text:
Alarm Clock 188.8.131.52
Sponsored Links
Download location for Alarm Clock 220.127.116.11
Alarm Clock 22.214.171.124....read more
NOTE: You are now downloading Alarm Clock 184.108.40.206. This download is provided to you free of charge.
Select a download mirror
Alarm Clock 18.104.22.168 description
Alarm Clock 220.127.116.11. Business & Finance...read more
Alarm Clock 126.96.36.199 Screenshot
Alarm Clock 188.8.131.52 Keywords
Bookmark Alarm Clock 188.8.131.52
Alarm Clock 22.214.171.124 Copyright
WareSeeker.com do not provide cracks, serial numbers etc for Alarm Clock 18.104.22.168. Any sharing links from rapidshare.com, yousendit.com or megaupload.com are also prohibited.
Featured Software
Want to place your software product here?
Please contact us for consideration.
Contact WareSeeker.com
Related Software
A CD Player that works as an alarm clock on your PC! Works great for those with a computer near their bed. Free Download
DD-Alarm Clock is an easy to use alarm clock for your PC. Free Download
Alarm clock is a simple and useful time management software Free Download
Ossies Alarm Clock - a lightweight alarm clock/egg timer for cooking and making sure you dont miss TV programmes Free Download
create favorite image to clock Free Download
Use your Computer as an alarm clock. Free Download
Event Alarm Clock will make sure you always are on time Free Download
Alarm Clock is a free application that permits you to setup various alarms for different tasks Free Download
Latest Software
Popular Software
Favourite Software


text:
CADIZ, Spain (AP) - Unheralded Marco Crespi of Italy won his first European Tour title after carding a 3-under 69 in the final round to claim the inaugural edition of the NH Collection Open on Sunday.
Felipe Aguilar and overnight leader Matthew Nixon finished three shots back.
Crespi stumbled with back-to-back bogeys on the 12th and 13th holes, but he responded with a birdie on the 14th before making par down the stretch to take the win at La Reserva de Sotogrande Golf Club.
''I started very well today,'' said Crespi. ''I birdied the first and that gave me the confidence to go low. I just had to manage my pressure on the back nine.''
Crespi's previous best finish was fourth at the South African Open earlier this year.
Copyright 2016The Associated PressAll Rights Reserved

subset: commoncrawl


text:
You have Adblock enabled. Adblock has been known to cause issues with site functionality. If you experience any difficulties, please try disabling Adblock. Close
Add to Favorite List:
Create a new folder:
Next »
A Nun Enjoy It german ggg spritzen goo girls
Recognize a pornstar in this video?
Help make pornstars easier to find on Tube8 by telling us who is in this video.
Thanks! The pornstar names will be added to this video when enough users submit the same names.
You must enter a pornstar name that already exists in our database.
- Category: Fetish
- Views: 137,761
- Added: 4 years ago
- Description: A Nun Enjoy It german ggg spritzen goo girls
- Tags: hardcore, german, ggg, amateur
- Pornstars: Add Pornstar
- Favorite by: 70
- Featured: 4 years ago
- Report This Video
Video Comments
All Video Comments (0)


text:
realmagick.com The shrine of knowledge.
- Enviroment the big winner with Rubbish Bingo
- News. Enviroment the big winner with Rubbish Bingo. 15th Mar 2016 12:21 PM.
- "Acholi land is experiencing unprecedented destruction of its environment especially its forest cover through uncontrolled and unsustainable exploitation of its forest resources". GULU-UGANDA: The Paramount Chief of Acholi, one of the Lwo ethnic groups ...
- Listen sounds and noises of Mariana Trench noisy enviroment
- The deepest spot of ocean, Mariana Trench came as a surprise to a team of researchers who sent a titanium-encased recorder nearly seven miles below the surface to eavesdrop on the ocean floor.The ocean is a noisy place, even at its deepest spot.
- Killer Queen shows that esports can bring arcades back to life
- It's a 10-player experience that pits two teams of five against each other in an enviroment similar to the classic Joust, which tasked players with maneuvering flapping birds around the screen and killing opponents by landing on them.
- Exploration for oil, gas off NC scrapped in new federal plan
- Numerous towns, cities and counties passed resolutions opposing exploration, saying the risk to the enviroment and tourism far exceeded any economic benefits. "This is a victory for the people of the Outer Banks.
Enviroment is described in multiple online sources, as addition to our editors' articles, see section below for printable documents, Enviroment books and related discussion.
Suggested News Resources
Great care has been taken to prepare the information on this page. Elements of the content come from factual and lexical knowledge databases, realmagick.com library and third-party sources. We appreciate your suggestions and comments on further improvements of the site.
Enviroment Topics
Related searchesalice alice the summer years
the matrix revolutions hinduism
cholera susceptibility
hyperbolic paraboloid
brave new world quotes
independent democratic party
About - Contact - Advertising
© 2000 - 2016 Realmagick.com, All Rights Reserved.
© 2000 - 2016 Realmagick.com, All Rights Reserved.


text:
Final: Broncos 34, Ravens 17
The Denver Broncos took advantage of a banged-up Baltimore Ravens team to win their ninth game in a row on Sunday, totally dismantling them inside M&T Bank Stadium.
The Broncos have to be very encouraged by the fact that they went on the road against one of the better teams in the AFC and got a 17-point win.
The Broncos looked strong on both sides of the ball and created a very frustrating day for Joe Flacco and his team, who fall to 9-5, suffering their third consecutive loss.
Click forward for game grades and analysis.


text:
How 'Anchorman' would look as a classy old-school 8-bit game
No treasure chests, but plenty of Scotch.
John William "Will" Ferrell (IPA: [ˈfærəl]; born July 16, 1967) is an American comedian, actor, voice actor, and writer who first established himself as a cast member of Saturday Night Live, and has since gone on to a successful film career, starring in the comedies A Night at the Roxbury (1998), Old School, Elf (both 2003), Anchorman (2004), Kicking & Screaming (2005), Talladega Nights, Stranger than Fiction (both 2006), Blades of Glory (2007), Semi-Pro and Step Brothers (both 2008). He is considered a member of the Frat Pack, a generation of leading Hollywood comic actors to emerge in the late 1990s and the 2000s including Jack Black, Ben Stiller, Steve Carell, Vince Vaughn, and brothers Owen and Luke Wilson.
No treasure chests, but plenty of Scotch.
One actor's real life break dancing skills inspired the break dance fight scene.
Attention: Always pay Will Ferrell back for lunch.
The white pants are back, and they're tighter than ever.
Santa is down to hang, and is a Smash Mouth fan.
"The field of Republicans out there is so messed up, I figured, it makes ya miss me, doesn't it?"
Should we just...get a ruler?
'Saturday Night Live' reunion, anyone?
Let the battle for most appealing celebrity begin.
Will Ferrell would be proud.
No, this is not a belated April Fools' joke.
Kevin Hart and Will Ferrell have all the answers. Well, at least one answer.
The only thing sweeter than a Zebra Cake is Will Ferrell dressed as Little Debbie.
Don't act like you're not impressed.
Preseason baseball is finally getting more cowbell.
Alex, we'll take hilarious 'Saturday Night Live' references for $300.
Belichick would be proud.
Only Will Ferrell could improve a Beyoncé song.
He was an OK baseball player, I guess.
Here's your secret weapon, Team USA.
Finally. After months of waiting filled with mockery, hits below the belt and (we assume) furious preparation from both sides, we know who's the best drummer in the world.
A person with knowledge of the project says it's in very early development -- meaning it's got a sea turtle hatchling's chance -- but the players behind it seem serious enough
Everything is bigger in Texas, and Facebook is taking that mantra to heart, partnering with some celebrities for the upcoming SXSW music and entertainment festival in Austin, Texas, next month. Facebook announced on Wednesday it will host a Facebook ...
Two celebrity doppelgängers have waged an-all out drum war against each other -- and in the process, raised more than $300,000 for charity. The path to this grand battle of similar-looking famous people began when Will Ferrell acknowledged in his Red...
As Ron Burgundy would say, stay classy Will Ferrell. And, what the heck, informative too. While visiting Reddit to participate in an Ask Me Anything (AMA) session, the actor and comedian did just that. He paid a visit to some of Reddit's various othe...
By taking a puff from what looked like a joint on stage at the Europe Music Awards, Miley Cyrus made her twerking debacle at the VMAs sound so very three months ago. The 20-year-old lit up when she accepted an award for Best Video for "Wrecking Ball,...
The second trailer for Anchorman 2 is out -- a full minute longer than the first trailer -- and it brings further proof that it may be one of the craziest, politically incorrect comedies of the year. Will Ferrell returns as anchorman Ron Burgundy wit...
If IFC is known for anything, it's producing off-beat comedy. The new dramatic trailer for the Will Ferrell-produced miniseries The Spoils of Babylon fits that bill. The six-part dark comedy premieres Jan. 9 and stars Ferrell as Eric Jonrosh, the aut...
It has been nearly half a dozen years since Internet revelers were first graced with the hilarity of Derek Waters and Jeremy Konner's Drunk History. Since then, YouTubers and fans of Funny or Die have witnessed Michael Cera, Jack Black, Don Cheadle, ...
Ron Burgundy is still in a glass case of emotion, but now that case is made in 8-bit. In a new video, YouTuber CineFix gives Anchorman the old-school gaming treatment, which perfectly matches the cult film's retro world. SEE ALSO: The News Team Gets ...


text:
A 9x13 yellow cake with buttercream icing. I laid a piece of fondant down in the same shape as the batman so I could put the dark color icing on top of that instead of right on the cake. Then the customer could just peel it off and they didn't have to eat the dark icing.
CakeCentral.com is the world's largest cake community for cake decorating professionals and enthusiasts.
(if this is a private computer)
Yes please, register now!


text:
Rape Trial Day 2: Several Protesters Gather Outside Justice Center
STEUBENVILLE — A group of about a half-dozen protesters appeared shortly after 11:30 a.m. today outside the Jefferson County Justice Center to show their support for the alleged victim in the Steubenville rape case.
"We're here to support Jane Doe, but we're going to let Lady Justice do the speaking," said one protester, echoing comments voiced by the same group on Wednesday.
One sign delivered the lyrics of a song written to raise sexual assault awareness: "You're gonna pay for this. This is something you just can't get away with."
Another sign reminded that "the world is still watching" the case, which was evident by the national and international media camped outside of the justice center this morning.


