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Detection and genotyping of Toxoplasma gondii by real-time PCR and pyrosequencing
Abstract number: s16
Edvinsson B., Lappalainen M., Evengård B., for the European Study Group on Toxoplasmosis
Toxoplasma gondii is a protozoan parasite with worldwide distribution. Primary infections in humans are usually asymptomatic or characterized by non-specific symptoms. The infection is, however, severe or fatal in patients with a suppressed immune system, and is here most often caused by chronic infections that become reactivated. Rapid and sensitive diagnosis by PCR may be of importance for the survival of these patients. PCR techniques used to detect the parasite in patient samples are not yet standardized. We have compared the performance of two real-time PCR assays targeting two different repetitive DNA elements of T. gondii. We also aimed to develop a pyrosequencing assay to discriminate between the three main genotypes of T. gondii; i.e. type I, II and III.
The sensitivities for detecting T. gondii by real-time PCR when targeting the 35-fold repeated B1 gene or a 200300 fold repeated 529 bp elements were compared. The impact on the diagnostic sensitivity when using two different types of internal amplification controls, either amplified by the primers used for detection of T. gondii or by a different set of primers, was tested. For genotyping, real-time PCR and pyrosequencing were used to analyse a fragment of the GRA6 gene in 21 T. gondii isolates.
The sensitivity was higher when the 200300 fold repeated 529 bp element was used. The diagnostic performance of the real-time PCR targeting the 529 bp elements was not affected by inclusion of an internal amplification control. The Pyrosequencing assay discriminated between type I, II and III T. gondii.
Real-time PCR, including an internal amplification control, can be used to detect low amounts of T. gondii in a sample by amplification of a part of the 529 bp elements. This may be of importance if the aim is a sensitive and rapid diagnosis of toxoplasmosis in immunocompromised patients. Pyrosequencing can be used for rapid and high throughput genotyping of the parasite in clinical findings, and should help to further elucidate if there are differences in the clinical manifestations of toxoplasmosis caused by type I, II or III T. gondii.
Session Details
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|XXIst ISTH Congress
|Oxford, UK
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Somewhat of a new era is beginning at Villanova University this season with the Wildcats being a part of the revamped Big East Conference.
Most of the rivals are the same for the Wildcats, but they did lose rivalries with Syracuse and Pittsburgh, while gaining contests against Creighton, Xavier and Butler.
On the personnel side, the Wildcats have plenty of exciting guards, but they have plenty of concerns about their frontcourt play.
With plenty of goings-on at Villanova for the 2013-14 season, here is a look at the five biggest storylines surrounding the Wildcats.

subset: commoncrawl


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The Diving BRATS Club
Hello to all the diving BRATS out there...
BRATS North will be on day trips on the R/V Garloo as often as we can. We have also started Thirsty Thursday diving. Will keep everyone posted as to where and when by the BRATS email list.
Please check our Trip Sign Up Schedule.
Looking forward to seeing you on one of our dive adventures.


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Roadside parking reduction to help semi trucks
- Written by NATE SMITH
Motorists looking to park in or around downtown West Liberty will be afforded two fewer parking spots as village council looks to ease the congestion and traffic flow, especially for tractor-trailers negotiating the intersection at U.S. Route 68 and State Route 245.
Two parking spaces on the north side of State Route 245 adjacent to 101 N. Detroit St., West Liberty, (above) will be eliminated effective April 1 to make more room for eastbound semi traffic. Crews from the Ohio Department of Transportation will move the median line over about seven feet later this year. (EXAMINER PHOTO | NATE SMITH)
Effective April 1, two parking spaces along eastbound S.R. 245 on the north side of the road will be eliminated so that the median line may be moved over about seven feet, making more room for semis to turn onto S.R. 245.
West Liberty Village Council have approved the ordinance on second reading only, and the measure will receive another vote at council's next meeting.
That intersection is frequently the site of fender-bender accidents, village clerk Cindee Boyd said, as drivers facing westbound on S.R. 245 will sometimes attempt to back up and/or move over for wide-turning, 18-wheelers heading east.
Parked cars trying to exit one of those two spots nearest the intersection adjacent to 101 N. Detroit St. only complicate matters further, Ms. Boyd said, adding the Ohio Department of Transportation conducted its own study to determine that eliminating the parking spaces would reduce accidents in that area.
ODOT will move the median line over later this spring, Ms. Boyd said.


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Your attempted working is incredibly vague but I shall take a shot at this.
First of all, judging by your equation, I believe you are using volumes of solids of revolution using circular discs, which uses the formula for revolutions about the y axis.
However, your equation is incorrect if you are to integrate between y = 0 to y = 5.
This is because you turned the cup up-side down with that equation. Integrating between y = 0 and 5 gives you the empty volume of the glass, not the volume of water.
To find the volume of water, either integrate between y = 5 to 10 with your equation, or flip the cup so it's base of 3 cm lies on the x axis. The equation for this is


