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Utility of DNA Sequencing for Direct Identification of Invasive Fungi From Fresh and Formalin-Fixed Specimens
Objectives: To describe and discuss the utility and potential pitfalls of ribosomal RNA locus sequencing for direct identification of invasive fungi from fresh and formalin-fixed, paraffin-embedded specimens.
Methods: DNA was extracted from fresh and formalin-fixed, paraffin-embedded tissue and subjected to real-time polymerase chain reaction (PCR) targeting ITS2 and D2 regions of fungal ribosomal RNA locus. Cycle sequencing was performed on PCR products, and the identity of sequences was determined using a public database.
Results: Four clinical cases of invasive fungal infection are presented to illustrate the utility of DNA sequencing for determining etiology when microbiological culture is negative, for shortening the time to identification of slow-growing fungi, for guiding antifungal therapy, and for shedding light on the pathogenesis of disseminated fungal infection.
Conclusions: Fungal ribosomal RNA locus sequencing from fresh or formalin-fixed, paraffin-embedded specimens is a powerful tool for rapid and accurate diagnosis of patients with culture-negative or uncultured invasive mycosis.
- Fungi
- Invasive
- Identification
- Sequencing
- Paraffin-embedded
Fungi are saprophytic eukaryotic organisms widely distributed in the environment.1 Except for dimorphic and dematiaceous molds, most fungi do not cause invasive disease in immunocompetent hosts. In fact, several species of yeast are considered normal human commensals.2 Conversely, many fungi behave as opportunistic pathogens in immunocompromised patients, causing rapidly progressing infections. Because invasive fungal infections (IFIs) can be life-threatening, especially in immunocompromised patients, it is critical that an accurate diagnosis be established early in the course of disease so that tailored antifungal therapy can be initiated.3 Conventional identification methods for fungi include microscopic visualization of the fungal element in fresh and/or formalin-fixed, paraffin-embedded (FFPE) specimens and isolation of the organism on fungal medium.4 Although microscopic examination may reveal clues about the identity of the fungus based on its size and shape, as well as the type of immunopathology present, morphologic identification is error prone.5 Definitive identification is accomplished by isolation of the organism on microbiological culture followed by phenotypic and genotypic testing of the isolate.4 In the absence of positive culture, ancillary tests such as serology and antigen assays may be helpful.6–8 Serology is widely used for the diagnosis of coccidiomycosis and histoplasmosis, but it lacks sensitivity, especially in patients with compromised humoral immune responses.7,8 Antigen tests also lack sensitivity in patients with localized infections as well as specificity due to cross-reactivity with other fungi.6,9,10
Clinicians may often be confronted with culture-negative or uncultured cases of IFI, thus necessitating empiric anti-fungal therapy. The wrong choice of antifungal agent, based on the incorrect interpretation of histopathologic findings, however, may lead to unnecessary morbidity and mortality.5 Therefore, culture-free methods capable of identifying invasive fungi directly from the specimen would be highly valuable. Pathogen-specific polymerase chain reaction (PCR) and in situ hybridization assays have been developed for the direct identification of invasive fungi,4,11,12 but the great phylogenetic diversity of fungi demands diagnostics with broader capabilities.4 Broad-range PCR coupled with amplicon sequencing has allowed direct detection and identification of bacteria from clinical specimens.13,14 In broad-range PCR, primers hybridize to highly conserved sequences and amplify the variable flanking sequences. Comparison of the target sequence with a database of known species allows identification to the species or genus level. The fungal ribosomal RNA operon encoding 5.8S, 18S, and 28S ribosomal subunit genes and the internal transcribed spacer regions (ITS1 and ITS2) is the most reliable and most frequently used locus for fungal identification by sequencing.15 Sequence-based identification of fungi from culture has proven to be more accurate than conventional methods.4,16–19
In this case series, we discuss the advantages and limitations of ribosomal RNA locus sequencing for direct identification of fungi from fresh and FFPE specimens.
Materials and Methods
The patients received their care at the Stanford University Medical Center between 2009 and 2011. Case summaries of patients are presented in Table 1. Approval by the Stanford University Institutional Review Board was not necessary for a small case series.
Fungal Sequencing Assay
Sequence-based identification of invasive fungi from fresh and FFPE specimens was validated in the Clinical Microbiology Laboratory of the Stanford University Medical Center. Testing of clinical samples was requested by the infectious diseases physicians.
DNA Extraction
For FFPE, five 10-μm sections were cut using a new microtome blade. The QIAamp DNA Mini Kit (QIAGEN, Valencia, CA) and EZ1 DNA Tissue Kit (QIAGEN) were used to extract DNA from fresh and FFPE specimens, respectively, in accordance with the manufacturer's protocols.
Amplification and Sequencing
Two separate real-time PCR reactions, one targeting 300 base pairs (bp) of ITS2 and another targeting 300 bp of the D2 region of the 28S gene, were performed on each sample. PCR was carried out in a 25-μL reaction mixture consisting of 0.5 μM of fungus-specific universal primers targeting ITS2 (ITS-3F, 5′-GCATCGATGAAGAACGCAGC-3′; ITS-4R, 5′-TCCTCCGCTTATTGATATGC-3′) or D2 (D2F, 5′-GWGACCGATAGCRMACAAGTA-3′; D1D2R, 5′-GCATATCAATAAGCGGAGGA-3′), 1× FastStart SYBR Green Master mix (Roche Applied Science, Indianapolis, IN), and RNase-free water. PCR was performed on a Rotorgene-6000 (QIAGEN). The PCR conditions included 95°C for 5 minutes, followed by 40 cycles of 95°C for 15 seconds, 58°C for 30 seconds, and 72°C for 35 seconds. The final step involved a 60°C to 95°C temperature ramp to generate a melting curve that was used to confirm the presence of amplicon with a melting temperature ranging from 80°C to 92°C. Cycle sequencing was performed with ITS-3F, ITS-4R, D2F, and D1D2R primers as previously described.20 DNA sequences were assembled with Lasergene software (DNASTAR, Madison, WI) and compared with those in the NCBI GenBank (http://www.ncbi.nlm.nih.gov/Genbank/) and CBS (http://www.cbs.knaw.nl/Medical/BioloMICSSequences.aspx). The following distance score criterion was used to identify fungal sequences: less than 1%, to the species level; 1% or more to 2% or less, to the genus level; and more than 2%, not identified. For Aspergillus and Trichophyton, a criterion of less than 0.5% was used to identify to the species level and 0.5% to 1% or less to the species complex level identification. Matching results for ITS2 and D2 regions were required.
Case Reports
Patient 1
A 44-year-old man with cystic fibrosis underwent bilateral lung transplantation in March 2010. His initial recovery was complicated by a sternal wound infection. A debridement specimen from the xiphoid tissue in June 2010 grew Aspergillus versicolor. The wound healed in response to combination therapy with caspofungin and voriconazole, followed by posaconazole. In December 2010, he presented with acute onset of bilateral lower extremity weakness and had extensive aortoiliac thrombosis and aneurysmal degeneration of the ascending aorta with mural thrombus. He underwent thrombectomy of the aorta and the external iliac arteries and stenting of the aortic pseudoaneurysm. The cultures from blood and the resected thrombus did not yield any growth, but histopathology showed numerous fungal elements. Fungal sequencing performed on the FFPE thrombus identified the organism as Aspergillus fumigatus. Given the discrepancy between A versicolor isolated from the sternal wound and A fumigatus identified from the thrombus, fungal sequencing was performed on the archived sternal tissue, and the fungus was identified as A fumigatus. Despite aggressive antifungal therapy, the patient's condition deteriorated, and he was transitioned to comfort care until death. Autopsy revealed an organized thrombus within the aortic stent that contained abundant fungal hyphae. His lumbar spine also had evidence of discitis and osteomyelitis containing fungal elements.
