text:
Settlement Grants Program
Upon exiting the HSS, entrants are referred to on-going settlement services provided through organisations funded under the Settlement Grants Program, and other relevant agencies.
The Settlement Grants Program funds services that provide assistance with:
- Orientation to Australia
- Developing communities
- Integration – inclusion and participation
For further information about the types of services funded under the Settlement Grants Program, please click here.
All HSS clients are eligible to receive assistance through the Settlement Grants Program upon exit, as well as any services complimentary to HSS services whilst still in the HSS.
For a list of services funded under Settlement Grants Program in your state or territory, please click here.

subset: commoncrawl


text:
A 2-year-old girl was shot with a BB gun on Saturday in Harlingen.
Harlingen police responded to a home on the 700 block of Jackson Ave. after the toddler TMs mother called to report the incident.
She told officers that a BB gun projectile struck her daughter TMs bare left foot.
A 18-year-old from across the alley, on Monroe Ave., was shooting his BB gun when one of the gun TMs projectile landed on the little girls foot.
Officers went to the home of 18-year-old Daniel Lopez. They confiscated the BB gun and kept it as evidence.
Lopez was placed under arrest for Injury to a Child and taken to the city jail.
His bond was set at $22,000.


text:
Jan 15th, 2007
Rolling Stone "Boss Hog"
Jeff Tietz from Rolling Stone Magazine wrote the following article focusing on the amount of pig shit produced by largest pork processor in the world. This article sheds light on the dirty side of bacon. This is from the December 2006 issue.
Smithfield Foods, the largest and most profitable pork processor in the world, killed 27 million hogs last year. That's a number worth considering. A slaughter-weight hog is fifty percent heavier than a person. The logistical challenge of processing that many pigs each year is roughly equivalent to butchering and boxing the entire human populations of New York, Los Angeles, Chicago, Houston, Philadelphia, Phoenix, San Antonio, San Diego, Dallas, San Jose, Detroit, Indianapolis, Jacksonville, San Francisco, Columbus, Austin, Memphis, Baltimore, Fort Worth, Charlotte, El Paso, Milwaukee, Seattle, Boston, Denver, Louisville, Washington, D.C., Nashville, Las Vegas, Portland, Oklahoma City and Tucson.
Smithfield Foods actually faces a more difficult task than transmogrifying the populations of America's thirty-two largest cities into edible packages of meat. Hogs produce three times more excrement than human beings do. The 500,000 pigs at a single Smithfield subsidiary in Utah generate more fecal matter each year than the 1.5 million inhabitants of Manhattan. The best estimates put Smithfield's total waste discharge at 26 million tons a year. That would fill four Yankee Stadiums. Even when divided among the many small pig production units that surround the company's slaughterhouses, that is not a containable amount.
continue at Rolling Stone (via regionalworkbench.org)
Author Information - During his spare time Eric writes for and maintains wannaveg.com. For his day job he works for an electronics company in San Diego, CA. Eric has been a vegetarian for about 10 years and believes that going 'green' and reducing meat consumption go hand-in-hand.
5 comments »
that's… amazing.
yeah…to say the least. It's pretty horrific IMHO.
although I'm still going to eat pork products, I'd have to say that make me feel bad…it is wrong in the eyes of some…but then some dont care.
Hi Miya, your comment is appreciated. Consciousness is the first step to action. You being aware and disheartened with the inhumane process of raising pigs for pork does make a difference. Spread the word whenever you feel it's appropriate, the more we all know, the more we can change.
Unfortunately the consumer's drive for lower cost food has led us to this sorry state. There is however an alternative to commercially farmed pork. Try searching specality butchers or delis to find what we in Ireland call "rare breed" pork products. This is meat from the older breeds which are slower growing but far superior in taste, usually reared outdoors so the animals have a more natural and happy life. I keep a few of these pigs (Tamworths) and supply family and friends so I like to think I am doing my bit in some small way to stand against the large corporations like Smithfield while at the same time ensuring at least some of the food I eat is ethically produced.


text:
We hand-craft responsive html for web design on cms & ecommerce sites.
Whether iPad, iPhone or Android we can build your multiplatform mobile apps.
We've got extensive experience building screen graphics for film and TV
Game development is our passion, we develop games for many platforms.
We developed this iOS and Android app for The Royal Australian and New Zealand College of Obstetricians and Gynaecologists.
We redesigned the ANU's most active web destination along with migrating thousands of articles and users to a new standalone domain.
We designed and integrated a new mobile responsive layout into an existing desktop website for Cancer Council Australia, with zero downtime.
A responsive and highly customisable website that allows for a continuously refreshed look and feel through new content.
An HTML5 gamification module for McDonald's restaurants to help engage staff during training for their new ordering system.
Responsive web design and development using a bespoke WordPress theme for built for desktop and mobile devices.
Web Developers
Canberra: (02) 6171 7500
Brisbane: (07) 5606 8122
We make use of all the most appropriate modern technologies and choose the right tool for the job considering the project perimeters. These include:
Javascript / JQuery
Adobe Air / AS3
Adobe Flash
PHP / My SQL
Expression Engine
And many more.
We provide high end web development, mobile app development, game development and screen graphics services for agencies, production houses, and film/TV throughout Australia and the UK.
e-Commerce web development
Photography portfolios
Fashion websites
Mobile apps
Screen graphics
Banner Ads
Mobile Games
Contact us to discuss your next project.
Providing outsourced developer services since 2008.


text:
Select a fulfillment store
for accurate stock availability
and pricing in you region.
You can change this at any time
Yes, let me select a store
Not now
To offer you a better experience, this site uses cookies. By continuing to use this site, you consent to the use of cookies.
For more information consult our Cookie Policy.
Enter your address to find closest fulfillment store or search by province, city or store name
Please note changing a fulfillment store may affect stock availability and pricing.
Create a new shopping list


text:
Catherine's work explores the visual experience of everyday urban life. Her paintings and drawings convey everything from abandoned buildings and old piers, to warehouses, bicycles and bubblegum machines. The common threads running through this seemingly diverse collection of subjects are utilitarian pragmatism, structural honesty, and an inadvertent beauty often arrived at through time and decay.
Latest News:
Open Studios
Urban Landscapes
Firehouse Arts Center
For more information:


text:
WASHINGTON - Capping one of his biggest legislative victories of his second term, President Bush yesterday signed a major overhaul of federal bankruptcy law, making it more difficult for Americans to wipe out their debts.
But until the new law goes into effect in six months, bankruptcy lawyers are expected to be filing about twice as many cases as normal, according to the American Bankruptcy Institute. That is because many people are rushing to file before the law changes.
The law says some of those who currently are able to file Chapter 7, which would allow them to wipe out debt they say they can't repay, will be pushed into Chapter 13, which means they will be given a judge-ordered monthly schedule for repaying their debt over three to five years.
The new law will make bankruptcy more costly, requiring those contemplating bankruptcy to pay for credit counseling before they go to court.
Prodded by the consumer credit-card industry and retailers, Congress has been toying with changing bankruptcy regulations for nearly a decade, but Mr. Bush made it a priority. The industry-sponsored provisions overwhelmingly passed the Senate and the House.
As Mr. Bush signed the bill, he said, "By restoring integrity to the bankruptcy process, this law will make our financial system stronger and better. By making the system fairer for creditors and debtors, we will ensure that more Americans can get access to affordable credit."
But consumer organizations overwhelmingly criticized the bill as unfair and harsh toward low-income families forced into bankruptcy by unplanned medical bills, job loss, or divorce. They especially criticized the move to force debt-ridden consumers into credit counseling.
The National Consumer Law Center and the Consumer Federation of America recently did a report on the large increase in credit counseling and found what it called an alarming rise in deceptive practices, excessive fees, improper advice and abuse of nonprofit status.
But the National Retail Federation said it welcomed the signing of the "long-awaited bankruptcy reform legislation." The federation complained that bankruptcy has changed from a stigma to a financial planning tool. "Every one of those filings means more bad debt that gets passed on to consumers, and consumers are tired of picking up the tab."
Mr. Bush agreed. "Bankruptcy should always be a last resort in our legal system. If someone does not pay his or her debts the rest of society ends up paying them." He added, "The act of Congress I sign today will protect those who legitimately need help, stop those who try to commit fraud, and bring greater stability and fairness to our financial system."
The new law imposes a means test that will make more families ineligible for the Chapter 7 plan to wipe out their debt and start over. A family with too much debt would have to file Chapter 13 - and pay off its debts - if it has income above the mean for their state, ranging from $45,867 in New Mexico to $82,561 in Massachusetts.
The number of bankruptcy filings is not insignificant, totaling 1.6 million a year. The bankruptcy institute has estimated that the new law may disqualify up to 200,000 from filing Chapter 7 and thus wiping out most of their debts in exchange for their assets, such as their homes. Others say it will keep as few as 30,000 families from wiping out their debts and starting over.
Contact Ann McFeatters at:
or 202-662-7071.


text:
The Messenger style bag is back with a bang this season, and this sophisticated urbanite version is just the thing for city slickers who want functionality as well as great looks. High tech canvas and Toro leather trim on the outside and a practical, Baby Ottoman water-resistant lining on the inside make this bag a winner.
At ECCO we are committed to make your online experience as easy as possible. The best way to make sure that your order is the right size is to measure your feet and then compare with the size chart below to find the appropriate size. Please follow these four simple steps to accurately measure your feet:
All you need to measure your feet from heel to toe is a piece of paper, a pencil and a ruler.
Place the paper on the floor up against a wall. Place the heel against the wall.
Mark the longest part of your foot on the paper. Repeat procedure with the other foot.
Measure the length of each mark and match it with the size chart. Let the longest foot decide the shoe size.


