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Home > Eyeglasses > Nike > NIKE 8097 (068)
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NIKE 8097/068 Eyewear
As a customer of Edel-Optics, you are totally aware that eyewear can crown your style. This accessory makes you look very smart when you are out and about and convincing in the office and in your leisure time. Would another colour not be a better match for your favourite outfit? Check also the other styles of NIKE 8097in our 2015, and 2016 Nike range.
The frame is specially designed for men. Sporting clear lines, New School Cool meets traditional quality. The semi-rim frame has an emphatic lightness which is perfectly suitable for different occasions and different outfits. The quadratic shape makes the face appear longer, due to its straight outer edges. The frame is, therefore, particularly advantageous for more rounded facial features. The metal frame is very light and comfortable to wear. It is both robust and classy.
Moreover, we can also provide you with the correct prescription lenses at an incredibly low price. The only thing you need to provide us with are your current dioptre values determined by your ophthalmologist. Together with our digital dispensing optician, you can choose between reasonably priced brand lenses from Germany or premium brands such as Seiko, Hoya and Rodenstock. Edel-Optics' plastic lenses have many features such as anti-reflection coating, lotus effect, cleaning coating, antistatic and hard coating at no extra charge.
The next shipment is on its way, so we will have your Nike back in stock very soon. We hope the unbelievably low price will be a consolation for the short wait. And because Edel-Optics is a paradise for bargain hunters, you can get this top model at an incredibly low price. What is labelled as "in the sale" at other online shops, is for us "all-day-everyday" prices.
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Smart and sustainable learning for professionals. With impact. Learning solutions that actually work. Products that create a difference. Not just for now or a couple of months. But as long as needed. That's what we deliver.
Easily said, but we actually do it. For example: If emailing a pdf document to all employees delivers the same result as an e-learning module, we urge our client to use the pdf document. It's the best thing to do for the client. It's smart.
New professionals long for information about their job, the company and the people they work with. Eager to hop on the riding bus they come to the office in their new shoes and haircut; ready for a new journey.
Whether your job is operating heavy offshore machinery, transporting vegetables, working in a bank or a call center; your knowledge and skills make you a unique professional. We help you to learn the right skills for your job and to learn the rules.
Climbing the ladder or diving into the subject matter; your career can go in various directions. Regardless of the direction, you should hold the steering wheel firmly in your own hands.
We love projects with a bite, clients with guts, and being adventurous. The fine line between smart and cheeky is often crossed - on purpose. When necessary, we take a clever short-cut when everyone else follows the main road. Or we stay on the beaten track where others wander off.
It's a busy summer and we are still working on our new website. If you would like to know more, please let us know. We're happy to tell you all about our work.
The way we generate and consume gas and especially electricity is rapidly changing. The grid companies are the key stakeholder in this process; their employees need to be trained and informed on these new developments. This is where 'Altijd aan' enters the stage. 'Altijd aan' literally means 'always on'. In other words: always ready, always stand-by, always up-to-date.
Ampelmann's Framework for Growth is a development plan for operators: from new hire to senior. An online platform (operatortraining.ampelmann.nl) bundles all e-learning, simulation, practical training and learning on the job. With this framework Ampelmann continues to deliver a safe transfer between ship and offshore location.
How do you recognize a phishing email? Which attachments are safe to open? And which are absolutely not safe? Over 50,000 ING employees worldwide should be able to answer these questions. Or at least know where to find the right answer.
A vessel wants to enter the Rotterdam or Amsterdam harbor. How do you inform to the Harbormaster and Customs about this? By using Portbase' new Vessel Notification 2.0. It facilitates over one thousand ship agents with their service. An online application training helped all agents to start working with the new service quickly and easily.
In just two months' time we've created an e-learning module with the perfect flow. RMMBR created a customized solution, that extended beyond our original question.– Elise van Vliet (Portbase)
Everybody was enthusiastic about RMMBR's concept: an innovative design with short and powerful content based on real-life situations.- Madelon Trigt-Verdegaal (ING)
It looks amazing. It is nice to know that the module remembers where I was while I started the e-learning in the train, then switched off my laptop, and later opened the e-learning at the office.- Content Expert Alliander (Altijd Aan)
My compliments. It looks beautiful and it is user friendly.- Content Expert Stedin (Altijd Aan)

subset: commoncrawl


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Budd Terrace at Wesley Woods Admission
Upon admission, the patient/resident or family member/other responsible person should come to the Admissions Office, located on the first floor of the facility, where an Admissions Counselor will assist with the sign-in process.
Patients and residents should bring:
- Insurance Verification/Identification Cards (including Medicare and any supplemental insurance coverage).
- Living Will and Durable Power of Attorney for Health Care: a Living Will identifies patient wishes regarding end-of-life care in the event of debilitating health situations. A Durable Power of Attorney for Health Care is a document which gives another responsible individual the right to make healthcare decisions on behalf of the patient/resident if he/she is not able to do so. This document is not required, but Budd Terrace patients/residents are strongly encouraged to consider these issues.
- A Check for the First Billing Statement: if a patient/resident or family member will be paying for facility charges. This initial billing statement will be prorated at the daily private-pay rate for the number of days remaining in the current month. If the patient/resident is admitted after the 25th day of the current month, the initial billing statement will include the remaining days in the current month, plus the charges for the next month.
- Power of Attorney: this legal document gives another responsible individual the right to conduct the patient's/resident/s personal business affairs in the event the patient/resident is unable to do so. This is not required, but Budd Terrace patients/residents are strongly encouraged to consider these issues.
- Information Needed by Medical and Nursing Staff: if a patient/resident is admitting to Budd Terrace directly from home, assisted living facility, or personal care home, he/she may bring a pharmacy printout of all current medications or simply bring all current medication containers. The healthcare team must be notified of every medication (including over-the-counter medications) and supplements a patient/resident is taking.


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Making sense of Java on Linux
What To Do?
The good news is that there is now an easy option for free Java on Linux: Use OpenJDK with HotSpot. This option is free as in speech and free as in beer, and it is certified compatible with Sun's usual offering. (For most purposes, it is Sun's usual offering. If you have a recent major distribution, chances are you're already running it.)
On the other hand, if you want to experiment with different Java environments, that shouldn't be too hard either. Stable packages are available for several of the JVMs described in this article.
If you want to experiment with a lesser-known JVM, particularly if it is one of the versions dating to the early 2000s or late 1990s and it was not described in this article, you probably need to roll up your sleeves. Most likely, you won't have OpenJDK available, and you might not have GNU Classpath. If the project doesn't support Classpath or OpenJDK, you might have to settle for a less than complete set of libraries. The good news is, you don't have to play around with these partial solutions unless you really want to – if you do, you probably won't mind wrangling a few Makefiles.
- IcedTea: http://iced-tea.org/
- GNU Classpath: http://www.gnu.org/software/classpath/
- HotSpot JVM: http://openjdk.java.net/groups/hotspot/
- OpenJDK: http://openjdk.java.net/
- JamVM: http://jamvm.sourceforge.net/
- Cacao: http://www.cacaovm.org/
- Jikes RVM: http://jikesrvm.org/
- Kaffe: http://www.kaffe.org/
- SableVM: http://www.sablevm.org/
- GNU Compiler for Java: http://gcc.gnu.org/java/
- JVM list: http://www.kaffe.org/links
- IKVM: http://www.ikvm.net/
- Mauve: http://sources.redhat.com/mauve/
- Apache Harmony: http://harmony.apache.org/
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Nextcloud Announces Raspberry Pi-Powered Home Cloud Box
Innovative system adds a hard drive and Ubuntu Core to the RPi for an IoT hub.
Linus Torvalds Confirms the Date of the First Linux Release
Linux is two weeks younger than we thought!
End of OpenOffice?
The Apache Software Foundation considers retiring OpenOffice
Adobe Gives New Life to NPAPI Plugin for Linux
Adobe won't kill the plugin in 2017
LinuxCon North America Convenes in Toronto, Canada
Linux Foundation's big event celebrates the 25th anniversary of Linux
Linux Turns 25
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Competitors get in the game with RHEL without Red Hat
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Security researchers have already notified Microsoft; some fixes are available
Mirantis to Refactor OpenStack Fuel for Kubernetes
The company is collaborating with Google and Intel to use Kubernetes as an engine for Fuel
Canonical Joins Document Foundation Advisory Board
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Dress up your luau wardrobe with a Navy Blue Silk Lei!
The Navy Blue Silk Lei has navy blue flowers on each strand. The Navy Blue Silk Lei is 42 inches long and made of poly silk.
Please allow ample time for delivery. Our ship date for this product is noted in your shopping cart. Many items are shipped separately, so please allow extra time for these items to arrive. Shipping charges are based on the value of the merchandise and not the number of shipments. For additional shipping information, contact our Customer Service Department at (firstname.lastname@example.com or 1-800-227-2789).


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Most Cited Journal of Virological Methods Articles
The most cited articles published since 2011, extracted from Scopus.
Generation of a doxycycline-inducible KSHV producer cell line of endothelial origin: Maintenance of tight latency with efficient reactivation upon inductionJinjong Myoung | Don Ganem
Volume 174, Issues 1-2, June 2011, Pages 12-21
Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiological agent of Kaposi's sarcoma (KS) and at least two B cell lymphoproliferative diseases: primary effusion lymphoma (PEL) and multicentric Castleman's disease (MCD). B cells derived from PEL are latently infected, and can be induced to lytic replication by treatment with chemical agents like TPA or butyrate, which have pleiotropic effects on host cell signaling and chromatin structure. Most of these lines also display moderate levels of spontaneous lytic induction, which complicates analysis of latency. Here we describe the creation of latently infected cell lines derived from SLK endothelial cells that (i) display tight control of KSHV latency, with little spontaneous reactivation and (ii) are efficiently inducible by doxycycline, avoiding the need for pleiotropic inducing agents. These cells produce substantial quantities of infectious KSHV, and should be useful for studies of the latent-lytic switch and the impact of lytic replication on host cell biology. © 2011 Elsevier B.V.
