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AB5675 | Anti-Metabotropic Glutamate Receptor 5 Antibody, pain
Anti-Metabotropic Glutamate Receptor 5 Antibody, pain is an antibody against Metabotropic Glutamate Receptor 5 for use in IH & WB.
|Replacement Information
Special Offers
Key Specifications Table
|Species Reactivity
|Key Applications
|Antibody Type
|Affinity Purified
|Polyclonal Antibody
|Product Information
|Affinity Purified
|Purified rabbit polyclonal antibody in buffer containing PBS with 0.05% sodium azide.
|Physicochemical Information
|Materials Information
|Toxicological Information
|Safety Information according to GHS
|Safety Information
|Storage and Shipping Information
|Storage Conditions
|Stable for 1 year at 2-8°C from date of receipt.
|Packaging Information
|Material Size
|50 µg
|Transport Information
|Supplemental Information
Certificates of Analysis
References | 19 Available | See All References
|Reference overview
|Pub Med ID
|Posttraumatic stress disorder-like induction elevates β-amyloid levels, which directly activates corticotropin-releasing factor neurons to exacerbate stress responses.
Justice, NJ; Huang, L; Tian, JB; Cole, A; Pruski, M; Hunt, AJ; Flores, R; Zhu, MX; Arenkiel, BR; Zheng, H
The Journal of neuroscience : the official journal of the Society for Neuroscience 35 2612-23 2015
Show Abstract
Recent studies have found that those who suffer from posttraumatic stress disorder (PTSD) are more likely to experience dementia as they age, most often Alzheimer's disease (AD). These findings suggest that the symptoms of PTSD might have an exacerbating effect on AD progression. AD and PTSD might also share common susceptibility factors such that those who experience trauma-induced disease were already more likely to succumb to dementia with age. Here, we explored these two hypotheses using a mouse model of PTSD in wild-type and AD model animals. We found that expression of human familial AD mutations in amyloid precursor protein and presenilin 1 leads to sensitivity to trauma-induced PTSD-like changes in behavioral and endocrine stress responses. PTSD-like induction, in turn, chronically elevates levels of CSF β-amyloid (Aβ), exacerbating ongoing AD pathogenesis. We show that PTSD-like induction and Aβ elevation are dependent on corticotropin-releasing factor (CRF) receptor 1 signaling and an intact hypothalamic-pituitary-adrenal axis. Furthermore, we show that Aβ species can hyperexcite CRF neurons, providing a mechanism by which Aβ influences stress-related symptoms and PTSD-like phenotypes. Consistent with Aβ causing excitability of the stress circuitry, we attenuate PTSD-like phenotypes in vivo by lowering Aβ levels during PTSD-like trauma exposure. Together, these data demonstrate that exposure to PTSD-like trauma can drive AD pathogenesis, which directly perturbs CRF signaling, thereby enhancing chronic PTSD symptoms while increasing risk for AD-related dementia.
|Increased response to glutamate in small diameter dorsal root ganglion neurons after sciatic nerve injury.
Gong, K; Kung, LH; Magni, G; Bhargava, A; Jasmin, L
PloS one 9 e95491 2014
Show Abstract
Glutamate in the peripheral nervous system is involved in neuropathic pain, yet we know little how nerve injury alters responses to this neurotransmitter in primary sensory neurons. We recorded neuronal responses from the ex-vivo preparations of the dorsal root ganglia (DRG) one week following a chronic constriction injury (CCI) of the sciatic nerve in adult rats. We found that small diameter DRG neurons (less than 30 µm) exhibited increased excitability that was associated with decreased membrane threshold and rheobase, whereas responses in large diameter neurons (greater than 30 µm) were unaffected. Puff application of either glutamate, or the selective ionotropic glutamate receptor agonists alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and kainic acid (KA), or the group I metabotropic receptor (mGluR) agonist (S)-3,5-dihydroxyphenylglycine (DHPG), induced larger inward currents in CCI DRGs compared to those from uninjured rats. N-methyl-D-aspartate (NMDA)-induced currents were unchanged. In addition to larger inward currents following CCI, a greater number of neurons responded to glutamate, AMPA, NMDA, and DHPG, but not to KA. Western blot analysis of the DRGs revealed that CCI resulted in a 35% increase in GluA1 and a 60% decrease in GluA2, the AMPA receptor subunits, compared to uninjured controls. mGluR1 receptor expression increased by 60% in the membrane fraction, whereas mGluR5 receptor subunit expression remained unchanged after CCI. These results show that following nerve injury, small diameter DRG neurons, many of which are nociceptive, have increased excitability and an increased response to glutamate that is associated with changes in receptor expression at the neuronal membrane. Our findings provide further evidence that glutamatergic transmission in the periphery plays a role in nociception.
|Therapeutic molecules and endogenous ligands regulate the interaction between brain cellular prion protein (PrPC) and metabotropic glutamate receptor 5 (mGluR5).
Haas, LT; Kostylev, MA; Strittmatter, SM
The Journal of biological chemistry 289 28460-77 2014
Show Abstract
Soluble Amyloid-β oligomers (Aβo) can trigger Alzheimer disease (AD) pathophysiology by binding to cell surface cellular prion protein (PrP(C)). PrP(C) interacts physically with metabotropic glutamate receptor 5 (mGluR5), and this interaction controls the transmission of neurotoxic signals to intracellular substrates. Because the interruption of the signal transduction from PrP(C) to mGluR5 has therapeutic potential for AD, we developed assays to explore the effect of endogenous ligands, agonists/antagonists, and antibodies on the interaction between PrP(C) and mGluR5 in cell lines and mouse brain. We show that the PrP(C) segment of amino acids 91-153 mediates the interaction with mGluR5. Agonists of mGluR5 increase the mGluR5-PrP(C) interaction, whereas mGluR5 antagonists suppress protein association. Synthetic Aβo promotes the protein interaction in mouse brain and transfected HEK-293 cell membrane preparations. The interaction of PrP(C) and mGluR5 is enhanced dramatically in the brains of familial AD transgenic model mice. In brain homogenates with Aβo, the interaction of PrP(C) and mGluR5 is reversed by mGluR5-directed antagonists or antibodies directed against the PrP(C) segment of amino acids 91-153. Silent allosteric modulators of mGluR5 do not alter Glu or basal mGluR5 activity, but they disrupt the Aβo-induced interaction of mGluR5 with PrP(C). The assays described here have the potential to identify and develop new compounds that inhibit the interaction of PrP(C) and mGluR5, which plays a pivotal role in the pathogenesis of Alzheimer disease by transmitting the signal from extracellular Aβo into the cytosol.
|Time-dependent Gene Profiling Indicates the Presence of Different Phases for Ischemia/Reperfusion Injury in Retina.
Andreeva, K; Zhang, M; Fan, W; Li, X; Chen, Y; Rebolledo-Mendez, JD; Cooper, NG
Ophthalmology and eye diseases 6 43-54 2014
Show Abstract
Ischemia/reperfusion (IR) injury has been associated with several retinal pathologies, and a few genes/gene products have been linked to IR injury. However, the big picture of temporal changes, regarding the affected gene networks, pathways, and processes remains to be determined. The purpose of the present study was to investigate initial, intermediate, and later stages to characterize the etiology of IR injury in terms of the pathways affected over time. Analyses indicated that at the initial stage, 0-hour reperfusion following the ischemic period, the ischemia-associated genes were related to changes in metabolism. In contrast, at the 24-hour time point, the signature events in reperfusion injury include enhanced inflammatory and immune responses as well as cell death indicating that this would be a critical period for the development of any interventional therapeutic strategies. Genes in the signal transduction pathways, particularly transmitter receptors, are downregulated at this time. Activation of the complement system pathway clearly plays an important role in the later stages of reperfusion injury. Together, these results demonstrate that the etiology of injury related to IR is characterized by the appearance of specific patterns of gene expression at any given time point during retinal IR injury. These results indicate that evaluation of treatment strategies with respect to time is very critical.
|Synaptic depression via mGluR1 positive allosteric modulation suppresses cue-induced cocaine craving.
Loweth, JA; Scheyer, AF; Milovanovic, M; LaCrosse, AL; Flores-Barrera, E; Werner, CT; Li, X; Ford, KA; Le, T; Olive, MF; Szumlinski, KK; Tseng, KY; Wolf, ME
Nature neuroscience 17 73-80 2014
Show Abstract
Cue-induced cocaine craving is a major cause of relapse in abstinent addicts. In rats, cue-induced craving progressively intensifies (incubates) during withdrawal from extended-access cocaine self-administration. After ~1 month of withdrawal, incubated craving is mediated by Ca(2+)-permeable AMPA receptors (CP-AMPARs) that accumulate in the nucleus accumbens (NAc). We found that decreased mGluR1 surface expression in the NAc preceded and enabled CP-AMPAR accumulation. Thus, restoring mGluR1 transmission by administering repeated injections of an mGluR1 positive allosteric modulator (PAM) prevented CP-AMPAR accumulation and incubation, whereas blocking mGluR1 transmission at even earlier withdrawal times accelerated CP-AMPAR accumulation. In studies conducted after prolonged withdrawal, when CP-AMPAR levels and cue-induced craving are high, we found that systemic administration of an mGluR1 PAM attenuated the expression of incubated craving by reducing CP-AMPAR transmission in the NAc to control levels. These results suggest a strategy in which recovering addicts could use a systemically active compound to protect against cue-induced relapse.
|Hippocampal metaplasticity is required for the formation of temporal associative memories.
Xu, J; Antion, MD; Nomura, T; Kraniotis, S; Zhu, Y; Contractor, A
The Journal of neuroscience : the official journal of the Society for Neuroscience 34 16762-73 2014
Show Abstract
Metaplasticity regulates the threshold for modification of synaptic strength and is an important regulator of learning rules; however, it is not known whether these cellular mechanisms for homeostatic regulation of synapses contribute to particular forms of learning. Conditional ablation of mGluR5 in CA1 pyramidal neurons resulted in the inability of low-frequency trains of afferent activation to prime synapses for subsequent theta burst potentiation. Priming-induced metaplasticity requires mGluR5-mediated mobilization of endocannabinoids during the priming train to induce long-term depression of inhibition (I-LTD). Mice lacking priming-induced plasticity had no deficit in spatial reference memory tasks, but were impaired in an associative task with a temporal component. Conversely, enhancing endocannabinoid signaling facilitated temporal associative memory acquisition and, after training animals in these tasks, ex vivo I-LTD was partially occluded and theta burst LTP was enhanced. Together, these results suggest a link between metaplasticity mechanisms in the hippocampus and the formation of temporal associative memories.
|SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties.
Han, K; Holder, JL; Schaaf, CP; Lu, H; Chen, H; Kang, H; Tang, J; Wu, Z; Hao, S; Cheung, SW; Yu, P; Sun, H; Breman, AM; Patel, A; Lu, HC; Zoghbi, HY
Nature 503 72-7 2013
Show Abstract
Mutations in SHANK3 and large duplications of the region spanning SHANK3 both cause a spectrum of neuropsychiatric disorders, indicating that proper SHANK3 dosage is critical for normal brain function. However, SHANK3 overexpression per se has not been established as a cause of human disorders because 22q13 duplications involve several genes. Here we report that Shank3 transgenic mice modelling a human SHANK3 duplication exhibit manic-like behaviour and seizures consistent with synaptic excitatory/inhibitory imbalance. We also identified two patients with hyperkinetic disorders carrying the smallest SHANK3-spanning duplications reported so far. These findings indicate that SHANK3 overexpression causes a hyperkinetic neuropsychiatric disorder. To probe the mechanism underlying the phenotype, we generated a Shank3 in vivo interactome and found that Shank3 directly interacts with the Arp2/3 complex to increase F-actin levels in Shank3 transgenic mice. The mood-stabilizing drug valproate, but not lithium, rescues the manic-like behaviour of Shank3 transgenic mice raising the possibility that this hyperkinetic disorder has a unique pharmacogenetic profile.
|Western Blotting
|Amyloid-β and Alzheimer's disease type pathology differentially affects the calcium signalling toolkit in astrocytes from different brain regions.
Grolla, AA; Sim, JA; Lim, D; Rodriguez, JJ; Genazzani, AA; Verkhratsky, A
Cell death & disease 4 e623 2013
Show Abstract
The entorhinal-hippocampal circuit is severely affected in Alzheimer's disease (AD). Here, we demonstrate that amyloid-β (Aβ) differentially affects primary cultured astrocytes derived from the entorhinal cortex (EC) and from the hippocampus from non-transgenic controls and 3xTg-AD transgenic mice. Exposure to 100 nM of Aβ resulted in increased expression of the metabotropic glutamate receptor type 5 (mGluR5) and its downstream InsP3 receptor type 1 (InsP3R1) in hippocampal but not in EC astrocytes. Amplitudes of Ca(2+) responses to an mGluR5 agonist, DHPG, and to ATP, another metabotropic agonist coupled to InsP3Rs, were significantly increased in Aβ-treated hippocampal but not in EC astrocytes. Previously we demonstrated that senile plaque formation in 3xTg-AD mice triggers astrogliosis in hippocampal but not in EC astrocytes. The different sensitivities of the Ca(2+) signalling toolkit of EC versus hippocampal astrocytes to Aβ may account for the lack of astrogliosis in the EC, which in turn can explain the higher vulnerability of this region to AD.
|MeCP2 phosphorylation is required for modulating synaptic scaling through mGluR5.
Zhong, X; Li, H; Chang, Q
The Journal of neuroscience : the official journal of the Society for Neuroscience 32 12841-7 2012
Show Abstract
MeCP2 (methyl CpG binding protein 2) is a key player in recognizing methylated DNA and interpreting the epigenetic information encoded in different DNA methylation patterns. The functional significance of MeCP2 to the mammalian nervous system is highlighted by the discovery that mutations in the MECP2 gene cause Rett syndrome (RTT), a devastating neurological disease that shares many features with autism. Synaptic scaling is a form of non-Hebbian homeostatic plasticity that allows neurons to regulate overall excitability in response to changes in network neuronal activity levels. While it is known that neuronal activity can induce phosphorylation of MeCP2 and that MeCP2 can regulate synaptic scaling, the molecular link between MeCP2 phosphorylation and synaptic scaling remains undefined. We show here that MeCP2 phosphorylation is specifically required for bicuculline-induced synaptic scaling down in mouse hippocampal neurons and this phenotype is mediated by mGluR5 (metabotropic glutamate receptor 5). Our results reveal an important function of MeCP2 in regulating neuronal homeostasis and may eventually help us understand how MECP2 mutations cause RTT.
|β-Estradiol unmasks metabotropic receptor-mediated metaplasticity of NMDA receptor transmission in the female rat dentate gyrus.
Nebieridze, N; Zhang, XL; Chachua, T; Velíšek, L; Stanton, PK; Velíšková, J
Psychoneuroendocrinology 37 1845-54 2012
Show Abstract
Loss of estrogen in women following menopause is associated with increased risk for cognitive decline, dementia and depression, all of which can be prevented by estradiol replacement. The dentate gyrus plays an important role in cognition, learning and memory. The gatekeeping function of the dentate gyrus to filter incoming activity into the hippocampus is modulated by estradiol in a frequency-dependent manner and involves activation of metabotropic glutamate receptors (mGluR). In the present study, we investigated whether estradiol (EB) modulates the metaplastic effect of inducing synaptic long-term potentiation (LTP) on subsequent propensity for expression of LTP in the dentate gyrus. At medial perforant path-dentate granule cell synapses in hippocampal slices of ovariectomized female rats, EB replacement was critical for an initial induction of LTP to enhance the magnitude of subsequent LTP elicited by a second high-frequency stimulation, metaplasticity, which was not present in slices from oil-treated control animals. EB enhanced expression of group I mGluRs, and the metaplastic effect of EB on LTP required activation of group I mGluRs that led to Src-family tyrosine kinase-mediated phosphorylation of NR2B subunits of N-methyl-d-aspartate receptors (NMDAR) that enhanced the magnitude of NMDAR-dependent LTP. Our data show that EB effects on LTP in the hippocampal dentate gyrus require activation of group I mGluRs, which in turn leads to functional metaplastic regulation of NR2B subunit-containing NMDARs, as opposed to direct effects of EB on NMDARs.