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Late-nite rambling…
The trade deadline was good to me this year. The Mets sent Xavier Nady to the Pirates for Roberto Hernandez and Oliver Perez. Hernandez throws lots of balls into the crowd. Nady doesn't. As for Perez, I'm hoping I can get a ball from him so I can add his name him to the mini-collection of Perezes on my list: Carlos, Eddie, Melido, Timo, and Yorkis. Right now, "Jones" and "Johnson" are leading "Perez" and a few other names by a slim margin of 6-5. Forget the AL Central; if Oliver comes through for me in the next few weeks, THIS will be the three-way race that has everyone talking–about what a geek I am…speaking of which…that was the insult thrown my way in a recent article on Boston.com by some goofball who couldn't even manage to spell my name right. People, It's Z-A-C-K. See that last letter? It's a kay, not an aitch. Why is this so difficult? It says "Zack" right at the top of this blog, and on my web site, and in my email address. I'm amazed at the number of people who write "Dear Zach…" to me at firstname.lastname@example.com. Enough about that. I'm happy with the Yankees' trade, too, because it'll give me a chance to get a ball from Bobby Abreu today. Yes, today. I'd been thinking about going to Baltimore, but that trip fell through, so I'm going to Yankee Stadium instead. I know there are gonna be 50,000 people, and I know I'll have to sit through TWO national anthems, and I know I said I was done with that place for the year, and I know I'll be putting my streak (442 consecutive games with at least one ball) in jeopardy, but I suddenly got the urge to go, for two reasons. First, I decided that I want to catch my 2,900th ball in San Diego. It's not a major milestone, but it'd still be cool to reach it at a new stadium…and since there aren't too many games left before my trip (August 12-18), I kind of need to take advantage of tonight's game and pad my total. Let's do a little math, shall we? Currently, I'm 33 balls away from 2,900. I'm planning to go to three games at Petco, which seems like a pretty good place to snag, but it IS the summer, and the Padres ARE in first place, and Barry Bonds WILL be there, so the stadium could be packed. Therefore, I can't assume that I'll get an obscene amount of balls. Let's say I walk away with 20 in three games. That still leaves me 13 short. The only other games between now and then that I'd even consider attending (other than tonight's) are the only two night games next week at Shea. Will I even go to both of those games? I don't know. Shea is awful when there are more than 30,000 people, and lately, the crowds have all been around 45,000. I'd feel lucky to get even five balls per game there. Hopefully I can get at least three tonight in the Bronx and then average five per game next week at Shea and then average almost seven per game in San Diego. Sounds reasonable, no? My other reason for wanting to go to Yankee Stadium is that I want to take dozens–if not hundreds–of photos of the place. (Don't forget, the new Yankee Stadium is only a few years away.) Back in high school, I devoted part of a game to roaming and photographing, and I ended up with a bunch of artsy shots…but I've never roamed there with my digital camera, or when I've had the backdrop of a full stadium. And anyway, there are some places in Yankee Stadium where I've NEVER been, like way up in the corners of the upper deck beyond the foul poles. I want to see what that view looks like. To hell with chasing foul balls. All the good seats will be taken anyway. I just want to wander and not worry about the game and inspect the place from all angles. I'm going to photograph the ramps, concourses, concession stands, water fountains…everything…maybe even a bathroom or two, if no one's around. The bathrooms there are awful. Totally cramped.
30 Comments
Nice PR, dude.
I'd like to see a pic from your shoot tonight that says: 1920s. Something that almost makes you see the Bambino, Iron Horse and three-piece business suits.
What up Z?
Man, I don't give many updates now a days. I have been to a few games lately, but I have started to focus on getting autographs instead of balls. I've gotten one ball at each of the last 2 games. I used the simple 'ask them by name' method, and I got the hook up. Now, you don't ask Alex Rodriguez, you ask the Yankees BULLPEN CATCHER in Spanish. I did it right in front of all these loud mouths from New York, too. I had on a generic baseball shirt, and I simply was the 1st to ask.
BTW, people act like the Yank-mees are gods. I was at Ameriquest 2 hours before game time, and zillions of people waited fruitlessly for autographs from their hallowed Bombers. What the h ell ever man.
By contrast, the Royals were in town at my last game. NO BP, they had a stupid father/son or daughter game on the field. I'm probably going to h ell for complaining about that. Well, there was tumbleweed blowing by the Royals dugout. I got 3 sigs and a ball from the greatness of Joe Nelson. What a generic ballplayer this guy is, but I tell you what, he is a very nice guy. I swear he was by the dugout OFFERING to sign autographs for people. Most guys walk by and you stop them, but Joe was almost stopping and offering. Pretty cool guy, I swear I would be like that if I had ever made the SHOW.
OK well, peace out brotha man, and to all the blog readers!
Hey ZacK, at least it's advertisement… I'm sure this guy has some hobby that others think is silly as well… we all have ours.
I know that most of ZacK's readers know this, but this is for the random yellow web journalist who ducks into this blog and reads three lines before closing the window and assuming 95% of what ZacK does. He doesn't take balls from little kids. He doesn't come "swooping in" when balls are thrown to other people, especially kids. I've seen it.
As for the "geek" comment, it amazes me that "journalists" think it's okay to start a flame war just because a column is online. Long lost is the art of research. God forbid one of them actually get in touch with ZacK and go to a game with him, see how he prepares, studies, and calculates beforehand, and watch how polite he is to the players in comparison to the high-pitched screeching of "Over heeeere! Hey! Over heeeeeeeere!" that comes from most fans. Zack can judge a ball from 400 feet away and maneuver around seats, stairs, railings, and clueless people in order to snag a ball. That is skill. Geek or not, the man has skill. It is time these flame-throwers step out from behind their keyboards. ZacK is good for baseball. Period.
Oh yeah, good PR anyway (haha)
Hey Zack. Did you see the catch a fan made last night at last night's Orioles game at Camden Yards? A foul ball was ripped down the right field line and curved into foul territory. It was coming down about 10 to 15 rows back and some guy (who brought his glove) hustled about two rows over, getting around people, in between rows, and reached out and made a nice catch. It was "Top Play #5" (or maybe it was #4) on "Cold Pizza" this morning.
Good luck at Yankee stadium and hope you get a lotta balls from the Jays and hope the Jays rock the Yanks.
Hey who are the worst and best autograph signers on Team USA BASKETBALL?
All I know is that Chris Bosh is pretty nice.
Despite the diss and the misspelling and my late-nite rant, I do need to admit that it's good PR. As for tonight, I'll see what I can do…
Good to hear from you again. I've been wondering what you were up to. You're right…sometimes it can be really easy to get a ball if you just use basic strategies: simple clothing and a polite request. That's cool about Joe Nelson. From what I've read, it sems like Pat Neshek acts the same way. Be well. Hope you get to check back in soon. And keep in mind that "****" no longer gets bleeped. Woo-hoo!
Good point. I appreciate it.
You're the best.
Dammit. No. I missed it. But that's a pretty nice description that you gave.
Thanks. I'll secretly be rooting for the Jays as well, but not when Jeter or A-Rod or Bernie are at bat…and not if Mariano comes in to pitch.
Man, what's up with my friend George? He told me on the phone that most basketball players are jerks, but that when they gather for a Team USA event, they suddenly become much nicer.
Ah, that's too bad. It was a nice catch, nothing spectacular. It's not like he dove over seats and ripped up his face on the concrete or anything, and there wasn't anybody around him to interfere, but it was a nice catch. Too bad you missed it. Oh well. I'm sure it would have been a routine play for you.
ZAck-Nice post.Jimmy The jays got my support as I hate the yankees.YANKSFAN-look for the jays to take the yankees down.
hey zack, i hate to be the bearer of bad news, but olly perez isnt gonna be on the mets roster, hes probably gonna finish the year out in AAA or unless a starter gets injured or maine or pelfrey starts messing up and gets sent down.
Oliver Perez was not the point of the trade between the Mets and Pirates. The only reason the trade was made was for the Mets to make up for the loss of Duaner Sanchez. He won't be up in the Majors until September call-ups.
By the way, did anybody notice that everybody involved in the trade (including Duaner Sanchez) has a "Z" in his name? Very odd. Just an observation.
Sorry, I meant an "X" or a "Z".
Lets see what Burnett can do for the jays the sox pitcher is just giving up run after run!
Thanks for going through all the trouble. I have recieved only two autographs in person, Kobe Bryant (at a Dodger game) and Hall of Famer Magic Johnson (at the mall).
Why is everybody conceding the division to the yankees yeah the red sox lost TROT NIXON AND Jason Varitek to injuries but to concede the division that is giving up a little to early on the sox huh?
Wow this ump is killing us. Keeps giving calls to the Yanks, looks like they paid off the umps 2, dam yanks. Still close game, dam as soon as i say its close its not.
Kaylee, stop talking about the *******' Jays and Red Sox. Nobody cares. You're just making it harder to find the comments I actually want to read here. It's really annoying.
OKay GREG-Will it make you happy if I stop talking about the jays and red sox?!
I'm gonna say Zack is gonna get 7 or 8 balls from tonights game. Maybe more cause most of the Jays are nice so we'll see.
Kaylee how many balls did u get tonight?
Jimmy-I got 4 tonight!
You think it would've been routine for me? Thanks. I'd have to see it before making that statement, though. 'X' and 'Z' are good letters.
Thank you, too. How have you gotten all the balls?
Why'd you have to ruin my day?
You're welcome. Those are some imPRESSive autographs.
Update soon cause I love it when you go to Jays games cause its something I know and I always like learning something new about my favourite Jays players.
my red sox didn't counter the yankees trade for abreu, which they might regret come postseason
Ramones-I know especially now that varitek is hurt that hurt the red sox!(sorry greg after this comments I will shut up about the red sox)I have been warned not to talk about them twice by greg!
Zack-I assume you mean whoo threw them to me.Doug mirabelli gave me one.One came from some indians player It was their 3rd Baseman i think it was Luna.Then i got another on from Gabe kapler During Bp.And The other one came from another indians player…..I think it was Blake.
6am…is that soon enough? Ohmygod I'm tired.
The Red Sox are in touble.
Any interesting conversations with the players? What sections? What happened? Lots of competition? Any good stories?
Not yeasterday didnt get there in time But……..I did get the 4 balls.More interesting stuff today will share later…Got to pay attention to the game now!


