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Endogenous cardiotonic steroids, through their interaction with the ouabain-binding site of the Na-K-ATPase α-subunit, have been implicated in a variety of cardiovascular disease states including hypertension. We have previously shown that ACTH-induced hypertension is abolished in mutant mice expressing ouabain-resistant α1- and α2-subunits. To further evaluate hypertension resistance in these mutant mice, we examined blood pressure changes in a modified model of 2-kidney, 1-clip (2K1C) renovascular hypertension. To reliably generate 2K1C hypertension, we used polyvinyl tubing (inner diameter: ∼0.27 mm) to accurately gauge the degree of renal artery stenosis. Using this method, virtually all of the clipped mice became hypertensive and there was no incidence of apparent renal ischemia. By telemetry, in response to renal artery clipping, blood pressure in wild-type mice (α1 ouabain-resistant, α2 ouabain-sensitive) increased from 97 ± 3 to 136 ± 7 mmHg. In α1-resistant, α2-resistant mice, pressure increased from 93 ± 2 to 123 ± 4 mmHg, and in α1-sensitive, α2-resistant mice, blood pressure increased from 95 ± 2 to 139 ± 5 mmHg. Blood pressure changes were equivalent in all three groups. In sham mice, blood pressure did not change (96 ± 1 to 95 ± 2 mmHg). Renin mRNA expression was dramatically elevated in the left vs. the right kidney, and plasma renin concentration was elevated similarly in all genotypes. These data indicate that sensitivity of the α1- or α2-Na-K-ATPase binding site to cardiotonic steroids is not a prerequisite for the development of 2K1C renovascular hypertension. In addition, use of a polyurethane cuff to constrict the renal artery provides a reliable method for producing 2K1C hypertension in mice.
- cardiotonic steroids
- telemetry
- blood pressure
endogenous cardiotonic steroids have been implicated in the pathogenesis of a number of cardiovascular disease states including human heart failure and hypertension (1). Elevated levels of these compounds have been reported in essential hypertension, renovascular hypertension, and preeclampsia (12, 14, 27). Commonly, these compounds are thought to bind to and inhibit the α-subunit of the Na-K-ATPase (α-NKA), of which there are four known isoforms (designated α1–4). The subsequent elevation in intracellular Na+ reduces Na+/Ca2+ exchanger activity, resulting in an increase in free intracellular Ca2+ concentration that is responsible for the positive inotropic effects in cardiac and smooth muscle. More recently, it has been proposed that these compounds can initiate specific intracellular signaling cascades that produce various physiological responses (1). In either case, high-affinity binding of the steroid to the α-NKA subunit is required to elicit a biological effect. Thus recent development of mutant mice with altered sensitivity of the α1- and α2-NKA isoforms to cardiotonic steroids has presented the opportunity to examine the specific role of these subunits in physiology and pathophysiology.
Accordingly, since previous studies had reported elevated levels of circulating ouabain-like compounds during ACTH-induced hypertension (39), we examined ACTH hypertension in our α-NKA mutant mice and found that blood pressure increases were markedly blunted in mutant mice expressing "ouabain-resistant" α1- and α2-NKA subunits, compared with mice in which either subunit is "ouabain sensitive" (10, 24). These findings confirmed that this form of stress-related hypertension is dependent upon the interaction between endogenous cardiotonic steroids and the α1- and/or α2- subunit. By contrast, it has been argued that the Na-K-ATPase plays a role in salt-dependent, but not salt-independent hypertension (16). We recently reported, however, that DOCA-salt hypertension persists in these α1/α2 ouabain-resistant mice (23). We therefore sought, in the present study, to examine the effects of altered ouabain sensitivity in a two-kidney, 1-clip (2K1C) model of high-renin, salt-independent, renovascular hypertension. Based on previous observations, we hypothesized that blood pressure increases would not differ between ouabain-sensitive and ouabain-resistant mice.
To perform these experiments successfully, it was necessary to develop a reliable and consistent model of 2K1C hypertension in the mouse, and we postulated that the use of a polyurethane cuff instead of a metal clip placed on the renal artery would provide this needed consistency. We provide data to validate this modified surgical approach that reliably and rapidly increased blood pressure in mice.
Animal model.
Experiments were carried out with mutant mice expressing NKA isoforms with altered ouabain sensitivities: α1-resistant/α2-sensitive (α1R/R2S/S, wild-type), α1-resistant/α2-resistant (α1R/Rα2R/R, single-mutant), and α1-sensitive/α2-resistant (α1S/Sα2R/R, double-mutant). Alterations in ouabain sensitivity were produced by targeted mutations that produced amino acid substitutions at position 111 and 122 in the first extracellular loop of the α-subunit, as previously described (8, 9). Animals were obtained from two established colonies at the University of Cincinnati, which were both maintained on a mixed 129SvJ and Black Swiss background. The colony of single-mutant α1R/Rα2R/R mice were maintained by mating heterozygous male and female animals (α1R/Rα2S/R × α1R/Rα2S/R) (8). The colony of double-mutant (α1S/Sα2R/R) mice were maintained by mating homozygous double-mutant animals. Breeding pairs were periodically backcrossed to a parallel subcolony of wild-type mice to sustain a consistent genetic background between mutant and wild-type mice. Wild-type animals were obtained from both colonies and show no differences in any observed measurements. Genotypes were determined by PCR analysis of DNA from tail biopsies, as described. All experiments were approved by the University of Cincinnati Institutional Animal Care and Use Committee in accordance with established guidelines.
2K1C model.
The Goldblatt model of 2K1C renovascular hypertension has been successfully reported in mice using the standard approach of clipping the renal artery with a silver clip, usually a 0.13-mm slit (6, 17, 38). However, we and others have found this approach to be unpredictable and inconsistent, as even small variations in slit dimensions result in insufficient constriction or renal ischemia. As an alternative, we used a small segment of polyurethane tubing, sliced lengthwise, to act as a cuff to produce a constriction around the left renal artery [MRE 025, Braintree Scientific; internal diameter (ID) = 0.30 mm; outside diameter (OD) = 0.63 mm; wall thickness, (WT) = 0.16 mm]. Since placement of a plastic cuff would result in constriction in two dimensions (constriction) rather than one (flattening), as with a metal clip, it was apparent that the appropriate cuff diameter would be substantially larger than 0.13. For reference, the OD of the renal artery in our mice typically ranges from 0.3 to 0.4 mm. Our initial attempts to test cuff size indicated that cuffs with an ID <0.2 mm resulted in blanching of the kidney and apparent cessation of renal blood flow. We therefore tested cuff diameters of 0.20, 0.24, and 0.27 mm in three separate pilot groups of NIH Swiss wild-type mice. As illustrated in Fig. 1, small segments of the circumference of the tubing could be removed so that cuffs with these calibers could easily be produced. The diameters noted above were achieved by removing ∼0.31, 0.19, and 0.10 mm of the circumference, respectively. In practice, the amount removed was estimated by recognizing that the wall thickness of the tubing was 0.16 mm, and then removing a segment that was relatively larger, smaller, or equivalent to that wall thickness; after cutting, the resulting cuff diameter was measured. For cuff placement, mice were anesthetized with isoflurane, placed on their right side, and the left renal artery was exposed and dissected free through a flank incision. The cuff was then placed around the renal artery and secured by gently tying with a 7-0 silk ligature to close the gap in the cuff. Sham mice underwent the same procedure, but the cuff was not placed.
After 1 wk, animals were anesthetized with isoflurane for direct recording of blood pressure and analysis of right and left kidney renin mRNA (see below). In a subset of mice, systolic blood pressure was measured by tail-cuff before and after clipping (Visitech, BP2000, Apex, NC). We observed that blood pressure was significantly elevated in mice with 0.27-mm cuffs compared with sham, but unchanged in those with 0.20- or 0.24-mm cuffs (Fig. 2A). The left-to-right renin mRNA ratio was not different from 1 in the sham group and in the 0.24-mm group (0.88 ± 0.1 and 0.78 ± 0.03, respectively). In the 0.27-mm group, renin mRNA expression was significantly elevated in the left kidney, with a left-to right ratio of 3.0 ± 0.9. In the 0.20-mm group, left kidney renin expression was markedly suppressed, with a ratio of 0.18 ± 0.02. It was noted that the left kidneys from these mice appeared profoundly ischemic upon excision. In a subset of mice, we measured tail-cuff blood pressure in sham controls and in mice with 0.27-mm cuffs, and the results are shown in Fig. 2B. We found that within 48 h after cuff placement, systolic blood pressure increased significantly in the 0.27-mm 2K1C mice. We therefore chose a cuff size of ∼0.27 mm to perform all of the remaining studies.
Telemetric measurement of blood pressure.
In α1R/R2S/S, α1R/Rα2R/R, and α1S/Sα2R/R mice, continuous blood pressure recordings were made as previously described using the TA11PA-C10 pressure transmitter (Data Sciences International, St. Paul, MN)(24R/Rα2S/S and α1R/Rα2R/R mice were conducted first, and these mice were euthanized at 6–7 days to correlate renal renin expression with changes in blood pressure. Experiments in α1S/Sα2R/R mice were performed at a later time, and these mice were euthanized at 4 wk to examine the long-term time course of blood pressure changes in response to renal artery stenosis. Three mice of each genotype underwent sham surgery, and data were pooled.
Evaluation of kidney renin mRNA and plasma renin concentration.
Kidney renin mRNA was measured by real-time PCR. At the time of death, total RNA was isolated from left and right kidneys using Tri-reagent (TR118; Molecular Research Center). RNA concentration was quantified by UV spectrophotometry, and samples were stored at −80°C. cDNA was synthesized using oligo dT primers in the SuperScript III first-strand synthesis system (18080–051; Invitrogen). Real-time PCR analysis was carried out using the iQ SYBR Green system (170–8882; Bio-Rad Laboratories) and the Opticon2 DNA Engine (MJ Research). Levels of GAPDH and renin were determined using primers from PrimerBank (GAPDH: PrimerBank ID 6679937a3; renin: Primer Bank ID 13676837a1) (31, 34) and Opticon Monitor Analysis Software (Ver. 2.02). All reactions were run in triplicate, and renin levels were normalized to GAPDH levels.
For measurements of plasma renin concentration (PRC), blood samples were obtained from conscious mice at 1 wk of 2K1C by tail-cut using EDTA as an anticoagulant and assayed as previously described (26). From each sample, 2 μl of plasma was added to an incubation cocktail containing 158 μl of modified phosphate incubation buffer (0.1 M phosphate buffer, 0.06 M disodium EDTA, 0.2% gelatin, pH 6.5), 2 μl of PMSF, and 138 μl substrate (containing 250 ng angiotensinogen, obtained from 48-h nephrectomized rats). This sample was incubated at 37°C for 90 min, boiled for 10 min to stop the reaction, centrifuged for 10 min at 2,500 g, and the supernatant was removed and frozen for later analysis (typically in <48 h). ANG I generation was analyzed by radioimmunoassay using a Gamma Coat PRA kit (DiaSorin, Stillwater, MN) according to the manufacturer's instructions. The assay was run with buffer blanks and internal quality controls.
Statistical analysis.
Data were analyzed using a one-way or mixed two-way ANOVA, with repeated measures on the second factor. Post hoc analysis for pairwise comparisons was performed using the Holmes-Sidak test (SigmaPlot). Summary data are presented as the means ± SE.
A total of 32 mice were prepared for telemetry. Of these, one mouse died perioperatively and one mouse did not sustain a patent catheter beyond 5 days. The remainder were included in the analysis. As shown in Fig. 3, blood pressure increased significantly in all three genotype groups of mice within 1 day of cuff placement and continued to rise gradually over the next 3 days. Blood pressure did not change in sham mice. Compared with α1R/Rα2S/S and α1S/Sα2R/R, blood pressure in the α1R/Rα2R/R mice was ∼10 mmHg lower in the days following cuff placement, but this difference was not significant. To examine the reliability and consistency of hypertension development in the 2K1C mice, blood pressure plateau values on days 4–6 were averaged and compared with the baseline average from day −3 to −1 in each mouse. Nine mice received sham surgery, and 21 mice were implanted with renal artery cuffs. Data from each mouse are shown in Fig. 4, along with group averages. Blood pressure increased by at least 15 mmHg in 18 of 20 mice studied. In one α1S/Sα2R/R mouse, the increase was 12.5 mmHg. In one α1R/Rα2R/R mouse, there was only a 5-mmHg increase in blood pressure, but it was discovered at necropsy that the cuff had slipped off the artery at some point after implantation. Data from this mouse, denoted by ×-symbols in Fig. 4 (second panel from the left), were omitted from the statistical analysis. None of the 20 clipped kidneys exhibited overt signs of ischemic damage at necropsy. Statistical analysis of plateau levels (days 4–6) indicated that the increase in blood pressure was not significantly different between the three genotypes.
