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So I've been reading a (quite old, I think. It's in the old chess notation anyway.) book called the Art of the Checkmate by Renaud & Kahn. I was beginning to wonder if I'd managed to learn anything from it, when finally I see a chance to play Legal's mate, from the very first chapter!
It's probably old news to a lot of you, but I am just pleased my memory is still working!
Nicely done ... especially to find a chance to play a classical mate like that!
nice game :)
Old news, yes, but if you can pull it off, then you have the right to brag. I studied up on classic traps a year or two ago in the hopes of catching somebody, but it seems that the people I play against already know about them...
10/27/2016 - Four Way Street
by somethin 2 minutes ago
Chess Copyright
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good chess player removed
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Is this cheating?
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How long does it take to be a 2000 rate player?
by vlcosmin 7 minutes ago
Who is the greatest chess player of all time ?? Bobby Fischer ??
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Does Karjakin even have a "puncher's chance"?
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Opening against e4 as well d4?
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text: In windows programs such as access the drop down lists have a prpperty that allows you to add a new item to the list from the list itself, or in other words you can type text into the list box and it auto fills (just like the address bar in your browser). Can this be done with a web form dropdown from asp.net? I would imagine one would need to write a custom control for this, anyone have one or be able to point me in the right direction?

subset: commoncrawl


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Not to worry. Check out the archived thread of the Q&A with Ken Hutcheson, President of U.S. Lawns, and the LawnSite community in the Franchising forum .
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While Palestinians in Gaza welcomed the arrival of basic goods, some were sceptical about its long-term benefits.
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As government casts dragnet for would-be 'terrorists', residents with ties to Syrian rebel groups flee farther north.
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Security crackdown continues on suspected "terror" cells in Beirut hotels following central Beirut hotel bombing.
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An insight into a girls' school in Afghanistan which imposes an even stricter interpretation of Islam than the Taliban.
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text: This is a must visit for me every time I pass through Taos. Never once have I been disappointed. Even dined next to Julia Roberts once. This particular visit I met a friend whom I hadn't seen in many years to rendezvous for lunch. Now this time of year (end of September) I usually go for the front patio, great... More


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If moving your residence seems like a difficult task, then moving a commercial office is something that may give you headaches. Whether it's across town or across the state line, business moves require careful planning.
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02-05-2004, 07:41 PM #1Junior Member
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last week before going away, swole?
ive been following this plan: UNoffical "How to Cut" thread and sample diet... for the last 7 weeks and have seen some good results....I have lost quite alot of fat from my stomach, however i have a little stubborn patch at my belly button area and i cant seem to get rid of it...kinda corny but im heading to the beach (mexico) in 9 days and was wondering if there is any plan i should follow that can get rid of this tiny last but of fat? Would cutting out the carbs completely work? should i increase morning cardio intensity? swole i think you once posted a 7 day pre-competition diet. would this be of any help?
02-05-2004, 07:45 PM #2AR Hall of Fame
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- Dec 2002
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- 25,778
If it's a pure fat tissue, actual fat/adipose tissue, then the timeframe you have is not enough time to get rid of it. Low carbs/no carbs/high carbs, still not enough time.
My 7 days to Ultimate Leanness is aimed at a person totally "drying out" after he/she is relatively ripped to the bone already. If one still has fat to lose, then drying out and ridding the body of all Sub-Q water will not hide the fat that is left to be lost.
Got any pics? If so, I can tell you whether it's worth your time or not. I'll be blunt too so you don't waste your time/efforts for an entire week.
Last edited by SwoleCat; 02-05-2004 at 07:50 PM.
02-06-2004, 07:56 AM #3Junior Member
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ill try to get a camera
Last edited by northendninja; 02-06-2004 at 08:04 AM.
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How do I get rid of malware win32/nuquel.E and banker.A
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Re: win32/nuquel.e
Download OTL by OldTimer to your Desktop.
- Close all windows and double click OTL.exe
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- It will produce two logs for you, one will pop up - OTL.txt, the other will be saved on your Desktop - Extras.txt. Post both logs in this thread.
- You may need to use two posts to get it all.
@RealBelahzur - [You must be registered and logged in to see this link.] - Please PM me if I fail to respond within 24hrs.
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Hotel Hispano 2: Find photos, reviews, information, amenities and compare prices for 3 stars hotels in Murcia, Spain.
Hotel Hispano 2 is situated in Murcia and is close to the area's well-known attractions. It is located a short stroll from the Puente de los Peligros and La Merced University.
Hotel description
Hotel Hispano 2 provides comfortable rooms, designed to suit the requirements of any traveller.
Hispano 2 Hotel Murcia is located in the heart of Murcia's entertainment district with restaurants, cafés and nightlife on the doorstep. Cathedral of Murcia is within a few minutes' walk from the hotel.
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Users who viewed Hotel Hispano 2 also viewed these 3 stars Murcia hotels:
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Murcia Resort - Luxury Ground Floors Apartments
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text: My wife and I we're looking for a nice small inexpensive motel in Pismo Beach for a 1 day weekend getaway. After searching for a couple days we came across the Palomar Inn with okay reviews and a great price. We picked this motel for the price and what we thought was a close walk to the Pismo Beach pier.... More


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Target: Barack Obama, President of the United States
Goal: Urge President Obama to retire the penny (one cent piece) to increase economic productivity
The penny is draining millions of dollars from the United States' economy and contributing to higher taxes. According to the US Mint's 2011 annual report the current cost of minting pennies is 2.4 cents per coin. An estimated 5 billion pennies were minted in 2011, costing the US almost $120 million to produce less than $50 million of circulating currency.
Not only is the penny costing the US Mint double its worth to produce, but the National Association of Convenience and Fuel Retailers reports that searching for and handling pennies adds roughly two seconds to every transaction at a cost of some $300 million each year. The time it takes customers and cashiers to sort pennies is inconvenient, wasting businesses' and consumers' time and money. Eliminating pennies will increase economic productivity by cutting the cost of minting and reducing the time burden associated with their use–reasons why Canada eliminated its own one cent piece in 2012.
Greg Mankiw, Chairman of Economics at Harvard University, put it best: "When people start leaving a monetary unit at the cash register for the next customer, the unit is too small to be useful. It is just wasting the people's time–the economy's most valuable resource." Urge President Obama to eliminate the penny in order to increase economic productivity.
Dear President Obama,
It is time to take a progressive step and eliminate the penny from American currency. The one cent piece is costing the nation both in time and money and is not worth keeping in circulation. According to the US Mint's 2011 annual report, the current cost minting pennies is 2.4 cents per coin. In 2011 it cost almost $120 million to produce less than $50 million of circulating currency.
The penny is holding the United States back from economic progress by charging taxpayers for the production of a coin that is economically unsound. Eliminating the one cent piece would increase economic productivity by reducing transaction times for consumers and cashiers, saving them from having to constantly search for, count and hand pennies.
While there are people opposed to eliminating the penny, opposition is largely rooted in self-interest such as lobbying groups backed by the very manufacturers whose zinc is used to produce the coin. Keeping the penny in circulation is a frivolous use of the nation's limited resources. I urge you to consider how burdensome the penny truly is–separate from its historical value–and to retire it once and for all.
[Your Name Here]
Photo credit: Bobby131313 via Wikipedia


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This event is past, other events listed from the same artist:
Andy Live in Orange County, Thanksgiving Concert
Wednesday, Nov 23, 2016
Irvine, CA
Andy & Shani Concert CRUISE Vacation - 3 days PastOct 18-21, 2013 - San Pedro, CA, United States
Aboard the Golden Princess Resort Cruise to Mexico
Bon Voyage Tour and Travel
Contact the organizer for latest event info. Kodoom.com is not responsible for any changes made in the above information. Report or Flag this event
Andy – Andranik Madadian (born April 22, 1958 in Tehran), better known by his stage name, Andy, is an Armenian-Iranian singer and songwriter. Seen by many as an icon and a legend in pop ...