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Japanese hunter-gatherers living during the end of the ice age may have turned to pottery as a source of cooking, researchers report in the journal Nature. The study details an analysis of charred material from various ceramic vessels and fragments that revealed some of the earliest evidence of cooking using pottery. Scientists also say they found traces of fatty acids belonging to creatures such as fish and other marine animals. The resulting substance would have only resulted after prolonged exposure to heat -- suggesting long periods of cooking time.
Full Story:ScienceMag.org


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Volunteers are essential to every aspect of our mission – from our hard-working board to those who help with being a greeter. Without volunteers we can't meet our goals of providing access to locally grown food choices and furthering the economic development of our community.
The North Plains Farmers' Market supports and encourages many types of volunteers in its Saturday Market. The market values the time and effort each of you put into making it a success. If you are interested in volunteering, there are many different ways to help.
* How about ringing the cow bell to start the market?
* Do you want to help set up booths?
* Be the Music Coordinator?
* Act as a Greeter or People Counter?
* How about helping with tear down and clean up?
The list goes on….
Read the Volunteer Application Form to find out which position you may enjoy.
If you have made the decision to volunteer at the North Plains Farmers' Market and have submitted your Volunteer Application, we now ask that you take a few extra moments to fill out this Volunteer Release Form.
Thank you for your support and the generous donation of your time and energy.to our market.


