text:
|Publication number
|Publication type
|Application number
|Publication date
|Nov 30, 1982
|Filing date
|Nov 26, 1980
|Priority date
|Nov 26, 1980
|Publication number
|Joe W. Gray, Terry W. Alger, David E. Lord
|Original Assignee
|The United States Of America As Represented By The United States Department Of Energy
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (5), Referenced by (114), Classifications (17), Legal Events (1)
|External Links: USPTO, USPTO Assignment, Espacenet
The invention described herein resulted from Contract No. W-7405-ENG-48 between the U.S. Department of Energy and the University of California.
The invention relates to an apparatus and method for sorting minute particles in a suspension fluid and more particularly to the fluid system for ultra high speed flow sorting. An example is an ultra-high-speed chromosome flow sorter.
There are many applications for apparatus capable of sorting minute particles entrained in a jet of a suspension fluid. Typically, the jet breaks up into droplets soon after emerging from the jet nozzle with only one particle in a droplet, and preferably only one particle in one out of 10 to 30 droplets to assure at most one particle per droplet. The criterion for sorting may be any particle characteristic that can be sensed in the jet before it breaks off into droplets. Once the characteristic of interest is sensed as a particle passes a sensing station, a decision is made to sort the droplet that will form with that particle. The sorting is accomplished by charging the droplet as it breaks off from the jet. Deflection plates down stream then cause the charged droplet to be deflected into a separate container. One important use of such a flow sorter is in the field of flow cytometry.
Flow cytometry has developed over the last two or three decades to automate counting body tissue cells in an aqueous suspension. Usually the cells have been stained to label the cellular component(s) of interest for quantitative analysis and interpretation. Briefly, the stained cells in the aqueous suspension are caused to flow under low pressure of about 10 psi through an optical sensing station. Once a particle has been sensed it can be counted and/or sorted. Typical sensing rates are 1000 cells per second, but to insure that each charged droplet contain only one cell, it is necessary to produce about 30 droplets for every cell flowing past the sensing station. Consequently, the typical droplet-production rate is 3�104 per second.
It would be desirable to increase the sorting rate by a factor of 10 to 50. The rate of electro-optic or other sensing could be increased by that much since photosensitive and electronic components are available for operation at pulse rates of from 106 to 109 per second. The problem is with the fluidic system, and more particularly the jet velocity at 10 psi and the droplet production rate of 3�104 per second are limiting factors. These have been the limiting factors in flow cytometry and sorting. A higher jet velocity requires a higher operating pressure, which compounds the problem of increasing the droplet rate, and the problem of coordinating the sort decision with the production of a charging pulse.
Although reference has been made to cells, the term has been used by way of example, and not with any intent to limit the invention to sorting only those particles that are the least structural aggregate of living matter capable of functioning as a living unit. The most likely use of this invention is in sorting mammalian chromosomes of a single type for investigations of the biochemical nature and chromosomal specificity of genetic lesions formed as a result of cellular exposure to mutogens or carcinogens. It was discovered at the Lawrence Livermore Laboratory in 1974 that chromosomes could be classified and purified to an unparalleled degree through flow sorting. The procedure is briefly as follows. Fluorescently stained chromosomes are suspended in an aqueous solution which is then surrounded by a sheath fluid in a nozzle so that the chromosomes are ejected one-by-one in a very small jet from an orifice. The chromosomes in the liquid jet pass through an intense laser beam. The resulting fluorescence from each chromosome is sensed and used to make a decision for sorting the droplets produced in response to the ultrasonic vibrations induced by a piezoelectric crystal driven at high frequencies.
An object of this invention is to provide a fluidic assembly for an ultra-high-speed flow sorter which operates about 10 to 50 times faster than existing instruments.
Another object is to provide a flow sorter capable of operating at pressures from about 250 psi to about 1000 psi.
Yet another object is to provide a new and improved jet nozzle which permits laminar flow rates well above the critical Reynolds number above which turbulent flow in a tube is expected.
Still another object is to provide a high intensity system for stroboscopic illumination of the droplet breakoff region to determine the proper position for a charge electrode in a flow sorter, and for the purpose of adjusting the timing of charge pulses following the sensing of a particle to charge droplets to be sorted as they break off from the jet.
These and other objects of the invention are achieved by using a fluid drive system of high pressure in the range of 250 to 1000 psi for greater flow velocity, a nozzle with an orifice having a small ratio of length to diameter for laminar flow rates well above the critical Reynolds number for the high flow velocity, and means for vibrating the nozzle along its axis at high frequencies in a range of about 300 kHz to 800 kHz. The orifice is preferably provided with a sharp edge at its inlet, and a conical section at its outlet for a transition from a short cylindrical aperture of small length to diameter ratio to free space. Sample and sheath fluids in separate low pressure reservoirs are transferred into separate high pressure buffer reservoirs through valve means which first permit the fluids to be loaded into the buffer reservoirs under low pressure and then subjected to high pressure. Once the buffer reservoirs are subjected to high pressure and valve means are operated to permit the buffer reservoirs to be emptied through the nozzle under high pressure, sheathed sample fluid flows through the nozzle and forms a jet at the outlet of the orifice. A piezoelectric crystal at the upper end of the nozzle chamber is driven by a voltage signal at a high frequency. This transmits pulsating forces along the axis of the nozzle to the sheath and sample, which then causes the jet to break up into droplets at the high frequency of the piezoelectric drive signal. However, the droplets are not formed for some finite distance after the jet exits the nozzle orifice. A cytometric sensing station is positioned at the exit of the nozzle, and a charging pulse is applied to the jet when a particle reaches a position further downstream where the droplets are formed so that a droplet containing a particle may be selectively charged as it separates from the jet. Deflection plates even further downstream are then effective to sort out the charged droplets.
In order to adjust the timing of charge pulses to be applied to the jet after sensing a particle to be sorted, the distance between the sensing station at the outlet of the nozzle and the droplet breakoff point is determined by stroboscopic illumination of the droplet breakoff region using a laser beam transmitted through means for breaking up the coherency of the laser beam and means for modulating the beam on and off. The breakoff point in the region thus illuminated may then be viewed, using a television camera and monitor, in order to determine the distance of the droplet breakoff point from the sensing station. Knowing the jet velocity and the distance of this point from the sensing station, the timing of a charging pulse can be so adjusted that it will occur as a particle sensed reaches the droplet breakoff point in order for the droplet formed with the particle to be sorted.
The novel features that are considered characteristic of this invention are set forth with particularity in the appended claims. The invention will best be understood from the following description when read in connection with the accompanying drawings.
FIG. 1 is a schematic diagram of a cytometer and flow sorter incorporating the novel features of the present invention.
FIG. 2 is an enlarged cross section of the tip of the nozzle in the system of FIG. 1 illustrating the configuration of the nozzle orifice.
FIG. 3 illustrates schematically a system for stroboscopic illumination of the droplet formation region in the system of FIG. 1 in order to position a charge electrode at the droplet breakoff point and adjust the timing of decision logic for sorting droplets.
FIG. 4 is a block diagram of an exemplary implementation of a sensor and decision logic for the system of FIG. 1.
Reference will now be made in detail to preferred embodiments of the invention, an example of which is illustrated in the accompanying drawings.
Referring to FIG. 1, an ultra-high-speed flow sorter according to the present invention will now be described with reference to sorting fluid-suspended particles which may be chromosomes in a biological sample. The sample is taken from a vial 10 and transferred into a coil 12 of high-pressure teflon tubing via a two-way valve 14 under low pressure (typically 10 psi) provided by nitrogen, or other inert gas, introduced into the vial 10 from a source (not shown). A second two-way valve 16 connects the output of the sample coil 12 to a waste container 18. Once a portion of the sample flows into this waste container, the low-pressure gas into the vial is cut off, or otherwise removed, leaving the coil filled with a predetermined volume of sample to be run through a nozzle 20. The coil thus serves as a buffer reservoir. In practice, at least one additional sample coil would be provided in order to be able to switch from processing one sample to the other with a minimum of interruption.
Before, or after, loading the sample coil 12 in preparation for a run, a vessel 22 is filled with a sheath fluid from a vial 24, or other container for sheath fluid. To accomplish that, a two-way valve 26 is set for transfer of fluid from the vial 24 under the force of low pressure (typically 10 psi) nitrogen. A two-way valve 28 connects the output of the vessel 22 to a waste container 29, for this transfer operation to allow the vessel 22 to be filled with sheath fluid. Then the low-pressure source of nitrogen is removed from the vial 24. The vessel 22 is thus loaded to function as a buffer reservoir for the sheath fluid.
To process the sample from the coil 12 through an orifice 30 in the nozzle with a sheath of fluid from the vessel 22, the valve 28 is first turned to connect the vessel 22 to the nozzle 20. Then the valve 26 is set to connect a source (not shown) of high-pressure nitrogen, or other inert gas. For purposes of example, the high pressure is illustrated as 800 psi, but may be from about 250 psi to about 1000 psi. This will cause the sheath fluid to fill the nozzle. Once the nozzle is full of sheath fluid, the valve 14 is set to connect the source of high-pressure gas to the coil 12, and the valve 16 is turned to connect the reservoir 12 to the nozzle 20. Sample fluid is thus caused to flow out of a tube 32 in the nozzle.
A spacer 34 holds the tube 32 in alignment with the axis of the nozzle and its orifice 30. Passages in the spacer permit the sheath fluid to flow downwardly, through a cylindrical section 20a and a conical section 20b. This allows the sheath fluid to completely surround the sample fluid as the sample fluid emerges from the tube 32. Both are under about the same high pressure and will flow down through the conical body 20b to the orifice 30 with laminar flow (i.e., nonmixing flow). In practice, the pressure of the sheath fluid is adjusted to be slightly higher than the pressure of the sample fluid to so constrict the diameter of the sample fluid stream as to assure that particles are caused to pass out of the orifice 30 one at a time. Generally, the greater the sheath pressure, the smaller the sample fluid diameter in the nozzle between the output of the tube 32 and the orifice 30 of the nozzle.
The diameter of the nozzle, both in the cylindrical section 20a and the conical section 20b, is so large in comparison to the diameter of the orifice that the flow of sheathed sample may be regarded as being from a very large container of unlimited volume into a small diameter orifice or tube. The orifice is shown enlarged in FIG. 2.
At the orifice, there is an abrupt transition from the relatively large diameter cross section of the conical section 20b to a very small diameter of the orifice 30, typically 0.1 to 4 mils, but the coordination of laminar flow outside and inside the orifice is not disturbed. This is for the reason that the diameter of the orifice is so small that it is like an inlet of a tube connected to a large container of infinite volume, as just noted above. Potential flow theory holds that the inlet is the center for spherical areas of equipotential, represented in FIG. 2 by semicircles. Radius lines eminating from the center of the inlet represent the direction of flow from different spherical areas with equal velocity. As the different spherical areas approach the inlet, they transform into small planar areas of equipotential with a corresponding increase in velocity and with no disturbance in the laminar flow.
This principle of "hydrodynamic focusing" is commonly used in flow cytometers to transform a large stream into a narrow stream, but it has not heretofore been used in flow cytometers at high pressures. Conventional systems operate at about 10 psi. Any significant increase in pressure, such as 300 psi, could not be expected to work stably. This is because, for laminar flow, the Reynolds number, R, must be below a critical value, Rc, of 2300. The Reynolds number is given by the fluid flow relationship:
v=average flow velocity
d=tube diameter,
ρ=fluid density, and
η=fluid viscosity coefficient.
For a 70 μm orifice (i.e., d=70 μm) at an average flow velocity, v, of 80 m/sec, the Reynolds number is typically 5600, well above the critical Reynolds number. Consequently, turbulent flow, and not laminar flow, could be expected, yet turbulent flow has been avoided by the present invention even though the critical Reynolds number is exceeded due to high pressure, which may be as high as from 250 psi to 1000 psi. This has been achieved by maintaining the ratio of the length, L, of the orifice to its diameter, D, small (close to, and preferably less than 1), as shown in FIG. 2 where the length is measured to the end of the constant diameter portion of the orifice 30. Specific examples of nozzles (commercial jewel 51 μm orifices) that have been tested at about 800 psi are as follows:
______________________________________ L/D L/D*______________________________________1 0.75 1.752 2.14 2.623 0.65 1.15______________________________________ *Before grinding down L.
These examples are drastically improved over the same orifices with a larger L/D ratio before grinding, both to reduce L and to provide a sharper inlet corner. And the performances of the first and third samples were superior to the performance of the second. It can therefore be concluded that for the present invention, the L/D ratio should be close to one, not more than about 2, and preferably less than 1. A conical portion of the orifice at the outlet side allows smooth transition from a small diameter to an infinite diameter of free space.
At the outlet of the orifice, a jet is formed of the sample encased in a sheath fluid. The diameter of the sample in the jet is very small, relative to the diameter of the jet, and so small that only one fluid suspended particle will flow out of the nozzle at a time. If the jet is then allowed to break into droplets, any one droplet will not contain more than one particle. A piezoelectric crystal 40 is driven by a high frequency signal (300 kHz to 800 kHz) from a source 42 to form droplets at the high frequency of the drive signal. The ultrasonic vibrations created by the piezoelectric crystal are transferred to the liquid jet through the nozzle 20 and the sheath fluid which fills it. Due to the much higher velocity of the jet stream, higher vibration frequencies can be used to form droplets at a higher rate.
As the jet emerges from the nozzle, and before it breaks up into droplets, the presence of the particle of interest is detected, and the particle detection function of a conventional unit 43, labeled "sensor and decision logic," is used for counting and sorting the detected particles. The type of sensor used depends upon the nature of the particle. There is always some property (electrical, optical or other characteristic) of the particles that allows its detection by a suitable sensor.
Assuming fluorescently stained chromosomes for the particles, the jet is illuminated by an intense beam from a CW laser 44. The resulting fluorescence from each chromosome is sensed and used to select droplets containing the chromosome for sorting by the unit 43. This unit may also contain a counter for determining the number of particles sorted, as will be described with reference to FIG. 4.
Once the presence of a chromosome is detected, the decision logic pulses the jet, either directly through the tube 32, or indirectly through an annular electrode 46 which surrounds the jet. A large negative charge is placed on the droplet then being formed as it breaks off from the jet. The charged droplet is then deflected by plates 48 and 49, thereby causing the chromosome to be collected in a container 50. The unselected droplets (i.e., droplets that are not thus charged) fall into a waste container 52. It can be readily appreciated that the combination of maintaining the fluidic system at a high pressure (exceeding 250 psi), and the jet at a high frequency of vibration (exceeding 300 kHz), provides a sorting rate of 3�105 per second which is ten times higher than the typical rate of 3�104 per second heretofore available. A higher pressure and vibration frequency will produce even higher sorting rates.
In order to adjust the timing of the decision logic 43 to pulse the electrode 46 as a selected droplet is being formed, it is desirable to be able to determine the droplet breakoff point, or more specifically to determine the distance from the sensing station at the exit of the nozzle to the breakoff point, in order that a proper delay may be adjusted in the decision logic for generating the pulse applied to the charging electrode for the particular jet velocity. The pulse applied to the charging electrode may then be synchronized to occur in time coincidence with the formation (breakoff) of a droplet based on the distance to the breakoff point and the velocity of the jet. The velocity, which is a function of pressure for the particular nozzle design, is predetermined, so it is then necessary to determine only the distance. This is usually accomplished by stroboscopic viewing of the droplet breakoff point, using a light emitting diode (LED) and a television camera. However, LED's do not emit sufficient light when operated at high frequencies (above about 250 kHz). Consequently, for the present invention, a laser is used for a strobe light effectively triggered at a 10 kHz to 1 MHz, as shown in FIG. 3.
Referring now to FIG. 3, a laser (e.g., HeNe laser) 60 is switched by an acousto/optic modulator 62 from a position away from a pinhole 64 in an opaque plate 66 to a position in line with the pinhole. This modulator 62 is comprised of an optically transparent crystal which has a variable periodic optical index of refraction controllable by internally transmitted acoustic waves produced by electrical impulses applied to the crystal. The excitation signal for the modulator is derived from the signal applied to the piezoelectric crystal 40 (FIG. 1) via a variable delay element 68. This variable delay is adjusted to synchronize modulation of the laser beam onto the droplet breakoff region of the jet with droplet formation in order to be able to strobe the formation of droplets as picked up by a television camera 70 and displayed on a television screen 72. A rotating frosted lucite cylinder 74 is placed in the path of the laser beam passed through the pinhole to destroy the coherency of the laser beam and thus avoid speckle that would otherwise occur (due to the coherency of the light) and obscure the droplets on the TV screen.
This arrangement produces a very intense droplet illumination system that operates well even at 800 kHz. The strobe effected in this manner permits stopping the droplets in space as they form. This enables the operator to determine the distance to the breakoff point, and hence the delay to be used in generating a charge pulse at a time which exactly corresponds to the time a droplet having a particle just sensed reaches the droplet breakoff point. In that manner, a particle is sensed in the jet stream and a delayed charge pulse is generated at the moment the particle reaches the droplet breakoff point.
During this procedure for determining the distance of the breakoff point from the sensing station at the outlet of the nozzle, the charge electrode must be removed in order that the television camera view the breakoff point. Consequently, a preferred arrangement for charging the droplets as they break off obviates the need for a charge electrode by applying the charging pulse to the tube 32. For this purpose, the nozzle is made of plastic so that a voltage can be put on the jet for the duration of the pulse. A droplet which then breaks off during the presence of the charging pulse will remain charged throughout its free fall into the container 50. The delayed charging pulse may be produced by the particle detection circuit through a variable monostable vibrator (one-shot circuit) as shown in FIG. 4.
Referring now to FIG. 4, an exemplary sensor and decision logic 43 is comprised of: a photodetector 80, amplifier 82, and a threshold detector 84 for the sensing and decision function; and a variable monostable multivibrator (one shot) 86 and a pulse generator 88 for the sorting function. The threshold detector eliminates noise below a predetermined level and allows a signal pulse produced by a sensed particle (via the photodetector 80) to trigger the one shot 86. The trailing edge of the one shot then triggers the pulse generator 88. In this example, the decision to generate a pulse is based solely on the amplitude of a light pulse due to fluorescence of a particle passing through the laser beam. Other characteristics sensed in other ways, and other criteria, could be used in similar or analogous ways for the sensing and decision functions. For each electrode charging pulse generated, a counter 90 is incremented by one in order to have a count of all particles sorted. This counter is reset at the start of each run by applying a rest pulse.
It should now be apparent that an ultra-high-speed flow sorter is provided wherein a sample of particles suspended in a fluid under high pressure in excess of 250 psi is sheathed with a fluid at the same high pressure and expelled as a jet through a nozzle vibrated at high frequency in excess of 300 kHz to form droplets at the rate of nozzle vibration. The average flow velocity of the fluid through the nozzle orifice is so high that the critical Reynolds number for laminar flow is exceeded, yet laminar flow is maintained due to a small length to diameter ratio of the orifice. A pressure buffer between the high pressure part of the system and the low pressure sheath and sample sources facilitates use of very high pressures. In order to adjust the timing of charging pulses for sorting the droplets, a high intensity laser beam is used to illuminate the breakoff region of the droplets, and strobed (modulated) to stop the droplets in space as they break off. To remove laser speckle (due to the coherency of the light) so the droplets can be seen, a rotating frosted lucite cylinder is placed in the beam path which destroys the coherency of the light.
Although particular embodiments have been described and illustrated herein, it is recognized that modifications and equivalents may readily occur to those skilled in the art. Consequently, it is intended that the claims be interpreted to cover such modifications and equivalents.
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|Jun 14, 2016
|Xy, Llc
|System and method for sorting cells
|Apr 10, 2015
|Jun 28, 2016
|Inguran, Llc
|Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm
|Mar 14, 2013
|Aug 2, 2016
|Inguran, Llc
|Nozzle assembly for a flow cytometer system and methods of manufacture
|Feb 11, 2013
|Aug 23, 2016
|Xy, Llc
|System and method for sorting cells
|May 18, 2001
|Nov 13, 2003
|Buchanan Kris S.
|Rapid multi-material sample input system
|Nov 1, 2002
|May 6, 2004
|Lary Todd P.
|Monitoring and control of droplet sorting
|Nov 21, 2002
|Dec 9, 2004
|Jan-Erik Ekstrom
|Method of steriling a packaging material by means of a sterilising agent in liquid form
|Feb 28, 2005
|Jul 14, 2005
|University Of Washington
|Droplet formation systems for flow cytometers
|Feb 22, 2005
|Jul 21, 2005
|Larson Bradley J.
|Methods and apparata for precisely dispensing microvolumes of fluids
|Apr 6, 2005
|Aug 25, 2005
|Cytonome, Inc.
|Method and apparatus for sorting particles
|Mar 31, 2006
|Nov 16, 2006
|Dako Colorado, Inc.
|Systems for transiently dynamic flow cytometer analysis
|Sep 22, 2006
|Feb 5, 2009
|Sumitomo Electric Industries, Ltd.
|Grain Classifying Device And Adhesive Containing Grains Classified By The Device
|Jul 27, 2005
|Nov 19, 2009
|George C Malachowski
|Enhancing Flow Cytometry Discrimination with Geometric Transformation
|Feb 11, 2010
|Cytonome/St, Llc
|Method and apparatus for sorting particles
|Aug 7, 2009
|Jun 3, 2010
|Cytonome/St, Llc
|Method and apparatus for sorting particles
|Jun 17, 2010
|Oct 7, 2010
|Sumitomo Electric Industries, Ltd.
|Grain classifying device and adhesive containing grains classified by the device
|Jan 13, 2011
|Cytonome/St, Llc
|Method and apparatus for sorting particles
|Sep 15, 2011
|Neckels David C
|Calculate Drop Delay for Flow Cytometry Systems and Methods
|Nov 15, 2012
|Fujifilm Dimatix, Inc.
|Imaging Jetted Ink
|Mar 9, 2011
|Nov 21, 2012
|Systems and methods for calculating drop delay of a flow cytometry
|Mar 9, 2011
|Dec 24, 2014
|Systems and methods for calculating drop delay of a flow cytometry
|May 10, 2012
|Mar 19, 2014
|Imaging jetted ink
|Sep 14, 1994
|Apr 19, 1995
|Toa Medical Electronics Co., Ltd.
|Particle analyzing apparatus
|Nov 25, 1996
|Jul 2, 1997
|Siemens Aktiengesellschaft
|Method and device for the observation of drop formation at the break-off point in a liquid jet
|Nov 21, 1986
|Jun 4, 1987
|Liquid Air Corporation
|Method for measurement of impurities in liquids
|Apr 14, 1992
|Oct 29, 1992
|Amersham International Plc
|Method for forming an array of biological particles
|Oct 13, 1995
|Apr 25, 1996
|University Of Washington
|High speed flow cytometer droplet formation system
|Oct 13, 1995
|Aug 1, 1996
|Univ Washington
|High speed flow cytometer droplet formation system
|Jul 10, 1998
|Jan 21, 1999
|Particle Measuring Systems, Inc.
|Particle detection system utilizing an inviscid flow-producing nozzle
|Jun 25, 1999
|Feb 3, 2000
|Coulter International Corp.
|Flow cytometer droplet break-off location adjustment mechanism
|Mar 9, 2011
|Sep 15, 2011
|Beckman Coulter, Inc.
|Calculate drop delay for flow cytometry systems and methods
|Sep 19, 2013
|Mar 27, 2014
|Inguran, Llc
|Flow cytometer nozzle tip
|U.S. Classification
|256/23, 209/638, 209/906, 239/102.2, 209/546, 239/4, 209/3.1, 209/551, 209/579, 209/939
|International Classification
|Cooperative Classification
|European Classification
|Feb 13, 1981
Owner name: UNITED STATES OF AMERICA AS REPRESENTED BY THE UNI
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNORS:GRAY JOE W.;ALGER TERRY W.;LORD DAVID E.;REEL/FRAME:003829/0217
Effective date: 19801030


