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Welcome to SEW-WOT.com...
We are personalised embroidery teamwear and workwear specialists SEW-WOT is based just outside Banbury. We specialise in bespoke workwear, Brisca Formula 1 and Brisca Formula 2 stock car racing teamwear. We have been supplying Rebel Racing teamwear ever since we started the company. We also supply V8 Hotstox teamwear, StoxKart teamwear, Modstox teamwear, Banger teamwear, Ministox teamwear, School Club teamwear and Equestrian teamwear.
As well as providing teamwear we also provide bespoke workwear solutions, we work with Sole Traders, Limited companies and Global retail brands so no matter how large or small your business is we will always work with you to ensure you get your exact requirements met.
We use the latest computerised technology to produce individual designs just for you. We embroider designs onto a wide range of goods from shirts, blouses, jackets, polo shirts, t-shirts, caps, beanies, hoodies, sweatshirts, jumpers, fleeces, hi viz, towels and work overalls. Lots of our customers have great ideas but are worried about coming up ideas that will work across their clothing ranges simply give us an idea and we will work up an entire range of complementing items for you. Remember we don't print garments, we only embroider as the quality and durability is far superior.
If you want to get some ideas why not have a look in our fabulous online shop.
Why not drop us an email with any ideas and we will contact you to discuss design ideas and arrange a quote for you.
We can do company logos, names, numbers, sponsor logos, cars or any combination on a full range of team clothing and you'll definitely get some Teamwear to be seen in....
Remember, if you are a fan or just want a single item that's fine either checkout what we have in our online shop or contact us for details of products we simply can't fit on the site yet!
We work closely with all our customers to ensure you get exactly what you want and that you are very proud to wear you SEW-WOT.com teamwear.
We are very proud of our teamwear here's an example of the Our Wainman Racing Jacket as seen in the BBC series Gears and Tears.
We are pleased to work with the best photographers in Oval Racing and have produced ranges from photos taken by Dave Bastock, Paul Tully and STOXPICS.com.
So for any occasion you're celebrating or if you need to spruce up your party, business or team then contact us via the contact us page to let us know your requirements - we will be happy to provide you with a quote.
A totally individual service means that we can produce designs with no minimum quantity, we specialise in one off's.
all designs are copyright sew-wot.com 2008/09/10/11/12/13/14

subset: commoncrawl


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Sell Seamless Welded Straight And Reducing Tee Pipe Fitting
Posted at: Offers to Sell and Export | Posted on: Tue 31 Jul, 2007 1:31 am |
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Product Group: Sanitary pipe fitting
Product Description
Sanitary Welded Tee
Connection mode: Welded
Specification: 1/2"-6", applied to the stainless steel pipeline system
Material quality: SUS304, SUS316L
Standard: ISO, DIN, IDF, 3A, SMS
Application: Pharmacy, beer, food, dairy, beverage, cosmetic, chemical industry
As a professional manufacturer, we can offer all kinds of the sanitary pipe fittings and can manufacture them according to any drawings. If you are interested in, pls don't hesitate to contact us.
Standard: ISO, DIN, IDF, 3A, SMS
Unit Price/Payment: T/T, L/C
Origin: China
Packing: In carton
Min. Order: Negotiable
Transportation: By Sea
Shanghai Xingu Pipe fittings Co.,Ltd is a professional manufacturer and supplier of pipe fittings who has it's own import&export right.
We are the manufacturer of the stainless steel fittings. It is always in the sanitary grade and food grade, with the polish finishment between 3.2--0.8.
Production: elbows,bend pipes,reducers,caps,tees,crosses,lap joint sub ends ,flanges and so on.
Straight cross, clamped cross, butt weld cross, other type cross.
Standard: 3A, ISO, DIN, SMS
Material: 304, 304L, 316, 316L.
Size: 1" 1-1/2" 2" 2-1/2" 3" 4"
We can develop the certain products accordinglt to your drawings
Company Contact:
Contact Name: Mr. LI CHI
Company Name: Shanghai Xingu Pipe Fittings Co.,Ltd
Tel: +86 21-66313613
Fax: +86 21-56031114
Street Address: NO.1188 Jiangchang
Road, Zhabei District, Shanghai, China
Member name: kikomaya
Member Since: 26 July 2007
Total Leads:
6 kikomaya Import Export Business Leads
Business focus: Pipe Fittings, Elbows, Bend Pipes, Reducers, Caps, Tees, Crosses, Lap Joint Sub Ends, Flanges
Safe Import Export Tips
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Import Export Startup Stories
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Zhejiang Leo Building Material Ltd
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: Hongguang Zhao Of Cangzhou Jinxinlei Pipeline, A Manufacturer And Exporter Of Carbon Steel Pipes From China
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All Praises to Jackie's Miracle
"The very meeting of Emeka was purely the Law of Attraction at its best. It's no surprise the story he tells in Jackie's Miracle is the practical application of how the Law of Attraction can actually be taught and applied to anyone who desires more out of life. I was so captivated by the realness of the characters that I forgot about the science and gained a true understanding inside the story. Be prepared to be touched, moved and inspired!"
—Valerie Waugaman – Co-Founder of FITmission.com;
"SIREN" on NBC's American Gladiators;
Youth Empowerment Leader
"Emeka Iwenofu has created a remarkable book in Jackie's Miracle. The characters are real and you feel an immediate connection with them, experiencing their pain as well as their victories in life. I loved reading about Jackie's journey into positive thought and the impact that it had on her life - the knowledge between these pages is both inspiring and timeless."


text:
LION Magazine
By The International Association of Lions Clubs
Open iTunes to buy and download apps.
LION Magazine is the official publication of Lions Clubs International. From community activities to SightFirst (our worldwide blindness prevention program), disaster relief and environmental awareness, LION presents timely, informative articles reflecting the true international flavor of the world's largest service club organization. LION is published 10 times per year plus one special photo edition, with an appeal as diverse as the members it represents. To view our Privacy Policy, please go to www.lionsclubs.org/privacy.
iPad Screenshots
- Free
- Category: Lifestyle
- Released: Nov 06, 2013
- Version: 3.5.2
- Size: 25.3 MB
- Language: English
- Seller: The International Association of Lions Clubs
- © 2013 Reserved
Compatibility: Requires iOS 5.0 or later. Compatible with iPad.


text:
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JUST REDUCED...BRING OFFERS! This BEAUTIFUL, WATER-FRONT site is just under one (1) acre. Multi-Family (16 unit complex) with 1 commercial structure. Complex needs complete rehab. When fully rented, gross monthly income of $8000+ once units have been remodeled. The property has an existing BEACH AREA, an existing SEA WALL, and a DOCK that can be restored. This is a highly desirable area for long-term rentals close to Hurlburt Field in Okaloosa County near Fort Walton Beach, FL. Property could also be cleared for development at 16 units/acre for Multi-Family or 6 units/acre for Single-Family Residence. Conceptuals available upon request.


text: I need some help from the ladies and the fellas. I have been with my soulmate for a year and a half now. I am Madly in love with him and I feel that he is the same with me. But it wasn't always so great. We broke up..... my bad, let me correct it, he broke up with me, said he needed space and just wanted time. I was heart broken, because there were no signs that he needed space. Any how, he took his space. And I didn't want to be with anyone else ( my heart was still there with him ). So when I was out at a function I met this guy (when I say Perfect Gentleman, that is an understatement.) he was the sweetest and most considerate guy i have ever met. He and his fiance had broken it off 6 months prior. We talked and over the 3 months that me and my boyfriend were apart, we (me and the other guy) kicked it hard. Movies, Dave and Busters, Houston's.... you get the picture. And not once, did he even seem to look at me with lustful eyes. He told me that he enjoyed my company, as I did his. Well my boyfriend and I were still cool at the time, and he had been working on improving his life (he says for us, I believe that). So we got back and he treats me Great! (we have our arguements, but who doesn't??) We've been talking about marriage and the future and all that good stuff. But last Wenseday we ran into a problem. The Other guy. Well right before me and my boyfriend got back together I founds out that the other guy knew him, which made me a bit uncomfortable, because they have the same friends and I didn't want people to start lying and saying there was more going on then there was. Any way, when me and my man got back together I stoped talking to my friend that often cause I didn't know what to say. I told my man the truth eventually, but assured him that me and the other guy were really just friends. He said that he trusted me and that if that is what I say it is then that he can do nothing but go with it. Well my friend and I talked and everything was cool...... he wanted to meet up with me after work so that we could have lunch on Thursday (last thursday, he asked on last Tues.) well I was informing my bf that I wouldn't be straight home after work that day cause I was meeting a friend for lunch, when he asked who and I told him, he TOLD me that NO i could not go. That I would hurt him if I did and that it was inappropriate. The question comes to, am I not allowed to have male friends because of his insecurities? When HE broke up with ME I still didn't get with this guy because I loved him and I love him more now then I did then, and as much as I respect and love him, WHY can't I just have friends? Tell me what you think and I'll tell you what happened last thursday after work


