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Thai custard
Recipes / Thai custard (1000+)
Thai Coconut Custard
480 views
Thai Coconut Custard, ingredients: 8 lrg Large eggs, 1/2 c. brown sugar
Apple Custard Meringue
593 views
tsp cinnamon, 2 c. stewed apple, 1/2 pt egg custard, 2 x egg whites, 2 Tbsp. sugar
Coconut Custard
556 views
Coconut Custard, ingredients: thai, 400 ml coconut cream, 120 gm white sugar, 6 x
Deep Fried Buns With Custard Paste
791 views
butter, 240 gm sugar, 25 gm flour, 25 gm custard pwdr, 25 gm corn flour, 2 x Large eggs
Egg Custard Cake
1034 views
Egg Custard Cake, ingredients: 1 pkt Egg custard mix, (3 ounce. box), 2 x Large eggs, 1 1
Lumpless Custard
553 views
Lumpless Custard, ingredients: 3 x rounded Tbsp. Custard Pwdr, 1/2 c. small Sugar, 1 c
Thai Ho Mok Pla aka Steamed Curry Fish Custard
109 views
we love about Thai food. Once spoonful of this fragrant curry fish custard and you might
Egg Custard & Chocolate Croissant Pudding
668 views
on the base and three on top MAKE CUSTARD -Get a stainless steel saucepan and a large
Fresh Fruit Salad with Custard Apple Fruit
180 views
I purchase my young Thai coconuts from all theand custard (hence the name Custard Apple
Sticky Rice with Mango
58 views
these days I'm going to try to make Thai custard to go with it when good mangoes are not
Groups / Thai custard (0)
S.E. Asian, including Thai
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S.E. Asian, including Thai, Thai, Vietnamese, Cambodian, Laotian, Indonesian, Malaysian
Spicy food club
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If you like spicy food, join us. Peruvian, Thai, Vietnamese, Indian, Sri Lankan, Mexican
Thai food
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Asian Cuisine
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Personally, I can never get enough Thai, Chinese, Japanese, Vietnamese, Indian, and of
Themes / Thai custard (0)
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and Thailand? Then this is the group for you!, cuisine: Thai
Trusted Results with Thai custard
thai custard Articles at Epicurious.com
We are unable to find an exact match for: thai custard. ... Learn to make this classic British sponge cake with custard sauce in this episode of Around the World in 80 ...
coconut thai custard Recipes at Epicurious.com
show results for food recipes drink recipes member recipes all recipes ... Coconut Custard Pie Gourmet, February 2003. Coconut Cream Tart Gourmet, ...
thai coconut custard Recipes at Epicurious.com
Thai (3) dietary consideration " select from the following: Low Fat ... Thai Coconut Tapioca Pudding with Cayenne-Spiced Mango Bon Appétit, January 2006 ...
Caramelized Peach and Custard Tart. Clip this. Rated -9223372036854775808 stars out of 5 ... Virgin's Breasts (Candied Fruit Custard Tarts): Seni di Vergine. Clip ...
Fried Mascarpone Custard with Strawberry Tawny Port Sorbet and Valrhona Ganache ... Baked Caramel Custard. Clip this. Rated 4 stars out of 5. 3 ratings ...
Steamed Egg Custard Recipe : : Food Network
Food Network invites you to try this Steamed Egg Custard recipe.
custard filling : Food Network
We found 111 results for "custard filling" Showing 1-12 of 111. Sort by: 1. Blueberry ... Thai (1) South American (1) Cuban (1) Jewish (1) Swiss (1) Central ...


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Fri, 2011-11-04 06:41
Hey yall,I am sponsored and sit for my series 7 in a month. I keep worrying about the fact that I have a little portfolio (under 10k) that I place a trade about 10 times a year in. Will this cause any troubles now or after I am a rep? Thanks and so glad I found this forum!

subset: commoncrawl


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Serious Sam Enemies
A selection of articles related to serious sam enemies.
Original articles from our library related to the Serious Sam Enemies. See Table of Contents for further available material (downloadable resources) on Serious Sam Enemies.
- Serious Cleansing Ritual
- This cleansing ritual has been modified from one that I performed for a dear friend who was having trouble dispelling negativity possibly sent from a former romantic acquaintance. This is not a ritual to perform for just anybody - the performer actually...
Paganism & Wicca >> Spells
- King James Bible: Leviticus, Chapter 26
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Old Testament >> Leviticus
- King James Bible: Deuteronomy, Chapter 28
- Chapter 28 28:1 And it shall come to pass, if thou shalt hearken diligently unto the voice of the LORD thy God, to observe and to do all his commandments which I command thee this day, that the LORD thy God will set thee on high above all nations of the earth:...
Old Testament >> Deuteronomy
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>> Psychic Abilities
- Dynamics & Bioenergetics Of OBE Sex
- Once the exit out-of-body has been mastered and projectors begin tentatively exploring their new environment, another natural barrier will soon raise its ugly head. As projectors begin expanding their horizons they will soon begin encountering other...
Astral Projection >> Out of Body Experiences
- King James Bible: Deuteronomy, Chapter 20
- Chapter 20 20:1 When thou goest out to battle against thine enemies, and seest horses, and chariots, and a people more than thou, be not afraid of them: for the LORD thy God is with thee, which brought thee up out of the land of Egypt. 20:2 And it shall be,...
Old Testament >> Deuteronomy
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Great care has been taken to prepare the information on this page. Elements of the content come from factual and lexical knowledge databases, realmagick.com library and third-party sources. We appreciate your suggestions and comments on further improvements of the site.
Serious Sam Enemies Topics
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A public shooting range planned for an area near the Denver International Airport could endanger planes approaching or departing from the airport, according to airport and Federal Aviation Administration officials. Meanwhile, county officials say they have addressed safety concerns. Denver International Airport planning manager Jeannette Stoufer said that aircraft approaching the airport would typically be at an altitude of about 500 feet above ground level over the proposed shooting range. She also said the shooting range would require frequent monitoring by the Department of Homeland Security.
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There's still at least one visit to go, but Patrick Beverley is closing in on a decision. Michigan's chances seem to have been enhanced after a visit last week that, to the public eye, seemed to go...
Beverley visit should help U-Ms chances
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SUBSCRIPTIONS for our 38th Season 2016-2017 are now available online!
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The Beat Goes On's Top 10 albums of 2011
Making a Top 10 list is hard. Not busting-up-concrete kind of hard, but in an instant-remorse kind of hard. It never fails —the minute it's unchangeable and I'm locked in, I inevitably remember something that I wish I'd put on the list or a great new album comes out after my deadline (for me, that was Anthony Hamilton's "Back To Love"). Then other top 10 lists make me realize how many albums I was unable to give a full and fair listen to in their totality, even when I liked the individual tracks I heard. Among the albums in that category this year are Wild Flag's self-titled set, Cut Copy's "Zonoscope," The Weeknd's "House of Balloons" and tUnE-yArDs' "whokill."