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Christmas Vespers 2016
December 2 2016 - December 4 2016 7:30pm - 9:00pm | Location: Jackson Auditorium
In preparation for the Christmas season, Texas Lutheran University hosts its annual Christmas Vespers service. This celebration of music and word honors the integral role music has played in Texas Lutheran's curriculum and religious and cultural history. Audiences take advantage of the opportunity to hear the ethereal voices of the TLU choirs each year at both the Seguin and Austin Vespers services.
Christmas Vespers 2016: Hope and Quietly Wait
Titled "Hope and Quietly Wait," this year's Vespers service of music and word will feature the TLU Choir, TLU Women's Choir, Kantorei Chamber Choir and the TLU Symphonic Winds and Percussion. Waiting quietly, and with hope for true peace will be the central theme of Christmas Vespers. San Antonio actress, Sam Carter Gilliam, will perform short monologues between the musical numbers which lend context to the musical idea of Advent as a time of quiet reflection.
Christmas Vespers will be held in TLU's Jackson Auditorium at 7:30 p.m. Friday, Dec. 2; 7:30 p.m. Saturday, Dec. 3; and 4 p.m. Sunday, Dec. 4. Tickets for each Vespers service are free but required.
If you are interested in attending the Vespers service in Austin at St. Martin's Evangelical Lutheran Church, it will take place on Sunday, Dec. 11, at 4 p.m. This Vespers service is free and no tickets are required.
Service Schedule
Friday, Dec. 2, 2016 at 7:30 p.m. in Jackson Auditorium
Saturday, Dec. 3, 2016 at 7:30 p.m. in Jackson Auditorium
Sunday, Dec. 4, 2016 at 4 p.m. in Jackson Auditorium
Sunday, Dec. 11, 2016 at 4 p.m. at St. Martin's Evangelical Lutheran Church, Austin
Ticket Reservation
Tickets will be available November 1, 2016
All Vespers services at TLU are free and open to the public, but tickets are required due to limited seating.
Tickets are not required for the Vespers service in Austin at St. Martin's Evangelical Lutheran Church on Sunday, December 11, at 4 p.m.
There will be reserved seating for TLU's President's Associates at the Saturday performance. For more information call 830-372-8030 or visit www.tlu.edu/giving/presidents-associates/.
Contact Us
Church Relations Administrative Assistant
Featured Events
Latino Heritage Month Events
October 19, 2016 - November 2, 2016
Family Physics Night 2016
October 27, 2016 6:30 pm - 9:00 pm
Dramatic Media Production: Rhinoceros
November 11, 2016 - November 13, 2016 7:30pm - 9:30pm
Giving Tuesday 2016
November 29, 2016
Christmas Vespers 2016
December 2, 2016 - December 4, 2016 7:30pm - 9:00pm
Winter Baccalaureate & Commencement 2016
December 17, 2016
Cokie Roberts, Brown Distinguished Lecture
March 29, 2017 7:30 pm - 9:00 pm
Dramatic Media Production: One Man, Two Guvnors
April 7, 2017 - April 9, 2017 7:30pm - 9:30pm


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- Song Title
- Movie Name
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- Lyricist
- Year
- Decads
- Music Director
- Cast
Rahee Hu Albela Mai Dil Kaa Nahee Maila Lyrics from Chhaila Babu sung by Mukesh. This song is composed by Laxmikant Pyarelal & Written by Hasrat Jaipuri. Chhaila Babu movie stars Naaz, Subhiraj, Jeb Rahman, Ranjana Kadam. Chhaila Babu Releases on Jan 01, 1967.
Lyrics : Rahee Hu Albela Mai Dil Kaa Nahee Maila
Movie : Chhaila Babu
Music Director : Laxmikant Pyarelal
Singers : Mukesh
Lyricists : Hasrat Jaipuri
Genre : N/A
rahee hu albela mai dil kaa nahee maila yaro kehte hain log mujhe chhaila chhaila babu kehte hain log mujhe chhaila rup kee shehjadee hai yaro meree laila yaro kehte hain........ sair sapate karta mai jaa pahuncha phir khet me chand see ujali ladkee baithee thee thandee ret me are dekh key meree surat pehle toh gussa ley aayee chal jo meree dekhi kuchh sharamai kuchh muskayee kehne lagee woh mai teree mere sajan chhaila rahee hu albela........ pyar kee dhun pe maine jab chhedee apnee raginee sun key mera gana phir chham se aayee kaminee are hans key hamko dekha ik jalva phenka dur se dil kee bat ko maine phir kah dala uss hur se mil hee gai mastanee meree dilruba chhaila rup kee shehjadee hai..........
|O Ho Ho Mai Babu Chhaila Chhaila
|Kishore Kumar
|Chhaila Tera Chhaila Mann Ko Bada Sataye
|Lata Mangeshkar
|Tere Pyar Ne Mujhe Ghum Diya
|Mohammad Rafi
|Kyon Jhukee Jhukee Hain Palke
|Lata Mangeshkar, Mohammad Rafi
|Movie Name
|Release Date
|October 27, 2016
|Ae Dil Hai Mushkil
|October 28, 2016
|October 28, 2016
|2016 The End
|November 04, 2016
|30 Minutes
|November 11, 2016
|View more upcoming movies
|Movie Name
|Released On
|1:13:7 - Ek Tera Saath
|October 21, 2016
|31st October
|October 21, 2016
|October 21, 2016
|My Father Iqbal
|October 21, 2016
|Train To Busan
|October 21, 2016
|View more latest movies


text:
Whoever said the afterlife would be easy...
Noël Coward is the toast of twenties society...a brilliant playwright, composer and entertainer whose witty songs and pithy lines don't quite tell the full story. Goldenhurst, his house in Kent, provides a place where he can be himself. And more importantly where he can be with his lover, Jack, without fear of arrest or judgement...
But their romantic idyll is ruined, first by the arrival of Noel's demanding relatives and then by a mysterious death...
Many years later, film star and Coward fan Richard Stent buys the house from its current owner. But Richard has problems of his own. Goldenhurst is supposed to be a respite from all his worries but this is a house with a very rich and not always pleasant history. And more than one thing is about to go bump in the night...
A haunting tale of love, obsession and a house full of spirits, not all of them blithe...
"synopsis" may belong to another edition of this title.
Briefs Encountered is quite as hilarious, clever, stylish, charming and wickedly witty as Noel Coward... It would easier to stab a puppy or slap a baby than to dislike such an utterly lovable book.' ( Stephen Fry 2011-12-08)
Julian Clary is a smart and as funny as ever...a delicious airy treat (Elizabeth Buchan Sunday Times)
So clever, so funny, so romantic, and scary too. I don't know how she does it! ( Fay Weldon)
So funny ... Clary switches effortlessly between eras and demonstrates an inspired eye for comic detail. ( Daily Mail 2012-03-30)
Clary has produced a surprisingly complex, artfully constructed and mostly very convincing novel, which isn't bogged down by its intelligence or his obvious research. Indeed, it's all dressed up in delightfully accessible, sharp prose that's full of gags. ( Daily Mirror 2012-03-30)
Noel Coward meets Julian Clary in this dark and wickedly funny ghost story!
"About this title" may belong to another edition of this title.
£ 2.75
Within United Kingdom
Book Description Ebury Press (Fiction), 2012. Hardcover. Book Condition: Brand New. 384 pages. 9.45x6.38x1.34 inches. In Stock. Bookseller Inventory # zk009193883X
More Information About This Seller | Ask Bookseller a Question
Book Description Ebury, 2012. Hardcover. Book Condition: New. book. Bookseller Inventory # 009193883X
More Information About This Seller | Ask Bookseller a Question