text:
New Truck - 2016 Chevrolet Tahoe 2WD 4dr LTZ in Monroe, Nc
Jet Black, 4-Wheel Antilock Disc Brakes, ABS brakes, Auto-dimming Rear-View mirror, Compass, Driver door bin, Driver vanity mirror, Electronic Stability Control, Emergency communication system, Fog Lamps, Front dual zone A/C, Front Heated Reclining Bucket Seats, Front reading lights, Heated & Cooled Driver & Front Passenger Seats, Heated 2nd Row Seats, Heated door mirrors, Heated front seats, Heated rear seats, Heated steering wheel, Illuminated entry, Lane Keep Assist, Low tire pressure warning, Outside temperature display, Overhead console, Passenger vanity mirror, Power-Adjustable Accelerator & Brake Pedals, Rear reading lights, Remote Keyless Entry, Remote keyless entry, Tachometer, Telescoping steering wheel, Tilt steering wheel, Traction control, Trip computer, and Wheels: 20 x 9 Polished-Aluminum. This superb-looking 2016 Chevrolet Tahoe is the rare family vehicle you have been looking for. This vehicle is loaded with all the comforts of home to make your next vacation enjoyable! - This 2016 Chevrolet Tahoe 4dr 2WD 4dr LTZ features a 5.3L 8 Cylinder 8cyl Flex Fuel engine. It is equipped with a 6 Speed Automatic transmission. The vehicle is Pearl with a Jet Black Leather interior. It is offered with a full factory warranty. - Air Conditioning, Climate Control, Cruise Control, Tinted Windows, Power Steering, Power Windows, Power Door Locks, Power Mirrors, Leather Steering Wheel, Leather Shifter, Power Drivers Seat, Power Passenger Seat, Memory Seat Position, Rear Air Conditioning, Clock, Steering Wheel Radio Controls, Driver Airbag, Passenger Airbag, Side Airbags, Keyless Entry, Security System, ABS Brakes, Traction Control, Dynamic Stability, Rear Defogger, Fog Lights, Intermittent Wipers, AM/FM, CD Player, Aluminum Wheels, Leather Interior Surface, Lane Departure Warning, Lane Keeping Assist, Active Suspension, Engine Immobilizer, Locking/Limited Slip Differential, Tow Hitch, Tires - Front Performance, Tires - Rear Performance, Conventional Spare Tire, Running Boards/Side Steps, HID headlights, Daytime Running Lights, Automatic Headlights, Heated Mirrors, Integrated Turn Signal Mirrors, Power Folding Mirrors, Rain Sensing Wipers, Remote Trunk Release, Power Liftgate, Hands-Free Liftgate, Navigation System, MP3 Player, Bluetooth Connection, Auxiliary Audio Input, Smart Device Integration, Satellite Radio, HD Radio, Telematics, WiFi Hotspot, Power Driver Seat, Bucket Seats, Heated Front Seat(s), Driver Adjustable Lumbar, Passenger Adjustable Lumbar, Heated Rear Seat(s), Cooled Front Seat(s), Cooled Rear Seat(s), Mirror Memory, Rear Bucket Seats, 3rd Row Seat, Pass-Through Rear Seat, Rear Bench Seat, Floor Mats, Adjustable Steering Wheel, Heated Steering Wheel, Remote Engine Start, Universal Garage Door Opener, Adjustable Pedals, Auto-Dimming Rearview Mirror, 4-Wheel Disc Brakes, Brake Assist, Driver Air Bag, Front Head Air Bag, Rear Head Air Bag, Navigation from Telematics, Rear Parking Aid, Back-Up Camera, Cross-Traffic Alert, Blind Spot Monitor, Tire Pressure Monitor 3rd Row Seating, Carpeted Floor Mats, Cup Holders, Daytime Running Lights, Heated Mirrors, HID Headlamps, Navigation System, Parking Sensors, Power Adjustable Pedals, Power Lift Gate, Premium Sound, Rear AC Seats, Remote Trunk Lid, Reverse Camera, Roll Stability Control, Running Boards, Side Curtain Airbags, Tire Pressure Monitor - Sales Tax, Title, License Fee, Registration Fee, $549 Doc Fee, and Finance Charges are additional to the advertised price. Plus any dealer installed accessories. -
- Air Conditioning
- Climate Control
- Cruise Control
- Tinted Windows
- Power Steering
- Power Windows
- Power Door Locks
- Power Mirrors
- Leather Steering Wheel
- Leather Shifter
- Power Drivers Seat
- Power Passenger Seat
- Memory Seat Position
- Rear Air Conditioning
- Clock
- Steering Wheel Radio Controls
- Driver Airbag
- Passenger Airbag
- Side Airbags
- Keyless Entry
- Security System
- Abs Brakes
- Traction Control
- Dynamic Stability
- Rear Defogger
- Fog Lights
- Intermittent Wipers
- Am/fm
- Cd Player
- Aluminum Wheels
- Leather Interior Surface
- Lane Departure Warning
- Lane Keeping Assist
- Active Suspension
- Engine Immobilizer
- Locking/limited Slip Differential
- Tow Hitch
- Tires - Front Performance
- Tires - Rear Performance
- Conventional Spare Tire
- Running Boards/side Steps
- Hid Headlights
- Daytime Running Lights
- Automatic Headlights
- Heated Mirrors
- Integrated Turn Signal Mirrors
- Power Folding Mirrors
- Rain Sensing Wipers
- Remote Trunk Release
- Power Liftgate
- Hands-free Liftgate
- Navigation System
- Mp3 Player
- Bluetooth Connection
- Auxiliary Audio Input
- Smart Device Integration
- Satellite Radio
- Hd Radio
- Telematics
- Wifi Hotspot
- Power Driver Seat
- Bucket Seats
- Heated Front Seat(s)
- Driver Adjustable Lumbar
- Passenger Adjustable Lumbar
- Heated Rear Seat(s)
- Cooled Front Seat(s)
- Cooled Rear Seat(s)
- Mirror Memory
- Rear Bucket Seats
- 3rd Row Seat
- Pass-through Rear Seat
- Rear Bench Seat
- Floor Mats
- Adjustable Steering Wheel
- Heated Steering Wheel
- Remote Engine Start
- Universal Garage Door Opener
- Adjustable Pedals
- Auto-dimming Rearview Mirror
- 4-wheel Disc Brakes
- Brake Assist
- Driver Air Bag
- Front Head Air Bag
- Rear Head Air Bag
- Navigation From Telematics
- Rear Parking Aid
- Back-up Camera
- Cross-traffic Alert
- Blind Spot Monitor
- Tire Pressure Monitor 3rd Row Seating
- Carpeted Floor Mats
- Cup Holders
- Daytime Running Lights
- Heated Mirrors
- Hid Headlamps
- Navigation System
- Parking Sensors
- Power Adjustable Pedals
- Power Lift Gate
- Premium Sound
- Rear Ac Seats
- Remote Trunk Lid
- Reverse Camera
- Roll Stability Control
- Running Boards
- Side Curtain Airbags
- Tire Pressure Monitor
Major Accidents, Lemon History and Odometer Problems
» Get A Free CARFAX Record Check
Call Hendrick Chevrolet Cadillac today at 888-221-9271 for more financing information.
3112 Highway 74 West
Monroe, Nc. 28110
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Price: $33,879
2017 Chevrolet Corvette 2dr Grand Sport Coupe W/3lt
Price: $82,333


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114 Items
Born in the Wasatch Mountains of Utah, home of the lightest powder snow on earth. Kuhl is a product driven clothing company that has passion for the outdoor experience. The Kuhl mountain icon represents this mountain culture.
Regular Price: $70.00
Special Price $47.50
Regular Price: $65.00
Special Price $44.95
Regular Price: $69.00
Special Price $44.95
Regular Price: $45.00
Special Price $29.95


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Does this attraction require above average amounts of physical activity (long walks, climbs, stairs or hikes)?
Is this attraction good for couples?
Is this attraction suitable for adults only?
Is this attraction suitable for all ages?
Is this attraction pet friendly?
Would this be a good sunny day activity?
Would this be a good hot day activity?
Would this be a good cold day activity?
Is this an outdoor attraction or activity?
Is this activity accessible without advanced planning or reservations?
Thanks for helping!
Onyx cave is a little gem right outside of town. It's a bit of an odd road to get there, passing some run down aircraft and eventually driving on a 1 lane road.
I'd say the cave...
read more
Onyx cave is a little gem right outside of town. It's a bit of an odd road to get there, passing some run down aircraft and eventually driving on a 1 lane road.
I'd say the cave was a bit smaller than I expected, but the tour was great. You get a headset that leads you through the history and...
My now Wife and I visited this cave back when we were not married yet (about 1973) it had been 40+ years and we had to do it again nice little cave and well kept
If you are visiting Eureka Springs, you should go see this cave. It is cute and the kids enjoyed it.
Was very interesting and educational. Had some interesting items for sale in the gift shop area. Worth a visit.
It was just a small drive out of town and I think it was less than 10$ a person. The cave entrance is at the back of a little house thatbis used as a gift shop. There is also a free museum but it was closed when we went. There inside of the cave is much larger than I expected...
This is a self guided tour walking tour. You receive head phones,when you start you push a green button and learn about that section. You do that for each section. This can be a fun family activity.
This is a self-guided cave tour with headphones. The sites within the cave are awesome. The staff in the gift shop/entrance are friendly and helpful.
An interesting self guided tour. We were provided headsets that allowed us to go at our own pace and listen to the interesting bits of history about the cavern without the hustle of being in a group. Complete with a nice gift shop for bringing inexpensive gifts home for the kids or friends.
My wife and I had no plans of visiting Onyx but a sign out on the main road advertising for the cave caught our attention and we decided to follow it. This in itself was a bit of an adventure, the road twists and turns and is narrow. Just when you think you are going the wrong way another sign...
If you're looking for something that's long enough to be worth the time but short enough to keep smaller kids interested, this is it! Takes about half an hour to walk through with a self guided tour with headphones.
It is Onyx Cave Park open for December 24 ,2016 or December 25 ,2016? If yes , what would be the operation hours? And what is the price entrance for this attraction in Arkansas? My Family it consist of nine (9) members : one (1) Senior [67 years old],four (4) Kids - 14 years old , 10 years old , 9 years old & 7 years old; and four adults - 49 years , 42 years old , 44 years old & 40 years old.
The price for one 72 yr old and two 61 yr olds. How long is the tour?
How much Is admission
How far is this from Rogers, ar? And what is the cost?
See all questions