|Metabotropic glutamate receptor 1 (mGluR1): antibody specificity and receptor expression in cultured primary neurons.
Ramses Ayala,Lauren R Kett,Tiffany L Leach,Anne B Young,Anthone W Dunah,Lianna R Orlando
Journal of neuroscience methods 204 2012
Show Abstract
The availability of high quality, well-characterized antibodies for molecular and cellular neuroscience studies is important. However, not all available antibodies are rigorously evaluated, nor are limitations of particular antibodies often reported. We have examined a panel of currently available mGluR1 antibodies and have identified which ones are selective for use by western blots and immunocytochemistry. We have also specifically determined whether the antibodies cross-react to recognize mGluR5, by examining (1) tissue from both mGluR1 and mGluR5 knock-out mice and (2) primary cortical cultures, in which mGluR5 is widely expressed but mGluR1 is not. Together, these data provide a baseline characterization of antibodies that can and cannot be reliably used in these types of studies, and will hopefully facilitate and positively impact the research efforts of others studying mGluR1.
|Uncoupling of the endocannabinoid signalling complex in a mouse model of fragile X syndrome.
Jung, KM; Sepers, M; Henstridge, CM; Lassalle, O; Neuhofer, D; Martin, H; Ginger, M; Frick, A; DiPatrizio, NV; Mackie, K; Katona, I; Piomelli, D; Manzoni, OJ
Nature communications 3 1080 2012
Show Abstract
Fragile X syndrome, the most commonly known genetic cause of autism, is due to loss of the fragile X mental retardation protein, which regulates signal transduction at metabotropic glutamate receptor-5 in the brain. Fragile X mental retardation protein deletion in mice enhances metabotropic glutamate receptor-5-dependent long-term depression in the hippocampus and cerebellum. Here we show that a distinct type of metabotropic glutamate receptor-5-dependent long-term depression at excitatory synapses of the ventral striatum and prefrontal cortex, which is mediated by the endocannabinoid 2-arachidonoyl-sn-glycerol, is absent in fragile X mental retardation protein-null mice. In these mutants, the macromolecular complex that links metabotropic glutamate receptor-5 to the 2-arachidonoyl-sn-glycerol-producing enzyme, diacylglycerol lipase-α (endocannabinoid signalosome), is disrupted and metabotropic glutamate receptor-5-dependent 2-arachidonoyl-sn-glycerol formation is compromised. These changes are accompanied by impaired endocannabinoid-dependent long-term depression. Pharmacological enhancement of 2-arachidonoyl-sn-glycerol signalling normalizes this synaptic defect and corrects behavioural abnormalities in fragile X mental retardation protein-deficient mice. The results identify the endocannabinoid signalosome as a molecular substrate for fragile X syndrome, which might be targeted by therapy.
|Reduced metabotropic glutamate receptor 5 density in major depression determined by [(11)C]ABP688 PET and postmortem study.
Deschwanden, A; Karolewicz, B; Feyissa, AM; Treyer, V; Ametamey, SM; Johayem, A; Burger, C; Auberson, YP; Sovago, J; Stockmeier, CA; Buck, A; Hasler, G
The American journal of psychiatry 168 727-34 2011
Show Abstract
Clinical and preclinical evidence suggests a hyperactive glutamatergic system in clinical depression. Recently, the metabotropic glutamate receptor 5 (mGluR5) has been proposed as an attractive target for novel therapeutic approaches to depression. The goal of this study was to compare mGluR5 binding (in a positron emission tomography [PET] study) and mGluR5 protein expression (in a postmortem study) between individuals with major depressive disorder and psychiatrically healthy comparison subjects.Images of mGluR5 receptor binding were acquired using PET with [(11)C]ABP688, which binds to an allosteric site with high specificity, in 11 unmedicated individuals with major depression and 11 matched healthy comparison subjects. The amount of mGluR5 protein was investigated using Western blot in postmortem brain samples of 15 depressed individuals and 15 matched comparison subjects.The PET study revealed lower levels of regional mGluR5 binding in the prefrontal cortex, the cingulate cortex, the insula, the thalamus, and the hippocampus in the depression group relative to the comparison group. Severity of depression was negatively correlated with mGluR5 binding in the hippocampus. The postmortem study showed lower levels of mGluR5 protein expression in the prefrontal cortex (Brodmann's area 10) in the depression group relative to the comparison group, while prefrontal mGluR1 protein expression did not differ between groups.The lower levels of mGluR5 binding observed in the depression group are consonant with the lower levels of protein expression in brain tissue in the postmortem depression group. Thus, both studies suggest that basal or compensatory changes in excitatory neurotransmission play roles in the pathophysiology of major depression.
|Chronic corticosterone administration down-regulates metabotropic glutamate receptor 5 protein expression in the rat hippocampus.
Iyo, AH; Feyissa, AM; Chandran, A; Austin, MC; Regunathan, S; Karolewicz, B
Neuroscience 169 1567-74 2010
Show Abstract
Several lines of evidence suggest a dysfunctional glutamate system in major depressive disorder (MDD). Recently, we reported reduced levels of metabotropic glutamate receptor subtype 5 (mGluR5) in postmortem brains in MDD, however the neurobiological mechanisms that induce these abnormalities are unclear. In the present study, we examined the effect of chronic corticosterone (CORT) administration on the expression of mGluR5 protein and mRNA in the rat frontal cortex and hippocampus. Rats were injected with CORT (40 mg/kg s.c.) or vehicled once daily for 21 days. The expression of mGluR5 protein and mRNA was assessed by Western blotting and quantitative real-time PCR (qPCR). In addition, mGluR1 protein was measured in the same animals. The results revealed that while there was a significant reduction (-27%, P=0.0006) in mGluR5 protein expression in the hippocampus from CORT treated rats, mRNA levels were unchanged. Also unchanged were mGluR5 mRNA and protein levels in the frontal cortex and mGluR1 protein levels in both brain regions. Our findings provide the first evidence that chronic CORT exposure regulates the expression of mGluR5 and are in line with previous postmortem and imaging studies showing reduced mGluR5 in MDD. Our findings suggest that elevated levels of glucocorticoids may contribute to impairments in glutamate neurotransmission in MDD.Full Text Article
|Western Blotting
|Assessment of the potential role of muscle spindle mechanoreceptor afferents in chronic muscle pain in the rat masseter muscle.
Lund, JP; Sadeghi, S; Athanassiadis, T; Caram Salas, N; Auclair, F; Thivierge, B; Arsenault, I; Rompré, P; Westberg, KG; Kolta, A
PloS one 5 e11131 2010
Show Abstract
The phenotype of large diameter sensory afferent neurons changes in several models of neuropathic pain. We asked if similar changes also occur in "functional" pain syndromes.Acidic saline (AS, pH 4.0) injections into the masseter muscle were used to induce persistent myalgia. Controls received saline at pH 7.2. Nocifensive responses of Experimental rats to applications of Von Frey Filaments to the masseters were above control levels 1-38 days post-injection. This effect was bilateral. Expression of c-Fos in the Trigeminal Mesencephalic Nucleus (NVmes), which contains the somata of masseter muscle spindle afferents (MSA), was above baseline levels 1 and 4 days after AS. The resting membrane potentials of neurons exposed to AS (n = 167) were hyperpolarized when compared to their control counterparts (n = 141), as were their thresholds for firing, high frequency membrane oscillations (HFMO), bursting, inward and outward rectification. The amplitude of HFMO was increased and spontaneous ectopic firing occurred in 10% of acid-exposed neurons, but never in Controls. These changes appeared within the same time frame as the observed nocifensive behaviour. Ectopic action potentials can travel centrally, but also antidromically to the peripheral terminals of MSA where they could cause neurotransmitter release and activation of adjacent fibre terminals. Using immunohistochemistry, we confirmed that annulospiral endings of masseter MSA express the glutamate vesicular transporter VGLUT1, indicating that they can release glutamate. Many capsules also contained fine fibers that were labelled by markers associated with nociceptors (calcitonin gene-related peptide, Substance P, P2X3 receptors and TRPV1 receptors) and that expressed the metabotropic glutamate receptor, mGluR5. Antagonists of glutamatergic receptors given together with the 2(nd) injection of AS prevented the hypersensitivity observed bilaterally but were ineffective if given contralaterally.Low pH leads to changes in several electrical properties of MSA, including initiation of ectopic action potentials which could propagate centrally but could also invade the peripheral endings causing glutamate release and activation of nearby nociceptors within the spindle capsule. This peripheral drive could contribute both to the transition to, and maintenance of, persistent muscle pain as seen in some "functional" pain syndromes.
|Differential role for synaptojanin 1 in rod and cone photoreceptors.
Lars C Holzhausen,Alaron A Lewis,Kimberly K Cheong,Susan E Brockerhoff
The Journal of comparative neurology 517 2009
Show Abstract
Synaptojanin 1 (SynJ1) is a polyphosphoinositide phosphatase involved in clathrin-mediated endocytosis in conventional synapses. Studies with the zebrafish mutant nrc have revealed that loss of SynJ1 also affects cone photoreceptor ribbon synapses, causing pronounced morphological and functional abnormalities. In this study we continue to examine the role of SynJ1 in photoreceptors. Using a newly generated antibody specific for zebrafish SynJ1, we localized this protein predominantly to cone photoreceptors. We then used blastula stage transplantation experiments to demonstrate that rods from nrc mutants lacking SynJ1 develop normally and do not have the pronounced morphological defects detected in cones. Given the known involvement of SynJ1 in synaptic vesicle endocytosis, we hypothesize that rods and cones use distinct mechanisms for vesicle recycling.Full Text Article
|Effect of chronic gestational treatment with the adenosine A1 receptor agonist R-phenylisopropyladenosine on metabotropic glutamate receptors/phospholipase C pathway in maternal and fetal brain.
David A León,José L Albasanz,Carlos A Castillo,Inmaculada Iglesias,Mairena Martín
Journal of neuroscience research 86 2008
Show Abstract
Pregnant Wistar rats were orally treated with the adenosine receptor agonist R-phenylisopropyladenosine (R-PIA) throughout the gestational period, and the status of the metabotropic glutamate (mGlu) receptor/phospholipase C transduction pathway from maternal and fetal brain was analyzed. In mothers' brains, radioligand binding assays revealed a significant decrease in the Bmax value, without any significant alteration in Kd value. Similar results were observed in the steady-state level of mGlu(1) and mGlu(5) receptors assayed by Western blot, suggesting that both receptor subtypes were modulated by chronic R-PIA treatment. mRNA coding mGlu(1) or mGlu(5) receptors was not altered, suggesting a posttranscriptional modulation as a possible mechanism of the loss of mGlu(1) and mGlu(5) receptors at the membrane surface. Moreover, phospholipase C stimulated by (R,S)-3,5-dihydroxyphenylglycine (DHPG), a selective agonist of group I mGlu receptors, was also significantly decreased after R-PIA treatment, suggesting a heterologous desensitization of group I mGlu/PLC pathway in maternal brain. Western blot and RT-PCR assays showed that neither alphaG(q/11) nor PLCbeta(1) was affected by R-PIA treatment. In fetal brain, no significant differences in mGlu receptors/PLC transduction pathway were observed after R-PIA treatment. These results suggest that chronic R-PIA intake during pregnancy modulates group I mGlu receptor signalling pathway in maternal brain, promoting a down-regulation of mGlu(1) and mGlu(5) receptors and a heterologous desensitization of the mGlu/PLC system.
|Effects of inflammation on the ultrastructural localization of spinal cord dorsal horn group I metabotropic glutamate receptors.
Mark H Pitcher, Alfredo Ribeiro-da-Silva, Terence J Coderre
The Journal of comparative neurology 505 412-23 2007
Show Abstract
Inflammatory pain is thought to induce functional plasticity of spinal dorsal horn neurons and may produce changes in glutamate receptor expression. Plasticity of group I metabotropic glutamate receptors (mGluR1 and mGluR5) is important in various neuronal systems, and these receptors are also known to modulate nociceptive neurotransmission in the spinal dorsal horn. The present study aimed at determining whether persistent inflammatory pain produces alterations in intracellular and plasma membrane-associated mGluR1alpha and mGluR5 in spinal cord dorsal horn. Persistent inflammation was induced in male Long Evans rats by a unilateral intraplantar injection of 100 muL of complete Freund's adjuvant (CFA). Three days after the CFA injection thermal withdrawal latencies were obtained prior to processing of transverse spinal cord sections for preembedding immunogold labeling after incubation in primary antibody for mGluR1alpha or mGluR5. Using electron microscopy, we quantified immunogold-labeled mGluR1alpha and mGluR5 profiles, located in lamina V and I-II, respectively, of both CFA-treated rats and untreated control rats. Compared to untreated rats, CFA-treated rats had a significant increase in the number of plasma membrane-associated mGluR5 immunogold-labeled particles in lamina I-II neurons of the spinal cord. Although no changes to mGluR1alpha expression were found in CFA-treated rats, plasma membrane-associated mGluR1alpha was significantly closer to the synapse. Therefore, in CFA-treated rats there was a specific increase in the ratio of plasma membrane-associated versus intracellular immunogold-labeled particles for mGluR5, and lateral movement of mGluR1alpha toward the synapse, indicating that peripheral inflammation-induced trafficking of group I mGluRs in spinal dorsal horn neurons may be an important factor in the development of plastic changes associated with inflammation-induced chronic pain.
|Molecular components and functions of the endocannabinoid system in mouse prefrontal cortex.
Lafourcade, M; Elezgarai, I; Mato, S; Bakiri, Y; Grandes, P; Manzoni, OJ
PloS one 2 e709 2007
Show Abstract
Cannabinoids have deleterious effects on prefrontal cortex (PFC)-mediated functions and multiple evidences link the endogenous cannabinoid (endocannabinoid) system, cannabis use and schizophrenia, a disease in which PFC functions are altered. Nonetheless, the molecular composition and the physiological functions of the endocannabinoid system in the PFC are unknown.Here, using electron microscopy we found that key proteins involved in endocannabinoid signaling are expressed in layers v/vi of the mouse prelimbic area of the PFC: presynaptic cannabinoid CB1 receptors (CB1R) faced postsynaptic mGluR5 while diacylglycerol lipase alpha (DGL-alpha), the enzyme generating the endocannabinoid 2-arachidonoyl-glycerol (2-AG) was expressed in the same dendritic processes as mGluR5. Activation of presynaptic CB1R strongly inhibited evoked excitatory post-synaptic currents. Prolonged synaptic stimulation at 10Hz induced a profound long-term depression (LTD) of layers V/VI excitatory inputs. The endocannabinoid -LTD was presynaptically expressed and depended on the activation of postsynaptic mGluR5, phospholipase C and a rise in postsynaptic Ca(2+) as predicted from the localization of the different components of the endocannabinoid system. Blocking the degradation of 2-AG (with URB 602) but not of anandamide (with URB 597) converted subthreshold tetanus to LTD-inducing ones. Moreover, inhibiting the synthesis of 2-AG with Tetrahydrolipstatin, blocked endocannabinoid-mediated LTD. All together, our data show that 2-AG mediates LTD at these synapses.Our data show that the endocannabinoid -retrograde signaling plays a prominent role in long-term synaptic plasticity at the excitatory synapses of the PFC. Alterations of endocannabinoid -mediated synaptic plasticity may participate to the etiology of PFC-related pathologies.Full Text Article
Data Sheet
|RABBIT ANTI- METABOTROPIC GLUTAMATE RECEPTOR 5 (mGluR5) AFFINITY PURIFIED POLYCLONAL ANTIBODY


