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Tiny release today. The Google Plus icon has been updated to match Googles new style.
In addition to this, a couple of helper classes were added to the CSS. Apply
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Update: 3.4.1 adds an IE8 fix.

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Posted by ESPN.com's Mike Sando
Gregory Cowles of the New York Times Book Review offers high praise for David Harris' book on former 49ers coach Bill Walsh. "How Bill Walsh Reinvented Football and Created an NFL Dynasty" spans 385 pages.
Kent Somers of the Arizona Republic breaks out his Flash Player to break down the Dolphins-Cardinals game in Week 2.
Also from Somers: a look at apparent attempts to spur trade talk involving Anquan Boldin. Somers offers a big yawn.
Scott Allen of RaisingZona.com predicts a 31-21 Cardinals victory over the Dolphins.
Mike Tulumello of the East Valley Tribune also brushes off the Boldin story line, relegating it to third-item status in his notebook.
Darren Urban of azcardinals.com found a man who has attended each of the 200 exhibition and regular-season home games in Arizona Cardinals history.
Also from Urban: the Cardinals' emphasis on protecting the ball.
And this: Former Cardinals, Lions and Raiders linebacker Rob Fredrickson is the honorary alumni captain for Arizona's home opener. Fredrickson started 119 career games.
Jose Romero of the Seattle Times says the Seahawks' Lofa Tatupu gets fluid drained from his knee to help him play in games.
Clare Farnsworth of the Seattle Post-Intelligencer says only three offensive starters return from Seattle's victory over the 49ers in Week 4 last season. They are Matt Hasselbeck, Walter Jones and Chris Spencer. WOW.
Also from Farnsworth: all hands on deck for Seattle, even though Marcus Trufant and Tatupu have only two healthy ones between them.
And this from Farnsworth: he's picking a 17-9 victory for Seattle. Take that to the training room, not the bank.
Also from Romero: Bobby Engram says the Seahawks will be fine even though injuries are testing their resolve.
Matt Barrows of the Sacramento Bee assesses the 49ers pass rushers who got away, including Julian Peterson.
Also from Barrows: Will Seahawks coach Mike Holmgren wind up with the 49ers at some point in the future?
Bernie Miklasz of the St. Louis Post-Dispatch says the Rams should pipe down about not getting enough respect. He calls out Jonathan Wade for encouraging Marc Bulger to brush off reporters.
Deb Peterson of the St. Louis Post-Dispatch says the Rams will pay tribute to late owner Georgia Frontiere at their home opener.
Jeff Gordon of stltoday.com says Bulger is feeling the heat from all angles.
Steve Korte of the Belleville News-Democrat says the Rams want to get Torry Holt more inovled in the offense. One catch for 9 yards apparently isn't enough.
Bill Coats of the St. Louis Post-Dispatch assesses the impact of Leonard Little's injury. Rams rookie Chris Long will move to left end, a significant adjustment.
Tom FitzGerald of the San Francisco Chronicle says injuries could make the Seahawks vulnerable against the 49ers.
David Fucillo of ninersnation.com breaks down the 49ers-Seahawks matchups with an eye toward injuries. Additional breakdowns here.
Dan Brown of 49ers Hot Read is still waiting for the 49ers' first interception of the season. The team didn't pick off a pass in preseason or the regular-season opener, a span of 147 pass attempts.


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WelcomeSolution for Clean and Smoke Free Kitchen
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Razorback Greenway Bus Stops
One of the best parts about living in Northwest Arkansas is having access to the Razorback Greenway Trail. At Ozark Regional Transit, we want to encourage the community to utilize and explore the trail. ORT currently has 47 bus stops within 500 feet of the the Razorback Greenway. And don't forget, all ORT buses have bike racks for your convenience.See the Breakdown
Ozark Regional Transit Celebrates an Expansion Milestone
June 28, 2016
June 28th 2016, ORT celebrated a major milestone in expansion. ORT has grown from an eight-route system in 2013, to a 14-route system in 2015; an almost two-fold increase in routes and services.Full Article
Kids Ride Free to Free Lunches
Many local schools are providing a summer meal program to ensure our children stay well fed, even when school is out. There is no need to worry about transportation to the locations, ORT services almost every school on the list. And don't forget, KIDS RIDE FREE all summer long!More Info
Square 2 Square Bike Ride
Saturday, May 14, 2016, 8:00 AM - 3:00
Ozark Regional Transit is excited to be participating in the 2016 Square 2 Square Bike Ride. The Square 2 Square event starts at the Fayetteville Square and follows the Razorback Greenway for 32 miles to the Bentonville SquareRead More
Northwest Arkansas Quality of Life Survey
We all know that Northwest Arkansas is a great place to live, but a recent study confirms it. 95% of Northwest Arkansans report being very happy and believe they have a high quality of life.Read More


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Missed the live Ask the Expert event?
Not to worry. Check out the archived thread of the Q&A with Ken Hutcheson, President of U.S. Lawns, and the LawnSite community in the Franchising forum .
Echo weed whip get too hot
Discussion in 'Mechanic and Repair' started by the Lawnmower Man, Jul 14, 2008.
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You know how every once in a while you read a blog you love and stumble upon an idea so unique and fun you can't help but smile? Such is the case with You Are My Fave (by Babble contributor Melanie Blodgett) and her In A Box series. The concept of In A Box is simple, featuring beautifully styled and colorful collections that serve as the ultimate party in a box or gift in a box! Most are easy to recreate and would be a really awesome way of celebrating a special occasion or a special person. Want to see more of what I'm talking about? Click through for 10 awesome party in a box ideas![collection type='slideshow' style='classic']
Article Posted 3 years Ago


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– Stephen Hunt is managing director of Rockingham Retirement. The opinions expressed are his own. –
The Great Debate UK
from UK News:
Is a 1.8 percent inflation rate good or bad news?
- Sumeet Desai, Reuters senior UK economics correspondent. -
Inflation unexpectedly held steady in July, official data showed Tuesday, but economists still expect big falls in the annual rate this year and monetary policy to stay loose for some time to come.
Enjoy low inflation while it lasts
– David Kuo is director at the Motley Fool. The opinions expressed are his own.-
Predicting the economic effects of swine flu
– Marie Diron is senior economist at Oxford Economics. The opinions expressed are her own –
from The Great Debate:
Beware reflation
from The Great Debate:
China risks overcooking the economy
-- Wei Gu is a Reuters columnist. The opinions expressed are her own --
While China has been outspoken in expressing concern about the United States printing too much money, those worries might be better focused at home. No country beats China when it comes to effective monetary easing.
from The Great Debate:
Europe frets over crisis exit strategy
-- Paul Taylor is a Reuters columnist. The opinions expressed are his own --
Higher taxes? Lower public spending? Devaluation? Inflation? Investment in green growth?
from The Great Debate:
First exit for the Fed
-- Agnes T. Crane is a Reuters columnist. The views expressed are her own --
Call it a battle for beginnings and endings, and the Federal Reserve is smack in the middle.
Pensioners feel pinch from low rates
– Sharon Bratley is chartered financial planner at Fair Investment. The opinions expressed are her own. –
Deflation? It's inflation you need to watch
— David Kuo is a director at the financial Web site The Motley Fool. The views expressed are his own. —