text:
In every issue, you'll find the hottest topics of interest to our readers along with cutting-edge health information.
America's 2 million long-haul truckers are entrusted with delivering our meds, clothing and food. Yet federal indifference to their work condi...
Kory Montoya, a New Mexico Apache, was diagnosed with HIV in 1991. Here he marks National Native HIV/AIDS Awareness Day, March 20, by telling...
A mealtime plan to handle diabetes and HIV in one easy serving
Trying to stop smoking? Ear magnets might work while a pill faces problems.
Can a cancer drug double as an HIV therapy?
A medical study has found benefit in something we like drinking: Downing about 2.2 cups of coffee a
Obsessing over the latest "superbug"? Relax: You can keep your body clean of infection.
How to manage your health insurance forms without a long paper trail
AIDS has affected my entire adult life. I'm pretty sure I've had HIV since 1986, when I
Tallying people who live with HIV can leave the community down for the count.
The CDC vs. the states in routine HIV testing
A Broadway strike muzzles HIV fund-raising efforts.
A gay African-American TV series takes sex in the city to a new, more HIV-savvy demo
The pink triangle vs. gun deaths
The University of Utah's student newspaper recently printed AIDS-related statements (see daily
A volunteer force of doctors, scientists and activists, AIDSTruth.org has been policing global media
In an era when half of all new HIV infections occur among people under 25, The Complete HIV/AIDS Tea
This past fall, ABC's court drama Boston Legal heralded the condom as "the single most i
A novel redraws HIV
Collin Burke, a high school senior from Madison, Wisconsin, says he took art class "by acciden
The Art in a Box program exports self-esteem to positive kids globally.
Annette Lizzul finds discrimination in the one place she thought she was safe.
FY 2008 Appropriations
A Religious ExperienceI must first commend POZ for an excellent November issue, especially the featu
Tribal Council


text:
Simply the Best Place to go for Online Fly Fishing and Fly Tying
Oct 25. 2016
(0 replies)
Oct 25. 2016
(0 replies)
Oct 25. 2016
(0 replies)
Oct 25. 2016
(0 replies)
Sep 28. 2016
Mike, Your questions are not stupid at...
Apr 17. 2016
Tim, What a good looking style! I love...
Jan 6. 2016
Dear Wiggy & all who just don'...
Nov 23. 2015
Miro Jagger's picture inspired me for a...
Published Sep 5. 2013 - 3 years ago
Nagli Spey Salmon Fly
Material Used;
Hook, Patriot CS16U/2 Up/Eyed Double size 8
Thread, Uni-8/0 Black
Rib, Silver Wire
Body, Blue Uni-Mylar Tinsel
Under-Wing, Pearl Krystal Flash
Wing, Dyed Black Fox
Spey Hackle, Whit eared Pheasant Dyed Teal Blue and Kingfisher Blue
Front Hackle Natural Guinea Fowl
Eyes, Jungle Cock
Originator's web site:
Martin Joergensen
Add new comment
Log in or register to pre-fill name on comments, add videos, user pictures and more.
Read more about why you should register.
Read more about why you should register.
ContactContact us
Contribute articles
No guest posts
The Global FlyFisher Newsletter
About the Global FlyFisher
Discussion forum
PoliciesCopyright © 1994-2016
Copyright © The Global FlyFisher
Copyright © contributing authors
No reprint without permission
- paper, electronic or otherwise
Copyright policy
Review policy
More policies


text:
Chores Magnet Board
By: Imaginisce
We are adding the craft to your Craft Projects.
The project was added to your Craft Projects.
Click here to view your Craft Projects.
Get the family on task with this decorative and functional Chores magnet board from Imaginisce. Create a photo magnet for every member of the family. This craft is also great for classroom assignments.
- 6 1/2 x 8 1/2" Good Morning Sunshine (aqua side) paper #001897
- Lucy Bird Bath Rub-ons #001909
- i-top Brad Maker Tool #001309
- 5-6 Medium Magnet Daddies
- 22" Sheer Brown 2" Ribbon
- 1" Chipboard Letters (source unknown)
- Printed Text on White Cardstock
- 8 x 10" Metal Sheet
- Family Photos printed on regular paper*
- Chestnut Roan chalk, Clearsnap
- Mod Podge (optional)
- Crystal Lacquer, Judikins (optional)
- The best place to find metal sheets cut to the size you'd like, is call around to your local heating/cooling places or sheet metal shops. Our shop even drilled the holes we needed at the top of the metal sheet for us and it cost about $1.80.
- Ink edges of all trimmed paper using the chestnut roan chalk ink.
- Adhere paper to center of metal sheet using
- Create photo magnets with printouts on regular paper. Photopaper is very thick and difficult to mold around the edge of the magnet shell. But if you'd like your magnets to shine like a photograph, consider coating them with Crystal Laquer from Judikins.
- Print out your desired "chores" onto white cardstock. Trim around words and ink with Chestnut Roan ink.
- Adhere text to board and place rub-ons (scalloped circle and bird) on metal sheet as shown or as desired.
- Adhere chipboard letters to top of board. If using mod podge, we would suggest coating entire sheet with mod podge once you have placed your text, rub-ons and chipboard letters on the metal sheet.
- Tie sheer ribbon through holes and hang where you'd like.
Tags / Related Topics
Your Recently Viewed Projects
Images from other crafters
Love these projects? Get MORE delivered to your Inbox – FREE Click Here!
sweetlyscrapped 11 71372
Jun 16, 2011
Adorable! I love this idea. I've been looking for something new and fun for our family's chore charts!
Member 3750563
May 03, 2010
Me encanto la idea, muchas gracias. Besitos.
Report Inappropriate Comment
Are you sure you would like to report this comment? It will be flagged for our moderators to take action.
Thank you for taking the time to improve the content on our site.
What's Hot
Something worth saving?
- SAVE all your favorite crafts
- ADD personal notes
- QUICKLY reference your crafts
Free eBooks
- 11 Crochet Shawl Patterns: Crochet Poncho Patterns, Free Easy Crochet Patterns and More
- 12 Knitted Scarf Patterns: Fabulous Free Knitting Patterns for Beginners
- 16 Free Crochet Hat Patterns, Scarves, and Gloves
- 31 Halloween Craft Projects
- 7 Adult Coloring Pages
- Homemade Halloween Decorations
- Recycled Craft Ideas: Easy Crafts to Make with Recycled Material
- 11 DIY Christmas Decorations and Gift Ideas
- 11 Teacher-Approved Crafts for School
- 12 Easter Egg Ideas & Easter Crafts for Adults
Our Newest Projects & Articles
- DIY Faux Fur Crossbody Bag
- Rustic Wedding in a Barn
- DIY Burlap Aisle Runner
- Shell N Picots Slouch Hat
- Recycled Pallet and Scrap Booking Shelf
- Stamped Matilda Inspired Hoodie
- Candy Corn Fabric Wreath
- Fall Burlap Wreath
- Big Knit Easy Blanket Sweater
- Pick Your State Printable Art


text:
Fact Checking Othmar
Who is Othmar Vohringer? The Bodock Times has undertaken a monumental study to determine what secrets he is hiding. Says he is German, speaks like a New Zealander, wears a hat like an Aussie, and why is he hiding out in Canada? A secret study in Acronyms has come to a startling discovery. First of all we know that ringer is a fake and if you take the rest of his name jumble it up, take out a few letters, then add some, you come up with Danger Man. If you do it again you come up with James Bond 007.
Labels: Funny Videos/Songs


text:
Training and learning for the future: making change happen
- Correspondence to Dr Bob E Klaber, Imperial College Healthcare NHS Trust St Mary's Hospital, The Bays Building, 16 South Warf Road, London W2 1PF, UK; Robert.Klaber{at}imperial.nhs.uk
- Accepted 28 February 2014
Healthcare is changing. There are increasing numbers of people with multiple comorbidities, ageing populations, stark health inequalities and rising patient expectations about quality of care. As access to personal healthcare data, technological advances and changes to patients' expectations as to how information is shared1 continue to develop rapidly, healthcare systems across the world are struggling to adapt.
There are also significant concerns about the ability of the global healthcare workforce to meet these demands. The potential impact of a health workforce crisis is greatest in emerging economies. However, there is a strong argument that more advanced economies also need to respond, both to the impact of economic austerity and to the combined problem of an ageing population and ageing health workforce. Buchan and Campbell suggest that as new models of care emerge this is both an opportunity for transformative thinking and a threat to the status quo.2 They argue that the aspiration for universal health coverage will not be achieved without a shared commitment to a transformative health workforce agenda across the globe. Across all countries and in all health systems there is "no health without a workforce".
If healthcare is changing, education and training programmes need to adapt and change too, so that a future workforce can deliver high quality care in these new settings. In …


text:
Photo © Gary Watt - AirTeamImages
Photo © Robert Unwin
Photo © Marlo Plate - Iberian Spotters
Photo © Oscar Bernardi
Photo © Douglas Olsen
Photo © Willam W. Sierra
|Quoting JRadier (Reply 1):
We still have Helijet in Canada, flying between Victoria Harbour and Vancouver Harbour (it's core route, they fly a lot more)
|Quoting Fanofjets (Reply 14):
|Quoting Fanofjets (Reply 14):
There was another airline, Island Helicopters, that flew local tours. I believe they are now Liberty Tours.
|Quoting Isitsafenow (Reply 8):
You had em in the late 50's and into the 60's.
CHA Chicago Helicopter Airlines
LA Airlines
New York Helicopter Air
Those were the majors in the USA
|Quoting TomTurner (Reply 19):
believe there was an operator SFO-OAK as well.
|Quoting TomTurner (Reply 19):
I believe there was an operator SFO-OAK as well.
Photo © Mel Lawrence
Photo © Mel Lawrence
|Quoting Fanofjets (Reply 14):
Ah, back in the 1960s and 1970s, helocopters actually landed on the roof of the Pan Am building in Manhattan. After a crash in the late 1970s, this practice was abandoned.
|Quoting Ikramerica (Reply 22):
Are there any helicopter airlines still left in Hawai'i


text:
25 years experience. Corrective and therapeutic shoeing! I will call you back and I will show up! Quality work, experience that counts!!
SE Ok and NW AR, will travel further for larger numbers.
From the trails to the performance arena, we can help you go to the next level. We offer lessons for all levels and ages, we often have lesson horses on hand for sale, and we train horses. Danny has also been a farrier for 25+ years and specailizes in corrective and balance issues. Please give us a hollar if your in need of anything, we'll be happy to help! Recently moved to the Spiro Ok. area.... (read more)
Avoid Scams and Fraud - Potential areas of fraud: wire transfer, moneygrams, money orders, cashier checks, shipping, escrow, "transaction protection", "guarantee". Check out the Buyer Safety section for more info.


text:
America - From sea to shining sea and from inner city to suburb... we are one.
you #$%$
what planet are you from
Sentiment: Sell
Planet Kobel. look it up on google.
This is so typical liberal think. It's so effeminate to think that everyone getting along is progress. There's a great quote, "in Italy, for thirty years under the Borgias, they had warfare, terror, murder and bloodshed, but they produced Michelangelo, Leonardo da Vinci and the Renaissance. In Switzerland, they had brotherly love, they had five hundred years of democracy and peace – and what did that produce? The cuckoo clock."
It's not liberal think it's common sense and respect for all Americans. The minute someone puts their race in front of American when describing oneself makes them a racist.
Riots coming if my hommie dont win....you better run whitey
you pinhead... why don't you put your computer down and pick up a book
I call myself a Responsible American all the time and now you suggest I am racist???? I beg your pardon but you don't even know me!
this is good message for the bigots on this board to read ! A few pretend to being black with their bigot topics !


