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We've already seen how useful the Layers panel can be when identifying where objects appear inside the object hierarchy, and even how to change stacking order within a file without actually having to select the objects themselves. However, it's important to realize that we can do even more things through the Layers panel as well. And in this movie I wanted to discuss actually moving objects between layers, or even copying objects, and copying appearances as well. I'll click on the triangles here to reveal the contents of these layers. Now even when I am using layers inside of a document, it's extremely rare that I would actually create a layer first and then add artwork to that layer.
Usually I work in the opposite direction. I just add a whole bunch of artwork to my document. I just don't want anything to get in the way of my creative process. And then when I kind of step back for a moment and I realize I really need some kind of structure inside of my document, at that time I may go ahead and start creating layers and move artwork into their respective layers. For example, in this case I already have some artwork in my document, inside of this layers 2 file, but I may decide that I want to put all the text elements on their own layer. So right now I have here this Directions text, and I have the MAIN STREET text, and the TRAIN STATION text.
So I'll start by actually creating a new layer. I am going to double-click on that layer, and I'll give it a name. Let's call that one Labels for now. Now if I wanted to select all the text elements, I could go over here on the right side and start clicking over here and holding down the Shift key to do this. But I want to show you yet another way to make selections in a little bit more of an efficient manner. So I'll just click over here to deselect everything, and I'll go up over here to the Select menu inside of Illustrator and I'll choose object. I want to select certain types of objects. Since I want to work with my text objects here, I can choose Select > Object > Text Objects, and now all the text elements in my file become selected.
You'll notice, by the way, that the Hansel & Petal text there was not selected because that text has already being converted to outlines. So it's no longer text, those are actually regular paths. So let's now focus on the little squares that I see on the right side of the Layers panel. Remember that the squares refer to selection, meaning they refer to structure, the paths themselves. If I want to move the structure, I want to move the elements onto a completely different layer, I could simply grab this little square right here and drag it into the Labels layer.
And if I release the mouse, I've now successfully moved those three elements into that Labels layer. So the boxes here don't only serve as a way for me to visualize which layers objects belong to, I can actually drag those objects, or those little boxes, up and down to move them within the object hierarchy-- in this case, from one layer to another. I am going to press Undo for a second here, because maybe I want to keep the text on this layer but I want to create a copy of these elements on the Labels layer. So now I am going to do the same thing. I am going to just click and drag on this little box right here and start to drag upwards, but in doing so, I am going to hold down the Option key on my keyboard.
Option, as you're dragging something on a Mac, will duplicate something, and if you're on Windows, you would hold on the Alt key to perform the same function. I am simply going to hold the Option key and drag now into the Labels layer, and that's now going to not just move the elements, but copy the elements into the Labels layer. Notice, by the way, that when I created a new layer, Illustrator automatically selected a new color, and you can easily see right now that that color now is being shown as well. And I am going to turn off the Labels layer here for a moment, because what we just did was we actually moved some structure around inside of our document.
We physically moved paths from one layer into another. You know, I know that sometimes people will actually, like, select artwork, go to a different layer and then paste. There's really no need to do that all. Simply move those little dots up and down inside of your Layers panel. Alternatively, you can actually hold down the Shift key and select multiple layers. Or if you want to select noncontiguous layers, press the Command--or if you're on Windows, the Ctrl--key to select noncontiguous layers, and then you can move those elements into other layers as well.
But let's say you actually want to move or copy an appearance. We've just has been moving structure now by moving those little squares, but inside of Illustrator you can also move or copy appearances. For example, this Train Tracks looks pretty cool, and maybe I want to use that as a border for something. Like if we take a look at this box here, for example, that indicates the building. Maybe I want to create some other kind of a design, so I may want to actually apply the Train Tracks of this rectangle as well. I can do that pretty easily directly from the Layers panel without even having to select any artwork in my document.
I am going to click on the triangle here to reveal the contents of the Store group, and over here I can see that shape which is called Building. I want to take the appearance that's applied to the train tracks and I actually want to apply that appearance to the building as well. We've already discussed the ways inside of Illustrator like for example, using graphic styles or the Eyedropper tool. But this is yet another method that I can do this. I am simply going to click on the target circle itself, and I'll do this on the Train Tracks object. I'll hold down the Option key--or if you're in Windows, the Alt key--and I'll drag now down onto the building.
You can see now how I have a basic appearance on building, but as I am dragging down I can now apply that filled circle--that meatball, if you will--and now I release the mouse and you can see that the train tracks now appears on that object. I'll press Undo because I really want it to look just like a regular, plain rectangle right now. But you get the point: inside of Illustrator using the Layers panel, I can easily move objects between different layers or between different groups, and I can also use the actual target circles themselves to copy appearances from one object to another.
- Targeting individual object attributes
- Adding multiple stroke and fill attributes
- Modifying appearances with live effects
- Applying effects to groups and to layers
- Understanding both selecting and targeting
- Copying artwork and appearances from layers
- Using the Outline Object effect
- Enhancing performance with the Rasterize effect
- Creating quick and easy captions and buttons
- Setting up a meaningful workspace
- Controlling the pixel resolution of effects