In addition to serving as reminders, best-of lists also provide the opportunity for music discovery. I get great pleasure out of looking at other people's lists and learning about albums that I didn't have time to get to at all. Among the albums that I spent no time with this year that are showing up with some regularity on other lists and I have now earmarked for future listening include Fucked Up's "David Comes To Life," Shabazz Palace's "Black Up," and Toro y Moi's "Underneath The Pine" (despite the album cover that creeps me out).
So with all that said, bear in mind that these are my favorite albums that I listened to in 2011. I make absolutely no claim that they are the best albums of 2011, and I know for sure that my selection at No. 10 made no other lists. Plus it sounds totally different from everything else that comes after it in my Top 10. These were the albums that I found myself returning to over and over again whether I needed a pick-me-up, a good workout on the treadmill or just a musical companion during my journey.
My colleague Katie Hasty has already posted her video of her top 10 singles (and 40 more that she liked) and it's chock-full of great stuff. I'll post a text list of my top 10 singles over the next few days.
So watch the video, tell me what I got wrong while admiring HitFix's own Alex Dorn's great editing work. Then tell me what you liked this year that I missed.
If you don't want to watch the video, here's the list.
10) Pitbull, Planet Pit
9) Little Dragon, Ritual Union
8) Raphael Saadiq, Stone Rollin'
7) The Low Anthem, Smart Flesh
6)The Civil Wars, Barton Hollow
5) Dawes, Nothing Is Wrong
4) Paul Simon, So Beautiful or So What
3) Foo Fighters, Wasting Light
2) Adele, 21
1) Glen Campbell, Ghost on the Canvas


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Labor Day Holiday
Monday, September 03, 2012
Where and When
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Number Line 1
These number lines can be used by children throughout the other decimal activities in this section. They will be particularly useful when adding and subtracting decimals and when rounding them to the nearest unit / tenth.
There are three sets of number lines, which are all available in PDF format. Each set contains more than one number line (so that you can cut them up so that each child can have one line - this will hopefully reduce photocopying costs).


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Here's your dumb media theory of the day. Fark is the center of the "weird news" universe. The editors churn through over 2,500 submissions a day to find the strangest news stories, and then label them with tags like "unlikely," "fail," "amusing," and "Florida." Yes, Florida is the only state with its own dedicated Fark tag due to the unusually high number of stupid people doing stupid things. Or at least the unusually high number of stupid journalists covering stupid people doing stupid things.
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Upcoming Events
Miami Herald: Beware of 'phantom loads'
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Sun-Sentinel: You think palmetto bugs are big? New 'hissing' cockroach may head to South Florida
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Kind of just makes you wonder if the local media is a little to aware of the Fark-bomb incentive when covering the "news."
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Sofitel Brussels Le Louise hotel
40 Avenue de la Toison d Or
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The Sofitel Brussels Le Louise is a 5-star hotel designed by famous architect Antoine Pinto to reflect light and create a theatrical and stylish atmosphere. Located in the exclusive Avenue Louise area, with the best luxury boutiques, the Grand Sablon andthe Grand Place just moments away. Our rooms and suites with My Bed and our Crystal Lounge with its beautiful Terrace offer an unforgettable traveling experience. Surrealist Meeting rooms available. Eurostar station 5 minutes away. Parking
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28 m² (301 sq. ft.), Stylish and sophisticated, feather MyBed, high floor, complimentary WiFi, rain shower (bathtub upon request), Nespresso coffee machine, laptop safe, Bose® wave radio, flat screen TV and complimentary minibar.
24 m² (253 sq. ft.), Stylish and sophisticated, feather MyBed, complimentary WiFi, rainshower (bathtub upon request), Hermès toiletries, tea and coffee making facilities, laptop safe, Bose® wave radio, flat screen TV and minibar.
37 m² (398 sq. ft.), Stylish room, garden view, feather MyBed, lounge area, Nespresso machine, Bose® radio with iPod dock, complimentary high-speed Internet, bathtub and rain shower, 2 flat screen TV's, laptop safe and complimentary minibar.
92 m² (990 sq. ft.), Opera Suite, courtyard views and city views, spacious and cosy lounge, rainshower and bathtub, hammam, Jacuzzi, fitness equipment, complimentary high-speed Internet, relaxation area, Wii entertainment console.
50 m² (538 sq. ft.), Stylish room, garden view, lounge area, dressing room, Nespresso machine, Bose® wave radio with iPod dock, complimentary high-speed Internet, bathtub and rain shower, 2 flat screen TV's, laptop safe and complimentary minibar.
Award-winning chef Adwin Fontein inspires gourmet creations - from foie gras to monkfish - in this sleek restaurant in Brussels. Soak up the Zen atmosphere of a leafy terrace with a relaxed Sunday brunch or cheeseburger paired with a Belgian beer.
Antoine Pinto's theatrical style adds spice to this chic Brussels bar: amethyst crystal with chrome details, chocolate leather banquettes on hardwood floors. Transform a pre-dinner drink or power lunch into a moment of refined refreshment.
Reviews collected from customers who booked on AccorHotels.com
Staying at the Sofitel Le Louise certainly enhanced our stay in Brussels. Well situated as an excellent base for exploring the city not only on foot, but with good access to underground and rail links. A day trip to Waterloo was easy to organise. The quality of service in the hotel was consistently outstanding. During our four night stay, all staff we encountered were friendly, courteous and very professional, nothing was too much trouble. The well appointed room on the upper floor was excellent, light and airy, and we experienced very little noise. The comfort of the bed (the mybed system is excellent) guaranteed deep and peaceful sleep. We look forward to our next Sofitel stay in Brussels.
Stayed there one night for a business trip. Everything was in perfect order: staff very friendly and helpful, room in excellent shape and clean and the breakfast was really good. The location was also extremely convenient and central.
Hotel facilities & amenities: Nothing to brag or rant about. A very standard 4 start hotel. No bathtub even in the highest grade premium room. The walk-in rain shower is a pleasant substitute though. Location: Great location if you're in Brussels mainly for shopping. All well-known designer brands are within walk distance - LV, Prada, etc. There's even an Apple Store. However, if you're in Brussels for sightseeing it's not great. A good 25 walk to Grand Place where the major sights of interest are located. The really bad thing While I was sound asleep on the first night, one of their staff decided to make another 100Euro charge to my credit card. I got woken up by an alert sent by American Express. This was in addition to the 100Euro they had already charged me for guarantee at check-in. My room was prepaid upon reservation. The next morning I asked the reception about this. First they denied any knowledge. After I showed them the transaction record on my phone, they said it was a mistake and they would refund me. "It would be OK" they said. When I was checking out however, no refund had been issue. Even worse, they reception denied any knowledge of any incident. I spent about 15 minutes explaining to them and show them the transaction alert on my phone as proof. Only after a lot of pushing did they admit their mistake and process the refund. I asked a manager to contact me and give an explanation. All I received was a very cold an unhelpful email from Charles the Chief Reception to say he was "quite sorry" that hey had charge my credit card without authorisation. I subsequently wrote a separate email to Sofitel Customer Service. After one week still no reply. Advice for staying at Sofitel Brussels Le Louse Check your credit card transaction carefully during and after your stay. They will charge you behind your back without authorisation and it will be a pain for you to prove and get your money back!