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David Ortiz pins a lot of the Red Sox's 2012 woes on Bobby Valentine
Bobby Valentine was a dead man walking the day the Boston Red Sox announced he would be their next manager last winter.
Bobby Valentine was a dead man walking the day the Boston Red Sox announced he would be their next manager last winter.
Trevor Bauer has an incredibly unique throwing routine (see a video of it here) that worked out well for him during his days at UCLA and later in the minor leagues.
Mike Piazza has a new book called "Long Shot" that is being released this week, and it has received a good amount of press coverage thus far thanks to the New York media.
The Cleveland Indians have signed the remaining big free agent name left on the market, reportedly agreeing to a four-year, $48 million deal with centerfielder Michael Bourn.
Francisco Liriano was set to sign a two-year contract with the Pittsburgh Pirates this offseason, but the deal nearly fell apart when the left-hander suffered a broken arm.
The San Francisco Giants held their 2013 Fan Fest over the weekend, and as expected the World Series champions seemed to enjoy answering many of the questions they were asked.
San Francisco Giants first baseman Brandon Belt took a shot at the free-spending Los Angeles Dodgers while speaking at the team's "FanFest" event at AT&T Park Saturday.
Johan Santana threw the first no-hitter in New York Mets history last season, but he struggled mightily after that and finished the season 6-9 with a 4.85 ERA.

