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qualifiedcodersNEW DELHI, India
Our team of highly qualified professionals helps to deliver end to end solutions from project initiation, planning, design, development, QA, deployment, support and maintenance. Throughout the project execution we also provide documentation services (SRS, Wireframes) for all artifacts related to each phase of SDLC.Apart from this we also provide high quality website design services and web promotion services for any type of business. Range of Services ►Web, Mobile and Desktop Custom/Bespoke Application Development ►IT And Business Consulting ►UI/UX & Designing ►Quality Assurance and Testing ►Marketing & Branding ►IT Infrastructure Services ►Writing & Translation Services ►Data Entry Services Our Technology Stack: .Net,ASP.NET,C#,PHP, MSSQL, Mysql, Azure, Android, iPhone,HTML5, CSS3, Sitefinity, Kentico, Umbraco, KnockoutJS, NodeJS, AngularJS, EmberJS, Javascript, Kendo UI, jquery, Dotnetnuke, MEAN, Lightswitch, Silverlight,WPF, WCF, Web Services,MVC, Wordpress, Bootstrap,Java
ProfSoftStudioBalti, Moldova, Republic of
Good day! I am an Unix engineer with great experience in Unix, Virtualization of many types, Networking, Security, Monitoring, Backup and many other subjects. Thanks!
IndexpageAHMEDABAD, India
We are a software development company focused on web and mobile application development. Our Skills Mobile Application Development: GPS Tracking Apps Developer: Gps & Google Map, Location Based Apps, On Demand Services Apps, Stock & Financial Apps E-Commerce Apps: Magento Mobile App, Prestashop Apps, Custom eCommerce Mobile App Multimedia Apps: Photo/camera/Video Streaming /Audio - Photo Sharing/Uploading/Downloading/Filtering/Editing/Mixing/Recording/Processing Medical: Health And Fitness Online Chatting/Messaging/ Utility App, 360 Virtual Reality, Augmented Reality, Oculus App Hire Developer iOS - iPhone App, iPad Apps, Android Developer PHP/MySQL - CakePHP, Zend, Laravel, Yii, CodeIgniter, Symphony Developer ASP.NET - C#, VB.NET Developer, SharePoint JavaScript - AngularJS Developer, jQuery, AJAX Open Source Developer - WordPress, Drupal, Joomla, Magento, Prestashop, OpenCart, e-Commerce Developer Hire Web Developer/Mobile Developer/Software Developer/Application Developer
infowayNorthridge, United States
We are high class professional designers, expert developers and flawless QA Testers in Theme and Template designing; CMS like Wordpress, Joomla, Drupal; Ecommerce like Magento, Opencart, Woocommerce, Virtuemart; on MVC 5/6; PHP on CI, Yii, Symphony, Zend, Laravel frameworks; Apps in Android and IOS platforms. Quick facts at a glance: - Year Founded: 2004 - Number of Employees: 110 - ISO 27001 and BBB certified - Offices in Northridge,LA and India It's always a pleasure working with Infoway as it provides: 100% productivity Long-term Relationship 100% Growth Friendly Knowledgeable and available staffs Best value Convenience A fast finish Quality Service NO HIDDEN COSTS A Trusted Partner for life
best1Pune, India
We are best1, our name and our client's testimonials say all about our work. We have been on Freelancer for over 10 years and have successfully delivered over 500 projects. We are one of the top and most recommended freelancer. We have a team of 100+ working together to provide best in class solutions to our esteemed clients. We follow a matured process for all our projects and ensure that each project gets due importance. Our sole aim here is to deliver successful projects and to ensure that our clients are happy and satisfied. We have good infrastructure, licensed software, 8 MBPS leased line Internet, Fortinet Firewall and IBM X3650 M4 Server to support our work. We follow ISO 9001:2008 and ISO 27001:2013 certified processes to maintain and deliver quality projects. We sign NDA for the project we work on and keep upmost confidentiality and security of our client's data. We work on various open source technologies and across various verticals. Working with us, rest assured you will be delighted and will pride your choice. If you need any further information, please feel free to contact us. Look forward to work with you on your next project.