It was my first time at this hotel and I quite liked the overall experience. The staff (except for one lady at the reception) was kind and useful. The hotel is nicely designed (although not very recent). The room I was upgraded to was relatively spacious and very comfortable (nice wardrobe, great rain shower). However, I did not like a number of things: I was not explained the facilities/services of the hotel upon check-in; the phone in my room was broken (you could only use the loudspeaker) and although I reported it, nothing was done to fix the problem (e.g. repair it or replace it); my room was facing the main road in front of the hotel (Avenue Toison d'Or) and you could hear the traffic through the large windows; the bar is open to outside guests and was very busy leading to long waiting time and errors by the barman even for a simple beer; last, but actually most important, I really did not find the bed very comfortable. They have introduced a new bed concept they seem to be proud off, but they actually topped the mattress with an additional layer which makes the bed surface everything but not flat... If I was staying longer, I would have asked to remove it.
What I liked most about my first stay here was the friendly receptionist who was happy to extend my checkout when, at around 5am, I asked him if this were possible. My room was ok but a little less than what I expected from the Sofitel brand. My checkout was disappointing. The receptionist made a number of mistakes in billing me and at no point did she apologise. As I already told the hotel, mistakes are ok but should be accompanied by an appropriate apology.
We Absolutely enjoyed our stay at Sofitel Brussels, we came from Paris to Brussels, and this hotel is very easy to find ! We checked in in less than 5 min, staff were attentive and wonderful! Room was clean, modern and stylish ! I love the decor of the hotel , welcome drinks in the reception area were wonderful (the lemon orange cinnamon water ) was delicious ! I jusr couls t stop drinking when everyone I walked by reception area! Location was excellent, since we are family of 3, and travelled with a 13 mths baby, this location was excellent for us and baby, coz we can always walk back to hotel to take a break, if u enjoy shopping like I did , then this is the hotel u should stay in!
Dear Guest, We would like to thank you for your outstanding review on TripAdvisor. We are thrilled that you "absolutely enjoyed" your family stay with us, and that you appreciated the location as well as the decor of the hotel. Travelling with children is not always an easy task. Therefore, we are really pleased if we made it easier for you. We look forward to welcoming you back soon. Best Regards, Sophie Blondel, General Manager, Sofitel Brussels Le Louise
The best hotel I've stayed at in Brussels. The service was excellent. Food very good and tasteful. Rooms discretely decorated and well equipped. I'll definitely return as long as the budget allows it. The surroundings full with nice places to eat and shop if needed.
Dear Guest, Thank you very much for your lovely review on TripAdvisor. We are delighted that you had a "superb stay" and that you found our service "excellent". It will be a pleasure to welcoming you back again, and we will do our best to remain your favorite hotel in Brussels. Best Regards, Quentin Guiraud, Marketing Coordinator, Sofitel Brussels Le Louise
it is not my first stay at this hotel which i like it, i am using this hotel for the last 6 years but honestly they didn't upgrade my room during all this period even i am a platinum member and honestly i cannot find their excuses logic(same reason always that the hotel if fully booked). special thanks for the concierge department especially Mariano and Jefferson which are excellent. Really i don't know if i will use the same hotel(ranking 10 as TripAdvisor) but for sure i will use Sofitel brand which will be sofitel Europe in Brussels(ranking 5 TripAdvisor).regards
Dear Guest, I thank you for taking the time to tell us about your experience at the Sofitel Brussels Le Louise. At Sofitel, we strive to provide a superior service experience for every customer; therefore your feedback is very important to us. It draws our attention on the possible dysfunctionings within our organisation and help us to take the right measures to always provide the best service and quality to our guests. I am very sorry that your experience with us didn't reach your expectations and I express my sincerest apologies for this. I appreciate your sharing your concerns, and it is my hope that you will give us the opportunity to better serve you in the near future. Sincerely Yours, Anita Piercot Assistant Manager
This hotel is 10 min away from thalys mid station and 20 min away from the airport. What more than this ? Well this hotel is located right bang in the main shopping street of Brussels. Walk beside you get a mall and all kind of fashion shops located right opposite of this hotel. For food we just need to walk 5 min and you have all sort of foody world including EKKI which is one of my fav as it has amazing vegy snacks. All the major historic places are just 15 to 20 min walking distance and you regret the walk as you get to explore those architectural houses and buildings otw. Dont forget to visit TINTIN museum again all near by this hotel. Coming to the hotel we stayed here for 4 nights before we left to greece and again 4 nights after done with greece. We made this hotel as our base to visit other places like Antwerp and even Rotterdam. The hotel staff are really helpfull especially the concierge ( forgot his name ) But an amazing service which pleased me to the best. The only regretful thing was we had to request for mini bar to be filled every single day :) which was quiet annoying and the worst was when we arrived from greece late in night we were given a room in 1st floor which had the dirtiest bath tub and they changed the room to the beside well it was no different but the reception made some quick moves and gave us the same room which we had stayed before which was facing the main street which was amazing. But over all this hotel is worth staying.
Dear Guest We very much appreciate your comments regarding your stay at the Sofitel Brussels Le Louise. It is always a pleasure to receive positive feedback. Thank you for sharing your experience, and we hope your next visit will be as pleasant as the previous one. Sincerely. Sophie Blondel General Manager
BEDS are heavenly and cloud like ! Lovely room for a first time visit to Belgium. Restaurants suggestions from the concierge team were amazing , staff were helpful and acknowledge a birthday of my boyfriend. Breakfast selection was lovely and refreshed frequently and food was fresh and well presented.
Dear Guest We very much appreciate your comments regarding your stay at the Sofitel Brussels Le Louise. It is always a pleasure to receive positive feedback. Thank you for sharing your experience, and we hope your next visit will be as pleasant as the previous one. Sincerely. Sophie Blondel General Manager
We were very impressed by the highly motivated and friendly staff whatever their role.Although this is a very impressive and sophisticated hotel, it was a lovely place to rest after a busy day of helping family with a move to Brussels! At one point during our 4 day stay, we had our grand daughter staying at the hotel and she was treated so well, and she loved the gold balloon which she was given by one of the breakfast staff. Once again, thank you to the excellent team at Sofitel Louise. Thank you especially for the advice given to us by the concierge, which helped my daughter with her registration at the commune! We also appreciated the loan of an excellent umbrella! We hope to return soon!
