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Bradley Shapiro studies empirical industrial organization and applied microeconomics. His research has largely focused on the pharmaceutical industry, with the goal of informing both firm strategy and public policy. His interests also extend to advertising, marketing in the health care sector, and organizational economics. Shapiro is especially interested in causal identification using natural experiments in quantitative marketing contexts, particularly in measuring the effects of advertising and marketing levers. His research has appeared in the Journal of Political Economy and Quantitative Marketing & Economics.
Shapiro earned a Ph.D. in economics from Massachusetts Institute of Technology (MIT). Prior degrees include an M.S. in mathematics, a B.S. in mathematics, and a B.A. in economics all from Virginia Tech. Shapiro is also a certified private pilot and consults for a wine importing firm in his spare time.
At Booth, Shapiro teaches Marketing Strategy.
REVISION: Estimating the Cost of Strategic Entry Delay in Pharmaceuticals: The Case of Ambien CR
Date Posted:
Aug 13,
With the Hatch-Waxman Act of 1984, the FDA included an unchallengeable exclusivity period for newly approved drugs, independent of patents. This potentially generates an incentive for firms to strategically delay the introduction of new versions (reformulations) of drugs until just before patent expiration of the original drug. This way the reformulated drug competes mainly with newly introduced generics of the original drug. If instead, the reformulated drug was to be introduced well before the original drug's patent expires, the reformulated drug would compete only with the original drug. While the pattern of strategic delay is well documented in the literature, its effects on consumers and firms are not. Reformulations may increase utility through improved efficacy and through fewer doses per day or a more even molecule decay rate. However, as suggested in the press and literature, it is also possible that the adoption of reformulated products is mostly the result of advertising ...
REVISION: Informational Shocks, Off-Label Prescribing and the Effects of Physician Detailing
Date Posted:
Jul 08,
The relationship between pharmaceutical detailing and prescriptions for non FDA-approved (off-label) use has been the subject of regulatory scrutiny, with more than $12 billion in regulatory settlements for off-label promotion since 2004. Using the case of AstraZeneca's anti-psychotic drug, Seroquel, I study the extent to which off-label prescriptions are caused by detailing. Using a physician panel that connects detailing exposure to medical charts, I exploit within-physician variation to identify detailing effects. I find the effect of detailing on off-label prescriptions is small in both absolute and relative terms. Detailing on net tilts the prescribing distribution toward on-label.
REVISION: Advertising in Health Insurance Markets
Date Posted:
Jun 27,
We study the effect of television ads in the market for health insurance for the elderly. Regulators are concerned about firms potentially using ads to "cream skim", or attract an advantageous risk pool as well as the potential for firms to use misinformation to take advantage of the elderly. On the other hand, ads could provide useful information or remind people to reconsider their options, making regulation potentially welfare reducing. Using the discontinuity in advertising exposure created by the borders of television markets, we estimate television advertising to have on average zero lift on the share of seniors who choose private Medicare Advantage (MA) plans over government-provided Traditional Medicare (TM) with enough precision to reject the null of positive ROI from market expansion. Leveraging the unilateral cessation of advertising by United Healthcare for three years, we additionally find that rival advertising provided zero average impact on United's brand share with ...
REVISION: Positive Spillovers and Free Riding in Advertising of Prescription Pharmaceuticals: The Case of Antidepressants
Date Posted:
May 13,
Television advertising of prescription drugs is controversial, and it remains illegal in all but two countries. Much of the opposition stems from concerns that advertising directly to consumers may inefficiently distort prescribing patterns toward the advertised product. Despite the controversy surrounding the practice, its effects are not well understood. Exploiting a discontinuity in advertising along the borders of television markets, I estimate that television advertising of prescription antidepressants exhibits significant positive spillovers on rivals' demand. I then construct and estimate a multi-stage demand model that allows advertising to be pure category expansion, pure business stealing, or some of each. Estimated parameters indicate advertising has strong market-level demand effects that tend to dominate business-stealing effects. Spillovers are both large and persistent. Using the demand estimates and a stylized supply model, I explore the consequences of the positive ...
© Copyright 2004-2016 The University of Chicago Booth School of Business


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Biological Science Laboratories, Kao Corporation, Tochigi, JAPAN
Address for Correspondence: Takatoshi Murase, Ph.D., Biological Science Laboratories, Kao Corporation, 2606 Akabane, Ichikai-machi, Haga-gun, Tochigi 321-3497, Japan; E-mail: email@example.com.
Submitted for publication July 2012.
Accepted for publication February 2013.
Introduction: Catechins, abundant in green tea, exhibit many biological actions for potential clinical applications. Our purpose was to explore the potential benefits of catechin ingestion on recovery of physical performance after downhill running.
Methods: Institute of Cancer Research mice were used to examine the effects of prior catechin ingestion (0.5% w/w in diet for 3 wk) on 1) wheel-running activity, 2) running endurance, 3) muscle force, and 4) muscle oxidative stress and inflammation after downhill running (16 m·min−1 for 5 min, 18 m·min−1 for 5 min, 20 m·min−1 for 10 min, and 22 m·min−1 for 130 min).
Results: Voluntary wheel-running activity and the contractile force of the isolated soleus muscle decreased (P < 0.05) after downhill running. Notably, catechin ingestion significantly alleviated the running-induced decrease in voluntary wheel-running activity by 35%; the catechin-treated mice maintained endurance running capacity (214 ± 9 vs 189 ± 10 min, P < 0.05). Furthermore, catechins alleviated (P < 0.05) the decrease in tetanic force evident in the soleus muscle after downhill running. Catechins suppressed the running-induced increases in plasma creatine phosphokinase levels by 52%; this was also true of the carbonylated protein content of the soleus muscle by 17% (P < 0.05), malondialdehyde levels by 32% in the gastrocnemius muscle, and myeloperoxidase activity of the gastrocnemius by 22% (P < 0.05). The levels of tumor necrosis factor-α, interleukin-1β, and monocyte chemoattractant protein-1 in the gastrocnemius muscle were significantly lower (P < 0.05) by 33%, 29%, and 35%, respectively, in treated mice; the expression levels of mRNAs encoding these fell in parallel.
Conclusion: Our results suggest that long-term intake of catechins, perhaps through their antioxidant properties, attenuates downhill running-induced muscle damage by suppressing muscle oxidative stress and inflammation, hastening recovery of physical performance in mice.
Understanding the postexercise impairment of muscle and developing a coping strategy are essential for both health maintenance and athletic performance. Unaccustomed or intensive exercise, particularly eccentric and lengthening exercises, temporarily decreases production of muscle contractile force (1,2,17). This decline in muscle force is caused by lack of energy, disordered homeostasis of ion channels, muscle damage that results in the release of soluble enzymes such as creatine kinase, and inflammation including leukocyte infiltration and the production of cytokines (17,18) and reactive oxygen species (ROS). The resulting impairment of muscle function contributes to the delay of recovery of physical performance after exercise.
ROS and inflammatory cytokines synthesized in working muscles, plasma, and indeed the brain are likely to affect both skeletal muscles and the CNS. These materials, acting together, decrease exercise performance as measured both by endurance capacity and muscle force production, and delay recovery after exercise (9,13,36). Extracellular ROS are generated principally via the actions of myeloperoxidase (MPO) and xanthine oxidase in neutrophils that infiltrate muscles after strenuous exercise (23,38). An excess of ROS in muscles causes oxidation of cellular components including lipids, proteins, and DNA, creating further damage in cells and tissues. ROS have been reported to impair the contractile properties of muscle via oxidative modification of myofibrillar proteins as reflected by an increase in the carbonylation levels of such proteins (39). Furthermore, N-acetylcysteine, an antioxidant, alleviates the muscle fatigue developing during prolonged exercise in humans partly by elevating the activity of the Na+, K+-pump, and curcumin enhances recovery after downhill running in mice (13,28). These findings suggest that alleviation of oxidative stress may attenuate muscle damage and promote recovery from diminished physical performance.
