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Strecker, George J., Jean-Pierre Wuarin, and F. EdwardDudek. GABAA-mediated local synaptic pathways connect neurons in the rat suprachiasmatic nucleus. J. Neurophysiol. 78: 2217–2220, 1997. The suprachiasmatic nucleus (SCN) in mammals functions as the biological clock controlling circadian rhythms, but the synaptic circuitry of the SCN is largely unexplored. Most SCN neurons use the neurotransmitter γ-aminobutyric acid (GABA), and anatomic studies indicate many GABAergic synapses and local axon collaterals; however, physiological evidence for synaptic communication among SCN neurons is indirect. We have used three approaches to investigate local circuitry in the SCN in acute hypothalamic slices from rat. First, tetrodotoxin was used to block action-potential-dependent synaptic release, which resulted in a decrease in the frequency of spontaneous synaptic currents in SCN neurons, suggesting that spontaneously active neurons in the slice connect synaptically to SCN neurons. Postsynaptic currents in SCN neurons were also evoked by the selective stimulation of other SCN neurons with glutamate, which avoids direct activation of axons that might originate outside the SCN. Two different methods of glutamate microapplication (i.e., pressure ejection and ultraviolet photolysis of caged glutamate) indicated that SCN neurons receive GABAA-receptor-mediated synaptic input from other SCN neurons. In contrast, glutamate-receptor-mediated synaptic connections between SCN neurons were not detected. The GABAergic synapses that comprise the network described here could conceivably be a substrate for the synchronization and amplification of the circadian rhythm of SCN firing. Alternatively, this circuitry might mediate other aspects of clock function such as the integration of environmental and physiological information.
Numerous neuroactive substances have been detected in the suprachiasmatic nucleus (SCN), but only γ-aminobutyric acid (GABA)- and glutamate-receptor-mediated postsynaptic events have been reported (see Strecker et al. 1995, for review). It is unknown whether these events originate from local neurons or from neurons located outside the SCN. The SCN contains extensive GABAergic terminals andsomata (van den Pol 1986), and Golgi studies have revealed local axon collaterals of SCN neurons (van den Pol 1980). With anatomic evidence alone, however, it is difficult to know whether SCN neurons actually form functionalGABAergic synapses with each other. Physiological experiments to probe synaptic connectivity have typically utilized electrical stimulation, but this approach could also activate axons originating outside the SCN. In the present study, glutamate was used to stimulate neurons without activating axons. The postsynaptic currents (PSCs) evoked by glutamate microstimulation in the SCN provide physiological evidence for the presence of GABAA-receptor-mediated synaptic pathways between SCN neurons.
Sprague-Dawley rats were housed under a 12-h light:dark cycle and recordings were made predominantly during the light phase. Coronal slices (150–400 μm) of hypothalamus were cut and maintained in artificial cerebrospinal fluid (ACSF) containing (in mM) 125 NaCl, 2.5 KCl, 1 CaCl2, 1 MgCl2, 24 NaHCO3, 1.25 NaH2PO4, and 10 glucose, bubbled with 5% CO2-95% O2, pH 7.4. Recordings were made at room temperature (20–22°C) from 2- to 4-week-old rats.
Patch pipettes (3–5 MΩ) contained (in mM) 140 cesium gluconate (or 130 KCl in photostimulation experiments), 1 CaCl2, 1 MgCl2, 1 NaCl, 5 bis-(o-aminophenoxy)-N,N,N′,N′-tetraacetic acid, or 5–10 ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid, and 2–4 magnesium ATP, pH 7.2. Whole cell currents were amplified, filtered (corner frequency 5 kHz, Axopatch 1D), and stored digitally. Recordings were refiltered (1 or 2 kHz), redigitized (5 kHz), and analyzed off-line (pClamp software).
The frequencies and amplitudes of spontaneous PSCs were measured before and after the addition of tetrodotoxin (TTX) to block action-potential-dependent synaptic activity. Cumulative amplitude distributions of inhibitory PSCs (IPSCs) were compared with the use of the Kolmogorov-Smirnov test. The one-sample χ2 test was used to assess the effect of TTX on IPSC frequency. Data are generally expressed as means ± SE.
In TTX and glutamate microapplication experiments, 150- to 200-μm slices were submerged in ACSF and SCN neurons were recorded while being viewed with Nomarski water-immersion optics (Edwards et al. 1989). Glutamate (1–2 mM; 5–10 mM in initial experiments) in ACSF was applied by pressure pipette (∼0.2 s) onto the SCN during recording. Photostimulation experiments were performed in 400-μm-thick slices with the use of the blind-patch technique (Blanton et al. 1989). Slices were submerged in ACSF containing 250–500 μM caged glutamate [γ-(α-carboxy-2-nitrobenzyl) ester, trifluoroacetate; Molecular Probes]. Flashes of ultraviolet light (xenon lamp; duration ∼ 0.5 ms) were focused from beneath the slice into the SCN to uncage glutamate (Callaway and Katz 1993).
Data from neurons that showed clear synaptic responses evoked by glutamate microstimulation were analyzed for both magnitude and repeatability of the effect of glutamate on IPSC frequency. Cells that did not show clear responses were counted as unresponsive and not analyzed further. Magnitude was assessed by statistically comparing (1-sample χ2 test) the number of IPSCs occurring 10 s before and 10 s after the stimulus in the case of microapplication. In the photostimulation trials, the duration of the effect was shorter because stimulation was more focal, so a shorter poststimulus interval (2–8 s) was examined.
To assess repeatability, the binomial theorem was applied to evaluate whether any series of sequential trials displayed significantly more positive responses than were likely by chance (P < 0.05). The probability of positive or negative responses was assumed conservatively to be equal (i.e., 0.5). For evaluating a series of trials, a trial with a positive response was defined as having more (P < 0.2, χ2 test) IPSCs after stimulation. Positive responses so defined showed evoked increases in IPSC frequency by a factor of 2.4 ± 0.2 (mean ± SE; n = 81 positive responses) with microapplication, or a factor of 2.6 ± 0.2 (n = 30) with photostimulation. Some cells appeared clearly responsive, but not in enough consecutive trials to achieve significance of repeatability. In these cases, IPSC increases in individual trials were held to more stringent levels of significance (P < 0.05 rather than 0.2, χ2 test). Thus we defined responsive cells as having either 1) a binomially significant series of responses, with each response at the P < 0.2 level, or 2) at least three consecutive responses, each at the P < 0.05 level. Our approach is likely to underestimate the extent of local interactions in the SCN, but provides a reliable minimum value.
