text:
The ninth book of the New York Times bestselling series and basis for LONGMIRE, the hit drama series now on Netflix
Craig Johnson's The Highwayman is now available from Viking. An Obvious Fact will be available in September 2016.
It's homecoming for the Durant Dogies when Cord Lynear, a Mormon "lost boy" forced off his compound for rebellious behavior, shows up in Absaroka County. Without much guidance, divine or otherwise, Sheriff Walt Longmire, Victoria… (more)
While the genre of western fiction has never really died, its resurgence, due to this series and others, is most welcome. Read A Serpent's Tooth and find out why.
The mystery here is complex and satisfying, tense and dark, but it is, as always, Walt who carries the book on his very capable—if sarcastic—shoulders.
Publisher: Penguin (June 04, 2013)
Format: EPUB
Page count: 352 pages
File size: 6.8 MB
Protection: DRM
Language: English


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Femella Blue Printed Kimono Cape With Fringes
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- Make a lasting impression with this printed, blue colored cape. Made from 100% polyester, this printed cape has comfort fit. It can be teamed with jeans or shorts to sport a fresh and trendy look.
- Fit - Regular fit
- Color - Blue
- Model fit - Model is 5'7"/170cms and is wearing medium
- Wash Care - Cold Wash Only
- Fabric - 100% Polyester
- Product Listing Id :MP000000000066813
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subset: commoncrawl


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Ted Cruz, a prominent U. S. Senator who lauds himself as a representative of evangelical Christianity gave nothing to any church from 2006 to 2010. When asked the reason he replied that he and his banker wife were focused on building "a solid financial foundation to provide for my children." — Jim L. Wilson and Rodger Russell
The Week, February 12, 2016 p. 16
Malachi 3:8–10 (HCSB) "Will a man rob God? Yet you are robbing Me!" You ask: "How do we rob You?" "By not making the payments of the tenth and the contributions. 9 You are suffering under a curse, yet you—the whole nation—are still robbing Me. 10 Bring the full tenth into the storehouse so that there may be food in My house. Test Me in this way," says the LORD of Hosts. "See if I will not open the floodgates of heaven and pour out a blessing for you without measure.


text: Home Gallery Interactive (video) Marabou Tommy Nilsson Is Black Swedish Rock Star and Chocolate Bar Make Comebacks Together Candy brand Marabou Black ties its relaunch to singer Nilsson Published on May 06, 2013 Editor's Pick Swedish agency Forsman & Bodenfors has relaunched a classic Swedish candy brand, Marabou Black, by tying it in with the comeback of a forgotten 80s rock star. The brand, a licorice-flavored chocolate bar, discovered Tommy Nilsson (described by the agency as the "Michael Bolton" of Sweden), was planning a new single, so persuaded him to change his name on his Twitter account to "Black" and have a heavy-metal style makeover. The move generated a storm on Twitter, before Nilsson eventually revealed in a video that Marabou Black was behind it. The brand then became the number one trending topic on Twitter in Sweden, while both it and Nilsson generated national news headlines. Rate this Ad You must be registered to rate the ad. Please Login or Register Now About CreditsDateMay 06, 2013Agency:Forsman & BodenforsClient:Marabou TagsTwitter candy CategorySocial MediaStunt Latest Creativity News on Ad Age Why Are Verizon Execs Starring in Sprint's New Ad? How Storytelling Training Helped Georgia-Pacific Build Sales Without Boosting Budgets U.N. Staffers Petition to Recall Wonder Woman as Ambassador to Women Advertisement Ad Age Reports Partner White Paper 7 Tips for Keeping Your Creative Team Inspired Beat the creative slump with this informative guide which includes great advice on how to keep your team positive, productive and inspired. Brought to you by Shutterstock Premier. Learn more Related work Marabou: Onemansland Forsman & Bodenfors H&M: New Autumn Collection 2016 Forsman & Bodenfors Georg Jensen: You Can Never Be Too Much You Forsman & Bodenfors Want to see your work in our gallery? Submit now


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Agfa Graphics adds primer option to Jeti Titan S and HS flatbed inkjet printers
Thursday, May 01, 2014
Press release from the issuing company
The Jeti Titan HS made its North American debut at the International Sign Association's Sign Expo last week in Orlando. In May, it will also be shown at FESPA Digital in Munich, Germany. Its newly added primer option improves ink adhesion and extends the durability of prints.
"Compared to our other flatbed equipment, this machine uses far less ink to obtain the same, if not better, quality. The Jeti Titan HS will bring faster speeds, better quality and more output. It will be a great complement to our existing machinery for digital POP printing", said Gail Turner, Senior Vice President, Operations & Purchasing, Phase 3 Marketing & Communications. "On top of that, Agfa Graphics does an outstanding job with their service and support. We feel that they set the bar for service performance."
Jeti Titan S and HS come with Agfa Graphics' Asanti workflow for the sign and display market, which adds color management and automatic pre-flighting. This not only provides consistent quality but also minimizes errors, thus increasing productivity and saving time. Asanti is completed by the Asanti StoreFront cloud-based web-to-print system, which comes as an optional low-investment SaaS solution.
More about the Jeti Titan S and HS
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Toyota, Porsche post strong June salesTuesday, July 9, 2013
There is no shortage of reports indicating that the automotive industry saw strong sales in June. Both Edmunds and Kelley Blue Book reported that sales were above 1.3 million, capping off a strong first six months. However, some automakers fared better than others in June.
A recent report from Toyota showed that the automaker sold 195,235 units in June, up 14 percent on a year-over-year comparison. Of the sales, 173,880 were from the Toyota Division, up 14.4 percent on a year-over-year comparison. In addition, the automaker noted that sales in the first six months of 2013 were 6 percent higher than last year's robust pace during the same period of time.
"The auto industry led the economic recovery through the first half of 2013, kicking off a strong summer selling season, which we expect will carry into the second half of the year," said Bill Fay, Toyota Division group vice president and general manager. "Sales in June were solid, and demand didn't skip a beat. Customers are already showing an interest in the all-new Corolla, our hybrid lineup had its best-ever June and Camry topped 35,000 units."
The automaker's Lexus division sold 21,355 units, accounting for a 10.8 percent increase on a year-over-year comparison. However, Toyota was not the only carmaker with strong June sales.
Porsche has record June
A separate report from Porsche indicated that the automaker had its strongest June sales month in history, after it sold 3,700 vehicles.
"This first half of the year has been impressive on a number of fronts," said Detlev von Platen, president and CEO, Porsche Cars North America, Inc. "Along with experiencing record-setting deliveries, we were also recently recognized as the top brand in the annual J.D. Power Initial Quality Study, with our 911 and Boxster models ranking highest in their individual segments. In the context of an improving economy, we're encouraged to continue this momentum with a persistent cadence of new model launches this year."
The automaker noted that the Cayenne saw the largest growth last month, with sales jumping 77 percent on a year-over-year comparison. In addition, sales of the 911 were up 10 percent, as this is the 50th year the model has been available.
Whether you are buying a Toyota or a Porsche, any driver will benefit from equipping the interior of their vehicle with Husky Liners® car floor mats.


