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You've marked your calendar for October 27.
You've read the reasons you should watch the biggest event in online chess history.
You've read the stats and analytics showing just how incredibly accurate the two combatants pl... | Read More
One of the most exciting projects I've contributed to since joining the Chess.com team (eight years ago and counting) is being revealed today.
Chess.com's Computer Accuracy & Precision Score (or CAPS) is a... | Read More
Attention all chess fans: This is not a drill.
This Thursday, Magnus Carlsen faces Hikaru Nakamura for the inaugural Chess.com Grandmaster Blitz Battle Championship.
It's the match we've all been waiting for, and you can watch it live next Thu... | Read More
Charles de Maurian, well known as Paul Morphy's childhood and life-long friend, developed very quickly as a chess player. He began learning the game from Morphy during the 1853-4 school year at Spring Hill College ... | Read More
Editor's note: Hikaru Nakamura is widely considered the best speed chess player in history. A vote by titled players confirmed this last year.
Nakamura will face World Champion Magnus Carlsen in the three-hour GM Blitz Ba... | Read More
by HJS
When God asked Abraham to give his son as a sacrifice on Mount Moriah, he complied without questioning God as to why He asked this most unusual thing, especially when the boy had been a gift from God. As Abraham approached Moriah, he turne... | Read More
The Game of the CenturyThis week marked 60 years which is considered one of the best games of the twentieth century, the brilliant victory of the young American talent Robert James "Bobby" Fischer GM against Donald Byrne in the Rosenwald tournamen... | Read More
Brothers Alan and Neal Evans, on drums and Hammond B-3 organ, respectively, form two-thirds of the souljazz/groove trio Soulive. Rounding out the group is Eric Krasno on guitar. The band was formed in the late '90s when all three members ... | Read More
by HJS
From the beginning of recorded history men and women have become entrapped by habits that lead to addictions.
An addiction is a thirst that, if denied, has devastating consequences. When you are addicted and denied the drugs, work, food, ... | Read More
They called it the game of the century. It was not football, tennis or any of the other sports that normally draw millions of viewers and attract multimillion-dollar sponsors. This was a battle of wits between a 29-year-old chess master Donald B... | Read More
GMs Alexander Riazantsev and Alexandra Kosteniuk are the winners of this year's Russian Championship Superfinal. Kosteniuk claimed the women's title with a round to spare; Riazantsev decided matters today. Both won a Renault Ka... | Read More
In a Superfinal that continues to see a huge number of draws, the 31-year-old grandmaster, Alexander Riazentsev, ended the Superfinals deadlock in round eight. Meanwhile, Alexandra Kosteniuk continues to dominate the women's tournament. ... | Read More
In boxing there was Ali vs Frazier; in tennis, Navratilova against Evert.
In online chess there is Carlsen vs Nakamura.
Although World Champion GM Magnus Carlsen and four-time U.S. champion GM Hikaru Nakamura have not rumbl... | Read More
It's anyone's guess who will win the Russian Championship's Superfinal where, near the half-way point, six players are tied for first place. Today, Alexander Grischuk, Dmitry Jakovenko, and Evgeny Tomashevsky all joined the leaders.... | Read More
The Russian Championship's Superfinal has started slowly—14 of 18 games have been drawn. However, there's been plenty of bloodshed in the women's section. They've had 13 decisive games!
Three rounds have been played at the Superfinal, the ... | Read More
Can you get any more excited for the biggest chess event in online history? It's Magnus Carlsen vs Hikaru Nakamura in the Chess.com GM Blitz Battle Championship, live on Oct. 27. Check out this brand new promo video to get even more hyped. | Watch video
Magnus Carlsen is ready to play the strongest online blitz match in chess history on Tuesday as he faces a final four with Alexander Grischuk, Hikaru Nakamura, and Maxime Vachier-Lagrave. Find out who the ChessCenter team picks to win each match.
Did chess play a role in Michael Vick's redemption story? Learn how the game can teach more than tactics and strategy.
Plus, get the wrap-up from Wesley So's big win at the Sinquefield cup, all on this week's ChessCenter. | Watch video
FM Mike Klein was on the scene for the 2016 U.S. chess championships, so he is the perfect analyst to bring you the most historic moments from the big event.
Sit back and enjoy these 11 bits of chess history as you relive the excitement from St. Louis. | Watch video
Who were the best chess players ever, and when were they dominant? ChessCenter takes a look at a beautiful video to answer those questions.
Enjoy a recap of the Grischuk-Aronian Blitz Battle, a US Chess Champs preview, and all the other news in the chess world. | Watch video
IM Danny Rensch and IM Lawrence Trent perform double duty in the 1st part of this double death match as they not only play the game but also talk all the trash! With perhaps the most contested finish in DeathMatch History you'll want to stick around until the end! | Watch video
Shogi is the Japanese form of Chess. It has been evolving in a different direction as international Chess for more than 1000 years, however, and its history contains many Shogi variants, some even still played today. Modern Shogi is played on a 9x...
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Arthur W. Dake (1910-2000) became a bridge toll collector, then a highway auto controller, and finally an automobile inspector for the state of Oregon after serving in the merchant marines when he was 16. He learned how to play chess at age 17 ...
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Maxime Vachier-Lagrave, born October 21, 1990, is a French Grandmaster. In 2002, he was 2nd in the World Under-14 Championship. In 2005, he became a Grandmaster at the age of 14 years, 4 months, the 7th youngest GM in history and the 2nd young...
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Baron Tassilo von Heydebrand und der Lasa (von der Lasa) was born on October 17, 1818 in Berlin, Germany. He studied law in Berlin and Bonn, Germany.
In 1837, he was one of the seven co-founders of the Berlin Chess School (the Berlin Pleiades),...
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The Evans Gambit is characterised by the moves:
1. e4 e52. Nf3 Nc63. Bc4 Bc54. b4
The gambit is named after the Welsh sea Captain William Davies Evans, the first player known to have employed it. The first game with the open...
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Chess:A History
by dasraj130702 4 months ago
The 10 Most Important Moments in Chess History (chess home page)
by SARA_SA 2 years ago
How important is chess history to you
by maDawson 3 years ago
A History of Chess
by NimzoRoy 4 years ago
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JVC AV32D305/Y Owner's Manual
Product Reviews
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hi jou - 2008-05-17
Hi, The manual is in perfect condition. Thank you very much.. You are doing an excellent job. Thanks again...
Wojciech Retko - 2008-12-01
Thank you very much. The best party that I met. The excellent quality. I recommend to all. This is my first purchase in this company. For sure not the last! Cheers from Poland!
Radom?r Hason - 2009-01-05
Complete service manual and easy to read,Lots of good info in this one
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Manual received - Many thanks for an excellent service.
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Text excerpt from page 33 (click to view)
Initial Setup
Front Panel Lock
This allows you to lock the keys on the front of the TV, so that a child may not accidentally change your viewing preferences. Press the MENU button Ï� â�
To FRONT PANEL LOCK To turn ON or OFF Press the EXIT button when finished
You can turn off this feature in the following ways: � Unplug the power cord, and plug it back in. Do this if your batteries die, or you lose your remote control. � Use the remote control. � Press the MENU button on the front of the TV for more than 3 seconds. In this case, the OSD for FRONT PANEL LOCK will appear. Note: � To turn ON/OFF the TV, press the power button for more than 3 seconds. This feature will remain ON.
Auto Shut Off
This function automatically shuts off your TV when there is no signal from the channel the TV is on. Press the MENU button Ï� â�
To AUTO SHUT OFF To turn ON or OFF Press the EXIT button when finished
� If the channel that you have on does not receive a signal for more than one minute, the blinking text �NOT RECEIVING A SIGNAL� appears on the screen, and starts the countdown. If no signal is being received within 9 minutes, the TV shuts itself off.
We provide manuals to wide range of devices, including:
Digital Cameras, Gsm, Electronic Organ, Other F-products (mainly Caravan And Pleasure Boat) - Others, Loudspeaker System, Iron, Voice Recorder, Input Card, Floor Polishers (dust, Direct Sales), Refrigeration, Bi,built-in Freezers, Bottom Freezers, Turntable, Cassettendeck, Microwave - 1.2cuft - English/ Spanish, Refrigeration, Bi,built-in Refrigerators > 90 Cm Height, Fm Tuner, Tape Recorders, Freestanding Upright Freezers > 90 Cm Height, Camera Pro, Expansion Kit, System Processor, Av Controller, Cooktop, Interval Analyzer, Cookers - Miscellaneous, Ic Recorder, Cd Drive, Copier Parts Cat., Dvd/cd Writer, Remote Controller, Digital Trax
In our catalog We have following types of manuals:
Concepts Guide, Anleitung, Room Er Owner's Manual, Power Supply Caution, Voice List, Audio Driver, Wdm Sheet, Update, Twp, Calibration Guide, Pattern Book (image), Mlan Guide Book, Adjustments, Display, Studio Manager V2 Installation Guide, User&programmer Vol2, Leaflet(lx01), Owner's Manual With Circuit Diagrams, Wlan Guide, Am, Plasma Caution Sheet, Td-scdma Hsdpa Guide, Vba Programmer, V1.1 Supplement, Hdmi, Auto Doppler Owner's Manual, Dme-n Network Driver Installation Guide For M7cl, Data Sheet, Brief Book, Service Intruction

subset: commoncrawl


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Belfort Furniture offers White Glove in-home delivery and set-up by professionally trained personnel who will unwrap, place, and assemble your furniture in the appropriate room. Delivery is available Tuesday - Saturday. Once your delivery date is scheduled, you will receive a three hour delivery timeframe the day prior to your delivery.
Furniture may be picked up at our warehouse Monday-Saturday from 10AM to 6PM or Sunday from Noon to 5PM. Furniture that is picked-up may require assembly.
For delivery outside of our local metropolitan area, please contact us with your item(s) of interest for a delivery quote! 703-406-7600 or email@example.com
An homage to the changing furniture styles of late 1800's pioneer homes, this impressive seven-drawer chest blends sturdy utilitarian design with a lighter country cottage look. Built with solid reclaimed and recycled wood hand-finished to bring out the character of the wood, this chest infuses your home with rustic authenticity. The casual Sundried Ash finish and simple lines of this piece create a woodsy, relaxed atmosphere.
The Settler 7-Drawer Solid Pine Chest by Four Hands from Belfort Furniture. We proudly serve the Washington DC, Northern Virginia, Maryland and Fairfax VA area. Product availability may vary. Contact us for the most current availability on this product.
The Settler collection celebrates design styles of late 1800's pioneer homes, signifying the transition from heavy, utilitarian pieces to a lighter cottage look. Each piece is bench-built and hand-finished using solid reclaimed or recycled hardwoods. Clean, simple lines and a muted Sundried Ash finish create relaxed, folksy appeal that allows the unique character of the wood to take center stage. Fill your home with rustic charm with pieces from the Settler collection.
The Settler collection is a great option if you are looking for Cottage furniture in the Washington DC, Northern Virginia, Maryland and Fairfax VA area.
These images are representative of this group as a whole and may differ from this item.
Browse other items in the Settler collection from Belfort Furniture in the Washington DC, Northern Virginia, Maryland and Fairfax VA area.
This item has been tagged as: Chests, Storage Chest, Toy Chest, Chest of Drawers, Hope Chest, Drawer Chest, Dresser, Bureau, Furniture, Bedroom Furniture, Bedroom Accent Furniture, Clothing Storage, Gentlemen's Chest, Cabinet, Commode, Trunk, Small Cabinet, Furniture with Drawers, Storage Furniture
Nothing on this page may be copied or reproduced without explicit permission.
Drag anItem Here
To start a new blank room plan, enter the desired room dimensions below and click Create Room.
To request a new password, enter your username or e-mail address and click submit. A new password will automatically be emailed to you.
If you are still having trouble logging on after receiving your new password, please email firstname.lastname@example.com, firstname.lastname@example.com, firstname.lastname@example.com


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Item #BB125074
Little Mermaid Jumbo Add-An-Age Letter Banner (Each)
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Be the first to write a review!
- Size: Banner - 10 1/2 ft.; Letters - 10
Product Description
Welcome guests under the sea for your child's Little Mermaid themed party with this huge banner. This fun string of pink letters that overlay blue seashells features Ariel in a variety of poses on land or in the sea, as well as Flounder and Sebastian! You can personalize this sign to call out the age your child is turning.
Additional Information
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Dynamic Silver Lake duplex offers sun splashed and open living spaces with palm trees and hill views – all within walking distance to the Silver Lake Reservoir! 2244 Fargo, the rear cottage, boasts enormous flexible living spaces, including a large living room with picture windows which ... Read More »
Daily Archives: July 21, 2012
Eastside Open House: A Weekend Guide to House Hunting
This weekend's selection of Eastside open houses ranges from a nearly 5,000-square-foot Silver Lake home with reservoir views (listed at $1.499 million) to a 2-bedroom hillside home in El Sereno for $209,000. There are also some sleek steel-and-glass Atwater Village townhomes and traditional bungalows in Eagle ... Read More »
News & Notes from Boyle Heights, Elysian Valley, Highland Park & Montecito Heights
Downtown View From Whittier Boulevard, Boyle Heights | Photo by Darrin Browne/Flickr Man killed, woman injured in Boyle Heights shooting. L.A. Now Police warn public about Debs Park arsonist. ASNC Alert Graffiti clean up crew defends response times in Highland Park. Patch The Monitor of Montecito ... Read More »


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Gone are the days of going to the library, referencing the card catalogue and then finally retrieving the materials you came for in the first place. Simple and specific searches have now become mainstream and accessible to everyone. The Internet is probably the quickest way to find a relative and many websites offer services for free to help you get in touch with relative you are seeking. If you do not own computer with Internet access, ask a friend if you can borrow theirs temporarily and search the Internet to find the family relative you are seeking.
Things you will need to locate a family relative online for free
A computer
Internet access
Steps you will need to locate a family relative online for free
Step 1
To locate a family relative collect all the information you can gather on the relative for example pictures, marriage, birth and death certificates. This also involves creating a family tree, which is highly beneficial for locating members.
Step 2
Locating family members can be made easy by consulting with immediate and extended family relatives, especially elders in the family. Grandparents, parents and aunts are usually the best source of gossip and relevant information for locating lost relatives. They can also be a good source of information for mapping a family tree.
Step 3
Some web resources online offer free and relevant services for locating lost relatives. Google is the best starting point for finding out information about people, missing or otherwise. Remember to use quotes ("") for an exact search.
Step 4
New social networking sites such as Myspace, Bebo, Hi5 and Facebook are an excellent source of rejuvenating relationships and locating missing family members. Even though they might not directly link to the missing relative, they can ideally link to their ascendants or descendents, offering vital clues in the missing family tree.
Step 5
Phone books can be obvious and easy means of locating relatives. They are an untapped resource of free and publically listed information. Check for online phone book websites to run your search.
1. Google Search Basics
2. Cyndi's List – Finding People
3. Steps to Finding Your Russian Ancestry


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Women get crushed on court in capital city
By: Jonas Swecker
CHARLESTON, W.Va. — The Marshall women's basketball team scored first on two free throws by freshman forward/guard Veronica Ruiz on Wednesday night in the Capital Classic against the No. 13 Mountaineers. It was the only time the Thundering Her…


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Neighborhood barbershop family owned and operated in Hampton Roads.
Colliseum Central
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Veteran stylist and braiding expert travels to clients' homes to design new looks
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Local businesses like this one promote thriving, distinctive communities by offering a rich array of goods and services to locals like you
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Your local stylist is now traveling to meet your haircare needs.
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Huntington Learning Center
770 Road to Six Flags E Suite 170
Arlington, TX
Posted: January 18 2016
Always be on the alert for potentially fraudulent job postings online - never send money to a potential employer.
DescriptionHere at the Huntington Learning Center, we are a team of skill educators who work together to ensure the quality of our student's academic journey. This internship is located right in Lincoln Square, which is remotely close to UT Arlington. This internship entails, working alongside our Assistant Administrator, who also acts as an active teacher in the center. This internship will give the student training in both the administrative field and also the teaching field. We are offering the internship position as early as February.
Responsibilities- Organize and collect progress reports
- Monitor project calendar, ensuring project is on schedule and in budget
-Performs a variety of Internet research functions and uses word processing, spreadsheet and presentation software. Duties also include fielding telephone calls, filing and data entry. May assist with overflow work from administrative and executive assistants and fill in for the office receptionist as needed.
Requirements-Pursuing a Bachelor's Degree in Education
- Possess skills at assessing issues, defining solutions, and implementing strategy
- Perform well in environment that values leadership, efficiency, dependability, and organization


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Giada's pasta recipes are quick and easyPlay Video - 3:02
Giada's pasta recipes are quick and easyPlay Video - 3:02
More video
TODAY's Craig Melvin crafts his own beer at Samuel Adams brewery
Happy National Nut Day! Watch blindfolded TODAY anchors celebrate with a taste test
The ultimate breakfast sandwich: A fried egg with bacon jam and maple syrup
Perfect football-watching food: Coffee-rubbed brisket and black bean chili
Not sure what to make for dinner? Giada De Laurentiis is here to help. She dished out two great pasta recipes, both packed with the fresh flavors of the season, that the entire family will enjoy.


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Total number of EB Games stores in St.Catharines: 4
The biggest mall in St.Catharines with EB Games store: Pen Centre
EB Games stores in St.Catharines - get information about the brand EB Games and all EB Games locations in St.Catharines - Ontario, Canada.
Select EB Games store in St.Catharines from the list below. All EB Games stores are also displayed on the map.
221 Glendale Ave, The Pen Centre, St Catharines, ON L2T 4C4, Canada
420 Vansickle Road, St.Catharines, Ontario, L2R 6P9, Canada
221 Glendale Ave., St. Catharines , Ontario, ON L2T 2K9, Canada
285 Geneva Street, St. Catharines, Ontario, L2N 2G1, Canada
3401 Dufferin Street, Toronto, Ontario - ON M6A 2T9, Canada185 stores
6801, Transcanada Highway, Pointe-Claire, Quebec221 stores
1355 Kingston Road, Pickering, Ontario, Canada, L1V 1B8223 stores
4500 Kingsway, Burnaby, BC V5H 2B1, Canada207 stores
3255 Highway 7 East, Markham, ON L3R 3P9
3035 Boul. Le Carrefour, Laval, Quebec H7T 1C8186 stores
2271 Harvey Ave #180, Orchard Park, Kelowna, BC V1Y, Canada315 stores
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300 Borough Drive, Scarborough, Ontario, M1P 4P5, Canada181 stores
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First published August 8, 2001; 10.1152/ajprenal.00139.2001.—The mechanisms underlying the prevention of age-related polyuria by chronic food restriction were investigated in female WAG/Rij rats. The decreased osmolality of renal papilla observed in senescent rats was not corrected by food restriction. A reduced urea content in the inner medulla of senescent rats, fed ad libitum or food-restricted, was suggested by the marked decrease in expression of UT-A1 and UT-B1 urea transporters. Aquaporin-2 (AQP2) downregulation in the inner medulla of senescent rats was partially prevented by food restriction. Both AQP2 and the phosphorylated form of AQP2 (p-AQP2), the presence of which was diffuse within the cytoplasm of collecting duct principal cells in normally fed senescent rats, were preferentially targeted at the apical region of the cells in food-restricted senescent animals. Plasma vasopressin (AVP) was similar in 10- and 30-mo-old rats fed ad libitum, but was doubled in food-restricted 30-mo-old rats. This study indicates that 1) kidney aging is associated with a marked decrease in AQP2, UT-A1, and UT-B1 expression in the inner medulla and a reduced papillary osmolality; and 2) the prevention of age-related polyuria by chronic food restriction occurs through an improved recruitment of AQP2 and p-AQP2 to the apical membrane in inner medulla principal cells, permitted by increased plasma AVP concentration.
- kidney aging
- phosphorylation
- urea transporters
in mammalian kidney, most of filtered water is reabsorbed through the water channel aquaporin-1 (AQP1) constitutively present in the apical and basolateral membranes of the proximal tubule and thin descending limb (28,35). Final urine is concentrated along the collecting duct by water subtraction owing to the corticopapillary osmotic gradient and water permeability of principal cells, endowed by AQP2 in the apical membrane and both AQP3 and AQP4 in the basolateral membrane. AQP2 targeting to the apical membrane is tightly regulated by arginine vasopressin (AVP), which induces fusion of subapical vesicles containing AQP2 with plasma membrane (13, 27). This process occurs through AVP binding to its V2 receptor in the basolateral membrane, activation of the cAMP signaling pathway, and phosphorylation of AQP2 at serine-256 by protein kinase A (22).
Polyuria with reduced urine osmolality affects elderly humans and rodents (5, 6, 9, 42). In some strains of rats, this age-related defect has been attributed to a loss of nephrons or an impaired AVP secretion (25, 32, 40, 46, 53, 55). However, it is still observed in senescent female WAG/Rij rats that remain free of glomerulosclerosis and have a normal number of nephrons, renal blood flow, and single renal filtration rates (10-12). Plasma AVP concentration, V2-receptor expression, and intracellular cAMP content in the papilla are also maintained in these old rats (15, 30). By contrast, senescent female WAG/Rij rats exhibited a low papillary osmolality and a weak expression of AQP2 and AQP3, whereas expression of AQP1 and AQP4 was unaltered (30). This suggested a low water permeability of the inner medullary collecting tubule, a hypothesis strengthened by the diffused localization of AQP2 within the cytoplasm of papillary principal cells (30).
A reduced papillary osmolality may result from a lower accumulation of NaCl and/or urea within the medulla. Defective sodium accumulation could not be implicated univocally. As a matter of fact, sodium transport in Henle's loop of the cortical nephron was similar in female WAG/Rij rats of 10 and 30 mo of age (11). On the other hand, a reduced AVP-dependent reabsorption of NaCl was found in the medullary thick ascending limb dissected from aging mice (14). This is consistent with the diminished accumulation of cAMP in the same segment of the nephron after maximal AVP stimulation reported in mice and in female WAG/Rij rats (12,21). Accumulation of urea in the medulla is dependent on urea transporters (UTs) (2, 16, 23, 37). UT-A1 is responsible for the AVP-stimulated urea transport, allowing the diffusion of urea from the lumen of terminal inner medullary collecting duct to papilla interstitium (38, 41). UT-A3 has been recently cloned (18, 39) and located in the apical region of terminal collecting duct (45). Its function is still unknown, but its large abundance suggests a role in renal urea handling. UT-A2 and UT-B1 are located in descending structures, respectively, the thin descending limb (29) and descending vasa recta (54). Both UT-A2 and UT-B1 are involved in the medullary recycling of urea. Therefore, it is expected that any age-related change in UT-A1, UT-A2, and UT-B1 expression would modify the corticopapillary gradient and urine osmolality.
Chronic food restriction, which is known to delay the aging process, prevents polyuria and urinary concentrating defect affecting aging female WAG/Rij rats (43). The mechanisms underlying such prevention were presently investigated by measuring water balance, papillary osmolality, plasma AVP concentration, and both AQP and UT expression in senescent rats chronically food restricted by 30% from 10 to 30 mo compared with adult and senescent animals fed ad libitum. To further investigate the recruitment of AQP2 to the apical membrane of principal cells in the inner medullary collecting duct, and the role of phosphorylation in this trafficking, indirect immunofluorescence and electron microscopy were performed to localize the phosphorylated form of AQP2 (p-AQP2).
Animals and food restriction protocol.
Female WAG/Rij rats were born and raised in the animal care facility of the Commissariat à l'Énergie Atomique, Saclay, Gif-sur-Yvette, France, as previously described (30, 43). The WAG/Rij strain is an inbred Wistar rat strain that has been well characterized by Burek (7). Mean lifespan for females is close to 30 mo when they are fed ad libitum. Their body weight increases until they are 6–10 mo old and reaches a plateau corresponding to 180–200 g. From 10 to 30 mo, this body weight is unchanged or slightly increased by 20–40 g (7, 9,43). This prevention of excess weight with age in WAG/Rij rats is, in part, related to their low spontaneous food intake. Measurement of daily food intake in metabolic cages indicates that for a similar body weight, food consumption of WAG/Rij rats is 40% lower than that of the regular Wistar rats (Iffa Credo) (data not shown). In addition, female WAG/Rij rats have an unchanged blood pressure, are free of glomerulosclerosis, and have a constant number of nephrons with age, at least until 30 mo (9). This makes the WAG/Rij rat a suitable experimental model to study the effect of food restriction on kidney aging per se (4, 43).
The animals were maintained on a 12:12-h light-dark cycle, 50% humidity, and a temperature of 21°C. From weaning to 3 mo, the animals were fed a commercial growth diet (DO3, UAR, Villemoisson, France) composed of 9% fish protein, 16% vegetable protein, and a total of 3,500 kcal/kg. At the age of 3 mo, they were switched to a diet containing 2% fish and 15% vegetable protein, 0.71% phosphate, 0.78% calcium, 0.62% potassium, 0.27% sodium, 0.22% magnesium, and a total of 2,900 kcal/kg (DO4, UAR). The ad libitum food intake of animals was close to 10 g/day, regardless of age (43). Chronic food restriction consisted of offering 7 g/day of the same diet to rats, a 30% restriction, from 10 to 30 mo of age, at which time they were killed. During this period of time, the survival curves were similar for food-restricted and ad libitum fed rats; i.e., 36% of the animals had died at 30 mo in both groups (43). A short-term 30% food restriction was performed for 1 wk in 10- and 30-mo-old rats for comparison with the long-term protocol.
Metabolic cage data.
Daily water and food intake, as well as urinary volume, were measured for a 24-h period after 2 days of acclimatization to metabolic cages. Urine was collected under oil to avoid evaporation, and osmolality was determined with a Roebling Automatik osmometer.
Measurement of plasma AVP, plasma osmolality, and papillary osmolality.
Blood and kidneys were collected immediately after decapitation of rats. Plasma AVP concentration was measured by radioimmunoassay with antiserum K9-IV (a gift of Dr. L. C. Keil, National Aeronautics and Space Administration Ames Research Center; Ref. 19) and 125I-labeled iodo-AVP (15, 19). The minimum sensitivity of the assay was 0.25 pg/assay. For each animal, determination of plasma AVP concentration was performed in duplicate on three occasions (with independent standard curves). Plasma osmolality was measured on the same samples with a Fiske one-ten osmometer. The whole white papilla was excised from each kidney, weighed, and homogenized manually with a glass potter after addition of 200 μl of distilled water. The osmolality of the homogenates was measured with a Roebling Automatik osmometer as previously described (30). Papilla osmolality was calculated on the assumption that 80% of the wet weight is water (1).
UT-A1 and UT-A2 were revealed with an affinity-purified rabbit polyclonal antibody directed against the COOH-terminal 19-amino acid sequence common to rat UT-A1 and UT-A2 (Alpha Diagnostic International, San Antonio, TX). UT-B1 was detected with a rabbit polyclonal antibody raised against a synthetic peptide from the NH2-terminal sequence of rat UT-B1 (Neosystem, Strasbourg, France) (17). AQP2 and AQP3 were detected with polyclonal antibodies raised against the COOH-terminal peptide sequence of rat AQP2 and rat AQP3 (3, 33). An affinity-purified antibody against p-AQP2, AN244-pp-AP, was prepared using a peptide corresponding to amino acids 253–262 of rat AQP2 phosphorylated at serine-256, as previously described (8).
Western blot analysis.
Fragments of the inner stripe of the outer medulla and of the inner medulla were dissected on ice from each kidney and were homogenized manually with a glass potter in 500 μl of PBS, pH 7.4, containing 10−3 mol/l phenylmethylsulfonyl fluoride. Protein concentration of the crude homogenates was determined by the method of Bradford using a protein assay reagent kit (Bio-Rad, Hercules, CA). Samples were then diluted to a final concentration of 0.5 mg/ml, solubilized in Laemmli buffer, and heated at 65°C for 10 min. Proteins were separated by SDS-PAGE (12% for AQPs and 10% for UTs), and immunoblotting was performed as previously described (30). The membranes were blocked for 30 min at room temperature with PBS supplemented with 5% nonfat dry milk, followed by incubation with the primary polyclonal antibody for 1 h at room temperature. The membranes were then washed and incubated with a peroxidase-conjugated anti-rabbit IgG polyclonal antibody (0.2 μg/ml, Promega, Madison, WI) before visualization on Hyperfilm-ECL (Amersham) by enhanced chemiluminescence (ECL, NEN, Boston, MA). After scanning, semiquantitative densitometry was performed using Molecular Analyst software (Bio-Rad). Mean density for senescent rats, fed normally or food restricted, was expressed as a percentage of that obtained from adult rats. Equal protein loading was verified by Coomassie blue staining of the membranes at the end of the experiment.
For indirect immunofluorescence, the kidneys were fixed by perfusion at a pressure of 120 mmHg through the abdominal aorta with a PBS solution containing 4% paraformaldehyde warmed to 37°C. The kidneys were sliced, postfixed overnight in PBS-4% paraformaldehyde, and washed extensively with PBS. Fixed kidney slices were infiltrated overnight in 30% sucrose and frozen in liquid nitrogen; then, 4-μm sections were collected on SuperFrost+ glass slides. Sections were preincubated in PBS containing 1% BSA (PBS/BSA) for 5 min, then incubated for 1 h at room temperature in PBS/BSA containing antibodies against AQP2 (1:300), p-AQP2 (1:50), or AQP3 (1:200), and washed for 3 × 10 min in PBS. Sections were then incubated for 45 min at room temperature in FITC-conjugated goat anti-rabbit antibodies (10 μg/ml, Promega), washed for 3 × 10 min, and mounted in 50% glycerol solution containing 2% N-propyl-gallate before observation under a fluorescence microscope (Olympus Van OX).
Electron microscopy.
Kidneys were fixed by perfusion of PBS containing 4% paraformaldehyde and 0.1% glutaraldehyde, sliced, postfixed overnight in the same solution, and washed several times with PBS. Tissue pieces from the inner medulla were dehydrated in ethanol and embedded in unicryl, and 90-nm sections were then cut with an ultramicrotome and collected on electron microscopy grids. Sections were preincubated for 30 min at room temperature in 20 mM Tris buffer (pH 7.5) containing 0.1% BSA, 0.1% fish gelatin, 0.05% Tween 20, incubated in Tris buffer for 2 h with the anti-p-AQP2 antibody (1:100), and washed for 6 × 5 min with Tris buffer. Grids were then incubated in 10-nm colloidal gold-conjugated goat anti-rabbit antibodies in Tris buffer for 1 h and washed 6 × 5 min with Tris buffer. Sections were stained and observed under an electron microscope (Philips EM 400).
Results were expressed as means ± SE, and differences were analyzed by ANOVA or Student's t-test. Statistical significance was considered at P < 0.05.
Effect of chronic food restriction on water balance and urine osmolality.
From 10 to 30 mo, body weight significantly increased from 205 ± 5 to 245 ± 10 g in animals fed ad libitum (n= 8 in each group). In food-restricted rats, body weight progressively decreased from 10 to 30 mo, to reach a final value of 152 ± 4 g (n = 8), that is a 50-g loss. These data are consistent with those obtained on a larger cohort of animals, as recently published (43). Data from metabolic cage experiments are summarized in Table 1. Food intake was comparable in 10- and 30-mo-old rats fed ad libitum, as was total solute excretion. In control animals, urine flow rate increased more than twofold from 10 to 30 mo, and urine osmolality was reduced in proportion. The age-related loss of water was compensated for by a larger water intake in senescent rats. Chronic food restriction prevented the age-related changes in water balance. Urinary volume and osmolality were similar in food-restricted 30-mo-old rats and 10-mo-old normal animals. Food-restricted senescent rats had a lower water intake than unrestricted senescent rats and even lower than unrestricted adult rats. Total solute excretion was diminished by 30%, the same proportion as food intake.
Short-term food restriction, performed for 1 wk in 10-mo-old animals, significantly reduced water intake and urine osmolality, whereas urine flow rate was unchanged (Table 1). Short-term food restriction for 1 wk in 30-mo-old rats significantly reduced water intake and urine flow rate but did not prevent the age-related kidney concentrating defect (Table 1).
Plasma AVP, plasma osmolality, and papillary osmolality.
Plasma osmolality and AVP concentration were unchanged from 10 to 30 mo in normally fed rats (Table 2). Long-term food restriction doubled plasma AVP concentration and increased plasma osmolality in senescent animals (Table 2). In animals fed ad libitum, osmolality of whole papilla was significantly lower in 30-mo-old compared with 10-mo-old rats (645 ± 48 and 1,084 ± 103 mosmol/kg H2O, respectively, n = 14/group). Food restriction did not prevent this age-related decrease in papillary osmolality (678 ± 35 mosmol/kg H2O, n= 17).
UT-A1, UT-A2, and UT-B1 expression.
In renal inner medulla, the 97-kDa form of UT-A1 and UT-B1 expression was massively reduced (by 90%) in senescent rats fed ad libitum, and this effect was maintained in food-restricted animals (Fig.1). The 117-kDa isoform of UT-A1 was also underexpressed but less intensively than the 97-kDa form.
Under normal feeding conditions, UT-A2 expression in renal outer medulla was significantly higher in old than in adult rats, whereas UT-B1 expression was similar at the two ages (Fig.2). Food restriction tended to lower UT-A2 abundance toward that found in adult rats and induced a slight increase in UT-B1 expression (Fig. 2).
AQP1, AQP2, AQP3, AQP4, and p-AQP2 expression.
AQP2 expression was reduced with age by 90% in rats fed ad libitum and by 75% in chronically food-restricted animals (Figs.3 A and 6). Although AQP2 expression was twofold higher in food-restricted than in unrestricted 30-mo-old rats, the difference was not statistically significant (Figs.3 B and 6). AQP3 expression was decreased by 25% in senescent animals. This change was prevented by chronic food restriction (Figs. 4 and 6). However, the difference between control and restricted 30-mo-olds rats was not statistically significant. Expression of p-AQP2 was decreased by 90% in 30-mo-old rats fed ad libitum compared with 10-mo-old rats (Figs.5 and 6), and by 85% in food-restricted senescent animals. The difference in p-AQP2 expression was not significant between food-restricted and rats fed ad libitum (Figs. 5and 6). AQP1 and AQP4 expression, unaltered from 10 to 30 mo in normally fed rats, was not significantly modified by food restriction (Fig. 6).
AQP2, AQP3, and p-AQP2 cellular localization.
In adult rats, AQP2 was mostly localized in the apical region of inner medullary collecting duct (Fig.7 A, a). In senescent rats fed ad libitum, AQP2 distribution was faint and diffuse within the cytoplasm of principal cells (Fig. 7 A,b). In food-restricted senescent rats, the staining was still weak, as expected from immunoblotting data, but AQP2 was totally addressed to the apical membrane region of principal cells (Fig.7 A, c). A staining similar to that of AQP2 was found by indirect immunofluorescence for p-AQP2 in the three groups of rats (Fig. 7 B). Electron microscopy in adult rats showed that p-AQP2 was present mostly in the apical membrane of principal cells, but some labeling was also observed in subapical vesicles (Fig.8 a). Localization of p-AQP2 in the apical membrane was reduced in senescent rats fed normally (Fig.8 b), in contrast to food-restricted senescent rats, in which it was even intensified compared with adult rats (Fig. 8 c).
Regarding AQP3, no significant difference in the intensity of the staining or in the cellular distribution of AQP3 was observed with age in rats fed normally, despite decreased expression observed by immunoblotting (data not shown). Indirect immunofluorescence staining of AQP3 was similar in food-restricted senescent animals compared with the other two groups (data not shown).
The present study shows that chronic food restriction prevents polyuria and urinary concentrating defect in aging rats. In contrast, acute food restriction, which reduces urinary volume in adult and senescent rats, does not significantly affect the age-related decrease in urine osmolality. The corresponding molecular mechanisms involve a preferential targeting of AQP2 and p-AQP2 to the apical membrane of principal cells in the inner medullary collecting duct, in relation to a doubling of plasma AVP. In addition, AQP3 expression is increased in food-restricted rats, but AQP2 and p-AQP2 expression levels remain low. The low papillary osmolality in senescent rats is maintained during food restriction, consistent with low expression of UT-A1 and UT-B1.
Multiple factors could be involved in defective urine concentrating ability with age. They include dilution capacity of the thick ascending limb, AVP secretion, V2 receptor signaling, and corticopapillary gradient, as well as AQP and UT expression. In the present experimental model, which remains free of glomerulosclerosis and has a constant number of nephrons, impaired plasma AVP secretion and signaling are not the primary cause of defective urinary concentration (15). An abnormal number of V2receptors or cAMP content in inner medulla can be also excluded (21, 30). In contrast, papillary osmolality is significantly reduced with age. In rodents and mammals, NaCl and urea contribute to the papillary hypertonicity required for water conservation. The expression of Na-K-2Cl transporter in senescent rat kidney is not documented, although previous studies have already suggested a reduced AVP-dependent NaCl reabsorption with age in rodents (14, 21). Urea accumulation in the renal medulla is permitted by urea transporters (36, 47, 50). It is initiated by the diffusion of urea from the terminal collecting duct lumen to the papillary interstitium, owing to the presence of UT-A1. UT-A2 and UT-B1, present in the thin descending limbs and the descending vasa recta, respectively, trap part of the urea escaping from the papilla, by countercurrent exchange with ascending vasa recta, and return it to the inner medulla. The present study shows that defective expression of UT-A1 and UT-B1 likely contributes to the observed low corticopapillary osmotic gradient of senescent rats. In addition, it reveals that the features of changes in senescence-induced polyuria are totally different from other polyuric situations.
In old rats, both 97- and 117-kDa glycosylated isoforms of UT-A1 (6a) are underexpressed. This pattern of changes differs from water loading, furosemide treatment, or low-protein diet, where 117-kDa is increased and the 97-kDa isoform is unchanged, especially in the tip of the inner medulla (44, 48, 49). The mechanisms underlying UT-A1 underexpression with age are still unknown. However, it can be speculated that high glucocorticoid levels reported in senescent rats would contribute to this downregulation, inasmuch as providing glucocorticoids to adrenalectomized rats results in a downregulation of terminal collecting duct urea permeability and UT-A1 protein (20, 26). The restoration of urine concentration by food restriction is not associated with normalization of UT-A1 expression.
Regulation of UT-B1 by urine concentrating activity is poorly documented. UT-B1 is also strongly downregulated in the inner medulla of senescent rats but not in the outer medulla. This change is opposite to that found in AVP-lacking Brattleboro rats that exhibited an UT-B1 mRNA abundance higher in inner medulla but lower in outer medulla than in AVP-infused Brattleboro rats (31).
UT-A2 expression in the thin descending limbs was upregulated in rats fed ad libitum but not in food-restricted senescent rats. An upregulation of UT-A2 expression was previously reported in Brattleboro rats treated with AVP (31, 52). This was mediated by an increase in medullary osmolality (24, 48) due to AVP. Unexpectely, in the present rat model, neither AVP nor medullary osmolality regulated UT-A2 abundance. In normally fed senescent rats, UT-A2 was upregulated without elevation of AVP plasma levels and with decreased inner medullary osmolality.
In food-restricted senescent rats, UT-A2 expression remained constant although both AVP levels and urine osmolality were increased. In senescent rats, urea reabsorption in inner medullary collecting ducts by UT-A1 is likely low, and medullary urea recycling cannot occur efficiently. In 30-mo-old rats, the increased UT-A2 expression may contribute to medullary urea dissipation instead of accumulation by bringing to medullary collecting ducts an amount of urea that cannot be captured by UT-A1 and that will be lost in urine. In food-restricted 30-mo-old rats, the absence of UT-A2 upregulation could minimize this adverse effect and hence contribute to the prevention of urinary concentrating defect.
In the aging kidney, in addition to a reduced papillary osmolality, a decrease in transepithelial water permeability of papillary collecting duct is suggested by AQP2 and AQP3 downregulation and AQP2 diffused localization in the cytoplasm of principal cells (30). Chronic food restriction prevents the decreased expression of AQP3 with age but not that of AQP2. However, chronic food restriction induces in senescent rats a recruitment of AQP2 to the apical membrane of principal cells. This membrane recruitment may restore papillary collecting duct water permeability, which would be lost with age. This greater transepithelial permeability is consistent with the enhanced AQP3 expression which, with AQP4, contributes to the water permeability of the basolateral membrane of principal cells.
AVP-dependent insertion of AQP2 in the luminal membrane is the main mechanism for control of water permeability in collecting duct principal cells. AVP binding to basolateral V2 receptor stimulates cAMP generation from adenylyl cyclase, which is responsible for the fusion of subapical vesicles containing AQP2 to the apical membrane of the cells. Plasma osmolality and plasma AVP are unchanged from 10 to 30 mo (15, 30), but they are significantly increased in long-term food-restricted senescent rats. This chronic higher plasma AVP level may be responsible for the marked recruitment of AQP2 to the apical membrane. Besides, without being fully restored, the expression of AQP2 is weakly increased, maybe via cAMP-dependent transcriptional regulation (51).
Long-term food restriction is associated with a 30% decreased water intake in 30-mo-old rats. This mild chronic dehydration may be responsible for the higher plasma osmolality and AVP level observed in these rats. Food restriction in senescent rats induces a decreased water consumption both in long-term and short-term protocols. Short-term reduction of water intake does not improve the age-related kidney concentrating defect. Therefore, chronic food restriction may be an adaptive process, involving a mild water restriction and an increased plasma AVP release. However, the present study cannot rule out that the improvement of urinary concentrating ability in the aging kidney by long-term food restriction may be due directly to the effect of chronic reduction on water intake.
AVP-induced phosphorylation of AQP2 at serine-256 by protein kinase A is critically involved in its localization in the apical membrane. As described before in normal rat kidney (8), p-AQP2 has been detected, in the present study, both in apical plasma membrane and in intracellular vesicles in noninduced principal cells of adult and senescent rats fed ad libitum. In chronic food restriction, p-AQP2 is also localized in subapical vesicles of collecting duct principal cells but is strongly recruited in apical membrane. The expression of p-AQP2 is not significantly modified despite the AVP increase in chronic food restriction, consistent with the unchanged p-AQP2 level in normal rats treated with [desamino-Cys1,d-Arg8]AVP (8).
In summary, chronic food restriction prevents kidney concentrating defect in female WAG/Rij rats. However, it cannot be excluded that this effect may be secondary to the long duration of the mild water intake depletion associated with food restriction. The molecular mechanisms of this urinary concentrating ability rescue in aging kidney involve an AVP-dependent recruitment of AQP2 and p-AQP2 to the plasma membrane of principal cells, independently of the expression of AQP2 and UTs or changes in the corticopapillary osmotic gradient. This favors a dual mechanism for the control of water homeostasis in aging rats: one depending on plasma AVP level and related to membrane targeting of AQP2 and p-AQP2 and another associated with changes in AQP2 and UT expression and with a low corticopapillary gradient, independent of AVP level.
We thank Drs. S. Le Maout and F. Tacnet (CEA, Gif-sur-Yvette, France) for providing us with AQP2 and AQP3 antibodies and Ferring Pharmaceuticals (Malmö, Sweden) for the gift of arginine-AVP. We are grateful to J.-C. Robillard and P. Héry for outstanding animal care and to G. Augoyard for skills in performing AVP assays.
This work was funded by a Training and Mobility of Research ERBFMRXCT970128 from the European Union. L. Teillet was supported by a grant from the Assistance Publique-Hôpitaux de Paris, Direction de la Recherche Clinique, Paris, France.
Address for reprint requests and other correspondence: J.-M. Verbavatz, Service de Biologie Cellulaire Bat. 532, CEA/Saclay, F-91191 Gif-sur-Yvette cedex, France (E-mail: jmverbavatz{at}cea.fr).
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
First published August 8, 2001;10.1152/ajprenal.00139.2001
- Copyright © 2001 the American Physiological Society


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On my bowed skull plants her black flag.
Monopoly bro, shits a classic.
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Electroneurogram (ENG) recording techniques benefit from the use of tripolar cuffs because they assist in reducing interference from sources outside the cuff. However, in practice the performance of ENG amplifier configurations, such as the quasi-tripole and the true-tripole, has been widely reported to be degraded due to the departure of the tripolar cuff… (More)
—This paper describes the design of an adaptive control system for recording neural signals from tripolar cuff electrodes. The control system is based on an adaptive version of the true-tripole amplifier configuration and was developed to compensate for possible errors in the cuff electrode balance by continuously adjusting the gains of the two differential… (More)
— This work presents an eight channel neural stimulator interface based on an address event communication protocol. The system developed decodes, integrates and interleaves the spike patterns using a continuous interleave sampling (CIS) strategy, which drives a complex biphasic current waveform to a bipolar electrode configuration. This paper describes the… (More)
The presence of cuff imbalance degrades the signal-to-interference (ENG/EMG) ratio in tripolar nerve cuff electrode recordings. Known causes of cuff imbalance include inhomogeneous tissue growth after cuff implantation and cuff manufacturing tolerances. In this paper, we report on an additional contribution to cuff imbalance that stems from variations in… (More)
Current drivers are fundamental circuits in bioimpedance measurements including electrical impedance tomography (EIT). In the case of EIT, the current driver is required to have a large output impedance to guarantee high current accuracy over a wide range of load impedance values. This paper presents an integrated current driver which meets these… (More)


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Lesbian Teacher Sex: Seduced In The Shower
When her games teacher offers her extra help with her volleyball training, the lesbian woman doesn't suspect that the she has more in mind than just practice. Her suspicions are aroused at the close attention and touches her teacher uses during training and her suspicions are confirmed when the teacher joins her in the shower afterwards. Excited at the prospect of making love with a mature woman, the girl lets the teacher seduce her for some wet lesbian shower sex.
Read alsoLesbian Lactation Sex: Milked By The Boss
A young woman doesn't expect to get an easy time from her demanding lesbian boss when she returns to work soon after giving birth. She finds that the woman shows an interest in her fuller breasts though and decides to see if she can use them to her advantage. Letting her boss catch her pumping milk in the bathroom, she realizes she is right. She…


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Rooms, services Spend some remarkable days in Dublin and enjoy the hospitality of Belvedere Hotel Dublin! The hotel is offering 92 comfortable rooms to guests looking for accommodation in Dublin. If you feel like having a meal not included in your chosen type of catering, you can always taste local dishes in the restaurant of the hotel. Car rental service and organized city tours are also available to discover all must-sees and highlights of the area. If you wish to spend your leisure time actively, you may also take the nearby opportunities to go sailing and rent a boat. The café or the bar can be an ideal place for a meeting or a pleasant evening talk. Your valuables can be the safely secured in the safe. Wireless Internet connection (WiFi) is available at the hotel, ensuring that you can stay up-to-date during your holiday. The exact equipment and the exact list of available services of Belvedere Hotel Dublin can be read on the information sheet below.
Payment Advance payment by credit card or providing credit card guarantee may be necessary for finalizing the reservation, depending on the chosen date, room and price.
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Explore these ideas and much more!
به رمزی گفتم این معنی به مفهوم شود آن را که باشد عقل معلوم
~dare to be different. it's so much more fun!!* This was me Kinderguarden Chrostmas concerto :D
Top 30 Best Inspirational Quotes.... I will stop saying "go with the flow" dead fish make me sad.
I get you, Carl Jung. It is so much easier to blindly judge rather than look at situations from different perspectives.
Each phase of my life brings a more understanding of what I want my life story to be , how I'm remembered and how I treat those around me . My choices in life weren't always best for those I love so with each day I need/ am more conscience of my actions because those actions just doesn't effect me but others that I'm suppose to protect !


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Near-miss that could have saved the Titanic: Liner nearly crashed leaving docks, which would have prevented doomed voyage
It was the worst of omens for the gleaming new ship embarking on its maiden voyage.
As the Titanic left the Southampton docks, it came within two feet of crashing into another liner.
Had the two actually collided, it would have cut short the Titanic's maiden voyage and averted the catastrophe that was to claim 1,517 lives.
Close call: The Titanic (large liner, right) narrowly avoids a collision with the New York (large liner, left) at Southampton docks
The near-miss was down to an accident-prone captain and the narrow channel being cluttered with ships moored due to a coal strike, a new book reveals.
In 101 Things You Thought You Knew About The Titanic ... But Didn't, author Tim Maltin claims Captain Edward Smith, despite being an experienced skipper, was only used to boats half the size of the 50,000-ton Titanic when he brought it perilously close to the SS City of New York passenger liner berthed in Southampton.
Captain Smith had previously crashed its sister ship, the Olympic, into the dock in New York.
The author added: 'The collision was avoided by about two feet, something which obviously is an indicator of the disaster to come. He was the most experienced captain in the north Atlantic by a long way. Anyone with a ship that size would be accident-prone - the world had never seen a ship that big before.'
The liner sank in the Atlantic on April 15, 1912, after hitting an iceberg.
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The Great Sinner (Full Screen)
Gregory Peck,
Ava Gardner,
Walter Huston,
Melvyn Douglas &
Ethel Barrymore
Robert Siodmak
Warner Archive Collection (series)
Starring: Gregory Peck, Ava Gardner, Walter Huston, Melvyn Douglas & Ethel Barrymore Director: Robert Siodmak
Drawn to an alluring beauty he meets aboard a train, a Russian writer follows her into an elegant casino. There he is intrigued by the obsessive life she and her father share, risking their fortunes and their futures on the spin of a roulette wheel.
|List Price:
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|$6.86 (38% Off)
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Format: DVD-R
sku: WAC 628845D
Related products:
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DVD Features:
- Rated: Not Rated
- Run Time: 1 hours, 50 minutes
- Video: Black & White
- Released: December 13, 2011
- Originally Released: 1949
- Label: Warner Archive Collection (MOD)
- Encoding: Region 0 (Worldwide)
- Aspect Ratio: Full Frame
- Audio:
- PCM Mono - English
Performers, Cast and Crew:
|Gregory Peck, Ava Gardner, Walter Huston, Melvyn Douglas & Ethel Barrymore
|Melvyn Douglas, Walter Huston, Agnes Moorehead, Frank Morgan & Ethel Barrymore
|Directed by
|Robert Siodmak
|Edited by
|Harold F. Kress
|Screenwriting by
|Christopher Isherwood
|Screenplay by
|Ladislas Fodor & Christopher Isherwood
|Composition by
|Bronislau Kaper
|Art Direction by
|Cedric Gibbons & Hans Peters
|Produced by
|Gottfried Reinhardt
|Director of Photography:
|George J. Folsey
|Dick Simmons
Description by OLDIES.com:
Drawn to an alluring beauty he meets aboard a train, a Russian writer follows her into an elegant casino. There he is intrigued by the obsessive life she and her father share, risking their fortunes and their futures on the spin of a roulette wheel. At first the writer is a mere observer. But soon he is drawn inexorably into a madness that threatens to destroy him.
An all-star cast headed by Gregory Peck and Ava Gardner headlines this lush period drama inspired by Fyodor Dostoyevsky's The Gambler, etching portraits of sinners great and small caught in a vortex of self-destruction.
Product Description:
Based loosely on the Dostoyevsky novel, THE GAMBLER stars Gregory Peck as a sensitive 19th-century Russian author. His "great sin" is gambling, which starts when he attempts to rescue aristocratic Ava Gardner from the gaming tables. He succeeds, only to lose himself to gambling fever, which costs him his friends, his reputation and his talent. Director Robert Siodmak was never happy with the screenplay for THE GREAT SINNER, constant revisions bloated the film's rough-cut running time to nearly six hours! After Siodmak pared the film down, MGM insisted that the director reshoot the love scenes. Siodmak refused, thus the new sequences were filmed sans screen credit by Mervin LeRoy.
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The Baltimore Ravens' stocky nose guard represents the definition of a blue-collar football player. Gregg wrangles with massive offensive lineman at the line of scrimmage, using the techniques of leverage taught to him as a national champion junior heavyweight wrestler.
Gregg collapses the pocket with a determined charge. He racks up tackles. He bleeds, and draws blood. And the former University of Oklahoma standout is often underestimated because of his stout build at 6-foot and 310 pounds.
He appears even shorter because he's so compact, built somewhat like a fire hydrant. "He is just the unsung man that everybody figures, 'How can this guy keep playing pro football,'" Ravens coach Brian Billick said. "He's too short. He's too this, he's too that and all he does is make plays and work his butt off for us. He's a big part of what we're doing."
In the violent scrum in the interior of the line, Gregg thrives primarily because of his brute strength and uncommon quickness. He bench presses 550 pounds, qualifying him one of the strongest Ravens. At the snap of the football, Gregg immediately jams his palms into the center's chest and begins pushing forward, disengaging from the confrontation to pursue the ball.
In his first season as a starter last year, Gregg led the defensive line with 84 tackles to go along with two sacks. "I think Kelly has proved he can play on this level or any other," said Ravens defensive line coach Rex Ryan, who coached Gregg in college. "He's short, that's all. He's not small. I know he doesn't look the part, but he's got all the intangibles that you look for. He never has a poor game."
Against the New Orleans Saints last season, Gregg had 10 tackles with a sack. Mostly, Gregg gets by on hustle and a relentless attitude. The questions about his size don't faze him. "I love that guys underestimate me because of my height and that works to my advantage," Gregg said. "People are always trying to bring you down, but if you do what you are supposed to do who cares about your size? "I don't have a chip on my shoulder about it because the people whose opinion I care about are teammates and family, not critics."
At Edmond High School (Okla.), Gregg won three state wrestling championships and he routinely whipped the Sooners' heavyweights in college although he never joined the team. His wrestling background continues to come in handy. "Playing nose guard is very similar to what I did in wrestling," Gregg said. "It's all about leverage and balance. The lowest man wins. You get under people's pads and you never underestimate anybody. Being a wrestler is just like being in the trenches." Added Ryan: "When you go back in the history of the game, most of your great nose tackles were great wrestlers."
Although Gregg is one of the most approachable Ravens off the field, he possesses a mean streak on it as offensive guard Mike Collins learned last summer. Gregg responded to what he considered a cheap shot by Collins by bulldogging him to the ground and bouncing the back of his head off the ground several times. It took several players to pull Gregg off of Collins. "Kelly's a great guy, but everybody's got that wick and when it burns out Kelly is no one you want to mess with," said Ryan, who recommended signing Gregg the day after the Philadelphia Eagles cut him in 2000 when he didn't want to accept a demotion to the practice squad.
Retired Baltimore defensive tackle Tony Siragusa coined the nickname, 'Buddy Lee' for Gregg after the figurine in a national jeans commercial. The moniker has stuck. Now, that Siragusa has retired and Sam Adams and Lional Dalton have long since departed, Gregg is the Ravens' main man in the middle of the line with depth provided by Maake Kemoeatu and rookie Aubrayo Franklin. "I just keep working hard," Gregg said. "If I wasn't playing in the NFL, I'd be trying to get in a game back home on the sidewalk."
Aaron Wilson writes for the Carroll County Times.


text:
I waited to broach this subject until the MSM driven hysteria calmed down so a reasonable approach could be talked about. The MSM used their leftist agenda of "Rich white men are evil" to taint their telling of the story! Unfortunately for Donald Sterling and those who wish for racial harmony, the MSM got the entire story wrong, this wasn't about racism, it is about the tragedy of Geriatric Dementia and the problems that follow it.
Donald Sterling is the 84 year old billionaire owner of the Los Angeles Clippers basketball team. Obviously in his life he attained a huge amount of success, at least at the business of increasing his wealth. Never during this time has there been any accusations of malfeasance. He had been recognized a number of times for his charitable giving, even the NAACP had awarded him 2 honors and had him up for a third when the scandals broke.
Then his journey into the darkness of Geriatric Dementia began. The first signs are he hired an assistant 50 years his junior with no experience in the field. This young lady known as "V" was a definite trollop and gold digger looking to increase her bottom line. Donald in his deteriorating mental state fell for her. He not only paid her well, he also bought her jewelry and paid for her living expenses. The total incentive package was estimated at over $250,000 in a short time period.
It sure does appear that V was clearly baiting Donald with her antics bringing other men to Clippers games and using Donald's courtside seats. Yes he objected, he viewed V as his concubine since he was paying for her. His wasn't a racial slur it was a juvenile rage of jealousy. Note he didn't complain about her bringing women of various ethnic backgrounds only about the men.
Which brings us to the actual taping. Why was V taping the conversations if not to use for extortion purposes? But Barbara Walters didn't think to ask about that, she was too caught up in proving racism than in getting the actual story.
All the reactions from the Media Frenzy to the NBA Commissioner were overboard and misdirected. MSM should be fined by the FCC for creating mass hysteria with their over hyped message of racism!
V should be jailed for elder abuse, grand theft larceny, extortion, and prostitution. The MSM and all its talking heads should also be charged as accessories to extortion, and elder abuse. The NBA Commissioner should be charged with elder abuse. The other NBA owners should be very cautious as to how they proceed as they could be the next one vilified. Fire the commissioner now before he makes one of you owners his next victim.
Work Hard Have Fun!
Bob DeMaura


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Wellington - Troubled New Zealand cricketer Jesse Ryder was handed a six month ban on Tuesday after testing positive for outlawed substances contained in a weight-loss supplement, the New Zealand Sports Tribunal said.
The tribunal said Ryder, who has been in self-imposed exile from international cricket since February 2012, tested positive after a domestic match in March this year.
It said Ryder's sample showed traces of the stimulants PBA and DEBEA and the batsman admitted he took two supplement tablets in the days before the Test.
The disciplinary body said it accepted Ryder's argument that he was not aware the over-the-counter product contained banned substances and he took it because he was struggling with his weight, not to enhance his on-field performance.
However, it said he did not check with authorities whether the supplement was legal, even though the label warned it might contain substances outlawed by some organisations.
Instead, Ryder and his trainer did their own research on the Internet and concluded it was safe.
The tribunal said that, as a professional athlete, Ryder was obliged to be more cautious before taking a potentially illegal supplement.
"The tribunal thought a penalty of six months suspension was also appropriate," it said, pointing out the maximum punishment available was two years.
The ban was backdated to April, meaning Ryder will be available to play again in October.
The 29-year-old is one of New Zealand's best batting talents, averaging 40.93 in 18 Tests, but his career has been marred by disciplinary lapses and off-field problems.
He is still recovering from an assault outside a bar in the South Island city of Christchurch in March, which derailed his plans to return to the Indian Premier League.
Ryder walked away from international cricket last year, saying he needed to sort out "personal issues" and has so far resisted calls from pundits and fans for his return.
New Zealand Cricket declined to comment.
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