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What Rare Communist Summit Means for N. Korea's Future
What Rare Communist Summit Means for N. Korea's Future
11 results for 'communist china'. Showing results 1 - 11
What Rare Communist Summit Means for N. Korea's Future
Scores of people are dancing, blowing shofars, and worshipping God. You might think these images come from a charismatic...
Iran denies it is hiding evidence of nuclear weapons activity even after satellite photos show work being done at...
China Christians Form Human Shield to Protect Church
After the Supreme Court's recent gay marriage rulings, the media reported that America had a rapid change of heart...
Obama Files Trade Complaint Against China
Although China is officially an atheist state, in recent years it has allowed people to celebrate this very significant...
Homosexual Agenda Pushed in Movies Like Brokeback Mountain
A Beijing restaurant run by Christians serves up salvation along with meals.
Carter in N. Korea in Bid to Free U.S. Christian
Communist China Marks 60th Year


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Greater Transparency and Accountability Key to Improving Effectiveness of Israel Police
A RAND study addressed issues of public perceptions and trust in the Israel Police, and benchmarked Israel's law enforcement agencies against other police organizations. This year, recommendations from the report have been adopted by the Israel Police, including the launch of a pilot program for the use of body worn cameras. NSRD director and RAND Vice President Jack Riley, and Senior Economist Steven Popper are among the study's lead authors.


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South Florida's 25 Best Dishes
One of the things that best defines the disjointed urban sprawl of Broward and Palm Beach counties is food. Food comforts and satiates — it makes our daily commute down I-95 bearable, and it helps us look past our differences and come together.
As a run-up to our Best Of Broward-Palm Beach edition, which comes out next week, we began in January compiling a list of our 100 most indispensable dishes. You can find the full list on our food blog, Clean Plate Charlie. Here, we've distilled that list down to our 25 best dishes. These are our most unique, praiseworthy, and essential plates; meals that truly define what it is to eat in South Florida.
In this tasty time capsule, we've placed smoky barbecue that has been made the same way for more than 50 years and meatballs that have won praise the world over. We've got taco joints that stay open late to feed the beer-swilling proletariat, donuts made with crispy bacon, and seafood plucked fresh from our coastal waters. Most of all, we've got flavor.
So read up, and eat up. This is what South Florida tastes like.
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Florida Panthers v New Jersey Devils
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No. 25: Chicken Wings from Anthony's Coal Fired Pizza
No. 24: French Toast from Dune Deck Cafe
No. 23: Fried Chicken from Fran's Chicken Haven
No. 22: Milk Shake from Jaxson's Ice Cream Parlor
No. 21: Key West Dolphin Sandwich from the Whale's Rib No. 20: Nobu Snapper from Sushi Simon
No. 19: Pink Vanilla Cupcakes from House of Sweets
No. 18: Cheese Steak from Sonny's Famous Steak Hogies
No. 17: Chicken from La Granja
No. 16: Italian Special Hoagie from LaSpada's
No. 15: Goat Cheese Cheesecake from Satoro
No. 14: Falafel from Sunrise Pita
No. 13: Chipotle Pork "Florito" from East Coast Burrito Factory
No. 12: Crawfish Boil from Rosey Baby Crawfish & Cajun House
No. 11: Chicago Dog from Hot Dog Heaven
No. 10: Salsa from Zona Fresca
No. 9: Chicharron Tacos from Tacos al Carbon
No. 8: Foie Gras from Michelle Bernstein's at the Omphoy
No. 7: Burger from Jack's Old Fashion Hamburger House
No. 6: Meatballs from Cafe Martorano
No. 5: Chopped Beef Brisket from Tom Jenkin's Bar-B-Q
No. 4: Wood-Fired Pizza from Sicilian Oven
No. 3: Maple-Bacon Donut from the Office
No 2: Chopped Pork Sandwich from Georgia Pig
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Peter R Orszag, New York, US, Warren St, Apt 898
View Peter's social profiles and photos on Facebook, MySpace, and +40 Networks.
Wikipedia: Peter Orszag
Peter Richard Orszag (born December 16, 1968) is an American economist who is a Vice Chairman of Global Banking at Citigroup. He is also a columnist at Bloomberg View, a Distinguished Scholar at New York University School of Law,
BlinkX Video: White House Budget Director Peter Orszag: The Man Behind the Budget Plan
Information about the Man who is behind the Budget Plan for our country. ... White House Republican Democrat Peter Orszag Obama Budget New York Times Health Care Reform Diet Coke... - YouTube
Tilting Power to Executive Branch
28.07.2010 [New York Times (blog)] - By THE NEW YORK TIMES In his latest Political Times column, The Times's Matt Bai considers how recent efforts from Peter Orszag, the outgoing budget
Four Ways to Kill a Climate Bill
26.07.2010 [New York Times] - Peter Orszag, the budget director, said giving away pollution permits would be "the largest corporate welfare program that has ever been enacted in the
A legacy of budget trickery
25.07.2010 [New York Post] - No more budget gimmicks? That's what outgoing White House budget director Peter Orszag promised as the Obama team prepared to take control
The Early Word: Setting Priorities
14.07.2010 [New York Times (blog)] - You've Seen His Math Before: Mr. Obama nominated Jacob Lew to replace his outgoing budget director, Peter Orszag. Mr. Lew, The Times's Peter Baker recalls
Twitter Posts: New York Times Wire (nytimes_wire)
2010.07.28 Budget Chief Tilted to Executive Branch: Peter Orszag, who steps down Friday, tried to wrest budgeting responsibi... http://nyti.ms/9pI1A2
Reason Writers Around Town: Peter Suderman in The New York Post on …
26.07.2010 - — Authority 705 Budget director Peter Orszag'slegacy of budget gimmickery. No more budget gimmicks? That's what outgoing White House […] budgetdirector Peter Orszag promised as the Obama team prepared to takecontrol of the White House. "The president prefers to tell
NY Times Columnist Sent Obamacare Article Draft To Valerie Jarrett For …
19.09.2012 - — Authority 663 (WEEKLY STANDARD) The latest Bob Woodward books reveals that Peter Orszag, at the time a columnist for the New York […] Methodology." The bottom of the article carries this biographical information for Orszag: "Peter Orszag, the director of the
Lap dog media bias
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Peter Orszag to Join Citi as Vice Chairman in Global Banking
Citi Strengthens its Banking Franchise with Key Hire. New York – Citi announced today that Peter Orszag has joined as a Vice Chairman in its Global Banking business.
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Wednesday, March 16, 2011
Japan tragedy affects Alaska seafood industry
Here's a story by Hal Bernton of The Seattle Times on the impact of Japan's earthquake and tsunami on the seafood industry.
Labels: earthquake, Japan, tsunami
1 comment:
- Seafood Supplier said...
I am agree that Japan tragedy has really effect the seafood industry. Now few places are left safe where we can caught seafood.
- April 13, 2011 at 11:42 PM
Subscribe to: Post Comments (Atom)