Spontaneous IPSCs were detected in 151 of 152 SCN neurons. Bath application of bicuculline (10 μM) blocked spontaneous IPSCs in 10 of 10 neurons, confirming that the IPSCs were mediated by GABAA receptors. Spontaneous excitatory PSCs (EPSCs) were much less frequently detected (i.e., 15 of 47 SCN neurons).
A dependence of spontaneous IPSCs on action potentials would suggest that active presynaptic neurons were contained in the slice, possibly within the SCN itself. The mean frequency of spontaneous IPSCs in control solutions was 17.7 ± 14.4 Hz (n = 7 neurons). In each cell, the addition of TTX (1–3 μM) significantly reduced the frequency of IPSCs (P < 0.005, χ2 test, n = 7) to 44 ± 8% of the pre-TTX frequency (Fig. 1). Cumulative amplitude distributions of spontaneous IPSCs revealed a significant reduction by TTX in two of seven cells (Kolmogorov-Smirnov 2-sample test, 1-tailed). These results suggest that at least some spontaneously firing neurons in the slice formed inhibitory synapses on the recorded SCN neurons, but these presynaptic neurons were not necessarily in the SCN.
To test for the presence of local synaptic connections more directly, we examined whether microapplication of glutamate to the SCN could induce GABA-receptor-mediated PSCs in SCN neurons. Unlike electrical stimulation, glutamate is expected to stimulate only somata and dendrites and not axons (Christian and Dudek 1988). Of 32 cells, 8 (25%) responded significantly and reproducibly with increases in IPSC frequency to glutamate microapplied by pressure ejection (Fig. 2). Four additional neurons (i.e., 12 of 32 cells, 38%) showed at least one trial with a significant (P < 0.05, χ2 test) increase in IPSC frequency. Glutamate microapplication failed to evoke EPSCs in 17 of 17 cells.
To improve the temporal and spatial characteristics of glutamate application, a second method was used—photolysis of caged glutamate. Of 12 SCN cells tested, 3 (25%) responded to glutamate uncaging inside the SCN with significant and reproducible increases in IPSC frequency (Fig. 3), and a 4th cell responded (P < 0.05 level) repeatedly but not consecutively enough to satisfy statistical reproducibility. Because of the enhanced temporal and spatial resolution with this method, we were able to observe one other neuron that repeatedly displayed a single IPSC 25 ms after each glutamate photolysis stimulus, rather than the burst of PSCs more commonly seen. As expected, bicuculline blocked all evoked IPSCs (n = 2 neurons). No EPSCs could be evoked in 11 of 11 neurons tested with photostimulation.
Experiments showing that TTX reduced the frequency and occasionally the amplitude of IPSCs indicate that at least some of the neurons responsible for GABAergic synaptic input onto SCN neurons were present in the slices, possibly within the SCN (see also Jiang et al. 1995). In these experiments it is assumed that cut axons lacking a soma or axon hillock are less likely to fire spontaneously compared with intact axons connected to SCN somata, which fire spontaneously at ∼10 Hz during the day.
Our experiments with glutamate microstimulation provide more specific evidence that local synaptic pathways exist in the SCN and are predominantly GABAergic. Only GABAA-receptor-mediated IPSCs, either spontaneous or locally evoked, were detected in SCN neurons. Spontaneous EPSCs were also detected, but no EPSCs could be locally evoked. In both the microapplication and photolysis experiments, 25% of neurons displayed local GABAergic input. This is likely to be a lower bound, given the stringency of our criteria for the identification of responsive cells and the partial deafferentation that is characteristic of slice preparations. It is unlikely that glutamate microapplication caused synaptic release by direct action through metabotropic receptors on presynaptic terminals. Such receptors in mammals are typically found to inhibit IPSCs (Desai et al. 1994; Jouvenceau et al. 1995; Llano and Marty 1995; Schrader and Tasker 1997).
The GABA-mediated synaptic network demonstrated here has important implications for SCN function. This network might be involved in the synchronization of the circadian rhythm of neuronal firing. SCN neurons fire at a higher rate during the day than at night (Inouye and Kawamura 1979). Although GABA is typically inhibitory, action potentials can be initiated in other regions by synaptically mediated postinhibitory rebound (see Llinás 1988 for review). GABA-mediated synaptic currents in the SCN also might lead to action potentials and synchronize neuronal activity. Recent evidence suggests that GABA excites SCN neurons during the day and inhibits them during the night, because of shifts in the chloride equilibrium potential (Wagner et al. 1997). Thus this network might be used to synchronize SCN neurons or to increase the amplitude of the circadian change in firing frequency. Alternatively, it might be involved in phase shifting or in the integration of environmental information rather than in the rhythm of firing per se.
We are grateful to Drs. Gary Pickard and Patricia Sollars for comments on this manuscript.
This work was supported by grants from the Air Force Office of Scientific Research to F. E. Dudek and the National Institute of Neurological Disorders and Stroke (R01 NS-32662 to J.-P. Wuarin).
Address reprint requests to F. E. Dudek.


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Horrible Online Shopping Experience!!!
Target Stinks!!! This morning I had the worst online shopping experience of my life!!! I Will never never never shop online or in their stores again!!! I was on the bridal registry shopping for multiple shower gifts. Got to the check out, and seen that I was charged a high rate shipping charge. I thought it was free shipping over 50.00. I thought I had cancelled that order. I went back to the registry, and looked at items purchased, it showed nothing had been purchased so I thought the order did not go through on my pay pal account. {NEVER REC'D AN ORDER NUMBER TO LET ME KNOW THAT IT WENT THROUGH AT THAT TIME}. I then did a re order, scaling back on items to bring down the shipping charges, only because my nephew and his bride to be are registered at Target in Denton County. Looked at my pay pal acct. I now have 2 orders, which they both had the blender they wanted. They will be getting two of. The cust. service supervisors said that they would put in a request for a cancellation for the first order that I thought was cancelled, but let me know that I am probably stuck with that order as well which is being shipped directly to the couple!! They did not want two blenders, only one. Now I am out shipping for two separate orders!!! They need to be turned in to the BBB for ripping people off!!! It seems like Target would want people to love shopping with them, not trying to rip off the consumer, and having to put up with their crappy customer service, and their lousy excuses as to why they can not cancel an order. They told me that I had 30 mins. to do so. WELL, THAT NEEDS TO BE STATED ON THAT REGISTRY, NOT IN AN EMAIL THAT I DID NOT KNOW ABOUT HOURS LATER!!!!!!!