text:
Welfare Reform:
Child Support an Uncertain Income Supplement for Families Leaving Welfare
HEHS-98-168: Published: Aug 3, 1998. Publicly Released: Aug 3, 1998.
Additional Materials:
- Full Report:
(202) 512-7202
Office of Public Affairs
(202) 512-4800
Pursuant to a congressional request, GAO provided information on how successful states are likely to be in obtaining child support for families whose benefits are subject to time limits, focusing on: (1) how successful states that experimented with time-limited benefits before welfare reform have been in obtaining child support for families who reach their limits; (2) how successful states have been in obtaining child support for families within a 5-year period, the maximum time a family may receive Temporary Assistance for Needy Families (TANF) benefits; and (3) the implications time limits have for states and families.
GAO noted that: (1) many TANF families may not be able to count on child support as a steady source of income when their time-limited welfare benefits expire; (2) in the first three states to enforce welfare benefit time limits--Connecticut, Florida, and Virginia--only about 20 to 30 percent of families had any child support collected for them in the 12 months before their welfare benefits were terminated; (3) about one-half or more of the child support cases without collections lacked a child support order legally obligating a noncustodial parent to pay child support at the time the families' assistance was terminated, despite having a long history in the child support program before time limits were implemented; (4) for families whose child support was secured, the median collections among the three states ranged from a total of $581 to $1,348 for the 12-month period; (5) in two high-performing child support states, Minnesota and Washington, GAO observed better outcomes for a sample of Aid to Families with Dependent Children child support cases that first opened in 1992 and remained open for 5 years; (6) about two-thirds of the families received some child support in the last 12 months of that period; (7) support order establishment rates were higher for these cases as well: in both states, orders were established within 5 years for more than 80 percent of the cases that needed them; (8) the median amounts of child support collected for these families ranged from $1,875 to $2,118 for the 12-month period; (9) despite these outcomes, about one-third of the child support clients in these states reached the end of the 5-year period without any child support; (10) to better ensure that child support is available for families in a time-limited welfare system, states will need to improve their child support performance for families already in the welfare system and for those who enter it for the first time; (11) in the three states GAO studied that had imposed time limits on families already receiving aid, from one-half to three-quarters of the families could not get child support because the state did not or could not locate the noncustodial parent; (12) it is also important for states to move quickly to pursue child support for families that have just begun receiving aid; (13) state officials told GAO that information on noncustodial parents is best pursued early and aggressively to achieve successful outcomes; and (14) GAO's analysis showed that successful outcomes are most likely within 2 years after a family begins receiving child support services.
Sep 15, 2016
Supplemental Security Income:SSA Provides Benefits to Multiple Recipient Households but Needs System Changes to Improve Claims ManagementGAO-16-674: Published: Aug 17, 2016. Publicly Released: Sep 15, 2016.
Sep 7, 2016
Foster Care:Most Tribes Do Not Anticipate Challenges with Case Goal Changes, but HHS Could Further Promote Guardianship AssistanceGAO-16-625: Published: Aug 8, 2016. Publicly Released: Sep 7, 2016.
Jul 8, 2016
Supplemental Nutrition Assistance Program:Policy Changes and Calculation Methods Likely Affect Improper Payment Rates, and USDA Is Taking Steps to Help Address Recipient Fraud [Reissued on July 8, 2016]GAO-16-708T: Published: Jul 6, 2016. Publicly Released: Jul 6, 2016.
Mar 14, 2016
Social Security Disability:SSA Could Increase Savings by Refining Its Selection of Cases for Disability ReviewGAO-16-250: Published: Feb 11, 2016. Publicly Released: Mar 14, 2016.
Mar 10, 2016
Temporary Assistance for Needy Families:Update on States Counting Third-Party Expenditures toward Maintenance of Effort RequirementsGAO-16-315: Published: Feb 10, 2016. Publicly Released: Mar 10, 2016.
Feb 23, 2016
Unaccompanied Children:HHS Can Improve Monitoring of Their CareGAO-16-429T: Published: Feb 23, 2016. Publicly Released: Feb 23, 2016.
Nov 9, 2015
Foster Care:HHS Could Do More to Support States' Efforts to Keep Children in Family-Based CareGAO-16-85: Published: Oct 9, 2015. Publicly Released: Nov 9, 2015.
Sep 16, 2015
Child Welfare:Steps Have Been Taken to Address Unregulated Custody Transfers of Adopted ChildrenGAO-15-733: Published: Sep 16, 2015. Publicly Released: Sep 16, 2015.
Jun 15, 2015
Older Americans Act:Updated Information on Unmet Need for ServicesGAO-15-601R: Published: Jun 10, 2015. Publicly Released: Jun 15, 2015.
Jun 3, 2015
Supplemental Security Income:An Overview of Program Integrity and Management ChallengesGAO-15-632T: Published: Jun 3, 2015. Publicly Released: Jun 3, 2015.
Looking for more? Browse all our products here


text:
This is a shot from my new business cards I just got printed for my personal branding.
The back pattern is based off my my 3 great grand father's weave patterns.
keyboard shortcuts:
L or F like
post comment
Show and tell for designers
What are you working on? Dribbble is a community of designers sharing screenshots of their work, process, and projects.
Copyright © 2009–2016 Dribbble LLC. All screenshots © their respective owners. Shipped from Salem, Mass. USA.


text:
Welcome to the Bulverde/Spring Branch Library Catalog!
Electronic Resources
Sorry, access to electronic resources is suspended until issues are resolved. Please see My Account for details.
OverDrive - Lone Star Digital Library Login Needed
Downloadable books, audiobooks, music, and video
OneClickDigital Login Needed
On-line access to downloadable MP3 and WMA audiobooks: all iPod compatible.
Rocket Languages Login Needed
Learn a Language with Rocket Languages. We offer 11 different foreign language courses.
AtoZdatabases Login Needed
The Premier Marketing and Reference Database.
Zinio Login Needed
Full digital copies of your favorite magazines.
NoveList Login Needed
Find a good book to read! NoveList allows you to search for your favorite titles and authors in order to locate similar works of fiction. Novelist also allows you to search by keyword for subjects you're interested in reading about. The database then provides a comprehensive list of titles, series information, reviews, and book discussion guides.
ArtistWorks Login Needed
Unlimited access to beginning to advanced lessons for instruments, voice and physical art.
TexShare Databases Login Needed
Includes thousands of full-text magazines and newspapers, WorldCat (35 million library records), Science and Literature databases, on-line encyclopedias, etc.
Ancestry.com Library Edition (in-library use only) Login Needed
A comprehensive online source of information for conducting genealogical research.
HeritageQuest Login Needed
HeritageQuest genealogy resource
Learning Express 3.0 Login Needed
New version! Review skills and prepare for tests.
Home Improvement Reference Center Login Needed
HIRC provides detailed, user-friendly "how-to" information covering all manner of home improvement/repair projects. HIRC features full-text content from leading home improvement magazines, images not found anywhere else online, and videos of popular home repair projects.
Hobbies and Crafts Reference Center Login Needed
Want to try a new craft or learn more about your current hobby?- dig in!
Small Business Reference Center Login Needed
Information on small business and entrepreneurial subject areas, common business types, a help and advice section, and information on how to create business plans that lead to successful funding.
AR BookFinder Login Needed
Searching for Accelerated Reader books is fun and easy with this free online tool.
DearReader.com Online Book Club Login Needed
Sign up to receive chapters from popular books in your daily email. Choose more chapters during the week, enough to know if the book is one you'll want to finish. Features fiction, nonfiction, business, teen romance and mystery titles.
Learning Express 3 - Computer Skills Center Login Needed
Video courses are the fastest and easiest way to learn how to use a computer. Use the materials in this center to learn the basics of a computer, from searching the Internet to using popular programs for word processing, creating spreadsheets and databases, implementing design, and more.
Learning Express 3 - Career Center Login Needed
Are you preparing for a professional exam? Are you curious about a particular occupation or looking to improve your workplace skills? Begin exploring your future here.
Learning Express 3 - Adult Learning Center Login Needed
This center offers busy adults the resources to improve their basic skills, earn a GED Test credential, or become a U.S. citizen.
Learning Express 3 - High School Equivalency Center Login Needed
Earning a high school equivalency credential shows that you have high-school-level academic skills. It can help open the door to a better job or career, and a college education. The resources in this Center can help you every step of the way, from finding out if you're ready to take the test, to building your academic skills, to preparing for your high school equivalency exam.
Learning Express 3 - Recursos para hispanohablantes Login Needed
¿Le gustaría practicar más su lectura y escritura en inglés? ¿Busca mejorar o refrescar sus habilidades matemáticas? Cada curso de aprendizaje y las pruebas de práctica calificados instantáneamente en este Centro de Aprendizaje tienen instrucciones detalladas en español que le ayudarán a alcanzar sus metas personales.
Learning Express 3 - College Center Login Needed
If you need to strengthen your fundamental academic skills for your courses, prepare for an important placement test, or ensure a top score on a graduate school entrance exam, the resources here can help you reach your goal.
Learning Express 3 - School Center (Texas) Login Needed
The resources here can help students prepare for important tests, get extra help with assignments, and boost their skills in important subjects.
Learning Express 3 - Job and Career Accelerator Login Needed
Powerful tools and expert guidance to help you find a great job.
LibriVox Login Needed
Public domain recordings of public domain literature. Plays anywhere!
Project Gutenberg Login Needed
Want 30,000 free ebooks? Download to your own device using PDFs, HTML, epub, Mobipocket and more. Enjoy!
International Children's Digital Library Foundation Login Needed
Outstanding multilingual historical and contemporary books from throughout the world.
What's Hot
|Tote Bag
|Bullseye (Michael Bennett mysteries, #9)
|The Chicken Squad : the first misadventure (Chicken Squad, #1)
|DVDDespicable me 2
|DVDThe peanuts movie
|Missing (Private series, #12)
|The games (Private series, #12)
|Home (Myron Bolitar Novel, #11)
|DVDMr. Right
|DVDLondon has fallen
|DVDS.T.E.M. : Vol. 2
|Extreme prey (Lucas Davenport series, #26) (Prey series, #26)
|Mesmerized: how Ben Franklin solved a mystery that baffled all of France
|DVDThor : the dark world
|DVDHotel Transylvania
|Woman of God
|Harry Potter and the cursed child : Parts one and two
|Bullseye (Michael Bennett mysteries, #9)
|Smooth operator (Teddy Fay novel)
|Smooth operator (Teddy Fay novel)
|DVDThe Boss
|DVDAllegiant (Divergent series)
|Night and day (Eve Duncan novel, #21)
|DVDDear Frankie
|DVDThe dog lover
|The black widow (Gabriel Allon series, #16)
|Banned Books — 21 titles
|Bluebonnet Nominees 2015-2016 — 20 titles
|Business Resources — 41 titles
|Caldecott Winners — 44 titles
|Distinguished Texans — 39 titles
|Kindle Preloaded Titles — 33 titles
|Leadercast — 12 titles
|Newbery Medal Award Winners — 81 titles


text:
CBS Paramount adds series
CBS Paramount International Television continues to expand its roster of programming with series from sister division Showtime.
CBS Paramount International Television continues to expand its roster of programming with series from sister division Showtime.
Spanish terrestrial broadcaster Cuatro continues to put its faith in U.S. series, inking with Lionsgate, Paramount, CBS, Universal, HBO and BVI for a raft of skeins.
Spain's BRB Intl. will announce at NATPE that it is moving into production on a second season of its popular paunchy polar bear toon "Bernard."
In a last-minute announcement on the eve of NATPE, Warner Bros. took the wraps off a multiyear deal with the Fox O&Os for a daily strip based on its Web sensation "TMZ."
Four key TV execs in varied parts of the biz received the Brandon Tartikoff Legacy Awards Monday evening in Las Vegas on the eve of NATPE.
BCI, a wholly owned subsid of Navarre Corp., has licensed the long-term North American DVD publishing and distribution rights to programming from FremantleMedia's portfolio of TV shows and films…
Endemol U.K.'s managing director of digital media Peter Cowley kicked off NATPE Mobile on Monday with a keynote address highlighting his company's strategy for generating profits in the digital…
AMC's showbiz newsmag "Sunday Morning Shootout" is being shopped to local stations here at NATPE and is already close to tying up a deal in its first major market -- appropriately enough, L.A.
Roma Khanna, senior VP of content at Canada's Chum TV, has been nominated as co-chair of NATPE, the nonprofit group that organizes the annual syndie tradeshow.
Twentieth TV has renewed its three courtshows -- "Divorce Court," "Judge Alex" and "Cristina's Court" -- for the coming season. Deals were unveiled Monday in Vegas by Twentieth prexy-chief operating…
"American Idol" will launch a mobile venture simultaneous with the start of its sixth season on Jan. 16, Fremantle Media exec VP of licensing worldwide Keith Hindle announced Monday at NATPE Mobile.