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If you are new to social media marketing world or not sure you're doing the right things, this infographic is for you. It illustrates 12 simple tips to social media success.Here are a few of them:Research buy 1000 arabic twitter followers and know your audience! What topics and interests are they most social about?Only choose to utilize the networks that best speak to your audience.Write a social media marketing playbook. The playbook should detail your key performance indicators, audience profiles, brand personas campaign concepts, promotional events, contests, content themes, crisis management steps, etc. Note that the strategy should be unique to each platform.Take 30-60 minutes at the beginning of each week or month to schedule tweets, Facebook posts, LinkedIn posts, pins, or other social media content. These can be original ideas, links to your own work, or links to outside content that may be useful or of interest to your audience.
If you think about it, social media is like an online version of word-of-mouth advertising. One user on a social site may favorite, follow, or Digg something about you, and suddenly your brand and name is now being shown to all of their friends, and if they repost the mention, they open your brand up to all of their friends, and so on down buy arabic twitter followers the line.Businesses can start with a company presence or profile on different, but appropriate, social media sites. You can create a Fanpage on Facebook, get your employees on LinkedIn, sign up for Twitter, or start submitting relevant articles and content to social bookmarking sites, like Digg and StumbleUpon. You can also use free blogs like Blogger to open a dialogue between your business and consumers. When creating profiles on these sites, remember to link them up. For example, on your Twitter profile you can include a link to your blog or Web site. On Facebook you can install a Digg application so others can see what you favorite, and a Facebook Twitter application lets your Tweets become your Facebook status update. Twitter feeds can be added to your blog, and so on. By linking up your profiles, with one update you can blast your message to multiple social sites.
I think the reason Melissa Coker's video for Wren became such a viral sensation is because it was a completely original concept, a bit risky, and it resonated emotionally with all audiences regardless of who the person was. Everyone buy 500 arabic twitter followers has (or wants to!) kiss a stranger at some point in their lives, and watching funny, quirky, intimate moments between people in that video hit a nerve with audiences, while simultaneously exposing them to Wren in the most subtle way imaginable. Think about what you instinctively want to see -- is it something scripted and salesy? Or would you be more interested in universal topics like love, relationships, unguarded moments, friendships, intimacy, confessionals, and the like? Equinox just released a short, super simple video of a woman doing morning yoga in her home and it has over 7.5 million views in just a couple of weeks. There's nothing sensational or spectacular about it, but it's very beautiful and intimately shot, and shows a physical grace and fitness that most everyone strives for. Content that is authentic and touches upon a common human concern tends to be very powerful.
641 N. High Street
Columbus, Ohio 43215
(614) 227-0151
Hours and Location


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realmagick.com The shrine of knowledge.
Aloe: Correspondences
Aloe Vera
Folk Names: Burn Plant, Medicine Plant
Gender: Feminine
Planet: Moon
Element: Water
Powers: Protection, Luck
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© 2000 - 2016 Realmagick.com, All Rights Reserved.


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'12 Years a Slave' screenwriter John Ridley on where he'll keep his Oscar
In the video interview from Oscar night below, Mequon native John Ridley, who the adapted screenplay award for "12 Years a Slave," tells Vanity Fair where he'll keep it.
Spoiler alert: He's sending it to his parents.
"They deserve it. I wouldn't be here without them. That's where it deserves to be." »Read Full Blog Post
Mequon native Ridley wins screenplay Oscar for '12 Years a Slave'
Mequon native John Ridley won the adapted screenplay Oscar for "12 Years a Slave," in ceremonies Sunday.
The wrenching film is based on the memoir of Solomon Northup, a free black man who was kidnapped and sold into slavery in the pre-Civil War South.
"All priaise goes to Solomon Northup," Ridley said in a brief acceptance speech. "Those are his words, that is his life." He also thanked his parents, who still live in the Milwaukee area. »Read Full Blog Post


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Release Date: May 12th, 1992
PG|1 hr. 40 min.
Plot Summary
Architect Newton Davis (Steve Martin) is distraught when his girlfriend, Becky (Dana Delany), spurns his proposal and refuses to move into the lavish house he designed for her. Davis, who cannot imagine himself living alone in the house, shares his tale of woe with Gwen (Goldie Hawn), a dishonest waitress he thinks doesn't speak English. Complications arise when Davis, on the brink of reconciliation with Becky, discovers that Gwen has moved into the couple's soon-to-be dream home.
Cast: Steve Martin, Goldie Hawn, Dana Delany, Julie Harris, Donald Moffat, Peter MacNichol, Richard B. Shull, Laurel Cronin
Director: Frank Oz
Genres: Comedy


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Location. Ritz-Carlton Reserve Phulay Bay is located in Krabi, close to Tub Kaek Beach. Additional area points of interest include Ao Nang Krabi Boxing Stadium and Nopparat Thara Beach. Hotel Features. Dining options at Ritz-Carlton Reserve Phulay Bay include 5 restaurants. A poolside bar and a bar/lounge are open for drinks. Room service is available 24 hours a day. The hotel serves a complimentary breakfast. Spa amenities include massage/treatment rooms and a hair salon. This 5-star property has a 24-hour business center and offers a meeting/conference room, technology support staff, and audio-visual equipment. Complimentary wireless and wired high-speed Internet access is available in public areas. This Krabi property has 100 square meters of event space consisting of a conference center and banquet facilities. Business services, wedding services, concierge services, and tour/ticket assistance are available. Valet parking is complimentary. Additional property amenities include a rooftop terrace, a library, and a terrace. Guestrooms. 54 air-conditioned guestrooms at Ritz-Carlton Reserve Phulay Bay feature hair dryers and irons/ironing boards. Wired high-speed and wireless Internet access is complimentary. Televisions have pay movies. Outdoor infinity swimming pool featuring comfortable oversized day beds and cabana. Gym is over 200sqm in size, with state of the art techno gym fitness equipment. Locker rooms and shower facilities are available. Chandara, our Club House & Library features cozy and comfortable library with international books in numerous audio and visual materials. The Club Selections of board games, and entertainment programs tailor made as the guest requests. Additionally Club House offers computers with internet connection and printing facilities.
Get Directions
|111 Moo 3, Nongtalay, Muang, Krabi 81000, Thailand (Get Directions)
|Click to show number


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Not Accepting Guests
- Last login about 1 month ago
Join Couchsurfing to see Karabo's full profile. It's free!
About Me
Always excited to make new friends and networks all over the world.
Pull through to South Africa and you can fill in the rest .....
Why I'm on Couchsurfing
The best things in life are free ... "dadada dum"
Exploring other cultures, sport and social interactions of any sorts
- wildlife
- books
- architecture
- traveling
- sports
Music, Movies, and Books
Scar tissue,
Long walk to freedom
Steve Jobs
Red hot chilli pepers
Deep Tech
any crazy tunes
One Amazing Thing I've Done
I did an exchange programme in germany a couple of years back and i was able to do a eurotrip with some school freinds in cities and countries like paris, amsterdam, berlin and travel through belgium and luxonburg.
Teach, Learn, Share
Come to South Africa and dont just read books about how bad the crime is our wildlife, architecture and people are super, and we do acknowledge we have some problems which we are trying to fix like every developing country. Our climate is amazing although i wish it snowed once in a while so i can expierence the feeling.
What I Can Share with Hosts
Laughter 4sho
Countries I've Visited
Belgium, France, Germany, Netherlands, Norway, Sweden, Thailand
Countries I've Lived In
Germany, Sweden