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Fender CD-140SCE
The new CD-140SCE is a brand new electro-acoustic guitar from Fender. This dreadnought cutaway offers tremendous value for money and aims to suit players from their beginner to intermediate stages.
A brand new electro-acoustic guitar from Fender, the CD-140SCE.
Upgrades include a new tortoise shell pickguard (natural finish model) or black pickguard (black finish model), new mother-of-pearl rosette design, rosewood bridge with compensated Graphtech Nubone saddle, aged bridge pins with black dots, mother-of-pearl Fender logo headstock inlay, and smaller (3 mm) dot fingerboard inlays. Features include scalloped "X"-bracing, 20-fret rosewood fingerboard, dual-action truss rod and Fishman Presys pickup system with active onboard preamp, tuner, volume and tone controls and low-battery indicator light.
- Body Shape - Dreadnought
- Bracing - Scalloped X
- Rosette - Fender Mother-of-Pearl Acrylic Design
- Body Material - Solid Spruce Top with Laminated Mahogany Back and Sides
- Body Finish - Gloss Polyurethane
- Body Back - Laminated Mahogany
- Body Sides - Laminated Mahogany
- Body Top - Solid Spruce
- Neck Material - Mahogany
- Neck Shape - "C" Shape
- Scale Length - 25.3" (643 mm)
- Fingerboard Radius - 11.81" (300 mm)
- Number of Frets - 20
- String Nut - Graph Tech NuBone
- Nut Width - 1.69" (43 mm)
- Neck Finish - Gloss
- Fingerboard - Rosewood
- Position Inlays - Pearloid Dot
- Special Electronics - Fishman Presys Pickup System with Active Onboard Preamp and Tuner
- Bridge - Rosewood with Compensated Graph Tech® Nubone™ Saddle
- Tuning Machines - Die-Cast
- Orientation - Right-Hand
- Pickguard - 1-Ply Black
See It In Action
Hear It In Action
Read About It
New: The 2017 Fender American Professional SeriesDecember 2, 2016Read Article
25 Years of Rock'n'Roll: The PMT TimelineSeptember 1, 2016Read Article
PMT Guide To Fender AmpsAugust 26, 2016Read Article
Fender UK Shops - PMT House of RockAugust 11, 2016Read Article
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This item is so popular, it has sold out. You're in luck! It'll be back soon. Please enter your email and we'll notify you as soon as it returns.
Expected: 03/16/2017
Table 19.7X31.5X15.7
Armchair 24X23.6X30.3
Bench 50.4X23.6X30.3
Upholstery Fabric - Polyester With Ca Foam
Weight: 81.4lb
Color: Grey Wash/Beige
Material: Acacia Wood
Style of Design: Modern


text:
Reuben Fernandes
4 Sentenced to Life in Keenan, Reuben Murder Case
The Mumbai Sessions Court pronounced four persons guilty in the Keenan-Reuben murder case and sentenced them to life on Thursday. On October 20, 2011 Keenan and Reuben were brutally stabbed to death outside the Amboli Kitchen and Bar in Mumbai after they stood up against eve teasers who were harassing their female friends.Arunoday Mukharji, May 5, 2016, 10:10 pm explore: India
Keenan, Reuben Get Justice After 5 Years
The Mumbai Sessions Court pronounced four persons guilty in the Keenan-Reuben murder case and sentenced them to life on Thursday. On October 20, 2011 Keenan and Reuben were brutally stabbed to death outside the Amboli Kitchen and Bar in Mumbai after they stood up against eve teasers who were harassing their female friends.Shawan Sen, May 5, 2016, 9:57 pm explore: India
Keenan-Reuben case: Charges likely to be framed
The two were attacked with choppers after they objected to tindecent behaviour against their women companions.October 22, 2012, 8:00 am explore: India
Keenan-Reuben case: Justice eludes Mumbai bravehearts
After the massive public outrage over their killing, the Maharashtra CM had promised a fast track court for the case. However, one year on, the case has progressed very slowly.Kajal Iyer, October 19, 2012, 7:12 pm explore: India
Keenan-Reuben case: Court to frame charges today
Four people were arrested for murder and sexual harassment and several others were booked for rioting in the casetest sharma, June 21, 2012, 8:21 am explore: India
Mumbai police forms squad on sexual harassment
The squad will mingle with crowds and constantly patrol colleges, malls and crowded streets.Kajal Iyer, November 9, 2011, 8:30 am explore: India
Keenan-Reuben case: accused face more charges
This has been done after the remaining two witnesses' statements were recorded.test sharma, November 8, 2011, 9:06 am explore: India
Eve teasing: Justice for Keenan and Reuben
Two young men, Reuben Fernandez and Keenan Santos, lost their lives fighting off men sexual harassing their friends.Smitha Nair, November 5, 2011, 10:02 pm explore: India
Mumbai murders case to be fast tracked: Patil
A metropolitan court remanded the four main accused to judicial custody till November 18.kajal Iyer & Yogita Limaye, November 4, 2011, 9:14 pm explore: India
Mumbai murders: accused in custody till Nov 18
Keenan, who died on the same day of the tragedy, was the first hit by the rowdy goons.Kajal Iyer, November 4, 2011, 2:03 pm explore: India
The Santos & Fernandes families - bravehearts left behindKajal Iyer, November 3, 2011, 11:56 pm explore: India
Ready to face Reuben's killers: Brother
Mumbai braveheart Reuben's brother Benjamin says if such criminals are not stopped, no woman will be safe.test sharma, November 3, 2011, 11:15 pm explore: India
Mumbai bravehearts killing: Accused in custody
The police custody of four accused, who were produced in the court on Thursday, were extended for a day.test sharma, November 3, 2011, 7:16 pm explore: India
Mumbai: Man who lost braveheart son stands unmoved
I have another 2 sons and I wish them also to be brave and to never bow down in front of this, Keenan's father said.test sharma, November 3, 2011, 8:19 am explore: India
Mumbai blamed for the killing of bravehearts
Angry friends of Mumbai bravehearts ask why Mumbaikars stood as mute spectators when the two were knifed to death.Kajal Iyer, November 2, 2011, 7:56 pm explore: India


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All SMU Adventurers
All Adventures, By Month
This Just In…
- Top 10 Things I Will Miss about Copenhagen
- A Week in the Life of a DIS Student
- Oslo, Bergen, and Barcelona, Oh My!
- Mental Health Abroad: You are Capable.
- A Love Letter to Kosovo
- Snapshots from Stockholm
- Overcoming Homesickness While Studying Abroad: Five Ways
- Touristy Tuesday: Abigail's Guide to Kronborg (AKA Hamlet's Castle)
Where Are They?
SMU students are having amazing adventures. Click on a continent to get started.
Daily Archives: August 11, 2014
I am woman, hear me roar
As I'm coming to the conclusion of my fellowship, I am growing increasingly attached to Nexus and the people there. However, I am grateful my time does not end here. I will be continuing my research beyond the fellowship, and … Continue reading
Posted in Nazia, Maguire Fellow in Dallas Comments Off on I am woman, hear me roar


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ActiveX Bridge for JavaBeans [ARCHIVE]
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1 Reply
Latest reply
on Dec 30, 2009 9:18 AM by 843804
Registering an ActiveX Bean control...
Mar 10, 2009 3:42 PM
Dear All,
I've done a little searching around for people in similar situations, but all I could find were a number of people with similar problems, alas without solutions.
Here we go:
I've followed the steps outlined here (http://java.sun.com/j2se/1.4.2/docs/guide/beans/axbridge/developerguide/index.html) to build a Bean and wrap it up into an ActiveX DLL.
I have managed to build the DLL, but when it gets to the end of the compilation process (or by using regsvr32), it comes up with:
"Registration failed:
The packages JavaBean is not located under the directory <jre_home>\axbridge\bin"
My environment is configured thus:
JRE_HOME=C:\Program Files\Java\jdk1.6.0_11
The DLL is inserted in:
C:\Program Files\Java\jdk1.6.0_11\axbridge\bin
and is located alongside the original jar.
Also, to add to the mix, this is being performed on a Vista machine, and I have tried running this as an administrator.
Any help would be much appreciated.
This content has been marked as final.
Show 1 reply
Re: Registering an ActiveX Bean control...
Dec 30, 2009 9:18 AM
in response to
You need to insert the DLL in C:\Program Files\Java\jre1.6.0_11\axbridge\bin
instead of C:\Program Files\Java\jdk1.6.0_11\axbridge\bin
Alternatively you can insert the DLL in C:\Program Files\Java\jdk1.6.0_11\jre\axbridge\bin


text:
Because Knetbooks knows college students. Our rental program is designed to save you time and money. Whether you need a textbook for a semester, quarter or even a summer session, we have an option for you. Simply select a rental period, enter your information and your book will be on its way!
From the author of The Graham Cracker Plot, a story about two friends playing finders keepers for the missing loot of Al Capone.
Christa spends every summer at the most awesome place in the whole world: her family's cabin on Whitefish Lake in Wisconsin. Only her dad recently lost his job and her parents have decided to sell the cabin. But not if Christa can help it. Everyone knows there is Al Capone blood money hidden somewhere in Whitefish Lake, and her friend Alex's cranky grandpa might have the key to finding it. Grumpa says the loot is gone, or worse-cursed!-but Christa knows better. If she finds it, she can keep it and save her family and their beloved cabin.