At termination of the experiments, both left and right kidneys were carefully dissected and weighed, and the cuff was removed intact for determination of in situ diameter. Cuffs were photographed with ligatures in place, and the lumen diameter was measured using NIH ImageJ. Left and right kidney weights and cuff diameters are given in Table 1. The cuff diameter for all 2K1C mice was 0.276 ± 0.003 mm and was not different between the three groups of clipped mice. Other morphometrical data are presented in Table 2. After 1 wk of 2K1C hypertension, both α1R/Rα2R/R and α1R/Rα2S/S mice showed modest, but significant decreases in left kidney weight and left/right kidney weight ratio. There was no evidence of cardiac hypertrophy after 1 wk. As shown in Fig. 5, at 1 wk of left renal artery clip, the relative expression of renal renin mRNA was markedly upregulated in the left kidney of both α1R/Rα2R/R and α1R/Rα2S/S mice, by 19- and 22-fold, respectively.
In the 2K1C α1S/Sα2R/R mice, we opted to extend the 2K1C hypertension period to 4 wk to determine whether blood pressure elevation was sustained for an extended period. Importantly, we observed in these mice that blood pressure was highest at 7 days and thereafter tended to decline to various degrees in each mouse, such that by day 21 the difference from baseline was not significant, as shown in Fig. 6. This decline in pressure was associated with a substantial reduction in left kidney weight and increase in right kidney weight by 4 wk, as shown in Table 2. There was also some indication of cardiac hypertrophy in these mice. Also, at 4 wk in α1S/Sα2R/R mice, the relative expression of renin mRNA in both the right and left kidneys was substantially reduced (Fig. 5), but the expression of renin mRNA in the left kidney remained markedly higher than in the right.
PRC was measured in all groups of mice at 1 wk of 2K1C hypertension. Compared with the sham value of 0.30 ± 0.05 μg ANG I·ml−1·h−1 (n = 7), 2K1C mice had significantly elevated levels of PRC (P = 0.022): 1.79 ± 0.40 μg ANG I·ml−1·h−1 in α1R/Rα2R/R, (n = 5) 2.27 ± 1.02 μg ANG I·ml−1·h−1 in α1R/Rα2S/S, (n = 6), and 3.19 ± 0.80 μg ANG I·ml−1·h−1 in α1S/Sα2R/R mice (n = 6). PRC values between the three groups of 2K1C mice were not significantly different.
Role of NKA α-subunit and its endogenous ligands in renovascular hypertension.
We have previously reported that ACTH-induced hypertension is blunted in mutant mice expressing a ouabain-resistant α2-Na-K-ATPase subunit (10, 24). Our explanation of this finding was that this form of hypertension was dependent on elevated levels of an endogenous cardiotonic steroid ligand that interacts with the α2-subunit to produce this form of hypertension. It has been argued that these endogenous ouabain-like compounds contribute to other forms of hypertension as well, including essential hypertension (2, 15), preeclampsia (12, 33), and DOCA-salt hypertension (14, 19, 32). Although ACTH-induced hypertension is commonly regarded as a stress-related form of hypertension involving multiple regulatory pathways, the pathogenesis of this form of high blood pressure remains somewhat enigmatic, and there appears to be a substantial amount of species heterogeneity (37). It therefore became important to determine whether resistance to hypertension in the α1R/Rα2R/R animals was a general phenomenon, or alternatively, whether the resistance was peculiar to this form of stress-related hypertension. Accordingly, we recently reported that DOCA-salt hypertension persists in mice with a ouabain-resistant α2-subunit, which argues against a particular role for this isoform in salt-dependent hypertension (23).
The purpose of this study, therefore, was to explore the role of the α1- and α2-isoforms in a third well-characterized model of experimental hypertension, specifically the 2K1C model of renovascular hypertension. We demonstrated that in response to unilateral renal artery constriction, increases in blood pressure were statistically equivalent in ouabain-resistant mice (α1R/Rα2R/R) compared with mice with ouabain-sensitive α1- and α2-subunits (α1R/Rα2S/S and α1S/Sα2R/R). Based on the data shown in Figs. 3 and 4, it is tempting to consider the possibility that the blood pressure increase seen in the α1R/Rα2R/R mice might be blunted to some degree since the change in blood pressure at its plateau was ∼30 mmHg in the α1R/Rα2R/R mice vs. 39 and 44 mmHg in the α1R/Rα2S/S and α1S/Sα2R/R mice, respectively. We cannot rule out the possibility that a more extensive study with more mice might better define a subtle influence of ouabain sensitivity. Nevertheless, our data definitively demonstrate that the primary mechanisms that elevate blood pressure in 2K1C hypertension do not require α1 or α2 ouabain sensitivity. We can allow for the possibility, however, that endogenous cardiotonic steroids interacting with the α1- and/or α2-subunits might modestly augment the hypertensive response.
Four α-isoforms of Na-K-ATPase, α1–α4, have been identified, and expression levels are tissue and species dependent (22). The α1-isoform is expressed abundantly in most tissues; the α2-isoform is detected in brain, heart, skeletal and vascular smooth muscle, and adipocytes; the α3-isoform is predominant in neurons and ovaries; the α4-isoform is exclusively expressed in sperm. In mice, it has been reported that in both cardiac muscle and vascular smooth muscle, the expression of the α1-isoform represents ∼70% of the total, and the α2 represents ∼30% of the total (29). Renal epithelial cells express almost exclusively the α1-isoform (11, 25). Since other α-isoforms (α3 or α4) have not been detected in these cardiovascular/renal cell types, our present data suggest that endogenous cardiotonic steroids acting on the heart, vasculature, or kidney do not play a substantial role in the pathogenesis of renovascular hypertension. On the other hand, since both α1R/Rα2S/S and α1R/Rα2R/R mice express a ouabain-sensitive α3-subunit, which is a dominant isoform in neurons, a potential role for the α3-Na-K-ATPase in 2K1C renovascular hypertension remains possible. Such a role for endogenous cardiotonic steroids acting in the central and peripheral nervous system to promote hypertension has been extensively investigated and is well supported (21).
Modified approach for 2K1C hypertension in mice.
In developing a useful model of 2K1C Goldblatt hypertension for the mouse, our original attempts using conventional silver clips, with slit dimensions ranging from 0.11 to 0.13 mm, resulted in low success rates. We found that in more than half of the mice, the degree of constriction was either too small, leading to insufficient blood flow restriction, or too great, leading to global renal ischemia. In either case, no hypertension developed. Since the result of this inconsistency would inevitably lead to artifactual selection of our treatment groups, and since this self-selection process could very likely be inconsistent between our genotypes, we found it necessary to seek a more reliable and consistent approach for generating 2K1C renovascular hypertension in our mice. We reasoned that application of a circumferential constriction, in the form of a polyurethane cuff would provide better control of the caliber of the constriction and blood flow restriction. We originally experimented with a variety of tubing types (polyethylene, Teflon, and polyurethane) with various diameters and wall thickness but determined that the MRE 025 polyurethane tubing (Braintree) offered the best advantages: it could be easily trimmed to the desired caliber, was highly biocompatible, and could be securely tied in place without compressing. Based on our preliminary experiments, we determined that a target cuff diameter of ∼0.27 mm produced the most consistent results. Actual cuff diameter ranged from 0.25 to 0.29 mm, based on postmortem analyses. Our results demonstrate the effectiveness of this approach. Of the 30 mice used in our telemetry experiments, 9 were designated for sham surgery, and 21 received renal artery cuffs. Of these 21 mice, all but one developed some meaningful degree of hypertension (i.e., >10 mmHg). In the mouse with no hypertension, it was discovered at necropsy that the polyethylene cuff had become free from the renal artery postoperatively and there was no constriction. Upon gross examination at the end of the experiment, there was no apparent indication of overt renal ischemia in any of the clipped kidneys.
Other investigators have used the silver clip (or stainless steel) approach in mice with various levels of success. One of the earliest and most thorough investigations in mice was reported by Wiesel et al. (38) in 1997. They evaluated stainless steel clips with openings ranging from 0.04 to 0.18 mm and found that the optimal opening size was 0.12 mm. They reported that openings of below 0.11 mm always resulted in infarction, and those larger than 0.12 mm did not produce hypertension. Despite the constraints of these tight tolerances (<0.02 mm), the incidence of infarction at 2–4 wk remained significant in both the 0.11-mm group (40–80%) and the 0.12-mm group (15%). By contrast, despite the fact that the diameter of our cuffs varied from 0.25 to 0.29 mm, our success rate for producing hypertension was nearly 100%, and the incidence of infarction was nil.
While Wiesel et al. (38) did not examine the time course of hypertension, other groups using the metal clip approach have reported that full development of hypertension requires often 7–14 days (4, 5, 20) whereas others have found that it occurs within 1–3 days (13, 28). In rats and dogs, the development of 2K1C hypertension has been found to be quite variable, and to some extent, investigator and technique dependent (3, 18, 36). Highlighting this variability, Sigmon et al. (30) showed in rats that placement of metal clips resulting in a wide range of flow restriction could produce increases in pressure that were quantitatively similar, but qualitatively different in terms of mechanism. Using our approach for 2K1C hypertension, we found that blood pressure increased markedly in the first 24 h following placement of the renal artery cuff and that blood pressure plateaued at its maximum value within 3–4 days (Fig. 3). This response was consistent in all of the mice analyzed. This model also demonstrated many of the other hallmarks of renovascular hypertension, including elevation of renin mRNA expression in the clipped kidney, sustained elevation of plasma renin concentration, and an increase in the ratio of left to right kidney weight.
Experiments in the α1S/Sα2R/R mice were conducted after the other groups were completed, and in these mice we elected to follow the progression of hypertension over a 4-wk period, rather than euthanizing them at 1 wk. We discovered that the blood pressure peaked at 7 days and thereafter tended to decline. In two of the mice, blood pressure returned nearly to baseline (see Fig. 6), and we found that the kidneys of these mice had atrophied markedly. It appears that with increasing time, there was a progressive stenosis in these two mice that eventually led to complete renal ischemia, and consequent resolution of the hypertension. Similar declines have been observed with conventional renal artery clips in both rats and mice (13, 18), but sustained increases have also been reported for both species (4, 7, 17). It is possible that the progressive stenosis observed in the α1S/Sα2R/R mice is not a general phenomenon but may be peculiar to these mutant mice specifically. Indeed, we have recently reported that the α1S/Sα2R/R mice are more susceptible to pressure overload-induced myocardial fibrosis (35). It is not unreasonable to suppose that a similar phenomenon may occur in the renal vasculature in response to unilateral stenosis. In any case, the chronic phase of 2K1C hypertension in mice, using this method or the conventional metal clip approach, requires further investigation.
In summary, we have developed and validated a modified approach for 2K1C renovascular hypertension that reliably and consistently elevates blood pressure by 20–40 mmHg. The facile nature of this method should simplify the use of this model in the mouse and could be extended for use in rats. Using this model, our data indicate that the development of 2K1C hypertension in mice does not depend on the ouabain sensitivity of either the α1- or α2-Na-K-ATPase. These data imply that circulating cardiotonic steroids acting on the heart or vasculature do not participate in the pathogenesis of this form of hypertension.
This work was supported by National Institutes of Health (NIH) Grants DK57552 (to J. N. Lorenz), HL 28573 and HL 28573 (to J. B. Lingrel), PPG 5PO1HL090550-02 (to W. H. Beierwaltes), and DK050594 (to G. E. Shull).
No conflicts of interest, financial or otherwise, are declared by the authors.
- Copyright © 2011 the American Physiological Society