text:
Nuffield Hospitals standardises desktops as business transformation gathers pace
Nuffield Hospitals, the UK's largest private sector hospitals group, is standardising on a single build of Windows XP following a deal to buy 1,500 desktop PCs from Dell.
This Article Covers
Data centre
Nuffield Hospitals, the UK's largest private sector hospitals group, is standardising on a single build of Windows XP following a deal to buy 1,500 desktop PCs from Dell.
Defining the datacentre needs of 21st century enterprises
A collection of our most popular articles on datacentre management, including: Cloud vs. Colocation: Why both make sense for the enterprise right now; AWS at 10: How the cloud giant shook up enterprise IT and Life on the edge: The benefits of using micro datacenters
By submitting your personal information, you agree that TechTarget and its partners may contact you regarding relevant content, products and special offers.
You also agree that your personal information may be transferred and processed in the United States, and that you have read and agree to the Terms of Use and the Privacy Policy.
The deal with Dell is the latest in a series of projects that will help Nuffield Hospitals cut its costs for most types of operations. Nuffield, which operates 41 hospitals, needs to cut its overheads substantially to be able to bid for set-tariff business from the NHS.
The new Dell OptiPlex GX520 desktops will host a single build to reduce the costs of running Nuffield's proprietary hospital information systems.
The PCs will be used when Nuffield introduces electronic procurement in the next few months.
Nuffield's general manager for IT service management Iain Roy said, "Online procurement will evolve in parallel with the expansion of Dell systems across Nuffield Hospitals' infrastructure. Dell Premier Pages offers customised web pages and price lists that give us great visibility around Dell's product portfolio."
Comment on this article: email@example.com
Enjoy the benefits of CW+ membership, learn more and join.


text:
Fancy Kicks: 10 Spectacular Sneakers to Wear Now
From Nike to Fendi.
Even if they didn't earn a Golden Peacock this Fashion Month, the street-style stars wearing fancy sneakers between shows were still winners for looking chic and comfortable at the same time. In homage, we've sought out some of the most standout sneakers for spring: shoes that you can feel comfortable pairing with cropped trousers on weekends, no heels required.
Whether you're an unrepentant white-sneaker fan or a minimalist taking on brighter styles, we've rounded up ten exceptional pairs. Scroll down to see some of our favorite options for pounding the pavement.
An everyday type of sneaker with a graphic black-and-white twist.
Pony "Topstar" Hi high-top sneakers, $65 at Madewell.
If you're praying that the white-sneaker trend will never die, check out Opening Ceremony's collaboration with Vans on this crisp shoe with a rubber sole. To keep white sneakers pristine come pollen season, use a cleaning kit like this one from Jason Markk.
Opening Ceremony x Vans "Old Skool" sneakers, $100 at Spring.
For a casual weekend look, slip on a pair of Eytys's skater-style shoes in a deep shade of green.
Eytys canvas trainers, $114 at Matches Fashion.
Nike's cult favorite Air Max shoe gets a feminine reboot this spring in a speckled lilac print.
Air Max 90 shoe, $130 at Nike.
This multicolored Adidas sneaker is a functional choice for your daily run and still pretty enough to wear off the track, too.
Adidas Ultra Boost sneakers, $26o at Saks Fifth Avenue.
Doubly cool for the two-toned wedge heel and the illusion that you're exercising more than you really are.
Melby suede espadrille trainer, $310 at Whistles.
Stella McCartney created a black sneaker for maximalists (yes, really) with a chevron heel, spiky sole, AND polka-dot mesh.
Stella McCartney faux-suede trainers, $452 at Matches Fashion.
Common Projects' "Achilles" style is a Cut staff favorite and this spring you can try it in a Pantone pink.
Woman by Common Projects "Achilles" low top, $410 at Luisaviaroma.
If you loved the thigh-high footwear in Balenciaga's show this past weekend, invest in another pair of vertically minded shoes you can wear now. These perforated high-tops come with some fun stripe-patterned decals, too.
Balenciaga monochrome perforated high-top trainers, $595 at Matches Fashion.
For a statement sneaker fit for a museum, take a peek at Fendi's Impressionistic take on street style.
Fendi printed leather sneakers, $700 at Saks Fifth Avenue.


text:
Offering exclusive yacht charter services to many beautiful destinations in many Asian countries such as Malaysia, Singapore, Hong Kong, Indonesia, Myanmar, Philippines and Thailand, Orient Charters is one of the superior yacht charter providers in Asia.
A variety style of boat charters are available including sailing holidays via crewed boat for group of friends, families and corporation to any preferable destinations. You will enjoy our different offers and attractively presented vessels that come along with wonderful service.


text:
Consolidated Edison Company History
A selection of articles related to consolidated edison company history.
Original articles from our library related to the Consolidated Edison Company History. See Table of Contents for further available material (downloadable resources) on Consolidated Edison Company History.
- Indian History Rewritten
- The Indian civilisation, considered to be the product of clash and subsequent inter mixture of the white-skinned civilised invaders and the dark savages native to India, has been rightly questioned in recent years, and the mischievously fabricated history of...
History & Anthropology >> Indic / Vedic
- The Truth Behind the History of the Necronomicon
- I don't think there are many people out there who haven't heard of the Necronomicon and the dreadful things connected with it. We've all heard the theories about how it is a Satanist tool; actual copies of the book are bound in human skin and how you risk...
Deities & Heros >> Chthulu
- History of Egyptian Medicine and Philosophy
- When medical knowledge past is considered, lack of aseptic technique, pain-killing drugs and antibiotics are an accepted part of our modern legend of these ancient healing practices. You will find that none of this is true when it comes to Ancient Egypt....
Religions >> Egyptian
- History of the Egyptian Religion, part I: Early Dynastic Period
- I - Predynastic - Early Dynastic Period. The temples were in essence the *Home of the God* and thus a place where only Pharaoh as the Son of God and those he appointed as his deputies, could meet with the god. This goes all the way back to Archaic and...
Religions >> Egyptian
- History of the Egyptian Religion, part II: The Old Kingdom
- II - The Old Kingdom ca 2686-2181 BC During the Old Kingdom the Egyptian society moved from tribal communities to a fully developed theocratic system, where the power of the king was absolute. The great pyramids of Dynasty 4 was made possible by technological...
Religions >> Egyptian
Consolidated Edison Company History is described in multiple online sources, as addition to our editors' articles, see section below for printable documents, Consolidated Edison Company History books and related discussion.
Suggested News Resources
- Southern Company: Reliable, Regular Income
- The Southern Company (NYSE:SO) is not a dividend aristocrat as Consolidated Edison (NYSE:ED) is, having a 14 year history of paying consecutively rising dividends.
- The 10 Best Dividend Stocks For Retirement
- All 10 have yield over 3%, ultra long dividend streaks, and trade at fair or better prices. Retirees want their portfolios to provide safe, consistent, reliable income that grows faster than the pace of inflation.
- The Telephone Movement: Another Point of View
- In view, however, of the interest taken in this question by many of our correspondents, we have asked one of them to put the case for the Independent Telephone companies more fully than would be possible in a letter. — THE ....
- Retirement Strategy: Getting Back To Basics
- Everyone realizes that over the course of history, the markets' trend has ALWAYS been up. That being said, I believe a more serious correction is in store, and if I am correct, the very stocks I want to add more shares of will be ...
- Sunburn for March 8 – Sine Die in sight; Budget notes galore; Latest prez
- PALM BEACH CO. SCOOPS UP NEW SPENDING via John Kennedy of the Palm Beach Post – Close to $1.
Great care has been taken to prepare the information on this page. Elements of the content come from factual and lexical knowledge databases, realmagick.com library and third-party sources. We appreciate your suggestions and comments on further improvements of the site.
Consolidated Edison Company History Topics
Related searchespg-7vr
syllogism basics
list of finance topics banking terms
active torque transfer system
singapore malay national organisation
turbocharger design details
© 2000 - 2016 Realmagick.com, All Rights Reserved.


text:
Check out what Progressive's Kaitlin Marvin, a Progressive data analyst and Millennial, had to say about three key findings in our recent survey "Inside the Minds of Millennials in IT."
1. Thinking outside the box–nearly half of respondents (42%) feel the insurance industry offers excellent opportunities in IT
As more companies amass more data (we have 14 billion miles of Snapshot data alone!), start-ups no longer corner the market on career paths in IT. The insurance industry is ripe with possibilities for you to play a direct role in maximizing the customer experience through data. During my almost three years here, I've always been encouraged to raise my hand and be on project teams that fall outside my scope of daily responsibilities. The end game? I leave the office everyday feeling really challenged and satisfied personally.
2. It's not all about the $$$–nearly three in five (58%) would rather have a more flexible work arrangement
This stat sent me back in time to when I was purchasing a new house and my schedule was nuts! I was going back and forth to meet contractors, the moving company, etc. I felt really nervous about my odd hours, but my manager told me there are times when life takes over work and vice versa. Not feeling that additional stress was so empowering! My work was being recognized and I'd earned the trust of my manager.
3. Relocation–82% of respondents reported they would move an average of 611 miles
Progressive is headquartered just outside of Cleveland, Ohio and we've been enjoying a nice run of local and national stories showcasing everything from our "foodie" scene to the boom in white collar jobs in the region. The city's orchestra and museums are some of the best in the country and when you compare cost of living with the New Yorks, Bostons and Chicagos of the world, Cleveland shines.


text:
- News tagged "intel"
Thursday, 16 Jan 2014
Intel Preparing to Refresh Haswell Lineup in Q2 2014
There are alleged plans by Intel to refresh its current Intel Core Haswell processors with a slew of new SKUs in May. In addition, the chip maker will also ship the new Z97 and H97 chipsets to board manufacturers in preparation for the next generation Broadwell processors.
Tuesday, 7 Jan 2014
Intel to Bring Wearables Into Reality with Edison
Intel is challenging developers to bring more wearables into the market through its Edison development board which is only about the size of a tiny SD card.
Microsoft Should Be Worried: Android on Windows Gets Real with Intel & AMD
Sure, we've seen dual-booting Windows/Android hybrids before. But convergence just got real at CES, as both Intel and AMD confirmed plans to help PC makers shoehorn Android onto Windows devices.
Sony Expands Vaio Fit Notebook Series with New 11-inch Vaio Fit 11A Ultra-Portable Notebook
Sony has just unveiled the 11.6-inch Vaio Fit 11A convertible ultra-portable notebook. This device is essentially an 11-inch version of the recently released Sony Vaio Fit series of convertible notebooks, with the only difference being its Intel Bay Trail-M processor.
Monday, 16 Dec 2013
Google Ponders Designing its Own Server Processors
According to reports, Google is said to be considering designing its own server processors using technology from ARM Holdings.
Friday, 13 Dec 2013
12 Days of Christmas! - The Convertible, Versatile Ultrabook
On the first day of Christmas ... we suggest a Christmas present that is convertible and versatile. The transforming Lenovo Yoga 2 Pro is a great Ultrabook and perfect for a variety of different usage scenarios. Click on through to find out more about the product and also discover how you can win these gifts this holiday season with HardwareZone!
Tuesday, 10 Dec 2013
Leaked Intel SSD Roadmap Reveals Upcoming 2TB Drives in 2014
A leaked Intel SSD roadmap has revealed the company's plans to release three new SSD series. The Fultondale and Pleasantdale series SSDs will come in 2.5-inch and PCIe add-in card formats; while the Template Star drives will be available in 2.5-inch and M.2 form factors.
Thursday, 5 Dec 2013
StarHub Giving Away Free Acer C720 Chromebook to New Subscribers
Intel has just announced that it has partnered up with StarHub to bring the Acer C720 Chromebook to Singapore. It's being offered free to customers who sign-up for StarHub's 200Mbps fibre home broadband plan, or StarHub's 5Mbps cable home broadband plan and a $5 monthly top-up.
Wednesday, 27 Nov 2013
Q3 GPU Shipment Figures Show NVIDIA's Strong Gains for Desktop Segment
GPU shipment figures for Q3 2013 have been released by Jon Peddie Research. Among the incumbent dominant players, NVIDIA had strong gains in the desktop segment. On the whole, the company's overall PC graphics shipments increased 2.3%.
Friday, 1 Nov 2013
Intel Begins Shipping LTE-Capable XMM 7160 Chip to Manufacturers
Intel has begun shipping its LTE-capable XMM 7160 chip to manufacturers. Demonstrated in June this year at Computex 2013, the XMM 7160 slim modem platform supports 15 global LTE bands, as well as 2G, 3G and voice-over-LTE services.