Low-level detection and quantitation of cellular HIV-1 DNA and 2-LTR circles using droplet digital PCRTimothy J. Henrich | Sebastien Gallien | Jonathan Z. Li | Florencia Pereyra | Daniel R. Kuritzkes
Volume 186, Issues 1-2, November 2012, Pages 68-72
Droplet digital PCR (ddPCR) is an emerging nucleic acid detection method that provides absolute quantitations of target sequences without relying on the use of standard curves. The ability of ddPCR to detect and quantitate total HIV-1 DNA and 2-LTR circles from a panel of patients on and off antiviral therapy was evaluated compared to established real-time (RT)-PCR methods. To calculate the dynamic range of ddPCR for HIV-1 DNA and 2-LTR circles, serial dilutions of DNA amplicons or episomes were determined by ddPCR as well as with RT-PCR. HIV-1 DNA from 3 viremic patients and 4 patients on suppressive antiretroviral therapy, and 2-LTR circles from 3 patients with low-level viremia were also quantitated. Copy numbers determined by ddPCR of serial dilutions of HIV-1 or human CCR5 DNA amplicon standards were comparable to nominal input copy number. The sensitivity of ddPCR to detect HIV-1 or CCR5 DNA was similar to that of RT-PCR. Low levels of 2-LTR circles were detected in samples from all 3 patients by both ddPCR and RT-PCR. ddPCR is a promising novel technology for the study of HIV-1 reservoirs and persistence, but further optimization of this novel technology would enhance the detection of very low-level viral genetic targets. © 2012 Elsevier B.V.
Strand-specific real-time RT-PCR for distinguishing influenza vRNA, cRNA, and mRNAEiryo Kawakami | Tokiko Watanabe | Ken Fujii | Hideo Goto | Shinji Watanabe | Takeshi Noda | Yoshihiro Kawaoka
Volume 173, Issue 1, April 2011, Pages 1-6
Real-time RT-PCR is used to quantify individual influenza viral RNAs. However, conventional real-time RT-PCR, using strand-specific primers, has been shown to produce not only the anticipated strand-specific products, but also substantial amounts of non-strand-specific products, indicating lack of specificity. Therefore, in this study, a novel strand-specific real-time RT-PCR method was established to quantify the three types of influenza viral RNA (vRNA, cRNA, and mRNA) separately. This method is based on reverse transcription using tagged primers to add a 'tag' sequence at the 5′ end and the hot-start method. Real-time PCR using the 'tag' portion as the forward primer and a segment-specific reverse primer ensured the specificity for quantifying the three types of RNA. Using this method, specific target RNA was detected at 100-100,000-folds higher level than other types of RNA. This method was also used to evaluate the vRNA, cRNA, and mRNA levels of segments 5 and 6 in MDCK cells infected with influenza A virus at different time point post-infections. The cRNA level was 1/10 to 1/100 lower than that of the vRNA and mRNA. Moreover, different dynamics of vRNA, cRNA, and mRNA synthesis were observed; the copy number of the vRNA gradually increased throughout the infection, the cRNA increased and then plateaued, while the mRNA increased and then decreased. This novel method thus provides data critical for understanding the influenza virus life cycle, including transcription, replication, and genome incorporation into virions. © 2010 Elsevier B.V.
Digital PCR provides absolute quantitation of viral load for an occult RNA virusRichard Allen White | Stephen R. Quake | Kenneth Curr
Volume 179, Issue 1, January 2012, Pages 45-50
Using a multiplexed LNA-based Taqman assay, RT-digital PCR (RT-dPCR) was performed in a prefabricated microfluidic device that monitored absolute viral load in native and immortalized cell lines, overall precision of detection, and the absolute detection limit of an occult RNA virus GB Virus Type C (GBV-C). RT-dPCR had on average a 10% lower overall coefficient of variation (CV, a measurement of precision) for viral load testing than RT-qPCR and had a higher overall detection limit, able to quantify as low as three 5'-UTR molecules of GBV-C genome. Two commercial high-yield in vitro transcription kits (T7 Ribomax Express by Promega and Ampliscribe T7 Flash by Epicentre) were compared to amplify GBV-C RNA genome with T7-mediated amplification. The Ampliscribe T7 Flash outperformed the T7 Ribomax Express in yield of full-length GBV-C RNA genome. THP-1 cells (a model of monocytic derived cells) were transfected with GBV-C, yielding infectious virions that replicated over a 120. h time course and could be infected directly. This study provides the first evidence of GBV-C replication in monocytic derived clonal cells. Thus far, it is the only study using a microfluidic device that measures directly viral load of mammalian RNA virus in a digital format without need for a standard curve. © 2011 Elsevier B.V.
Inhibitory effects of silver nanoparticles on H1N1 influenza A virus in vitroDong xi Xiang | Qian Chen | Lin Pang | Cong long Zheng
Volume 178, Issues 1-2, December 2011, Pages 137-142
Silver nanoparticles have demonstrated efficient inhibitory activities against human immunodeficiency virus (HIV) and hepatitis B virus (HBV). However, the effects of silver nanoparticles against H1N1 influenza A virus remain unexplored. In this study, the interaction of silver nanoparticles with H1N1 influenza A virus was investigated. Silver nanoparticles with mean particle diameters of 10. nm were prepared for the hemagglutination inhibition test, the embryo inoculation assay, and the Mosmann-based 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, where these tests were used to determine the inhibitory activity of silver nanoparticles on H1N1 influenza A virus. MDCK cells were used as the infection model. Electron microscopy analysis and flow cytometry assay were used to determine whether silver nanoparticles could reduce H1N1 influenza A virus-induced apoptosis in MDCK cells. This study demonstrates that silver nanoparticles have anti-H1N1 influenza A virus activities. The inhibitory effects of silver nanoparticles on influenza A virus may be a novel clinical strategy for the prevention of influenza virus infection during the early dissemination stage of the virus. © 2011 Elsevier B.V.
Evaluation of a new, rapid test for detecting HCV infection, suitable for use with blood or oral fluidStephen R. Lee | Keith W. Kardos | Eugene Schiff | Cheryl A. Berne | Karam Mounzer | Alpha T. Banks | Harvey A. Tatum | Timothy J. Friel | Michael P. DeMicco | William M. Lee | Scott E. Eder | Alexander Monto | Graham D. Yearwood | Geraldine B. Guillon | Lisa A. Kurtz | Mark Fischl | Jay Lynn Unangst | Laura Kriebel | Gary Feiss | Michele Roehler
Volume 172, Issues 1-2, March 2011, Pages 27-31
The availability of a highly accurate, rapid, point-of-care test for hepatitis C virus (HCV) may be useful in addressing the problem of under-diagnosis of HCV, by increasing opportunities for testing outside of traditional clinical settings. A new HCV rapid test device (OraQuick® HCV Rapid Antibody Test), approved recently in Europe for use with venous blood, fingerstick blood, serum, plasma, or oral fluid was evaluated in a multi-center study and performance compared to established laboratory-based tests for detection of HCV.The HCV rapid test was evaluated in prospective testing of subjects with signs and/or symptoms of hepatitis, or who were at risk for hepatitis C using all 5 specimen types. Performance was assessed relative to HCV serostatus established by laboratory methods (EIA, RIBA and PCR) approved in Europe for diagnosis of hepatitis C infection. Sensitivity to antibody in early infection was also compared to EIA in 27 seroconversion panels. In addition, the reliability of the oral fluid sample for accurate detection of anti-HCV was assessed by studying the impact of various potentially interfering conditions of oral health, use of oral care products and consumption of food and drink.In this large study of at-risk and symptomatic persons, the overall specificities of the OraQuick® HCV Rapid Antibody Test were equivalent (99.6-99.9%) for all 5 specimen types and the 95% CIs substantially overlapped. Overall sensitivities were virtually identical for venous blood, fingerstick blood, serum and plasma (99.7-99.9%). Observed sensitivity was slightly lower for oral fluid at 98.1% though the upper CI (99.0%) was equal to the lower CI for venous blood and fingerstick blood. Most of the HCV positive subjects which gave nonreactive results in oral fluid had serological and virological results consistent with resolved infection. Sensitivity for anti-HCV in early seroconversion was virtually identical between the HCV rapid test and EIA. Detection of anti-HCV in oral fluid appeared generally robust to conditions of oral health, consumption of food and drink and use of oral care products.The OraQuick® HCV Rapid Antibody Test demonstrated clinical performance that was equivalent to current laboratory-based EIA. This new, HCV rapid test appears suitable as an aid in the diagnosis of HCV infection and may increase testing opportunities due to its simplicity and flexibility to use multiple specimen types, including fingerstick blood and oral fluid. © 2010 Elsevier B.V.
Validation of an internally controlled one-step real-time multiplex RT-PCR assay for the detection and quantitation of dengue virus RNA in plasmaKien Duong Thi Hue | Trung Vu Tuan | Hanh Tien Nguyen Thi | Chau Tran Nguyen Bich | Huy Huynh Le Anh | Bridget A. Wills | Cameron P. Simmons
Volume 177, Issue 2, November 2011, Pages 168-173
Dengue is mosquito-borne virus infection that annually causes ~50 million clinically apparent cases worldwide. An internally controlled one-step real-time multiplex RT-PCR assay was developed for detection and quantitation of DENV RNA in plasma sample by using specific primers and fluorogenic TaqMan probes. All primers and probes targeted sequences near the 3' end of the NS5 gene. The method comprised two multiplex assays and was validated for sensitivity, specificity, linearity, reproducibility and precision. An internal control template was spiked into each clinical specimen to provide quality assurance for each experimental step. The assay allowed for detection of between 0.5 and 3 infectious particles per mL, is rapid and has been operationally characterized in 287 Vietnamese dengue patients from two therapeutic intervention trials at the Hospital for Tropical Diseases, Ho Chi Minh City, Viet Nam. © 2011 Elsevier B.V.
Evaluation of rapid and simple techniques for the enrichment of viruses prior to metagenomic virus discoveryRichard J. Hall | Jing Wang | Angela K. Todd | Ange B. Bissielo | Seiha Yen | Hugo Strydom | Nicole E. Moore | Xiaoyun Ren | Q. Sue Huang | Philip E. Carter | Matthew Peacey
Volume 195, Issue , January 2014, Pages 194-204
The discovery of new or divergent viruses using metagenomics and high-throughput sequencing has become more commonplace. The preparation of a sample is known to have an effect on the representation of virus sequences within the metagenomic dataset yet comparatively little attention has been given to this. Physical enrichment techniques are often applied to samples to increase the number of viral sequences and therefore enhance the probability of detection. With the exception of virus ecology studies, there is a paucity of information available to researchers on the type of sample preparation required for a viral metagenomic study that seeks to identify an aetiological virus in an animal or human diagnostic sample. A review of published virus discovery studies revealed the most commonly used enrichment methods, that were usually quick and simple to implement, namely low-speed centrifugation, filtration, nuclease-treatment (or combinations of these) which have been routinely used but often without justification. These were applied to a simple and well-characterised artificial sample composed of bacterial and human cells, as well as DNA (adenovirus) and RNA viruses (influenza A and human enterovirus), being either non-enveloped capsid or enveloped viruses. The effect of the enrichment method was assessed by both quantitative real-time PCR and metagenomic analysis that incorporated an amplification step. Reductions in the absolute quantities of bacteria and human cells were observed for each method as determined by qPCR, but the relative abundance of viral sequences in the metagenomic dataset remained largely unchanged. A 3-step method of centrifugation, filtration and nuclease-treatment showed the greatest increase in the proportion of viral sequences. This study provides a starting point for the selection of a purification method in future virus discovery studies, and highlights the need for more data to validate the effect of enrichment methods on different sample types, amplification, bioinformatics approaches and sequencing platforms. This study also highlights the potential risks that may attend selection of a virus enrichment method without any consideration for the sample type being investigated. © 2013 The Authors.
