text:
Most Cited Gene Articles
The most cited articles published since 2011, extracted from Scopus.
Update on Wnt signaling in bone cell biology and bone diseaseDavid G. Monroe | Meghan E. McGee-Lawrence | Merry Jo Oursler | Jennifer J. Westendorf
Volume 492, Issue 1, January 2012, Pages 1-18
For more than a decade, Wnt signaling pathways have been the focus of intense research activity in bone biology laboratories because of their importance in skeletal development, bone mass maintenance, and therapeutic potential for regenerative medicine. It is evident that even subtle alterations in the intensity, amplitude, location, and duration of Wnt signaling pathways affects skeletal development, as well as bone remodeling, regeneration, and repair during a lifespan. Here we review recent advances and discrepancies in how Wnt/Lrp5 signaling regulates osteoblasts and osteocytes, introduce new players in Wnt signaling pathways that have important roles in bone development, discuss emerging areas such as the role of Wnt signaling in osteoclastogenesis, and summarize progress made in translating basic studies to clinical therapeutics and diagnostics centered around inhibiting Wnt pathway antagonists, such as sclerostin, Dkk1 and Sfrp1. Emphasis is placed on the plethora of genetic studies in mouse models and genome wide association studies that reveal the requirement for and crucial roles of Wnt pathway components during skeletal development and disease. © 2011 Elsevier B.V.
The Ccr4-Not complexMartine A. Collart | Olesya O. Panasenko
Volume 492, Issue 1, January 2012, Pages 42-53
The Ccr4-Not complex is a unique, essential and conserved multi-subunit complex that acts at the level of many different cellular functions to regulate gene expression. Two enzymatic activities, namely ubiquitination and deadenylation, are provided by different subunits of the complex. However, studies over the last decade have demonstrated a tantalizing multi-functionality of this complex that extends well beyond its identified enzymatic activities. Most of our initial knowledge about the Ccr4-Not complex stemmed from studies in yeast, but an increasing number of reports on this complex in other species are emerging. In this review we will discuss the structure and composition of the complex, and describe the different cellular functions with which the Ccr4-Not complex has been connected in different organisms. Finally, based upon our current state of knowledge, we will propose a model to explain how one complex can provide such multi-functionality. This model suggests that the Ccr4-Not complex might function as a "chaperone platform". © 2011 Elsevier B.V.
Function of alternative splicingOlga Kelemen | Paolo Convertini | Zhaiyi Zhang | Yuan Wen | Manli Shen | Marina Falaleeva | Stefan Stamm
Volume 514, Issue 1, January 2013, Pages 1-30
Almost all polymerase II transcripts undergo alternative pre-mRNA splicing. Here, we review the functions of alternative splicing events that have been experimentally determined. The overall function of alternative splicing is to increase the diversity of mRNAs expressed from the genome. Alternative splicing changes proteins encoded by mRNAs, which has profound functional effects. Experimental analysis of these protein isoforms showed that alternative splicing regulates binding between proteins, between proteins and nucleic acids as well as between proteins and membranes. Alternative splicing regulates the localization of proteins, their enzymatic properties and their interaction with ligands. In most cases, changes caused by individual splicing isoforms are small. However, cells typically coordinate numerous changes in 'splicing programs', which can have strong effects on cell proliferation, cell survival and properties of the nervous system. Due to its widespread usage and molecular versatility, alternative splicing emerges as a central element in gene regulation that interferes with almost every biological function analyzed. © 2012 Elsevier B.V.
MAP kinase signalling cascades and transcriptional regulationShen Hsi Yang | Andrew D. Sharrocks | Alan J. Whitmarsh
Volume 513, Issue 1, January 2013, Pages 1-13
The MAP kinase (MAPK) signalling pathways play fundamental roles in a wide range of cellular processes and are often deregulated in disease states. One major mode of action for these pathways is in controlling gene expression, in particular through regulating transcription. In this review, we discuss recent significant advances in this area. In particular we focus on the mechanisms by which MAPKs are targeted to the nucleus and chromatin, and once there, how they impact on chromatin structure and subsequent gene regulation. We also discuss how systems biology approaches have contributed to our understanding of MAPK signaling networks, and also how the MAPK pathways intersect with other regulatory pathways in the nucleus. Finally, we summarise progress in studying the physiological functions of key MAPK transcriptional targets. © 2012 Elsevier B.V.
Regulation of the human catalytic subunit of telomerase (hTERT)Michael Daniel | Gregory W. Peek | Trygve O. Tollefsbol
Volume 498, Issue 2, May 2012, Pages 135-146
Over the past decade, there has been much interest in the regulation of telomerase, the enzyme responsible for maintaining the integrity of chromosomal ends, and its crucial role in cellular immortalization, tumorigenesis, and the progression of cancer. Telomerase activity is characterized by the expression of the telomerase reverse transcriptase (TERT) gene, suggesting that TERT serves as the major limiting agent for telomerase activity. Recent discoveries have led to characterization of various interactants that aid in the regulation of human TERT (hTERT), including numerous transcription factors; further supporting the pivotal role that transcription plays in both the expression and repression of telomerase. Several studies have suggested that epigenetic modulation of the hTERT core promoter region may provide an additional level of regulation. Although these studies have provided essential information on the regulation of hTERT, there has been ambiguity of the role of methylation within the core promoter region and the subsequent binding of various activating and repressive agents. As a result, we found it necessary to consolidate and summarize these recent developments and elucidate these discrepancies. In this review, we focus on the co-regulation of hTERT via transcriptional regulation, the presence or absence of various activators and repressors, as well as the epigenetic pathways of DNA methylation and histone modifications. © 2012 Elsevier B.V.
History of gene therapyThomas Wirth | Nigel Parker | Seppo Ylä-Herttuala
Volume 525, Issue 2, August 2013, Pages 162-169
Two decades after the initial gene therapy trials and more than 1700 approved clinical trials worldwide we not only have gained much new information and knowledge regarding gene therapy in general, but also learned to understand the concern that has persisted in society. Despite the setbacks gene therapy has faced, success stories have increasingly emerged. Examples for these are the positive recommendation for a gene therapy product (Glybera) by the EMA for approval in the European Union and the positive trials for the treatment of ADA deficiency, SCID-X1 and adrenoleukodystrophy. Nevertheless, our knowledge continues to grow and during the course of time more safety data has become available that helps us to develop better gene therapy approaches. Also, with the increased understanding of molecular medicine, we have been able to develop more specific and efficient gene transfer vectors which are now producing clinical results.In this review, we will take a historical view and highlight some of the milestones that had an important impact on the development of gene therapy. We will also discuss briefly the safety and ethical aspects of gene therapy and address some concerns that have been connected with gene therapy as an important therapeutic modality. © 2013 Elsevier B.V.
Mechanical regulation of signaling pathways in boneWilliam R. Thompson | Clinton T. Rubin | Janet Rubin
Volume 503, Issue 2, July 2012, Pages 179-193
A wide range of cell types depend on mechanically induced signals to enable appropriate physiological responses. The skeleton is particularly dependent on mechanical information to guide the resident cell population towards adaptation, maintenance and repair. Research at the organ, tissue, cell and molecular levels has improved our understanding of how the skeleton can recognize the functional environment, and how these challenges are translated into cellular information that can site-specifically alter phenotype. This review first considers those cells within the skeleton that are responsive to mechanical signals, including osteoblasts, osteoclasts, osteocytes and osteoprogenitors. This is discussed in light of a range of experimental approaches that can vary parameters such as strain, fluid shear stress, and pressure. The identity of mechanoreceptor candidates is approached, with consideration of integrins, pericellular tethers, focal adhesions, ion channels, cadherins, connexins, and the plasma membrane including caveolar and non-caveolar lipid rafts and their influence on integral signaling protein interactions. Several mechanically regulated intracellular signaling cascades are detailed including activation of kinases (Akt, MAPK, FAK), β-catenin, GTPases, and calcium signaling events. While the interaction of bone cells with their mechanical environment is complex, an understanding of mechanical regulation of bone signaling is crucial to understanding bone physiology, the etiology of diseases such as osteoporosis, and to the development of interventions to improve bone strength. © 2012 Elsevier B.V.
Gender-specific methylation differences in relation to prenatal exposure to cigarette smokeSusan K. Murphy | Abayomi Adigun | Zhiqing Huang | Francine Overcash | Frances Wang | Randy L. Jirtle | Joellen M. Schildkraut | Amy P. Murtha | Edwin S. Iversen | Cathrine Hoyo
Volume 494, Issue 1, February 2012, Pages 36-43
Epigenetic alterations may mechanistically explain the developmental origins of adult disease, namely the hypothesis that many complex adult chronic diseases originate as a result of conditions encountered in utero. If true, epigenetically regulated imprinted genes, critical to normal growth and development, may partially mediate these outcomes. We determined the influence of in utero exposure to cigarette smoking on methylation at two differentially methylated regions (DMRs) regulating Insulin-like Growth Factor 2 (IGF2) and H19, and how this might relate to birth weight of infants born to 418 pregnant women. Smoking status was ascertained through self-report and medical records. Bisulfite pyrosequencing was used to measure methylation in umbilical cord blood DNAs. Least squares DNA methylation means at each DMR and birth weight were compared between infants of smokers and non-smokers, using generalized linear models. While there were no significant differences at the H19 DMR, infants born to smokers had higher methylation at the IGF2 DMR than those born to never smokers or those who quit during pregnancy (49.5%, SD = 8.0 versus 46.6%, SD = 5.6 and 45.8%, SD = 6.3, respectively; p = 0.0002). The smoking-related increase in methylation was most pronounced in male offspring (p for sex interaction = 0.03), for whom approximately 20% of smoking-related low birth weight was mediated by DNA methylation at the IGF2 DMR. Our findings suggest that IGF2 DMR plasticity is an important mechanism by which in utero adjustments to environmental toxicants are conferred. Larger studies to replicate these findings are required. © 2011 Elsevier B.V.
MicroRNA-146a is linked to pain-related pathophysiology of osteoarthritisXin Li | Gary Gibson | Jae Sung Kim | Jeffrey Kroin | Shunbin Xu | Andre J. van Wijnen | Hee Jeong Im
Volume 480, Issues 1-2, July 2011, Pages 34-41
