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Most Cited Journal of Magnetic Resonance Articles
The most cited articles published since 2011, extracted from Scopus.
Ultra-fast MRI of the human brain with simultaneous multi-slice imagingDavid A. Feinberg | Kawin Setsompop
Volume 229, Issue , April 2013, Pages 90-100
The recent advancement of simultaneous multi-slice imaging using multiband excitation has dramatically reduced the scan time of the brain. The evolution of this parallel imaging technique began over a decade ago and through recent sequence improvements has reduced the acquisition time of multi-slice EPI by over ten fold. This technique has recently become extremely useful for (i) functional MRI studies improving the statistical definition of neuronal networks, and (ii) diffusion based fiber tractography to visualize structural connections in the human brain. Several applications and evaluations are underway which show promise for this family of fast imaging sequences. © 2012 Elsevier Inc. All rights reserved.
Spinach - A software library for simulation of spin dynamics in large spin systemsH. J. Hogben | M. Krzystyniak | G. T P Charnock | P. J. Hore | Ilya Kuprov
Volume 208, Issue 2, February 2011, Pages 179-194
We introduce a software library incorporating our recent research into efficient simulation algorithms for large spin systems. Liouville space simulations (including symmetry, relaxation and chemical kinetics) of most liquid-state NMR experiments on 40+ spin systems can now be performed without effort on a desktop workstation. Much progress has also been made with improving the efficiency of ESR, solid state NMR and Spin Chemistry simulations. Spinach is available for download at http://spindynamics.org. © 2010 Elsevier Inc. All rights reserved.
CEST: From basic principles to applications, challenges and opportunitiesElena Vinogradov | A. Dean Sherry | Robert E. Lenkinski
Volume 229, Issue , April 2013, Pages 155-172
Chemical Exchange Saturation Transfer (CEST) offers a new type of contrast for MRI that is molecule specific. In this approach, a slowly exchanging NMR active nucleus, typically a proton, possessing a chemical shift distinct from water is selectively saturated and the saturated spin is transferred to the bulk water via chemical exchange. Many molecules can act as CEST agents, both naturally occurring endogenous molecules and new types of exogenous agents. A large variety of molecules have been demonstrated as potential agents, including small diamagnetic molecules, complexes of paramagnetic ions, endogenous macromolecules, dendrimers and liposomes. In this review we described the basic principles of the CEST experiment, with emphasis on the similarity to earlier saturation transfer experiments described in the literature. Interest in quantitative CEST has also resulted in the development of new exchange-sensitive detection schemes. Some emerging clinical applications of CEST are described and the challenges and opportunities associated with translation of these methods to the clinical environment are discussed. © 2012 Elsevier Inc. All rights reserved.
Very fast magic angle spinning 1H-14N 2D solid-state NMR: Sub-micro-liter sample data collection in a few minutesYusuke Nishiyama | Yuki Endo | Takahiro Nemoto | Hiroaki Utsumi | Kazuo Yamauchi | Katsuya Hioka | Tetsuo Asakura
Volume 208, Issue 1, January 2011, Pages 44-48
Substantial resolution and sensitivity enhancements of solid-state 1H detected 14N HMQC NMR spectra at very fast MAS rates up to 80 kHz, in a 1 mm MAS rotor, are presented. Very fast MAS enhances the 1H transverse relaxation time and efficiently decouples the 1H-14N interactions, both effects leading to resolution enhancement. The micro-coil contributes to the sensitivity increase via strong 14N rf fields and high sensitivity per unit volume. 1H-14N HMQC 2D spectra of glycine and glycyl-l-alanine at 70 kHz MAS at 11.7 T are observed in a few minutes with a sample volume of 0.8 μL. © 2010 Elsevier Inc. All rights reserved.
Quantitative separation of CEST effect from magnetization transfer and spillover effects by Lorentzian-line-fit analysis of z-spectraMoritz Zaiß | Benjamin Schmitt | Peter Bachert
Volume 211, Issue 2, August 2011, Pages 149-155
Chemical exchange saturation transfer (CEST) processes in aqueous systems are quantified by evaluation of z-spectra, which are obtained by acquisition of the water proton signal after selective RF presaturation at different frequencies. When saturation experiments are performed in vivo, three effects are contributing: CEST, direct water saturation (spillover), and magnetization transfer (MT) mediated by protons bound to macromolecules and bulk water molecules. To analyze the combined saturation a new analytical model is introduced which is based on the weak-saturation-pulse (WSP) approximation. The model combines three single WSP approaches to a general model function. Simulations demonstrated the benefits and constraints of the model, in particular the capability of the model to reproduce the ideal proton transfer rate (PTR) and the conventional MT rate for moderate spillover effects (up to 50% direct saturation at CEST-resonant irradiation). The method offers access to PTR from z-spectra data without further knowledge of the system, but requires precise measurements with dense saturation frequency sampling of z-spectra. PTR is related to physical parameters such as concentration, transfer rates and thereby pH or temperature of tissue, using either exogenous contrast agents (PARACEST, DIACEST) or endogenous agents such as amide protons and -OH protons of small metabolites. © 2011 Elsevier Inc. All rights reserved.
Theoretical aspects of dynamic nuclear polarization in the solid state - The cross effectYonatan Hovav | Akiva Feintuch | Shimon Vega
Volume 214, Issue , January 2012, Pages 29-41
In recent years Dynamic Nuclear Polarization (DNP) signal enhancement techniques have become an important and integral part of modern NMR and MRI spectroscopy. The DNP mechanisms transferring polarization from unpaired electrons to the nuclei in the sample is accomplished by microwave (MW) irradiation. For solid samples a distinction is made between three main enhancement processes: Solid Effect (SE), Cross Effect (CE) and Thermal Mixing (TM) DNP. In a recent study we revisited the solid state SE-DNP mechanism at high magnetic fields, using a spin density operator description involving spin relaxation, for the case of an isolated electron spin interacting with neighboring nuclei. In this publication we extend this study by considering the hyper-polarization of nuclei in systems containing two interacting electrons. In these spin systems both processes SE-DNP and CE-DNP are simultaneously active. As previously, a quantum description taking into account spin relaxation is used to calculate the dynamics of spin systems consisting of interacting electron pairs coupled to (core) nuclei. Numerical simulations are used to demonstrate the dependence of the SE- and CE-DNP enhancements on the MW irradiation power and frequency, on electron, nuclear and cross relaxation mechanisms and on the spin interactions. The influence of the presence of many nuclei on the hyper-polarization of an individual core nucleus is examined, showing the similarities between the two DNP processes. These studies also indicate the advantages of the CE- over the SE-DNP processes, both driving the polarization of the bulk nuclei, via the nuclear dipole-dipole interactions. © 2011 Elsevier Inc. All rights reserved.
Pulsed dipolar spectroscopy distance measurements in biomacromolecules labeled with Gd(III) markersY. Song | T. J. Meade | A. V. Astashkin | E. L. Klein | J. H. Enemark | A. Raitsimring
Volume 210, Issue 1, May 2011, Pages 59-68
This work demonstrates the feasibility of using Gd(III) tags for long-range Double Electron Electron Resonance (DEER) distance measurements in biomacromolecules. Double-stranded 14- base pair Gd(III)-DNA conjugates were synthesized and investigated at Ka band. For the longest Gd(III) tag the average distance and average deviation between Gd(III) ions determined from the DEER time domains was about 59 ± 12 . This result demonstrates that DEER measurements with Gd(III) tags can be routinely carried out for distances of at least 60 , and analysis indicates that distance measurements up to 100 are possible. Compared with commonly used nitroxide labels, Gd(III)-based labels will be most beneficial for the detection of distance variations in large biomacromolecules, with an emphasis on large scale changes in shape or distance. Tracking the folding/unfolding and domain interactions of proteins and the conformational changes in DNA are examples of such applications. © 2011 Elsevier Inc. All rights reserved.
Second order gradient ascent pulse engineeringP. De Fouquieres | S. G. Schirmer | S. J. Glaser | Ilya Kuprov
Volume 212, Issue 2, October 2011, Pages 412-417
We report some improvements to the gradient ascent pulse engineering (GRAPE) algorithm for optimal control of spin ensembles and other quantum systems. These include more accurate gradients, convergence acceleration using the Broyden-Fletcher-Goldfarb-Shanno (BFGS) quasi-Newton algorithm as well as faster control derivative calculation algorithms. In all test systems, the wall clock time and the convergence rates show a considerable improvement over the approximate gradient ascent. © 2011 Elsevier Inc. All rights reserved.
Magnetic particle imaging (MPI) for NMR and MRI researchersEmine U. Saritas | Patrick W. Goodwill | Laura R. Croft | Justin J. Konkle | Kuan Lu | Bo Zheng | Steven M. Conolly
Volume 229, Issue , April 2013, Pages 116-126
Magnetic Particle Imaging (MPI) is a new tracer imaging modality that is gaining significant interest from NMR and MRI researchers. While the physics of MPI differ substantially from MRI, it employs hardware and imaging concepts that are familiar to MRI researchers, such as magnetic excitation and detection, pulse sequences, and relaxation effects. Furthermore, MPI employs the same superparamagnetic iron oxide (SPIO) contrast agents that are sometimes used for MR angiography and are often used for MRI cell tracking studies. These SPIOs are much safer for humans than iodine or gadolinium, especially for Chronic Kidney Disease (CKD) patients. The weak kidneys of CKD patients cannot safely excrete iodine or gadolinium, leading to increased morbidity and mortality after iodinated X-ray or CT angiograms, or after gadolinium-MRA studies. Iron oxides, on the other hand, are processed in the liver, and have been shown to be safe even for CKD patients. Unlike the "black blood" contrast generated by SPIOs in MRI due to increased T2 dephasing, SPIOs in MPI generate positive, "bright blood" contrast. With this ideal contrast, even prototype MPI scanners can already achieve fast, high-sensitivity, and high-contrast angiograms with millimeter-scale resolutions in phantoms and in animals. Moreover, MPI shows great potential for an exciting array of applications, including stem cell tracking in vivo, first-pass contrast studies to diagnose or stage cancer, and inflammation imaging in vivo. So far, only a handful of prototype small-animal MPI scanners have been constructed worldwide. Hence, MPI is open to great advances, especially in hardware, pulse sequence, and nanoparticle improvements, with the potential to revolutionize the biomedical imaging field. © 2012 Elsevier Inc. All rights reserved.
Ultra-high resolution in MAS solid-state NMR of perdeuterated proteins: Implications for structure and dynamicsBernd Reif
Volume 216, Issue , March 2012, Pages 1-12
High resolution proton spectra are obtained in MAS solid-state NMR in case samples are prepared using perdeuterated protein and D 2O in the recrystallization buffer. Deuteration reduces drastically 1H, 1H dipolar interactions and allows to obtain amide proton line widths on the order of 20 Hz. Similarly, high-resolution proton spectra of aliphatic groups can be obtained if specifically labeled precursors for biosynthesis of methyl containing side chains are used, or if limited amounts of H 2O in the bacterial growth medium is employed. This review summarizes recent spectroscopic developments to access structure and dynamics of biomacromolecules in the solid-state, and shows a number of applications to amyloid fibrils and membrane proteins. © 2012 Elsevier Inc. All rights reserved.
Fast passage dynamic nuclear polarization on rotating solidsFrederic Mentink-Vigier | Ümit Akbey | Yonatan Hovav | Shimon Vega | Hartmut Oschkinat | Akiva Feintuch
Volume 224, Issue , November 2012, Pages 13-21
Magic Angle Spinning (MAS) Dynamic Nuclear Polarization (DNP) has proven to be a very powerful way to improve the signal to noise ratio of NMR experiments on solids. The experiments have in general been interpreted considering the Solid-Effect (SE) and Cross-Effect (CE) DNP mechanisms while ignoring the influence of sample spinning. In this paper, we show experimental data of MAS-DNP enhancements of 1H and 13C in proline and SH3 protein in glass forming water/glycerol solvent containing TOTAPOL. We also introduce a theoretical model that aims at explaining how the nuclear polarization is built in MAS-DNP experiments. By using Liouville space based simulations to include relaxation on two simple spin models, {electron-nucleus} and {electron-electron-nucleus}, we explain how the basic MAS-SE-DNP and MAS-CE-DNP processes work. The importance of fast energy passages and short level anti-crossing is emphasized and the differences between static DNP and MAS-DNP is explained. During a single rotor cycle the enhancement in the {electron-electron-nucleus} system arises from MAS-CE-DNP involving at least three kinds of two-level fast passages: an electron-electron dipolar anti-crossing, a single quantum electron MW encounter and an anti-crossing at the CE condition inducing nuclear polarization in- or decrements. Numerical, powder-averaged, simulations were performed in order to check the influence of the experimental parameters on the enhancement efficiencies. In particular we show that the spinning frequency dependence of the theoretical MAS-CE-DNP enhancement compares favorably with the experimental 1H and 13C MAS-DNP enhancements of proline and SH3. © 2012 Elsevier Inc. All rights reserved.
Similarity of SABRE field dependence in chemically different substratesEibe B. Dücker | Lars T. Kuhn | Kerstin Münnemann | Christian Griesinger
Volume 214, Issue , January 2012, Pages 159-165
The Non-Hydrogenative Parahydrogen-Induced Polarization (NH-PHIP) technique, which is referred to as Signal Amplification by Reversible Exchange (SABRE), has been reported to be applicable to various substrates and catalysts. For more detailed studies, pyridine was mainly examined in the past. Here, we examined several pyrazole derivatives towards their amenability to this method using Crabtree's Catalyst, which is the polarization transfer catalyst that is best documented. Additionally, the dependence of the signal enhancement on the field strength, at which the polarization step takes place, was examined for pyridine and four different pyrazoles. To achieve this, the polarization step was performed at numerous previously determined magnetic fields in the stray field of the NMR spectrometer. The substrate dependence of the field dependence proved to be relatively small for the different pyrazoles and a strong correlation to the field dependence for pyridine was observed. Reducing the number of spins in the catalyst by deuteration leads to increased enhancement. This indicates that more work has to be invested in order to be able to reproduce the SABRE field dependence by simulations. © 2011 Elsevier Inc. All rights reserved.
BaBa-xy16: Robust and broadband homonuclear DQ recoupling for applications in rigid and soft solids up to the highest MAS frequenciesKay Saalwächter | Frank Lange | Krzysztof Matyjaszewski | Chih Feng Huang | Robert Graf
Volume 212, Issue 1, September 2011, Pages 204-215
We here present a substantially improved version of the popular Back-to-Back (BaBa) homonuclear double-quantum (DQ) MAS recoupling pulse sequence. By combining the original pulse sequence with a virtual π pulse train with xy-16 phase cycling along with time-reversed DQ reconversion, a truly broadband and exceptionally robust pulse sequence is obtained. The sequence has moderate radio-frequency power requirements, amounting to only one 360° nutation per rotor cycle, it is robust with respect to rf power and tune-up errors, and its broadband performance increases with increasing spinning frequency, here tested up to 63 kHz. The experiment can be applied to many spin-1/2 nuclei in rigid solids with substantial frequency offsets and CSAs, which is demonstrated on the example of 31P NMR of a magnesium ultraphosphate, comparing experimental data with multi-spin simulations, and we also show simulations addressing the performance in 13C NMR of bio(macro)molecules. 1H-based studies of polymer dynamics are highlighted for the example of a rigid solid with strongly anisotropic mobility, represented by a polymer inclusion compound, and for the example of soft materials with weak residual dipole-dipole couplings, represented by homogeneous and inhomogeneous elastomers. We advocate the use of normalized (relaxation-corrected) DQ build-up curves for a quantitative assessment of weak average dipole-dipole couplings and even distributions thereof. © 2011 Elsevier Inc. All rights reserved.
