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Patent application title: MASKING BACKGROUND FLUORESCENCE AND LUMINESCENCE IN THE OPTICAL ANALYSIS OF BIOMEDICAL ASSAYS
Thomas Krahn (Hagen, DE)
Wolfgang Paffhausen (Leverkusen, DE)
Andreas Schade (Essen, DE)
Martin Bechem (Wuppertal, DE)
Delf Schmidt (Wuppertal, DE)
IPC8 Class: AC12Q106FI
USPC Class:
435 39
Class name: Involving viable micro-organism determining presence or kind of micro-organism; use of selective media quantitative determination
Publication date: 2008-12-25
Patent application number: 20080318270
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In a process for the quantitative optical analysis of fluorescently
labelled biological cells 5, a cell layer on a transparent support at the
bottom 2 of a reaction vessel 1 is in contact with a solution 3
containing the fluorescent dye 4. The sensitivity of analytical detection
can be considerably improved if to the fluorescent dye 4 already present
in addition a masking dye 9, which absorbs the excitation light 6 for the
fluorescent dye 4 and/or its emission light 7, is added to the solution 3
and/or if a separating layer 10 permeable to the solution and absorbing
and/or reflecting the excitation light 6 or the emission light 7 is
applied to the cell layer at the bottom 2. This process can also be used
for improving the sensitivity in the quantitative optical analysis of a
luminescent biological cell layer. The separating layer 10 must in this
case be composed such that it has a high power of reflection for the
luminescent light 11. Analogously, these process principles can also be
used in receptor studies for the masking of the interfering background
radiation in the quantitative optical analysis of fluorescently or
luminescently labelled reaction components. In this case, a receptor
layer 12 at the bottom 2 of a reaction vessel 1 is in contact with a
solution (supernatant 3) in which a fluorescent or luminescent ligand 13
is dissolved. The sensitivity and accuracy of the analytical detection
can be considerably improved here if a masking dye 9 which absorbs the
excitation light 6 for the fluorescent dye and/or its emission light or
(in the case of luminescent ligands) the luminescent light is added to
the supernatant 3. Instead of the masking dye in the solution 3 or
optionally as an additional measure, a separating layer 10 permeable to
the solution 3 and absorbing and/or reflecting the excitation light 6
and/or the emission light or the luminescent light can be applied to the
cell or receptor layer 12 at the bottom 2.
1.-6. (canceled)
7. A composition of matter comprising:a) a reaction vessel having a transparent support at the bottom of said reaction vessel;b) a coherent layer of fluorescently labeled biological cells applied to the transparent support; andc) a solution comprising a masking dye and a fluorescent dye in the reaction vessel, the masking dye absorbing at least one of:i) the excitation energy of the fluorescent dye in the solution; andii) the emission light of the fluorescent dye in the solution, wherein said masking dye does not affect the fluorescence of said fluorescently labeled biological cells.
8. A composition of matter comprising:a) a reaction vessel having a transparent support at the bottom of said reaction vessel;b) a coherent layer of fluorescently labeled biological cells applied to the transparent support; andc) a solution comprising a masking dye and a fluorescent dye in the reaction vessel, the masking dye absorbing at least one of:i) the excitation energy of the fluorescent dye in the solution; andii) the emission light of the fluorescent dye in the solution, wherein said fluorescently labeled biological cells are not stained by said masking dye.
9. A process for the quantitative optical analysis of biological cells labeled with a fluorescent dye, the process comprising the following steps:a) labeling a coherent layer of biological cells on a transparent support at the bottom of a reaction vessel with a fluorescent dye in a solution;b) shining excitation energy through the transparent support to cause the biological cells labeled with the fluorescent dye to exhibit fluorescence in the presence of a solution comprising the fluorescent dye and a masking dye absorbing at least one of:i) the excitation energy of the fluorescent dye in the solution; andii) the emission light of the fluorescent dye in the solution; andc) detecting the fluorescence of the biological cells labeled with the fluorescent dye through the transparent support.
[0001]The invention originates from a process for the quantitative optical
analysis of fluorescently labelled biological cells which are in contact
with a fluorescent dye solution or of luminescent cells which are applied
to a transparent support at the bottom of a reaction vessel in the form
of a coherent cell layer, or alternatively of fluorescently or
luminescently labelled reaction components in a solution in which a
fluorescent or luminescent ligand is dissolved, the solution being in
contact with a receptor layer, which is specific for this ligand and
situated on the transparent support at the bottom of the reaction vessel,
whose fluorescent or luminescent radiation, which is characteristic of
the receptor-ligand binding, is detected and analysed through the
transparent bottom.
[0002]A problem in fluorescence measurement in biomedical assays is often that the fluorescence changes correlated with the biological cell action are small compared with the non-specific background fluorescence. As a result, the resolving power is greatly restricted. Conventional commercial measuring systems (fluorescence readers, Dynatech or SLT), cannot solve the problem, because owing to their optical measuring arrangement (excitation from `above` through the fluorescent liquid column of the supernatant) the signal can barely be detected in comparison with the background. Apparatuses of newer construction (Labsystems), which illuminate the cells from the back through the transparent support of the reaction vessel, do have the advantage that on entry of the excitation light the cells are excited to fluorescence. Since the excitation light, however, enters further into the supernatant, which is also fluorescent, the fact that the non-specific background signal adulterates the cell signal cannot be avoided. Even very complicated measuring systems (NovelTech, FLIPR: Fluorescence Imaging Plate Reader) are only able to decrease this background fluorescence using a special laser illumination geometry (excitation below about 45°). The reason for the failure of all problem-solving experiments on the measuring geometry is the fact that the actual cause of the background fluorescence cannot be decisively influenced hereby.
[0003]In the receptor binding studies carried out until now using fluorescently or luminescently labelled ligands, the labelled and unbound fraction in each case must be removed by processes like washing. Many coatings, however, are sensitive to these washing steps. Moreover, the removal of the unbound ligand is associated with a considerable outlay. The direct measurement of the receptor-ligand association or dissociation is not possible in this process.
[0004]The invention is based on the object of improving the sensitivity of the optical analysis of fluorescently labelled or luminescent cells in a cellular assay in order to be able to measure, for example, membrane potential changes which are as low as possible on the basis of fluorescence changes of potential-sensitive dyes. In this case, the sensitivity of the measuring system should be so high that potential changes of below 5 mV can be detected at least qualitatively. In the case of luminescent cells, an increase in the detection of the luminescence signal should be achieved. Moreover, the method should be suitable for screening with a high sample throughput.
[0005]The invention is furthermore based on the object of simplifying receptor binding studies based on fluorescently or luminescently labelled ligands or receptors and making possible continuous measurement of the receptor binding interaction (kinetics). Owing to the reduction in the process steps necessary, this method should be particularly suitable for screening with a high throughput and for diagnostic applications.
