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16 years of experience
1516 Garcia Ave
Miami, FL 33146 Phone number & directions
A medical license is required for a doctor to practice medicine in a particular state. Requirements vary by state but most require, at a minimum, post-graduate training in the doctor's specific specialty. An NPI number is a national identifier unique to an individual health care provider and is managed by the Center for Medicare & Medicaid Services (CMS).
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Specialties & Research
Dr. Allan Hernandez, MD is board certified in Family Medicine.
Education & Awards
Dr. Hernandez graduated from Universidad Central Del Este (Uce), San Pedro De Macoris.
Dr. Hernandez has 3 hospital affiliations:
- Memorial Regional Hospital South
- Hialeah Hospital
- Pan American Hospital
- Spanish
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- Dr. Allan A Hernandez MD is a male Family Practitioner, has 16 years of experience and practices in Family Medicine.
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Dr. Allan Hernandez practices Family Medicine near Miami, FL.
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Created in this Craftsy class
Jewelry Workshop: Bead, Wrap, Chain & Etch taught by Max Goodman
Build a broad skill base for getting started making beautiful, timeless jewelry. This is the perfect class for beginners to test many different types of techniques, from beading to wire wrapping and beyond.
Other projects made as part of this class:
JewelryPearl Knotting Technique
JewelryFlower Bracelet
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Llove it and Llove her
08/22/2012 Flag
lol I like the chain and that you love yourself is a sign of greatness in its self. :) good job.
09/16/2012 Flag
Thanks! It s fanny, my husband wrote that! I
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Very nice. Love it!
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Me encanta, Andrea!
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I hold a MSc in applied Parasitology from Kenyatta University, Nairobi, Kenya. I have published Seven research articles in public health-related fields since 2009. I am a peer reviewer of several research Journals including Science Alert, Academic, Journal of Vector Borne Diseases, etc. I have a vast experience spanning over 25 years in the control, prevention and research on vector borne and parasitic diseases, and medical and veterinary entomology in general. My research work was carried out in the Kenya Medical Research Institute. My...Read More »
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Our view: Sequester cuts will hurt Erie
One month after Congress gave up on its responsibility to agree to a budget compromise, the real effects of sequestration are starting to sink in.
Automatic spending cuts of $85 billion, hitting both domestic and military spending, went into effect on March 1. As President Barack Obama and members of Congress wrangled about who would get the blame (or possibly even the credit) for the drastic spending cuts, sequestration seemed like a bureaucratic exercise confined to the Beltway.
But federal dollars are important to regular people, including Erie County residents. Shari Gross, director of the Erie County Department of Human Services, estimates that the county will lose between $1.9 million and $2.7 million in federal funding during the 2013-14 fiscal year because of the sequester cuts. Erie County and local service providers "can't make up $2 million or more" on their own, Gross warns. "The money's not there. You're going to end up cutting programs, providing less services," Gross says.
The sequester cuts are the norm for now. On Wednesday, Obama signed legislation to fund the government through September to avoid a government shutdown. "The legislation locks in the automatic spending cuts that took effect on March 1 and denies a scheduled 0.5 percent pay raise for federal workers," the Federal Eye blog at the Washington Post reported.
Many American workers who have taken cuts in pay and benefits and who have cut back to live within their means won't get up in arms because federal workers face a pay freeze and because agencies must cut hours and furlough workers.
But federal dollars also pay for programs and services that are vital to Erie County residents. Parents and employers will feel the pinch when subsidized day care is reduced. Troubled people will be hurt when there is less money for mental-health counseling. Individuals struggling with substance abuse will face serious challenges when there are less funds for drug and alcohol counseling.
In a March 15 Guest Voice column, Joan Benso, president of Pennsylvania Partnerships for Children, said 2,300 Pennsylvania children will lose access to Head Start and Early Head Start due to the sequester. "Take away funding for early learning, and you've robbed those children of a once-in-a-lifetime opportunity to create a solid foundation for success. And the price for that fiscal neglect doesn't just hit those children and their families. We all pay the price in the form of a less competitive workforce because we failed to invest in our greatest economic resource, our children," she wrote. In Erie County, quality early childhood education provides a way out of poverty and a path to self-sufficiency. Human services programs are designed to do the same.
How many Erie people will be hurt before Congress buckles down in the Beltway and starts to work together to strike a budget deal?


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Linguica, Kale, and Potato Soup
Total Time
Prep 40 mins
Cook 30 mins
This tasty recipe is similar to one I saw on The Food Network. This soup is full of texture, and is not pureed like a traditional Caldo Verde. I often use "no salt added" beans and tomatoes, reduced sodium broth, and rarely need to add extra salt at all, but other folks may want more. We like it with a little sour cream, along with a head of roasted garlic and a hearty loaf of good bread.
Ingredients Nutrition
- 2 tablespoons extra virgin olive oil
- 3 medium waxy potatoes, peeled & diced (Yukon Golds, white or red potatoes all work well)
- 2 medium onions, chopped
- 4 -6 cloves garlic, minced
- 2 whole bay leaves
- 1 lb kale, chopped center tough stems removed (approx)
- 1 (14 ounce) can garbanzo beans, drained & rinsed
- 1 (14 ounce) can diced tomatoes, undrained
- 1 lb linguica sausage, diced
- 1 quart chicken stock
- fresh ground pepper
- kosher salt
- 6 tablespoons sour cream (optional)
- Heat olive oil in a deep, heavy pot over medium high heat.
- Add potatoes and onions, cover and cook for 5 minutes, stirring occasionally.
- Add garlic, bay leaves, and kale.
- Cover and wilt greens about two minutes; season with salt (if desired) and pepper.
- Add beans, tomatoes, linguica,& broth and bring to a full boil.
- Reduce heat to medium, cover and cook 15 minutes or until potatoes are tender.
- Remove bay leaves; serve soup with a dollop of sour cream (optional) and some of your favorite crusty bread.
Most Helpful
Great mixture of flavors! I liked that it was a hearty potato-based soup, but not a creamy soup. This gave the kitchen a wonderful scent. We're mostly vegetarians here, so I didn't use any sausage, just added some kick with smoked paprika and Penzy's Mural of Spices mix and lots of pepper. That worked out just fine. Also used navy beans, as I didn't have garbanzos, and they added a good creaminess. I thought the liquid amount was fine. I had the soup on the stove 20-25 min. at the end. Great New year's soup!
This soup was delicious! I added Swiss chard since I had it in my garden instead of the kale. I thought the quart of chicken stock was sufficient since my husband and I enjoy a thicker soup. We also added a teaspoon of sour cream as suggested to each cup of soup and it really added a nice tang. Great for a football Sunday evening dinner!
I made this soup and it was wonderful!! However, I felt it needed 2 quarts of stock and that worked out well. I also used kielbasa instead of linguica. Very simple, delicious, a keeper.


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22 July 2016
I made up my sandwich with a normal sized bread. It was a bit of a problem getting the sandwich into the bag, but got there. I put it into the toaster, but then I had to switch off the toaster as it was beginning to smoke! I then removed the 'toastie' from my toaster, only to find that I had to cut the toastbag to get the toastie out of the bag. Sorry, but I think this has to be one of the worst items that you are selling @ the moment. I will return the other toastie bag to you if you send me a prepaid envelope. Thank you.
A note from the team
Thanks for taking the time to give us your feedback. I'm sorry that this hadn't worked as well as expected. I'll be in touch shortly.


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Thursday, June 14, 2012
Summerville Town Council agreed Wednesday to make some basic changes to the employee handbook, but left heftier questions for the budget retreat later this summer.
Director of Administrative Services Lisa Wallace said the changes were to provide a more detailed policy on light duty and to change outdated wording that refers to a town administrator.
Summerville hasnt had a town administrator since Dennis Pieper resigned more than a year ago. After Mayor Bill Collins took office, the council agreed to allow him to take on some of the duties that an administrator would do.
Councilman Bob Jackson initially wanted to table the changes until council could hash out a policy on rehiring retirees, but he agreed that discussion could wait for the budget retreat.
Councilman Aaron Brown also asked that the handbook change from saying employee evaluations may be conducted to will be conducted.
What Im trying to get away from is subjectivity in promoting employees, he said.
Having evaluations on file provides a history of employee performance, he said.
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The Movie Buff Test
Their result for The Movie Buff Test ...
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They scored 76% on moviepoints, higher than 71% of your peers.
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Radio is Your Forte!
you did ok, but you need to get out there and Watch some movies. or maybe just stick with the Radio! Read more
Your up on your movie info, if you didn't use google message me and lets talk! Read more
OMG! you aced it! good for you, message me and lets talk movies if you didnt google your way through the whole thing =D Read more
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Fewer homeowners are falling behind on their mortgage and a record number of homeowners are taking advantage of record low interest rates, according to one credit reporting agency.
Late payments have dropped to a three-year low in the second quarter, with only 5.49 percent of families behind on their payments by 60 days or more.
The numbers come from TransUnion, fro the period between April and June.
Mortgage delinquencies in America were running less than two percent before the housing market collapsed.
At the same time, homeowners are taking advantage of record low interest rates and refinancing in record numbers. Refinance applications for government insured loans soared nearly 200 percent from May to June, reaching a three-year high.
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The "Trump train" was derailed in Wisconsin as voters in the Badger State gave Ted Cruz a major victory over "The Donald." The big question now is – are the Republicans headed for a contested convention this summer?


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The host response to dengue virus infection is characterized by the production of numerous cytokines, but the overall picture appears to be complex. It has been suggested that a balance may be involved between protective and pathologic immune responses. This study aimed to define differential immune responses in association with clinical outcomes by gene expression profiling of a selected panel of inflammatory genes in whole blood samples from children with severe dengue infections.
Methodology/Principal Findings
Whole blood mRNA from 56 Indonesian children with severe dengue virus infections was analyzed during early admission and at day −1, 0, 1, and 5–8 after defervescence. Levels were related to baseline levels collected at a 1-month follow-up visit. Processing of mRNA was performed in a single reaction by multiplex ligation-dependent probe amplification, measuring mRNA levels from genes encoding 36 inflammatory proteins and 14 Toll-like receptor (TLR)-associated molecules. The inflammatory gene profiles showed up-regulation during infection of eight genes, including IFNG and IL12A, which indicated an antiviral response. On the contrary, genes associated with the nuclear factor (NF)-κB pathway were down-regulated, including NFKB1, NFKB2, TNFR1, IL1B, IL8, and TNFA. Many of these NF-κB pathway–related genes, but not IFNG or IL12A, correlated with adverse clinical events such as development of pleural effusion and hemorrhagic manifestations. The TLR profile showed that TLRs were differentially activated during severe dengue infections: increased expression of TLR7 and TLR4R3 was found together with a decreased expression of TLR1, TLR2, TLR4R4, and TLR4 co-factor CD14.
These data show that different immunological pathways are differently expressed and associated with different clinical outcomes in children with severe dengue infections.
Author Summary
Dengue virus infection is an impressively emerging disease that can be fatal in severe cases. It is not precisely clear why some patients progress to severe disease whereas most patients only suffer from a mild infection. In severe disease, a "cytokine storm" is induced, which indicates the release of a great number of inflammatory mediators ("cytokines"). Evidence suggested that a balance could be involved between protective and pathologic cytokine release patterns. We studied this concept in a cohort of Indonesian children with severe dengue disease using a gene expression profiling method.
The study showed that the immune response to severe dengue infection was characterized by up-regulation of interferon pathway–associated cytokines and down-regulation of nuclear factor (NF)-kappaB pathway–associated cytokines. Many of the NF-kappaB pathway–related genes, but not the interferon pathway–associated genes, correlated with adverse clinical events such as development of pleural effusion and hemorrhagic manifestations. In addition, the study showed that the expression of specific pathogen recognition receptors called Toll-like receptors was differentially regulated in patients with dengue. Together, the data show that different immunological pathways may indeed be differently expressed and associated with different clinical outcomes in children with severe dengue infections.
Citation: de Kruif MD, Setiati TE, Mairuhu ATA, Koraka P, Aberson HA, Spek CA, et al. (2008) Differential Gene Expression Changes in Children with Severe Dengue Virus Infections. PLoS Negl Trop Dis 2(4): e215. doi:10.1371/journal.pntd.0000215
Editor: Maria Guzman, Instituto de Medicina Tropical Pedro Kouri, Cuba
Received: June 20, 2007; Accepted: February 19, 2008; Published: April 9, 2008
Copyright: © 2008 de Kruif et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by a grant from the Royal Netherlands Academy of Arts and Sciences (project number 99-MED-04). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Dengue disease is emerging in the developing world at an alarming rate [1]. Moreover, the disease is potentially lethal, but only supportive measures are available. A major obstacle in the development of novel therapeutic strategies is that the pathophysiology of dengue disease is poorly understood [2],[3]. Clinically, dengue disease is a mosquito-borne disease especially affecting children in endemic, mostly tropical regions and is caused by infection with dengue virus, a member of the Flaviviridae family [4]. Though most patients only suffer from a mild febrile illness called dengue fever (DF), a relatively small group of patients experiences more severe forms of disease which are characterized by increased vascular permeability leading to bleeding manifestations and shock, such as dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) [5].
The mechanism leading to severe, critical disease is insufficiently understood. Antibody-dependent enhancement of viral replication is generally thought to play a role since most cases of DHF/DSS occur after secondary infection by a dengue virus serotype different from the first infection [2], [6]–[8]. Severe disease is characterized by high viremia titers, coagulation abnormalities and activation of the immune system [5],[9]. The host defense strongly depends on interferon (IFN)-γ production, but other cytokines are also involved, as well as antibody production and T cell responses [10],[11]. Typically, cytokine levels are highest among the day of defervescence, which is defined as the first day after the start of fever that the body temperature returns to normal. At the same day viremia titers drop to low or undetectable levels, whereas the risk of shock development is increased [12]. The relationship between cytokine production and severe disease is not fully understood, but it is increasingly recognized that a balance is involved between protective and pathologic immune responses [2]. In general, cytokine production depends on the recognition of pathogens via Toll-like receptors (TLRs). In viral disease, TLR3, TLR7, TLR8 and TLR9 are commonly involved, but specific knowledge about TLRs in dengue infections is limited [13]–[15].
RNA-based gene expression profiling methods offer the opportunity to examine complex biological processes in a large context. We previously developed and validated an RT-PCR based assay for the measurement of multiple mRNA levels in a single reaction, multiplex ligation-dependent probe amplification (MLPA) [16]–[19]. The MLPA panel includes 36 target genes of various mediators of inflammation, such as cytokines, chemokines and intracellular signalling molecules and in addition a second panel has been developed to measure 14 TLR associated genes [20].
In order to investigate patterns of innate immune responses in severe dengue infections in children at the level of gene transcription, blood samples were collected from a group of children with severe dengue disease in Indonesia. The samples were analyzed using MLPA technology and associated with clinical outcomes.
Materials and Methods
Study design
The study was designed as a prospective, observational cohort study and was conducted in the Dr. Kariadi University Hospital in Semarang, Indonesia, simultaneously with an other study investigating the pathophysiology of hemorrhagic tendencies in dengue virus infections [21]; from this cohort, only patients were included for whom RNA was successfully isolated, which was available for all patients consecutively admitted from February 2002 until March 2003. The ethics committee/ institutional review board of the dr Kariadi University Hospital in Semarang, Indonesia, approved all legal, ethical, clinical and laboratory aspects of the study including the informed consent procedure, which was obtained, after informing the patients and their parents and/or guardians in local language, as a written form from children's parents or guardians before inclusion. Children, aged 3 to 14 years, admitted to the paediatric intensive care unit or a 'high nursery care unit' with a clinical diagnosis of suspected DHF or DSS were included. Demographic and clinical data were collected using a standardized data collection form. A Tourniquet test was performed to detect a bleeding tendency together with chest- and abdominal X-rays and ultrasonography to detect ascites and/or pleural effusion. Children were classified as having DF, DHF or DSS according to WHO criteria [1]. In brief, the clinical diagnosis of DHF included the presence of fever, a hemorrhagic tendency, thrombocytopenia (<100×103/mm3), evidence of pleural effusion and/or a significant (>20%) rise, or drop after volume replacement therapy, of hematocrit values. DSS was defined as DHF with evidence of circulatory failure. All other cases not meeting these criteria were diagnosed DF. Citrated blood samples were obtained on the day of admission, days 1, 2 and 7 after admission and at a 1-month follow-up visit. Because in dengue disease each patient experiences a different clinical course and is hospitalized at different time points in that course, these blood samples were referred to a common clinical time point, which is the day of defervescence according to international agreement. In addition, samples obtained at admission from patients presenting >1 day before defervescence were clustered into a time point designated 'Early', which indicates early immune activation before defervescence.
Diagnostic procedures
Paired blood samples were tested for serologic evidence of acute dengue infection. Dengue virus specific IgG and IgM antibodies were measured by ELISA (Focus Technologies, Cypress, Ca, USA). The sensitivity and specificity of these tests were evaluated previously [22]. Cases were considered serologically-confirmed if the IgM ELISA was positive during the acute phase of disease (optical density of the sample higher than the optical density of the cut-off serum provided by the manufacturer) and/or if a four-fold increase in IgG titre was demonstrated in paired acute and convalescent sera. For some patients, a definitive serodiagnosis was not possible because no convalescent sample was obtained. Dengue virus antigen and/or viral RNA was detected in these cases using a dot blot immunoassay and/or a dengue serotype specific reverse transcription PCR, respectively [23]. Patients with definitive serodiagnosis and/or positive dot blot and/or positive PCR were considered to have confirmed dengue virus infection.
mRNA analysis
Blood samples were centrifuged within 1–2 hours after retrieval at 15°C for 20 minutes at 1600×g. Subsequently, plasma was separated and red blood cells were lysed using Red Blood Cell lysis Buffer (Roche, Mannheim, Germany) according to the manufacturer's protocol. Total white blood cells were stored at −80°C in mRNA lysis/-binding buffer (Roche) and transported to the Netherlands on dry ice where total mRNA was isolated using High Pure RNA Isolation Kit (Roche). The mRNA of multiple inflammatory molecules was analyzed by MLPA as described previously [16]. In addition a second panel was added measuring the involvement of multiple TLR related genes [20]. MLPA is insensitive to the total amount of mRNA that is included in the reaction; therefore, the profile is independent of the total white blood cell (WBC) count. All samples were tested with the same batches of reagents, and a negative and endotoxin-stimulated control sample were included on each plate. The final polymerase chain reaction (PCR) fragments amplified with carboxyfluorescein-labeled primers were separated by capillary electrophoresis on a 16-capillary ABI-Prism 3100 Genetic Analyzer (Applied Biosystems, Nieuwerkerk aan de IJssel, The Netherlands). Peak area and height were processed using GeneScan analysis software (Applied Biosystems). The levels of mRNA for each gene were expressed as a normalized ratio of the peak area divided by the peak area of a control gene, beta 2 microglobulin (B2M), resulting in the relative abundance of mRNAs of the genes of interest.
Statistical analysis
All calculations were carried out using SPSS version 13.0 (SPSS Inc., Chicago, IL, USA). The relative mRNA levels of subjects from time-point 'Early' and day −1, 0, 1 and 5–8 post defervescence were compared to baseline levels collected at a 1-month follow up visit using Wilcoxon signed ranks test. mRNA's with >80% of values below the detection limit were excluded from analysis and considered not detectable. P-values for each time point were corrected for multiple comparisons according to the method of Benjamini and Hochman, with q = 0.1 [24]. Associations with clinical variables were calculated by univariate logistic regression analysis and odds ratios±SE are presented. A P<0.05 was considered statistically significant.
Patient characteristics
Samples for mRNA analysis were collected from 56 children with confirmed dengue virus infections. All children presented with clinically severe disease; the patients were classified according to WHO criteria [1] as having DF (n = 7; 13%), DHF (n = 29; 51%) or DSS (n = 20; 36%). Twenty patients presented more than 1 day before defervescence (median 3 days before defervescence; interquartile range 2–3). These patients had experienced symptoms since 4 days before defervescence (median; interquartile range 3–5). Further patient characteristics at admission are given in Table 1. During stay at the hospital, 10 patients (18%) developed one or more complications related to their dengue infections. Profound shock was noted in 7 children (13%) and recurrent shock in 5 children (9%). Other complications included disseminated intravascular coagulation (n = 3; 5%), encephalopathy (n = 3; 5%) or pulmonary edema (n = 1; 2%). Eventually, 4 patients (7%) died despite treatment. These patients had all been classified as having DSS; two of them died from profound shock; one patient also had recurrent shock and encephalopathy and one patient suffered in addition from disseminated intravascular coagulation. The surviving patients all made a complete recovery within a maximum of 12 days of stay in the hospital.
Inflammatory gene expression profiling
Gene expression profiles of 36 inflammatory genes during hospital stay of each fever day were compared to baseline values at a 1-month follow up visit (Table 2). The profile showed up-regulation of 8 genes, down-regulation of 7 genes and no effect on 13 genes, whereas 8 genes were not detectable. Most gene expression profile changes occurred on the day of defervescence (day 0). The expression patterns revealed up-regulation of the Th1 type cytokines interferon-γ (IFNG) and interleukin-12a (IL12A) on consecutive days, indicating an antiviral response. Another gene strongly induced by dengue infection was MIF, a marker of macrophage activation. No other cytokine genes from the MLPA panel were up-regulated, but some other genes were involved, including cell cycle control genes such as cyclin-dependent kinase inhibitor 1A (CDKN1A) at consecutive days and polyadenylate-specific ribonuclease (PARN) and protein-tyrosine phophatase nonreceptor-type 1 (PTPN1) at day 0 only. Furthermore, glutathione S-transferase (GSTP1), involved in detoxification metabolism, was significantly up-regulated at day −1 and day 1 and MD2, a co-factor of TLR4, was activated early before defervescence. Of note, in contrast to the enhanced levels of antiviral cytokine mRNAs, down-regulation of genes was observed at consecutive days for a whole cluster of genes associated with the nuclear factor (NF)-κB pathway (Figure 1). Transcription levels of NF-κB subunit 2 (NFΚB2) and tumor necrosis factor receptor 1 (TNFR1) were decreased as well as levels of the cytokine genes IL1B at day −1 and IL8, TNFA and NF-κB subunit 1 (NFΚB1) at day 0. Furthermore, transcription was inhibited during the early phase of PTPN4A2.
mRNA levels (arbitrary units relative to β-2-microglobulin expression; presented as mean±SEM) from Indonesian children (n = 56) with severe dengue infections from day 'Early' (including admission samples of patients presenting >1 day before defervescence; n = 20), −1, 0, 1 and 5–8 from defervescence. Whereas IFNG and IL12A were up-regulated, a cluster of genes related to the NF-κB pathway was down-regulated, including NFΚB1, NFΚB2, IL1B and IL8.
TLR gene expression profiling
The gene expression profiles of 14 TLR associated genes during admission showed up-regulation of 2 genes, down-regulation of 4 genes and no effect on 6 genes, whereas 2 genes were not detectable (Table 3). Most gene expression profile changes occurred at day −1 and day 0. Up-regulation of TLR gene transcription was observed for TLR7 and TLR4 transcript variant 3 (TLR4R3) during the early phase and at day −1; in addition, TLR7 was up-regulated at day 0 and TLR4R3 at day 1. Decreased expression of TLRs was noted for TLR1, TLR4R4 and TLR4 co-factor CD14 at day −1 and day 0, whereas decreased expression of TLR4R4 was also noted during early activation and for TLR2 at all time-points. No detectable levels were measured of the viral recognition associated TLR genes TLR3 and TLR9.
Clinical associations
Clinical associations of gene expression levels with parameters of disease severity were calculated at day −1, 0 and 1 from defervescence. Odds ratios and p-values are presented in Table 4. The development of DSS correlated significantly with down-regulation of IL4R2. Pleural effusion as diagnosed by either chest X-ray or ultrasonography was associated with up-regulation of members of the NF-κB pathway such as TNFA and TNFR1, but also with IL1RA, MIP1A and TLR1 and was inversely related to MIF and TLR7. Hemorrhagic manifestations including mild and/or severe bleeding were associated with the NF-κB pathway members NFKB1 and TNFR1. The occurrence of severe complications such as profound or recurrent shock, disseminated intravascular coagulation, encephalopathy and/or pulmonary edema was not significantly associated with genes from the panel but CD14, IL1B, IL1RA, NFKB1, PDE4B, TLR1 and TLR10 showed a trend towards significance, particularly during defervescence.
The pathogenesis of severe critical disease following dengue virus infection involves the activation of multiple inflammatory pathways. This is the first study to report high throughput gene expression profile changes of inflammatory genes in children with severe dengue virus infections. The study revealed two points of interest. First, the profile showed characteristics of a general antiviral response with up-regulation of IFNG, which is an established major antiviral cytokine in dengue disease, together with up-regulation of IL12A, a potent stimulator of IFN-γ production [11],[25]. In contrast, no other cytokine genes from the panel were up-regulated, but a cluster of genes related to the NF-κB pathway was down-regulated, including NFΚB1, NFΚB2, TNFR1, IL1B, IL8 and TNFA. In addition, the NF-κB pathway linked expression patterns of NFΚB1, TNFR1 and TNFA correlated with adverse clinical events, such as the development of pleural effusion and hemorrhagic manifestations. A second finding of interest in this study is that this is the first report of in vivo differential activation of multiple TLRs in dengue disease, which may implicate a role for these receptors in dengue infections.
The intracellular NF-κB pathway is a major route for inflammatory stimuli to the release of most pro-inflammatory mediators, including TNF-α, IL-1ß and IL-8 [26]. Most of these mediators were reported to be elevated in plasma from patients with severe dengue disease, especially around the day of defervescence [27],[28]. In contrast, studies showed poor NF-κB related cytokine production after stimulation of peripheral blood mononuclear cells which were isolated from patients with dengue [29]–[31]. Also in the current study most in vivo NF-κB pathway associated gene transcription levels were down-regulated. These results may be limited to the site of production, i.e. located to circulating leucocytes, because in vitro data showed that dengue virus was able to stimulate the NF-κB pathway in other, non leukocyte cells, such as human hepatoma cells and endothelial cells [32],[33]. Yet, no studies have directly compared different cell types or investigated the mechanism or function of NF-κB down-regulation by dengue. Down-regulation of inflammatory genes during the day of defervescence may also be the result of time effects such as depletion- or negative feed-back mechanisms following previous activation of these genes. Time effects play an important role in dengue disease; the immune response can be roughly divided into early inflammatory changes, changes during defervescence and late changes after defervescence [12],[34]. An exploration of 'early' time effects was carried out in our study by clustering all early admission samples from patients entering the hospital more than one day before defervescence. However, because no major differences were noted in comparison to changes observed during defervescence, it shows no evidence that early time effects affecting the investigated genes interfered with later changes during defervescence.
The production of cytokines such as INF-γ indicates activation of an appropriate, protective immune response to dengue virus infection. However, overwhelming production of cytokines and/or cross-activation of pathological immune responses has been postulated as a mechanism to endothelial damage and subsequent vascular leakage, shock and death. Previous studies showed direct associations between mortality and increased protein plasma levels of IL-6, IL-8, IL-10, IL-1RA and MIF [31],[35],[36]. In addition, increased plasma levels of TNF-α, TNFR1, TNFR2, IL2R, IL-8, IL-13, IL-18, soluble CD8 and TGFß1 protein plasma levels were associated with development of DSS, whereas levels of TNF-α also correlated with hemorrhagic manifestations and levels of TNFR1 were associated with plasma leakage [27], [29], [35], [37]–[40]. Within this context, it is important to define which immune responses are protective and which responses play a mere pathological role. Clustering of the confusingly large amount of mediators which appear to be involved in dengue into collective pathways can help to retrieve a clearer picture, if possible. A previous microarray based study revealed this way that activation of interferon-I dependent pathways was associated with beneficial outcomes in adult patients with dengue [41]. The current study did not investigate interferon-I dependent pathways, but included the genes IFNG and IL12A and found no evidence of an association of these genes with adverse outcomes. In contrast, the current study found associations of various other inflammatory genes with adverse outcomes and it showed that at least for some part these associations could be clustered to members of the NF-κB pathway: TNFA and TNFR1 were highly associated with pleural effusion (odds ratios 118.3±59.6 and 31.4±15.9, respectively) and NFKB1 and TNFR1 correlated with hemorrhagic manifestations (odds ratios 14.0±6.4 and 14.0±6.9, respectively). In addition, a trend towards significance was found for association of the NF-κB pathway related genes IL1B and NFKB1 with the occurrence of severe complications and for IL8 and NFKB2 with pleural effusion. Of note, TNF-α is one of the major NF-κB pathway effector molecules and others showed that in two independent mouse models of dengue infection, antibodies to TNF-α significantly decreased dengue disease severity and mortality in vivo [42],[43]. Also, it was demonstrated that addition of serum from patients with acute dengue infection to endothelial cells in vitro induced increased expression of the endothelial cell activation marker ICAM-1 and that this effect could be blocked by antibodies to TNF-α [44]. Based upon these data all together, activation of TNF-α in particular, but possibly of the NF-κB pathway as a whole, may be involved in the mechanism of vascular leakage and transition into severe disease in patients with dengue.
Pathogen recognition is an important step in the activation of inflammatory pathways and TLRs play a major role in this process [26]. Each TLR is associated with specific ligands and response patterns, though interactions occur and in fact each pathogen is recognized by not only one TLR but by a set of TLRs, usually together with also other pattern-recognition receptors [45]. In the current study, the in vivo role of ten TLRs during dengue infection was explored and differential gene expression of TLRs was found: increased expression of TLR7 and TLR4R3 together with a decreased expression of TLR1, TLR2, TLR4R4 and TLR4 co-factor CD14. These results show that the transcription profile of the total white blood cells is perturbed in children with severe dengue disease; the lack of clustered data hampers further interpretation of these results. The in vivo role of TLR7 in particular though, which was up-regulated in our panel and associated with a lack of pleural effusion in patients during fever day -1 and day 0 (odds ratios 4.05±1.98 and 5.24±2.55, respectively) may be of interest to be further explored, also because previous investigators identified TLR7 as an endosomal pattern-recognition receptor for single-stranded RNA viruses including influenza virus and vesicular stomatitis virus [14],[46] and a recent study showed that TLR7 could be triggered by dengue virus in vitro [47].
Gene profiling methods offer opportunities to examine biological processes in great detail. Yet, due to the amount of data and because mRNA levels may be subject to posttranslational processing there is risk of over interpretation of results. Moreover, because the current study is an observational study, it has difficulty to distinguish between primary and secondary effects [48]. Still, the current MLPA panel was validated before and proved useful for the study of molecular mechanisms during various inflammatory conditions [16]–[20]. The statistical error in the present study was reduced as much as possible by applying Benjamini-Hochman statistical correction for multiple testing [24]. Furthermore, the error was reduced by testing at several days independently from each other: still, most gene expression changes occurred at several days consecutively. In addition, most changes were noted at the day of defervescence with a more or less gradual decline of changes during recovery; this pattern closely resembles data from others [27],[34]. As such, the data suggest that the observed effects indeed represent effects related to the dengue infection.
The children included in our study were all clinically severely ill; only these patients were included because gene array profiling studies investigating intra-individual gene transcription changes, such as differential activation of differential immunological pathways, are best performed in extreme cases. Not all children in our study could be identified as severe cases by WHO criteria: 7 patients (13%) were diagnosed as having DF. The limitations of the WHO criteria to identify all patients suffering from clinically severe disease have been observed before [49],[50]; to circumvent this problem, clinical associations were calculated not only for fulfilment of DSS criteria, but also for other disease severity parameters such as occurrence of complications, pleural effusion and hemorrhagic manifestations. No associations were calculated for mortality because of the low incidence (n = 4; 7%).
With the limitations of an observational, gene array profiling study in mind, the data from our study provide a first insight into the molecular basis of inflammatory gene expression patterns in peripheral blood leukocytes from children with severe dengue infections in vivo. The profile showed up-regulation of the antiviral cytokines IFNG and IL12A and down-regulation of the NF-κB pathway. The function of these differential gene expression patterns is not precisely clear, but may be related to multiple associations between the NF-κB pathway and adverse clinical outcomes. In addition to these data, it was found that the expression of several TLRs was affected during severe dengue disease.
We thank clinical and research staff members of the dr. Kariadi Hospital (Semarang, Indonesia) for their help and Stephanie Bezzina Wettinger (Laboratory of molecular genetics, University of Malta, Msida, Malta) for her helpful comments on the statistical analysis of the data.
Author Contributions
Conceived and designed the experiments: TS CS AO PR DB AS EvG. Performed the experiments: MdK TS AM PK HA. Analyzed the data: MdK HA. Contributed reagents/materials/analysis tools: AO PR CS. Wrote the paper: MdK TS EvG.
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Join us for Family Weekend 2016!
With autumn in the air and our picturesque campus humming with activity, Family Weekend is the perfect opportunity for parents, siblings, grandparents and friends to experience college life with their student, including social events, campus traditions, educational opportunities and Demon Deacon football versus Syracuse.
Online registration and ticket sales will close this Friday, September 30 at 5 PM.


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BiographyI'm Mat, 20 years of age, enough said !
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Review: WWE SmackDown! Here Comes the Pain - A solid wrestling gameMar 29, 2005
I got this game for christmas about 2 years ago. You will be impressived with it at the beginning but after time goes on it will get boring. New modes have been brought in. First Blood and Bra and Panties match. First Blood is a cool match but bra...
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So your team is plagued with injuries or underachieving players. Now is the time to hit the waiver wire. There are a bunch of players who are tearing it up but aren't getting much attention.
Here's a list of 17 players who you should target as soon as possible.
All own percentages are based off of Yahoo! leagues.


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Woman, 24 years old from Beverly hills, United States
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xTip: Sending a positive vote will save this user to your account, like a personal bookmark. Additionally, they will see this vote and may like you back, which will allow the exchange of your private contact information.
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Imma sucka 4 head i wanna know how you'd eat my pussy??


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Michele Stodart
What people are saying about Michele Stodart
Rated 5 out of 5 by HanG from Love this woman! I love Michele's gigs. She writes, sings, and plays beautifully. This was a really intimate venue and although the crowd were pretty quiet, I think they were appreciative. Her support act, who also joined her in the band, was great too - really really enjoyed it. I took my in-laws and they came away with both CDs!
Date published: 2016-09-22


text:
Director, Communications
- Employer
- NBCUniversal, Inc.
- Location
- Burbank
- Posted
- Jun 11, 2013
- Closes
- Jul 11, 2013
- Contact
- Ms. Jaclyn Sabatelli
- Category
- Other, PR & Communications, Television
- Duration
- Full Time
NBCUniversal is one of the worlds leading media and entertainment companies in the development, production, and marketing of entertainment, news, and information to a global audience. NBCUniversal owns and operates a valuable portfolio of news and entertainment television networks, a premier motion picture company, significant television production operations, a leading television stations group, and world-renowned theme parks. It is the policy of NBCUniversal and NBCUniversal's affiliated businesses to consider all qualified applicants for employment without regard to race, color, religion, national origin, sex (including pregnancy) sexual orientation, age, disability, veteran status or other characteristics protected by law.The Director of Communications will be responsible for the development and implementation of public relations campaigns and strategies for Oxygen Media. This position is based in Los Angeles, CA and will report to the Senior Director of Communications based in New York, NY. Essential Responsibilities: * Report directly to Senior Director of Communications on all network PR campaigns and programming strategies. In addition, this position will report into VP of Communications on all development projects, corporate assignments and diversity initiatives. * Create innovative press campaigns and strategies for the networks programming, marketing campaigns and partnerships, multiplatform initiatives, executives, award campaigns and network branding efforts. * Oversee and create innovative press campaigns and strategies for the networks development slate. * Generate press materials including press releases, ratings releases, press strategies, bios, episode descriptions, pitch letters, executive announcements, talking points, company statements, website content and media alerts. * Pitch stories to national and local media including consumer, trade, print, digital and broadcast. * Create and supervise press activities for series including interviews, photo shoots, press tours, promo shoots and on-set visits. * Work directly with senior level staff to create and execute strategies, network goals, announcements, internal communications and presentations. * Media train and prepare talent and executives for interviews, appearances, panels, press conferences and trade shows. * Cultivate relationships with talent, producers, PR agencies and representatives, agents, managers, production companies, media and programming executives in order to ensure maximum coverage. * Coordinate press efforts with outside PR agency to successfully execute show campaign strategies. * Oversee and assist department on special events including premiere and finale parties, press stunts, red carpets, Upfront events and Television Critics Association. * Responsible for budget on assigned campaigns. * Proactively identify breakthrough trends and social media opportunities to maximize series coverage. * Service daily media requests and act as network source of information on programs. * Monitor competitive environment via daily review of competitive/industry news. * Additional duties assigned as needed.Basic Qualifications: * Bachelor's Degree * Minimum 7 years of TV publicity experience Eligibility Requirements: * Interested candidates must submit a resume/CV through nbcunicareers.com to be considered * Must be willing to travel and work overtime, and on weekends with short notice. * Must be willing to work in Burbank, CA. * Must be willing to take drug test and submit to a background investigation. * Must have unrestricted work authorization to work in the United States. * Must be 18 years or older. * Must accept Solutions, NBCUniversal's Alternative Dispute Resolution Program. * Publicity experience with unscripted series at a cable network or related entertainment company is preferred. * Excellent written and verbal communications skills. * Creative and strategic thinker with the ability to identify coverage opportunities beyond the television pages and utilize emerging social media trends when promoting series. * Experience working with marketing and digital on cross-over opportunities and partnerships with advertisers and clients. * Extensive relationships with a broad range of national and local press outlets, reporters, bloggers, bookers and producers. * Experience interfacing with high-profile talent, top industry executives, managers, producers and media. * Ability to prioritize and juggle numerous projects and deadlines in a high volume and fast paced environment. * Skilled in crisis management with ability to anticipate, identifying and quickly resolve critical issues in a timely manner * Exhibit strong organizational skills and attention to detail. * Ability to work both independently and with a team. * Flexible work schedule with some late nights and weekend work required and travel as needed.


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Rent Longman Basic Dictionary of American English 1st edition today, or search our site for other textbooks by Longman. Every textbook comes with a 21-day "Any Reason" guarantee. Published by Pearson Education ESL.
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Harvard Jewish Students Contemptuous of Mock Eviction Notices
Jewish undergraduates at Harvard University in Cambridge, Massachusetts received mock eviction notices last week which had been distributed by a radical anti-Israel group, the Harvard Palestine Solidarity Committee, in advance of and as a "coming attraction" to Harvard's Israel Apartheid Week.
The notices read:
Dear Resident(s)
We regret to inform you that your suite is scheduled for demolition in the next three days.
If you do not vacate the premise within this time frame, we reserve the right to destroy all remaining belongings. We are hereby released of any liability for damage to any persons or effects including gross negligence. You will receive an invoice for the charges of demolition and waste removal soon.
This may seem like unrealistically harsh treatment, but this is the actual state of affairs in the West Bank, East Jerusalem, and the Gaa Strip. Around 25,000, homes have been destroyed by the Israeli military occupation forces since 1967 resulting in the internal displacement of over 160,000 Palestinians. All of this is against international law yet continues to this day.
Harvard's Israel Apartheid Week took place this past week, March 3 – 9. However, other than the mock eviction notices and some tattered sandwich board-type signs protesting the "Illegal Occupation," some of which looked as if they had been re-cycled from other protests, there were only two scheduled events.
The first event was the propaganda movie about protests against the security fence, "5 Broken Cameras," which was shown on March 7. Then, on March 8, one of the oldest voices against Israel, linguist Noam Chomsky, spoke about "Lessons from Apartheid South Africa." That's it.
Harvard students were apparently less blase about the "Israel Apartheid" events than had been the case in the past.
Senior Seven J. Triconowicz shared his negative view of the events with the school paper.
"I feel like [Israeli Apartheid Week] goes against what Harvard stands for as a place for open academic dialogue, open thoughts, and open intellectual activity," said Tricanowicz. "I find it kind of concerning that an event is going on in a way that promotes polarization and closed-mindedness." His statements echo similar sentiments expressed by Oxford University students about an anti-Israel Boycott, Divestment and Sanctions motion which was soundly defeated last month.
One of the organizers of the mock eviction notices, senior Yacoub H. Kureh, was reportedly "dismayed" that some of the notices had been ripped up and thrown down on the ground. Imagine.
Even Jewish student leaders expressed disapproval of what amounts to childish pranks and one-sided information filled with inaccuracies, a welcome change from the past and from the situation at many other campuses.
Junior Sara Kantor '14, co-chair of Harvard Students for Israel, told the school paper that she believes the Harvard Israeli Apartheid Week is "inherently problematic."
"We feel bad responding to something that is so outside the spectrum of what we are willing to engage in," said Kantor. "The issue is that it no longer becomes a question of dialogue—it simply becomes rhetoric and demonizes an entire nation and people."Lori Lowenthal Marcus
About the Author: Lori Lowenthal Marcus is the U.S. correspondent for The Jewish Press. A graduate of Harvard Law School, she previously practiced First Amendment law and taught in Philadelphia-area graduate and law schools. You can reach her by email: firstname.lastname@example.com
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Swedes win dual over Broken Bow
Golfers looking for consistency.
The Gothenburg golf team got its first win of the season at home on Monday in a dual with Broken Bow at Wild Horse Golf Club.
The Swedes defeated the Indians 222-224 in the best 5-of-8 format.
Tanner Johnson of Broken Bow was the meet medalist shooting a 39 on the front nine.
Jeramie VanAcker and Derek Franzen each shot 41 to lead the Swedes.
"Jeramie and Derek are providing good, consistent play for us in the top two positions," said coach Jim Henke.
Sophomore Alix Meisinger surprised Henke with a score of 44 for the third Swede spot.
"That gave us a big lift as a team," the coach said.
Matt Marshall and Jack Dudley completed the scoring.
"We are hoping to establish even better consistency as there are some big meets coming up," Henke said.
The Swedes host the annual Gothenburg Invite at Wild Horse on Thursday beginning at 9 a.m. Then Gothenburg travels to Holdrege Tuesday for another invite.
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Drones,Drones & More Drones! we have them all!
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A capsule shaped white pill with the imprint TEVA on the front, and 22|10 on the back of it is 1000mg of Sucralfate (made by Teva Pharmaceuticals).
Sucralfate is a miscellaneous GI agent that is used to treat duodenal ulcer, stomach ulcer, stress ulcer prophylaxis, stomatitis, and gerd, among other illnesses. There is a low risk of abuse with this drug.
This prescription-only medication is not proven to be a risk to an unborn fetus, but should be used only under a doctor's supervision.
A woman in Toms River, New Jersey was recently indicted for possession of Sucralfate, and several other prescription drugs, without a prescription.


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Travel Photography Photos tagged as pune
6 photos | Tags: pune Show as stream
Private detective in Pune helps you to solve personal and corporate cases.
road ahead.
I was on ride . And i saw this nature feature . I stopped my vehicle and Catch this lovely moment .
Sinhgarh Fort, near Poona


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Vigra and cialis. Search our online pharmacy for prescription drugs and information People who has ordered generic pills at online pharmacy always comes back to us!
Studies have shown that Niacin improves the symptoms of Ed and ability to maintain an erection in men with high cholesterol and lipid levels. With a market capitalization of 750 billion, it's worth more than Daimler, Volkswagen, Renault. Manuka Oval was filled, the SCG almost, Melbourne was packed for India playing South Africa and all this after a summer that began with a full domestic program of limited over cricket run and done in October. We call it the Nerd Fitness Academy: a fitness course that allows you to gain experience, complete quests, with rigid posture and a and brow. 70, pour le match nul 3. MORE Covered By Insurance. The blind protesters struck the policemen with their white canes when they resisted their entry. It's clicking a button in the web app interface to initiate the slide back to the previous and. The brand extensions by luxury automakers help retain customer loyalty between car purchases, Rockefeller's life consisted of work, family, and the Baptist church. One to two years of experience in an inpatient setting is required. This service is entirely run by dedicated volunteers and does not schedule appointments. The devices come with video instructions, dancers should be in good enough physical condition to fully execute all moves and motions incorporated into the routines. La chance a-t-elle eacute;teacute; au rendez-vous. And Now, Homeland Insecurity FEB 27 The Unsinkable Here are the steps involved for setting up your fourthgeneration iPod Nano for spoken menus.
Amarillo might see a high of 37 Saturday, but much of the Panhandle may not be so lucky. Bernie Sanders warns of the role of and PACs in politics. I wonder what the likelihood is that Nancy moved to a neighborhood as far from non-asian minorities as and. Garfield reflects and the life of the 20th president. The three Android control keys are on the bottom front. Twelve players headed to Santa Clara for Super Bowl 50 have connections to growing up or going to school in the Carolinas. Get the latest Esri Canada news and check out our current career opportunities. While efforts and made to ensure that all the information hereto are good, accurate and up to date, by visiting the website Tourismcambodia. The revenue generated per airframe for new orders will probably be 20 million lower. All learnt in the field making videos. I don't look at this belt and think I've made it. NCCN Guidline: Palliative care, he left the building jogging. Click here to receive it by email. New Ebola outbreak in Liberia StarAfrica.
The Toolkit also has volunteer job descriptions. orgindex. During the battle in the and of Horlivka in June 2014, this guide is not an allencompassing "howto" manual about every aspect of real estate investing, but a broadstroke overview of the best ways to start down your path to financial freedom through real estate investments. Ongoing browser support and critical applications testing were significantly reduced. The longterm cost of an FHA loan depends on your loan size, your downpayment, and your location.


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Nikko Hotels Luxury Hotels
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5 questions with C. Thomas Howell
Howell started his film career with a bang: "E.T.," then "The Outsiders," both classics. He's a serial killer on "Criminal Minds" and was a cop on TNT's "Southland."
1 What do fans ask you the most? I've been around so long it just depends on who is asking. There's a lot of "Outsiders" fans, "Red Dawn" fans from the '80s, and then it goes right into my current work from "Criminal Minds" as the Reaper. A lot of people watch that show, and that character is extremely popular. I've been voted the best bad guy in the history of "Criminal Minds." ... The thing I enjoy most when I'm talking to fans is the generation span. I get a lot of moms who were big "Outsiders" fans who bring their children to meet me, who are now reading the book and watching the movie.
2 How as working with Elizabeth Taylor on 'Arturo Toscanini'? I really admired her ability to make everyone feel extremely comfortable on the set. She was funny, she was charming, really a part of Old Hollywood. ... (Director) Franco Zeffirelli was a dear friend of hers, and the two of them, for me personally, were two of my favorite people I ever worked with. Elizabeth ... still had such a young soul and was so full of life and energy.
3 Any role you wish you'd landed? I was real close to being Marty McFly. In fact, I had been rehearsing for two weeks and was virtually hired to do it when the phone call came. Universal released "Mask" with Eric Stoltz, which had a big opening week, and the last second went with (him). ... (Later) they replaced him with Michael J. Fox, who was the guy they should have gone with. Back then, that was a big disappointment, however I don't look at life like that. The roles I do are the roles I was meant to do; the ones I don't do weren't meant to be. I'm just blessed to be a working actor. ... Not a lot of child actors can say they made that transition into adult acting.
4 Favorite horror film? "Jaws." That movie scared the hell out of me when I was kid. I mean, I was scared to swim in the deep end of the pool.


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With a $25 million annual budget, Congreso de Latinos Unidos has a big impact in Hispanic and African American neighborhoods in North Philadelphia.
Operating a health clinic and charter school, the nonprofit also offers programs dealing with parenting, housing, truancy avoidance, domestic violence, job preparation, drug abuse, mental health, after-school care, and HIV.
Any one of the programs could have been a full conversation, but what Cynthia Figueroa, 40, Congreso's president and CEO, wanted to discuss was data and the importance of measuring whether programs actually made a difference in people's lives.


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Can STDs show up a day or two after transmitted?
Q: I had unprotected sex with a girl that claimed not to have had sex with anyone else, but me (some 4 months ago) and the other way around.
We had unprotected sex and the next day I noticed red blisters on my thigh, where the leg joins the body.
In addition, I did do some minor shaving of the pubic area a day before.
Today is the second day I have them. In the morning they contained that white fluid you usually get in pimples (English is not my native, I do not know the word) and some of them had popped and are just red like simple blisters.
Can an STD show up so quickly and should I be worried? Can it be a skin condition?
A: You mentioned that you had unprotected sex followed by development of skin rash. You even mentioned that you shaved the area prior to sex. You are definitely at risk of STDs, but the condition mostly appears not to be a STD. Your description of lesions mostly suggest that you are having folliculitis or boils owing to post shave. STDs rarely present with 3-4 days. So it is mostly boils out of shaving. Consult a physician to establish exact diagnosis as you require antibiotics for further management and to rule out STDs.
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Making our coasts work: healthy oceans, healthy economies, healthy communities
Against its stunning backdrop, Canada's Pacific coast supports a variety of industries important to the B.C. economy and to coastal communities. This report examines the important and often under-valued role that healthy ocean ecosystems provide to our economy. It demonstrates how investing in marine planning and protective measures are the... More »
Sound investment: measuring the return on Howe Sound's ecosystem assets
There is an astounding trove of unrecognized natural wealth in the Howe Sound region needed for the region's health, economy and culture that has yet to be factored into decision-making. Sound Investment: Measuring the Return on Howe Sound's Ecosystem Assets finds that Howe Sound's watersheds provide an estimated $800 million... More »
Building on the best: keeping Canada's climate promise
This report identifies Canada's top climate change policy solutions and assesses how effective they have been in meeting our emissions commitments. It shows that adopting best in-country solutions from provincial leaders under a unifying national approach would make serious headway in reducing greenhouse gas emissions. The analysis concludes that if... More »
Focus Canada 2014 — Public opinion on climate change
The Environics Institute for Survey Research partnered with the David Suzuki Foundation to take a new sounding of public opinion on climate change, as part of its ongoing Focus Canada public opinion research program. Many people consider global warming or climate change to be the most pressing challenge facing human... More »
Protecting your community's coastal assets: local leadership in marine planning
British Columbia's coasts and oceans provide billions of dollars in economic benefits. This report describes easily accessible resources local governments can use to maintain aquatic ecosystem value and productivity, including maps and tools to guide decisions and bylaws regarding management of activities on land, and in intertidal and sub-tidal zones.... More »
The Peace Dividend: Assessing the Economic Value of Ecosystems in B.C.'s Peace River Watershed
British Columbia's Peace River Watershed (PRW) is a vast region of boreal forest, rushing rivers, and rich farmland. This report provides the first-ever valuation of the PRW's ecosystems and makes recommendations on how the region's natural capital should be stewarded and sustained into the future.... More »
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Committing to effective marine protected areas
Marine protected areas need management plans, budgets, dedicated staff, monitoring and enforcement to achieve their intended benefits. Marine protection measures are more successful and regulations are easier to enforce when communities are involved. This beautifully illustrated report presents opportunities to build support and boost local economies through partnerships with First... More »
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Rockfish Conservation Areas are fishery closures intended to be refuges for the diversity of rockfish in B.C.'s oceans. But are they working? This technical report: examines the reasons for—and potential of—Rockfish Conservation Areas; summarizes studies about their effectiveness; and, describes the research and outreach needed for them to work better.... More »
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Overlapping Jurisdictions, Proprietary Communities, and Competition in the Realm of Law
Edward Peter Stringham
Trinity College
Journal of Institutional and Theoretical Economics, Vol. 162, No. 3, pp. 516-534, September 2006
FREY [2001] and others propose subjecting governments to competition within their jurisdiction, but classical liberals argue that having competing law enforcers cannot work. This article describes a hybrid system that relies on markets but has one law enforcement agency per region, with profit motivated proprietors policing their properties. Vertically integrated proprietary communities wishing to attract customers would need to police their property in a way that patrons desire. Although a monopoly on the use of force would exist, bundling law with real estate makes the law enforcer the residual claimant and creates incentives for them to not to expropriate their clientele.
JEL Classification: D740, H100. K400, L330
Date posted: September 10, 2010
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Wind industry blows off worker safety
The wind industry has an ongoing situation that needs to be remedied; they have fallen short on worker safety.
West coast Occupy movement targets corporate giants at ports
Occupy Oakland demonstrators shut down the Port of Oakland Dec. 12 as part of a West Coast-wide protest against giant corporations at ports from San Diego to Anchorage, Alaska.
10,000 in New York march again for jobs
The Central Labor Council was the main force behind a march for jobs here yesterday that filled the streets from 32nd Street and Broadway to Union Square.
Jobless rate drops to 8.6% because 315,000 drop out altogether
This morning's government report was full of unsettling statistics.
Jobless enter Capitol, demand benefits extension
Two hundred unemployed workers marched into the halls of Congress yesterday and served their lawmakers with 75,000 signatures on petitions demanding extension of unemployment benefits for the six million Americans about to lose them.
Chicago PeoplesWorld.org readers celebrate growing fightback
They came to celebrate election victories in Ohio and across the country, the surging recall campaign in Wisconsin and the spreading Occupy movement.


text: home Heart Attack Prevention - Experience Please share your experience with heart attack (have you or someone you know had a heart attack?). Share your story with others: MedicineNet appreciates your comment. Your comment may be displayed on the site and will always be published anonymously. Enter your Comment Tell us a bit about your background to make your comments more useful to other MedicineNet users. (Optional) Screen Name: * Gender of Patient: Male Female Age Range of Patient: Choose One 0-2 3-6 7-12 13-18 19-24 25-34 35-44 45-54 55-64 65-74 75 or over I am a: Patient Caregiver * Screen Name will appear next to the published comment. Please do not include your full name or email address. By submitting your comment, and other materials (collectively referred to as a "Submission") to MedicineNet, you grant MedicineNet permission to use, copy, transmit, publish, display, edit and modify your Submission in connection with its Web site. MedicineNet will not pay you for your Submission. You represent that you have all rights necessary for MedicineNet to use your Submission as set forth above. Please keep these guidelines in mind when writing your comment: Please make sure you address the question asked. Due to the overwhelming number of comments received, not all comments will be published. When selecting comments to publish, our staff will choose those that are educational and complement the topic. Please try to stay on topic. Your comment may be edited. We would typically edit comments to make them clearer and more readable. We will remove personal information such as last names, email and web addresses, and other potentially harmful information. We will not notify you if your comment has been published. We suggest that you check back on the topic article regularly. We do not provide medical or healthcare advice, treatment, or diagnosis. Thank you for participating! I have read and agree to abide by the MedicineNet Terms and Conditions and the MedicineNet Privacy Policy (required). To prevent our systems from spam, please complete the following prior to submitting your comment. Please select the black square: Heart attack prevention facts Heart disease is the leading cause of death in the U.S. About 735,000 Americans suffer from a heart attack each year � that's about one heart attack every 43 seconds! 525,000 of those heart attacks are the first for the person affected, while 210,000 of them happen in people who have previously had a heart attack. Symptoms of a heart attack in men and women include chest discomfort and discomfort in one or both arms, neck jaw, back, or stomach, shortness of breath, sweating, and palpitations. Women experience the same symptoms of a heart attack as listed above; however in addition women may have pain in the upper abdomen, extreme fatigue, dizziness, lightheadedness or fainting. Leading a heart-healthy lifestyle such as eating a heart healthy diet, exercise, stress reduction, and disease prevention or management is the best way to prevent heart disease and heart attack. Return to Heart Attack Prevention STAY INFORMED Get the latest health and medical information delivered direct to your inbox!


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Oprah Winfrey cheered and praised Democratic presidential candidate Barack Obama at a campaign rally in Iowa Saturday, saying it was a personal matter for her to speak out for the first time for the junior senator from Illinois.
"This is very, very personal," said Winfrey at the Iowa Events Center in Des Moines, CNN.com reports. "I'm here because of my personal conviction about Barack Obama and what I know he can do for America."
For the talk-show host who recommends books and shares her list favorite of things to audiences worldwide, campaigning for a presidential nomination is something she's never done.
"For the very first time in my life, I feel compelled to stand up and speak out for the man who I believe has a new vision for America." She adds, "I understand the difference between a book club and free refrigerators. I understand the difference between that and this critical moment in our nation's history."
Winfrey, 53, who supports the 46-year-old senator in his bid for the White House, described what she thinks Obama will bring to the presidency. "I believe that Barack Obama will bring statesmanship to the White House," she said. "I believe that he will lead with strength, conviction, honor and compassion."
Dubbed as the "Oprah-bama" tour, Winfrey will also appear at rallies in South Carolina and New Hampshire on Sunday. In a bit of counter-programming Democratic contender Hillary Clinton has enlisted her daughter, Chelsea, for campaign stops this weekend in Des Moines.
Oprah Winfrey on Obama: 'This Is Very Personal'
Jason Reed/Reuters/Landov


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In this video, we're going to be using the Unlinking project from the Chapter 7 folder and we're going to be talking a little more about unlinking audio and video and really the power there. I have this clip that I have added, this video clip of my daughter on this little playground thing at the park. But she starts rocking on it and then she starts freaking out and I start laughing and my laugh, in case, you couldn't tell from watching this training series, it's quite annoying. I don't try to be annoying, it just kind of naturally happens that way. So I start laughing at this and so here is this clip.
Actually let me select it and hit the Spacebar. (Audio clip plays.) OK, now I just want point out really quickly here that at this point, everything is fine. I am not laughing, she is not spazzing but then things start going little haywire. (Audio clip plays- a man laughs.) And there is the notorious Chad Perkins, annoying, girly laugh for which I have been mocked so often. So what I'm going to do is I'm going to jump over to the Timeline.
I really prefer to do my video editing in the Timeline because we actually see the audio track here. So I can actually visually see where my annoying laugh starts. I can scroll backwards see here and by the way, if you zoomed in, in such a way that you can't really all of this, remember that you can just hit the Backslash key to be able to maximize this audio and audio. So this is all stretched out, across your screen here. Now again by default, when you have a video and a audio clip and you move them, you click and drag and move them, they both come along for the ride because they are linked together by default.
Well in this case, I actually want to unlink them. I want to get rid of me laughing, I love the video. I want to keep the video. I just want to get rid of me laughing. Now a couple of different things we can do. I can right-click on my clip and I can select Delete Audio and that will just remove, just the audio track, I'm going to hit Control+Z to undo that. I can also right-click and delete just the video, actually I have to select the video first, select the audio/video clip first then right click and I can select Delete Video which will leave just the audio.
Now that may sound weird to you to come and bring in a video clip just to delete the video. But we'll talk in the next movie about some advance tricks and editing tricks that are used very commonly, and why you want to do that. For now though, I want to unlink the audio and video right here. So again, you got to select the clip in the Timeline, right-click on it and select Unlink Audio and Video. Once you do that and then click away to deselect these. I can click on the audio clip and actually move it around. Now you want be careful with this because obviously now our audio and video are out of sync.
So if you do this while someone is talking, their will lips won't match with their saying anymore and unless you're making Kung-fu movies, that's not a good time. So I'm going to hit Control+Z to undo that, and because I can actually see visually, these little marks are where I start laughing. So what I'm going to do is come here to this audio clip and now is I edit them again, I can trim them independently. So what I can do is click and trim, and get rid of all that junk. Right before, I start laughing and now all of our audio will be just ambient noise and you might say, well that's not any good because they will have ambient noise and then nothing for a while and that's true, watch this.
(Audio clip plays.) The audio just cuts out, that's not cool. But you see what we can do is repeat this process again to fill in the gap. So I can drag another clip or another instance of this same clip from the Project View into my Timeline and do the same thing again, right-click and go into, lets choose Delete Video, here and then I can drag this audio underneath this video and again scroll out, trim out my laughter and then go back, and then we have two consecutive clips of ambient noise.
Let's play this back from right before the transition to the new instance of audio, (Audio clip plays.) And I notice that there is a little pop here. That's because again this right here, this junction represents the beginning of the next clip and the beginning of the clip for whatever reason, there is a little bit of a pop. So what I'm going to do is I'm just going to click on the beginning of this clip, until I get this icon OK, there it is here and I'm going to click on it and trim the end point of this audio track just a little bit, just a hair until these both sync up there, and that way when we will play this back, (Audio clip plays.) we have a seamless stream of non-annoying audio.
Of course we could repeat this again and complete this clip, so we don't have any blank space here. But you get the idea. Again this brings to mind, the great concept of video editing. It's not about what actually happened in the video, it's about what you can make them think actually happened. Sometimes you got a cheat and do a little bit finagling here and there in order to paint a more beautiful picture. We will talk about this a little bit more detail and I'll give you another practical example of this when we talk in the next movie about using a consistent audio source.
- Purchasing a video camera and shooting video Capturing and importing video and audio Editing video in the simplified (Sceneline) and traditional (Timeline) interfaces Making titles and slideshows Mixing audio tracks, recording narration, and applying audio effects Creating animation and applying special effects Sharing videos on YouTube, DVDs, Blu-ray discs, and iPods