Toward single-shot pure-shift solution 1H NMR by trains of BIRD-based homonuclear decouplingAdonis Lupulescu | Gregory L. Olsen | Lucio Frydman
Volume 218, Issue , May 2012, Pages 141-146
Achieving homonuclear 1H decoupling remains one of the key challenges in liquid-state NMR. Such spectra would endow a variety of organic and analytical applications with an increased resolution, and would ideally do so even in a one-dimensional format. A number of parallel efforts aimed at achieving this goal using two-dimensional acquisitions have been proposed; approaches demonstrated over recent years include, among others, new modes for achieving purely-absorptive J spectroscopy, the use of spatially-selective manipulations, and exploiting the natural spin dilution afforded by heteronuclei. The present study relies on the latter approach, and explores the use of BIRD pulses distinguishing between protons bonded to 13C from those bonded to 12C, to achieve homonuclear decoupling in a continuous 1D scan. Studies on several representative compounds demonstrate that this goal can be implemented in a robust format, provided that suitable care is also taken to suppress unwanted coherences, of making all manipulations sufficiently broad-banded, and to provide adequate heteronuclear decoupling of the targeted protons. Dependable homonuclear decoupling performance can then be achieved, with minimal line width, fine-tuning, and sensitivity penalties. © 2012 Elsevier Inc. All rights reserved.
A portable Halbach magnet that can be opened and closed without force: The NMR-CUFFCarel W. Windt | Helmut Soltner | Dagmar Van Dusschoten | Peter Blümler
Volume 208, Issue 1, January 2011, Pages 27-33
Portable equipment for nuclear magnetic resonance (NMR) is becoming increasingly attractive for use in a variety of applications. One of the main scientific challenges in making NMR portable is the design of light-weight magnets that possess a strong and homogeneous field. Existing NMR magnets can provide such magnetic fields, but only for small samples or in small regions, or are rather heavy. Here we show a simple yet elegant concept for a Halbach-type permanent magnet ring, which can be opened and closed with minimal mechanical force. An analytical solution for an ideal Halbach magnet shows that the magnetic forces cancel if the structure is opened at an angle of 35.3° relative to its poles. A first prototype weighed only 3.1 kg, and provided a flux density of 0.57 T with a homogeneity better than 200 ppm over a spherical volume of 5 mm in diameter without shimming. The force needed to close it was found to be about 20 N. As a demonstration, intact plants were imaged and water (xylem) flow measured. Magnets of this type (NMR-CUFF = Cut-open, Uniform, Force Free) are ideal for portable use and are eminently suited to investigate small or slender objects that are part of a larger or immobile whole, such as branches on a tree, growing fruit on a plant, or non-metallic tubing in industrial installations. This new concept in permanent-magnet design enables the construction of openable, yet strong and homogeneous magnets, which aside from use in NMR or MRI could also be of interest for applications in accelerators, motors, or magnetic bearings. © 2010 Elsevier Inc. All rights reserved.
Homonuclear decoupling for enhancing resolution and sensitivity in NOE and RDC measurements of peptides and proteinsJinfa Ying | Julien Roche | Ad Bax
Volume 241, Issue 1, January 2014, Pages 97-102
Application of band-selective homonuclear (BASH) 1H decoupling pulses during acquisition of the 1H free induction decay is shown to be an efficient procedure for removal of scalar and residual dipolar couplings between amide and aliphatic protons. BASH decoupling can be applied in both dimensions of a homonuclear 2D NMR experiment and is particularly useful for enhancing spectral resolution in the HN-Hα region of NOESY spectra of peptides and proteins, which contain important information on the backbone torsion angles. The method then also prevents generation of zero quantum and HzN-Hzα terms, thereby facilitating analysis of intraresidue interactions. Application to the NOESY spectrum of a hexapeptide fragment of the intrinsically disordered protein α-synuclein highlights the considerable diffusion anisotropy present in linear peptides. Removal of residual dipolar couplings between HN and aliphatic protons in weakly aligned proteins increases resolution in the 1H-15N HSQC region of the spectrum and allows measurement of RDCs in samples that are relatively strongly aligned. The approach is demonstrated for measurement of RDCs in protonated 15N/13C-enriched ubiquitin, aligned in Pf1, yielding improved fitting to the ubiquitin structure. © Published by Elsevier Inc.
Optimization of SABRE for polarization of the tuberculosis drugs pyrazinamide and isoniazidHaifeng Zeng | Jiadi Xu | Joseph Gillen | Michael T. McMahon | Dmitri Artemov | Jean Max Tyburn | Joost A B Lohman | Ryan E. Mewis | Kevin D. Atkinson | Gary G R Green | Simon B. Duckett | Peter C M Van Zijl
Volume 237, Issue , October 2013, Pages 73-78
Hyperpolarization produces nuclear spin polarization that is several orders of magnitude larger than that achieved at thermal equilibrium thus providing extraordinary contrast and sensitivity. As a parahydrogen induced polarization (PHIP) technique that does not require chemical modification of the substrate to polarize, Signal Amplification by Reversible Exchange (SABRE) has attracted a lot of attention. Using a prototype parahydrogen polarizer, we polarize two drugs used in the treatment of tuberculosis, namely pyrazinamide and isoniazid. We examine this approach in four solvents, methanol-d4, methanol, ethanol and DMSO and optimize the polarization transfer magnetic field strength, the temperature as well as intensity and duration of hydrogen bubbling to achieve the best overall signal enhancement and hence hyperpolarization level. © 2013 Elsevier Inc. All rights reserved.
Monte Carlo simulation and theory of proton NMR transverse relaxation induced by aggregation of magnetic particles used as MRI contrast agentsQuoc Lam Vuong | Pierre Gillis | Yves Gossuin
Volume 212, Issue 1, September 2011, Pages 139-148
Superparamagnetic particles are widely used in MRI as R 2 contrast agents. In this last decade, different studies have focused on aggregation of superparamagnetic particles for important applications such as multimodal agents. A complete study - via simulations - of the influence of aggregation on the MR efficiency of these particles at high magnetic field is presented here. First, an empirical expression is proposed for R 2 in the presence of uniformly distributed nanoparticles, taking into account two regimes at once (motional averaging and slow motion regimes). Three cluster shapes are simulated: Sphere, shell and line. An analytical model is proposed to understand water transverse relaxation induced by spherical and shell aggregates. Simulations lead to the conclusion that, in the motional averaging regime, the most efficient aggregate contrast agent is the densest sphere or shell. © 2011 Elsevier Inc. All rights reserved.
Level anti-crossings in ParaHydrogen Induced Polarization experiments with Cs-symmetric moleculesL. Buljubasich | M. B. Franzoni | H. W. Spiess | K. Münnemann
Volume 219, Issue , June 2012, Pages 33-40
Hyperpolarization by means of ParaHydrogen Induced Polarization (PHIP) has found increasing applications since its discovery. However, in the last decade only a few experiments have been reported describing the hydrogenation of symmetric molecules. A general AA′BB′ system is studied here. Calculations of the spin dynamics with the density matrix formalism support the experimental findings, providing profound understanding of the experiments in Cs-symmetric molecules. Level anti-crossings between states related to the triplet and the singlet state of one pair of the protons are identified as being responsible for hyperpolarization transfer in a PHIP experiment, when the former p-H2 protons occupy the sites AA′. The hydrogenation of acetylene dicarboxylic acid dimethylester with parahydrogen is used to illustrate the case. The theoretical treatment applied to this particular reaction explains the signal enhancements in both groups of protons in the spectrum when the sample is placed in the proper magnetic field strength, including the phase inversion of the signal of the methyl group. The treatment described here can be extended to every molecule which can be approximated as an AA′BB′ system. © 2012 Elsevier Inc. All rights reserved.
New methods for the acquisition of ultra-wideline solid-state NMR spectra of spin-1/2 nuclidesAlan W. MacGregor | Luke A. O'Dell | Robert W. Schurko
Volume 208, Issue 1, January 2011, Pages 103-113
The Wideband Uniform Rate Smooth Truncation - Carr-Purcell Meiboom-Gill (WURST-CPMG) pulse sequence was recently introduced as a new method of acquiring ultra-wideline solid-state NMR (SSNMR) patterns of quadrupolar nuclei (Chem. Phys. Lett. 464 (2008) 97). Herein, we describe the application of the WURST-CPMG pulse sequence to stationary samples (i.e., non-spinning or "static" samples) of various spin-1/2 nuclides (119Sn, 207Pb, 199Hg and 195Pt) in order to examine its effectiveness for acquiring ultra-wideline SSNMR patterns. WURST-CPMG is compared to the CPMG and Cross Polarization (CP)-CPMG pulse sequences in select cases (119Sn and 207Pb, respectively), and its usefulness in obtaining ultra-wideline SSNMR spectra in a piecewise fashion is explored. In addition, a preliminary investigation of pulses generated using optimal control theory (OCT) for the purpose of wideline excitation is presented; spectra acquired using these pulses are compared with standard, rectangular pulses of similar pulse powers. Both methods show much promise for acquiring high quality wideline patterns dominated by chemical shift anisotropy, with minimal distortions and significantly reduced experimental times. © 2010 Elsevier Inc. All rights reserved.
Effect of freezing conditions on distances and their distributions derived from Double Electron Electron Resonance (DEER): A study of doubly-spin-labeled T4 lysozymeElka R. Georgieva | Aritro S. Roy | Vladimir M. Grigoryants | Petr P. Borbat | Keith A. Earle | Charles P. Scholes | Jack H. Freed
Volume 216, Issue , March 2012, Pages 69-77
Pulsed dipolar ESR spectroscopy, DEER and DQC, require frozen samples. An important issue in the biological application of this technique is how the freezing rate and concentration of cryoprotectant could possibly affect the conformation of biomacromolecule and/or spin-label. We studied in detail the effect of these experimental variables on the distance distributions obtained by DEER from a series of doubly spin-labeled T4 lysozyme mutants. We found that the rate of sample freezing affects mainly the ensemble of spin-label rotamers, but the distance maxima remain essentially unchanged. This suggests that proteins frozen in a regular manner in liquid nitrogen faithfully maintain the distance-dependent structural properties in solution. We compared the results from rapidly freeze-quenched (≤100 μs) samples to those from commonly shock-frozen (slow freeze, 1 s or longer) samples. For all the mutants studied we obtained inter-spin distance distributions, which were broader for rapidly frozen samples than for slowly frozen ones. We infer that rapid freezing trapped a larger ensemble of spin label rotamers; whereas, on the time-scale of slower freezing the protein and spin-label achieve a population showing fewer low-energy conformers. We used glycerol as a cryoprotectant in concentrations of 10% and 30% by weight. With 10% glycerol and slow freezing, we observed an increased slope of background signals, which in DEER is related to increased local spin concentration, in this case due to insufficient solvent vitrification, and therefore protein aggregation. This effect was considerably suppressed in slowly frozen samples containing 30% glycerol and rapidly frozen samples containing 10% glycerol. The assignment of bimodal distributions to tether rotamers as opposed to protein conformations is aided by comparing results using MTSL and 4-Bromo MTSL spin-labels. The latter usually produce narrower distance distributions. © 2012 Elsevier Inc. All rights reserved.
Solution NMR spectroscopy of supra-molecular systems, why bother? A methyl-TROSY viewLewis E. Kay
Volume 210, Issue 2, June 2011, Pages 159-170
With the development of appropriate labeling schemes and the associated experiments that exploit them it has become possible to record high quality solution NMR spectra of supra-molecular complexes with molecular masses extending to 1 MDa. One such approach involves selective 13CH 3 methyl labeling in highly deuterated proteins using experiments that make use of a methyl-TROSY effect that significantly improves both resolution and sensitivity in spectra. The utility of this methodology has been demonstrated on a growing number of interesting particles. It seems appropriate at this juncture, therefore, to 'step back' and evaluate the role that solution NMR spectroscopy can play in what has traditionally been the domain of X-ray crystallography and more recently cryo-electron microscopy. It is argued here that solution NMR can make a critical contribution to our understanding of how dynamics regulate function in these high molecular weight systems. Several examples from work in my laboratory on the proteasome are presented as an illustration. © 2011 Elsevier B.V. All rights reserved.
Speeding up the measurement of one-bond scalar ( 1J) and residual dipolar couplings ( 1D) by using non-uniform sampling (NUS)Christina M. Thiele | Wolfgang Bermel
Volume 216, Issue , March 2012, Pages 134-143
The accurate and precise measurement of one-bond scalar and residual dipolar coupling (RDC) constants is of prime importance to be able to use RDCs for structure determination. If coupling constants are to be extracted from the indirect dimension of HSQC spectra a significant saving of measurement time can be achieved by non-uniform sampling (NUS). Coupling constants can either be obtained with the same precision as in traditionally acquired spectra in a fraction of the measurement time or the precision can be significantly improved if the same amount of measurement time as for traditionally acquired spectra is invested. The application of NUS for the measurement of 1J (scalar coupling constants) and 1T (total couplings constants) from different kinds of ω 1-coupled spectra (including also J-scaled ones) is examined in detail and the possible gains in time or resolution are discussed. When using the newly proposed compressed sensing (CS) algorithm for processing, the quality of the spectra is comparable to the traditionally sampled ones. © 2012 Elsevier Inc. All rights reserved.
Chemical exchange in biomacromolecules: Past, present, and futureArthur G. Palmer
Volume 241, Issue 1, January 2014, Pages 3-17
The perspective reviews quantitative investigations of chemical exchange phenomena in proteins and other biological macromolecules using NMR spectroscopy, particularly relaxation dispersion methods. The emphasis is on techniques and applications that quantify the populations, interconversion kinetics, and structural features of sparsely populated conformational states in equilibrium with a highly populated ground state. Applications to folding, molecular recognition, catalysis, and allostery by proteins and nucleic acids are highlighted. © 2014 Elsevier Inc. All rights reserved.
Quantitative solid-state 13C NMR with signal enhancement by multiple cross polarizationRobert L. Johnson | Klaus Schmidt-Rohr
Volume 239, Issue , February 2014, Pages 44-49
A simple new method is presented that yields quantitative solid-state magic-angle spinning (MAS) 13C NMR spectra of organic materials with good signal-to-noise ratios. It achieves long (>10 ms) cross polarization (CP) from 1H without significant magnetization losses due to relaxation and with a moderate duty cycle of the radio-frequency irradiation, by multiple 1-ms CP periods alternating with 1H spin-lattice relaxation periods that repolarize the protons. The new method incorporates previous techniques that yield less distorted CP/MAS spectra, such as a linear variation ("ramp") of the radio-frequency field strength, and it overcomes their main limitation, which is T1ρ relaxation of the spin-locked 1H magnetization. The ramp of the radio-frequency field strength and the asymptotic limit of cross polarization makes the spectral intensity quite insensitive to the exact field strengths used. The new multiCP pulse sequence is a "drop-in" replacement for previous CP methods and produces no additional data-processing burden. Compared to the only reliable quantitative 13C NMR method for unlabeled solids previously available, namely direct-polarization NMR, the measuring time is reduced by more than a factor of 50, enabling higher-throughput quantitative NMR studies. The new multiCP technique is validated with 14-kHz MAS on amino-acid derivatives, plant matter, a highly aromatic humic acid, and carbon materials made by low-temperature pyrolysis. © 2013 Elsevier Ltd. All rights reserved.