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Edward Lear
- Edward Lear in Gozo – 150 years latertimesofmalta.comMalta
- IFranciszka Themerson's Lines and Thoughts on display at Shoreditch's l'étrangère galleryislingtongazette.co.uk
- Naturalist Richard Mabe: Suggestions from outside act on my imagination like a magnet on iron filingsThe GuardianKew Gardens
- Discover Polish avant-garde illustrator and designer Franciszka Themerson in Londoncalvertjournal.com
- Reclaiming our baystimesofmalta.com
- David Attenborough: The owl, the pussycat and the perfect parrotsThe Times
- Andrea Wulf's The Invention of Nature wins the Royal Society's science book prizewired.co.ukNorth America
- Are these NZ's 10 weirdest creatures?NZ Heraldislands
- Meeting Beatrix PotterThe Huffington Post (UK)
- A Smell of Burning: The Story of Epilepsy by Colin GrantThe Times
- The 100 best nonfiction books: No 28 – The Hedgehog and the Fox by Isaiah Berlin (1953)The Guardian
- Why Should We Care About Government Cuts to Playgrounds?The Huffington Post (UK)
- Zetter Townhouse Marylebone brings a country-house vibe to LondonThe Globe and MailPeru
- Weekend Edition: 7 Things To Do in New York's Art World Before June 20observer.comChina
- Engineering the World review – Ove Arup, the man who built modernityThe Guardian
- To a chorus of Mr Blue Sky, my wife was the star of her own showThe Guardian
- A hundred and fifty feet above Hyde Park is a scene of glory and bloodlustNew StatesmanGreece
- GGozo Observer: 33rd edition just published by the Gozo Campusgozonews.comIx-Xewkija
- GDar il-Gvernatur inaugurated as Wirt Ghawdex Headquartersgozonews.com
- Queen opens London Zoo's new Land Of The Lions enclosureShropshire Star
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A Frog He Would A-Wooing Go
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|17th century
|English Folk Song
"A Frog He Would A-Wooing Go" is a popular English folk song that was performed by Rowlf the Dog, with commentary from Sam the Eagle, as the UK Spot on episode 301 of The Muppet Show.
Among the song's many variations is "Froggy Went A Courtin'," a folk song that tells a similar story of a frog's romantic pursuit of a mouse, but has a completely different melody and lyrics.


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Author: Lyndsee Simpson
Skip the Jars and Pouches: How to Make Your Own Baby Food and Save Money
Those little jars of goo add up. Want to save money? Here's how to create your own homemade baby food.
6 Times You Think You're Saving Money… When You're Really Not
Buying a Used Car? This Simple Spreadsheet Will Help You Get the Best Deal
Buying a used car can be tricky. Resources like Kelley Blue Book are helpful, but there's more you can do to save money. Use this free spreadsheet to track prices and identify outliers.
Mercury's in Retrograde! Here's What It Means for Your Money
Be the Coolest Secret Santa: These 12 Awesome Gifts Cost Less than $20
What do you get for the co-worker you've only talked to about the weather and TPS reports? Any of these ideas will make you the coolest Secret Santa in recent memory.
What Do You Think: Is It Worth Paying Kids for Good Report Cards?
How to Use Science to Trick Yourself Into Sticking to Your Budget
Figuring out how to save more money isn't always easy -- especially if your own brain is working against you. Here's how to use science to create good financial habits that'll help you stick to your budget.


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|Property type
|Minimum Stay
This lovingly restored farmhouse Stoffngut in Tweng you enchanted by security with a portion of luxury in the midst of sensational Lungau mountains of Obertauern. Lots of sun and dry in the summer, snowy and sunshine in the winter holiday.
Obertauern and Lungau region
Easy to reach you expect to 1,233 meters unforgettable days in the exceptional Stoffngut in the Lungau region. The ancient property directly by the ski and hiking area, and Obertauern Lungau is both paradise and experience in a retreat. Tasteful details, useful equipment and natural materials give the Stoffngut his irrepressible charm and make it an ideal holiday home.?Ambitious recreation seekers benefit breakfast service offered by such and such a delicious catering at the request of butcher Lankmayr in Mauterndorf in the Lungau region.
The extraordinary farmhouse Stoffngut is full of romance and thrilling history. Warm wood, natural materials make this ancient farmhouse Stoffngut in Obertauern a special piece of jewelry. Ten spacious bedrooms with comfortable beds and healthy sleep mattresses are just the right place in the Lungau region for restful dreams. The modern kitchen in a regal red (one in green) is lavish. Show the useful kitchen appliances, how nice to give the feel of ancient times can be combined with modern comfort. The spacious dining area invites you to a boisterous rounds like romantic evenings and the stove promises in the farmhouse Stoffngut relaxing hours.
Adequate storage and drying room and a ski boot heating ensure a winter holiday in Obertauern ideal conditions for your winter sports equipment.
Sauna u. Spa. in the house


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Second Grade Worksheets and Printables
Featuring vivid illustrations and clear instructions, these second grade worksheets cover a wide range of subjects to supplement your child's education. Topics include addition, subtraction, money, word building, adjectives and story-telling. If your kids need a greater challenge, try our third grade worksheets.
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Second Grade
Practice Reading Vowel Diphthongs: ou
Help your second grader practice decoding words with the vowel combination "ou", as in the words blouse and bounce.
Spell It! For Second Grade, #1
This spelling worksheet gives kids the opportunity to practice spelling common words, such as "meal" and "talk".
Multiplication: Add & Multiply Acorns
These acorns are not just falling ... they're multiplying! Kids will complete the addition and multiplication number sentence represented by the acorn pictures.
Fraction Practice: Color It!
To complete this second grade math worksheet, kids color parts of shapes to show the fractions 1/2, 1/3, and 1/4.
Number Line Word Problems
This word problem worksheet will help your child learn how to solve math problems by measuring values on a number line.
Fraction Math: Find 1/4
Fractions can be confusing, but with a little practice your child can become a fraction pro. Practice with this second grade fractions worksheet.
Cesar Chavez Coloring Page
Get to know an important labor rights activist, Cesar Chavez, with this coloring page, featuring some important facts about him.
Hundreds Chart Puzzle
Get to know your numbers with a number chart jigsaw puzzle! Your child can cut up this chart. and use his knowledge of number patterns to put it back together.
Family Map
Track your ancestors' journey across the U.S. on this fun family mapping sheet.
Capitalization Rules: Sentences
Help your second grader get a handle on the rules of capitalization by correcting the capitalization in a short paragraph.
Butterfly Mandala
Try coloring a unique pattern on the wings of each butterfly in this butterfly mandala.
Multiplying by 3
Practice multiplying by 3 with this sweet, cookie-themed worksheet. Multiplication is a lot more fun when ice cream is involved!
There, Their, They're
There are many ways to learn the difference between "there", "their" and "they're". Help your child determine the difference with this practice sheet!
KenKen® Puzzle: Tiger
This addition and subtraction KenKen® puzzle is a perfect second grade challenge!
Multiplying by 2
Practice multiplying by 2 the easy way: just pretend you're counting by twos!
Insect Body Parts
Bug out on some insect anatomy! Can you name each part of the insect? Try making an educated guess by using what you already know about animal body parts.
All About Transportation: Which Mode?
Choosing your transportation often depends on where you want to go. Can your child pick the best form of transportation for each trip?
Rounding to the Nearest Hundred
Get your lasso ready, it's time to round up the hundreds herd! Your rounding rancher will practice estimating numbers to the nearest hundred.
Antonym Crossword
On this second grade reading worksheet, each crossword puzzle clue is a sentence with a highlighted word. Kids write the antonym of the word in the puzzle.
Present Tense for Kids
Practice present tense for kids! This worksheet asks students to change verbs in given sentences, by spelling the words with -ing at the end.
Camping Worksheet
This camping worksheet is a fun and exciting printable board game. Whoever collects the most camping gear wins the game!


text: F.Y.I. Hardie Grant Media appoints MD January 31, 2013 12:27 1 Hardie Grant Media has appointed Jeff Trounce as managing director. Trounce moves from commercial director to helm the business as founder Fiona Hardie moves to chair of the company. The announcement: Jeff Trounce has been appointed Managing Director of Hardie Grant Media, as long-standing MD and founder, Fiona Hardie, moves to the role of Chair of the company. Jeff Trounce joined Hardie Grant over a year ago as Commercial Director of the broader Hardie Grant business, and now takes the helm of Hardie Grant Media, as it continues its expansion in custom media as well as building its own content assets in print and digital. Trounce was previously global manager for Lonely Planet's commercial division, overseeing custom content development and brand management. Prior to that he was Publishing Director at Text Media in Melbourne. Jeff Trounce said, "I'm really excited to be leading a business that has such a great reputation for quality. Our long-term client relationships are testimony to the results we're able to generate, and our ability to innovate, and I can genuinely say the culture within HGM is terrific – people here are both creative and energetic. I'm not shy at all about declaring what's usually referred to as "an aggressive growth phase to be our aim, and hope to make some announcements about that shortly." Fiona Hardie commented, "This is a great opportunity for HGM to tap Jeff's experience as we continue to push into new sectors and markets." Source: Hardie Grant Media press release topics Hardie Grant Media, print media Share Tweet Share Comments: 1 Good Moron 3 Feb 13 Congrats Jeff, The A team are running Hardie Grant. Reply Comments are closed. ADVERTISEMENT Most Discussed 60 Comments How to Ritson: A beginner's guide to denial, dismissal & downplay 29 Comments CU in the NT 23 Comments Calling bullshit on the internet 101 22 Comments Mark Latham, Rowan Dean and Ross Cameron land Sunday morning 'Outsiders' show on Sky News 20 Comments Aldi's Americans try to take over Christmas in new work by BMF