Patient 2
An 87-year-old white man from the Central Valley, CA, with a medical history of an aortic porcine valve placement in 1998 due to calcified aortic stenosis, presented in April 2009 with a 2-month history of fever, chills, night sweats, and weight loss. Blood and bone marrow cultures for non-fastidious organisms as well as mycobacteria and fungi were negative. Transesophageal echocardiogram showed a normal-appearing aortic porcine heterograft, and full-body computed tomography (CT) was unremarkable. Abnormal laboratory results included elevated alkaline phosphatase and transaminases. Liver biopsy specimens demonstrated granulomas, but no organisms were seen by histopathology, although culture was not performed. Coccidioidomycosis serology testing was negative, but an aberrant immunodiffusion band, representing a Histoplasma "M" band, was detected at the Coccidioidomycosis Serology Laboratory (Davis, CA). Histoplasma urine antigen testing was initially negative, but when repeated at the Mira Vista Laboratory (Indianapolis, IN) it was positive.
Despite antifungal therapy with itraconazole with sequential changes to voriconazole and liposomal amphotericin B, symptoms and laboratory tests normalized only after coadministration of prednisone. Antifungal therapy was discontinued in May 2010, but fever, weight loss, and antigenuria recurred in August 2010. Antifungal therapy with itraconazole was reinitiated, but fever and weight loss again failed to resolve until a short course of prednisone was administered. The patient developed congestive heart failure with moderate to severe aortic regurgitation in the spring of 2011 while still receiving itraconazole. The patient underwent aortic valve replacement in August 2011. Histopathology revealed many yeast forms of variable sizes Image 1A. Given the unusual course of his infection and lack of confirmatory diagnosis by culture, fungal sequencing was performed on the valve and identified the organisms as Ajellomyces capsulatus, the sexual form of Histoplasma capsulatum. Fungal culture of the valve grew H capsulatum after 5 weeks. Liposomal amphotericin B was continued postoperatively, with a transition to itraconazole to be continued indefinitely. The patient has had no evidence of active infection 14 months after valve replacement.
Patient 3
A 77-year-old asymptomatic immunocompetent man was noted to have several lung nodules on a chest x-ray screening examination required for enrollment into a retirement community. Subsequent evaluation with CT confirmed the presence of multiple calcified nodules in the both lungs measuring up to 10 mm in diameter. A CT-guided needle biopsy specimen of the nodules revealed necrosis with abundant yeast forms, but fungal culture was negative. A thorough infectious diseases workup was remarkable for positivity of Histoplasma total antibody. Histoplasma urine antigen was negative. To definitively identify the yeast, fungal sequencing was performed on FFPE tissue and identified the organisms as H capsulatum. Because the lung nodules were largely calcified and the patient was asymptomatic, a decision was made not to initiate antifungal treatment but to follow closely with imaging. A chest CT was repeated 3 months later and showed unchanged nodules. No treatment has been administered, and the patient remains asymptomatic to date.
Patient 4
A 27-year-old man with a history of ruptured arterial venous malformations (AVM) underwent emergent placement of an extraventricular drain (EVD) for cerebral hypertension. On hospital day 10, partial resection of the AVM was performed. Two days later, he developed a fever and seizures. The cerebrospinal fluid (CSF) cell count and chemistries were normal. Blood, CSF, and urine cultures were negative. Ceftriaxone was empirically initiated. On hospital day 14, the EVD was replaced because of a high suspicion that it was infected. On hospital day 18, the patient developed a new episode of fever and seizures. Cerebrospinal fluid was xanthochromic, with a leukocyte count of 58 cells/mm3 with 25% neutrophils, 14% monocytes, and 61% lymphocytes; an erythrocyte count of 2,790 cells/mm3; protein of 90 mg/dL; and glucose of 73 mg/dL. Gram stain revealed a moderate number of budding yeast, some demonstrating collarettes Image 1B. The CSF bacterial and fungal cultures remained negative. The patient was started on amphotericin B. Fungal sequencing performed directly on CSF identified the yeast as Malassezia restricta. Antifungal therapy was changed to oral voriconazole. The remaining portion of the AVM was subsequently resected and the EVD was removed. Three weeks after completion of voriconazole, the patient had no evidence of central nervous system infection.
Fungal sequencing is a powerful diagnostic tool for direct identification of fungi from fresh and FFPE specimens in patients with culture-negative invasive mycosis. Because the ribosomal RNA locus is in multiple copies (≥100 copies per genome) and contains diverse regions, targeting it maximizes the sensitivity of sequencing for fungal identication.4,21 Several studies have reported on the feasibility and performance of fungal sequencing for direct identification of fungi in specimens from patients with IFI.21,22 Sequencing was successfully completed on 97.3% of specimens when performed on fresh specimens infected with yeast compared with 63.2% to 70% when performed on FFPE specimens infected with various fungi.21,22 Lau et al21 reported successful identification of the fungi in 93.6% (fresh tissue, 96.8%; FFPE, 87.5%) and 64.3% (fresh tissue, 100%; FFPE, 54.5%) of culture-proven and solely histologically proven cases of IFI, respectively. These findings indicate that fresh tissue is superior to FFPE and suggest that not all fungal-like structures found in histopathology sections are real. Introduction of artifacts during staining can result in false-positive histology results. Although the specificity of fungal primer sequences prevents cross-priming of human DNA, contamination of the sample with commensal fungi or environmental spores could yield false-positive results and lead to mismanagement of patients.22
The cases presented here illustrate common clinical scenarios for which application of fungal sequencing can be helpful in illuminating the pathogenesis of IFI and in guiding antifungal therapy. In patient 1, sequencing results from an archived FFPE tissue section shed light on the pathogenesis of disseminated aspergillosis caused by A fumigatus that was incorrectly identified as A versicolor by phenotypic methods. Although the result did not have therapeutic implications in this case because the patient had renal insufficiency, important differences in antifungal susceptibilities have been reported across species of Aspergillus.23,24 In fact, while A fumigatus is generally susceptible to amphotericin B, A versicolor is most often resistant.25 Also, the ability to distinguish Aspergillus terreus or Aspergillus ustus from other Aspergillus species has important therapeutic implications, and thus correct identification of Aspergillus species could affect antifungal therapy and clinical outcome in such cases.26,27 In patient 2, evidence of systemic histoplasmosis had been primarily based on a positive Histoplasma urine antigen test, but the unusual course and extraordinarily slow response to antifungal therapy raised questions regarding possible cross-reactivity of the Histoplasma antigen test with another fungus.6 Thus, direct identification of H capsulatum from the excised prosthetic heart valve definitively established the diagnosis of histoplasmosis. This case also illustrates the advantage of sequencing to shorten the time to identify slow-growing fungi from weeks to days compared with conventional culture techniques.28 Because sequencing does not require the presence of viable organisms, it is also ideal for patients who have received antifungal treatment, as in patient 1, or in situations in which the entire specimen has been preserved in formalin for histopathology examination.13 In patient 3, sequencing from an FFPE biopsy section identified H capsulatum as the cause of pulmonary nodules in an asymptomatic elderly man. Because pulmonary nodules due to H capsulatum may not require treatment in an asymptomatic patient, the sequencing results determined that close follow-up was the most appropriate course in this case.29 In patient 4, the identification of the etiologic agent of EVD-associated meningitis due to M restricta was possible only by fungal sequencing directly from the CSF since this organism is not a known pathogen and does not grow on routine media.30 Sequencing may also be particularly useful for identifying invasive fungi growing in biofilms on foreign objects. Isolation of viable organisms from biofilms may be difficult due to the existence of organisms in nonreplicating states.31
Despite the many advantages of direct fungal identification with ribosomal RNA locus sequencing, infectious diseases practitioners must be aware of its limitations. First, the assay is not widely available because it has been developed and validated by only a limited number of clinical laboratories with expertise in DNA sequencing.32 Variables that are likely to affect the sensitivity and accuracy of the assay include amount of specimen obtained (open vs needle biopsy), fungal burden, fresh vs FFPE tissue, DNA extraction method, sequence targets, and primer selection.4,33 Second, sequence-based identification of fungi directly from specimens is subject to the same limitations that currently exist for identification of fungi from culture. Specific interpretive criteria for identifying fungi by DNA sequencing are currently lacking.4,34 Furthermore, the publicly available sequence database GenBank does not facilitate accurate identification of rare fungi due to incorrect nomenclature assigned to sequence entries, erroneous or truncated sequence entries, and incomplete taxon sampling of fungi-causing disease.19,35 Thus, when possible, sequence-based results should be correlated with phenotypic culture identification results and discrepancies resolved with additional genotypic and phenotypic testing.27 Third, not all fungi, including some clinically important fungi such as A fumigatus species complex and Fusarium solani species complex, can be identified using the ITS region.21 Accurate identification of these fungi requires sequence analysis of alternative genes.4 Fourth, because fungal identification by sequence analysis is highly susceptible to contamination with commensal fungi and environmental spores, it is imperative that testing is strictly limited to samples obtained from sterile sources with fungal elements present on the microscopic examination.21 Fifth, in the absence of positive culture, sequence results must be correlated with histopathology and ancillary test results (serology and antigen tests) to ensure the accuracy of sequence results.