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A VALUABLE RESOURCE GUIDE FOR HEALING AND ASCENSION ACTIVATION Within us lies the full potential for the total healing and resolution of all duality programs as they surface or appear in our daily life, and I say it is an appearance because it has become a crystallized illusion and part of the old, limited, conditioned human matrix in this third dimension. With the current two-strand DNA, we have no memory of where we came from, we are disconnected from source, and therefore we are entrapped in the illusions of the outer world machinery and its Matrix, and all the stimulus we are programmed and bombarded with daily. With only the two-strand DNA, we become enslaved, conditioned, and dependent in dysfunctional, socio-economic institutions, religions, and false belief systems that never truly deliver the freedom or liberation they claim to. The true END-GAME of each soul's journey is to free themselves from this conditioned state of duality once and for all, and return to the Source from which we originally manifested, while still in a physical 3D body. You can call this original source God, Higher Self, Unconditional Divine Love or unlimited freedom. You can achieve this freedom by utilizing your original, God-given Divine Intelligence, through the power of Divine Love, and regain your lost Will by reactivating your original, unlimited sleeping DNA codes. It is man's eternal quest to seek ways of becoming one with the universal Source of creation, to understand its higher power and will, its intelligence, and its driving force in all creation in relationship to each embodied soul. The only difference between this supreme Power-Divine Intelligence and Man is the separation or split that exists within the modified matrix of our old DNA Codes, out of which the illusion of duality was first orchestrated. The collective fracture of humanity's denied unconscious shadow self is the unhealed inner child of us all. We live in a world of conflicting and seemingly opposing forces, or what appear as opposing forces right within our own being as well as externalized in our daily reality. Opposing forces such as light-dark, good-bad, pleasure-pain, love-fear, well-being-dis-ease, birth-death, joy-sorrow, entrapment-liberation, etc. shape our everyday lives and the choices we make. How we make peace and resolve these opposing forces within our own being determines the quality of life we experience. "God/Goddess, the Divine Universal Intelligence within each of us, utilizes the projection and illusion of duality in 3rd and 4th density (dimension) as a catalyst for souls to evolve and awaken. Each human being has these duality codes imbedded in their DNA. Now is the time of awakening our potential to activate our original unlimited DNA blueprint as we master the illusion of duality through the process of discernment and awakening to our enlightenment, healing, and ascension. We are no longer victims of an "outer force or reality" controlling us, but quickly becoming Victors of our own destiny as we merge the gap between inner and outer, light and dark, love and fear, right within the matrix of our own consciousness and psyche. In this book, you will find many valuable healing tools that will help you to reactivate your original DNA blueprint and manifest your higher potential by reconnecting with your greater Self and your Divine Purpose.
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Scott, at 18/01 I shared your art in my blog too... Since i'm not an US american (south american, more precisely brazilian), I chose one of the many protests around US to let people in Brazil know that we can have a new heavy censorship in the most influent media of our times.
And now, if PIPA will really be aprooved, because of my blog, blogspot.com can be blocked in USA
Any of you heard about ACTA? It's just as bad, and it's international click here to sign a petition against it if you wishhttp://www.avaaz.org/en/eu_save_the_internet_spread/?tta