text:
From the Back Cover
Did you realize that the average new parent snaps at least 10 photos a day? That a family vacation can yield a thousand images? Of course you did — odds are, you've been there. And now, you're drowning in digital photos and can't find the one you want.
Here's how to choose and use software that brings order to your digital chaos. No matter how many images you already have, this book helps you get them under control — for good.
Understand the different types of software and learn to make it work for you
Compare database and browser-based storage systems
Learn the best way to download images
Discover the quickest ways to search for specific photos
Find out the best, easiest, and safest backup methods
Get special tips on traveling with your camera gear
Explore safe ways to share photos

subset: commoncrawl


text:
Open Access Publishing and REF
For all University of Portsmouth Research and Academic Staff
Will your publications be eligible for the next REF? For the next REF HEFCE require journal and conference articles (with an ISSN) accepted for publication to be open access. Dr Emily Bennett, the University's Research Outputs Manager, will take researchers through the different types of open access models and the steps researchers need to take to ensure their research outputs comply with the requirements.
This workshop will enable you to:
Understand the different open access routes
Understand what you need to do upon acceptance of your article to comply with university policy on open access
- Date and Time
- Location
Purple Door Boardroom
28 Guildhall Walk
Portsmouth, United Kingdom
None yet
- Settings
Whoops! Couldn't connect
- Settings
Friends Who Are Going
Connect to Facebook