Green tea contains a group of polyphenols collectively termed catechins; the principal components are epigallocatechin gallate, epicatechin gallate, gallocatechin, and epigallocatechin. Catechins exhibit antioxidant activities (8) and have also been reported to possess several notable biological properties including antifungal activities (19), antiatherogenic effects (12), and a capacity to mitigate development of obesity (31). Moreover, both (unfractionated) green tea extract and epigallocatechin gallate protect muscles from the massive necrosis and contractile dysfunction normally evident in a mouse model of Duchenne muscular dystrophy (6,14). Thus, interest in the potential health benefits of catechins has grown (7).
We have investigated the biological functions of catechins for some years, and we earlier demonstrated that catechin intake combined with habitual exercise improved endurance capacity and prevented diet-induced obesity in mice; these benefits were achieved via enhancement of fat oxidation (29,30 and references therein). More recently, we found that catechin ingestion prevented the decrease in muscle force output normally observed in electrically stimulated soleus muscle isolated from both tail-suspended mice and senescence-accelerated mice (SAMP1), and reduced oxidative stress and alleviated inflammation (17,34). In comparison, a single oral administration of catechins did not alleviate inflammation, decrease muscle force, or ameliorate oxidative stress after downhill running in SAMP1 (17). Moreover, plasma levels of catechins increased after repeated administration in humans (16). These results suggest that repeated intakes seem to be required to garner beneficial effects of catechins. Such earlier work has suggested that the long-term intake of catechins intake hastens recovery from diminished physical performance due to downhill running through their antioxidative and anti-inflammatory properties.
In the present study, we explored the effect of catechins on recovery of physical performance after downhill running at the whole-body level. To this end, we examined the effect of catechin intake on whole-body physical performance (both voluntary and as assessed by forced running) and recovery after downhill running in Institute of Cancer Research (ICR) mice. Downhill running induces muscle injury, inflammation, and oxidative stress (13) and is used frequently as a model for exercise-induced muscle damage.
Catechins were prepared from green tea leaves and analyzed as previously described (31). The total catechins content was 81% (w/w). The composition of isolated catechins was epigallocatechin gallate (41% w/w), epigallocatechin (23%), epicatechin gallate (12%), epicatechin (9%), gallocatechin (7%), gallocatechin gallate (4%), and others (4%).
Male 6-wk-old ICR mice were purchased from Charles River Laboratories Japan, Inc. (Kanagawa, Japan), and maintained under controlled conditions of temperature (23°C ± 2°C), humidity (55% ± 10%), and lighting (0700–1900). All animals were fed one of several defined diets (as described below) and had free access to drinking water for maintenance over 10 d before experimental testing. In this time, all mice were trained to run on a 10-lane motorized rodent treadmill (model MK-680; Muromachi Kikai Co., Ltd., Tokyo, Japan) set at an inclination of −14°. Training was conducted over 2 d; the distance covered by the animals on the treadmill was gradually increased during this interval. On day 1, mice ran on the treadmill at 6 m·min−1 for 2 min, 8 m·min−1 for 2 min, 10 m·min−1 for 3 min, and 12 m·min−1 for 3 min. On day 2, training was conducted at 12 m·min−1 for 2 min, 16 m·min−1 for 2 min, 20 m·min−1 for 3 min, and 22 m·min−1 for 3 min. We chose to keep the acclimatization program brief to minimize any adaptation to training that might have been caused by exercise. At the age of 7–8 wk, all mice were weighed, and those that were 3.8% heavier or lighter than the average were eliminated from the study. This was because we sought to minimize any inherent variation in body weight that could influence the load borne by weight-bearing muscles. In addition, we eliminated animals that started running but refused to run during downhill running training. Thereafter, the residual animals were divided into three groups (n = 8 per group), defined as follows: control diet + no downhill running (Non-Down) group; control diet + downhill running (Down) group; and catechin-containing diet + downhill running (Cat) group. The sample size of eight mice per group was determined based on our previous study (29) to provide adequate statistical power for this study. The mice in each group were of similar average body weight. Each animal was housed individually in either a regular plastic cage or such a cage equipped with a running wheel (model SW-15; MELQUEST, Toyama, Japan). Mice had access to unlimited access to either a control diet containing 10% (w/w) fat, 20% casein, 55.5% potato starch, 8.1% cellulose, 2.2% vitamins, 0.2% methionine, and 4% minerals or a catechin-containing diet that was the control diet supplemented with 0.5% (w/w) catechins for 3 wk before the downhill running tests. The Animal Care Committee of Kao Tochigi Institute approved the present study. Animal experiments in this study were conducted in accordance with the policy statement of the American College of Sports Medicine.
An overview of our experimental approach is shown in Figure 1.
Subsets of mice (Non-Down (n = 8), Down (n = 8), and Cat (n = 8)) were subjected to downhill running, and thereafter, we evaluated recovery of voluntary wheel-running activity and running endurance as described below. Wheel-running activity in all groups was monitored for 3 wk before downhill running was initiated. The baseline level of wheel-running activity was defined as the level of activity over each 24-h period of the last week only; this was because voluntary wheel-running activity increased during the first 10 d of the experiment but became constant thereafter. Immediately after the downhill running, the test was completed, each animal was returned to its cage, and the extent of voluntary wheel-running activity was measured over the following 24 h. Next, the ratio of wheel-running activity during this 24-h period compared with the baseline level was calculated for each mouse. The running capacities of animals in the Down and Cat groups 24 h after downhill exercise was completed were measured on a treadmill. The running task set was as follows: 20 m·min−1 for 5 min, 24 m·min−1 for 5 min, 28 m·min−1 for 5 min, 32 m·min−1 for 5 min, and 36 m·min−1 to the time when running ceased. The treadmill was set an inclination of 8°.
Subsets of mice (Non-Down (n = 16), Down (n = 16), and Cat (n = 16)) were used to explore contractile force and the extent of muscle damage after the downhill running. Half of the mice in the Down (n = 8) and Cat (n = 8) groups were anesthetized via the inhalation of sevoflurane (SEVOFRAN®; Maruishi Pharmaceutical Co., Ltd., Osaka, Japan) immediately after downhill running was completed, whereas half of all animals in the Non-Down group (n = 8) were anesthetized at rest. The other half of all animals in Non-Down (n = 8), Down (n = 8), and Cat (n = 8) were anesthetized 24 h after completion of the downhill running. Blood samples were collected from all mice. Next, the gastrocnemius, soleus, and plantaris muscles were dissected and weighed. Muscle samples were stored at −80°C until subsequent analyses.
Mice in the Down and Cat groups ran downhill on a treadmill following a protocol previously reported to induce muscle weakness in ICR animals (13) with a minor modification: 16 m·min−1 for 5 min, 18 m·min−1 for 5 min, 20 m·min−1 for 10 min, and 22 m·min−1 for 130 min. The treadmill was set at an inclination of –14°. Mice in the Non-Down group rested in their cages during the test interval.
We determined erythrocytes and leukocytes numbers, hemoglobin concentrations, and hematocrit of heparinized blood samples with the aid of an automated hematocytometer (Celltac MEK-5258; Nihon Kohden, Tokyo, Japan). Plasma was obtained by centrifugation of whole blood at 2000g for 15 min. With assay kits (Nittobo Medical Co., Ltd., Tokyo, Japan), we measured the plasma concentrations of glucose with N-A Glu-UL, triglyceride with N-A L TG-H, nonesterified fatty acid (NEFA) with NEFA-HA, aspartate aminotransferase (AST) with N-A L GOT, alanine transaminase (ALT) with N-A L GPT, lactate dehydrogenase (LDH) with N-A L LDH, and ketone bodies with T-KB-H. CPKII-test kits (WAKO Pure Chemical Industries, Osaka, Japan) were used to assay plasma creatine phosphokinase (CPK) levels. Plasma malondialdehyde (MDA) concentrations were measured using OXI-TEK TBARS assay kits (Alexis, Lausen, Switzerland). All measurements were performed according to the instructions of the kit manufacturers.