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Food, drink and entertainment: Self Catering accommodation. The HSM Torrenova Playa Apartments offers bar service at the pool during the day, with basic meals, snacks and all kind of drinks. Nearby there are countless bars, restaurants and shops.
Additional: 24-hour reception, multilingual staff, currency exchange, high chairs in the restaurant, at no extra cost, mini market, elevator, air conditioning, safe, car rental, bike rental, laundry, medical service, Internet access, Wi-Fi, major credit cards accepted. This property is not recommended for people with walking difficulties.
Sports, games and activities: Swimming pool for adults, terrace with sun beds and sunshades, children's pool, TV room, arcade room, plenty of opportunities to practice water sports on nearby beaches. Golf lovers will enjoy the various golf courses close by including Golf Poniente (2 km away), Real Golf Bendinat or Golf Santa Ponsa.
The HSM Torrenova Playa Apartments feature studios and apartments, all with spectacular sea views. All rooms (both studios and apartments) have a bathroom, air conditioning, safety deposit box (extra charge), telephone, Satellite TV and spacious terrace. Roomy apartments can accommodate up to 4 people and consist of a bedroom and a living room with equipped kitchen. Studios can accommodate up to 3 people and offer a living room combined with a kitchen, equally equipped as apartments, but without a separate bedroom. Free baby cot available on request.
We reserve the right to refuse and claim where we are unable to substantiate the
claim to our satisfaction and in accordance with the above points. We reserve the
right to remove this offer at any time.
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- Details
The Kendama - 5 inch, Plastic, Light-up is an awesome twist on the classic toy from Japan. It's made of colorful plastic and a glitter ball that lights up! Turn off the lights for a fun new way to play. Try catching the ball on the handle of this Kendama Game. Playing kendama can help you to develop your coordination skills and it gets you up and out of your office chair! Take some time out from your busy day to exercise and play with this cool handheld game. The Kendama - 5 inch, Plastic, Light-up handle is approximately 4.8" long. The ball is approximately 2" diameter, ball is attached to handle with a 13" long cord.
Additional Information
- Dimensions: Handle is approximately 4.8" long. The ball is approximately 2" diameter, ball is attached to handle with a 13" long cord
- Material: Plastic
- Color/Style: Blue, Green, Purple, Red
- WARNING: CHOKING HAZARD - Small parts. Not for children under 3 yrs.
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Shipping is based on your total order weight, your shipping method, and your shipping destination. Standard shipping to the United States starts at only $4.99! Visit your shopping cart and use 'estimate shipping and tax' to see available shipping rates.
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Delivery time depends on the shipping method you select and your distance from our distribution center in Northern California. Typical transit time to the western United States using Standard Shipping is 2-4 business days. Typical transit time to the eastern United States using Standard Shipping is 5-7 business days. International shipments typically take 6-12 business days. Expedited shipping methods are also available for faster arrival.
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This is a long gold tone metal chain necklace. It features three graduated lengths of gold tone metal chains with large textured links. The vertical drop is 20" on the longest strand and 18" on the shortest. It is in excellent condition and looks fabulous on!
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Eclectic Vintage Collectibles ~ Purses ~ Jewelry ~ Glass ~ Books ~ Toys ~ Ephemera & Holiday Items
Thanks to everyone for making Vintage Vault one of Ruby Lane's "Top 50" Most Visited Shops! Happy Holidays!