text:
The definitive reference guide to using AutoCAD's complex 3D capabilities. AutoCAD veteran George Head offers users a clear, thorough examination of each 3D feature, providing instructive examples and practical applications of each. A concise, comprehensive introduction provides helpful information on using the book, plus hardware and software requirements for working in 3D.BAT file 559 Accuracy, importance of 167 Advanced Modeling Extension book on 231 holes in 3D surfaces with 352 memory required ... use coordinates 4-13 viewing model 13-15 terminology 16-19 AutoCAD EXTRAS Manual 362 AutoLISP creating and loading programs ... 417-19 Base point 12 Base view 146- 47 Beams, in house model 461-73 Bit, defined 321 Bit-map materials 315-16 Blocking basicanbsp;...
|The AutoCAD 3D companion
|George O. Head
|Ventana Communications Group - 1993-01-01


text:
Home | Submit a file | New | Categories | RSS Feeds | Blog
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"MacX Free Apple TV Video Converter" snapshot
MacX Free Apple TV Video Converter is a 100% free video to Apple TV converter software which enables you to free convert most popular videos like AVCHD, MKV, M2TS, AVI, MPEG, WMV, FLV etc to Apple TV compatible format like MP4 MP3 format on Mac OS.
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Asoftech Photo Recovery is an easy to use data recovery software available to restore your photos, music and videos from local PC and removable devices. No matter multimedia files have been deleted accidently, or memory card has been formatted.
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Mac OS, Other
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22.89 Mb
Date added:
August 12, 2014
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Description from the Publisher
MacX Free Apple TV Video Converter is a 100% free video to Apple TV converter software which enables you to free convert most popular videos like AVCHD, MKV, M2TS, AVI, MPEG, WMV, FLV etc to Apple TV compatible format like MP4 format on Mac OS. In addition, it owns the ability to extract the audio from source video to save as MP3 music. Users are also able to take favorite picture from the video in JPG or BMP file.
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Jooyeon from the Popular K-pop girl group After School is the new model for Marco Underwear, and recently filmed a sexy TV commercial to air on Fuji TV.
Jooyeon took a short break from the promotion of After School's new ablum, and flew to Japan to film the video, showing off her runway walk in lingerie.
The commercial sends a strong message, 'What makes women beautiful is not the men but underwear'.
The staff at the filming studio were totally drawn into her powerful and beautiful presence, reports K-Pop Insider.
View pictures of other celebrities who are popular for their fitness and glamour.


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|Go to Replacement product
|Vicon Industries
|Model Code
|Telemetry Type
|Physical Specifications
|Dimensions mm: 254 x 79 x 203
|Model Info
|The V1 101C System 10 Desk-Top multifunction control is designed to control autopan and tilt drives, lens functions, and housing functions. It also controls wipers , washers , and camera power functions.
|UGAP facilitates access to Tetrapol, Tetra and satellite products from Airbus Defence and Space
Public Safety users in France can now purchase Airbus Defence and Space radio equipment with a single click, from UGAP...
|Proxim launches QB-10150-LKL - long-range version of Tsunami QuickBridge backhaul product
With the new LKL version a 28 dBi high gain integrated antenna allows links in excess of 35 km to be deployed with a low-profile, integrated...
|Siklu EtherHaul-500 V-band radio offers interference-free connectivity on the street
Siklu's EtherHaul-500 provides 200Mbps of real time, interference free connectivity Siklu announces an affordable...


text: "For America to sustain its vitality, promote opportunity, and create a more equitable society during its second 250 years of existence, we must improve the performance of communities and the people who lead and live in them." Sign up for email updates Search for: My TweetsRecent Posts Neighboring Communities and How They Stand Out 7 Oct 2016By San Diego Community Fellow Janice Luna Reynoso Ever since I can remember it has been my pleasure to be a part of two neighboring yet distinct communities. I grew up in living in National ... more Communities of Excellence 2026 Welcomes Rich Umbdenstock to our Board 30 Sep 2016Last month Rich Umbdenstock, former President and Chief Executive Officer of the American Hospital Association (AHA) joined the Communities of Excellence 2026 Board. Mr. Umbdenstock's career includes experience in hospital administration; health system governance, management ... more Communities of Excellence: A Case Study 16 Sep 2016By Kruse Scholar Jasmin Fosheim Sioux Falls, South Dakota is currently experiencing a major shortage of affordable housing units. Struggling families without safe homes to return to each night are forced to wait years before they ... more How do we get more youth of color at the polls? 13 Sep 2016 By San Diego Community Fellow Janice Luna Reynoso Fewer youth of color are participating at the polls than ever. In November of the 2014 general election, only 8.2 percent of eligible voters between the ... more Building Community is a Lifestyle, Not a Job 9 Sep 2016By Kruse Scholar Jesse Nelson All around us, things seem distressed. There a palpable sense of unease in the air, not unlike the feeling one gets when a nasty winter cold is settling in, but ... more ... more posts


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Long Prairie HVAC Contractors
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Recipient rate limiting
Are there any ideas how IronPort could be used to rate limit some internal recipients / domains ? IronPort have excellent tools to throttle senders based on different attributes on different levels but could the same throttling be used for internal recipients ? I would like to find a way to limit certain internal users to be able to receive only certain number of messages per hour.
Time to time such a feature would be useful to protect mailboxes to get overflowed.
I have seen few cases where such a functionality would save a lot of trouble and effort.
1. Botnet bombarding some email accounts creating DoS. (CASE and SBRS will block most but doesn't fully protect)
2. Poor man version of case 1 where chain letter is used for similar purposes. Believe or not but that works, especially in developing countries where users are just learning emailing.
Example of latest chain letter which created a big problems for South Africa First National Bank network:
FNB is giving away 10 entries for this Thursday's "Win aMillion" competition. Just forward this email to four friends and
cc: Emma.nel (a) fnbcorporate.co.za and you will have your 10entries.
Good Luck!
3. Some "developer" user with poor skills have created some badly formulated script and managed to create mailing loop without knowing it.
4. Negligent admin shoots himself in the leg and created mailing loop due bad configuration.
5. Monitoring goes bananas and send hundreds of thousands alerts during weekend
IronPort appliances are very powerful boxes and when they get emails for delivery they sure deliver those, and fast...