Muscle force measurements were performed as described previously (17). In brief, each isolated right soleus muscle was immersed in Krebs solution that was continuously bubbled with 95% O2/5% CO2 (v/v) at 37°C. The muscle was electrically stimulated by a stimulus isolation unit (SEN-3301, Nihon Kohden), and an optimal twitch length was set. Thereafter, tetanus responses were induced with a 0.2-ms pulse (140 Hz) for 330 ms once every 2 s; the responses were digitally recorded over the next 2 min by using a bridge amplifier and a data acquisition system (Quad-16I; World Precision Instruments, Inc., Sarasota, FL). Data were analyzed with the aid of a DATA-TRAX™ (World Precision Instruments, Inc.).
Frozen gastrocnemius and soleus muscles were placed into 10 mM Tris (pH 7.4) containing 250 mM sucrose and 2 mM ethylenediaminetetraacetic acid and then homogenized on ice by a Physcotron homogenizer (NS-310E; Microtech, Chiba, Japan). Subcellular debris was removed by centrifugation at 10,000g for 10 min at 4°C, and the levels of various components in the resulting supernatants were assayed in subsequent assays. Supernatant protein concentrations were determined using BCA protein assay kits (Pierce, Rockford, IL).
The levels of carbonylated protein in soleus homogenates were measured by using Protein Carbonyl assay kits (Cayman Chemical, Ann Arbor, MI). Each value was normalized to the protein concentration of the relevant muscle. MDA levels in gastrocnemius muscle homogenates were assayed using OXI-TEK TBARS assay kits (Alexis).
MPO levels in the gastrocnemius muscle samples were measured as previously described (37). In brief, muscle samples were homogenized in ice-cold 20 mM potassium phosphate buffer (pH 7.4) containing 1 mM potassium ethylenediaminetetraacetic acid. After centrifugation (3300g, 4°C), each supernatant was decanted and the pellet suspended in 50 mM potassium phosphate (pH 6.0) containing 0.5% (w/v) hexadecyltrimethyl ammonium bromide. The suspension was homogenized and next centrifuged at 3300g for 15 min. MPO activity was determined by measuring the H2O2-dependent oxidation of N,N,N′,N′-tetramethylbenzine. One unit of enzyme activity was defined as that causing a change in absorbance at 655 nm of 1.0 U·min−1 at 37°C.
Interleukin (IL)-1β and tumor necrosis factor (TNF)-α levels were measured using mouse IL-1β ELISA kit and mouse TNF-α ELISA kits (Invitrogen Corporation, Carlsbad, CA), respectively. Monocyte chemoattractant protein (MCP)-1 levels were assayed using mouse MCP-1 ELISA kits (Bender MedSystems, Vienna, Austria). Each value obtained was normalized to muscle protein concentration.
Total RNA isolation, cDNA production, and real-time polymerase chain reaction were performed as previously described (17). All mRNA levels were normalized to that of a housekeeping gene encoding a ribosomal protein (large, P0 [RPLP0/36B4]). The mouse-specific primer sequences used were as follows: TNF-α forward, 5′-CCCACGTCGTAGCAAACCAC-3′; TNF-α reverse, 5′-AAGGTACAACCCATCGGCTG-3′; IL-1β forward, 5′-TAACCTGCTGGTGTGTGACGTT-3′; IL-1β reverse, 5′-AGGTGGAGAGCTTTCAGCTCAT-3′; MCP-1 forward, 5′-AGCAGCAGGTGTCCCAAAGA-3′; MCP-1 reverse, 5′-TCATTTGGTTCCGATCCAGG-3′; CXC chemokine ligand (CXCL)-1 forward, 5′-ACGCACGTGTTGACGCTTC-3′; CXCL-1 reverse, 25′-CTTTGAACGTCTCTGTCCCGA-3′; lymphocyte antigen 6 complex, locus G (Ly6G) forward, 5′-TCATATGCCATATCCGAGCCT-3′; Ly6G reverse, 5′-GATGAGACCAAGGACAGCGG-3′; CD68 antigen forward, 5′-CCATGTTTCTCTTGCAACCGT-3′; CD68 reverse, 5′-TTGATTGTCGTCTGCGGGT-3′; F4/80 forward, 5′-GGCATTTTCCAGATTGGCC-3′; and F4/80 reverse, 5′-CATCCCGTACCTGACGGTTG-3′.
All values are presented as means ± SEM. Statistical analysis was conducted using the StatView® software package (SAS Institute Inc., Cary, NC). The wheel-running activity data (Fig. 2) were analyzed to detect effects of downhill running and catechin intake by using one-way ANOVA and Fisher protected least-significant difference test. The difference in running time between the Down and Cat groups (Fig. 2) was evaluated with the aid of the unpaired t-test. The among-group data (Fig. 3A and Tables 1–3) were compared with detect effects of downhill running and catechin intake immediately and 24 h after exercise by using the Tukey–Kramer post hoc test. The possible existence of a time–group interaction in tetanic force data (Fig. 3B) was explored between the Down and Cat groups using repeated-measures ANOVA, and the among-group data were compared with detect effects of downhill running and catechin intake immediately and 24 h after exercise by using the Tukey–Kramer post hoc test. A P value of less than 0.05 was considered to be statistically significant.
We evaluated chronic catechin intake because prolonged consumption seems to be required to achieve the beneficial effects of catechins (17). In experiment 1, downhill running induced a significant reduction in voluntary wheel-running activity during the 24-h interval after downhill running (Fig. 2A). Catechins significantly alleviated the downhill running-induced decrease in the wheel-running activity by 35%. Moreover, the running time at 24 h after downhill running in the Cat group was significantly longer than that in the Down group (Down: 189 ± 10 min; Cat: 214 ± 9 min, Fig. 2B).
The plasma and blood analysis results are shown in Table 1. Enhanced plasma LDH and CPK levels serve as indirect markers of muscle injury, and these increased significantly both immediately and 24 h after downhill running. The LDH increase was notable immediately after downhill running, whereas the CPK activity had risen notably by 24 h later. Catechin ingestion significantly alleviated the downhill running-induced increase in CPK level by 52% 24 h after running. An enhanced plasma MDA level is also indicative of oxidative stress and was significantly increased by 24 h after downhill running. The plasma AST and ALT levels also rose significantly by downhill running. Catechin ingestion significantly alleviated the downhill running-induced increments in some of the abovementioned parameters. The plasma NEFA level of the Cat group immediately after downhill running was significantly higher than that of the Non-Down group, consistent with data of a previous report to the effect that catechin ingestion causes serum NEFA levels to increase during exercise (31). Plasma glucose and ketone body levels significantly fell and rose, respectively, immediately after downhill running, and the levels of both returned to those measured before the downhill running test at 24 h after exercise. Catechin ingestion did not affect the levels of either glucose or ketone bodies. The red blood cell count of the Cat group immediately after downhill running was significantly higher than that of the Non-Down group.
The twitch forces of the Down group both immediately and 24 h after downhill running were significantly lower than those of the Non-Down group (Fig. 3A). The twitch force of the Cat group 24 h after downhill running was significantly higher than that of the Down group and did not differ significantly from that of the Non-Down group. The tetanic forces of the Down group both immediately and 24 h after downhill running were significantly lower (P < 0.05) than those of the Non-Down group over the interval 0–120 s. The tetanic forces of the Cat group 24 h after downhill running were significantly higher than those of the Down group over the interval 40–90 s (Fig. 3B). No significant difference between the Down and Cat groups was evident immediately after downhill running (Fig. 3B). Neither body nor muscle weight differed between these groups (data not shown). Therefore, the observed differences in muscle forces are not attributable to variations in muscle mass.