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1. R. Expo USA / Song of India |
Auburn, Washington United States
Supplier of Fragrances, Perfume Oils, Incense, Burners, Candles, Handicrafts, Wood & Stone Boxes, Hindu & Buddhist Figurines, Body Oils, Soaps, Shampoos, Jewelry, Scarves & More from India. Since 1932. We provide high quality products and great customer service!
2. East Artisan |
Lawrenceville, New Jersey United States
We are a wholesale supplier of incense, perfumes, body oils, and over 8000 fragrances!!!
3. Astral Sea Fragrance / JMW I LLC |
Salem, Missouri United States
Made in the USA. High quality oils, incense sticks, cones & powders (packaged and bulk) Bath Salts, Perfume Creams, Body Sprays, Fragrance Oils and more. When you choose Astral Sea you can be confident they are the freshest. Home of the original Fairy Farts. Over 25 years of The Finest Fragrance in
4. Mato Natural |
Lawrenceburg, Kentucky United States
Mato Naturals are producers of Eco-friendly natural organic products ranging from household goods,health & beauty to fashion & accessories. We manufacture Eco-friendly products made from sustainable raw materials such as hemp, bamboo, allo, natural extracts etc. Few of our top selling products are w
5. Reach for the Sales
Brooklyn, New York United States
Reach for the Sales is a closeout general merchandise supplier with an expertise in sourcing from major suppliers both in and out of the U.S. Our products are meant to target companies who deal with e-commerce and who have discount retail stores however, it doesn't stop there. Reach for the Sales.
South Hackensack, New Jersey United States
*Free Shipping* *15% Discounts* *New Products Weekly* *K Beauty brands and skincare* *Organic Product Lines* *Best Fashion* *Same Day Shipping* *Professional Customer Service*
7. AKR Corp.
Bayshore, New York United States
AKR Corporation is a leader in wholesale and distribution of Health & Beauty Aids and General Merchandise. Competitive pricing, extensive variety and superb service, since 1985.
8. International Fragrance Shop |
Baltimore, Maryland United States
International Fragrances Inc. is an Importer, Distributor, and Wholesaler of the Premium Grade Fragrance Oils, Essential Oils, and Aroma Diffusers. International Fragrances is a Family Owned Company. We believe in Quality and Honesty. We always do our best to satisfy the needs of our customers.
9. Natural Leigh, LLC
Salem, Oregon United States
At Natural Leigh, we offer a range of products that have been developed from all natural ingredients to produce the finest solutions for everything from all natural pain relief to weight loss and even beauty. Our FDA inspected processing facility maintains some of the highest quality standards.
10. 3rd Phaze Body Oils |
Charlotte, North Carolina United States
Over 1500 different Body Oil Fragrances. We also carry Incense, Aroma/Burning oils, Electric Lamps, Ceramic/Polyresin. We have over 100 different soaps also.
11. Merchandize Liquidators
Hallandale, Florida United States
Merchandize Liquidators is a cosmetics and general merchandise closeout supplier, offering overstock and shelf pulled makeup lots from many drug stores in the country as well as overrun and surplus directly from the distribution centers.
12. AFG Distribution |
Asheville, North Carolina United States
We offer a variety of products to alternative retailers: Vaporizers, Water Pipes, Grinders, Hookahs, Scales, Dresses and Skirts, Glass Jars, Hemp Jewelry, Detox Items, Incense, Pipe Pouches, Smoking/Tobacco Accessories, Drinking Games, Posters, Tapestries, Music Memorabilia, and Novelties.
13. Odontomed2011.com
Miami, Florida United States
Renowned for its Medical Instruments, to make the steel into useful tools of the highest quality. Surgical Instruments, Scissors, Medical Tools, Manicure Instruments and Dental Tools.
14. FragranceNet.com |
Deer Park, New York United States
Trusted since 1997 FragranceNet.com carries over 18,000 genuine brand named fragrances, skin care, hair care, aroma therapy & candles all at wholesale prices up to 70% off retail. Drop shipping available no fees.
15. North and South Wholesalers |
Miami Gardens, Florida United States
High quality brand name products! HBA, Gen. Merch, Housewares, dollar store! 1000's of products, lowest prices.
16. LA Fragrances
Los Angeles, California United States
Wholesellers of branded fragrances, Skincare and Color Cosmetics.
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|Publication number
|Publication type
|Application number
|Publication date
|Nov 28, 1978
|Filing date
|Jun 10, 1977
|Priority date
|Jun 10, 1977
|Also published as
|Publication number
|Charles M. Li Mutti, Tai-Wing Wu, Shirley Y. Lynn
|Original Assignee
|Eastman Kodak Company
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (6), Referenced by (77), Classifications (12), Legal Events (1)
|External Links: USPTO, USPTO Assignment, Espacenet
(1) Field of the Invention
This invention relates to a serum matrix or serum-derived composition containing lipids, such as a lipid calibrator, which has been stabilized against inhomogeneity which results from lyophilization and reconstitution with water.
(2) State of the Prior Art
Automated clinical analyzers such as those which rely upon radiometric detection of a change in absorption require calibration using a "standard" calibrator. Such calibrators permit two or more known concentrations of an analyte of choice to be fixed at particular levels, e.g., color densities, as read by the analyzer, and from these a curve is derived for use with readings of unknown analyte concentrations.
For example, lipid calibrators are used for calibrating the analysis of cholesterol and triglycerides. Such calibrators, as with other calibrators, are either prepared fresh on a daily basis, or as is more common, are stored in a lyophilized condition for reconstitution at a later date. It is well-known that lipoproteins will not readily tolerate lyophilization, but rather produce an inhomogeneous, turbid reconstituted solution, due to instability of particular lipoproteins to the lyophilization process. Serum and blood plasma containing lipoproteins and glucose in naturally occurring amounts have also demonstrated unacceptable turbidity after lyophilization and reconstitution.
U.S. Pat. Nos. 3,260,648 issued on July 12, 1966 and 3,955,925 issued on May 11, 1976 are illustrative of the problem. As explained in these patents, turbidity interferes with the analysis and therefore with the calibration of the analyzer. Although turbidity might be overcome by many-fold dilutions, such dilutions are time-consuming and therefore undesirable.
U.S. Pat. Nos. 3,955,925 and 4,011,045; and Japanese Patent O.P.I. No. 144724/76 are of interest, because they disclose that turbidity upon lyophilization and reconstitution can be avoided, by removing, such as by salting out, most of the lipoproteins which are the primary cause of the turbidity problem. Thereafter, desired proteins such as α-fetoprotein, which is not a lipoprotein, or glycerides of low molecular weight fatty acids, are added back. In the Japanese process, lactose is described as a useful additive for the lyophilization step, presumably for the bulking effect described hereinafter. The technique described in U.S. Pat. No. 4,011,045 however is seemingly of limited utility, being disclosed as a method for preparing a useful standard only for triglycerides.
Because fresh, daily preparation of the calibrator is usually impractical as an alternative to lyophilization and reconstitution, what is needed is an additive that will permit the reconstitution of lyophilized lipid calibrators without a significant loss of optical clarity.
Sugar and sugar derivatives have been used as bulking agents and stabilizers for reconstituted, lyophilized assay mixtures, as shown for example in U.S. Pat. No. 3,413,198. However, the assay mixtures described in this patent are not disclosed specifically as containing lipids such as cholesterol or triglyceride, and no recognition is given to an improvement in clarity to be achieved by the use of such "bulking agents."
Lactose has been used as a bulking agent for solutions in general to be lyophilized, but only where the solutions require such bulk for ease in the lyophilization process. However, the amounts of lactose used for this effect generally have been less than that found to be effective by this invention to provide clarity. Moreover, lipoproteins due to their large molecular size do not require such additional bulking agents.
Glucose is of course a sugar that is both naturally present in serum or has been added to calibrators of various kinds. However, the amount present either naturally or by addition has been ineffective to produce any clarity stabilization, as is evident from the fact that human serum, upon lyophilization, typically exhibits a drastic increase in turbidity.
Patents which relate generally to the background of lipids or other calibrators include U.S. Pat. Nos. 3,274,062; issued on Sept. 20, 1966; 3,751,381 issued Aug. 7, 1973; 3,897,363 issued July 29, 1975; and 4,007,008 issued Feb. 8, 1977.
It is an object of this invention to provide a lipid-containing serum matrix or serum composition which upon lyophilization and reconstitution has a homogeneity which is superior to that which results in such processing of untreated serum or serum matrices.
It is another object of the invention to provide such a matrix or composition with a homogeneity, at the user's option, which is comparable to that of the matrix or composition prior to lyophilization.
It is a related object of the invention to provide a lipid calibrator with a homogeneity as described above, even after lyophilization and reconstitution.
Other objects and advantages will become apparent upon reference to the following Summary and Detailed Description.
The invention concerns a serum matrix or serum-derived composition which, upon lyophilization and reconstitution, has a homogeneity that is superior to what is obtained from lyophilization and reconstitution of untreated serum matrices and serum-derived compositions. Such improved homogeneity is readily measured in terms of the reduced optical density of the reconstituted matrix or composition.
More specifically, in accordance with one aspect of the invention there is provided a stabilized serum matrix or serum-derived composition comprising lipids and a clarity stabilizer comprising a sugar, sugar amine, or sugar alcohol in at least an amount sufficient to reduce the optical density of the composition, after lyophilization and reconstitution with water, below the optical density of said lyophilized and reconstituted matrix or composition lacking said stabilizer, when measured at 700 nm.
This composition has a preferred utility as a lipid calibrator.
There is further provided, by this invention, a method of maintaining homogeneity in a lipid-containing serum matrix or serum-derived composition even after lyophilizing and reconstituting, comprising the steps of
(a) adding to said matrix or composition prior to lyophilization a clarity stabilizer comprising a sugar, sugar amine, or sugar alcohol in at least an amount sufficient to reduce the optical density of the composition, after lyophilization and reconstitution with water, below the optical density of said lyophilized and reconstituted matrix or composition lacking said stabilizer, when measured at 700 nm; and
(b) thereafter lyophilizing said matrix or composition;
whereby upon reconstitution with water said matrix or composition has a reduced amount of turbidity.
Serum matrices and serum-derived compositions, which include serum as well as plasma, can be stored in powdered form. However, the lyophilization and reconstitution process may introduce substantial amounts of inhomogeneity. Such inhomogeneity is conveniently measured in terms of the turbidity of the liquid sample, and significant departures from optically clear levels are deemed to be evidence of such inhomogeneity. As used herein, "optically clear" means having an optical density no greater than about 0.5. All optical densities herein referred to are measured at a wavelength of 700 nm, a wavelength at which inherent absorption capabilities of serum will not interfere, and a 1 cm. path length.
It has been discovered that by the use of clarity stabilizers, serum matrices and serum-derived compositions can be lyophilized and reconstituted with a retention of homogeneity beyond that available for matrices and compositions not processed in accordance with the invention. Such serum matrices or serum-derived compositions of the invention can be in liquid or lyophilized form, and if liquid, can be derived or reconstituted from the lyophilized form.
Thus, in one form of the invention a clarity stabilizer is added to the serum matrix or serum-derived composition in at least an amount which is sufficient to measurably reduce the optical density, and therefore the inhomogeneity, measured after lyophilization and reconstitution, to a level below that which would occur in the absence of the stabilizer. As used herein, "measurably reduce" means a reduction in optical density of at least about 0.5.
In another form of the invention, the amounts of stabilizer can be increased to levels which produce an optical density, and therefore a homogeneity, that is comparable to that of the freshly prepared matrix or composition, in liquid form, prior to lyophilization. As used herein, "comparable" means producing an optical density which is increased, if at all, compared to the unlyophilized matrix or composition lacking the stabilizer, by no more than about 0.5, measured at 700 nm.
In still another form of the invention, the clarity stabilizers are present in maximum amounts which provide a matrix or composition, after lyophilization and reconstitution, that is optically clear, i.e., has an absolute optical density no greater than about 0.5, at 700 nm.
The particular level of optical density, and therefore homogeneity, that is selected from among the aforedescribed three levels will depend of course on the amount of inhomogeneity that the user can tolerate in his clinical analysis procedure. Analysis procedures most sensitive to turbidity and inhomogeneity may require the use of an optically clear matrix. But even the most insensitive procedures can benefit by the measurable reduction in optical densities achievable by this invention.
Although hereinafter described embodiments of the invention are directed to the preferred utility as a calibrator, other uses are contemplated. For example, the invention provides a means of converting fresh serum or a serum-derived composition to dry form for purposes of storage and then reconstituting it without substantially affecting the homogeneity of the fresh serum. The reconstituted serum matrix or serum-derived composition can then be used in any of a variety of different medical or clinical applications.
As is apparent, the optical density levels after reconstitution depend in part on the amount of water used. As used herein, "reconstitution" means the addition of water in an amount sufficient to restore the volume to what it was just prior to lyophilization, with no further dilutions. Thus, the amount of water used in the reconstitution is fixed by the amount that was present before.
The preferred utility of such compositions is as a lipid calibrator for clinical analysis. Also, for other assays, other analytes can be present in the calibrator in addition to lipids, as long as the other analytes and the clarity stabilizer are compatible, i.e., the analytes are not attacked or otherwise rendered undetectable in the assay of choice, by the clarity stabilizer. Thus, analytes such as blood urea nitrogen, electrolytes such as potassium and chloride, bilirubin, amylase, and the like can be present where compatible with the clarity stabilizer of choice. Such additional analytes are preferred and conventional where the calibrator is intended to be a broad reference calibrator for a number of different individual analytes. Of course, if the calibrator is being used in an analysis for glucose, a clarity stabilizer other than glucose would normally be selected.
Clarity stabilizers which have been found to be effective in this invention include sugars, sugar alcohols and sugar amines. Useful examples of these classes include glucose, sucrose, lactose, arabinose, sorbitol, fructose, xylitol, mannitol, and glucosamine. It is further contemplated that any sugar, sugar alcohol, or sugar amine apart from those just named will also be effective if used in appropriate amounts. Of course, with some sugars or sugar derivatives, there is an upper limit as to the amount that can be used, as excess amounts become insoluble. As will be appreciated, the level at which insolubility occurs depends upon the particular stabilizer and matrix to which it is added.
The amount which will render the clarity of the reconstituted composition comparable to that of the material prior to lyophilization depends largely upon which clarity stabilizer is selected. Typically, an effective amount has been found to range between about 2.5 to about 30 grams per deciliter of solution. It will be appreciated that these amounts can vary slightly depending upon the level of lipids, or upon further optimization of the matrix. Also, the amounts selected can vary slightly depending upon the amount of protein.
The stabilizers described above can be used to stabilize any lipid-containing composition which is to be lyophilized and later reconstituted. Highly preferred uses feature the addition of the stabilizers to human serum matrices for any use in general, and for lipid calibrators in particular.