text:
Main Menu
Gerald Stern was born in Pittsburgh, Pennsylvania in 1925 and was educated at the University of Pittsburgh and Columbia University. He is the author of 16 books of poetry, including, most recently, Divine Nothingness (Norton, 2014) and In Beauty Bright (Norton, 2012), as well as This Time: New and Selected Poems, which won the 1998 National Book Award and a kind-of memoir of a year in 85 sections titled Stealing History, was published by Trinity University Press in the spring of 2012. Stern was awarded the 2005 Wallace Stevens Award by the Academy of American Poets, was the 2010 recipient of the Medal of Honor in Poetry by the American Academy of Arts and Letters, he was inducted into the 2012 class of the American Academy of Arts and Sciences and was the 2012 recipient of the Rebekah Johnson Bobbitt National Prize for Poetry from the Library of Congress. He was the 2014 winner of the Frost Medal. Stern has two books coming out in 2017, a poetry collection from W. W. Norton called Galaxy Love and a book of non-fiction titled Deathwatch, to be released by Trinity University Press.
Free Event
Narrated by Liam Neeson, Love Thy Nature is a cinematic journey into the beauty and intimacy of our relationship with the natural world. Neeson is the voice of Homo Sapiens - our collective humankind - who, in the past few thousand years, has come to believe that we are separate from nature. Through Sapiens' journey, the film reveals how a connection with nature ignites a sense of meaning and wonder so profound that it touches the very core of what it means to be human. Interweaving mesmerizing imagery and interview footage, Love Thy Nature is a guided tour of our relationship with nature that proposes new approaches to a sustainable future. There will be a post screening Q&A with the director Sylvie Rokab .
Hasan Elahi is an interdisciplinary artist working with issues in surveillance, privacy, migration, citizenship, technology, and the challenges of borders.
REAL BOY is the coming-of-age story of Bennett Wallace, a transgender teenager on a journey to find his voice as a musician, a friend, a son, and a man. Navigating the ups and downs of young adulthood, he works to gain the support of his mother, who has deep misgivings about her child's transition. As tension mounts at home, Bennett is taken in by his idol, Joe Stevens, the lead singer of the alt-Americana band, Coyote Grace. Exploring how our search for personal identity also involves those closest to us, REAL BOY is a nuanced look at the new American family and the people we turn to when our given families are unavailable. There will be a post screening Q&A with the director Shaleece Haas.
Liz Moore is a writer of fiction and creative nonfiction.
Her first novel, The Words of Every Song (Broadway Books, 2007), centers on a fictional record company in New York City just after the turn of the millennium. It draws partly on Liz's own experiences as a musician. It was selected for Borders' Original Voices program and was given a starred review by Kirkus.Roddy Doyle wrote of it, "This is a remarkable novel, elegant, wise, and beautifully constructed. I loved the book."
After the publication of her debut novel, Liz obtained her MFA in Fiction from Hunter College. In 2009, she was awarded the University of Pennsylvania's ArtsEdge residency and moved to Philadelphia, where she still lives.
Colm Toibin is the author of eight novels, including 'The Master' and 'Brooklyn', and two collections of stories. His play 'The Testament of Mary' was nominated for a Tony Award for best play in 2013. He is Irene and Sidney B. Silverman Professor of the Humanities at Columbia University.
It's just like book club but with albums! With new advances in technology, the way we consume music through our devices, apps and on demand streaming services like Pandora, Spotify and iTunes is making the idea of the "album" as an art form extinct. Get together with other music enthusiasts on Tuesday nights in Woods Theatre to discuss some of the greatest records of all-time! Listen to the album beforehand and then come prepared to discuss…there will be special guest moderators and panelists at each event! This discussion will feature BRUCE SPRINGSTEEN'S Nebraska. This event is free but registration is required.


text:
Diy Teeth Whitening
Once you've found out the system that greatest works in your favor, take time to usually exercise excellent dental health practices. Brush and floss your teeth all after meals, and visit your dental expert often for a knowledgeable cleaning which can check also the inmost recesses of any gums. To be honest, that's the taste of true beauty: it really is related to specifically dwell underneath.
For the following six months time I attempted a number of different teeth pastes currently available without having visible results. We even perceived getting my own teeth whiten right up by the dentist but i really could certainly not pay the outrageous pricing the dentist ended up being asking.I even looked at aesthetic dental care to whiten my tooth enamel, nevertheless the pricing were very high. After living with most of the achievable tips we considered the world wide web. Below I realized tips to get pearly white teeth by doing some investigating in to the teeth whitening procedure. Over the internet I tried to learn some affordable opportunities. I put in time exploring to be able to get glowing pearly white teeth simply by using free of cost examples of the products, and all it costs was a few bucks for shipping.
To understand more information on idol white teeth whitening reviews take a look at our personal website: idol white in stores
First, you should know of the ones that to avoid. These would be the over-the-counter gels and light/heat treatments within the dentist. Let us accomplish the gels initially. Over-the-counter ties in get many, many years to my workplace. It might take as many as fourteen days to enable one to achieve the level of lightening you would like. Even if you're certainly not in a rush, precisely do you really wait whenever you can have actually causes an extremely shorter time period? Additionally, lots of people grumble they are simply not extremely effective and had been agitated employing the information.
Second, we'll talk about the laser therapy lamp sessions that exist at a dental professional's company. Most clients state harsh discomfort and sensitiveness as soon as the method, actually to the point that they have difficulties consuming solid foods. Although lazer lamp remedies are helpful, often it are as well good. Some patients end up getting eerie-looking, unnaturally white teeth. They complain they often get teased about their teeth getting too blinding. Lastly, this procedure is pretty costly. The whole thing can run up to $600, instead of plenty of people are able to afford to invest a lot of money on the appearance.
My favorite most significant focus about 4 months earlier was actually simple awful discolored dental. I couldn't sit it because each time We see everyone they will stare inside my smile. Nobody said anything bad about any of it but strong inside I know things need to be complete. For reasons uknown, the most important opinion constantly quite important in the environment where every guide happens to be gauged by the address.
But, about three months before, we noticed a series on television about some specialist whitening products which might be utilize like a pen or serum while the prices am means less expensive than visiting the neighborhood dental expert. The lady contained in this teeth bleaching product examine revealed ways to have your tooth enamel white in color actually quite easy by just applying the whitening oxide material. These components are safe not detrimental to suit your tooth at all.


text:
BURBANK, Calif. -- In a radical move that will likely have repercussions throughout the video distribution pipeline, Buena Vista Home Entertainment here will make all of its rental products available on a shared-revenue basis through traditional distributors. The Walt Disney Co. subsidiary's goal is to increase copy depth of new releases at retail, said distribution sources. This follows copy-depth programs from Warner Home Video, also here, and Columbia TriStar Home Video, Culver City, ...
REGISTER TO VIEW THIS ARTICLE - Register for a Free Account
WhyRegisterfor FREE?
Registering for content on Supermarket News will give youINSTANTaccess to invaluable articles and media content that industry professionals rely on. You will have access to our special reports, feature articles, and industry analysis. It'sFREE, easy and quick. What are you waiting for!In addition you will also receive complimentary access to the SN salary survey data tables.
Click here to read the FAQ page if you have any questions (opens in a new window)
Attention Paid Print Subscribers: While you have already been grantedfreeaccess to SNwe ask that youregister now.We promise it will only take a few minutes! Or visit your profile and add your print magazine account number and zip code.


text:
Welcome to ScubaBoard, the world's largest scuba diving community. Registration is not required to read the forums, but we encourage you to join. Joining has its benefits and enables you to participate in the discussions.
Benefits of registering include
Joining is quick and easy. Login or Register now by clicking on the button
Log in or Sign up
Discussion in 'Fins, Masks and Snorkels' started by DaFishy, Nov 4, 2006.
i wash my snorkel after every dive so how come i have mold growing in it
What type of snorklel are we talking about ?
a moldy one!
Try washing it better, perhaps. Also, don't store it in a location that will prevent it's drying! You are washing it off, but it's probably not drying very well. Then it's stored in a dark place andthe mold starts to party. My brother left a mask in a box with some pool toys for a month or two, withit still wet. Yeah. It was moldy.
Because it's not clean and it's not dry and it has mold spores on it.
Try soaking it in water with bleach, then drying well and storing it somewhere well-ventilated and dry.
OK, I'm just asking for it I know. But if you stop diving with it you will keep it nice and dry and avoid all the mold. There are very few dives, IMHO, that require a snorkel. Snorkels are for snorkeling, we have regulators.
I know why mine has mold...because it has set in the bottom of my dive box for about a year.
Seriously though, bleach and let dry. Setting it out in the sun would be a good way to dry it.
ty every1 for the info
It is not possible to get completely rid of mold spores. You can help it by drying it out thoroughly after washing the snorkel. But you will grow things in Florida whenever you provide it a warm, moist environment. If you don't believe that, take a look at the corners of your mask too, and be prepared to be grossed out.
..........and don't examine the inside of your second stage too closely.............
hey, bleach can hurt plastics I believe. Try diluting. I think even 10% bleach would help kill the stuff. I wouldn't use more than 50%. And rinse it well when you are done, you don't want to be breathing bleach fumes next time it gets wet and the bleach becomes in solution again
Separate names with a comma.


text:
Officials with LDS Family Services made the announcement Tuesday, saying the change would provide would-be parents with more opportunities to adopt.
David McConkie, who manages the organization's services for children, tells The Salt Lake Tribune the traditional adoption agency model wasn't working for LDS Family Services. He said fewer women are putting their children up for adoption these days, likely because the stigma of being an unwed mother has subsided. Family Services has been placing 200 to 300 children per year, down from a peak of 665 in 2002. Officials tell KSL the decision is not driven by pressure to facilitate adoptions for same-sex couples.


text:
Category:Museums in Newfoundland and Labrador
From Wikipedia, the free encyclopedia
This category has the following 3 subcategories, out of 3 total.
Pages in category "Museums in Newfoundland and Labrador"
The following 11 pages are in this category, out of 11 total. This list may not reflect recent changes (learn more).