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He is also a licensed real estate broker and has written a book on real estate investing, "From Paint Rags to Riches: Becoming a Real Estate Millionaire".
In March of 2007, he began an online radio show called Intelligence Based Living. The shows provide his insights and analysis. He also conducts interviews. The shows are archived and can be heard at www.blogtalkradio.com/DrAffolter.
Dr. Affolter and his wife, Mandy have been married since 1978 and have four grown children. They live in Bellingham, Washington, where he still maintains a chiropractic practice.
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Sayre, Cindy MN, ARNP; Wyant, Sheri MSN, RN, OCN; Karvonen, Colleen MN, RN, CMSRN, CWON
The purpose of this study was to describe the effects of participation in a medical-surgical certification and practice review course on the participants' clinical nursing practice. A descriptive survey was mailed to 119 nurses 4 months after the completion of the 2005 course. The majority responding agreed that participation in the course had a positive effect on their self-confidence, competence, leadership, and initiative. The written clinical examples demonstrated the efficacy of the course in the domains of knowledge acquisition and validation, enhancement of confidence, and the ability to contribute to collegial discussions. These results demonstrate successful achievement of the ultimate goal of staff education: to see nurses translate newly acquired knowledge into practice.
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The Miami Dolphins are in the midst of an active free-agent signing period and as each new Dolphin is signed, their 2014 starting lineup emerges more and more.
Is it complete? Not at all, the Dolphins still have holes to fill but now those holes will have to be filled either through bargain free agents or the draft. What they do now have is a foundation to build upon, making the job easier than it was just nine days ago.
Here's a preemptive look at Miami's starting lineup, using as our guide Miami's depth chart courtesy of ourlads.com.


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IRVINE CALIF. —After years in Chapter 11 bankruptcy, the once-trend-setting Spectrum Restaurant Group is in the process of selling off the last of its core group of dining concepts, including MacArthur Park, Prego, Guaymas, and Harry's Bar & American Grill. In a return to its roots, MacArthur Park in Palo Alto, Calif., for example, was bought by industry veteran and early Spectrum executive Chuck Frank and the 27-year-old restaurant's ...
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text: Roden Gray | Clothing | T-Shirts | Egyptian Soft Jersey S/S Crew V::Room Egyptian Soft Jersey S/S Crew Details Designer Fit Info Returns Short sleeve crew neck t-shirt in navy Egyptian cotton jersey. 100% Cotton. Regular fit. Front raglan shoulder detail. Rear ribbed hem. Made In Japan. With high quality fabrics and comfort at its core, New York-based label V::ROOM creates simple, casual garments with a great deal of substance and authenticity. Working with a fabric mill in Japan, V::ROOM produces original materials out of the finest fibres from around the globe - Egyptian cotton, Himalayan cashmere, Scottish wool, etc. - to create clothes for people who love and appreciate the quality of basics. The integrity of each piece becomes obvious and integral to the ideology of the brand as a whole, with minimal details allowing for maximum comfort and long-lasting wearability. Model is 6'1"/185cm, with a 36in/91.5cm chest, 29in/74m waist, and weighs 155lbs/70kg. Model is wearing a size Medium. You are welcome to return any items ordered within 14 days of delivery for refund as long as the item is unworn and in new condition. Items discounted 50% or more are final sale and may not be returned. Return shipping is free for customers within Canada. USA and international customers are responsible for shipping charges associated with a return. All returns must be authorized before shipping, please see our full return policy for details. $110.00 CAD $220.00 CAD Final SaleFinal Sale items may not be returned or exchanged.Click for more info. Colour: Navy Select a Size Small | Only One Left Medium | Sold Out Large | Only One Left X-Large | Sold Out TopsBottoms Shoulder Length Chest Sleeve Waist Rise Thigh Inseam Leg Opening


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Staff Picks: Our Favorite Doughnuts
Shipley's always and forever. Their chocolate glazed and bavarian filled were childhood favorites.
Konapun Pizza Video: Watch Adults Make Tiny Inedible Pizzas For No Apparent Reason
It has so much "if only I had this as a child" appeal.
Light and Fluffy Buttermilk Pancakes
Thirty minutes after reading this, my (dieting) roommate and I were feasting on the greatest half-white, half-whole wheat banana pancakes drizzled in agave . Yum! Happy Mardi Gras!
Holiday Giveaway: The Amazing Thermapen Thermometer
A flask of boiling cyclohexanol. Mmmmm.
Ask The Food Lab Anything, Thanksgiving Edition
Here's a question that there is a little more to than meets the eye: What is the difference between a sweet potato and a yam? The internet and my professors disagree, apparently. If given any difference, do they cook the same way and have the same flavor? In which cases (pie, casserole, etc.) would you use one or the other?
Cilantro: Yea or Nay?
Growing up near the Tex-Mex border, I had never even heard of a cilantro aversion until about a year ago. It is an absolute staple around here.
Poll: Which Pizza Substitution Is Least Horrifying?
It's like transplants versus transfusions. Time, patience, and painstaking matchmaking are required for skin and other organ transplants, but given the correct type, blood donations are usually accepted very easily. Likewise, sauce is a less invasive transition than those or dough or cheese.
Candy a Day: Warheads
Black cherry is the best, for sure. I haven't had them since 2003, but the thought of them makes my mouth water not from desire nor nostalgia but from post-traumatic stress. My cousins and I would buy a bag and be done with them in an afternoon, carefully watching each other to ensure that no one succumbed to the pucker face. Those who did were "it" for tag later in the day.
Cookie Monster: Carrot Cake Cookies
These are really great. They also infuse over time, becoming intoxicatingly fragrant, spicy, and scrumptious.