One-step real-time reverse transcription-PCR assays for detecting and subtyping pandemic influenza A/H1N1 2009, seasonal influenza A/H1N1, and seasonal influenza A/H3N2 virusesMina Nakauchi | Yoshihiro Yasui | Tatsuya Miyoshi | Hiroko Minagawa | Tomoyuki Tanaka | Masato Tashiro | Tsutomu Kageyama
Volume 171, Issue 1, January 2011, Pages 156-162
Pandemic influenza A/H1N1 2009 (A/H1N1pdm) virus has caused significant outbreaks worldwide. A previous one-step real-time reverse transcription-PCR (rRT-PCR) assay for detecting A/H1N1pdm virus (H1pdm rRT-PCR assay) was improved since the former probe had a low melting temperature and low tolerance to viral mutation. To help with the screening of the A/H1N1pdm virus, rRT-PCR assays were also developed for detecting human seasonal A/H1N1 (H1 rRT-PCR assay) and A/H3N2 influenza viruses (H3 rRT-PCR assay). H1pdm, H1, and H3 rRT-PCR assays were evaluated using in vitro-transcribed control RNA, isolated viruses, and other respiratory pathogenic viruses, and were shown to have high sensitivity, good linearity (R2=0.99), and high specificity. In addition, the improved H1pdm rRT-PCR assay could detect two viral strains of A/H1N1pdm, namely, A/Aichi/472/2009 (H1N1)pdm and A/Sakai/89/2009 (H1N1)pdm, which have mutation(s) in the probe-binding region of the hemagglutinin gene, without loss of sensitivity. Using the three rRT-PCR assays developed, 90 clinical specimens collected between May and October 2009 were then tested. Of these, 26, 20, and 2 samples were identified as positive for A/H1pdm, A/H3, and A/H1, respectively, while 42 samples were negative for influenza A viruses. The present results suggest that these highly sensitive and specific H1pdm, H1, and H3 rRT-PCR assays are useful not only for diagnosing influenza viruses, but also for the surveillance of influenza viruses. © 2010 Elsevier B.V.
Selection and characterization of DNA aptamers for use in detection of avian influenza virus H5N1Ronghui Wang | Jingjing Zhao | Tieshan Jiang | Young M. Kwon | Huaguang Lu | Peirong Jiao | Ming Liao | Yanbin Li
Volume 189, Issue 2, May 2013, Pages 362-369
Aptamers are artificial oligonucleotides (DNA or RNA) that can bind to a broad range of targets. In diagnostic and detection assays, aptamers represent an alternative to antibodies as recognition agents. The objective of this study was to select and characterize DNA aptamers that can specifically bind to avian influenza virus (AIV) H5N1 based on Systematic Evolution of Ligands by EXponential enrichment (SELEX) and surface plasmon resonance (SPR). The selection was started with an ssDNA (single-stranded DNA) library of 1014 molecules randomized at central 74nt. For the first four selection cycles, purified hemagglutinin (HA) from AIV H5N1 was used as the target protein, and starting from the fifth cycle, entire H5N1 virus was applied in order to improve the specificity. After 13 rounds of selection, DNA aptamers that bind to the H5N1 were isolated and three aptamer sequences were characterized further by sequencing and affinity binding. Dot blot analysis was employed for monitoring the SELEX process and conducting the preliminary tests on the affinity and specificity of aptamers. With the increasing number of selection cycles, a steady increase in the color density was observed, indicating that the aptamers with good binding affinity to the target were enriched. The best aptamer candidate had a dissociation constant (KD) of 4.65nM as determined by SPR, showing a strong binding between the HA and the selected aptamer. The specificity was determined by testing non-target AIV H5N2, H5N3, H5N9, H9N2 and H7N2. Negligible cross-reactivity confirmed the high specificity of selected aptamers. The developed aptamer was then applied for detection of AIV H5N1 in spiked poultry swab samples. The obtained aptamers could open up possibilities for the development of aptamer-based medical diagnostics and detection assays for AIV H5N1. (The H5N1 used in this study was inactivated virus.). © 2013 Elsevier B.V.
A single-tube multiplex PCR for rapid detection in feces of 10 viruses causing diarrheaPattara Khamrin | Makiko Okame | Aksara Thongprachum | Nattika Nantachit | Shuichi Nishimura | Shoko Okitsu | Niwat Maneekarn | Hiroshi Ushijima
Volume 173, Issue 2, May 2011, Pages 390-393
A novel multiplex polymerase chain reaction assay was developed to identify 10 viruses in a single tube. The assay was targeted to detect group A and C rotaviruses, adenovirus, norovirus GI, norovirus GII, sapovirus, astrovirus, Aichi virus, parechovirus, and enterovirus. A total of 235 stool samples were collected from infants and children with acute gastroenteritis in Kyoto, Japan, from 2008 to 2009, then tested by this novel multiplex PCR and compared with a multiplex PCR described previously, which used 3 primer sets. The novel multiplex PCR could detect the targeted viruses in 111 of the 235 (47.2%) stool samples. Of these, 9 out of 10 types of viruses were identified, including group A rotavirus, norovirus GII, enterovirus, sapovirus, adenovirus, parechovirus, group C rotavirus, astrovirus, and norovirus GI. In contrast, the multiplex PCR that used 3 sets of primers could detect the targeted viruses in 109 of the 235 (46.4%) stool samples. Among these, 8 types of viruses were identified, including group A rotavirus, norovirus GII, enterovirus, adenovirus, parechovirus, group C rotavirus, sapovirus, and astrovirus. The results suggested that the new multiplex PCR is useful as a rapid and cost effective diagnostic tool for the detection of major pathogenic viruses causing diarrhea. © 2011 Elsevier B.V.
Comparison of commercial systems for extraction of nucleic acids from DNA/RNA respiratory pathogensGenyan Yang | Dean E. Erdman | Maja Kodani | John Kools | Michael D. Bowen | Barry S. Fields
Volume 171, Issue 1, January 2011, Pages 195-199
This study compared six automated nucleic acid extraction systems and one manual kit for their ability to recover nucleic acids from human nasal wash specimens spiked with five respiratory pathogens, representing Gram-positive bacteria (Streptococcus pyogenes), Gram-negative bacteria (Legionella pneumophila), DNA viruses (adenovirus), segmented RNA viruses (human influenza virus A), and non-segmented RNA viruses (respiratory syncytial virus). The robots and kit evaluated represent major commercially available methods that are capable of simultaneous extraction of DNA and RNA from respiratory specimens, and included platforms based on magnetic-bead technology (KingFisher mL, Biorobot EZ1, easyMAG, KingFisher Flex, and MagNA Pure Compact) or glass fiber filter technology (Biorobot MDX and the manual kit Allprep). All methods yielded extracts free of cross-contamination and RT-PCR inhibition. All automated systems recovered L. pneumophila and adenovirus DNA equivalently. However, the MagNA Pure protocol demonstrated more than 4-fold higher DNA recovery from the S. pyogenes than other methods. The KingFisher mL and easyMAG protocols provided 1- to 3-log wider linearity and extracted 3- to 4-fold more RNA from the human influenza virus and respiratory syncytial virus. These findings suggest that systems differed in nucleic acid recovery, reproducibility, and linearity in a pathogen specific manner. © 2010.
Detection and quantitation of infectious human adenoviruses and JC polyomaviruses in water by immunofluorescence assayByron Calgua | Celia Regina Monte Barardi | Silvia Bofill-Mas | Jesus Rodriguez-Manzano | Rosina Girones
Volume 171, Issue 1, January 2011, Pages 1-7
Human adenoviruses (HAdV) and JC polyomaviruses (JCPyV) have been proposed as markers of fecal/urine contamination of human origin. An indirect immunofluorescence assay has been developed to quantify infectious human adenoviruses types 2 and 41 and JC polyomaviruses strain Mad-4 in water samples. The immunofluorescence assay was compared with other quantitative techniques used commonly such as plaque assay, tissue culture infectious dose-50 and quantitative PCR (qPCR). The immunofluorescence assays showed to be specific for the detection of infectious viruses, obtaining negative results when UV or heat-inactivated viruses were analyzed. The assays required less time and showed higher sensitivity for the detection of infectious viral particles than other cell culture techniques (1log10 more) evaluated. River water samples spiked previously with human adenoviruses and raw sewage samples were also analyzed using the proposed immunofluorescence assay as well as by qPCR. The results show quantitations with 2log10 reduction in the numbers of infectious viruses compared with the number of genome copies detected by qPCR. The immunofluorescence assay developed is fast, sensitive, specific, and a standardizable technique for the quantitation and detection of infectious viruses in water samples. © 2010 Elsevier B.V.
Rapid identification viruses from nasal pharyngeal aspirates in acute viral respiratory infections by RT-PCR and electrospray ionization mass spectrometryKuan Fu Chen | Richard E. Rothman | Padmini Ramachandran | Lawrence Blyn | Rangarajan Sampath | David J. Ecker | Alexandra Valsamakis | Charlotte A. Gaydos
Volume 173, Issue 1, April 2011, Pages 60-66
Diagnosis of the etiologic agent of respiratory viral infection relies traditionally on culture or antigen detection. This pilot evaluation compared performance characteristics of the RT-PCR and electrospray ionization mass spectrometry (RT-PCR/ESI-MS) platform to conventional virologic methods for identifying multiple clinically relevant respiratory viruses in nasopharyngeal aspirates. The RT-PCR/ESI-MS respiratory virus surveillance kit was designed to detect respiratory syncytial virus, influenza A and B, parainfluenza types 1-4, adenoviridae types A-F, coronaviridae, human bocavirus, and human metapneumovirus. Patients (. N=192) attending an emergency department during the 2007-2008 respiratory season consented, and " excess" frozen archived nasopharyngeal aspirates were analysed; 46 were positive by conventional virology and 69 by RT-PCR/ESI-MS, among which there were six samples with multiple viral pathogens detected. The sensitivity and specificity of the assay were 89.1% and 80.3%, respectively. Additional viruses that were not identified by conventional virology assays were detected (4 human bocaviruses and 7 coronaviruses). Samples in which the RT-PCR/ESI-MS results disagreed with conventional virology were sent for analysis by a third method using a commercial RT-PCR-based assay, which can identify viruses not detectable by conventional virologic procedures. Time to first result of RT-PCR/ESI-MS was 8. h. RT-PCR/ESI-MS demonstrated capacity to detect respiratory viruses identifiable and unidentifiable by conventional methods rapidly. © 2011 Elsevier B.V.