mobispec23London, United Kingdom
We are based in London, UK. Please review our portfolio: http://polydiaminfosoft.com Mobile apps: www.mobispector.com Skype: MobiSpector London Contact Us: http://mobispector.com/contact-us/ Some of our recent work: http://www.soccercoachpro.com/ http://higginsmarketing.com/ http://paramountwd.com/ http://mobispector.com/ http://hypu.org.uk/ http://www.oqps.ie/ http://dentovations.com/ http://www.herbalist.com/ http://ellistaylor.com/ MobiSpector/PolydiamInfosoft Limited was founded in 2008 after realising how much potential there was in the mobile app development/web development industry. Just over four years later, Mobispector's award-winning team of planners, project managers, developers, designers and testers have delivered over 60 consumer and enterprise mobile apps for a diverse range of clients. I would suggest we set up a call to discuss the requirements in detail before we can submit a fixed cost proposal, so please get in touch with us for further discussion. Thanks MobiSpector Team Tel: 02084931060
phpMaestroJaipur, India
Logicspice is serving for more than 9 Years all across the globe in the Industry of Software Development. We have been awarded as one of the Top 10 Leading Solution providers over Freelancer.com. We specialize in bespoke or Tailor made Web and mobile Applications with experts available on various technologies including but not limited to iPhone, Android, Magento, Wordpress, Joomla, Drupal, CakePHP, Zend, Yii, PHP/MySQL, ASP.Net etc. We have clients from around the globe, including Australia, Canada, USA and UK. Please check our Portfolio here and also check Client's feedback for the projects completed by our team. Our Service Highlights- ---------------------------------- - Custom and Complex Web application and Mobile Apps Development using custom coding (cakePHP, CodeIgniter, Yii, Zend) or open source solutions (Magento, Drupal, Joomla, Wordpress). - Experience with popular solutions like CRM, ERP, CMS - Digital Marketing including SEO and SEM - Experts in PHP5, HTML 5, CSS3, jQuery, Ajax, Java Script, XML, Objective C, Java Additional Benefits- --------------------------- - Extensive quality check and testing to ensure the quality of work delivered. - Documenting the requirements with innovative ideas, to ensure everything remains well planned from initial stage - Believe in providing required suggestions for improvements of the planned application - Support and Maintenance with good availability and quick turnaround - For Designing projects, we provide UNLIMITED REVISIONS until the client is completely satisfied - FREE technical support for 30 business days after the completion of the project and thereafter at a nominal cost of USD 15/hr. Team- ----------- - iPhone - iPad and Android Apps Developers - Web Designers - PHP Programmers - HTML Developers - Specialized open source Developers - Team Leaders - Project Managers - Quality Assurance engineers - SEO Experts - Business Analysts CONTACT US TODAY FOR YOUR PROJECT! Click the "Hire Me!" Button on my profile.
influxiveLondon, India
Erixir offers end to end web solutions ranging from re-furbishing, revamping and customizing of existing websites to development of new websites from the scratch. With an experience and expertise for more than 9 years in web development and working in most assorted business verticals, Erixir has gained enough proficiency to securely guarantee the development and delivery with Our Protection Policy - 100% Guarantee / Safety of your Money Erixir provides top-notch, professional website design & development services. Our highly skilled web designers and web developers provide complete front-end and back-end development based on the latest technologies and trends. Erixir supports companies to establish complete mobile access to their website and software applications. We deliver native mobile applications impeccably connected to an online service, corporate business application or informational online resource.
omtechnologiesDelhi, India
Om Technologies was established in 2001. With around a decade of experience in designing logos, developing websites, web applications, web portals, Om Technologies is now a brand in itself. We have always been innovative and adaptive in nature. Starting from static HTML sites in 2001, we always adapted latest technologies to meet the client requirements and make our clients stay ahead of the world. We have been developing websites, web portals, ecommerce solutions in PHP, MySQL, CSS, HTML, XHTML, DHTML. As with advent of web 2.0, we adapted the AJAX technologies to develop the most intereative user interfaces Our SEO experts are real gurus in optimizing client websites for obtaining top ranking in search engines. They do best Search Engine Marketing for the selected keywords and have extensive experience in building backlinks. Our clients either give us web designing / web development /web application works on contract basis or they hire our PHP / ASP C# VB .NET / MySQL experts on hourly or monthly basis thus getting their work done as per their requirements We have expertise in Open Source Code developement and customizations. We have developed various modules, components and plugins for OSCommerce (OSC), vTiger, PhpBB, Word Press (WP), Typo3, Os Date, Dolphin Community, Cube Cart, Joomla, Facebook, Joomres etc.