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ECU's Jeff Hoffman out for the year
: : Story
Frankie Piliere
Wednesday, May 07, 2014
MLB Draft Coverage schedule
College Top 50 prospects
East Carolina righthanded pitcher Jeff Hoffman, the No. 2 college prospect for the upcoming Major League Baseball draft, was working toward solidifying his case as a number one overall pick candidate this spring, and recently had been opening up a lot of eyes. Now, his 2014 season is over. As of Tuesday, Hoffman has been shut down for the remainder of the college baseball season, and has opted to have Tommy John surgery after consulting with Dr. James Andrews on Monday.
After being shut down a couple weeks ago for what was believed to be minor elbow swelling, it was discovered this week the ECU righty has a small tear in his right elbow and will indeed require surgery.
The surgery is scheduled for next week with Dr. Andrews. Early indications show that Hoffman is still potentially being considered by teams (who already have knowledge of the situation) in the first 5-10 picks. But, it's also too early to speculate just how this may impact his stock.
The news is a major setback for the righty. We saw him at a recent performance at Rice University, where he touched 98 in the eighth inning, and showcased a plus curveball and changeup. Overall, Hoffman's junior campaign ends with a 2.94 ERA in 67 1/3 innings, along with 72 strikeouts and 20 walks.
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A Royal Fill-Them-in Tale
In this writing worksheet, students read a story about a royal family and complete the tale by filling in missing words. Students may choose any word they like but must follow the directions which tell what kind of word (adjective, verb, color word, boy's name, etc.) to fill in.
3rd - 4th English Language Arts 3 Views 2 Downloads
Resource Details
Start Your Free Trial
Save time and discover engaging curriculum for your classroom. Reviewed and rated by trusted, credentialed teachers.Try It Free
Joseph Had a Little Overcoat: Flash Card Games
Expand the vocabulary of young readers with this series of five activities based the children's book Joseph Had a Little Overcoat by Simms Taback. From playing bingo to group storytelling, a variety of different approaches are presented...
1st - 3rd English Language Arts CCSS: Adaptable
Catching the Sun: Tales from Asia
Explore the folklore of Asia and the South Pacific with this language arts lesson series. Complementing a reading of Catching the Sun: Tales from Asia by Jan M. Mike, this resource supports learners with understanding cause and effect...
3rd - 6th English Language Arts CCSS: Adaptable
Compound Word Trivia
Engage young learners in expanding their vocabulary with these fun games and activities. Children learn how compound words, root words, and affixes provide clues about the meaning of unfamiliar vocabulary. These six activities get...
1st - 4th English Language Arts CCSS: Adaptable
Folk and Fairy Tale Readers: The Nightingale
Engage emergent readers in a literature unit on fairy tales with this printable version of "The Nightingale." Including dialogue, multisyllabic words, and up to five lines of text on a page, this book is ideal for developing the reading...
Pre-K - 3rd English Language Arts CCSS: Adaptable
Folk and Fairy Tale Readers: The Elves and the Shoemaker
Engage young readers in a unit on fairy tales with their very own copy of the classic story "The Elves and the Shoemaker." Including dialogue, three-syllable words, and up to five lines of text on a page, this printable book is best...
Pre-K - 3rd English Language Arts CCSS: Adaptable
Greek and Latin Roots, Prefixes, and Suffixes
How can adding a prefix or suffix to a root word create an entirely new word? Study a packet of resources that focuses on Greek and Latin roots, as well as different prefixes and suffixes that learners can use for easy reference
3rd - 8th English Language Arts CCSS: Adaptable
Fairy Tales and Tall Tales - Read-Aloud Anthology
Enrich a unit on fairy tales and tall tales with a set of read-aloud lessons. Second graders hone writing, vocabulary, comprehension, and literary analysis skills as they read classic stories. Complete with extension projects, discussion...
2nd - 4th English Language Arts CCSS: Designed
Narrative Writing: Using Exact Words
Review the narrative writing process with your emerging story writers. They read a sample narrative and identify five vague verbs that could be replaced with a more exact, exciting verb. Then they write a personal narrative making sure...
3rd - 6th English Language Arts CCSS: Designed
Mini-Lesson Planning for Inferences
Making inferences and drawing conclusions is a key component to successful active reading. Encourage your class to use context clues and prior knowledge to infer different elements of a story, including the setting, plot, and character...
4th - 7th English Language Arts CCSS: Adaptable
What Members Say
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- Belvidere, NJ