[0006]It was only possible to achieve the required high resolution with low membrane potential changes after it was possible to eliminate the cause of the interfering overlapping of the non-specific background fluorescence and the specific fluoresence of the cells. The process according to the invention developed for this purpose is based on the fundamentally new idea of masking the excitation energy and the fluorescence not originating from the biological object. To do this, in addition to the fluorescent dye, a further dye is added which completely absorbs the excitation light of the fluorescent dye and/or its emission light without affecting the fluorescence of the cells. By means of this absorption, the non-specific background signal is masked and the useful cell signal can be detected with a resolution which was previously not possible.
[0007]An alternative solution which is within the scope of the invention is that a separating layer, which is permeable to the solution and which absorbs and/or reflects the excitation light for the fluorescent dye and/or its emission light without adversely affecting the cell properties, is applied to the cell layer. At the same time, the thickness of the separating layer is selected such that fluorescence is no longer detectable in the dissolving mixture with the fluorescent dye but without the cells.
[0008]A further variant of the invention is that the method of the separating layer according to the invention is also used for increasing the sensitivity in the quantitative optical analysis of luminescent (luminous) biological cells which are applied to a transparent support in the form of a coherent cell layer. For this purpose, the optical properties of the separating layer permeable to the solution are selected such that it reflects the luminescent light as strongly as possible without adversely affecting the cell properties. In this manner, it is possible to increase the luminescence intensity and thus the measured effect considerably.
[0009]The process according to the invention can be used in a completely analogous manner for the quantitative optical analysis of fluorescently or luminescently labelled reaction components in a reaction vessel filled with a solution, the fluorescent or luminescent ligand being present in dissolved form and the solution being in contact with a receptor layer which is specific for this ligand, applied to a transparent support at the bottom of the reaction vessel or deposited thereon, whose fluorescent or luminescent radiation, which is characteristic for receptor-ligand binding, is detected and analysed through the transparent bottom. In this case, the solution according to the invention of the object described above is based on the fact that the free ligand which is in the supernatant, i.e. in solution, and its non-specific fluorescence or luminescence is masked by an additional dye and/or by a diffusely absorbing or reflecting separating layer and thus the cause of the interfering overlapping of the non-specific background fluorescence and the specific fluorescence of the ligand in the solution is eliminated. Since the non-bound ligand is masked in this manner, the measured fluorescence or luminescence is a direct measure of the ligand-receptor interaction. It can be measured directly in this process with time resolution.
[0010]In receptor studies, in analogy to the process described above, the invention thus relates to a process variant in which a masking dye is added to the solution and/or a separating layer permeable to the solution is applied to the receptor layer, the optical properties of the masking dye and/or of the separating layer being selected such that the excitation light for the fluorescent dye of the ligand present in the solution and/or its emission light or its luminescent light is absorbed by the solution or the separating layer or reflected at the separating layer. In this case, the thickness of the separating layer is selected such that fluorescence is no longer detectable in the dissolving mixture with the fluorescent dye, but without the receptor layer.
[0011]The separating layer preferably consists of polymeric latex beads (e.g. polystyrene, polyurethane, butadiene, acrylonitrile). The latex beads can also be dyed with a masking dye, which in this case must have an adequately high polymer dyeing capacity.
[0012]In the first-mentioned process, the masking dye should be as well distributed as possible in the solution which also contains the fluorescent dye in dissolved form. Since, as a rule, the solvent is water, a masking dye is expediently employed which possesses good water solubility (>2 g/ml) and has no cytotoxic side effects.
[0013]According to a further development of the invention, after the replacement of the supernatant containing a fluorescent dye by a fluorescent dye-free solution, a further masking dye is added which suppresses a non-specific fluorescence on the reaction vessel wall.
[0014]The following advantages are achieved using the invention:
[0015]The new process described is not tied to a certain measuring system, but can be used, because it is not a specifically technical solution, by many commercially available apparatuses. These include virtually all fluorescence readers which can illuminate and also measure transparent reaction vessels, e.g. microtitre plates, from the bottom. With a very low outlay (minimal additional, costs only for the special absorption dyes), it is possible for the first time by this means to advance in a resolving area, e.g. in the measurement of potential changes in cell membranes by measurement of the change in fluorescence of potential-sensitive fluorescent dyes, which was unachieved until now. For the first time it is possible even in the case of very low changes to carry out a direct comparison of the results from various reaction vessels (e.g. various wells in a microtitre plate), such that the complicated procedure of the determination of the relative change in a reaction vessel can be dispensed with. As a result the number of measurements to be determined, e.g. for kinetic measurements, decreases. The outlay in terms of time for a measuring programme is markedly reduced and the possibility created of obtaining identical results by a simple individual measurement (e.g. end point determination) with the use of reference to a separate control batch. The uniformity of the biological batch required in this case (e.g. homogeneous cell layer) is generally afforded, for example, for microtitre plates.
[0016]Surprisingly, the use of various water-soluble dyes and also their mixtures in the very different cells tested showed no negative effect on the physiology of the cells (e.g. reaction of the cells in comparison with electrophysiological measurements such as whole-cell patch-clamp, or effects of the pharmaceuticals investigated). The use of undissolved dye pigments or inorganic finely divided particles was also surprisingly well tolerated by the biological objects.
[0017]As a result of the simple process described for masking the background fluorescence in quantitative fluorescence measurement in biomedical assays, connected with an increase in the sensitivity, e.g. when using potential-sensitive fluorescent dyes, and the adaptability of this process, e.g. to microtitre plates as reaction vessels, the use of such measuring techniques will significantly simplify high-throughput screening, especially as no increased technical expenditure is necessary for the realization of the advantages outlined, but existing commercial measuring apparatuses are sufficient for this purpose. In receptor-ligand studies, the advantage essential to the invention is that, on account of the masking of the non-specific fluorescence or luminescence, it is no longer necessary to remove the unbound fraction of the ligands. As a result, the test procedures are considerably simplified, damage to and destruction of the sensitive coatings or of the biological objects such as, for example, cells are avoided and the sensitivity and thus also the accuracy of the measurement are improved. As a result of the use of microparticles, the utilizable surface area for the coating of fluorescently or luminescently labelled ligands can be significantly increased. By means of suitable measures, e.g. relatively high specific density or the use of magnetizable particles, the settlement and concentration of the microparticles on the transparent support can be achieved. In this case too, the fluorescence or luminescence of the unbound ligands in the supernatant is effectively suppressed by the masking.
[0018]Since the interaction between the ligands and the receptor must not be interrupted by the removal of the unbound fraction, a continuous measurement of the interaction between ligand and receptor (kinetics) can be carried out in this manner even in an individual reaction batch.