maplesoftwaresNew Delhi, India
Experts in Java, JEE, Spring, Struts, JPA, PHP, Code Igniter, JQuery, HTML5, CSS3, AngularJS, KnockOutJs, BootstrapJs, Crossrider


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Taming of the Press, The: Cohen v. Cowles Media CompanyABC-CLIO 1999; US$ 96.00
Cohen v. Cowles Media Company changed the course of First Amendment media law. After a quarter century of decisions interpreting the First Amendment to give media organizations preferential treatment, the Supreme Court ruled in 1991 that the Constitution did not give the press immunity from the laws ordinary citizens must obey. The American Bar... more...
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Lifestyle media � books, magazines, websites, radio and television shows that focus on topics such as cookery, gardening, travel and home improvement � have witnessed an explosion in recent years. Ordinary Lifestyles explores how popular media texts bring ideas about taste and fashion to consumers, helping audiences to fashion their lifestyles as well... more...
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We have all heard that Americans are increasingly turning to blogs for news, information, and entertainment. But what is the content of blogs? Who writes them? What is the consequence of the population's growing dependence on blogs for political information? What are the effects of blogging? Do readers trust blogs as credible sources of information?.... more...
Tabloid BritainTaylor & Francis 2005; US$ 41.95
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Key Themes in Interpersonal CommunicationMcGraw-Hill Education 2007; US$ 38.00
What are the main influences on the formation of self-identity? What role do language and non-verbal communication play in the construction and display of identity? This work explores the socio-cultural surrounding in which interpersonal communication takes place, and considers the interface between interpersonal and mass communication. more...

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ELECTROLUX ETN1282V 1KOK Owner's Manual
Product Reviews
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Reviews of our other manuals
Radom?r Hason - 2009-01-05
Complete service manual and easy to read,Lots of good info in this one
kayron kayron - 2009-07-01
Vladimir Pupkin - 2009-10-24
Manual received - Many thanks for an excellent service.
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Thanks for the high Quality rez scans. this was exacly what I needed to repair my old boombox .. ;o)
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This is the only place I found this manual. Manual is complete and well worth the price. Thanks.
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SAN FRANCISCO — San Francisco Supervisor Sean Elsbernd is leading a fight to abolish the system that virtually guarantees Muni operators $3,000 each in bonus pay, as part of an arrangement that ties their compensation to the two highest-paying transit systems in the country. For the full story, click here.
The full low-floor, three-door, two-staircase double-deck bus is being shown at the LTA-UITP Singapore International Transport Congress and Exhibition in Suntec City.
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By James Strudwick Both Arsenal and Everton are preparing to fight for the signature of Hull City teenager Josh Tymon after his impressive display against Bristol City in the League Cup, The Mirror ar... [Read More]
The Premier League will soon be upon us again this weekend as the title race and the battle for survival begin to really take shape. Nowhere else in the country will that be more prominent than at the... [Read More]
Another weekend of Premier League football is almost upon us as Arsenal travel to the Stadium of Light to face Sunderland. The two side's fortunes could not have been more opposite this season. ... [Read More]
According to Spanish media outlet Don Balon (via The Daily Express,) Arsenal are interested in signing Sergio Aguero from Manchester City. The Argentine has been at City since 2010 in which time he ha... [Read More]
According to Italian outlet CalcioMercarto (via The Daily Express,) Arsenal attempted to sign AC Milan youngster Manuel Locatelli but could not get a deal to go through over the summer. Arsenal report... [Read More]
Despite only picking up a point in their last league game against Middlesbrough, Arsenal go into their showdown with Sunderland this weekend as red-hot favourites. Arsene Wenger's men sit joint ... [Read More]
By Jack Malone Arsenal are said to be closely watching Middlesbrough teenager Harry Chapman as they look to strengthen their development squad, according to a report in The Mirror. The 18-year-old is ... [Read More]
Alex Iwobi has enjoyed a tremendous rise into the Gunners' first-team in the last year or so, with the highlight being a continued number of starts in Arsene Wenger's side this season. The... [Read More]