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Republicans will vote Tuesday in the Idaho, Michigan and Mississippi primaries and the Hawaii Republican caucuses. Democrats in Michigan and Mississippi are also holding their primaries. In total, 205 delegates are at stake for the two Democratic candidates.
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W32/Delf Trojan removal utility Publisher's description
from Proland Software
This virus removal tool will detect and remove W32/Delf Trojan.
cleandelf.exe will detect and remove W32/Delf Trojan and its variants completely, from your system. Download the cleandelf.exe and save it in a temporary directory. Double click on cleandelf.exe to execute it. Follow the user-friendly instructions to detect and remove the W32/Delf Trojan and its variants from your computer.
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Program Release Status: New Release
Program Install Support: No Install Support


text:
Welcome to Librarium Online!
Join our community of 80,000+ members and take part in the number one resource for Warhammer and Warhammer 40K discussion!
Registering gives you full access to take part in discussions, upload pictures, contact other members and search everything!
Hi everybody...
I'm new to this forum, and this is my very first list....
Please, rate it....
The List:
Hero Choice
General Stephan von Kessel - Captain 78 points
General, warhorse, barding, lance, full plate armour, shield
(With the knights)
Captain Albin Muller - Captain 84 points
Grat Weapon, Armour of Meteoric Metal, Biting Blade
(With the Black Lion Regiment)
Core Choice
Knightly Order - 7 - 201 points
Full command, warhorse, barding, lance, full plate armour, shield
Black Lion Regiment - 24 Swordsmen - 199 points
Light armour, shield, Stendard, musician
Detatchment 1 - 5 Handgunners
Black Eagle Regiment - 25 Swordsmen - 215 points
Light armour, shield, full command
Detatchment 1 - 5 Handgunners
Handgunners - 12 - 121 points
Marksman with Hochland Long Rifle
Special Choice
Grat Cannon "Rage of Sigmar" - 100 points
Total: 998 points
Models in Army: 84
Dispel Pool: 2
Cast Pool: 2
So... What do you Think????
Nuln is famous for it's pikemen yet you have not even a single spearmen regiment.
Apart from that and the above rather helpful comments I'ld say to drop the biting blade for a halberd (if you still can).
Up, up and away!