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Tomasz Szulczewski
My name is Tomasz Szulczewski but my English-speaking friends call me Tom. I was born in Poland and I still live there, although most of the time I'm away. I'm a professional seafarer, meaning that I spend pretty much half of my life at sea. It does have some obvious (and not so obvious) pros and cons…
I picked up my first camera over a quarter of century ago, at the age of 10 (or thereabout). It was a Russian replica of Leica – Zorki 4 and I got it from my dad, who showed me how to take photos and then how to develop them. Those were the times of BW photography and… lack of good equipment and supplies. I've never signed up for any workshops or took any lessons. It was all a series of hit-and-miss experiments, both in the field and in the bathroom that was serving as a makeshift darkroom (much to my mother's dismay). My biggest dream at the time was to have a lightmeter…
Now, fast forward to early 90's: World has changed and 1hr photo lab services are around every corner. Colour photography is having its prime time and I'm finding myself looking more and more towards SLR field. Few years later I'm faced with a challenging task of learning this advanced photo editing software: Photoshop 3.51 (without CS but with layers!). Like with every diligent student, where's need there's… coffee. It was then, that I've learned about sharpening, raster angles, masking and many more. In the photography field, the end of the century was time for shooting Velvia and dreaming of affordable drum scanner…
It was not until the year 2001 that digital cameras were finally able to produce acceptable pictures. My first digital camera was Fujifilm FinePix 2400z. No more film costs and instant results. Brave New World of digital photography was opening it's gates… but I wanted more. Like with any point and shoot camera, with the small Fuji-thingy creativity was somewhat limited. Being a control freak like myself, I knew I needed digital SLR. In 2002 Nikon introduced D100 – the first DSLR that didn't require selling a kidney on black market (although still rather expensive toy at the time). I bought it in October same year and embarked on a journey that still continues…
It's difficult to describe or categorise my work. I'm not one of those "accomplished" photographers that have their own unique style. Landscape and travel photography is probably a term that will encompass the biggest part of my photographic art, but truth is that I shoot whatever catches my eye. My favourite subjects will always be people and nature and preferably the combination of both. I'm self taught, without any "official" artistic/photographic background. My work has never been widely published in any magazines nor had it won any contests. I guess it would help if I actually submitted some of it in the first place and I think I'll finally pull myself together to do it. I simply never felt the need to do it. Being a professional photographer was always my dream, but as always, reality check was telling me otherwise.
It was like this until the day I decided to check what's the whole fuss about Google+. At first I've signed up and… left it alone. I didn't quite know what to do with it. Then I found "The Guide to Photographer's Paradise", a pdf guide written by Colby Brown and decided to give it another try. It was a rollercoaster ride ever since and I've been spending more time and energy on my photography than ever before. The results you can see on gallery pages of this site or in my streams on G+ and Facebook
1,121 Affection
29,907 Photo Views
73 Followers
189 Following
Mielno, Zachodniopomorskie, Poland


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Siemens Building Technologies, HVAC Products Division: VARIABLE-FREQUENCY DRIVE
August 31, 2002
The SED2 (Siemens Easy Drive) variable-frequency drive has the company's SBT P1 protocol and Johnson Controls' NCI N2 (Metasys®) communications built in. This drive also offers direct connection with other networking protocols, such as Lon and Modbus devices, through optional interface modules. The company states that the drive seamlessly integrates with the APOGEE brand of controllers. According to the manufacturer, features include extremely low harmonics without the use of external devices, pump controller with cascading functionality, belt failure detection, multilevel programming, essential services mode (smoke purge), and digital and analog I/O, including direct Ni 1000 RTD sensor level inputs.
Siemens Building Technologies, HVAC Products Division, 1000 Deerfield Parkway, Buffalo Grove, IL 60089; 847-215-1000; 847-215-1093 (fax); www.sbt.siemens.com (website).
eProduct #190
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Prices, specials, features and availability subject to change.
Contact for details
Trinity Village at Farmer's Creek Apartments in Dallas, TX is exactly where you want to be. It's just a few minutes from parks, festivals and delicious Restaurant Row eateries in Addison, TX. Plus, our proximity to the Dallas North Tollway makes commuting a breeze. We offers some of the best apartments for rent in the Plano School District, come live close to the top-rated North Dallas schools. Make our Far North Dallas Apartments your next home.
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Dogs Allowed. Cats Allowed. Pets Accepted. Call for service animal policy.
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Hepatitis in viagra in bph patients with surgical complications of pulmonary tuberculosis. Ansari MM, Beg MH, Haleem S. 19 Hepatic Injury from Antiviral Agents MAURIZIO BONACINI California Pacific Medical Center, San Francisco, California, U.S.A. 60.
Indian J Chest Dis Allied Sci 1990.
Β-THALASSEMIAS Two main variants viagra in bph of β-thalassemias exist. In the β0-type, the levels of HbF are raised, HbA is absent, and HbA4 has a variable concentration. In the β0-form, no β-chains are produced, and in the β+-form, a reduced amount of β-chains is produced. The molecular pathogenesis of β-thalassemias is heterogeneous.
The α-thalassemias are more frequent in persons of African, Mediterranean, or Asian descent, whereas the β-thalassemias are more frequent in persons from Southeast Asia. More than 240 different mutations in the β-globin gene have been described. And some involve small deletions, most lesions are point mutations. 5.2.1.
Su GL, Klein RD, Aminlari A, Zhang HY, Steinstraesser L, viagra in bph Alarcon WH, Remick DG, Wang SC. Kupffer cell activation by lipopolysaccharide in rats. Role for lipopolysaccharide binding protein and toll-like receptor 6.
Specifically, neuropathological disorders affecting frontal systems may viagra in bph impair a person's self-regulatory processes and self-awareness, which in turn may hinder cognitive processes that are core components of the competency to consent to treatment or research. T. Palmer Jason H. Yet there is legitimate concern about the competence of persons with neuropathological disorders affecting frontal systems to provide informed consent to enter clinical research.
In particular the competency to refuse a prescribed treatment, similar concerns occur in treatment settings. Karlawish Clinical research is necessary for the advancement of science and to improve clinical methods.
C. (1992). A., and Kessler, R. C., Warner, L. Comparative epidemiology of dependence on tobacco, alcohol, controlled substances, and inhalants.


text: The Fancy Bears hacking of the WADA/Olympic data base unearthed Bradley Wiggins' TUE use just before three important events has caused rumblings of doubt and calls of stretching the letter of the law. Wiggins has not broken the rules as they stand, but there is the moral question and whether or not the TUE system is wrong. Joe Harris and Steve Maxwell of The Outer Line ask; is it 'Treating or Cheating?'