The present invention is applicable to a serum matrix or serum-derived composition obtained from any of a wide variety of sources, including conventional calibrator suppliers such as commercially available cholesterol concentrates or pooled human serum. The lipids can be obtained from these sources, or from nonserum sources such as egg yolk. A useful technique in preparing calibrators comprises column separation of lipids from pooled human serum by exclusion chromatography followed by further concentration of the lipid-containing column fractions using ultrafiltration, optional dilution of the concentrate to lower lipid values, if desired, and optional dialysis to replace the eluant fluid with a salt solution of desired constituents. More specifically, column separation comprises loading conventional gel permeation chromatographic columns with human serum from any source, and using a buffered solution to elute the large lipoproteins from the serum while retaining albumin in the column. A buffered solution is preferred because of the possibility of denaturing the lipoproteins if the pH is allowed to vary drastically. Typical buffered solutions which are useful include those having a pH between about 7 and about 8, such as 0.1 M Tris buffer or Ringer's solutions prepared with this specific pH. The desired lipid-containing eluant coming off the column may still be too low in lipid concentration, about 50 to 100 mg/dl total cholesterol, so that further concentration may be desired for the higher lipid levels needed in calibrators. A highly preferred concentration technique includes conventional ultrafiltration using for example an ultrafiltration cell equipped so that molecules with a molecular weight greater than 5000 daltons are retained.
Detection of the presence and/or amount of lipids in the lipid-rich fractions from the column can be done by any conventional assay technique, such as the Liebermann-Burchard or the Zak assays. Alternatively, the cholesterol detection element described in Research Disclosure, Vol. 126, October 1974, No. 12626, published by Industrial Opportunities Limited, Homewell, Havant Hampshire PO91EF, United Kingdom, can be used.
Upon reaching the highest lipid concentration desired, dilutions to lower concentrations for a multipoint calibrator can be achieved by adding the stock concentrate to pooled human serum, if desired. Furthermore, the level of proteins can be adjusted, if desired, by adding back gamma globulin and/or albumin.
Dialysis of the resultant lipid-containing liquid is a further optional and conventional processing step, particularly if the eluant does not contain the desired salts. A Ringer's solution or any isotonic salt solution, containing sodium chloride and optionally other ions such as potassium, is particularly useful for this purpose. Such other ions are desirable in preparing, for example, an ion-selective electrode calibrator.
The invention can be further illustrated by the following representative examples. All optical densities (O.D.) expressed herein are in terms of the relationship O.D. = log Io /I, wherein Io is the intensity of the incident ray and I is the intensity of the transmitted ray. The wavelength of the incident ray was, in all instances, 700nm.
The stabilizers listed in Table I were added at various levels to aliquots of column-generated lipid concentrate containing about 500mg/dl total cholesterol, about 450 mg/dl triglycerides and about 4 g/dl total protein containing essentially no albumin. This concentrate was prepared by eluting lipid-rich fractions from human serum added to a 30 l. Pharmacia column filled with Sephadex G-200, the eluant being 0.1 M Tris buffer containing 0.1 M sodium chloride. After elution, the fractions were combined and ultrafiltered using a filter having a molecular weight cutoff at 5000 daltons, and dialyzed against a physiologic saline solution containing 8.5 g/dl NaCl. The examples and a control containing no stabilizer were lyophilized and subsequently reconstituted with distilled water. A measure of turbidity was obtained by noting the optical densities of the reconstituted examples on a Beckman 25 Spectrophotometer, at 700nm. Results are shown in Table I, the control being shown both before and after lyophilization and reconstitution.
Table I__________________________________________________________________________ Stabilizer AmountExample Stabilizer (g/dl) Optical Density__________________________________________________________________________Control-unlyophilized none 0 0.50Control-lyophilized & reconstituted none 0 >2.91 - lyophilized & reconstituted arabinose 2.5 2.5 arabinose 5.0 1.08 arabinose 15.0 0.62 - lyophilized & reconstituted sorbitol 2.5 2.6 sorbitol 5.0 1.53 sorbitol 15.0 0.523 - lyophilized & reconstituted sucrose 2.5 2.7 sucrose 5.0 1.63 sucrose 15.0 0.514 - lyophilized & reconstituted glucosamine 2.5 2.6 glucosamine 5.0 1.34 glucosamine 15.0 1.15__________________________________________________________________________
Table I demonstrates that, for the matrix tested, the amounts required for comparable clarity were, for arabinose, about 5 g/dl; for sorbitol and for sucrose, about 10g/dl, and for glucosamine, about 15 g/dl. The amounts required to create an optically clear standard, found to exist in the control prior to lyophilization, were, for arabinose, sorbitol, and sucrose, about 15 g/dl.
The procedures of Examples 1-4 were repeated, except that the serum matrix was pooled human serum obtained from Interstate Blood Bank, which contained about 390 mg/dl triglycerides, 219 mg/dl total cholesterol, and about 7 g/dl protein as determined by the Biuret procedure, of which at least about half was albumin. The concentrations of the stabilizers were varied as shown in Table II, and the optical densities measured after lyophilization and reconstitution only, except for the control which was measured before as well as after lyophilization and reconstitution.
TABLE II__________________________________________________________________________ Stabilizer AmountExample Stabilizer (g/dl) Optical Density__________________________________________________________________________Control-unlyophilized none 0 0.396Control-lyophilized & reconstituted none 0 >2.505 - lyophilized & reconstituted glucose 5.0 >2.50 glucose 10.0 0.808 glucose 20.0 0.2956 - lyophilized & reconstituted lactose 5.0 >2.50 lactose 10.0 insoluble7 - lyophilized & reconstituted arabinose 5.0 >2.50 arabinose 10.0 0.697 arabinose 20.0 0.2678 - lyophilized & reconstituted mannitol 10.0 >2.50 mannitol 20.0 insoluble9 - lyophilized & reconstituted fructose 5.0 >2.50 fructose 10.0 0.941 fructose 20.0 0.467 fructose 30.0 0.17410 - lyophilized & reconstituted glucosamine 10.0 >2.50 glucosamine 20.0 insoluble__________________________________________________________________________
These results demonstrated that, for the matrix tested, the amounts of glucose, arabinose, and fructose necessary for clarity comparable to that of the unlyophilized control were about 10 g/dl. Glucose, arabinose and fructose all produced optically clear standards useful as lipid calibrators when 20 g/dl were used. In fact, both arabinose at 20 g/dl and fructose at 30 g/dl demonstrated after reconstitution a clarity that was superior even to the clarity of the matrix prior to lyophilization.
The failure in these examples of lactose, mannitol, and glucosamine, at these levels, particularly when compared with Examples 1-4, most likely can be explained on the basis of the increased protein levels of this matrix.
The following example demonstrates that the reductions in optical densities obtainable by the invention are in fact a measure of a lack of inhomogeneity of the type evidenced by turbid, lyophilized and reconstituted lipoprotein materials available from prior art techniques.
Analytical data was obtained on the serum components cholesterol, glucose, total proteins and uric acid from pooled human serum that was (1) fresh, (2) lyophilized without a clarity stabilizer and (3) lyophilized with 5 g/dl sorbitol and reconstituted. Table III shows that with the additive the matrix or composition provides a highly repeatable analysis compared to the lyophilized matrix without an additive, such as is desirable for a calibrator.
Table III__________________________________________________________________________Assay Values of Selected Serum Analytes in a Human Serum Pool Stabilized Matrix Untreated Matrix (5 g/dl Sorbitol) Unlyophilized Lyophilized and Reconsti- Lyophilized and Reconstituted Control tuted Without Stabilizer With Water Average Average Average mg/dl Std Dev mg/dl Std Dev mg/dl Std DevAnalyte Assayed1 (n=3) (σ) % COV2 (n=3) (σ) % COV (n=3) (σ) % COV__________________________________________________________________________Total Cholesterol 205.53 0.58 0.28 203.3 14.47 7.12 199.3 0.58 0.29(Gilford)Glucose 136.3 3.79 2.78 136.3 1.15 0.85 138.33 0.58 0.43(ACA DuPont)Total Protein 6.40 0.00 0.00 6.25 0.01 0.09 6.8 0.0 0.0(ACA)Uric Acid 6.73 0.06 0.86 6.47 0.21 3.22 6.67 0.15 2.29(ACA)__________________________________________________________________________ 1 The name within the parentheses indicates the assay used. 2 Throughout this table, COV stands for Coefficient of Variation.
The invention has been described in detail with particular reference to certain preferred embodiments thereof, but it will be understood that variations and modifications can be effected within the spirit and scope of the invention.
|Cited Patent
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|Aug 20, 1996
|Genzyme Corporation
|Compositions for stabilizing proteins for long term dry storage and methods of making and using the compositions
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|Apr 15, 1997
|Quadrant Holdings Cambridge Limited
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|Feb 9, 1996
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|Method for stabilization of biological substances during drying and subsequent storage and compositions thereof
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|Sep 28, 1999
|Quadrant Holdings Cambridge Ltd
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|Sep 18, 2001
|Quandrant Holdings Cambridge Limited
|Solid dose delivery vehicle and methods of making same
|Sep 3, 1999
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|Quardrant Holdings Cambridge, Ltd.
|Method for stabilization of biological substances during drying and subsequent storage and compositions thereof
|Aug 1, 2000
|Dec 18, 2001
|Quadrant Holdings Cambridge Limited
|Solid dose delivery vehicle and methods of making same
|Jul 23, 1997
|Sep 24, 2002
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|Compositions for use in rehydration and nutrition during athletic exercise and methods of making same
|Jan 26, 2001
|Mar 4, 2003
|Trinity Biotech Manufacturing, Ltd.
|Methods for quality control of prothrombin time (PT) and activated partial thromboplastin time (APTT) assays using coagulation controls
|Aug 31, 2001
|May 20, 2003
|Elan Drug Delivery Ltd.
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|Elan Drug Delivery Limited
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|Jun 3, 2002
|Jul 22, 2003
|Elan Drug Delivery Limited
|Compositions for use in rehydration and nutrition during athletic exercise and methods of making same
|May 14, 1996
|Oct 14, 2003
|Elan Drug Delivery Limited
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|Oct 25, 2002
|Nov 2, 2004
|Quadrant Drug Delivery Ltd.
|Solid dose delivery vehicle and methods of making same
|Apr 16, 2001
|May 10, 2005
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|Feb 27, 2003
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|Sep 4, 1997
|Nov 15, 2005
|Elan Drug Delivery Limited
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|Aug 29, 2003
|Jun 6, 2006
|Quadrant Drug Delivery Ltd.
|Solid dose delivery vehicle and methods of making same
|Sep 18, 2002
|Nov 27, 2007
|Nektar Therapeutics
|Pulmonary delivery of active fragments of parathyroid hormone
|Mar 12, 2002
|Dec 11, 2007
|Nektar Therapeutics
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|Feb 2, 2005
|Apr 1, 2008
|The Research Foundation Of State University Of New York
|Compositions and methods for less immunogenic protein formulations
|Jul 1, 2003
|Apr 21, 2009
|Novartis Ag
|Methods and compositions for pulmonary delivery of insulin
|Aug 19, 2003
|Dec 8, 2009
|Novartis Pharma Ag
|Stabilized preparations for use in metered dose inhalers
|Aug 9, 2006
|Feb 16, 2010
|The Research Foundation Of State University Of New York
|Compositions and methods of preparation of liposomal microparticulate IL-12
|May 20, 2005
|Jun 29, 2010
|Quadrant Drug Delivery Limited
|Solid dose delivery vehicle and methods of making same
|May 20, 2005
|Aug 24, 2010
|Quadrant Drug Delivery Limited
|Solid dose delivery vehicle and methods of making same
|May 20, 2005
|Aug 31, 2010
|Quadrant Drug Delivery Limited
|Solid dose delivery vehicle and methods of making same
|May 10, 2000
|Jan 18, 2011
|Novartis Ag
|Stable metal ion-lipid powdered pharmaceutical compositions for drug delivery and methods of use
|Mar 30, 2007
|Jan 25, 2011
|The Research Foundation Of State University Of New York
|Method for treating blood coagulation disorders
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|Feb 7, 2012
|The Research Foundation Of State University Of New York
|Compositions and methods for less immunogenic protein-lipid complexes
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|May 1, 2012
|Novartis Pharma Ag
|Engineered particles and methods of use
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|Aug 21, 2012
|Novartis Ag
|Respiratory dispersion for metered dose inhalers comprising perforated microstructures
|Dec 14, 2010
|Jan 8, 2013
|Novartis Ag
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|Jan 8, 2013
|The Research Foundation Of State University Of New York
|Reconstitution medium for protein and peptide formulations
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|Mar 26, 2013
|Novartis Ag
|Formulation for pulmonary administration of antifungal agents, and associated methods of manufacture and use
|May 11, 2010
|Aug 27, 2013
|Biomatrica, Inc.
|Compositions and methods for biological sample storage
|Oct 24, 2008
|Apr 29, 2014
|Novartis Ag
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|Oct 31, 2007
|May 6, 2014
|Novartis Ag
|Pulmonary delivery of aminoglycoside
|Dec 6, 2012
|Nov 4, 2014
|Novartis Ag
|Stable metal ion-lipid powdered pharmaceutical compositions for drug delivery
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|Dec 2, 2014
|Biomatrica, Inc.
|Integration of sample storage and sample management for life science
|Jul 30, 2008
|Jul 14, 2015
|Biomatrica, Inc.
|Integration of sample storage and sample management for life science
|Jul 26, 2011
|Jun 28, 2016
|Biomatrica, Inc.
|Compositions for stabilizing DNA and RNA in blood and other biological samples during shipping and storage at ambient temperatures
|Apr 8, 2014
|Aug 23, 2016
|Novartis Ag
|Pulmonary delivery of aminoglycoside
|Apr 2, 2014
|Sep 13, 2016
|Novartis Ag
|Phospholipid-based powders for drug delivery
|Feb 27, 2003
|Aug 7, 2003
|Roser Bruce J.
|Solid dose delivery vehicle and methods of making same
|Aug 29, 2003
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|Roser Bruce J.
|Solid dose delivery vehicle and methods of making same
|Aug 5, 2004
|Mar 31, 2005
|Balasubramanian Sathyamangalam V.
|Reconstitution medium for protein and peptide formulations
|Mar 18, 2005
|Jul 28, 2005
|Elan Drug Delivery Limited
|Methods of preventing aggregation of various substances upon rehydration or thawing and compositions obtained thereby
|Mar 15, 2005
|Aug 25, 2005
|Elan Drug Delivery Limited
|Methods for stably incorporating substances within dry, foamed glass matrices and compositions obtained thereby
|Feb 2, 2005
|Oct 13, 2005
|Sathyamangalam Balasubramanian
|Compositions and methods for less immunogenic protein formulations
|Aug 9, 2006
|Mar 29, 2007
|Balu-Iyer Sathy V
|Compositions and methods of preparation of liposomal microparticulate IL-12
|Jun 29, 2006
|Jun 21, 2007
|Balu-Lyer Sathy V
|Compositions and methods for less immunogenic protein-lipid complexes
|Feb 20, 2007
|Oct 25, 2007
|Jaap Kampinga
|Methods of terminal sterilization of biological products
|Mar 30, 2007
|Nov 29, 2007
|Balu-Iyer Sathy V
|Compositions of less immunogenic and long-circulating protein-lipid complexes
|Mar 30, 2007
|Nov 29, 2007
|Balu-Iyer Sathy V
|Method for treating blood coagulation disorders
|Jul 28, 2008
|Feb 26, 2009
|The Research Foundation Of State University Of New York
|Compositions and methods for less immunogenic protein-lipid complexes
|Sep 5, 2008
|Mar 5, 2009
|The Research Foundation Of State University Of New York
|Reconstitution Medium For Protein and Peptide Formulations
|May 11, 2010
|Apr 7, 2011
|Biomatrica, Inc.
|Compositions and methods for biological sample storage
|Dec 28, 2010
|May 26, 2011
|Balu-Iyer Sathy V
|Compositions of Less Immunogenic and Long-Circulating Protein-Lipid Complexes
|Jun 18, 1980
|Jan 7, 1981
|IMMUNO Aktiengesellschaft f�r chemisch-medizinische Produkte
|Method for preserving the electrophoretic properties of lipoproteins and use of the preserved serums
|Jun 6, 1984
|Jan 9, 1985
|Gilford Instrument Laboratories, Inc.
|Stabilized clinical control reagents
|Sep 29, 1999
|May 3, 2000
|Dade Behring Marburg GmbH
|Stabilisation of biological fluids by addition of sterol esters
|Aug 5, 2004
|Jun 14, 2006
|The Research Foundation Of State University Of New York
|Reconstitution medium for protein and peptide formulations
|Feb 21, 1995
|Aug 24, 1995
|Genzyme Corporation
|Protein calibrator/control product
|U.S. Classification
|International Classification
|Cooperative Classification
|European Classification
|Apr 28, 1995
Owner name: CLINICAL DIAGNOSTIC SYSTEMS INC., NEW YORK
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:EASTMAN KODAK COMPANY;REEL/FRAME:007453/0348
Effective date: 19950118