A real time RT-PCR assay for the specific detection of Peste des petits ruminants virusCarrie A. Batten | Ashley C. Banyard | Donald P. King | Mark R. Henstock | Lorraine Edwards | Anna Sanders | Hubert Buczkowski | Chris C L Oura | Tom Barrett
Volume 171, Issue 2, February 2011, Pages 401-404
Peste des petits ruminants virus (PPRV) causes a devastating disease of small ruminants present across much of Africa and Asia. Recent surveillance activities and phylogenetic analyses have suggested that the virus is an emerging problem as it is now being detected in areas previously free of the disease. As such, the virus not only is threatening small ruminant production and agricultural stability in the developing world, but also poses an economic threat to livestock in the European Union (EU) through introduction from European Turkey and North Africa. This report describes the development of a high throughput, rapid, real time RT-PCR method for the sensitive and specific detection of PPRV using robotic RNA extraction. This assay targets the nucleocapsid (N) gene of PPRV and has been shown to detect all four genetic lineages of PPRV in tissues, ocular and nasal swabs and blood samples collected in the field. The lowest detection limit achieved was approximately 10 genome copies/reaction, making this assay an ideal tool for the sensitive and rapid detection of PPRV in diagnostic laboratories. © 2010 Elsevier B.V.
Real time PCR method for simultaneous detection, quantitation and differentiation of capripoxvirusesCharles Euloge Lamien | Mamadou Lelenta | Wilfried Goger | Roland Silber | Eeva Tuppurainen | Mirta Matijevic | Antony George Luckins | Adama Diallo
Volume 171, Issue 1, January 2011, Pages 134-140
The genus Capripoxvirus (CaPV) comprises three members namely, sheep poxvirus (SPPV.), goat poxvirus (GTPV) and lumpy skin disease virus (LSDV) affecting sheep, goats and cattle, respectively. CaPV infections produce similar symptoms in sheep and goats, and the three viruses cannot be distinguished serologically. Since there are conflicting opinions regarding the host specificity of CaPVs, particularly for goatpox and sheeppox viruses, the development of rapid genotyping tools will facilitate more accurate disease diagnosis and surveillance for better management of capripox outbreaks. This paper describes a species-specific, real time polymerase chain reaction (PCR), based on unique molecular markers that were found in the G-protein-coupled chemokine receptor (GPCR) gene sequences of CaPVs, that uses dual hybridization probes for their simultaneous detection, quantitation and genotyping. The assay can differentiate between CaPV strains based on differences in the melting point temperature (Tm) obtained after fluorescence melting curve analysis (FMCA). It is highly sensitive and presents low intra- and inter-run variation. This real time PCR assay will make a significant contribution to CaPV diagnosis and to the better understanding of the epidemiology of CaPVs by enabling rapid genotyping and gene-based classification of viral strains and unequivocal identification of isolates. © 2010 Elsevier B.V.
A simple method for the parallel deep sequencing of full influenza A genomesMarie Louise Kampmann | Sarah L. Fordyce | María C. Ávila-Arcos | Morten Rasmussen | Eske Willerslev | Lars P. Nielsen | M. Thomas P Gilbert
Volume 178, Issues 1-2, December 2011, Pages 243-248
Given the major threat of influenza A to human and animal health, and its ability to evolve rapidly through mutation and reassortment, tools that enable its timely characterization are necessary to help monitor its evolution and spread. For this purpose, deep sequencing can be a very valuable tool. This study reports a comprehensive method that enables deep sequencing of the complete genomes of influenza A subtypes using the Illumina Genome Analyzer IIx (GAIIx). By using this method, the complete genomes of nine viruses were sequenced in parallel, representing the 2009 pandemic H1N1 virus, H5N1 virus from human and H1N1 virus from swine, on a single lane of a GAIIx flow cell to an average depth of 122-fold. This technique can be applied to cultivated and uncultivated virus. © 2011 Elsevier B.V.
Critical studies on binding-based RT-PCR detection of infectious NorovirusesDan Li | Leen Baert | Els Van Coillie | Mieke Uyttendaele
Volume 177, Issue 2, November 2011, Pages 153-159
Attempts were made to discriminate between infectious and non-infectious Noroviruses (NoVs) based on their viral binding properties followed by reverse transcription polymerase chain reaction (RT-PCR). Murine norovirus-1 (MNV-1) was employed as a surrogate to test the principle. Detection of both infectious and inactivated MNV-1 was investigated by the plaque assay, RT-PCR and binding-based RT-PCRs. The cell line RAW 264.7 and the ganglioside GD1a were used as binding receptors respectively in combination with RT-PCR. In the second stage of testing, similar approaches were applied to the two main genogroups of human NoVs (GI and GII). Differentiated Caco-2 cells and pig gastric mucin were tested as the binding receptors. Bovine serum albumin (BSA) was used as a non-specific binding control. In this study, the binding-based RT-PCRs decreased the detection of non-infectious NoVs by 1-3-log 10 while all infectious viral particles were detected. No significant difference was observed between the binding-based RT-PCRs within the concentration range investigated, except the binding level of human NoVs GII to pig gastric mucin was higher than to differentiated Caco-2 cells and BSA. This study indicates an improvement in the evaluation of the infectivity of non-cultivable human NoVs. This is also a comprehensive study on both specific and non-specific binding properties of NoVs. © 2011 Elsevier B.V.
Detection, characterization and quantitation of Coxsackievirus A16 using polyclonal antibodies against recombinant capsid subunit proteinsQingwei Liu | Zhiqiang Ku | Yicun Cai | Bing Sun | Qibin Leng | Zhong Huang
Volume 173, Issue 1, April 2011, Pages 115-120
Coxsackievirus A16 (CVA16), together with enterovirus type 71 (EV71), is responsible for most cases of hand, foot and mouth disease (HFMD) worldwide. Recent findings suggest that the recombination between CVA16 and EV71, and co-circulation of these two viruses may have contributed to the increase of HFMD cases in China over the past few years. Thus, for CVA16, further understanding of its virology, epidemiology and development of diagnostic tests and vaccines are of importance. The present study aimed to develop reagents and protocols for the detection, characterization and quantitation of CVA16. Recombinant CVA16 capsid subunit proteins VP0, VP3 and truncated VP1, were produced in Escherichia coli and used to immunize guinea pigs to generate polyclonal antibodies. The resultant three antisera detected specifically CVA16 propagated in Vero cells by immunostaining, ELISA and Western blotting. The antisera was used to show that CVA16 capsids were composed of correctly processed VP0, VP1 and VP3 subunits, and were present in the form of efficiently assembled particles. A method for the quantitation of the yield of CVA16 in Vero cells was established based on a Western blotting protocol using the recombinant VP0 as a reference standard and anti-VP0 as the detection antibody. This study shows the development and validation of reagents and methods, for qualitative and quantitative determination of CVA16, which are essential for the development of vaccines. © 2011 Elsevier B.V.
Comparison of hemagglutination inhibition assay, an ELISA-based micro-neutralization assay and colorimetric microneutralization assay to detect antibody responses to vaccination against influenza A H1N1 2009 virusS. Grund | O. Adams | S. Wählisch | B. Schweiger
Volume 171, Issue 2, February 2011, Pages 369-373
The hemagglutination inhibition (HI) assay has been the main method used to investigate immune responses to vaccination against influenza H1N1 (2009) virus. However microneutralization tests (MNT) have been shown to be more sensitive and more specific. In this study, the three methods of choice: (i) the HI assay, (ii) an ELISA-based conventional MNT and (iii) a colorimetric MNT in terms of their ability to detect antibody responses in serum pairs collected from 43 healthy individuals before and 21 days after vaccination were compared. The colorimetric MNT was established yielding intra- and inter-run imprecisions of 7.5% and 12.4%, respectively. Testing of antisera to seasonal influenza viruses demonstrated the assay to be specific for antibodies to influenza H1N1 (2009) virus. A good correlation between the three methods was found, being highest for the ELISA-MNT and the colorimetric MNT (r=0.714 for geometric mean titers (GMT) and r=0.695 for titer increases). Similar rates of fourfold titer increases were detected: 95.3% in the ELISA-MNT vs. 93.0% in colorimetric MNT and 95.3% in HI assay. The ELISA-based MNT demonstrated the highest titer range leading to the highest postvaccination GMT and the highest titer increase (>50-fold). The lowest GMTs were measured with the HI assay, while the colormetric MNT detected the highest GMT in prevaccination sera. Taken together, similar seroconversion rates were obtained with the three assays. The ELISA-MNT appeared to be the best method to compare absolute pre- and postvaccination GMTs. The colorimetric MNT, being less labour-intensive than the ELISA-MNT, seems to be a suitable tool in vaccination studies. © 2010 Elsevier B.V.
Poliovirus serotype-specific VP1 sequencing primersDavid R. Kilpatrick | Jane C. Iber | Qi Chen | Karen Ching | Su Ju Yang | Lina De | Mark D. Mandelbaum | Brian Emery | Ray Campagnoli | Cara C. Burns | Olen Kew
Volume 174, Issues 1-2, June 2011, Pages 128-130
The Global Polio Laboratory Network routinely uses poliovirus-specific PCR primers and probes to determine the serotype and genotype of poliovirus isolates obtained as part of global poliovirus surveillance. To provide detailed molecular epidemiologic information, poliovirus isolates are further characterized by sequencing the ∼900-nucleotide region encoding the major capsid protein, VP1. It is difficult to obtain quality sequence information when clinical or environmental samples contain poliovirus mixtures. As an alternative to conventional methods for resolving poliovirus mixtures, sets of serotype-specific primers were developed for amplifying and sequencing the VP1 regions of individual components of mixed populations of vaccine-vaccine, vaccine-wild, and wild-wild polioviruses. © 2011.