Because miR-146a is linked to osteoarthritis (OA) and cartilage degeneration is associated with pain, we have characterized the functional role of miR-146a in the regulation of human articular cartilage homeostasis and pain-related factors. Expression of miRNA 146a was analyzed in human articular cartilage and synovium, as well as in dorsal root ganglia (DRG) and spinal cord from a rat model for OA-related pain assessment. The functional effects of miR-146a on human chondrocytic, synovialm and microglia cells were studied in cells transfected with miR-146a. Using real-time PCR, we assessed the expression of chondrocyte metabolism-related genes in chondrocytes, genes for inflammatory factors in synovial cells, as well as pain-related proteins and ion channels in microglial cells. Previous studies showed that miR-146a is significantly upregulated in human peripheral knee OA joint tissues. Transfection of synthetic miR-146a significantly suppresses extracellular matrix-associated proteins (e.g., Aggrecan, MMP-13, ADAMTS-5, collagen II) in human knee joint chondrocytes and regulates inflammatory cytokines in synovial cells from human knee joints. In contrast, miR-146a is expressed at reduced levels in DRGs and dorsal horn of the spinal cords isolated from rats experiencing OA-induced pain. Exogenous supplementation of synthetic miR-146a significantly modulates inflammatory cytokines and pain-related molecules (e.g., TNFα, COX-2, iNOS, IL-6, IL8, RANTS and ion channel, TRPV1) in human glial cells. Our findings suggest that miR-146a controls knee joint homeostasis and OA-associated algesia by balancing inflammatory responses in cartilage and synovium with pain-related factors in glial cells. Hence, miR-146a may be useful for the treatment of both cartilage regeneration and pain symptoms caused by OA. © 2011 Elsevier B.V.
Long non-coding RNA UCA1 regulated cell cycle distribution via CREB through PI3-K dependent pathway in bladder carcinoma cellsChen Yang | Xu Li | Yu Wang | Le Zhao | Wei Chen
Volume 496, Issue 1, March 2012, Pages 8-16
Long non-coding RNA urothelial carcinoma associated 1 (UCA1) promotes human bladder cancer cell proliferation, but the underlying mechanism remains unknown. After knocking down of UCA1 in BLZ-211 cells, several cell cycle-related genes (CDKN2B, EP300 and TGFβ-2) were screened by microarray assay and validated by real-time PCR. Interestingly, in western blot analysis, p300 (encoded by EP300) and its coactivator cAMP response element-binding protein (CREB) level were significantly down-regulated. Both suppression of UCA1 expression by shRNA in BLZ-211 cells and ectopic expression of UCA1 in UMUC-2 cells showed that UCA1 alteration paralleled to the expression and phosphorylation of CREB, and UCA1 obviously influenced AKT expression and activity. Furthermore, in BLZ-211 cells, cell cycle progression was greatly reduced after PI3-K pathway was blocked by LY294002, indicating that UCA1 affected cell cycle progression through CREB. Taken together, we concluded that UCA1 regulated cell cycle through CREB via PI3K-AKT dependent pathway in bladder cancer. © 2012.
The regulation of mRNA stability in mammalian cells: 2.0Xiangyue Wu | Gary Brewer
Volume 500, Issue 1, May 2012, Pages 10-21
Messenger RNA decay is an essential step in gene expression to set mRNA abundance in the cytoplasm. The binding of proteins and/or noncoding RNAs to specific recognition sequences or secondary structures within mRNAs dictates mRNA decay rates by recruiting specific enzyme complexes that perform the destruction processes. Often, the cell coordinates the degradation or stabilization of functional subsets of mRNAs encoding proteins collectively required for a biological process. As well, extrinsic or intrinsic stimuli activate signal transduction pathways that modify the mRNA decay machinery with consequent effects on decay rates and mRNA abundance. This review is an update to our 2001 Gene review on mRNA stability in mammalian cells, and we survey the enormous progress made over the past decade. © 2012 Elsevier B.V.
A current review of molecular mechanisms regarding osteoarthritis and painAndrew S. Lee | Michael B. Ellman | Dongyao Yan | Jeffrey S. Kroin | Brian J. Cole | Andre J. van Wijnen | Hee Jeong Im
Volume 527, Issue 2, September 2013, Pages 440-447
Osteoarthritis afflicts millions of individuals across the world resulting in impaired quality of life and increased health costs. To understand this disease, physicians have been studying risk factors, such as genetic predisposition, aging, obesity, and joint malalignment; however have been unable to conclusively determine the direct etiology. Current treatment options are short-term or ineffective and fail to address pathophysiological and biochemical mechanisms involved with cartilage degeneration and the induction of pain in arthritic joints. OA pain involves a complex integration of sensory, affective, and cognitive processes that integrate a variety of abnormal cellular mechanisms at both peripheral and central (spinal and supraspinal) levels of the nervous system Through studies examined by investigators, the role of growth factors and cytokines has increasingly become more relevant in examining their effects on articular cartilage homeostasis and the development of osteoarthritis and osteoarthritis-associated pain. Catabolic factors involved in both cartilage degradation in vitro and nociceptive stimulation include IL-1, IL-6, TNF-α, PGE2, FGF-2 and PKCδ, and pharmacologic inhibitors to these mediators, as well as compounds such as RSV and LfcinB, may potentially be used as biological treatments in the future. This review explores several biochemical mediators involved in OA and pain, and provides a framework for the understanding of potential biologic therapies in the treatment of degenerative joint disease in the future. © 2013 Elsevier B.V.
Therapeutic potentials of gene silencing by RNA interference: Principles, challenges, and new strategiesYan Deng | Chi Chiu Wang | Kwong Wai Choy | Quan Du | Jiao Chen | Qin Wang | Lu Li | Tony Kwok Hung Chung | Tao Tang
Volume 538, Issue 2, April 2014, Pages 217-227
During recent decades there have been remarkable advances in biology, in which one of the most important discoveries is RNA interference (RNAi). RNAi is a specific post-transcriptional regulatory pathway that can result in silencing gene functions. Efforts have been done to translate this new discovery into clinical applications for disease treatment. However, technical difficulties restrict the development of RNAi, including stability, off-target effects, immunostimulation and delivery problems. Researchers have attempted to surmount these barriers and improve the bioavailability and safety of RNAi-based therapeutics by optimizing the chemistry and structure of these molecules. This paper aimed to describe the principles of RNA interference, review the therapeutic potential in various diseases and discuss the new strategies for in vivo delivery of RNAi to overcome the challenges. © 2013 Elsevier B.V.
Association study of microRNA polymorphisms with hepatocellular carcinoma in Korean populationWon Hee Kim | Kyung Tae Min | Young Joo Jeon | Chang Il Kwon | Kwang Hyun Ko | Pil Won Park | Sung Pyo Hong | Kyu Seong Rim | Sung Won Kwon | Seong Gyu Hwang | Nam Keun Kim
Volume 504, Issue 1, August 2012, Pages 92-97
Background: Recent studies have suggested that common genetic polymorphisms alter the processing of microRNA (miRNA) and may be associated with the development and progression of cancer. Patients and methods: The association of miRNA polymorphisms with HCC survival was analyzed in 159 HCC patients and 201 controls by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results: The risk of HCC was significantly lower for the . miR-499A>G, AG. +. GG in HCC patients (AOR. =. 0.603, 95% CI. =. 0.370-0.984) and hepatitis B virus (HBV)-related HCC patients (AOR. =. 0.561, 95% CI 0.331-0.950). In addition, the risk of HCC was significantly lower for the . miR-149C>T, CT and CT. +. CC in HCC patients (CT; AOR. =. 0.542, 95% CI. =. 0.332-0.886, CT. +. CC; AOR. =. 0.536, 95% CI. =. 0.335-0.858) and HBV-related HCC patients (CT: AOR. =. 0.510, 95% CI 0.305-0.854, CT. +. CC: AOR. =. 0.496, 95% CI 0.302-0.813). The . miR-149C>T polymorphism was also associated with survival rate of HCC patients in OKUDA II stage. Conclusions: miR-149C>T and . miR-499A>G were associated with HBV-related HCC. Further studies on larger populations will need to be conducted to confirm these results. © 2012 Elsevier B.V.
Regulation of the MIR155 host gene in physiological and pathological processesTerry S. Elton | Helina Selemon | Shane M. Elton | Narasimham L. Parinandi
Volume 532, Issue 1, December 2013, Pages 1-12
MicroRNAs (miRNAs), a family of small nonprotein-coding RNAs, play a critical role in posttranscriptional gene regulation by acting as adaptors for the miRNA-induced silencing complex to inhibit gene expression by targeting mRNAs for translational repression and/or cleavage. miR-155-5p and miR-155-3p are processed from the B-cell Integration Cluster (BIC) gene (now designated, MIR155 host gene or MIR155HG). MiR-155-5p is highly expressed in both activated B- and T-cells and in monocytes/macrophages. MiR-155-5p is one of the best characterized miRNAs and recent data indicate that miR-155-5p plays a critical role in various physiological and pathological processes such as hematopoietic lineage differentiation, immunity, inflammation, viral infections, cancer, cardiovascular disease, and Down syndrome. In this review we summarize the mechanisms by which MIR155HG expression can be regulated. Given that the pathologies mediated by miR-155-5p result from the over-expression of this miRNA it may be possible to therapeutically attenuate miR-155-5p levels in the treatment of several pathological processes. © 2012 Elsevier B.V.
Contrapuntal role of ABA: Does it mediate stress tolerance or plant growth retardation under long-term drought stress?Nese Sreenivasulu | Vokkaliga T. Harshavardhan | Geetha Govind | Christiane Seiler | Ajay Kohli
Volume 506, Issue 2, September 2012, Pages 265-273
Recent developments in defining the functional basis of abscisic acid in regulating growth, development and stress response have provided essential components for its actions. We are yet to envision the impact of how differential levels of ABA influence plant growth across life cycle. Here we reviewed the information arising from the recent unprecedented advancement made in the field of ABA signaling operative under calcium-dependent and calcium-independent pathways mediating the transcriptional reprogramming under short-term stress response. Advancement made in the field of ABA receptors and transporters has started to fill major gaps in our understanding of the ABA action. However, ABA just not only regulates guard cell movement but impacts other reproductive tissue development through massive transcriptional reprogramming events affecting various stages of the plant life cycle. Therefore many questions still remain unanswered. One such intriguing question is the contradictory role of ABA known to mediate two opposite faces of the coin: regulating abiotic stress tolerance and imparting growth retardation. In this review, we critically assessed the impact of substantial elevated levels of ABA on impairment of photosynthesis and growth alteration and its subsequent influence on seed yield formation. Excess biosynthesis of ABA under stress may deprive the same precursor pool necessary for chlorophyll biosynthesis pathway, thereby triggering growth retardation. Further, we emphasized the importance of ABA homeostasis for integrating stress cues towards coordinating sustainable plant growth. Also we provided a pertinent background on ABA biosynthesis and degradation pathway manipulation to highlight the genes and processes used in genetic engineering of plants for changed ABA content. © 2012 Elsevier B.V..
Functional roles of effectors of plant-parasitic nematodesAnnelies Haegeman | Sophie Mantelin | John T. Jones | Godelieve Gheysen
Volume 492, Issue 1, January 2012, Pages 19-31
Plant pathogens have evolved a variety of different strategies that allow them to successfully infect their hosts. Plant-parasitic nematodes secrete numerous proteins into their hosts. These proteins, called effectors, have various functions in the plant cell. The most studied effectors to date are the plant cell wall degrading enzymes, which have an interesting evolutionary history since they are believed to have been acquired from bacteria or fungi by horizontal gene transfer. Extensive genome, transcriptome and proteome studies have shown that plant-parasitic nematodes secrete many additional effectors. The function of many of these is less clear although during the last decade, several research groups have determined the function of some of these effectors. Even though many effectors remain to be investigated, it has already become clear that they can have very diverse functions. Some are involved in suppression of plant defences, while others can specifically interact with plant signalling or hormone pathways to promote the formation of nematode feeding sites. In this review, the most recent progress in the understanding of the function of plant-parasitic nematode effectors is discussed. © 2011 Elsevier B.V.
The impact of transposable elements on eukaryotic genomes: From genome size increase to genetic adaptation to stressful environmentsBenoît Chénais | Aurore Caruso | Sophie Hiard | Nathalie Casse