text: I've gone over it so many hundreds of times to the point of annoyance. I've been trying to get Remote to work, but it won't. It just WON'T. My iPod is a 1st Gen. 32GB on 3.0 Jailbroken. Every time I try to connect it, the little Remote icon doesn't show up under Devices in iTunes. Having this app work for me would be the most convenient thing for my sound setup to be perfect! Remote won't show up under Devices when I'm plugged in wire or wireless. I've installed Bonjour, made sure it, along with iTunes, passes BOTH of my firewalls, and also have the correct options in iTunes, such as library sharing, scanning for Remote devices, etc. Is there ANYTHING else I can do, or is this app just a bust? I've Googled, BTW, and come up with NOTHING. Please Register or Log in to view images


text:
Herbed Mahi Mahi
Total Time
Prep 10 mins
Cook 15 mins
Wanted a quick dinner and checked through the garden, sure enough a little of this and a little of that....voila!
Ingredients Nutrition
- 1 lb mahi mahi
- 8 fresh basil leaves, rolled and sliced thin
- 2 tablespoons fresh garlic chives, minced (or regular)
- 2 tablespoons fresh parsley, chopped
- 2 tablespoons salsa, mild (your choice mild or hot)
- 1 tablespoon capers, drained
- 1 tablespoon olive oil
- Combine all ingredients.
- Spread on fish and bake at 350 until done.