Dear Customer, We are delighted to read your lovely comment, and would like to thank you for having taken the time to share your story on TripAdvisor. It was a real pleasure welcoming you at Sofitel Brussels Le Louise, and we are pleased to read that you were fully satisfied with the service you received from all our staff. Making sure you feel at ease is our priority and we are glad to have correctly responded to your requests. We will be glad to welcome you again soon in our property. Warm Regards, Quentin Guiraud Marketing Coordinator - Sofitel Brussels Le Louise
The hotel is very comfortable and meets all the usual Sofitel expectations including a wonderfully comfortable bed. The location next to the Louise metro makes the hotel easily accessible to other places in Brussels. The concierge was particularly helpful and the staff all friendly and efficient.
Dear Guest, Thank you for sharing your Magnifique comments and feedbacks regarding your recent stay at the Sofitel Brussels Le Louise. We are delighted to read that you were satisfied with your stay, especially with the conciergerie services. Our staff strives to provide the best service and we promise to maintain and continuously improve the standards of the hotel. Looking forward to welcome you back soon for another Sofitel experience ! Kind regards, Anita Piercot Assistant Manager
When making a booking at this hotel:


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Oxytocin is synthesized and released in the heart and vasculature, tissues that also express oxytocin receptors. Although it has been established this intrinsic cardiovascular oxytocin system is important in normal homeostatic cardiac and vascular regulation, a role for this system in cardiovascular pathophysiology has not been investigated. The current study examined the influence of oxytocin on mechanisms in atherogenesis, oxidative stress, and inflammation in cultured human vascular cells, THP-1 monocytes, and macrophages. Oxytocin receptor protein and mRNA expression, NADPH-dependent superoxide activity, and interleukin-6 secretion were measured. Results demonstrated oxytocin receptor protein and mRNA in THP-1 monocytes and macrophages. Incubation of cells at physiological levels of oxytocin significantly decreased basal and stimulated NADPH-dependent superoxide activity in vascular cells, monocytes, and macrophages by 24–48%. Oxytocin also attenuated interleukin-6 secretion from stimulated THP-1 macrophages and endothelial cells by 56 and 26%, respectively. These findings suggest that oxytocin attenuates vascular oxidative stress and inflammation, two important pathophysiological processes in atherosclerosis. The fact that oxytocin receptors are found in monocytes and macrophages, and oxytocin decreases both superoxide production and release of a proinflammatory cytokine from these cells, suggests a potentially larger role for oxytocin in the attenuation of disease.
- reduced nicotinamide adenine dinucleotide phosphatase oxidase
- interleukin-6
- atherosclerosis
the neurohypophysial peptide oxytocin is involved in peripheral female reproductive functions, including uterine contraction and lactation. Oxytocin, acting in the central nervous system of both males and females, additionally plays an important role in a variety of complex social behaviors in mammals such as affiliation, sexual behavior, social recognition, socially stressful behavior, and aggression (4, 8, 36). It has also been proposed that oxytocin is a cardiovascular hormone that plays an important role in normal homeostatic mechanisms (11). Oxytocin is synthesized and released in the heart and vasculature of rats (14, 15), and these tissues also express oxytocin receptors (11, 15). This intrinsic oxytocin system in the heart stimulates the local release of atrial natriuretic peptide that slows the heart and decreases cardiac contractility, producing a reduction in circulating blood volume (11, 14, 15). It has been proposed that vascular oxytocin regulates vascular tone as well as vascular regrowth and remodeling (15). Cultured human vascular endothelial cells and aortic smooth muscle cells express oxytocin receptors (33, 37). Stimulation of these cells by oxytocin produced an increase in intracellular calcium, release of nitric oxide, and a protein kinase C-dependent cellular proliferative response (33).
The homeostatic functions of the intrinsic cardiovascular oxytocin system are beginning to be understood. However, the potential role of oxytocin in cardiovascular pathology has not been investigated. Increased vascular oxidative stress and inflammation are known to be critical early events in the progression of atherosclerosis (10, 29, 35). The primary vascular oxidative stress pathway is through NADPH oxidase, found in endothelial cells, smooth muscle cells, and macrophages within the vessel (35). Early events in atherogenesis involve monocyte interaction with the endothelial cell layer, migration into the subendothelial space, and differentiation into macrophages (29). Once present in the vessel wall, macrophages release a variety of chemokines and cytokines that propagate the atherogenic process. In wound healing and sepsis-induced animal models, subcutaneous injections of oxytocin were shown to have antioxidant properties and modulated the immune and anti-inflammatory response (6, 12, 13, 28).
The current study was designed to test whether oxytocin modulates oxidative stress and inflammatory response by vascular cells and macrophages, thereby providing a mechanism that may slow the progression of atherosclerosis. The goals of this study were to identify the presence of oxytocin receptors in cultured human vascular cells, including monocytes and macrophages, and examine the influence of oxytocin on vascular oxidative stress and inflammation in these cultured cells as measured by NADPH oxidase activity and IL-6 secretion, respectively.
Oxytocin was obtained from Phoenix Peptides (Belmont, CA). Lucigenin, NADPH, tumor necrosis factor-α (TNFα), phorbol myristate acetate (PMA), tiron, diphenyleneiodonium, and rotenone were from Sigma (St. Louis, MO). Reverse transcription reagents were from Roche Applied Science (Indianapolis, IN). TaqMan gene expression assays were from Applied Biosystems (Foster City, CA). Primary human aortic endothelial cells were from Invitrogen (Carlsbad, CA), and aortic smooth muscle cells were from Lonza (Walkersville, MD). The human THP-1 cells are a cell line derived from a patient with acute monocytic leukemia (34) and were from American Type Culture Collection (Manassas, VA).
Cell cultures.
Endothelial cells were cultured in Medium 200 supplemented with low-serum growth supplement (Invitrogen). Smooth muscle cells were cultured in basal medium (Lonza) containing supplements and growth factors at 37°C in a humidified 95% air-5% CO2 incubator. Cells were used between the third and eighth passage. For experiments, cells were plated into 35- or 60-mm dishes at 25,000 or 50,000 cells/dish and grown until ∼80% confluent.
THP-1 cells were maintained in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum, 2 mM glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin, and 0.05 μM mercaptoethanol. Cells were seeded at 100,000 cells/well in 24-well tissue culture plates or into 60- or 100-mm dishes at 1 × 106 or 5 × 106 cells/dish, respectively, and differentiated by adding 100 ng/ml PMA for 24–48 h and maintained for 7–8 days before experimental manipulation.
Cells were incubated with oxytocin at physiologically relevant levels (10–100 pM) on the basis of studies reporting plasma oxytocin concentrations of 5–250 pM (1, 20, 27).