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Fendi Grey Chinchilla Fur Quarter Length Long Sleeve Coat
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Rare and incredible softest 100% genuine chinchilla fur coat from Fendi couture. Natural grey-white coloring throughout. This coat also features a wide, shallow neckline, long sleeves, and hip pockets. Front hook-and-eye closure and fully lined. Comes with box.
Christian Dior
Christian Dior Black Mesh Rhinestone Beaded Slip On "Dior Fusion" Sneakers
Sz 37
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We found 13 vacation rentals in conversano
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Villa Mimosa
Ref: ItaCon170152 Rooms:1+ (baths) From €28
Villa Mimosa is a holiday home located in Conversano, 27 km from Bari. It provides free private parking. Free WiFi is pr..
La Reggia di Nausicaa
Ref: ItaCon167701 Rooms:1+ (baths) From €30
Located 27 km from Bari, La Reggia di Nausicaa offers accommodation a 15 minute walk from the centre of Conversano. The ..
Porta degli Antichi Casali
Ref: ItaCon170083 Rooms:2+ (baths) From €39
Featuring free WiFi, Porta degli Antichi Casali offers pet friendly accommodation in Conversano. Each room at this bed ..
Dimora San Benedetto
Ref: ItaCon168728 Rooms:1+ (baths) From €45
Located in Conversano in the Apulia Region, this detached holiday home is 27 km from Bari. The air conditioned unit is 2..
Dimora delle Badesse
Ref: ItaCon181308 Rooms:4+ (baths) From €45
Offering fully equipped rooms with traditional stone walls, Dimora delle Badesse is a 5 minute walk from Conversano Medi..
Dimora Lucia e Dalila
Ref: ItaCon159917 Rooms:1+ (baths) From €49
Dimora Lucia e Dalila offers pet friendly accommodation in Conversano, 27 km from Bari. The holiday home comes with air ..
Fiori Di Ciliegio
Ref: ItaCon167006 Rooms:1+ (baths) From €60
Located in Conversano, Fiori Di Ciliegio offers self catering accommodation with free WiFi. The air conditioned unit is ..
Dimora di Charme Montepaolo
Ref: ItaCon173882 Rooms:10+ (baths) From €75
Located 2.5 km from Conversano, Agriturismo Montepaolo is a 16th century stone house with a Puglia restaurant, surrounde..
Montepaolo Dimora di Charme
Ref: ItaCon146272 Rooms:2+ (baths) From €90
In a rural location
Ciao Hotel Residence
Ref: ItaCon259959 Rooms:2+ (baths) From €99
Near Castle of Conversano


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Model: 6300
Manufacturer: BOSCH
Request: I recently purchased a BOSCH clothes dryer after checking it out with a recent review through CONSUMER REPORTS. This dryer is computerized. It has a dial to select what type of clothing is being washed; i.e., cotton, etc. and also has buttons to start and stop it. Does any one out there have this particular model? I am curious to find out how you like it as I have had an old fashioned dryer with minimal selections on it for the last 45 years. Thank you in advance for your help.
joesgirl from Beverly, MA
I dn't mean to be negative, but really read your warranty info on this product - with all it's computerization it could lead to costly repairs if things aren't covered - just like most cars these days everything that is computerized usually carries a high price tag to fix. I really hope this product works well for you - it sounds great, but just be cautious! - Take care!
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Flusol Forte is in over more than 50 years the most reliable glass cleaner in horticulture.Flusol Forte is a greenhouse cleaner based on fluoride and has been specially developed for the removal of soot, rust, dust, etc. Flusol Forte is the best way to clean your greenhouse thoroughly and can be applied on both glass and foil.CharacteristicsThe vapor effect of Flusol Forte may harm neighboring crops. Use Flusol Forte only in an empty greenhouse.ApplicationFlusol Forte is ideal for cleaning heavily soiled surfaces. The product is suitable for glass and foil. Note: glass coatings can be damaged by Flusol Forte. Inquire for use of the possibilities. It is not suitable for the removal of screen materials.
InstructionsApply Flusol Forte in a water-diluted solution evenly on a damp surface. Depending on the contamination, the dosage can be adjusted. Caution: Don't let Flusol Forte dry in on the glass.Spray the dirt after an exposure time of 10-15 minutes, or dry in with a powerful jet and plenty of water.
RegulationsFlusol Forte can only be used in an empty greenhouse;Do not use Flusol Forte in the proximity of lily or freesias;Do not let Flusol Forte dry;Do not use Flusol Forte in combination with other cleaning products.DosageNormal pollution: 6%With regular yearly use it is possible, after testing, that there is a dilution of 3%.Needed per hectare: 2500 L solution.EnvironmentSlops of Flusol Forte may be discharged on surface water in accordance of the Koninklijk Besluit Glastuinbouw dated on 21-02-2002. Clean used equipment and materials after treatment with water.Pack sizesFlusol Forte is available in pack sizes of:Can 20 liters. (28 cans / pallet)


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at Charles W. Schwab where it costs $19.95 for up to 1,000 shares. Why so much? Charles only charges me $12.95 and there is no limit on the number of shares. glh
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Talented music producer DREY BEATZ officially kicks off the yuletide with a grand slam he calls 'BLOOD ON THE DANCE FLOOR' and he enlists the vocal performance of rising singer CEEZA MILLI. 81 more words
Tags » Blood On The Dance Floor
BotDF by funrat24 on polyvore.com
So I was just scrolling through Tumblr and I come across a tour dates list post for Blood on the Dance Floor and usually I keep scrolling past these things (becasue I'm generally broke/can't get to the venue anyway) but something caught my eye. 606 more words
Blood On The Dance Floor - Jayy quit this morning
So I don't usually post on current events or anything that didn't fall out of late 90s or early 2000s but I am actually a bit lost for words here… 390 more words
Shared from WordPress: Fan-produced Documentary Shows Michael Jackson's Continued Impact on the World
A newly released fan-produced documentary about Michael Jackson's fans, known collectively as "Moonwalkers," captures his influence on their lives across time starting from their earliest exposure, to the height of his career, and since his passing. 37 more words
And The Poster Saga Continues
Currently Listening To: "What The Hell" -Avril Lavigne
Heh, starting the year bitching ~ but hey that is what I am good at. ><
To continue the saga of not receiving what I've paid for…. 582 more words