subset: commoncrawl


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Arts Feature
Free Art Fridays
Stephanie Galloway on the worldwide art movement right here in the Duke!
By Geoffrey Plant
What do you get when you cross online social networking with real people in real places making real art—for free?
Arts Feature
UNM's Three Millionth Book
First Edition of Sheriff Pat Garrett's book on Billy the Kid
By Kora Lee
On April 1, the Zimmerman Library at the University of New Mexico campus will hold a celebration officially welcoming the three millionth volume into their collection.
EVENT HORIZON (Saturday, Dec 3)
Reduce, Reuse and Recycle … Art
Recycle Santa Fe Art Festival
By Maggie Grimason
A showcase of art created from discarded materials.
EVENT HORIZON (Saturday, Dec 3)
Healing One Word at a Time
Women of the World Poetry Slam Championship
By Megan Reneau
Ten of the best female performance poets in Albuquerque compete to claim the title of Best Woman Slammer in Albuquerque.
EVENT HORIZON (Thursday, Dec 8)
Best in Snow
Jacob Marley's Christmas Carol
By Maggie Grimason
A retelling of the classic tale from the messenger's perspective.
EVENT HORIZON (Friday, Dec 9)
"The bigger the crime, the greater forgiveness."
Simon Gronowski
By Megan Reneau
Hear the harrowing expierence of the Belgian holocaust survivor and accomplished jazz pianist followed by a performance and a question and answer session.
EVENT HORIZON (Saturday, Dec 10)
Twirling Skirts of Tradition
Mariachi Christmas
By Nina Ferrell
Ring in the holiday season with exuberant music and dances from Mariachi Juvenil Aztlán and Ballet Folklórico University of Texas Rio Grande Valley.
Gil Dekel via Wikimedia / Commons Attribution-Share Alike 4.0 International
EVENT HORIZON (Saturday, Dec 10)
Chag Urim Sameach! • חג אורים שמח
Hanukat Shalom
By August March
A Hanukah party with food, silent auction, activities for kids, live klezmer music with dancing and a sing-along with The Neo-Weavers.
Courtesy of the JCC
EVENT HORIZON (Saturday, Dec 10)
OY! A Comedy with Chutzpah
By Megan Reneau
A collection of short comedies which are a comic tribute to all things Jewish and all things human.
EVENT HORIZON (Saturday, Dec 10)
No Good At Goodbyes
Kicking the Bucket List: 100 Downsizing and Organizing Things to Do Before You Die
By Joshua Lee
Death educator Gail Rubin discusses her newest book on organizing your life, death and legacy.
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Women of the World Poetry Slam Championship at Outpost Performance Space
Ten of the best female performance poets in Albuquerque compete to claim the title of Best Woman Slammer in Albuquerque.
Twentieth Annual Nutcracker on the Rocks at Keshet Center for the Arts
Con-Jikan at MCM Elegante HotelMore Recommended Events ››
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Lagging support among Hispanic voters for Democratic presidential candidate Hillary Clinton and congressional candidates in crucial races has stoked deep concern that the party and the presidential campaign are doing too little to galvanize a key constituency.
While Clinton holds a significant lead over Republican rival Donald Trump in every poll of Hispanic voters, less clear is whether these voters will turn out in numbers that Democrats are counting on to win. Clinton trails President Obamas 2012 performance in several Latino-rich states, including Florida, Nevada, Colorado and Arizona. In those same states, on which Democrats prospects of retaking the Senate hinge, some down-ballo


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Continental Breakfast
All overnight guests of the Castle, Highland Building, and Cottages can enjoy a complimentary light European Continental Breakfast, placed in your room before check-in, ready for enjoyment after you wake.
Suite Amenities
Every suite is equipped with a kitchenette that contains a mini-refrigerator for guests who bring their own snacks and food. The Castle has an ice machine and vending machines for guest use. Each suite has an ice bucket, along with cups, plates and silverware.
When making your Castle reservation, schedule an in-room therapeutic massage. Your retreat to the Castle can include a relaxing therapeutic massage from one of our Licensed Massage Therapists. Our Therapists can be scheduled for a thirty or sixty minute in-room therapeutic massage. Most Castle suites can accommodate two tables set up simultaneously so that you and your love can enjoy a therapeutic massage at the same time. Check with our staff about scheduling a therapeutic massage. You can prepay, including the service fee and gratuity. All you have to do is relax and feel the stress melt away.
Pool & Fitness Center
Opening Soon! Check back soon to see when the Pool & Fitness Center will reopen. After an extensive renovation, this Center will feature an indoor pool, hot tub, sauna, fitness center, lounge, and massage room. The south patio will be a perfect spot to enjoy the sun shining through the trees. Watch for details on how Castle guests can rent the Pool & Fitness Center after hours.


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This full service Salon has been providing premium service to it's clients at this location since 2001. They provide a very full line of salon services to meet their clients needs. The salon is very busy year round so keeping it's employees busy is not a problem.
Financials (USD)
Asking Price $199,500
Sales $412,987
Profit $88,366
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Disclaimer: MergerNetwork has not independently verified any of the information in this listing and makes no warranty as to its accuracy or completeness.


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6.5 miles away
American Express warmly welcomed
5' x 5' unit
5' x 10' unit
10' x 10' unit
9.5 miles away
2.5 miles away
The biggest factor when it comes to storage unit pricing in St. Augustine is size.
Below are some montly price ranges you can expect to see in St. Augustine based on different storage unit sizes.
The overall average price of a storage unit in St. Augustine is $26.
Of those who rented self-storage in St. Augustine, most booked a 5x10 storage unit.
View all the 5x10 units that are available in St. Augustine.
How does seasonality affect St. Augustine storage prices?
Storage prices in St. Augustine tend to be somewhat volatile when compared to other cities. Prices can vary by about 58.96 monthly depending on the time of year. St. Augustine storage prices are highest in February, and lowest in December.
Where Are the Best Hometown Fourth of July Celebrations?
You've heard of–and perhaps you've even seen–some of the flashiest Fourth of July celebrations in the U.S., in places like New York City, Philadelphia and Washington, DC. But...