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This past Saturday my sister, nephew and sister in law all rode to Alabama. We were bored so we decided to hit up the outlet which were an hour away. While we go there we all decided to get grabbed a snowball since we`e gonna be walking outside in the hot sun. They didn`t have that much options so I got the Green Apple. My sister got Piña Colada, my nephew got Bubble Gum and my sister in law got Strawberry.
Overall, it was okay. He added too much syrups to our snowballs that this is how it looked like when I first got mines. I felt jipped off because I paid more than what I got. Lol! Buck`s snowballs from home are a lot better! Affordable and you get what you pay for. Just saying!
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We have a nice fat pipe for our internet connection but this leads to the majority of our web testing being done on LAN speed environments. We do have a test lab in the with modem connections but does anyone know of a way of simulating modem speed connection from my LAN connected Win 2000 workstation testing with IE 5.5.
Im under the impression that within IIS5 you can throttle the bandwidth delivered to certain IPs therefore you could do this at an IP level to your test lab PCs. If im wrong then performance tool would do this well....
Or simple and cheap add up the size of all the assetts on your page and calculate the download times at say 42k a second (average connection for a 56k modem, most ISPs in the UK anyway)
Client Side: this functionality is included in most web load testing tools including LoadRunner, WAST etc.
If you don't have a dedicated load test tool which offers this and you still want to do it at the client side, you could look into a proxy server on the client side which could limit the speed.
You can also limit this server side with bandwidth throttling e.g. Apache has some bandwidth-throttling modules.
Hope this helps,
You may want to try Cloud, a WAN Emulator. It is available at www.shunra.com.
Keep in mind that if you simulate a 56K modem (at 42Kbs) you still are looking at only one contributing factor. The latencies introduced by the hops over the different networks to reach a webserver can really add up. So be careful to present any response figures to get out of this as actual response times experienced by your users on the internet.
Roland Stens
If you are interested in the experience of your users at thh end of a 58K modem, you will probably want to build a test plan that includes testing across the Internet.
This type of testing will reveal the "real" experience, but interperting the results of your test can be complex.
You will have to run the performance test several times during the normal operating window of your application. (You do know when your peak access time occurs, right?)
You may find that an application that is well behaved in the development lab, or when deployed on a high capacity internal intranet becomes unusable due to network hop delays when deployed externally.
Simply simulating a 56K throttle will not reveal these types of infrastructure problems.
The moral is... when worried about performance, test the entire infrastructure!
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Hire Big Bands in Watsonville, CA
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Teen Held Over Old Firm Disorder
A 16-year-old boy has been arrested in connection with disorder at an Old Firm match.
A £5,000 reward is being offered for information about an ex-prisoner who has been missing since breaching his release conditions.
Police have been trying to trace 33-year-old Johnathan Kelly since he disappeared in September.
They believe he is getting help from other people ''to evade justice'' and said there have been no confirmed sightings of him since he vanished.
Kelly, of Carolside Drive, Drumchapel, Glasgow, had his licence revoked after failing to comply with imposed conditions following his release from Lowmoss Prison on August 21.
Charity Crimestoppers is now offering a reward of up to £5,000 for information leading to the location and arrest of Kelly.
Last month, armed officers responded to a reported sighting of Kelly and sealed off part of Boyd Street in Glasgow's Crosshill area as they searched the area, but he was not found.
Detectives said he is not thought to be a threat to the public but they have warned people not to approach him.
Kelly is described as white, 5ft 9in, with a heavy build and short brown hair.
Earlier this month, police renewed appeals for information on his location and said a ''significant amount of work'' has gone into trying to trace Kelly but there have been no confirmed sightings.
Dave Hunter, regional manager for Crimestoppers, said: "Although Johnathan Kelly is not thought to be a danger to the public, he is considered to pose a risk and it is vital that he is found.
"There must be people out there who know his whereabouts and we urge them to contact us through our anonymous phone line 0800 555 111 or our anonymous online form.
"Crimestoppers does not take any personal details from callers or those who give information through our website. We don't want to know who you are, only what you know.''
A 16-year-old boy has been arrested in connection with disorder at an Old Firm match.
A cheese producer linked to an E.coli outbreak has been told it does not have to destroy stock withdrawn from sale by a food watchdog.
The highest phone box in the UK has been saved from removal following a successful community campaign.
Minimum pricing for alcohol must be brought in as a matter of urgency after judges backed the policy, doctors have said.
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Hotfrog Ireland provides information regarding Corkagh Nurseries in Dublin. Corkagh Nurseries is located at Naas Road, Baldonnell Cross, Dublin Dublin DUBLIN 22 and provides Retail services. Contact them on (01) 459 2336.
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Real Impact Productions: Under Offer
The mockumentary has become a well-explored genre in the worlds of film and television, thanks largely to Ricky Gervais and The Office and films like Spinal Tap and Best in Show but most attempts to harness it for advertising have been knuckle-chewingly excruciating. Now though Real Impact Productions have produced this series of short films for Douglas & Gordon, purporting to be a fly-on-the-wall exposé of an estate agents office (the rather brave premise being that other agents are like this, Douglas & Gordon aren't.) Genuinely funny and nicely textured, it's really impressive, although careful with some of the language…
- Retracing and recreating historic reggae record sleeves with photographer Alex Bartsch
- David Wilson directs deeply moving film B.E.N. about using AI robots to tackle loneliness
- Art and About: Charlotte Trounce celebrates the architectural beauty of museums and galleries
- Riikka Laakso's screenprinted zine is a tribute to Moomin author Tove Jansson
- Sandy Van Helden's illustrations of contemporary culture
- Bompas & Parr explores the strange world of sploshing (NSFW)
- Kodak returns to its 1970s symbol, joining the retrobrand bandwagon
- Kodak unveils the Ektra: its first ever smartphone
- Working Not Working reveals the top 50 companies creatives would kill to work for
- William Knight's socially conscious portfolio of graphic design
- Juan Aballe's photographs of pastoral landscapes filled with wanderlust
- Exclusive first interview with new UK Vice.com editor Jamie Clifton


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The Trenton Board of Public Works held its regular monthly meeting on Tuesday evening at Trenton City Hall and approved the purchase of several items and services for Trenton Municipal Utilities.
TMU Director Chad Davis updated board members on continued efforts by Midland GIS to provide mapping services of TMU's infrastructure. At last month's meeting, Davis said TMU was working the Grundy County Assessor's office on the use of their