Arsenal booked their place in the quarter-finals of the EFL Cup with a 2-0 victory over Reading on Tuesday evening but it seems like this trophy will continue to elude Arsene Wenger, who has not lifte... [Read More]
When it comes to football, I like to think of every conceivable way in which Arsenal can gain an advantage, against every current and future opponent. Which is why I always look out for upcoming oppos... [Read More]


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As your employees increasingly use a mix of applications from the office or the road, measuring and quantifying end user experience has become nearly impossible. Endpoint Agents track performance and user experience of browser-based internal and external apps, including SaaS applications such as Salesforce and Office 365. You can even monitor virtual desktops in your environment. Sign up for a free trial to see Endpoint Agents in action.Sponsored Listing
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GIS Software
Modular, touch screen friendly navigation system with GPS tracking, realtime routing engine and support for various vector map formats. Works on a wide range of devices, from computers to smartphones.131 weekly downloads
a free and open map of wireless communicating objects34 weekly downloads
OpenDMTP is a protocol/framework that allows communications with small devices (mobile phones, PDA's, and other high-latency/low-bandwidth devices), especially geared towards the transmission of GPS, temperature, and other remote monitoring information.63 weekly downloads
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Gosmore is an OpenStreetMap navigation application. It can display the map in 2D and 3D. It also provides searching and turn-by-turn routing capabilities. All the data is stored offline in it's own binary format.81 weekly downloads
Java-based SAS.Planet minimalist mobile client3 weekly downloads
Add Internet Communications, Security, and Messaging to your Apps. Components for every major protocol from FTP to IMAP to SNMP, SSL and SSH security, S/MIME encryption, Digital Certificates, Credit Card Processing, ZIP compression, Instant Messaging, Shipping and Tracking, and e-business (EDI) transactions. Download Now!Sponsored Listing
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OziExplorer Utilities
OziUtils is a collection of small utilities for OziExplorer (http://oziexplorer.com). Currently, there's one which provides "Index map" feature work-alike for OziCE.2 weekly downloads
GaiaPocket is a mobile GIS client written on .NET Compact Framework 2.0, using common OGC protocols such as WMS and WFS. You can use GaiaPocket libraries to build your own GIS client in no time.1 weekly downloads
SourceForge Local Government
This project exists as a central meeting place for those wishing to share in-house developed applications and tools for local government (and related industries) in Australia.1 weekly downloads
GPSMobile is a java library providing access to GPS devices from mobile devices like PDAs or mobile phones. It uses J2ME Personal Profile (JSR 062) specification. The core of the library uses GPSTool package from Dinopolis. A demo application is provided.1 weekly downloads
Geospatial SDK for Windows Mobile. It supports geo-datas such as shapefile and mapinfo, to fetch information and render them. It also supports GPS tracking. It makes developing embedded GIS application very easy.1 weekly downloads
GPS tracking software for PocketPC which uses bitmap images and rotates the image as you move. Load multiply images as needed based on the present position. Can load tracks and POI-s from mps files.1 weekly downloads
Enebro is a system for capture and maintenance of graphical and alphanumeric information on site. Capture all types of geometrics elements and their attributes in a simple form or in more complex structures.
GDL - GPS Data Logger
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GK Navigation
GKNavigation offers mobile phone-Users with -o2 Germany- (operator-dependent) the ability to locate their position (accuracy in average 10-200 Meters) by their mobile and this Application for PocketPCs or smartphones free of charge,without a GPS reciever
GPS Mario
Mobile GPS location publisher / trip tracker by SMS, HTTP, email etc.
GPS Reading Tools
Small GPS reader for bluetooth GPS's for PalmTop's Made for Windows Mobile 2003 Version 2
HikeTracker GPS
Hiketracker GPS ist ein Projekt für die Navigation im Gelände mittels PocketPC und GPS-Empfänger mit Bluetooth-Verbindung in Java. Hiketracker GPS is a Java based project to navigate in the landscape on PocketPC and GPS-receiver with Bluetooth-connectio
Nazooke! lets people track their friends, vehicles, animals, or other things. It sends the user's position in defined intervals to a remote server which will show the user's position on a map.