Simultaneous virus-specific detection of the two cassava brown streak-associated viruses by RT-PCR reveals wide distribution in East Africa, mixed infections, and infections in Manihot glazioviiD. R. Mbanzibwa | Y. P. Tian | A. K. Tugume | S. B. Mukasa | F. Tairo | S. Kyamanywa | A. Kullaya | J. P T Valkonen
Volume 171, Issue 2, February 2011, Pages 394-400
The expanding cassava brown streak disease (CBSD) epidemic in East Africa is caused by two ipomoviruses (genus Ipomovirus; Potyviridae), namely, Cassava brown streak virus (CBSV), and Ugandan cassava brown streak virus (UCBSV) that was described recently. A reverse transcription polymerase chain reaction (RT-PCR) based diagnostic method was developed in this study for simultaneous virus-specific detection of the two viruses. Results showed that CBSV and UCBSV are distributed widely in the highlands (>1000. m above the sea level) of the Lake Victoria zone in Uganda and Tanzania and also in the Indian Ocean costal lowlands of Tanzania. Isolates of UCBSV from the Lake Victoria zone were placed to two phylogenetic clusters in accordance with their origin in Uganda or Tanzania, respectively. Mixed infections with CBSV and UCBSV were detected in many cassava plants in the areas surveyed. CBSV was also detected in the perennial species Manihot glaziovii (DNA-barcoded in this study) in Tanzania, which revealed the first virus reservoir other than cassava. The method for detection of CBSV and UCBSV described in this study has important applications for plant quarantine, resistance breeding of cassava, and studies on epidemiology and control of CBSD in East Africa. © 2010 Elsevier B.V.
A universal real-time assay for the detection of LyssavirusesDavid T S Hayman | Ashley C. Banyard | Philip R. Wakeley | Graeme Harkess | Denise Marston | J. L N Wood | Andrew A. Cunningham | Anthony R. Fooks
Volume 177, Issue 1, October 2011, Pages 87-93
Rabies virus (RABV) is enzootic throughout most of the world. It is now widely accepted that RABV had its origins in bats. Ten of the 11 Lyssavirus species recognised, including RABV, have been isolated from bats. There is, however, a lack of understanding regarding both the ecology and host reservoirs of Lyssaviruses. A real-time PCR assay for the detection of all Lyssaviruses using universal primers would be beneficial for Lyssavirus surveillance. It was shown that using SYBR ® Green, a universal real-time PCR primer pair previously demonstrated to detect European bat Lyssaviruses 1 and 2, and RABV, was able to detect reverse transcribed RNA for each of the seven virus species available to us. Target sequences of bat derived virus species unavailable for analysis were synthesized to produce oligonucleotides. Lagos Bat-, Duvenhage- and Mokola virus full nucleoprotein gene clones enabled a limit of 5-50 plasmid copies to be detected. Five copies of each of the synthetic DNA oligonucleotides of Aravan-, Khujand-, Irkut-, West Caucasian bat- and Shimoni bat virus were detected. The single universal primer pair was therefore able to detect each of the most divergent known Lyssaviruses with great sensitivity. © 2011 Elsevier B.V.
New methods for the concentration of viruses from urban sewage using quantitative PCRByron Calgua | Jesus Rodriguez-Manzano | Ayalkibet Hundesa | Esther Suñen | Miquel Calvo | Sílvia Bofill-Mas | Rosina Girones
Volume 187, Issue 2, February 2013, Pages 215-221
Viruses are among the most important pathogens present in water contaminated with feces or urine and represent a serious risk to human health. Four procedures for concentrating viruses from sewage have been compared in this work, three of which were developed in the present study. Viruses were quantified using PCR techniques. According to statistical analysis and the sensitivity to detect human adenoviruses (HAdV), JC polyomaviruses (JCPyV) and noroviruses genogroup II (NoV GGII): (i) a new procedure (elution and skimmed-milk flocculation procedure (ESMP)) based on the elution of the viruses with glycine-alkaline buffer followed by organic flocculation with skimmed-milk was found to be the most efficient method when compared to (ii) ultrafiltration and glycine-alkaline elution, (iii) a lyophilization-based method and (iv) ultracentrifugation and glycine-alkaline elution. Through the analysis of replicate sewage samples, ESMP showed reproducible results with a coefficient of variation (CV) of 16% for HAdV, 12% for JCPyV and 17% for NoV GGII. Using spiked samples, the viral recoveries were estimated at 30-95% for HAdV, 55-90% for JCPyV and 45-50% for NoV GGII. ESMP was validated in a field study using twelve 24-h composite sewage samples collected in an urban sewage treatment plant in the North of Spain that reported 100% positive samples with mean values of HAdV, JCPyV and NoV GGII similar to those observed in other studies. Although all of the methods compared in this work yield consistently high values of virus detection and recovery in urban sewage, some require expensive laboratory equipment. ESMP is an effective low-cost procedure which allows a large number of samples to be processed simultaneously and is easily standardizable for its performance in a routine laboratory working in water monitoring. Moreover, in the present study, a CV was applied and proposed as a parameter to evaluate and compare the methods for detecting viruses in sewage samples. © 2012 Elsevier B.V.
Evaluation of five rapid diagnostic kits for influenza A/B virusChi Hyun Cho | Mi Kyung Woo | Ju Yeon Kim | Seok Cheong | Chang Kyu Lee | SeongSoo A. An | Chae Seung Lim | Woo Joo Kim
Volume 187, Issue 1, January 2013, Pages 51-56
Influenza viruses cause seasonal epidemics associated with high morbidity and mortality. However, even during periods of epidemic prevalence, clinical diagnoses are problematic. Rapid diagnostic tests for the detection of pandemic influenza A/B virus are valuable for their ease of use. Many rapid influenza diagnostic kits were introduced recently in the Republic of Korea (ROK), including Directizen EZ Flu A and B (Becton Dickinson, Sparks, USA), Binax Now Influenza A/B antigen kit (Binax, Portland, USA), Genedia influenza Ag (Green Cross, Yongin, ROK), Humasis Influenza A/B antigen test (Humasis, Anyang, ROK), and SD Bioline rapid influenza kit (Standard Diagnostics, Yongin, ROK). The objective of this study was to evaluate the performance of these five rapid diagnostic kits. The results were compared with those of viral culture and reverse transcription (RT)-PCR. A total of 253 nasopharyngeal swabs were analyzed from 253 patients (influenza A, n=67; B, n=86; negative samples, n=100). The specimens were tested immediately by conventional influenza virus culture and RT-PCR, stored at -80°C, and tested using five rapid test kits. The performance of the five rapid tests kits varied with sensitivities between 71.0 and 82.1% and between 37.2 and 47.7% for detecting influenza A and B, respectively. For influenza A, the sensitivities of the Directizen EZ Flu A and B, Binax Now Influenza A/B antigen kit, Genedia influenza Ag, Humasis Influenza A/B antigen test, and SD Bioline rapid influenza kits were 82.1%, 71.0%, 76.1%, 79.1%, and 82.1%, respectively; those for influenza B were 40.7%, 37.2%, 40.7%, 41.8%, and 47.7%, respectively. The specificity of all rapid tests was 100%. Commercial influenza antigen detection assays are useful tools for the rapid diagnosis of influenza. However, confirmatory testing is always recommended. © 2012.


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Dakar Sex Guide advises where to find sex, working girls, prostitution, street hookers, brothels, red-light districts, sex shops, prostitutes, erotic massage parlors, strip clubs and escorts in Dakar, Senegal.
Dakar is the capital and largest city of Senegal and is often regarded as the cultural and commercial center of French-speaking West Africa. The metropolitan area is home to about 2.4 million people and lies on the end of the Cap Verte peninsula, which marks the westernmost point on the African mainland. Dakar is often the first stop in exploring French-speaking West Africa because it has good flight connections with Western Europe, whereas the other main French-speaking West African city, Abidjan, does not.
- 1 Sex Scene and Prostitution
- 2 Red Light Districts
- 3 Prostitutes and Sex Workers
- 4 Street Hookers
- 5 Strip Clubs & Lap Dance
- 6 Brothels
- 7 Erotic Massage Parlors
- 8 Live Sex Cams
- 9 Dating
- 10 Transsexuals / Shemales
- 11 Gay and Lesbian
- 12 Sexual Services for Women
- 13 Sex Shops and Adult Stores
- 14 Escort Services & Agencies
- 15 Sleeping
- 16 Stay Safe
- 17 See Also
Sex Scene and Prostitution
Prostitution in Senegal is legal and regulated. Prostitutes must be at least 21 years of age, register with the police, carry a valid sanitary card, and test negative for sexually transmitted infections. NGOs working with prostitutes claim that the police abuses prostitutes. Senegal is becoming a popular destination for Female sex tourism. In Senegal, prostitution has been legalised for decades. The official reason for this is to keep track of the health status of the prostitutes. But others say legalising prostitution is just a way of attracting tourists.
Red Light Districts
There is no window style red-light district in Dakar, Senegal. But there is still a lot of prostitutes, Check the prostitutes section below.
Prostitutes and Sex Workers
Touristic beaches full of company seeking locals. Plage Bel-Air, Yoff, Yenn, N'Gor, and Toubab Dialao. Try these you'll not be disappointed.
For men
You will find prostitutes in tourist favored restaurant by the beach or in the city. Good example is Ngor island. The small harbour where the boat come, is a perfect spot. When evening is coming so the beach bars are full of girls waiting for company. Just pick up one and take her with you. When sun goes down the bars closes doors and nothing is happening in the until next morning.
For women
Just go any restaurant or to the beach. It is impossible to even avoid those men who are approaching you. There large selection of guys. From fake Rasta men smoking weed to real athletic guys showing of and makin pull ups on the beach. For woman it is more than easy. Just buy them dinner or something and they will take care of you.
Street Hookers
Most of the "locals" (mostly from neighboring countries) are seeking for company. They come out to the streets around three to four. Places where tourist like to hangout, restaurants, bars and avenues are filled of girls. There is no specific street for girls, but the quality girls are rooming in touristic areas. If you see a girl gazing you in the eyes so go for it, she is looking for a customer.
Les Almadies Dakar is the place to find gold diggers. Don't be surprise if you pay for a Coca-Cola 6euros. Girls are amazing tall, skinny the model type. On weekends the local rich family girl are around having fun these family girls are not looking for money they are very wealthy. Try La Patio or Duplex Club.
Strip Clubs & Lap Dance
Taxi drivers and bartenders will give you the directions to Brothels in Dakar. Remember Brothels are illegal in Senegal.