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Nails: Cyndi Watson of Umberto Salon in Beverly Hills, Calif., polished a French manicure (left) and sculptured acrylics.
Photography: Claudia Kunin
Hairstyling: Heather Reardon
Makeup: Debi Weeks/Signatures Styling
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Induction of the inducible cyclooxygenase isoform COX-2 is likely to be an important mechanism for increased prostaglandin production in renal inflammation. We examined the effect of lipopolysaccharide (LPS) on regional renal COX-2 expression in the rat. In the inner medulla, LPS injection (4 mg/kg ip) induced a twofold and 2.5-fold increase in the levels of COX-2 mRNA and COX-2 protein, respectively. In contrast, COX-2 expression in the renal cortex was not significantly altered. COX-2 promoter transgenic mice were created using the 2.7-kb flanking region of the rat COX-2 gene. In these animals, LPS injection induced reporter gene expression predominately in the inner medulla. The LPS receptor CD14, usually regarded as a monocyte/macrophage-specific marker, was found to be abundantly expressed in the inner medulla and in dissected inner medullary collecting duct (IMCD) cells, suggesting that it may mediate medullary COX-2 induction. CD14 was present only at low levels in cortex and cortical segments, including glomeruli. In cultured cells, it was abundant in mouse IMCD (mIMCD-K2) cells and renal medullary interstitial cells, but largely undetectable in mesangial cells and M1 cells, a cell line derived from mouse cortical collecting ducts. In the mIMCD-K2 cell line, LPS significantly induced COX-2 mRNA expression, with concomitant induction of CD14. LPS-stimulated COX-2 expression was reduced by the addition of an anti-CD14 monoclonal antibody to the culture medium. These results demonstrate that LPS selectively stimulates COX-2 expression in the renal inner medulla through a CD14-dependent mechanism.
- cyclooxygenase-2
- renal inner medulla
- transgenic mice
cyclooxygenase (COX; prostaglandin-endoperoxide synthase EC 18.104.22.168) catalyzes the oxygenation and peroxidation of arachidonic acid to generate prostaglandin endoperoxides, the immediate precursors of a series of bioactive prostaglandins. Two distinct isozymes of COX have been identified, COX-1 and COX-2 (7, 9, 25, 27). COX-1 is constitutively expressed in a wide variety of tissues and organs, and is generally believed to have "housekeeping functions," especially in stomach, spleen, and kidney. Expression of COX-2, in contrast, can be rapidly induced, most dramatically in inflammatory cells, by proinflammatory stimuli such as cytokines, mitogens, and lipopolysaccharide (LPS). Selective inhibition of COX-2 has been shown to reverse inflammation in various tissues (1). Recently, large-scale clinical trials have demonstrated that COX-2 inhibitors are as potent as nonselective COX inhibitors, such as indomethacin and ibuprofen, in the treatment of various inflammatory diseases, but that they have fewer side effects in stomach and platelets (11, 23, 26). These observations are consistent with the initial speculation that COX-2 is the cytokine-inducible cyclooxygenase, critically involved in inflammatory responses (7, 16).
There is substantial evidence to demonstrate that LPS induces unique inflammatory responses in the kidney. Systemic infusion of LPS causes marked renal vasoconstriction, leading to a marked reduction of renal blood flow, despite a normal or increased cardiac output (2, 18). LPS also suppresses renin release (4, 28) and is associated with an immediate reduction of Na+ and K+ excretion followed by the development of profound natriuresis (19). It appears that local mechanisms underlie LPS-induced alterations in renal function. Prostaglandins are produced abundantly in the kidney, particularly in the inner medulla, and are capable of modulating multiple renal functions, such as renal blood flow (5, 34), renin release (33), and urinary Na+ excretion (3). LPS, infused systemically or administered intrarenally via the renal artery, has been shown to stimulate prostaglandin release from the kidney (29). These observations are consistent with the hypothesis that prostaglandins may be involved in the LPS-induced renal pathophysiology.
The mechanism of action of LPS has been extensively studied. LPS first induces hepatic LPS binding protein (LBP), which functions as a carrier protein in the circulation. The complex of LPS and LBP then recognizes and acts on CD14, which is located on the surface of macrophages (31). CD14 is a 5300–5500 mol wt glycosyl-phosphatidylinositol-anchored membrane protein that is found almost exclusively in monocytes and macrophages and that is often used as a marker of monocyte lineage. Large numbers of studies demonstrate that CD14 binds to LPS and LBP, and mediates LPS-induced cytokine production (6, 37). CD14, therefore, functions as the receptor for the LPS/LBP complex. In support of this notion, transgenic mice expressing human CD14 are hypersensitive to LPS (10).
The current experiments were performed to determine1) whether treatment with LPS affects COX-2 expression in the kidney and whether the induction of COX-2 by LPS differs between different regions of the kidney,2) whether LPS affects COX-2 promoter activity in transgenic mice in vivo,3) whether renal epithelial cells express CD14, and 4) whether CD14 plays a role in LPS-regulated COX-2 expression in renal cells.
We performed experiments in male Sprague-Dawley rats (175–200 g). Rats in the experimental group (n = 16) received an intraperitoneal injection of 4 mg/kgEscherichia coli LPS (Sigma Chemical, St. Louis, MO). Rats in the control group (n = 16) received an intraperitoneal injection of the same volume of PBS. The animals were killed 2, 4, 6, and 8 h after LPS injection and tissue samples from renal cortex and inner medulla were harvested for the determination of COX-2 mRNA and protein. Animals were killed and tissue samples from renal cortex and inner medulla were harvested for the determination of COX-2 mRNA and protein. For RNA extraction, tissue samples were transferred immediately into microcentrifuge tubes containing 300 μl of TRI Reagent (Molecular Research Center, Cincinnati, OH) and snap frozen in liquid nitrogen. Samples from the other kidney were collected for protein analysis.
Cell culture experiments.
The source of cultured cells, including mouse inner medullary collecting duct cells (mIMCD-K2), mouse cortical collecting duct cells (M1 cells), mesangial cells, and renal medullary interstitial cells (RMIC), has been described previously (39). To study the effect of LPS on COX-2 expression, we serum deprived confluent mIMCD-K2 cells for 12 h and then treated them with LPS (100 ng/ml) for various periods of time. To study the effect of CD14 receptor blockade on LPS-induced COX-2 expression, we serum deprived confluent mIMCD-K2 cells for 12 h and treated them with rmC5–3 (0.05 mg/ml; PharMingen, San Diego, CA), an antibody against recombinant mouse CD14, before stimulation with LPS.
cDNA synthesis and PCR.
Details of the methods used have been published previously (39). The sequences of the oligonucleotide primers and their location in the published cDNA sequences were as follows:1) COX-2: sense 5′-ACA CTC TAT CAC TGG CAT CC-3′ (bp 1229–1248) and antisense 5′-GAA GGG ACA CCC TTT CAC AT-3′ (bp 1794–1813) were predicted to amplify a product of 584 bp (9); 2) CD14: sense 5′-GCA GCA GTG GCT AAA GCC TG-3′ (bp 882–901) and antisense 5′-ATC GCC TCT TTG TTT AAG GAA CAC-3′ (bp 1185–1208) were predicted to amplify a product of 627 bp (12). We performed 30 PCR cycles with an annealing temperature of 59°C for both COX-2 and β-actin PCR and 60°C for CD14 PCR. The quantitative assays used 10-fold serial dilutions of cDNA of each experimental sample to ensure that all PCR determinations fell within the linear amplification range. RNA samples subjected to the PCR procedure without prior reverse transcription served as "reverse transcriptase-minus" controls. Water and dissection medium blanks were run to serve as a control for cDNA contamination. We checked genomic DNA contamination by carrying samples through RNA isolation and cDNA synthesis without the addition of reverse transcriptase. Dissection medium and reverse transcriptase-negative samples consistently yielded no product.
Western blotting analysis.
Immunoblotting was performed as previously described (35). Briefly, kidneys were removed and tissue samples from cortex (200 μg) and inner medulla (100 μg) were dissected and homogenized. Tissue homogenates were suspended in loading buffer and subjected to electrophoresis on 10% SDS-PAGE. We transferred the contents of the gels electrophoretically to nitrocellulose membranes and stained them briefly with Ponceau S to determine uniformity of electrophoretic transfer. The membranes were then washed in TBS (20 mmol/l Tris ⋅ HCl, pH 7.5; 150 mmol/l NaCl; and 1% Nonidet P-40), blocked with 5% dry milk in TBS, and incubated with a 1:500 dilution of a COX-2 polyclonal antiserum (Cayman). The membranes were washed three times in TBS, blocked again, and incubated with125I goat anti-rabbit IgG (ICN Biomedicals, Irvine, CA). Finally, we washed the filters three times in TBS, dried them, and quantitated them by phosphorimage analysis using a GS-250 Molecular Imager System and Phosphor Analyst software (Bio-Rad, Hercules, CA).
Experiments in transgenic mice.
We amplified 2.7 kb of previously characterized rat COX-2 promoter sequence from genomic DNA isolated from liver of Wistar-Kyoto rats using a high-fidelity amplification kit (Boehringer Mannheim). The fragment was subsequently subcloned into pnlacf vector upstream of the nuclear localization signal and lacZ gene. The recovery of fertilized eggs, injection, and reimplantation into the oviducts of pseudopregnant female mice were performed with assistance from the Transgenic Core Facility at the University of Michigan (20). We performed genotyping by PCR using the chimeric, construct-specific primers. We obtained F1 mice by breeding the founder mice with CD-1 mice. After two intraperitoneally injected doses of LPS (1 mg/kg each), at 12 and 2 h before death, animals were anesthetized and perfused through the heart with 4% paraformaldehyde. Kidney was removed and cut into pieces. X-gal staining was performed at 30°C for 20 min, with the solution containing 0.2% X-gal, 10 mM sodium phosphate buffer (pH 7.0), 150 mM NaCl, 1 mM MgCl2, 3.3 mM K4Fe(CN)63H2O, and 3.3 mM K3Fe(CN)6.
Effect of LPS on renal COX-2 mRNA and protein expression in vivo.
The effect of a single injection of LPS on the renal expression of COX-2 was determined in tissue samples from inner medulla and cortex. We determined COX-2 mRNA and protein 2, 4, 6, and 8 h after LPS injection by RT-PCR and Western blotting analysis, respectively. Under basal conditions, COX-2 expression was more abundant in inner medulla than in cortex, consistent with previous observations (13, 39). LPS significantly enhanced COX-2 expression at both mRNA and protein levels in the inner medulla, a change that was detectable 2 h after the treatment and lasted over the next three time points. Because the values derived from densitometric analysis of the four time points were not significantly different, the data were pooled. The average increase in COX-2 mRNA and protein in inner medulla after LPS treatment was twofold and 2.5-fold, respectively. In contrast, COX-2 expression in the cortex at either mRNA or protein levels was not significantly altered by LPS. Figure 1 and Fig.2 show representative gels of the effect of LPS on COX-2 mRNA expression in inner medulla and COX-2 protein expression in both cortex and inner medulla, respectively.
Effect of LPS on COX-2 promoter activity in vivo.
COX-2 promoter transgenic mice were created using a 2.7-kb rat COX-2 flanking sequence fused upstream of a nuclear localization signal andlacZ. Genotyping was performed by PCR. Primers were selected to span the junction region between promoter sequence andlacZ gene, thus amplifying a product specific to the chimeric construct (Fig.3 A). The predicted 450-bp products were detected in tail DNA from the transgenic mice, but not in that of CD-1 mice (Fig.3 B). Under basal conditions, β-galactosidase activity of both COX-2 transgenic and wild-type mice, as well as of CD-1 mice, was detectable at low levels in cortex and outer medulla, but not in inner medulla, despite a 12-h incubation. In contrast, LPS treatment induced a marked increase in the β-galactosidase activity in the kidney, predominately in inner medulla of a transgenic founder mouse (Fig.4, A andB). In the example shown in Fig. 4, the intense staining was detected within 15 min after the incubation with X-gal. A similar pattern of staining was also observed in three other LPS-treated F1 mice from a separate founder mouse (Fig.4 C), although intensity of staining varied.
Distribution of CD14 and LBP mRNAs in kidney regions.
RT-PCR for CD14, LBP, and β-actin was performed on 1 μg of total RNA from cortex and inner medulla of adult Sprague-Dawley rats (Fig.5). The identity of the products was confirmed by restriction digest (data not shown). CD14 mRNA was abundantly present in inner medulla, whereas the signal was barely detectable in cortex. LBP mRNA was present in both regions, with a modestly higher abundance in inner medulla than in cortex.
Localization of CD14 in microdissected nephron segments.
We determined CD14 mRNA expression in microdissected nephron segments using RT-PCR. An abundant amount of CD14 mRNA was consistently detected in inner medullary collecting ducts (IMCD; Fig.6). Low levels of expression were also regularly detected in glomerulus. Products were not consistently observed in proximal convoluted tubule, cortical collecting ducts, cortical thick ascending limb, or arcuate artery.
mRNA expression in cultured cells of kidney origin.
mRNA expression of CD14 was assayed in cultured cells of renal origin, including two types of cortical cells (mesangial cells and M1 cells) and two types of medullary cells (mIMCD-K2 and RMIC). Total RNA from confluent cells was isolated and was subjected to RT-PCR. The CD14 signal (from undiluted cDNA) was abundantly present in mIMCD-K2 and RMIC, the two medullary cells, but the signal was only detected at an ∼100-fold lower abundance in mesangial and M1 cells, the two cortical cell lines (Fig. 7), consistent with the regional distribution pattern of CD14 shown in Fig. 5.
Effect of LPS on COX-2 and CD14 mRNA expression in cultured mIMCD-K2 cells.
Confluent mIMCD-K2 cells were serum deprived for 12 h and then treated with LPS (100 ng/ml) for 4 and 16 h. PCR for CD14 and COX-2 was performed on 1:10 and 1:100 diluted cDNA, ,and PCR for β-actin was performed on 1:100 and 1:1,000 diluted cDNA. Treatment of LPS (100 ng/ml) significantly stimulated both CD14 and COX-2 mRNA expression in a time-dependent manner. Stimulation of CD14 peaked at 4 h and declined at 16 h (Fig. 8), whereas stimulation of COX-2 occurred at 4 h and peaked at 16 h after the treatment (Fig. 8). To test the role of CD14 in mediation of LPS-stimulated COX-2 expression, we used CD14 antibody to neutralize CD14. As shown in Fig.9, addition of the CD14 monoclonal antibody significantly reduced LPS-stimulated COX-2 mRNA expression in cultured mIMCD-K2 cells.
In contrast to the constitutive presence of COX-1 in many organs and tissues, COX-2 is a cyclooxygenase isozyme that is characterized by absent or low expression under resting conditions and whose production is dependent on stimulation by cytokines, mitogens, and a variety of tissue-specific activators (7). However, COX-2 has been found in the kidney in the absence of specific interventions that typically stimulate its expression. The vast majority of renal constitutive COX-2 production in rats and humans is found in the medulla, both in RMIC and collecting duct cells (39). In addition, a highly localized expression of COX-2 in macula densa cells and/or surrounding thick ascending limb cells is also present in the renal cortex (39). Commensurate with the character of COX-2 as a highly inducible enzyme, renal expression of COX-2 mRNA, as well as COX-2 protein, was found to be regulated by a number of interventions, including changes in dietary salt intake and ureteral obstruction (30, 39). The current study was designed to determine the effects of in vivo and in vitro LPS treatment on renal COX-2 expression because LPS treatment has been shown to be a potent stimulator of de novo expression of COX-2 in cells that do not express it under resting conditions (17, 22).
Our data show that LPS caused a sustained upregulation of COX-2 mRNA and protein in the renal medulla, but not in the cortex. The inner medullary cells affected by LPS include IMCD cells, based on the present RT-PCR determinations in cultured mIMCD-K2 cells. The time course of LPS-stimulated COX-2 expression is similar to that reported earlier for induction of the enzyme by LPS in macrophages (38). Upregulation of COX-2 activity is consistent with several reports showing increased renal prostaglandin production after LPS treatment (19). In one previous study, LPS administered in a comparable dose to conscious rats did not affect the expression of COX-2 mRNA in kidney tissue (36). The present results would suggest that the stimulatory effect of LPS on medullary COX-2 may have been compensated by an opposite effect in the cortex, resulting in an absent net effect for the whole kidney.
The differential response is also seen in COX-2 promoter transgenic mice. The transgenic mice were created using a 2.7-kb rat COX-2 flanking sequence fused upstream of nuclear localization signal andlacZ. Under basal conditions, there was barely detectable β-galactosidase activity in the kidney of the transgenic mice, not consistent with the constitutive COX-2 expression in the kidney of wild-type mice. It is possible that the regulatory elements responsible for the constitutive expression of COX-2 in the kidney are located outside the 2.7-kb flanking region of the COX-2 gene, that the transgene is not integrated correctly into the chromosome, or that the staining method is not sensitive enough to detect the low level of constitutive reporter activity. We found that these animals exhibited high responsiveness to LPS treatment by expressing the reporter gene predominately in the renal medulla, consistent with the LPS-induced COX-2 expression pattern in the kidney, suggesting that the 2.7-kb flanking region contains LPS-responsive elements. We observed a certain degree of variability in intensity of reporter activity between different founder lines that may be due to differences in integration sites in chromosomes.
The mechanisms responsible for the differential effect of LPS on COX-2 expression are unclear. However, expression of a number of genes, including COX-2 and bNOS, has been shown to be differentially regulated in the two regions; for example, salt restriction stimulates the expression of bNOS and COX-2 in the cortex, whereas salt loading stimulates their expression in renal medulla. Thus it is not unprecedented that cortical and medullary COX-2 are subject to differential regulation by LPS. However, the observation of renal expression of CD14 in the present study may provide a clue to understanding the mechanism for the differential regulation of renal COX-2 by LPS. CD14 is a glycosyl-phosphatidylinositol-anchored membrane protein that is regarded as a marker of monocyte/macrophage lineage. The expression of CD14 has been reported to be restricted to monocytes, neutrophils, and macrophages. Thus our finding of constitutive expression of CD14 in the kidney was unexpected. Consistent with predominant CD14 expression in the renal medulla, we found that CD14 expression was much more abundant in mIMCD-K2 cells and RMIC compared with cells derived from cortex, such as mesangial cells and M1 cells. These findings suggest that CD14-expressing medullary cells may behave like macrophages, being capable of responding to inflammatory stimuli with the induction of COX-2 and possibly of cytokines. In support of this idea, we demonstrated that, in the mIMCD-K2 cell line, LPS treatment significantly induced COX-2 mRNA expression with concomitant induction of CD14 expression. Furthermore, we significantly reduced the LPS-stimulated COX-2 expression by adding a CD14 monoclonal antibody to the culture medium. Taken together, this demonstrates the presence of both constitutive and inducible expression of CD14 in the renal medulla that is, at least in part, responsible for the site-specific induction of COX-2 in renal medulla by LPS.
Involvement of a medullary NO system may be another reason for the different response of COX-2 to LPS in cortex and medulla. The renal medulla is known to be a rich source of NO production (40), in agreement with the abundant expression of nitric oxide synthases (NOS), including the cytokine-stimulatable or inducible form of NOS, iNOS or NOS II (24). Stimulation of iNOS production by LPS may therefore be more pronounced in the medulla than in the cortex. Because NO has been shown, in a number of preparations, to stimulate COX activity (8, 32), it is conceivable that the medullary localization of COX-2 activation by LPS reflects the local interaction with inner medullary iNOS.
Mesangial cells are known to play a role in the pathogenesis of various types of glomerular nephritis. In support of this notion, mesangial cells in vitro are capable of responding to different inflammatory interleukins by producing prostaglandins. Studies by Guan et al. (14,15) have shown that interleukin-1β induces COX-2 expression in cultured mesangial cells through a MAPK-mediated pathway. The low expression of CD14 in mesangial cells shown in the present study suggests that COX-2 stimulation during inflammation in these cells may primarily reflect an indirect response to cytokines such as interleukins rather than a direct response to LPS. It was an unexpected finding, however, that LPS did not detectably increase mRNA and protein levels of COX-2 in the renal cortex, suggesting a complex mechanism for the in vivo regulation of COX-2. It is possible that COX-2 in mesangial cells in vivo is stimulated by LPS through an indirect mechanism. The stimulation of COX-2 in mesangial cells may be compensated by inhibition of expression in tubular segments. In preliminary observations, we found that COX-2 expression in thick ascending limb was downregulated by LPS (data not shown). Future studies need to examine the detailed mechanism for the differential response at the cellular level.
The kidney is an organ that serves excretory and endocrine functions, but it has been recognized as an organ important in immune responsiveness as well. In certain aspects, differentiated kidney cells resemble immune cells with a potential to produce different cytokines in response to proinflammatory stimuli. For example, isolated renal interstitial cells in culture have been shown to produce cytokines, whereas skin fibroblast cells in the same condition did not (21). Clinical studies have shown that renal failure is frequently accompanied by severe infections that, in many instances, cannot be reversed by dialysis. Loss of renal immune function is likely to contribute to the infections seen in renal failure. On the other hand, disturbance of the renal immune system is known to be linked to various types of acute or chronic nephritis. Demonstration of CD14 in renal medulla and its role in LPS-stimulated medullary COX-2 expression is strong evidence in support of an important role of the kidney, particularly of the renal medulla, in immune response.
In summary, these studies report differential regulation of COX-2 expression in renal cortex and inner medulla by LPS. LPS stimulates COX-2 expression only in the renal inner medulla and not in the cortex. CD14, the LPS receptor, was found constitutively and selectively expressed in the inner medulla and in cultured cells derived from renal medulla. In the mIMCD-K2 cell line, LPS significantly induced COX-2 mRNA expression, with concomitant induction of CD14 and the LPS-stimulated COX-2 expression was reduced by the addition of CD14 monoclonal antibody to the culture medium. These results demonstrate that LPS selectively stimulates COX-2 expression in renal inner medulla through a CD14-dependent mechanism.
We gratefully acknowledge Bruce Stanton for providing mIMCD-K2 cells and Richard Palmiter for providing pnlacf plasmid.
Address for reprint requests and other correspondence: J. Schnermann, NIDDK, NIH, Bldg. 10, Rm. 4DA51, 10 Center Dr., Bethesda, MD 20892-1370 (E-mail: jurgens{at}intra.niddk.nih.gov).
This work was supported by National Institute of Diabetes and Digestive and Kidney Diseases Grants DK-37448, DK-39255, and DK-40042. Support was provided, in part, by the General Clinical Research Center (GCRC) at the University of Michigan, funded by a grant (M01RR00042) from the National Center for Research Resources, National Institutes of Health, US Public Health Service.
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
- Copyright © 1999 the American Physiological Society