text:
Your Casting Ideas
Hello gang,
It takes a village to raise a movie, I always say. It's a passionate and often times long process. We've had a lot of industry support over the... Read more
Quick check in
It's been awhile since we've updated so just wanted to give you all an idea of where we are and what's been happening. So we obviously have not begun shooting..... Read more
6th annual lil ragamuffin summer camp fundraising event 7/15
Join the Sunshowah family as we help host the 6th annual Lil Raggamuffin Summer Camp fundraising event this sunday 7/15/12 at brooklyn stoops, located at 748 Myrtle ave off the... Read more
The Preview to the Update
Update coming sometime next week along with long overdue mixtapes! Daoud and I have made a few tweaks to the script tonight in the last few days that we are... Read more
Thank YOU!
We crossed the finish line, exhaled, collected our bearings, and now... we are thanking you all!
$20,000 and Beyond...
Yes, we did it, and we would like to be the first to congratulate...all of you! It was the strength in numbers that got this project funded and closer to... Read more
Making A Difference
When most folks think of Jamaica, it's pretty safe two say that two competing images immediately spring to mind. On the one hand, the beautiful beaches and cool reggae music... Read more
Sunshowah Interview w/ http://www.inhershoesblog.com in his shoes
we discuss some of our motivation, and intentions.. for the full interview follow this link: http://www.inhershoesblog.com/in-his-shoes#comment-6856
Blogs: In His Shoes / ESPN / Largeup.com
Sunshowah directors Daoud Abeid & Dahkil Hausif have been featured on two blogs this week talking about the Kickstarter project:
Inhershoesblog.com: http://www.inhershoesblog.com/in-his-shoes
Largeup.com: http://largeup.okayplayer.com/2011/11/16/the-harder-they-fall-sunshowah-films-makes-its-1st-feature-in-jamaica/
and listen to Dahkil's interview about being... Read more
Sunshowah Blog
If you haven't had a chance to already, check out our blog http://www.sunshowah.com/blog/
for more information about this project and our adventures throughout this Kickstarter process! Thank you for your support!
Featurette - Gabeana Campbell
"...The Harder They Fall" actress, Gabeana Campbell talks about her poetry and acting as well as playing the role "Uminah" in "...The Harder They Fall"
Featurette - Clayton Brown
Meet Clayton Brown as he talks about life in Settlement and his role in "...The Harder They Fall"
Featurette - Tamara Gabas
"…The Harder They Fall" actress Tamara Gabas talks about her inspiration, being a poet and becoming a star!
Visit www.sunshowah.com for pictures of our location, plus stay posted with more... Read more
1st update.. we have reached $40% of our $20,000 goal
Good Day Sunshowah friends.. We at Sunshowah are so pleased and excited to share that as of our launch Friday 10/28 with your support we have already reached 40%of... Read more


text:
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Healthy Options P1000 Gift Card
Healthy Options is Asia's # 1 Natural Product Store Chain. At Healthy Options, we simplify your gift giving experience. Whether you are selecting a gift to show affection, appreciation, or recognition . . . to express joy, caring or esteem . . . you want the recipient to know you took personal care in selecting their special gift.
Use of this card constitutes acceptance of the following terms and conditions:
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Appliances & Oversize Items US$ 45.00 / 10kg max US$ 90.00 /40kg max (Add US$ 1.60 per additional kg) 6-7 days delivery
2016 eBuy Philippines Online Shopping in the Philippines


text:
Contest: Worst Tech Gift
While not everyone can get gifts as awesome as my 512MB High School Musical MP3 player, we all have thoughtful relatives that try to get us geeky gifts. Sometimes they do well (especially if they stick to the Amazon Wish Lists we create), but sometimes they do poorly. I'll leave it up to you to decide which way my Mix-Stick leans.
In true holiday spirit, we figured misery loves company. We want to see your worst tech-related gift this year. Perhaps you got an 8-Track player. Maybe a battery powered pasta sorter. We all get horrible tech gifts this time of year. Heck, maybe someone gave you a Windows 7 phone. Whatever your worst tech gift is this season, we want to see it.
Attach a link to a photo or video of your tackiest, most pointless, completely age inappropriate gift in the comment section. The Linux Journal staff will pick the best of the worst, and send that person a special gift pack filled with stuff around the office. You'll probably get stickers, a t-shirt, old tea bags, partially consumed mouse pads, you know -- the stuff you'd find in any geeky magazine office. :)
You have all week to submit your photos and videos, we'll be picking a winner next year. (I love those "next year" jokes this time of year, heheheh) So polish up that hand-crank-generator-camera, and take some photos or videos of your aw{ful|some} gifts in action.
(Oh, and yes -- the MP3 player really is mine.)
Shawn Powers is a Linux Journal Associate Editor. You might find him on IRC, Twitter, or training IT pros at CBT Nuggets.
Photo display key-ring
Photo display key-ring from... the sharper image.
I know, the store still exists? I was surprised too.
I don't have a picture since I didn't bring it back from my folks house, but here's a link to the same item on Amazon:
I respect the thought, and know it's hard to find tech gifts for tech people, but... really? It's a ton of bulk for something completely useless.
Regifted Goodness
Ok, I layed it on heavy this year. I sent my wife several links to precisely what I wanted. I kept the gifts small and laced the "hints" with the exact reasoning behind the gift. They were all little time saving gadgets and gizmos that, while I wouldn't shell out the cash for them myself, would save me time and help my home network become more something I could be proud of in a technical sense and less of a Red Green sense.
So, with all these hints what do I get?
Yeah. I literally got that. As an aside, the bag was filled with a late 80's MonteCarlo SS MatchBox(r)... but c'mon!!! Note even one techie gift.
Now, I'm not a lemons to lemonade type of a guy, but desperate times call for desperate measures. I happened to notice that my Aunt got a really cool new 802.11N router... they're SO MUCH FASTER for browsing the Internet you know.
On a hunch I offered to setup her new router and responsibly recycle the old one. Care to guess what's running DD-WRT and will soon be used to bring Internet radio to my back yard shed?
Merry Christmas, to ME!
This would be a losing entry.
Got some CyberClean goop for cleaning my keyboard. Closest "tech" gift I received this year. I actually find the product pretty good. :-)
8-tracks FTW!
I didn't really get any tech gifts this year (unless you count the Back and Decker Electric Screwdriver). I just wanted to say that just four years ago I got an 8-track player for Christmas. The sad thing is that I actually wanted it, and that I listen to 8-track tapes (even though they had fizzled out of popularity before I was born).
You mean people actually do give tech gifts? I ask, but never receive.
Does this count?
I'm not entirely sure this counts, but my step-mom, who knows I'm a huge Trekkie, gave me "Star Trek: First Contact" and "Star Trek: Insurrection"... on VHS.
Laugh at life or life will laugh at you.
But, pics or it didn't happen. :D
Shawn Powers is a Linux Journal Associate Editor. You might find him on IRC, Twitter, or training IT pros at CBT Nuggets.
I couldn't figure out how to embed the pic, so you'll have to click on the link.
I should add that got both movies on DVD several years ago (I haven't gotten into Blu-Ray yet).
Laugh at life or life will laugh at you.
Not a problem
I can grab a pic of the videos this evening when I get home.
To be fair, the front cover of "First Contact" video is somewhat cool (as far as video covers go, anyway). It has a lenticular image (meaning the image changes slightly depending on what angle you are looking at it). So if nothing else, I'll probably keep that cover.
Laugh at life or life will laugh at you.
I had that same VHS tape. I had forgotten about the cool cover thingy. Also, you taught me a new word. ;)
Shawn Powers is a Linux Journal Associate Editor. You might find him on IRC, Twitter, or training IT pros at CBT Nuggets.
Awesome. I also just learned the word "lenticular" last night, when I bought my daughter a "lenticular" Toy Story 3 puzzle. It's a hard puzzle!
Nothing wrong with a mix-stick...
My daughter actually has a mix-stick and it served its purpose. OK, I can see how you might not actually dig it Shawn (I always thought you were more of a Hannah Montana fan myself), but for a "first" MP3 player for the under 10 set, they are pretty well designed, take upgrade chips (some) and teach the little ones responsibility. If they are really good, they get upgraded to real players.
I am sure I have had some bad tech gifts. But you know, I have a hard time remembering what they are...maybe I need something to help me track my bad gifts. Of course, since I am a Luddite, and most of my friends know I am a Luddite, I generally do not get tech gifts.
But I can tell you I did get one awesome tech gift this year. A wireless trackball! (The rest of my gifts were also awesome, just less technical :) )
David Lane, KG4GIY is a member of Linux Journal's Editorial Advisory Panel and the Control Op for Linux Journal's Virtual Ham Shack
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With all the industry talk about the benefits of Linux on Power and all the performance advantages offered by its open architecture, you may be considering a move in that direction. If you are thinking about analytics, big data and cloud computing, you would be right to evaluate Power. The idea of using commodity x86 hardware and replacing it every three years is an outdated cost model. It doesn't consider the total cost of ownership, and it doesn't consider the advantage of real processing power, high-availability and multithreading like a demon.
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text:
It is already October (at least it will be come Saturday). That means many teams' seasons are a third of the way done.
We've labeled the contenders (LSU, OU, Alabama) and the pretenders (FSU, Notre Dame, Georgia), but what about the teams that are playing really good football and flying under the radar?
There are two qualifications to be on this list. One, you must be unranked as of today. And two, you have to have a legitimate shot at getting to the BCS.
Apologies to a team like Mississippi State, who may be as good as the teams listed, but have a slim-to-zero percent chance of making it to a BCS game.
Remember, all these teams are unranked, which makes them all long shots. This isn't a list of teams that are definitely going to make the BCS, just underrated ones who provide a threat.