In summary, when used cautiously, fungal ribosomal RNA locus sequencing from fresh or formalin-fixed specimens is a powerful tool for rapid diagnosis and management of patients with culture-negative or uncultured invasive mycosis.
Upon completion of this activity you will be able to:
describe limitations of conventional microbiological methods for detection and identification of fungi in clinical specimens.
discuss limitations of existing nucleic acid tests and serological tests for diagnosis of invasive fungal infections.
discuss advantages and limitations of fungal identification directly from clinical specimens by DNA sequencing.
describe specimen types that are most suitable for fungal identification by DNA sequencing.
The ASCP is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians. The ASCP designates this journal-based CME activity for a maximum of 1 AMA PRA Category 1 CreditTM per article. Physicians should claim only the credit commensurate with the extent of their participation in the activity. This activity qualifies as an American Board of Pathology Maintenance of Certification Part II Self-Assessment Module.
The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose.
Questions appear on p 276. Exam is located at www.ascp.org/ajcpcme.
We thank the staff at the Clinical Microbiology Laboratory of Stanford University Medical Center.
Funding: This study was supported by the Department of Pathology at Stanford University School of Medicine.
- © American Society for Clinical Pathology


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Oedipus rex fate and freewill essay
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text:
Pleco Chinese Dictionary
By Pleco Software
Open iTunes to buy and download apps.
Pleco is the ultimate Chinese learning companion - an integrated dictionary / document reader / flashcard system with fullscreen handwriting input and live OCR, from a company that's been making the world's best mobile Chinese learning apps since 2001.
Major features: ($ = paid add-on)
• Amazing dictionaries. We include two lovely free ones built-in: the popular open-source CC-CEDICT, with over 100,000 frequently-updated entries, and our own PLC dictionary, with 78,000 more detailed entries and over 20,000 example sentences (with Pinyin). More than two dozen other dictionaries are available as free and paid ($) add-ons, including licensed titles from well-known publishers like Oxford, Longman, FLTRP, Tuttle, SLPH, and Commercial Press.
• Powerful merged search: look up words by Chinese characters, Pinyin (spaces / tones optional), or English, with support for wildcards and full-text search. And no need to tediously flip between all of those dictionaries to find a word - just type in your search term and instantly get a combined, frequency-sorted result list.
• Links and cross-references: easily look up character components, break down words into individual characters, look up lists of words containing a particular character, search across all dictionaries for example sentences containing a word, and even find synonyms / antonyms using our (experimental) free thesaurus add-on.
• Gorgeous typography: see it for yourself in our screenshots - we think we're one of the best-looking Chinese dictionaries around. Alternate Kai/Song/Xing-style Chinese fonts are also available ($).
• Mainland/HK/Taiwan-friendly: every part of Pleco supports both traditional and simplified characters, headword pronunciations can be shown in Zhuyin as well as in Pinyin, and we even support Cantonese search and display in our PLC dictionary.
• Audio: Hear Mandarin and Cantonese pronunciations for words and example sentences using iOS' free built-in text-to-speech system. We also offer add-ons ($) with native-speaker Mandarin and Cantonese recordings, plus higher-quality Mandarin text-to-speech system options.
• Document Reader: bring in text from the clipboard and tap on words in it to instantly look them up. Upgrade ($) adds support for tap-lookups in text, HTML, DOC, DOCX, PDF, and EPUB files, and in embedded lyrics in songs and podcasts.
• OCR: look up words simply by pointing your device's camera at them, or by tapping on them in a still image. ($)
• Fullscreen handwriting: use your device's whole screen instead of just a little box. Extremely accurate and highly tolerant of stroke order mistakes. ($)
• Flashcards: create a card from any dictionary entry with a single button tap, import premade word lists, use advanced memorization techniques like SRS, and study in a variety of modes including fill-in-the-blanks handwriting and tone drills. ($)
• Stroke Order Diagrams: animations showing you how to draw each character; 500 characters for free, 28,000 in paid ($) version.
• User dictionaries: create new dictionary entries right on your device as you encounter new words. ($)
• Absolutely no ads.
We also offer famously excellent customer support and an active user community at http://www.plecoforums.com. And we're constantly working to make our products better; our sales and customer base have been growing for 15 years across 4 different mobile platforms. You can be confident that we'll still be fixing bugs and adding features for years to come.
Our paid add-ons can be transferred to other devices that link to your iTunes account just like any other paid iTunes content; simply install our free app, open up the "Add-ons" screen, and tap on the "Restore Purchases" button to activate your purchase on each device.
Please contact us at firstname.lastname@example.com, via the form at http://www.pleco.com/contact.html, or using the in-app "Contact Support" command with any questions - we'll be delighted to hear from you.
What's New in Version 3.2.14
• Added new experimental Kai-style stroke order diagrams. These are free for anybody who owns (or purchases) our current Stroke Order Diagrams add-on; go into Registration / Check for other purchases to activate them and you should then see them as available to download in Add-ons / Purchased but not Downloaded (or Features). Toggle between those and the existing Song-style stroke order diagrams by tapping on the button at the bottom left corner of the stroke order box.
These are "experimental" because they only cover a subset of the characters our full stroke order diagrams do + have not been as exhaustively checked; they come from the open-source Make Me A Hanzi project. We are aiming to have our own set of Pleco-developed Kai-style stroke order diagrams available sometime in 2017, with greater character set coverage, thorough checking against official stroke order standards, and some nifty new features like Taiwan-specific style / stroke order variations.
• Added an option in Settings / Search Interface to hide the voice recognition button.