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05-1242 | Anti-trimethyl-Histone H3 (Lys9) Antibody, clone 6F12-H4
Use Anti-trimethyl-Histone H3 (Lys9) Antibody, clone 6F12-H4 (mouse monoclonal antibody) validated in ChIP, PIA, IF, DB, ChIP-seq, WB to detect trimethyl-Histone H3 (Lys9) also known as H3K9me3, Histone H3 (tri methyl K9).
|Replacement Information
Special Offers
Key Specifications Table
|Species Reactivity
|Key Applications
|Antibody Type
|PIA, IF, DB, ChIP-seq, WB, ChIP
|Monoclonal Antibody
|Product Information
|Purified antibody supplied at 1 mg/ml in 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl, 0.05% NaN3.
|Physicochemical Information
|Materials Information
|Toxicological Information
|Safety Information according to GHS
|Safety Information
|Storage and Shipping Information
|Storage Conditions
|Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid freezing, which can damage IgG and affect product performance.
|Packaging Information
|Material Size
|200 µL
|Transport Information
|Supplemental Information
Certificates of Analysis
References | 15 Available | See All References
|Reference overview
|Pub Med ID
|Independent Mechanisms Target SMCHD1 to Trimethylated Histone H3 Lysine 9-Modified Chromatin and the Inactive X Chromosome.
Brideau, NJ; Coker, H; Gendrel, AV; Siebert, CA; Bezstarosti, K; Demmers, J; Poot, RA; Nesterova, TB; Brockdorff, N
Molecular and cellular biology 35 4053-68 2015
Show Abstract
The chromosomal protein SMCHD1 plays an important role in epigenetic silencing at diverse loci, including the inactive X chromosome, imprinted genes, and the facioscapulohumeral muscular dystrophy locus. Although homology with canonical SMC family proteins suggests a role in chromosome organization, the mechanisms underlying SMCHD1 function and target site selection remain poorly understood. Here we show that SMCHD1 forms an active GHKL-ATPase homodimer, contrasting with canonical SMC complexes, which exist as tripartite ring structures. Electron microscopy analysis demonstrates that SMCHD1 homodimers structurally resemble prokaryotic condensins. We further show that the principal mechanism for chromatin loading of SMCHD1 involves an LRIF1-mediated interaction with HP1γ at trimethylated histone H3 lysine 9 (H3K9me3)-modified chromatin sites on the chromosome arms. A parallel pathway accounts for chromatin loading at a minority of sites, notably the inactive X chromosome. Together, our results provide key insights into SMCHD1 function and target site selection.
|miR-142-5p and miR-130a-3p are regulated by IL-4 and IL-13 and control profibrogenic macrophage program.
Su, S; Zhao, Q; He, C; Huang, D; Liu, J; Chen, F; Chen, J; Liao, JY; Cui, X; Zeng, Y; Yao, H; Su, F; Liu, Q; Jiang, S; Song, E
Nature communications 6 8523 2015
Show Abstract
Macrophages play a pivotal role in tissue fibrogenesis, which underlies the pathogenesis of many end-stage chronic inflammatory diseases. MicroRNAs are key regulators of immune cell functions, but their roles in macrophage's fibrogenesis have not been characterized. Here we show that IL-4 and IL-13 induce miR-142-5p and downregulate miR-130a-3p in macrophages; these changes sustain the profibrogenic effect of macrophages. In vitro, miR-142-5p mimic prolongs STAT6 phosphorylation by targeting its negative regulator, SOCS1. Blocking miR-130a relieves its inhibition of PPARγ, which coordinates STAT6 signalling. In vivo, inhibiting miR-142-5p and increasing miR-130a-3p expression with locked nucleic acid-modified oligonucleotides inhibits CCL4-induced liver fibrosis and bleomycin-induced lung fibrosis in mice. Furthermore, macrophages from the tissue samples of patients with liver cirrhosis and idiopathic pulmonary fibrosis display increased miR-142-5p and decreased miR-130a-3p expression. Therefore, miR-142-5p and miR-130a-3p regulate macrophage profibrogenic gene expression in chronic inflammation.
|Alterations of epigenetic signatures in hepatocyte nuclear factor 4α deficient mouse liver determined by improved ChIP-qPCR and (h)MeDIP-qPCR assays.
Zhang, Q; Lei, X; Lu, H
PloS one 9 e84925 2014
Show Abstract
Hepatocyte nuclear factor 4α (HNF4α) is a liver-enriched transcription factor essential for liver development and function. In hepatocytes, HNF4α regulates a large number of genes important for nutrient/xenobiotic metabolism and cell differentiation and proliferation. Currently, little is known about the epigenetic mechanism of gene regulation by HNF4α. In this study, the global and specific alterations at the selected gene loci of representative histone modifications and DNA methylations were investigated in Hnf4a-deficient female mouse livers using the improved MeDIP-, hMeDIP- and ChIP-qPCR assay. Hnf4a deficiency significantly increased hepatic total IPed DNA fragments for histone H3 lysine-4 dimethylation (H3K4me2), H3K4me3, H3K9me2, H3K27me3 and H3K4 acetylation, but not for H3K9me3, 5-methylcytosine,or 5-hydroxymethylcytosine. At specific gene loci, the relative enrichments of histone and DNA modifications were changed to different degree in Hnf4a-deficient mouse liver. Among the epigenetic signatures investigated, changes in H3K4me3 correlated the best with mRNA expression. Additionally, Hnf4a-deficient livers had increased mRNA expression of histone H1.2 and H3.3 as well as epigenetic modifiers Dnmt1, Tet3, Setd7, Kmt2c, Ehmt2, and Ezh2. In conclusion, the present study provides convenient improved (h)MeDIP- and ChIP-qPCR assays for epigenetic study. Hnf4a deficiency in young-adult mouse liver markedly alters histone methylation and acetylation, with fewer effects on DNA methylation and 5-hydroxymethylation. The underlying mechanism may be the induction of epigenetic enzymes responsible for the addition/removal of the epigenetic signatures, and/or the loss of HNF4α per se as a key coordinator for epigenetic modifiers.
|Ligand-dependent corepressor contributes to transcriptional repression by C2H2 zinc-finger transcription factor ZBRK1 through association with KRAB-associated protein-1.
Calderon, MR; Verway, M; Benslama, RO; Birlea, M; Bouttier, M; Dimitrov, V; Mader, S; White, JH
Nucleic acids research 42 7012-27 2014
Show Abstract
We identified a novel interaction between ligand-dependent corepressor (LCoR) and the corepressor KRAB-associated protein-1 (KAP-1). The two form a complex with C2H2 zinc-finger transcription factor ZBRK1 on an intronic binding site in the growth arrest and DNA-damage-inducible α (GADD45A) gene and a novel site in the fibroblast growth factor 2 (FGF2) gene. Chromatin at both sites is enriched for histone methyltransferase SETDB1 and histone 3 lysine 9 trimethylation, a repressive epigenetic mark. Depletion of ZBRK1, KAP-1 or LCoR led to elevated GADD45A and FGF2 expression in malignant and non-malignant breast epithelial cells, and caused apoptotic death. Loss of viability could be rescued by simultaneous knockdowns of FGF2 and transcriptional coregulators or by blocking FGF2 function. FGF2 was not concurrently expressed with any of the transcriptional coregulators in breast malignancies, suggesting an inverse correlation between their expression patterns. We propose that ZBRK1, KAP-1 and LCoR form a transcriptional complex that silences gene expression, in particular FGF2, which maintains breast cell viability. Given the broad expression patterns of both LCoR and KAP-1 during development and in the adult, this complex may have several regulatory functions that extend beyond cell survival, mediated by interactions with ZBRK1 or other C2H2 zinc-finger proteins.
|Suv39h1 mediates AP-2α-dependent inhibition of C/EBPα expression during adipogenesis.
Zhang, ZC; Liu, Y; Li, SF; Guo, L; Zhao, Y; Qian, SW; Wen, B; Tang, QQ; Li, X
Molecular and cellular biology 34 2330-8 2014
Show Abstract
Previous studies have shown that CCAAT/enhancer-binding protein α (C/EBPα) plays a very important role during adipocyte terminal differentiation and that AP-2α (activator protein 2α) acts as a repressor to delay the expression of C/EBPα. However, the mechanisms by which AP-2α prevents the expression of C/EBPα are not fully understood. Here, we present evidence that Suv39h1, a histone H3 lysine 9 (H3K9)-specific trimethyltransferase, and G9a, a euchromatic methyltransferase, both interact with AP-2α and enhance AP-2α-mediated transcriptional repression of C/EBPα. Interestingly, we discovered that G9a mediates dimethylation of H3K9, thus providing the substrate, which is methylated by Suv39h1, to H3K9me3 on the C/EBPα promoter. The expression level of AP-2α was consistent with enrichment of H3K9me2 and H3K9me3 on the C/EBPα promoter in 3T3-L1 preadipocytes. Knockdown of Suv39h markedly increased C/EBPα expression and promoted adipogenesis. Conversely, ectopic expression of Suv39h1 delayed C/EBPα expression and impaired the accumulation of triglyceride, while simultaneous knockdown of AP-2α or G9a partially rescued this process. These findings indicate that Suv39h1 enhances AP-2α-mediated transcriptional repression of C/EBPα in an epigenetic manner and further inhibits adipocyte differentiation.
|Western Blotting
|High levels of glucose induce metabolic memory in cardiomyocyte via epigenetic histone H3 lysine 9 methylation.
Xi-Yong Yu,Yong-Jian Geng,Jia-Liang Liang,Saidan Zhang,He-Ping Lei,Shi-Long Zhong,Qiu-Xiong Lin,Zhi-Xin Shan,Shu-Guang Lin,Yangxin Li
Molecular biology reports 39 2012
Show Abstract
Diabetic patients continue to develop inflammation and cardiovascular complication even after achieving glycemic control, suggesting a metabolic memory. Metabolic memory is a major challenge in the treatment of diabetic complication, and the mechanisms underlying metabolic memory are not clear. Recent studies suggest a link between chromatin histone methylation and metabolic memory. In this study, we tested whether histone 3 lysine-9 tri-methylation (H3K9me3), a key epigenetic chromatin marker, was involved in high glucose (HG)-induced inflammation and metabolic memory. Incubating cardiomyocyte cells in HG resulted in increased levels of inflammatory cytokine IL-6 mRNA when compared with myocytes incubated in normal culture media, whereas mannitol (osmotic control) has no effect. Chromatin immunoprecipitation (ChIP) assays showed that H3K9me3 levels were significantly decreased at the promoters of IL-6. Immunoblotting demonstrated that protein levels of the H3K9me3 methyltransferase, Suv39h1, were also reduced after HG treatment. HG-induced apoptosis, mitochondrial dysfunction and cytochrome-c release were reversible. However, the effects of HG on the expression of IL-6 and the levels of H3K9me3 were irreversible after the removal of HG from the culture. These results suggest that HG-induced sustained inflammatory phenotype and epigenetic histone modification, rather than HG-induced mitochondrial dysfunction and apoptosis, are main mechanisms responsible for metabolic memory. In conclusion, our data demonstrate that HG increases expression of inflammatory cytokine and decreases the levels of histone-3 methylation at the cytokine promoter, and suggest that modulating histone 3 methylation and inflammatory cytokine expression may be a useful strategy to prevent metabolic memory and cardiomyopathy in diabetic patients.
|p53-mediated heterochromatin reorganization regulates its cell fate decisions.
Mungamuri, SK; Benson, EK; Wang, S; Gu, W; Lee, SW; Aaronson, SA
Nature structural & molecular biology 19 478-84, S1 2012
Show Abstract
p53 is a major sensor of cellular stresses, and its activation influences cell fate decisions. We identified SUV39H1, a histone code 'writer' responsible for the histone H3 Lys9 trimethylation (H3K9me3) mark for 'closed' chromatin conformation, as a target of p53 repression. SUV39H1 downregulation was mediated transcriptionally by p21 and post-translationally by MDM2. The H3K9me3 repression mark was found to be associated with promoters of representative p53 target genes and was decreased upon p53 activation. Overexpression of SUV39H1 maintained higher levels of the H3K9me3 mark on these promoters and was associated with decreased p53 promoter occupancy and decreased transcriptional induction in response to p53. Conversely, SUV39H1 pre-silencing decreased H3K9me3 levels on these promoters and enhanced the p53 apoptotic response. These findings uncover a new layer of p53-mediated chromatin regulation through modulation of histone methylation at p53 target promoters.
|Western Blotting
|Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development.
Ma, P; Pan, H; Montgomery, RL; Olson, EN; Schultz, RM
Proceedings of the National Academy of Sciences of the United States of America 109 E481-9 2012
Show Abstract
Dramatic changes in chromatin structure and histone modification occur during oocyte growth, as well as a global cessation of transcription. The role of histone modifications in these processes is poorly understood. We report the effect of conditionally deleting Hdac1 and Hdac2 on oocyte development. Deleting either gene has little or no effect on oocyte development, whereas deleting both genes results in follicle development arrest at the secondary follicle stage. This developmental arrest is accompanied by substantial perturbation of the transcriptome and a global reduction in transcription even though histone acetylation is markedly increased. There is no apparent change in histone repressive marks, but there is a pronounced decrease in histone H3K4 methylation, an activating mark. The decrease in H3K4 methylation is likely a result of increased expression of Kdm5b because RNAi-mediated targeting of Kdm5b in double-mutant oocytes results in an increase in H3K4 methylation. An increase in TRP53 acetylation also occurs in mutant oocytes and may contribute to the observed increased incidence of apoptosis. Taken together, these results suggest seminal roles of acetylation of histone and nonhistone proteins in oocyte development.
|Epigenomics: maternal high-fat diet exposure in utero disrupts peripheral circadian gene expression in nonhuman primates.
Suter, M; Bocock, P; Showalter, L; Hu, M; Shope, C; McKnight, R; Grove, K; Lane, R; Aagaard-Tillery, K
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 25 714-26 2011
Show Abstract
The effect of in utero exposure to a maternal high-fat diet on the peripheral circadian system of the fetus is unknown. Using mRNA copy number analysis, we report that the components of the peripheral circadian machinery are transcribed in the nonhuman primate fetal liver in an intact phase-antiphase fashion and that Npas2, a paralog of the Clock transcription factor, serves as the rate-limiting transcript by virtue of its relative low abundance (10- to 1000-fold lower). We show that exposure to a maternal high-fat diet in utero significantly alters the expression of fetal hepatic Npas2 (up to 7.1-fold, Pless than 0.001) compared with that in control diet-exposed animals and is reversible in fetal offspring from obese dams reversed to a control diet (1.3-fold, Pgreater than 0.05). Although the Npas2 promoter remains largely unmethylated, differential Npas2 promoter occupancy of acetylation of fetal histone H3 at lysine 14 (H3K14ac) occurs in response to maternal high-fat diet exposure compared with control diet-exposed animals. Furthermore, we find that disruption of Npas2 is consistent with high-fat diet exposure in juvenile animals, regardless of in utero diet exposure. In summary, the data suggest that peripheral Npas2 expression is uniquely vulnerable to diet exposure.
|Corepressor protein CDYL functions as a molecular bridge between polycomb repressor complex 2 and repressive chromatin mark trimethylated histone lysine 27.
Zhang, Y; Yang, X; Gui, B; Xie, G; Zhang, D; Shang, Y; Liang, J
The Journal of biological chemistry 286 42414-25 2011
Show Abstract
Polycomb group proteins play essential roles in transcriptional regulation of multiple gene families involved in various pathophysiological processes. It is believed that Polycomb Repressive Complex 2 (PRC2) is targeted to chromatin by the EED subunit to methylate histone H3 lysine 27 (H3K27), leading to a repressive chromatin state that inhibits gene expression. Here we report that the chromodomain-containing protein CDYL specifically recognizes di- and tri-methylated H3K27 (H3K27me2 and H3K27me3) and directly interacts with EZH2, the catalytic subunit of PRC2. We show that CDYL dramatically enhances the methyltransferase activity of PRC2 toward oligonucleosome substrates in vitro. Genome-wide analysis of CDYL targets by ChIP sequencing revealed that CDYL and PRC2 share a number of genomic targets. CDYL is required for chromatin targeting and maximal enzymatic activity of PRC2 at their common target sites. Our experiments indicate that CDYL functions as a molecular bridge between PRC2 and the repressive chromatin mark H3K27me3, forming a positive feedback loop to facilitate the establishment and propagation of H3K27me3 modifications along the chromatin.
|Identification of miRNomes in human liver and hepatocellular carcinoma reveals miR-199a/b-3p as therapeutic target for hepatocellular carcinoma.
Hou, J; Lin, L; Zhou, W; Wang, Z; Ding, G; Dong, Q; Qin, L; Wu, X; Zheng, Y; Yang, Y; Tian, W; Zhang, Q; Wang, C; Zhang, Q; Zhuang, SM; Zheng, L; Liang, A; Tao, W; Cao, X
Cancer cell 19 232-43 2011
Show Abstract
The full scale of human miRNome in specific cell or tissue, especially in cancers, remains to be determined. An in-depth analysis of miRNomes in human normal liver, hepatitis liver, and hepatocellular carcinoma (HCC) was carried out in this study. We found nine miRNAs accounted for ∼88.2% of the miRNome in human liver. The third most highly expressed miR-199a/b-3p is consistently decreased in HCC, and its decrement significantly correlates with poor survival of HCC patients. Moreover, miR-199a/b-3p can target tumor-promoting PAK4 to suppress HCC growth through inhibiting PAK4/Raf/MEK/ERK pathway both in vitro and in vivo. Our study provides miRNomes of human liver and HCC and contributes to better understanding of the important deregulated miRNAs in HCC and liver diseases.
|Cooperative and antagonistic contributions of two heterochromatin proteins to transcriptional regulation of the Drosophila sex determination decision.
Li, H; Rodriguez, J; Yoo, Y; Shareef, MM; Badugu, R; Horabin, JI; Kellum, R
PLoS genetics 7 e1002122 2011
Show Abstract
Eukaryotic nuclei contain regions of differentially staining chromatin (heterochromatin), which remain condensed throughout the cell cycle and are largely transcriptionally silent. RNAi knockdown of the highly conserved heterochromatin protein HP1 in Drosophila was previously shown to preferentially reduce male viability. Here we report a similar phenotype for the telomeric partner of HP1, HOAP, and roles for both proteins in regulating the Drosophila sex determination pathway. Specifically, these proteins regulate the critical decision in this pathway, firing of the establishment promoter of the masterswitch gene, Sex-lethal (Sxl). Female-specific activation of this promoter, Sxl(Pe), is essential to females, as it provides SXL protein to initiate the productive female-specific splicing of later Sxl transcripts, which are transcribed from the maintenance promoter (Sxl(Pm)) in both sexes. HOAP mutants show inappropriate Sxl(Pe) firing in males and the concomitant inappropriate splicing of Sxl(Pm)-derived transcripts, while females show premature firing of Sxl(Pe). HP1 mutants, by contrast, display Sxl(Pm) splicing defects in both sexes. Chromatin immunoprecipitation assays show both proteins are associated with Sxl(Pe) sequences. In embryos from HP1 mutant mothers and Sxl mutant fathers, female viability and RNA polymerase II recruitment to Sxl(Pe) are severely compromised. Our genetic and biochemical assays indicate a repressing activity for HOAP and both activating and repressing roles for HP1 at Sxl(Pe).
|Epigenetic histone H3 lysine 9 methylation in metabolic memory and inflammatory phenotype of vascular smooth muscle cells in diabetes.
Villeneuve, Louisa M, et al.
Proc. Natl. Acad. Sci. U.S.A., 105: 9047-52 (2008) 2008
|Differentially expressed genes are marked by histone 3 lysine 9 trimethylation in human cancer cells.
Wiencke, JK; Zheng, S; Morrison, Z; Yeh, RF
Oncogene 27 2412-21 2008
Show Abstract
Histone H3 lysine 9 trimethylation (H3K9Me3) has been associated with transcriptional repression, but recent findings implicate this chromatin modification in transcriptional activation and mRNA elongation by RNA polymerase II. Here, we applied immunoprecipitation (IP) with a custom DNA tiling microarray containing many transcription factors important in development and cancer (for example homeotic genes; N=683 total genes) to explore the relationship between H3K9Me3 and other histone modifications with the differential expression of genes. Cancer cell lines derived from different tissues (2 leukemia, 2 medulloblastoma) were characterized with IP antibodies to H3K9Me3, H3K4 dimethylation (H3K4Me2) and H3K9 acetylation (H3K9Ac). MV4-11 is known to overexpress the HOXA9 and MEIS1 genes, whereas D283 overexpresses the OTX2 homeobox gene. Gene expression was assessed by Affymetrix U133 array. Mapping the number and size of histone markings demonstrated significant colocalization of H3K9Ac and H3K4Me2 with H3K9Me3, indicating a pattern of putative 'activating' and 'repressive' markings. The median site size was 600-821 bp and 72-95% or 53-80% of chromatin signal sites were located within 1 kb or 500 bp of transcription start sites (TSS), respectively. A relatively small number of genes displayed additional H3K9Me3 sites in the 5'-region distant from the TSS. Comparing genes with modification sites to those without sites in their promoters confirmed the positive associations of H3K9Ac and H3K4Me2 with gene expression and revealed that H3K9Me3 is associated with active genes rather than being a repressive marking as previously thought. The positive regulatory effect of all three types of modifications were quantitatively correlated with site size, and applied to absolute gene expression within a single cell line as well as relative expression among pairs of cell lines. Extended patterns of H3K9Me3 upstream of some genes (for example HOXA9 and OTX2) may result from the action of multiple promoter elements. We found an inverse relationship between promoter DNA hypermethylation and H3K9Me3 in three studied genes (HOXA9, TMS1, RASSF1A). The localization of H3K9Me3 downstream of the TSSs of expressed genes and not within promoter regions of hypermethylated and silenced genes is consistent with the proposed coupling of H3K9Me3 with RNA polymerase II. Our results indicate a need for revising aspects of the histone code involving H3 lysine methylation. Awareness of H3K9Me3 as a mark of gene activity, not repression, is especially important for the classification of human cancer using chromatin and histone profiles.
|Dynamic changes in histone H3 lysine 9 methylations: identification of a mitosis-specific function for dynamic methylation in chromosome congression and segregation.
McManus, Kirk J, et al.
J. Biol. Chem., 281: 8888-97 (2006) 2006