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Pupils listen to a teacher reading of The Little Red Hen, identify the story elements and sequence them. They talk about different jobs associated with providing food and act out a play based on the story. Be aware that although several Common Core standards are listed by the publisher, they do not give specifics about how each portion of the lesson might meet them. You will need to think through this if you are working on the Common Core.
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AEG 61040K Owner's Manual
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To protect the surface and maintain the clean and bright finish, use a silicone conditioner. This will Protect the surface whilst cooking. It is very important to clean the surface soon after every use, when the glass is still warm. Do not use metallic sponges, powder abrasives or corrosive sprays. Depending on the dirt level we recommend: - Slight stains: use a moist clean rag. - Burnt or soiling may be removed with a special razor scraper ( fig. 5 ); be aware that the razor can cause wounds. - Liquid marks, caused by boil-overs, can be removed using vinegar or lemon. - Pay attention not to let spilled sugar burn onto the element. In this case turn the switch off and clean the surface with hot water and the razor blade scraper. - After a period of time metal reflex and scratches (fig. 6) may appear due to the wrong cleaning and the wrong use of the pots. The scratches cannot be removed, but they do not compromise the good operating of the hob. - Don�t use steam jets for the equipment cleaning.
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- For accurate prices please enter the date of your trip!
- Provision can be requested on the spot
Our best offer
90 € / person / night, without provision
Description of the accommodation:
Rooms, services Eurostars de la Reconquista Oviedo is offering 142 tastefully furnished rooms to those seeking accommodation in Oviedo. Since the hotel has an own restaurant, you may also try fine local meals on the spot. Parking possibility for your vehicle is available nearby. The bar provides a nice atmosphere for a romantic evening talk or a personal meeting. Your valuables may be the safely secured in the safe. Wireless Internet connection (WiFi) is available at the hotel, enabling portable devices and smartphones to go online. The exact scope of services offered by Eurostars de la Reconquista Oviedo (including those for an extra fee) is available on the information sheet below.
Payment Advance payment by credit card or providing credit card guarantee may be necessary for finalizing the reservation, depending on the chosen date, room and price.
Arrival, opening hours The earliest arrival time is 14:00, the latest time to leave the rooms is 12:00. You will be able to speak with the personnel in Spanish and English languages.
Eurostars de la Reconquista Oviedo foglalás, szoba árak Foglalás, szoba árak
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Accommodation services
Parking (23 €/night)
WiFi in shared spaces
- Finnish sauna
- Hairdresser's parlour
Bar, Elevator, Safe deposit box
Own restaurant
Hairdryer, Desk in the room, Mini-bar