A2Z store
The clearance items are relatively fashionable and worth taking a look at.
Great collection.
good customer service
I would definitely recommend.
A fast, easy way to shop.
Since I live very close by, I can walk to Target to buy groceries and other things very quickly. The main reason though is because I can find almost anything I need inside! Great store with great employees!
Target is my go to store
This store has almost everything I need. Cute clothing and accessories, food, electronics, and home goods. I go to target for almost every gifting opportunity, and to shop for myself :p
Get the best deals here for the Christmas gift!
Here you can get the best deals online!
All the items here are 50% off and free shipping! we are a whoelsaler of all kinds of ugg boots. To get the authentic ugg boots here at the cheapest price! Despite the free shipping offer and best discount, you can also get better deal if you can buy more than 3 pirs of boots. Order 3 item, save 5%. Order 4 items, save 6%. Order 5 items, save 8%. More than 5 items, Pls contact us, more discount will be given to you!
why i shop target
i shop target stores because of the merchandise offered. most are as good and stylish as any fine dept. store. also if i have a problem and want to return an item, even if i'm visiting another state they never give me a problem. i alwaays look in the sunday paper for your special sale booklet.
love the one stop shop
I work all week and my time is limted, so I love going to target where I can get all my shopping done. I can pick dinner, a card for birthday and gift, something to wear, this is great.
TGIF buffalo chicken nuggets excellent
dress bought for vacation 5 *****
bath towels 5*****
Always a good deal
Walmart may have a wider selection and overall better prices, but you can't rule out target when shopping for almost anything. From Televisions to hotdogs Target has almost anything you need. Often times they have the best price on many Dvds or electronics.
While it may not be as big as Walmart I am generally more satisfied after a trip to Target than I am with Walmart. They just have more niche items I like to shop for.
Target is definitely a hit!
Target is a fun store to shop in. I find a lot of nice clothes for my grandaughter here. The specials they have on the household items are usually a great buy. Target is a nice place to shop and grab a bite to eat at the concession stand.
neat and clean, bright,
I love Target. I must say, my husband prefers to go to Walmart for bargains, but I find I can get really good prices on certain items at Target. Recently they have been adding more of their own brand of products and I find them to be of the utmost quality. They get a nice cross section of shoppers and as crowded as they might be, the registers are all open to accomodate everyone. Also, the employees are very polite and helpful.
Good Assortment
Target leads the way in assortment and quality, especially in childrens clothes. Good prices,good value.
I have been going to target for a very long time so that must mean they are doing something right!I must admit that there has been a few times where an employee wan't the nicest but that is few and far in betweeen. I have usualyy found that the stores are organized and kept clean, the employees are helpful, the cashiers almost always are really nice and put a smile on my face. The prices for some things, I think, are a little high but the do have a lot of sales so it's ok. my cosmetics I usually like to get from them because theuy have a very good selection. all in all an A- Ok store.
Very good,but still room for improvement
I enjoy shopping our Super Target - bright & clean ennvironment, quality products displayed with eye appeal and fairly reasonable prices. However, since the economic down trend, staffing seems to be inadquate in number of sales associates and their training in customer service. I hope Target can raise the quality of their sales associates customer service training.
Great Prices and fair deals. I probably much shop at target every weekend.
Had purchased Rubbermaid Kitchen Storage Containers for a Christmas gift from Target because they had a pack that had a many sizes in and I couldn't find it anywhere else. The price was reasonable for what you got. Three days before Christmas, I received an e-mail confirmation stating it was not going to get to me on time due to the holiday rush and wouldn't receive it til the end of January. I was extremely upset considering I had ordered it more than a week prior to Christmas Eve and it stated the item was IN STOCK and guaranteed on-time delivery. I called customer service, who appologized, but I felt I got no where even after speaking to a manager....Long story short, my package arrived ON TIME!!! -- Target came through for me and I couldn't be happier! I am grateful for the outstanding job they did to satisfy me as a loyal customer. I will continue to shop Target as I love their products and am impressed with the job they did to satisfy my needs. THANK YOU!
A Store for All Needs!
All Targets that have transformed into Super Targets have been a blessing. Since Super Target opened, my grandmother and I have been able to save so much money. We have virtually stopped shopping at our "regular" grocery store ,and have been able to buy fresh fruit, bread ,and milk at huge discounts. We were starting to get worried , because the prices of Jewel - Osco were getting higher and higher and the quality of the food was dropping....there was no competition . We are very thankful that Super Target offers such wonderful values to it's customers!
had very nice shopping trip the employees were consideret and helpful :)
Still Cheap Chinese Crap
I find it funny that people who bash Wal-Mart for selling cheap, plastic Chinese crap feel good about shopping here. It's the same stuff in a slightly more hip package.
Great Variety!
I love the fact that Target always seems to have variety and a choice for every item I need.
I recently purchased a no contract phone from them and was pleased with the choices available and that they had everything in stock!
Nice Clean Store
I use target often. They have quality products and great prices. More importantly they have a clean appearance without the clutter in the isles that you see in other stores.
Great deals
The clearance items are relatively fashionable and worth taking a look at.
They have a great selection of jackets
Great selection of hoodies.
Overall it's a very clean and well taken Care of location
I would definitely recommend.
home decor
I love the home deco section. It is fun to go shopping there.
fun store
Taget is a fun store to shop in. Not much in the adult clothing line. Everything else is great. I love their dollar shelves when you first walk in. I always stop and take time to browes. I will find something to buy evertime I go.