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Reviews for Hana Japanese Steakhouse
Latest MenuPix Reviews
Gwen posted on 01/07/2013
Rating & Review:
We love this restaurant! Especially the kids. My daughter is a very picky eater and she eats everything on her plate before we leave. It's pricy but you always leave with to go boxes because of the amount of food you receive. Love this place!
Lana posted on 11/07/2010
Rating & Review:
I love this restaurant. I discovered it just while visiting the area. I was so pleased with the service and our chef was so funny and professional. I loved my experience so much I have been back and it is way out of my way. I recommend this restaurant to anyone who is in the mood for good food and entertainment.
5 year customer posted on 05/30/2009
Rating & Review:
KEEP DRIVING !! Hana's has gone to the birds. We've been eating at Hana's since 2005 but in the last six months the service and quality has become poor at best. The last two times we have eaten here the orders have been wrong and the food has been less than desirable. We waited for two hours on one of these occasions just to have the orders mixed up and cold. Staff is not capable of handling a crowd or managing the smallest of a crisis. I refuse to eat at or recommend this restaurant to anyone anymore and will suggest they drive to another town if sushi is a must.
lauren posted on 02/22/2009
Rating & Review:
this is a GREAT resturant i have only been a couple times and every time it was great. the food is great even the steak
Latest Yelp Reviews
Overall Rating:
Based on 54 Reviews
Based on 54 Reviews
Ted F. posted on 08/25/2016
Rating & Review:
We went to get something to go... Chicken teriyaki and beef teriyaki. It was very affordable The staff was very friendly and the wait short While... read more
Tulio M. posted on 12/04/2016
Rating & Review:
The sashimi deluxe was amazing! Although they could have included a bowl of rice. The chirashi is kinda the same thing, but with rice and way cheaper. They... read more
Rachel R. posted on 08/01/2015
Rating & Review:
I am in love with their sushi. It is a legitimate romance. While visiting my sister in Louisiana, she introduced us to her favorite sushi place. Being in... read more
Write a Review


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BROOKLYN - Brooklyn native and beloved hip-hop star Adam Yauch of the Beastie Boys died Friday after a three-year battle with cancer of the salivary gland.
Yauch,47, graduated from Edward R. Murrow High School in the early 1980s.
Yauch's death comes just a month after the Beastie Boys were inducted into the Rock and Roll Hall of Fame.


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Bracken Lodge Kennels
dog grooming, dog kennels, dog accommodation, clipping, de-matting, cutting, trimming, hand strippin
Bracken Lodge Grooming is your dog care in Ashford, Kent. Welcome to Bracken Lodge Kennels situated in Charing, Ashford. For over 25 years we have provided dedicated care and comfortable accommodation to dogs of all ages and breeds. We care about your dog as much as you do and pride ourselves on our excellent service and level of care, with plenty of attention and love dished out every day. Bracken Lodge Kennels also provides dog grooming. Our groomer is trained and experienced in all aspects of dog grooming including hand stripping, so let us brush up your pooch. Our welcoming and relaxing environment provides over 5 acres for dog exercise and play time. We welcome new and old visitors alike. For a happy stay, give us a call to arrange a viewing.
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Jillian Michaels Slim-Down: Weight Loss, Diet, Fitness, Workout & Exercise Solution
By Everyday Health, Inc.
Open iTunes to buy and download apps.
Looking for serious fitness and weight loss results? Try Jillian Michaels' no-nonsense, tough-love approach for a rock-hard body. Her official Jillian Michaels Slim-Down app will guide you day-by-day - motivating you until you achieve your own personal goal.
•Featured as one of Apple's Top Fitness Apps for 2013
You know Jillian Michaels as TV's Toughest Trainer. Now she's turned her winning approach into a personalized daily app to help the rest of the world get healthy, lose weight, and work their butt off! Reach your personal weight-loss and fitness goals with these tools:
•A killer workout and meal plan for total body results
•Fat-burning exercises to help you target problem areas
•Calorie Counter and Food and Fitness Journal to track your progress
•Barcode Scanner to automatically track nutrition facts from your packaged foods
•Daily tips from Jillian to motivate you!
•Weight Tracker to help you visualize and track success
•Walk & Run Tracker with motivational voice messages from JILLIAN! Her signature tough-love will keep you moving, as you track steps, distance, speed and calories burned. This app uses the GPS in your iPhone to track walks, runs, bike rides, and more!
•Weekly podcasts from "The Jillian Michaels Show."
Or become a subscriber and get even more:
•Personalized daily Jillian Michaels fitness plan with custom exercises and workouts, based on your body type and goals
•Customized meal plan based on your eating habits and goals.
•170 exercises ALL WITH easy-to-follow video instruction from Jillian.
•1,000+ delicious and metabolism-boosting recipes.
•Community support with thousands of active members through Jillian's message boards.
•Membership to JillianMichaels.com: sync your app completely with the website and get unlimited access to 100s more recipes, diet tips, workouts, fitness-advice and exercise videos, custom meal plans, food and fitness tracking journal, dining out guide, calorie counter, interactive weight tracker, weight-loss buddies, wellness tools and so much more!
•No Ads!
•Your choice of billing plans: $3.99/ week, $14.99/ month or just $79.99/ year. Just use your iTunes login to subscribe and pay!
•Payment will be charged to iTunes Account at confirmation of purchase
•Subscription automatically renews unless auto-renew is turned off at least 24-hours before the end of the current period
•Account will be charged $3.99/ week, $14.99/ month or just $79.99/ year for renewal within 24-hours prior to the end of the current period
•Subscriptions and auto-renewal can be managed by the user under Account Settings after purchase
•No cancellation of the current subscription is allowed during active subscription period
Discover In-App Purchases to Target Your Personal Goals:
•Not ready to subscribe? Buy exercise packs to target and transform your back, butt, lower body, torso/ core or your total body with Jillian's expert coaching and moves!
Continued use of GPS running in the background can dramatically decrease battery life.
What's New in Version 6.1.1
• Bug fixes and stability improvements.
Customer Reviews
Recipes in app purchase is a joke
So we paid to upgrade, the over 1500 is a joke. Some are good most are( and I'm not joking) junk. Example: there is a recipe for cereal and milk. States Pour cereal into a bowl. Top with milk and serve. Seriously. Another states, Rinse apple in cold water and pat dry. There are 3 of them all for apples but for different sizes. One more just for kicks. Baby Carrots: place carrots in small bowl or on a plate and serve. So 1500 is an overstatement. Now it does state the nutritional facts for everything but that is already free from the app. We were looking for great recipes and learned how to put peanut butter on celery. So they got my money for a week, please save your money.
Could be better!
The app doesn't work well, especially when it comes to tracking your steps. I'm a nurse and I'm running around on my feet all night but it doesn't track your steps unless its monitoring in a work out, trust me I get a good work out taking care of people! But the steps don't count because I'm unable to keep the app running in order to save my battery.
It it worked I would like it...
Just bought Jillians program and was trying to use the app. It doesn't save my daily weight or calorie intake suggestion. It also won't allow me to see my planner only if I go online to the actual website do I actually see the workouts I need do do for that day. Spent too much money on something that doesn't work. Has potential but needs to fix the bugs.
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- Free
- Category: Health & Fitness
- Updated: Apr 26, 2016
- Version: 6.1.1
- Size: 101 MB
- Language: English
- Seller: Everyday Health, Inc.
- Copyright © 2014 Everyday Health, Inc.
Compatibility: Requires iOS 7.0 or later. Compatible with iPhone, iPad, and iPod touch.
Top In-App Purchases
- Jillian Michaels Fitness Plan$3.99
- Jillian Michaels Fitness Plan$14.99
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The Experience
If you're searching for the perfect combination of beach fun and natural discovery, look no further. Home to ancient Mayans and brimming full of historical and archaeological wonders, the Riviera Maya will have you gawping in awe.
Located just minutes away from Cancun and Playa del Carmen, this all-inclusive five-star resort offers unparalleled luxury and faces a glorious stretch of white sand and turquoise waters. Those with a taste for adventure can snorkel through ancient caves and underwater gardens, explore the remains of an ancient Mayan village and swim with dolphins at the eco-archaeological Xcaret Park next to the resort.
- Limitless all-inclusive fun and thrills for everyone in a beautiful eco-reserve facing a glorious beach
- Enjoy access to 11 Restaurants and 10 Bars & access to 5 Swimming Pools
- Exclusive discount to the eco-archaeological Xcaret Park
Included flights
UK Departure
- Departs: london
- Arrives: cancun, Mexico
Return Flight
- Departs: cancun, Mexico
- Arrives: london
Occidental at Xcaret Destination
- Deluxe
- All Inclusive
- Exceptional all-inclusive offer that includes unlimited meals, snacks and beverages
- Spectacular location beside a pristine beach and Xcaret Park
- Excellent kids club with endless activities and babysitting services
Occidental at Xcaret Destination enjoys a wonderful location within an ecological and historical reserve, beside a private powder-soft Caribbean beach. The resort's unique setting features meandering river channels and tropical vegetation, an ideal eco-holiday destination. The resort offers an array of leisure facilities including a large free form pool and spa. There is a wide selection of restaurants and bars and the excitement and attractions of Playa del Carmen are just 5 minutes away. Whether you're looking for a quiet romantic escape or a fun family atmosphere, everything you could want from a holiday in the Riviera Maya is on offer at Occidental at Xcaret Destination.
Occidental at Xcaret Destination accredited by the Rainforest Alliance!
The hotel has been distinguished in the Riviera Maya as a hotel that includes into our daily activities ecoâfriendly practices as a gesture of interest in the impact on our planet, plants, animals and community.
âââââWhy Not Upgrade to the Unlimited Xcaret Xperience and enjoy the following added values:
At Occidental at Xcaret Destination:
- Exclusive All Inclusive wristband giving guests all day unlimited multi-entrance access to Xcaret Park
- VIP check in with Mayan welcome
- In-room robes and slippers during the whole stay
- Daily complimentary stocked minibar
- Unlimited reservations at speciality restaurants during the whole stay (subject to availability)
- 11 International restaurants and 10 bars
- 5 pools, day & night time activities and entertainment program, Kids Club
- 30% Off a 90 minute "Wayaka" Mayan Massage
- $920 USD Resort Credit*
At Xcaret Park:
- All day unlimited mutli-entrance access to Xcaret Parks
- Complimentary access to the kids activity "Xiquitos Sonando (Dreaming Kids) - children participate in a guided visit to Xcaret
- Reserved area seating at the Gran Tlachco theater during the main presentation
- 10% Off meals & beverages at Xcaret park
$920 USD in resort credit includes:
$500 RESORT CREDIT AT Occidental at Xcaret Destination
- (2) $70 vouchers applicable toward a Royal Club room upgrade*
- (2) $50 vouchers applicable toward green fees at Playacar Golf Club or TPC Cancun Golf Club
- (4) $35 vouchers for Spa treatments (products not included)
- (2) $25 vouchers for use at select Pueblo del Rio shops
- (2) $20 vouchers applicable toward a Romantic Dinner
- (3) $10 vouchers applicable toward our wine list (1 voucher per bottle) *subject to availability
- (2) $10 vouchers for the Sea Trek Tour
- (2) $10 vouchers for the Shark's Interactive Adventure
- (2) $10 vouchers for Rays Fish Interactive Encounter
- (2) $10 vouchers for the Sea Trek Ray Fish Encounter
- (2) $10 vouchers for the Tlacho Dinner "Xcaret Mexico Espectacular" show
- (2) $10 vouchers for the Temazcal purifying experience
- (2) $10 vouchers for the Snorkel Tour
- (2) $10 vouchers for the Scuba Tour around Xcaret Bay
- (2) $10 vouchers for Wine Tasting $140 credit in Tour Tickets
- (2) $35 vouchers for the Xichen Classic Tour
- (2) $35 vouchers for Ex-Balam Rio Lagartos Tour $100 credit at Xel-ha Park
- (2) $10 vouchers for Dolphin Trek
- (2) $10 vouchers for Sea Trek
- (2) $10 vouchers for Scuba Caleta or Scuba Cenote
- (2) $10 vouchers for the Manatee Encounter
- (2) $10 vouchers for the Ray Fish Encounter
this is my 3rd time at the hotel and I always pay a little extra to have unlimited access to xcaret park beside the hotel as there are so many things to do there and the shows are terrific.it's only a short walk to the restaurants ,beach and park from building 14.Everything including specialty...
Sarah Hosford reviewing Occidental at Xcaret Destination (07-Dec-2016)
£989 per person
Your holidays include:
- Flight
- Accommodation
- Representation Services


