cell monolayer. The number of adherent JAr spheroids to Ishikawa cells was counted in representative pictures, and calculated as the mean ± SD of three independent experiments (** P < 0.01 compared to each group).
S1 Table. Quantitative phytochemical constituents of PL-PP.
This study was supported by a grant from the Traditional Korean Medicine R&D Project, Ministry of Health & Welfare (Grant no. HI13C0502), and by the National Research Foundation of Korea (NRF) grant funded by the Ministry of Science, ICT & Future Planning (MISP), Korea Government (Grant no. 2014R1A5A20009936).
Author Contributions
Conceived and designed the experiments: HJC TWC JHB KTH. Performed the experiments: HJC TWC MJP SOL KJK. Analyzed the data: KSL YY HSK JHB KTH. Contributed reagents/materials/analysis tools: HSK JHL SMK SOL JHB KTH. Wrote the paper: HJC TWC KTH.
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Craters greet motoristsIf one were to forget for a moment that Medavakkam Main Road provides access to Grand Southern Trunk Road, Southern Sector Inner Ring Road, Pallavaram-Thoraipakkam 200ft Radial Road and Velachery Main Road, the stretch would come across as ... The Hindu, 1 month ago
One kilometre of sheer horror - The Hindu, 1 month ago
28 images for grand trunk road
Chaos reigns on stretches of GST Road
The Grand Southern Trunk Road that connects the city's suburbs with various towns in the southern districts is becoming congested by the day with pedestrians and users of public transport suffering the most. This is due to the haphazard parking by ...The Hindu, 3 weeks ago
Angry residents protest on highway
Residents of Urapakkam, upset over the lack of power for a week, resorted to a protest on Grand Southern Trunk Road on Sunday. The protest, in which more than 200 people took part, crippled the movement of vehicles on the national highway. Traffic was ...The Hindu, 1 month ago
Stone causeway of Grand Trunk road discovered in Bihar
According to historians, the Uttarpath was reconstructed during Sher Shah Suri's reign in the 16th century from present-day Afghanistan to Bangladesh and was renamed GT road by the Britishers in the 17th century.DNA, 1 month ago ASI discovers stone causeway of Grand Trunk road in Bihar Financial Express Bangladesh, 1 month ago
Rain reveals 'river'
Rudyard Kipling might have found it befitting. A hidden part of the fabled Grand Trunk Road that the English chronicler had described as a "river of life" has been brought back to light by the aqueous lifeline called monsoon. Archaeologists have discovered a ...The Telegraph, 1 month ago
Young man's murder sparks protest
PESHAWAR: A large number of people blocked the Grand Trunk Road on Sunday to protest the murder of a young man over a petty issue in Haider Colony here. The protesters placed the body on the road and demanded of the government to arrest the ...DAWN Group, 1 week ago
All's not well in Odisha's Grand Trunk corridors
BHUBANESWAR: Months after the deaths of elephants in the state, officials of the forest department stumble upon their carcasses. The cause of their deaths remains unclear months later. Altogether 46 elephants died in a year between 2000 and 2010 ...Times of India, 2 months ago
The Chevrolet Corvette Grand Sport takes on the Jaguar F-Type SVR video - Roadshow
[MUSIC] [SOUND] [MUSIC] Welcome to another Road Show shootout, where today we're going to have America's latest Corvette against Britain's latest quick cat. This is the Corvette Grand Sport, a lightweight special edition that's track optimized ...CNET News, 4 weeks ago
Canada's grand western loop around British Columbia is prime for a road trip
The far western portion of Canada's 1,840-mile Yellowhead Highway includes a stunning 90-mile drive along the Skeena River past the Coast Mountains. (Julia Duin) Everyone loves a grand circle, and this was mine: A 2,200-mile loop through British ...Washington Post, 2 months ago A perfect circle Oman Tribune, 1 month ago A perfect circle around British Columbia IOL, 1 month ago
PHOTO GALLERY: Brockville rings in 2017
He was on Grand Trunk time, but it was good enough to ring in 2017. Canada's first prime minister, Sir John A. Macdonald, a.k.a. local historian Brian Porter, made his long-awaited return just in time to usher in Canada's sesquicentennial year as ...Recorder and Times, 2 weeks ago
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