While shopping at Target looking for a game console realizing all things were behind lock and key.Unable to try them and find a salesperson to help answer questions concerning them .I became frustrated and purchase item else where.
Favorite Place to Shop
Ever since I was a little kid, Target has been my favorite store. I live very close to one and I still look forward to going any chance I get. I loved the toy selection as a kid. Just looking at all of them was a blast and since the prices are so good my parents would buy me cool toys all the time. Now that I've grown, I still love to go to buy things for my little sister.
I also love the clothing department. The clothes are cool and classy. It suits my style very well. I even bought my favorite hat and my front-pocket wallet from there, both great purchases and under $10 each. The wallet is genuine leather!
Target is a fun place to go and most of them have a Starbucks and/or a good restaurant inside.
If you need a gift for someone, or even yourself, I strongly recommend Target.
More Target Reviews
Target Business Address / Contact Information
Target Corporation
1000 Nicollet Mall
Minneapolis, MN 55403
Online Shopping Support Number: 1-800-591-3869
Store Support Number: 1-800-440-0680
Online Gift Registry: 1-800-591-3869
Store Gift Registry: 1-800-888-9333
Phone Number: 612-304-6073
Fax Number: 612-696-5400
Stock: TGT (NYSE)
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text:
Rajinikanth wants Kamal Haasan to watch 'Kochadaiyaan'
The film is crucial for Rajinikanth as it would be his first after his hospitalization two years ago.
Mumbai: In what could be regarded as a major departure from the custom, Kamal Hassan is all set to view his friend and professional rival Rajinikanth's latest film, the long awaited 'Kochadaiyaan'. The film is crucial for Rajinikanth as it would be his first after his hospitalization two years ago. The film adopts a new form of storytelling known as the Motion Capture technique where all of the Tamil superstar's actions would be heightened and amplified through special effects.
Understandably, Rajini is said to be anxious and has asked Kamal Hassan, known to have a deep comprehension of cinema, to step in to suggest changes. Only then will Rajinikanth decide on a release date. "Rajini considers Kamal to be extremely cinema literate. He respects Kamal's judgement on cinema. Since this film is crucial (for him), he has asked Kamal to view the film," said a source from Chennai.
A suitable date for the screening is being arranged. Speaking fondly about his friendship and rivalry with Rajinikanth, Kamal says "I'd do anything he asks me to, no question of refusing him. Both of us were young actors who started from scratch. He was the bus conductor with acting aspirations. I was the clapper boy who got the clap in my very film (Kamal Haasan played a character with venereal disease in his first adult role).
"We didn't have fathers to make movies for us. But we had the same godfather K Balachander. We've done around 10 films together, most of them were hits, though we did have failures. We've done three films for our mutual mentor Balachander," he added. Kamal also says he can never say 'no' to Rajinikanth.
"I've done guest roles in Rajini's films. It's quite strange, but our paths as actors were always intertwined. Initially we were both typecast as villains. I got fed up doing those and went away to Kerala to do Malayalam films. Then his career as a villain took off, and I returned to Chennai to pursue a career in Tamil cinema.
"We made our way up together. Rajini once pointed out that though he's four years my senior, I'm his senior professionally and that he would take my advice about doing a film with me, if there's an occasion for us to star together," he added. Kamal doesn't rule out the possibility of doing another film with Rajinikanth.
"It depends. He said if we do a film together, he won't direct it. It has to be either me or someone else. It would be quite interesting to do a film with him," he said.
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text:
Welcome to Librarium Online!
Join our community of 80,000+ members and take part in the number one resource for Warhammer and Warhammer 40K discussion!
Registering gives you full access to take part in discussions, upload pictures, contact other members and search everything!
I'm sure I'm not the first to have thought of or to have tried this; its a way to get the most out of all the parts included with your Destroyer Lord. I've used magnets to allow me to switch back and forth between the weapons and wargear options that come in the D Lord box.
I used a 1/16" magnets and a 1/16" drill bit to make the seat for the magnets. They are held in place with super glue.
Here are the arm options with the magnets in place.
Here are the weapon options (Scythe and SoL).
I had to use two magnets due to the extra weight.
Here are the two sides of the Destroyer. You can see where I placed the magnets to coorespond to the magnets on the weapons. You have to make sure you keep the + and - in mind or the magnets will repell the weapons instead of holding them. I marked the one side of each magnet with a marker to keep things straight.
The other side only needed one magnet as the non-weapon arms are lighter and can be held in place with just one.
Here is the same D Lord model with different weapon and wargear configurations.
I plan on making another lord on foot and putting magnets in his arm sockets so they can share parts. This was a very easy modification to the Destroyer Lord. I was suprise how well things fit together and how quickly I finished this process (it only took about 45 minutes). As you can see from the pics, the magnets are almost invisible. This will save us from having to buy extra models just to have different weapons configurations available. Happy Modeling!
Not that the lord has many weapon configurations anyway....
Still that is some nice work, it at least makes it easier if you want to change the arms around for coolness.
It's not just for coolness. I don't know about your gaming group be we are hardcore about WYSIWYG. If it's not on the model, you don't have it, even if you paid the points. This configuration allows me to use any of the options included in the Destroyer Lord box. I didn't have to choose between the Scythe or SoL or, weather or not he'd be carrying a Res Orb. I can just swap out arms at any time to suit any need.
I'm actually doing the same thing with my Heavy Destroyers... I magnetize both the gauss cannon and heavy gauss cannon and switch as necessary. Altough I dont dril the magnets, I just superglue them.
This is a great idea!Originally Posted by boreas
i wish id thought of it, my guys have both weapons glue on and its not exactly something GW likes.
In the name of god impure souls of the living dead shal be banished into eturnal damnation. Amen.
Wow... that is really, really cool (and useful!). If only I didn't already build my lords. Kudos man, it looks great.


text:
River interlinking on large scale not practical: Murli Manohar Joshi
Joshi said, had it been practical, then nature itself would have done it. "The rivers would have been inter-connected. But it is not."
Need for interplay between public opinion, lawmakers: Murli Manohar Joshi
"The rule of law is not confined with rule by law as it may become ...
IITs, IIMs in bad position: Murli Manohar Joshi
Former HRD minister also suggested that the government should assess the country's technologi...