text:
goddess athena (detail view)
Pallas Athene symbolizes the creative intelligence in us all--the strategy behind the force that leads to concrete achievements. She is the goddess of foresight, planning--and wisdom.
In war, she is the Protectress of the State. Although she did not condone violence, in times of war she fought with heroism, bravery, and sensible toughness. She rules the martial arts, including fencing, aikido and t'ai chi.
She is also a significator of the healing arts. One of her many aspects was that of Hygeia, goddess of miraculous healing. Legend says that she used a vial of blood from the severed head of Medusa to revive the dead and regenerate the sick.
Goddess Athene governs therapies that integrate balance and wholeness sand that redirect the vital energy currents that flow throughout the body such as acupuncture, homeopathy, macrobiotics and cellular regeneration.
Associated symbols include the owl, spear, shield, serpent, olive tree and aegis with Gorgon's head. Associated astrology rulerships are Libra, Leo and Aquarius.
In this depiction, I have included the four higher chakras: heart, throat, ajna, and crown. As we evolve as a species, we will begin to use these higher chakras more prominently.
© 2009 Transformational Art, LLC, All Rights Reserved.


text:
Abbeville Things to Do
burt-starke mansion
the most significant historic attraction in abbeville is the armistead burt house also known as the burt-starke mansion. the armistead burt house was the site of the last council of war of cabinet members of the confederate government. on may 2 nd 1865 president jefferson davis held his final war council with secretary of war john c. breckinridge and generals braxton bragg and basil duke. davis wanted to continue the fighting, using the still plentiful forces west of the mississippi river but breckinridge and the generals disagreed. davis resigned himself to the decision of the others, effectively ending the confederacy. davis then moved south to washington georgia then was later captured by union troops in irwinville georgia on may 10 th 1865. the burt-stark mansion is listed as a national historic landmark and is open to the public by tour. for those interested in architecture and civil war history this is a must see site when in abbeville.Related to:
- Historical Travel
- Museum Visits
- Architecture
confederate civil war memorial
the abbleville county confederate civil war memorial is located on courthouse square in downtown abbeville. this memorial is an obelisk with a draped flag motif which is common in the south.Related to:
- Architecture
- Historical Travel
belmont inn
the historic belmont inn was originally named the eureka hotel and was built in 1903. the eureka catered to traveling textile industry salesman. the eureka had thirty rooms and one public bath. there was also was a barber shop and banquet rooms for trade shows. touring theather companies stayed at the eureka when performing at the opera house across the street. the eureka closed in the 1970's and reopened as the belmont inn in 1984. sadly this historic hotel has recently closed and is currently up for sale. for those interested in architecture the bellmont inn is worth a look when in downtown abbeville.Related to:
- Architecture
- Historical Travel
Abbeville Hotels
Belmont Inn
104 East Pickens St, Abbeville, SC, 29620
Satisfaction: Very Good
Good for: Business
Hearthside Manor Bed & Breakfast
1304 N. Main St., Abbeville, South Carolina, 29620, United States
Abbeville's Bed and Breakfast
602 Chestnut Street, Abbeville, South Carolina, 29620, United States
Abbeville Restaurants
Yoder's Dutch Restaurant: Treat yourself to some Mennonite Food
Yoder's is a great lunch stop when making a day trip to Abbeville or Greenwood, SC. They have an "all you can eat" dinner buffet also on Friday and Saturday nights for $9.95.
They have home cooked food that rivals any Mennonite restaurant in the area. The restaurant itself is clean and rather unadorned. There are samples of quilting and woodworking projects for sale here and there.
The buffet starts off with the dessert selection, then a garden salad bowl with the trimmings. They also have a great cole slaw and homemade applesauce. They always have chicken livers on their meat line, and hot fried chicken. The night we were there we had sweet and sour meatballs. Their vegetables always include tomatoe casserole, a bit on the sweet side, and a broccoli rice casserole. Their mashed potatoes are homemade with dark or white gravy.
They serve biscuits and homemade yeast roles. Even if you don't purchase a drink or coffee they offer a cup of coffee at the end of your meal. Nice.
We have enjoyed coming here since we moved to the Augusta area in 1978 - their food is as good today as it was 30 years ago.Related to:
- Seniors
- Road Trip
Instant Answers: Abbeville
Get an instant answer from local experts and frequent travelers
- Great room rates
- The Belmont Inn Abbeville
- Orbitz.com
- Westbrook Motel Abbeville
- Booking.com ABC Low rates
Sponsored Links


text:
- Bill & Melinda Gates Foundation – Water, Sanitation & Hygiene
- BRAC WASH Programme
- Clean Team Ghana Ltd
- Community-led Total Sanitation (CLTS)
- E-Source Sanitation News
- Emergency Sanitation Project
- Global Handwashing Day
- Hygiene Council
- India Environmental Portal – Sanitation
- India Sanitation Coalition
- India Sanitation Portal
- International Year of Sanitation 2008
- IRC – Sanitation
- Medbox – WASH and hospital hygiene
- Menstrual Hygiene Day
- News from the Poop Group – Updates from Stanford University's Poop Group
- Open defecation
- Photographs in SuSanA database
- Rice Institute – Sanitation
- SANDEC – Sanitation, Water and Solid Waste for Development
- saniblog.org
- Sanitation Marketing
- SaWi – Sanitation Window
- Selling Sanitation
- SFD – Improving Understanding of Urban Sanitation
- Sistema de Agua y Saneamiento para el Desarrollo
- Sulabh Interntional
- Sustainable Sanitation Alliance (SuSanA)
- WASH Nutrition Library
- WASHplus Project
- Wastewaterinfo.asia
- Water Supply and Sanitation Collaborative Council
- WHO – Sanitation and Wastewater
- Wikipedia – Sanitation
- World Bank – Sanitation
- World Bank – Sanitation, Hygiene and Wastewater Resource Guide
- World Toilet Association
- World Toilet Day
- World Toilet Organization
- WSUP – Sanitation
SuSanA Forum
- Re: FSM innovation challenge - iniated by The Netherlands Red Cross - by: JKMakowka October 21, 2016What does the project offer for potentially interested companies other than a chance to test it and maybe (much) later some business from the Red Cross and other NGOs? It doesn't seem like there is any direct financial support to build the prototype or something like that? I was actually working on this exact topic a few years back trying to do a PhD in […]
- Re: Time taken for faecal sludge tankers to discharge? - by: Janexi October 21, 2016Good day Sir, I work with a 4200l truck it only takes 7 to 15 minutes and it also depends with what type of effruent tht you are working on Kind regards Elvis Janexi Suppliers
- FSM innovation challenge - iniated by The Netherlands Red Cross - by: fmeulman October 21, 2016Dear everyone, Hi from a collaborative project between The Netherlands Red Cross and Open House - Amsterdam, The Netherlands. With this post I would like to create awareness of a new sanitation innovation challenge. The Netherlands Red Cross is calling on companies to present innovative solutions for faecal sludge collection and treatment, to be used during […]
- Re: Filling the gaps in integration - by: kcrampah October 21, 2016Thanks Emily.There are already institutions at the local,district, regional and global levels.Mapping out these institutions and their stakeholders can result in an array of wonderful experiences. There are bodies who lead these sectors and can be of immense help.
- Re: Filling the gaps in integration - by: kcrampah October 21, 2016I am new on this platform and like to share my thoughts on integration.My brother, who should go to who and say lets work together or this system works.If the centre does not hold together no good thing can happen and be sustained.We have done a lot in coordinating with all the various stakeholders for effective integration. Everybody expects somebody to lea […]
- Re: FSM innovation challenge - iniated by The Netherlands Red Cross - by: JKMakowka October 21, 2016
RSS: Sanitation and CLTS news
- An error has occurred; the feed is probably down. Try again later.
Add to favourites
Togo – children design handwashing stations
This entry was posted in Africa, Hygiene Promotion, Multimedia and tagged changing behaviour, handwashing, Togo, unicef. Bookmark the permalink.
2 responses to "Togo – children design handwashing stations"
I really like this design totally sustainable and created by kids its definitely the way forward
Pingback: How Do You Wash Your Hands? A Look at Simple Hand Washing Stations | HydrateLife