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Job openings rate up slightly in December 2004
February 10, 2005
On the last business day of December 2004, there were 3.4 million job openings in the United States and the job openings rate was 2.5 percent. In November the job openings rate was 2.4 percent.
The job openings rate has generally trended upward since August 2003, when the rate was 2.0 percent.
In December 2004, the job openings rate increased for private industries overall, and for the professional and business services and leisure and hospitality industries. The job openings rate rose in the South region, but showed little or no change in the other regions of the country.
The job openings rate is the number of openings divided by employment plus job openings. A job opening requires that a specific position exists and there is work available for that position, work could start within 30 days regardless of whether a suitable candidate is found, and the employer is actively recruiting from outside the establishment to fill the position.
These data come from the Job Openings and Labor Turnover Survey. The above data are seasonally adjusted. Data for December 2004 are preliminary and subject to revision. Find additional information in "Job Openings and Labor Turnover: December 2004" (PDF) (TXT), USDL 05-206.
Bureau of Labor Statistics, U.S. Department of Labor, The Economics Daily, Job openings rate up slightly in December 2004 on the Internet at http://www.bls.gov/opub/ted/2005/feb/wk1/art04.htm (visited September 30, 2016).
Recent editions of Spotlight on Statistics
A look at healthcare spending, employment, pay, benefits, and prices
As one of the largest U.S. industries, healthcare is steadily growing to meet the needs of an increasing population with an increasing life expectancy. This Spotlight looks at how much people spend on healthcare, current and projected employment in the industry, employer-provided healthcare benefits, healthcare prices, and pay for workers in healthcare occupations.
Employment and Wages in Healthcare Occupations
Healthcare occupations are a significant percentage of U.S. employment. Some of the largest and highest paying occupations are in healthcare. This Spotlight examines employment and wages for healthcare occupations.
Fifty years of looking at changes in peoples lives
Longitudinal surveys help us understand long-term changes, such as how events that happened when a person was in high school affect labor market success as an adult.