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Fusion Question
Can you fuse a Pearl Pistol to a Weber Rail Gun? What i mean is you get the Glowing look of the Weber Rail Gun and the stat of the Pearl Pistol... This is to get the Pirate Bonus for the Pirate set... Thanks in advance guys!
Boss Uniques can't be fused. As stat items nor image.
Alright.. so that only means i cant get the Pirate Set with a Glowing Weapon.. *weeps* :((
Anyways thanks Teto!
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- {{matchup.date.fullday}}{{matchup.date.month + ' ' + matchup.date.day}}
Boston College Defeats Maryland 69-58 - Recap
Boston College got 26 points from Olivier Hanlan en route to a 69-58 victory over Maryland on Tuesday at Conte Forum.
Boston College won and also put money in the pockets of their supporters by covering the 1-point spread as underdogs.
Boston College got 13 points from Lonnie Jackson and another 8 points from Patrick Heckmann. Patrick Heckmann contributed 2 assists, while Eddie Odio cleaned the glass with 11 rebounds.
Maryland got a 26-point performance from Logan Aronhalt, who was supported by 9 points from Dez Wells. Nick Faust had 5 assists in a losing cause for Maryland, while Alex Len grabbed 8 rebounds.
Boston College trailed at halftime, 33-26.
Boston College won the battle of the boards, outrebounding their counterparts 37-32.


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Maybe Accepting Guests
- 0% response rate
- Last login 12 months ago
Join Couchsurfing to see Eternal's full profile. It's free!
About Me
LoVe - LiViNg iT & GiViNg iT...
Sometimes wonder If I was South American in a former life as I am a sucker for all their music, and the rhythm makes me sooo happy! :) But my philosophies are largely Eastern, with a splattering of Native American teachings.
A dreamer and a doer! Am always willing to leap into unexplored territory, just to dip my toes into the beautiful mystery and magic of what's out there. Everywhere, there is something incredible waiting to be known.
I adore most animals, wild life and occasionally dressing up as a panda with wings. I've been a vegetarian for over 12 years now. Though my mouth still waters (close your eyes veggie folk) when I smell a sizzling tender steak, after living next to a cow abattoir during university, I heard their echoing screams nightly. I went to take a peek at what was causing such a screech. Have you ever looked a cow eye to eye and watched helpless as they cry with misery? It made me rethink on the spot my pleasure of eating these cute eyed fellows or infact any other creature.
I totally believe it is possible to make a difference if we each do a little bit, have witnessed the reverberating effects of small doings creating sublime changes.
Happy to lie on the grass gazing at hazy clouds or take part in a tribal dance, wearing mock leopard skin and ear muffs.
Feeling blessed at life's gift and though the journey is bumpy, learning to love this ride!
There's an awesome higher power, a universal energy BUT
We are still masters of our fate.
We are still captains of our souls.
~Winston Churchill~
Anything's possible, just try!
You need chaos in your soul
to give birth to a dancing star
~Frederick Nietzsche~
Why I'm on Couchsurfing
CS inspired me to write again and I started 'Safari Minds' - a CS writing club, with the mission of pushing creativity out on every level possible. Those 'Safari Minds' mean the world to me and are still my best friends today, thanks to Couch Surfing giving me the opportunity to befriend such unique and talented beings!
What's better than to connect, share ideas, and joy. Groove on and thy shall receive! ;0D
Its been one awesome magical carpet ride! This international community is seriously fabulous! I've been forever altered. In a good way, mind you.
* Energy * Growth * Creativity * Music * Writing * Understanding * Yoga * Children's laughter * Moonlight * Vision * Hiking * Blazing Stars * Spring * Faithful Friends * Nature * Learning * Exiting Comfort Zones * Passion * Compassion * Philosophy * Taking Semi-Calculated Risks * Pranic Healing * Humility * Bongos * Marzipan * Sustainability * Sunsets * Holistic Living * Rainbows * Exploring my unknowns and what I thought I knew * Tablas * Values * Shamanism * Buddhism * Hinduism * Sufism * Aruveyda * Social Justice * Cooking up a treat * Harmless Mischief * Log fires * Saxaphone * Astrology * Spirituality * Sneezing * Dancing * BBQs * Poetry * Community * Laughing * Adventure * Permaculture * Photography * Curiosity * Travel * Dreaming with my eyes wide open * Meaningful Conversation * Science * LOVE * Being * Pomegranates * Purpose * Gardening * The Road Less Travelled * Self-Leadership * Veganism * Recycling
- animals
- writing
- poetry
- photography
- dancing
- cooking
- vegetarian
- vegan
- yoga
- clubbing
- gardening
- traveling
- billiards
- magic
- music
- hiking
- surfing
- buddhist
- teaching
- science
Music, Movies, and Books
Mind candy:
Autobiography of a Yogi, Outliers, Episodes of the Cuban Revolutionary War 1956-58,Paradise Lost, Outliers, Eleven Minutes, Veronika Decides to Die, Mutant Message Down Under, Shantaram, Einstein His Life and Universe, A Short History of Nearly Everything, Jonathan Livingstone Seagull, Lao Tzu,Cook books (well the pictures anyway) ;). Osho, Nietzsche, Tagore, Krishnamurti, Sufi & Rumi texts.
Any cinema that makes my mind wonder, heart soar, eyes revel, smile muscle ache or soul flutter: Most David Lynch, Lars von Trier & Tarkovsky stuff. Green Mile, Ray, Instinct, Dances with Wolves, Your mother's home videos, The Gladiator, Amelie Three Colours, Lord of the Rings, Rain Man, Dogville, Narnia -[The Original Series though] (was one of those daft kids that used to sleep in my cupboard, wondering when it would open up), Forest Gump, Black, Meet The Fockers, Crouching Tiger Hidden Dragon, Mary Poppins, Babe, Back to the Future, The Color Purple, Superman - The original (Soo wanted to be super woman!), American Beauty, Sabrina, Le Parfum, Zoolander, The Witches, Willy Wonka & the Chocolate Factory -The Original (NOT the Johnny Depp version), Elephant Man, Drive, Stalker, Dancer In The Dark, Tree of Life, La Môme, Manon Des Sources, Drive.
Dreamin' ears:
The XX, Paco De Lucia, Ladysmith Black Mambazo, Bob Dylan, Buddha Bar, Nitin Sawhney, Bob Marley, The Doors, Bocelli, Elvis, Zeplin, Rolling Stones, Fleetwood Mac, Amadou & Mariam, Belly Dancing Beats, Spanish Guitar, Deep Forest, Most things Cuban, Bangra Beats, Pink Floyd, Leonard Cohen, Disco, Sergio Mendes, Salif Keita, Paolo Nutini, Pavarotti, Celloman, Arabian Chill, South American Wood Wind, Tracy Chapman, Michael Jackson, Gotye, Africando All Stars, Kizomba, Van Morrison, Nina Simone, Ravi Shankar, Florence & The Machine, Adele, Damien Rice, Bigott, Creedence Clearwater Revival, Beirut, Bon Iver, Otis Redding, Little Dragon.
One Amazing Thing I've Done
Homeless, orphaned, tsunami children giving to me with utter selflessness, when everything of value, on every level was stripped from them... humbling and priceless.
Teach, Learn, Share
Every person who crosses my path today is my teacher.
Human beings have this incredible ability to teach one another to evolve, knowingly or unknowingly and tap into a pool of higher potential.
CS is about taking that journey.
Its easy to love those close to you, but learning to genuinely give love to all beings and life is a blossoming journey.
I can teach you how to cook up unique vegetarian and vegan dishes, am passionate about quality veg cuisine! Can also teach you about self-leadership and healing yourself as currently facilitate around these subjects.
Countries I've Visited
Belize, Canada, China, Cuba, Germany, Greece, Guatemala, Hungary, India, Indonesia, Italy, Malaysia, Mexico, Monaco, Morocco, Portugal, Singapore, Spain, Sri Lanka, Sweden, Thailand, Tunisia, United States
Countries I've Lived In
France, Japan, Malaysia, United Kingdom