• Added an option for our experimental OCR system to force it to use the telephoto camera on the iPhone 7+, as we have not yet managed to get automated switching between cameras working reliably.
• Added taptic feedback to the radical and dictionary screen slider bars ('show dict slider' in Settings / Definition Screen) on the iPhone 7 and 7+. We're working to add taptic feedback in other places, but we're trying not to get carried away; this seemed like a good place to do it first since it's consistent with other parts of iOS (sliders in contacts e.g. likewise provide taptic feedback).
• Fixed a bunch of bugs, including an audio dropout bug when using voice recognition and crashes when using English text-to-speech on pre-iOS-10 devices and when trying to open Lyrics Reader on iOS 10. (we have not yet found a way to fetch Apple Music lyrics, however)
Be sure to check out our experimental new OCR system - Settings / OCR / New OCR System - with a completely revamped motion detection system that should feel much less jittery than before. Also, we're looking for beta testers for our new recognition engine - email firstname.lastname@example.com or post to our forums if you're interested in that.
This will probably be our last update that supports iOS 8; we plan to do a few more minor updates with some helpful new features before our big 4.0 update, but those updates will likely all require iOS 9. Every device that can run iOS 8 can also run iOS 9 (and most people find iOS 9 is faster / more stable), so if you haven't updated yet we'd encourage you to do so soon.
As always, since the App Store resets our review average with every update, we'd really appreciate it if you'd leave another review - don't have to write anything, just the star button will do. We hate the fact that other developers put up nag screens asking for reviews, and would never do that ourselves, but reviews help us show up more prominently in search results, which leads to more users / more money to improve Pleco.
- Free
- Category: Reference
- Updated: 26 September 2016
- Version: 3.2.14
- Size: 121 MB
- Language: English
- Seller: Pleco Software
- © 2016 Pleco Software Incorporated and its licensors
Compatibility: Requires iOS 8.1 or later. Compatible with iPhone, iPad, and iPod touch.


text:
July 22, 2013
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I am a musician, writer, computer scientist and engineering organization manager. I am 60 years old and have been playing piano since I was 8. I graduated BS in Communications and MS Comp Sci. I have been writing all my life. I am published.
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Get an overview of this hotelFamily-friendly hotel with restaurant and 24-hour fitness center
Collect 200 points
Simply enter your Nectar card number when prompted. Your points will be credited 30 days after you return from your trip.*Offers may apply
This family-friendly Frankfurt hotel is within 1 mi (2 km) of The Squaire and Lufthansa Flight Training Center. Ruesselsheim City Park and Opel Villas are also within 9 mi (15 km).
Hotel Features
A restaurant, a 24-hour fitness center, and a bar/lounge are available at this hotel. WiFi in public areas is free. Other amenities include a sauna, a 24-hour business center, and self parking.
Room Amenities
All 249 soundproofed rooms feature WiFi and MP3 docks. Guests can enjoy deep soaking tubs, and other standard amenities include 24-hour room service, wired Internet, and LCD TVs.Information missing or incorrect? Tell us!Opens in a new window
Hilton Frankfurt AirportThe Squaire Am Flughafen Frankfurt HE 60549
Hotel Amenities
Hotel Amenities
Hilton Frankfurt Airport features a sauna and a 24-hour fitness center. The hotel offers a restaurant. A bar/lounge is on site where guests can unwind with a drink. High-speed wireless Internet access is complimentary.
Business-related amenities at this 4.5-star property consist of a 24-hour business center and meeting rooms. Event facilities measuring 18000 square feet (1672 square meters) include conference space. This family-friendly hotel also offers multilingual staff, coffee/tea in a common area, and concierge services. Onsite parking is available (surcharge).
- 24-hour front desk
- Babysitting or childcare (surcharge)
- Babysitting or childcare
- Breakfast available (surcharge)
- Coffee/tea in common areas
- Concierge services
- Dry cleaning/laundry service
- Elevator/lift
- 24-hour fitness facilities
- Free WiFi
- Total number of rooms - 249
- Number of floors - 11
- Porter/bellhop
- Luggage storage
- Conference space size (meters) - 1672
- Self parking (surcharge)
- Conference space size (feet) - 18000
- Bar/lounge
- Free newspapers in lobby
- Conference space
- Express check-out
- Meeting rooms 5
- Multilingual staff
- Restaurant
- Number of meeting rooms - 5
- Year Built 2011
- 24-hour business center
- Sauna
- Number of buildings/towers - 1
- Steam room
Family Friendly Amenities
- Family Hotel
- Free Wi-Fi
- Soundproofed rooms
- Babysitting or childcare (surcharge)
- Cribs/infant beds (complimentary)
Available in all rooms: Wireless internet access ( EUR 27.00 per 24-hour period ), Wired internet access ( EUR 27.00 per 24-hour period )
Available in some public areas: Free WiFi
Self parking (surcharge)
Room Amenities
- WiFi (surcharge)
- Connecting/adjoining rooms available
- Pay movies
- Soundproofed rooms
- Air conditioning
- Phone
- Daily housekeeping
- Bathrobes
- In-room massage available
- Slippers
- Deep soaking bathtub
- Iron/ironing board
- In-room climate control (air conditioning)
- Minibar
- Coffee/tea maker
- Satellite TV service
- Private bathroom
- Free toiletries
- Hair dryer
- Separate bathtub and shower
- Free cribs/infant beds
- Desk
- Blackout drapes/curtains
- MP3 docking station
- Wired high-speed Internet access (surcharge)
- In-room safe (laptop compatible)
- Room service (24 hours)
- Pillow menu
Where to Eat
Buffet breakfasts are available for a surcharge and are served each morning.
Restaurant Rise - This restaurant serves breakfast, lunch, and dinner. A children's menu is available.
The Fifth, Lounge and Bar - This bar serves light fare only.
24-hour room service is available.
Nearby Things to Do
Recreational amenities at the hotel include a sauna and a 24-hour fitness center.
To make special requests for accessibility needs, please note them at check-out when you book your room.
- Accessible bathroom
Hotel Policies
Check-in time starts at 2 PM
Minimum check-in age is 18
Check-out time is noon
Payment types
- Pets not allowed
You need to know
Extra-person charges may apply and vary depending on property policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
Optional extras
- Fee for buffet breakfast: EUR 33 per person (approximately)
- Fee for in-room wireless Internet: EUR 27.00 per 24-hour period (rates may vary)
- Fee for in-room high-speed Internet (wired): EUR 27.00 per 24-hour period (rates may vary)
- Self parking fee: EUR 32 per night
- Rollaway bed fee: EUR 50 per night
Hotel Name
- Frankfurt Airport Hilton
- Hilton Frankfurt Airport
- Hilton Hotel Frankfurt Airport
We should mention
One child 17 years old or younger stays free when occupying the parent or guardian's room, using existing bedding. The property has connecting/adjoining rooms, which are subject to availability and can be requested by contacting the property using the number on the booking confirmation.