Oxytocin receptor protein expression.
Oxytocin receptor expression was examined in cell membrane fractions. Phosphate-buffered saline (PBS)-washed cells were collected by scraping and suspended in lysis buffer (15 mM KCl, 1.5 mM MgCl2, 10 mM HEPES, 1 mM dithiothreitol, 250 mM sucrose, pH 7.4). Cells were homogenized using a dounce homogenizer then centrifuged for 5 min at 800 g. The resulting supernatant was centrifuged for 10 min at 10,000 g at 4°C to remove subcellular fractions. The supernatant was then centrifuged for 60 min at 100,000 g at 4°C to obtain membrane-enriched pellets. The pellet was solubilized in SDS buffer (50 mM Tris, pH 8.6, 1% SDS), aided by brief sonication. Protein was measured with BCA Protein Assay (Pierce, Rockford, IL).
For immunoblotting, 10–20 μg of protein was separated by denaturing and reducing electrophoresis on 10–20% Tris-glycine polyacrylamide gradient gels (Lonza). After separation, proteins were transferred to nitrocellulose. Membranes were blocked in Tris-buffered saline containing 5% nonfat powdered milk and 0.1% Tween-20 for 1 h and then probed with polyclonal anti-goat oxytocin receptor (ADI, San Antonio, TX) diluted in blocking buffer (1:200) at 4°C overnight. Immunoreactive bands were detected with appropriate peroxidase-conjugated secondary antibody for 1 h and then visualized with chemiluminescence. To normalize for protein loading, the membranes were stripped and reprobed with anti-actin antibody.
Deglycosylation of oxytocin receptor.
THP-1 macrophages were collected, and membrane pellets were prepared as described above. Peptide N-glycosidase (PNGase-F; Sigma) was used for the deglycosylation of the mature oxytocin receptor overnight at 37°C. The enzyme reaction was terminated by addition of SDS buffer. The samples were subjected to electrophoresis and immunoblotted with oxytocin receptor antibody.
PCR experiments for oxytocin receptor.
Oxytocin receptor mRNA expression was evaluated by polymerase chain reaction (PCR). Total RNA (optical density ratio of 260/280 nm, >1.8) was isolated using the RNeasy kit (Qiagen, Valencia, CA) and stored at −80°C. After treatment with DNAse I (Applied Biosystems), the reverse transcriptase reaction was carried out by mixing 5–10 μg of total RNA in the presence of random oligomers (3.2 μg), MgCl2 (5 mM), deoxynucleotide mix (1 mM), AMV reverse transcriptase (≥20 U), and RNase inhibitor (50 U) and incubated at 25°C for 20 min, then at 42°C for 60 min, and then denatured at 99°C for 5 min and then cooled to 4°C for 5 min, resulting in cDNA synthesis.
Quantitative gene expression of human oxytocin receptor with the use of real-time PCR was performed with the TaqMan gene expression assay. Fifty nanograms of cDNA was amplified with TaqMan Universal PCR Master Mix and reactions run using universal cycling conditions on an Applied Biosystems 7500 Real-Time PCR system. Samples were analyzed in triplicate. The ΔΔCT (threshold cycle) method was used to analyze changes in gene expression in a given sample relative to another reference sample and expressed as the fold change in gene expression. 18S RNA was used as endogenous control. A no-template control was performed to ensure that there was no amplification of genomic DNA.
Effect of oxytocin on ERK1/2 activity.
Phosphorylated ERK1/2 protein relative to total ERK1/2 protein was measured in all cell lines using Bioplex phosphoprotein and total target assays (Bio-Rad, Hercules, CA). For these studies, all cell lines were serum starved for 24 h in medium containing 1 mg/ml bovine serum albumin and then incubated for 10 min with 100 pM oxytocin. Cells were chilled on ice, washed three times with ice-cold PBS, and collected by scraping on ice with 250 μl of lysate buffer. Lysates were clarified by centrifugation for 20 min at 4,500 g at 4°C and stored at −20°C until assayed.
NADPH oxidase activity.
NADPH-dependent superoxide activity was measured by lucigenin-enhanced chemiluminescence (23). Briefly, cells in culture dishes were detached using 0.25% trypsin-EDTA, washed with PBS, and resuspended at 106 cells/ml in PBS-diethylenetriamine-pentaacetic acid (2 mM), and 2.5 × 104cells/well were used for assay in a 96-well black microtiter plate. For cell homogenates, cultured cells were collected by scraping into PBS and lysed by brief sonication (2 × 5-s pulses using a cup horn sonicator probe at ∼50% power). Protein was determined by the BCA Assay.
The reaction was initiated by addition of NADPH (100 μM) and dark-adapted lucigenin (5 μM). Light emission was recorded for 8 min with a luminometer (Centro LB 960; Berthold). Data were expressed as relative light units (RLU) normalized to protein content or cell number and corrected for a sample blank. Each experiment was performed in triplicate.
Control studies using vascular cells and macrophages demonstrated that cellular superoxide generation required NAPDH and was nearly abolished by tiron (5 mM), a nonenzymatic scavenger of superoxide. The NADPH oxidase inhibitor diphenyleneiodonium (10 μM) reduced activity by >80%, whereas the respiratory mitochondrial chain inhibitor rotenone (50 μM) reduced superoxide production by <20%, indicating that the bulk of the superoxide formed was mediated by NADPH oxidase activity (21, 23).
IL-6 assay.
Cells were incubated with LPS (100 ng/ml) or LPS with oxytocin (10 or 100 pM) for 6 h, and then cell-free supernatants were assayed for IL-6 using commercially available reagents (BD Biosciences, San Diego, CA).
Statistical analyses.
Data were presented as means ± SE obtained in at least three separate experiments. Results were compared by paired t-tests or ANOVA with post hoc Tukey tests between experimental and control treatments. All analyses were performed using SPSS for Windows (Chicago, IL). An α-level of 0.05 was required for statistical significance.
For NADPH oxidase activity, a repeated-measures ANOVA was performed with time (min) as the repeated factor and oxytocin dose (control vs. oxytocin dose) as the between-groups factor. The dependent variable was the mean RLU across time (min). Student's paired t-test was used to compare total RLU between experimental and control treatments.
Oxytocin receptor expression.
Expression of oxytocin receptor in endothelial and smooth muscle cells, monocytes, and macrophages was examined. Although endothelial and smooth muscle cells normally exist in the vessel wall, infiltration of monocytes and macrophages occurs in response to inflammatory stimuli, as in atherosclerotic disease. Oxytocin receptor mRNA levels were quantified using real-time PCR (Fig. 1), and results were normalized to expression of 18S. Endothelial and smooth muscle cells express oxytocin receptor mRNA, confirming previous results (33, 37). Smooth muscle cells exhibited 3,600-fold higher mRNA levels relative to endothelial cells. THP-1 monocytes also expressed oxytocin receptor gene. Upon differentiation to macrophages, there was a further 10-fold increase in message levels relative to monocytes.