text:
Skip to comments.AMERICANS OWN LARGEST NUMBER OF FIREARMS IN WORLD
Posted on 07/08/2003 10:42:28 AM PDT by NativeNewYorker
click here to read article
|A Recall AND a Fundraiser? I'm toast.
|Let's get this over with FAST. Please contribute!
And, to the UN ...
Don't you forget it!
BTW, I don't see anyone cracking on Finland for their relatively high precentage of gunowners.
Someone please explain this. Convient too that they left out UNREGISTERED guns. The #'s would be alot closer if they included the armories of the criminal element.
So, they want to ban something that is already banned?
And in a related story
I have heard that there is an average of 2 per adult down here. We have 21 million citizens, and you have to figure that 2/3 or 14 million are adults...28 million! We're well prepared to defend the Alamo this time.
And in a related story ... AMERICA CELEBRATES 227 YEARS OF FREEDOM
This can't be repeated often enough.
Well, my wife and I are doing our part ... we've exceeded that average by 100%: small concealable pistol, heavier pistol, shotgun, and rifle each ...
Sounds a little low ... at least in my household.
I know people who have more than that.
You betcha. Six months ago, the day I moved from New York to NC, I bought a Browning .380 and a Taurus .17HMR.
Don't forget the bullets!
No good coming home to an empty gun! < /monty python screeching british accent >
I live here too
Heard from Him lately?
Where are You at?
Yup. 4 pistols, 2 revolvers, 4 rifles, and 6 shotguns are in my inventory. I also reload all calibers and gages except a .22 and .17 HMR. Messers Browning, Charles Daly, Colt, Smith, and Wesson are my friends.
Why didn't the report mention that progress has been particularly slow in the United States? Has our Government signed up with the UN to disarm the people of the United States? Our politicians seem to sign up for whatever the UN wants. I would have to assume that Schumer and Feinstein have signed our country up, but are trying to keep it a secret.
I think you mean "cartridges."
Might as well try to ban the wind.
Such weapons are easily manufactured- there are villages in Pakistan that can create a well-made duplicate of almost any weapon you bring them, without the use of modern machine tools.
There are an estimated 600 million such weapons around the world, both legally and illegally owned.
Illegally according to whom?
What they especially don't want to own up to is the fact that virtually all of these deaths they're talking about are caused by governments and those acting as agents of governments, and not by plain old civilians.
Disclaimer: Opinions posted on Free Republic are those of the individual posters and do not necessarily represent the opinion of Free Republic or its management. All materials posted herein are protected by copyright law and the exemption for fair use of copyrighted works.


text:
Music to Your Ears
By Laura Marrich
Reggae Under the Stars ... with Nachos—Local reggae collective One Foundation/Mystic Vision is taking their message-heavy music to an unlikely venue this weekend: El Pinto (10500 Fourth Street). It's the first I've heard of a traditional New Mexican restaurant hosting anything but mariachis, but why not? It's got that beautiful North Valley patio, which Mystic Vision says they'll happily take advantage of. The group will be filming the performance for a DVD they're putting together. Show starts around 7:30 p.m. on Sunday, June 25. I'm not aware of any cover charge, but you should probably order something to make the owners happy.
Wes Naman
Show Up!
SuperGiant CD Release Party
Headphone fanatics rejoice
By Simon McCormack
"Listen to it with headphones," advised guitarist Jeremy McCollum as he handed me a copy of his band's new CD. "OK," I said, smiling meekly, unsure quite what to make of what he had just told me.
And Then There Was One
He's the last man standing—and that's fine by him
By Marisa Demarco
It used to be a plural affair, The Slow Poisoners. Over the course of 10 years, the other four members "expired." That's how Andrew Goldfarb, the remaining Poisoner, puts it. "Eventually, it was down to a duo," he says. "When I saw an opportunity to off the other member, I took it."
Flyer on the Wall
Giant Robot
SuperGiant is a lean, mean rocking machine. See this week's "Show Up!" for details. (LM)
Music Magnified
Absolut House Six-Year Anniversary featuring Franck Roger
with DJ Eldon
By Marisa Demarco
Thursday, June 22, Martini Grille (21-and-over); $5: His United States tour stops include Chicago, Los Angeles, San Francisco, New York, Dallas—and Albuquerque.
Music Playlist
Some Handsome Music
Brett Sparks of The Handsome Family reveals the tunes at the top of his iPod playlist
1. "Dear Prudence," Brad Mehldau, Largo
Sonic ReducerPeople Under the Stairs Stepfather · Elan Together as One · John Ralston Needle Bed
By Simon McCormack
The long-awaited full-length release from this Los Angeles hip-hop duo is arguably the tandem's most meticulous and spirited disc to date. The benign subject matter that makes up PUTS' flows (songs about food and hitting on girls at barbecues are the norm) are matched by the similarly low-key, self-produced beats. It won't get the party started, but it will give you a nice soundtrack to wind down to after the keg is cashed.
EVENT HORIZON (Friday, Sep 30)
'A Lot About Living'
Alan Jackson • country
By Joshua Lee
As Ludwig von Beethoven once said, "Music is the mediator between the spiritual and the sensual life." Those words still carry weight, even now: a thousand years later. And you'll find no better example than the incomparable Alan Jackson, whose voice and countenance rival those of the gods, as though he were hewn from the heart of the sun itself. His honky tonk tunes are swords of righteous terror and beauty which melt the eardrums…
Landon Speers
EVENT HORIZON (Friday, Sep 30)
Desert Sound
Chrome Sparks • electronic, indie pop
By Joshua Lee
So Chrome Sparks apparently has a few hours open in his schedule to visit ABQ—somewhere in between juggling his gajillion other projects and fulfilling his role as busiest spacey electro-pop composer on the planet. New Mexico seems like the perfect place for those wide-open, expansive tunes of his…
EVENT HORIZON (Monday, Oct 3)
Two i's Are Better Than One
Miike Snow • indie, electro-pop
By Monica Schmitt
"I change shapes just to hide in this place, but I'm sure not going to hide from this concert." Ladies and gents, I am happy to report that Miike Snow, the Swedish electro-pop band of your dreams, will be performing at our very own Historic El Rey Theater. Imagine Alt-J and Dan Black created a musically inclined love child…
EVENT HORIZON (Wednesday, Oct 5)
Tome-ate-o, Tom-ato
Andrew Jackson Jihad • folk-punk • Diners • surf, indie rock • Kepi Ghoulie • punk folk
By Peter Karlsen
Once upon a time, back in 2004, in the distant land of Arizona, there was born a folk-punk band by the name of Andrew Jackson Jihad. It was a mouthful, so they decided to go by the acronym AJJ. Then they decided to formally change their name to that. Soon they'll be at the Launchpad. People in this town like to shit on the folk-punkers, but fuck those jerks…
Courtesy of the Palisades Facebook Page
EVENT HORIZON (Sunday, Oct 9)
Hit 'em with the High and the Low
Palisades • electronicore • It Lives, It Breathes • post-hardcore • Darke Complex • metal
By Megan Reneau
There is a rhythmic connection between genres dubstep and metalcore. One band that's done phenomenal in this kind of mashup (referred to as electronicore) is Palisades originating from Iselin, N.J. Having been to their last show in town, I can guarantee that they put on an equally energetic and high quality show, which makes sense because after going on over 20 tours in the last four years, they are likely to have nearly perfected their performance technique. These guys just don't quit—or take a break for that matter—and I'm glad they haven't. In addition to the supporting bands It Lives, It Breaths and Darke Complex this is going to be a good fucking time. They'll be in town on Sunday, Oct. 9, at the gem of a locale, Blu Phoenix Venue,and tickets are only $8! Doors open at 6pm, and it's likely to be packed so get there on time (or early).
NEWSLETTERS Great Alibi stories, events and deals delivered to your inbox each week. No fooling!
Today's Events
Chrome Sparks • electronic, indie pop at Sister
Alan Jackson • country at Sandia Resort & Casino
Hanta • stoner rock • Econarchy • metal • Black Maria • rock • Nite Kids at LaunchpadMore Recommended Events ››
Hero Quest • 10.7.2016
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(based on 2 reviews)
Ratings Distribution
5 Stars
4 Stars
3 Stars
2 Stars
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Reviewed by 2 customers
Displaying reviews 1-2
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Stylish alternative
By mcamus
from Stamford, CT
I use the wooden discs for my work notebook -- they travel with me every day and get fairly heavy use. So far I really like them -- I find that the pages slide around the discs just fine, especially the pages with the newer, slightly bigger Circa holes.
Bottom Line Yes, I would recommend this to a friend
Was this review helpful? Yes / No
- You may also flag this review
Stylish but Brittle.
By Nathan
from Undisclosed
I've had these in 1" for about a month. They look very nice but function poorly. My first complaint is that the paper doesn't slide very well with them. If I open my notebook half way and lay it flat and then try to close it in one motion the paper doesn't slide into position thus binding up the covers. Either the disc are bit too thick or the finish isn't slick enough for the paper to slide without catching and binding. It's more of a nuisance than anything as you have to move small amounts of paper at a time if you plan on flipping through the notebook. The second and final complaint was I broke a disc while the notebook was in my backpack. It just snapped into two pieces. At this point I decided to move back to the black stock disc and when removing the remaining wood disc I snapped a second disc. Overall I'm not impressed with the performance what so ever. They looked great though….
Bottom Line No, I would not recommend this to a friend
Merchant response: Thank you for the detailed review of the Circa Wooden Discs. We apologize for the inconvenience this has caused for you. We have also submitted your comments to our Product Development Team for further review and will be contacting you to discuss ways to make this right.
© Copyright Levenger Company
2016. All Rights Reserved.