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The irresistible yin and yang of salty and sweet items can be found everywhere now. Chewy caramel morsels sprinkled with imported sea salt are offered in the trendiest candy boutiques, even as good old two-packs of peanut butter cups are waiting for you in the vending machine down the hall. Incorporating sweet and salty flavors into your dessert menu makes an on-trend statement that satisfies customers' cravings — a delicious two-for-one deal. Rise of the 'salt tooth' A 'salty' member of ...
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Alfred Sisley (October 30, 1839 – January 29, 1899) was an English Impressionist landscape painter who lived and worked in France. Sisley was born in Paris to affluent English parents, William Sisley and Felicia Sell. In the early 1860s he studied in the atelier of Marc-Charles-Gabriel Gleyre, where he became acquainted with Frédéric Bazille, Claude Monet and Pierre-Auguste Renoir. Together they would paint landscapes en plein air (in the open air) in order to realistically capture transient effects of sunlight. This approach, innovative at the time, resulted in paintings more colorful and more broadly painted than the public was accustomed to seeing. Consequently, Sisley and his friends initially had few opportunities to exhibit or sell their work, although unlike some of his fellow students who suffered financial hardships, Sisley received an allowance from his father. Sisley's student works are lost. His earliest known work, Lane near a Small Town is believed to have been painted around 1864.
In 1866, he married Eugénie Lesouezec, a Breton, with whom he had two children. His financial security vanished in 1870 when his father's business failed, and Sisley's sole means of support became the sale of his works. For the remainder of his life he would live in poverty; his paintings rose significantly in monetary value only after his death. In 1880 Sisley and his family moved to a small village near Moret-sur-Loing, close to the forest of Fontainebleau where the painters of the Barbizon school had worked earlier in the century. Here, as art historian Anne Poulet has said, the gentle landscapes with their constantly changing atmosphere were perfectly attuned to his talents. Unlike Monet, he never sought the drama of the rampaging ocean or the brilliantly coloured scenery of the Côte d'Azur. Apart from a period spent in London in 1857-61—and brief trips to England in 1874, 1881, and 1897—Sisley lived his entire life in France. Little is known about his relationship with the paintings of J. M. W. Turner and John Constable, which he may well have seen in London, although these artists have been suggested as an influence on his development as an Impressionist painter. Among the Impressionists Sisley has been overshadowed by Monet, whose work his most resembles, although Sisley was less experimental, and tended to work on a smaller scale.
Described by art historian Robert Rosenblum as having almost a generic character, an impersonal textbook idea of a perfect Impressionist painting, his work strongly invokes atmosphere and his skies are always very impressive. His concentration on landscape subjects was the most consistent of any of the Impressionists. Sisley died in Moret-sur-Loing at the age of 59, just a few months after the death of his wife.
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By MICHAEL KELLY
FONDA -- Leading the cheerleading squad at a Fonda-Fultonville football game earlier this season, high school senior Kelsey Gray looks the part.
The head cheerleader's hair is perfect, braided and tied with a maroon-and-gold ribbon. Her white sneakers are spotless. She booms out her cheers through an ever-present smile, the kind that has helped the 17-year-old win beauty pageants in the past.
Cheerleading is one of Gray's two passions. The other? Well, that's wrestling.
"They're both just something I love to do," Gray says.
She's good at both, too. This is her third year being the cheerleading team's captain and she's twice captained the F-F wrestling squad. This past summer, Gray was a key member for the New York entry into the 2013 USA Wrestling Women's Junior Freestyle Duals in Fargo, N.D. Wrestling at 112 pounds, the F-F student helped the New York team to a team championship victory at the event.
"It was so exciting. It was the first time New York had ever placed higher than fourth," says Gray, who went 4-0 in the team competition and 4-2 in the individual tournament.
So excited with its win, the New York team -- which topped California in the finals -- broke the high-rise stage it was standing on when it got announced as the event's winner.
"We were jumping up and down too hard," Gray says.
Usually, Gray is a lot more graceful when it comes to landing her leaps. She does a little bit of everything when it comes to cheerleading, but she is one of the team's flyers. Gray's been cheerleading for F-F since the second grade, when she started tagging along with the older girls at the games.
"I was the little mascot for the longest time," she says.
Now, Gray cheers alongside the team's youngest member, 5-year-old Autumn Purcell, whose big sister, Lexi Baker, is on the squad.
"As soon as Autumn came to practice, though, Kelsey took her under her wing -- and she took a liking to Kelsey," says Kandace Rose, the cheerleading team's head coach.
Gray says the flexibility she possessed from growing up a cheerleader helped her when she decided she wanted to get into wrestling. Her younger brother, Kyle, was into the sport and, at first, she was thrust into the action against her will.
"I'd always be the practice dummy for him; my father would say: 'Come here, Kelsey, I need to show Kyle something,'" Gray says.
Soon, she decided she wanted to go on the offensive. She began wrestling in eighth grade, competing on her own because she was not ready to try to compete for F-F. Competing in tournaments all around the state in her first year, Gray says she wrestled about 60 matches.
"I didn't win a single one," she says.
That changed quick. Joining the F-F team as a freshman and wrestling at 103 pounds, Gray won her first varsity wrestling match.
"It was, like, so exciting," she says, stretching out her "so."
The reaction displays more of Gray's cheerleader side than her wrestling personality type. While she has no problem playing the perky type, a different side of her comes out on the mat. F-F athletic director Eric Wilson says it took him aback the first time he saw Gray wrestle, which was after having her in one of his high school science classes.
"In school, she's a sweetheart. She's just a nice, sweet kid. She's polite and an all-around good kid," says Wilson. "Then, you go to a wrestling match and you see her getting ready ... and that face she puts on, it's like: 'Holy cow.'"
"She's a spitfire," Rose adds.
One of Gray's wrestling coaches had the same experience as Wilson. Mike Paquette helps coach Gray when she trains with the New York Titans, an offseason training group based in Albany. Paquette's first impression of Gray was that the beauty pageants and cheerleading had taken some of the ruggedness from her.
"At first, I thought she was a little soft," says Paquette. "But she's not soft. She's a very determined wrestler. ... I like coaching her. She works hard and wrestlers harder."
Gray says that she's never found the experience of competing in a male-dominated sport too be awkward. With the exception of when she competes with the New York team for national tournaments, she usually is training with and competing against males. Eric Moyer, an F-F wrestling teammate for the past two seasons, says Gray's Braves teammates never really put much thought into the uniqueness of the situation.
"She fits in, it wasn't awkward with her," says Moyer, who graduated in 2013. "It was just an everyday thing going to practice with her."
Gray says she does not get treated differently because of her work on the mat. She says her girl friends think her participation in the sport is cool and that her boy friends are mostly wrestlers, anyway.
"So, we talk about wrestling stuff, like wrestling shoes," she says.
When she says that, she laughs and catches herself, realizing how it sounds.
"Guys talk about their wrestling shoes just as much as girls talk about their shoes," she says, growing animated. "Wrestling shoes are a hot topic."
So is the future. Gray's post-high school goal is to earn a wrestling scholarship to one of the 20-plus college in the country that currently offer women's wrestling. After that, she wants to attempt to compete in the 2020 Olympics in the sport which was recently given second life as one of the games' sports.
"I really hope to get a chance to compete," she says.
Before any of that, though, Gray has a senior season ahead of high school wrestling -- the big goal for her is to make it to state qualifiers -- and a final cheerleading season to finish this fall. Her cheerleading coach says that she just hopes Gray's time flying through the air rooting for the Braves' football team does not harm her upcoming season.
"I just don't want her to get hurt for her wrestling scholarship," says Rose. "She could get dropped or on one of her tumbles she could do something. It's a dangerous sport, believe it or not."
By the looks of it, Gray should be just fine.