Oxytocin receptor protein expression was also examined in these cells by immunoblotting (Fig. 2A). There was an immunoreactive band at 67 kDa molecular weight (MW) for endothelial cells, smooth muscle cells, monocytes, and macrophages consistent with the expected MW of the mature glycosylated form of the receptor. There was a fourfold increase of protein expression in THP-1 monocytes and macrophages compared with endothelial cells when normalized for total cell protein; however, there was no difference in expression of the 67-kDa band between the THP-1 monocytes or macrophages. In addition, there was a lower MW immunoreactive band in THP-1 monocytes and macrophages consistent with the molecular size expected for the unglycosylated form of the receptor (∼46 kDa) (17). Upon differentiation of monocytes into macrophages, there was a 15-fold increase in expression of the 46-kDa band.
Cellular localization for oxytocin receptor was examined after subcellular fractionation of THP-1 macrophages (Fig. 2B). Protein expression of oxytocin receptors was also documented in two preparations of isolated human peripheral blood mononuclear cells and in the mouse macrophage cell line RAW 264.7 (data not shown). Upon differential centrifugation, there was a 67-kDa immunoreactive band in the plasma membrane-enriched fraction (100,000 g × 60 min) that was not present in the 10,000-g pellet. The 10,000-g fraction showed an immunoreactive band at the 46-kDa MW that was not apparent in the plasma membrane-enriched fraction. These data indicate that the mature form of the oxytocin receptor is enriched in the plasma membrane fraction. Taken together, our results indicate that oxytocin receptors are expressed in monocytic and macrophage cell lines.
To further establish that the 46-kDa band represented the unglycosylated form of the oxytocin receptor, the membrane pellets that contained predominantly the 67-kDa MW band of THP-1 macrophages were treated with PNGase-F. There was a shift in MW from 67 to 46 kDa after deglycosylation (Fig. 2C), supporting the notion that the 46-kDa band represents the unglycosylated form of the receptor and that glycosylation may be required for plasma membrane localization.
Effect of oxytocin on ERK1/2 phosphorylation.
Oxytocin regulates a variety of physiological functions through its G protein-coupled receptor. Oxytocin receptor activation stimulates the ERK1/2 MAPK pathway in myometrial cells (7, 38) and in oxytocin receptor-transfected Chinese hamster ovary cells (32). To examine oxytocin receptor function, the amount of phosphorylated ERK1/2 protein relative to total ERK1/2 protein was quantified in all cell lines after treatment with oxytocin. Endothelial and smooth muscle cells, THP-1 monocytes, and macrophages treated with 100 pM oxytocin resulted in a significant increase in ERK1/2 phosphorylation compared with control cells (P < 0.05; Table 1). These data suggest that oxytocin is able to activate its receptor in all of the cell types examined.
NADPH oxidase activity.
Oxytocin has been suggested to reduce oxidant stress (12, 13, 28). To explore this possibility, the effect of oxytocin on cellular superoxide production mediated by NADPH oxidase activity was measured. When THP-1 monocytes were incubated with 10 pM oxytocin, there was a 25% decrease in total NADPH superoxide production (P < 0.001 relative to control; Fig. 3A). NADPH oxidase activity was also measured in THP-1 macrophages differentiated with PMA (Fig. 3B). Differentiation of the THP-1 cells increased NADPH-dependent superoxide production fourfold compared with monocytes (compare values in Fig. 3, A and B). Incubation with 10 pM oxytocin decreased total NADPH superoxide production in macrophages by 48% (P < 0.001 relative to control). No further decrease in NADPH-dependent superoxide production was observed at 100 or 1,000 pM oxytocin (data not shown).
The effects of oxytocin on NAPDH oxidase activity in endothelial cells (Fig. 3C) and smooth muscle cells (Fig. 3D) were similarly studied. Incubation with 10 pM oxytocin decreased NADPH-dependent superoxide production by 24% in both aortic endothelial cells (P < 0.01) and smooth muscle cells (P < 0.05) compared with controls.
NADPH-dependent superoxide production increased in TNF-stimulated endothelial cells by 35% (P < 0.05) compared with control (Fig. 4). When endothelial cells were preincubated with 100 pM oxytocin for 1 h and then stimulated with TNFα for 30 min, there was a 36% decrease in NADPH oxidase activity compared with TNF-stimulated endothelial cells (P < 0.05). These data show that oxytocin attenuated TNF-mediated activation of NADPH-dependent superoxide in endothelial cells.
Effect of oxytocin on IL-6 secretion in LPS-stimulated THP-1 macrophages and endothelial cells.
The effect of oxytocin on IL-6 secretion was examined in LPS-stimulated THP-1 macrophages and aortic endothelial cells. THP-1 macrophages and endothelial cells not stimulated with LPS, including those treated with oxytocin alone, showed minimal to no IL-6 secretion (<4 pg/ml; data not shown). Incubation with LPS (100 ng/ml) stimulated IL-6 secretion, and addition of oxytocin resulted in a dose-dependent attenuation of LPS-stimulated IL-6 secretion. There was a 36% decrease in IL-6 secretion when LPS-stimulated macrophages were treated with 10 pM oxytocin (P < 0.01) and a 56% inhibition in cells treated with 100 pM oxytocin (P < 0.001 vs. LPS-stimulated macrophages) (Fig. 5A).
LPS-stimulated endothelial cells were also treated with 100 pM oxytocin, which resulted in a 26% decrease in IL-6 secretion (P < 0.001 vs. LPS-stimulated cells; Fig. 5B). Similar experiments were done in aortic smooth muscle cells; however, these cells exhibit constitutively high levels of IL-6 secretion that was not affected by oxytocin treatment (not shown). Taken together, these data demonstrate that oxytocin attenuates IL-6 secretion from LPS-stimulated THP-1 macrophages and endothelial cells.
The current study demonstrated in an in vitro model that oxytocin has antioxidant and anti-inflammatory effects on vascular cells and THP-1 macrophages. Oxytocin receptors were identified in all cell types examined, and oxytocin decreased NADPH-dependent superoxide production and IL-6 secretion in these cells. By reducing oxidative stress and inflammatory processes in cells present in the vessel wall, these data suggest that oxytocin may have antiatherogenic properties.
Oxytocin receptors on vascular cells, monocytes, and macrophages.