Volume 509, Issue 1, November 2012, Pages 7-15
Transposable elements (TEs) are present in roughly all genomes. These mobile DNA sequences are able to invade genomes and their impact on genome evolution is substantial. The mobility of TEs can induce the appearance of deleterious mutations, gene disruption and chromosome rearrangements, but transposition activity also has positive aspects and the mutational activities of TEs contribute to the genetic diversity of organisms. This short review aims to give a brief overview of the impact TEs may have on animal and plant genome structure and expression, and the relationship between TEs and the stress response of organisms, including insecticide resistance. © 2012 Elsevier B.V.
Relationships between single nucleotide polymorphisms of antioxidant enzymes and diseaseAmanda Crawford | Robert G. Fassett | Dominic P. Geraghty | Dale A. Kunde | Madeleine J. Ball | Iain K. Robertson | Jeff S. Coombes
Volume 501, Issue 2, June 2012, Pages 89-103
The presence and progression of numerous diseases have been linked to deficiencies in antioxidant systems. The relationships between single nucleotide polymorphisms (SNPs) arising from specific antioxidant enzymes and diseases associated with elevated oxidative stress have been studied with the rationale that they may be useful in screening for diseases. The purpose of this narrative review is to analyse evidence from these studies. The antioxidant enzyme SNPs selected for analysis are based on those most frequently investigated in relation to diseases in humans: superoxide dismutase (. SOD2) Ala16Val (80 studies), glutathione peroxidise (. GPx1) Pro197Leu (24 studies) and . catalase C-262T (22 studies). Although the majority of evidence supports associations between the . SOD2 Ala16Val SNP and diseases such as breast, prostate and lung cancers, diabetes and cardiovascular disease, the presence of the . SOD2 Ala16Val SNP confers only a small, clinically insignificant reduction (if any) in the risk of these diseases. Other diseases such as bladder cancer, liver disease, nervous system pathologies and asthma have not been consistently related to this SOD SNP genotype. The . GPx1 Pro197Leu and . catalase C-262T SNP genotypes have been associated with breast cancer, but only in a small number of studies. Thus, currently available evidence suggests antioxidant enzyme SNP genotypes are not useful for screening for diseases in humans. © 2012 Elsevier B.V.
MicroRNA-195 and microRNA-378 mediate tumor growth suppression by epigenetical regulation in gastric cancerHongxia Deng | Yanan Guo | Haojun Song | Bingxiu Xiao | Weiliang Sun | Zhong Liu | Xiuchong Yu | Tian Xia | Long Cui | Junming Guo
Volume 518, Issue 2, April 2013, Pages 351-359
The epigenetic regulation of microRNAs is one of several mechanisms underlying carcinogenesis. We found that microRNA-195 (miR-195) and microRNA-378 (miR-378) were significantly down-regulated in gastric cancer tissues and gastric cancer cell lines. The expression of miR-195 and miR-378 in gastric cancer cells was significantly restored by 5-aza-dC, a demethylation reagent. The low expression of miR-195 and miR-378 was closely related to the presence of promoter CpG island methylation. Treatment with miR-195/miR-378 mimics strikingly suppressed the growth of gastric cancer cells whereas promoted the growth of normal gastric epithelial cells. In contrast, administration of miR-195/miR-378 inhibitors significantly prevented the growth of normal gastric epithelial cells. Expression of cyclin-dependent kinase 6 and vascular endothelial growth factor was down-regulated by exogenous miR-195 and miR-378, respectively. In conclusion, miR-195 and miR-378 are abnormally expressed and epigenetically regulated in gastric cancer cell lines and tissues via the suppression of CDK6 and VEGF signaling, suggesting that miR-195 and miR-378 have tumor suppressor properties in gastric cancer. © 2013 Elsevier B.V.
Diagnostic and prognostic value of circulating miR-21 for cancer: A systematic review and meta-analysisYang Wang | Xujie Gao | Feng Wei | Xinwei Zhang | Jinpu Yu | Hua Zhao | Qian Sun | Fan Yan | Cihui Yan | Hui Li | Xiubao Ren
Volume 533, Issue 1, January 2014, Pages 389-397
Background: MicroRNAs (miRNAs) have been reported to be aberrantly expressed in patients with cancer. Many studies have shown that circulating miRNAs could play potential roles as diagnostic and prognostic biomarkers of cancers. The aim of this meta-analysis is to summarize the role of circulating miR-21 as a biomarker in patients with a variety of carcinomas. Material and methods: Eligible studies were identified and assessed for quality through multiple search strategies. For diagnostic meta-analysis, the sensitivity, specificity, and other measures of miR-21 in the diagnosis of cancer were pooled using bivariate random-effects approach models. For prognostic meta-analysis, pooled hazard ratios (HRs) of circulating miR-21 for survival were calculated. Results: A total of 36 studies dealing with various carcinomas were included for the systemic review. Among them, 23 studies were finally enrolled in the global meta-analysis (17 studies for diagnosis and 6 studies for prognosis). For diagnostic meta-analysis, the overall pooled results for sensitivity, specificity, positive likelihood ratio (LRP), negative likelihood ratios (LRN) and diagnostic odds ratio (DOR) were 75.7% (95% CI: 67.1%-82.6%), 79.3% (95% CI: 74.2%-83.5%), 3.65 (95% CI: 2.83-4.70), 0.31 (95% CI: 0.22-0.43), and 11.88 (95% CI: 6.99-20.19), respectively. For prognostic meta-analysis, the pooled HR of higher miR-21 expression in circulation was 2.37 (95% CI: 1.83-3.06, P. <. 0.001), which could significantly predict poorer survival in general carcinomas. Importantly, subgroup analysis suggested that higher expression of miR-21 correlated with worse overall survival (OS) significantly in carcinomas of digestion system (HR, 5.77 [95% CI: 2.65-12.52]). Conclusions: Our findings suggest that circulating miR-21 may not suitable to be a diagnostic biomarker, but it has a prognostic value in patients with cancer. © 2013 Elsevier B.V.
Regulation of gene transcription by the oncoprotein MYCBernhard Lüscher | Jörg Vervoorts
Volume 494, Issue 2, February 2012, Pages 145-160
The proteins of the MYC/MAX/MAD network are central regulators of many key processes associated with basic cell physiology. These include the regulation of protein biosynthesis, energy metabolism, proliferation, and apoptosis. Molecularly the MYC/MAX/MAD network achieves these broad activities by controlling the expression of many target genes, which are primarily responsible for the diverse physiological consequences elicited by the network. The MYC proteins of the network possess oncogenic activity and their functional deregulation is associated with the majority of human tumors. Over the last years we have witnessed the accumulation of a considerable number of molecular observations that suggest many different biochemical means and tools by which MYC controls gene expression. We will summarize the more recent findings and discuss how these different building blocks might come together to explain how MYC regulates gene transcription. We note that despite the many molecular details known, we do not have an integrated view of how MYC uses the different tools, neither in a spatial nor in a temporal order. © 2011 Elsevier B.V.
Genetically engineered bacteria: An emerging tool for environmental remediation and future research perspectivesJay Shankar Singh | P. C. Abhilash | H. B. Singh | Rana P. Singh | D. P. Singh
Volume 480, Issues 1-2, July 2011, Pages 1-9
This minireview explores the environmental bioremediation mediated by genetically engineered (GE) bacteria and it also highlights the limitations and challenges associated with the release of engineered bacteria in field conditions. Application of GE bacteria based remediation of various heavy metal pollutants is in the forefront due to eco-friendly and lesser health hazards compared to physico-chemical based strategies, which are less eco-friendly and hazardous to human health. A combination of microbiological and ecological knowledge, biochemical mechanisms and field engineering designs would be an essential element for successful in situ bioremediation of heavy metal contaminated sites using engineered bacteria. Critical research questions pertaining to the development and implementation of GE bacteria for enhanced bioremediation have been identified and poised for possible future research. Genetic engineering of indigenous microflora, well adapted to local environmental conditions, may offer more efficient bioremediation of contaminated sites and making the bioremediation more viable and eco-friendly technology. However, many challenges are to be addressed concerning the release of genetically engineered bacteria in field conditions. There are possible risks associated with the use of GE bacteria in field condition, with particular emphasis on ways in which molecular genetics could contribute to the risk mitigation. Both environmental as well as public health concerns need to be addressed by the molecular biologists. Although bioremediation of heavy metals by using the genetically engineered bacteria has been extensively reviewed in the past also, but the bio-safety assessment and factors of genetic pollution have been never the less ignored. © 2011 Elsevier B.V.
The resurgence of Hormone-Sensitive Lipase (HSL) in mammalian lipolysisAntonis D. Lampidonis | Emmanuel Rogdakis | Gerassimos E. Voutsinas | Dimitrios J. Stravopodis
Volume 477, Issues 1-2, May 2011, Pages 1-11
The ability to store energy in the form of energy-dense triacylglycerol and to mobilize these stores rapidly during periods of low carbohydrate availability or throughout the strong metabolic demand is a highly conserved process, absolutely essential for survival. In the industrialized world the regulation of this pathway is viewed as an important therapeutic target for disease prevention. Adipose tissue lipolysis is a catabolic process leading to the breakdown of triacylglycerols stored in fat cells, and release of fatty acids and glycerol. Mobilization of adipose tissue fat is mediated by the MGL, HSL and ATGL, similarly functioning enzymes. ATGL initiates lipolysis followed by the actions of HSL on diacylglycerol, and MGL on monoacylglycerol. HSL is regulated by reversible phosphorylation on five critical residues. Phosphorylation alone, however, is not enough to activate HSL. Probably, conformational alterations and a translocation from the cytoplasm to lipid droplets are also involved. In accordance, Perilipin functions as a master regulator of lipolysis, protecting or exposing the triacylglycerol core of a lipid droplet to lipases. The prototype processes of hormonal lipolytic control are the β-adrenergic stimulation and suppression by insulin, both of which affect cytoplasmic cyclic AMP levels. Lipolysis in adipocytes is an important process in the management of body energy reserves. Its deregulation may contribute to the symptoms of type 2 diabetes mellitus and other pathological situations. We, herein, discuss the metabolic regulation and function of lipases mediating mammalian lipolysis with a focus on HSL, quoting newly identified members of the lipolytic proteome. © 2011 Elsevier B.V.
The impact of reactive oxygen species and genetic mitochondrial mutations in Parkinson's diseaseLi Zuo | Michael S. Motherwell
Volume 532, Issue 1, December 2013, Pages 18-23
The exact pathogenesis of Parkinson's disease (PD) is still unknown and proper mechanisms that correspond to the disease remain unidentified. It is understood that PD is age-related; as age increases, the chance of onset responds accordingly. Although there are no current means of curing PD, the understanding of reactive oxygen species (ROS) provides significant insight to possible treatments. Complex I deficiencies of the respiratory chain account for the majority of unfavorable neural apoptosis generation in PD. Dopaminergic neurons are severely damaged as a result of the deficiency. Symptoms such as inhibited cognitive ability and loss of smooth motor function are the results of such impairment. The genetic mutations of Parkinson's related proteins such as PINK1 and LRRK2 contribute to mitochondrial dysfunction which precedes ROS formation. Various pathways are inhibited by these mutations, and inevitably causing neural cell damage. Antioxidants are known to negate the damaging effects of free radical overexpression. This paper expands on the specific impact of mitochondrial genetic change and production of free radicals as well as its correlation to the neurodegeneration in Parkinson's disease. © 2013 Elsevier B.V.