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Posts Tagged 'Aimee Nezhukumatathil'
Yi-Fen Chou Is a Real Person, & Everything Else Circling 'The Bees' September 10, 2015: Like a bee to some Venus-flower nectar, the Internet has taken to our poetry scandale du jour. But scandal doesn't cut it. These responses to Michael Derrick Hudson posing as Yi-Fen Chou are [...]
Reading List: November 2013 November 12, 2013: The Reading List is a new feature of the Editors' Blog this year. Each month we ask Poetry's contributors to share a book—or several—that held their interest recently. Here are some [...]
Lucky Fish April 23, 2011: Aimee Nezhukumatathil's third book, recently released by Tupelo Press (which also published Miracle Fruit and At the Drive-In Volcano) affirms her reputation as one of the master miners of the [...]


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Description: This was a school project for an architecture firm called Roof. When it is upside down it creates a house. Name is roof because a roof is the most basic type of shelter.
Status: Student work
Commenting: Actively seeking critiques
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Kazuo Hirai laid down the Sony keynote speech early this morning, cluing the world in to Sony's plan. It goes something like this:
- Further Expansion of the PlayStation 2 business: Sure, us hardcore, up-to-the-minute types might think of the PS2 as yesterday's news, but there are millions of PS2s out there, and even more millions of people who might need a "starter console." So Sony is going to continue to expand on their PS2 success.
- PlayStation Portable: Kaz made it clear that the PSP is a key part of Sony's strategy. He promised the company would "enhance its portability and convenience for users along with continuous cost reduction measures to boost the expansion of the PSP platform," and " Introduce color variations and popular software bundled products." Sony also promised to beef up network content and introduce some unique peripherals such as a camera, GPS and TV Tuner, as well as strengthening the connection between the PSP and the PlayStation 3.
- PlayStation 3: Hirai's plan for the PS3 started with the words any owner wants to hear: Sony plans to strengthening the "Software Development Environment for th PS3," making it easier and more efficient for developers to make games for the system.


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Trailer Talk: This Week In Movies - 'Battle: Los Angeles,' 'Jane Eyre' & More
Last week I hit the movie theaters and I saw the drama "Jane Eyre" and the nerdy comedy "Paul," however due to my agreements with the studios involved I won't be able to discuss them until they are released. Fortunately though "Jane Eyre" comes out this week and "Paul" comes out next week.
In addition to "Jane Eyre," this week you'll have the opportunity to see the alien invasion flick "Battle: Los Angeles," the Catherine Hardwicke thriller "Red Riding Hood," the animated adventure "Mars Needs Moms," the independent drama "Certified Copy," the medieval thriller "Black Death," and the action film "Kill the Irishman."
When a massive alien invasion threatens Earth, a Marine staff sergeant (Aaron Eckhardt) in Los Angeles leads his new platoon into battle against the enemy.
Horror director Jonathan Liebesman, who made films like "The Killing Room" and "Darkness Falls," helms this sci-fi thriller. Christopher Bertolini, the scribe for 1999's "The General's Daughter" pens the screenplay for the movie.
WHO SHOULD SEE IT: Alien invasion junkies should be first in line for this latest attack against the Earth. If you like disaster stories where ordinary people are pushed to the extraordinary to save the human race then you should see "Battle: Los Angeles."
A shy governess (Mia Wasikowska) goes to work at a quiet home in the country, where she develops a close relationship with her employer (Michael Fassbender). What she does not know is that he's hiding a dark secret.
Cary Fukunaga, the man behind the Spanish language film "Sin Nombre," directs this drama based on Charlotte Brontë's frequently adapted novel Jane Eyre. Moira Buffini, the writer of 2010's "Tamara Drewe," adapts Brontë's book for the screen.
WHO SHOULD SEE IT: Anglophiles that appreciate stories set in England during the 1800s should see this drama. If you love period piece stories and you love the novel on which this film is based, then you should see Cary Fukunaga's version of "Jane Eyre."
Valerie (Amanda Seyfried) is a young woman torn between two men; she is in love with a brooding outsider, but her parents want her to marry a wealthy man. Before she can run away though, her sister is murdered by a werewolf. Hungry for revenge, the villagers of her community summon a famous werewolf hunter, Father Solomon (Gary Oldman). Solomon's arrival though only angers the creature, who continues killing people.
Catherine Hardwicke, the woman responsible for the first "Twilight" film, directs this dark thriller. David Johnson, the writer for 2009's "Orphan," tackles the screenplay.
WHO SHOULD SEE IT: Audiences that prefer the dark suspicion and paranoia of murder mysteries during medieval times should see this thriller. If you enjoy Catherine Hardwicke's other films like "Thirteen" and "Lords of Dogtown," then you should watch "Red Riding Hood."
A young boy named Milo (voice of Seth Green) gains a deeper appreciation for his mom (voice of Joan Cusack) after Martians come to Earth and kidnap her. Milo stows away on a Martian spacecraft so that he can mount a rescue mission.
Simon Wells, the great-grandson of famous author H.G. Wells, directs this computer animated adventure. He also collaborates with Wendy Wells on the screenplay, which is adapted from a novel by Berkeley Breathed. Robert Zemeckis produces the film, implementing his famous motion capture style animation.
WHO SHOULD SEE IT: Parents looking for a family friendly story to see with their children should see this movie. If you like similarly animated films like "The Polar Express," then you should check out "Mars Needs Moms."
While in Tuscany to promote his latest book, a British author (William Shimell) meets a gallery owner (Juliette Binoche) who takes him on a countryside tour. Along the way, they engage in a series of intimate conversations on love, life, and art.
Veteran Iranian filmmaker Abbas Kiarostami writes and directs this drama. Interestingly Kiarostami's movie can be seen in three different languages: English, French, and Italian.
WHO SHOULD SEE IT: Independent film fans should definitely see this IFC title. If you appreciate the work of Kiarostami, then you should seek out "Certified Copy."
At the height of the Black Plague, a seasoned knight (Sean Bean) and a young monk (Eddie Redmayne) journey to a village in the country rumored to be untouched by the disease.
Christopher Smith, director of the Irish horror film "Severance," helms this medieval horror movie. Dario Poloni, the scribe for 2006's "Wilderness," pens the screenplay.
WHO SHOULD SEE IT: Viewers with a preference for dangerous quests fought by medieval soldiers should see this. If you like stories that take place during the desperate times when Europeans were terrorized by the Black Plague, then you should see "Black Death."
In 1976, a turf war erupts between Irish mobster Danny Grenne (Ray Stevenson) and the Italian mafia in Cleveland, Ohio as Grenne makes a bid to assert his own dominance.
Action genre screenwriter Jonathan Hensleigh directs this mob movie. Hensleigh also collaborates on the screenplay with first time writer Jeremy Walters. Their screenplay is based on a book by Rick Porrello called To Kill An Irishman. Supporting cast members in the film include Vincent D'Onofrio, Val Kilmer, Christopher Walken, Vinnie Jones, and Paul Sorvino.
WHO SHOULD SEE IT: Action movie lovers should get a kick out of this film about the Irish and Italian mobs duking it out. If you can't get enough of Christopher Walken as a gangster then you should see "Kill the Irishman."