Oxytocin receptor protein and mRNA were identified in aortic endothelial cells and smooth muscle cells, confirming previous data (33, 37). A novel finding was the demonstration of this protein and mRNA in human THP-1 monocytes and macrophages and in two preparations of isolated human peripheral blood monocytes. Oxytocin receptor mRNA was present in aortic endothelial and smooth muscle cells, THP-1 monocytes and macrophages; however, the relative level of oxytocin transcript varied. Using quantitative real-time PCR, there was an approximately 3,600-fold increase in oxytocin receptor mRNA levels in aortic smooth muscle cells compared with endothelial cells. The finding of large differences in mRNA levels between cell types was unexpected considering comparable protein levels among cell types. The discordance between mRNA and protein expression may be due to differential regulation of the receptor at the message or protein level. The relationship between mRNA and protein levels is not well understood and suggests complex regulation of the receptor.
By Western blotting, all cell types examined expressed an immunoreactive band consistent with the expected MW of the mature form of the receptor (18). Additionally, macrophages exhibited a lower (46 kDa) immunoreactive band in THP-1 monocytes and macrophages that was increased 10-fold upon differentiation to macrophages. Studies in uterine tissue have also identified different MW forms of the oxytocin receptor (19). PNGase-F studies support the concept that the lower MW band represents the unglycosylated form of oxytocin receptor. One possibility may be that different glycosylation sites on the receptor may play a role in ligand binding and affinity; however, in vitro studies found that disruption of these glycosylation sites did not affect binding function (17). However, in order for receptors to be functional for targeting their ligands, receptors must be expressed on the cell surface. Differential centrifugation suggests localization of the receptor in different fractions. Further studies are needed to confirm cellular localization and functionality of the different receptor forms.
Oxytocin, NADPH oxidase activity, and IL-6 release.
Oxidative stress and inflammation play an important role in atherosclerosis (3, 22, 29). The current study examined the effects of oxytocin on specific aspects of these disease processes. NADPH oxidase is the major source of superoxide production in the vascular wall (2, 9). A novel finding in the current study was the oxytocin-induced reduction in NADPH oxidase activity in aortic endothelial and smooth muscle cells, THP-1 monocytes, and macrophages. Additionally, oxytocin diminished the induction of NADPH superoxide production by TNFα. A possible explanation for the decreased NADPH oxidase activity was that oxytocin was acting as a superoxide scavenger. It was shown that oxytocin scavenged free radicals and prevented lipid peroxidation at supraphysiological concentrations of oxytocin (0.2–20 μM) (25). Control studies using xanthine-xanthine oxidase to generate superoxide in the presence of up to 1 μM oxytocin had no effect on the measurement of superoxide production, suggesting that oxytocin was not acting as a nonspecific scavenger of superoxide (data not shown).
To assess the putative anti-inflammatory effects of oxytocin, the current study examined IL-6 secretion in oxytocin-treated THP-1 macrophages and endothelial cells. Oxytocin decreased LPS-stimulated IL-6 secretion from macrophages by 56% and endothelial cells by 26%, suggesting an attenuation of inflammatory processes in these cells. This finding is interesting in light of the fact that oxytocin was shown to decrease cytokines in response to LPS in men (5) and that the oxytocin receptor gene contains an IL-6 response element that upregulates the receptor in response to inflammation (16, 30, 31). Because macrophages are found throughout the body, these findings also suggest that oxytocin may have a broader anti-inflammatory function in tissues other than the vasculature.
Social environment, atherosclerosis, and oxytocin.
Previous studies from our laboratory demonstrated that a stable social environment, characterized by increased affiliative social behavior, slowed the progression of atherosclerosis in an animal model (24, 27). In addition, a separate study suggested that social environment differentially modulated inflammatory and oxidative stress mechanisms associated with disease progression (26). Given the extensive literature that relates social behavior to oxytocin and the findings of the current study, it is proposed that elevations in peripheral or local oxytocin as a function of social environment could work directly on vascular cells and macrophages to slow the progression of atherosclerosis. Future studies will examine the mechanisms linking social behavior, oxytocin, and disease.
In summary, oxytocin receptors were identified in cultured aortic endothelial and smooth muscle cells, as well as THP-1 monocytes and macrophages, and the effects of oxytocin on oxidative stress were examined to understand the role of oxytocin in the atherogenic process. The presence of oxytocin receptors were identified on THP-1 monocytes and macrophages for the first time and confirmed in aortic endothelial and aortic smooth muscle cells. NADPH oxidase activity and IL-6 secretion were decreased in vascular cells treated with oxytocin. These novel findings suggest that oxytocin may contribute to an additive effect of decreased superoxide production and decreased IL-6 release that may slow the progression of atherosclerosis.
This study was supported by grants from the National Heart, Lung, and Blood Institute (HL-36588 and HL-04726).
We thank Alessia Fornoni and Silvia Alvarez for their technical assistance.
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
- Copyright © 2008 by American Physiological Society


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The Ruboto team is pleased to announce the release of Ruboto 1.0.2. Ruboto (JRuby on Android) is a platform for developing full stand-alone apps for Android using the Ruby language and libraries. It includes support libraries and generators for creating projects, classes, tests, and more. The complete APIs of Android, Java, and Ruby are available to you using the Ruby language. New in version 1.0.2: This releases updates to JRuby 1.7.10 and improves installation with automated setup of Apache ANT on Windows and Linux. Features: * Issue #447 Use "ruboto setup" to flag and install ant * Issue #564 Update to JRuby 1.7.10 Bugfixes: * Issue #525 Quick Start : "Unfortunately, Browser has stopped" * Issue #565 Odd behaviour when changing orientation with Fragments * Issue #575 Gemfile.lock contains references to gems that are not part of the dependencies Documentation: * Issue #568 Error with tutorial on "Example: Open a web page" Community: * Issue #533 How can I help? (di3z ) * Issue #562 How can I help? (lucasallan) * Issue #563 How can I help? (pedroandrade) * Issue #572 How can I help? (iamrahulroy) You can find a complete list of issues here: * https://github.com/ruboto/ruboto/issues?state=clos... Installation: To use Ruboto, you need to install a Ruby implementation. Then do (possibly as root/administrator) gem install ruboto ruboto setup To create a project do ruboto gen app --package <your.package.name> cd <project directory> ruboto setup To run an emulator for your project cd <project directory> ruboto emulator To run your project cd <project directory> rake install start You can find an introductory tutorial at https://github.com/ruboto/ruboto/wiki If you have any problems or questions, come see us at http://ruboto.org/ Enjoy! -- The Ruboto Team http://ruboto.org/
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realmagick.com The shrine of knowledge.
Vata Vyadhi
- etiological study of vatavyadhi in perspective of modern era
- away from maintaining the Dinacharya Rituchary, Sadvrita etc. which increases the rate of Vatavyadhi. The causes of Vatavyadi which are described.
- www.jbsoweb.com
- A collective clinical approach in the management of 'Vishvachi'
- Nov 23, 2013 The systematic intervention of Siravyadha and Basti therapy gives significant result in Vishvachi. Keywords: Vatavyadhi, Vishvachi, Siravyadha, ...