text:
Wladimir Klitschko A Fan of Borat
By Mark Vester
IBF heavyweight champion Wladimir Klitschko, who was born in Kazakhstan, and grew up in Ukraine, gave his comments on the movie "Borat," a comedy based on a journalist from Kazakhstan that travels to America to learn about the culture.
The movie Borat is getting a lot of negative feedback from the people of Kazakhstan, because many of them feel that the movie, and actor Sacha Cohen who plays Borat, portrays them in bad light by making them out to be perverts, being unintelligent, and stating that they hate Jewish people.
Despite all of the bad press, Wladimir Klitschko loves the actor and is a very big fan of the movie. He thinks Borat will do for his country what Crocodile Dundee did for Australia.
"I love 'Borat'. I think that is the funniest dude I have ever seen," Klitschko said. "He's just something different than we have seen before. I think it might be great for Kazakhstan because people will go to the country and see it. You remember that crocodile hunter from Australia? Everybody thought that when he comes to New York in the movie all Australians are as crazy as Crocodile Dundee. Many people went to Australia to see that country and it was good for the economy."
[QUOTE=Kid Achilles;1940587]I highly doubt it was a joke. The actual article discussed Wladimir's charity efforts in some depth and seems like a legitimate interview.[/QUOTE] if so, then why doesn't MTimes give credit to BS for the interview... or, why doesn't…Comment by Kid Achilles on 11-23-2006
I highly doubt it was a joke. The actual article discussed Wladimir's charity efforts in some depth and seems like a legitimate interview.Comment by Nautilus on 11-23-2006
[QUOTE=Kid Achilles;1940051]BPP didn't make it up. Google Wladimir Klitschko and you'll see the article for the Moscow times.[/QUOTE] Moscow times gets their news from boxingscene's lounge and somethime from NSB. :)Comment by jack_the_rippuh on 11-23-2006
[QUOTE=Leo Pradun;1940023]that picture explains everything....[/QUOTE] Shut your fat ass diabetic face up.Comment by Kid Achilles on 11-23-2006
BPP didn't make it up. Google Wladimir Klitschko and you'll see the article for the Moscow times.Post a Comment - View More User Comments (30)


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The Homeschool Minute™ is a weekly E-Newsletter for anyone homeschooling or considering starting. It's brief yet packed with a powerful dose of encouragement to keep you going strong in your commitment to your family and to the Lord.
Read the Current Issue of The Homeschool Minute!
Click the image below
If you're a program director and would like to air these episodes on your station, please contact Gena Suarez at


text:
Two second-half goals by Emily Dixon gave Broome (N.Y.) Community College a 2-1 victory over Gloucester County (N.J.) College in the NJCAA Division III Women's final on Sunday in Herkimer, N.Y.
For the top-ranked Hornets, it was their first National Junior College Athletic Association Division III title in any sport.
Broome had entered the national finals with a 111-6 scoring margin and had not trailed since the third game of the season, but it fell behind in both games at the final four.
After trailing early on a own goal, the Hornets crushed host Herkimer, 7-2, thanks to a four-goal barrage in a 22-minute period.
Texas powerhouse Richland's took third with a shootout victory over Howard (Md.) Community College in the consolation game.
Broome-Gloucester report from Binghamton Press & Sun-Bulletin
Broome-Gloucester report from Salem Sunbeam
Broome-Howard report from Binghamton Press & Sun-Bulletin


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MySQL and MATLAB connectivity
This project was successfully completed by ashafaei for $30 AUD in 3 days.Get free quotes for a project like this
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Project Budget$30 - $250 AUD
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Project Description
I want to connect my MATLAB 2013a (x64) with my current database using MySQL. I have the Datafeed/Database toolboxes. I would like to do this through JDBC using MySQL (not MS SQL server).
I have been unsuccessful in retrieving data from MATLAB that sits on my database.
I would like to use TeamView to do this project.
I will only consider hiring people who have done this before, thanks.
Please note as this is a very quick job for those who know what they are doing, the highest bid I am willing to accept is $50.
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Behringer Pro Audio
Results 1 - 40 of 249 matches
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Hilleberg Akto Footprint
Where to Buy
sponsored links
Retailers' Descriptions
Here's what other sites are saying:
Free Shipping. Hilleberg Akto / Enan Footprint FEATURES of the Hilleberg Akto / Enan Footprint Tough ground sheet that protects the tent's floor from rips, dirt, and ground moisture from rising in the vestibule area This footprint is part of Hilleberg's Red Label, which means it covers the full area of the outer tent
- Moosejaw
Optional footprint for Akto protects wear on the tent's floor and aids against ground moisture rising into the vestibule. The footprint covers the entire area of the outer tent, including the vestibule, connects directly to the tent via toggles, and can be left attached during pitching. Weight: 9oz (weight includes stuff sack).
- Bentgate.com
Hilleberg Akto Footprint
currently retails for:


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Your Account | Your Basket | Checkout
All brands and names are the property of their respective owners.
Original Cartier folding clasp, width 18 mm, stainless steel, silvertone, matt, with Cartier engraving.
Fits Roadster, Pasha and others.
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Changes in prices and errors excepted.