text:
casino royale games machine download NaYR free file
== Search results for casino royale games machine ==
Searched file: casino royale games machine
*Filename: casino royale games machine.zip
*Year: 2015
*Total Downloads: 33757
*Uploaded: 02/22/2015 21:14:59
*Type: WinZip
*Supported OS: All
== Searches related to casino royale games machine: ==
02/22/2015 21:14:59
Totally free Royal Reels slot machine. Play more than 1050 free casino games in your browser. Slots, video poker, blackjack, roulette & more.Awesome Fupa Games flash arcade site with thousands of free online games.Games available in most casinos are commonly called casino games. In a casino game, the players gamble casino chips on various possible random outcomes or ...Casino Royale is the twenty-first film in the Eon Productions James Bond film series and the first to star Daniel Craig as the fictional MI6 agent James Bond.Award-winning casino entertainment. Royal Vegas Online Casino brings you a world of exclusive casino entertainment with a massive selection of casino games and ...Casino Bonus Offers. Casino Kings treats all of our players like royalty with casino bonusoffers fit for a King. The royal treatment begins right away with our ...Online Casino Games . Superior Casino offers a wide variety of casino games. Classics like Blackjack, Slots, Roulette, Video Poker and Craps are found here plus ...Casino Royale in Maho Village is the largest on St Maarten. With 20 tables of gaming, you can choose from roulette, craps, baccarat, blackjack and poker. The 450 slot ...Play casino gmaes at Royal Ace Casino and get $1000 welcome bonus. Royal Ace Casino brings gaming luxury with finest collection of casino games. With over 100 casino ...7Red.com is Here and Ready for Your Action! Get swept up in the fast-paced, exciting games at 7Red.com! Sizzling blackjack, roulette, slots machines, poker and more ...
casino royale games machine
== Related Links: ==
ubuntu i386 vs amd64 wii backup games buy how does ebay automobile shipping work fhm sexiest 2014 return to vendor process audit programs webcam plug play no drivers x83 printer drivers ryoko hirosue mp3 mp3 quilt block nine-patch wiggle and giggle fhmonline games powered by vbulletin the flash cw 2014 rossa feat ungu mp3 penis une question de taille how to beat the rancor in kotor hp installation instruction dead can dance black sun mp3 business pro 500c osx drivers dark knight returns comic pdf deer hunter 2 cheat codes pc definition full employment mdp3900v u modem driver seeing eye dog programs california rejects the hangover bit torrent Crazy, Stupid, Love. (2011) t and t s01 download pirated mac os x trackball explorer vista driver kenmore model 110 disassembly instructions raining man deutsch mp3


text:
Classic Group is proud to present AkthubaCityPark, the biggest real estate development project in the Volgograd Region.
With over 1 million square meters of residential real estate, 200,000 square meters of commercial facilities, and a broad range of social infrastructure, the project is set to become a reference in integrated urban development in the South of Russia.
Designed with leading Western European Bureaus, the project will provide over 40,000 inhabitants with a superior living experience.
Beyond its ideal location, AkthubaCityPark combines the concepts of environment-friendly design and construction, with high quality yet affordable mixed use buildings of various typologies.
Distributed across different clusters, the constructions harmoniously create an ideal and self-contained living environment that alternates living spaces with green areas, social infrastructure, as well as commercial and leisure-oriented premises.
Volzhsky is a major industrial city with a growing population of over 300,000 inhabitants. It is located on the east bank of the Volga River, 20 kilometers northeast of Volgograd. In 2015, a new bridge and motorway will link Volzhsky to Volgograd, reducing the travel time between the centers of the two cities to about 20 minutes, from the current 60 minutes.
Compared to Volgograd, AkthubaCityPark and Volzhsky represent a greener alternative that combines all types of urban facilities with the more comfortable and family-oriented aspects of a smaller town, inspired by Western European integrated urban planning.
The design of the district breaks away from traditional Russian master plans, thanks also to the collaboration with the Dutch firm KCAP Architects and Planners, a well-known European architectural and urban planning bureau.
The construction itself leverages environment-friendly technologies and materials that are of higher quality and durability, especially if compared to other ongoing constructions within the same area. Thanks to Classic Group's expertise, the end result is a superior product that the local middle-class is able to afford and to enjoy for many generations to come.
AkthubaCityPark provides a wide range of social infrastructure objects: it integrates eight kindergartens, three schools , equipped with modern swimming pools and sports facilities, and numerous green walking and resting facilities. This type of integrated planning generates a higher sense of "belonging" and "identity" that invites for a higher respect of common areas.
AkthubaCityPark also offers top-quality commercial real estate that is developed by leveraging the expert advice of local market research companies and Knight Frank, a global leader in commercial real estate consulting. The result is a mix of commercial infrastructure that answers both internal (inhabitants) and external demand. This translates into premises such as a Commercial Centre, Hotels, Expo and Office Centers, together with retail premises, fitness centers, private medical clinics, etc.


text:
Made from 4 pound cakes and Buttercream icing. I used Backyardigan bobble head circus toys on each car and caboose and a #2 candle for the smoke stack. Wheels are Oreos and Lifesavers. Windows are Lifesavers as well. This was a fun cake!
great idea...so cute! love the wheels!!
CakeCentral.com is the world's largest cake community for cake decorating professionals and enthusiasts.
(if this is a private computer)
Yes please, register now!