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This spacious family owned and managed home features hand carved furniture & fantastic 180 degree ocean views to Molokai. The remodel, completed in 2012, includes granite counter tops, tile floors, stainless steel appliances, & air conditioning. The two separated bedrooms each have a king-sized bed & adjoining expansive bathrooms. The master bedroom boasts a small wall safe for your valuables & a separate lanai overlooking the ocean. The views from every window are private & landscaped. The couch in the living room makes into a queen-sized bed. The 3rd, or guest, bathroom is complete with a tile shower & is...
accessible to your guests or for those sleeping in the living room. A Graco Pack & Play is available for the little ones. Three HD TV's, Wi-fi-, iPod dock, 5 telephones with free long distance (to the U.S. & Canada), cable TV, & a high speed Roadrunner Turbo internet connection keep you in touch with the world while the plush lanai furniture beckons the visitor to sit back to enjoy the ocean views & a cold beverage .Fine beaches, judged by many as the best in Maui, are minutes away either by walking; your car; or the free, door-to-door Kapalua Resort Shuttle. Dennis and I are 70 years old. We can walk to Oneloa Bay, below the villa, in less than 5 minutes. Coming back up the hill takes a little longer!Located at the end of a short cul-de-sac this front row villa has panoramic views of the ocean & the island of Molokai. In season whale watching from the lanai is a MUST (with provided telescope & binoculars). Although located just minutes from busy Lahaina, the villa is situated in a lavishly landscaped & peaceful setting. Our end of the island benefits from the Trade Winds which you will find are very welcome! It's a short walk to the nearest Association pool with well maintained BBQ's & a covered outdoor kitchen/dining area (incl. refrigerator, stove, microwave, sink, library & seating area). We would love to welcome you to Maui and our home.Beds: 2 King Beds, 1 Queen Bed, 1 Sofa Bed.
|Rental Fee
|$301 - $350
|Property Type
|Kapalua Vacation Home-Panoramic Ocean Views
|Not available
|Pets Allowed
|Please inquire
|Provided By
|Direct Link
|Last Updated
|4 days ago
Minimum stay required: 6 nights. Maximum occupancy: 6 guests. Lodging tax: 13.42%Tax I.D.: W08706009-01Deposits: 1/3 due immediately.1/3 due 60 days before arrival.1/3 due 30 days before arrival.100% refund up to 60 days before arrival.Payment Options: Cashier's Check, Mastercard, PayPal, Personal Check, VisaOther Fees: $210.00 Cleaning fee.
Vacation rental in the Kapalua Vacation Home-Panoramic Ocean Views community in Lahaina, HI 96767 has 2 beds, 3 baths and available to rent for $301 - $350. The property was listed on RentalSource 25 days ago via FlipKey. The property is in zip code 96767 which is located in Lahaina, HI.Report listing | Visit website
|Jul 31, 2016 - Dec 23, 2016
|$301 /day
|Jan 1, 2017 - Apr 1, 2017
|$350 /day
|Apr 2, 2017 - Dec 15, 2017
|$325 /day
Beware of rental scams! Situations involving wire transfers or a landlord who can't meet you in person can be red flags.
All information listed is provided by third parties and is deemed reliable, but should be independently verified. Price and specifications are subject to change without notice. RentalSource provides this service without representations or warranties of any kind either expressed or implied.


text:
'Professors Crew and Kleindorfer have once again assembled a valuable collection of essays that address timely and important issues in postal sectors throughout the world. The essays employ diverse methodologies to provide useful insights about recent and likely future developments in the postal industry. This book will be a valuable resource for researchers, industry practitioners, and policymakers alike.' a David E.M. Sappington, University of Florida, US In our increasingly technology-focused world, demand for traditional postal services is steadily shrinking. This timely volume examines the many challenges that the worldwide postal sector is facing as a result of growing electronic competition, and offers expert recommendations for reshaping postal structures to strengthen their competitiveness in an electronic age. Drawn from a selection of papers presented at the 20th Conference on Postal and Delivery Economics in Brighton, UK, this book showcases expert contributions on the rapidly changing postal sectors in both the United States and Europe. Topics discussed include the various financial challenges posed by decreasing demand for postal services, recent changes in how postal services are provided, and new structures and modes of operation, such as privatization, that are currently affecting the industry. Contributors offer a thorough breakdown of the issues as well as ideas for keeping the postal sector alive in a world that is growing ever more reliant on purely electronic means of communication. Economists with an interest in regulatory economics, innovation and public sector economics will find this volume useful and informative, as will institutional libraries and industry professionals.The Act removes certain restrictions on ownership of shares in Royal Mail while providing that Post Office Limited will ... new public scheme for repayment of pension benefits to qualifying members of the current Royal Mail pension plan.63 Theanbsp;...
|Reforming the Postal Sector in the Face of Electronic Competition
|Edward Elgar Publishing - 2013-01-01


text:
Ericdress Mesh Patchwork See-Through Sheath Dress
Ericdress Round Collar Plain Bodycon Sweater Dress
Here ericdress.com shows customers a fashion collection of current pink and black cocktail dress.You can find many great items. They all have high quality and reasonable price. You can get big discount sometimes, because we always do promotions. If you need pink and black cocktail dress,come here to choose. We give you good items and services.


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Harnessing the Networking Potential of Career Center Events
Every day on the Endicott College campus and across the community, students are afforded opportunities to network with potential employers…. read more
Every day on the Endicott College campus and across the community, students are afforded opportunities to network with potential employers…. read more
As we reflect back on Tuesday, September 13th, a number of words come to mind – community being at the… read more
The tragedy of the September 11th terrorist attacks forever shook our nation to its core. Each year, thousands gather to… read more
Leaving home, your personal comfort zone and safety net after 17+ years, can be a difficult transition. Endicott College aims… read more
Internships and job preparation through experience are a huge part of education at Endicott, so we do our best to… read more
Endicott students take community service very seriously – we continually are very proud to announce that total donated hours are well above national averages. Take a look at the amazing things Endicott Faculty, Students and Staff got involved with over the last year!
Big congratulations to senior Kelsey Clark, an Interior Design major, who not only has a fantastic position lined up in… read more
Earlier this month, Endicott was part of The International Robotic Sailing Competition, or SailBot 2013, an innovative event held annually… read more
Congratulations to Endicott's DECA team, who participated in the International Career Development Conference last month in Anaheim, CA and came… read more
Student Emily Pineau is the first to appear in Ibbetson Street Press' Young Poet Series.


text:
'American Idol': No Doubt's Gwen Stefani, Tony Kanal to Mentor Top 8 (Exclusive)
Two of the Grammy-winning band's four members will help the finalists on '80s week -- an appropriate fit considering No Doubt had a hit in 2003 with a song from 1985, Talk Talk's "It's My Life."
Half of No Doubt will appear on American Idol's next week as singer Gwen Stefani and bassist Tony Kanal will mentor the remaining finalists, The theme: '80s night, which is a perfect fit for the Orange County-bred band, who were not only teenagers during the decade but had a Top 10 single in 2003 with a cover of Talk Talk's 1985 hit, "It's My Life."
VIDEO: Nicki Minaj Says It Takes 'Guts,' 'Heart' to Compete on 'American Idol'
No Doubt is signed to Interscope, the label headed by Idol perma-mentor Jimmy Iovine. Stefani has appeared on Idol before, perhaps most memorably styling the season 10 contestants last year in her own L.A.M.B. line.
Q&A: Jimmy Iovine on Sound, Vision and Why He Signed Madonna and Van HalenThe band members spent five hours with the season 11 finalists Friday. As always, sessions took place at Interscope Records' Santa Monica recording studio complex.
Tune in to Fox on Wednesday to see how the Top 8 fare.
Twitter: @Idol_Worship
What's Hot In Music
Follow Idol Worship
- Gucci Mane and Malcolm Gladwell's Twitter Compliments Reach Tipping Point, Become Grand Love Affair
- Velvet Underground Singer Nico's Life Is Getting the Extremely European Biopic It Deserves
- Tom Hanks Tells the Kind of Halloween Dad Jokes He Was Born to Deliver in a Cut SNL Sketch
- What Happened When Bill Murray Was Honored at the Kennedy Center


text: Went last thurs evening for dinner, very poor menu, waitress staff really not interested in taking order, didn't get what I asked for and also nobody came to ask us if everything was OK all the way through the meal. Food portions very small and not much choice on menu. Overall very poor visit, we always loved the 12th lock... More