text:
Swingers Club or 'Church:' Who Gets to Decide?
Goodpasture Christian School sits on a sprawling, bucolic campus seven miles north of downtown Nashville, where 900 students ready themselves for adult lives of college, career, and loving the Lord.
Right next door sits the United Fellowship Center, a planned church where adults will ready themselves to have sex with each other after enjoying a little BYOB togetherness.
It's the newest incarnation of The Social Club, a whispered-about swingers club in downtown Nashville that left for the suburbs when a building boom took its parking lot. The community went bonkers after zoning hearings revealed the club's plans to relocate in a former medical office building in Madison — adjacent to Goodpasture and within a mile of an Assemblies of God megachurch.
After months of debate, an emergency city zoning amendment and a state law designed to stop the relocation, the club's attorney made an announcement: The Social Club would open in its new location as a church.
Protection via the First Amendment effectively silenced zoning complaints — for now. But it sparked conversations about what it means for a secular organization suddenly to label itself a church, and religious scholars seem no more ready to plunge into that debate than American courts have proved to be.
"When I see this case, I do roll my eyes, but I also know Protestant Christians in America don't own 'church,'" said Kutter Callaway, assistant professor of theology and culture at Fuller Theological Seminary in Pasadena, Calif.

subset: commoncrawl


text:
6654-9792-6050-3214 is my code u like wfc games or just games period add me
6654-9792-6050-3214 is my code u like wfc games or just games period add me
dangelo have you added me
hey come join GS we have 30 members and are growing srronger everyday wwe have NO requirments to join we are a mwr and waw clan
if ya havent played with me on call of duty modern warfare reflex my friend code is (419104481360)
to tell u the truth im really crazy on mwr so you better watch out
i will never give up untill i know i accoplish my part and i know i tried my hardest and ill always give the enemy the biggest fight of there lives
IF YOU NEED A CLAN COME JOIN M|N JUST LET ME KNOW IF YA WANNA JOIN OUR CLAN WEBSITE IS www.ac-clanhq.tk I DO XP LOBBIES SO IF YA WANT A XP LOBBY PLZ GO THERE
Add me for Cod reflex:2689 3732 3144. give me ur friend codes.
Any1 wanna join my clan? http://codclandp.webs.com/ for more detai
My friend code is 481463740741
Call Of Duty Modern Warfare Reflex Friend Code


text:
It's because that relationship is so easily understood, so universal, that it has become a favored dramatic trope for filmmakers. While not every film about bosses takes place in classic corporate environs, each deals with power, its rewards and, more significantly, its risks.
THE GODFATHER (1972): It takes a truly charismatic actor to pull of a role like Don Vito Corleone. On one hand a generous benefactor and on the other a ruthless criminal willing to resort to significant violence, Marlon Brando's Corleone is a unique cinematic invention and the prototype for every movie crime boss that has followed.
NINE TO FIVE (1980): The worst-case scenario for corporate leadership, Dabney Coleman's Franklin Hart is, in the words of one of the secretarial pool trio that takes him on, "a sexist, egotistical, lying, hypocritical bigot". It comes as no surprise that Hart's is not a happy ending in this sharp satire on office politics.
FULL METAL JACKET (1987): Anyone who has spent any time in the military will tell you that the basic training drill instructor is the ultimate boss. Short of telling recruits when they may inhale and exhale, they are empowered with dictating everything their charges may and may not do. R. Lee Ermey's Gunnery Sgt. Hartman might be the ultimate cinematic representation of this relationship gone wrong. An instructor who wields discipline like a weapon, his desire to craft perfect Marines eventually leads to tragedy.
SWEET BIRD OF YOUTH (1962): The story of a failed actor who returns home to win his lost love is less a romantic melodrama than it is a study of a small town unofficially controlled by a powerful boss. How power is collected, curried and squandered in small town America is captured here.
THE PLAYER (1992): This pitch black comedy rates as one of legendary director Robert Altman's best and Tim Robbins' portrayal of a paranoid Hollywood executive is a scathing portrait of someone who finds that privilege comes with a price. What starts as a simple misunderstanding spirals into murder, betrayal and a true questioning of what is art and what is artifice.


text:
Tumblr App Clone
This project received 1 bids from talented freelancers with an average bid price of $1000 USD.Get free quotes for a project like this
Skills Required
Project Budget$1000 USD
Total Bids1
Project Description
Hello,nnI'm looking to clone Tumblr App ([url removed, login to view];hl=en) for Android and [url removed, login to view] project is basically divided in 2 parts: nn1. Clone Appn2. Customize the newly created app to match my own themennLet me know if you are interested on this [url removed, login to view]
Looking to make some money?
- Set your budget and the timeframe
- Outline your proposal
- Get paid for your work
Hire Freelancers who also bid on this project
Looking for work?
Work on projects like this and make money from home!Sign Up Now
- Forbes
- The New York Times
- Time
- Wall Street Journal
- Times Online


text:
Airport Pickups London (APL Cars) is a leading provider of London Airport taxi service. Reliable, comfortable and cheap Heathrow to Gatwick and Gatwick to Heathrow taxi transfers booked via our secure website, in just a few clicks.
Airport Pickups London is a leading provider of London airport minicab transfers. Our standards of reliability and comfort are second to none. What's more, we pride ourselves on offering the very best service and value for money.
Hundreds of customers from all over the world book their London airport car service with us on a daily basis. Our secure and simple-to-use website allows you to book your all-important Gatwick, Heathrow, Stansted, Luton and City airport taxi in just a few clicks. Whether you are arriving in London or heading off to any of the main London airports, contact us today for a comprehensive and competitive quote. We are happy to serve all major UK cities and destinations.
APL Cars serves all of the main London Airports and offers the cheapest and the smartiest way of travelling. Taxi journeys to/from Gatwick, Heathrow, Luton, Stansted and City Airports are just some of our most popular routes.
APL Cars is offers the best way to travel from heathrow airport, gatwick airport and stansted airport into London with airport cabs. We have transferred thousands of satisfied customers, our high proportion of repeat bookings testament to this. Our aim is to not only meet your expectations but to exceed them. From the moment you visit our website or speak to one of our friendly call centre team, you can be sure your Gatwick or Heathrow taxi is in safe hands.
If you have any questions at all about your London airport taxi, or if you'd simply prefer to speak with a human being, our call centre is staffed 24 hours a day, seven days a week. Call now on +44 (0)208 688 7744.
The vast majority of our cars are new and prestige models. Your comfort and safety are paramount to us. We want you to start or end your holiday or business trip on a high – that means top-notch cars are used for all our London airport taxi transfers.
If you have any questions at all about your London airport taxi, or if you'd simply prefer to speak with a human being, our call centre is staffed 24 hours a day, seven days a week. Call now on +44 (0)208 688 7744.
Our drivers are fully licensed, highly experienced and polite and courteous at all times.They know exactly where they are going and you can be certain your London airport pickup or drop-off is in capable hands.
Transparency is important to us. That's why when you contact Airport Pickups London for a limo quote, the price you are given is the final price you will pay.
An APL taxi from Heathrow to London starts from as little as £39. There won't be any nasty surprises upon arrival – you pay the amount you were originally quoted exactly.
What do we mean by comprehensive airport taxi fares? Airport Pickups London's service includes,as standard:
APL Cars offers a 24-hour online booking service, giving you the flexibility to book your Gatwick/Heathrow/Luton/Stansted taxi travel at your own convenience.
Our online booking system is fully secure with 128 Bit SSL. We understand the importance of financial security and we respect your privacy.
London is one of Europe's biggest transport hubs. We understand the importance of timing, that's why our cars are equipped with the latest in satellite navigation technology and our drivers know the quickest routes between the major airports. We will make sure your Heathrow to Gatwick taxi and Gatwick to Heathrow taxi transfer is on time. You can rely on Airport Pickups London to get you there safely and in plenty of time for your onward flight.
Prices for Gatwick to Heathrow and Gatwick to Heathrow start from as little as £35 for an airport shuttle service, please contact us for me details
We don't just specialise in London airport transfers, Airport Pickups London is also pleased to serve the major UK cruise ports. If you need to get to/from a London airport, residential or business address to cruise ports including Dover to Heathrow taxi, Southampton to Heathrow and Gatwick, Portsmouth to London, then contact us today for a competitive taxi quote or book online.
If you are travelling on business, or if you would simply prefer an even better class of London airport limo service, why not try APL Cars' 'Business Class' transfer service. Our VIP option includes:
Looking to set up a corporate account? Consolidate all your London airport taxi transfers bookings on one simple-to-use website; generate your own invoices; benefit from priority on all airport cab bookings; and manage your own/multiple bookings online.
Opening a corporate account with Airport Pickups London has never been easier. Find out more here