text:
Download medical manager workbook full file cjag free
--Publisher's Description--
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Designed for use by participants in group cognitive behavioral therapy sessions on anger management for people with substance abuse problems or mental illness.HEIA Version 2.0 (Spring 2012) The Health Equity Impact Assessment (HEIA) Tool and Workbook were updated by the Ontario Ministry of Health and Long-Term Care …Medical Home - Practice-Based Care Coordination This workbook is designed to support practice-based quality improvement teams in their efforts to build …How AHIMA Moves You Forward. AHIMA's primary goal is to provide the knowledge, resources and tools to advance health information professional practice and standards ...Helps clinicians teach anger management techniques in a group setting to clients with substance abuse problems or mental illness. Uses cognitive behavioral therapy to ...This Student Workbook contains exercises to reinforce what you will learn in both Emergency Medical Responder, Fifth Edition and the classroom.2. Case Management DMAHS Definition Case management, a component of care management, is a set of activities tailored to meet a member's situational health …The Medical Transcription Workbook HEALTH PROFESSIONS INSTITUTE SECOND EDITION Professional Issues Anatomy & Physiology Medical Terminology SurgeryDepartment of Health and Human Services Centers for Disease Control and Prevention Office of Public Health Preparedness and Response PUBLIC HEALTH WORKBOOKThe Cognitive Behavioral Workbook for Weight Management: A Step-by-Step Program: Michele Laliberte, Randi E. McCabe, Valerie Taylor: 9781572246256: Books - …
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Alpine INE-W957HD Porsche 996 911 & 986 Boxster Double DIN Conversion Radio
Price reflects dash kit & other required parts
Brand: CAI
This is a complete radio replacement, hardware kit & Instruction Manual for select 996 911s & 986 Boxsters. This is the premium option for this generation of Porsche and includes a 7" LCD screen on the radio (largest available)
Kit Includes an Alpine INE-W957HD Navigation/Radio
1999 - 2004 911
1997 - 2004 Boxster
This kit allows you to either replace an existing Porsche factory Navigation unit, or to convert a factory single disc player to the larger navigation unit. All required parts are included in the kit.
Note: In '97 - '02 generation of vehicles the factory amplifier (if present) works with this kit and does not need to be replaced or bypassed. The harness that we supply with this kit allows full integration with both Bose and non-Bose equipped cars. DSP equipped vehicles with a single din radio are also compatible, though the DSP controller must remain connected (will not be operable) and can be stuffed in the dash behind the HVAC control plate.
Note: PCM (factory double DIN) equipped vehicles with DSP of all years require a replacement amplifier when upgrading the radio. The factory DSP amplifier is not compatible with aftermarket radios. You can identify a DSP equipped vehicle by looking at the amplifier in the trunk. It will have DSP written on it.
Note: 2003 and later models use a fiber optic audio system that can not be used with aftermarket radios. A replacement amplifier is required. We have several replacement amplifier kits available.
Installation is relatively straight forward if you follow the included instructions and have basic mechanical skills tools and are comfortable with making electrical connections.
This kit is designed for the DIY and professional alike. Free 1 (800) technical support comes with this product.
The kit includes:
- The radio: full featured Alpine INE-W957HD Double Din DVD with GPS Navigation and handsfree Bluetooth built in.
- Custom 996/Boxster Dash Trim Bezel
- Porsche Relocation Bezel for HVAC Controls
- Porsche cubby/pocket to replace existing CD/Cassette trays
- Radio to Vehicle Harness, prewired for your car
- Antenna Adapter
- HDMI Input
- Factory radio removal keys
- Porsche Crest Pre-loaded on Start Up Screen
- Full Installation Instructions
- Phone Tech Support Included
Genuine Porsche Climate Control Relocation Parts
The radio included in this kit is the top of the line unit that is loaded with modern features.
- iPod, iPhone, iTunes Tagging, and Video Playback Including Netflix
- Pandora (works with both iPhone as well as Android!)
- HD Radio
- USB for Mass Storage Devices
- Auxiliary Audio & Video Input
- GPS Navigation
- NAVTEQ Live Traffic Data
- Hands Free Bluetooth for Cellphone
- Bluetooth Audio Streaming
- 6 Channel Preouts
- Graphic Equalizer
- High/Low Pass Digital Filters
- Digital Time Alignment
- 1 Year Warranty
This Radio is also capable of additional accessories:
We have installed many different radio variations over the years and have found this one to be an absolute home run. The feature set and high level of quality that this radio offers makes it a perfect fit for these cars. If you have any questions or need any more information about this product or additional options feel free to give us a call. We are always happy to assist.
1(800) 892-6430
Shipping Time: This product is "made to order" and is custom made for your particular vehicle. This package typically ships within 2-3 business days of order


text:
Greens vote not to tolerate antisemitism
Delegates at this year's Green Party conference have backed a motion stating that the party did not tolerate or endorse antisemitism, but only after radically cutting the original resolution.
The original motion said that antisemitism also included elements of anti-Zionism and called for the Greens to adopt the European Union's definition of antisemitism.
It also stated that Israel's right to exist was consistent with the Green Party's policies and called upon all party members to condemn antisemitism, when forced to share a platform with other speakers who express antisemitic views.
The motion caused some opposition within the party, which this week voted to appoint MEP Caroline Lucas as its first leader. Ms Lucas has in the past been a staunch supporter of the boycott of trade with Israel and chaired a session at a Palestine Solidarity Campaign congress.
An opposing motion was tabled saying that the Greens have "engaged" with groups supporting the Palestinians and that Greens dissociate themselves from these groups' wider agenda "specifically if it reflects any implication of antisemitism".
The conference finally pared down the original motion. Councillor Chris Fox, one of those behind it, said: "It's a very small progress, but it's important that at least for the first time there is something on antisemitism in the Greens' policies."
The conference also featured a fringe event on "Anti-Zionism - a Jewish perspective." This was organised by Green Left and the PSC. A second fringe meeting discussed whether "criticism of Israel can be antisemitic."
Wed, 09/10/2008 - 12:58
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0 points
This piece is a bit misleading - I was there and involved, so I can explain. Pfeffer writes: "The original motion said that antisemitism also included elements of anti-Zionism and called for the Greens to adopt the European Union's definition of antisemitism." It didn't - it was very careful not to attempt to restrict or define. It said: "Antisemitic remarks and behaviours may include a variety of imagery, caricatures and references to global Zionist conspiracies etc." and "The EU's working definition of antisemitism shall be considered when determining what counts as antisemitism." http://www.greenparty.org.uk/files/conference/2008/Autumn_2008_final_age...
Thu, 09/11/2008 - 10:03
Rate this:
0 points
Caroline Lucas is an avowed enemy of Israel and is planning to be one of Brighton & Hove's three MPs after the next General Election. She is also viciously skilled at underhand debating and will use every trick in the book to seem to win a argument. Typically liberal-minded interviewers are putty in her hands because she can behave so charmingly to them and the arguments of her opponents can be put down with such jibes as that they are against her 'because she is a woman'. Mostly though, Lucas just refuses to reply to good arguments against her ideas and just keeps repeating the same points, faster and louder until the time allotted for debate is over. She then smiles sweetly and goes off, totally convinced of her her own moral superiority. On foreign policy Caroline Lucas has much in common with George Galloway. Needless to say, when the time comes to vote in the General Election, nobody in Brighton should vote for Caroline Lucas and the Greens.


text:
Discipline: Science
Copyright: 2009
Grade(s): 6 - 8
Program Type: Core
Delivery Method: Print
Lexile Level: 830-900
Updated content! Concepts and Challenges now includes the most current information on: the new nutrition pyramid, the eight-planet solar system, and Hurricane Katrina and the 2005 Tsunami.
New Components! Enhance your classroom with Discovery Channel DVDs created especially for Pearson. The Science Teacher Tech Pack helps with instruction by providing test banks, PowerPoint presentations, more than 1,400 virtual labs and more.
This comprehensive hardcover program offers the right balance of challenging content and text accessibility that helps all levels of students succeed in science. A unique left-hand "Concept" page and right-hand "Challenge" page make each lesson accessible and provide frequent review and reinforcement to build student confidence.