Amit Shah to meet L K Advani, Murli Manohar Joshi for 'blessings'
Shah, who has been elected for a three-year term, also will be meeting ailing former Prime Minister Atal Bihari Vajpayee on Tuesday, party sources said.
Murli Manohar Joshi, LK Advani stay off festivities as Amit Shah gets re-elected
Sources close to Advani said the senior leader decided to stay away since he thought Shah's election was a mere formality and there was no point being a party to it.
Secularism of Congress, Left a ruse for hating Hindutva: Murali Manohar Joshi
The people had rightly dumped them "at the dustbin of history", Joshi said addressing a public meeting organised by BJP's party unit in Bengaluru.
LK Advani, other BJP veterans like Murli Manohar Joshi, Shanta Kumar get into a huddle
Sources said Advani drove down to Joshi's residence and Shanta Kumar and Sinha joined them at noon for a meeting that lasted about an hour.
Murali Manohar Joshi not invited at BJP National Executive meet
Some party workers have put up posters expressing their protest against what they see as an insult of a senior leader.
Manohar Parrikar to inaugurate copter maintenance plant tomorrow
The plant has been set up at Honda village, where the company had purchased assets from the state government-run Economic Development Corporation (EDC).
Congress leader Rita Bahuguna Joshi to join BJP?
Congress may face further desertions in its ranks ahead of Uttar Pradesh Assembly elections with former state president and MLA Rita Bahuguna Joshi likely to join Bharatiya Janata Party.
Lal Krishna Advani, Murli Manohar Joshi attend BJP parliamentary party executive meeting
Advani and Joshi are among the members of the group which meets to formulate the party's strategy during a parliamentary session.
Wealth creation ideas by Sharmila Joshi
Watch Sharmila Joshi, sharmilajoshi.com, for wealth creation ideas by investing in stock market.
Manohar Parrikar credits RSS for surgical strikes
"May be the RSS teaching was at the core, but this was quite a different kind of combination," Parrikar said in remarks that may trigger a row.
LK Advani, Murli Manohar Joshi, others meet; some feel elders waiting for opportune moment to hit out against party leadership
Advani, Joshi, Shanta Kumar and Sinha's meeting fuelled speculation of fresh troubles in the party, given their Diwali eve huddle had created a political storm.
Bihar poll debacle: Full statement by LK Advani, Murli Manohar Joshi, Shanta Kumar and Yashwant Sinha
" It shows that those who would have appropriated credit if the Party had won are bent on shrugging off responsibility for the disastrous showing in Bihar."


text:
Epson 87 Orange Ink Cartridge (T087920)Ink Cartridges & Printheads
OEM Epson Ink Cartridge - 915 Page Yield - Orange - View Complete Details
Product Number: EPST087920
In Stock - Hurry, only 102 left.
Estimated Delivery - 1 business day(s) - Tue 10/25 2 business days - Tue 10/25
Shipping - This item is eligible for Free Shipping on orders over $45
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Product Details
Not Returnable
Due to its limited shelf life or other concerns, we do not accept returns on this product unless it is damaged, defective or incorrect due to an error on our part.
Epson 87 Orange Ink Cartridge (T087920) - Ink cartridge is designed for use with the Epson Stylus Photo R1900. Engineered to give you optimum photographic output. Provides superior resistance to water, fading and smudging.
- Create brilliant archival prints and design projects.
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Replacement ink cartridge is designed for the Epson Stylus Photo 2200. UltraChrome pigment inks provide superior resistance to water, fading and smudging. The wide color gamut allows truer, more realistic pictures.Pigment-based ink.. Expanded color gamut.. Unique package design.
Produce durable prints with superior image quality that are smudge-, water- and fade-resistant by using DURABrite™ ink. Convenient individual cartridges so you can replace only those needed. Quick-drying formula for worry-free handling of photos.DURABrite™ ink is smudge-, water- and fade-resistant.. ...
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Facebook is a popular website for keeping in contact with friends, colleagues, and family, as well as networking with people anywhere in the world who share your interests. Your Facebook page includes a built-in chat client that allows you to chat with Facebook friends who are online. Facebook has no built-in method for saving chat text, so if you wish to save the text of a Facebook chat, you must copy and paste the text to a word-processing application.
Navigate your web browser to www.facebook.com and click the "Chat" tab in the bottom-right corner of the page to open a list of friends who are online. Click on a friend's name to open a new chat window and begin to chat.
Click and drag to highlight chat text you wish to copy.
Right-click the highlighted text and click "Copy" in the popup menu. The text will be copied to your computer's internal clipboard.
Open Microsoft Word, Notepad, or any other word-processing application.
Right-click the application's program window and click "Paste" in the popup menu. Your Facebook chat will be pasted into the application.
Tips & Warnings
- Each line of chat text is preceded by two lines of information. The first line contains the name of the person who wrote the message, and the second line contains a time stamp indicating when the message was sent.
- Photo Credit Jupiterimages/Comstock/Getty Images
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text:
Revolymer PLC (REVO) Insider Buys £14,400 in Stock
Revolymer PLC (LON:REVO) insider James Joseph Barber acquired 45,000 shares of the company's stock in a transaction on Wednesday, October 5th. The shares were bought at an average cost of GBX 32 ($0.42) per share, with a total value of £14,400 ($18,796.50).
Revolymer PLC (LON:REVO) opened at 35.00 on Thursday. Revolymer PLC has a one year low of GBX 27.00 and a one year high of GBX 82.00. The company's market cap is GBX 20.26 million. The company has a 50 day moving average of GBX 37.36 and a 200-day moving average of GBX 43.01.
Separately, Panmure Gordon reissued a "buy" rating and set a GBX 70 ($0.91) price objective on shares of Revolymer PLC in a report on Monday, September 19th.
Revolymer PLC Company Profile
Revolymer plc is engaged in the specialty chemicals business focused on controlled release, responsive systems and delivery systems. The Company operates in both fast moving consumer goods (FMCGs) markets, such as home care, as well as other markets, such as personal care and nicotine gum. It operates through the gum segment, which includes both confectionery and nicotine.