Open Mobile GPS
The goal of this project is to realize an open source complete graphical GPS environment(not just a tracker but also a navigator with vocal instructions) compatible with all the device that are j2Me compliant and with all the NMEA GPS receiver even BT.
A C# GPS application for Windows Mobile, with map data from OpenStreetMap.org and waypoint management mainly aimed at supporting data from GeoCaching.com.


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Regulatory Compliance Specialist jobs
Neuvoo UAE provides 125 regulatory compliance specialist job opportunities in UAE. Submit your application now and get the best position available.
Regulatory Compliance Specialist vacancies in UAE
The regulatory compliance specialist is a profession in the Facilities category. In total on Neuvoo UAE, more than 125 job opportunities are indexed from dozens of sources such as company career sites, job boards, placement agencies and newspapers. Similar job vacancies from this sector such as Regulatory Affairs, Regulatory Analyst, Regulatory Management, Regulatory Specialist, Scout or Supervisor Cleaning are also very present.
Firms hiring regulatory compliance specialist candidates
Executive Solutions Employment Agency, Crescent Careers, Convate, Client of iQuest Consultants Pvt. Ltd., Visage or Lals Group are among the top firms looking for applicants like this.
Employment statistics
Each month on average, 63 new job vacancies are indexed on Neuvoo UAE. Emirates where these candidates are most in demand are Dubai or Abu Dhabi.
Neuvoo UAE: the largest job search website in UAE
Neuvoo UAE lists over 231634 job opportunities in UAE, 5,908 of which are in facilities and 125 of which are regulatory compliance specialist job opportunities.Show more...
Michael Page |
Abu Dhabi, Abu Dhabi
- 18h ago
Arabic speaking compliance specilist required. Looking for somebody with 2 5 years experience to join a... A combination of exposure to both the UAE and a top tier regulatory jurisdiction. An understanding of...
Michael Page |
Abu Dhabi, Abu Dhabi
- 1d ago
Excellent opportunity working one of the world's wealthiest institutions. Looking for a specialist in... Reports directly to Senior Manager. Regulatory Compliance, though will also take direction from the Head...
Naukrigulf |
Abu Dhabi, Abu Dhabi
- 1d ago
Job Description. support the compliance team in the implementation of the compliance framework. support... Bull A Combination Of Exposure To Both The Uae And A Top Tier Regulatory Jurisdiction. &Bull An...
OS2i |
Dubai, Dubai
- 12h ago
Create and maintain accurate records for regulatory reporting as required by law and by Western Union... Participate on project teams representing AML Compliance. Assist in compliance related calling programs...
Jobzed |
Al Ain, Abu Dhabi
- 9d ago
Global, branded MNC requires an Arabic speaking Scientific & Regulatory Affairs Specialist to join there... Ensuring that all ingredient and formulas are in full compliance with the relevant laws and regulations...
Jobzed |
Dubai, Dubai
- 9d ago
Global, branded MNC requires an Arabic speaking Scientific & Regulatory Affairs Specialist to join there... Ensuring that all ingredient and formulas are in full compliance with the relevant laws and regulations...
McDermott |
Dubai, Dubai
- 12h ago
Support the planning and execution of the internal HSE audit and compliance assurance programs. Provide... Liaising with statutory and regulatory bodies for renewal of licenses, obtaining clearances, consents...
Energy_Jobline |
(Unspecified city)
- 1d ago
Our Storm Water Compliance Specialist careers provide you with an opportunity to be responsible for... Knowledge of water quality regulations, and experience working in regulatory compliance. experience...
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Description: Parish Church of Saint Mary
Grade: II
Date Listed: 10 November 1951
English Heritage Building ID: 210680
OS Grid Reference: SD8043310863
OS Grid Coordinates: 380433, 410863
Latitude/Longitude: 53.5940, -2.2971
Location: N Back Rock, Bury BL9 0LD
Locality: Bury
County: Bury
Country: England
Postcode: BL9 0LD
Incorrect location/postcode? Submit a correction!