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There are only a few foods that have stood the test of time for their properties for healing and renewing the body – As you already know, I have talked about the power of bone …Read the full story »
The "Body" aspect of the trinity of Mind, Body & Spirit required for healing and reaching your goals
The "Mind" aspect of the mind, body & spirit trinity required for healing and reaching your goals
The "Spirit" aspect of the trinity of Mind, Body & Spirit required for healing and reaching your goals
Vegans have started showing up more and more in numbers – it almost seems that people have found a new religion to use as an excuse for an argument or protest – what is the truth behind all this?
Vegan diet is considered a more conscious diet as you eat non-animal products and are meant to be more conscious of the environment and the animals around you – whilst vegan-ism stems from some geniune intention to make the world, and our body a better place to be in – there are some areas you must consider:
- Vegans say that they don't hurt living things and that is the main reason (at least the few I spoke to) for their diet. This is simply not possible to do if you are alive, you will naturally be hurting and killing life around – even when you take a deep breath, you have just killed billions of life forms in the air.
- New studies have shown plants do have nervous systems, and they can feel pain. So if your a vegan based on the reason you don't want to hurt living things, then this is heavily flawed.
That said – vegan and a vegerterian diet can be very healthy for some people, and often mind clearing too – but this is not to say meat eating is harmful – in fact, beef protein is the only protein that is most biolavailable to the body, and ideal for recovery and rebuilding strenght (Romans knew this, this is why all gladiators were fed pure beef diet, whilst the rest of Rome was almost vegeterian).
This post is not intended to take sides on vegans or meat eaters – the point to make is that each individual body type is different, and reason for the diet should be more than just saving life of nature (whilstit is important to be conscious of what you are doing), because you simply can't do that with a diet alone.
There are anemic patients that I have seen, who badly need to eat beef, and it would save their life – but they try to get their protein from beans and think this is the same (and they never recover!) – all they need is 1-6 months protein diet on beef protein, and this restores their blood count very fast.
There are some people who have eaten a lot of deep fried junk in their lifes, and are more than healthy in blood count levels – they could do with a vegeterian diet – as this diet will clear their mind, prevent foggy thinking, make them more energetic and less aggressive and even detox their system from a lot of junk – this does not mean its a permanent lifestyle – life is constantly changing, and so are your body's nutritional requirements – there is no right way – both are right depending on the time and place.
There is a whole thing argument about what foods are best for hair growth in men and women – one side talks about certain foods being better, and the vegetarian food eaters talk about the …
There are a bunch of studies done on hair follicles, and it is already understood that hair follicles do not actually die the way we used to think they do – but the problem is …
The stomach acid has always been attacked by western medicine – and for good apparent reason – that it starts damaging the body when it leaks into places it should not be going.
There are powerful …
Inflammation has been known for a long time, but still many people do not understand it – so let us first explain what on earth is inflammation, and what does it do to the human …