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Sonya Tayeh was born in New York and raised in Detroit, Michigan. She received her BFA at Wayne State university and now resides in LA.
Sonya's career soared commercially when she joined the "SYTYCD" family as a jazz/contemporary choreographer and guest judge. She has directed/ choreographed for many world tours, TV, and awards shows with artists including Florence And The Machine, Kylie Minogue, Miley Cyrus, and many more.
She currently teaches at Edge Performing Arts School, Millennium, and Nuvo Dance Convention and is happy to be teaching contemporary jazz at BDC as well!
Sonya Tayeh Reel


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Director Ben Wheatley Talks 'Sightseers,' Lethal Locations and The Cult Success Of 'Kill List'
With a black sardonic tone and firm grasp on all things unsettling, British Director Ben Wheatley has made quite a name for himself. His turn to the dark side began in 2009 with the cool crime thriller "Down Terrace," which was shot in an amazing eight days. But it was his startling beyond horror outing "Kill List" that announced a cool and original presence in the horror community had arrived. (Not to mention his savory U segment for the recent anthology flick "The ABC's of Death!") His latest effort called "Sightseers" (in select theaters May 10th from IFC Films) continues his tradition of unique and shocking telling the tale of a young couple who go on their first vacation with murderous results. We got a chance to chat one-on-one with filmmaker to watch Wheatley all about making "Sightseers," the lush locations used so memorably in the film and his thoughts looking back on the cult success of "Kill List." Watch out for both the great view and deadly weapon – here's...
There are some interesting stories about how long Alice Lowe and Steve Oram were trying to get the film made and then involvement by Edgar Wright – how did the project come to you and what was your impression upon reading it?
Ben Wheatley: I'd made "Down Terrace," which was my first film, and I got a call from Nira Park and she said to come in for a meeting. She had the script and I knew what it was because I knew Steve and I'd seen their short film version that they did a few years before and that was it really. Basically I said I wanted to do it and I went away to do "Kill List."
There's a much lighter tone to "Sightseers" – was that what made you want to make it after the very dark "Kill List?"
BW: Sure. I didn't want to make the same film again and again and all the films I've made have been very different from each other. Also if you make a really impressive and dark film like "Kill List" and you make another straight afterwards, where do you go, you know? How do you progress from that? So I thought the best way would be to make a comedy after making a horror film – for my own piece of mind.
I read that the locations in the script were places Alice and Steve had previously gone to – were you able to actually go and shoot the film in the same spots?
BW: Yeah. There have been questions about this – whether or not the locations knew what they were getting into. But they did. You can't make films and not tell people what you're going to shoot there – it doesn't work like that. So we showed them bits of the script and explained what was doing and everyone was cool with it.
It's funny how Steve's character seems more the aggressor but the tables begin to turn towards Alice – whom do you think is more dangerous overall?
BW: I think they've got their moments – both of them. What interested me about the story is that it was a guy who got ahold of an impressionable woman and thought that he could control her only to find out that she's not to be controlled. And that the thing that he thinks he's good at she's actually much better at – it's just a shame that the hobby he has isn't hand gliding or something less murderous.
We're you at all surprised by the cult success of "Kill List?"
BW: Yeah. I don't think you can ever take for granted what the reaction of the audience is going to be. You'd be a terrible egotist if you went into it with 'this is going to rock everyone's work' and 'this is going to be fantastic' – you go in cautiously and hope for the best. But I was really happy with what happened. We made the film completely from our perspective in terms of we made the film we wanted to make and we liked it. We hoped other people would like it, but we didn't presume that that would.
You also made the U segment for "The ABC's of Death" – were you happy with it and the overall film as a whole?
BW: Yeah, it was a chance to work with a lot of people I'd worked with before and get to play. It was good to get out and try some stuff. And the other thing was I got to do a little vampire film and in my career I might not get to do that, so that was a real treat. In terms of the whole film (laughs) I've never seen it. But it' a great idea – twenty-six little feature films from around the world.
Can you talk a bot about your next projects "A Field in England" and "Freakshift?"
BW: "A Field in England" we just completed and Amy Jump wrote that. And we've had one screening so far for friends and family and it's gone really well, so we're excited about that. And hopefully "Freakshift" is gonna go at the end of the year.