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Gairloch Highland Lodge
Wester Ross, Ross Shire, Ullapool IV21 2AH, United Kingdom
Find Hotel Rates
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Gairloch Highland Lodge Hotel Reviews
Based on reviews from across the web.
Gairloch Highland Lodge Hotel Details
Check-in: 14:30 .. 20:30
Check-out: 07:00 .. 10:30
Affording a friendly and informal atmosphere, the hotel comprises 2 floors with a total of 17 rooms. As well as an inviting lobby with a hotel safe, the hotel also offers its guests a cosy bar, a pleasant caf and an appealing restaurant (with highchairs for children). Business guests are offered use of a conference room, the public Internet terminal as well as room and laundry services. There is also a car park for all those arriving by car. The hotel has one wheelchair-accessible room.
Wester Ross, Ross Shire Ullapool IV21 2AH, United Kingdom


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One Ball Jay Beardo Traction Pad
Description: One Ball Jay Beardo Traction Pad
If you can grow a mighty beard, congratulations, if not, no worries because One Ball Jay can at least give your board a beard with the Beardo Traction Pad for stompage fun. There's a one letter difference between board and beard, there isn't much significance for that but we thought you should be aware of their spelling kinship.
Features of the One Ball Jay Beardo Traction Pad
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Lic. # 806577
P.O. Box 929, Nipomo, CA 93444
Nipomo, CA
Mailing Adress:
P.O. Box 929
Nipomo, CA 93444
(805) 929-2695
(805) 929-2696
Contact US
Silver Oak Construction & Concrete, Inc. is a concrete contractor serving the San Luis Obispo and Santa Barbara Counties. We pride ourselves in our high customer satisfation rate and our attention to detail that has placed us among the top concrete contractors in the central coast. Our project resume ranges from large scale Commercial Buildings to Wineries to Private Residential Concrete Work. Whether your project is big or small, give us a call with any questions or if you would like us to provide you a quote.
Project Photos


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Irrfan, Deepika will play Sahir and Amrita role661 views
Abdul Hayee knew Sahir Ludhianvi, who is a legendary poet of India, is going to be filmed as a romantic poet of the subcontinent. He was also a film lyricist who wrote in the Hindi and Urdu languages. His work influenced Indian cinema, in particular, Bollywood film. Sahir Ludhianvi and Amrita Pritam will come to live in Sanjay Leela Bhansali's next movie, Gustakhiyan. The biopic chronicling the stormy relationship between poet Sahir Ladhianvi and author Amrita Pritam.
Whereas it is confirmed that Indian veteran actor Irrfan will play the role of Ludhianvi but there are many speculations regarding that who will play Amrita Pritam's role.
Previously, it was recommended that Bajirao Mastani leading actress Priyanka Chopra may perform the poet, but recently there is a rumor that the director has signed his other Bajirao Mastani leading actress, Deepika Padukone against Irrfan.
It was reported as "Since Priyanka got along like a house on fire with the Gustakhiyan producer Sanjay Bhansali, the reports of her stepping into author Amrita Pritam's role were taken to be authentic. However the truth is Priyanka is not doing Gustakhiyan."
Both actors Deepika and Irrfan have appeared earlier as a pair in Piku with Big B and Gustakhiyan would be their second pairing. In Piku they have played a tentative pair to great triumph. In the upcoming movie, nevertheless, they will have a full-fledged romance, so it's better to know the two performers have bankable chemistry.
Deepika tolerates a weird similarity to Amrita Pritam, whereas Irrfan Khan absolutely has the acting chops to pull off the Sahir Ludhianvi's character
On the other hand the veteran actors, Priyanka, and Irrfan, have done a film, Saat Khoon Maaf jointly, which was not quite the box office hit.


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Category:Year of establishment unknown
From Wikipedia, the free encyclopedia
|This category is not shown on its member pages unless the appropriate user preference is set.
This category is for articles about companies, groups and organizations where the articles do not have a year of establishment because the year is not known and is never likely to be known (e.g. historical organizations). If the year is likely to be known, and could be researched (e.g. contemporary or recent figures), please use Category:Year of establishment missing instead.
Pages in category "Year of establishment unknown"
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This is the one you have been waiting for! A great ranch in a great location with all the comfort you have been looking for. Floor plan flows from kitchen to dining area to spacious living room. 3 good size bedrooms and lots of storage. Main bath is handicap friendly plus additional 1/2 bath in the lower level. Lower level could be finished for a family room. Private deck off the dining area gives a pleasant view of the yard and YES, there is a 2 Car Garage! All this at a very affordable price! Call now
Features Single Family
|Garage: 2 car, Attached, Extra Storage Area, Heated, Opener inc., Tandem
|Estimated Age: 1957
|Finished SqFt: 1,176
|Finished Above Grade SqFt: 1,176
|Style: Ranch
|Elementary School: Lincoln
|Middle School: Beaver Dam
|High School: Beaver Dam
|School District: Beaver Dam
|Taxes: $2,890
|Body of Water:
|Room Type
|Room Size
|Master Bedroom
|2nd Bedroom
|3rd Bedroom
|Living/Great Rm
|Laundry Room
Estimate your monthly mortgage payment
How much can I borrow?
Apply online: www.shelter-mortgage.com
Speak with a mortgage consultant: 800-981-8139


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Description: From Dritz, this package contains 3 yards of 1/2'' knit elastic. Perfect for waistbands, sleeves, necklines and legbands. Insert into casing or sew directly onto wrong side of fabric.
go to product
Description: From Dritz, this knitted elastic is soft and colorful! It can be sewn easily to make waistbands, cuffs and gathers. Great colors give you the option to let this elastic show! Create an easy case-free waistband for your garment or ...
Description: This soft and delicate elastic fold over fabric edges and seams to reveal a scalloped picot lace detail. Can be used as a functional stretch elastic with a decorative edge or simply stitched to a fabric edge for a dainty ...
Explore Fabric.com
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Sewing Center


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Home | Make an Appointment | For Medical Professionals
During this test, a safe gas is given through a mask or a medication is given through a catheter in your vein for a short period of time. Pressure measurements are taken before and after by your heart provider. The results will be used to decide which medication is best to treat the elevated pressure in your heart and lungs.
Learn how your generositycreates the future of medicine.
© 2012-2015 Mayo Clinic Health System Notice of Nondiscrimination Notice of Privacy Practices Terms of use