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Football: Beallsville Bows Out
Ohio Division IV, Region 25
Glouster Trimble 61, Beallsville 0
GLOUSTER, Ohio – No. 1-seeded and undefeated Glouster Trimble (11-0) racked up 386 yards of total offense and intercepted a pair of Beallsville (6-4) quarterbacks three times each in advancing to the regional semifinals.
Mikey Palmer paced the eighth-seeded Blue Devils with five catches and 66 yards.


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Wise heads have wondered about ways to convert cred into food and shelter. So far, the alchemy has proven inconclusive, but if they ever get the formula down, this T-shirt is worth at least a six-pack and a single night's lodging in the back of a tour van. Hardcore pioneers Black Flag ended their career playing jazzed out sludge metal jams, a hybrid that excites only the most completist of record store clerks (a shrinking demographic itself). Black Flag guitarist Greg Ginn is a documented Dead fan and as far as I know this is the only shirt out there cutting Sugar Magnolia lyrics with iconography from both bands. Get it from Jeremy Dean, the man behind The Hardcore Archaeologist, and someday trade it for beer.
Pete Anderson is a writer living in Washington D.C. Read his blog here and follow him on Twitter here.


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Kullandığınız Browser desteklenmemektedir
Lenovo support web sitesi Microsoft© Internet Explorer 10+, Mozilla Firefox 29+, Chrome 34+ Browserlarını desteklemektedir
The default installation of the required prerequisites for Intel WiDi functionality does not enable WiDi.
The installation of the Intel WiDi driver fails with the following error message below.
|Affected configurations
The above symptom may occur on the following systems:
System is Configured with:
Applies To:
This issue is under investigation by Intel.
Install the My WiFi component of the Intel Wireless driver. Select the Custom install instead of the Typical install. Ensure that the Intel My WiFi Technology is selected as shown below.