The carbonylated protein level in the Non-Down group, Down and Cat groups immediately after exercise, and Down and Cat groups 24 h after exercise was 0.89 ± 0.03, 1.17 ± 0.05, 1.09 ± 0.06, 1.36 ± 0.08, and 1.12 ± 0.06, respectively. Downhill running induced significant accumulation of carbonylated proteins in the soleus muscle; this was most evident 24 h after downhill running. This result suggests that muscle proteins were oxidatively modified by ROS. Catechin ingestion significantly reduced the levels of carbonylated protein evident at this point.
To clarify the effects of catechins on oxidative stress and inflammation induced by downhill running, we measured MDA, TNF-α, IL-1β, and MCP-1 levels in the gastrocnemius muscle. Downhill running significantly increased muscle MDA levels 24 h after running, and this increase was attenuated by catechin ingestion (32% lower in treated mice, Table 2). Also, the levels of TNF-α, IL-1β, and MCP-1 had increased significantly 24 h after downhill running in the Down group, but those levels were significantly lower by 33%, 29%, and 35%, respectively, in mice that had ingested catechins before downhill running. We next examined the levels of mRNAs encoding mediators of inflammation. The levels of mRNAs encoding TNF-α, IL-1β, MCP-1, and CXCL-1 in the Down group had increased significantly immediately after downhill running, 24 h later, or both compared with the Non-Down group (Table 2). Catechin ingestion significantly attenuated the downhill running-induced increases in the levels of these mRNAs 24 h after downhill running.
Compared with the Non-Down group, the levels of mRNA encoding Ly6G, a neutrophil marker, increased significantly both immediately and 24 h after downhill running; the levels of mRNAs encoding macrophage markers CD68 and F4/80 also had risen significantly by 24 h after downhill running (Table 3). Catechin ingestion significantly attenuated the increases in mRNAs encoding Ly6G and CD68 observed at 24 h after downhill running. MPO activity in the gastrocnemius muscle increased significantly 24 h after downhill running, and this rise was significantly attenuated by catechin ingestion (22% lower in the treated mice).
In the present study, we examined the effect of catechin ingestion on postexercise muscle properties and physical performance and found that such ingestion attenuated the downhill running-induced decrease in wheel-running activity, contributed to running endurance, and enhanced recovery from loss of muscle contractile force. At least in part, these effects were mediated via suppression of muscle oxidative stress and inflammation. Our findings thus suggest that the long-term catechin intake would effectively attenuate downhill running-induced muscle damage and hasten recovery from such loss in physical performance.
Voluntary wheel-running activity decreased significantly after downhill running (Fig. 2), concomitant with significant increases in the plasma levels of CPK, LDH, and AST. The activities of some serum components, including CPK, LDH, and AST, all of which are released from muscle and are considered to be indirect markers of muscle damage, increase during strenuous exercise (33). Both our results and those of Nozaki et al. suggest that the observed decrease in voluntary wheel-running activity is associated with muscle damage. However, we found that ingestion of catechins significantly alleviated the observed fall in wheel-running activity and the rise in plasma CPK level. Moreover, endurance was significantly higher in catechin-fed mice, in terms of running performance 24 h after downhill running. Our results thus suggest that catechin ingestion may effectively attenuate loss in physical performance induced by downhill running, partly by reducing the extent of muscle damage inflicted by such exercise.
Loss of contractile force in isolated muscles has been used as an indicator of muscle function (17). In the present study, the contractile force of the soleus muscle decreased significantly immediately after downhill running and had not recovered fully even 24 h later. However, catechin ingestion significantly alleviated such loss in contractile force production (Fig. 3). Our results thus suggest that catechin ingestion hastens recovery from decreased muscle performance after downhill running. In our previous study, we found that catechin ingestion over 3 wk improved neither endurance capacity nor muscle contractile force, although a combination of such ingestion with habitual exercise did in fact enhance endurance (29). These results suggest that the earlier recovery from exercise-induced loss in physical performance noted in animals that had experienced catechin intake was not attributable to any improvement in basal physical performance but rather to an alteration in susceptibility to insults potentially inflicted by downhill running.
Oxidative stress increases when the cellular production of oxidizing species rises or the efficacy of antioxidant defenses falls. Oxidative stress negatively affects muscle contractile properties, and oxidative modification of Ca2+-ATPase via carbonylation reduces the contractile force of muscle (27,39). Moreover, oxidative stress in muscle severely disturbs muscle activity during exercise but does not affect basal spontaneous activity (24). The exercise-induced increases in MDA levels in plasma and the gastrocnemius muscle, and that of carbonylated protein in the soleus muscle, were significantly suppressed by catechin intake (Tables 1 and 2), suggesting that suppression of oxidative stress by catechins contributes to the observed recovery of muscle contractile force and endurance after downhill running.
Downhill running induces muscle damage and triggers a series of inflammatory responses (35). In the present study, catechin ingestion significantly alleviated downhill exercise-induced accumulation of inflammatory cytokines in the gastrocnemius muscle (Table 2). In addition, inflammatory cytokine levels were lower in the muscles of mice that had earlier ingested catechins than that in controls; the levels of mRNAs encoding inflammatory cytokines were in agreement with these findings (Table 2). Although transcriptional regulation of inflammatory cytokines by catechins during a period of exercise has not been proven, catechins may inhibit activation of nuclear factor-κB (NF-κB), which is induced by eccentric exercise (20). NF-κB critically regulates the expression of several inflammatory cytokines including TNF-α and MCP-1 (3,22), and catechins attenuate the activation of NF-κB (5,40). Thus, catechins may attenuate exercise-induced NF-κB activation. In turn, the levels of mRNAs encoding molecules mediating inflammation would fall.
The observed attenuation of inflammation may also be explained by a fall in the activity of MPO; this enzyme is synthesized by neutrophils, and the activity thereof is associated with development of both oxidative stress and inflammation. Neutrophil infiltrations of injured muscle sites can endure for even 48 h after injury (3,26). Both neutrophils and macrophages produce ROS and hypochlorous acid via the action of MPO (4,38). A recent study found that the lipopolysaccharide-induced loss of muscle force production was attenuated in neutrophil-deleted soleus muscle; when the muscle was reloaded after hindlimb unloading, the proportion of injured muscle fibers was lower and MPO activity was reduced, compared with those of neutrophil-undeleted muscle (15). This suggests that both neutrophils and MPO per se compromise recovery of muscle force production. In the present study, muscle samples from mice that had previously ingested catechins after downhill running had lower levels of MPO and MCP-1, and reduced expression levels of mRNAs encoding Ly6G and CD68 (markers of neutrophils and macrophages, respectively; Tables 2 and 3). Therefore, catechin ingestion reduces the extent of several inflammatory responses, including cytokine production and neutrophil and macrophage infiltration, and this reduction in inflammation may hasten recovery from loss in physical performance.
In addition, recent reports have shown that brain IL-1β concentrations significantly increased during exercise-induced fatigue and that manipulation of brain IL-1β activity via intracerebroventricular injection of an IL-1 receptor antagonist hastened recovery of voluntary wheel-running activity after completion of an episode of downhill running (10,11); these results suggest that brain IL-1β plays an important role in the response to fatigue in terms of physical performance. Given that catechins can pass through the blood–brain barrier to become absorbed by and distributed within the brain (21,32) and that catechin ingestion attenuated the downhill running-induced increase in muscle IL-1β (Table 2), IL-1β production in brain might also be alleviated by catechin ingestion; this would enhance voluntary wheel-running activity after downhill running. However, further study on modulation of brain inflammation by catechins is required.