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On Tuesday, Nielsen Holdings (NYSE: NLSN ) announced it will acquire all outstanding shares of international media and marketing research firm Arbitron (UNKNOWN: ARB.DL ) in an all-cash transaction, paying $48 per share. The boards of both companies have already approved the merger.
In a statement on the $1.2 billion merger, Nielsen boasted of the "unique radio measurement and listening information" that Arbitron will bring to Nielsen, and how bringing the two companies together will "enable broader measurement of consumer media behavior in more markets around the world."
Together, Nielsen says the company will generate $6 billion in combined annual revenue, with earnings before interest, taxes, depreciation, and amortization amounting to approximately $1.7 billion. Nielsen further says it expects to find $20 million worth of "cost synergies" that can be cut once the merger is complete.
Shares of Arbitrion were up nearly 24% in response to the announcement at $47.01 per share. Nielsen's shares, too, moved up 4% at $30.80.


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Asian Defence News at Asian Defense and chinese defence and indian defence news and indian defense news and Russian defence news and chinese defence news at asian defence news
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Has laoreet percipitur ad. Vide interesset in mei, no his legimus verterem. Et nostrum imperdiet appellantur usu, mnesarchum referrentur id vim.
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Shadowfax is becoming a neat freak very slowly, had been a bit of a piggy. A quiet gentleman, patient and not expecting much. Just wants to get his fair share and won't bother anyone for more. Has many sterling qualities. Not the least bit cage aggressive, drinks from a bowl, adores his hay, meticulously always uses same corner for 'output.'
Tiffany is a power chewer. Very, very sweet, loves people. Positive attitude. Well socialized with other rabbits. Hay monster deluxe, loves chewing the heavy duty stuff like Oxbow Bungalows and Busy Bunny willow balls. Drinks from a bowl, not a big drinker.
Please review our shelter info and policies right here on Adopt A Pet and also on our website. Please no inquiries from minors or folks in transient situations.
Thank You!
Your message has been sent to Bright Eyes Sanctuary.
You'll receive a copy, too, at to help you keep track of which pets you've inquired about, and which shelters and rescues you've emailed.
NOTE: Some shelters have physical locations you can visit; some of these shelters may only have pets for a limited time, so please do not wait for a reply—just go visit the shelter! Other organizations are rescue groups run by busy volunteers who may take a while to reply. You can find information about the shelter or rescue group caring for this pet, and their adoption procedures, on the pet's details page on Adopt-a-Pet.com.


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Potato price hits highest for 20 years
Published 05/12/2012 | 06:00
A sharp fall in the number of acres sown to potatoes, combined with a yield reduction of 20-30pc have pushed the price of the humble spud to its highest level in over 20 years.
Potatoes are currently making €500-600/t bulk ex-farm, almost triple what they were worth at the same time last year.
However, the current high prices are only just enough to help potato growers out of the debts they incurred in the past two years, the IFA maintained.
"Potato growers lost around €25m last year, selling their crop for just €140-180/t bulk," said IFA potato expert Matt Molloy.
"What they are getting for spuds this year will just about get them out of the hole they were in for the past few years."
With around 85pc of the national potato harvest now complete, it looks like potato yields for this year are down 20-30pc.
Cold and wet weather cut both the number and size of potato tubers on each plant, slashing the tonnage of spuds harvested per acre.
The yield reduction came on top of a 25pc fall in the acreage sown to potatoes as growers took 7,500ac of the crop out of production.
"Growers simply got out because they couldn't make any profit as the supermarket multiples engaged in prices wars on potatoes and other vegetables," maintained Mr Molloy.
The shortfall in potato supplies in Ireland is being mirrored elsewhere in Europe after the weather took a major toll on crops.
The North-Western European Potato Growers Association has predicted a 16.7pc fall in the amount of potatoes harvested in Germany, Belgium, France, Britain and the Netherlands in 2012 compared to 2011.
The fall is the equivalent of a 10cp reduction on the five-year average for those countries.
The potato harvest in Britain is expected to finish 25pc lower than 2011, because of a five per cent reduction in acreage and poor yields of 37.4t/ha.
Indo Farming


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With a major national election just months away, the stakes continue to get higher. A well-educated workforce is the key to pulling the country out of the present economic doldrums. But in election contests nationwide, and particularly in my home state of Louisiana, improving public education is rarely if ever mentioned as a campaign issue.
by Jim Brown
Kids all over America graduated last week from kindergarten, grade school, high school, college and graduate schools. And there was always a commencement speaker. Most of you will never give a commencement address. But as a public official, I was called on to give a number of them. And guess what? I don't remember any of the advice that I gave to these newly minted graduates.
It's that time of year for college bowl games and NFL playoffs. And when you look at the winners, it's all about the coach. Top college teams like Alabama and LSU don't build winning programs by setting up boards and committees. The job of producing a winner is on the shoulders of the coach and no one else. If he wins, he gets a big bonus. If he loses, he gets fired.
by Tom Aswell, publisher of Louisiana Voice
"They're still negotiating with the terrorists."
That gem, said in a private email to LouisianaVoice, came from a blogger who is relative new on the scene but who is very perceptive about what the Bobby administration is trying to do to higher education.https://lahigheredconfessions.wordpress.com/2015/02/27/open-letter-to-higher-education-leaders-the-time-for-negotiating-is-over/
Unfortunately, crisis budgeting is not synonymous with making the delivery of Louisiana higher education efficient or even effective, although it can serve as a starting point for that overdue process. Problem is, that process should have started long ago.
by John Kennedy, Louisiana Treasurer
At least three times a year, I clear my calendar and volunteer to substitute teach. I try to head back to the classroom on a regular basis-not because I need a crash course on the Pythagorean Theorem or the latest sixth grade gossip-but because I want to know what it's like to stand in front of students and help them learn.
While the Governor's focus, as of late, has been on a different legal matter, Common Core, Bobby Jindal's primary education achievement, his education reform will meet its sternest test--the Louisiana Supreme Court.
by Lamar White Jr., Publisher of CenLamar
First published on CenLamar.com
In the late 1950s, Louisiana Governor Earl K. Long once said that the state's attorney general didn't know the difference between a jumpsuit and a lawsuit. "If you want to hide something from Jack Gremillion," he said, "put it in a law book."
In many ways, today, might be called the first day of the legislative seession 2014 as Governor Bobby Jindal submitted a balanced budget proposal to the Legislature. Here is the Jindal press release that explains the Executive budget and the governor's priorities.
As Louisiana progresses through its second year of its statewide Student Scholarships for Education Excellence program, data produced still can't reveal whether the program is improving significantly the lot of children or what effect if any it may have, even as it succeeds on a cost basis.