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This is a fabulous early pin which dates to the 1930's. It is made of pot metal and features a steamboat covered in rhinestones. Black enameling is also present. The wheel moves and spins.
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Au Canada, les Samsung Galaxy Note 7 restants seront bientôt inutilisables
Galaxy s4 Mini Double Sim GT i9192
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Just Plain English No Clichés (or sex) Please
More than 5000 supporters in over 70 countries participated in a campaign to decide the most irritating phrases for the English language. At the end of the day the number one voted cliche was - you guessed it… "At the end of the day". Second place was shared by "At this moment in time" and the constant use of "like" as if it were a form of punctuation. "With all due respect" came fourth. The complete list can be found over at the Plain English Campaign headquarters.
The bottom line is these are just ballpark figures so please bear with me as I attempt to clean up my act.


text:
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Christ University Law Entrance Exam commonly known as CULEE 2017 university level exam. It is conducted to offer admission to aspirants in different undergraduate 3 years and 5 years programmes such as BA, LLB (Honours) and BBA, LLB (Honours) at Christ University. Christ University is the first institution in Karnataka to be accredited by National Assessment and Accreditation Council (NAAC) and University Grants Commission (UGC).SLCU imparts law education to the students from various parts of the country and students of Middle East and Mauritius.
Candidates can get the full details of CULEE Exam 2017 such as Application Form, Exam Pattern, Syllabus, Important Dates and much more on this post.
Christ University will be announcing eligibility criteria of CULEE 2017 in November 2016. Candidates seeking admission to BA LLB and BBA LLB programmes through Christ University Law Entrance Examination must fulfill eligibility criteria of CULEE 2017 before filling up exam application form. Check here for CULEE 2017 eligibility conditions including academic qualification and age required for appearing in the law entrance examination.
|Exam Name:
|Christ University Law Entrance Exam
|Conducting Institute:
|Christ University
|Commonly called as:
|Exam Level:
|University Level
|Law Entrance Exam
Online Applications begin
08th December 2016
Last date of application
Third week of April, 2017
Last date of submitting required documents
Third week of April, 2017
Admit Card
Fourth week of April, 2017
Christ University Law Entrance Examination 2017
Last week of April , 2017
Results announcement
First week of May, 2017
- Candidates need to fulfill CULEE 2017 eligibility criteria as required by School of Law, Christ University for admissions to law programmes including BA LLB and BBA LLB. Candidates must have fulfilled minimum academic qualification for applying for the undergraduate law programmes.
- Candidates need to check the eligibility criteria of CULEE 2017 and fill the application form correctly, as if in any case candidate is found ineligible at any stage of admission, their admission shall stand cancelled. Check here more about CULEE 2017 eligibility criteria.
Age Criteria
- Candidates seeking admissions to BA LLB and BBA LLB programmes should not be more than 20 years of age as on July 1, 2017, as per the Christ University guidelines.
|Application Fee
- Application forms will be available online on their official website or from the Admission Office, School of Law, Christ University
- Candidates need to register online for the Christ University Law Entrance Examination in order to fill the application form of CULEE 2017. The CULEE application form can be filled online only, however, the application forms need to be submitted offline, after taking a print out of the filled-in application at the below mentioned address–
The Office of Admissions,
Christ University,
Hosur Road,
Pin – 560029
- Candidates also need to enclose some important documents along with CULEE 2017 application form. In addition to these, candidates are required to remit INR 1000 as an application fee for CULEE 2017 through online or offline payment modes.
|No of Questions
|English Language, Comprehension Skills, Verbal Reasoning
|General Knowledge, Current Affairs
|Quantitative Aptitude, Numerical Ability, Fundamental Mathematical Operations
|Reasoning: Critical, Analytical and Logical
|Data Analysis and Interpretation
Christ University will be issuing admit card for CULEE 2017 a week before the conduct of examination in April. Candidates need to download the CULEE admit card by logging into their registered accounts. Candidates must download at least two copies of Christ University Law examiantion 2017 admit card (hall ticket) as it will also be required during the seat allotment rounds and no admit card will be re-issued once the examination has been conducted. The applicants are required to keep the second copy of CULEE 2017 admit card safe for future correspondences.
Christ University will be declaring CULEE 2017 results in May. The results for Christ University Law Entrance Exam 2017 will be announced in online mode only. Candidates can check here scores and know their qualifying status for Christ University BA LLB 2017 entrance exam. Candidates will be shortlisted for admissions on the basis of marks and rank obtained in the entrance exam.
Christ University will be conducting counselling of CULEE 2017 in offline mode. The university will be conducting Group Discussion (GD) and Personal Interview (PI)- Writing Ability Test (WAT) for shortlisting candidates. A final merit list will be prepared on the basis of GD and PI-WAT for qualified candidates. Check here the complete admission procedure of Christ University Law 2017.
Christ University
Hosur Road, Bangalore-560029
Karnataka, India
Phone: +91 80 40129100/40129600
Email: [email protected]
Website: http://www.christuniversity.in/