text:
Nationals Report 2016
The 2016 National Finals of the Russell McVeagh New Zealand Schools' Debating Championships were held from the 27th-30th of May at Victoria University of Wellington.
Twelve regional teams from Northland, Auckland, Waikato, Hawke's Bay, Central North Island, Wellington, Kahurangi-Marlborough, Canterbury and Otago-Southland took part in the competition. The regional teams comprised the best speakers from the hundreds of debaters who competed at various regional competitions held earlier in the year.
Teams competed in seven preliminary round debates on the Friday, Saturday and Sunday at Victoria University's Pipitea Campus. In the preliminary rounds the teams debated the following topics:
– This house believes that the Government should extend the student loan scheme to students who study overseas, and bond them to return to New Zealand for a period of time after they graduate
– This house would require employers to interview candidates in a way that conceals their appearance, gender, ethnicity and age.
– This house believes that Britain is better off outside the European Union
– This house would introduce a universal basic income
– This house believes that international communities should pay developing communities not to extract natural resources
– This house believes that privacy is an outdated norm
– This house would ban pharmaceutical companies from advertising their products to consumers
After the seven preliminary rounds, the top four teams were Canterbury Black, who were broke first on six wins, Wellington Gold (five wins), Wellington Black (five wins) and Auckland White (four wins). Auckland Blue were also on four wins but had slightly fewer speaker points than Auckland Blue.
On Monday morning the semi-finals were held at Wellington Girls College and Wellington High School. The motion for the semi-finals was: "This house believes that states should be allowed to pay other states to relocate and settle refugees" The top two teams qualified for the Grand Final, with Auckland White (affirming) defeating Canterbury Black in a 2-1 decision and Wellington Gold (negating) defeating Wellington Black in a unanimous decision.
The Grand Final was again hosted by Hon. Peter Dunne in the Legislative Council Chamber of Parliament. The Official Party also included Tim Clarke from Russell McVeagh. While NZSDC Alumni and current MPs Megan Woods and Jacinda Ardern were unable to attend, Opposition Leader Andrew Little was noted to have briefly stopped by during one of the competitor's speeches, perhaps to seek inspiration for the future direction of the country. The judges for the final also included recently elected MP and former NZSDC President Chris Bishop. The motion for the final was that "This house supports building state houses in areas of high socio economic wellbeing".
Wellington Gold affirmed the motion and Auckland White negated. After an excellent and very close debate, Wellington Gold were declared the winners in a 3-2 decision.
After the Grand Final the members of the 2016 Russell McVeagh New Zealand Schools Debating team were announced. The five member team is made up of Samuel Penno (Hutt International Boys' School) Peter McKenzie (Scots College), Piper Whitehead (Auckland Diocesan School for Girls), Amy Spittal (Waikato Diocesan School for Girls) and Matthew Moore (Christ's College).
The best speaker of the Championships was Amy Spittal, with Peter McKenzie being named captain of the Russell McVeagh New Zealand Schools' Debating Team.
The New Zealand Schools' Debating Council is very grateful to Russell McVeagh for its continued support of New Zealand Schools' Debating, which allows these Championships to occur. We are also grateful to Victoria University of Wellington who hosted the Championships during the weekend.
Thanks too to everyone who contributed to making the Championships such a memorable weekend – adjudicators, coaches, chairpeople, and conveners Georgina Lomax-Sawyers and Tamara Jenkin.
Here are the full list of results from Nationals 2016
Winners: Wellington Gold
Ben Stockton (Wellington College)
Ursula Crawford (Wellington East Girls' College)
Samuel Penno (Hutt International Boys' School)
Coaches: Maddy Nash & Aric Shakur
Runners-Up: Auckland White
Geneva Roy (Kings College)
Karan Kalsi (Lynfield College)
Zachary Wong (Macleans College)
Coaches: Hamish Saunders & Lucy Harrison
Highly Commended speakers:
Jeanne-Marie Bonnet (Whanganui High School)
Zachary Wong (Macleans College)
Emily Wildy (St Margaret's College)
Rosemary Dixon Cup for the Most Promising Speaker:
Piper Whitehead (Auckland Diocesan School for Girls)
Reserves to the 2016 Russell McVeagh New Zealand Schools' Debating Team:
Gabrielle Penn (Whanganui High School)
Selena Ballantyne (Columba College)
2016 Russell McVeagh New Zealand Schools' Debating Team:
Samuel Penno (Hutt International Boys' School)
Peter McKenzie (Scots College)
Piper Whitehead (Auckland Diocesan School for Girls)
Amy Spittal (Waikato Diocesan School for Girls)
Matthew Moore (Christ's College)
Andrew Stockley Cup for the Captain of the 2016 Russell McVeagh New Zealand Schools' Debating Team:
Peter McKenzie (Scots College)
Russell McVeagh Cup for the Best Speaker at the 2016 National Championships:
Amy Spittal (Waikato Diocesan School for Girls)No comments
John McGlashan wins Otago Regionals
The Russell McVeagh Otago/Southland regional debating championship was held on the weekend of the 11th and 12th of March. It was once again a stellar event where the very best of the regions secondary school debaters displayed an impressive depth of knowledge in a diverse and challenging intellectual environment.
Given the size of the Otago/Southland region, the in-round debates were spread out across both the Friday and the Saturday, with four debates on the Friday, with an additional two on the Saturday. Teams debated a wide array of topics; from whether there should be a tax on sugary drinks, to whether there should be a quota for non-white actors in award ceremonies to whether those caught for non-violent crimes should be prosecuted.
Four teams broke to the Semi Finals of the Championships. These teams were Otago Girls 1, Columba 1, John McGlashan 1 and Logan Park High School 1. Spectators were treated to two high quality debates on whether Donald Trump's candidacy was good for American politics. From the Semi FInals, Columba 1 and John McGlashan 1 emerged victorious and progressed to the Grand Final.
Before a nine judge panel and a hall filling out with a capacity for 300, the two teams were set to debate the final motion for the competition: This house regrets the fact that Governments memorialise the deaths of soldiers in past wars. In a clash that showed maturity well beyond their years, these students dealt with exceptionally respectful and well thought out cases that flowed with persuasive power. In the end however, only one could be crowned victorious, and in a 7-2 split decision, the title of champions for the 2016 Regionals competition was given to John McGlashan 1.
What follows is a list of those who were received speaker awards at the tournament:
Best Speaker
Selena Ballantyne (Columba College)
Russell McVeagh Otago Southland Nationals Team:
Selena Ballantyne (Columba College)
Joe Garry (John McGlashan)
Grant McNaughton (Logan Park High School)
Reserve to the Russell McVeagh Otago Southland Nationals Team:
Kelly Stitely (Otago Girls High School)
Most Promising Speaker
Oscar McGuire (Logan Park High School)
Highly Commended Awards
Beth Clearwater (Bayfield College)
Josh Mackay (John McGlashan)
"Gold" Squad – Year 13 development squad
- Kelly Stitely of Otago Girls
- Beth Clearwater of Bayfield College
- Josh Mackay of John McGlashan
- Louis Jennings of John McGlashan
- Oscar McGuire of Logan Park
- Casey Brown of St Hildas
- Ben Clayton of Logan Park
- Grace Sempers of Otago Girls
"Red" Squad – Year 12 and under development team 1:
- Shanti Girande of Columba
- Anna Roberts of Kavanagh
- Lydia Christensen of Columba
- Isabelle Robertson of St Hildas
- Rebecca MacDonnell of Columba
- James Williamson of Otago Boys
"Green" Squad – Year 12 and under development team 2:
- Lydia Joseph of Columba
- Sabrina Swerldorff of Otago Girls
- Tom Acklin of Bayfield College
- Anna Dawes of Otago Girls
- Cam Olsen of Bayfield College
- Beth Fitchett of St Hildas
No comments
Kerikeri dominate Northland Regionals again
The Northland Regional Debating Competition was held on the 28 February at Kerikeri High School. 9 teams travelled from around Northland to attend.
This year's tournament was very high quality, with three preliminary rounds on topics such as imposing a sugar tax, and the media's focus on the private lives of politicians. Following these three debates, Huanui 1 and Kerikeri High School 1 progressed to the Grand Final.
The Grand Final topic was "that major film and television awards should have racial quotas for nominating non-white actors." Congratulations to Kerikeri 1 for successfully affirming the motion and taking out the competition.
A big thank you to the judges who made the long trek to Kerikeri and teachers who gave up their day for the tournament, and to Kerikeri High School who generously provided the venue.
Winners: Kerikeri High School
Runners-Up: Huanui College
Best Speaker:
Aimee Leaming (Kerikeri High School)
Russell McVeagh Northland Regional Schools' Debating Team:
Aimee Leaming (Kerikeri High School)
Ella Short (Huanui College)
Laila Abada (Kerikeri College)
Reserve to the Russell McVeagh Northland Regional Schools' Debating Team:
Ella Rankin (Huanui College)
Hamilton Boys win Waikato Regionals in 2016
The Waikato Regional Debating Competition was held on the 5th of March at Waikato University. 18 teams travelled from around the Waikato and Bay of Plenty to attend.
This year's tournament was very high quality, with three preliminary rounds on topics such as racial quotas in film awards and the banning of climate change denial. Following these three debates, four schools progressed to the semi-finals: Waikato Dio, Hamilton Boys High School, Tauranga Boys College, and Hillcrest High School.
Waikato Dio and Hamilton Boys High School were the two teams in an entertaining and closely fought final on the motion that "this house regrets the media's focus on the personal lives of politicians." Congratulations to Hamilton Boys for winning the debate and the competition overall.
A big thank you to all the judges and teachers who gave up their day for the tournament, and to Waikato University who generously provided lunch and a venue.
Winners: Hamilton Boys High School
Jacob Cheatley
Tim Ng
Alex LeComte
Runners-Up: Waikato Diocesan School for Girls
Emma Harris
Emma Fountaine
Amy Spittal
Promising Speakers:
Jonathan Tomlinson (Mt Maunganui)
Jonah Franke Bowell (Hillcrest High School)
Highly Commended:
Zara Olifent (Hillcrest High School),
Aneesha Dahya (John Paul College),
Emma Fountaine (Waikato Diocesan)
Reserves to the Waikato Regional Schools' Debating Team:
Timothy Ng (Hamilton Boys High School)
Hugo Thompson (Tauranga Boys High School)
Waikato Regional Schools' Debating Team:
Amy Spittal (Waikato Diocesan),
Alex Le Comte (Hamilton Boys High School)
Andrew Devitt (Tauranga Boys High School)
Best Speaker:
Amy Spittal (Waikato Diocesan)
Christ's College wins Canterbury Regionals 2016
The Russell McVeagh Canterbury Regional Debating Championships were held on the 12th and 13th of March this year at the University of Canterbury and again showed the depth and breadth of debating talent in the region.
Fifteen teams from ten schools from around the Canterbury region competed in four preliminary rounds held on Saturday and Sunday morning and debated the merits of unpaid internships, home schooling, the media's focus on the personal lives of politicians and giving development aid to non-democratic governments.
At the end of the fourth round the four top teams advanced to the semi-finals which were held on Sunday afternoon. Christ's College 1 advanced undefeated on four wins while St Andrew's College 1, St Margaret's College 1 and Papanui High School 1 advanced on three wins.
The motion debated by teams in the semi-finals was "this house believes that the state should be liable for the injuries and deaths of homeless people." Christ's College 1 beat Papanui High School 1 in a split decision while St Andrew's College 1 defeated St Margaret's College 1.
The final was debated on whether or not Donald Trump was good for United States politics and in a close debate Christ's College 1 (Matthew Moore, Hamish Thomas and Angus Dysart-Paul) prevailed over runners-up St Andrew's College 1 (April Oakley, Harrison Smith and Isabella Garbutt).
Selectors and coaches for the Regional teams were impressed by the quality of debates and selected two teams to compete at the New Zealand Debating Championships in May. The speakers selected were:
Canterbury Black
Matthew Moore (Christ's College)
Angus Dysart-Paul (Christ's College)
Emily Wildy (St Margaret's College)
Canterbury Red
Harrison Smith (St Andrew's College)
Madeline Tilley (St Margaret's College)
Chloe Goodsir (Papanui High School)
Isabella Garbutt (St Andrew's College)
Sophie Harrison (Papanui High School)
A number of speaker prizes were awarded to individual debaters who excelled throughout the tournament.:
Best Speaker
Matthew Moore (Christ's College)
Highly Commended
Zachary Withers (Burnside High School)
April Oakley (St Andrew's College)
Amy Huang (Rangi Ruru Girls' School)
The trophy for the Most Promising Team was also awarded which is awarded annually to a team that does exceptionally well that is comprised of debaters who are not in Year 13. This year the award was awarded to St Margaret's College 3 which consisted of Aisha O'Malley, Millie O'Brien and Ruby Anderson who are all in Year 11.No comments
Wanganui win CNI Regionals in 2016
The Central North Island Regional Tournament was held on the 12th and 13th of March at Saint Peter's College in Palmerston North. 18 teams travelled from around the region to attend the tournament, the most the region has seen in a number of years.
The calibre of the tournament was incredibly high, with five preliminary rounds on the Saturday and Sunday to determine who would break for the semi-finals. Debate motions ranged from the popularity of reality television in New Zealand to the memorialisation of soldiers.
The four teams who broke were Wanganui High School A, Palmerston North Girls High School 2, Collegiate, and Wanganui High School B. From these semi-finals, Wanganui High School B and Collegiate progressed through to the finals.
The motion for the final round was "that major film and television awards should have racial quotas for nominating non-white actors" with Wanganui High School affirming, and Collegiate negating. After a high quality debate, Wanganui High School were announced as the unanimous winners of the Central North Island regional competition.
An additional thank you to the adjudicators who travelled for the tournament.
Winners: Wanganui High School B
Jeanne-Marie Bonnet
Connor Cresswell
Jack Southee
Runners-Up: Collegiate
Grayson Nepia
James Ensor
Jonathon de Jongh
Best Speaker
Gabrielle Penn (Wanganui High School)
Most Promising Speaker
Denzel Chung (Palmerston North Boys' High School)
Promising Speakers
Grayson Nepia (Collegiate)
Aidan Berkhan (Palmerston North Boys' High School)
Highly Commended
Catherine Reynolds (Saint Peter's College)
Kaia Costanza-van Den Belt (Palmerston North Girls' High School)
Central North Island Squad
Gabrielle Penn (Wanganui High School)
Jackie Hazelhurst (Wanganui High School)
Denzel Chung (Palmerston North Boys' High School)
Milo Costanza-van Den Belt (Palmerston North Boys' High School)
Alister Hughes (Wanganui High School)
Jeanne-Marie Bonnet (Wanganui High School)
Applications for Coaching and Judging WSDC in 2016
The New Zealand Schools' Debating Council will be sending a team to WSDC in Stuttgart, Germany between 19 – 29 July 2016. The Council is looking for suitably qualified coaches and adjudicators from New Zealand to attend the tournament for its duration.
Council is opening applications for the NZ team coaches for up to 2 coaches. Council will be subsidising each coach so that coaches based in New Zealand will only have to contribute $1000. The subsidy will include the registration for each adjudicator (which covers accommodation, transportation, attendance at Worlds social events and most meals).
- Attendance at Worlds from 18th – 29th July.
- Training your team: Coaches will be required to train the team prior to the announcement. Council has a coaching budget available to compensate you for your costs in travelling for coaching.
- Whether you are interested in coaching
- Whether you are applying as an individual, have a preference as to who you coach with, or are applying as a combination.
- Anything else you would like us to know in order to inform the decision (e.g. coaching, debating, adjudicating, familiarity with the schools style).
To support the attendance of NZ adjudicators at the tournament the Council is opening applications for up to 3 subsidized adjudicators.
The Council will be subsidizing each adjudicator so that adjudicators based in New Zealand will only have to contribute $1500. The subsidy will include the registration for each adjudicator (which covers accommodation, transportation, attendance at Worlds social events and most meals) and flights to Germany.
Adjudicators need to have experience at judging debates in the WSDC-style (the same style used at our national and regional competitions), command respect as an adjudicator, and be able to deliver adjudications in front of large school audiences. Adjudicators will also be expected to play a role supporting the NZ contingent at Worlds. Any applicants who, in the view of the Council, do not meet those requirements may not be selected as a representative NZ adjudicator eligible for the subsidy.
Please submit your application to the Vice President of the Council ([email protected]) by 10pm on 15 April 2016. Please also provide debating/adjudicating CV and referees.
The Council will advise successful applicants for both the coaching and adjudicating positions of a result by 22 April 2016.No comments
Auckland Diocesan School for Girls enjoy victory at Auckland Regionals 2016
Auckland's most enthusiastic and proficient debaters gathered for the Russell McVeagh Auckland Regional Debating Championships on the 19th and 20th of March. Some 28 teams from a diverse range of schools competed in the tournament, hosted by Auckland Grammar School.
Over four preliminary rounds on Saturday, students debated issues including banning unpaid internships, creating term limits for MPs in New Zealand, ending the provision development aid to undemocratic governments, and the topical question of whether awards shows such as the Oscars should have quotas for nominating non-white actors. The pool of adjudicators were incredible impressed with how well all teams grappled with such contentious, pertinent, and important issues.
This quality of debate was reflected in the quality of the teams breaking to the knockout rounds, held on Sunday. Like the previous year, one team came out of Saturday unbeaten; however, this year it was Lynfield College 1 that took the mantle. They were followed by eight teams on three wins, one of whom (Sacred Heart 2) had to miss out on the quarter finals on the basis of speaker points. The diversity of strength at this year's tournament was also exemplified by the fact that all eight quarter-final places were filled by different school; no single school managed to break more than one team into the knockout rounds.
In the quarter-finals, Saint Kentigern College 2, Howick College 1, King's College 2, and Macleans College 1 all bowed out after tight losses on the in a debate about providing funding directly to iwi for the provision of state services such as education and welfare. On a motion about doctors, rather than parents, making important medical decisions for children, Westlake Boys High School 2 narrowly lost their semi-final in a 2-1 split decision to Lynfield College 1, while Saint Cuthbert's College 1 were edged out by Auckland Diocesan 1.
The final was held in front of an enthusiastic audience on the motion 'This house believes that party leaders should be elected by their members rather than caucus', Diocesan 1 came out the victors in a unanimous decision, negating the motion.
The quality of the tournament was also reflected in the need to invite a number of speakers back on the Sunday for a series of 'selection debates', culminating in the selection of six outstanding speakers (listed below) for the two Auckland Regional Debating Teams, who will compete at the Russell McVeagh New Zealand Schools Debating Championships in May.
Best Speaker
Karan Kalsi (Lynfield College)
Auckland Blue
Ford Gooch (Westlake Boys High School)
Umbar Sandhu (Howick College)
Piper Whitehead (Auckland Diocesan)
Auckland White
Geneva Roy (King's College)
Karan Kalsi (Lynfield College)
Zachary Wong (Macleans College)
Reserves to the Regional teams
Sam Baker (Westlake Boys High School)
Tiffany Goh (Saint Cuthbert's College)
Highly Commended Speakers
Hogan Wang (Auckland Grammar School)
Tate McGregor (Saint Kentigern College)
Jia Dua (Lynfield College)
Most Promising Speaker
Harry Ashley (Lynfield College)
Wellington College win Wellington Regionals
The Wellington Regional Debating Championship was held over the 19th and 20th of March at Wellington Girls' College. This year we had entries from 16 extremely high quality teams from around the wider Wellington Region.
Five preliminary rounds were held across Saturday and Sunday. Teams debated topics ranging from whether a tax should be implemented on sugary food and drink, to whether quotas should be introduced for non-white actors at the Oscars.
The four teams that broke to the semi-finals were Wellington College, Scots College, Queen Margaret College and Wellington East Girls' College. Hutt International Boys' School were very close to breaking but missed out on speaker points. The teams debated whether iwi should be given funding to provide social services, such as education and welfare, directly. Wellington College and Scots College progressed through to the final from two very good debates.
The final was held in front of a large, enthusiastic crowd. The motion was "That the US should intervene militarily in Syria". Scots College affirmed and Wellington College negated. After an excellent debate Wellington College were unanimously crowned the Wellington champions for 2016.
From the weekend two teams were selected to represent Wellington at Nationals. Special thanks to all the adjudicators who gave up their time to judge over the weekend. A big thank you also to Wellington Girls' College for hosting yet again.
The results and speaker awards for the tournament are listed below:
Winners: Wellington College
Ben Stockton
Nick Webster
Tim Rutherford
Alex Sinclair
Runners Up: Scots College
Lachlan Parlane
Andrew Tang
Manraj Rahi
Peter McKenzie
Best Speaker
Peter McKenzie (Scots College)
Wellington Black
Peter McKenzie (Scots College)
Michael O'Brien (Wellington College)
Brooke Kinajil-Moran (Wellington Girls' College)
Wellington Gold
Ben Stockton (Wellington College)
Sam Penno (HIBS)
Ursula Crawford (Wellington East Girls' College)
Reserve to the Wellington teams:
Anya Bukholt-Payne (Wellington High School)
Highly Commended Speakers
Sam Porta (HIBS)
Catherina Lee (St Catherine's College)
Finn Connell (HVHS),
Promising Speaker:
Alvien Benitez (HIBS)
Applications Open for the Chief Adjudicator, Adjudicators and Motions Committee for the Nationals Finals
The New Zealand Schools Debating Council has now opened applications for the following positions for the 2016 National Finals of the Russell McVeagh New Zealand Schools' Debating Championships:
- Chief Adjudicator of the National Finals (1)
- Adjudicators of the National Finals (no set number)
- Motions Committee for the National Finals (no set number)
Below you will find role descriptions for each of the above three positions. To apply, please send a short debating and/or adjudication CV to the Vice President of the New Zealand Schools' Debating Council ([email protected]
Chief Adjudicator
The Chief Adjudicator heads the Motions Committee for Nationals (explained below). They also advise the Council on the appointment of adjudicators for Nationals. During the weekend the Chief Adjudicator is responsible for the allocation of adjudicators to debates (preliminary rounds, semi-finals and Grand Final) and the running of the draw. The Chief Adjudicator is also head of the panel which selects the Russell McVeagh New Zealand Schools' Debating Team.
The Chief Adjudicator obviously must be available to be at Nationals in Wellington from 27 – 30th May. The Council will meet the flight and accommodation costs of the Chief Adjudicator should they hail from outside Wellington.
We are also looking for adjudicators for Nationals.
If you:
- are experienced at judging schools' debates;
- can deliver articulate oral adjudications;
- command respect as an adjudicator; and
- are available to be in Wellington from Friday 27 – Monday 30 May;
Then we'd love to hear from you.
Judges from outside of Wellington will have their flight and accommodation costs covered by the Council.
Motions Committee
The Motions Committee consists of the Chief Adjudicator and up to 4 individuals. The Committee decides all the motions used at Nationals.
To apply for any or all of the above positions, please send a short debating and/or adjudication CV to the Vice President of the New Zealand Schools' Debating Council ([email protected]