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Global climate change is a working-class issue
The 60th Annual United Nations Nongovernmental Organization (NGO) Conference took place at the UN headquarters in New York in early September. The conference focused on climate change as one of the key issues facing the world
Zones of conflict: challenge to African unity
There is a new danger plaguing Africa: zones of conflict are expanding into regional wars that take an ever-increasing human toll.
Rethinking Toyota, autoworkers and allies
People who own Toyotas are either the moral equivalent of scabs or they are one of the most potentially powerful allies U.S. organized labor has today.


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Black entrepreneurs told to advocate, lobby
Indianapolis shows how, speaker says
Indianapolis last year was ranked among the top 10 cities for African-American entrepreneurs. Milwaukee was nowhere on the list, although the two cities 18 years ago mirrored each other in unemployment and minority business development.
If Indy can do it, so can Brew City, said Harry C. Alford, president and chief executive of the National Black Chamber of Commerce.
"Indianapolis is one of the most progressive, economically equitable cities in the United States, and I'm going to take credit for it," said Alford, former president of the Indianapolis Black Chamber of Commerce and one of the nation's most influential small-business advocates. "That's because we became economically diverse. That's because we employed people."
Alford, a champion of black business empowerment, spoke Thursday at the Wisconsin African-American Women's Center, 3020 W. Vliet St. About 50 people attended the event, sponsored by the Wisconsin Black Chamber of Commerce.
During his speech, Alford cited a 1990 census study that ranked Milwaukee and Indianapolis among the most segregated cities in the United States. While Indianapolis has outpaced Milwaukee in employment and business opportunities for African-Americans, with leadership such benchmarks can be duplicated here, he said.
"I heard about this unemployment picture for Milwaukee. It doesn't have to be this way," Alford said. "If Indianapolis can do it, Milwaukee can do it."
Alford said Indianapolis has benefited from a thriving minority business community, a vibrant black middle class and an overall business climate that encourages entrepreneurship, all of which have contributed to the growth of black-owned businesses.
In 1991, Alford founded Indianapolis' first black chamber of commerce. He immediately drew support from black business leaders who donated the money and resources he needed to operate the chamber.
"I needed to find a group that could apply pressure," he said. "The city had a Hispanic chamber of commerce, yet with a black population of 250,000, there wasn't a single black business association."
Under Alford's leadership, the Indianapolis chamber took the lead on diversifying procurement opportunities with city and state governments, major corporations, hospitals and airports.
The chamber's efforts paved the way for a more open and inclusive procurement process for minority contractors at all levels of government.
"We just made it an issue and demanded progress," he said.
Alford spent two years as president of Indianapolis' black chamber before founding the National Black Chamber of Commerce in 1993. He ran the organization from Indianapolis until moving it to Washington, D.C., in 1994.
"Here in Milwaukee, you're still in a rut," he said. "If Milwaukee wants to be a thriving city, a D.C., an Indy or an Atlanta as opposed to a Detroit, it has to come through the efforts of African-Americans."
Business Wire
Recent national business updates from the Associated Press


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AT&T Says NYC is 'Not Ready' For the iPhone [UPD]
Those if you in New York City trying to get an iPhone by ordering from AT&T online will know that it's just not possible right now. For one reason or another, those trying to buy online using a New York City zip code will find that the iPhone is not available in their area.
The Consumerist did some digging behind such claims and verified that AT&T has frozen online sales of the iPhone in NYC. (It does seem, however, that retail sales are continuing as normal.) The following is an online help desk conversation between the Consumerist's Laura Northrup and AT&T sales support:
Daphne: Welcome to AT&T online Sales support. How may I assist you with placing your order today?
Laura: Hi, I was looking at the iPhone 3Gs and the system tells me that I cannot order one in my ZIP code. My zip code is 11231. (Brooklyn, NY) Is this true? Are iPhones no longer available in New York City?
Daphne: I am happy to be helping you today . Yes, this is correct the phone is not offered to you because New York is not ready for the iPhone.
Daphne: You don't have enough towers to handle the phone.
Laura: Thank you for your help. So the phone is not available to people anywhere in the city?
Daphne: Yes this is correct Laura.
This sort of news is startling in light of service complaints in U.S. cities such as NYC and San Francisco – where there are high concentrations of iPhone users.
AT&T responded by issuing the statement, "We periodically modify our promotions and distribution channels," which basically tells us nothing other than confirming that sales have indeed been frozen in NYC.
Update: it appears that New York City is once again ready for the iPhone, as online sales via AT&T's website are now possible again for those living in the city that never sleeps.
subliminalaffectIt's about time that att gets their just desserts by having their shady business practices and bloated, overpriced and underperforming services exposed. This company has been trash over the past decade and more and more of the public is waking up to this via verizon's help and their own self deterioration thanks to greed. God bless the Iphone!!!
GlorianThe iphone would kill any network in high concentration cities filled with yuppies who feel they need to have the latest trend electronics. Even if AT&T was ready it is still amazing the amount of people who "need" this novelty.
I also think they are taking "NY isn't ready" a bit too serious, because it is in fact AT&T that's not ready. It's like someone asking me if I finished building a house and I respond "no the house isn't ready" its not the house fault it's not built it's mine.
jjchmiel78I find it hilarious that ever since Verizon started a major advertising campaign against AT&T, that all AT&T seems to do is shoot themselves in the foot. They need new management yesterday, a new advertising agency now, and really start doing something. Keep shooting themselves in the foot at the pace they have since November and they won't have the business to worry about articles like this in a year or two. iPhone may be cool, but is not the magic wand to let AT&T get away with whatever they want and save their company.
gwellinActully if you pay attention to the AT&T commercials. They say they have coverage to over 98% of the people. That statement says nothing about 3G coverage. They are just saying 98% of people in the US have some cell phone coverage with AT&T. That is why verizon can get away with saying the have over 5x the 3G coverage.
When you have an area that is extremely densely populated, numerically you don't need as many "towers" to provide signal for the area. Plus they can put "towers" on top of buildings, so terrain and landscape aren't issues.
Where as in more rural type cities, hills and mountains seem to get in the way. And since in other cities growth goes outward (as opposed to up and down) you have to provide more towers to cover the area. It would make sense to me if NYC didn't numerically have a lot of towers. They just have towers crammed with transmitters, etc.