subset: commoncrawl


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On Wednesday, June 26, the Pink Heals Tour will arrive in Murfreesboro as part of the nationwide initiative to show love and support for women battling cancer.
The tour will hold various activities, which are free and open to the public, from 10 a.m. until 2 p.m. at Middle Tennessee Medical Center.
Attractions will include the Pink Fire Trucks, a mobile mammography coach who is a part of the Our Mission In Motion bus from the Saint Thomas Health system, as well as the MTMC Mobile Health Unit.
Rutherford County first responders, firefighters and emergency medical personnel will also be in attendance.
For more information about the event, visit www.pinkfiretrucks.org.
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Expired lift!
Sorry, your requested lift from Austin to Des Moines expired on Saturday, 23 Mar 13.
Post your lift now to find a driver or passenger for your road trip, or check out current lifts from Austin to San Diego (1).
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(based on 2 reviews)
Ratings Distribution
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(3 of 3 customers found this review helpful)
Pen writes beautifully with the cartridge that it is...
By brgranger
from Gillette
Comments about Faber Castell Faber-Castell Ambition Fountain Pen:
Pen writes beautifully with the cartridge that it is provided with. The nib is very smooth, not scratchy, and it flows very nicely. The weight of the pen while writing without the cap on it is very balanced.My one complaint is that when utilizing Levenger raven black ink in a levenger converter, it dries out very quickly. If not used for a couple of minutes, it will take a half a centimeter to get it to flow again, and after leaving it overnight, I have to clean out the entire nib, making it a major inconvenience to utilize on a daily basis. Note that it does not matter how it is stored, it does it no matter if the cap is up, down, or sideways. This was not an issue with the cartridge that was provided.
Bottom Line Yes, I would recommend this to a friend
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(2 of 3 customers found this review helpful)
Good Place to Start
By KellyAnne
from Cary, NC
This is my first fountain pen and I wasn't sure what I wanted to start out with. This pen has a nice balanced weight and a slim design. I wanted the wood barrel, but it was significantly higher in price. The resin has a nice texture and it's easy to hold. If the cartridges are easy and clean to replace I think it will be a really good pen to start out with.
© Copyright Levenger Company
2016. All Rights Reserved.


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We've been keeping up on the triumphant return of Coffin Joe to film since the project was first announced, so I'm happy that we can let you guys know that production on the movie, The Embodiment of Evil, has officially wrapped!
Twitch Film got the word via an interview published in Brazil's Folha de Sao Paulo newspaper. As we've told you about before, this film has every intention of pushing the boundaries of both the audience and, if the report is to be believed, the cast and crew of the film as well; "People saw tortures. The following day, more tortures. Night would fall, and if there weren't tortures, there would be scenes with spiders, rats. The scene with the cockroaches was the most incredible."
Age has not soften the man, that's for sure. Embodiment of Evil was co-written and produced by Dennison Ramalho, director of the fantastic short film "Love From Mother Only", which you can find on Synapse Film's release of Small Gauge Trauma. Check out the official site for all things Coffin Joe to learn more about the man and his numerous films.
In further good news, Jose Mojica Marins (Coffin Joe) promised that Embodiment would not be his final film, so amazed was he by how far filmmaking techniques have come in the 20 years it's been since he made a movie. Can't wait to see what his sick mind comes up with next!
Calculate how much evil it takes to run our forums!


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Helenius vs Dimitrenko Purse Bid Deadline on Oct. 31
By Per Ake Persson
Frenchman Jean Marc Mormeck has officially left his position as the mandatory challenger for EBU heavyweight champ Alexander Dimitrenko (32-1, 21KOs). Mormeck faces unified champion Wladimir Klitschko on December 10.
Now "Nordic Nightmare" Robert Helenius (16-0, 11KOs) is in the position as the mandatory challenger for Dimitrenko. EBU have set October 31 as the deadline for purse offers.
A Dimitrenko vs Helenius fight is effectively also a world title eliminator and an exciting matchup. It´s rumored, but not confirmed, that Dimitrenko already has an offer to fight Helenius in Helsinki on December 3.
Dimitrenko will never fight Helenius, Chambers took his soul and wiped the ring with it.Comment by UglyPug on 10-17-2011
Dimitrenko fainted! lol straight fell the phuck out upon hearing the news! lol i still remember when hearing about dimitrenko fainting in the locker room. . . i know it had nothing to do with him being afraid, but you…Comment by UglyPug on 10-17-2011
[QUOTE=paulf;11305676]Comments Thread For: Dimitrenko Withdraws From Helenius Clash, Vacates Title.[/QUOTE] haha - 70/30 says it happens (dimitrenko withdraws)Comment by paulf on 10-17-2011
Comments Thread For: Dimitrenko Withdraws From Helenius Clash, Vacates Title.Comment by joe strong on 10-17-2011
a final eliminator & the winner is the EU champion & holds a WBO regional title(helenius owns it)too.the winner will get one of the klitschkos in 2012...Post a Comment - View More User Comments (11)