For example in Africa Star is really good for men seeking women. I went in ordered a beer and before i seat there was one Gambian girl asking if she can join me.
Erotic Massage Parlors
Most have 10,000 XOF promos you can work it from there for BJ's and HJ's. These massage parlors in dakar you can also call to you hotel room.
- Calizen massage 33 860 88 08.
- Sweetness massage 33 864 17 16.
- Royal Massage 30 103 12 72.
- Ideal massage 77 686 44 46.
- Millennium massage 77 316 85 82.
Live Sex Cams
Can I watch Live Cams in Dakar? - Yes, you can! Basically you can watch our online live sex shows for free anywhere in the world if you're connected to internet. You can choose any girl from our trusted Live Sex Cams! Join to get 10 free private teasers and 9.99 credits for free!
Live Sex Cams are booming at the moment. Webcam models can be found from all over the world and you can find girls, guys, trannies, couples and groups of different ages performing to you. Watching live sex shows is usually free, but if you buy some credits, you will have much better chances to see adult action as desired! It's possible also to hire a webcam model to a private chat room where you can ask the person to fulfill your fantasies.
Dating in Dakar can be a bit tricky due to cultural differences, so your best bet is to sign up for a dating site before your trip so you can meet a few locals ahead of time. AdultFriendFinder.com, for example, lets you meet local members in Dakar and get to know them on a personal basis before you arrive. Take advantage of features like live chat rooms and member webcams so you know who you are chatting with before arranging a face-to-face meeting. Since time in your destination may be limited, get to know each other's desires beforehand so when you do meet, you can skip the awkward introductions and start having some real fun.
Transsexuals / Shemales
Thousands of trannies all around the world are making their living by acting as a webcam model. You can watch transsexual live sex shows also in Dakar as long as you have internet connection.
Check it out: Tranny Live Sex Video Chat
Gay and Lesbian
Gay modeling in internet is getting more popular all the time and it's a big market alongside with gay porn. You can watch live gay sex shows also in Dakar as long as you have internet connection.
Check it out: Gay Live Sex Video Chat Senegal specifically outlaws same-sex sexual acts, and has prosecuted men accused of homosexuality in the past. Homosexuals face routine discrimination in the society.
Lesbian, gay, bisexual, and transgender (LGBT) persons in Senegal face legal challenges not experienced by non-LGBT residents. Senegal specifically outlaws same-sex sexual acts and, in the past, has prosecuted men accused of homosexuality. LGBT persons face routine discrimination in society.
All this does not mean that there are no gay scene. It is just hidden. Try Hotel Teranga if your seeking for gay company.
Sexual Services for Women
Senegal's gigolos actually have no nickname. But, according to a source quoted in a 2010 Globalpost report, female tourists target "rastas" who play the djembes (drums), because drummers are good in bed. Some observers brand the Senegal scene as male prostitution. Others see it as a harmless commercial take on dating.
The deal is a woman hits up with someone half her age and gives that new friend an all-expenses-paid ride in exchange for sex and a new lease of life. The Senegal gigolos apparently see themselves as tour guides who offer extras: a familiar pattern that puts the tour industry in a new light.
Sex Shops and Adult Stores
If you don't feel like visiting or cannot find any local sex shops in Dakar, you can easily order adult products from Online Sex Shop. Most pharmacies in Dakar will sell Viagra, Kamagra and Cialis without prescription. Some street hustlers will also sell some potency medicines, but you can never be sure if they are legit or not.
Escort Services & Agencies
You can find plenty of escort agencies online.
There is a large selection of hotels around Dakar. Most of the tourists stay in resorts and beach hotels. Anyway most of the hotels even assume that you will bring a companion with you. Many of them ask you when your checking in that are you going to bring company.
Stay Safe
Petty crime in Dakar is relatively high; crime against tourists is common, even around Place de l'Independance. Use common sense: women should not walk around alone after dark. Watch your pockets in crowded places, such as Sandaga, and keep a close eye on your belongings. There are many different scams to get money from tourists, so be wary. The African favourite 'I work at your hotel and have run out of fuel, please can I borrow €10' etc are common, so don't be fooled.
Crime around the airport is extremely high. Do not change money at the airport. Con artists wait inside the arrival terminal by the baggage carousel. They inform you that they work for the airport and that they do not need any money from you. They snatch your bags out your hands, and lead you to a restaurant where you are told the kitchen is closed. They will all sit around you and demand money before they let you or your bags go. Some may offer to take you to "an affordable hotel" such as Hotel Tahiti. They then lock themselves in the room with you and demand money. They do not settle for anything less than $US10-25. They do not care if you have enough money for food or to get back to the airport. They will threaten you. Be warned.
See Also
- Africa, Senegal
- Age of consent in Senegal
- Prostitution, Street prostitution, Global prostitution prices
- Sex topics & Phrasebooks, Sex worker, Prostitute types
- Red-light district, List of red-light districts all over the world
- Brothel, Escort agency, Call girl, Erotic massage, Strip club
- Sex tourism, Sex industry, Countries with most prostitutes
- Gay, Lesbian, Gay and lesbian travel, Bisexuality, Ladyboy
- Age of consent, Stay safe, Scams, Safe sex, STD, HIV/AIDS


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Raise It Up! pays homage to J Dilla this Saturday at Stonefly
Today marks the birthday of one of hip-hop music's greatest producers, Detroit's J Dilla. His life was cut short three days after his 32nd birthday due to a rare blood disorder. Dilla left behind several classic albums, songs and beats that most rappers would give a limb to rap over.
Video: The Concert Corner - Recapping Kenny Chesney
Country superstar Kenny Chesney performed for a sold-out crowd at Miller Park Saturday for the Brewers ballpark's first major concert since 2010. Photo by Rick Wood. »Read Full Blog Post
CBS pulls 'Mike & Molly' tornado-themed finale
CBS has pulled Monday night's "Mike & Molly" season finale in light of today's tornado devastation in Oklahoma, according to TheWrap.com.
The episode, entitled "Windy City," placed the characters in a tornado in Chicago. »Read Full Blog Post(1)
Summer Reading: Books for people who loved John Green's 'The Fault in Our Stars'
On May 26, we're publishing our Summer Reading preview in the Journal Sentinel's Sunday Cue section and at TapMilwaukee.com, with dozens of suggestions for your enjoyment.
For today's preview of those suggestions, we asked our friends at Boswell Book Company to suggest young-adult titles for readers who were captivated by John Green's novel "The Fault in Our Stars," the story of two teenagers who meet at a cancer support group and fall in love. Booksellers Jannis Mindel, Nick Berg, Stacie Michelle Williams, Hannah Johnson-Breimeier and Daniel Goldin suggest these young-adult novels for readers who loved "Fault." »Read Full Blog Post
Ryan Braun, Aaron Rodgers opening Bayshore restaurant
8-Twelve MVP Bar and Grill, a restaurant associated with athletes Ryan Braun and Aaron Rodgers, will open its second location at Glendale's Bayshore Town Center.
The new 8-Twelve will be located in the space now leased to COA, a locally owned Mexican restaurant which Marc and Marta Bianchini opened in 2009. COA closes June 30, and 8-Twelve is expected to open in the fourth quarter, said David Moss, Bayshore general manager. »Read Full Blog Post(23)
'Summertime': 'The livin' is easy,' in many different voices
'Summertime,' the signature lullaby from 'Porgy & Bess,' is one of America's greatest musical hits. More than 33,000 versions of the song have been recorded, according to The Summertime Connection, a Dutch website that compiles a list of recordings of the song.
Through June 9, Milwaukee audiences can hear the song performed live at the Broadway Theatre Center, where the Skylight Music Theatre is staging "Porgy & Bess," with Kearstin Piper Brown and Rhea Olivaccé alternating in the role of Bess. »Read Full Blog Post(1)
Pabst Mansion hosts summer wine tasting
The Pabst Mansion, 2000 W Wisconsin Ave., hosts a summer wine tasting on the mansion's front terrace June 14.
Downer Avenue Wine will select the wine list and Buddy Squirrel will provide Milwaukee-made chocolates. »Read Full Article
GMC and MiKE get $350,000 ArtPlace grant
ArtPlace America, a consortium of national and regional foundations and banks that works with the National Endowment for the Arts and other federal agencies, will award a $350,000 grant to the Greater Milwaukee Committee's MiKE initiative.
On Tap Today: Drink up, me hearties - while you still can
Summer's finally here, but some things are leaving town. One of them is the "Real Pirates" exhibit at the Milwaukee Public Museum, which pulls out next Monday. So, if you haven't set sail for it yet, you might want to heave to.
Speaking of scoundrels, the Brewers limp home today to start a three-game series against the Los Angeles Dodgers. Maybe the team's "Spring Madness" ticket-deal promotion will help fill Miller Park - and get the team back in a winning frame of mind. »Read Full Blog Post


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Largemouth Bass fishing should be good in 2016. Sampling data from 2014 indicated that approximately 33 percent of bass were greater than 15 inches and 66 percent were above 12 inches. In addition, as a result of the high water levels throughout the spring and summer of 2013, there is a large year class of fish that will be in the 12-15 inch range this year. That year class should provide good bass fishing in the years to come. A 12-15 inch protected slot length limit helps provide excellent catch-and-release fishing on Fellows Lake.
Muskellunge sampling was conducted in March of 2015. Based on sample data, muskie fishing at Fellows should be good in 2016. Data from last year indicated that 32 percent of the population is greater than 36 inches (legal harvest size) and 19 percent of the population exceeds 40 inches. This is the highest percentage of fish over 40 inches observed in a muskie survey on Fellows Lake since 2004. The largest fish observed during last year's sampling was 44.8 inches. Show-Me Muskie Program anglers fishing for muskies in 2014 encountered a muskie every 4.5 hours and it took anglers 21.6 hours of fishing to catch a muskie 36" or longer. This was the lowest number of hours needed to catch a legal fish in the state in 2014. Sample data and fishing reports create a positive outlook for muskie fishing in 2016, as the populations seems to have recovered from the high water temperatures and extremely low lake levels during the 2012 drought. Expect best muskie fishing success on overcast, rainy days during the fall or early spring months when water temperature is below 70 degrees F. Fish large spinners, bucktails, or crank baits near brush structure or weed beds. Muskies are occasionally caught as anglers fish for largemouth bass or walleye. Careful handling of both sub-legal and legal muskie at the water's surface will increase their chances for survival. Fishing for muskie during summer months when water temperature is warmer, will likely decrease the chance for survival of released muskie.