text:
Acts 3: 19 Therefore repent and be converted (restored), so that your sins may be wiped away, in order that times of refreshing (restoration) may come from the presence of the Lord;"
There is always an evaluation process when beginning a restoration project that looks at
1. Scope : What do we have to work with, to build upon.?
2. Vision: What do we want to get out of this life?
3. Goal: There has to a purpose, and that purpose is to bring Glory.
Check out our new Video format...
- McKesson, Dairyland Healthcare, Norton hospitalpresent
- Believers LIFE MinistriesPastor, present
- Empowered Lives (current)
- University of Kentucky, Spingfield SouthHigh
Word Breaker (WWF Cheat)
The premier Words With Friends cheat app! Word Breaker helps you play all of your favorite word puzzle games including Scrabble, Words with
Go places. Do challenges. Earn points. Unlock rewards. Play SCVNGR!SCVNGR is a game about going places, doing challenges and earning points.
Evernote Widget
**The latest version of Evernote is required in order to use the Evernote Widget app** The Evernote Widget lets you go directly to core Ever
SwiftKey 3 Keyboard Free
Unlock your typing potential. * Webby Awards People's Voice Winner for Innovation, 2012 * * Mobile World Congress Award Winner for Innovatio
TalkBack provides spoken feedback for eyes-free computingTalkBack is an Accessibility Service that helps blind and vision-impaired users int
ADWLauncher EX
ADWLauncher EX, beyond tweaking your android desktopHighly customizable home replacement application¿What's a home replacement application?
GO Launcher EX Notification
A plugin of GO Launcher EX to show number of missed calls, unread SMS and Gmail. GO Launcher Notification is an independent plugin of GO Lau
GS ADW Theme
READ ALL OF THIS FIRST! *Only for ADW Launcher *For help installing visit http://bit.ly/adwhelp *This is a ADW Launcher Theme with 200+ i
Partner Program - YouTube
Share your videos with friends, family, and the world
Frampton Comes Alive!
Shop Google Play on the web. Purchase and enjoy instantly on your Android phone or tablet without the hassle of syncing.
Top Android Apps and Games in the Android Market | AppBrain.com
Search the Android market and discover the best Android apps from AppBrain, the leading Android app directory.
Adele wrote much of the material on her sophomore album, 21, and her vocals and lyrics deliver from new depths, earning wide acclaim as one
SPB Shell 3D
SPB Shell 3D: next generation user interface. Enjoy your phone! If you are not able to launch SPB Shell 3D, please use "Home Switcher" appli
Application Folder - Apps on Android Market
Make pretty folders! This application provides folders in your home with pretty icons. Unlike default android folder, this is very easy and
Phone 2 Chrome - Apps on Android Market
Send links from Android to Chrome via Dropbox. Easy and fast. ** Menu OR Long press on url > share link > phone 2 chrome ** Phone 2 C
Folder Organizer lite - Apps on Android Market
Group items according to labels and create customizable folders and widgets Group items according to labels and create fully customizable fo
Qik Video for Atrix - Apps on Android Market
*Exclusively for Motorola Atrix* Qik Video Connect makes it easy to capture and share life moments right from your mobile phone through liv


text:
Sidux 2009-03: Debian Derivative Brings KDE 4.3.2
With Sidux 2009-03, code-named Momos, the Sidux project provides the next version of its distro based on Debian sid.
Sidux by name alone reveals its origin: the distro takes packages from Debian's unstable sid tree, patches them and thereby brings a distro to the market that is inherently stable. The newest verson bears the code-name Momos and is based on Linux kernel 2.6.31, which supports a slew of new hardware, among them USB 3.0 and X-FI sound cards. The desktop is now KDE 4.3.2, replacing KDE 3 and the Qt3 libraries.
Particularly challenging was integrating SysVinit, X.org and Insserv, whose developers were making countless changes to underlying components. Download from the project webpage will require some patience, as packages are currently not available. However, the ISOs are on the Debian project release page.
Issue 192/2016
Buy this issue as a PDF
Nextcloud Announces Raspberry Pi-Powered Home Cloud Box
Innovative system adds a hard drive and Ubuntu Core to the RPi for an IoT hub.
Linus Torvalds Confirms the Date of the First Linux Release
Linux is two weeks younger than we thought!
End of OpenOffice?
The Apache Software Foundation considers retiring OpenOffice
Adobe Gives New Life to NPAPI Plugin for Linux
Adobe won't kill the plugin in 2017
LinuxCon North America Convenes in Toronto, Canada
Linux Foundation's big event celebrates the 25th anniversary of Linux
Linux Turns 25
Linux has evolved from "won't be a professional" project to one of the most professional software projects in the history of computers.
Mirantis Joins Hands with SUSE To Offer RHEL Support; Red Hat Not Happy
Competitors get in the game with RHEL without Red Hat
Linux on Windows 10 Poses a Security Risk
Security researchers have already notified Microsoft; some fixes are available
Mirantis to Refactor OpenStack Fuel for Kubernetes
The company is collaborating with Google and Intel to use Kubernetes as an engine for Fuel
Canonical Joins Document Foundation Advisory Board
The upcoming release of LibreOffice will be available as a snap package


text:
|Home Software category Just in softwares Submit a program Contact us Link to us
Home > Desktop::Screen Savers: Seasonal > Easter Basket 3D Screensaver 3.0
Easter Basket 3D Screensaver 3.0
Free Easter Basket 3D Easter ScreensaverCompany: Scenic Reflections Screensavers
Platform: Win95,Win98,WinME,WinNT 3.x,WinNT 4.x,Windows2000,WinXP,Windows2003,Windows Vist
Size: 3627 KB
Price: USD $0.00
Release Date: 2007-05-15
Screenshot URL: http://www.scenicreflections.com/ithumbs/Easter_Basket_Screensaver.jpg
Category: Desktop::Screen Savers: Seasonal
Download the Free Easter Basket 3D Easter Screensaver from scenicreflections.com. The free Easter Basket 3D Animated Screensaver lets you enjoy Spring and the Easter holiday with a basket full of chocolate eggs just for you. Enjoy watching the butterflies flitter about and listen to the calming chirps of the birds in the distance all while enjoying the relaxing scenery. This free Easter screensaver features over 40 minutes of beautiful CD...
Related keywords:
free screensavers holidays easter easter basket 3d screensaver
Easter Basket 3D Screensaver Related Titles:Xmas Time 3D Screensaver 1.0.5 - This terrific 3D screensaver will help you feel the magic of the Holiday season.
3D Quiet Winter Screensaver 1.0.5 - Enjoy the amazing atmosphere of a quiet winter night with this screensaver!
Autumn Time 3D Screensaver 1.01.5 - Take a deep breath and smell the lush aroma of an autumn forest.
SaversPlanet Snowfall Screensaver 5.0 - If you like nature you will love this screensaver!
Halloween Gourd 3D Screensaver 1.0.5 - Halloween Gourd 3D Screensaver is our Halloween contribution to everybody.
Halloween Graveyard 3D Screensaver 1.0.5 - Halloween on your desktop!
Spring Flourish Free Screensaver 2.0.2 - Spring Flourish Free screensaver will show you trees in blossom on your desktop.
Saint Valentine's 3D Screensaver 1.01.7 - Saint Valentine's Day 3D Screensaver
Halloween Pumpkin 3D Screensaver 1.06 - Halloween Pumpkin 3D Screensaver is our Halloween contribution to everybody.
Xmas Holiday 3D Screensaver 1.0.6 - Turn it on and the holiday atmosphere of upcoming Christmas will surround you.
Spring Bloom Free Screensaver 2.0.2 - Look at your desktop and see the amazing views of the spring bloom on it!
Christmas Holiday 3D Screensaver 1.05 - Turn it on and the holiday atmosphere of upcoming Christmas will surround you.
Audio & Multimedia Business Communications Desktop Development Education Dwg Converter Games & Entertainment Graphic Apps Home & Hobby Network & Internet News Security & Privacy Servers System Utilities Web Development Others
Copyright © 2000 - 2016 FreeSharewareCenter.com - Freeware and Shareware Download Center.
All Rights Reserved.


text:
Contact Info: OC Beer Society (info@ocbeersociety) 949.891.2337
RateBeer: Your guide to world craft beer.
About Us
Terms Of Service
Privacy Policy
Add A Beer
Add A Brewer
Add A Place
Add An Event
Add Premium
Edit Profile
Sign out
Subscribe to our newsletter, RateBeer Weekly, a must for understanding new people, places and beers in worldwide craft culture.
2000-2016 © RateBeer, LLC. All Rights Reserved.
Privacy Policy | Terms of Service


text:
What are some other things you think husbands should stop doing? read more
Charisma Magazine
Page 43 of 127
Are You Willing to Live for Your Children?
If we are willing to go so far as to die for them, why is it that we often don't seem willing to live as a godly example for them? read more
Why Do Women Cheat?
As a follow-up article to "Why Do Men Cheat," here are some reasons why women have strayed from their husbands. read more
Why Do Men Cheat?
Here are some reasons—not justifications—why some men feel the "need" or desire to be unfaithful. read more
What Is the No. 1 Threat to Marriages?
You might think the answer to that question is infidelity. Think again. read more
7 Tips to Avoid Making Disastrous Family Decisions
Do you take these things into consideration when making choices that will affect your entire family? read more
10 Things to Teach Your Kids About Overcoming Failure
You can fail in a lot of areas in life, but here's why you can't fail with your children. read more
Why Fathers Need to Frequently Affirm Their Sons
When was the last time you gave your son any type of encouragement? Isn't it about time you did? read more
7 Ways to Make an Impression Upon Your Children
How often do you not only impress your children, but pierce them as well? read more
'Why Is My Daughter Cutting Herself?'
Unfortunately, this has become an issue in our culture. If you suspect your child or one you know that is doing this, here are some things of which to be aware before you seek help. read more
What Happens to Your DNA When Holy Spirit and Your Human Spirit Merge
The results are glorious.
A Word for Praying Prophets and Prophetic Intercessors
These intercessory assignments will not make sense in the natural, but are part of a strategic ...
Thousands Rally to Pray for Donald Trump
Believers responded overwhelmingly to a call for prayer and fasting for the Republican presidential ...
When the Holy Spirit Foils the Enemy's Destructive Plans
It wasn't a still small voice giving me an instruction. It was just an impression in my ...
5 Truths to Understand When Leading Leaders
If you are in leadership for any length of time, there will probably come a time where you will need to lead other leaders.
7 Reasons Your Organization Suffers from Marketing Malpractice
Receive Dr. Steve Greene's 7 Reasons Your Organization Suffers from Marketing Malpractice, plus 3 Things to Do Now to Prevent Further Damage as your FREE download.
Prophetic Word: The Angels God Has Sent to Fulfill Your Prophecy
"God wants me to encourage you that your breakthrough is imminent."
Early Warning Signs He Isn't God's Man for You
Asking these questions is vital if you want a Christ-centered marriage one day.
6 Warning Signs of Spiritual Danger
There are clear signs when a ministry has veered away from truth and into deception.
Prophecy: 'We Are in a Season of Supernatural Restoration'
"God is so big that even if He has to add extra years onto your life, He can do that."
What Every Spirit-Led Woman Ought to Know About Her Hormones
This OB-GYN with 20 years of medical practice has some helpful advice for both men and women.
What It Really Means to 'Grieve the Holy Spirit'
Many Christians have mishandled Ephesians 4:30.
When You Pray, This Will Guarantee You Foolproof Intercession
It really isn't all that complicated.
5 Truths to Understand When Leading Leaders
If you are in leadership for any length of time, there will probably come a time where you will need to lead other leaders.
Respected Assemblies of God Leader's Essential Secret to Success
During Dr. Charles Kelly's tenure as a superintendent in North Carolina, his district ...
Katherine Ruonala: Operating in Creative Miracles
The prophetess explains how God has equipped her for supernatural healing—and how you can ...
New Christian Film Leaves Pro-Choice Audience in Awe
The producers of Voiceless are hoping their critically acclaimed movie can help change the ...
What Many Christians Don't Understand About 'the Anointing'
God's anointing always serves a function. Discover that function in your life.
Avoid These Carnal Weapons When Dealing With Relationship Wounds
Satan feeds on the division fleshly weapons breed. Follow these biblical principles instead.
How You Can Break Strongholds for Permanent Freedom
You don't have to be controlled by demonic oppression, patterns of sin or by weaknesses in your ...
Resist Satan's Strategy to Lure You Into This Death Trap
Beware of these early warning signs of the devil's deception.
God's Power at Work in the Church Today
Charisma's founder, Steve Strang writes about today's hottest topics affecting the Christian faith.
When Believers Crucify Each Other in the Name of Discernment
There's a difference between a prophetic spirit and a critical spirit.
Continue Your Journey with the Holy Spirit
Follow God on the most fulfilling journey of your life with Encounter the Holy Spirit! Break away from past hurts and press into the One who wants a personal encounter with you.
Dream the Unimaginable In His Presence
Reach your full potential and be led by His Spirit. In His Presence takes you to a deeper understanding of who God is and how he communicates with us.