[0019]The invention is explained in greater detail below with the aid of working examples and drawings, wherein
[0020]FIG. 1 shows a reaction vessel for a fluorescence assay according to the prior art
[0021]FIG. 2 shows the suppression of the background fluorescence in a fluorescence assay with a masking dye in the supernatant
[0022]FIG. 3 shows the spectral excitation and emission for a dispersion dye and the spectral absorption of the masking dye
[0023]FIG. 4 shows the site-dependent cell fluorescence without masking dye
[0024]FIG. 5 shows the site-dependent cell fluorescence with masking dye
[0025]FIG. 6 shows the suppression of the background fluorescence in a fluorescence assay with the aid of a separating layer
[0026]FIG. 7 shows the amplification of the luminescence by back-reflection from a separating layer
[0027]FIG. 8 shows the wall fluorescence in a fluorescence assay according to the prior art
[0028]FIG. 9 shows the suppression of the wall fluorescence in a fluorescence assay with the aid of a masking dye and
[0029]FIG. 10 shows the suppression of the background fluorescence or luminescence in a fluorescence or luminescence assay for the investigation of receptor-ligand binding with the aid of a masking dye in the supernatant
[0030]FIG. 1 shows a reaction vessel 1 for a fluorescence assay with a transparent bottom 2. A fluorescent dye solution 3, in which the fluorescent dye molecules 4 are indicated schematically, is found in the reaction vessel 1. The solution 3 is also designated as the supernatant. The biological cells to be investigated are arranged on a transparent support on the transparent bottom 2. Light (excitation light) 6 is shone through the bottom 2 in order to excite the cells 5 to fluorescence. A background fluorescence radiation 8, which originates from the likewise excited fluorescent dye molecules 4 in the supernatant 3, overlaps the fluorescent light 7 emitted by the cells 5. Only the fluorescent light 7, however, is decisive for the bioanalytical investigation and analysis of the cells 5. Since, however, in all known fluorescence analysis apparatuses the background fluorescence 8 is additionally determined, small fluorescence differences of the cells 5 are lost in the strong background fluorescence 8, which leads to a marked sensitivity loss.
[0031]This disadvantage can be avoided by the procedure according to the invention of FIG. 2 by suppressing the background fluorescence by a masking dye in the supernatant 3. The background fluorescence 8 present in FIG. 1 is completely absorbed in the supernatant according to FIG. 2. The masking dye (schematically designated by 9) added to the supernatant 3 can either be present in dissolved form or in finely divided disperse phase (colour-pigmented systems). Preferably, however, soluble dyes are employed, because in this case the addition can be carried out particularly simply with the aid of a pipette and because, in contrast to a pigment system, the physical effects of particle size distribution and of sedimentation processes and layer thickness inhomogeneities do not have to be taken into account.
[0032]The following demands are made on the properties of a dye of this type: [0033]when using a soluble absorption dye, good water solubility for use in biological assays [0034]no membrane permeability of the dye in order to avoid staining of the cells [0035]high specific absorption in the excitation and/or emission wavelength range of the fluorescent dye [0036]no toxic side effects (avoidance of cell damage)
[0037]A solubility of >2 mg/ml is regarded as good water solubility. The cell toxicity can be determined with the aid of known test procedures (e.g. cytotoxicity test). FIG. 3 shows the optical (spectral) properties of a fluorescent dye and of a masking dye in a graph. Curve A shows the spectral distribution of the excitation light, curve B the spectral distribution of the emitted fluorescent light for the commercially available dispersion fluorescent dye bis(1,3-dibutylbarbituric acid)trimethaneoxonol (Dibac4(3)) and curve C the spectral transmission (absorption spectrum) of the masking dye used (Brilliant Black BN, C.I. 28440, Food Black 1, e.g. Sigma B-8384). It is recognized that the masking dye is almost completely absorbed in the wavelength range of the excitation and emission of the fluorescent dye.
[0038]Contrast enhancement or increase in sensitivity can be even better understood with the aid of FIGS. 4 and 5. To demonstrate the action of the masking dye on the non-specific background fluorescence, two video recordings of the same image section were made before and after adding 100 mg/ml of the soluble masking dye Brilliant Black in the presence of the potential-sensitive fluorescent dye Dibac4(3) (5 mM). Both times, the same video line was assessed by image analysis and the two fluorescence intensity profiles shown over the identical sections in the reaction vessel. The region Z in this case corresponds to the region in which the cell layer, i.e. the biological sample, is found, while right of this in the zone U, to the greatest part, the fluorescence signal originating from the supernatant is measured. The measuring range of the recording system (8 bit) is between 0 (black) and 255 (white). For the unmasked recording, a contrast ratio of about 1:3.6 results (intensity ratio of the darkest and lightest image portions) and in the case of the masked recording a contrast ratio of about 1:14.4. This corresponds to a contrast increase by a factor of 4.
[0039]According to FIG. 6, an alternative possibility of improving the ratio of useful to background signal is covering of the cell layer with a finely divided optical separating layer 10. The separating layer 10 expediently consists of a finely divided inorganic white pigment, such as, for example TiO2 or Al2O3. By means of this, not only the background fluorescence radiation from the supernatant 3 is screened off, but also the measurable quantity of cell fluorescence is increased by reflection from the inorganic particles.
[0040]Alternatively, the separating layer can consist of polymeric latex beads having a diameter preferably in the range from 200 nm to 5 mm. Suitable polymers are, for example, polystyrene, polyurethane, butadiene, acrylonitrile. The latex beads can also be dyed with a suitable masking dye to which the same criteria apply as for the absorption dye added to the solution (see above). A suitable class of dye is, for example, ®Resoline.
[0041]In luminescence assays (luminous cells), the requirements fundamentally consist of detecting the specific very low light intensity of a biological cell with high sensitivity. By applying a reflecting separating layer 10 according to FIG. 7, it is possible, analogously to the method for suppression of the background fluorescence (according to FIG. 6), to increase the luminescence signal of the biological cells. In this connection, radiated fractions 11 of the undirected luminescent light are reflected in the direction of the detector and thus increase the specific measuring signal.
[0042]In a large number of other fluorescent test procedures on biological cells, it is possible, in contrast to dispersion dyes, to remove the fluorescent dye from the supernatant after staining of the cells by solution exchange. The fluorescent dye FURA2-AM is cleaved, for example, into the free dye after penetrating into the cell and in this case loses its cell membrane permeability. As a result, a concentration of the impermeable fluorescent dye in the cell occurs. In this case, the fluorescent supernatant 3 can be replaced by a fluorescent dye-free solution 3a without changing the specific cell fluorescence. The non-specific background fluorescence of the supernatant is removed in this manner. FURA2-AM, however, stains reaction vessels persistently (wall fluorescence) and thus produces another non-specific fluorescence signal which is comparable with the background fluorescence of dispersion dyes. This situation is shown in FIG. 8. In this case, the background fluorescence radiation 8 is attributed to the fluorescent dye molecules 4 adhering to the vessel walls. By incorporation of masking dyes in the fluorescent dye-free supernatant 3a, this non-specific fluorescence signal can also be completely suppressed.