Parish Church of St
10.11.51 Mary
Of medieval origin, but the neatly detached west broach spire is of 1844-5 and the
rest by Crowther, of 1871-6. Late C13 style. Coursed rubble. Long nave, aisles,
clerestory, chancel with polygonal apse, north road - Turret with fleche, baptistery
with polygonal apse projecting to north at west end. High interior with 4 wide arches
to nave, tall spindly metal screen, stained glass in apse and south chapel probably
by Hardman and monument to Lieutenants Robert and George Hood (d 1821 and 1823).
Listing NGR: SD8043310863
This text is a legacy record and has not been updated since the building was originally listed. Details of the building may have changed in the intervening time. You should not rely on this listing as an accurate description of the building.
Source: English Heritage
Listed building text is © Crown Copyright. Reproduced under licence.


text:
At any given time we will all come to the stage where getting data they usual way is not effiecient enough. In VB6 Using ADO you could use similar code to the following to run through a parameter array:

 For x = 0 To UBound(vParamArray)
 adoCommand.Parameters(x + 1).Value = vParamArray(x)
 Next x

 Set strStoredProc = adoCommand.Execute

Which would return / Update / Add / Delete our data, all we would need to do is make sure that we follow the correct order of parameters in the SQL SP. In .NET however I have found that this method doesn't work anymore, not without exact param Names (Although they now don't need to be in the correct order)!

Is there a way to run a Stored Procedure, adding the Parameter values as the code above in .NET without having to do all the extra work?


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|24 Reviews
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When you book through the HomeAway checkout, your booking is backed by our Book with Confidence Guarantee™ offering comprehensive payment protection.Learn more
Artistic Charming Guest House on Ten acres. One BDR, Queen Size Bed. Living Room with twin Daybed and Open Kitchen. Enjoy staying only four minutes from beautiful Avila Beach or seven min from downtown San Luis Obispo, CA. Perfect for couple who wants serenity in See Canyon. Only 1.7 Miles in Canyon
surrounded by Large Oak Trees and Year round Creek
on property. Enjoy Wine Tasting just down the road at Kelly's Winery. So sorry, but no pets.
Lonnie and Glenn were perfect guests. It was such a pleasure to have them here.
They were so easy to talk with and we felt liked we had known them for years. It is such a delight when guests appreciate the magical beauty here in See Canyon. If you are wondering rather to have Lonnie as a guest she was as "splendid" as you will get. I hope they will come back again, we would love to have them!
This is a super cozy place-great hostess, very welcoming, lots to do in the area. I would definitely return!
My husband and I enjoyed an amazingly peaceful and relaxing three days at the beautiful guest cottage. The house was everything described and more. Nicole met us on arrival and showed us around the private property where we were also greeted by a doe and her two fawns. The house was clean and very cozy with plenty of towels and amenities in the bath as well as the kitchen. The bed was so comfy, we slept late each morning. We enjoyed coffee in the mornings and wine in the evenings on the patio covered by the canopy of a large oak tree. I recommend this property to anyone looking to get away and yet be so close to Avila Beach, San Luis Obispo and anywhere along the central coast. Thank you, Nicole and Walt, for sharing your little bit of heaven on earth!
For anyone looking to get away from it all, this is a great choice. Set among Apple orchards between Avila Beach and SLO, and away from the 101, it is quiet and secluded. Nicole is a gracious host and goes out of her way to make sure everything is right. We saw deer every morning. Highly recommended.
We were very impressed by the property. The owners have put a lot of attention into the details of decorating and making this guest house comfortable. It was exactly as advertised!! The owner, Nicole, met us and showed us where everything was that we might need. She promptly answered my questions. I would highly recommend anyone looking for a lovely retreat to stay here.
We only got to stay for 1 night but the property is amazing and we would love to stay again if we are in the area. We saw a few feet grazing in the morning just outside our window. Owner is very pleasant and welcoming. Must stay if you are in the area.
My daughter and I had such a special time at your little cottage. It was so beautiful and peaceful. I love that your are close to everything in town but feel that you are a thousand miles away. One morning we woke up and had the pleasure of seeing a very pregnant deer feeding in the meadow. The beds were comfortable, the cottage was very clean and really a work of art. We appreciated all the helpful hints of where to go for our Mother's Day massages and places to dine. Nikki, thank you for everything and we will be back next Mother's Day! Marcia and Clacey
|Rate Period
|Weekend Night
My Standard Rate
1 night minimum stay


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Master's Theses (Plan A and Professional Engineering Design Projects)
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Soy protein hydrolysate; solubility, thermal stability, bioactivity, and sensory acceptability in a tea beverage.