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Seagate 50pk 500gb Momentus Thin SATA 5400 RPM 16mb 2.5in / Mfr. No.: St500lt015-50pk
2.5 SATA Hard Drive with Laptop Encryption
Thin and light laptop hard drives with price and capacity points that help to enable no-compromise, lightweight designs for laptop computing.
- 7mm Laptop Thin HDDs or 9.5mm Laptop hard drive kits
- Seagate SmartAlign™ technology1 provides easy transition to 4K sectors
- Available as a Self-Encrypting Drive (SED) and FIPS 140-2 SED2
- Compatible with SATA 6Gb/s designs
- Backed by a 2-year limited warranty
2.5-inch Laptop Drives Available in Several Capacities
Seagate's purpose-built the 2.5-inch Laptop HDDs help meet our customers' demand for affordable, flexible computing solutions.
- 7mm hard drive is available in 500GB, 320GB, 250GB and 160GB capacity options
- 9.5mm hard drive is available in 2TB, 1TB and 500GB capacity options (Kits)
Smooth Migration to Advanced Format 4K
Laptop Thin HDDs include Seagate SmartAlign technology.1 This innovation helps you easily get all the benefits of the new Advanced Format (AF) 4K sector standard—no utilities, no extra steps—automatically in the background. Performance is not affected, and there is no need for time-consuming utilities required by other AF-enabled drives.
Secure Laptop Encryption (SED) Options
Laptop Thin SEDs deliver government-grade encryption without performance degradation—protecting your data where it lives. You get all the benefits of secure data encryption without the headaches.
- Ships with the Trusted Computing Group (TCG) Opal protocol
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- Instant Secure EraseTM technology that can instantly erase the security key data on your hard drive if needed, enabling secure disposal or repurposing of the SED
SATA 6Gb/s
Spin Speed
Operating Temperature
Average Operating Power


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Actual rating will vary with options, driving conditions, habits and vehicle condition.
We are excited to offer this 2014 Ford F-150.
Rest assured with your purchase of this pre-owned Ford F-150 XLT. Because a CARFAX BuyBack Guarantee is included, you have built-in peace of mind to drive off the lot confidently. It's not often you find just the vehicle you are looking for AND with low mileage. This is your chance to take home a gently used and barely driven Ford F-150.
There are many vehicles on the market but if you are looking for a vehicle that will perform as good as it looks then this Ford F-150 XLT is the one!
More information about the 2014 Ford F-150:
With three cab styles, three different bed lengths, and a wide range of models and optional equipment, there's an F-150 configuration for every truck need. Ford also boasts that its F-150 has the only full-size pickup with 6-speed automatic transmissions across the entire lineup. Another feature that stands out, even against rival models from Ford, Chevy and GMC is MyFord Touch, an all-encompassing connectivity and infotainment system.
Interesting features of this model are standout refinement and ride comfort, A model and configuration for every truck need, fuel-efficient powertrain lineup, and strong towing and payload ratings
We look forward to seeing you soon! Please call us for more information.
Estimated Monthly Payment will depend on vehicle make and model and will be determined upon actual vehicle inspection. All payments are estimates. Tax, title and any other fees are extra.


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Otolaryngology - 110908
Gastroenterology (w/Procedures) - 117424
Garden Ob/Gyn is a group with over 8 locations throughout the New York City and Long Island area. Our organization places a high value on quality, caring and compassionate staff.
Family Medicine/ $225k Salary/Oupatient
Outpatient Opportunities in NY eligible for Loan Repayment!
Psychiatry - New York City - Provide Care for the Homeless Patient Population
Dermatologist needed for busy high-profile dermatology and laser practice in New York City!
The Department of Pediatrics at Northwell Health is seeking BC/BE Pediatric Hospitalists to join our team at Lenox Hill Hospital.
Interventional cardiologist Brooklyn/New York metro area
$600,000 income
Pediatric Emergency Medicine Physician
Internal Medicine - 116749
Family Medicine - 110388
Psychiatry - 111164
Internal Medicine - 106906
OBGYN with interest in family planning (no OB, no night calls). Work schedule Tuesday-Saturday.
Orthopedic Surgeon, $550,000+ Base Salary, Partnership Track
Academic Stroke Position in Downstate New York
Board Certified OMFS seeking a committed individual to become partner in Eastern Long Island practice, well established within the community.
Fulltime Intensivist Telemedicine job available in New York City, New York.
Fantastic Urgent Care physician job opportunity in northern New Jersey!
Thousands of new jobs posted every day. Let us bring them to you. Sign up for alerts.


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Glam was founded in 2004 with the vision to create engaging experiences for consumers by packaging brand advertising with professional digital content.
Formed from merger of Datran Media and CONTEXTWEB, providing contextual advertising, ad exchange, and email marketing solutions.
PulsePoint is leading in Top 10K Sites, Top 100K Sites, Top 1M Sites and The Entire Web.
In terms of market share, Glam Media is clearly lagging behind, losing to PulsePoint in all segments.
Glam Media has better usage coverage in more websites categories. Including People & Society, Arts & Entertainment, Internet & Telecom, Business & Industry and 99 other categories.
PulsePoint is leading in News & Media, Food & Drink, Cooking & Recipes, Computer & Electronics and 92 other categories.
PulsePoint is leading in most countries, including United States, France, Japan, Egypt and 83 other countries.
Glam Media is leading in United Kingdom, Canada, Germany, Brazil and 58 other countries.


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The Meaning of ALRYT
You have found the definitive source for the meaning of ALRYT. This page contains all the definitions of ALRYT, crowd sourced, moderated and curated by real people who care about the true definitions of ALRYT.
The Top Definition of ALRYT
The most popular meaning of ALRYT is: alright
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ALRYT is alright
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The definition of ALRYT is "alright".
ALRYT Definition
The meaning of ALRYT
ALRYT means alright.
Now you understand the definition of ALRYT - ALRYT means "alright".
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What does ALRYT mean? ALRYT is an acronym, abbreviation or slang word that is explained above. If you ever forget what ALRYT means, just come back to Slang.org and we'll define any acronym you need help with.


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Force 4
Halyard Bag Large Single Blue
- Details
- Reviews
halyard bag Large Single Blue - Keep your cockpit or coach roof free of untidy rope tails with these quality British made canvas Halyard/Sheet Bags. With press stud poppers they can be mounted in the cockpit for sheets and halyards, and I use one at the end of the boom to tidy up those endless slab-reefing lines. Available in Blue and in 4 sizes. 12"x20"


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Ask About This Home
This solidly built and well maintained home is ready to move in. Located on the 17th fairway of Kingwood Golf course. Open floor plan with lots of windows to enjoy view of golf course and mountains. Master and laundry on main level. Hardwood floors and stone fireplace. Decks on upper and lower level. Easy access.


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Utilizing the arrow keys on your keyboard you can rapidly move the cones to the left and right as they make their method across the hectic road. The fate of the similarity Mr Potato Head, Slinky Pet dog and Rex are literally in your hands as big lorries, racing fire truck and vehicles hurtle towards you.
As the Cone Chaos video game gets progressively harder, the traffic accelerate. If it gets too tense, you can constantly hide at a manhole. Overall this is a quick-paced video game which is simple to discover and entertaining to play.
In the second video game in this title, Toy Shelf Showdown, you help Buzz take on and defeat the wicked Emperor Zurg. The job is to assemble a space rocket while preventing hostile robotics and great deals of bouncing balls. This is an action-packed video game with great deals of various twists and turns. Buzz can stun the robotics with lasers and if you run out of shots, you need to discover a battery for recharging.
Both animated video games are presented by Buzz and Woody and there ready clear instructions with the title on how to play and where to get help. This is your opportunity, as Buzz states, to go to "interactivity and beyond."--Justin Hunt


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LEWISVILLE (CBSDFW.COM) – Police in Lewisville are asking for help identifying a man who robbed a pharmacy earlier this week.
The suspect pulled a handgun on a worker at the CVS Pharmacy, located in the 100 block of West Corporate Drive.
Police say a man went to the pharmacy, pulled a gun out of his pocket, and told the pharmacist to get him several specific drugs. The robber gave the pharmacist a time limit to comply, which he did.
Witnesses told police they saw the suspect leave from the pharmacy in a dark-colored (possibly green, black, or blue) 4-door passenger car.
The suspect is described as a dark-skinned white man, standing about 5'10" and weighing 170 pounds. He had longer black hair and at the time of the crime was wearing a tan or gold-colored shirt and blue jeans.
Anyone with information about the robbery or the wanted suspect is asked to call the Lewisville Police Department's Tip Line at 972-219-TIPS (8477) or call Denton County Crime Stoppers at 800-388-TIPS (8477).
Latest News:
- Major Survey Of Transgender Americans Finds Widespread Bias
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- DFW Salvation Army In Desperate Need Of Bell Ringers
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Sci fi/Fantasy image bySerrania Drake
A Chain is only as strong as its weakest link... Here we have Kyuubi no Kitsune, or "究極 (kyuukyoku- Ultimate)" if it must be named, talking to Tsuki who is a tiny tiny dot that is really hard to see. But she is there. Nine-tales is not talking to itself. It's not that mad.
Published More than a year ago
Category Anime/Manga
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Directed by New York Times Crossword Puzzle Editor Will Shortz, this is the US's oldest and largest crossword competition. Solvers tackle eight original crosswords created and edited specially for this event. Scoring is based on accuracy and speed. Prizes are awarded in more than 20 categories, including a $5,000 grand prize. Evening games, guest speakers, and a wine and cheese reception allow solvers to meet each other in a relaxed and entertaining atmosphere.
This site contains handmade Logic and Sudoku puzzles by the best puzzle creators for the puzzle lovers of all age groups. All the puzzles in this website are free of cost.
- Home
- Fun Brain Teasers
- Sudoku
- Sudoku Variations
- Online Puzzles
- Puzzles
- Games
- Contact Me
- Subscribe
- Download
Friday, February 17, 2012
35th American Crossword Puzzle Tournament
Posted by Rajesh Kumar at 7:36:00 PM
Labels: Puzzle Championships
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25 Years of Microsoft Office Roadkill
Love it or hate it, Microsoft Office has torn up the competition, leaving all manner of software carrion in its wake.
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Product Details
- beautifully hand made product
- aluminium
- wood
- nordic
Product description
Please note these lamps require a Denmark to UK plug adaptor (not supplied).
This Scandinavian lamp features a natural wood base and adjustable 'arm' combined with contrasting aluminium shade and versatile fixings. A good choice for a contemporary interior.
Dimensions: H72 x W22cm
Questions about the Anglepoise Lamp With Aluminium Shade
Do you have a question about this product?
If your question isn't answered below, drop us an email using the form on the left. We'll respond to you personally by email and may also post the answer here to help others.
We're here to help! Fire away.