[0043]In FIG. 10, a working example analogous to FIG. 2 is additionally shown in which the biological layer applied to a transparent support at the bottom 2 of the reaction vessel 1 consists of receptors 12 which enter into a specific bond with the fluorescently or luminescently labelled ligand 13 present in the supernatant (solution) 3. The bound ligands are designated here by 14. In the case of the unlabelled solution, the primary light 6 shone through the bottom 2 excites the fluorescently labelled ligands 13 and 14 to fluorescence. In the case of luminescently labelled ligands, the primary light 6 is inapplicable. Analogously to the implementation according to FIG. 2, a masking dye which takes care that the fluorescent or luminescent radiation emitted from the unbound ligands 13 is completely absorbed in the solution is in turn added to the solution 3. The fluorescent or luminescent radiation 15 determined at the bottom 2, i.e. the measured effect, is therefore very predominantly attributed to the ligands 14 bound to the receptors 12 and is not adulterated by the background radiation of the unbound ligands 13 in the solution 3. The measuring signal is therefore a direct measure of the strength of the ligand-receptor binding. In this case, the layer thickness of the receptor layer is in the nm range, while the dimensions of the supernatant situated above it are in the order of magnitude of several mm.
[0044]According to FIGS. 6 and 7, the separating layer 10 permeable to the solution can be employed in the investigation of ligand-receptor binding in an entirely analogous manner to the masking or suppression of the background fluorescence or luminescence. In this case, the interfering background fluorescence or luminescence is screened off by the separating layer 10 and in the case of luminescent ligands radiated fractions 11 of the luminescent light originating from bound ligands are reflected in the direction towards the reflector and thus the useful signal is increased.
[0045]The non-fluorescent or luminescent reaction component to be assessed with respect to its binding strength in classical pharmacological receptor binding studies was not shown here for reasons of clarity.
Patent applications by Andreas Schade, Essen DE
Patent applications by Delf Schmidt, Wuppertal DE
Patent applications by Martin Bechem, Wuppertal DE
Patent applications by Thomas Krahn, Hagen DE
Patent applications by BAYER HEALTHCARE AG
Patent applications in class Quantitative determination
Patent applications in all subclasses Quantitative determination
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Pursuant to a congressional request, GAO conducted a comprehensive review of the report prepared by Donald T. Bucklin of Squire, Sanders & Dempsey for the Department of Housing and Urban Development (HUD), focusing on identifying inaccurate statements found in the Bucklin Report. The Bucklin Report was prepared to assist HUD in its defense against an equal employment opportunity complaint filed by HUD's Deputy Assistant Inspector General.
GAO noted that: (1) the Bucklin Report contained several factual inaccuracies; and (2) GAO provided the correct fact for each inaccurate statement cited in the report.
Aug 3, 2016
FACE Recognition Technology:FBI Should Better Ensure Privacy and Accuracy [Reissued on August 3, 2016]GAO-16-267: Published: May 16, 2016. Publicly Released: Jun 15, 2016.
Aug 1, 2016
Firearms Data:ATF Did Not Always Comply with the Appropriations Act Restriction and Should Better Adhere to Its PoliciesGAO-16-552: Published: Jun 30, 2016. Publicly Released: Aug 1, 2016.
Jul 14, 2016
DOJ Grants Management:Justice Has Made Progress Addressing GAO RecommendationsGAO-16-806T: Published: Jul 14, 2016. Publicly Released: Jul 14, 2016.
Jul 5, 2016
Gun Control:Analyzing Available Data Could Help Improve Background Checks Involving Domestic Violence RecordsGAO-16-483: Published: Jul 5, 2016. Publicly Released: Jul 5, 2016.
Jun 30, 2016
Program Integrity:Views on the Use of Commercial Data Services to Help Identify Fraud and Improper PaymentsGAO-16-624: Published: Jun 30, 2016. Publicly Released: Jun 30, 2016.
Jun 28, 2016
Human Trafficking:Agencies Have Taken Steps to Assess Prevalence, Address Victim Issues, and Avoid Grant DuplicationGAO-16-555: Published: Jun 28, 2016. Publicly Released: Jun 28, 2016.
Human Trafficking:Implementation of Related Statutory Provisions, Law Enforcement Efforts, and Grant FundingGAO-16-748T: Published: Jun 28, 2016. Publicly Released: Jun 28, 2016.
Jun 23, 2016
Federal Prison System:Justice Has Used Alternatives to Incarceration, But Could Better Measure Program OutcomesGAO-16-516: Published: Jun 23, 2016. Publicly Released: Jun 23, 2016.
Jun 22, 2016
Drug Enforcement Administration:Additional Actions Needed to Address Prior GAO RecommendationsGAO-16-737T: Published: Jun 22, 2016. Publicly Released: Jun 22, 2016.
Jun 21, 2016
Controlled Substances:DEA Should Take Additional Actions to Reduce Risks in Monitoring the Continued Eligibility of Its RegistrantsGAO-16-310: Published: May 26, 2016. Publicly Released: Jun 21, 2016.
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Statement Earrings
Zoe wears Dana Rebecca and Repossi (similar here)
Rihanna wears Dior (similar here)
Cara wears Chanel (similar here)
Nicole wears House of Harlow 1960 (similar here)
Kate wears delicate gold earrings
Emma wears Delfina Delettrez (similar here)
Images: Harper's Bazaar · CR Fashion Book
Earrings are my latest jewellery of choice. I'm sure you know this if you have seen this post. Love the way Emma and the model rocks a single statement earring.
Get the look:
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This review is from: HP LA2205WG LA2205WG 22" 5ms Widescreen LCD Monitor 250 cd/m2 1,000:1
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Beaverhead-Deerlodge National Forest
Contact Information
Beaverhead-Deerlodge National Forest
420 Barrett St.
Dillon, MT 59725-3572
Phone number:
How to contact all Beaverhead-Deerlodge National Forest offices
Contact Us
Where is this Forest?
You can order forest maps through the USDA Forest Service's National Forest Store. NEW! - The U.S. Forest Service now offers access to a variety of visitor maps for people using Android and iOS devices. For more information on the mobile app and access to digital maps, click here.
The Beaverhead-Deerlodge National Forest maps are also available for sale at the Supervisor's Office and all District Offices for $10 each. View our map index to locate the map you need.
Horse Sense - Packing Lightly
Personal Use Firewood Gathering
"Who Fixes the Fence?" - Information for Landowners Living Next to the National Forest
Recreational Drone Tips - For responsible hobby or recreational use of Unammed Aircraft Systems (UAS) or "Drones"
Is the Water Safe to Drink?
Hunting Season
Harvesting Mushrooms Guide
2015 Recreation Opportunities on the Beaverhead-Deerlodge
Dillon, Madison, Wisdom, and Wise River Ranger Districts (South end of the Forest)
Butte, Jefferson, and Pintler Ranger Districts (North end of the Forest)
2014 Lake and Fishing Directory
2012 Pioneer Scenic Byway ROG - a travel guide for the 49 mile Pioneer Mountains National Scenic Byway
Recreational Prospecting Guide
Landscape Rock Collection Information
Unpatented Mining Claims
Chief Joseph Pass Cross-Country Ski Trails, groomed
Chief Joseph Area Cross-Country Ski Touring Trails, ungroomed
Georgetown Lake Cross Country Ski Trails 2013
Pioneer Mountains Snowmobile Routes Map
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"Experts nearly all recommend Vote Smart."