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Top South Africa holiday reviews
South Africa is home to a cultural tapestry like no other, with indigenous tribes such as the Xhosa and Zulu side-by-side Afrikanas and people of other European descent, as well as many Indian and Jewish communities. This diverse cultural backdrop has resulted in unique traditions, customs and cuisines, and makes South Africa an extraordinary country to visit. Blessed with the magnificent Stellenbosch wine region, the epic Kruger National Park, Table Mountain and surrounds, it's now surprise that South Africa is fast-becoming a leading destination for travellers wanting to experience the diversity of Africa.
Top reviews on South Africa holidays
If you want to find out more about a holiday in South Africa but don't want to take our word for how incredible a destination it is, why not browse through our South Africa holiday reviews below? Written by real Intrepid travellers, they've experienced the country first hand on one of our trips, and their feedback can help you when researching your own South Africa holiday.
Vic Falls to Kruger , October 2016
Luca Valori
My trip with Intrepid Travel was just one of the most exciting traveling experiences of my life. A safari from Victoria falls to Kruger National park. Our team leaders Robert and Schedric have just been amazing. All the places we've visited were outstanding. I loved to play an active role (helping preparing meals, cleaning and setting the camps). I met wonderful people from all over the world. I'll definitely travel with intrepid again! See you all soon!!
Review submitted 21 Oct 2016
Kruger to Vic Falls , September 2016
David Reed
Thoroughly enjoyed the trip. Intrepid team and group of 10 travelers all got on very well. Meals and camping facilities were great. Knew beforehand it would involve many km of travel but Palapye and Nata days could have improved local activities. Would recommend for first time travelers to Africa who want to see a little of three countries and experience the two big sights of Kruger and Vic Falls. Tips- Bring: earplugs + tent light&torch for camping, long lens camera, alarm clock, inflatable pillow, sense of adventure
Review submitted 18 Oct 2016
Kruger & Coast , September 2016
charles jones
This is a bus trip primarily between lodging in campsites and/or hostels. Sometimes those lodgings are in the town named in the itinerary and other times your lodging is so far from the named town that it is hopeless to spend any time in the town to gain local flavor.
Review submitted 18 Oct 2016
Kruger Experience - Lodge (5 days) , October 2016
Tracey Ashton
This was a great introductory trip to South African safari. It was exceptional value for money and would be most suitable to young people who are not looking for a luxurious stay
Review submitted 17 Oct 2016
Kruger & Coast , September 2016
Katherine Ward
Really enjoyed this trip and the group was really friendly and got on well from the beginning. Meals were excellent and the campsites were all pretty good. Lots of opportunities for game drives - wished I was carrying on down to Cape Town.
Review submitted 13 Oct 2016
Kruger to Vic Falls , September 2016
Juliane Panhans
Travelling with Intrepid is always a good idea.
Review submitted 12 Oct 2016
Kruger to Vic Falls , September 2016
Susan Scott
Animals were great. Saw four of the big five. Travelled with great people. I could have done with a little bit more comfort but it was a camping holiday. Bus could do with a led light for nighttime cooking.
Review submitted 11 Oct 2016
Kruger to Vic Falls , September 2016
Cathrine Taylor
Be prepared for some long travel days but they are worth it. We were rewarded with up-close animal experiences and opportunities to interact with locals. Don't miss the Apartheid Museum in Johannesburg - an optional extra but a must-do.
Review submitted 09 Oct 2016
Kruger to Vic Falls , September 2016
jeffrey sweet
great time , bizzy. lots to see in a large land. thanks
Review submitted 08 Oct 2016
Vic Falls to Kruger , September 2016
Jacynta Krakouer
Intrepid pride themselves on providing culturally rich and ecologically sensitive experiences. Our guides/crew Justen and Gift were passionate about sharing their part of the world making the Vic Falls to Joburg tour truly memorable. From tracking a white rhino on the ground, meeting a chief and dancing with locals the tour is jam packed with experiences unlikely to be found with any other company. Highly recommended.
Review submitted 08 Oct 2016
Vic Falls to Kruger , September 2016
Ian Moore
If in doubt with this trip just do it, guide and driver were fantastic and every single day had a wow factor
Review submitted 07 Oct 2016
Kruger & Coast , August 2016
Adam Thomas
This trip was epic. We saw the Big Five and so much more!! Learned alot about the culture, food and people.
Review submitted 06 Oct 2016
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Divergence without difference: phylogenetics and taxonomy of Chlamydia resolved
Members of Chlamydiaceae have been extensively characterized by complete genome sequencing. This information provides new understanding concerning their natural evolutionary history. Comparative genome analysis is consistent with the conclusion that host-divergent strains of Chlamydiae are closely related biologically and ecologically. The previous taxonomic separation of the genus based on ribosomal sequences is neither consistent with the natural history of the organism revealed by genome comparisons, nor widely used by the Chlamydia research community 8 years after its introduction; thus, it is proposed to reunite the Chlamydiaceae into a single genus, Chlamydia.
- Chlamydia
- taxonomy
- genomes
The Chlamydia field operates deep within a research framework immersed in genomics. There are complete genome sequences representing the entire range of chlamydial organisms providing unprecedented depth and breadth for comparison. To date there are 16 publicly available genomes that can be accessed and analyzed with additional genomes forthcoming (Table 1). This is a rich resource that has defined broad new visions of chlamydial biology and engaged an influx of new researchers who continue to contribute to the chlamydial research arena. Evaluating the set of chlamydial genomes gives a global picture of the genome organization and biological capacity of each species, and reveals new insights into how they have become shaped during their evolution. Chlamydia sp. have been placed in their own bacterial division because they have an evolutionary pathway deeply separated from other bacteria (Pace, 1997). Significantly, most of the different species within Chlamydiaceae similarly separated from each other early in their evolution (Stephens, 2002) resulting in a very broad spectrum of pathoadaptive species (Pallen & Wren, 2007).
|Year completed
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|Sanger Institute
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|Yamaguchi University
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One inescapable perspective is that this wealth of genome-based knowledge reframes the reference for interpreting phylogenetic history and, moreover, recalibrates the set-point for a 16S rRNA gene-based molecular clock and consequent taxonomic interpretation (Coenye et al., 2005). In 1999, it was proposed to assign chlamydial strains in the single genus, Chlamydia, to two genera, Chlamydia and Chlamydophila, based on apparent differential clustering of 16S rRNA gene (Everett et al., 1999). Because the separation of strains was not robust and lacked other consistent biological markers, the proposal was rejected by many in the scientific community (Schachter et al., 2001). Today the field suffers from a lack of a cohesive taxonomy that continues to plague the Chlamydia research community. Unfortunately this has been maintained as dictated by a singular faith in 16S rRNA gene phylogenetics without respect for the biological system; especially, a biology so unusual that it is unlike the bacterial examples that served to validate the original concepts. The classic bacterial concepts for genus and species criteria are based on data derived from a small number of fast growing organisms that enjoy much genetic exchange, including DNA exchange between different species. Chlamydiae are not meaningfully fast growing and only rarely may they exchange DNA with other highly related Chlamydiae and only within the same intracellular vacuole (Demars et al., 2007).
Species and genus
A bacterial 'species' is defined as a distinct set of 'strains that have a close resemblance to one another' where 'each species differs considerably' (Brenner et al., 2005). For 16S rRNA gene, a sequence similarity of <97% often marks different species. At the genus level, separate species that cluster based on 16S rRNA gene sequence similarity and/or share the 'same general phenotypic characteristics' are grouped together (Brenner et al., 2005). Thus phenotype continues to serve a decisive role in decisions about cutoff points for 16S rRNA gene similarity at the species and genus levels (Stackebrandt et al., 2002).
The reason for using 16S rRNA gene similarity is that it serves as a common and convenient marker among bacteria from which one can infer relationships that ideally reflect biological and ecological similarities and differences. Thus when two strains differ with <97% similarity, it can be meaningful to infer separate species because there is the expectation that this will reflect concomitant biological divergence (Palys et al., 1997; Stackebrandt et al., 2002; Gevers et al., 2005). This is reasoned because sequence similarity and clustering is usually coincident with ecologically distinct populations as any adaptive advantage of one population would result in replacement of another population that resides in the same niche (Palys et al., 1997). The evolutionary processes that drive evolution of bacterial lineages are gene gain from the microbial gene pool, gene loss, gene order, and gene mutation (Pallen & Wren, 2007).
Everett (1999) cited Palys (1997) as the theoretical foundation to support the taxonomic splitting of Chlamydia based on 16S rRNA gene similarity clustering; however, this basis was misunderstood as Palys (1997) emphasized that there are exceptions. One notable exception is the unrestrained opportunity for neutral DNA sequence divergence for populations that live in the same environmental habitat, but are isolated geographically (Palys et al., 1997). In this case DNA sequences diverge because each isolated subpopulation cannot compete with each other nor exchange genes (Palys et al., 1997). Chlamydiae live in host-specific geographical islands, but otherwise live in the same ecological niche — that of the eukaryotic host, the eukaryotic host cell, and the inclusion vacuole. Living within the host cell, and especially within an inclusion vacuole, prevents Chlamydiae from having access to the exogenous microbial gene pool. Thus, speciation of chlamydial strains within a single genus Chlamydia is appropriate as each of the previously proposed Chlamydophila species is consistent with these fundamental principles –Chlamydia and Chlamydophila strains (1) cluster together, (2) often have <97% 16S rRNA gene sequence similarity, and (3) share all the fundamental and classically defined phenotypic characteristics.