Receive News & Stock Ratings for Revolymer PLC Daily - Enter your email address below to receive a concise daily summary of the latest news and analysts' ratings for Revolymer PLC and related stocks with our FREE daily email newsletter.


text:
Interdependence Project Executes Day of Action.
I entered the Interdependence Project (IDP) meeting at their Bowery location on No Impact Day with a sense of anticipation. IDP joined an initiative to rid New York City of plastic bags. As someone who has been following them online, I was glad to see that they were hosting events on the weekend when I was visiting New York City for my sister's birthday. As we went around the room sharing one word about how we felt, I was comforted to learn that I wasn't the only one who felt anxious, excited and curious.
IDP hosted this event in collaboration with Colin Beavan, a.k.a. No Impact Man. Colin emphasized that it was the folks in the room who organized the day of action and not him. He stressed that we shouldn't be attached to particular outcomes, but should pay attention to how people react to us and how we react to the experience. After he got things rolling, he handed things over to the group and left the room.
The participants included a range of experience in social service and activism. One woman who works at an alternative school considered how she could get her students involved. She wanted to win and she was eager to get out and take action instead of just talking about it. Another woman shared a wealth of experience from Green Peace organizing. My partner turned out to work in development at an old settlement house.
There was a certain vitality and freshness in the room. There was open space for everyone's contribution. I shared some ideas from my experience canvassing against George W. Bush in 2004 and people welcomed them, even though I plopped in after they had been preparing for at least a week.
We came up with last-minute strategy tweaks to the existing plan of going out in pairs of two and requesting store clerks and managers to consider switching from automatically offering customers plastic bags to asking first if they are needed. If appropriate, we asked if the store would consider taking steps towards eliminating plastic bags altogether- a major campaign embraced by IDP.
Hitting the streets
The fear and anxiety was quickly transformed into exhilaration and with every uninterested or hostile comment, I was ready to learn, smile and move on, just like my days canvassing. The first woman we talked to (pictures) was friendly and sympathetic and invited us to leave fliers.
Coming back, we shared our experiences. A few people's descriptions really reminded me of the ethos of what we call a Bearing Witness Plunge at the Zen Peacemakers. Colin noted that we developed a palpable conviviality between us. Pictured below: No Impact Day participants including IDP organizer Patrick Groneman. Thanks IDP!
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Opening of the 61st Cannes Film Festival
In an atmosphere of high expectations of new cinematic
discovery today in the south of France starts 61st International
Cannes Film Festival. He completed 24 May awarding winning films
Film Forum's highest award - the Palme d'Or.
61st Cannes Film Festival opens tonight show mystical thriller "Blindness" Brazilian Fernando Meyrleysa. It is expected that the film will be one of the highlights of the festival.
In the main competition program includes 22 feature-length films. This year, among them there are no Russian film, reports Radio "Mayak».
However, this time it was in Cannes, it was decided to celebrate the 100th anniversary of Russian cinema and the 50th anniversary of our single "Palme d'Or", which won the painting "The Cranes Are Flying».
Russia's main competition there, but never as much of Latin America. Get at least the movie "Discovery". The festival will open today the Brazilian director Fernando Meireles with the movie "Blindness". However, the picture is officially Canada, and the actors Julianne Moore and Gael Garcia Bernal speak English and Japanese.
The competition involved recognized masters, not just to win in Cannes, for example, Jean-Pierre and Luc Darden, Steven Soderbergh and Clint Eastwood. Last picture called "Changeling" with Angelina Jolie in the title role critics have called the favorite festival before viewing.
This year's festival jury headed by the famous American actor Sean Penn. The winner will be announced on May 24.
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Cannes Film Festival 2014: Ball amfAR
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My Dad Will Do
Written by Martin Thomas
Illustrated by Ag Jatkowska
Lovereading4kids Price £5.59
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The Lovereading4Kids comment
This child's-eye view of the father-child relationship is very touching and will strike a chord in all families. Little elephant looks up to Dad – literally – and each spread describes something Dad can do because he's bigger, whether that's making dinner, going to the shops or just hanging up their coats. The simple rhyming text, supported by warm, cosy illustrations, captures the sense of love between parent and child, and on one of the final spreads Dad kneels down low so that little elephant can hug and hold him tight, 'when he is short and I'm his height'. ~ Andrea Reece
My Dad Will Do by Martin Thomas
It's not easy being small. Especially not being able to reach things. Like the hook to hang a coat, a shelf to get the toys, or the pedals of a bike. Kitchen sides, the shower, and a light switch are all too high. But the love of a father is an amazing thing. With dad close by anything is possible. With dad close by nothing is out of reach.
About the Author
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Martin Thomas
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Wacky Bee Books
Publication date
1st February 2016
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American Heart Association Youth Market Director - Alachua, Marion (+) Counties in Gainesville, Florida
Youth Market Director - Alachua, Marion (+) Counties
Tracking Code
Job Description
Are you ready to join an organization where you can make an extraordinary impact everyday?
Imagine all Americans enjoying ideal cardiovascular health free of heart disease and stroke. At the American Heart Association and American Stroke Association, we get to work toward that goal every day. Is it easy? No. Is it worthwhile? Absolutely.
This is satisfying and challenging work that makes a real difference in people's lives. We are where you can achieve professional growth with personal fulfillment. We are where you can connect people to making a lifesaving impact. We are where you can partner with individuals, schools, lawmakers, healthcare providers and others to ensure everyone has access to healthier lifestyle choices and proper healthcare. The American Heart Association is where you can make an extraordinary impact.
The Greater Southeast Affiliate of the American Heart Association is recruiting to fill a Youth Market Director (outside sales/fundraising). Under the direction of the Vice President, the Director is responsible for implementing the American Heart Association's Jump Rope for Heart, Hoops for Heart and Red Out for Heart events in 12 Counties (Alachua, Baker, Bradford, Citrus, Columbia, Gilcrist, Levy, Martin, Sumter, Union, Lafayette, Dixie). This position works with school based coordinator, (typically the PE Teacher) to raise funds through our school based educational fundraising program. The Director will act as the American Heart Association liaison with schools in the territory and is responsible for achieving income and recruitment goals through the management of existing customers, and prospecting for and cultivating of new school accounts.