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Ron Seiford's Biography
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Full Name:Ron Seiford
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Birth Place:St. Paul, Minnesota
- Bachelors, Arizona State University
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- 1/2 cup sesame paste
- 1 cup water
- Juice of 2 lemons
- 4 garlic cloves, chopped
- 1 teaspoon ground cumin
- Salt, to taste
- Place sesame paste in the bowl of a food processor with water, lemon juice, garlic cloves, and cumin. Pulse to combine, then process until the mixture has the consistency of thick cream. Transfer to a bowl and add salt to taste.
- Tehina will keep for at least a week, tightly covered in the refrigerator.
Memories of a Lost Egpyt: Reminiscences and Recipes /n
/nClarkson N. Potter
Nutritional Info
- Calories111
- Carbohydrates6 g(2%)
- Fat9 g(14%)
- Protein4 g(7%)
- Saturated Fat1 g(6%)
- Sodium432 mg(18%)
- Polyunsaturated Fat4 g
- Fiber2 g(9%)
- Monounsaturated Fat3 g


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Jefferson runway bids opened
STEUBENVILLE — Bids were opened Thursday by the Jefferson County commissioners for the runway extension project at the Jefferson County Airpark.
But the opening was delayed about 30 minutes because the engineer for the project didn't have an exact estimate for the work. The county received $1.5 million in state grants for the project, with the county contributing $500,000.
An engineer with Michael Baker Jr. Inc., the engineering firm, said there had been numerous changes in the project to ensure it comes in under budget. After a delay, the engineer and representatives of the county airport authority returned with an estimate of $2.17 million.
Commissioner David Maple said he was concerned with not having an estimate because commissioners are forced to reject bids if the bids are more than 10 percent over the engineer's estimate.
Six companies bid on either electrical, earthwork or paving. The airport authority now will review the bids and make a recommendation.
Commissioners also heard complaints from residents in the Crestview-Belvedere area about reconstruction work not being properly completed by Rudzik Excavating after the installation of sewers.
The residents said topsoil and grass seeding wasn't properly done, and yards are in worse condition after the job. The county had an agreement with residents that the yards would be restored to the same condition after construction.
Commissioner Tom Gentile said there appears to be a reoccuring theme with Rudzik — the company did a good job in installing the sewers but aren't doing as good of a job in restoration of yards.
Shannan Gosbin, county water and sewer department director, said there are about 10 property owners making the poor restoration claims out of 430 property owners in the project area.
Anthony Recinella of 225 Hilda Circle, Bloomingdale, said there are many more residents upset over the yard restorations but believe nothing will be done if they complain.
County Commissioner Thomas Graham said the commissioners will continue to investigate the complaints.
"We aren't going to let this sit," he said.
Commissioners said they won't pay the remaining 10 percent owed on the contract until the restoration issues are resolved by Rudzik.
Commissioners also met in executive session with six employees of the Jefferson County Job and Family Services, Children Services Division, concerning complaints about Elizabeth Ferron, agency director.
Ferron wasnt present at the commissioners' meeting on Thursday.
Commissioners also discussed a verbal contract with Miller Septic, which exchanges pumping services at county sewage treatment plants and in other areas of the system in exchange for dumping septic waste at the countys sewage treatment plants. The verbal agreement dates back to the late 1990s.
Commissioners received a letter from another septic pumping business wanting to dump septic waste at the county's sewage treatment plant but was told the county's water and sewer department wont allow it.
Only Weirton's sewage treatment plant allows septic haulers to dump waste. Other sewage treatment plants in Steubenville, Toronto, Brilliant, Wintersville and Mingo Junction do not allow the practice, according to Gosbin. She noted the county's sewage treatment plants currently operate above permitted capacity.
Gosbin said Miller Septic has served the county well over the years under the verbal agreement.
Graham noted the board doesn't operate under verbal agreements.
Commissioners also:
- Agreed to advertise on behalf of the county's regional planning commission for Community Development Block Grant sidewalk replacement projects in Adena, Rayland and Richmond and a paving project in Richmond.
- Agreed to provide $5,719 in funding to the county fair board to purchase a brush hog from Kuester Implement to cut brush on county fairgrounds. The fair board obtained five quotes for the equipment.
- Proclaimed Saturday as World Elder Abuse Awareness Day on behalf of the county's Job and Family Services Department.
- Entered into five contracts on behalf of the county Job and Family Services Department to house children with special needs at facilities outside the county. The per-day cost ranges from $99 to $360.
- Entered into a contract with the Steubenville Health Department at a cost of $3,244 to inspect 17 home day care providers in the city. County job and family services inspects home day care providers outside the city.
- Entered into a contract between county juvenile court and the Ohio Department of Job and Family Services for court services to children meeting income guidelines. The two-year agreement allows the county juvenile court to recoup up to $1.5 million a year for the services. Joseph Colabella, juvenile court administrator, said the court receives between $600,000 and $1 million a year.


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Town supervisors in both Fulton and Montgomery counties have filed candidacy petition challenges against their opponents, and election officials have already ruled on the Montgomery County challenge. Palatine Supervisor Brian Sweet filed a petition challenge July 14 against fellow Republican Charles Dillenbeck, at least partly on the grounds that Dillenbeck does not reside in the town of Palatine. "He doesn't live in my town, nor has he ever lived in my town," Sweet said of ...
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An asinine adventure through the world of high fantasy.4.47 / 5.00 2,336 Views
Nothing To Fear
Super villainy at its worst ...4.17 / 5.00 3,407 Views
The Ascent of Alfred
Make Alfred Great Again4.10 / 5.00 11,869 Views
so short?
damn too short =[
i want more.. :<
I forgive your comment. :P If I would have put credits at the beginning, it would be the same length as Episode #2!
Nice Dude.
The animation in this series is very playful and well done. It could honestly be a TV show.
I'm so glad your gonna let me be apart of this series. Now bring on episode 4!!
Thanks! I'm happy to see how much I can get away with, with tons of tweens. :) And I'm definitely glad to have as a part! Episode #4 GO!
That was a nice animation, but i really liked more your last Tywo At Large episodes.
Well. It was nice.
This is just a taste of what's to come, glad you liked it.
Another great Tywo movie.
Everything in this movie is great. I agree with you, it is not a full Tywo episode. "Tywo short" sounds better for me.
I can't let this go by with only 2 reviews...
Like always anything that you create just seems to be a masterpiece. I wish I could be half as good as you are with animation. Hell if I was a quarter as good as you were then I'd still be a popular artist on this website. Oh well, I just don't have the god given ability that you have to draw and animate.
~ Animation/Graphics ~
Not bad.. not bad for a Sayre kid that is. :P
Ha, amazing like always. Very smooth all around and you do a great job of paying attention to details such as facial features. Great job drawing and animating all around.