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Most users ever online was 10,564, 02-01-2013 at 11:20 AM.
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Nothing..I am just bored and needed to make you look cause i can and i needed to let you know also that i am still lingering around this site, wishing i was still back west riding with all of you instead of breaking my ass with crappy weather( And i thought it was supposed to be nice here).
For all concerned we will be taking in the suzuki line, so now i can't say anything bad about them
Seems like its been a very busy and eventfull two weeks back west for many of you, and believe me, i am still feeling everything that is felt by many of you.
I will be visiting this summer, just not sure as of a date, i'll keep posted. For now, be safe, be merry


text:
Marlborough Properties for Sale
642 Listings Showing 1 to 20 Next »
Deadline Treaty
updated A Wonderful Sunny Home with Stunning Sea Views
The vendors have shown exceptional foresight in developing this residence into a spacious and sunny family home enjoying some of ...
updated Inspired By a Passion For Perfection
Harbour View Heights is in close proximity to Picton Township and the beautiful Picton Waterfront and Queen Charlotte Drive much ...
updated Location and Easy Care
Located on quiet rear site with views to Waikawa Bay Marina. This two story home boasts three double bedrooms, two ...
updated A Home To Fall In Love With
Welcome to this lovely villa, ideal for investors or the larger family and particularly appealing to those who adore and ...
Opportunity Knocks...Deadline Sale
20 ha (approx 50 acres) at the head of the Waikakaho Valley. A gracious four bedroom Queenslander home in need ...
Vendors Have Purchased
Our vendors have moved out, inviting your offer on their cherished, near new, family home in a stand-out Rapaura location ...
Make Special Memories Here
Surrounded by well established native bush this large 6661m2 waterfront lifestyle section offers all day sun, peace and serenity. Create ...
updated Owners Have Purchased - Invest In The Inner Circle
Auction (unless sold prior) 12pm, Fri 11 November 2016 Bayleys, 33 Seymour Street, Blenheim This 1920s inner-circle bungalow offers the ...
Fantastic Buying
Benefits: Extended living, woodburner, double garage workshop, 811m2 fenced section. What a great tidy first home or rental. This home ...
Sell! Says The Motivated Vendors
Auction (will not be sold prior) 12pm, Fri 11 Nov 2016 Bayleys, 33 Seymour Street, Blenheim Our out of town ...
Relocation Dictates Sale
Auction (will not be sold prior) 12pm Fri 11 November 2016 Bayleys, 33 Seymour Street, Blenheim Vendors have accepted a ...
Absent Vendor Instructs Sale!
Deadline Sale (will not be sold prior) 4pm, Wed 9 November 2016 Bayleys, 33 Seymour Street, Blenheim Our Christchurch vendor ...
Elevated Dream with Lush Lifestyle
This breath taking property is beautifully elevated with commanding 360 degree views. Stepping into this Italian inspired home is like ...
What a transformation!! My owners have turned what was already a lovely home, into something very special. From the expansive, ...
Deliciously Different
My owners fell in love with their home the minute they walked through the door. They had to have it ...
Awesome Development Opportunity
Located on Boyce Street in Renwick is this substantial 4 bedroom home on a whopping 8934sqm block, most of which ...
Family Versatility
The owners have already purchased a new build, so take advantage of this large warm 4 bedroom family home which ...
updated Nest, Invest Or Renovate 2125 sqm
When location counts...this one is a winner. One big parcel of land 2125 sqm stretching from Beach Road right through ...
Could This be Your new Home?
You will be impressed with this well designed home, offering 3 double bedrooms and an office, with the master having ...
Retired Vendors Want Your Offer
Offered to the market for the first time, you're guaranteed to want to hold on to this attractive property for ...
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text:
Today's Featured Artist: Michael Godard
Protect your iPhone 7 with a brand new iPhone 7 phone case!
Pixels Exclusive: Introducing the hottest summer accessory of 2016... round beach towels! FREE WORLDWIDE SHIPPING.
Fine Art America has expanded to 14 fulfillment centers in 5 countries!
David Bowie Remembered - A Collection of Unpublished Portraits by Terry O'Neill
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Bicycle Under A Window is a photograph by Pedro L Gili which was uploaded on October 17th, 2012.
More from Pedro L Gili
Comments (31)
Congratulations! on being selected for our Autumn Homepage Feature FAA HOME GALLERY FOR ALL WHO CREATE!!
Excellent! v
Pedro, I already commented, voted and promoted your submission for CFC Round 41 so this is my substitute. Fantastic imagery and I love the black and white with the old bike laying against the brick building in the snow. It gives you that vintage and old feel to the photo. Great details in the brick and in the snow on the bike. Clarity is excellent as you can see very clearly the window and part of the sign on the wall. Excellent image, voted, google, tweet and FB Promoted
Love the vintage quality! v/f
Great image!V
Wow in the SNOW TOO! Awesome photo f/v
very nice! v
Love it! v
very cool image. v
very nice! V
Great composition! Love the detail - especially in the brick wall and the reflection in the window. v /g+1 Pay it forward for today CFC
wonderful image, great take in b/w, feel cold just looking at it, nice one v20 PIF CFC
love it, great in b&w v/g+
Wonderful Pedro :-) V
Very nice job Pedro! V
Great texture!
Great capture Pedro! V
I do like it. William has definitely described what this image brings to my mind. When an image brings emotion, and a story you know a job well done. V/F
Pedro L Gili replied:
Thank you, Sydne! I appreciate your continuous comments on my work.
A strong image, good contrast and the different colored mortar leaving the seat leads me into the rest of the shot. Good job.
Very well dine. V
Brrrr... I feel cold just looking at this. Great capture. v


text:
Fortunately, Christopher knows, and is eager to share his findings with you. To read his full review, simply navigate using the link provided below.
Dragon Quest Builders Oct. 23 • 10am PDT/1pm EDT
Skies of Arcadia Continues! Every Monday • 5pm PDT/8pm EDT
Star Wars: KotOR Continues! Every Tuesday • 10am PDT/1pm EDT
Taking a week off! Every Thursday • 5pm PDT/8pm EDT