This position requires you to be in the territory 90% of your time and will cover approximately 1 36 schools . We offer a yearly salary, a yearly incentive for exceeding specific fundraising goals for the position, travel reimbursement and a great benefits package which includes: comprehensive health benefits, retirement plan with generous employer contributions, 12 paid holidays per year, paid time off, flexible spending accounts and a Fit-Friendly work environment.
NOTE: This is a work out of your home position that requires daily travel throughout the designated territory (the ideal location for candidate would be Marion/Alachua county.
The ideal person for this position has at least two years' of face to face outside sales or fundraising experience, is self-motivated, organized , works well independently, is comfortable with public speaking and has intermediate to advanced skills in Word, Excel, Outlook, and has worked with a data management program. This person must be able to travel daily to schools in their territory; and, as required, attend meetings and training sessions.
Required Experience
Please review the Qualifications section below to see if you meet the requirements for this position.
Minimum Qualifications
The requirements listed below are representative of the knowledge, skills and ability required. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.
Ability to work independently and resourcefully
Preference will be given to candidates who live in territory.
Proven outside sales experience
Applied knowledge and intermediate skills in windows applications such as Word, Excel and Outlook
Ability to travel within territory 80% of the time and outside the territory 20% of the time for monthly Regional meetings and/or training
Ability to speak publicly
Knowledge of school systems a plus
Some heavy lifting involved
Valid driver's license, proof of at least minimum state required automobile liability insurance and ability and willingness to travel as position demands.
Ability to work outside standard hours as needed, including occasional evenings and weekends.
Satisfactory background checks including consumer credit, motor vehicle, and criminal history.
Education/Experience: Bachelor's Degree and two years of field sales experience or equivalent years of experience in sales, community organization, or fund raising. Supervisory Responsibilities: None
The American Heart Association will not discriminate against any employee or applicant for employment because of age, race, color, religion, sex, gender identity, national origin, disability, veterans' status, sexual orientation, marital status or based on political or religious opinions or affiliations, membership or non-membership in employee organizations.
Job Location
Gainesville, Florida, United States
Position Type
Job Category: Fundraising/Sales
EOE Minorities/Females/Protected Veterans/Disabled


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Property Details
Studio, 0 Bedrooms, 1 Baths, 2 Sleeps
Seven-Night-Minimum! Why should you climb to a roof or walk to the corner to enjoy views? This penthouse unit is actually located on the roof itself, with 360' (including deck) of unobstructed views surrounding the unit. Oversized windows face San Francisco's bay, the Palace of Fine Arts, Golden Gate Bridge, Alcatraz, and the eastern cityscape. Enjoy the tranquility of a beautiful neighborhood, encompassing city living and excellent public transportation - no car required! Ideal for 1-2 adults, this spacious studio is 600 sq' ~ Imagine complete privacy, solitude, and coziness, surrounded by 360' of panoramic views: three sides from inside + one side from the spacious deck. Enjoy coffee and meals on the generous deck, while savoring SF's 'big sky!' ~ The apt has been restored, providing all conveniences of home, including a full kitchen, plus private washer/dryer inside the unit. Every retail convenience is located on the neighborhood's Chestnut Street. Within blocks, walk-jog-run to the Golden Gate Bridge, or relax at Crissy Field's bay and beach. ~ Located half-block from the Palace of Fine Arts, different directions will lead to the Presidio, Fort Mason, Ghirardelli Square, Fisherman's Wharf, and eventually to the Ferry Building's colorful outdoor market. ~ Rent a car for a day-trip, as easy/quick drives across neighboring Golden Gate Bridge will take you to popular bay inlets of Sausalito and Tiburon, or drive 50 minutes to California's beautiful Wine Country. This unit represents everything that is 'best' about San Francisco!
Recent Review
Great Place in the heart of the Marina district
About the Owner
94 Reviews
5 based on 94 traveler reviews
Great Place in the heart of the Marina district
This is a well appointed apartment in a clean, well maintained building in a very safe and walkable area of San Francisco. Rayne provides everything you need for a comfortable stay. There is a bus stop on the corner and you can get anywhere in the city quite easily. San Francisco public transit is very good, no need for a car. Lots of places to eat and drink on Chestnut and Lombard Streets, just a few blocks away. The outdoor patio area has beautiful views and is perfect when the sun shines on a warm day. I would both recommend to others and return myself.
Lovely, Comfortable Studio with a great host!
I recently spent a week at Rayne's lovely studio. I have rented from VRBO and Airbnb several times - this was for sure one of the best experiences. From the initial questions that I had to the booking process and all thru the stay, Rayne was quick in her replies and very professional and helpful. Carolina, the person that manages the apartment, met me upon arrival and gave me a tour of the features, answered questions, etc. There was a lovely welcome basket with various snacks and even included some pears from Carolina's pear tree!
The Studio is located on a lovely street in the Marina District - really nice block with gorgeous houses and landscaping. Half a block away, at the end of the street, is the Palace of Fine Arts which has beautiful grounds to explore. Two blocks away is the Bay, the Marina, and the beaches. The neighborhood is a quiet, residential area that felt very safe to me - I walked a lot both during the day and after dark and always felt safe. 10 minutes away is Chestnut Street which has lots of great restaurants and shopping - try Lucca Deli for authentic italian deli sandwiches and prepared foods. A bit further away, up the hill, are the Cow Hollow and Pacific Heights neighborhoods with more fun shopping and lovely homes.
The apartment was very clean and comfortable. The kitchen is well stocked for anyone that might want to cook. The bed, which is actually a Murphy Bed that pulls down from the wall, was very comfortable. The apartment is on the roof of the building and has 3 large windows - one on each side - that provide panoramic views of the Palace of Fine Arts (which lights up at night), the Golden Gate Bridge, the Bay, and the San Francisco Skyline. Alcatraz is view-able as well. It was very quiet, both during the day and in the evening. Note that there are 4 flights of stairs to be climbed to reach the studio which Rayne notes in the listing - this worked out fine for me(I got my workout for sure! Lol);however, just something to be aware of.
Great stay. Thank you Rayne!