~ Story/Content ~
Ha, a nice little joke within the submission with the whole snow thing. I got a good laugh at the kid getting so excited to go shovel snow. Then the fires of hell came down and added humor to the submission.
The tape recording at the end was a nice joke. A bit predictable, but nonetheless a decent joke.
~ Audio ~
Pretty good voice acting and pretty good sound effects. Also a good choice of music. Good job all around here. You couldn't use me for a guest voice actor sometime could you? :P
~ Overall ~
Like always keep up the good work. You're great at it.
Your review wasn't bad... For a Canton kid. XP Thanks though, I'm surprised I was able to get it done, just 12 hours before, half the movie was still incomplete! You wanna do a voice, really?


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After a change of career to education, Dr. Debbie Bass did some substitute teaching before becoming a Busbee Elementary School teacher.
She later took a position at Wagener-Salley before moving to A.L. Corbett Middle School as assistant principal and then principal before retiring.
In March, she was invited to serve as interim principal at New Ellenton Middle School – ending that assignment last month with the arrival of new principal Shunte Dugar.
Bass was impressed with the level of commitment to the students from the faculty, staff, community and parents.
"I was glad to be able to contribute to the school and work with the School District again," Bass said via email. "It was certainly a wonderful place to lend my expertise. I am glad I had the opportunity to step out of retirement for a short period to support the efforts of the district."
Bass also had the chance to recognize students and community residents for their accomplishments before school ended.
• New Ellenton community volunteers were recognized for the contributions to the school – among them Mayor Vernon Dunbar, Rachel Davenport, Marian Webb, Alex Osbon, Cindy Boles and Helen Green. They represented the Parent-Teacher Organization, the School Improvement Council, the City of New Ellenton, Christ Central and area churches.
• The Junior Beta Club inducted 21 new members at a ceremony. The current members participated in a candlelighting – Tamia Valentine, Peyton McMurray, Keyera Odom, Shikorian Corbitt, Amaya Odom, Madeline Walls and adviser Bonnie McNeil.
The new inductees are Jalyn Brooks, Jayda Bush, Krysta Byars, Logan Channel, Jaden Cook, Larrissa Fiquris, Jessica Frias, Zykeira Green, Cameron Jackson, Zion Krauss, Jameshia Oakman, Destiny Robertson, Shicago Robertson, Lucero Rodriquez, Viviana Romero, Cierra Smith, Sydney Thompson, Jaliya Toomer, Madeline Walls, Justin Walker and Taylor Young.
• The New Ellenton stock market team received third place at the 24th S.C. Economics Awards Luncheon, held at the Brookland Conference Center in West Columbia. McNeil also serves as that team adviser.
Senior writer Rob Novit is the Aiken Standard's education reporter and has been with the newspaper since September 2001. He is a native of Walterboro and majored in journalism at the University of Georgia.
SUBMITTED PHOTO New Ellenton Middle School's award-winning Stockmarket Team are, from left, Cierra Smith, Vanessa Campbell, adviser Bonnie McNeill and Marihelen Burton.×
SUBMITTED PHOTO Students, joined by Dr. Debbie Bass, right,then-interim principal, participate in New Ellenton Middle School's Junior Beta Club candlelighting ceremony. Pictured, from left, are Peyton McMurray, Keyera Odom, Shikorian Corbitt and Amaya Odom.×


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- Home
- Pregnancy
Pregnancy topics
years NCT has been campaigning as the voice for parents on the issues they care about
- Birth
Birth topics
branches across the UK run thousands of events all year round
- Parenting
Parenting topics
NCT practitioners support parents to find information they need to prepare for their new life
- Branches & events
Parenting topics
NCT practitioners support parents to find information they need to prepare for their new life
- Courses
Parenting topics
NCT practitioners support parents to find information they need to prepare for their new life
Welcome to NCT Westminster
We cover the following areas: Pimlico, Mayfair, Soho, Paddington and Maida Vale. Our branch was created in the summer of 2009.
Upcoming Events:
Westminster NCT are delighted to announce the date for their next Nearly New Sale as Saturday 31st May 2014, 10.00am – 12.00pm, at Grosvenor Hall, Vincent Street, SW1P 4EP. Entry fee £2 per person.
The first 100 people will receive a goody bag and NCT members get priority access at 10am so please make sure you bring your membership card to show. Non-members will be able to enter from 10.15am.
If you need a clear-out, and would like to book a table for just £10 to sell your second-hand items, please contact Julia on the below email address for more info. You are welcome to go halves on a table with a friend! Just £5 for half a table, and you'll get rid of that clutter and be able to treat yourselves to a curry and a bottle of wine!!
A lot of people do manage to make £100s, so it's really worth it!
Big Coffee Morning - Every last Thursday of the month at Grosvenor Hall, Vincent Street from 10am-12pm.
Come along for a coffee and a chat with only local mums whilst the little ones can have a play and let off some steam! We usually finish with some singing as well.
Bumps & Babies Open House Coffee Group:
Want to meet some other Pimlico Mums?? Join our Bumps & Babies Open House Coffee Group, every Tuesday morning from 10.30am-12pm.
It's really informal and a great opportunity to get out of the house for a couple of hours and meet other mums and dads in the area. Tea, coffee, biscuits and cakes are on offer.
Email if you'd like to come along and we'll send out an email with the address on Wednesday evening: firstname.lastname@example.com.
Are you a mum in the area who is in need of basic equipment/books/toys but cannot afford it?
If so, get in touch as we may be able to help! Email on firstname.lastname@example.com
For more activities and events, visit the Pimlico Parents Group page.
Whether you're expecting a baby or you already have a family with young children, NCT Westminster has a lot to offer you. We run social groups and events across the borough and offer first aid classes for babies.
Over the coming months we are aiming to increase the opportunities for social activities for parents and parents-to-be. Then we ai m to build on this to provide even more support and social activities. Please contact us if you would like to get involved.
Would you like a midwife you know and trust?
Neighbourhood Midwives can offer you just that with one to one care throughout your pregnancy, birth and the postnatal period. They are a fully insured, private midwifery organisation and you can choose from either their full care option or one of their postnatal packages.
If you decide to book with them, please quote "NCT", and they will offer you a discount of £50 and make a donation to NCT.
For more information, visit the Neighbourhood Midwives website or you can call them to arrange a free consultation on 020 8874 6624.
Westminster contacts
Main branch contact:
Tel: 0300 330 0700 | Email: email@example.com
Tel: 0300 330 0700 | Email: email@example.com
Next event
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Meade - 16 " LX200-ACF Advanced Coma-Free Telescope with UHTC
If you are into serious astronomy, whether it's as a hobby or as part of a professional research project you may be involved with, you need a telescope with certain qualities. Obviously, a Coma free telescope is preferred. But in addition, you would like something built to last, one that offers bright and crisp images, something that is easy to focus and maneuver around the night sky, and a scope with plenty of intelligence. Well, put all of those features together, add many more, and you come up with the Meade 16" LX200ACF computerized telescope.