text:
Borderlands 2
Thank you very much my friend.
Had an immediate freeze with Trainer; unsure with Editor.
look him in the eye, nod politely, and KILL THE LIVING CRAP OUT OF HIM!" - Tiny Tina
Editor is crashing the game, have'nt tried trainer but as poster before me said it freezes the game.
hope we will get trainer/editor soon(ish) if devs have the time.
The updated Trainer worked [for the new BL2 update today], but my inventory slots had been reset from 1,000 to 27. I didn't lose any of my inventory (I hope!), but I could not pick up any new weapons or items
I'm hoping the updated Editor can fix that.
[Edited by drmaxman, 11/13/2012 8:07:55 PM]
[Edited by drmaxman, 11/13/2012 8:16:13 PM]
look him in the eye, nod politely, and KILL THE LIVING CRAP OUT OF HIM!" - Tiny Tina
Also, I was curious if it would be possible to request a feature. The ability to level or delevel an item would be amazing - I know that there are utilities to do that over at Cheat engine, but a CH version that is updated on a regular basis would be quite useful, and very appreciated.
• Unlimited Health
• Armor
+ 16 more options
• 1152 x 600
• 1280 x 960
• 1280 x 1024
• 1600 x 1200
• 1920 x 1200


text:
Newegg.com - A great place to buy computers, computer parts, electronics, software, accessories, and DVDs online. With great prices, fast shipping, and top-rated customer service - once you know, you Newegg.
If you are reading this message, Please click this link to reload this page.(Do not use your browser's "Refresh" button). Please email us if you're running the latest version of your browser and you still see this message.
Newegg.com - Computer Parts, Laptops, Electronics, HDTVs, Digital Cameras and More!
Showing Results: Most Recent
This review is from: Samsung SCX-4729FW MFC / All-In-One Up to 29 ppm 1200 x 1200 dpi Color Print Quality Monochrome Wireless 802.11b/g/n Laser Printer
Pros: Easy to set up. Fast print time. Feeder works very well. Text is very clear.
Cons: I bought this all in one printer so that I can make double sided copies. When I looked up the features it stated Duplex printing. This is true, but it's not a Duplex copier which my wife needed for her work. Knowing what I know now, I would have been better off buying the cheaper model.
Other Thoughts: When I called Samsung customer service, the rep told me that I should be able to make double sided copies. He did state that he's just taken the info off the website. Although he was pleasent, he didn't know the product.READ FULL REVIEW


text:
Indoor Open Car Show
November 11, 2016 in Branson, Missouri
Branson Convention Center on the Branson Landing
Also enjoy the festivities of Branson's annual Veterans' Week Celebration!
Volunteers4Warriors is a 501c3 charitable organization that exists to raise money for various charities. Operating with an all-volunteer staff, 95¢ of each dollar raised goes toward supporting:
Green Beret Foundation—provides immediate and long-term support to the Green Beret community, including: the wounded, their families and the fallen.
METAvivor Metastatic Breast Cancer Awareness, Research and Support—whether serving in the military or supporting a loved one, women play a vital role in our nation's Armed Forces. Because approximately 1 in 8 women will be affected by breast cancer, it is important to support MBC research, which provides hope and helps improve the longevity and quality of life for MBC patients.
No Greater Sacrifice—honors the sacrifice of fallen service members by providing scholarships for their children.
Why we proudly support METAvivor.
May, 25, 2016
Many wonder why a volunteer organization called Volunteers4Warriors would pick METAvivor as one of its charities. To us it's simple: we value women and their role in our Armed Forces, and we hate cancer. Please continue reading about METAvivor and the work this organization does to help improve the longevity and quality of life for […]
We've Moved Indoors.
May, 25, 2016
We are very excited to bring the Volunteers4Warriors annual car show Indoors to the Branson Convention Center on the Branson Landing. We're also thrilled to be in Branson during the annual Veterans' Week Celebration. What a great time for Volunteers4Warriors—and in November, what a great time to be inside! Here's a just a sampling of […]
Contact Us
P.O Box 481
Omaha, AR 72662


text:
Translation Project - hero62 (1)
This project was successfully completed by hero62 for $226 USD in 10 days.Get free quotes for a project like this
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Project Budget$226 USD
Completed In10 days
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Exclusive translation project and payment to user.
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- Forbes
- The New York Times
- Time
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Online Since 2006
Page Views
"Don't stay in bed, unless you can make money in bed." George Burns
When you have your website up and running, and your mailing list, a really fun thing to do is to have a competition. It's easy, it's a bit of fun, it's newsworthy and it gives you the opportunity to engage with your visitors/subscribers in a different way for a change. There are also other benefits.
JVs or Joint Ventures are THE way forward to success in business. But how do you get started with JVs? How can someone who doesn't know ANYONE yet break into the secret circles of JV marketing and learn to make these valuable connections ANYWHERE?
On discovering that I was going to play an experiment in Internet Publishing and Marketing, Martin Curran wrote on the MM List: "Anyway, I don't doubt you'll learn a lot from your new marketing experiment, you're a gutsy lady, I just worry for your immortal soul :) !"
Is marketing evil? Read my response ...
Once a cherished project such as your first ebook or ecourse has been completed, we need to turn our attention to the marketing of your project. Here's where things sometimes turn somewhat difficult if the author and the marketeer are one and the same person, and here's where we have to be cautious and take the right steps in the right order, so we might stay on track. Here is the MOST IMPORTANT and FIRST STEP you need to take - before you even start marketing!
Silvia Hartmann writes: I'm currently assembling a hypnotherapy directory and this involves looking at a great many websites in rapid progression. There are a number of basic mistakes, or should I say omissions, that people make over and over again, so much so that we must say to ourselves, "This is a structure, this is natural to people who are doing their own marketing to be doing this."
Module Menu
Modern Meditation with Silvia Hartmann: Powerful Stress Relief & Mind Control Made Easy by Silvia Hartmann It's scientifically proven - meditating is good for you! The StressFish Guide to Modern Meditation has been designed to be the perfect, stress-free introduction to meditation for the 21st Century.


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Elton John's Husband Trashes Madonna For Golden Globe Win And Speech!
Madonna had just won Best Song for co-writing something that pops up in the February release W.E.
Sir Elton (who's British royalty just like Dame Madge herself) hadn't won for "Hello Hello" from the animated movie about lawn gnomes in love.
And Sir Elton couldn't disguise his apparent indigestion as the camera closed in on him.
He looked like he was smelling some kind of "Masterpiece" all right, especially since he'd told a red-carpet interviewer that Madge didn't have "a fucking chance" to win.
Upcoming Events
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TicketsMon., Sep. 26, 7:30pm
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Elton's consolation? His song is on the qualifying list for an Oscar nomination. Madonna's is not.
UPDATE: Elton's husband, David Furnish, went even further. Here's the message on David's Facebook page: "These awards have nothing to do with merit. Her acceptance speech was embarrassing in its narcissism. And her criticism of Lady Gaga shows how desperate she really is."
Ooh! British snit fight!
Meanwhile, strong reactions continued when Madonna used her other Golden Globes screen time -- when she presented Best Foreign Film -- to gush about her early love of films by foreign auteurs like Luchino Visconti, art-house gems that must have filled the screens of Bay City when she was a child.
Not since Streisand compared herself to Kurosawa on the very same awards telecast have I been so visibly moved.
But the best reaction shot of all came courtesy of the fat guy from Modern Family when Matt LeBlanc won.
Here it is, at the 1:29 mark. It's worthy of Visconti.
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Show Feed
Paul Pabst @PaulPabstRT @NBCSports: 18 games in the @NFL? It just doesn't seem to make sense to the NFLPA. https://t.co/Boeyk49UxP https://t.co/miB1N9Ywmn4 hours ago
Fan Feed
J@dpshow What a week! Vin, UMilk, McKnight, Mark, Usain, Frank. Nice job @brooklynfritzy!3 hours ago
Jared Myracle@PaulPabst @dpshow Can I nominate this for "Homer call of the week" even if I really enjoyed it? https://t.co/jMeeCXDPwR4 hours ago
FrankRT @andrewperloff: Dan asked Jim Harbaugh if he could start a team with one QB. "Andrew Luck" Harbaugh said immediately. @dpshow4 hours ago
KnoxThis is out of the blue but when is @foxsports910 going to stop playing @dpshow with an hour delay?4 hours ago


text: r Delane's Mom | Digital Scrapbooking at Scrapbook Flair Home Galleries Groups Challenges Blogs Forums Members Downloads MarketPlace Store Archive Join Login offline Joined: Dec 11, 2007 Last Logon: Sep 14, 2011 Delane's Mom's Home Page www.scrapbookflair.com/Delanes_Mom I am 32 and happily married to a stunning blond. I have to gorgeous kids. Delané 5 and Dwaine 3. I have been living in Middelburg for 14 years. I am new at digital scrapping. I started 7 Desember 2007 and I must confess, I am hooked. I just love to scrap. I can't wait to learn more from the pro's Delane's Mom is from Middelburg, Mpumalanga, South Africa 1 Delane's Mom's Scrapbooks God can move mountains Walk like an Egyptian This is who I am email it » view all Delane's Mom's scrapbooks Delane's Mom's Favorite Scrapbooks » view all Delane's Mom's favorite scrapbooks Delane's Mom's Friends Delane's Mom has 40 friends » view all Delane's Mom's friends Delane's Mom's Fans Delane's Mom has 33 fans » view all Delane's Mom's fans Renew/Upgrade Membership Gallery Award Gallery Groups Blog Friends Favorites Tags Post and Share this page on Blogs and other Websites by copying and pasting this link: http://www.scrapbookflair.com/Delanes_Mom Delane's Mom's home page has been visited 1403 times. About Us Bookmark Contact Us Designer Members FAQs Fan Mail Help Link to Us Membership News Privacy Search Site Rules Suggestion Box Tell a Friend © 2006-2016, Patent Pending, Aurora Digital Imaging, Ltd. Beta 5.0 The most fun digital scrapbooking site on the Internet. Free digital scrapbooking software, scrapbook templates, scrapbook embellishments and backgrounds. Beautiful scrapbook prints and photobooks.