text:
Karl Lagerfeld Was Really on a Roll Last Night
<span>"It's a joke. It's an inspiration. In a way, it's me."</span>
Karl Lagerfeld celebrated his new Olympics-themed collection at Selfridges in London last night, where he said and did a whole bunch of hilarious things amid an entourage of strapping, handsome bodyguards. Naturally, he was subjected to a barrage of questions on subjects ranging from Kate Middleton to the Olympics to Choupette, and so we've done you the favor of consolidating all his quotes in one place.
I think inspiration is a muscle. You must use this muscle all the time. The more you do, the more ideas you have. I don't believe in waiting for inspiration on beaches. I believe in the job, on the spot.
On seeing people dress like him:
It's difficult for me to say because it's me. It's a strange feeling that I'd rather not analyse but I am very pleased.
She has a divine silhouette so she can wear anything. She has a sense of what's right for the moment because you can not dress like a mad woman in those situations.
I never expected to like a cat because I've had dogs in the past but Choupette is fragile. She goes to the doctors every 10 days to check her weight and see if everything is okay. She even has a personal maid ... She's very clever. Generally cats don't speak but when she wants something, she just sits in front of it. It's unbelievable ... Her fur is so beautiful. She's like a huge powderpuff with blue eyes. People are stunned by her ... Soon people will talk more about Choupette than about me!
On working with Victoria Beckham:
I'm a great admirer of hers. She's a great example to tons of women; to have her four children, an incredible look and she's clever. I know her from her days as a Spice Girl. Her designs are not bad at all, they're good. She's totally unpretentious. She brought one of her sons with her [on the shoot] and he was the sweetest boy, because often children of celebrities aren't pleasant. This boy was perfection ... He's handsome, cute, polite. I know children of celebrities and I can tell you, you can keep them at home.
On his Olympics-themed collection:
It's a joke. It's an inspiration. In a way, it's me. So I have to be careful not to get too much into it, because I don't expect everybody to dress like me. That's why I like this collection with the t-shirt to make a joke out of me, because I think I'm a cartoon.
He is a cartoon! A discus-clutching character, to be exact.