Lee, Jookyeong (2011)
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Soy protein hydrolysate; solubility, thermal stability, bioactivity, and sensory acceptability in a tea beverage.
Lee, Jookyeong
Issue Date
Thesis or Dissertation
Protein solubility affects the quality of many food products, especially high-protein beverages (>4.2% protein). Beverages formulated with soy protein at >4.2% protein content are currently unavailable in the market due to thermal challenges affecting the protein solubility and also due to flavor challenges. Enzyme hydrolysis of soy protein may lead to enhanced solubility and thermal stability. However, bitter taste caused by hydrolyzing soy protein is a limiting factor to using SPH in food applications. Therefore, controlled and limited hydrolysis of 2-8% is required to minimize the production of bitter peptides. Another advantage of controlled hydrolysis of soy protein is the release of bioactive peptides. Among the many physiological benefits of bioactive peptides, antihypertensive activity has gained much attention. Therefore the objectives of this work were 1) to optimize hydrolysis conditions of soy protein for enhanced solubility, thermal stability and bioactivity, while maintaining low degree of hydrolysis (DH); and 2) to determine the sensory acceptability of lemon flavored iced tea beverages formulated with soy protein hydrolysate at various concentrations. Soy protein isolate (SPI) was hydrolyzed by papain, bromelain, trypsin, and alcalase at various enzyme levels while keeping other hydrolysis conditions constant. Enzymatic activities were optimized based on DH measurements. Degree of hydrolysis (DH) was measured using O-phthaldialdehyde method. Samples were subjected to SDS-PAGE analysis to monitor enzyme selectivity. ACE inhibitory activity was measured using a standardized assay. For determination of solubility and thermal stability, solutions (5% w/v) of hydrolysates and SPI were subjected to heat treatment at 95°C for 60 min followed by centrifugation and determination of protein content in the supernatant using a nitrogen analyzer. Solubility and thermal stability at different protein concentrations (1%, 2.5%, 5%, and 7%) in a black tea beverage were also measured. Alcalase-hydrolyzed SPI was chosen for the sensory study as it had the highest solubility, thermal stability, and antihypertensive activity among the hydrolysates. Nine formulations were prepared, including a control which was a tea with no added protein and the four different protein concentrations containing either SPI or alcalase-hydrolyzed SPI. One-hundred-one subjects rated overall liking, flavor, aroma, appearance, and mouthfeel on an 11-point hedonic scale and rated intensity of bitterness and off flavor on a line scale. Hydrolysates produced using 2.654 GDU of bromelain, 0.012 AU-A of alcalase, 19,680 USP units of papain, and 235,000 U of trypsin had DH of 3.98%, 3.57%, 6.77%, and 2.60%, respectively, thus were selected for further experiments. Use of different enzymes resulted in distinctive differences in hydrolysis patterns. Among the four produced hydrolysates, alcalase-hydrolyzed SPI was highly soluble (75.25%) at relatively high protein concentrations, thermally stable, and possessed the most pronounced antihypertensive activity (IC50=0.263 mg protein/ mL). Lemon flavored iced tea beverages fortified with alcalase-hydrolyzed SPI at various protein concentrations (1 up to 7 % protein) were found to be acceptable. The beverages formulated with the hydrolysate at 1%, 2.5%, and 5% protein content were liked as much as those without added protein. Beverages formulated with up to 5% hydrolysate also had significantly less bitter taste and off flavors than SPI beverages. These findings can be utilized in the development of high protein fortified beverages, which is acceptable with relatively low bitter taste and noted physiological benefits.
Food science
Appears in collections
Master's Theses (Plan A and Professional Engineering Design Projects)
University of Minnesota M.S. thesis. August 2011. Major: Food science. Advisors: Baraem Ismail and Zata M.Vickers. 1 computer file (PDF); xiii, 117 pages, appendices A-L.
Suggested Citation
Lee, Jookyeong
Soy protein hydrolysate; solubility, thermal stability, bioactivity, and sensory acceptability in a tea beverage..
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