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Read an Excerpt
Everyman's Bible Commentary
By Charles C. Ryrie
Moody PublishersCopyright © 1996 Charles C. Ryrie
All rights reserved.
The Superscription, 1:1–3
The Title, 1:1
Although it is true that this book reveals Christ, the genitive expression "of Jesus Christ" means that it is a revelation given by Christ. It is a revelation of "things which must shortly take place." The words translated "shortly" (en tachei) mean that when the time for judgment comes there will be no delay in its execution (see Luke 18:8 and other occurrences of this phrase in Acts 12:7; 22:18; 25:4; Rom. 16:20; Rev. 22:6–7). The time of the fulfillment may seem distant, but, when it starts, the events will transpire rapidly.
The Means Of Communication, 1:1–2
The chain of communication was God the Father, to Christ, to an angel, to John, to God's servants. John, the human instrument, testified of the Word of God (he considered himself in the prophetic succession, transmitting God's message to humanity) and the testimony of Christ (that is, the witness Christ imparts about Himself).
The Value of the Book, 1:3
A blessing is promised the person reading and those hearing and keeping the words of the book. Note the change from singular to plural—one reads and several hear—indicating that the book was to be read publicly. Since public reading was one test of canonicity, the fact that John indicates that it should be read publicly means that he considered it canonical. The entire book is called a prophecy. The phrase "the time is near" is repeated in the epilogue (22:10). "Near" (engus) means "impending or at hand"; these events are near because a thousand years are as a day with the Lord (2 Peter 3:8).
The Salutation, 1:4–8
The Writer, 1:4
The Hebrew idioms in the book, the authority of the author in relation to the churches, the use of distinctively Johannine terms such as logos and "Lamb of God," and the corroboration of Irenaeus, Origen, Tertullian, and Clement all affirm that the apostle John was the author of this book.
The Readers, 1:4
The fact that John specifically addressed the seven local churches in Asia Minor prevents anyone from saying that the book is simply a piece of poetic idealism.
The Greeting, 1:4–5A
This is a greeting from the Trinity. The Father eternally existed and will always exist (the phrase "who is and who was and who is to come" occurs also in 1:8; 4:8; 11:7; 16:5). The "seven Spirits" likely represent the sevenfold ministry of the Spirit as depicted in Isaiah 11:2. Christ is designated as (1) the faithful Witness (summarizing His life on earth, cf. John 7:7; 8:18; 1 Tim. 6:13), (2) the Firstborn from the dead (His resurrection), and (3) the Ruler (not "prince") of the kings of the earth, which refers to His future reign over the earth.
The Dedication, 1:5B–6
The book is dedicated to Christ, who was its Author and about whom it speaks. Three things are ascribed to Him: (1) He loves (present tense) us. (2) He released us (some translations say "washed," the difference in Greek being one letter) from our sins by His blood. Blood is the evidence of His death, which is the basis for our cleansing. (3) He made us a kingdom (not "kings") and priests to God. "Kingdom" views believers corporately and anticipates our association with Christ in His future reign (5:9–10), whereas "priests" sees them individually ministering to our Lord forever.
The Keynote, 1:7
Verse 7 is the text, theme, or keynote of the book and is a reference to Christ's second coming. It is after the Tribulation (note Matt. 24:29–30), it will be public, and all shall see Him and bemoan His crucifixion. This is partly quoted from Zechariah 12:10. "Tribes" is not limited to Israel but includes all the peoples of the earth.
The Authentication, 1:8
Some understand the speaker in verse 8 to be God; others, Christ. It is probably God verifying the contents of this prophecy. Alpha and Omega are the first and last letters of the Greek alphabet, signifying the completeness of God. He is the Lord God, eternally existing, and all-powerful. "Almighty" is used eight times in Revelation (1:8; 4:8; 11:17; 15:3; 16:7, 14; 19:6; 21:22) and includes the ideas of omnipotence and universal rulership.
I "The things which you have seen" (chapter 1)
The Risen Christ
II "The things which are" (chapters 2-3)
The Letters to the Seven Churches
Ephesus · Smyrna · Pergamum · Thyatira Sardis · Philadelphia · Laodicea
III "The things which shall take place after these things" The Tribulation (chapters 4-19)
4. The Throne in Heaven
5. The Scroll in Heaven
7. The Redeemed
10. The Little Scroll
11. The Two Witnesses
13. Antichrist & False Prophet
14. Announcements
15. Prelude to Bowls
17. Religious Babylon
18. Commercial Babylon
19. Second Coming of Christ
The Millennium (chapter 20)
The Eternal State (chapters 21-22)CHAPTER 2
Although there is more than one way to divide the book of Revelation, most commentators see in 1:19 the divinely given outline. In this verse the book is divided into three parts: (1) the things John had seen up to verse 19, (2) the present state of the church (chaps. 2–3), and (3) the things that shall be after the church is completed (chaps. 4–22). The same words translated "after these things" (meta tauta) are found in 4:1, indicating that chapter 4 begins this last section of the book. However, it is possible to combine the first two sections because verse 19 may well be translated, "Write the things which you have seen, both the things which are and ..."
In other words, John saw two things: present things and future things. The present things include the vision of Christ in 1:9–20 and the letters to the churches in chapters 2 and 3. Of course, it does make sense to see 1:9–3:22 as one unified section, simply because in the vision in 1:9–20 Christ is walking in the midst of those churches mentioned in chapters 2 and 3. But it is equally valid to consider the vision of 1:9–20 as that which John had seen and thus as a separate division of the book. The important thing is to notice that according to 1:19 the book has to divide at 4:1, regardless of whether one combines the vision of 1:9–20 with chapters 2 and 3 or divides it into a separate section. Understanding that 4:1 begins the section that describes the future after the times of the churches ends supports a pretribulation Rapture view. In other words, the period of the churches ends at 3:22, and the events of the Tribulation begin to be described at 4:1—"things which must take place [the future Tribulation, return of Christ, and the Millennium] after these things [the completion of the church age]." Posttribulationists who believe that the church will live through the Tribulation on this earth understand 4:1 to indicate only the time when John saw the vision, that is, after he received the letters to the seven churches. But since "after these things" is similar to the phrase in Daniel 2:29, "what will take place," where it clearly refers to the content of Nebuchadnezzar's dream, which concerned the future, then it should be understood in John's use as also referring to the eschatological future.
Circumstances of the Vision, 1:9–l 1
John's Physical Circumstances, 1:9
John does not exalt himself above his fellow believers but calls himself a brother. Patmos is an island about fifteen miles in circumference in the Aegean Sea southwest of Ephesus. The reason for his banishment was because of "the word of God" (God's claims on men) and "the testimony of Jesus" (the gospel message).
John's Spiritual Circumstances, 1:10–11
John's being in the Spirit seems to indicate a trancelike state of spiritual ecstasy. "Was" is literally "became," indicating that this was something unusual. The phrase "the Lord's day" could refer to Sunday or the Day of the Lord, that is, the Tribulation and the Millennium, which are the subject of much of the prophecy. "Lord's" is an adjective (kyriakos), used only here and in 1 Corinthians 11:20 in the New Testament. Outside the New Testament it means "imperial." Unless this is a reference to Sunday, there is no place in the New Testament where this expression is used for that day, since the usual designation is "first day of the week." It could, therefore, refer to that imperial day in the future when Christ will take the reins of earthly government, which is what John saw in his vision. The voice John heard was that of Christ, who is identified as the First and Last in verse 17. All that John saw (cf. 22:8), not just the particular letter to each church in chapters 2–3, was to be communicated to all these seven churches mentioned in verse 11.
The Content of the Vision, 1:12–16
The Position of the Lord, 1:12–13
We have very little knowledge of what our Lord looked like while He was here on earth. He apparently had a beard (Isa. 50:6). There was nothing in His overall personal appearance that would especially attract people to Him (Isa. 53:2), though children liked Him (Matt. 18:2). In this section, however, is a description of how He appears in glory and how we shall see Him some day, for, as John wrote elsewhere, "we shall see Him just as He is," not as He was (1 John 3:2).
The Picture of the Lord, 1:14–16
This picture of the risen, glorified Lord is depicted under a number of similes (note the occurrences of "like" and "as")—the only way He could be described to finite creatures. There are seven features to this picture, and the meaning of these similes may have been left unexplained deliberately in order to convey more than one thing to our minds.
His head, 1:14. It was white as wool or snow. This may represent the wisdom of age and the purity of holiness (Prov. 20:29).
His eyes, 1:14. They were piercing in their fiery holiness. The true character of each church is transparent to His eyes. There may also be a connection between this verse and 1 Corinthians 3:13; that is, the fire that shall try human works at the judgment seat of Christ will be the penetrating gaze of our Lord, which will of itself consume works of wood, hay, and straw.
His feet, 1:15. John's eyes dropped from the Lord's eyes to His feet, which were like burnished bronze. ("Burnished" means "highly rubbed and polished.") This may picture the trials He experienced in His earthly life that make Him a sympathetic High Priest (Heb. 4:15) and an experienced Judge.
His voice, 1:15. It was to John as the sound of many waters. Like the noise of a mighty waterfall, His voice of authority stands out above all the rest and drowns out all who try to talk back or offer excuses to Him.
His right hand, 1:16. In His right hand, the place of honor, were the seven stars, which are explained in verse 20 as the messengers of the churches of chapters 2–3. The word angelos ("angel") means "messenger" and could refer to a supernatural being, implying that each church has its guardian angel. The word could also be used in the nontechnical sense of a human messenger (see James 2:25 and Luke 9:52)—the human leader or pastor of the church.
His mouth, 1:16. The Word of God, the basis for all judgment, proceeded out of His mouth (Heb. 4:12; Rev. 19:12–15).
His countenance, 1:16. Christ's overall appearance was such that it overwhelmed John.
Consequences of the Vision, 1:17–20
A Word of Comfort, 1:17–18
The awesomeness of the vision caused John to prostrate himself before the glorified Christ, waiting for His mercy. This resulted in a threefold word of comfort for the aged apostle. Christ presented Himself as the self-existent, eternal One, "the first and the last"; the Conqueror over death; and the One who controls (by having the keys) Hades (the place that holds the immaterial part of humans after physical death) and death (the experience of the material part).
A Word of Command, 1:19–20
The apostle is then commanded to write the things that he had seen and would yet see. As stated, this forms an outline of the book and is followed in verse 20 by the Lord's own explanation of two features of the vision. The "stars" are the angels (either guardian angels or the human leaders) of the churches, and the "lampstands" represent light-bearing local churches.CHAPTER 3
The seven churches addressed by letter in chapters 2 and 3 are significant in several ways. First of all, at the time John wrote they were actual churches that existed in the cities mentioned. They were not necessarily the most prominent ones of that day, since only two—Ephesus and Laodicea—are previously mentioned in the Bible. But they were actual churches with problems and strengths recorded of them. This means, of course, that, just as there was an Ephesian church in John's day, there was also a Laodicean church in the first century.
These churches were representative of all churches at that time, as well as those of subsequent generations. Just as the letters to the Corinthians, though written to the church at Corinth, concern the church everywhere and at all times (1 Cor. 1:2), so these letters are for the church past, present, and future.
Two reasons substantiate the representative character of these seven churches. The first is simply that there are only seven selected. Out of all the churches that might have been chosen (though not all in Asia Minor), such as Jerusalem, Antioch, Alexandria, Corinth, Rome, Troas, Colossae, or Hierapolis, only seven are selected. Second, in the promise to each of these churches at the close of each letter is the exhortation to hear what the Spirit says to "the churches." Though each letter is written to a church, the exhortations are to all the churches.
Some see a third significance of these churches, regarding them as representing seven successive periods throughout church history. One writer says, "The varying conditions represented in these seven churches in order of their succession fit uniquely into the checkered history of the church universal from start to finish." This idea does not deny their local and representative character, but it often tends to obscure their local and representative importance by placing so much emphasis on the Philadelphian or Laodicean church as the era in which the church is presently living. We forget that there are many local churches today with, for example, Ephesian and Pergamum characteristics.
Each letter is addressed to the angel of the particular church. The word "angel," as previously discussed, can refer to either a superhuman or human being. If angel refers to the human leader of each church, then that leader's responsibility for the condition of the church is emphasized in the address of each letter: "to the angel of the church ..."
These letters comprise the "things which are." They depict conditions that did and do exist in the churches, then and now. Each letter may be outlined under six headings: Destination, The Lord, Commendation, Condemnation, Exhortation, and Promise.
The Letter to Ephesus, 2:1–7
Destination, 2:1
Ephesus was the capital of the province of Asia and the residence of John before and after his exile on Patmos. A city of about 300,000, it was a commercial center with warehouses that lined the banks of the Cayster River. It was on a principal east-west road. Pan-Ionian games were held there in May. It was known throughout the world as the home of one of the seven wonders of the ancient world—the temple of Diana. This magnificent structure stood in an area 425 by 220 feet, with 126 pillars of marble 60 feet high. Thirty-six of these were overlaid with gold and jewels. Eunuchs and slaves attended the temple, and the city proudly entitled itself the guardian of the temple and statue of Diana (Acts 19:35).
Excerpted from Revelation by Charles C. Ryrie. Copyright © 1996 Charles C. Ryrie. Excerpted by permission of Moody Publishers.
All rights reserved. No part of this excerpt may be reproduced or reprinted without permission in writing from the publisher.
Excerpts are provided by Dial-A-Book Inc. solely for the personal use of visitors to this web site.


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The 2013 U.S. Open Championship is only two days in, but some household names have already dug themselves a hole.
Those holes aren't impossible to climb out of, especially for the former U.S. Open champs who find themselves buried. However, the stars who are already in catch-up mode haven't shown anything in this or other recent major tournaments to lead one to believe that they'll bounce back strong enough to leapfrog the leader by Round 4's conclusion.
Here are three players who entered the weekend as favorites, but will exit disappointments.


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I am the owner of Knitspot - knitting patterns, clubs, and artisan yarns. I am a life-long knitter with a background in the fashion and graphic design fields, and began designing clothing, knitwear ...
I am the owner of Knitspot - knitting patterns, clubs, and artisan yarns. I am a life-long knitter with a background in the fashion and graphic design fields, and began designing clothing, knitwear and accessories during the 1970s. I teach and write about knitting, spinning, and designing at my blog, Knitspot. I live and work in Ohio with David, who loves wool, too.
My design work/profile has been included in Knitty, The Knitter, Interweave Knits, and Twist Collective, Sock Club, Brave New Knits, as well as several upcoming publications. The complete catalog of Knitspot patterns is available for browsing and purchasing at http://www.knitspot.com/knitting_pattern/index.php
Knitspot yarn clubs are reknowned for perfect pairings of luxury yarn and design, as well as the rollicking good fun in our Ravelry clubhouse where members meet, chat, and knit through club projects together.
Our Bare Naked yarns are as close to the farm as a knitter can get, using fresh fiber produce from regional growers which is then spun into yarn at smaller artisan mills with which I have worked over the years. The collection is focused on single-breed pure wool yarns and exotic blends, with colors that range an entire array of natural tones—yarn the way nature made it.
A scarf or neckwarmer in two sizes (S/L) to match the Eliza mitts with the same pleated hem flounce and pretty snowdrop lace. The neckwarmer features a button side closure. Scarf is worked in two pieces and grafted at center back. An excellent first lace project that experienced knitters will enjoy as well for its suitability as a quick knit gift project. Substituting yarn and/or needles may result in an overall variation in size.
30 sts and 44 rows = 4 inches in stockinette stitch on smaller needles (unblocked)


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Fits Hot Rod Deluxe, Prosonic Combo. Rugged black vinyl. Amplifier dimensions: Height: 18.75" Width: 23.5" Depth: 10.5"
Reviewed by 3 customers
Comments about Fender Hot Rod Deluxe Amplifier Cover:
I had trouble finding an amp cover for my Fender Princeton Chorus. Since the product description included dimensions, it appeared this Hot Rod cover would work. I received the cover in three days and it fit perfectly. Quality is excellent and is easily cleaned with a damp cloth. Great product...great service.
Comments about Fender Hot Rod Deluxe Amplifier Cover:
Quality material and perfect fit. Don't leave home without it!
Comments about Fender Hot Rod Deluxe Amplifier Cover:
Firstly, I would like to say that the Hot Rod Deluxe is an amazing amp. And an amazing amp deserves to be well taken care of. This cover came with my Deluxe and I use it whenever I'm not actually playing the amp to protect it from dust, cats, little brothers, rain, and just about everything else that threatens the well-being of my amp. The handle hole is a good idea and the material is of good quality. If you have a Deluxe and want to make sure it stays in good shape for a long time, get this cover.
Our product catalog varies by country due to manufacturer restrictions. If you change the Ship-To country, some or all of the items in your cart may not ship to the new destination.


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As the NBA Trading Deadline finally comes to a close, Celtics fans did not see a major move that many believe they needed to make the playoffs, but they did acquire the three time Slam Dunk Contest Winner, Nate Robinson from the New York Knicks.
The five player deal between New York and Boston went as followed:
Celtics Receive:
G Nate Robinson
SF Marcus Laundry
Knicks Receive:
G Eddie House
SG J.R. Giddens
GF Bill Walker
The deal is expected to be made official soon and many will speculate as to whether or not the Celtics did enough.
The Celtics needed to get younger. The "Big Three" have been showing that they are not getting any younger, and whilst acquiring Nate Robinson provides some youth off the bench, I wonder as to whether or not that is enough to propel the Celtics to the playoffs…let alone survive if they get there.
Robinson is good, no doubt there.
He is 25 years old and scores about 13 PPG. He'll perform well in the position given to him in Boston, but this just was not enough, and it heightens our dependency on the aging "Big Three."
Allen, Garnett, and Pierce must stay healthy and play well consistently if they want to reach the playoffs. They can do it, but it will be a long and hard road if those three are not performing well.
Nate Robinson's role is to be the difference maker on the bench and mature under the wings of leaders such as Kevin Garnett and Paul Pierce. This move will benefit Robinson, but the impact he makes for the Celtics will not be big enough to win a Championship.
It is now up to Allen, Pierce, and Garnett, alongside an inconsistent bench, to step up their game and rebound from a very disappointing first half. They can do it, but it will be a tough road ahead.
First reported at Beantown Sports Observer


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HS athletes stay safe practicing in summer heatUPDATED 3:44 PM CDT Jul 12, 2013
Doctors advise athlete to stay hydrated and keep safe while practicing outside in the hot summer months.
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Operations on the Conrail bridge in Paulsboro resumed Sunday, the same day workers removed the last of four derailed tanker cars from the Mantua Creek.
The Federal Railroad Administration conducted two test runs and completed its inspection of the bridge Sunday afternoon, an FRA spokesman said Monday.
Conrail declined to comment on what damage the bridge had sustained in the derailment and how it was repaired so quickly, citing an investigation by the National Transportation Safety Board. NTSB spokesman Peter Knudson said the federal agency expected to complete its investigation of the accident in the next 12 to 18 months.


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Progressive Cinema: Toronto International Film Festival 2009, Part 4
Among the more than 350 films shown this year at the Toronto International Film Festival, several are of importance to a progressive audience. In addition to titles mentioned in previous columns ("The Time That Remains, "Capitalism: A Love Story," "The Informant!" "Men Who Stare at Goats" and others), the following are just a few of the many at the festival that draw interest for progressive filmgoers.
Of course one of the most famous political activists in American history, Daniel Ellsberg, receives his very own personal documentary, "The Most Dangerous Man in America." This is the tag Richard Nixon affixed on Ellsberg when he exposed the Pentagon Papers. The film recounts the events in detail and is a thoroughly engrossing account of how Ellsberg enlisted his friends (and son) to photocopy thousands of pages of classified Pentagon documents. His actions certainly shortened the war in Vietnam and Ellsberg went on to support antiwar causes to this day. He and his wife, Patricia, received a five-minute standing ovation from the sold-out Canadian showing.
Another likely box office hit shown in Toronto was "Creation," a British drama centered around the time in Charles Darwin's life when his 10-year-old daughter died of a tragic illness. Darwin and his God-fearing wife, played by real-life couple Paul Bettany and Jennifer Connelly, struggle with differences around religion, at a time when Charles is writing his "Origin of the Species" and developing a more secular humanist approach. The plot centers mostly around his struggle writing while his daughter is suffering from her illness. Friends and associates encourage him to continue writing what they feel will be a groundbreaking study of evolution. It changed the world. The acting, sets and music are stunning, and you can certainly say it's the best drama ever made about Charles Darwin. There are no others.
A Chinese film, "City of Life and Death," deals with the relatively unknown 1937 Japanese attack on the then Chinese capital, Nanking. It is controversial in that Japan denies the death toll count of hundreds of thousands of civilians, and the reported rape of 20,000-80,000 women during the surprise attack and six-week-long brutal occupation. The black and white Chinese production is awesome and epic in scope, utilizing thousands of extras and extensive special effects. The acting, sets and music are of topnotch quality, far surpassing, as the recent Chinese Olympic festivities showed, audience expectations. An amazing antiwar film! (See mediaasia.com/cld/eng.)
Another historical drama, "The Front Line," examines the Italian extreme left terrorist group that formed in the '70s patterned after the Red Brigade but more violent and extreme. What starts out as idealist youth angered by corporate power and exploitation leads to bombings and assassinations, and an eventual and tragic disconnect with the masses they felt they were fighting for. The film follows the core leaders and their advancing destructive tactics which led to imprisonment for all of them that survived. The film separates itself from others by its compassion for all the subjects on both sides of the struggle. There is a real attempt to understand motivation, responsibility and commitment to a cause. The non-stop action and growing tension among the members of the failing faction make the film engrossing and rewarding cinematic fare.
And one more well known historical event is addressed in the film "Jean Charles." After the 2005 London subway bombings, a young Brazilian man was tracked down and shot as a suspect in the alleged terrorist plot. The film follows his arrival in London and experiences in finding employment and housing. Jean Charles became well liked in his community and was just making headway when he was fatally shot by the police. The rest of the film follows his friend's attempts to prove his innocence and find consolation for his Brazilian family.
Another of the many titles that warrant viewing is "Schmatta: Rags to Riches to Rags," by Marc Levin, director of "CIA: America's Secret Warriors" and "Slam." "Schmatta" beautifully tells the story of the New York garment industry and the workers and unions that kept it going. Loaded with political awareness, the film is a testament to the working class and its rich history.
And finally, to briefly mention a few more films that should be of interest to progressive viewers: "Max Manus," the epic adventure film about the famed Norwegian World War II resistance fighter; "Moloch Tropical," a Haitian allegory on the abuse of power, by Raoul Peck (who also did "Lumumba"); and "Balibo," which relates the chilling murder of five Australian journalists during the Indonesian invasion of East Timor.