text: Our Verdict Attractive DAC with a decent range of connections, at a reasonable price For Rich, detailed midrange impressively sophisticated sound Against Not for fans of pumping beats and basslines Reviewed on 1st October 2008 This little DAC from Musical Fidelity might look a tad 'functional', but it's fairly well built and has a decent range of connections. There are inputs for coaxial and optical digital sources, and a square Type B USB input – so make sure you have the correct cable. In performance, the V-DAC proves itself, on the whole, to be true to its maker's sonic style: despite the reasonable price tag it offers a rich, detailed midrange, with lots of instrumental and vocal colour. If anything, this richness could get a bit much at times – especially as it means a degree of compromise when it comes to rhythmic precision. Fans of pumping beats and propulsive basslines will find something better for the money elsewhere. That said, the V-DAC is an attractive product for a very reasonable price, and renders an impressively dense and sophisticated musical picture for such a cheap upgrade. More after the break It only loses a fifth star because there are better all-rounders in its price class. Follow whathifi.com on Twitter Join whathifi.com on Facebook Related News The What Hi-Fi? Readers' Award 2016! Fri, 09 Sep 2016, 06:19pm Sony's new NW-WM1Z is a €3300 gold-plated Walkman Thu, 01 Sep 2016, 12:50pm RHA to launch two pairs of in-ear headphones and its first headphone amp at IFA 2016 Thu, 25 Aug 2016, 04:22pm Prism Sound introduces the Callia DAC and headphone amp Thu, 28 Jul 2016, 05:45pm Cyrus raises £50,000 in one day to fund SoundKey DAC Thu, 14 Jul 2016, 03:40pm DACs Hi-Fi Musical Fidelity V-DAC


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Andrew Moore reveals the tragic beauty of Detroit's ruins. Thirty monumentally-scaled photographs depict the windowless grand hotels, barren factories, collapsing churches, and blocks reclaimed by prairie grass.
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WETA looks back at the people and events of the 1980s — the disorderly decade that saw Washington's emergence as a true global capital.
Test your classical music IQ by clicking the play button to listen to the sound clips. Identify the composer for each piece and reveal your score!
Historian Garrett Peck discusses the history of brewing in the nation's capital. D.C.'s beer heritage dates back to 1770 and claims some very innovative brewers.
Nature Cat follows Fred, a house cat who dreams of exploring the great outdoors and learn about nature with the help of his animal friends.
Looking for something to do around town? Want to get the word out about your group's event? Check out our calendar!
WETA Magazine features TV schedules and highlights as well as updates about Classical WETA 90.9 FM programs.


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New DVD
An Idiot Abroad - DVD Comedy
- Summary
- Reviews / Ratings
- Photos
- Videos
- Events
In this hilarious series Ricky Gervais and Stephen Merchant, co-creators of The Office and Extras, send their friend Karl Pilkington (The Ricky Gervais Show) around the world...against his will! Karl thinks he's visiting the "Seven Wonders" but along the way the "Little Englander" must endure camel rides, jungle treks, tribal customs and local oddballs while dining on toads and testicles... and searching for a clean bathroom. How does a man who prefers vacationing via camper cope with being an idiot abroad?
Jul 10, 2015
Jul 09, 2015
Jun 24, 2014
Jun 24, 2014
More Details
Released - Tuesday, January 10 , 2012
- Starring - Karl Pilkington, Ricky Gervais, Stephen Merchant
- Distributed by - BBC
- MPAA Rating - NR