text:
35 posts tagged with bribery.
Displaying 1 through 35 of 35. Subscribe:
The Bribe Factory
The Company That Bribed The World - It was the company with jet-set style and dirty hands. From the tiny principality of Monaco, Unaoil reached across the globe to pay multi-million dollar bribes in oil rich states. The beneficiaries? Some of the biggest companies in England, Europe, America and Australia.
Ashley Madison has been hacked.
Cheating website AshleyMadison has reportedly been hacked. Besides snippets of account data apparently sampled at random from among some 40 million users across ALM's trio of properties, the hackers leaked maps of internal company servers, employee network account information, company bank account data and salary information. They want the site taken down, not because of the cheating, but because the company offers a "total delete" function and charges for it, but doesn't actually do it.
I could've had class. I could've been a contender...
Ron Paul's 2012 campaign implicated in bribery scandal. On the eve of the 2012 Republican Iowa Caucuses, US Rep. Michele Bachmann alleged that Iowa State Senator Kent Sorenson informed her that he had switched his support to Rep. Ron Paul's presidential campaign after being promised a large sum of money. Ron Paul personally denied these claims, but emails and documents were recently leaked, with proposals for the Ron Paul campaign to pay up to $208,000 to Sorenson, his staffers, and his PAC. More damning, a recorded phone call has been released, in which Sorenson admits to being bribed, and implicates Jesse Benton, Ron Paul's former campaign manager, 2010 campaign manager for Rand Paul, and now, the manager of Republican Senate Minority Leader Mitch McConnell's 2014 campaign.
Bruised and Blattered: How Fifa's great survivor has stayed on top
"On Sunday, Joseph S Blatter attended a ceremony on the Indian Ocean island of Mauritius to celebrate the renaming of the country's FA headquarters in his honour. The Fifa president would perhaps say it was a fitting tribute, given his promotion of African football and the amount of "development" money poured into the continent over recent decades. His critics would say it was typical of his egomania and note the importance of African votes in keeping him atop world football for 15 years." [more inside]
The Bribery Aisle
"they were never meant to be smoked in the first place."
Cigarettes: The Most Stable International Currency. In China, expensive cigarettes (not to be confused with counterfeits of popular brands) are sometimes used as bribes. Cash can be difficult to handle, or outright illegal, in some places. Since a smoking ban (and subsequent black-market trade in cigarettes) in US prisons, canned mackerel (previously on MetaFilter) has become the exchange medium of choice. [more inside]
Crowd and Funding on Internet
Ben Kuchera, a video games journalist who has written for Wired, Ars Technica, and now the Penny Arcade Report, discusses the seedy underbelly of Kickstarter promotion.
"The biggest company you've never heard of."
"A Giant Among Giants[FP]: Glencore -- founded by famous fugitive Marc Rich [Salon] -- has cornered the market on just about everything. Now that it's going public, will its ties to dictators and spies stand up to scrutiny?" [more inside]
Match fixing in Soccer
"The World's most popular game is also its most corrupt, with investigations into match fixing ongoing in more than 25 countries. Here's a mere sampling of events since the beginning of last year: Operation Last Bet rocked the Italian Football Federation, with 22 clubs and 52 players awaiting trial for fixing matches; the Zimbabwe Football Association banned 80 players from its national-team selection due to similar accusations; Lu Jun, the first Chinese referee of a World Cup match, was sentenced to five and a half years in prison for taking more than $128,000 in bribes to fix outcomes in the Chinese Super League; prosecutors charged 57 people with match fixing in the South Korean K-League, four of whom later died in suspected suicides; the team director of second-division Hungarian club REAC Budapest jumped off a building after six of his players were arrested for fixing games; and in an under-21 friendly, Turkmenistan reportedly beat Maldives 3-2 in a "ghost match" -- neither country knew about the contest because it never actually happened, yet bookmakers still took action and fixers still profited." [All the world is staged: Bribed players, fake games. Criminal syndicates can fix any match, anywhere.]
The News Corporation scandals
Murdoch's Scandal - Lowell Bergman (the journalist portrayed by Al Pacino in The Insider) has investigated News Corporation for PBS Frontline [transcript]. He depicts Rupert Murdoch's British operation as a criminal enterprise, routinely hacking the voicemail and computers of innocent people, and using bribery and coercion to infiltrate police and government over decades. Enemies are ruthlessly "monstered" by the tabloids. Bergman also spoke to NPR's Fresh Air [transcript]. But the hits keep coming: in recent days News Corp has been accused of hacking rival pay TV services and promoting pirated receiver cards in both the UK and Australia. With the looming possibility of prosecution under America's Foreign Corrupt Practices Act, how long will shareholders consider Rupert Murdoch irreplaceable? [Previous 1 2 3 4]
Harry Redknapp, Rube of the Year
Despite evidence of extensive misconduct, English football coach Harry Redknapp remains beloved in the hearts and minds of football fans.
The Fall of the House of Murdoch?
Five senior journalists and editors at the News International tabloid the Sun were arrested on Saturday along with three public officials as Operation Elveden, the British investigation into bribery of police by News International papers, broadened to include corruption of officials in the armed forces and Ministry of Defence as well. The Guardian reports that the new arrests escalate the stakes of the ongoing US Foreign Corrupt Practices Act investigation of News Corporation, which carries potential penalties of millions of dollars of fines and prison sentences for senior executives. [more inside]
I am surprised they have the time, what with all the mustache twirling and damsel distressing
Bloomberg markets report Koch Brothers Flout Law - Getting Richer With Secret Iran Sales. Apparently, they knew this story was coming and began pre-butting it last week.
Traffic Drives Nigerians Nuts, but a Trip to a Shrink May Go Too Far
People often think that other drivers are nuts. The Nigerian authorities have taken things a step further, now requiring drivers accused of going the wrong way down a one way street to get psychiatric exams.
We have to wean fish off water
"The paper puts forward a small but novel idea of how we can cut down the incidence of bribery. There are different kinds of bribes and what this paper is concerned with are bribes that people often have to give to get what they are legally entitled to. I shall call these 'harassment bribes'. Suppose an income tax refund is held back from a taxpayer till he pays some cash to the officer. Suppose government allots subsidized land to a person but when the person goes to get her paperwork done and receive documents for this land, she is asked to pay a hefty bribe. These are all illustrations of harassment bribes. Harassment bribery is widespread in India and it plays a large role in breeding inefficiency and has a corrosive effect on civil society. The central message of this paper is that we should declare the act of giving a bribe in all such cases as legitimate activity [PDF]. In other words the giver of a harassment bribe should have full immunity from any punitive action by the state." [more inside]
Paying Zero for Public Services
Protesting corruption with the zero-rupee note. Indian NGO 5th Pillar has a come up with a unique project to help India's poor fight against institutionalized bribery.
Arms dealers affiliated with 22 companies, including one with a former Joint Chief of Staff on its board of directors, have been indicted for soliciting kickbacks on multimillion dollar deals to supply munitions to representatives of a fictitious African government. [more inside]
The mysterious death of a young hotel chef in China mobilizes thousands
"At 1am on June 19, police and funeral cars arrived at the hotel, wanting to take the body away. 2,000 Shishou residents blocked the hotel entrance to protect Xu's corpse."
SEO can't save you now
"Your article is ranking very well for term 'Cash4Gold'. Is it worth a few thousand to take it down?" [more inside]
Pocketful of dough
Pocketful of dough - an article on where the art of, er, tipping up front can get you. Originally printed in a year 2000 edition of Gourmet. Via Juicy Tidbits.
In search of Aids
Manipur, which has a population of 2,388,634, has the highest rate of HIV in the country, which is also the reason why it has the most number of NGOs working in the area. However, what is disturbing is that a day or two ago, one of these NGOs bribed a group of children into getting their blood tested, so that they could increase their chances of garnering more funds.
How Much is a Seat on the Security Council Worth? Foreign Aid and Bribery at the United Nations
How Much is a Seat on the Security Council Worth? Foreign Aid and Bribery at the United Nations. A paper from a doctoral student at the Harvard Business School, and an employee of the National Bureau of Economic Research has found a correlation between serving on the United Nations Security Council, and the amount of aid received from the United States and the UN. The paper will be printed in an upcoming edition of the Journal of Political Economy. From the abstract: "Ten of the fifteen seats on the U.N. Security Council are held by rotating members serving two-year terms. We find that a country's U.S. aid increases by 59 percent and its U.N. aid by 8 percent when it rotates onto the council. This effect increases during years in which key diplomatic events take place (when members' votes should be especially valuable) and the timing of the effect closely tracks a country's election to, and exit from, the council. Finally, the U.N. results appear to be driven by UNICEF, an organization over which the United States has historically exerted great control." The Harvard Business School working paper can be found here. Commentary from Steven Levitt (the co-author of Freakonomics) can be found here.
Nigerian Football Association Gives the Green Light to Bribery
Let me make you an offer you can refuse... The Nigerian Football Association has adopted the "if you can't beat 'em join 'em" policy regarding bribery of officials. They now say that officials can accept bribes, so long as said bribes don't affect the game's outcome. Brilliant. Why didn't anyone think of this before?
Pentagon bribery scandal -- Iraqi journalists bought out.
Pentagon bribery scandal -- Iraqi journalists bought out. Officials in Washington have admitted that the US military has bribed Iraqi journalists with under-the-table payoffs of up to $200 a month -- twice the average Iraqi monthly income -- for producing upbeat newspaper, radio and television reports about the war in Iraq. This follows a similar report yesterday that the military secretly paid Iraqi newspapers to run dozens of pro-American articles written by the US Information Operations Task Force in Baghdad. A Pentagon spokesman described the report as "troubling". "This article raises some questions as to whether or not some of the practices that are described in there are consistent with the principles of this department."
U.S. Govermnet Bribing Journalists
Administration Paid Commentator (WashPost membership rqd) The Education Department paid commentator Armstrong Williams $241,000 to help promote President Bush's No Child Left Behind law on the air, an arrangement that Williams acknowledged yesterday involved "bad judgment" on his part. I'm sure y'all check the Washington Post regularly, but isn't this simply bribing a journalist?
Microsoft Buys-off Foreign Governments
Microsoft Buys-off Foreign Governments. Can you say "Bribery?"
Cherry picking shopping
$20,000 bonus to official who agreed on nuke claim A former Energy Department intelligence chief who agreed with the White House claim that Iraq had reconstituted its defunct nuclear-arms program was awarded a total of $20,500 in bonuses during the build-up to the war, WorldNetDaily has learned...His officers argued at a pre-briefing at Energy headquarters that there was no hard evidence to support the alarming Iraq nuclear charge, and asked to join State Department's dissenting opinion, Energy officials say. Rider ordered them to "shut up and sit down," according to sources familiar with the meeting.
Worst Election Ever in PNG due to Mismanagement and Cash Crisis
Worst Election Ever in PNG due to Mismanagement and Cash Crisis
Papua New Guinea's two-week polling period has been extended one week in some areas due to a near complete breakdown of voting infrastructure: corrupt common rolls in some areas, violence in others, and no ballots or cash to pay for election services in still others. Results as they come in are available online. What went wrong? Outright bribery, sometimes. Its a fight over who will control massive natural resources: citizens or international conglomerates. The privatising PM may be heading for a fall, despite his mishandling of election.
Papua New Guinea's two-week polling period has been extended one week in some areas due to a near complete breakdown of voting infrastructure: corrupt common rolls in some areas, violence in others, and no ballots or cash to pay for election services in still others. Results as they come in are available online. What went wrong? Outright bribery, sometimes. Its a fight over who will control massive natural resources: citizens or international conglomerates. The privatising PM may be heading for a fall, despite his mishandling of election.
U.S. Representative James Traficant
U.S. Representative James Traficant (D-Ohio) has been found guilty on all 10 charges he faced, including kickbacks, fraud, bribery, and racketeering. The Congressman, known for his hatred of the IRS(God bless him) and his love of pork barrel projects, has lit up the Congress with his bombastic behavior since he was elected in 1984. Controversy has never been far from Traficant, he still claims that the trial is due to the bizarre, humorous, and grotesque story of his mob-funded election to Sheriff of Youngstown. He claims that he will run as an independent in the newly formed 17th District in the next congressional election. Will he be out of jail? Does he have a chance? What is Congress going to do with him? Fascinating background information courtesy of investigative journalist Dan Moldea's website
Cheney tried to collect $60 million for Enron on a recent trip to India...
Global Corruption Report 2001
Global Corruption Report 2001 - if you're not depressed already, this will make you so.
Tanzania 9th most corrupt country
Tanzania 9th most corrupt country, of course the word here is that they bribed transparency international to place them above kenya....... according to the director "HIV AIDS is killing millions of Africans, and in many of the countries where AIDS is at its deadliest the problem is compounded by the fact that corruption levels are seen to be very high. While it is imperative that richer countries provide the fruits of medical research at an affordable price to address this human tragedy, it is also essential that corrupt governments do not steal from their own people. This is now an urgent priority if lives are to be saved." local traditions don't help either. what this story does not say is that 4,000 girls will be circumsised at this ceremony and the govt/police won't interfere.
Philipines President Estrada impeached
Philipines President Estrada impeached The House has sent impeachment charges against the president to the Senate for trial, charging Estrada with taking millions of dollars in illegal payoffs. Mr Estrada has asserted that he has not received "a single centavo" from illegal gambling but he has acknowledged that he had been offered and refused a $4 million bribe.
Homeless given Cigarettes to vote for Gore
Homeless given Cigarettes to vote for Gore - Would they have wanted that kind of help if it wasn't caught on video tape? Maybe they should just steal the social security numbers of the dead and have THEM vote via mail-in. (See also: Zombies for Gore) [Links via Fark]
The Village Voice Endorses Ralph Nader for President
The Village Voice Endorses Ralph Nader for President This comes on the heels that Nader reportedly refuses to accept a bribe to drop out of the race.
Page: 1


text:
I would like to request for funds to complete my 3 weeks Training in HIV and AIDs. I have a balance o f Kenya Shillings 15,000($200) and I may not be given my certificate at the time of completion 27.02.09. I therefore forward this appeal so that I can be able to finish and leave with my certificate to enable me start offering my services to the community.
Thank You
Vincent Otieno Otuka
Hi! How has your training been so far? This sounds like an amazing experience and an incredible community tool! I have just a few questions that may help others contribute to your training. How much funding do you still need? What is the name of the organization that is conducting the training? Do they have a website? Would someone be able to wire the money directly to the organization?
A great place to post a request for resources on PulseWire is on the Resource ExChange page: http://www.worldpulse.com/pulsewire/exchange. Also, have you seen the Jambo, Hello from Kenya group (http://www.worldpulse.com/pulsewire/groups/1474)? Maybe a member in this group could direct you to organizations or donors that might be able to help you?
Best wishes,
BONJOUR LISA, I got your message and i was really wondering how i was going to do about it.One of my classmates helped me to settle my balance right now i was requesting perharps if somebody could give a helping hand(Fee) to persue a diploma course in hiv counselling & clinical set up at liverpool vct treatment & care.I will apreciate any assistance given to me. vincent otuka
Bonjour Amalia, I got your mail and i was unable to reply it due to the fact that i forgot my password.some time back i was requested for funds inorder to complete my education in the above intrest but the response i got i did not understant how to go about it pliz am just trying to imagine how you are going to help me and how does this make you feel? hope to hear from you as soon as possible A bientot


text:
Dentsu, Japanese advertising colossus, released a new Android client with D2 Communications and Bijutsu Shuppan Networks Co., Ltd.
The new client Decotter lets you tweet not only 140 characters text but also Deco Sozai(=decoration materials), colourful images and character letters.
FYI, those "deco moji" are not emoji, which is now part of Unicode.
With maximum 10 decorations, you may tweet up to 100 regular letters in a single tweet.
It's only available for Docomo and KDDI au. Not for Softbank Mobile.
Same as their successful "deco" business on their mobile web (i-mode/ezWeb), it co-works with a paid decoration selling service, Deco Market, where people can purchase more decorations to make their tweets stand out.
Dentsu's release [J, pdf]
See Also:
Povie – Purikura For Movie, Knows Your "Kawaii" Points To Decorate
Akky Akimoto
Latest posts by Akky Akimoto (see all)
- Smartphone Fasting Camp to be Held This Summer in Japan - May 25, 2016
- Japanese Web: Find Hilary Clinton's Photo Similar To Social Game Idols - March 7, 2016
- Yahoo! Japan to Open Silicon Valley Branch - March 7, 2016
- Yahoo! Japan and Family-mart Gives 50K Free Coffee For Search - February 16, 2016
- Google Japan's office in Skyscraper had a Small Fire - November 27, 2015


text:
2016-10-05 11:13
2016-09-16 16:36
2016-09-12 15:23
2016-09-08 11:03
2016-09-01 09:00
2016-08-26 08:00
2016-06-23 08:02
Looking for the ideal base oil for lubricants and greases? You're in the right place.
Looking for plastisizers for your formulation? Look no further.
A wide range of speciality oils for the print industry
Unbeatable solvency and good compatibility with SIS, SBS and TPV.
Can't find your application? Not to worry chances are we can still meet your needs.
A wide section of high-performance products for the toughest conditions.
We offer solutions for all climates and conditions. - plus on-site delivery world wide.
Solutions with great compatibility, both for polar and non-polar elastomers.
Decades of experience mean we are ready to meet your needs.
Speciality oil that increase quality and improve your tyre labeling grades.