Channel catfish remain abundant throughout the lake and range from 10-20 inches. Preferred baits include chicken livers, nightcrawlers, prepared baits, and cut bait. Walleye and crappie anglers should expect fair fishing success. Walleye can be caught fishing gravel points in the spring, the backs of coves in the summer using crappie jigs, nightcrawlers, or minnows, and trolling deep-diving crankbaits across main lake points. The minimum length limit for walleye is 15 inches. Minnows and tube jigs are the preferred baits for crappie anglers in and around brush piles and vegetation. Both white crappie and black crappie are present with most fish ranging from 8-11 inches. Crappie greater than 14 inches occasionally provide anglers with a memorable catch. Bluegill fishing will be fair this year, but most will range from 4-6 inches. Sampling data from 2014 indicated that quality size bluegill (above 6 inches) make up about 20 percent of the population. Small pieces of worms and a bobber make an excellent rig for children to catch this species. Low densities of redear sunfish and white bass are also present in the lake.


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Basic iPhone / iPad App
This project received 8 bids from talented freelancers with an average bid price of $265 USD.Get free quotes for a project like this
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Project Budget$30 - $250 USD
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Project Description
This is a simple passcode app job for an app that will work on iPhone & iPad
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Final Four Does Involve College Basketball
The $1.3-billion venue, configured for 90,000 fans and flaunting a video board 66 feet longer than the basketball court, screams of mad excess. The ticket prices, the average salary at the coaches? convention, the lingering presence of Jerry Jones, the custom Lucchese cowboy boots and Stetson hats ? all of it reeks of luxury and affluence. The team receiving the most attention, Kentucky, is coached by a $5.4-million-a-year guerilla recruiter, John Calipari, who sells mercenary teenagers on the often-bogus dream of staying one year in school before leapfrogging to the NBA.
Does anything even remotely suggest ``college?? at the Final Four?
Why, yes. Bo Ryan does. Sitting beside Calipari at a news conference, the two struck a curiously antithetical pose, with Ryan representing all that is old-school and fundamental about basketball and a college education at Wisconsin. When the inevitable question was posed immediately ? the one-and-done emphasis at Kentucky vs. the four-and-done demand at Wisconsin, where Ryan has one of the highest graduation rates in the sport ? Ryan tried to lighten the mood. ``Well, here's all I got to say to Cal. When somebody asks me about one and done, all I remember is when my mom would give me a pork chop or a piece of meatloaf and I would ask for another piece and she would say, `No, one and done.? ?? Giggles aside, everyone is aware of the classic culture clash in this national semifinal, which, by no coincidence, is the featured game Saturday night. And Calipari, whose antennae are high and his senses battered over perceptions that he?s academically lax, instantly went into defensive mode about the way he runs his program.
``First of all, does a player have to be here four years to be a terrific college player??? he said. ``The last four years, our (collective) grade point average has been a 3.0. Our APR is as high as anybody in the country. They're college students; they're just not college students for four years, in most cases, but in some they are. We don't talk about it ? we don't talk about NBA. We're worried about winning college games and being a great college team. Losing yourself in the team, doing less which ends up being more, losing yourself in the game. So I don't think the kids are thinking all those things. The issue `one and done? has now become a bad connotation. So we're going to break out something new this week to get you guys off this `one and done? so that we can think about it in another term, which is trying to help these kids do what they're trying to do as college students, as where they want their careers to go.??
Who?s calling BS first?
From Derrick Rose at Memphis to John Wall, DeMarcus Cousins, Anthony Davis, Michael Kidd-Gilchrist, Julius Randle and others to come at Kentucky, what Calipari is doing is running an on-campus version of an NBA developmental league. Ryan, who got into the business as a junior high-school teacher who was given a coaching job as a sidelight, is trying to prepare players for life via the university curriculum. ``For me, I get so excited about seeing the student get an A on the test as much as when I see a player get better and perform well and come together and have a unit come together as a team,?? Ryan said.
Never has a Final Four game had such significant societal battle lines. It wasn?t designed as such by the NCAA selection committee ? honestly, who saw either team advancing this far two weeks ago? ? but now that it has materialized, it?s hard not to take a side. I have Wisconsin, which can prove it?s possible to get a four-year degree AND beat a future team of NBA players in one swoop. For that matter, the other two teams, Florida and Connecticut, also are closer right now to Wisconsin in structure and philosophy. The UConn star, Shabazz Napier, kept a promise to his mother that he would graduate as a senior, rejecting an early path to the NBA when the program was banned from the NCAA tournament last year. Florida?s Billy Donovan produces NBA players, for sure, but not until they?ve spent seasons ? plural ? in his program and he has taught them about hoops and life.
The basketball junkie in me has Kentucky and Florida meeting for the national championship. But the voice in my head is cheering hard for Ryan, who keeps bringing up Platteville. That would be the University of Wisconsin-Platteville, the Division III program where he coached before the big boys in Madison found him 13 years ago. ``I always promised my Platteville guys to stay true in those national championships that we had, when we played in front of maybe 5,000. So I don't know what it's like being in front of 75,000,?? he said. ``But once the ball is thrown up, you still have to manage in those first timeouts. No matter whether it's Division I or Division III, you got to manage emotions and energy and try to channel it the right way and get everybody concentrating on what it is we do and don't try to be somebody that we're not.
``When it comes time to play, then just be who you are.??
We know who Ryan is. We know who Calipari is.
May the more honorable method win.


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Average Ratings
1 out of 1 people recommended this car.
Consumer Reviews
reliable car
by Practical from Miami | December 8, 2010
The situacion is that the parts did not exist practically,but I had the car for 10 years and I would like to have another,like this,or a little better,but Huynday
Purchased a new car
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Does recommend this car
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Accounting and Audit Matters: Fundamentals
This session deals with current issues in accounting and auditing and seeks to improve directors' understanding of accounting principles (and related auditing standards) that are of particular current importance. What, for example, should the general-experience board member know about International Financial Reporting Standards (IFRS) and anticipated convergence between IFRS and United States' GAAP? The session's goal is to provide the "ordinary" board member—typically not a member of the audit committee, or possibly a newly-appointed member—with the general awareness of these issues that can assist in a better fundamental understanding and, therefore, better service to the Company
Roman Weil
V. Duane Rath Professor Emeritus of Accounting, The University of Chicago Booth School of Business
Advanced Topics In Compensation: Peer Groups, Say on Pay, and New Tools
In a departure from past practice, nearly every board member must now be sensitive to issues of compensation, have a certain level of knowledge and expertise not previously expected, and must be able to practically evaluate and contribute reasonable judgment about certain compensation decisions. This session will help new compensation committee members or other board members not typically involved in compensation committee matters develop a basic level of understanding about compensation obligations, best practices, and basic tools for navigating the increasingly complex arena of executive compensation. Topics covered will include an in-depth look at peer groups, say on pay, and other new tools, as well as guidance for evaluating the basic components of compensation, interacting with compensation consultants, and understanding key legal issues that impact compensation.
Shekhar Purohit, Managing Director and head of the California Office, Pearl Meyer & Partners
Scott Spector, Partner, Fenwick & West LLP
David Chun, CEO and Founder, Equilar
Building a Better Board: Diversity and Beyond
There is a growing urgency for boards to increase their numbers of women and minority directors. Several foreign countries already require that the boards of publicly traded firms have a minimum percentage of women directors. Boards are also perceiving the need to add novel skill sets relating to risk assessment, technology, and international marketing, and to consider term limits for directors. The objective is to move away from the traditional demographic description of boards as "pale, male, and stale." This session explores these imperatives, analyzes the evidence regarding the benefits of board diversity, and reviews pragmatic strategies that boards can adopt in response to calls for greater diversity.
Mary G. F. Bitterman, President, The Osher Foundation; Lead Director, The Bank of Hawaii
Mary Cranston, Retired Senior Partner, Pillsbury Winthrop Shaw Pittman LLP; Director, Catalyst
Daniel Kelly, Jr., Partner, Davis Polk & Wardwell LLP
Bonnie Gwin, Vice Chairman, Heidrick & Struggles
China: Friend, Enemy or Frenemy
China is perhaps America's most important and most challenging economic partner. Whether the issue is intellectual property protection, labor practices, cyber-security, pollution, foreign corrupt practices risks, or the quality of Chinese accounting statements, doing business in China presents a set of unique risks and opportunities that often require careful board attention. This session addresses board level practices that are appropriate for United States firms with substantial China exposure, with particular attention to recent developments suggesting that US regulatory authorities may be paying special attention to US business practices in China.
Alan R. Denenberg, Partner, Davis Polk & Wardwell LLP
C.Y. Wang, Americas Area Leader, China Business Network, Ernst & Young
Edward Westerman, Senior Managing Director, FTI Consulting
Ed Batts, Partner, DLA Piper
Corporate Governance and Proxy Trends
Shareholder activism is on the rise. "Say on pay," just-vote-no, majority voting, and shareholder proposals are among the many different measures that investors are using to influence boardroom behavior. Large activist investors are becoming more sophisticated in targeting companies and in deploying communication strategies designed to pressure boards of directors. This session will review the state of the art in shareholder activism and the broad array of strategies available to directors facing pressure from their shareholder base.
Susan Blount, Vice President and General Counsel, Prudential Financial
Peter Clapman, Chairman and President, Governance for Owners USA
Christopher Young, Head of Contested Situations, Credit Suisse
Richard Koppes, Administrative Officer, National Association of Public Pension Attorneys (NAPPA)
Crisis Anticipation and Control
A large number of corporate crises can be anticipated, and boards can help their corporations assure that they are optimally positioned to respond if and when the crisis arises. This session will explore the roles that boards should and should not play in the event of a corporate crisis, review the state of the art in crisis control techniques, and evaluate a range of case studies of successful and failed examples of crisis control.
Gidon Caine, Partner, Alston & Bird LLP
Don Keller, Partner, Center for Board Governance, PricewaterhouseCoopers
Marsha Simms, Partner, Weil, Gotshal & Manges (retired); Director, Sotheby's
Bob Malone, President & CEO, Bank of Sonora, Director, Peabody Energy Corporation; Director, Halliburton Company
Defend Your Company, Defend Yourself: Modern Litigation Strategies
This breakout session will focus on the fundamentals behind recent legal trends, liability concerns, and investigations that impact public corporations. We will discuss director liability and best practices as concerns board member responsibility and conduct. Panelists will discuss suits against corporate targets and issues of interest in light of new or reinvigorated SEC enforcement strategies. We will discuss the selection of litigation targets, the legal strategies used to exploit or defend director and corporate actions, and measures that directors can and should take in order to be prepared in today's litigation environment.