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Henson puppets 'Stuffed & Unstrung' at Pabst Theater April 13
Ever wonder what Muppets would say if someone else was pulling the strings? About the closest thing to it is "Henson Alternative: Stuffed & Unstrung," a live, adults-only improv comedy show featuring Jim Henson Company puppeteers. It'll be at the Pabst Theater at 8 p.m. April 13.
This definitely ain't "Sesame Street," and these aren't the Muppets. It's six Henson puppeteers - out in the open - with 80 puppets, working up bits and sketches onstage. Their efforts are also shown on a big screen behind them. This New York Post report gives you a taste of the experience. »Read Full Article
MSO, Swingle Singers enchant with rare 'Sinfonia'
One of the great joys of having a high-quality symphony orchestra in town is the opportunity to experience an enormous variety of music - live.
Friday's Milwaukee Symphony Orchestra concert presented a rare opportunity to hear Luciano Berio's "Sinfonia" for eight voices and orchestra. »Read Full Article(2)


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Best route map between Dover, NH and Biddeford, ME. This route take about 33 Miles.
The distance and estimated time is placed on the right depending on the status of the road.
, 23:29
* You can see Dover, NH and Biddeford, ME photos above. We provide this photos from Google Panoramio service.
* Dover, NH weather as celsius: °C
* Biddeford, ME weather as celsius: °C
* Weather information on route, provide by Open Weather Map.
All Rights Reserved. © 2010 - 2016 About Feedback


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What We Offer
The School of Education (SOE) offers baccalaureate degrees in Elementary and Secondary Education, master's degrees in Elementary and Secondary Education, Literacy, Special Education, and Humanistic Education, as well as Certificates of Advanced Study in Educational Leadership.
SOE is accredited by the National Council for the Accreditation of Teacher Education (NCATE). Most programs lead to our recommendation for New York State certification. As a graduate, you will be well grounded in content, theoretical and historical perspectives, pedagogy, the Common Core Learning Standards and clinical practice. See our students' passing rates on the New York State Teacher Certification Examinations.
Latest News
Recent Headlines
Professor Sue Books of the Department of Secondary Education was selected to welcome incoming students on behalf of the faculty at this year's convocation. She spoke candidly to the gathered students about the value of the prospects that lie before them:
"You should be proud of the efforts and achievements that have led you to this educational opportunity," Books said. "Your college years should provide you with the time, space and intellectual support to think about who you are as a member of the broader society, and about the contributions you can and want to make to it."
Professor Books, one of the School of Education's Fulbright Scholars, earned the 2014 Chancellor's Award for Excellence in Teaching. She has also received an award for lifetime achievement from her alma mater, the University of North Carolina at Greensboro. Her teaching and research interests include a focus on learning in the context of education and poverty, as well as in comparison of US and international education.


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Digital Art Trend Report
Related: Digital Experience, Digital Community, Art, Digital Strategy, Digital, Sculpture, Digital Appliance, Street Art, Digital Communication, Digital Content, Custom Reports
Get a Custom Version of the Digital Art Trend ReportThe Digital Art Trend Report features various developments made in the realm of modern art, from enhanced graphic art images and pop art photos to exhibitions solely focused on digital art galleries. In a world consumed with digital media and digital printing, it is crucial that businesses are aware of varying art forms like deviant art, 8-bit art and clip art, all forms capturing consumer attention.
Who Custom Digital Art Market Research is ForOur research is aimed at artists, photographers, programmers and web designers looking to kick start innovation. Also, our report will benefit those involved with marketing, branding and advertising, because it will reveal consumer preferences and enable you to properly position products in the marketplace. The Digital Art Report sifts through the crowded industry of modern and electronic forms of art, giving you unrivaled insight into the mind of the consumer.
Get Fast, Cost-Effective Custom Digital Art ResearchIn as little as a day, a new Digital Art Trend Report can be customized to your needs, which could include: 8 bit art, deviant art, pop art, pixelated art, digital prints and digital art trends.
Market Research MethodologyYour dedicated researcher begins by consulting with you on the topic goals, desired depth, breadth and style of report or presentation you require. Next, we start researching using the world's #1 trend platform. With specialized tools, over 300,000 researched ideas and insight from 100,000,000 people, we're able to extract 95% of the steps and costs associated with custom research. Your 50 - 100 page PowerPoint report can be created in as little as a day if your request is urgent, although a typical turnaround would be within the week.
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Other Sample Topics: Kids Decor, Drawing, Storefront, Household Routine, Pillow, Graffiti Art, Cooking Appliance, Gift Giving, Shelf, Interior Design, Open-Concept, Co-Working Space, Themed Decor, Oven, Minimalist Decor, Urban Architecture, High-Tech Appliance, Holiday Gifting, Culinary Preparation, Sink, [More]
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Sample Digital Art Market Research ReportOur Digital Art research includes hundreds of examples and Crowdsourced Consumer Insight®, hyperlinked to additional content on Trend Hunter, the world's #1 largest, most popular trend platform. Example slides:
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Mozilla announces a partnership with The New York Times, The Washington Post and Knight Foundation for a new online community platform.
Mozilla is proud to announce a new partnership with The New York Times and The Washington Post, funded by $3.89 million from the John S. and James L. Knight Foundation, to build a new, open-source content and commenting platform. The … Continue reading
Nearly three years ago, Mozilla partnered with the John S. and James L. Knight Foundation to pursue a brilliant idea: put web innovators into news organizations, and see what happens. Today we're proud to announce that the Knight-Mozilla OpenNews program … Continue reading
Today we're proud to announce the start of the 2014 Knight-Mozilla OpenNews Fellow application process. Knight-Mozilla OpenNews is looking for five developers and technologists to spend a year writing code in collaboration with reporters, designers, and developers in some of … Continue reading
Copyright © 2016 FreeSoftNews - All Rights Reserved