text:
The supermodels are back in their homeland to get in on the festivities. Read More
Having a child changed how the Victoria's Secret Angel felt about modeling.Read More
The men's magazine may have put out their best Body Issue yet.Read More
The supermodel bares it all for the men's magazine. Read More
The model and actress looked stunning and slim at Michael Kors' Gold Collection launch in New York City in Sept. 2015, just two months after giving birth to her second son, Leo Thames.Read More
The 20-year-old model shared this Instagram shot after she and Kendall Jenner walked in their first Victoria's Secret Fashion Show on Nov. 10. "No one I would have rather shared my VS v-card with ;)," Hadid captioned the pic. "This show is genuinely a dream come true. Can't thank you enough @victoriassecret @ed_razek #vsfashionshow2015."Read More
The Victoria's Secret model has a mini me on her hands!Read More
Carson Daly, host of NBC's singing competition show The Voice, rocked the New Year's festivities and counted down to the start of 2014 in the middle of Times Square in New York City on December 31. (And then January 1 immediately after.)Read More
These angels are ready to "shake it off" and hit the runway!Read More
Forty-seven Victoria's Secret Angels headed to London for the VS Fashion Show and ET's Kevin Frazier flew in style with the models.Read More
These Brazilian beauties weren't the lucky charms Brazil thought they were.Read More
Here are some tips from stylish celebs on how to celebrate the stars and stripes in style.Read More
Here's a look at some celebrities making waves on Instagram and flaunting their fab physiques!Read More


text:
Life Changing Holidays ; sort out your problems on an award winning Counseling Retreat, Life Coaching Holiday or Couples Therapy Retreat.
Stressful situations and difficult feelings?
Unhappy, tired, stuck...unsure what to do next...
Relax in tropical sunshine in Thailand and sort out your problems. We have condensed the best evidence based approaches and offer a Counseling Retreat and Life Coaching Holiday both built around a highly successful 16 hour program with 12 weeks follow up support.
Relationship problems?
We also run special Couples Therapy Retreats.
What's more, we offer these therapy retreats as part of a tailor made holiday in Thailand, so that you can get away, relax in the sun, think more clearly and sort out your problems all in one go.
Impossible dilemmas?
We'll help you overcome whatever difficulties life is throwing at you.
We'll increase your confidence, clarify your decision making and help you establish new ways forward. Your Therapy Retreat will provide you with a clear Change Plan, plus the skills and support you need to make it happen.
Award winning life changing retreats by dedicated professionals that really work
"Better than I dared hope. It was like taking a vacuum cleaner to the dust in my brain" — Esquire Magazine
Life Coaching Holidays
Relax by the pool in your 5* boutique hotel, sip a cocktail and watch butterflies flit through the orchids. You feel relaxed and your mind is clear for the first time in ages. This is no ordinary Life Coaching Holiday... these are true Life Changing Holidays.FIND OUT MORE
Couples Therapy Retreat
Your partner is learning how to fly a plane. You have just had a massage. You both feel good and relaxed. What's more, you are both speaking together for the first time in ages. This isn't any old relationship counseling,this is an award winning Therapy Retreat for Couples.FIND OUT MORE
Counseling Retreat
Hi, this is a 1 or 2 week counseling retreat holiday for individuals. Our life changing retreats allow you to sort out your problems, overcome difficulties and find news ways forward while you relax in 5 star tropical luxury in Thailand. Whatever life is throwing at you we are ready to help. We have helped 100's of people over the past 20 years and are looking forward to being able to help you too.This is no ordinary holiday this is an award winning Life Changing Holiday in Thailand.FIND OUT MORE
Life Change Blog: Latest Posts
Loving Your Partner Through Mental Illness
By Tara Bosler – freelance writer on home, kids, family, & love Although as a society we are becoming more aware of mental health issues, there is still a significant stigma attached to individuals that suffer from mental illness. There is a lot that the general population does not know or understand about mental illness […]READ MORE
A Motivational Approach to Relationship Counselling
Why do any of us change? This is the most important question that lies at the heart of all counselling. It's all about change. Relationship Counselling often gets side tracked by all kinds of stuff; blaming each other, trawling over the past, outlining past broken promises, explaining behaviours, threatening each other to name but a […]READ MORE
Coping With Financial Loss
12 Life Coaching Techniques to Deal With Financial Anxiety Grief, anxiety and depression, as a result of financial loss are as real and damaging as the loss of a loved friend or close relative. In some cases it's even worse. There can't be many other stories that illustrate this point more shockingly and dramatically than […]READ MORE


text:
Search results
- www.skyjim.com CachedSBOBET Games - Play online slots poker lottery virtual racing and casino games for FREE or with real money to enjoy generous payouts.
- info.skyjim.com CachedSBOBET Information Center - Help | FAQ | Tutorials | Glossary - Visit SBOBET Info Center for help and assistance.
- www.skyjim.com/en/legal/terms-and-
conditions/b2b Cached2.1 The Betting Platform Provider reserves the right to amend these terms and conditions (including to rectify errors or mistakes discovered on the Betting Platform ...
- info.skyjim.com/glossary CachedTerm Description; 1X2: A bet type in SBOBET Sports. Bets can be made by either selecting a Home Win a Draw or an Away Win. Bets will be settled at the end of normal ...
- info.skyjim.com/category/7/0/10 CachedSBOBET Help - FAQ - Find answers to all your questions about your SBOBET account. Learn about opening activating and managing your SBOBET account.
- info.skyjim.com/article/AA-00270/0/Who-
is-SBOBET.html CachedSBOBET is one of the world's leading online gaming brands. Winner of the Asian Operator of the Year Award for the year 2009 and 2010 with operations in Asia ...
- info.skyjim.com/article/AA-00679/0/How-
do-I-use-the-bet...SBOBET Help - FAQ - Read tutorial to learn more about the SBOBET bet slip and how you can use it to make bet selections and place bets in Financials.
- info.skyjim.com/article/AA-00678/0/How-
do-I-place-a-bet... CachedSBOBET Financials is a binary options betting platform that is easy to operate. You will find trading binary option is so effortless simply by following the steps as ...
- info.skyjim.com/article/AA-00124/0/What-
can-I-use-to... CachedSBOBET Help - FAQ - Find out the payment methods you can use to deposit funds into your SBOBET account.
- www.skyjim.com/en/games/web-root/
flexible/redirect... Cachedwww.skyjim.com