Jordan Eth, Partner, Morrison & Foerster LLP
Jessica Valenzuela Santamaria, Partner, Cooley LLP
Sara Brody, Partner, Sidley Austin LLP
Foreign Corrupt Practices Act (FCPA) and UK Anti-Bribery
The federal government continues to bring major anti-bribery prosecutions at a rapid pace. More than 115 FCPA actions have already been filed and more than 104 publicly traded companies have already disclosed that they are conducting internal investigations or being investigated by the federal government because of concerns regarding foreign bribery. The British government has also adopted anti-bribery provisions that potentially reach many US firms and that are broader than US law in several respects. Experience also teaches that internal investigations of potential FCPA violations can be exceedingly expensive and can quickly generate fees in the millions of dollars. This session reviews recent developments in the global effort to reduce corruption, the risks that the FCPA and British law pose to US firms, and rational steps that directors can adopt to help minimize FCPA risk or to address the risk once it is manifest.
Tamika Tremaglio, Partner, Deloitte Financial Advisory Services LLP
Susan Muck, Partner, Fenwick & West LLP
Alan Crain, Senior Vice President and General Counsel, Baker Hughes Incorporated
Lead Directors: Role and Function
OK–under pressure from ISS, we appointed a lead director. What does she do? What is the role of the lead director? Is it "a poor man's non-executive Chairman," or is it something different? Is there a model, or is it different things for different companies and boards? And for whom–and under what circumstances–is it important? Or is it just window dressing? The panel will examine these and related questions about the actual functioning of the lead director role in the modern corporation.
Lon Allan, Chairman Emeritus, Silicon Valley Directors' Exchange (SVDX)
Steve Alogna, Partner, Deloitte LLP
Joshua Ehrlich, Chairman, Global Leadership Council
Daniel Kelly, Jr., Partner, Davis Polk & Wardwell LLP
Innovation: A Guide to the Board's Role
It has been suggested that going public hurts the innovative process. If that's true, then a particular responsibility of the Board should be to encourage the constant innovation that all companies need to maintain, or improve, their competitive position. The panel will discuss approaches to that critical task.
Shai Bernstein, Assistant Professor of Finance, Stanford Graduate School of Business
Jeff Jordan, General Partner, Andreessen Horrowitz
Kate Mitchell, Managing Director, Scale Venture Partners
Evan Epstein, Executive Director, The Arthur and Toni Rembe Rock Center for Corporate Governance
Indemnification and D&O Insurance
Directors and Officers insurance coverage is an issue of huge importance for corporate boards and management. Our panel of industry experts will lead a discussion highlighting the most important terms and conditions of a D&O insurance policy. When and under what conditions, for instance, do insurance companies tend to contest payment on their policies? How does indemnification work to protect directors and officers during and after a suit, and what is the importance of advancement of legal fees to covered parties? Are there any new trends, such as a renewed focus on ERM (Enterprise Risk Management) that might impact future board liability and issues of coverage?
James Kramer, Partner, Orrick
Kevin LaCroix, Executive Vice President, Oakbridge Insurance Services; Author, The D&O Diary
Priya Cherian Huskins, Senior Vice President, Woodruff-Sawyer & Co.
Ombudsmen within Corporate America
Stanley Sporkin has pioneered the role of the ombudsman (ombudsperson?) as a resource that company employees can call on to counter improper behavior and minimize corporate risks. Does your company have one? Should it? The panel, including Judge Sporkin, will examine the relevant considerations.
Bob Malone, President & CEO, First National Bank of Sonora; Former CEO, BP America; Director, Peabody Energy and Halliburton Company
Stanley Sporkin, Former District Judge, United States District Court for the District of Columbia; Ombudsman, BP America
James Balassone, Executive-in-Residence Markkula Center for Applied Ethics, Santa Clara University
Political Activity, Lobbying and the Board
The Supreme Court's recent decision in Citizens United opens the door to far more active political participation by corporations. But should corporations become more actively involved in the political process, and if so, what are the most effective strategies available to the corporation? What role should the board play in monitoring corporate political activity? How can the board help assure that the corporation complies with all relevant disclosure and contribution limitation provisions? Estimates suggest that the upcoming presidential cycle will involve expenditures in excess of $1 billion by each major party, and state and congressional campaigns will only add to the total. This panel, composed of executives and advisors with extensive experience in the political process, will offer pragmatic advice about corporate involvement in the political process, whether through lobbying, direct contributions, activity with trade associations, or through other channels.
Alison Kutler, Head of Government Policy for the Americas, Visa
Senator Russ Feingold, former United States Senator
Rey Ramsey, former CEO, TechNet
Special Investigations
Special investigations—typically required when there is a suggestion of a serious legal violation or conflicts-of-interest within the company—have become a fact of life for most companies. If you serve as a director and haven't seen a special investigation take place, you will. When that happens, there are a number of difficult questions that will need to be addressed and that you will need to be prepared for. This session will cover questions such as at what stage an investigation should be initiated, who should conduct it, how the attorney-client privilege works in this context, and how the report of the investigation should be handled.
Thad Davis, Partner, Co-Chair, Securities Litigation, Gibson Dunn
Sarah Good, Partner, Pillsbury Winthrop Shaw Pittman LLP
Andy Vollmer, Partner, WilmerHale LLP


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This event has expired. This may be a recurring event, so please check the calendar in the future for updated occurrences.
All Schools Reunion
A potluck meal at noon begins the festivities, followed by an informal program which has in the past included displays of photos of the 12 rural school houses, a storyteller, and audience participation of shared memories. Sponsored by the Jump River Valley Historical Society.
Fair/Festival Type
- Community
Free Admission
- Yes


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5 People Injured in 6 Car CrashThe multiple-car accident happened at New Halls Ferry Road and North Lindbergh Boulevard at 10 p.m.
Real-Life Superhero 'Citizen Saint' Patrols North St. Louis County StreetsSelf-described "concerned citizen" spreads the message to keep lights on, bad guys out.
Police Arrest Six Men in Connection to Auto Repair Shop BurglariesA total of eight vehicles were stolen.
60 Firefighters Work to Extinguish Abandoned Warehouse FireThe two-alarm fire at N. 7th and O'Fallon Streets in St. Louis was burning on four levels of the five-story building.
Two St. Louis-Area Men Arrested Near World Trade CenterA police officer noticed that one of the men had a pistol in his waistband.
KMOX Profiles: Rev. Traci BlackmonThe shooting death of Michael Brown changed many things in our country, including the life of a pastor in the St. Louis County community. Rev. Traci Blackmon became active in the protest and dialogue around policing minority communities.
Florissant Fights Management Change for License OfficeThe Mayor of Florissant is calling on residents to make their voices heard concerning the future of the state license office in his city.
Protesters Want Florissant to End Ban on Pit BullsProtester Donna Slemmer says pit bulls ``are no more dangerous than Chihuahuas ...''
Halls Ferry Elementary in Florissant Closed Tuesday Due to FireHalls Ferry Elementary School in Florissant is closed Tuesday after a kiln overheated in an art closet and caught fire.
Florissant Council Considers Sweatshop BanThe proposal would require vendors certify their production lines are sweatshop-free before doing business with the city.
Bob Evans Locations Close NationwideBob Evans is closing 27 under-performing restaurants, including a location in Florissant.
Charges Filed in Litchfield to Florissant ChaseThe man who led police on a chase from Litchfield to Florissant has been charged.


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Available Colors
Exterior Colors
Bright Silver Metallic Clearcoat
Brilliant Black Crystal Pearlcoat
Inferno Red Crystal Pearlcoat
Modern Blue Pearlcoat
Stone White Clearcoat
Interior Colors
Dark Khaki/Light Graystone
Dark Slate Gray
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Following the trend of nearly million dollar townhomes in Queen Anne and Eastlake, here's one in Westlake at 2010 8th Ave N for $939k. It's a 3 bedroom, 3.25 bathroom, 2,760 square foot place. We dig the concrete floors, exposed beams, roof top deck and especially the built in grill and sink on the roof… Read More
Nearly $1m Westlake Townhome
May 30, 2014 By Filed Under: Westlake Views: 1,337 Leave a Comment
Killer Rooftop Deck Overlooking Lake Union
July 20, 2012 By Filed Under: Westlake Views: 3,090 Leave a Comment
I love this Pb Elemental NW contemporary townhouse at 2010 8th Ave N. $849k for 2,647 square foot, 3 beds, 3.5 baths. Looks like it sold for $1m back in 2006. What I love the most is the killer roof top deck. Not only does it have killer views but an amazing outdoor kitchen.


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Rev. Irene Monroe: To a nationwide community of same-gender loving (SGL), bisexual, transgender and progressive heterosexual African American men, Manago is the MAN!, seen as a visionary, game changer and "social architect."
Massachusetts' Throw-Away Kids
Rev. Irene Monroe: Homelessness among heterosexual students came in at 3.2 percent. This rate increased to 15 percent among bisexual students, and 25 percent among lesbian/gay students.
Our Bi-Phobia Placed on Sheryl Swoopes
Rev. Irene Monroe: My head spins at the thought of how Christian" de-gaying" counseling services, like Dr. Marcus Bachmann's, Michele Bachmann's spouse, could politicize Swoopes' seemingly sexual flip-flopping as their poster-child.
Re-introducing Lesbian, Bisexual, and Transgender Women of African Descent
Rev. Irene Monroe: After nearly two decades of LBT women of African descent's invisibility on a national level Zuna is causing a revolution by taking the bold step in this era of single-issue queer politics to remind us all we, too, matter.
Enough HDIB For Everyone; Heterosexual Intercourse Outlawed
There's enough hate, discrimination, injustice and bigotry (HDIB) generated against Americans of all stripes, that no one group can ever begin to absorb all of it. No need to covet another's HDIB. There's plenty to go 'round. A bountiful amount available to all.
King of Kings Is Not Judging King and King
It's getting really religious out there. I've just learned that Jesus Christ is against same-sex marriage and is promoting a yes vote on Prop 8. Prop 8 would amend California's constitution to take away the right of same-sex couples to get married. I found this out when I saw a full page ad in the […]
Black Media Fails Its LBGTQ Community
By now many in the LGBTQ community have heard of the news about the cop beat down of Duanna Johnson in a Memphis jailhouse that was captured on a surveillance video. Those of us especially of African descent, who don't know or haven't seen a photo of Johnson, might pick up on a cultural marker […]