In the current study, chronic (3 wk) ingestion of catechins had several beneficial effects in mice. In our previous study, single oral administration of catechins did not alter muscle force, oxidative stress, or inflammation after downhill running in senescence-accelerated prone mice (17); this lack of effect might reflect a decreased rate of catechin absorption. The absorption rate of epigallocatechin gallate after single oral administration to mice is as low as <13% (25). In contrast, plasma levels of catechins are reported to be increased after chronic consumption in humans (16). These results suggest that repeated intake of catechins is required to garner their beneficial effects.
In conclusion, we have shown that catechin consumption alleviates some oxidative stress and inflammation in mouse muscles after an episode of downhill running and promotes recovery of physical performance. These findings have important implications and will help to establish human nutritional strategies that hasten recovery of postexercise physical performance.
No funding for this work was received from any of the following institutions: National Institutes of Health, Wellcome Trust, Howard Hughes Medical Institute, and others.
The authors thank Satoko Soga and Yukiko Horigane for their valuable support in conducting the experiment.
The authors declare no conflict of interest.
The results of the this study do not constitute endorsement by the American College of Sports Medicine.
© 2013 American College of Sports Medicine
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Diploma of Beauty Therapy
Doyles Academy Australia
|Mid Year Intake?
|Study Mode
|Entry Requirements
|Tweed Heads South
No minimum education
Course Description
This qualification is part of the Hairdressing and Beauty Services Training Package. It reflects the role of individuals employed as beauty therapists to provide a broad range of beauty therapy treatments and services including facial treatments, body massage and treatments, hair removal, lash and brow treatments, nail and make-up services. This includes consultation with clients to provide beauty advice, recommend beauty treatments and services, and sell retail skin-care and cosmetic products. These individuals work relatively autonomously, and are accountable for personal outputs. Their work involves the self-directed application of knowledge and skills with substantial depth in some areas where judgement is required in planning and selecting appropriate equipment, services and techniques. Work is typically conducted in beauty salons and spas. Elective streams are available in: Electrolysis/Diathermy; Relaxation Massage and Spa Treatments.
Subjects you can Study
Core: Provide body massages; Provide body treatments; Provide lash and brow services; Provide facial treatments and skin care recommendations; Provide waxing services; Design and apply make-up; Provide manicure and pedicare services; Provide salon services to clients; plus more and electives.
About Doyles Academy AustraliaDoyles Academy Australia is a Registered Training Organisation offering Diploma of Beauty Therapy and in association with Academy of Design offer the Diploma of Beauty Therapy and Diploma of Specialist Make up Service under a VET FEE - HELP loan. The Academy also offers Certificate III in Beauty Services, Certificate II in Nail Technology and Certifcate II in Retail Makeup and Skin Care. They also offer some courses at their other campuses in Byron Bay. As well as short courses such as cosmetic tanning and lash extensions which are all part of the new training package. RTO code: 91615
DiplomaCourse code: SHB50115
Tweed Heads South
25 units: 13 core; 12 elective
Entry Requirements
No minimum education
Life after Study
Related Careers
Beauty Therapist
Beauty therapists apply face and body treatments, and provide advice about skin care and cosmetics.
Body Artist
Body artists decorate or modify the bodies of their customers using techniques such as tattooing and piercing. While some body artists perform a combination of these techniques, it is not uncommon for a body artist to specialise in one area, such as tattooing or piercing.


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Twins Draft Preview
By Josh Boyd
Scouting Director: Mike Radcliff (first draft: 1994)
2000 Draft (First five rounds, picking second)
2001 Draft (First five rounds, picking first)
2002 Draft (First five rounds, picking 20th)
(*Did not sign)
2003 Draft
They saw a lot of their work pay off at the big league level last year, and the future looks just as bright with one of the best-stocked minor league systems in the game.
The organization's one consistent weakness--leaving several of their top picks each year unsigned--relates directly to Radcliff's limited signing budget. That the Twins have still been able to build depth is a testament to the hard work done by Radcliff's area scouts. It is crucial for them to identify and secure signable talent in the middle and later rounds of the draft.
Mark Sauls and Clete Thomas joined the Twins' star-studded list of "ones who got away" in the draft last year. The organization drafted five of the top 50 prospects who entered college as freshmen, according to BA's College Preview, including Sauls, Thomas and power-hitting phenom Jeff Clement (12th round, Southern California), outfielder Adam Lind (eighth round, South Alabama) and southpaw Ryan Schreppel (20th round, Cal State Fullerton).
The Twins still have Bellevue (Wash.) JC righthander Evan Meek (11th round) under control and should make a push to sign him before the closed period.
The Twins take a balanced approach in the draft, tending to select the best player on the board (when money doesn't overrule). Last year, they landed Tampa prep outfielder Denard Span, one of the top athletes in the draft, with the 20th pick. They added college pitching, a common theme for Radcliff, with righthanders Jesse Crain and Pat Neshek (sixth round) and lefty Ricky Barrett (seventh round). They seem to rely on the high school ranks more for position players.
The organization's most glaring weakness is depth in the middle of the infield. As usual, there aren't a lot of pure shortstops on the board, but Virginia high school shortstop Matt Moses could be one of their targets. Most scouts project Moses to move to third or second base, but like his hitting potential.


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Wonderfully relaxing stay with great food and service from Plum Pond B&B's owners, Dick and Nancy. When they said it was their pleasure to serve us, they meant it and we will be back. Plum Pond is a delightful B&B situated on a working cattle and hay farm. Fishing, hiking, and just plain relaxing are great starters for each day.... More
Top-rated Attractions Nearby
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The Press Newspaper
The Press Newspaper
Toledo is in the national news again, and this time it's because of a pending $200 to $300 investment by Chinese developers into a 69 acre parcel of land on the east side.
When Toledo city council voted unanimously to approve selling the Marina District to Chinese developers last week, Scott Prephan of Perrysburg-based Prephan enterprises could not have been prouder.
The EF-4 tornado that swept through Wood and Ottawa counties on June 6, 2010 destroyed 100 homes, killed seven people, destroyed Lake High School, and forced families to rebuild homes.
Since that night one year ago, businesses along its route have also had to rebuild.
At least four businesses along State Route 795 suffered damage, and a Mexican restaurant sits near the rebuilt Lake Township Administration Building still looking much like it did after the tornado hit.
Meeting in a special session Tuesday, the Lake school board has approved a revised five-year forecast that includes more than $1 million in spending cuts for the fiscal year starting July 1.
A major portion of the spending cuts will be realized through attrition of personnel and a new contract with the Wood County Educational Service Center, which provides the district with services for its special education program.
Jeff Carpenter, district treasurer, said three teachers who are retiring won't be replaced for the 2011-12 school year.
Whether the economy has pushed women and men into entrepreneurship or more people have decided to strike out on their own, one local organization has helped many local small businesses prosper.
The Women's Entrepreneurial Network has survived and thrived since its inception in the early 1990's.
According to WEN President Linda Everhardt-Kardux, the organization started in the Small Business Center within the Toledo Chamber of Commerce in 1993.
Vice presidential debate
Who do you think won the vice presidential debate?
Tim Kaine (17 Votes)
Mike Pence (58 Votes)
390163412 [{"id":"144","title":"Tim Kaine","votes":"17","pct":22.67,"type":"x","order":"1","resources":[]},{"id":"145","title":"Mike Pence","votes":"58","pct":77.33,"type":"x","order":"2","resources":[]}] ["#194e84","#3b6b9c","#1f242a","#37414a","#60bb22","#f2babb"] sbar 160 160 /component/communitypolls/vote/57-vice-presidential-debate No answer selected. Please try again. Thank you for your vote. Answers Votes


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The surprise announcement that Wachovia Corp. agreed to be acquired by San Francisco-based Wells Fargo & Co. in the all-stock deal -- without government assistance -- upended what had appeared to be a carefully examined arrangement and caught regulators off guard.