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Electric Power Research Institute
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The Electric Power Research Institute, or EPRI , conducts research on issues related to the electric power industry.
EPRI is a nonprofit organization funded by the electric utility industry, founded in 1972 and headquartered in Palo Alto, California.[1] EPRI is primarily a US-based organization, but receives international participation. EPRI's research covers different aspects of electric power generation, delivery and its use.
Following the Northeast blackout of 1965, the Senate held hearings in the early 1970s about the lack of research supporting the power industry.[2] All sectors of the U.S. electricity industry pooled their funds to begin an industry-wide collaborative research program.[citation needed] EPRI was established, as a response, in 1972 as the Electric Power Research Institute. Created as an independent, nonprofit organization designed to manage a broad public-private collaborative research program on behalf of the electric utility industry, the industry's customers, and society at large. EPRI's creation was a recognition of the impact of electricity on modern life.
The institute's research and development program spans every aspect of generation, environmental protection, power delivery, retail use, and power markets. EPRI provides services to more than 1000 energy-related organizations in 40 countries. It has more than 900 patents to its credit.[3][citation needed]
EPRI laid the groundwork in the 1970s for the use of power electronics in the utility system, sometimes known as FACTS (Flexible AC Transmission Systems), established the largest electric and magnetic fields health program in the world and has played a role in resolving scientific questions concerning potential links to cancer.[citation needed] EPRI is in the Advisory Council of the PHEV Research Center and created the world's largest center for nondestructive testing, used first for nuclear inspection and now increasingly for internal diagnostics of fossil power plants and industrial systems.[citation needed]
Chauncey Starr was the founding president of EPRI.
Renewable energy[edit]
According to Revis W. James, the former director of the Energy Technology Assessment Center at EPRI, renewables such as wind and biomass are predicted to supply only a quarter of the electricity in 2050. He also predicted that solar power will not play a significant role. "It just doesn't enter into our equation," said James in an April 2009 article in The New York Times Energy and Environment Blog. As of 2011, James is the director of Generation Research and Development.
As defined in EPRI's mission, research is conducted with the intention of improving the current state of the energy industry.[4]
Coal-fired energy[edit]
In 2010, EPRI was part of a team to be awarded with Power Engineering magazine's 2010 Coal-Fired Project of the Year. The project, "DryFining," created a new technology for coal-firing power plants that improves fuel quality, decreases volatile gas emissions, and reduces a plant's operating expenses and maintenance costs. The team was led by electric service provider Great River Energy of Maple Grove, Minnesota, and also included fluid bed dryer engineer Heyl & Patterson Inc. of Pittsburgh, Pennsylvania, the U.S. Department of Energy's National Energy Technology Laboratory, Lehigh University's Energy Research Center and engineering construction contractor WorleyParsons.[5]
See also[edit]
- ^ "Electric Power Research Institute, Inc.: Private Company Information - Businessweek". www.bloomberg.com. Retrieved 2016-08-05.
- ^ http://mydocs.epri.com/docs/CorporateDocuments/EPRI_Journal/2012-Summer/1025689_Blackout.pdf
- ^ http://www.epri.com/search/Pages/results.aspx?k=patents&r=mptabresults%3AARABUmVzZWFyY2ggUmVzdWx0cwxtcHRhYnJlc3VsdHMBAl4iAiIk,mplevel1name%3AAQpHZW5lcmF0aW9uDG1wbGV2ZWwxbmFtZQEBXgEk
- ^ http://www.epri.com/About-Us/Pages/Our-Business.aspx
- ^ Morris, Lindsay. "Power Engineering Names Projects of the Year". Power-Gen Worldwide. Pennwell Corporation. Retrieved 2010-12-14.
External links[edit]
- Energy research institutes
- Engineering research institutes
- Electric power in the United States
- Energy in California
- Research institutes in the San Francisco Bay Area
- Research and development organizations
- Non-profit organizations based in California
- Organizations based in Palo Alto, California
- 1973 establishments in California
- Research institutes established in 1973


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- Uttarakhand
- Tourism
- Hotels
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- Culture
- Education
- News
- Villages
- About Us
- Contact
Patal Bhuvaneshwar is a limestone cave temple 14 km from Gangolihat in the Pithoragarh district of Uttarakhand state in India. According to legends and folklores, this underground cave enshrines stone carved idols of Lord Shiva and 33 koti (33 crore) type of gods in hindu culture. There are few hotels at Patal Bhuvaneshwar but all of them are pretty good and provides a comfortable stay with all the modern facilities. You can also find Hotels at Chaukori which is just 42 kms from Patal Bhuvaneshwar.
Book online Patal Bhuvaneshwar hotels and resorts and get exclusive discounts on hotel room rates and packages.


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Ninjatrader MACD to MarketAnalyzer
This project was successfully completed by glivv for $75 USD in 3 days.Get free quotes for a project like this
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Project Budget$50 USD
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Another projekt I would like you to look at and say if you can do this.
I need to now your skills for MarketAnalyzer and similar counter indicator on picture "[url removed, login to view]" number count is wrong, only a picture.
I want to specify up to 30 numbers like 5,8,13 and so on like a simpel counter for candles. Starting point for count is from every pink dot. I have this indicator there make pink dots. this numbers can be stacked on each candles up to 30 numbers depending on how many numbers I want to count on. I would like to have this stacked numbers from the lates candels in to MarketAnalyzer cells if it's possible.?
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America's Sweetheart
The Cat With The Cream
Heart Of A Dog
Third Eye - The Odyssey - Chapter 7
Surprise Yourself
Anytime (Live on Letterman)
Come To Daddy
What I Did For Love feat. Emeli Sandé [Lyric]
The film is part of a new DLF project, 'Playing Lynch'.
New characters, new inspiration and new themes.
One Marvel Universe star interviewed another, as part of Interview magazine's October edition.
A sharp script and especially colourful imagery make this animated romp a lot more fun...
A solid cast bodes well for this unnecessary remake of the 1982 movie (based on...
The overly-confident alien race, the Boov, have faced some difficulties in their time, but they've...
Comedies don't get much more self-referential than this film, in which a bunch of old...
When actor James Franco threw a party for his fellow movie stars including the star-studded...
You'd have to go back to 1998's Armageddon to find another film that so adeptly...
The ocean is vast. Many parts of it are unexplored by man; those parts make...


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Star Tracks: Monday, May 16, 2016 42 years, 2,191 covers and 55,436 stories from PEOPLE magazine's history for you to enjoy
Latest News!
- Jennifer Aniston and Justin Theroux Step Out for a Date Night in N.Y.C.
- Read the Cover Story: Brad & Angelina Split After 12 Years: It's Over
- Country Singer Cam Marries in Intimate Desert Ceremony: All the Details
- Presenting… Every Single Thing the Kardashians Have Said About Their Nipples
- WATCH: The Bachelor's Chris Harrison on His Love Life: 'I Truly Couldn't Be Happier'
People Top 5
LAST UPDATE: Tuesday February 10, 2015 01:10PM EST
PEOPLE Top 5 are the most-viewed stories on the site over the past three days, updated every 60 minutes
- June 12, 1995
- Vol. 43
- No. 23
Rhapsody in Blue
For Washington's Doo Wop Cops, Singing Isn't Just a Night Shtick—it's a Way of Teaching Kids to Play Safe
They call themselves D.C.'s Finest, but they are better known as the Doo Wop Cops, a group of retired and active Washington-area police officers who perform at nursing homes, hospitals and especially inner-city schools, where their mission is not just to entertain but also to promote responsibility—and survival. "The guns and the danger are out there every day," says baritone Deane Larkins, 48, an active-duty patrolman. "This is the worst I've ever seen, because of the drugs and the mentality of the kids, who have no respect for the law or for anything." In hopes of reversing that trend, they've come to Blow as part of A Time for Peace, a program at the school that tries to get kids to resolve differences nonviolently in a neighborhood where disputes often end in gunfire. "It sounds like a wonderful program," retired Det. Jimi Bethel, 48, the group's bass and manager, booms to the kids. "But who can tell me about it?"
A little girl in a pink sweat suit raises her hand, then gets too shy to say anything. Six-year-old Tyrina Lee takes a shot. "We don't talk to bullies, and we don't smoke," she says, to huge applause from the other children. "And we don't fight with people that can hurt us," adds 4-year-old Maurice Primrose. After another round of applause, the Doo Wop Cops step back into musical character, belting out the classic hit "Blue Moon."
Bethel and Larkins and their fellow Finests—retired Det. Ron Jones, 49, and Reamer "Junebug" Shedrick, 47, a member of Mayor Marion Barry's security detail—were old friends who began making music together 10 years ago. Retired dispatcher Rich Collins, 49, joined the group in 1990. "We'd run into each other, and sometimes we'd just step down the hall and get to singing," says Jones. "It was so much fun."
After performing at the wedding of a fellow police officer in 1985, they went on to win a local talent show, then took their act on the road—including to the Apollo Theater in New York City—and to the streets. Billing themselves as entertainers, role models and counselors, the Doo Wops perform mostly for free. (In September 1993 they sang at Gen. Colin Powell's retirement party, where guests included the Clintons and the Bushes.)
Part of their strategy is to show that police "can do something other than lock people up," says Jones, whose 22-year-old son, Ron Jr., was shot dead in a traffic dispute in 1990. When the Doo Wop Cops finish up with Hard to Say Goodbye, they always dedicate it to the memory of Roy Jr. To prevent such loss, "we need thousands of guys like us to talk to the kids," says Bethel. "Because you'd be surprised, so many of them do want to listen."
KATE McKENNA in Washington
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Shamus Cooke: The pathetic state of democracy in Egypt led the spokesman for the military's candidate, Ahmed Shafiq, to declare "the revolution has ended."
Austerity Measures Meet Resistance and the Democratic Will
Carl Bloice: It's funny how a dramatic political crisis can focus the mind, how things like the Occupy movement and the European voters' revolt can shift perception — even the public expression — of the powers-that-be in politics and the media.
What European Elections Should Teach Obama
Randy Shaw: Europe's lesson is that Obama should far more aggressively promote public investment, job creation, and other economically populist – and popular – measures.
25 Million Unemployed: Why So Little Protest?
Sherwood Ross: Although America's 25 million unemployed and underemployed could be a powerful force for social change, they aren't combining in any effective way to protest, an eminent business authority writes.
Elected on Hope, Obama Governs Through Fear
Randy Shaw: Obama ran for president preaching hope and rejecting appeals to fear, but now has become exactly what he railed against.
Is Barack Obama's Realism Better than George W. Bush's Idealism?
Barack Obama's reaction to the mass protests and violence in Iran shows he is following through on his pledge to be more like George H.W. Bush rather than his son, George W. Bush. Obama has admired the father's realism and has criticized the idealistic neo-conservatism of the son. But is realism a better foreign policy […]


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Civilization 5: Gods and Kings Cheats
5ZIGN posted on Jul 09, 2013 1:47:13 AM - Report post
Hey P, Caliber, and crew, Here is a donation from me, to show my appreciation for all u guys hard work and so we could keep this civ5 active and I also hope u guys will release a trainer for civ5 a brave new world as well for those who love the civ5 series. I know that u guys are pretty frustrated with all the complains and problems. I'm a gamer and I love pc games. I became an unlimited member because I need Trainers to beat certain levels or just for the fun of it. and the unlimited member is well worth it. Some people just need to see what's on this website: How many games there is From A to Z, and how many trainers has been release upon updates after updates. That speak for itself, That's a lot of hard work. So those people need to show a little respect and appreciations. U guys can only do so much... And thanks to those who have made a donation to support. So that we can all (including those who whines) continue and benefit from P, cal and crews for their hard work.
PWizard posted on Jul 09, 2013 7:32:30 AM - Report post
Thanks very much!
Chris O'Rorke (firstname.lastname@example.com)
Co-Founder: Cheat Happens.com
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