text:
Henry Payne by Henry Payne
- January 03, 2013
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My sentiments exactly. They (both parties) tried to put a band aid on a wound that required major surgery. Once more:
Leadership fails on all three levels of government. Politics triumphs over the needs of the people.
The proverbial can is once more kicked down the road.
The world laughs at us or shakes their heads in disgust, take your pick. America's credibility as a financial superpower diminishes further. The world loses confidence in America as a stable place to invest (buy our Treasuries) and looks further at Asia as a place to invest and start business.
Obama says he isn't going to negotiate on the upcoming "debt ceiling." Since credit ratings for American debt is heavily affected, that can only come back to kick us in the butt.
Add to that the arrogant a-holes gave themselves a raise.
Just before this Obama signed an increase in pay for congress judges and some federal employees. :~(.
Captained by "w", piloted through a narrow channel by Cheney, and a crew of Republicans kept stoking the boilers, for decades. At least even Reagan and even GHW knew enough to patch the tax leak, temporarily, and contain spending, even with their "mini-wars" in Grenada and Panama, but "Desert Storm" only started the destruction for real, it took "Jr", to turn it into a mega-storm bigger and more costly than even Katrina.
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Hiway Inn New Delhi
18, Gagan Vihar (main), Near Preet Vihar,Vikas Marg, Kakari Mor Road, East DElhi,, New Delhi, India Show Map
Check in from 12:00 AM / Check out prior to 12:00 AM
Hiway Inn New Delhi: Find photos, reviews, information, amenities and compare prices for 1 stars hotels in New Delhi, India.
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text: This is no doubt a nice fine dining restaurant. That said, it is all around American... music, hostess, ambiance, flavours (though mixed with local ingredients). My friend and I had dessert and coffee that was undeniably delicious. However, if you are looking for Belizean food, experience, conversation, people or feel --- it certainly is not here.


text:
Read about the talk that stole the show at a recent ME/CFS conference in Simon McGrath's two-part blog.
Discuss the article on the Forums.
FDA should give CFS patients access to Ampligen! --Online Petition
Discussion in 'Petitions' started by JohnnyD, Dec 27, 2012.
Page 1 of 3
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text:
It is very unlikely that this work is public domain in the EU, or in any country where the copyright term is life-plus-70 years. However, it is in the public domain in Canada (where IMSLP is hosted) and other countries where the term is life-plus-50 years (such as China, Japan, Korea and many others worldwide). As this work was first published before 1923 or failed to meet notice or renewal requirements to secure statutory copyright with no "restoration" under the GATT amendments, it is very likely to be public domain in the USA as well.
|First Publication
|Genre Categories
|Caprices; For violin, piano; Scores featuring the violin; Scores featuring the piano; For 2 players
Piano Score
*#438604 - 0.51MB, 6 pp. - 0) (- V/V/33 - 8x⇩ - Fmhenry
Violin Part
*#438605 - 0.25MB, 4 pp. - 0) (- V/V/33 - 15x⇩ - Fmhenry
|Work Title
|La Chasse in the Style of Cartier
|Alternative. Title
|Kreisler, Fritz
|First Publication.
|Composer Time PeriodComp. Period
|Early 20th century
|Piece Style
|violin, piano
In February, 1935, Kreisler acknowledged this composition as an original one by him. He formerly attributed it to Cartier. (qtd. from Sibley Project description.)


text:
Experts to Address Chicago Market's Living and Giving
Posted on May 19, 2010 by
CHICAGO-The Chicago Market: Living and Giving, scheduled for July 22-28, has scheduled numerous business and retail seminars and special events.
The Market's highlights include a keynote presentation from Jerry Greenfield, co-founder of Ben & Jerry's Homemade Inc., on July 26, and The Power Lunch Seminar Series, where buyers will learn from leading retail consultants.
Speakers at The Power Lunch Seminar Series include Carmen Iezzi, executive director of the Fair Trade Federation; designer Mick Santiago; and Krissa Rossbund, Traditional Home Magazine.
For more information, visit shopchicagomarket.com.
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Scarce bound album of World War 2 Art created by soldiers stationed at Kessler Field in Mississippi. This book was printed in very limited numbers in 1943 by Captain A.M. Klum through the Higgins Press in New Orleans and Milwaukee and dedicated to the officers and men of Kessler Field. Thumbing through this 68 page volume I am astonished at the quality of work in the multiple mediums that was created during the zenith of WW2. The mural of the Flying Tigers Squadron in the day room is by far my personal favorite and the poster art is exceptional. You would be tough pressed to find another copy.
Condition report: Very Good with two solid boards. Slight separation of ring binding at the top of the front cover.
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CENSORED and DELETED (By NASA) 3D-HD
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Follow Michael Chandler as he takes us back to the place it all started, Mizzou. He discusses being a walk-on for University of Missouri Wrestling, the interesting way he met his wife and where he ha watch video >>
Bellator featherweight Gaston Reyno out of Montevideo, Uruguay. Follow: https://twitter.com/gastonreyno https://twitter.com/thejameskrause watch video >>
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Mike Chandler is fighting on David Rickels on November 6th. Follow: https://instagram.com/mikechandlermma/ watch video >>
Former UFC and now Bellator fighter Phil Davis opens up about transitioning organizations, his teammates leaving Alliance MMA, and more. Subscribe: http://www.youtube.com/subscription_center?add_use watch video >>
Former UFC fighter Reza Madadi will talk about resuming his fighting career after a recent jail sentence. Subscribe: http://www.youtube.com /subscription_center?add_user=mmafightingonsbn Check out watch video >>


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Sources tell the WSJ BlackBerry (BBRY +5.9%) is " aiming to run a fast auction process that could be wrapped up by November." The report led shares to spike just before the close.
Meanwhile, sources tell Bloomberg Microsoft (MSFT -2.2%) is "keeping an eye" on BlackBerry in the wake of the Nokia deal. Given Microsoft's commitment to Windows Phone, any interest in BlackBerry from the software giant could be related to the company's BES/network operations center assets rather than its hardware ops.
The reports come as analysts continue to cut estimates. BlueFin Research says its checks indicate there have been major BB10 production cuts, and that BlackBerry has "amassed significant component and finished goods internal inventories, while many carriers and retailers are not restocking."


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Egyptian cuisine consists of the local culinary traditions of Egypt. Egyptian cuisine makes heavy use of legumes and vegetables, as Egypt's rich Nile Valley and Delta produce large quantities of high-quality crops. Egyptian cuisine's history goes back to Ancient Egypt. Archaeological excavations have found that workers on the Great Pyramids of Giza were paid in bread, beer, and onions, apparently their customary diet as peasants in the Egyptian countryside. Dental analysis of occasional desiccated loaves found in tombs confirm this, in addition to indicating that ancient Egyptian bread was made with flour from emmer wheat. Though beer disappeared as a mainstay of Egyptian life following the Muslim conquest of Egypt in the year 641, onions remain the primary vegetable for flavoring and nutrition in Egyptian food. Beans were also a primary source of protein for the mass of the Egyptian populace, as they remain today.
Contributions by Lockesdonkey, Arab Cowboy, and 220.127.116.11.