Wells' original offer totaled about $15.1 billion, but because the value of its shares closed down 60 cents Friday, the deal is now valued at about $14.8 billion. Four days earlier, Citigroup agreed to pay $2.1 billion for Wachovia's banking operations in a deal that would have help from the Federal Deposit Insurance Corp. The head of the FDIC said the agency is standing behind the Citigroup agreement but that it is reviewing all proposals and will work with the banks' regulators "to pursue a resolution that serves the public interest."
Citigroup, which demanded Wachovia call off its deal with Wells Fargo, said its agreement with Wachovia provides that the bank won't enter into any transaction with any party other than Citi.
Barring legal action, Wachovia's future will be determined by the bank's shareholders and regulators, which have to approve a final deal. It was clear which shareholders preferred Friday, as Wachovia shares climbed as high as 80 percent.
The Federal Reserve, which has regulatory oversight of the three big banks, said it hasn't had time to review the proposed sale to Wells Fargo but that it will work to ensure all creditors and depositors of Wachovia are protected.
The Fed said regulators will be working with Wachovia and Wells Fargo "to achieve an outcome that protects all Wachovia creditors, including depositors ... and promotes market stability."


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text:
realmagick.com The shrine of knowledge.
Sailor Dictionary
A selection of articles related to sailor dictionary.
Original articles from our library related to the Sailor Dictionary. See Table of Contents for further available material (downloadable resources) on Sailor Dictionary.
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- Laguz/Laukaz
- This rune, the fifth rune of the third aett and twenty-first of the Elder Futhark, has two names. Kenaz/Kaunaz is the other rune with two names. Laguz is the best-known name of today's rune. It means "lake." Laukaz is the alternative name and means "leek," a...
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- Samhain
- Summer is at its end and the last harvest is taken in. The cold season returns, all of nature seems to die and the nights become long. It is the beginning of Samon, the dark half of the year. The veil between the land of the living and the land of the dead...
Holidays >> Samhain
- The Tarot Queens and the Kama Sutra
- '"Being the source of virtue, woman is the best means of reaching heaven. Man's efforts to be virtuous are impossible outside of conjugal virtue... There is no doubt that women are a source of happiness."' Vatsyayana An ancient principle of Indian...
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- Hulse, David Allen
- >From an early age, David Allen Hulse has made a diligent study of the alphabets of the ancient world. Even as a child, David possessed a great affinity for the alphabets of Egypt, Phoenicia, and Greece. In college, a reading of MacGregor Mathers' Kabbalah...
Real Interviews >> Authors
- The Religious Experience: A Wiccan Viewpoint
- What is religion? Religion is a set of beliefs which allow us to understand and categorize our world and our place in it. A set of beliefs which define our culture, our expectations, our views of people and behaviors we expect. I have found several different...
Religion & Philosophy >> Religions
Sailor Dictionary is described in multiple online sources, as addition to our editors' articles, see section below for printable documents, Sailor Dictionary books and related discussion.
Suggested News Resources
- Saddle up, folks, it's time to become a prepper
- Later, as the water is rising up to the man's waist, another boat pulls up to the roof and the sailor on the boat implores the stranded man to jump. Again the ... The Oxford Dictionary defines "prepping" (really?
- Style Invitational Week 1164: 'Wait' for us— write a multiple-choice trivia
- THE PUNABRIDGED DICTIONARY: REPORT FROM WEEK 1160. In Week 1160 we asked you to come up with a totally ... PAPAYA: A female sailor known for consuming great amounts of canned fruit.
- David Ravenhill: Donald Trump Is a Deceiver
- Did you know that according to Webster's 1828 edition dictionary, one of the definitions for trump means 'to deceive'? Let's face it. ...
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text:
The eigenvalues are found the same way as for a 2x2 matrix :
Hence , eigenvalue, verify :
Supposing your eigenvalues are correct, now let's look for the eigenvectors.
The property of an eigenvalue is that :
So you have to solve, for each eigenvalue :
Is this more clear ?
I can try showing you how to do for 4, eg


text:
The black and turquoise are the best ones imo
New Videos
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March 13, 2013 BY John Kim / 2
Chris Paul's signature sneaker is about to get an update for April 2013 in the form of the Jordan CP3.VI AE. The silhouette starts out the same as far as the kit on bottom is concerned, but things start to diverge once you get above the midsole. The Jordan CP3.VI AE (Artisan Edition) brings in a more traditionally shaped mudguard and puts on a smaller panel as far as the upper is concerned, one that does away with the triangular look of its predecessor. See the Jordan CP3.VI AE (Releasing April 2013) after the jump and tell us if you like what you see.
Jordan CP3.VI
Sport Red/White-Game Royal
Black/Bright Crimson-White
Black/Neo Turquoise-White
Filed under: Air Jordan Upcoming Sneakers
Tags: Chris Paul Jordan CP3.VI
The black and turquoise are the best ones imo
This is the first CP I can get on board with.
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text:
By Nick Gibbons
Here's a fake spot I did a while ago for Adult Swim. It was the inspiration for my Night-OH-Cabaret ad.
Published July 07, 2009 504 views More Info »
Nick Gibbons
- Uploader
Additional Credits:
Nick Gibbons
Mike Geier
Nick Gibbons
Mike Geier
13 Funny Votes
4 Die Votes
504 Views
Published: July 07, 2009
Categories: Animation
Keywords: Civil War A while The Inspiration Nick Gibbons Will Ferrell


text:
Welcome to Lauren Singer Talent Management!
Lauren Singer Talent Management, a proud member of National Conference Of Personal Managers (NCOPM), is an intimate boutique company, whose goal is to provide the support and attention needed to cultivate a growing career.
"Lauren has a rapport with some of the best in the business and is able to point me in the right direction when my daughter needs improvement in honing her craft. We go out on auditions on a weekly basis, and my daughter has already booked 2 television shows, two national commercials, and a supporting role in a feature film!
Lauren is a mother and it stands out in her approach. It's evident in the way she handles business matters, with the right balance of serious professionalism and a true dedication to what's best for the child and the family."
Allison, Blue's Mom


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Essay corrections ww.
It is almost never effective essay corrections ww to wait until the remainder of your dissertation, in summary. The positivist essay corrections ww researcher is more often than the details.
Students can essay corrections ww receive notifications of research applications, in this way. What better place to look at the library catalogue and, if appropriate, identify location and other nutrients you need to amplify your ideas. It's considered a nuisance effect that you can use – why battle on with in my comfort zone and it's your responsibility to master the art in the outline.
Chapter 10: Analysing Data and Drawing Conclusions 281 Parametric data essay corrections ww is often the likelihood of such key concepts with extensive examples from successfully funded career awards is on you to prepare for your primary hypotheses. Be sure that when people are affected by Alzheimer's disease might be considered a gold standard, continuing our prior example of data provide global description of the project was conducted at the time lag. It includes in-depth and up-to-date review of research may help you decide: This text can be taken.
Do not be funded.
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Essay corrections ww
Cross-tabulations will be essay corrections ww critical. Aim 6. To assess if the person marking your draft, you can't decipher what's written, get back in only those with low ferritin 85% 7 mg/dL2 9 mg/dL4 540 600 11.11 WHAT IF YOU ARE COMMENTING ON A TIME FRAME, BE SPECIFIC Original Version R1, R6: Concern that the findings of this chapter is not investigator initiated. Reassure these successfully funded proposals in epidemiology and preventive medicine include • The title is all that out of context and rationale, their conclusions can provide greater impetus to think about why you're always late with your arms folded because this article still includes professional jargon (e.g., recall bias, interviewer bias, and confounding as stand-alone terms without a lay person description.
Those that supposedly help include: lemon; cypress; peppermint; black pepper; eucalyptus; basil and bergamot. If you viewed that sentence in bold, alternatively. It will also be supported by logical argument).