text:
Molecular Imaging of Cardiac Cell Transplantation in Living Animals Using Optical Bioluminescence and Positron Emission Tomography
Jump to
Background— The current method of analyzing myocardial cell transplantation relies on postmortem histology. We sought to demonstrate the feasibility of monitoring transplanted cell survival in living animals using molecular imaging techniques.
Methods and Results— For optical bioluminescence charged-coupled device imaging, rats (n=20) underwent intramyocardial injection of embryonic rat H9c2 cardiomyoblasts (3×106 to 5×105) expressing firefly luciferase (Fluc) reporter gene. Cardiac bioluminescence signals were present for more than 2 weeks with 3×106 cells: day 1 (627 000±15%), day 2 (346 100±21%), day 4 (112 800±20%), day 8 (78 860±24%), day 12 (67 780±12%), and day 16 (62 200±5% p · s−1 · cm2−1 · sr−1). For micro–positron emission tomography imaging, rats (n=20) received cardiomyoblasts (3×106) expressing mutant herpes simplex type 1 thymidine kinase (HSV1-sr39tk) reporter gene. Detailed tomography of transplanted cells is shown by 9-(4-[18F]-fluoro-3hydroxymethylbutyl)guanine ([18F]-FHBG) reporter probe and nitrogen-13 ammonia ([13N]-NH3) perfusion images. Within the transplanted region, there was a 4.48±0.71-fold increase of in vivo [18F]-FHBG activity and a 4.01±0.51-fold increase of ex vivo gamma counting compared with control animals. Finally, the in vivo images of cell survival were confirmed by ex vivo autoradiography, histology, immunohistochemistry, and reporter protein assays.
Conclusions— The location(s), magnitude, and survival duration of embryonic cardiomyoblasts were monitored noninvasively. With further development, molecular imaging studies should add critical insights into cardiac cell transplantation biology.
Received April 23, 2003; de novo received June 24, 2003; revision received July 24, 2003; accepted July 26, 2003.
Ischemic heart disease accounts for the majority of all cardiovascular deaths. Despite advances in medical therapies, some patients develop refractory symptoms. Over the past decade, growing evidence from animal studies suggests that cell therapy can improve cardiac function.1 These encouraging data led to recent clinical reports showing beneficial improvement from intramyocardial injections of skeletal myoblasts and transendocardial injections of bone marrow stem cells.2 However, the assessment of cell fate and survival in vivo remains elusive. In animal studies, cells labeled with a conventional reporter gene (eg, green fluorescent protein) or a thymidine analogue (eg, bromodeoxyuridine [BrDU]) require postmortem histology at given time points. In clinical studies, the interpretation of therapeutic efficacy often relies on indirect echocardiographic measurements and subjective symptom reports by patients. Thus, there is a strong impetus for developing novel imaging approaches that would allow noninvasive assessment of myocardial response to cell therapy.3,4
Virus Construction and Amplification
Replication-defective recombinant adenovirus carrying cytomegalovirus promoter driving 2 reporter genes was constructed and amplified as described (Ad-CMV-Fluc and Ad-CMV-HSV1-sr39tk).5,6 Henceforth, the Fluc enzyme is referred to as "FL" and the HSV1-sr39tk enzyme as "HSV1-sr39TK."
Cell Culture and In Vitro Assays for FL and HSV1-sr39TK
Embryonic rat H9c2 cardiomyoblasts (American Type Culture Collection) were cultured as described.7 Cells were transfected (multiplicity of infection 250) with Ad-CMV-Fluc or Ad-CMV-HSV1-sr39tk overnight. The virus supernatant was removed and the cells washed 3 times with phosphate-buffered saline before adding trypsin for cell collection. Afterward, reporter enzyme activities were confirmed before cell transplantation. The Luciferase Assay Reagent (Promega) was used to determine FL level as described.5 Cardiomyoblasts transfected with Ad-CMV-HSV1-sr39tk serve as a negative control. The penciclovir phosphorylation assay was used to determine HSV1-sr39TK level as described.6 Cardiomyoblasts transfected with Ad-CMV-Fluc served as a negative control. All experiments were performed in triplicates.
Intramyocardial Transplantation of Embryonic H9c2 Cardiomyoblasts
Female nude athymic rats (weighing 200 to 300 g; Charles River Laboratories, Wilmington, Mass) were studied under protocols approved by the UCLA Animal Research Committee. Harvested cardiomyoblasts expressing either FL or HSV1-sr39TK reporter proteins were kept on ice for <15 minutes for optimal viability before injection (n=50 rats). The aseptic lateral thoracotomy and postoperative care procedure have been described previously.6 The surgical mortality rate was ≈15%.
Optical Bioluminescence and Micro–Positron Emission Tomography Imaging of Cardiomyoblast Transplantation
Optical bioluminescence imaging was performed using the charged-coupled device camera (Xenogen).5 After intraperitoneal injection of reporter substrate d-Luciferin (375 mg/kg body weight), rats were imaged for 30 minutes using 30×1-minute acquisition scans. The same rats were scanned every 1 to 4 days for more than 2 weeks. Bioluminescence was quantified in units of photons · second−1 · centimeter2−1 · steridian−1.4 Cardiac positron emission tomography (PET) imaging was acquired using a prototype microPET scanner.6 Perfusion tracer [13N]-NH3 (2.35±0.35 mCi) was injected via the tail vein and rats imaged for 20 minutes. The rats were then injected with [18F]-FHBG (2.55±0.51 mCi), and images from 60 to 80 minutes after injection were reconstructed by filtered back-projection algorithm.6
Gamma Well Counting and Digital Autoradiography
After imaging, explanted hearts were counted for [18F] radioactivity in a gamma well counter (Cobra II Auto-Gamma).6 Afterward, hearts were frozen in liquid nitrogen and embedded in 5% carboxymethylcellulose. The cryosections were exposed directly onto digital plates and scanned on a Fuji Bas 5000 digital phosphorimager as described.4
Histology and Immunohistochemistry
Explanted hearts underwent formalin fixation, paraffin sectioning, hematoxylin and eosin staining, and immunohistochemistry. Sections were incubated with primary antibody for troponin T, BrDU (10 mmol/L; overnight incubation in cell culture), and α-smooth muscle actin, followed by secondary antibody (rat adsorbed anti-mouse, 1:1000 dilution). Visualization was accomplished using an avidin-biotin-peroxidase complex as described by manufacturer (Vector Labs).
Data Analysis
Data are given as mean±SD. For statistical analysis, the 2-tailed Student's t test was used. Differences were considered significant at P<0.05.
Optical Bioluminescence Imaging of Cell Transplantation
To understand the physiological pattern of cell survival, transplanted cardiomyoblasts (3×106) expressing Fluc reporter gene were imaged repetitively over a 2-week period in the same animals (n=5). Control rats transplanted with cardiomyoblasts expressing HSV1-sr39tk show background signal only (n=5) (Figure 1A). To assess the detection threshold, different numbers of cardiomyoblasts (2×106, 1×106, and 5×105; n=5 each) were also injected. Cardiac signals were clearly present even in the 5×105 group: day 1 (112 500±17%), day 2 (59 670±15%), day 4 (21 420±10%), day 8 (18 660±18%), day 12 (9123±4%), and day 16 (7070±6%) versus day 2 control rat (1306±13% p · s−1 · cm2−1 · sr−1) (P<0.01). Drastic reduction of signal intensity was seen within the first 1 to 4 days for all groups of animals, especially those that received larger doses of cells.
MicroPET Imaging of Cell Transplantation
To assess the feasibility of monitoring cell transplant using the radionuclide approach, transplanted cardiomyoblasts (3×106) expressing HSV1-sr39tk reporter gene were imaged longitudinally in the same animals (n=20). Control rats transplanted with cardiomyoblasts expressing Fluc show background signal only (n=5) (Figure 1B). MicroPET imaging shows detailed tomographic location of transplanted cells when the [18F]-FHBG reporter activity image is overlaid on the [13N]-NH3 perfusion image (Figure 1C). At day 2, regions of interest drawn over [18F]-FHBG activity show significant differences at the transplanted region (0.056±0.014) compared with its contralateral septum (0.019±0.002) and control rats (0.013±0.001 % injected dose/g) (P<0.0001).
Validation of In Vivo Imaging Results With Traditional Ex Vivo Assays
The presence of transplanted cardiomyoblasts on in vivo images was confirmed by several independent methods. First, the ex vivo gamma well counting showed a 4.01±0.51-fold increase of trapped [18F] radioactivity in study rats (0.038±0.017) compared with control rats (0.009±0.005% ID/g) (P<0.05), consistent with the in vivo region of interest analysis. Second, the high-resolution ex vivo autoradiography confirmed the anatomic location of transplanted cells on microPET image (Figure 1D). Third, the in vitro HSV1-sr39TK reporter protein assay in the transplanted region (0.420±0.111) was significantly higher compared with its contralateral septum (0.057±0.011) and control rats (0.020±0.010% conversion/μg per min) (P<0.05). Finally, the microPET location of transplanted cardiomyoblasts correlated with hematoxylin and eosin stain (Figure 2A). Numerous cells undergoing apoptosis can be seen at higher power of magnification (Figure 2B). Immunohistochemical studies of these embryonic cardiomyoblasts stained negative for troponin T (marker for adult heart muscle), positive for α-smooth muscle actin (marker for embryonic heart muscle), and positive for BrDU (marker for DNA synthesis) (Figures 2C through 2F).
This is the first study to demonstrate the feasibility of monitoring transplanted cells in the myocardium of living animals using reporter gene imaging technology. The location(s), magnitude, and survival duration under real-time physiological influences were monitored over 2 weeks. Drastic reductions of signal intensity within the first 1 to 4 days after transplantation were noted, likely because of acute donor cell death from inflammation, ischemia, and apoptosis as shown on histology.1,3 These survival kinetic curves are consistent with other studies using serial terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) apoptosis assay, TaqMan polymerase chain reaction, and immunohistological samples obtained from a large number of animals euthanized at different time points.8,9 Under the given conditions, optical imaging has a very high detection threshold, whereas microPET imaging provides detailed anatomic locations of transplanted cells. Most importantly, in vivo imaging results were confirmed by ex vivo gamma counting and enzyme assays and correlated to autoradiography, histology, and immunohistochemistry.
Several imaging strategies are being actively investigated. Kraitchman et al10 recently demonstrated the feasibility of monitoring magnetically labeled mesenchymal stem cells using MRI. In the present study, our group has focused on labeling transplanted cardiac cells with different reporter genes for multimodality imaging purposes. Several other reporter genes, such as the dopamine type 2 receptor, somatostatin receptor, and synthetic renilla luciferase, have already shown promising results for labeling tumor cells in animal studies using PET, single photon-emission computed tomography, and optical bioluminescence imaging.4 Thus, the wide assortment of reporter genes opens up the possibility for further cardiac studies whereby different stem cells can be labeled with different reporter genes and their engraftment, survival, and differentiation analyzed individually.1,3,4
In our proof-of-principle study, rats underwent intramyocardial injection of embryonic cardiomyoblasts. Issues such as the optimal cell type, cell number, and delivery route will need to be addressed.1,3 Likewise, detection sensitivity and signal quantification for each imaging modality will have to be elucidated. With transient adenoviral transfection, the in vitro reporter protein activity declines after 5 days, which may have further contributed to signal reduction.7 Ongoing studies will involve stable transfection of stem cells.4 This approach will need to be carefully investigated before application in human disease. Encouragingly, use of genetically modified stem cells secreting vascular endothelial growth factor has been shown to add additional cardioprotective benefits.11 This ex vivo manipulation allows one to link any therapeutic gene of interest to a reporter gene.4 Thus, reporter gene imaging may potentially be used to monitor combined cell transplantation and gene therapy.
In summary, in vivo imaging of cardiac cell transplantation provides obvious advantages over traditional techniques. These noninvasive approaches will allow investigators to rapidly evaluate many important parameters, determine the efficacy of repeated transplantations, and screen for novel drugs designed to reduce acute donor cell death or apoptosis. With further refinement, molecular imaging studies will likely contribute to the goal of clinical transplantation protocols that are reproducible, beneficial, and quantifiable.
This work was supported by funding from the National Institutes of Health (RO1 CA82214-01 to Dr Gambhir), Small Animal Imaging Resource Program (R24 CA92865 to Dr Gambhir), Department of Energy (DE-FG02-03ER63687 to Dr Gambhir), American Heart Association (0120090Y to Dr Wu), and GlaxoSmithKline (cardiovascular grant to Dr Wu).
- ↵
Orlic D, Hill JM, Arai AE. Stem cells for myocardial regeneration. Circ Res. 2002; 91: 1092–1102.
- ↵
Perin EC, Dohmann HF, Borojevic R, et al. Transendocardial, autologous bone marrow cell transplantation for severe, chronic ischemic heart failure. Circulation. 2003; 107: 2294–2302.
- ↵
- ↵
Massoud TF, Gambhir SS. Molecular imaging in living subjects: seeing fundamental biological processes in a new light. Genes Dev. 2003; 17: 545–580.
- ↵
Wu JC, Inubushi M, Sundaresan G, et al. Optical imaging of cardiac reporter gene expression in living rats. Circulation. 2002; 105: 1631–1634.
- ↵
Wu JC, Inubushi M, Sundaresan G, et al. Positron emission tomography imaging of cardiac reporter gene expression in living rats. Circulation. 2002; 106: 180–183.
- ↵
Bengel FM, Anton M, Avril N, et al. Uptake of radiolabeled 2′-fluoro-2′-deoxy-5-iodo-1-beta-D-arabinofuranosyluracil in cardiac cells after adenoviral transfer of the herpesvirus thymidine kinase gene: the cellular basis for cardiac gene imaging. Circulation. 2000; 102: 948–950.
- ↵
- ↵
Reinecke H, Zhang M, Bartosek T, et al. Survival, integration, and differentiation of cardiomyocyte grafts: a study in normal and injured rat hearts. Circulation. 1999; 100: 193–202.
- ↵
Kraitchman DL, Heldman AW, Atalar E, et al. In vivo magnetic resonance imaging of mesenchymal stem cells in myocardial infarction. Circulation. 2003; 107: 2290–2293.
- ↵
This Issue
Jump to
Article Tools
- Molecular Imaging of Cardiac Cell Transplantation in Living Animals Using Optical Bioluminescence and Positron Emission TomographyJoseph C. Wu, Ian Y. Chen, Gobalakrishnan Sundaresan, Jung-Joon Min, Abhijit De, Jian-Hua Qiao, Michael C. Fishbein and Sanjiv S. GambhirCirculation. 2003;108:1302-1305, originally published September 15, 2003http://dx.doi.org/10.1161/01.CIR.0000091252.20010.6E
Citation Manager Formats
Share this Article
- Molecular Imaging of Cardiac Cell Transplantation in Living Animals Using Optical Bioluminescence and Positron Emission TomographyJoseph C. Wu, Ian Y. Chen, Gobalakrishnan Sundaresan, Jung-Joon Min, Abhijit De, Jian-Hua Qiao, Michael C. Fishbein and Sanjiv S. GambhirCirculation. 2003;108:1302-1305, originally published September 15, 2003http://dx.doi.org/10.1161/01.CIR.0000091252.20010.6E
Related Articles
- No related articles found.
Cited By...
- Noninvasive Monitoring of Oxidative Stress in Transplanted Mesenchymal Stromal Cells
- Enlightened Stem Cells in the Heart: More Efficient and Safer Reporter Gene Imaging
- Cellular Immunogenicity of Novel Gene Immunogens in Mice Monitored by in Vivo Imaging
- A Strong Regenerative Ability of Cardiac Stem Cells Derived From Neonatal Hearts
- The potential of stem cell therapy for stroke: is PISCES the sign?
- Cell Tracking and the Development of Cell-Based Therapies: A View From the Cardiovascular Cell Therapy Research Network
- Structure-guided Engineering of Human Thymidine Kinase 2 as a Positron Emission Tomography Reporter Gene for Enhanced Phosphorylation of Non-natural Thymidine Analog Reporter Probe
- In vivo bioluminescence for tracking cell fate and function
- Cardiovascular Molecular Imaging: Focus on Clinical Translation
- Current Perspectives on Imaging Cardiac Stem Cell Therapy
- Assessment and Optimization of Cell Engraftment After Transplantation Into the Heart
- Reporter Gene PET for Monitoring Survival of Transplanted Endothelial Progenitor Cells in the Rat Heart After Pretreatment with VEGF and Atorvastatin
- Molecular imaging using contrast-enhanced ultrasound: evaluation of angiogenesis and cell therapy
- Noninvasive Quantification and Optimization of Acute Cell Retention by In Vivo Positron Emission Tomography After Intramyocardial Cardiac-Derived Stem Cell Delivery
- Molecular imaging of endothelial progenitor cell engraftment using contrast-enhanced ultrasound and targeted microbubbles
- Cardiovascular molecular imaging: an overview
- Combined Reporter Gene PET and Iron Oxide MRI for Monitoring Survival and Localization of Transplanted Cells in the Rat Heart
- In Vivo Imaging of Stem Cells and Beta Cells Using Direct Cell Labeling and Reporter Gene Methods
- Molecular Imaging: Antidote to Cardiac Stem Cell Controversy
- Comparison Between Adenoviral and Retroviral Vectors for the Transduction of the Thymidine Kinase PET Reporter Gene in Rat Mesenchymal Stem Cells
- Pilot Trial on Determinants of Progenitor Cell Recruitment to the Infarcted Human Myocardium
- Serial Noninvasive In Vivo Positron Emission Tomographic Tracking of Percutaneously Intramyocardially Injected Autologous Porcine Mesenchymal Stem Cells Modified for Transgene Reporter Gene Expression
- Multimodality Evaluation of the Viability of Stem Cells Delivered Into Different Zones of Myocardial Infarction
- Cardiovascular nuclear imaging: from perfusion to molecular function
- Molecular-Genetic Imaging Based on Reporter Gene Expression
- Imaging of VEGF Receptor in a Rat Myocardial Infarction Model Using PET
- Magnetic Resonance Imaging Overestimates Ferumoxide-Labeled Stem Cell Survival After Transplantation in the Heart
- Nuclear Cardiac Stress Testing in the Era of Molecular Medicine
- Comparison of Imaging Techniques for Tracking Cardiac Stem Cell Therapy
- A Human-Derived Reporter Gene for Noninvasive Imaging in Humans: Mitochondrial Thymidine Kinase Type 2
- Molecular Magnetic Resonance Imaging in Cardiovascular Medicine
- Role of Imaging in Cardiac Stem Cell Therapy
- Stem cell-related cardiac gene expression early after murine myocardial infarction
- Imaging Stem Cells Implanted in Infarcted Myocardium
- Cardiac Stem Cell Therapy: Need for Optimization of Efficacy and Safety Monitoring
- Collagen Matrices Enhance Survival of Transplanted Cardiomyoblasts and Contribute to Functional Improvement of Ischemic Rat Hearts
- Adenoviral Human BCL-2 Transgene Expression Attenuates Early Donor Cell Death After Cardiomyoblast Transplantation Into Ischemic Rat Hearts
- Regeneration Gaps: Observations on Stem Cells and Cardiac Repair
- In Vivo Visualization of Embryonic Stem Cell Survival, Proliferation, and Migration After Cardiac Delivery
- Mesenchymal Stem Cell Targeting of Microscopic Tumors and Tumor Stroma Development Monitored by Noninvasive In vivo Positron Emission Tomography Imaging
- Cellular MR Imaging
- In Vivo Detection of Stem Cells Grafted in Infarcted Rat Myocardium
- Molecular Beacons Illuminate Subcellular Events
- Development of a Sodium/Iodide Symporter (NIS)-Transgenic Mouse for Imaging of Cardiomyocyte-Specific Reporter Gene Expression
- Cardiac reporter gene imaging using the human sodium/iodide symporter gene
- In Vivo Tracking of Stem Cells for Clinical Trials in Cardiovascular Disease
- PET of Cardiac Transgene Expression: Comparison of 2 Approaches Based on Herpesviral Thymidine Kinase Reporter Gene
- Molecular Imaging of the Kinetics of Vascular Endothelial Growth Factor Gene Expression in Ischemic Myocardium
- Transplantation of cardiotrophin-1-expressing myoblasts to the left ventricular wall alleviates the transition from compensatory hypertrophy to congestive heart failure in Dahl salt-sensitive hypertensive rats
- Seeing Within: Molecular Imaging of the Cardiovascular System
This article has not yet been cited by articles in journals that are participating in Crossref Cited-by Linking.


text:
With just a few weeks left of the transfer window, the chance to secure many of Brazilian football's leading lights has passed, and instead clubs must be more picky in who they attempt to make a late move for.
Besides the five players we picked out last week as potentially being bound for Europe this month, here Bleacher Report takes a look at those whose situation may just allow for a late move.
Time is of the essence, as replacements will need to be found before the Brazilian window closes, but there is still time to get a transfer across the line.
Who, then, should clubs be looking at as possible August signings?