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Train work well on track
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Work to prepare for the electrification of Auckland's rail network is on target with the installation of overhead wires along the eastern line expected to be completed by mid July.
Just over 500km of wire is being installed by KiwiRail along the network from Swanson in the west to Papakura in the south.
On the eastern line only the Mt Wellington to Westfield Junction section still needs to be put in place, Auckland Electrification Project director Nicholas French says.
The next step will be to go through a process to test the lines before they are signed off as operational. With the installation of the live wires, there are important safety messages to consider, Mr French says.
Height restrictions have been introduced at the 31 level crossings on Auckland roads within the electrified area.
At most level crossings, the height restriction will be 5 metres, but 11 will have a reduced height restriction of 4.25m.
Roadside signs will warn people of the risks of getting too close and display the height restriction at that level crossing. There are also anti-climbing guards and screens in place to deter people from coming into contact with wires.
The first passenger services using the electric trains will start on the Onehunga to Britomart line on April 28 and will be up and running on the eastern line in the latter part of the year.
Go to kiwirail.co.nz and click on Auckland Rail Electrification for information.
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If anyone would like to come along to a Zumba class, I go to Zumba with KheShan at Seaford Primary School.
It's on Mondays and Wednesdays at 6:30pm and is $10 per class (I think your first one is less and there a multi-class discount passes that you can buy).
It's a really fun, friendly class and the instructor is awesome!
There's a really good mix of fitness levels and ages and the instructor makes everyone feel welcome and comfortable.
I'd love to meet some of you there :)