How did this become distorted?
One can anticipate the origin of this confusion if two populations of strains become geographically isolated; their 16S rRNA gene sequences would then drift independently collecting unique mutations during geologic time. However, because each strain lives in an identical environmental niche with common selective pressures, there will be little biological change despite obtaining many nucleotide differences. In such a situation, inferences by percent similarity of 16S rRNA gene have very different levels of scale to be consistent with a taxonomy reflecting natural evolutionary relationships and cohesive differences in ecological niche. It may be convenient and even meaningful to link host adaptation to a species definition (Gevers et al., 2005), even if it will fail in some cases where Chlamydiae are found in multiple hosts (e.g. Chlamydia pneumoniae). Nevertheless, to establish a genus-level split at <95% 16S rRNA gene similarity (Everett et al., 1999) is arbitrary and scientifically untenable — independent of the previously elaborated inconsistencies (Schachter et al., 2001).
Gene print of evolution
How can genomic perspectives be used to resolve this mistake in nomenclature? Analyses of entire genomes are the ultimate measure of the similarities and differences in the biology and evolution of organisms as a genome documents the full natural history of the organism (Coenye et al., 2005). Two pertinent and fundamental observations can be immediately appreciated when comparing chlamydial genomes. The first observation is the reduction in genome size as a result of gene loss. Indeed, point mutation and gene loss may be the only mechanisms of divergence for Chlamydiae. This is apparent in 16S rRNA genes (Mira et al., 2001), and for Chlamydiae there has been the loss of multiple copies of 16S rRNA genes even to a single copy for some strains. Thus, adaptation to specific hosts has been dependent on minor mutation and gene loss rather than by the acquisition of new genes. The second observation is that the gene content and gene order (synteny) are extremely high. For almost every other bacteria, loss of gene synteny is one of the first changes to occur as strains diverge (Pallen & Wren, 2007). It is common that even between strains of the same species, there is virtually no discernable conservation of gene order beyond the operon (Coenye et al., 2005). The fact that there is nearly 80% conservation of genes and gene order between, for example, Chlamydia trachomatis, C. pneumoniae, and Chlamydia abortus is remarkable and reflects the common biological foundations of their intracellular niche, the loss of full systems for recombination (e.g. lack of RecG), and the lack of access to the microbial gene pool.
Developmental cycle limits evolution
Another essential biological process that profoundly affects the population structure and the evolution of Chlamydiae is their developmental cycle. The requirement to complete the cycle and produce infectious elementary bodies (EB) for strain propagation places a profound constraint on divergence of functional properties. Notably, the developmental cycle does not constrain neutral or even mildly deleterious mutations. In this case a biologically successful EB will often 'trap' neutral mutations that then become fixed in the population at higher rates than expected, analogous in some respects to the host bottleneck for endosymbionts (Moran, 1996).
Thus for Chlamydiae there are three important and fundamentally unique biological attributes that affect the appropriate understanding of simple counting of mutations in a single gene: (1) the bottleneck of the developmental cycle, (2) the geographic isolation within host cells and in different hosts, and (3) the paucity of horizontal gene transfer. All of these strongly bias the scale and, hence, interpretation of 16S rRNA gene differences between chlamydial strains.
Taxonomy and genomics
16S rRNA gene phylogenetic analyses demonstrating a split between the C. trachomatis group and all other Chlamydiae was always uncertain as it is supported by bootstrap analysis only 68% of the time (Everett et al., 1999). In independent studies that evaluated protein distribution based on complete chlamydial genome sequences, the distribution of proteins was not consistent with this early separation (Griffiths et al., 2006; Gupta & Griffiths, 2006). Similar inconsistency was observed when evaluating chlamydia-specific indels (Gupta & Griffiths, 2006). It is also noted that the chlamydial plasmid found in both proposed genera are very similar and the plasmid was likely acquired before their divergence (Griffiths et al., 2006). Analysis of the distribution of all encoded ORFs for C. trachomatis, C. pneumoniae, and Chlamydia caviae revealed one protein that is present in C. trachomatis and C. pneumoniae, but not in any of the other members of the 'Chlamydophila cluster' (Griffiths et al., 2006). This fact is inconsistent with two independent monophyletic branches. Moreover, only 20 proteins were unique for each of the two proposed genera and all of these were uncharacterized or hypothetical proteins (Inc proteins can be included in this category because we do not know their function). It can be seen that there are 89 protein differences among strains of Helicobacter pylori and over 200 differences for strains of Neisseria meningitidis (Lan & Reeves, 2000) — these are differences among strains of the same species. Given the 'sporadic distribution' of proteins across the chlamydial genera and unknown functions of these proteins, the biological and evolutionary interpretation of a meaningful difference is equivocal (Griffiths et al., 2006).
These findings challenge and undermine the validity of the proposal for separate genera. There is agreement that these chlamydial strains may be defined as different species and that there has been early divergence and separation of the chlamydial species during their evolution. This separation is often associated with isolation to a specific host. The most parsimonious and scientifically robust decision is that these are relationships consistent with species-level, not genus-level, affiliation.
Taxonomy resolved
The proposal by Everett (1999) set the boundary arbitrarily at <95% similarity in 16S rRNA gene as the threshold for deciding which of two genera chlamydial strains should be placed. It is inconsistent that this criterion is not met by most species (Fig. 1). Nevertheless, with several exceptions, they are all >97% similar, which is typically used for bacterial speciation, not genus-level demarcation. The deception is the apparent bifurcated clustering of chlamydial species by 16S rRNA gene sequence comparison; however, this is merely a biased outcome of their early separation in geologic time, their isolation in geographic host islands, and the evolutionary constraints imposed by the chlamydial developmental cycle.
A phylogenetic analysis based on 110 concatenated genes conserved in all Chlamydia genomes confirms and strengthens the close and linked evolutionary relationship among Chlamydiae (Fig. 2). In this tree, species trachomatis, muridarum, pneumoniae and pecorum cluster away from species felis, caviae, psittaci and abortus, further depreciating the value of 16S rRNA gene-based trees for the purpose of taxonomic classification in the Chlamydiaceae.
With genomics, we now understand the true evolution of Chlamydiae as they began their separation into different hosts 60–100 million years ago (Stephens, 2002), but remained true to the common constraints of their intracellular and intravacuolar niche. Today this natural history is reflected in the accumulation of mutations and gene loss while retaining their unifying biology. It can be confidently concluded that the spectrum of divergence among Chlamydiae is without generic difference.
It is of paramount importance to have a taxonomic nomenclature that is meaningful and useful for the research and medical communities, and not limited to the view promoted by rRNA systematics that disregards the unique biology of this system. The split of the genus was proposed over 8 years ago and still has not been accepted by the scientific community. This is easily and objectively established by the overwhelming majority of the publications that use only 'Chlamydia pneumoniae' (e.g. 81% in 2006) as the name vs. those that use only 'Chlamydophila pneumoniae' (16% in 2006). Such analysis demonstrates a tacit rejection of the two-genus nomenclature by researchers in the field, in spite of its inherent bias in favor of the two-genus system by neither including the quantitatively predominant research articles that include C. trachomatis in the title, nor accounting for reluctant authors who are required to use the 'official' two-genus system by editors. Unlike phylogeny, taxonomy is primarily a tool meant to facilitate analysis. The genus Chlamydophila is apparently still an unpractical tool for the vast majority of its users 8 years after it was first introduced. It is time now to end the taxonomic confusion by adopting the one-genus, multiple-species nomenclature (Fig. 1) that is both practical and scientifically justified.
Adapted with permission from Proceedings of the 6th Meeting of the European Society for Chlamydia Research, Aarhus, Denmark, July 1–4, 2008, Gunna Christiansen, Editor.
The authors sincerely thank S. Dela Cuesta, K. Hybiske, S. Abromaitis, S. Ruiz, and B. Hoft for their constructive and insightful criticisms and discussions concerning the content of this manuscript. This work was supported in part by NIH RO1 AI51472 (G.M. and P.M.B.).
Editor: Gunna Christiansen
- © 2009 Federation of European Microbiological Societies.


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Disney Family Sharon Mae Disney
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NORWALK - A Norwalk runner says he's excited at the prospect of taking part in the Boston Marathon again this year.
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Whipple's wife, Shannon, will be joining him by running in the marathon for the first time. After finishing last year in three hours and 15 minutes, Whipple says 45 minutes longer would have put him right in the vicinity of the bombs near the finish line.
He says he and his family were at a restaurant just a few blocks away. After learning of the explosions, Whipple's main goal was to get out of Boston immediately, fearing for the safety of his wife and two daughters.
He says it wasn't until he was back in Norwalk that the devastating chain of events truly sunk in.
Whipple's marathon run this year is training for a 100-mile endurance run in the summer. He hopes to beat last year's time by around 10 minutes.


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Investigator Joanna MacPherson is on probation … and one more screw-up could mean dismissal. She'll take on any case to prove herself—even the weirdest one she's ever seen. Lead investigator Cooper Maxwell has been tracking a group that assaults women, so the logical candidate to bait the trap is Joanna. Why not use one loose cannon to catch another?
Except that Joanna is too smart, too beautiful … and the last thing Cooper wants is to put her in harm's way.