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Credits & Info
- Uploaded
- Jul 20, 2011 | 3:51 PM EDT
- File Info
- Song
- 3 min 14 sec
- Score
- 3.93 / 5.00
Related Content
Licensing Terms
You may not use this work for commercial purposes without making specific arrangements with the artist.
- Score:
- Rated 3.93 / 5 stars
- Plays & Downloads:
- 1,841 Plays | 171 Downloads
- Share Links:
- Genres:
- Electronic - Dubstep
- None
Author Comments
Rated 4 / 5 stars
Too many genres. That's pretty much it. Good drums, good...other samples...:|
If you're going to go down the road of dubstep, the drop at 1:19 was magnificent. lol :D
Nah you're just in time :P.
It's another "transtep" in a way. Kind of like black hole. But I think this is going to be the last time I use dubstep as a lead.
Rated 4 / 5 stars
Id take out some of the bass in the intro. The structure of the outro felt odd.
Intro has no bass.
Rated 5 / 5 stars
I like how it is right now!
Thanks :P
Rated 4.5 / 5 stars
I really liked it, but it kind of seemed like 4 different themes mashed into one.
But it was epicly put together.
Rated 4.5 / 5 stars
fast review go!
first at :47 after that climb i think there should be a big hit or crash
second at the end of the first dub break at 1:04 where it says "die" and then what sounds like cps the cps part should have a sudden steep climb and then is you can hold out the s in cps
third at 1:37 make its volume climb slowly but enough to really feel it get loud then at 1:54 keep that drop but at 1:56 make that hit, hit even harder but still back off a little for that synth
fourth at 2:25 do the same thing with the cps sound as i stated earlier and add a hit to the entrance of the end of the song but dont back off a whole lot and let the volume climb but barely it should be like you dont really hear when you casually listen to it but its there when you look for it
i hope this helped im best with dynamics and stuff so its what i tried to focus on
the rest of the song as a whole is really good
whats my time >:)
Ok, I'll work on all of that. Thanks a ton for the quick review!
Also, he's saying "Die Sapian".