text:
Game by Game - Double K Breaks It Down
By Double K ,
Use your ← → (arrow) keys to browse more stories
You'll obviously get more detailed breakdowns of the games during the season, but just like I did last year, I wanted to give a quick game by game preview for the upcoming season.
Last year, I went 8-4 on my prediction for the 2007 season.
If you don't count the games the Razorbacks got screwed in, I went 8-0, and if they hadn't gotten screwed, I would have gone 12-0! I am awesome!
Without further ado, (now changing into my Slick Rick voice) heeerrre we go...
Arkansas vs. Western Illinois
Are you kidding me? WIU has about as much chance of winning this game as the Cubs do of winning the World Series this year... wait... change that to Devil Rays... wait, no... make it the Pirates! Anyway, Casey Dick completes his first 37 passes (including 5 TD passes) leading the Razorbacks to a 35-0 lead... after one quarter. He spends the second half on the bench resting his arm, and the Razorbacks begin the Petrino era 1-0 with a 105-6 win.
Arkansas vs. UL Monroe (in Little Rock)
Petrino gets his first glimpse of the Rock and thinks to himself "what a hell hole" and "we've got to stop playing games here." Anyway, Casey Dick raises his completion percentage from 97 to 98.5% after this game. He throws for seven touchdowns and Michael Smith rumbles for 200 yards on the ground as the Hogs win 97-3.
Arkansas at Texass
You know what they say about Texas! Only steers and queers come from there! Too bad they don't have any steers on their football team. For some reason, the Razorbacks enter the game a big underdog, but they pull off the "upset of the season" so far with a 54-14 spanking of the Shorthorns thanks in large part to great ball control and Alex Tejada's 11 field goals (six of which are over 50 yards).
Arkansas vs. Alabama
The Hammer Bammer Jammer Yellow Rammer Spammer boys got lucky last year that the game didn't come down to a Leigh Tifton extra point. Bama senior QB Jessica Parker Wilson has "one of those game" that causes liquor sales, the crime rate, and Bear Bryant tatoos to go up in Alabama. Jessica throws 5 picks and the Razorbacks get revenge from last year winning 47-21.
Arkansas vs. Florida
Welcome to Arkansas, Tim Teblow. The player that won the Heisman trophy in last year's ridiculous voting over Arkansas' Darren McFadden comes to town. Karma is a mother. Tebow, who only won the Heisman because of racist voters in the Northeast and West coast, gets crushed early and crushed often by our overlooked defensive line. Linebacker Elton Forte sends Tebow to the bench crying early in the third quarter with his fifth sack of the game, and the Razorbacks rally for another stunning win in Fayetteville 52-51.
Arkansas at Auburn
Goodness gracious, who doesn't love Tommy Tuberville? Me! That's who! You don't have Will Muschamp anymore whose defense limited Arkansas to only 7 points last year. You're getting it this year, Tuberville! And on your home turf! Petrino's master game plan works to perfection as Casey Dick picks apart the suspect Auburn defense and Arkansas native, Auburn QB Kodi Burns fumbles six times. Hogs win 63-10.
Arkansas at Kentucky
Another revenge game for the Hogs as Kentucky came in here last year with cross-dressing QB, Andre Woodson and somehow ended up with more points on the score board than the Razorbacks did at the end of the game. I'm claiming there was some sort of scoreboard malfunction that caused this, because no way in hell they were better than us last year. This year, they definitely are not. Revenge is sweet - 71-6 Hogs.
Arkansas vs. Ole Miss
No one around here really cares about this game at all. Some former Razorback coach strolls back into town, but this time he is with the worst SEC team in the league. Instead of anti-Houston Nutt banners flying around the stadium before the game, Razorback fans are flying banners and flags calling for Ole Miss to keep him forever. Razorback fans know that as long as Ole Miss has him as coach, that Ole Miss will be an automatic win every season. Round one to Petrino and company, 108-0.
Arkansas vs. Tulsa (Homecoming)
Offensive genius Gus Malzahn returns with his so-called high-flying Golden Hurricane offense for homecoming in Fayetteville. Well, he gets a lesson in offense this night as young paddawon Malzahn meets up with and tries to match offensive skillz with Darth Sidius Petrino. The master, Petrino, ends up cutting off both his legs and leaving him for dead by a boiling lava river. (Ok, so I took pieces from the movie that didn't quite go together. Sue me.) What I'm saying is that the master is still the master - Razorbacks 75 Golden Hurricane 60.
Arkansas at South Carolina
This was the game last season where D-Mac ran for about 4 miles on the soft Gamecock defense. Felix Jones had his way as well. This year Michael Smith will shred Spurrier's boys for about 300 yards and Casey Dick will manage nicely through the air as the Razorbacks win again 49-13.
Arkansas at Mississippi State
It's Croom and Doom time again at Mississippi State. Because of my love for the Croomer, this game will be close. The Hogs end up winning when the defense gets back to back safeties in the last minute of this contest to pull off a stunning win 31-30.
Arkansas vs. LSU (Little Rock)
Last year's triple OT win was an instant classic. D-Mac brought da wood and helped carry the Golden Boot back to Razorback country. I was in a casino playing poker watching this game last year and I plan to be doing it again this year. I'll be outplaying lesser opponents surely as Petrino will be outcoaching a lesser coach on the opposing sidelines. LSU's national championship hopes go down in flames as the Hogs win in 8 OTs this year 96-90.
(You ain't takin' our boot back!)
Double K's 100% unbiased final prediction: Hogs 12-0


text:
Your directions start from Patna, Bihar, India. And New Jalpaiguri, Haryana, India is the place you need to reach.
We are sure that after getting road driving directions from Patna to New Jalpaiguri will help you find the route from Patna to New Jalpaiguri easier! If you need more information on Distances, Travel Times, Flight Distances, Travel, please refer below-
Need to see distances summary for the driving directions? You can see the Distance from Patna to New Jalpaiguri!
Want to get bigger driving directions Map? Want to see the how the bigger picture looks? You can see the Map from Patna to New Jalpaiguri!
Want to rent a car for your journey? Get Estimated Patna Car Rental from Patna to New Jalpaiguri. We tell you the approximate Car Rental for different types of cars; for the road directions between Patna and New Jalpaiguri!
You wish to do a trip from Patna to New Jalpaiguri in your own vehicle? Check your fuel budget here using our Fuel Price Calculator, to estimate your trip cost. We have updated Petrol, Diesel, AutoGas and CNG prices across all major cities in India!
Wish you could just fly and jump from Patna to New Jalpaiguri than following directions? Check the Flight distance between Patna and New Jalpaiguri.
With the road route plan from Patna to New Jalpaiguri you might also want to know the Travel timeYou can find the the Travel Time from Patna to New Jalpaiguri. This will help you estimate how much time you will spend driving from Patna to New Jalpaiguri.
Need all the information above in a single view to plan your travel? Check how to Travel from Patna to New Jalpaiguri. Help yourself to better plan your travel from Patna to New Jalpaiguri.
Tired of travelling by road? You can just fly from Patna to New Jalpaiguri. Check the Flight Time from Patna to New Jalpaiguri before travelling.
Are you confused whether you should go by road or take a flight? Check How far is New Jalpaiguri from Patna and decide.
Are you travelling to place for first time? Are you not aware of the routes in that city? You can get the Patna to New Jalpaiguri Route planned from our Route planner.
Do you want to know how much would it cost to drive from Patna to New Jalpaiguri? Get to know how much would be the Trip Cost from Patna to New Jalpaiguri.*The directions given below are approximate. Road Conditions, Temporary Road diversions, Local on road events can affect directions!
© 2011-2016 distancesfrom.com. A softUsvista Inc. venture!