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Rush Limbaugh, one of America's biggest names in talk radio has apparently signed a 200 million pounds – eight year deal – with Premiere Radio Networks.
The news has leaked from an upcoming interview with The New York Times, which is expected to be published on Sunday. He currently hosts a daily three hour show on over 600 radio stations across the USA. More than 14 million people listen to him at least once a week.
The deal works out around $400m and will keep him on-air till 2016 at the Clear Channel Radio-owned network.
Hear more radio industry news like this at the top of the hour on inRadio - the radio industry's very own radio station. Listen now in a new window whilst you continue reading RadioToday or press play below.


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Dati bibliografici
Titolo: Random House Parent and Child Puzzles, ...
Casa editrice: Random House Puzzles & Games
Data di pubblicazione: 1995
Condizione libro: very good
Gently used. Expect delivery in 2-3 weeks. Codice inventario libreria 9780812925432-3
Riassunto: Learning and fun go hand-in-hand in this unique series. The side-by-side design lets grown-ups and kids work simultaneously on similar crosswords, word finds, and cryptograms, sparking plenty of interaction and diversion.
Recensione: This is a clever collection of 44 pairs of puzzles designed for parent and child to solve together. Each puzzle can be worked separately--one is written at an adult's level and the other a child's level--but the two come together to form a larger puzzle. Perhaps it's a riddle and answer, or two sets of words that must be combined to form a third set. It's a great way to spend time with your kids and introduce them to the entertaining and mind-sharpening world of word games.
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Share house
Share house
4 Bedrooms
4 Beds
1 Bathrooms
1 Baths
4 Flatmates
4 Flatmates
|Room Type
|Private room
|Shared bathroom
|Flexible with furnishings
|Oct 24, 2016
|Length of Stay
|2 weeks to 2 months
|Weekly rent
|2 weeks rent
|Included in rent
|Available but not included in rent
|On street parking
I have 3 rooms available for rent in Artarmon. It is located 3-5 minutes walk to Artarmon Train Station and local shops where you have access to different eateries, cafes & restaurants, bakeshops, convenience stores like 7-11, Australia Post, newsagent, dry cleaning, 24/7 fitness, chemist, Artarmon Medical Center, fruit shops, butcher and many more...
From Artarmon train station to following train stations:
St. Leonards - 3 mins.
North Sydney - 8 mins.
Wynyard - 15 minutes
Town Hall - 20 minutes
Chatswood - 3 mins.
Macquarie Park - 15 minutes
If you want to catch a bus, it is 7 - 10 minutes walk to the bus stop.
If you want to walk to Chatswood, it is only 10 minutes walk to Chatswood CBD where you have access to Coles, Woolworths, Chemist Warehouse, Westfield, Myers, Target, Kmart, Aldi and many many more to list...
If you have a car, parking is on the street. The neighborhood is quiet and safe.
I only accept females. I would prefer only one female each room but if you want to take one friend/relative with you to share the rent, I will only allow 2 females for each room @ $150 each. Otherwise, take the whole room at $275.
Rooms are furnished but if you want it unfurnished, we can do so if the furnishings are not set up yet. Furnishings that I will put for each room are: Single bed, Desk and chair, Wardrobe, Fan
Furnishings in the lounge is a three seater sofa and TV
Furnishings in the kitchen is dining table, toaster, kettle, microwave, washing machine, fridge, cutleries, pots and pans.
Basic requirements:
*Females only
*Work with Children Check (WWC) and National Police Certificate as I work in childcare - I am happy to reimburse you.
*2 weeks deposit
*2 weeks rent paid in advance
The house will be available on 22 October 2016 but if you need it ASAP, please let me know.
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|The Thai Artarmon
|~ 4 min walk
|Maiya Indian Curry House
|~ 4 min walk
|Hu Wah Cafe
|~ 4 min walk
|Bakers Delight Artarmon
|~ 4 min walk
|Artarmon Gourmet Chicken
|~ 5 min walk
|Salvage Coffee
|~ 5 min walk
|Dels Pizzeria
|~ 5 min walk
|Everyday Canteen
|~ 5 min walk
|Little Thai Place
|~ 5 min walk
|The Wilkes
|~ 5 min walk
|Sushi-Ya Artarmon
|~ 5 min walk
|Rahmen Genki
|~ 5 min walk
|Artarmon Station, Hampden St
|~ 4 min walk
|Hampden Rd Before Francis Rd
|~ 5 min walk
|Pacific Hwy S Of Mowbray Rd
|~ 5 min walk
|Pacific Hwy Before Mowbray Rd
|~ 5 min walk
|Pacific Hwy After Mowbray Rd
|~ 5 min walk
|Pacific Hwy S Of Mowbray Rd West
|~ 5 min walk
|Mowbray Rd Near Pacific Hwy
|~ 6 min walk
|Pacific Hwy Opp Howarth Rd
|~ 7 min walk
|Pacific Hwy Near Gordon Av
|~ 8 min walk
|Pacific Hwy Near Sutherland Rd
|~ 8 min walk
|Pacific Hwy Near Howarth Rd
|~ 8 min walk
|Anegawa Enterprises Pty Ltd
|~ 5 min walk
|Lucky Mart
|~ 5 min walk
|Mays Mart
|~ 7 min walk
|~ 11 min walk
|Indian Grocery House
|~ 14 min walk
|miracle supermarkets
|~ 15 min walk
|ALDI Chatswood
|~ 16 min walk
|Woolworths Chatswood Rail
|~ 16 min walk
|~ 16 min walk
|Coles Supermarkets
|~ 16 min walk
|Artarmon Station
|~ 6 min walk
|~ 17 min walk


text:
Does rune spawn randomly regardless of player in the map OR player must stay in the map for certain time?
Does rune spawn randomly regardless of player in the map OR player must stay in the map for certain time?
they have a cooldown for spawning (not positive the length, feels like 10 mins or so), and they will not spawn if there is no player in the map
They generally spawn after you kill enough mobs and their cooldown is 10 minutes or so?
The Rune respawn time is about 15 minutes, which is the same amount of time for the Rune usage cooldown. They're only respawned when you kill monsters (number seems to vary all the time).
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Force Sub Categories To Use Parent Category Templates
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When doing WordPress theme development the first thing you will learn about is the template hierarchy, this is the order of templates WordPress will use to display the content. WordPress actually only needs one template file to work correctly, the index.php file. Depending on what content you are viewing you can create a different page template to show this in a different way. For example if you are looking at a post content then WordPress has a hierarchy of template files . . . This content is restricted to subscribers
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