A Perfect Week
What a delightful studio apartment: beautifully furnished, roomy, with views to write home about! The Golden Gate Bridge from one window, the Institute of the Arts from another (take some time to wander here and in the Presidio). We walked everywhere, or took the local bus, dined locally and discovered the entire Marina district. A delight.
And yes, there are four flights to the penthouse, but the journey is worth the view, and you'll come home healthier for the exertion!!
We enjoyed eight wonderful days in San Francisco. This Marina apartment is a little piece of heaven with fantastic views. It was an excellent and convenient base from which to explore the city and Marin County, and provided common living space that a hotel can't offer. There is nothing about the apartment not to love - the location, the view, the furnishings, the extra personal touches by Rayne our wonderful landlady. Thank you so much, Rayne. We look forward to spending another vacation with you again next year. We'll be back for sure!
Totally Amazingl!
We've just returned from a fabulous trip to San Francisco. Aside from San Francisco being such a magical place to visit, we couldn't have stayed at a better spot.
Rayne's apartment is in the perfect location and has the most breath taking views. It literally took my breath away when I walked in the first time. I was also pleased with how open and light and bright the apartment is. There are 3 big windows on 3 sides of the apartment which make you feel you are perched on top of the world.
Rayne has thought of every detail and the apartment is a reflection of this. The cupboards and fridge are stocked with essentials and the furniture and storage arrangements are very efficient. Everything was spotless too!
You are within walking distance of very great restaurants, bars, shops, the beach, and the Golden Gate bridge!! We purchased a 7 day visitor travel pass and found it so easy to get around the city. So much to see and do!! It is also so quiet here, was really ideal. The roof top deck is also a wonderful addition!!
Rayne's qualities as a host are impeccable and the best I have ever encountered. It was a TRUE pleasure dealing with her. I so appreciated all she had done during the reservation process as well. All my questions were always quickly answered and she always went above and beyond providing valuable information.
I was sad to leave and hope to return one day! I would definitely stay here again!! Thanks for everything!!
Beyond Fabulous !!
We've been back home for several weeks now, but can't seem to stop reminiscing over this beautiful apartment. San Francisco is a city we want to keep coming back to, and Rayne's penthouse apartment is definitely where we'll stay. Located in a beautiful neighbourhood, it's extremely quiet, yet only a few blocks away from Chestnut Street, full of restaurants, bars, cafes, shops, etc. There are several grocery stores and delis all within walking distance, and the bus stop is a mere half block away if you want to go further afield. The apartment itself was easy to find, and there was someone on hand to greet us, and walk us through all the features. To echo what others have said, pack lightly and take advantage of the washer & dryer. Besides, 4 sets of stairs is not something you want to tackle with a jumbo sized suitcase. But trust us, the climb is worth it, as the views are absolutely stunning! Many mornings it was hard to tear ourselves away, and it was especially beautiful to sit and enjoy a drink in the evening and watch the city skyline light up. The deck across from the apartment is shared with the rest of the building, but we had it all to ourselves the few times we sat out on it. The apartment was immaculate – absolutely spotless! While it is a studio, we never felt cramped in any way. I was a little worried about how I'd feel after sleeping on a Murphy bed for a week, but it was very comfortable, and we slept really well. The apartment is fully stocked, and had everything we needed. Any questions we had were quickly answered by Rayne. In fact, from our initial booking, to our departure, the communication was fast and clear. You can tell that she takes great pride and care in ensuring her guests have everything they need.
Five stars all the way !! Thanks again Rayne for everything.
Activity and Interests
Seven-Night-Minimum! Nearby, all retail conveniences are located on the neighborhood's Chestnut Street, or walk the other direction to the Golden Gate
Bridge and Crissy Field's bay and beach. Located half-block from the
Palace of Fine Arts, longer walks in different directions will lead
to the Presidio, Fort Mason, Ghirardelli Square, and Fisherman's
Wharf ... and eventually to the Ferry Building's colorful outdoor
market. ~ Easy drives across the Golden Gate Bridge will take you to
the colorful bay inlets of Sausalito and Tiburon, or drive north to
Sonoma and Napa, California's Wine Country.
Rental rates
|Rate Period
|Weekend Night
My Standard Rate
7 night minimum stay
Additional information about rental rates
NOTE: $245./night; 7-night-minimum;
1-2 Adults ONLY
$245./night year-round 7-night-minimum.
Owner's Cancellation Policy
- 12
- Available arrival
- 12
- Available stay (no arrival)
- 12
- Unavailable
Property Type
- studio
- Internet
- non smoking only
- pets not allowed
Location Type
- Ocean View
- Bathroom 1
- Studio, Sleeps 2
- Seven-Night-Minimum! Located on the roof, with large picture windows + deck with panoramic views = 360' Views! ~ 2 blocks to SF bay, walk to Golden Gate Bridge ~ washer/dryer inside Unit ~ full bathroom, including hairdryer, plus additional vanity ~ full kitchen, including dishwasher, microwave, ice-maker, blender, iron, choice of coffee makers ~ free WiFi ~ cable TV ~ CD player ~ radio ~ docking station.


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Krakow City Tour
Discover the beautiful, historic, and culture-packed city of Kraków on a bus and walking tour.
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Qatar Planning Manager Doha Qatar jobs
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Firms with the most Qatar Planning Manager Doha Qatar-related jobs are Profuse HR Consultancy, Al Salama Hospital, Hydrogen Group, Global Connections Manpower, COREcruitement or Prospect HealthRecruitment Agency.
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Neuvoo UAE is the largest job site in the country with over 225072 job vacancies available, 14 of which are Qatar Planning Manager Doha Qatar-related.Show more...
PwC |
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Developing local country transfer pricing documentation. global planning for continuous optimization of... Bahrain, Egypt, Iraq, Jordan, Kuwait, Lebanon, Libya, Palestine, Oman, Qatar, Saudi Arabia and the...
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To manage all HR related functions across 3 entities in the UAE and Qatar. Key role is to provide an... Coordinate career development, succession planning and talent management needs in partnership with line...
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Qatar Airways |
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We have expanded significantly since that time across the UAE, Saudi Arabia and Qatar. We have been... The role is expected to functional lead a team of 3 Contract Aviation Safety, SRLG managers who operate...
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