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At about the time the tanks rolled across the Iraqi border in Gulf War II, one of the negative scenarios raised by the pundit was catastrophic success. What if the Coalition troops became too successful, Iraqi resistance all but collapses and the Coalition spearhead advances so fast that it outruns its supply lines?
Equity investors today are faced with a similar possibility of catastrophic success. As 2014 dawns, most of the macro tail risks that the markets were concerned about a year ago have evaporated - with the exception of China (more on that in a future post). The fiscal cliff has been successfully navigated. Europe appears to have bottomed and started to recover, as per the chart below of European PMIs:
In the U.S., Scott Grannis believes the December ISM report suggests that we could see GDP growth of 4%+ this year:
As the chart above suggests, recent readings of the ISM manufacturing index are consistent with overall GDP growth of at least 4%. More importantly, conditions appear to have been improving somewhat in recent months.
4% growth! A 4% growth rate for the economy would turn the Fed's thinking about the effectiveness of QE and other extraordinary measures to interest rate policy normalization. Indeed, the market dialogue seems to be turning in that direction. As an example, Joe LaVorgna at Deutsche Bank's 2014 forecast (via Business Insider) indicates that economic risks are tilted to the upside:
For Deutsche Bank's Joe LaVorgna, the risks to his forecasts seem to be tilted to the upside. In other words, he thinks it's more likely that the the U.S. economy will turn out better than he expects in 2014.
"Generally, when investors ask us this, they always seem to imply the downside risks," wrote LaVorgna in a note to clients on Tuesday. "However, for us, the risk to growth is to the upside, not the downside. Beyond this, the primary downside risk in 2014, as it is in most years, is geopolitical, meaning we get some sort of negative exogenous shock such as an oil price spike."
A test for the Yellen Fed?
If the U.S. economy were to grow at 4% of more, it would put tremendous pressure on the Fed to normalize interest rates relatively quickly. Already, the yield curve has started to steepen in reaction to the news of tapering of QE purchases. If growth were to approach 4%, then the bond market would react first with further steepening of the yield curve and stock prices could react either by rallying on higher earnings expectations or fall because of rising interest rates. Regardless of market direction, sector leadership would change to cyclical and late cycle deep cyclical inflation-sensitive groups.
Julian Emanuel of UBS pointed out (via Business Insider) that past Fed chairs have faced tests early in their tenure. Though the sample size is small, he highlights the chart below of the SPX to gold ratio as a measure of market anxiety during each of the past four Fed chairs terms. If history is any guide, Janet Yellen is likely to face a test of her leadership in the next six months.
Could interest rate normalization be the first test of Yellen's leadership at the Fed? CNBC recently speculated about this challenge:
The recent string of positive economic news could turn into a headache for Janet Yellen as she prepares to take the reins at the Federal Reserve.With gross domestic product growth above 4 percent and the stock market roaring along-save for Thursday's year-opening sell-off-the new central bank chief could have a harder time justifying the Fed's crisis-era monetary policy.
"With growth (in 2014) to the mid-3s and potentially higher, you're actually adding accommodation to the economy," said Joe LaVorgna, chief U.S. economist at Deutsche Bank. "Yes, you're slowing the pace of the buying, but the balance sheet is still growing. It's staggering to me. I just don't see how they're going to get out in a clean way."
Watching for signs of catastrophic success
Investors can never fully forecast the future, but they have to be prepared for different possibilities. Here are some of what I am watching for under this scenario of 4%+ growth. In particular, I am monitoring signs that indicate inflationary expectations are rising.
As the chart below shows, inflationary expectations are starting to tick up. TIPS are starting to outperform 7-10 year Treasuries. The chart below shows TIP rallied out of a relative downtrend against IEF, the 7-10 year Treasury ETF.
Similarly, metal and mining stocks have also rallied out of a relative downtrend against SPX. This group appears to be forming a sideways consolidation pattern against the general market.
Energy stocks, which is another major sector that is sensitive to inflationary expectations, has not rallied out of a relative downtrend yet. However, it is testing a multi-year relative support zone. With Warren Buffett's recent forays into the energy sector (Suncor (NYSE:SU), ExxonMobil (NYSE:XOM) and Philips 66 (NYSE:PSX)), the outlook for this sector appears to be attractive on a longer term basis.
If these charts of inflationary expectations all start to undergo an uptrend, then the risk of a dramatic rise in volatility in stock prices will rise as expectations transition from slow growth to overheating.
Cam Hui is a portfolio manager at Qwest Investment Fund Management Ltd. ("Qwest"). The opinions and any recommendations expressed in the blog are those of the author and do not reflect the opinions and recommendations of Qwest. Qwest reviews Mr. Hui's blog to ensure it is connected with Mr. Hui's obligation to deal fairly, honestly and in good faith with the blog's readers."
None of the information or opinions expressed in this blog constitutes a solicitation for the purchase or sale of any security or other instrument. Nothing in this blog constitutes investment advice and any recommendations that may be contained herein have not been based upon a consideration of the investment objectives, financial situation or particular needs of any specific recipient. Any purchase or sale activity in any securities or other instrument should be based upon your own analysis and conclusions. Past performance is not indicative of future results. Either Qwest or I may hold or control long or short positions in the securities or instruments mentioned.


text: Awesome Red Alert Tesla Coil Makes Quick Work of Allied SoldiersAdam Frucci11/05/07 10:03amFiled to: ZapGadgetsGamingRed AlertTopTeslatesla coils111EditPromoteShare to KinjaToggle Conversation toolsGo to permalink Command and Conquer: Red Alert was the first PC strategy game that I really got into, and it's still one of my all-time favorites. That's why I can't help but fall in love with this homemade Tesla coil setup made as an homage to the classic game. The picture above isn't Photoshopped at all; it's a single, 17-second exposure taken with a Nikon D70. That's not to say no special effects were used, however. Advertisement Because Tesla coils can't actually shoot long, single bursts of lightning at a target, wires were strung between the coil and its targets to act as a path for the energy to move down. The coil then sent out three-foot bursts along the wires. By using the longer exposure, the bursts all strung together to look like a continuous shock.As for the figures, they're all handmade, from the cutout of the zapped Allied soldier to the Tesla soldier to the engineer. Be sure to check out the project page for a detailed breakdown of how it was completed with plenty of photos. [Project Page via Neatorama]Gear from Kinja DealsSleep Soundly With Hearos XtremeYour Pick For Best External Hard Drive: Western Digital My Passport UltraGet Your Own Personal Coca-Cola Fridge To Put Coca-Cola InReply11 repliesLeave a reply