text:
|By PR Newswire
Article Rating:
|April 18, 2013 11:39 AM EDT
DALLAS, Pa., April 18, 2013 /PRNewswire/ -- Digital Net Agency, Inc. (DNA), a leading performance-based digital agency, today announced that its affiliate management division is offering Google Affiliate Network Merchants complimentary network migration to the partner network of their choice, effective immediately. In addition, DNA has negotiated with its premier network partners to waive network launch and transaction fees during the migration, as well as to offer flexible contractual terms to match those previously provided by Google Affiliate Network. DNA's program offer comes in the wake of Google's announcement that it will leave the affiliate marketing business and shut its network.
"DNA stands at the forefront of the Google Affiliate Network issue, working with our network partners to help address the needs of affected merchants -- starting with the waiving of all traditional migration fees, no matter how complicated the migration, for any new DNA affiliate management clients," said DNA CEO Aaron Baker. "In addition, we have arranged preferred contractual arrangements with our premier affiliate network partners that waive affiliate network launch, integration and transaction fees during the migration process, and offer flexible contractual terms that match Google Affiliate Network terms."
Baker also noted that, unlike most of the top tier networks that are challenged with strained resources, DNA has the enormous bandwidth – and experience - needed to effectively perform multiple simultaneous migrations, as well as the in-house capabilities to instantly begin these migrations. The DNA team has handled over 1,000 migrations, including complex migrations for multiple premier brands and, as the leading affiliate management agency for premier brands, DNA has one of the most experienced and tenured teams in the industry.
"As a full service digital agency, DNA's hands-on, in-house team is poised to migrate Google Affiliate merchants to any of our partner networks, including Pepperjam Exchange, Commission Junction and Linkshare. We are able to support network migrations with a complete slate of management services, assuring a smooth, seamless and ultimately profitable transition for both advertisers and affiliates," said Samantha Morris, DNA's VP of Affiliate Marketing. "We see this as a terrific opportunity for impacted advertisers to turn a negative into a positive. We can clean up their existing programs as they move forward with key affiliate partners and because of our advanced business intelligence technologies, we can facilitate rapid new affiliate recruitment and program optimization enabling advertisers improved revenue and ROI, and affiliates to achieve greater levels of revenue than ever before."
DNA, which has offices in Pennsylvania, New York City and Raleigh, NC, was founded by, and is staffed, with a team of veterans from Top Digital Agencies. The DNA team has helped hundreds of advertisers – from start-ups to Fortune 500 brands - manage the most complex online marketing programs in the world. Dedicated to putting talent, research, technology and proven strategies at the forefront of its business, DNA offers unique pricing models to metrically-align its partnerships with advertisers.
DNA's complimentary migration program will only be offered until May 31, 2013 and requires the engagement of DNA's affiliate management services. The program will include:
- Migration to the affiliate partner network platform of choice
- Hands-on network migration execution and client support from the DNA team
- Preferred agency pricing with top tier networks which may include:
- Waiver of launch and integration fees
- Waiver of transaction fees during the migration process
- Contractual and pricing match with current Google Affiliate Network arrangement
- Brand protection to ensure client trademarks aren't being abused by affiliates within the search engines.
- One-of-a-kind recruitment approach so clients can see which affiliates are working with their competitors.
Interested merchants can contact DNA for a network landscape showing the benefits of each network at [email protected] or by calling 1.866.DNA.1275 x706.
About DNA
Digital Net Agency, Inc. (DNA) is a leading performance-based digital agency offering a full slate of services including Affiliate Management, Search Engine Optimization, Search Engine Marketing, Social Media, Brand Protection, Brand and Website Development, and CRM Management. Founded by a team of veteran search and affiliate marketing experts from Top 10 digital agencies, DNA is dedicated to partnering with clients to fuel growth while delivering maximum accountability through its performance-based models. DNA is based in Dallas, PA, with offices in New York City and Raleigh, NC.
Media Contact:
Melanie Webber, mWEBB Communications, 424-603-4340, [email protected]
SOURCE Digital Net Agency, Inc.
SYS-CON Events announced today that 910Telecom will exhibit at the 19th International Cloud Expo, which will take place on November 1–3, 2016, at the Santa Clara Convention Center in Santa Clara, CA. Housed in the classic Denver Gas & Electric Building, 910 15th St., 910Telecom is a carrier-neutral telecom hotel located in the heart of Denver. Adjacent to CenturyLink, AT&T, and Denver Main, 910Telecom offers connectivity to all major carriers, Internet service providers, Internet backbones and ...
Oct. 22, 2016 04:00 PM EDT Reads: 3,578
In his general session at 18th Cloud Expo, Lee Atchison, Principal Cloud Architect and Advocate at New Relic, discussed cloud as a 'better data center' and how it adds new capacity (faster) and improves application availability (redundancy). The cloud is a 'Dynamic Tool for Dynamic Apps' and resource allocation is an integral part of your application architecture, so use only the resources you need and allocate /de-allocate resources on the fly.
Oct. 22, 2016 04:00 PM EDT Reads: 3,645
In the next five to ten years, millions, if not billions of things will become smarter. This smartness goes beyond connected things in our homes like the fridge, thermostat and fancy lighting, and into heavily regulated industries including aerospace, pharmaceutical/medical devices and energy. "Smartness" will embed itself within individual products that are part of our daily lives. We will engage with smart products - learning from them, informing them, and communicating with them. Smart produc...
Oct. 22, 2016 03:45 PM EDT Reads: 1,440
More and more brands have jumped on the IoT bandwagon. We have an excess of wearables – activity trackers, smartwatches, smart glasses and sneakers, and more that track seemingly endless datapoints. However, most consumers have no idea what "IoT" means. Creating more wearables that track data shouldn't be the aim of brands; delivering meaningful, tangible relevance to their users should be. We're in a period in which the IoT pendulum is still swinging. Initially, it swung toward "smart for smar...
Oct. 22, 2016 03:45 PM EDT Reads: 629
In his keynote at 19th Cloud Expo, Sheng Liang, co-founder and CEO of Rancher Labs, will discuss the technological advances and new business opportunities created by the rapid adoption of containers. With the success of Amazon Web Services (AWS) and various open source technologies used to build private clouds, cloud computing has become an essential component of IT strategy. However, users continue to face challenges in implementing clouds, as older technologies evolve and newer ones like Docke...
Oct. 22, 2016 03:30 PM EDT Reads: 2,241
WebRTC sits at the intersection between VoIP and the Web. As such, it poses some interesting challenges for those developing services on top of it, but also for those who need to test and monitor these services. In his session at WebRTC Summit, Tsahi Levent-Levi, co-founder of testRTC, reviewed the various challenges posed by WebRTC when it comes to testing and monitoring and on ways to overcome them.
Oct. 22, 2016 03:00 PM EDT Reads: 3,882
In his keynote at 18th Cloud Expo, Andrew Keys, Co-Founder of ConsenSys Enterprise, provided an overview of the evolution of the Internet and the Database and the future of their combination – the Blockchain. Andrew Keys is Co-Founder of ConsenSys Enterprise. He comes to ConsenSys Enterprise with capital markets, technology and entrepreneurial experience. Previously, he worked for UBS investment bank in equities analysis. Later, he was responsible for the creation and distribution of life sett...
Oct. 22, 2016 02:30 PM EDT Reads: 5,218
SYS-CON Events announced today that Coalfire will exhibit at the 19th International Cloud Expo, which will take place on November 1–3, 2016, at the Santa Clara Convention Center in Santa Clara, CA. Coalfire is the trusted leader in cybersecurity risk management and compliance services. Coalfire integrates advisory and technical assessments and recommendations to the corporate directors, executives, boards, and IT organizations for global brands and organizations in the technology, cloud, health...
Oct. 22, 2016 02:15 PM EDT Reads: 1,495
@ThingsExpo has been named the Top 5 Most Influential M2M Brand by Onalytica in the 'Machine to Machine: Top 100 Influencers and Brands.' Onalytica analyzed the online debate on M2M by looking at over 85,000 tweets to provide the most influential individuals and brands that drive the discussion. According to Onalytica the "analysis showed a very engaged community with a lot of interactive tweets. The M2M discussion seems to be more fragmented and driven by some of the major brands present in the...
Oct. 22, 2016 01:45 PM EDT Reads: 11,261
The Internet of Things (IoT), in all its myriad manifestations, has great potential. Much of that potential comes from the evolving data management and analytic (DMA) technologies and processes that allow us to gain insight from all of the IoT data that can be generated and gathered. This potential may never be met as those data sets are tied to specific industry verticals and single markets, with no clear way to use IoT data and sensor analytics to fulfill the hype being given the IoT today.
Oct. 22, 2016 12:45 PM EDT Reads: 2,323
SYS-CON Events announced today that Transparent Cloud Computing (T-Cloud) Consortium will exhibit at the 19th International Cloud Expo®, which will take place on November 1–3, 2016, at the Santa Clara Convention Center in Santa Clara, CA. The Transparent Cloud Computing Consortium (T-Cloud Consortium) will conduct research activities into changes in the computing model as a result of collaboration between "device" and "cloud" and the creation of new value and markets through organic data proces...
Oct. 22, 2016 12:45 PM EDT Reads: 1,269
SYS-CON Events announced today that MathFreeOn will exhibit at the 19th International Cloud Expo, which will take place on November 1–3, 2016, at the Santa Clara Convention Center in Santa Clara, CA. MathFreeOn is Software as a Service (SaaS) used in Engineering and Math education. Write scripts and solve math problems online. MathFreeOn provides online courses for beginners or amateurs who have difficulties in writing scripts. In accordance with various mathematical topics, there are more tha...
Oct. 22, 2016 12:30 PM EDT Reads: 923
@ThingsExpo has been named the Top 5 Most Influential Internet of Things Brand by Onalytica in the 'The Internet of Things Landscape 2015: Top 100 Individuals and Brands.' Onalytica analyzed Twitter conversations around the #IoT debate to uncover the most influential brands and individuals driving the conversation. Onalytica captured data from 56,224 users. The PageRank based methodology they use to extract influencers on a particular topic (tweets mentioning #InternetofThings or #IoT in this ...
Oct. 22, 2016 11:00 AM EDT Reads: 8,270
SYS-CON Events announced today that SoftNet Solutions will exhibit at the 19th International Cloud Expo, which will take place on November 1–3, 2016, at the Santa Clara Convention Center in Santa Clara, CA. SoftNet Solutions specializes in Enterprise Solutions for Hadoop and Big Data. It offers customers the most open, robust, and value-conscious portfolio of solutions, services, and tools for the shortest route to success with Big Data. The unique differentiator is the ability to architect and ...
Oct. 22, 2016 10:15 AM EDT Reads: 587
SYS-CON Events announced today that Niagara Networks will exhibit at the 19th International Cloud Expo, which will take place on November 1–3, 2016, at the Santa Clara Convention Center in Santa Clara, CA. Niagara Networks offers the highest port-density systems, and the most complete Next-Generation Network Visibility systems including Network Packet Brokers, Bypass Switches, and Network TAPs.
Oct. 22, 2016 09:30 AM EDT Reads: 1,275
SYS-CON Events announced today that Embotics, the cloud automation company, will exhibit at the 19th International Cloud Expo, which will take place on November 1–3, 2016, at the Santa Clara Convention Center in Santa Clara, CA. Embotics is the cloud automation company for IT organizations and service providers that need to improve provisioning or enable self-service capabilities. With a relentless focus on delivering a premier user experience and unmatched customer support, Embotics is the fas...
Oct. 22, 2016 09:15 AM EDT Reads: 745
In an era of historic innovation fueled by unprecedented access to data and technology, the low cost and risk of entering new markets has leveled the playing field for business. Today, any ambitious innovator can easily introduce a new application or product that can reinvent business models and transform the client experience. In their Day 2 Keynote at 19th Cloud Expo, Mercer Rowe, IBM Vice President of Strategic Alliances, and Raejeanne Skillern, Intel Vice President of Data Center Group and ...
Oct. 22, 2016 09:15 AM EDT Reads: 1,421
Virgil consists of an open-source encryption library, which implements Cryptographic Message Syntax (CMS) and Elliptic Curve Integrated Encryption Scheme (ECIES) (including RSA schema), a Key Management API, and a cloud-based Key Management Service (Virgil Keys). The Virgil Keys Service consists of a public key service and a private key escrow service.
Oct. 22, 2016 08:30 AM EDT Reads: 951
Data is the fuel that drives the machine learning algorithmic engines and ultimately provides the business value. In his session at Cloud Expo, Ed Featherston, a director and senior enterprise architect at Collaborative Consulting, will discuss the key considerations around quality, volume, timeliness, and pedigree that must be dealt with in order to properly fuel that engine.
Oct. 22, 2016 08:15 AM EDT Reads: 3,779
Fifty billion connected devices and still no winning protocols standards. HTTP, WebSockets, MQTT, and CoAP seem to be leading in the IoT protocol race at the moment but many more protocols are getting introduced on a regular basis. Each protocol has its pros and cons depending on the nature of the communications. Does there really need to be only one protocol to rule them all? Of course not. In his session at @ThingsExpo, Chris Matthieu, co-founder and CTO of Octoblu, walk you through how Oct...
Oct. 22, 2016 07:45 AM EDT Reads: 3,108