A really smart scope should start behaving smartly right out of the box, and that's exactly what the Meade 16" LX200ACF computerized telescope does. Conventional scopes require endless hours of calibration and alignment using known objects to "fine tune" your scope. Not so for telescopes in the Mead LX200 family. Using it's intelligent AutoAlign feature, these scopes will show you that they know their way around the night sky by automatically picking up two known stars and placing them smack dab into your view finder. All you have to do now is fine tune your alignment and that's it - you're ready to get underway on your space tour.
Serious work calls for serious tools, and the 16" member of the Meade LX200 ACF family is definitely no pushover. This (literally) 320 pounds of feature-packed telescopic marvel comes with Mead's Super Giant Tripod, and ships on a pallet, and has been designed to last for a long time to come.
Any piece of equipment built like the Meade 16" LX200ACF computerized telescope needs a serious mount. And this 16" powerhouse in the Meade LX200 ACF family comes with an equally heavy duty fork mount that uses four 80mm roller bearings to deliver perfect declination (vertical) control for your scope, and two massive 150mm roller bearings to provide smooth horizontal (azimuth) movement. Using this mount you can control the swerve of your coma free telescope at a user selectable fast clip of between 1o to 6o per seconds with . 1o increments, or at a fine slew speed from .01x to 1x sidereal using 1 1/100th increments.
Avid sky watchers are like football fans watching their favorite team play a closely contended match on TV. They don't want to blink an eyelid or move a muscle for fear of missing the action. Users of the Meade 16" LX200ACF computerized telescope are the same when they are locked onto a celestial object. They can watch the action for hours. Unfortunately, minor bumps and moves could shift your regular scope off focus. But not so the Meade Coma free telescope.
Anticipating situations like this, Meade has equipped every one of its Meade LX200 scopes with technologies that specifically deliver uninterrupted long viewing pleasure to you. Pioneering features such as Zero Image Shift Microfocuser and Primary Mirror Lock technologies ensure that, once an image is locked onto by the Meade 16" LX200ACF computerized telescope, your scope never loses sight of its target even if the scope is inadvertently moved slightly. Meade's intelligent technologies help you to lock the primary mirror in place, and also helps automatically corrects the focus on an image in case it detects slight movements of the scope - which are very likely when using your Coma free telescope for extended periods of viewing.
For a telescope to really stand out, it must deliver crisp, well centered and perfectly focused images every time - there is no compromising on that requirement. The Meade 16" LX200ACF computerized telescope stands out in its use of ground-breaking optic technologies pioneered by Meade. To make your heavenly observation an experience to remember, the Meade 16" scope uses precision-driven f/10.0 ACF optics. This unique design in the Meade LX200 series uses best-in-class reflection and refraction techniques, called Catadioptrics, to deliver tightly circular formations of planetary objects from the center to the field's edge.
And that's not where Meade's optical technology stops. Each coma free telescope in the Meade LX200 series uses lenses coated with a specially created coating developed at the Meade Irvine coatings research facility. Known as Ultra-High Transmission Coatings (UHTC), this pioneering technology helps remove restrictions that you find in conventionally coated scope lenses by dramatically reducing light losses, and allowing the human eye to view heavenly objects as images that are more brighter, much more clearer and sharper. This is technology paralleling that which is used on NASA's Hubble Space Telescope, and now made available to you in the form of the Meade 16" LX200ACF computerized telescope.
To make your night sky watch a near "hands free" event, the Meade LX200 series comes with an ultra-precise original 16-channel Sony GPS receiver that is intelligent enough to input ultra-accurate coordinates for heavenly objects in the night skies, and then effortlessly align your Meade 16" LX200ACF computerized telescope to point to the specific object. Pretty neat for a 320 pound gorilla, huh?
And things get better with even more technology to help you enjoy your viewing experience. Your Meade 16" LX200ACF computerized telescope comes loaded with Meade AutoStar II, an intelligent piece of software technology that works together with the Sony GPS to effortlessly chart the night skies for you. Meade AutoStar II comprises of a flash-memory based database containing the heavenly "addresses" of over 145,000 celestial objects that are just waiting for you to explore.
But where would you start searching for one of those 100,000+ objects? Fear not, Mead AutoStar II provides you software tools to simply pick an object using Hot Key features, and stand back and enjoy the sight. Using the GPS coordinates, your Coma free telescope will automatically position itself at the selected object so that you can view it to your heart's content. And while you are enjoying the sight, Mead AutoStar II displays vital statistics about the object on its display, heightening your viewing pleasure even more.
For your continued benefit, Meade provides frequent updates and upgrades to its celestial database as well as to the software. Download the latest version free of cost, and allow Meade AutoStar II to take you on a guided tour of upcoming on in-progress celestial events, such as a comet passing through or a meteor shower.
Product Series
LX200-ACF Advanced Coma-Free Optical Tube Assemblies
|An important optional feature to optimize the performance of your Meade telescope. Image brightness in a telescope is crucially dependent on the reflectivity of the telescope's mirrors and on the transmission of its lenses. Neither of these processes, mirror-reflectivity or lens-transmission, is, however, perfect; light loss occurs in each instance where light is reflected or transmitted. Uncoated glass, for example, reflects about 4% of the light impacting it; in the case of an uncoated lens 4% of the light is lost at entrance to and at exit from the lens, for a total light loss of about 8%.
Early reflecting telescopes of the 1700's and 1800's suffered greatly from mirrors of poor reflectivity- reflection losses of 50% or more were not uncommon. Later, silvered mirrors improved reflectivity, but at high cost and with poor durability. Modern optical coatings have succeeded in reducing mirror-reflection and lens-transmission losses to acceptable levels at reasonable cost.
Meade Standard Coatings: The optical surfaces of all Meade telescopes include high-grade optical coatings fully consistent in quality with the precision of the optical surfaces themselves. These standard-equipment coatings include mirror surfaces of highly purified aluminum, vacuum-deposited at high temperature and overcoated with silicon monoxide (SiO), and correcting lenses coated on both sides for high light transmission with magnesium fluoride (MgF2). Meade standard mirror and lens coatings equal or exceed the reflectivity and transmission, respectively, of virtually any optical coatings currently offered in the commercial telescope industry.
The Meade UHTC Group: Technologies recently developed at the Meade Irvine coatings facility, however, including installation of some of the largest and most advanced vacuum coating instrumentation currently available, have permitted the vacuum-deposition of a series of exotic optical coatings precisely tuned to optimize the visual, photographic, and CCD imaging performance of Meade telescopes. These specialized, and extremely advantageous, coatings are offered here as the Meade Ultra-High Transmission Coatings (UHTC) group, a coatings group available optionally on many Meade telescope models.