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United States District Court, Third Circuit
September 23, 2013
VERINT SYSTEMS INC. and VERINT AMERICAS INC., Plaintiff,v.CALLCOPY INC., Defendant.
Gregory M. Sleet, Chief District Judge.
On April 8, 2013, the plaintiffs Verint Systems Inc. ("VSI") and Verint Americas Inc. ("VAI") (collectively, "Verint") initiated this action against the defendant CallCopy, Inc. ("CallCopy") alleging that eight (8) CallCopy products (collectively, the "Accused Products") infringe one or more of fifteen (15) identified patents (collectively, the "patents-in-suit"). (D.I. 1.) On July 25, 2013, Verint filed an amended complaint withdrawing infringement allegations for three (3) of the originally identified patents. (D.I. 21.) Presently before the court is CallCopy's Motion to Transfer to the Southern District of Ohio pursuant to 28 U.S.C. § 1404(a). (D.I. 7.) For the reasons that follow, the court will grant the motion.
VSI is a Delaware corporation with its principal place of business in Melville, New York. (D.I. 1 at ¶ 1.) VAI is a Delaware corporation with its principal place of business in Roswell, Georgia. ( Id. at ¶ 2.) VAI is the owner of the patents-in-suit (D.I. 9 at 2.), and is a wholly owned subsidiary of VSI. (D.I. 1 at ¶ 1.) CallCopy is a Delaware corporation with its principal place of business in Columbus, Ohio. ( Id. at ¶ 3.)
The patents-in-suit are generally related to the analysis, recording, monitoring, transmission, and/or security of electronic communications. ( Id. at ¶ 5.) Verint alleges that CallCopy has infringed and continues to infringe the patents-in-suit by manufacturing, using, selling, offering to sell, installing, distributing, exporting, and/or importing the Accused Products. ( Id. at ¶ 8.) On April 18, 2013, CallCopy filed a motion to transfer venue to the Southern District of Ohio. (D.I. 7.)
Pursuant to 28 U.S.C. § 1404(a), "[f]or the convenience of parties and witnesses, in the interest of justice, a district court may transfer any civil action to any other district or division where it might have been brought or to any district or division to which all parties have consented." 28 U.S.C. § 1404(a). Under this provision, a district court may exercise "broad discretion to determine, on an individualized, case-by-case basis, whether convenience and fairness considerations weigh in favor of transfer." Jumara v. State Farm Ins. Co., 55 F.3d 873, 883 (3d Cir. 1995).
The court undertakes a two-step inquiry in order to resolve a motion to transfer. "The court first asks whether the action could have been brought in the proposed transferee venue and then determines whether transfer to a different forum would best serve the interests of justice and convenience." Smart Audio Techs., L.L.C. v. Apple, Inc., 910 F.Supp.2d 718, 724 (D. Del. 2012). At each step, the defendant has the burden to demonstrate that a transfer is appropriate, Jumara, 55 F.3d at 879-80, and "unless the balance of convenience of the parties is strongly in favor of the defendant, the plaintiffs choice of forum should prevail." Shutte v. Armco Steel Corp., 431 F.2d 22, 25 (3d Cir. 1970).
With regard to the second step of the inquiry, where court considers whether the litigation would more conveniently proceed and the interests of justice be better served by transferring the case, the Third Circuit has instructed district courts to look to the various private and public interests protected by § 1404(a) rather than to any "definitive formula." Jumara, 55 F.3d at 879. The private interests may include:
plaintiffs forum preference as manifested in the original choice; the defendant's preference; whether the claim arose elsewhere; the convenience of the parties as indicated by their relative physical and financial condition; the convenience of the witnesses - but only to the extent that the witnesses may actually be unavailable for trial in one of the fora; and the location of books and records (similarly limited to the extent that the files could not be produced in the alternative forum).
Id. (citations omitted). And the public interests may include:
the enforceability of the judgment: practical considerations that could make the trial easy, expeditious, or inexpensive; the relative administrative difficulty in the two fora resulting from court congestion; the local interest in deciding local controversies at home; the public policies of the fora; and the familiarity of the trial judge with the applicable state law in diversity cases.
Id. at 879-80 (citations omitted).
Consistent with the two-step analysis detailed above, the court will first address the question of whether this action might have been brought in the proposed transferee forum. Verint does not dispute CallCopy's assertion that the present action could have been brought in the Southern District of Ohio. See 28 U.S.C. §§ 1338(a) and 1400(b); (D.I. 9 at 4 n.1.) Since Verint could have brought the present action in the Southern District of Ohio, the court proceeds to the second step of the transfer analysis - weighing of the Jumara factors.
The parties have all chosen legitimate forums in which to pursue the present litigation. Verint has chosen to file suit in a district where it is not physically located; therefore, its forum choice is accorded increased weight in the analysis, but less than paramount consideration. See Linex Techs., Inc. v. Hewlett-Packard Co., No. 11-400-GMS, 2013 U.S. Dist. LEXIS 1924, at *10 (D. Del. Jan. 7, 2013) (citing In re Link_A_Media Devices Corp., 662 F.3d 1221');"> 662 F.3d 1221, 1223 (Fed. Cir. 2011)). CallCopy's choice of its principal place of business is also a traditional and legitimate venue. However, plaintiffs have historically been accorded the privilege of choosing their preferred venue for pursuing their claims, the court therefore declines to elevate CallCopy's choice of venue over that of Verint. As such, this factor weighs against transfer.
"[A]s a matter of law, a claim for patent infringement arises wherever someone has committed acts of infringement, to wit, makes uses offers to sell, or sells any patented invention' without authority." Cellectis S.A. v. Precision Biosciences, Inc., 858 F.Supp.2d 376, 381 (D. Del. 2012) (quoting 35 U.S.C. §271(a)). This factor is generally neutral where the defendant in a patent infringement action operates on a national level as the infringement can be viewed as occurring in all districts. Smart Audio, 910 F.Supp.2d at 730. But the court has acknowledged that infringement claims have even deeper roots in the forum where the accused products were developed. Id. Here, CallCopy argues that it has never had a Delaware customer, and that the Accused Products have not been sold in Delaware, are marketed solely in and from Columbus Ohio, and were developed in Columbus, Ohio. (D.I. 8 at 1, 5.) Verint argues that CallCopy solicits sales in Delaware via its website (D.I. 9 at 9-10), and maintains sales positions that service the greater Philadelphia area, Baltimore, and New York City. ( Id. at 3-4.) This factor slightly favors transfer.
In evaluating the convenience of the parties, the court considers: "(l) the parties' physical location; (2) the associated logistical and operational costs to the parties' employees in traveling to Delaware (as opposed to the proposed transferee district) for litigation purposes; and (3) the relative ability of each party to bear these costs in light of its size and financial wherewithal." Smart Audio, 910 F.Supp.2d at 731 (citation omitted). Here, the patent owner VAI is located in Georgia, the parent company VSI is located in New York, and CallCopy is located in Ohio. As such, all parties will need to travel for trial if the case remains in Delaware. Verint argues that New York "is a mere 90 minute train ride from the Delaware courthouse, " (D.I. 9 at 1), but fails to account for the fact that its "employees in Georgia and in the United Kingdom who have had a substantial role in the conception and development of Verint's intellectual property, " ( Id. at 3), are not conveniently located in New York. In cop.trast, transfer would substantially reduce CallCopy's traveling costs. Additionally, Verint is a-larger entity ($796 million corporation with 3, 200 employees) in comparison to CallCopy ($20 million corporation with 134 employees). (D.I. 16 at 7.) These facts favor transfer. But CallCopy is a Delaware corporation, and the court has recognized that an entity's "decision to incorporate in Delaware suggest[s] that the inconvenience of litigating here is somewhat less than the court would ordinarily presume it to be." Linex Techs., 2013 U.S. Dist. LEXIS 1924, at *15. Accordingly, the court finds that this factor is neutral.
Considering the convenience of the witnesses and specifically whether witnesses "actually may be unavailable for trial in one of the fora, " CallCopy has not indicated any third-party witnesses that would be unavailable for trial. Similarly, Verint has not identified any particular third-party trial witnesses, but it "anticipates calling third-party witnesses located within the compulsory process of this court to help prove its infringement claims." (D.I. 9 at 13.) Verint states that it requested a list of CallCopy's customers that operate within the regions of this court's subpoena power, but that CallCopy refused to produce the list. ( Id.) Verint has requested limited venue discovery to reveal Delaware entities that have purchased and/or used the Accused Products and would be outside the subpoena power of a court in the Southern District of Ohio. ( Id. at 14.) The court declines Verint's request for venue discovery as the caselaw it cites in support relates to allowing discovery to establish personal jurisdiction over a defendant, which is not at issue in the present case. As such, the court finds this factor is neutral.
For location of books and records factor, the court has acknowledged that "[i]n patent infringement cases, the bulk of the relevant evidence usually comes from the accused infringer. Consequently, the place where the defendant's documents are kept weighs in favor of transfer to that location." Smart Audio, 910 F.Supp.2d at 732 (citation omitted). And although technological advances have made transportation of electronic evidence far less onerous, the court "cannot simply ignore the location of the relevant books and records." Id. (citing In re Link_A_Media Devices Corp., 662 F.3d 122, 1224 (Fed. Cir. 2011)). As all the documents relating to the design and sale of the Accused Products are located in Columbus, Ohio, (D.I. 8 at 1), this factor weighs slightly in favor of transfer.
With regard to the public interest factors, practical considerations weigh toward transfer and the remaining factors are neutral. For practical considerations, neither party identifies issues of judicial economy and efficiency; rather, they focus their arguments on considerations of private costs and party convenience. Verint notes that transfer would simply shift the expense of local counsel from one party to another. (D.I. 9 at 16.) CallCopy reiterates its convenience argument discussed, above, (DI. 8 at 9), and argues that litigation in the Southern District of Ohio would be more practical because "the majority of trial witnesses will undoubtedly be employees of either CallCopy or [VAI] - for whom Ohio is a more convenient forum." (D.1. 16 at 9.) Further, CallCopy argues that none of the parties have facilities, offices, or employees in Delaware. ( Id. at 1.) Those facts, coupled with the facts detailed in the private interest factor "convenience of the parties" discussion above lead the court to conclude practical considerations of efficiency, expense, and ease favor transfer. Specifically, while the court found the private factor neutral because CallCopy is incorporated in Delaware and, therefore, cannot argue that it is an inconvenient forum, the underlying facts discussed in that section do recommend transfer as the court finds that the aggregate litigation costs would be reduced by litigating in Ohio. Thus, the court concludes that the "practical considerations" factor weighs in favor of transfer.
For relative administrative difficulty, the parties site multiple court statistics.[1] The court finds the factor neutral and notes that this case will be scheduled consistent with the parties' proposal. The local interest factor is neutral because a patent infringement action is more properly described as a national controversy. See Smart Audio, 910 F.Supp.2d at 733-34 (citing Helicos Biosciences Corp. v. Illumina, Inc., 858 F.Supp.2d 367, 375 (D. Del. 2012)). Similarly, enforceability of the judgment, public policies of the fora, and familiarity of the judge with state law are neutral in this transfer analysis.
Considering the Jumara factors as a whole, the court believes that CallCopy has met its burden of demonstrating that the interests of justice and convenience strongly favor transfer. Notably, only Verint's forum preference weighs against transfer, and that preference was not afforded maximum deference in this case. On the other hand, several factors counsel transfer: the location where the claim arose; the location of relevant books and records; and practical considerations that might make trial easier and less expensive.
For the reasons stated above, the court will grant CallCopy's Motion to Transfer to the Southern District of Ohio (D.I. 7) pursuant to 28 U.S.C. § 1404(a).
At Wilmington, this 23rd day of September, 2013, consistent with the Memorandum Opinion issued this same date, IT IS HEREBY ORDERED THAT:
1. The CallCopy Inc.'s Motion to Transfer to the Southern District of Ohio (D.I. 7) is GRANTED; and
2. The above-captioned action is transferred to the U.S. District Court for the Southern District of Ohio.
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The media is always looking for local interest stories. How would you like to see your favorite tree in the local paper's headlines? How about a TV report on your school tree planting? The media does not know your story exists until you tell them.
Areas of Interest: Networking, Technology, Writing
Ingredients: Fax machine, camera, telephone, computer
Take a photograph of your favorite tree – Outline the full story of the tree: how it got there, what type it is, how old it is, where people can find it and why you find it so appealing. Contact the editor of the Home and Garden Section of your local papers. Learn their submission requirements and be persistent.
Write a letter to the editor of your local paper appreciating the urban forest — Share your thoughts and your concerns in an editorial piece. Encourage your fellow community members to take action with you. Express the thoughts you want your elected officials to know. If you are knowledgeable about tree care, share some helpful or timely technical information.
Inform the press of your urban forest activities — Prepare press releases before your plantings, tree tours, community garden creations, Arbor Day or National NeighborWoods Day celebrations. Include the date, time, and location of your event. Give the story a creative title and write one or two short paragraphs describing what journalists can expect. Put the name and phone number of a project contact in the upper left-hand corner and mail or email it to all local TV stations and newspapers. Give each station or paper a reminder call a couple of days before the event.
Develop a relationship with the media — Find out which writers or assignment editors have particular interest in local trees and community action. Be sure to thank those members of the media who do respond and keep them informed of your activities.