text:
Histone 3 Lysine 9 Trimethylation Is Differentially Associated With Isocitrate Dehydrogenase Mutations in Oligodendrogliomas and High-Grade Astrocytomas
Trimethylation of histone 3 lysine 9 (H3K9me3) is a marker of repressed transcription. Cells transfected with mutant isocitrate dehydrogenase (IDH) show increased methylation of histone lysine residues, including H3K9me3, because of inhibition of histone demethylases by 2-hydroxyglutarate. Here, we evaluated H3K9me3 and its association with IDH mutations in 284 gliomas. Trimethylation of H3K9 was significantly associated with IDH mutations in oligo-dendrogliomas. Moreover, 72% of World Health Organization grade II and 65% of grade III oligodendrogliomas showed combined H3K9me3 positivity and 1p19q codeletion. In astrocytic tumors, H3K9me3 positivity was found in all grades of tumors; it showed a significant relationship with IDH mutational status in grade II astrocytomas but not in grade III astrocytomas or glioblastomas. Finally, H3K9me3-positive grade II oligodendrogliomas, but not other tumor subtypes, showed improved overall survival compared with H3K9me3-negative cases. These results suggest that repressive trimethylation of H3K9 in gliomas may occur in a context-dependent manner and is associated with IDH mutations in oligodendrogliomas but may be differently regulated in high-grade astrocytic tumors. Furthermore, H3K9me3 may define a subset of grade II oligodendrogliomas with better overall survival. Our results suggest variable roles for IDH mutations in the pathogenesis of oligo-dendrogliomas versus astrocytic tumors.
- Astrocytoma
- Glioma
- H3K9me3
- Histone methylation
- Isocitrate dehydrogenase
- Oligodendroglioma
Epigenetic mechanisms such as DNA methylation and histone methylation play a significant role in the pathogenesis and progression of tumors. DNA methylation has been extensively studied in glioblastomas (1–3). In contrast, histone modifications in glioma pathogenesis have remained poorly understood. Recent studies have emerged implicating histone modifications in adult gliomas (4–6) and histone gene mutations in pediatric glioblastomas (7, 8). Histones are proteins that form octamers consisting of H2A, H2B, H3, and H4 around which DNA is wrapped. Histones have amino acid tails that are subject to various modifications such as acetylation, methylation, phosphorylation, ubiquitination, and SUMOy-lation of arginine (R) and lysine (K) residues, resulting in changes in DNA function and transcription (9). Histone lysine methylation can be an activator or suppressor of transcription, depending on the histone residue that is methylated. For example, methylation of H3K4, H3K36, and H3K79 is associated with activation of transcription, whereas methylation of H3K9, H3K27, and H4K20 is associated with silencing of transcription (9, 10).
Histone lysine methylation is regulated by histone lysine methyltransferases (KMTs) and demethylases (KMDs). One of the earliest KMTs to be characterized was SUV39H. SUV39H belongs to the SET domain family and specifically methylates H3K9 (11). Subsequently, H3K9 may be the most extensively studied site of histone methylation (12). Trimethylation of H3K9 (H3K9me3) is associated with pericentric heterochromatin formation, transcriptional repression, and DNA methylation (13–15).
Very little is known about H3K9 modifications in glioma pathogenesis. Trimethylation of H3K9 is increased in cells expressing mutant isocitrate dehydrogenase 1 (IDH1 R132H) (4, 5, 16). The NADP-positive dependent enzyme IDH is mutated in approximately 70% of World Health Organization (WHO) grade II and grade III gliomas and secondary glioblastomas (17–19). Mutations in IDH result in neomorphic activity of the enzyme catalyzing the production of the oncometabolite 2-hydroxyglutarate (2-HG) from α-ketoglutarate (α-KG) (20, 21). Oncometabolite 2-HG is a structural analog of α-KG and can inhibit several α-KG-dependent oxygen-ases (22, 23). The α-KG-dependent oxygenases form a large family of enzymes that influence various cellular functions, such as collagen modifications, carnitine synthesis, hypoxic sensing, and histone and DNA demethylation (24). For example, KDM4C (belonging to the Jumonji C family of KDMs) uses α-KG, oxygen, and Fe(II) as cofactors to demethylate histone lysine residues. Immortalized human astrocytes or murine neurosphere cultures overexpressing IDH1 R132H show increases in H3K9me3 compared with cells overexpressing wild-type IDH1 (4, 16)l, which is mediated in part by the inhibition of KDM4C by 2-HG (4). Similarly, heterozygous knock-in of IDH1 R132H in HCT116 colon cancer cell lines results in increased H3K9me3 (5).
Based on these observations, we hypothesized that IDH mutant gliomas would show increased H3K9me3. The goals of this study were to evaluate the relationship between H3K9me3 and IDH mutations in gliomas of different subtypes and grades and to determine if H3K9me3 had any prognostic significance in gliomas. The results suggest that the effect of IDH mutations on histone methylation may differ between oligodendrogliomas and high-grade astrocytomas and may provide insight into the role played by mutant IDH in the pathogenesis of these tumors.
Materials and Methods
Case Material
Cases were obtained from the University of California San Francisco, the University of Pennsylvania, the University of Pittsburgh, Children's Hospital of Philadelphia, and Memorial Sloan-Kettering Cancer Center (MSKCC) after approval from the respective institutional review boards. All cases were deidentified before analysis and included both whole tissue and tissue microarray sections (Supplemental Digital Content 1, Methods, http://www.jnen.oxfordjournals.org/lookup/suppl/doi:10.1097/NEN.0b013e3182898113/-/DC1).
The cohort demographics consisted of a total of 284 gliomas (Table 1). The tumors consisted of 263 adult gliomas (53 WHO grade II oligodendrogliomas, 47 grade III anaplastic oligodendrogliomas, 6 grade I pilocytic astrocytomas [supratentorial], 28 grade II diffuse astrocytomas, 43 grade III anaplastic astrocytomas, and 86 grade IV glioblastomas) and 21 grade I pediatric pilocytic astrocytomas (14 cerebellar and 8 supratentorial). Each case was reviewed individually by a neuropathologist at each institution and then confirmed by a neuropathologist at MSKCC. Loss of heterozygosity (LOH) data for 1p and 19q loci was available in 51 of 53 of grade II and 40 of 47 grade III oligodendrogliomas. In oligodendrogliomas, 84% (43 of 51) of grade II and 75% (30 of 40) of grade III contained codeletions of chromosomal arms 1p and 19q.
|Age, years
|IDH Mutant
|Tumor Type and WHO Grade
|Total Positive, n (%)
|Total Positive, n (%)
|Oligodendroglioma II
|44 (83)
|46 (87)
|Oligodendroglioma III
|37 (79)
|41 (87)
|Pilocytic astrocytoma I
|13 (48)
|0 (0)
|Diffuse astrocytoma II
|22 (78)
|20 (71)
|Astrocytoma III
|29 (67)
|24 (56)
|Primary glioblastoma IV
|55 (78)
|0 (0)
|Secondary glioblastoma IV
|13 (81)
|16 (100)
F, female; H3K9me3, trimethylation of histone 3 lysine 9; IDH, isocitrate dehydrogenase; M, male.
Immunohistochemistry and Scoring
Immunohistochemical studies were performed as previously published (4, 25, 26). In brief, immunohistochemical detection was performed using the Discovery XT processor (Ventana Medical Systems, Tucson, AZ). Tissue sections were blocked for 30 minutes in 10% normal goat serum in 2% bovine serum albumin in PBS. Sections were incubated for 5 hours with 0.1 µg/mL of the rabbit polyclonal anti-H3K9me3 (ab8898; Abcam, Cambridge, MA) or mouse monoclonal anti-IDH1 R132H (1:30, DIA H09; Dianova, Hamburg, Germany) antibodies. Tissue sections were then incubated for 60 minutes with biotinylated goat anti-rabbit IgG (PK6101; Vector Laboratories, Burlingame, CA) or goat anti-mouse IgG (BA9200; Vector Laboratories) at 1:200 dilution. Blocker D, streptavidin-horseradish peroxidase, and 3,3′-diaminobenzidine detection kit (Ventana Medical Systems) were used according to the manufacturer instructions.
Whole-mount mouse embryos were used as positive controls for H3K9me3 (27). Trimethylation of H3K9 was also assessed in normal cortical adult postmortem brain tissue obtained from 4 cases. Trimethylation of H3K9 in all of those cases was negative in glia and in endothelial cells but showed scattered nuclear staining in neurons (Figure, part A, Supplemental Digital Content 2, http://www.jnen.oxfordjournals.org/lookup/suppl/doi:10.1097/NEN.0b013e3182898113/-/DC1). Two individuals scored H3K9me3 and IDH1 R132H as positive or negative in a blinded manner. Only nuclear staining for H3K9me3 in tumor cells was considered positive. Preservation of antigenicity in tumor sections that were negative for H3K9me3 was confirmed by retained neuronal nuclear staining in surrounding brain tissues as an internal control when available for evaluation (Figure, part C, Supplemental Digital Content 2, http://www.jnen.oxfordjournals.org/lookup/suppl/doi:10.1097/NEN.0b013e3182898113/-/DC1). Sections stained for IDH1 R132H were scored positive or negative based on described criteria (25, 28, 29).
IDH1/2 Mutational Analyses
Genomic DNA was extracted from formalin-fixed paraffin-embedded blocks from two 10-µm-thick slices using the Formapure kit (Agencourt, Beverly, MA) in a 96-well format, using a modified version of the manufacturer's method and in a semiautomated fashion. Isocitrate dehydrogenase 1/2 mutational analyses were performed on cases that were IDH1 R132H negative using previously described protocols (26, 30). Because of limitations of tissue availability, cases from the tissue microarray containing glioblastomas and pilocytic astrocytomas were not available for mutational analyses.
Statistical Analysis
Statistical analyses were performed in consultation with the MSKCC biostatistics core. SPSS (version 21; IBM, Chicago, IL) and Prism (version 5; Graphpad, La Jolla, CA) were used to analyze data. Fisher exact test was used to determine the odds ratios to evaluate the strength of association between IDH mutational status and H3K9me3 positivity or the association between 1p-19qdeletion and H3K9me3 positivity in oligoden-drogliomas. The log-rank (Mantel-Cox) test was used to examine the association of H3K9me3 positivity on overall survival within each tumor subtype. Multivariate survival analysis was performed using the Cox proportional hazards model. Differences were considered significant when p < 0.05 (95% confidence intervals).
H3K9me3 Is Associated With IDH Mutations in Oligodendrogliomas
Positive H3K9me3 nuclear staining in gliomas was seen in 83% (44 of 53) of grade II oligodendrogliomas and 79% (37 of 47) of grade III oligodendrogliomas (Table 1; Fig. 1). There was a significant relationship between H3K9me3 positivity and IDH mutations in grade II oligodendrogliomas (76% [40 of 53] of cases with IDH mutations and positive for H3K9me3; odds ratio, 8.9; p = 0.01) and grade III oligodendrogliomas (75% [35 of 47] of cases with IDH mutations and positive for H3K9me3; odds ratio, 11.7; p = 0.01) (Figs. 1, 2).
H3K9me3 in Astrocytic Tumors
Positivity of H3K9me3 was as follows in astrocytic gliomas: 48% (13 of 27) of pilocytic astrocytomas (Figure, part B, Supplemental Digital Content 2, http://www.jnen.oxfordjournals.org/lookup/suppl/doi:10.1097/NEN.0b013e3182898113/-/DC1), 78% (22 of 28) of diffuse astrocytomas, 67% (29 of 43) of anaplastic astrocytomas, and 79% of glioblastomas (68 of 86) (Table 1; Figs. 2, 3). As expected, all pilocytic astrocytomas were IDH1 R132H negative. Trimethylation of H3K9 showed a significant association with IDH mutations in diffuse astro-cytomas (65% [18 of 28] of cases with IDH mutations and positive for H3K9me3; odds ratio, 9; p = 0.04; Figs. 2, 3). No significant relationship between IDH mutations and H3K9me3 positivity was observed in anaplastic astrocytomas or glioblastomas (Figs. 2, 3).
H3K9me3 Is More Frequent but Not Significantly Associated With LOH of Chromosomes 1p and 19q in Oligodendrogliomas
Loss of heterozygosity information for 1p-19q was available in 46 of 53 of grade II and 40 of 47 grade III oligodendrogliomas (Table, Supplemental Digital Content 3, http://www.jnen.oxfordjournals.org/lookup/suppl/doi:10.1097/NEN.0b013e3182898113/-/DC1). Codeletions 1p-19q showed a strong correlation with IDH mutational status (grade II oligodendrogliomas: odds ratio, 41; p = 0.0001; and grade III oligodendrogliomas: odds ratio, 28.5; p = 0.01). Two grade II oligodendrogliomas had codeletions but did not bear IDH mutations. All grade III anaplastic oligodendrogliomas with codeletions bore IDH mutations. Positivity of H3K9me3 and 1p-19q codeletions were noted in 72% (37 of 51) of grade II and 65% (26 of 40) grade III oligodendrogliomas. Trimethylation of H3K9 was positive in 12% (6 of 51) of grade II and 17% (7 of 40) of grade III oligodendrogliomas with intact 1p-19q. Trimethylation of H3K9 was negative in 14% (7 of 51) of grade II and 10% (4 of 40) of grade III oligodendrogliomas with 1p-19q codeletions (Fig. 4; Table, Supplemental Digital Content 3, http://www.jnen.oxfordjournals.org/lookup/suppl/doi:10.1097/NEN.0b013e3182898113/-/DC1). However, Fisher exact test did not reveal a statistically significant relationship between H3K9me3 and loss of 1p-19q in either grade II (odds ratio, 0.8; p = 1) or grade III (odds ratio, 2.8; p = 0.3) oligodendrogliomas (Table, Supplemental Digital Content 4, http://www.jnen.oxfordjournals.org/lookup/suppl/doi:10.1097/NEN.0b013e3182898113/-/DC1).
H3K9me3 Is Associated With Improved Overall Survival in Grade II Oligodendrogliomas
Overall survival was improved in H3K9me3-positive grade II oligodendrogliomas versus their H3K9me3-negative counterparts (log-rank [Mantel-Cox] test, hazards ratio, 5.9; p = 0.005) (Fig. 5A; Table 2). Multivariate analysis using the Cox proportional hazards model showed that H3K9me3 positivity was a significant prognostic factor (n = 53, p = 0.015), but age, sex, or IDH mutational status were not (Table, Supplemental Digital Content 4, http://www.jnen.oxfordjournals.org/lookup/suppl/doi:10.1097/NEN.0b013e3182898113/-/DC1). 1p-19q LOH showed a trend toward improved overall survival (p = 0.052) (Table, Supplemental Digital Content 4, http://www.jnen.oxfordjournals.org/lookup/suppl/doi:10.1097/NEN.0b013e3182898113/-/DC1). No significant difference in survival was noted in grade III oligodendrogliomas (log-rank [Mantel-Cox] test, hazards ratio, 1.6; p = 0.4; Fig. 5B; Table 2). No significant associations were observed in overall survival between H3K9me3-positive and -negative gliomas, regardless of grade in astrocytic tumors (Table 2).
|Median Survival, days
|Log-Rank Test
|Tumor Type and WHO Grade
|H3K9me3 Positive
|H3K9me3 Negative
|H3K9me3 Positive
|H3K9me3 Negative
|Oligodendroglioma II
|Oligodendroglioma III
|Astrocytoma II
|Astrocytoma III
|Glioblastoma IV
Patients were stratified according to H3K9me3 positivity (n indicates number of H3K9me3 positive or negative cases for each tumor subtype). The log-rank (Mantel-Cox) test with 95% confidence intervals was used to determine differences in overall survival.
↵* Percent survival in this group at the end of the study was greater than 50% and was thus undefined by the statistical software.
Isocitrate dehydrogenase mutations are seen in approximately 70% of intermediate-grade gliomas. How mutations in IDH contribute to brain tumor development is not known. Mutant IDH catalyzes the production of 2-HG from α-KG (20, 21). Oncometabolite 2-HG is structurally similar to α-KG and inhibits α-KG-dependent dioxygenases, such as the Jumonji C family of histone demethylases, resulting in increased histone methylation marks, including H3K9me3 in vitro (4, 22, 23). We sought to determine the relationship between H3K9me3 and IDH mutations in gliomas. We found significant associations between the 2 factors in oligodendrogliomas and grade II astrocytomas. Whereas 67% of anaplastic astrocytomas and 78% of glioblastomas showed H3K9me3 positivity, there was no significant association with IDH mutations. Finally, H3K9me3 showed a beneficial survival effect in grade II oligodendrogliomas but not in grade III oligodendrogliomas or in any grade of astrocytic tumors.
Because IDH mutations are closely associated with 1p-19q codeletions (18, 31–33), we considered whether H3K9me3 was associated with 1p-19q deletions in oligodendrogliomas. Indeed, 72% of grade II and 65% of grade III oligodendrogliomas that contained 1p-19q deletions were H3K9me3 positive. This association was not, however, statistically significant, suggesting that 1p-19q deletions may not directly regulate histone methylation. Recurrent mutations in the CIC gene (homolog of the Drosophila gene capicua) on chromosome 19q and FUBP1 gene (FUSE binding protein 1) on chromosome 1p have been recently described in oligodendrogliomas (34, 35). These proteins are thought to influence the RAS/MAP-kinase pathway (CIC) and MYC activation (FUBP1) and thus may not directly methylate histones (34).
Grade II oligodendrogliomas that are H3K9me3 positive, but not other glioma subtypes, showed significantly improved overall survival. Future studies with a larger cohort are needed to confirm this observation, but the prognostic significance of H3K9me3 needs to be interpreted with caution. Although H3K9me3 showed a significant relation with IDH mutations in oligodendrogliomas and grade II astrocytomas, we did not observe a one-to-one correspondence. For example, 7% of diffuse astrocytomas, 11% of grade II oligo-dendrogliomas, and 13% of anaplastic oligodendrogliomas were IDH mutant but H3K9me3 negative. Conversely, 14% of diffuse astrocytomas, 5% of grade II oligodendrogliomas, and 4% of anaplastic oligodendrogliomas were H3K9me3 positive but IDH wild type. We thus did not expect the prognostic value of IDH mutations to entirely translate to H3K9me3. One of the limitations of this study is that we are restricted to assessing H3K9me3 by immunohistochemistry because of tissue availability. Nevertheless, these observations reflect the complexity of the pathogenesis of IDH mutant gliomas. Multiple mechanisms downstream of mutant IDH and 2-HG may contribute to the pathogenesis with H3K9me3, representing one piece of the puzzle (36). Nevertheless, the finding that trimethylation of H3K9 is differentially regulated in oligodendrogliomas and high-grade astrocytomas is a significant observation. It suggests that mutant IDH may have different roles in oligodendrogliomas versus high-grade astrocytomas; thus, it may provide clues for understanding the pathogenesis of these tumors.
We found significant H3K9me3 positivity in anaplastic astrocytomas and glioblastomas without any significant associations with IDH mutations. Similarly, H3K9me3 exhibited no prognostic associations in these tumors. Methylation of H3K9 is regulated by a complex dynamic interplay involving the enzymatic activity of KMTs and KDMs. It is possible that the activity of these enzymes is deregulated in higher grade astro-cytomas and glioblastomas by means of a yet undefined process that is independent of the effect of mutant IDH. Furthermore, DNA methylation can promote histone methylation and vice versa. Isocitrate dehydrogenase mutations result in DNA methylation and reprograming the methylome to the glioma-CpG island methylation phenotype (G-CIMP), which could in turn influence H3K9 methylation (16). DNA methyltransferases can also influence histone methylation (37–39). Because Dnmt1 and Dnmt3 are deregulated in glioblastomas (40, 41), it is possible that aberrant activity of these enzymes mediates trimethylation of H3K9 in an IDH mutant/2-HG-independent manner. The data suggest the hypothesis that H3K9 can be trimethylated in high-grade astrocytomas and glioblastomas in both IDH1 mutant/2-HG-dependent and -independent manner. Furthermore, other epigenetic marks such as H3K27me3 may be altered in these tumors. Future experiments examining this question will address this issue.
A high frequency of positivity of H3K9me3 was observed in all subtypes or grades of gliomas including pilocytic astrocytomas. Pilocytic astrocytomas show alterations in BRAF (42), which is known to alter the methylome in melanomas and colon cancer (43, 44). Furthermore, melanomas bearing the BRAF(V600E) mutation show amplification of SETDB1, a H3K9 KMT resulting in increased H3K9me3 (45). It is possible that BRAF alterations contribute to H3K9me3 in pilocytic astrocytomas. To date, few studies have examined H3K9 modifications in glioma pathogenesis or glial development. Trimethylation of H3K9 is thought to play a role in differentiation (46–48). Neurosphere cultures overexpressing mutant IDH1 R132H show increased H3K9me3 accompanied by a decrease in glial fibrillary acidic protein expression compared with cells overexpressing wild-type IDH1, suggesting that H3K9me3 may contribute to repressing glial differentiation in IDH mutant gliomas (4).
Histone methylation remains poorly understood in gliomas and may be vital to further our understanding of the pathology of gliomas. We hypothesize that repressive trimethylation of H3K9 can occur in gliomas in IDH mutant-dependent or -independent fashion. Repressive H3K9 trimethylation may be regulated differently in oligodendroglial and astrocytic tumors and may be associated with IDH mutations in oligodendro-gliomas but may be regulated by other, yet undefined, mechanisms in astrocytic tumors. This difference in H3K9 trimethylation could represent one of the ways by which IDH mutations play different roles in oligodendrogliomas and astrocytomas. Moreover, IDH mutations show differential associations with other genetic alterations in oligodendrogliomas versus astrocytomas. Isocitrate dehydrogenase mutations in oligodendrogliomas are associated with 1p19q codeletions; whereas in astrocytic tumors, IDH mutations are closely associated with ATRX and TP53 mutations (8, 49–52). ATRX is an SWI/SNF chromatin re-modeler and plays a role in deposition of histone variant H3.3 (53). How IDH mutations, histone methylation, and ATRX mutations contribute to the pathogenesis of gliomas is not known. The interplay between these complex genetic alterations and histone methylation in oligodendrogliomas and astrocytomas is an avenue for future experimentation.
Supporting Information
The authors thank the MSKCC cytology core facility, the Geoffrey Bene Translational core, and Jianan Zhang for expert technical assistance and Elyn Riedel from the MSKCC biostatistics core for help with data analysis. The authors also thank Dennis Pozega, Patrick Ward, and Tullia Lindsten for critical reading of the manuscript.
This work was supported in part by grants from the NCI and NIH to Craig B. Thompson. Craig B. Thompson is cofounder of Agios Pharmaceuticals and has financial interests in Agios. The authors of this study declare no other potential conflicts of interest.
Supplemental digital content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal's Web site (http://jnen.oxfordjournals.org/).
- © 2013, by the American Association of Neuropathologists, Inc.