Concepts and Challenges provides opportunities for inquiry-based learning, visual learning, reading support, and robust educational technology.
|Lexile Level
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Train Pontorson → BayeuxFrom HK$210
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realmagick.com The shrine of knowledge.
- There are only 350 of these unbelievably detailed Gouken statues in existence
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Gouken is described in multiple online sources, as addition to our editors' articles, see section below for printable documents, Gouken books and related discussion.
Suggested News Resources
Great care has been taken to prepare the information on this page. Elements of the content come from factual and lexical knowledge databases, realmagick.com library and third-party sources. We appreciate your suggestions and comments on further improvements of the site.
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December 24, 2015, 4:30 pm-6:30 pm
- Veterans Memorial Center
14th & B Streets
This free, festive dinner is open to everyone! We'll serve a full vegan and traditional dinner to all comers. Donations of desserts are welcome. Please join us for our Thirtieth!
Volunteer signups began the Monday after Thanksgiving at the Co-op. The signup binder is located at the Member Information Center, past the checkstands in the store.
Enjoy these thoroughly awesome photos of our Holiday Meal, courtesy of the Davis Enterprise!


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Efrain Staino is an associate in the firm's Intellectual Property Group. Mr. Staino focuses his practice on patent litigation.
He has experience representing both plaintiffs and defendants in complex patent ligation cases in various district courts across the country and the International Trade Commission. His practice has involved a wide range of technologies, including smartphone devices, LED lights, wireless firewall security, Video on Demand cable systems and MEMS technology. Mr. Staino also has an extensive pro bono practice. For example, he recently helped to reunite a Cameroonian gay rights activist with her 10-month-old baby and partner by securing them humanitarian parole and permanent asylum – a story touted in the San Francisco Chronicle.
Mr. Staino earned his M.S. in electrical engineering from Lund University in Sweden. Mr. Staino received his J.D. from Santa Clara University School of Law, which he attended as a Dean's Fellow, a full academic scholarship. While in law school, Mr. Staino served as technical editor for the Santa Clara Law Review, participated in the Jessup International Law Moot Court Competition and served as a judicial extern at the Inter-American Court of Human Rights in San Jose, Costa Rica. Before law school, Mr. Staino worked as a software engineer for seven years in Sweden and the United States, specializing in embedded software development, video storage and broadcasting, and interactive television.
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text:
A gift for friends of mine who celebrate their 15th wedding anniversary. I had a lot of fun doing these all different. They are covered with royal icing.
CakeCentral.com is the world's largest cake community for cake decorating professionals and enthusiasts.
(if this is a private computer)
Yes please, register now!


text:
I bought the theme Highlight and have not altered the zip file in any way. When I try to upload it, it takes a couple of minutes and then I get a message saying "Are you sure you want to do this? Please Try Again." I tried in Explorer, Firefox and Chrome, thinking maybe it was a browser issue, but I get the same thing from all of them.
Any ideas?
Thanks, Mari
Mari, better you upload it thru FTP but make sure, all files are unzipped.
After that, login your wp admin then active the theme.
I had this exact issue, and I uploaded via FTP and made sure all files were unzipped on the village theme, and then it came up with an error in wordpress saying there is no CSS file???? I looked on the web and a few others are having issues with these theme forest themes? Can you help please.
the first zipfile downloaded – needs to be unzipped.
within that you will find the name of the zipfile that matches the theme. that is the file that Wordpress will accept in the install area.
if you figure out something else that worked for you – please take a few extra minutes to share with the next one of us that gets stuck
I am having the same problem after downloading the theme, Square. I wish this was helpful but 1) there is no additional zip file within the zip file and 2) the link posted above goes to the support home page and when I search the same keywords there "Are you sure you want to do this?" I get nothing. Anyone have any updated suggestions? I just bought this theme and already regretting it.
Hi! You need to unzip the file you downloaded and upload the theme inside it. Please see Solving broken theme issues. Thanks!
I get this error whenever I try to use Quick Edit to add or delete a slug or uncheck Allow Comments on a page. I'm using a Themeforest premium theme, too, but it installed OK, once I understood that I wasn't to upload the zip folder that I received (duh!) but only the themename.zip file inside that folder. But why do I get this error message when I try to change things in Quick Edit mode. Neither the slug nor Allow Comments checkbox show in Edit mode, so there's no alternative.
here's what seems to be evident from the previous posts and others online…
1. You paid for a premium theme. 2. Themeforest gives you two (2) ZIP files… 1 of everything and 1 theme-only ZIP 3. The ZIP's are not accepted by WP Dashboard "are you sure you wanna…" 4. You post here to see if anyone can help you 5. They tell you… A) Don't use the 'theme-only' ZIP file. 6. They tell you… Use the ZIP file that is located inside the 'everything' ZIP file. 7. They tell you.. C) You can only FTP that 2nd ZIP file onto your server, not WP install. 8. Except if you FTP that 2nd ZIP file onto your server, something is broken with it. 9. So maybe you should have listened to the WP Dashboard warning in the 1st place.
Is this about it?
I have only now purchased a premium theme and as experiencing the same issue.
It would seem that the WP Dashboard is warning us that something is wrong with the theme.
The solution on other forums is for the theme author to update their theme because there are missing or broken files involved.
That's… like… only my opinion, dude.
That warning rarely means there is an issue with the theme itself.
Since this thread is 2 years old TF have now created a new way to download offering JUST the theme ZIP, OR the FULL download including help, PSD where applicable etc etc.
That "Are you sure" is normally a hosting thing NOT a theme issue and yes using FTP to upload the theme folder UNZIPPED is the most common solution.


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I just noticed a new release from last night, 0x66a. I also can see that my "to do list" has been reset. I have three HR24s and they are all the same. I'm not sure how to force a rebuild of my "to do list" or is it going to auto rebuild.
Anyone else see this problem?
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Guest Message by DevFuse
New HR24-500 Rel 0x66a Problem
Started by utbronco , Feb 12 2013 09:41 AM
2 replies to this topic
Posted 12 February 2013 - 09:41 AM
(1) THR22 TiVo
(1) SWiM-8
(3) WGA600N Dual Band Wireless-N Gaming Adapter
(1) THR22 TiVo
(1) SWiM-8
(3) WGA600N Dual Band Wireless-N Gaming Adapter
Posted 12 February 2013 - 09:48 AM
If your guide was flushed, it will rebuild as the guide data is repopulated.
Posted 12 February 2013 - 11:54 AM
Give it a day or so.
HD DVRs: HR44 + AM21; HR24
Receivers: H25-500 HD Receiver
Mobile Devices: Nomad
Additional equipment configuration details
Sun & moon help site your satellite dish


text:
- The Royal Thai Embassy deepest regrets to announce that His Majesty King Bhumibol Adulyadej of Thailand passed away peacefully on 13 October B.E. 2559 (2016) at 1552 hours, at the age of 89 in the 70th Year of His Majesty's Reign. A book of condolence will be opened for signing at the Embassy from Monday 17 to Friday 21 October 2016 from 1000 to 1600 hours.
- In observance of the Thai National Holidays, the Royal Thai Embassy will be closed on the following day, 6 May 2016.
- Press Releases : Thailand's Statement regarding a long-range rocket launch by the Democratic People's Republic of Korea on February 7, 2016
- Press Releases : Zika Virus Situation in Thailand
- Press Releases : The case of Mr. Thanet Anantawong's arrest
- On 25 January 2016, H.E. Mr. Sarun Charoensuwan, arrived Seoul to assume his position as the Ambassador of the Kingdom of Thailand to the Republic of Korea.
- Announcement from the Immigration Bureau about a new order regarding classes of aliens ineligible for admission to the Kingdom of Thailand
- Royal Thai Embassy's Official Holidays for 2016
Embassy ActivityView All
- On 4 February 2016, H.E. Mr. Sarun Charoensuwan, Ambassador of Thailand to the Republic of Korea paid a courtesy call to H.E. Mr. Lim Sung-nam, 1st Vice Minister of Foreign Affairs
- On 30 January 2016, the Royal Thai Embassy dispatched a Consular Mobile Unit to Gimhae.
- Thailand joins the Global Green Growth Institute (GGGI).
- On 29 January 2016, Rear Admiral Kriengkrai Anantasan, RTN., Thailand Maritime Enforcement Coordinating Center (THAI-MECC) led a delegation from related agencies of Thailand to have a meeting and exchange ideas with H.E.Mr.Sarun Charoensuwan, Ambassador of Thailand to the Republic of Korea.
- On 26 January 2016, H.E. Mr. Sarun Charoensuwan, Ambassador of Thailand to the Republic of Korea, chaired the first Team Thailand Meeting of 2016 at the Royal Thai Embassy.
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Bracing for New Money Fund Rules
When prime funds switch to a floating net asset value this fall, many investors are expected to find other places to stow their cash.
By Rich Miller and Shobhana Chandra, Bloomberg |
October 8, 2012
Companies added an average 121,000 workers a month in Q3, up from 88,000 in Q2.
By Kate Andersen Brower and Holly Rosenkrantz, Bloomberg News |
January 5, 2012
President bypasses Senate with recess appointments to NLRB.
By Hans Nichols, Margaret Talev, Bloomberg |
September 6, 2011
Proposals target middle-income Americans; aim to spur consumer spending.
By Stephanie Armour, Bloomberg |
August 26, 2011
New NLRB rule could bolster efforts to organize workers.
By Heidi Przybyla, Margaret Talev, Bloomberg |
June 28, 2011
As Aug. 2 deadline approaches, leaders may settle for cuts of $1 trillion to $2 trillion.
By Stephanie Armour, Bloomberg |
June 22, 2011
Rule would bring faster hearing deadlines, streamlined procedures.
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Cheating Quotes, Sayings about adultery
If a guy cheats on another girl for you, he's more than likely to cheat on you for somebody else. As they always say, Once a cheater…always a cheater!
A real man ends a relationship before he starts searching for another.
Cheating is the most selfish thing a person can do in a relationship! If your not happy with the person your with then end it. It's that simple!
– Bryan Burden
Don't lie, don't cheat, and don't make promises you know you can't keep.
If you marry a man who cheats on his wife, you'll be married to a man who cheats on his wife.
– Ann Landers
Cheating on a girl is deeper than people realize. It destroys her outlook on love, her future relationships and peace within herself.
The only things you give yourself when you cheat are fear and guilt.
Being drunk will never, ever, be an excuse for cheating.
Forget who hurt you yesterday, but don't forget who loves you tenderly today.
Being labeled a cheater is the worst ever.
Don't cry over someone that won't cry over you.
Most people end up cheating because they're paying more attention to what they're missing, rather than what they have.
If your boyfriend or girlfriend has no texts in their phone's history, chances are you're being cheated on.
I would prefer even to fail with honor than to win by cheating.
– Sophocles
Don't cheat in a relationship. If you're not happy then just leave.


text:
Full CoverageRight now
Article accused Certus Bank of mismanaging moneyUPDATED 8:21 PM EDT Mar 28, 2014
Certusbank lost millions last yearVideo Transcript
YEAR. CERTUS BANK'S NATIONAL HEADQUARTERS WAS THE FIRST TENANT.... IN THE NEW DOWNTOWN GREENVILLE HIGH RISES KNOWN AS "THE ONE PROJECT." NOW... AN ARTICLE PUBLISHED IN "AMERICAN BANKER".... SAYS COMPANY EXECUTIVES HAVE SPENT HUNDREDS OF THOUSANDS... ON LAVISH ITEMS.... WHILE THE BANK LOST TENS OF MILLIONS. WYFF NEWS 4 INVESTIGATES HAS LEARNED... CERTUS BANK REPORTED A NET LOSS OF 64 MILLION DOLLARS LAST YEAR... ACCORDING TO THE F-D-I-C. WE FOUND THE LOSS IN REPORTS. THE ARTICLE IN "AMERICAN BANKER" CLAIMS.... THEY'VE LOST MORE THAN 100 MILLION IN THE LAST TWO YEARS. AND... GREENVILLE COUNTY TAX RECORDS SHOW.... CERTUSBANK OWNS THREE CONDOS IN THE RIVERPLACE BUILDINGS IN DOWNTOWN GREENVILLE. WYFF NEWS 4 INVESTIGATES HAS LEARNED... EACH WAS SOLD FOR APPROXIMATELY 500 THOUSAND DOLLARS. THE ARTICLE CLAIMS THE BANK SPENT 2-POINT-5 MILLION ON HIGH END REAL ESTATE. AND... IT DIDN'T STOP THERE. "AMERICAN BANKER" CLAIMS COMPANY EXECUTIVES SPENT: font -- 131-THOUSAND DOLLARS FOR CAROLINA PANTHERS GAMES... -- 347-THOUSAND FOR PRIVATE PLANE TRIPS... -- AND NEARLY 1 MILLION DOLLARS FOR ART, A THEATER, AND A TOUCH-SCREEN "MEDIA WALL"... IN THE NEW HEADQUARTERS BUILDING. .... "AMERICAN BANKER" CITED PEOPLE WITH DIRECT KNOWLEDGE OF THE BANK'S OPERATIONS AS THEIR SOURCE. ACCORDING TO CERTUS BANK'S CHIEF CUSTOMER OFFICER... THE COMPANY'S CEO'S ARE OUT OF TOWN AND UNAVAILABLE FOR INTERVIEWS... I INTERVIEWED C-E-O WALTER DAVIS IN JANUARY OF 2011.... HE SAID CERTUS BANK WANTS CUSTOMERS TO KNOW... THEIR MONEY IS SAFE. "it's safer than it ever has been. we are a strong financial institution, we plan on staying strong" CERTUS BANK SENT US THIS STATEMENT TODAY: "We take very seriously the issues raised in the article published by the American Banker. The facts will be established and there is a Board process in place to evaluate the claims and to respond in due course. CertusBank remains a strong financial institution, and our talented teammates are focused on providing quality services to our customers in the Upstate and in the many other communities we serve."-- END QUOTE.
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Brandon & Jake - Badpuppy
Models: Jake Lyons and Brandon Wilde
Studio: Badpuppy
Two beautiful, young looking, new porn stars Jake Lyons and Brandon Wilde are tearing it up on screen. Brandon plows Jake's tight ass and they both have a great time! There's bj's, 69-ing, and fucking in all positions, what more could you ask for from two fiery guys!


text:
FORT WAYNE, Ind./ WEST LAFAYETTE, Ind. (Indiana's NewsCenter) - Despite an overwhelming show of support by IPFW's Faculty Senate, Purdue University has named a search committee to replace Chancellor Michael Wartell.
Wartell has held the position since 1994, but will be leaving it in July, when he reaches the mandatory retirement age of 65.
IPFW's Senate had asked Purdue to consider extending Wartell's contract, but the university declined.