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Discover a selection of top forums take admin on the internet. Come to share your passion on forums take admin and meet other fans of take admin. Then, why don't you create your own forum of take admin ?
Free Multi-Canine Roleplay: Come roleplay with us! We take any canine from foxes, wolves, dogs, and dire wolves, and everything in between. Join the good Society of Strays and sign up for the evil Mononoke Militia as well and let the battle begin!
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A demon has become interested in a human, and he won't take no for an answer.
The purpose of this forum is for the promotion and networking of fighting game enthusiasts and social gaming enthusiasts alike to gather and discuss issues relevant to them. Take care and enjoy! Removed on: 18/12/2014
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Temp Forums for Wolfhome Admins only.
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PewDiePieTalks is a place for Pewds fans to talk about the games Pewds play and talk about his youtube channel, Admin will be handed over to PewDiePie himself
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On the rise of a better faction, built just for you...our RisingSun Faction members. Engage yourself in the dream of a Perfect World with RisingSun at the lead. Of course that will take your skill, knowledge and participation in helping all your cl
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Area 52 is a fictional location and role play game. You can take the role of a Scientist, airman or Air Force Officer, or a Medical Doctor.
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In a Alternative world than ours, humans once existed but are a distant memory now as wolves take up the reign. Like humans, war is not uncommon.
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This forum is used to place suggestions, admin applications, let applications(non-admin position), and to alert us of any bugs that you may have found.
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Free forum : Warriors: The New Clans is a new take on the old Warriors series with five brand new, fan made clans and a fan-driven plot.
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Severity: Notice
Message: Undefined index: user_name
Filename: controllers/ideas.php
Line Number: 207
A huge moon, with a three-coned volcano which can be seen from the planet when it erupts and is taken for an omen. The planet, smaller than the moon, orbits it, and the planet in turn is orbited by a very small but very hot and bright ball of flame.
Far to the north amidst the endless ice-flows it is rumored that a dwarven Walrus Totem clan exists. These rumors have been unsubstantiated to date, unless the dwarven sage Glurt Goblinguts is to be believed. He claims to once having encountered a troupe of huge dwarves, each standing a beard's length higher than the tallest known dwarf. These dwarven "giants", their hairy chins crusted with frozen shards of ice and dirt, hauled gargantuan yellowed horns or tusks upon their wide shoulders, and their helms, likewise, sported massive, down-ward pointing tusk-horns. Glurt Goblinguts later speculated that the impressive size of these dwarves was most likely due to their arctic diets, almost exclusively fat-based.


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Tas indicators
|September 22nd, 2013, 06:47 AM
|#1 (permalink)
Elite Member
Pretoria South africa
Futures Experience: Intermediate
Platform: eSignal
Favorite Futures: Oil
Posts: 4 since Sep 2013
Thanks: 0 given, 0 received
Tas indicators
Are anybody using TAS indicators? I came across nice setup where MA cross Tas box and price bar simultaneously> Whish I can activate an alarm for this! See example of yellow and blue arrows.


text:
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Hotels in Almagro
Average price per hotel night and category at Almagro for the following weeks
|2 hotel
|3 hotel
|4 hotel
|10/24/16 - 10/30/16
|2 hotel €35
|3 hotel €34
|4 hotel €55
|10/31/16 - 11/6/16
|2 hotel €34
|3 hotel €30
|4 hotel €56
|11/7/16 - 11/13/16
|2 hotel €40
|3 hotel €34
|4 hotel €49
|11/14/16 - 11/20/16
|2 hotel €35
|3 hotel €31
|4 hotel €46
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Hotels by category
Points of Interest in Almagro
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