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By Elizabeth Pantley, Author of Kid Cooperation and Perfect Parenting
We're expecting our first baby soon and thinking about using a family bed. We've done a lot of research on the "whys" ¾ and there's lots of information out there. But what about the practical tips? How do we set things up?
Learn about it
The family bed, co-sleeping, shared sleep ¾ no matter what you call it, it means that your baby sleeps with you, or very close to you. The family bed is becoming more and more common (or perhaps it's always been common but more people are now talking about it.) Sharing sleep is very popular with parents (particularly nursing mothers) of young babies who wake throughout the night, since it allows parents to avoid getting up out of bed and traveling up and down a dark hallway. Co-sleeping is popular also with parents of older babies who enjoy the nighttime closeness with their child.
There are as many different styles of family beds as there are families! Here are a few of the typical sleeping arrangements:
The family bed: Parents and baby sleep together in one bed ¾ usually king-sized.
Side-by-side: The child sleeps on aseparate mattress or futon on the floor next to the parent's bed.
Sidecar: A cradle or crib is nestled adjacent to the parent's bed, sometimes with one side of the crib removed.
Shared room: The baby and parents have separate beds in the same room.
The use of these arrangements varies from home to home also. Some of the common sleep situations are:
Shared sleep with the baby during the night and for naps.
Part-time shared sleep for either naps or nighttime only, or some of both, with baby in a crib, cradle or other place for other sleep times.
Mom's dual beds is a common setup in which Mommy has one place where she sleeps with the baby, and another where she sleeps with her husband. She moves back and forth between beds based on how often the baby wakes up and how tired she ison any given night.
Musical beds are a common arrangement. There are several beds in different rooms, and parents and baby shift from place to place depending on each evening's situation.
Occasional family bed is when the baby has her own crib or bed but is welcomed into the parent's bed whenever she has a bad dream, feels sick, or needs some extra cuddle time.
Sibling bed is often a natural followup to the family bed. Older children share sleep after they outgrow the need for the parent's bed or the sidecar arrangement.


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I have never had a Bad experience With Jacks Small Engines. They are very helpful on the phone Etc.. I have been dealing with them for almost 15 years. THANKS JACKS SMALL ENGINES.
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Much better than I was expecting for the price. Great shipping time. make cut look great.
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Really fast shipping.These blades really cut well.The discharge is much smaller than with standard blades.
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Secrets of the Sky: What to Know Before Your Next Flight
Pilots across the country reveal everything from safest seats to dirtiest areas on planes.
Plane Etiquette When Someone Falls Asleep on Your Lap
David Wright with solutions to dealing with awkward travel issues when flying.
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(817) 542-0890(817) 542-0890
1825 West Division Street, Arlington, TX 76012
With so many disreputable flooring companies out there, you need a professional you can explicitly trust. You need our over-35 years of experience for the highest quality flooring and carpeting for your home or business.
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"" Nevada Favorite Tip by Krystynn
Nevada General: 75 reviews and 57 photos
Fondest memory: This is the spectacular Elephant Rock found here in the Valley of Fire. Isn't it awesome?
There are a few viewpoints which you must not miss seeing and these notable places include:

 Petrified Logs: Several colorful fossilized tree trunks, safely fenced off to deter souvenir hunters. Lovely! I mean... the tree trunks.

 The Beehives: Weathered, layered sandstone mounds (originally sand dunes in an ancient desert), now preserved in stone!

 Mouse's Tank: A natural cavity in the rocks where rainwater collects.... and used as a water source by a Paiute Indian fugitive in the 1890s. This was not of lasting value to him as he was captured and eventually killed after several months of hiding. Anyway, the trail that leads to the Tank passes through various petroglyphs dating from as far back as 1200s.

 The White Domes: A separate region with a varied desert vegetation and more rocks of contrasting color (!!).... reached by a 14KM scenic drive/ hiking trail.

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Mitsubishi Electric 260L Classic Style Top Mount FridgeMR260GWA 9316524164236
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Designed for a classic look, the Mitsubishi Electric 260L Classic Style Top Mount Fridge is a functional addition to your kitchen. Tempered glass shelves are built to hold up to 101kg in weight, and an adjustable door shelf offers storage that you can modify for your individual needs.
Key Features
- The energy efficient LED light brings brightness to your Mitsubishi Electric fridge 50 times longer than the average bulb.
- An anti-bacterial food liner, combined with a titanium filter, ensures a hygienic environment for your food.
- A sliding chilled case is a great solution for easy access to your dairy products, fresh fish or meat, and chilled drinks.
- The classic style Mitsubishi Electric fridge includes a revolving ice tray that releases cubes with a simple twist of the handle.
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|2 Stars
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If you feel your product has failed a Consumer Guarantee (as defined by Australian Consumer Law) then don't worry you can easily return it to your local Harvey Norman store. We'll even pay for the postage if you live in an area where there is no store. Our returns policy covers any purchased item (conditions apply).
Protect your purchase with Product Care®. Get a brand new replacement if your product suffers an eligible fault through our hassle free support process, plus enjoy a range of exclusive benefits like a product health check and free prints each year so you experience your new product even more.
Discover all of the benefits Product Care® brings you:
With Product Care® you can relax knowing your purchase is protected. After an assessment, if your product suffers a fault covered by the terms of your plan, we'll arrange to have a brand new replacement delivered to your doorstep.
Our range of support options ensure you experience an easy and hassle free process when you need to talk to us about your Product Care® plan. Whether you are experiencing a fault, or wanting to redeem your entitlements, we will provide you with guidance and support every step of the way.
Having Product Care® with your purchase means you can enjoy a range of special benefits that are exclusive to you and will help you enjoy the experience of your new product even more.
Conditions apply. This section should always be read in conjunction with the Terms and Conditions for Product Care® Replacement (Electrical).
Learn more about Product Care®.
Either complete the details below or chat to a staff member online.
Interest Free
- 50 Months Instalment Interest Free - Equal With Monthly Payments. Offer ends 24/12/2016.
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October Gonzalez sits down with Channel 2's Erin Coleman
Updated:October Gonzalez, wife of Atlanta Falcon's tight end Tony Gonzalez talks about his retirment, their children and being part of the Falcons family.
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'Tis the season to eat, drink, and be merry, and while we can't help you dress your tree or turkey, we can offer our style guidance to help you outfit the perfect party ensemble - whether you're headed to an office event, a cocktail affair, or a postwork soiree.
|4 Foolproof Ways to Dress Up For Any Holiday Party
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About the Fax Service Client API
The fax service provides fax services to clients on a local area network. The Fax Service Client API allows developers to incorporate these services and enable basic fax functionality in their applications.
The Fax Service Client API includes both Microsoft Win32 functions and Component Object Model (COM) interfaces.
In the Win32 Environment
The Win32 functions are implemented as a DLL. This is a client/server implementation that permits a fax client to communicate with the fax server using remote procedure call (RPC). The client computer can use a shared fax printer on the network to access outbound fax services, or the application can call functions in the Fax Service Client API to connect to the local fax server to transmit a fax.
In the COM Implementation Environment
The COM interfaces are implemented as dual interfaces to accommodate both Microsoft Visual Basic programmers and C/C++ programmers. This document provides reference material for both programming languages. For more information, see Using the Fax Client COM Implementation.
The following diagram illustrates an overview of the fax client application environment.
You must include the WinFax.h header file in your source code files. To use the fax client COM implementation that Microsoft supplies, you must include FaxCom.h.
Build date: 5/5/2012


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Magna International Inc.
- Jaguar Land Rover wants to make electric cars in Brexit U.K.The Japan TimesCoventry
- Eight major market moves after Trump's shocking winThe Globe and Mail
- Magna backs out of plan to relocate its head officeThe Globe and Mail
- Decostar increases footprint to meet demandbizjournals.comSouth Carolina
- Royal Group's De Zen donates $10-million for new Vaughan hospitalThe Globe and MailLas Vegas
- Magna International Inc to buy BOCO Group to gain two factories that make automotive latches, hingesbusiness.financialpost.com
- Magna to acquire BOCO Group, two factories that make automotive latches, hingesThe Globe and Mail
- WMagna considering new car plants after getting BMW 5-series dealwhitecourtpress.com
- Magna International Inc wins contract to build BMW's new 5 Series sedanbusiness.financialpost.comGermany
- Magna mulls new car plants after securing BMW 5-series deal at flagship Austrian plantbusiness.financialpost.comGermany
- Magna to make BMW's 5 Series sedans at Austrian plantThe Globe and MailGermany
- Three automotive stocks that deserve a closer look under the hoodThe Globe and Mail
- TOnex co-founder guilty in gross negligence tax casetorontosun.com
- Magna shares slump as U.K. expansion comes amid Brexit falloutThe Globe and MailChina
- Seating supplier moving from Strongsville to Sheffield, closer to Ford plantchronicle.northcoastnow.comAsia
- Magna International Inc says it's ready to manufacture vehicles for new entrants as Apple rumours swirlbusiness.financialpost.com
- Auto parts firm Magna International signs deal for German company Telemotive AGbusiness.financialpost.com
- Will Canada's Magna build long-rumoured Apple car?thestar.com
- Magna executive pay packets slip despite annual profit gainThe Globe and Mail
- The success stories of dual-class shares miss an incontrovertible truthThe Globe and Mail
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Newstral's extraction of names of people and organisations from the world's news is completely automated. If you do not want your name to appear here, please contact us. We will be happy to look at your request.