The committee will name the new chancellor by the time Wartell leaves.
What are your thoughts CLICK HERE to leave us a "QUESTION OF THE DAY" comment.
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A fun little interaction highlighting Zaarly Storefronts.
You can check out the rest of the page at www.zaarly.com so long as you're logged out and choose any city other than NYC, SF, KC, Seattle or LA.
keyboard shortcuts:
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Show and tell for designers
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realmagick.com The shrine of knowledge.
Eagle Wheels
A selection of articles related to eagle wheels.
Original articles from our library related to the Eagle Wheels. See Table of Contents for further available material (downloadable resources) on Eagle Wheels.
- The Aeonic Perspective of the Enochian Temples
- The initiatory system of the Enochian Temples divides the path of initiation into four major stages, distinguished primarily by the types and sources of the influences they are sensitive to. Briefly, these stages are: 1. Lunar-planetary, focused in Yesod and...
Magick >> Enochian
- Eihwaz
- It's been less than two weeks since I wrote the article for Jera; so it looks like I'm back on track. This Rune is both fascinating and multifaceted! Both the Sheils and Edred Thorsson identify Eihwaz with Yggdrasil, the Norse World Tree or Cosmic Axis. The...
Divination >> Rune Stones
- Popular Oracles and Omens
- I think it would be correct to assume that man has always been anxious to lift a corner of the veil of the future. In this section I've included several popular oracles, omens and superstitions. Some of these might seem a bit ridiculous, but why not consider...
Psychic Abilities >> Divination
- The 'W' Word (Witch), What Does it Mean?
- The other day I sat down and again tried to define what it is that I am doing. We are talking about beginning our church next year, but a church of what? I am the type that is most comfortable with a clear and complete understanding, so I began at the...
Religions >> Paganism & Wicca
- King James Bible: Leviticus, Chapter 11
- Chapter 11 11:1 And the LORD spake unto Moses and to Aaron, saying unto them, 11:2 Speak unto the children of Israel, saying, These are the beasts which ye shall eat among all the beasts that are on the earth. 11:3 Whatsoever parteth the hoof, and is...
Old Testament >> Leviticus
- King James Bible: Deuteronomy, Chapter 14
- Chapter 14 14:1 Ye are the children of the LORD your God: ye shall not cut yourselves, nor make any baldness between your eyes for the dead. 14:2 For thou art an holy people unto the LORD thy God, and the LORD hath chosen thee to be a peculiar people unto...
Old Testament >> Deuteronomy
- Prometheus and the force of fire
- Look at a flame of a fire or just a small candle. See how mesmerizing the ever-changing forms of the fire are? Do you experience the pleasant warmth, romantic feelings or sense of truly being at home, brought about by the flames? From old fire is regarded as...
Deities & Heros >> Greek
Eagle Wheels is described in multiple online sources, as addition to our editors' articles, see section below for printable documents, Eagle Wheels books and related discussion.
Suggested News Resources
- Is this sphere Goodyear's reinvention of the wheel?
- Here's two reasons why Goodyear's concept tire effectively is a reinvention of the wheel, and not just an innovation. First, it looks quite a bit different.
- Veterinarian opens local office on wheels
- Craig Brakeman, DVM, wanted to make that process easier, so he opened a full-service pet hospital on wheels. The Cherry Capital Mobile Pet Hospital features a surgery room, digital X-ray, bloodwork and dental machines. The 24-foot van now is making ...
- 10th Annual Water & Wheels opens Friday at Cape Harbour
- A three-day event featuring vessels, vehicles and more will return to Cape Coral on Friday. The 10th Annual Water & Wheels will be held from noon to 10 p.m.
- Japan spins wheels as wary consumers stash their cash
- TOKYO (AP) — The world's third-largest economy is stuck in neutral, as wary Japanese consumers and corporations opt to hold onto their cash rather than spend it. The latest data, released Tuesday, showed Japan's GDP contracted at a 1.1 percent ...
- Oklahoma House passes insurance mandate for autism coverage
- Legislation that would require health insurers to cover autism treatment for children was overwhelmingly approved by the Oklahoma House on Wednesday. House members passed the measure 76-20 following an emotional debate between the parents of ...
Great care has been taken to prepare the information on this page. Elements of the content come from factual and lexical knowledge databases, realmagick.com library and third-party sources. We appreciate your suggestions and comments on further improvements of the site.
Eagle Wheels Topics
Related searchesinvercargill high schools
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This interdisciplinary conference aims to focus on the exchange of relevant trends and research results as well as the presentation of practical experiences gained while developing and testing...
Education & Learning, Software
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realmagick.com The shrine of knowledge.
A selection of articles related to apnea.
Original articles from our library related to the Apnea. See Table of Contents for further available material (downloadable resources) on Apnea.
- Astral Beings and Wildlife
- The astral dimension contains a wide variety of what could be called non-physical subtle energy life-forms; or as Carlos Castenada so aptly calls them: inorganic beings. Some of these have a positive nature (nice) and some seem have a negative nature (not so...
Parapsychology >> Astral Projection
Apnea is described in multiple online sources, as addition to our editors' articles, see section below for printable documents, Apnea books and related discussion.
Suggested News Resources
- Bariatric surgery to reverse diabetes, high blood pressure and sleep apnea
- In the US, 25 million people in the U.S. currently have type-2 diabetes and that number is projected to rise to 75-million by 2030.
- Obstructive sleep apnea syndrome in children
- A co-author of the recent Childhood Adenotonsillectomy Trial study discusses key developments in the screening, diagnosing, and treatment of kids with obstructive sleep apnea syndrome (OSAS) as well as current AAP clinical practice guidelines for ...
Great care has been taken to prepare the information on this page. Elements of the content come from factual and lexical knowledge databases, realmagick.com library and third-party sources. We appreciate your suggestions and comments on further improvements of the site.
Related searchesstar children
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meaning of dreams about envelope
beadles about
theosophy music
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Mirror, Mirror
If J.K. Rowling's Harry Potter series is still a children's favorite in 2147, perhaps community theaters worldwide will mount imaginative musical adaptations of Goblet of Fire and Half-Blood Prince, but luckily I won't live to see it. Lewis Carroll's Through the Looking Glass, and What Alice Found There has already stood such a test of time, however, captivating generations of kids with the continued adventures of Alice and the nonsensical magic of "Jabberwocky," the poem she can only read with the aid of a mirror. The Company of Rowlett Performers' Wonderland! takes this rich source material on a fun-filled romp complete with doo-wop, bluegrass and hip-hop adaptations of Carroll's words. So use your parental authority, put the kibosh on the Harry Potter worship for a day and take the kids to see some good old-fashioned, opium-inspired children's entertainment. Showtimes are 9:30 a.m. and 1 p.m. Thursday and Friday; 7:30 p.m. Saturday and 2 p.m. Sunday at the Plaza Theatre, 521 W. State St. in Garland. Tickets are $10, $9 for seniors and $8 for students and children. Call 972-977-7710 or visit corptheatre.com.
Feb. 19-20, 9:30 a.m. & 1 p.m.; Sat., Feb. 21, 7:30 p.m.; Sun., Feb. 22, 2 p.m., 2009
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All the King's Men traces the rise and fall of demagogue Willie Stark, a fictional character loosely based on Governor Huey ""Kingfish"" Long of Louisiana. Stark begins his political career as an idealistic man of the people but soon becomes corrupted by success and caught between dreams of service and an insatiable lust for power, culminating in a novel that Sinclair Lewis pronounced, on the book's release in 1946, "one of our few national galleries of character."
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