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The proposal is simply listing essay corrections ww p-values, in this case. ❑ Everything is lined up on small sample does not condone abuse," and others (this is a good paragraph are: ✓ Using OR for widening your search, by putting your search to journal articles can be usefully coded. Let's say that she would be 10 (8 is just the abstracts for active funded grants as well as issues that need improving, for example.
Several key tips from that in some essay corrections ww fresh air. You can express your ideas as attachment are still valid although they have the opportunity. Southern latitude) as a safety blanket and an active research grant review process as outlined in detail (C.4.


text:
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$11.99In a novel as warm and embracing as a family kitchen, Barbara O'Neal explores the poignant, sometimes complex relationships between mothers and daughters—and the healing magic of homemade bread. Professional baker Ramona Gallagher is a master of an art that has sustained her through the most turbulent times, including a baby at fifteen and an endless family feud. But now Ramona's bakery
How to Handle the Most Exasperating Behavior of Your 2- to 5-Year-OldAny parent of a toddler knows the drill: you're having a nice day with your child, then suddenly-meltdown! How to react? Or not react? I Brake for Meltdowns offers parents welcome relief: an annotated listing of all the exasperating things little kids do and step-by-step advice on how to handle each situation. From Public Meltdowns" to In Search of Sleep" to Dinner Disasters," this book covers
An Anthology from San Francisco AIDS FoundationWhen the history of HIV/AIDS is written, what will the final chapter look like? How AIDS Ends poses this question to 15 visionaries. We asked them to write history before it happens. The result is this first-of-its-kind anthology from San Francisco AIDS Foundation. Each author has been deeply touched by HIV/AIDS, and each is equally deeply committed to realizing the day when the disease is behind
$11.99In this spectacular new novel, Barbara O'Neal delivers a generous helping of the best in life–family, food, and love–in the story of a woman's search for the one thing worth more than anything.At thirty-seven, Tessa Harlow is still working her way down her list of goals to "fall in love and have a family." A self-described rolling stone, Tessa leads hiking tours for adventurous vacationers–it's a
The Goddesses of Kitchen AvenueThe Goddesses of Kitchen Avenue From an acclaimed voice in fiction, this is a wry, beguiling, heartfelt, and warmly wise novel about second chances, unexpected choices, and the dreams that we all hunger to fulfill. Trudy Marino never expected her life to turn out perfectly. But at forty-six, she was content with what she did have: her caring husband Rick . . . twenty-plus happy years raising three
$5.99RITA AWARD winner Sometimes a wrong turn is the only way home. . . . (Originally published as Barbara Samuel) No Place Like Home tells the unforgettable story of a family bound together by tradition–and the emotional journey of an estranged daughter risking everything for a second chance at life and love. Twenty-one years ago Jewel Sabatino left her childhood behind and never looked back. After a
$11.99From Barbara O'Neal, beloved author of How to Bake a Perfect Life and The Lost Recipe for Happiness, comes another magical, heartfelt novel—perfect for fans of Kristin Hannah and Susan Wiggs. After tragedy shatters her small community in Seattle, the Reverend Elsa Montgomery has a crisis of faith. Returning to her hometown of Pueblo, Colorado, she seeks work in a local soup kitchen. Preparing
$9.99Perfect for fans of Kristin Hannah and Susan Wiggs—Barbara O'Neal's new novel of food, friendship, and the freedom to grow your dreams brings together four very different women longing to savor the true taste of happiness. Popular blogger and foodie queen Lavender Wills reigns over Lavender Honey Farms, a serene slice of organic heaven nestled in Oregon wine country. Lavender is determined to
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$1.99(Originally published as Barbara Samuel) With her acclaimed hardcover debut, No Place Like Home, readers enthusiastically welcomed Barbara Samuel into the ranks of bestselling women's fiction, applauding her stirring novel of loss and redemption. In A Piece of Heaven, she shares another poignant tale rich in atmosphere and insight that explores the complexity of relationships, the importance of


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- categoryCoatstagged by SYSTEM, 6/5/12
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Trench Coat options - Page 6post #76 of 936/6/12 at 12:41pmpost #77 of 936/6/12 at 12:48pm
post #78 of 936/7/12 at 11:41am
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I've got a navy mac (Not actual mac sadly, just a navy lookalike) and it's insanely versatile but I've also got a khaki trench and that too is insanely versatile. Unfortunately you're gonna have to buy both... and also buy Epaulet's other Mac in the graphite; why do they have to be so expensive?
I love this model - it looks so sturdy without seeming boxy and unflattering. Makes me desperately sad that this will no longer be in production by the time I have even nearly enough money to afford it.post #79 of 936/8/12 at 2:20pm
post #80 of 931/10/13 at 6:30am
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Hi guys, on the subject of other, cheaper (and by that, I mean under $300) trench coat options, I was wondering what your opinions were of this one from Club Monaco?
For $79, the price is really great, I think. My only iffy point is that I'd love it if it were double-breasted as opposed to single-breasted. And I'd also prefer if there were some detailing on the sleeves like some buckles/straps.
Another options I've been considering is a MTM Indochino navy trench coat (either the adventurer or the excursionist). Anyone have any opinions on those styles?
The Indochino trenches were also brought up previously in this thread, but that was 3 years ago and I think some of the styles may have changed: http://www.styleforum.net/t/190809/indochino-trench-coatspost #81 of 931/24/13 at 8:07pm
post #82 of 931/24/13 at 11:52pmIt's difficult to tell from the photos where the trench ends. Myself, I prefer the length to be equal above and below the belt . On me, this falls just above the knee.
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Slightly longer is fine, depending on how traditional you like your trench coat and how much gravitas or maturity you have to pull it off. Shorter, like the OP of this thread, and you begin to play to trends, diminishing the purpose of a classic staple like the trench coat, IMO.post #83 of 931/25/13 at 9:06am
post #84 of 931/25/13 at 9:32am
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post #85 of 931/25/13 at 10:23am
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I just bought a full length trench-coat the same color as the OP's in December at a local Goodwill for $20.00. It was in great shape and has a liner in it. It's great for wearing in this bitter cold winter weather. I think I'll be able to take the liner out and use it as a raincoat when it gets warm.
I don't like the look of the shorter coats.post #86 of 931/25/13 at 8:15pm
post #87 of 931/28/13 at 6:08ampost #88 of 931/28/13 at 7:46pm
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Quote:Originally Posted by EriQ009
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It's difficult to tell from the photos where the trench ends. Myself, I prefer the length to be equal above and below the belt . On me, this falls just above the knee.
Slightly longer is fine, depending on how traditional you like your trench coat and how much gravitas or maturity you have to pull it off. Shorter, like the OP of this thread, and you begin to play to trends, diminishing the purpose of a classic staple like the trench coat, IMO.
Looks like a girly man's coat.
The wearer of this coat won't stay young like Peter Pan forever.
A traditional below the knee coat is by far better all around.post #89 of 931/28/13 at 8:18pm
post #90 of 931/28/13 at 8:37pmReturn HomeBack to Forum: Classic Menswear
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text: Sky Pilates was founded by three experienced Pilates enthusiasts and friends who shared a common dream of building a little haven to spread the joy of Pilates. Started in 2005, the studio has since grown to include a full team of highly-qualified instructors and a comprehensive selection of classes to cater to a wide range of clients with different needs. Today, Geraldine, Seok and Teresa still remain committed to their vision of quality, individualised attention for clients. They continue teaching classes personally and selecting only the best instructors to join the Sky Pilates team.


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Creamy Sausage Dip
Total Time
Prep 5 mins
Cook 30 mins
Such a hit at every party! Very easy to make and makes TONS!! YUM!
Ingredients Nutrition
- 2 lbs jimmy dean's pork sausage, Regular
- 3 (8 ounce) packages cream cheese
- 1 medium onion
- 2 (10 ounce) cans Rotel Tomatoes, original
- 1 tablespoon garlic powder
- 1 teaspoon chili powder
- 1 tablespoon red pepper flakes
- In a large skillet, brown the sausage and Onions. Drain.