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The Eloora Shorthorn herd is run
The stud founded in 1959 is a family concern, run by
Ray & Jill Brook and Dion & Hannah Brook. The Eloora herd
was originally founded on Kelso bloodlines, however in more recent years
genetic acquisitions have come from the Spry's and Marellan herds. In
addition to the registered cattle, an intensive prime lamb operation is
run, targeting the heavy export market. As with the cattle, the
The principals of the breeding operation at Eloora have for many years been targeting towards producing genetics for clients that will return them maximum profitability. Ray believes today's Shorthorns "should have the capability of being sold to a wide range of markets at any age of their lives, with seasonal problems and market fluctuations becoming an ever increasing and regular concern, it is paramount to produce cattle with market flexibility".
Running approximately 100 females with an Autumn calving Eloora has bulls, females
Eloora Baker G31, semen for sale
> Beef Week - Day 8, Wednesday 3rd February
|About Us | Sires | Sale Bulls | Contact Us | News | Home Site designed by Squish Creative


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I get oxygen treatment facials from Intraceuticals whenever I can– at least once a month and weekly if I have time (hey, don't judge– Madonna gets them DAILY!). The oxygen infused Serum contains lightweight Hyaluronic Acid and a powerful combination of vitamins and antioxidants that penetrates the deepest layers of the skin, making my skin tone bright and texture refined. They recommend a series of 6 weekly facials and then monthly maintenance but since I am so flaky, I've never done the entire series so I'm on the perpetual program which is fine by me cuz I love the way my skin looks afterwards!
Last week, I tried the newest treatment from Intraceuticals– CLARITY, which addresses adult acne. It's not a huge problem for me but every so often I do get a blemish that drives me nuts! I mean how annoying to go through puberty without the body of a 16 year old! Seriously though, I end up picking at the blemish or squeezing it too early which leads to– you guessed it, scarring! For those with a severe acne problem, there is a Clarity infused oxygen treatment but I only need the Clarity pen for my occasional break outs. It's a genius product– a disposable unique applicator pen containing a concentrated formula to target acne blemishes and speed up the healing process while reducing redness. The applicator tip is designed to be replaced daily to avoid contamination and bacteria. It comes with 15 tips and you can purchase replacements as necessary.
Use as required or twice daily onto blemishes and watch them disappear without a trace! I know this sounds crazy but I can't wait for my next adult pimple so I can zap it with this pen!
I get my Intraceuticals treatments and products at Ritz Carlton Spa in Dallas (214) 922-4820
If you don't live in Dallas– go on their website to find a location near you. Intraceutical oxygen infusion treatment is a MUST in your beauty regimen!


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LAKE FOREST, Fla. - Dr. Robert H. Messer, 71, died Wednesday, Aug. 13, 2003.
Memorial services will be at 11 a.m. Saturday in St. Andrews Chapel in Sanford with Dr. R.C. Sproul and the Rev. Tim Bonarrigo officiating. Arrangements are by A Sunset Cremation Service.
After a courageous battle with a malignant brain tumor, Robert H. Messer went home to be with his Lord on Wednesday at the age of 71.
He was born in Ludington, Mich., on Oct. 21, 1931. He went to medical school and residency at the University of Michigan. He was a Fellow in the American College of Obstetrics and Gynecology. He served in the Air Force, and was honorably discharged in1972 having achieved the rank of captain.
Bob should be remembered for his dedication to the medical education of hundreds of medical students and Ob/Gyn residents, his impact on the lives of his professional colleagues and his kind and compassionate care of his many patients.
Upon his retirement in 1997, he was awarded the Texas Tech University Health Sciences Center Distinguished Faculty Service Award and was appointed professor emeritus in the Department of Obstetrics and Gynecology.
During his 35-year career he served as chairman of the Ob/Gyn departments in the schools of medicine at the University of Nebraska, the University of New Mexico and Texas Tech University.
He marrid his wife, Dianne, in 1990. They have been Florida residents since 1997.
He was preceded in death by his parents, Harry and Mary Messer and his first wife, Emily Baker Messer.
Survivors include his wife; a brother, John and wife, Carol; two sons, Robert H. and wife,Lisa, and Michael Kimmons; three daughters, Karen Christensen and husband, Kirk, Laura Anderson and husband, Jon, Ellen Wright and husband, Glenn; nine grandchildren, Emily and Lindsay Messer, Tyler, Kyle and Hannah Christensen, Ana, Caroline and Brian Neff and Alex Anderson; his mother-in-law and father-in-law, Muriel and Hobert Miracle; two brothers-in-law, David Folden and Barry Bickle and their families; a sister-in-law, Kathee Bickle; and many nieces and nephews and numerous dear friends.
The family suggests memorials be to the building fund of St. Andrews Chapel, 514 Walden View Dr., Sanford, FL 32771, or Hospice of the Comforter on Montgomery Rd., Altamonte Springs, FL 32714.
Amarillo Globe-News, Aug. 22, 2003
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