text:
Versace Kicks Off Paris Couture Week
Category: Media & Culture
/ Jan 21, 2013 11:03AM EDT
Paris couture week kicked off on Sunday (January 20) with Donatella Versace revealing her latest creations for Atelier Versace, the haute couture wing of the Italian label's fashion empire. Atelier Versace's Spring/Summer 2013 collection explored the relationship between architectural shapes and ethereal fabrics. Flowin g fabrics (chiffon and silk cady) contrasted nicely with the fitted dresses worn underneath. The overall silhouette was sleek and stream-lined with emphasized shoulders and a nipped-in waist. Designer Donatella Versace told Reuters that she had been inspired by the building where she chose to stage her show. "It all started when I came to see this place. I was speechless of the beauty and the architecture of the dome, of the glass, and the steel, iron because this woman tonight is about material and powerful and this is the place for me, what reminds me," she said. The collection was pure Versace in terms of its color palette- a mix of fluorescent neon yellow and pink hues sprinkled with black and gold numbers. This season, Atelier Versace aimed to render haute couture more modern by designing pieces that were wearable but still screamed luxury, such as the black pinstripe suits threaded with real 24-carat gold. Donatella Versace brought Atelier Versace back to Paris Haute Couture Fashion Week last year following an eight-year hiatus. "I'm allowed to do things that I cannot do for pret-à-porter, for example, I have two fabrics in this collection. One is black pinstripe, but the stripe is real gold. Another one is a denim, a Japanese denim, woven with real gold again. So you cannot do this kind of thing for example for pret-à-porter, it would cost a fortune. And these are special pieces, the clients of couture want something unique." Celebrities in attendance included Oscar winner Kevin Costner and Princess Charlene of Monaco. "It was very thoughtful [of Donatella Versace] to find me here in town and to make it possible... it's very generous. I mean a lot of people watching go, 'How do you get in the front row?' Um, I don't know, but we got that personal invite so we're flattered to be here," he said. Paris Haute Couture Week runs through Thursday (January 24).
Feb 19, 2016 1:53PM
Feb 19, 2016 12:52PM
Feb 19, 2016 12:44PM
Feb 19, 2016 12:33PM
Feb 19, 2016 12:32PM
Feb 19, 2016 11:53AM
Feb 19, 2016 11:32AM
Feb 19, 2016 10:58AM
Feb 18, 2016 10:47PM
Feb 18, 2016 9:52PM


text:
Making Decisions Using Managerial Accounting
Suppose you are a sales manager for Books on Wheels, Inc., which makes rolling book carts often used by libraries. The company is considering adding a new product aimed at university students. The new product will be a small, collapsible, wheeled tote designed specifically to aid students in transporting textbooks across campus.
1. List five questions you and the company would need to answer before proceeding with the development and marketing of this new product.
2. For each question identified in requirement 1, identify the information you would need to answer the question as well as the expected source of that information.
3. Identify three serious consequences of either not obtaining the information you need or obtaining inaccurate information.


text:
Java-Based Version of Lumines!
Java-Based Version of Lumines!
Jun 23, 2005 11:50PM PST
This is a HORRIBLE representation of the wonderful puzzle game that is Lumines for PSP. I know not everyone can afford a PSP and/or Lumines. This is for you guys. The music is half of the game! I am almost embarassed to give this link, knowing full well that most people will play it and dismiss Lumines as a bad or boring game. This is just a taste until you can get your hands on the real thing.
Comments (0)


text:
For over 50 years, A.Bliss has provided specialist dry mounting and framing services to the art world.
Read more about our heritage »
Our services
- Cold Mounting
- Hot Mounting
- Hot/Cold Sealing
- Archival Mounting
- Framing
- Supply of Mount & Foam Board
- Art Mover / Delivery Service
Cold mounting is the technique we use for the majority of prints that we work with. As you can tell from the name, it's a cold process using rollers and pressure sensitive adhesives. Please refer to our suitability chart for advice on recommended print types or our substrate chart to find the most suitable surface. Aluminium mounting / Dibond Mounting is the most popular choice when hanging without picture frames and Kapa mounting when fitting into frames.
Hot Mounting is predominantly used to mount fibre based prints, thick papers and old, distorted works onto cloth and other materials. We use our traditional 8ft x 4ft press that incorporates heat and pressure to mount prints.
This is the oldest and most proven mounting technique. We have retained the machinery and expertise to continue offering this service. This mounting technique is archival and reversible (depending on the substrate you choose) we can also mount much larger than the press size – please enquire.
We offer the option to seal your work. This can be matt/semi matt or gloss. These photographic seals protect against UV and light scratches or scuffs. They also make your work easier to clean. These can be used with hot or cold techniques please refer to our suitability chart to find out which types of print are recommended for sealing.
When the longevity and archival quality of prints is crucial, you have fewer options available. The one true archival mounting process is in a hot press (as this is reversible) on PH Neutral cotton rag museum or conservation board. This can however cause problems with larger prints not being rigid enough once mounted. We have developed ways around this and have other options for archival mounting.
Please enquire for details as often we can come up with a personalised solution.
Our expert picture framer has over 30 years of experience with a wide range of framing techniques including solid wood, spray painting and staining. As there are so many variables and things to consider when choosing your frame, we recommend you come in for a consultation. All our frames are bespoke and built in-house.
Its always best to bring at least one example of work with you, so our staff can colour match and best advise on the various framing options.
We supply a wide range of art boards including Foamboard, Kapa, Conservation Boards, Display Boards and Foamex. All are available cut to size or in stock size packs and with self adhesive coating.
We can always source things we don't have in stock. You can order by contacting us directly or pick up in person from our EC1 workshop. We also offer free, next day delivery in the London area for full packs.
We offer a pick up and delivery service to all our clients. Our experienced drivers use our dedicated art mover vans, which can take anything up to 3.3 meters long and 2 meters high. If your work is bigger, we can arrange trusted art movers for you.
Please contact firstname.lastname@example.com with any logistics enquiries
Recent Work...
The Eye Needs A Horizon – Bridget Smith
Frith Street Gallery, London, 22 Jan – 3 March 2016 "A series of large-scale cyanotype prints, Blueprint for a Sea. One of the earliest photographic processes (known as blueprints), the technique uses ultra-violet light to create two-tone representations on paper. This series of images have been created by photographing cinema seating, but also allude to […]Read more »
Susan Hiller – Lisson Gallery
Lisson Gallery, 13th November – 9th January 2015 This was Susan Hiller's debut exhibition at Lisson Gallery – it was made up of early works such as Painting Blocks (1970–84) and Another (1986), alongside more modern creations such as 'On the Edge (2015)'. The latter consists of 482 views of 219 British coastline locations in which […]Read more »
'T&RA' – Richard Galpin
Medium: sanded and varnished photograph, mounted in sections on 3mm Dibond Dimensions: 3.42 x 5.01 meters Building: 240 Blackfriars Architect: AHMM (Allford Hall Monaghan Morris) Consultant: Patrick Burrows / Art Source Image Mounting: A Bliss Framing & Installation: Darbyshire For this project, attention had to be paid to the size and weight of the final […]Read more »


text:
Honoring the memory of Nelson Mandela
"Sometimes it falls on a generation to be great. You can be that great generation. Let your greatness blossom," Nelson Mandela – a UNESCO Goodwill Ambassador - famously mused.
His call has special meaning, as we approach Nelson Mandela International Day (18 July), celebrated for the first time in 2010. The best way to honour his memory is to be "that great generation", by ensuring that his values are reflected in today's societies.
Mandela's life is an education to us all. His triumph over apartheid changed history, and is part of humanity's shared heritage. Arrested for anti-apartheid activism in 1962, Mandela was sentenced to life imprisonment following the Rivonia Trial.
"This was a turning point in the fight for freedom against the apartheid system in South Africa and a defining moment in the global struggle for human rights and dignity," said UNESCO Director-General Irina Bokova.
The records of the Trial and the Liberation Struggle Living Archive, including his defiant dock speech, are included in UNESCO's Memory of the World Register, dedicated to humanity's documentary heritage.
- Read the complete article
- Message from UNESCO Director-General on the occasion of the Nelson Mandela International Day, 18 July, 2014 (PDF)
<- Back to: Dynamic Content Single View


text:
Uniquely Qualified to Keep You Healthier For Life
Dr. Al Sears is the founder of the Center for Health and Wellness, a successful integrative medicine and anti-aging facility in Royal Palm Beach, Florida, with more than 25,000 patients. His cutting-edge therapies and reputation for solving some of the most difficult-to-diagnose cases attract patients from around the world.
As a graduate of the University of South Florida College of Medicine, Dr. Sears scored in the 99th percentile on his MCAT and graduated with honors in Internal Medicine, Neurology, Psychiatry, and Physical Medicine.
After entering private practice, Dr. Sears was one of the first to be board-certified in anti-aging medicine. As a pioneer in this new field of medicine, he is an avid researcher, published author, and enthusiastic lecturer.
Dr. Sears is board-certified as a clinical nutrition specialist and a member of the American College of Sports Medicine (ACSM), the American College for the Advancement in Medicine (ACAM), the American Medical Association (AMA), the Southern Medical Association (SMA), the American Academy of Anti-Aging Medicine (A4M), and the Herb Research Foundation, (HRF). Dr. Sears is also an ACE-certified fitness trainer.
Dr. Sears currently writes and publishes the monthly e-Newsletter, Confidential Cures, and daily email broadcast, Doctor's House Call, received by more than 500,000 subscribers. He also contributes to a host of other publications in the field. He has appeared on more than 50 national radio programs, ABC News, CNN, and ESPN.
Since 1999, Dr. Sears has published 16 books and reports on health and wellness with a readership of millions spread over 163 countries.
In his first book, The T-Factor, King of Hormones, Dr. Sears perfected the use of natural and bio-identical testosterone boosters to help men restore the drive, ambition, muscle strength, vitality and sexual performance of their youth.
Dr. Sears followed up with 12 Secrets to Virility, a full-blown strategy for male performance that includes his own patient-tested protocols for successfully dealing with men's health concerns like fighting excess estrogen, protecting the prostate, eliminating fat gain and keeping a sharp mind and memory.
In 2004, Dr. Sears was one of the first to fight against the conventional belief that cholesterol causes heart disease, proving that cholesterol is not the cause, but the part of the body that heart disease acts upon. In his third book, The Doctor's Heart Cure, Dr. Sears offers an easy-to-follow solution that effectively eliminates your risk of heart disease, high blood pressure and stroke.
In 2006, Dr. Sears shocked the fitness world by revealing the dangers of aerobics, "cardio" and long-distance running in his book, PACE: The 12-Minute Fitness Revolution. Expanding on the fitness principles in The Doctor's Heart Cure, he developed a fast, simple solution to restore muscle strength, guard against heart attack and burn excess fat. Today, PACE is practiced by thousands of people worldwide.
In 2010, Dr. Sears made history by bringing telomere biology to the general public. As the first U.S. doctor licensed to administer a groundbreaking DNA therapy that activates the gene that regulates telomerase, his breakthrough book Reset Your Biological Clock shows how anyone can preserve the energy of youth by controlling the length of your telomere, the true marker of aging.
An avid lecturer, Dr. Sears regularly speaks at conferences sponsored by the American Academy of Anti-Aging Medicine (A4M), the American College for Advancement in Medicine (ACAM), the Age Management Medicine Group (AMMG), and the Society of Anti-Aging, Aesthetic and Regenerative Medicine Malaysia (SAAARMM).
As the founder and director of Wellness Research Foundation, a non-profit research organization, Dr. Sears has made it his life's work to bring his patients the latest breakthroughs in natural therapies. As part of his ongoing research, Dr. Sears travels the world in search of herbs, novel cures and traditional remedies. Meeting with doctors and healers, Dr. Sears has brought back and revitalized much of the traditional knowledge considered endangered in today's modern world.
During an expedition to the Peruvian Andes, Dr. Sears brought back a nutrient-dense oil made from the Sacha Inchi nut, containing the highest plant source of heart and brain boosting omega-3 fatty acids.
In India, Dr. Sears studied at the oldest existing school of Ayurvedic medicine, the ancient Indian healing tradition, and was tutored by Ayurvedic doctors on the use of potent Indian herbs used to treat heart disease, cancer and Alzheimer's disease.
While trekking through the Amazon rainforest in Brazil, Dr. Sears lived among the native Ashaninka Indians, incorporating their ancient knowledge of healing herbs into his own nutritional supplement formulas.
In Jamaica, Dr. Sears met with the last living healer from the ancient and forgotten lineage known as the Maroons. Coming from West Africa 500 years ago, their knowledge was on the brink of extinction until Dr. Sears published a book showcasing their unique herbs and healing formulas.
On the island of Bali, Dr. Sears had a meeting with the most famous of the ancient healers known as "Balians," – Ketut Leyir – and also met two of the country's foremost herbalists. Dr. Sears is publishing a book showing how to use Balinese herbs and make unique healing mixtures for the skin and body.
In Africa, Dr. Sears traveled to seven different countries, met with the ancient Batwa people to learn their nearly-forgotten healing secrets, and visited with eight of Africa's most renowned herbal healers to study their use of powerful and little-known herbs that cure disease, including cancer.
With a life-long interest in botany, herbology, physiology and anthropology, Dr. Sears has a unique capacity to investigate the evidence behind the stories and claims of traditional medicine from native cultures around the world.
By exposing the flaws of mainstream medicine and pioneering new solutions through innovative approaches to exercise, nutrition and aging, Dr. Sears continues to empower the lives of his patients and readers through his books, newsletters and regular media appearances. Sign up today for a free subscription to his health newsletter, Doctor's House Call.
Here's what industry experts are saying about Dr. Sears:
"Dr. Sears blows away the conventional medical wisdom…"
-Ronald Klatz, MD, DO Founder and President, American Academy of Anti-Aging Medicine
"Dr. Sears has distinguished himself as a leader in the area of Anti-Aging Medicine and recently in cell and telomere biology…"
-Frederic J. Vagnini, MD, FACS; Medical Director of the Heart, Diabetes and Weight Loss Centers of NY
"Dr. Sears masterfully explains how we can flip the switches that allow access to the store of human vitality that resides within each one of us. We understood how to do this when we were young and now Dr. Sears gives us the evidence-based means to tap into our potency, strength, and stamina with intention."
-Paul L. Hester, MD, MBA
"Dr. Al Sears really gets it. What a refreshing breath of fresh air…"
-Jonny Bowden, author, Living the Low Carb Life: From Atkins to the Zone Choosing the Diet That's Right for You