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GENESEE COUNTY, MI – Local health agencies are working to make some major changes throughout the county to promote healthy living but still have a ways to go, they admit.
Genesee County ranked the sixth unhealthiest county in Michigan, according to county-by-county rankings released nationally this week.
Ranking 77th out of 82 counties – one county had no data – Genesee County made no improvement in overall ranking compared to last year, which was not surprising to Mark Valacak, Genesee Health Department health officer.
But the county is making strides in some breakout areas, he said.
"Given the economy and the economic conditions in the community, it doesn't surprise me that we're not seeing change in a positive direction. … (The Health Department and other organizations) are trying to address some of these health behaviors that cause us to rank low," Valacak said. "Well this is actually the third year that they've produced these numbers so we've been working to try and improve in a number of areas. We're seeing some progress."
The rankings are a measure of life and death. A county's overall health is based equally on mortality data - how long people live – and how healthy they feel. The latter includes low birth weight information and surveys on physically and mentally unhealthy days per month.
A separate county ranking of "health factors" that influence one's overall health measures 13 variables, including alcohol use, diet and exercise, education, and environmental quality.
In the category of health behaviors for last year's data, Genesee County ranked 82 out of 82 but ranked 77 this year. Health behaviors include things like adult smoking, adult obesity, physical inactivity, excessive drinking, teenage birth rate, motor vehicle crash death rate and sexual transmitted infections.
Although, health behaviors isn't the only area of focus for the overall study, local health care officials said it's the best one to focus on.
Health organizations, such as the Greater Flint Health Coalition and the Genesee County Community Mental Health, have already been taking action to improve the health of the county, Valacak said.
'Step in the right direction'
Kirk Smith, president and chief executive officer of the Greater Flint Health Coalition, said focus should be on the lack of physical activity, nutrition and smoking.
"Those are the biggest areas of challenge," Smith said. "That's sort of the key to everything."
According to the data released 23 percent of adults in the county smoke every day or "most days" and have smoked at least 100 cigarettes in their lifetime. Last year's data showed that at 25 percent.
In Genesee County adults 20 and older, the study found 36 percent of residents to be obese, up from 35 percent last year. And 30 percent of county residents reported having no leisure time physical activity.
The Health Coalition began a county health ranking action plan after the first rankings came out three years ago. Out of that came Commit to Fit, a free program that teaches and challenges residents with nutrition and exercise.
"We've got a long way to go, but it's a step in the right direction," Smith said of the county initiatives that helped move the health behaviors ranking from 82 to 77. "We have health challenges, absolutely. I think the biggest area for us to improve is health behaviors. Physical environment, social and economic factors are key, too.
"It's hard to be physically active when in a violent community. We need to continue work with that on a community level."
Genesee County Community Mental Health has also been working on some initiatives to help increase the county's ranking, said Dan Russell, chief executive officer.
Community Mental Health has continued alcohol and drug preventions in schools, as well as resources for adults who face those issues. More recently Community Mental Health has added an emphasis on nutrition and exercise with its staff and clients.
They started a program called InShape, which brings nutritional counseling and personal training to individuals with mental illnesses. And in the Community Mental Health building, all the stairwells were repainted and carpeted and better lighting and music was added to encourage people to use the stairs more, Russell said. Many employees now use the stairs for exercise during lunch.
"(The initiatives) are starting to make a difference, but (unhealthy living) is a huge problem. It's going to take some time to make those numbers go down at once," Russell said. "I think everyone is aware of (the need to be healthy), which is very different than five years ago. … I think it has at least reached the consciousness of everybody."
Russell said with all the opportunities to get educated about health and the free opportunities to get healthy, not being able to afford it isn't an option any more.
"People just need to take more advantage of it," he said.
Some residents agreed with that.
Rachel Karyakose, 19, of Goodrich, said she doesn't think too many people in the area care about their health.
"I could believe (the low ranking)," said Karyakose, a University of Michigan-Flint student who lives in the Riverfront dorms in downtown Flint. "I see people walk down the street smoking or with a bottle of beer in their hands at 11 in the morning."
She said she tries to eat healthy and workout when she can, but she believes part of the solution to unhealthy living should start with the children and then extend to their parents. Schools are doing a good job with bringing healthy meals and exercise into the daily routine, now the hard part is encouraging parents to do the same, Karyakose said.
"It's learned," she said, adding that there needs to a bigger emphasis on walkable communities in the county. "I think our area is a little unmotivated."
Michael Burnett, 39, of Mt. Morris Township said healthy living comes down to the willingness to make a change.
To him, drugs and obesity seem to be big problems in Genesee County. At least when it comes to exercise, he does see people trying to get active and even making sure to take walks during the lunch break.
"If you want to do it, you'll do it. … It takes a lot of motivation, too. There has to be something there to make them want to (make a change)," Burnett said, adding residents should make a goal and go for it. "It's not that hard to do."
For more information on Commit to Fit, visit www.commit-2-fit.org.
For a complete ranking of health factors and where Genesee County falls click here.
Valacak also encouraged residents to take advantage of all the resources available to them. He recommended that residents visit the SPROUT Coalition website, http://www.sproutgc.org/, to learn some healthy tips and resources.


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Latinos Ready To Vote (LRTV) to is an organization dedicated to promote voter Registration among Latino Americans, naturalize Legal Permanent Residents in Latino communities and promote Conservative Principles. We will work to inform Latinos on issues important to them and their community. Moreover, we will inform them about the benefits of civic engagement to ensure that we, as Americans citizens, have an equal access to legal political rights and representation in all branches of state and federal government. We know that the Latino community is becoming a strong voting bloc. Hence, we encourage Latinos to register to vote and learn about the issues salient to the community and America, such as education, jobs, the economy, immigration, and the broken entitlements system (i.e. Social Security, Medicare, and Medicaid) that will hurt future generation of Americans. Therefore, we seek to encourage all Latinos in America to participate in their community and in America, through civic engagement, success in education, successful entrepreneurship, and participate in the political process by voting.
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Rent Essential Molecular Biology 1st edition today, or search our site for other textbooks by Terence A. Brown. Every textbook comes with a 21-day "Any Reason" guarantee. Published by Oxford University Press.
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The Swan Levine House, la più antica Grass Valley Bed and Breakfast, ha una storia unica come un ospedale locale trasformato centro d'arte. Il più grande dei Bed & Breakfast di Grass Valley, questo edificio vittoriano Queen Anne si siede indietro su una collina che domina la città attraverso i suoi grandi alberi di Magnolia. Il Bed and Breakfast dispone di quattro camere ampie con bagno privato, salotti e un sacco di locali pubblici con una grande biblioteca e la collezione d'arte. Controllare il numero di agosto 2008 Old House ufficiale di quattro pagine sul Swan Levine House!
4,6 su luglio 28, 2010
I am an Artist....staying at the Swan Levine was a real sensory experience for me! I have stayed here several times and I always find something new to see that I absolutely love. The home is GIANT....the garden is a tangly mass of color! Filled with light,cats,artwork and a real sense of warmth...this is a must stay experience if you appreciate art at all. Howard and Peggy are really amazing people who are involved with their community and it shows. The food was perfect...we are not big eaters...Peggy was gracious enough to provide us with fruit,coffee and a little banana bread..just enough to sate you without making you feel like you just ate a brick! The views from the rooms are really great and you are close enough to walk into town for dinner,shows,shopping....I love it and we will always choose to stay here-
3,4 su ottobre 29, 2008
Returning for yet another stay at this Inn is like returning to a place as comfortable as your own home, but a lot more interesting. It's just like going to good friend's home to catch up with them since the last visit.
served each morning at our dining room table
Local eggs, fruits when in season
There are many special house breakfasts created for your stay or over the forty years of our establishment