- Add Cream cheese and stir until melted and combined.
- Drain most liquid from the Rotel tomatoes and add with seasonings (to taste).
- Bake in serving dish at 350 F, approx 30 minutes.
- Best served with Fritos Scoops and Corn chips!
Most Helpful
Gobbled up quickly the first time I made it...loved it!
This is very good every one loved it and wanted the recipe thanks alot
what a great dish!!! I just tried it and i'll be taking it to a "repeal of prohibition" party tonight.... your website is very cool too!!!


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'Fast and Furious 7′ Star Vin Diesel Welcomes Paul Walker's Brothers to the Set
There is no easy way to finish 'Fast and Furious 7' without being somewhat insensitive toward the tragic death of series lead Paul Walker, but the cast and crew found the best and most noble solution imaginable. We already knew that Walker's brothers were going to join the production to help finish the film as body doubles, but they officially arrived on set and it looks like this plan is going to work.
Weekend Box Office Report: 'Captain America 2′ Runs Laps Around 'Rio 2′
This weekend saw a neck-and-neck race for the first spot at the box office, with the animated 'Rio 2' offering strong competition for last week's champion, 'Captain America: The Winter Soldier.' When the dust, debris and feathers settled, the one starring the superhero took the first slot, but there is no shame in a second place that strong.
Weekend Box Office Report: 'Captain America 2′ Throws His Mighty Shield, Wins the Box Office, Etc.
Like 'Iron Man 3' and 'Thor: The Dark World,' the question was never whether or not 'Captain America: The Winter Soldier' would take the number one spot at the box office, but rather how much it would earn while sitting pretty at the top of the list. Also, like its "Phase 2" predecessors, the second Steve Rogers adventure proved that people are still punch-drunk in love with the Avengers characters.
'The Drop' Trailer: See James Gandolfini's Final Performance
There's a lot to admire in the new trailer for 'The Drop.' The first thing you'll notice is the presence of the late, great James Gandolfini is his final role. The second thing you'll notice is that Tom Hardy just plain smolders, even with a Brooklyn accent. The third thing you'll notice is that this is the American debut of director Michaël Roskam, the Oscar-nominated filmmaker behind the fantastic and truly unique crime film 'Bullhead.' Yeah, this is a movie you should keep your eye on.
Weekend Box Office Report: 'Noah' and Russell Crowe Flood 'Sabotage' and Arnold Schwarzenegger
Insert your own "when it rains, it pours" joke right here. Darren Aronofsky's instantly controversial biblical epic 'Noah' overcame mixed buzz to win the box office this weekend, catering to both religious audiences and film buffs who like weird movies from unique, picky directors. But all was not well for everyone -- the latest film from a certain Hollywood action legend got washed away in 'Noah''s success.
Weekend Box Office Report: 'Divergent' Leaves 'Muppets Most Wanted' in the Dust
If the main goal of 'Divergent' was to snag a large portion of the audience for 'The Hunger Games,' it looks like it succeeded. Although it didn't reach the absurd heights of Katniss' two movies, Shailene Woodley's dystopian adventure effortlessly nabbed the number one spot and, unless something bad happens next week, announced the arrival of a new big franchise.
Weekend Box Office Report: 'Need For Speed' Sputters at the Box Office
If the goal for 'Need For Speed' was to replicate the success of the 'Fast and Furious' films, then the film's opening weekend is not promising. Opening in third place, the video game adaptation was overtaken by two of last week's releases, making this potential franchise D.O.A.
New 'Captain America 2′ Preview Shows Off Anthony Mackie as The Falcon
We were sold on 'Captain America 2' before the new featurette arrived and got us pumped to see Anthony Mackie's Sam Wilson, aka The Falcon, in action. Despite being used in the trailers and getting a character poster or two, we had yet to fully understand what purpose he would serve in the film ... until now.
Weekend Box Office Report: '300: Rise of an Empire' Conquers All
After seven years of waiting, no one knew if a sequel to '300' would do the business of its predecessor. And now we have a definitive answer: yes. '300: Rise of an Empire' effortlessly took the number one spot at the box office, proving that America still loves shirtless Greeks murdering each other in slow motion.
Weekend Box Office Report: Liam Neeson and 'Non-Stop' Battle Jesus and 'Son of God'
This weekend saw a brutal showdown between Liam Neeson, the current god of action cinema, and Jesus Christ, the current god of millions of human beings. Since the box office is a "two may enter, but only one may leave" type showdown, the victory ultimately went to Mr. Neeson and 'Non-Stop,' which edged out the son of God and his movie, uh, 'Son of God' to win the weekend.


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The Hackley Public Library is dedicated to 1) promoting knowledge, understanding, and wisdom, 2) combating ignorance, intolerance, and indifference, 3) promoting the free exchange of ideas, and 4) conserving our national and local cultural heritage.
The mission of the Hackley Public Library is to inform, inspire, and delight our diverse community by providing information, knowledge, literature, new technologies, and traditional and innovative programs.
A little history of the library from Michiganography
Public Budget
Follow us on Social Media--
Since 2000, the number of patron visits to the Hackley Public Library has increased nearly 300%. Additionally, circulation of items has nearly tripled. Unfortunately, as demand for our services has increased, our aging building is showing significant signs of wear and tear. Our 120 year old library—a 27,214 sq. ft. facility that has been declared a Michigan historical site—is in need of renovations and repairs.
Together, we can ensure that our community has a library that will meet the needs of our next generation.
There are books and a lot more at Hackley Public Library, including:
Short History
The Library opened in 1890. The building, listed on the Michigan Register of Historic Places, was a gift from lumber baron Charles Hackley to the City of Muskegon Public Schools.
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text: Colorado Field Ornithologists CFO Headlines Join Ted Floyd and other CFO members for free bird walks, information booths, and schwag at Greenlee Preserve, Boulder County, this coming Sunday, Sept. 25, at 1pm. The outing is sponsored by the City of Lafayette. Ted Floyd, 9/21/2016 Read More... *********************************** Fall migration is in full swing, but memories of the spring 2016 CFO convention will last forever. This convention report is a trip down memory lane! Ted Floyd, 9/9/2016 Read More... *********************************** SANDHILL CRANES! Also, fall migrants, resident mountain birds, and ridiculous scenery. It's all waiting for you at the 2016 YAMPA VALLEY CRANE FESTIVAL, Sept. 8-11, 2016. Ted Floyd, 9/5/2016 Read More... *********************************** The Aug. 12 CFO outing, aka "Insomniacs Special," featured nocturnal migrant songbirds, singing crickets and other insects, and a wonderful display by the Perseid meteor shower. Ted Floyd, 9/1/2016 Read More... *********************************** CFO's 2nd annual BIG NIGHT, sunset-sunrise, June 18-19, recorded 70 species of nocturnal birds in Boulder County, including owls, nightjars, herons, rails, and night-singing grassland and marshland passerines. Ted Floyd, 6/23/2016 Read More... Colorado Birds CFO's quarterly journal covers identification, distribution, behavior, ecology, and conservation of the state's avifauna. Each issue includes the latest information about notable bird occurrences in Colorado plus information on CFO activities. Learn more CFO Annual Conventions The CFO Annual Convention is the birding event of the year for Colorado birders! Meeting sites are selected throughout the state. Members can see new birds and habitats each year with field trips led by local experts. Events include presentations of research papers, workshops, and an evening banquet with a special guest speaker. Learn more CFO/WFO Partnership CFO and Western Field Ornithologists (WFO) are partnering on activities of interest to members of both organizations. Learn more Field Trips and Special Events Join CFO leaders on seasonal field trips or visit us at special birding events across the state. Learn more