In Meade catadioptric, or mirror-lens, telescopes (including the ETX-90EC, ETX-105EC and ETX-125EC; LX10, LX90, and LX200GPS Schmidt-Cassegrains; and LXD55-Series Schmidt-Newtonians) before incoming light is brought to a focus, it passes through, or is reflected by, four optical surfaces: the front surface of the correcting lens, the rear surface of the correcting lens, the primary mirror, and the secondary mirror. Each of these four surfaces results in some loss of light, with the level of loss being dependent on the chemistry of each surface's optical coatings and on the wavelength of light. (Standard aluminum mirror coatings, for example, typically have their highest reflectivity in the yellow region of the visual spectrum, at a wavelength of about 580nm.)
Mirror Coatings: Meade ETX, Schmidt-Cassegrain, and Schmidt-Newtonian telescopes equipped with the Ultra-High Transmission Coatings group include primary and secondary mirrors coated with aluminum enhanced with a complex stack of multi-layer coatings of titanium dioxide (TiO2) and silicon dioxide (SiO2). The thickness of each coating layer precisely controlled to within +/-1% of optimal thickness. The result is a dramatic increase in mirror reflectivity across the entire visible spectrum; at the important hydrogen-alpha wavelength of 656nm. - the predominant wavelength of emission nebulae - reflectivity is increased from 89% to over 97%.
Correcting Lens Coatings: Meade telescopes ordered with the UHTC group include, in addition, an exotic and tightly-controlled series of coatings on both sides of the correcting lens or correcting plate, coatings which include multiple layers of aluminum oxide (Al2O3), titanium dioxide (TiO2), and magnesium fluoride (MgF2). Per-surface light transmission of the correcting lens is thereby increased at the yellow wavelength of 580nm., for example, to 99.8%, versus a per-surface transmission of 98.7% for the standard coating.
The importance of the UHTC group becomes apparent when comparing total telescope light transmission, or throughput, caused by the multiplier, or compounding, effect of the four optical surfaces. With each optical surface contributing significantly to telescope light throughput, the effect of all four surfaces combined is indeed dramatic, as demonstrated by the graphs on the facing page, as well as by the table of the brightest nebular emission lines. At the H-alpha wavelength of 656nm., total transmission increases from 77% to 93%, an increase of 93/77 or 21% at all three nitrogen-III and sulfur-II wavelengths of 655nm. and 673nm.- prominent lines in certain galactic nuclei and in supernova remnanats such as the Crab Nebula- transmission increases by 21%; ; at the helium wavelengths of 588nm. and 469nm. - strong emission lines in hot planetary nebulae - total telescope transmission increases by 18% and 19%, respectively; at the two nitrogen II lines of 655nm. and 658nm. and at the sulfur II line of 673nm., transmission is increased by 21%. Averaged over the entire visible spectrum (450nm. to 700nm.), total light transmission to the telescope focus increases by about 20%.
Observing with the UHTC: Meade ETX, Schmidt-Cassegrain, and Schmidt-Newtonian telescopes equipped with the UHTC present dramatically enhanced detail on the full range of celestial objects - from emission and planetary nebulae such as M8, M20, and M57 to star clusters and galaxies such as M3, M13, and M101. Observations of the Moon and planets, since they are observed in reflected (white) sunlight, benefit in image brightness from the full spectrum of increased transmission. The overall effect of the UHTC is, as it relates to image brightness, to increase the telescope's effective aperture. Image brightness (i.e., the ability to see faint detail) of the Meade 10" LX200GPS is, for example, effectively increased by about one full inch of aperture.
|Emission Line
|Wavelength (nm.)
|Transmission: Standard Coatings (%)
|Transmission: UHTC Group (%)
|Hydrogen-alpha (Ha)
|Hydrogen-beta (Hb)
|Oxygen III
|Oxygen III
|Helium II
|Helium I
|Nitrogen II
|Nitrogen II
|Sulfer II
* The % increase is obtained by dividing the UHTC-transmission (column 4) by the standard coatings transmission (column 3).
Effects on CCD Imaging: While the human eye loses sensitivity to light beyond wavelengths of about 700nm., CCD imaging chips remain sensitive to about 750nm. and longer, wavelengths at which the reflectivity of an aluminum coating is near its lowpoint. Importantly, however, the UHTC's total light transmission at 750nm. is 83%, vs. 72% for standard coatings, an increase of 83/72, or 15%.
Additional Information
|Meade - 16 " LX200-ACF Advanced Coma-Free Telescope - (UHTC)
|Manufacturer Part No
|406mm (16")
|Focal Length
|Focal Ratio
|Dual fork GPS equipped mount with GOTO and Tripod
|Optical Design
|Advanced Coma-Free (ACF) with UHTC


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- Novartis appeals dispute over Neupogen copycat to Supreme Court
- A key federal appeals court decision concerning biologic drugs will unnecessarily delay biosimilar versions from entering the market, potentially costing consumers billions of dollars, Swiss pharmaceutical company Novartis AG said in a request for U.S. ...
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- In the first two cases, one involving the autoimmune-disease drug Enbrel and the other involving the cancer drug Neupogen, Sandoz tried to bypass the patent litigation provisions of the BPCIA entirely.
- The Hill Has Eyes on Biosimilars
- In the six years since the passage of the Biologics Price Competition and Innovation Act, only one biosimilar—Sandoz's Zarxio, a biosimilar of Amgen's Neupogen (filgrastim)—has been cleared by the agency. Last month, the FDA's Arthritis Advisory ...
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- Basel, Switzerland-based Novartis's move followed the decision Wednesday by the U.S.
- What Amgen's sBLA for Expanded Enbrel Use Means for the Company
- Biosimilar for Amgen's blockbuster drug Neupogen entered the US market in September last year. The biosimilar, called Zarxio, was developed by Novartis and launched at a price 15% lower than that of the branded Neupogen. The launch of a cheaper ...
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Authorities are investigating who dropped pig legs at the future site of the Al-Nur Islamic Center in Ontario. A local chapter of the Council on American-Islamic Relations has urged the government to probe the incident as a hate crime. Craig Fiegener reports for the NBC4 News at 6 p.m. on Aug. 10, 2012.
Published at 4:42 AM GMT on Aug 11, 2012 | Updated at 4:50 AM GMT on Aug 11, 2012


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SAN JOSE, Calif. - C.J. Fair scored 18 points, James Southerland added 14 and fourth-seeded Syracuse survived a second-half drought of more than 12 minutes without a field goal to beat No. 12 seed California 66-60 on Saturday night and advance to the round of 16 in the NCAA tournament.
The Orange (28-9) frustrated California with their zone defense to overcome a dismal shooting night when they made just 39 percent of their shots and missed 15 of 41 free throws.
But it proved to be enough to send Syracuse to Washington, D.C., for the East Regional where the Orange will take on the winner of Sunday's game between top-seeded Indiana and Temple on Thursday.
Richard Solomon scored 22 points for the Golden Bears (21-12), who were trying to make it to the round of 16 for the first time in 16 years and just the third time since 1960.
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