text:
Georgia was the seventh least stressed state, with 37.7 percent of surveyed residents saying they felt stressed "a lot of the day." Hawaiians were the least likely to be stressed, with only 30.2 percent reporting stress, while Utah topped the list with 45.1 percent.
The poll was based on more than 350,000 interviews conducted monthly in 2010. Americans' average stress level was 39.4 percent.
Rural areas and more flexible family lifestyles helped keep stress low in Georgia, said Beth Nesmith, an assistant professor at the College of Nursing at Georgia Health Sciences University.
"The rural part means we have more flexible lifestyles to take care of family stresses," she said.
Georgia has fewer two-career families than many high-stress states in the West and Northeast, Nesmith said. One parent is more likely to stay at home in rural areas, she said.
Low stress levels cannot be attributed to Georgia being a more healthy state, Nesmith said.
"We are more unhealthy in terms of diet and diabetes," she said.
The economy might not be as significant a stress inducer. Stress levels did not increase much during the recession, according to Gallup-Healthways.


text:
This is an account of an occurence I had a summer or two ago with my very hot, sexy, and free-minded older sister, Denise. Let me begin by saying that she and I have always gotten along well, and, things of a sexual nature were never considered "off-
the family went to church like every other sun. morn. i laid in bed thinking of the nite before,my niece turned 18 and we through her a party.my niece has always been a small petite girl.the last couple years i noticed she was starting to develope in
Peggy's Story (Part 2)
Chapter Thirty One: Peggy's Story (Part 2)
During the time before I was supposed to go out to the farm, I did a lot of thinking about what had happened. There were a few times that I almost resolved to tell mom that I didn't want to go aft
Peggy's Story (Part 1)
Chapter Thirty: Peggy's Story (Part 1)
Miss Cramer turned toward me, snuggled closer in my arms and began her story, I grew up in Rawlings, Kansas and at first I guess my life was no different than most. But when I was a senior in high schoo
I slept with my neighbors wife
I went to my neighbors house again, becoming a daily routine it seems.
My neighbor Jim had some horses and we'd ride horses. We had an age differance.. I was 17 and they were in their late 40's early 50's.
I knocked on the door and his wife ans
That Next Summer…
First off this is a continuation of my first story, "When Home Alone at Summer" So go check that out too.
In the middle of July me and my sister are over at my cousin Kristen's house. The three of us used to be really close and we issed old
Peggy Cramer
Chapter Twenty Nine: Peggy Cramer
One evening when mom came back from one of her frequent Library Friends meetings, she brought a guest with her. When they came in, I was sitting in the kitchen having a snack. As they entered mom said, "Bill
uncontrolable desires
My name is rico, I havent been in Philli that long, just a little over a year. During this time I have met many of my cousins. We have really gotten close, where we would always be together, now we work at the same place. I have always admired my cou
Rain: Rain fucks her boss
It was an late midsummer morning. Rain had to get up and go to work at that hideous office, with all those guys there that stare at her like shes this huge bowl of ice creme. She couldn't understand why.She was 25 with long blonde hair that came down
Rain: Rain fucks her boss
It was an late midsummer morning. Rain had to get up and go to work at that hideous office, with all those guys there that stare at her like shes this huge bowl of ice creme. She couldn't understand why.She was 25 with long blonde hair that came down
My friend- the good samaritan:)
I haven't been in a relationship in over a year and as a result haven't had any form of sexual release other than masturbation which gets quite boring.
Recently, I was over at my friend John's house for Sunday football. Usually several o
Prom King and Queen are twins
It's the first day of twelth grade. All the girls love me, I have slept with a lot of them to. They love my 12 inch dick. Any way, i was Joking and said "I will fuck the prom queen if I am the king, no matter who she is." that was my mistake. you see
My Cousin Natalie
Chapter Twenty Two: My Cousin Natalie
My next sexual encounter took place a few months after my first experience with Amber, when my mother and I took a weekend trip to visit her sister, Vanessa. Aunt Vanessa and her husband Mark lived in a c
Helping to deceid the challange
We were over my cousin Debbie's house one evening, myself, my cousin Debbie, her friend Elaine and Elaine's boyfriend. The talk over some beers turned to talk about cocksucking, I was surprised as how openly the Women talked about it, as if we guys
She was alone after school what was i suposed to d
"For Home work tonight you will read pages 127 to 138, and answer questions 1-8 on page 139." Mrs. Smith said, she may be 40 years old but she always had my ateneion. She was about my height 5'6″. Long dark brown hair that came to the middle of her b
Badly needed releof
I was married about 15 months when we had our first child. Everything was great except the sex, none, I understood but even a hand job at home would have been nice.
One afternoon I was visiting my cousin Bernice at her house and chatting about m
Man Of My Dreams
His gray eyes pierced at me as I looked up at him. His hard cock was in my mouth and my lips were wrapped tightly around it, squeezing it. Soft moans escaped his mouth and his hands cupped my face while my head bobbed back and forth. I loved the feel
Caught in the act BY Anne
Caught in the Act !
By Anne
I was working in the mall at the kiosk selling candles . Bob stopped by and since I had no customers I didn't mind chatting with him . He wasn't the best looking guy around he was a bit over weight but t
As a kid I was always attracted to other boys but didn't really get any opportunities to act upon my lust. When I was 18 I was seduced by an older guy in his fifties and became his sex slave. He loved the fact that I was very small, skinny and childl


text:
Enjoy this article? You may also be interested in these related items from our partners
Disney Monsters, Inc. We Scare
Because We Care Girls T-Shirt
Limited Edition Monsters, Inc.
Jeweled Figurine by Arribas - Mike Wazowski
Monsters University
- Shake & Scare Mike
Courtesy of MoviePoster.com (via impawards.com), here is our first look at a teaser poster for Monsters, Inc. 3D which will make its theatrical debut on December 19, 2012.
The Academy Award®-winning animated comedy adventure Monsters, Inc. returns to the big screen—now in 3D—with lovable Sulley (voiced by John Goodman) and his wisecracking best friend Mike Wazowski (voiced by Billy Crystal) as they struggle to help a little girl named Boo (voiced by Mary Gibbs) find her way home from Monstropolis in the midst of a wild and mysterious cover-up.
Monsters, Inc. 3D will be followed by its prequel, Monsters University, which debuts theatrically on June 21, 2013.


text:
It's been an interesting last few days for the Angels. They got rid of hitting coach Mickey Hatcher Tuesday and Albert Pujols homered on Wednesday and Thursday.
Slap sticks?
Angels this season:
Last season the Angels were shut out 11 times, which was just slightly above average. This season they have been shut out eight times in their first 39 games. The only teams in the Live Ball Era (since 1920) to be shut out more than eight times at this point in the season are the 1984 New York Yankees (10), the 1976 San Francisco Giants (9) and the 1933 Boston Braves (9).
On the bright side, Mark Trumbo and Mike Trout are doing their part in ending the offensive hibernation. Trumbo and Trout are hitting .426 and .500 respectively this season in at-bats ending in curveballs, change-ups or sliders. During Trumbo's current eight-game hitting streak he is 16 for 28, with multi-hit games in four out of the last five.
Ervin Santana went five straight starts without a single run of support prior to winning his last two starts. Dan Haren has apparently caught whatever disease it was that Santana had during his losing skid. The Angels haven't scored in Haren's last three starts.
According to the Elias Sports Bureau, the last pair of starting pitchers to each have a streak of at least three games in which they received zero run support in a single season was Guillermo Hernandez and Mike Krukow of the 1980 Cubs.
Jered Weaver will look to bounce back from his horrific start against the Rangers as he opens the Angels' first interleague series of the year today against the light-hitting Padres. San Diego is last in the majors in slugging percentage (.331) and second-to-last in runs per game (3.2). The last time the Angels faced the Padres was in June of 2009 – a series in which Weaver capped a three-game sweep with a five-hit shutout.