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- Clinical approach to avarana
- Oct 22, 2012 Vikara, Anubhanda, Gata Vata and Avarana are different pathologies ... Vata Vyadhi can manifest either due to Dhatu Kshaya or. Avarana.
- www.ijrap.net
- concepts of locomotor system diseases in ayurveda and their
- Vatavyadhi the diseases due to derangement/ dysfunction of Vata dosha largely encompass the locomotor or musculoskeletal diseases. Vatavyadhi are ...
- www.euuzlet.hu
- vyadhi and may be categorized as vataja vata vyadhi, samsrishta doshaja vatavyadhi, and avarana vata vyadhi. Margavarana is formed by the component.
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- Ayurveda can Help Combat Big C
- Ayurveda has helped with neurological disorders (vata vyadhi), allergy, skin diseases and many more systems by its holistic approach and yet to shoulder the responsibilities of treating cancer.
- Secret to a Great Leader Lies in a Healthy Life
- The very first hurdle in Patanjali's list of obstacles to success in our endeavours is vyadhi or ill-health. It is not rocket science to keep yourself free from cold, cough and fevers. If Mr Kejriwal is to be effective as a leader ...
- Overcoming Obstacles to Success with Patanjali
- The very first 'obstacle' to achieving your 'goal' is vyadhi or disease. Patanjali's commentators have defined disease in ayurvedic terms to mean an imbalance of the three doshas—vata, pitta and kapha.
- Obesity and Its Management - Gift of Ayurveda
- Moreover, lack of immune power (Vyadhi Asahatva) is mentioned as a common feature as well as serious draw back of Sthaulya so they are frequently prone to secondary diseases. The aim of treating "Sthaulya" is at reducing Vata, Agni and Meda. Neither ...
- Vata Vyadhi: A Handbook for Students of Ayurveda Pendse NM - J
- Dec 26, 2013 Vata Vyadhi: A Handbook for Students of Ayurveda. J Ayurveda Integr Med [serial online] 2013 [cited 2016 Mar 5];4:249-50. Available from: ...
- www.jaim.in
- Among the various maladies of Vata Dosa, Ekangaroga, Khanja, Pangu and Avabahuka resemble the disease of Polio myelitis of modern medicine.
- www.ncbi.nlm.nih.gov
- International Ayurvedic Medical Journal (IAMJ) || VATA VYADHI
- VATA VYADHI (NEUROLOGY) ... VATA VYADHI (NEUROLOGY). >> Cerebrovascular Accident.
- www.iamj.in
- वात व्याधि चिकित्सा विज्ञान: Vata Vyadhi Chikitsa
- वात व्याधि चिकित्सा विज्ञान: Vata Vyadhi Chikitsa. Pages from the book. undefined.
- www.exoticindiaart.com
- Charaka Vatavyadhi Chikitsa 28th Chapter - ayurvedic home
- Jan 3, 2016 28th Chapter of Charaka Chikitsa Sthana is called Vatavyadhi Chikitsa Adhyaya. It deals with treatment for various disorders caused due to ...
- easyayurveda.com
Vata Vyadhi is described in multiple online sources, as addition to our editors' articles, see section below for printable documents, Vata Vyadhi books and related discussion.
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- Research before I buy (86%, 2,523 Votes)
- Impulse buy (14%, 395 Votes)
Total Voters: 2,918
Total Voters: 2,918
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I impulse buy drug store beauty items all of the time, but if I'm going to spend the money on high-end I ALWAYS research (and almost always start my research right here on Temptalia).
I came to makeup later than a lot of my friends…I was 30 when I first started playing with it. Now I have so much makeup I try to only add compliments to my overall collections. I look at swatches online, watch videos, and read reviews on the net before I purchase 95%+ of make up I now add to my stash.
There is some leeway. For example: I like NYX's lipsticks, and they are decently cheap, so if I see a color that I like I'm not going to stress about looking up swatches online.
I actually really enjoy researching products, so I almost research before I buy. It adds to the enjoyment somehow. 🙂
Generally, if it's more than $10 then I research it. I always check comparison swatches to make sure I'm not getting duplicates inadvertently + to check quality and whether it's not just going to be a waste of money.
I didn't always research before I buy, but I have been doing so for the last year. I don't usually research nail polish, I'm kind of an impulse buyer in this instance.
I mainly research buy.. but I do certainly impulse buy with drugstore items or if something cute comes out at Sephora.
A good follow-up question could be "What percentage of your purchases are researched as opposed to impulse buys" as it seems that almost everyone does both!
Almost all of my purchases are researched now, including cheap brands. A few dollars here and there for stuff that ends up being crap adds up to a lot of money FAST!! Most of my impulse buys are usually nail polishes, and I have a few that are streaky tragedies!
I always research before I buy since I mainly purchase high end products, so I do a lot of research online and then I go to the counter to swatch the product and decide if I want it and it suits me. I rarely impulse buy in general anyway not just with makeup but with everything.
I almost never buy cosmetics on impulse, though I crack occasionally – I'm only human! 🙂
Since I'm disabled, I have to buy my cosmetics online, sight unseen, so I read reviews and look at swatches before I buy.
This works surprisingly well; I've only been disappointed a few times, and I have a rather huge collection of products that I love, and that suit me perfectly.
I almost always do a little pre-purchase research now, even on drug store items. Thanks to sources like Temptalia and MUA (both of which are invaluable), there's so much more info out there than in the past.
First I research like mad. Then I sit on it, looking it up periodically to oggle it. Next, I start another wave of researching it, followed by more delaying. The process repeats itself several times, and often times, I never buy the product. I'm a product stalker.
I do a lot of research before I buy products, but I find that when I go to the store to buy them, I generally end up buying something else I see instead.
I research. So when I go into the store I usually amaze the SA LOL. I remember when I was really into MAC I would go in and ask for products by name and the girls there didn't even know what the products were called. It keeps them from trying to sell me products I don't want and it also saves me money.
I research before I buy! Even cheaper drugstore products. I hate wasting money on products that are crap. The only time I impulse buy is if I see something brand new and can't find a review on it.


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25 years of experience
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Arlington, VA 22207 Phone number & directions
A medical license is required for a doctor to practice medicine in a particular state. Requirements vary by state but most require, at a minimum, post-graduate training in the doctor's specific specialty. An NPI number is a national identifier unique to an individual health care provider and is managed by the Center for Medicare & Medicaid Services (CMS).
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This Sacred Heart medal pearl necklace is made with 49 strands of stainless steel, nylon-coated flexible wire. The Swarovski white pearls symbolizing the Immaculate Heart and Swarovski garnet crystals symbolizing the Sacred Heart are 6mm. The 1 1/2" long Sacred Heart/Immaculate Heart medal is solid bronze and is hand cast in the USA from antique pieces. This necklace measures 19 inches, but can be made longer or shorter upon request. One of a kind original creation.


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