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Meet Dr. Anuj Parikh
Dr. Anuj Parikh joined the Weight Loss Institute of Arizona in 2015. He attended medical school at Philadelphia College of Osteopathic Medicine and went on to complete his surgical training at Pinnacle Health in Harrisburg, PA.
During his residency, he received extensive training in minimally invasive surgery and worked closely with their bariatric surgery department, one of the busiest in the state of Pennsylvania. Throughout his training, he participated in cutting edge surgical techniques and helped conduct several research studies related to bariatric surgery. Dr. Parikh was the recipient of the Eric Hitchcock Award for Academic Excellence twice during his training, which is awarded to the resident with the highest exam scores in the program.
Dr. Parikh believes that weight-loss surgery can be an integral part of the long journey for patients to regain control of their lives. Losing weight is a difficult task and he enjoys being part of a team that provides necessary support and guidance to achieve your goals.
Dr. Parikh also participated in two surgical mission trips to India, helping those who do not have easy access to healthcare
Let our doctors specializing in weight loss for Tucson, Phoenix, Scottsdale, Mesa, Arrowhead and Tempe help you on your way to a fuller, healthier life. Request your consultation online or RSVP to one of our weight loss seminars today.
Calculate Your BMI
Body mass index (BMI) is a measure of body fat based on height and weight that applies to adult men and women