text:
Subscribe / Renew
Contact Us
Construction Bids
|Showing result(s) 101 - 120 of 457
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|Compusave, Inc.
|Lynnwood, WA
|Computer hardware, software and periferals IBM, HP System and server integration and maintenance Wired and wireless networking On-site and in-house...
|Computer NET Services
|Federal Way, WA
|Computers support new, used, Upgraded and repair. Networks Email Web Services include design and hosting. A complete computer service company.
|Concept Engineering
|Issaquah, WA
|Practice mainly involves all aspects of real property development-- including commercial, single family and multifamily projects. We also offer servic...
|Conestoga-Rovers & Associates
|Tacoma, WA
|Worldwide engineering, Environmental, Construction and IT Services
|Construction Defect Consulting Inc.
|Seattle, WA
|With over three decades of experience in construction industry and completion of over 400 projects, Construction Defect Consulting Inc. (CDC) is an in...
|Control Technology Inc.
|Kirkland, WA
|Control Technology has been in business since 1993. Our firm is a proven provider of engineered electrical and control system solutions to the indu...
|Copper Parrot Studios
|Edmonds, WA
|Architectural Visualization services for residential, commercial, and institutional projects. Copper Parrot Studios provides basic models for plan...
|Core Design, Inc.
|Bellevue, WA
|Core Design, Inc. provides Civil Engineering, Planning, Surveying, Landscape Architecture and Urban Design services to the Puget Sound commercial, res...
|Cornerstone Concilium Inc.
|Seattle, WA
|Cornerstone Concilium, Inc. is a consulting firm providing specialized services in the facilities, transporation, and technology industries. We have ...
|Cosmopolitan Engineering Group Inc.
|Tacoma, WA
|Cosmopolitan Engineering Group, Inc. is a certified MBE professional services corporation providing comprehensive consulting civil engineering, enviro...
|Cotey Consulting Services, Inc.
|Olympia, WA
|CCS services are grouped under Information Technology Management Consulting and Information Systems Delivery and Maintenance. Cotey Consulting Service...
|Cushing Associates, Civil Engineers
|Bremerton, WA
|Civil Engineering firm supporting traditional civil engineering projects and also experience in telecommunication tower projects.
|Cushing Associates, Civli Engineers
|Portland, OR
|Civil engineering since 1997, civil engineering, planning, CAD drafting and services, graphic design, website design, telecommunications projects are ...
|Custom Database Solutions
|Woodinville, WA
|Custom database design services and support for the General Contractor, Architect/Engineer and Subcontractor. Generic project document computer servic...
|D Hittle & Associates Inc.
|Lynnwood, WA
|This is the Puget Sound office. The headquarters are located in Kennewick.
|D Hittle & Associates Inc.
|Kennewick, WA
|D Hittle & Associates Inc. is now located in Kennewick. Electrical Engineers and Consultants providing engineering services for publicly and cooperati...
|Dames & Moore
|Portland, OR
|Environmental & engineering consulting services
|Davis & Davis Interior Design Build Firm
|Kennewick, WA
|A Trend Setting, Modern, Cutting Edge Commercial Design & High End Residential Design /Build Firm. Specializing in Show & Model Homes, Out of the B...
|DBM Consulting Engineers
|Auburn, WA
|DBM offers full service infrastructure services including civil engineering, land planning, surveying, environmental, construction management and inve...
|Fircrest, WA
|I offer complete Construction Drafting services from the initial design phase all the way through the Permit process. Whether its a Residential or Com...
|Showing result(s) 101 - 120 of 457
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |