text:
|By PR Newswire
Article Rating:
|September 4, 2014 01:47 PM EDT
WASHINGTON, Sept. 4, 2014 /PRNewswire-USNewswire/ -- On Tuesday, Sept. 9, Atlas Network will host a special event in San Francisco to highlight the spirit that took down the Berlin Wall, and how that spirit can be reignited across the globe to tear down today's Berlin Walls.
Atlas Network CEO Brad Lips said, "Today it is hard to remember how the Berlin Wall – that barrier to freedom that held half a continent captive – seemed absolutely permanent… until it wasn't. Barriers to freedom still exist all over the world. Many seem permanent. But Atlas Network partners are working to tear down – one by one – today's Berlin Walls."
The event will feature Garry Kasparov – legendary chess champion, Russian political activist, Putin foe, and the 2014 Atlas Templeton Leadership Fellow.
Other speakers include John Fund, National Review columnist; Deroy Murdock, Fox News contributor; Naomi Brockwell, creator of BitcoinGirl.org; Dr. Tom G. Palmer, Atlas Network's executive vice president and editor of the new book Peace, Love, & Liberty; Sally Pipes, Pacific Research Institute's president and CEO; Marek Tatała, economist at FOR (Civil Development Forum) in Warsaw, Poland; and Robin Sitoula, founder and executive director of Samriddhi, The Prosperity Foundation in Nepal.
Who: Garry Kasparov, John Fund, Tom Palmer, Naomi Brockwell, and more
What: Tearing down today's Berlin Walls Event sponsored by Atlas Network, Pacific Research Institute and The Independent Institute
When: Tuesday, Sept. 9, noon to 4:30 p.m.
Where: Marines' Memorial Club and Hotel, 609 Sutter Street, San Francisco, CA 94102
This event is open to media and will have various expert availability throughout the event.
Media must RSVP to Daniel Anthony
Public may register for the event at AtlasNetwork.org
About the event sponsors:
Atlas Network is based in Washington. It is a nonprofit organization connecting a global network of more than 400 free-market organizations in over 80 countries to the ideas and resources needed to advance the cause of liberty.
The Pacific Research Institute is based in San Francisco. It champions freedom, opportunity, and personal responsibility for all individuals by advancing free-market policy solutions.
The Independent Institute is based in Oakland. It boldly advances peaceful, prosperous, and free societies grounded in a commitment to human worth and dignity.
Photo - http://photos.prnewswire.com/prnh/20140904/143131
SOURCE Atlas Network
In his general session at 18th Cloud Expo, Lee Atchison, Principal Cloud Architect and Advocate at New Relic, discussed cloud as a 'better data center' and how it adds new capacity (faster) and improves application availability (redundancy). The cloud is a 'Dynamic Tool for Dynamic Apps' and resource allocation is an integral part of your application architecture, so use only the resources you need and allocate /de-allocate resources on the fly.
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Virgil consists of an open-source encryption library, which implements Cryptographic Message Syntax (CMS) and Elliptic Curve Integrated Encryption Scheme (ECIES) (including RSA schema), a Key Management API, and a cloud-based Key Management Service (Virgil Keys). The Virgil Keys Service consists of a public key service and a private key escrow service.
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SYS-CON Events announced today that Cemware will exhibit at the 19th International Cloud Expo, which will take place on November 1–3, 2016, at the Santa Clara Convention Center in Santa Clara, CA. Use MATLAB functions by just visiting website mathfreeon.com. MATLAB compatible, freely usable, online platform services. As of October 2016, 80,000 users from 180 countries are enjoying our platform service.
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Digitization is driving a fundamental change in society that is transforming the way businesses work with their customers, their supply chains and their people. Digital transformation leverages DevOps best practices, such as Agile Parallel Development, Continuous Delivery and Agile Operations to capitalize on opportunities and create competitive differentiation in the application economy. However, information security has been notably absent from the DevOps movement. Speed doesn't have to negat...
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SYS-CON Events announced today that eCube Systems, the leading provider of modern development tools and best practices for Continuous Integration on OpenVMS, will exhibit at SYS-CON's @DevOpsSummit at Cloud Expo New York, which will take place on June 7-9, 2016, at the Javits Center in New York City, NY. eCube Systems offers a family of middleware products and development tools that maximize return on technology investment by leveraging existing technical equity to meet evolving business needs. ...
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SYS-CON Events announced today that MathFreeOn will exhibit at the 19th International Cloud Expo, which will take place on November 1–3, 2016, at the Santa Clara Convention Center in Santa Clara, CA. MathFreeOn is Software as a Service (SaaS) used in Engineering and Math education. Write scripts and solve math problems online. MathFreeOn provides online courses for beginners or amateurs who have difficulties in writing scripts. In accordance with various mathematical topics, there are more tha...
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Watch My Back is the story of one manas search for courage. Depressed, bullied, intimidated by life and indoctrinated to believe that this was his lot, Geoff Thompson, on the verge of a breakdown, decided to fight back. In a bid to confront his fears, he took a job as a bouncer in one of Britainas roughest nightclubs. Over the next ten years, he was involved in hundreds of brutal and bloody fights that left two of his friends murdered and many more in prison; he turned himself into a fearsome fighting machine. Geoff reached the top of his trade and became addicted to violence. Then it all changed. After nearly being killed in a gang attack, and almost killing one of his attackers, he was forced to reassess his relationship with violence. After writing down his experiences, Geoff discovered a flair for writing. This is the story of an ordinary man who faced his fears and took himself from bedsit to best-seller but very nearly got killed on the way. Geoff Thompson is now the author of over thirty books, a stage play and a BAFTA winning short film.Passing the double divan and the white fitted wardrobes, she headed for the chest of drawers in the master bedroom where two jewellery boxes sat on top of the chest of drawers, her pasty complexion alight with anticipation. She sat on the anbsp;...
|Watch My Back
|Geoff Thompson
|Summersdale Publishers LTD - ROW - 2009-01-05

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Hanting Express (Shanghai Shangnan): Find photos, reviews, information, amenities and compare prices for 2 stars hotels in Shanghai, China.
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This has bugged me for a while and it not been massively important up until i recently started scratchbuilding titan's.
Titan weapons are strange and greatly confusing. I'll start with my pet annoyance first and continue from there:
The Laser Destructor. Its power is immense... 2 5" strength D blasts....
That weird... the hole they are coming out of is slightly larger than an inch on the model, yet the blast is 5 times bigger?
This is what it appears to be doing. Thats some serious physics craziness right there.
"but Richard" i hear you cry " Surely the weapon is spreading out on impact to the ground?"
Bam. Instantly the weapon rules become alot less stupid. Except for one thing. Its a strength D weapon... that means it ignores intervening terrain like small bunkers and broken ferrocrete city ruins!
Surely if ferrocrete doesnt stop the weapons progress, the last beam will travel through topsoil, a thin ferrocrete layer, thin layers of tarmac etc. ?
I was generally inspired by this little fella:
New Rules for laser Destructor:
For each shot the weapon gets, it may do the following:
Select a target for the weapon to fire at and roll to hit. If the hit is successful, mark this point (as if it were a blast template). If its a miss, the point scatters D6" (if a hit is rolled, use the small arrow).
The player may select another point D3+ BS inches away and mark this point. Roll to hit, if successful, mark the point, a miss and it deviates D6" again. Then, run a small blast template down the line between the two. Any models touched by this suffer a hit.
It represents a huge laser beam being directed across a particular target. I figure that it produces, on average, the same kill zone. Its also not just idiotic and "W00T UB3R 3XPL0510N!" like the normal rules.
What you guys think? maybe a bit too complicated?
If anyone believes on telekinesis, raise my hand!!
Sounds fair enough to me, the scatter will make it a very unpredictable weapon, and I always run my opponent by it first to make sure their fine with it, but go for it! I certainly wouldnt have any more of a problem facing this than any other titan weeaponry (Especially the vulcan mega bolter, that thing sucks).
Ask me about the Black and White Space Marine on the Black and White Bike!!!
The weapon in a normal game still scatters 2D6", its just an ordinance blast template.
With this, your ballistic skill matters far more to get an accurate and deadly shot. You could aim to cut through a line of guardmen, chaos tanks or catch a character in the blast. I would think that the most advanced war machines the imperium can use with some of the most deadly weapons wont often fire almost 24 metres (2 metres = about an inch in game) from where you want it (thats rolling 12 and a scatter arrow on 2D6).
This basically represents firing at a target with a beam that lasts for about 2-3 seconds and is generally very powerful. to get the perfect shot, all you need is to roll two 3+'s to hit.
I've been trying to figure out the vulcan mega bolter myself. It seems weird that a weapon that can fire (according to the fluff) 6000 rounds a second can pinpoint each person individualy for a shot. Then again, i have seen footage of targeting systems attached to modern day vulcan cannons that can shoot birds out of the air individually and only waste something like every other shot.
If anyone believes on telekinesis, raise my hand!!
I was always under the impression that rolling 1 die isnt the same as pulling the trigger once. A Marines Automatic bolter at long range with their skill yelds one highly accurate shot whilst eldar shurican catapults are, fluff wise, said to shoot a hail of shurican and yeld 2 dice.
Back to the The Laser Destructor the template might represent several pulse shots clustered around a single taget or indeed a blast so powerful it throws up lot of molten slag and debris essentially. Thats why the thing under the center of the template is borked, its at ground zero, everything else is colateral damage.
Might be true of the shuriken, not of the bolter. Bolter fires single/semi (2) in inquisitor, and can fire 2 in semi auto mode in dark heresy. It fired single/semi in fire warrior as well.
DoW is the only time its not accurately shown.
As we step up into apocalypse, things are supposed to become more fluffy and less "balanced (although since this is happening to both sides, they both are balanced).
Thats why there are rules for firing at a huge horde of nids and running out of ammo.
Also, if the situation you suggested (mutiple laser shots hitting in a general area) is true, the double barreled laser destructor shouldnt shoot 2 shots, the triple barrel shouldnt shoot 3 shots etc.
And why does it scatter 2D6 - 4 and cluster around that area?
The only reason that particular thing is happening is because they saw it like on DoW WA when you activate the titan's lesser weapons and use it to destroy the necrons. And as in the first post, i explained that any weapon that ignores cover wouldn't cause a huge circle of molten slag unless it spread out, in which its range would be severely comprimised.
If anyone believes on telekinesis, raise my hand!!
I really like the idea you put forward for the turbo lazer mate and the W00T UB3R 3XPL0510N! really made me laugh and really makes a lot of sense.
However, how could the same rule be applied to the turbo lazer on the shadowsword as it can't really taget the ground being a fixed turret, although I don't have the exact rules for it to hand but the turbo lazer on the shadowsword is the same as the titan isn't it?
If you try and fail......TRY HARDER
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The volcano cannon is the same type of weapon (for some reason it has a longer range!) so i would say so.
i hadn't noticed, theres a picture in the back of the apocalypse book (pg 190), that has a thunderhawk firing its turbo laser destructor. It leaves a massive groove in the ground and doesnt soak into the ground (which appears to be some sort of rock-crete).
I've been looking at the plasma blastgun. Its two setting are interesting. I think its full setting is the titan equivelent of firing a heavy plasma and the rapid is the equivelent of firing a plasma gun or pistol.
Full fires a direct blob of plasma at its target
Rapid fires some sort of energy streams at its target.
I think its gonna need some rules alterations.
If anyone believes on telekinesis, raise my hand!!


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Cooking for your wife: beta as fuck or living the dream
Worrying about if something makes you a beta means you're a beta
Grow up.
fuck off, anime piece of shit
Do you like French food?
I like French food.
You know you don't earn any points by endlessly starting garbage threads from your phone, right?
I doubt there's anyone who genuinely dislikes French food. Some people think Italian food is better, but even they generally don't hate French food per se.
The only ones that do are people who are under the impression that all French food is nothing more than sheepbrains and duck livers to be quite honest familia.
Steak frites
what are some ways to tell whether a frying pan is good or bad quality?
Look for this logo and don't buy anything without it.
Absolutely not
I'd say you should post an image of the pan you're asking about, but apparently you're incapable of doing so.
Help me /ck/ you're my only hope
I want to eat healthy but I'm just goddamn lazy
I end up eating out all the time even though it's all garbage with few exceptions because the convenience is too tempting. I need a few ideas for quick and easy staple meals I can eat on pretty much a daily basis with minimal preparation (inb4 "cock"); preferably low-sodium and low-carb. While I'm not fat I just generally feel like shit. Day after day I feel low-energy and low-test (I'm not just spouting epic memes when I say that either) and I doubt...
Comment too long. Click here to view the full text.
>Day after day I feel low-energy and low-test (I'm not just spouting epic memes when I say that either)
In that case, go see a doctor. Ask to have your blood analyzed, you may have some thyroid disorder or whatever.
After that, it might help to see a dietician if your insurance covers it. If not, well, try to stick to three meals a day. Personally I have oatmeal and a glass of milk for breakfast, a grilled cheese sandwich and a glass of milk for lunch and a homecooked meal...
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>In that case, go see a doctor. Ask to have your blood analyzed, you may have some thyroid disorder or whatever.
You could be right but I don't have insurance so a doctor is out for the time being.
>read the /fit/ sticky
I'm sure this is in part a lack of exercise problem - I have a pair of dumbbells sitting within arms' reach that I haven't touched in some time and a .txt file wherein I'd written what I ought...
Comment too long. Click here to view the full text.
>That sounds like a good start. I keep buying oatmeal and forgetting I have it for some reason; luckily it keeps as long as it does.
Maybe you could buy them ready made (not sure what they look like in whatever country you're from, this is what it's like in the Netherlands) if you're too lazy to make it yourself. It remains good for about a week or so, but if you eat breakfast every day that's more than enough. Just take a smale bowl of it, add some banana slices,...
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/ck/, /fit/ here. Going to a party with some gymbros, need to bring high protein party dish
Any good recipes you guys got?
I don't think you deserve any, for your OP image.
A bowl of cottage cheese and suck each other off.
Oh wait, that was already the plan, wasn't it?
The host is a grill :'(
Yo , i bought one of these but i want ideas on what else i can do with it except make croissants , any ideas for alternatives preferably not sweet?
Wrap it around pieces of Brie. Bake normally.
well, considering they are already crossants, i dont think theres much you can do about it
roll them up with a little bit of parm, a sprinkle fo garlic, and some cayenne pepper. Instant spicy cheese straws.
I've always wanted to try out vegetarianism for shits and giggles. I figured I'd shoot for a month and longer who knows.
I've eaten so much fucking meat lately I'm starting to get sick of it, so I figure now is a good time to try it out.
I don't eat soy, so what else do you freaks do in place of meat? Literally 98% of the main dishes I know how to make are meat based.
So basically you just want to learn how to cook.
You could have just said that without baiting for replies with veggie shit.
Just eat food minus the meat. Its not fucking rocket science.
Try bean based chilis. Look to asian food as well,as they generally are vegetarian as well.
So, I've started eating coffee beans. Does anyone else on /ck/ do this?
I don't eat that many, just a couple beans per day. I'm tempted to try making chocolate-covered coffee beans, which is why I was curious to try eating whole beans.
I throw a handful in my cereal every morning.
Chocolate covered espresso beans are one of my favorite stacks. I'm not sure I could do it without the chocolate. seems like it would be too dry to swallow.
Eat them while I work (coffee shop). I like adding them to ice cream.
>go for monthly grocery shopping spree
>get a stack of egg roll wraps
>get tons of ingredients for making awesome egg rolls of different styles
>a day later find out I accidentally bought tortilla wraps
I'll be trying to make my own cut I can't find as many recipes online to cross reference as I like. and getting them as nice and thin as they are from the store will take years of experience and Asian blood, neither of which I have....
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I read and replied to your thread.
Biggest misteak of my life.
A carrot slice rolled off the chopping board and into the gap between the bench and the stove. As far as I know it's still there mocking me and laughing at how he escaped the horrors of being eaten and digested. I'll get that little fucker back one day when I accidentally burn the house down.
I was seated next to a man who was telling me about a Freudian Slip. The ticket counter girl apparently had huge knockers. Instead of saying, "I'll have two tickets to Pittsburg," he said, "I'll have two pickets to titsburg."
I could relate. I told him that very morning, I meant to ask for a bowl of cornflakes. Instead, I said, "You ruined me life, you evil bitch!"
What are some good winter comfort foods? I want to get comfy this weekend.
beef stew
For desert, millet pudding prepared with lactose-free milk (for improved taste)
>for main, my man's balls drained onto a plate with a spume of wild homo sperm
What is a very simple recipe to do with long grain rice? I'm just looking for a snack.
just crack an egg in there for simple gainz but don't forget to break the rice in half first so they fit in the pot better desu senpai
a bowl of rice
Hello /ck/,
I need some help. I was reading this recipe (link ahead) and I have doubts about a certain step. English is not my mother language, so I don't fully understand what I have to do.
A visual guide or a good translation to Spanish would be great
The step:
>Fold in the chocolate mixture using a rubber spatula, being careful not to break down the egg whites.
The recipe: http://spanishfood.about.com/od/spanishcustardspuddings/r/Chocolate-Hazelnut-Mousse-Recipe-Mousse-de-Chocolate-y-Avellanas.htm
Folding a mouse is important to preserve the bubbles. Do not stir, gently fold the bottom layer unto the top repeatedly until a uniform texture has been reached.
Ok, I think I got it. I will look more info about that, now that I know what that means.
Fold by gently lifting a part of the batter around and on top of itself. So that you mix by gradual layering instead of any side to side shearing.
I need something to juice and or blend with and something to fry with, maybe a ceramic pan or something I don't even know what I'm looking for, all I know is that I wanna fry chicken and eggplant and drank limonana.
What should I get?
what's your budget
Vitamix and Le Creuset skillet
My budget is willing to spend more for a superior product that'll last me, what's a good model? I'm afraid if I get the cheapest one it'll either, 1. be kinda poo or 2. crap out after some time and I'll have to get another one.
What does /ck/ think of Trader Joes?
They never have any fucking parking and it's always packed.
Otherwise, it's pretty cool.
Good place to get sundries and alcohol, and a shitty place for anything else because it's typically overpriced. Some of the frozen stuff is alright once in a while too.
puff pasty is the cheapest i could find. wine is good. maybe some cheese. other than that i dont bother
What are some food that you could just eat massive amounts of and it wouldn't even be that bad for you? Variety appreciated.
Rice. You can easily eat hundreds of grains.
Pickles probably
Baby carrots
Enjoy your lava hot sodium shits


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After working in Leveraged Finance at RBC Capital Markets and two hedge funds that focused on special situations and distressed investments, Steven Wood, CFA started his own firm focused on generating absolute returns through conventional and unconventional means. GreenWood offers clients primarily long ideas, as well as hedge and short ideas, but gives clients complete control over their own account, and has no lockups or liquidity constraints. GreenWood manages capital for institutions (primarily family offices) and high net worth individuals, but the only requirement is a minimum investment of $10k.
I have worked as a global buyside analyst doing fundamental research for over a decade. Today, I manage a relatively small (sub
I am an activist investor in US and Chinese stocks. I was previously an investment banker in New York Hong Kong and London for 9 years, focused on Equity Capital Markets. I look at both long ideas and short ideas and typically focus on a small number on names where I can spend the time to conduct very deep research. I spend my time living between Los Angeles and Beijing, China.
I graduated from the University of Toronto with a BComm and am now working on developing a partnering business to help get the word out on disruptive investments. I want my research to provide timely and conclusive investment ideas that intend to deliver attractive yields to investors.
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I enjoy following and capitalizing on investing opportunities primarily in the tech and healthcare industry. Most of my free time is spent conducting research on small-to-mid cap equities using a combination of fundamental data analysis and technical studies to come to conclusive results. I manage some money that I invest according to the above technique to ripen my exposure to the financial world.
Previously, I spent some time working for a major commercial construction company where I was responsible for running the back office, occasionally overlooking data and logistics. Most of my time was spent in a financial controller capacity.
I write because I believe in sharing my findings and opinions with those interested so they may come to their own conclusions and in turn spark an intelligent and beneficial discussion.
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Independent healthcare investor.
Work at a top 10 U.S. bank, with focus on capital markets. Personally focused on value investing in the technology space. A lot of folks out there are unwilling to take a hard look at the numbers and do the analysis, so I will do it for them.
I run long-only family office assets with a focus on small-cap deep-value securities. In many cases value is hidden below the surface of simple financial metrics and screenable criteria. I'll publish notes on Seeking Alpha when I have something insightful to share that is not getting proper treatment from mainstream research channels. Please note, I'm very busy trying to relax. Writing articles and responding to emails is an after-hours activity. What I lack in publishing regularity, I hope to offset through quality of dialogue. I have met some ridiculously talented and brilliant analysts through Seeking Alpha. This is my true (selfish) motive for re-engaging in such a public forum.
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I'm a professional poker player with an interest in investing. My investing style is build on the principles of Graham and Dodd: trying to buy companies that are on sale and have a margin of safety in case the investment thesis is wrong. I discuss all my picks at my blog @ alphavulture.com
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Long-only value investor running a fund for accredited investors, and a Marketplace subscription for objective buyside research. Pseudonymous to protect my IR access but if you send me your email address and show me your LinkedIn, I'll show you mine. I'm not like anyone you've ever met and am not doing this for the traditional reasons. I'm always up for a conversation with anyone interested in value investing or mental models.
My Marketplace subscription service, called "Outsourced Analyst," provides small-mid-sized funds, family offices, or high-net-worth investors the workflow of an analyst for a hundredth of the price. I write objective coverage of high-quality, underfollowed small-caps that I'm working on / following. Subscribers also have early (sometimes exclusive) access to writeups of some of my best ideas like those I've posted on LQDT, CRAI, FC, LGIH, BOOM, CSWI, and so on. Bonus material is thought pieces - I place a lot of emphasis on learning and getting better - so if/when I make mistakes, I'll write up postmortems with what I learned, and maybe they'll help you as well... Membership will be limited to the first 250 subscribers.
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Mr. Winston received an MBA in Finance and Economics from Columbia Business School in 2005, and a BA in Economics from Cornell University in 1999. At Columbia he completed the school's program in Value Investing, and while at Cornell studied for a year at the London School of Economics. He began his career in 1999 with Credit Suisse First Boston.
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In the midst of your Thanksgiving chaos, you're probably in need of a few laughs. As you read yesterday, the Butterball turkey hotline has had its share of weird Thanksgiving questions. The crazy things that people ask will never cease to amaze me.
After the jump, see some more of the funniest (yet real) Thanksgiving questions that the Butterball turkey hotline has ever received. Not sure they'll make your day any less stressful if you're the one cooking, but they will make you chuckle.
So here they are, some more of the strangest things ever asked on the Butterball turkey hotline, courtesy of Reuters:
Is it okay to thaw my turkey in the bathtub while bathing my kids?
Can I brine my turkey in the washing machine?
Can I use my oven's self-cleaning cycle to speed up the cooking process?
If I cut my turkey with a chainsaw will the oil affect the taste?
Can I take my frozen turkey into my sauna to thaw it faster?
Get all your Thanksgiving questions answered by the Butterball turkey hotline at 1-800-BUTTERBALL. HAPPY THANKSGIVING!
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- Tweets that mention Thanksgiving 2010: More Butterball Turkey Hotline Tales | The Wire | SheKnows.com Blog -- Topsy.com


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Wireless Tv Headphones
MyWirelessTV Wireless HDMI --need to check this out. Really want to mount TV on fireplace stone wall but don't want a major reno in order to accomplish.
MyZone Wireless TV HEADPHONES NEW As Seen On TV Product Gift TELEBrands
Coolest Speakers........ Black Diamond 3 Bluetooth Wireless Speaker
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Apple Airpods - Wireless Headphones
Wireless earbuds that will play your music, answer your phone and they are waterproof (you can wear them while you swim). Need! (ok, Want!).
Cat ear headphones = purrfection!
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Beats by Dre Beats Powerbeats 2 Wireless In-Ear Headphones - Assorted Colors - White


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Digital download price compare
We haven't found any digital download available at this moment.
Average quiz game
It is a puzzle game that is based on a game show that use to run on the television with the same name. The game involves 5 families whose members have been divided in teams to play the quiz and other competitions in the game. The teams will have to match answers to the question that are based on research or polls. The points that will be earned by a family is dependent on the number of the members that respond correctly to the questions. The game also involves helping each other out and is very interesting in terms of the questions that are there. The game has a very good interface that enables users to get a grip on the gameplay very easily and makes them understand all the aspects of the gameplay. The graphics are also very good and so are the level designs which give you a distinct chunk of questions and some very inviting puzzles. You also have to be quick in answering the questions because they are based on time. The team with the highest points will ultimately win the game. The game is overall average but fun for trivia fan lovers.
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All New Family Feud (pc game)
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OSCE PA seeks to achieve serious progress in Karabakh settlement
April 17, 2014 - 15:53 AMT
PanARMENIAN.Net - OSCE Parliamentary Assembly seeks to promote the peaceful settlement of the Nagorno Karabakh conflict, OSCE PA President, Speaker of the Montenegrin Parliament, Ranko Krivokapic said, according to Armenia Today.
As he told journalists in Baku, OSCE supports solutions offered by the Lisbon and Madrid Principles for the conflict settlement, as well as UN Security Council resolutions.
"We are currently working with the OSCE chair to achieve progress in the Minsk Group mediation activities. We aim to achieve serious results as recent developments in Ukraine prove the necessity of solving the unsettled conflicts," Krivokapic said.
The conflict between Nagorno Karabakh and Azerbaijan
The conflict between Nagorno Karabakh and Azerbaijan broke out as result of the ethnic cleansing launched by the Azeri authorities in the final years of the Soviet Union. The Karabakh War was fought from 1991 (when the Nagorno Karabakh Republic was proclaimed) to 1994 (when a ceasefire was sealed by Armenia, NKR and Azerbaijan). Most of Nagorno Karabakh and a security zone consisting of 7 regions are now under control of NKR defense army. Armenia and Azerbaijan are holding peace talks mediated by the OSCE Minsk Group up till now.
Top stories
Levon Yolyan proposed signing a purchase and sale agreement, enabling the acquisition and distribution of gas by the South Caucasus country.
Karabakh Defense Army troops continue controlling the situation along the entire line of contact, ready to thwart any sabotage.
Do you all agree that she should embrace the position she took as Senator and candidate, even though she did not take it as SecState?
Armenia's international tourism expenditures amount to $502 million, while international tourism receipts totaled $458 million.
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Samsung Galaxy S8 "has been delayed by the flaming Note 7" The Samsung Galaxy S8 has been tipped to boast everything from a new, revamped design to a faster processor.
Nico, 1960s cult singer with Velvet Underground gets biopic treatment The 1960s cult pop culture figure will be played by Denmark's Trine Dyrholm, winner of this year's Berlin Silver Bear for best actress.
EU4Business Week: Europe seeks to support Armenia-based SMEs Implemented by EBRD, the workshop on SME finance on October 26 focuses on instruments of creating value for small businesses.


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Today's Devotional
Fireworks and MercyHow does it make you feel to know that God understands your human frailty and has mercy on you?
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Cloning: Past, Present, and the Exciting Future
- Kristopher Williamson
- 1 years ago
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1 Cloning: Past, Present, and the Exciting Future by Marie A. Di Berardino, Ph.D. The not-too-distant future Jimmy walks into the neighborhood pharmacy to fill his prescription for a protein he was born without. He lacks the gene for blood clotting factor IX and relies on the local drugstore for his medicine. Jimmy pulls open the bag that contains his 90-day supply of patches, removes the old patch from his chest, and attaches a new one. He adjusts his jersey and heads out to meet his buddies for a game of touch football. Even though he is hemophiliac, Jimmy isn t worried about the bruises and scrapes he is sure to get. Similarly, Ø Christine is scheduled to have her own genetically reprogrammed skin cells transplanted to replace her severely damaged heart cells. Ø Margo, who has Parkinson s disease, receives special nerve cells. She is not concerned about tissue incompatibility and rejection, because these cells are her own genetically reprogrammed skin cells. Ø Patients routinely buy anti-cancer or anti-viral drugs in large quantities to treat their conditions. This is the future. It is what Dolly so wondrously has wrought. Born July 1996, she is the first mammal successfully cloned from an adult cell, one taken from a ewe s mammary gland. Nuclear transfer Dolly was not created in the ordinary way. Typically, a lamb is the product of natural reproduction two germ cells, a sperm from an adult male and an egg (oocyte) from an adult female, fuse at fertilization. Each of these germ cells (the sperm and the oocyte) contributes half the chromosomes needed to create a new individual. Chromosomes are found in the cell s nucleus and they carry the DNA, which is the genetic blueprint for an individual. The process that produced Dolly differs from ordinary reproduction in two major ways. First, body (or somatic) cells from an adult ewe s udder (this is the donor) were placed in a culture dish and allowed to grow. The nutrients were then removed from the culture, which stopped the cells growth. One of these nongrowing cells was then fused (by electric jolts) with another ewe s oocyte from which the nucleus had been previously removed (i.e., Federation of American Societies for Experimental Biology 1 Office of Public Affairs
2 enucleated, so it had no chromosomes). This procedure is known as somatic cell nuclear transfer. Within a day the fused cells began to divide in the culture dish. After several divisions, the early embryo was transferred to the uterus of a surrogate mother and allowed to develop. Second, unlike the sperm and the egg, each of which contributes half the number of chromosomes at fertilization, each body cell contains twice the number of chromosomes in each germ cell. So fusion of a sperm and an egg forms an individual whose full genetic composition is unique to that individual. On the other hand, the embryo cloned from somatic cell nuclear transfer begins development with the diploid (double) number of chromosomes, all derived from one somatic cell (adult udder) of a single individual. This embryo has the same nuclear genetic composition as the donor of the somatic cell. In the end, three sheep contributed to the production of a single lamb clone: a Finn Dorset sheep donated her udder cells for culture; a Scottish Blackface sheep donated the enucleated oocyte (with its nucleus removed, thus losing its own genetic identity in the process); and a Scottish Blackface sheep became the surrogate Timeline This illustrated timeline shows essential milestones in basic research that led to the cloning of Dolly and beyond, and some benefits derived from that research. Some of the initial hypotheses have been refuted by later studies. 1880s Wilhelm Roux and August Weismann independently propose the germ plasm theory: The egg and sperm contribute chromosomes equally to the zygote (fertilized egg). The chromosomes are carriers of the hereditary potentials, and the germ cells (gametes) of the embryo are the only ones to carry the complete set of hereditary potentials (nuclear determinants), whereas each somatic (body) cell type contains only part of these potentials required for the specific cell type Wilhelm Roux performs the first test of the germ plasm theory: Destruction of one cell of the 2-cell frog embryo with a hot needle results in development of a half-embryo, supporting mistakenly the Roux-Weismann theory Hans Dreisch isolates blastomeres of 2- and 4-cell embryos of sea urchins and observes development of blastomeres into small but complete larvae. These and other experiments disprove the Weismann-Roux theory Hans Spemann splits a 2-cell newt embryo into two parts, successfully producing two larvae. Breakthroughs in Bioscience 2
3 mother, carrying the embryo to birth. The clone (Dolly) was easily identified because she had the physical traits of the Finn Dorset sheep that donated the udder cells and differed from the traits of the Scottish Blackface sheep used as the surrogate mother and the oocyte donor. And now Dolly herself is a mother the old-fashioned way by mating with David, a Welsh mountain ram, and giving birth to Bonnie. In fact, Bonnie now has other siblings. Can Dolly help Jimmy, Christine, and Margo? Imagine herds of female sheep, cattle, and goats producing large quantities of human proteins in their milk, an ideal place for those proteins to be harvested and used to treat patients like Jimmy, the hemophiliac, whose blood cannot clot. We can realize this dream today one step at a time, because the process that produced Dolly also can be used to produce the transgenic (one species carrying another species genes) clones. Scottish scientists first removed cells from a fetal lamb and grew them in a culture dish. Multiple copies of fragments of DNA (deoxyribonucleic acid, which holds genetic information) containing the human gene for blood clotting factor IX were added to the dish and coaxed into the cells. Some cells incorporated the human DNA into their chromosomes, thus becoming transgenic cells, or cells containing a transferred gene. These transgenic cells were then separated from those without human DNA and used to create Polly, the transgenic sheep that today produces the human clotting factor IX in her milk. Purposely, scientists genetically designed the transgenic sheep clones so that the human gene would function only in the mammary gland. It will soon be possible for the human clotting factor IX protein to be routinely harvested and purified from the sheep milk. Obviously, researchers still need to conduct controlled clinical studies before this protein is available for hemophiliacs like Jimmy, but they have already made an astonishing breakthrough. Transgenic clones The importance of the transgenic clones is that biotechnology is now being extended to produce different human proteins like insulin (diabetes), interferon (viral infections), clotting 1914 Spermann conducts early nuclear transfer. By using a strand of baby hair, he partially constricts a newly fertilized egg cell (zygote), forcing the nucleus to one side and only cytoplasm to the other. As the nucleus side of the cell divides successively to the16-cell stage, a nucleus slips over to the cytoplasm on the other side. Cell division starts on this side also, and the hair knot is tightened, preventing further nuclear transfer. Twin larvae develop, one slightly older (the side with the initial nucleus) than the other (the side with the initial cytoplasm). This proved that the nucleus from a 16-cell stage could direct the growth of another larva. Federation of American Societies for Experimental Biology 3 Office of Public Affairs
4 factor VIII (hemophilia), and tissue plasminogen activator (dissolving blood clots). In other words, female clones of such animals as cattle, sheep, and goats are being genetically designed to be dairy/pharmaceutical producers, a virtual living biopharmaceutical industry. Transgenic clones of mammals are a major advance in biotechnology because they can synthesize, in large quantities, complex molecules critically This cloning process simply accelerates the older, slower, and less predictable methods of crossbreeding and hybridization. Strictly speaking, clones obtained from nuclear transfer are not exact copies of the donor. A clone like Dolly produced by oocyte-somatic cell fusion is a mosaic a mixture of the oocyte and body cell. In addition to the nucleus, the donor cell contributes a tiny amount of cytoplasm and We are only beginning to understand the molecular changes involved in nuclear reprogramming, yet this line of basic research may result in some of the most beneficial applications to humans. It might permit us to de-differentiate mature cells and re-differentiate them into specific cell types required for tissue repair. required for patient care. The current recombinant DNA technology in bacteria is capable of synthesizing only simple proteins, but not the complex molecules that sheep can produce in large quantities in their milk. Indeed, Jimmy will be able to wear his patch very soon. While these advances are on the horizon for us, beneficial applications to agriculture are already being implemented. Transgenic cloning can be used for the genetic improvement of livestock related to milk production, quality of meat, growth rate, reproduction, nutrition, behavioral traits, and/or resistance to diseases. cell membrane to the oocyte, but the egg cell contributes an enormous amount of cytoplasm and cell membrane to the fused product. In fact, virtually all mitochondria (the organelles in the cytoplasm that are the major source of cell energy and contain DNA of their own) are derived from the oocyte cytoplasm. So cloning of multicellular organisms is not equal to true cellular cloning that results from asexual reproduction, when a one-celled organism like the amoeba divides and clones itself. Similarly, plants clone themselves when they reproduce by budding or sprouting new shoots. The agricul- tural industry has intentionally propagated by cloning bananas, grapes, apples, sugar cane, pineapples, potatoes, asparagus, and many other plants. Identical twins and triplets that occur among many multicellular animal species including humans, are derived by a cloning process. A cell, isolated from other cells growing in a culture dish, gives rise after cell division to a clone. All these examples include the exact duplication of the whole body cell, including the nucleus, cytoplasm, and cell membrane. Why is this such a big deal? Dolly has debunked a long-held, generally accepted biological concept: Adult cells have their fate sealed; put another way, once an udder cell, always an udder cell. This means that the genetic status of the adult donor nucleus had to be reprogrammed, and that the oocyte cytoplasm seems to contain the appropriate molecules to trigger this reprogramming. The value of cloning from an adult cell is that we can predict that the clone will be very much like the donor animal, whereas we cannot do so when the clone is derived from an embryonic or fetal cell. Even when the parents of embryos and fetuses are known, we cannot yet foretell exactly the physical traits of their offspring; they are a combination of traits from both parents. Additional variations occur 1940s 1950s Various species of mammalian embryos are cloned by embryo splitting, but success is limited to splitting of embryos at stages prior to implantation to the uterus Robert Briggs and Thomas J. King transplant a nucleus from a frog embryo somatic cell into an unfertilized, enucleated oocyte. These injected eggs developed into tadpoles and many metamorphosed into juvenile frogs. This technique, nuclear transplantation (nuclear transfer) becomes the prototype experiment for cloning multicellular organisms. Breakthroughs in Bioscience John B. Gurdon and Robert G. McKinnell, by using the nuclear transfer method on different species of frog (Xenopus and Rana, respectively), obtain adult frogs that produced normal progeny. This demonstrates totipotency of embryonic nuclei Robert G. McKinnell, Thomas J. King, and Marie A. Di Berardino obtain swimming larvae from enucleated oocytes injected with adult frog kidney carcinoma cell nuclei. This demonstrates that some cancer cells can be controlled by the differentiation process, and that inducing cancer cells to differentiate may stop the cancer process.
5 because, during the maturation of the parents sperm and egg before fertilization, the genes are randomly distributed to sperm and egg cells. Thus, offspring cloned from adult cells are more similar to their donor than are offspring that result from sexual reproduction, i.e., a combination of sperm and egg. There is, however, no guarantee that an exact copy will evolve, because variations in the physical traits of the donor may result from environmental influences during pre- and postnatal development and, in some cases, from the mitochondrial DNA of the oocyte. Totipotency Animal cloning began more than a century ago when scientists wondered how an initially formless mass of cells, the blastocyst, develops into a structured organism composed of separate skin cells, blood cells, muscle cells, brain cells, stomach cells, and so on. They specifically wanted to know whether these many different cell types were irreversible or whether these differentiated cells could reverse and express again the totipotency of the early embryo. Early Embryonic Cells Are Totipotent By using an early version of the nuclear transfer technique in their initial studies, scientists established that nuclei from early embryonic cells were indeed totipotent; that is, they could develop into complete fertile organisms, with all the cell types and organs of an adult. These studies showed that frogs, salamanders, insects, fish, and mammals (mice, rats, rabbits, pigs, goats, cattle, and sheep) could be produced by this process. Furthermore, many of these species were fertile. (The only primates to have been cloned by nuclear transfer thus far are two Rhesus monkeys produced from the nuclei of an eight-cell stage embryo.) Few Nuclei Remain Totipotent throughout Development Scientists now know that the older the donor cell from which the nucleus is transplanted, the more likely it is that the injected oocyte will develop abnormally. In fact, only a few tadpole nuclei from frogs have been shown to be totipotent, and those may have come from immature cells. When fully differentiated cell types (pigment, skin, red and white blood cells) from adult frogs were used as donors, their nuclei gave rise to tadpoles but not to fertile frogs. In mammals, very few fetal cell nuclei (approximately 0.3-2%) from sheep and cattle led to the normal development of living newborn animals. Dolly, produced from an adult cell, represents only a 0.2% cloning success. The inability to clone most nuclei from advanced stages probably results from protein changes that occurr in the chromosomes during cell differentiation, changes that will have to be identified and controlled in the future. Genetic reprogramming Scientists found that cloning actually reprograms nuclear function and returns some nuclei to an undifferentiated state. This discovery followed a line of basic research that may result in some of the most beneficial applications to humans. Soon after a nucleus is transferred to an egg, there occurs a two-way transfer of specific proteins between the nucleus and cytoplasm. This transfer causes modifications in chromosomal proteins, resulting in 1964 F.C. Steward grows a complete carrot plant from fully differentiated carrot root cell. This and the previous amphibian experiments led some scientists to believe that cloning from differentiated animal cells was possible John B. Gurdon and V. Uehlinger succeed in growing adult frogs from injection of tadpole intestinal cell nuclei into enucleated oocytes. 1970s By using nuclear transfer, researchers obtain pre-feeding larvae from nuclei of differentiated frog skin (J.B. Gurdon, R.A. Laskey, O.R. Reeves) and lymphocytes (M.R.Wabl, R.B. Brun, and L. Du Pasquier) James McGrath and Davor Solter develop nuclear transfer technology for mammalian embryos. Fertile mice were obtained when a nucleus surrounded by minute amount of cytoplasm from a 1-stage fertilized egg was fused with another enucleated fertilized egg. Federation of American Societies for Experimental Biology 5 Office of Public Affairs
6 nuclear DNA synthesis, and later in the expression of a new set of genes. These and other changes reflect reprogramming of nuclear function by chemicals in the oocyte cytoplasm. When investigators identify the role of these proteins, we will understand how to convert a nucleus from a differentiated adult cell type into one that is relatively immature. This might permit us to actually reprogram mature cells and turn them into specific cell types required for tissue repair. By doing this, we could potentially replace damaged heart or brain cells by reprogramming the patients own skin cells into heart muscle cells and nerve cells (for people like Christine and Margo). Dolly is only the first! Dolly is no longer unique, although she always will be the first animal cloned from an adult cell. Being the result of a nuclear transfer between an adult ewe udder cell and an enucleated oocyte, she showed that an adult mammalian cell could be reprogrammed genetically to be totipotent and give rise to an entire individual that grew to maturity and gave birth to offspring of her own. Soon after Dolly was born, a set of triplet transgenic calves bearing a foreign gene was cloned from fetal cells, all sharing the same nuclear genes. This indicates the feasibility of producing herds of sheep and cattle capable of producing the same protein. In Hawaii, 17 months after the announcement of Dolly s birth, 32 mice were cloned from cumulus cells (i.e., cells that surround the developing oocytes in the ovaries), confirming successful cloning from adult cells. Ten of these clones have themselves produced normal progeny, and some of the 31 clones themselves came from clones, demonstrating the feasibility of augmenting the number of clones. In December 1998, eight calves were cloned from a single adult cow s cumulus and oviductal cells (cells lining the tubes of the oviduct that transport oocytes to the uterus). These studies showed that not only adult cells could be reprogrammed, but also that some differentiated cells (such as cumulus and oviductal cells) had the same capability. Keep in mind that not all adult cells are differentiated; for example, undifferentiated cells are found in bone marrow, the intestinal lining, etc., of adults. Direct advantages Transgenic clones can be directly beneficial to humans, other animals, and agriculture in additional ways. Ø They may be developed for tissue and organ transplantation. Although not yet a reality, there is promise that large animals can be genetically designed and cloned so that their tissues and organs will not trigger immunological responses in the recipient and cause them to be rejected. Recently, muscle rigidity and tremors in parkinsonian rats were improved by transplanting cloned transgenic bovine neurons into their brains. This research, called xenotransplantation, is one of the many avenues being pursued in an attempt to alleviate the desperate shortage of human tissues for transplantation. Ø Domestic animals can be genetically designed to express a certain human disease and therefore serve as models for the study and treatment of human illnesses. Although many mouse models of human diseases are available today, such models in large domestic animals physiologically more similar to humans are sparse and critically needed. Ø Somatic cell nuclear transfer might help preserve endangered species such as pandas that have low reproductive rates. Indirect advantages Two other significant gains from clones are worth mentioning. First, inducing cancer cells to differentiate is a useful type of therapy. We know that many types of cancer cells are less specialized than their normal counterparts. For this reason investigators suspected that the precursors of cancer cells could be immature cells or stem cells that fail to complete differentiation. If this is Marie A. Di Berardino, Nancy H. Orr, and Robert McKinnell obtain pre-feeding and feeding tadpoles from transplanting nuclei of adult frog erythrocytes (unlike humans, frog mature red blood cells contain nuclei) Steen Willadsen clones lambs by fusing a nucleus of an 8-cell embryo to enucleated oocyte. An electrical impulse usually was used for the oocyte fusion and activation process. Other researchers subsequently succeeded in producing full-term cattle, sheep, pigs, goats, and rats by using a similar approach, as long as the blastomere nuclei were obtained from embryos prior to implantation. Breakthroughs in Bioscience 6
7 so, then by using information gained from nuclear transfer technology, we may be able to induce the cells to mature and stop making tumors. Previous studies have demonstrated that we can control at least some cancer cells by using the differentiation process. Second, aged cell nuclei can be rejuvenated. People and other organisms change as they age. Environmental insults and diseases cause these If we could isolate these substances, we might be able to alleviate or reverse senescence. chromosomes and others with abnormal ones. So far, the prospects for identifying an abnormal embryo prior to transfer to the uterus are poor. And there are other scientific concerns: Ø the life span of the clone is unknown, as is Ø the compatibility between the genetic products in the cytoplasm of the oocyte and the donor cell, and Ø during the normal process of sexual reproduction, there is a natural selection of the fittest germ cells in fertilization. Although this process is not perfect (i.e., it fails to eliminate some harmful mutations), it does not exist in cloning. So we can see that it is unlikely that cloning of a human being from any donor age will happen any time soon. Indeed, the scientific community was so strongly opposed to the production of a human being by cloning techniques that the Federation of American Societies for Experimental Biology and other professional organizations representing more than 67,000 scientists have issued a voluntary moratorium against such an act. The groups endorsing this position included those scientists most capable of performing this type of work. Although these scientists believe that cloning a human being is unethical and reprehensible, they are still concerned that some of the antichanges; others are intrinsic to the organism. Studies using cell culture have shown that body cells grow and divide normally in culture for awhile, but eventually stop dividing, become senescent, and die. An exception was seen in aged frog red blood cell nuclei (human red blood cells lack nuclei): After their transfer into enucleated oocytes, frog red blood cell nuclei were rejuvenated. They carried out the formation of tadpoles that survived almost a third of the way to metamorphosis. The oocyte cytoplasm contains an abundance of chemicals that promote DNA synthesis and cell division after normal fertilization. We believe that these substances also rejuvenate aged cell nuclei and turn non-cycling frog red blood cells into 1993 M. Sims and N.L. First report for the first time production of calves by transfer of nuclei from cultured embryonic cells. active ones. If we could isolate these substances, we might be able to alleviate or reverse senescence. Perils Although Dolly, mice, and calves have been the first animals cloned from adult cells, the low efficiency in producing them negates attempts to clone humans. The Dolly experiment began with 434 attempts to fuse a mammary gland cell to an oocyte, and ended with only a 0.2% success rate; the remaining attempts resulted in death either during fusion or in various developmental stages. Moreover, the 1-2% success rate with mouse and 1-5% with calf cloning from adult cells are equally low. And high frequency of fetal (approximately 60%) and neonatal (approximately 50%) deaths are common. In a real sense, cloning is a roulette game. Even if cloning from adult cells did become efficient, there still would be serious hazards. Ø Donor cells could suffer mutations from radiation, chemicals, aging, and/or errors in DNA replication during the lifetime of the donor, which would be transferred to the clone. Ø Mutations could arise in donor cells during cell culture, not an unusual event, and there is no way of distinguishing a normal donor cell from a mutant one. Ø The embryo may be a mosaic of cells, some with apparently normal 1997 Dolly, the first mammal cloned from an adult cell, is produced in Scotland. Ian Wilmut and colleagues report the birth of a lamb derived from the transfer of a nucleus obtained from an adult ewe udder cell into an enucleated oocyte that was then implanted into a surrogate mother. Primate cloning: Two Rhesus monkeys are the first and only primates thus far cloned by nuclear transfer from the 8-cell stage Don Wolf s laboratory at Oregon Regional Primate Research Center The Honolulu group, led by Teruhiko Wakayama, reported the production of a large number of live mice from injecting nuclei taken from adult ovarian cells into enucleated oocytes. These investigators also report success in recloning efficiently the first clones. The Japanese group, led by Yoko Kato, produced eight calves from cumulus and oviductal cells derived from one adult donor. Federation of American Societies for Experimental Biology 7 Office of Public Affairs
8 cloning legislation designed to prevent the cloning of a human being contains language that also will prohibit vital biomedical research that can lead to the repair of diseased and damaged human tissues and organs, and to possible treatments and cures for diabetes, cancer, Parkinson s disease, and other neurodegenerative diseases. Other nations have found a successful balance between these two concerns. Many European countries have outlawed attempts to clone humans, while preserving the freedom of scientists pursuing cloning studies in non-human organisms because of the potential benefits. In the United States, the National Bioethics Advisory Committee recommended an imposed period of time in which no attempt is made to create a child using somatic cell nuclear transfer. In their 1997 statement, the committee cautioned, Any regulatory or legislative actions undertaken to effect the foregoing prohibition on creating a child by somatic cell nuclear transfer should be carefully written so as not to interfere with other important areas of scientific research. Knowledge: threat or promise? When scientists first discovered anesthesia, atomic energy, and recombinant DNA, we did not know if these breakthroughs might lead to deleterious applications. The choices we make for the application of knowledge reside in ethical decisions by humans. Animal cloning, like other research, was initiated to seek fundamental knowledge for the benefit of humankind. In addition to expanding the knowledge base in cellular, developmental, and molecular biology, as well as in cancer and aging, cloning has now been applied to enhance medicine and agriculture. Presently, hospital committees in the United States bar attempts to clone humans because of clinical, safety, and ethical concerns. Cloning is only one of many discoveries in which society will have to choose which applications are ethical and which ones are not. Suggested Readings For the original report on Dolly, see I. Wilmut et al. (1997) Viable Offspring Derived from Fetal and Adult Mam-malian Cells. Nature, 385: For a general treatment of cloning, see I. Wilmut (1998) Cloning for Medicine. Scientific American, December For the original report on transgenic lamb clones, see A. E. Schnieke et al. (1997) Human Factor IX Transgenic Sheep Produced by Transfer of Nuclei from Transfected Fetal Fibroblasts. Science, 278: For the original report on transgenic calf clones, see J. B. Cibelli et al. (1998) Cloned Transgenic Calves Produced from Nonquiescent Fetal Fibroblasts. Science, 280: For the original report on cloning mice from adult cells, see T. Wakayama, A.C.F. Perry, M. Zuccotti, K.R. Johnson and R. Yanagimachi (1998). Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature, 394: For the original report on cloning from adult cow cells, see Kato, Y., Tani, T., Sotomaru, Y., Kurokawa, K., Kato, J., Doguchi, H., Yasue, H., and Tsunoda, Y. (1998) Eight calves cloned from somatic cells of a single adult. Science, 282: For the original article on treating parkinsonian rats, see W. M. Zawada et al. (1998) Somatic cell cloned transgenic bovine neurons for transplantation in parkinsonian rats. Nature Medicine, 4: For original articles on cloning cancer cell nuclei, see T. J. King and M. A. Di Berardino (1965) Transplantation of nuclei from the frog renal adenocarcinoma. I. Development of tumor nuclear-transplant embryos. Ann.N.Y.Acad.Sci. 126: and R.G. McKinnell, B.A. Deggins and D.D. Labat (1969). Transplantation of pluripotential nuclei from triploid frog tumors. Science 163: For an article on cloning red blood cell nuclei, see M.A. Di Berardino and N. Hoffner Orr (1992) Genomic potential of erythroid and leukocytic cells of Rana pipiens analyzed by nuclear transfer into diplotene and maturing oocytes. Differentiation 50:1-13. For a thorough coverage of the science and history of animal cloning, see Marie A. Di Berardino (1997) Genomic Potential of Differentiated Cells, Columbia University Press, N.Y. Marie A. Di Berardino, Ph.D., is Professor Emerita of Biochemistry, The Medical College of Pennsylvania-Hahnemann School of Medicine, MCP Hahnemann University, 2900 Queen Lane, Philadelphia, PA She has conducted research on the developmental potential of differentiated frog cells and cancer, and has lectured and published widely on this subject. Science editor for this article was Ida Chow, Ph.D., a natural clone herself (her identical twin sister is a physicist living in Brazil). She is the Executive Officer of the Society for Developmental Biology. Her research is on cell-cell interaction during vertebrate development. Breakthroughs in Bioscience 8
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Enbridge Inc.
- Enbridge announces job cuts in Houston, elsewherebizjournals.com
- Enbridge Layoffs Will Affect 370 In Canada, 160 In The U.S.huffingtonpost.ca
- Enbridge Inc cuts 530 jobs, 5 per cent of work force in Canada, U.S.business.financialpost.com
- Enbridge cuts 5% of workforce, including 370 jobs in CanadaCBC
- Enbridge to reduce staff by 5%The Globe and Mail
- TransCanada officially calls for new shipments on its mainline with lower than expected tollsbusiness.financialpost.comNiagara Falls
- Canadian oil pipelines resume operations after activists halt flow in five U.S. locationsbusiness.financialpost.comGulf Coast
- Dakota Access pipeline work resumes near site of huge, months-long protestLA Times
- Pro-oil First Nations seek end to pipeline gridlockbusiness.financialpost.comFort Mcmurray
- LEnbridge to sell Southeast Sask. pipeline assets to Tundra for $1Bleaderpost.comManitoba
- Royal Bank of Canada CEO touts pipelines as key to transitioning to a greener economybusiness.financialpost.comMontreal
- How a retired university professor outperforms the marketThe Globe and Mail
- Natural resources minister rejects idea First Nations united against pipelinesCBC
- U.S., Canada aboriginal tribes form alliance to stop oil pipelinesthenews.mx
- First Nations coalition vows to resist increased transport of crude through territoriesThe Globe and MailNorth Dakota
- Spectra Energy says rapid corrosion, other factors led to Salem pipeline explosiontriblive.comNorth America
- North Dakota governor calls in National Guard ahead of pipeline rulingbusiness.financialpost.comWashington
- Ontario Energy Board reviewing Enbridge-Spectra dealThe Globe and Mail
- 'We've stifled all of them': Canadian energy companies look south of the border for growth amid gloomy prospects at homebusiness.financialpost.com
- The irony of Enbridge and Bay Street's new love of diversificationThe Globe and Mail
Frequent names
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Год издания: 2005
Количество страниц: 304
В продаже с 18.01.2012
Как только книга It's One O'clock and Here is Mary Margaret Mcbride: a Radio Biography станет доступна для заказа в одном из интернет-магазинов, Вам на e-mail будет отправлено уведомление.Укажите e-mail для связи:
Book Description "This discerning biography of radio pioneer Mary Margaret McBride illuminates an entire cultural era and offers fascinating parallels to our own time. In Susan Ware's engaging narrative, McBride emerges as an icon of twentieth century popular culture and its romance with what we now describe as 'talk radio.' McBride's story is a tale of power, freedom and connection boldly interpreted by a leading woman's historian." ?Joyce Antler, author of The Journey Home: How Jewish Women Shaped Modern America "Well written and lively, Susan Ware's biography rightly restores McBride to her proper place in broadcasting history." ?Susan Douglas, author of Listening In: Radio and the American Imagination One of the most beloved radio show hosts of the 1940s and 1950s, Mary Margaret McBride (1899?1976) regularly attracted between six and eight million listeners to her daily one o'clock broadcast. During her twenty years on the air she...


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5 Tips and Tools For Convenient Holiday Travel
The 2011 holiday travel season is almost upon us. Whether you're visiting family a few hours away or ringing in the New Year with a romantic European getaway, chances are your travels could use another layer of convenience. Here are five travel-related tips and tools to help you make the most of your holiday getaway.
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The last couple of years have seen the meteoric rise of deal sites such as Groupon and Gilt Groupe. But if you thought deal sites only offered sales on luxury handbags and rooftop cocktails, think again. Tablet, Vacationist and Jetsetter offer savvy travelers great rates on hotels and vacation packages to destinations all around the world. For more deals, check out Mashable's list of best travel sale sites.
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Mobile devices are quickly becoming a traveler's best friend. Apps such as Tripit and Expensify can help you track your itineraries and expenses in one place. GateGuru provides maps and tips for more than 80 airports, so you always know how long it takes to get through security (and where to get a pre-flight latte). If you think you'll be using these applications – or your cell phone in general – outside the U.S., make sure you know whether your cell phone will have coverage overseas .
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Some people view frequent-flyer miles as a means to an end. For others, miles are a religion. If you fall into the latter category, visit WebFlyer for an exhaustive collection of details on frequent-flyer programs. Whether you're looking to compare blackout dates or want tips on where to buy, trade or sell your airline miles, WebFlyer may be worth a bookmark in your browser's travel folder.
4. Take the Train
Flying is usually the obvious choice for getting to Asia or Europe from the U.S. But what about when you're already overseas, and you want to travel while you're there? Forbes recommends resisting the urge to rent a car or buy another plane ticket, and to take the train instead. More travelers are finding trains to be the best option for getting around foreign lands – they're often less expensive than other modes of transport, they can be nearly as fast and they make for great scenic tours of the spaces between populous areas.
5. Lodging Alternatives
While there's nothing quite like a stay in a luxury hotel, sometimes your travels can benefit from the trappings of home, and a new wave of sites offer such homespun lodging alternatives. Sites like Airbnb, VRBO and HomeAway let vacationers rent private homes and apartments for their vacation lodging. These sites are sure to have appealing options, whether you're looking for a Hawaiian beach house big enough for your entire brood, or a chic urban penthouse for Thanksgiving in New York City.
Are you planning a trip during the holiday season? What travel tools or tips do you rely on?
Links to non-Ally websitesWe provide links to third-party websites for your convenience. Although we provide a link, Ally Financial is not responsible, nor can we guarantee their products, services, or information. We suggest you review their online policy and security practices to learn about this third party and how they handle consumer information.


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Romantic with an edge, feminine and strong, Grace in LA jeans feature specialized pocket treatments with intricate embroidered, bejeweled, and metallic motifs.
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Since 1972, The Fort has been offering a huge selection of western wear and western decor at everyday low prices including cowboy hats, work wear, cowboy boots ...
Jeans for Women - Grace in LA | Buckle
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Shop Grace in LA Jeans - Women's Grace Jeans | Free ...
When you want great deals on Grace in LA jeans, it's time to shop Cavender's. We offer a beautiful selection of Grace jeans in the styles you want. Shop for Grace LA ...
Grace in LA Jeans - Sheplers
Free Shipping on orders of $99 or more at Sheplers.com! Grace in LA Jeans for Women & Girls bring style and comfortable fit to dazzling Denim Jeans & Shorts.
Grace In LA Women's Jeans | eBay
Shop from the world's largest selection and best deals for Grace In LA Women's Jeans. Shop with confidence on eBay!
Grace in LA Jeans for Women, Kids & Plus Size
Grace in LA Jeans are available here in not only junior's sizes, but plus size & girl's sizes as well. Junior's Grace in LA denim is available in waist sizes 24-31.
Amazon.com: Grace in LA - Jeans / Clothing: Clothing ...
Women's Jeans from Amazon.com . Finding that perfect pair of women's jeans can make all the difference. Whether your wear-anywhere favorites are dark-wash skinny ...
Grace in LA – Grace in LA - Denim
Women's Denim by Grace in LA. Embellished denim jeans.
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@TheAlexP: Karate classes... Because breaking boards on your head is all cool and shit if a House ever starts attacking you.
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@Cherbearxo: Apparently it's okay for the office to have "casual Friday's," but "nudist Tuesday's" are frowned upon. How embarrassing for me.
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