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14-3-3ơ is an intracellular, phosphoserine binding protein and proposed to be involved in tumorigenesis. However, the expression dynamics of 14-3-3ơ and its clinicopathological/prognostic significance in human tumors are still controversial.
The method of immunohistochemistry (IHC) and Western blot were utilized to examine the protein expression of 14-3-3ơ in gastric cancer and paired normal adjacent gastric mucosal tissues. Receive operating characteristic (ROC) curve analysis was employed to determine a cutoff score for 14-3-3ơ expression in a training set (n = 66). For validation, the ROC-derived cutoff score was subjected to analysis of the association of 14-3-3ơ expression with patient outcome and clinical characteristics in a testing set (n = 86) and overall patients (n = 152).
The expression frequency and expression levels of 14-3-3ơ were significantly higher in gastric cancer than in normal gastric mucosal tissues. Correlation analysis demonstrated that high expression of 14-3-3ơ in gastric cancer was significantly correlated with clinical stage and tumor invasion. Furthermore, in the testing set and overall patients, Kaplan-Meier analysis showed that elevated 14-3-3ơ expression predicted poorer overall survival (OS) and progression-free survival (PFS). Importantly, high 14-3-3ơ expression was also associated with shortened survival time in stage III and stage IV gastric cancer patients. Multivariate analyses revealed that 14-3-3ơ expression was an independent prognostic parameter in gastric cancer.
These findings provide evidence that high expression of 14-3-3ơ may be important in the tumor progression and servers as an independent molecular marker for poor prognosis of gastric cancer. Thus, overexpression of 14-3-3ơ identifies patients at high risk and is a novel therapeutic molecular target for this tumor.
Gastric cancer is one of the most common causes of cancer-related death worldwide [1], especially in East Asian countries such as China, Japan [2]. In 2005, there were approximately 0.4 million new cases and 0.3 million deaths from gastric cancer in China [3]. Despite recent advances in surgical techniques and medical treatment, the overall 5-year survival rate of gastric cancer in China remains low at about 40%. Many studies have demonstrated that multiple genetic alterations, including tumor suppressor genes, oncogenes, cell adhesion molecules, cell-cycle regulators, and growth factors, are responsible for the development and progression of gastric cancer [4]. Studies have unraveled many aberrantly expressed genes in gastric cancer including BMI1 [5], COX-2 [6], HER3 [7], RKIP and STAT3 [8], SPARC [9] and HER2 [10,11], which make risk assessment of gastric cancer patients more accurately. However, promising molecules that have clinicopathological/prognostic significance in gastric cancer remain substantially limited. It is necessary to further understand the molecular mechanisms involved in gastric cancer and to identify more valuable prognostic markers so as to not only improve poorer prognosis but also provide novel promising therapy targets.
14-3-3 proteins are a family of about 30 kD dimeric highly-conserved proteins and ubiquitously expressed in all eukaryotic organisms [12]. There are seven closely related genes (β, γ, ε, η, ζ, ơ,τ) in 14-3-3 family proteins. These genes often form heterodimers or homodimers and bind to over 100 different protein ligands [13,14]. Thus, 14-3-3 proteins are involved in many different cellular signaling processes including cell proliferation, cell cycle control, apoptosis and malignant transformation [14,15]. Among the seven isotypes, 14-3-3ơ was originally characterized as a human mammary epithelium marker 1 (HME1) and identified as a tumor suppress gene [12,16]. Growing evidences showed that 14-3-3ơ was significantly decreased or lost due to silencing of the gene via hypermethylation in several solid tumors [17-22]. Previous reports found that decreased expression of 14-3-3ơ predicted a poor survival in breast and nasopharyngeal cancer [23,24]. However, contradictory with the tumor suppressor role of 14-3-3σ, overexpression of 14-3-3ơ in pancreatic cancer cell led to drug resistance, cell migration and invasion [25]. Furthermore, high expression of 14-3-3σ was an independent prognostic factor for poor survival in pancreatic and colorectal cancer [26,27]. Thus, the biological role of 14-3-3ơ in tumorigenesis and progression of various types of human cancers varies depending on specific tumor type. Here, we selected the gastric cancer specimens with strict protocol to detect 14-3-3ơ expression dynamics and analyze their clinicopathological/prognostic significances.
A total of 216 primary gastric cancer patients from the archives of the Department of Pathology in the Third and Second Affiliated Hospital of Sun Yat-Sen University (Guangzhou, China) were initially recruited in our study. All patients underwent initial surgical resection from March 2001 to January 2006. We further screened patients using a strict eligibility criteria protocol as follows: microscopically confirmed adenocarcinoma of the stomach; without any metastatic diseases; no prior chemotherapy or radiation therapy history; receiving unified regimen as first-line chemotherapy after resection of primary tumors (we unified as FOLFOX regimen: fluorouracil, leucovorin and oxaliplatin), and no radiation treatment being administered to any of the patients; having over 5-year follow up period. Ultimately, 52 patients with loss of follow-up and 12 patients with deficiency in clinical characteristics were excluded from this study, leading to 152 gastric cancer patients subjecting to further clinical and survival analysis. In details, the overall cohort consisted of 105 male and 47 female with the median age of 58.0 year (range, 27 - 81 year). 103 patients were censused as death during the 5 years of follow-up time, including 5 cases died from postoperative complications and 98 cases died from tumor progression. Of the overall patients, 66 patients were randomly assigned by computer (SPSS 17.0 software) to the training set, and remaining 86 patients were randomly assigned to the testing set. Clinicopathological variables of the two cohorts, such as age, gender, clinical stage, tumor invasion, node stage, and histology differentiation, were included in this study. All tumors were classified and staged according to the revised guidelines advocated by the International Union against Cancer. We obtained prior patients' consent and approval from the Institute Research Ethics Committee of Sun Yat-Sen University for the use of clinical materials described in the present study.
The tissue microarrays (TMAs) were constructed as a method described previously by Xie et al [28]. Briefly, the paraffin-embedded tissue blocks and the corresponding histological hematoxylin and eosin-stained slides were overlaid for TMA sampling. Interested core tissue biopsies (0.6-mm in diameter) were punched from representative tumor areas and from adjacent gastric mucosa tissue from blocks of individual donor tissue using a trephine (triplicate cylinders from carcinoma tissue and one cylinder from normal adjacent gastric mucosa tissue). The tissue cylinders were then transferred into a recipient paraffin block at defined positions by using a tissue-arraying instrument (Beecher Instruments, Silver Spring, MD, USA).
The TMAs slides were deparaffinized in xylene, rehydrated through graded alcohol, immersed in 3% hydrogen peroxide for 10 min to block endogenous peroxidase activity, and antigen retrieved by pressure cooking for 3 min in Tris/EDTA (pH = 8.0). Then the slides were incubated with the primary antibody of 14-3-3σ (monoclonal mouse; 1:50; Santa Cruz, SC-100638) for 1 hour at room temperature. After being incubated with the secondary antibody for 30 min, specimens were stained with DAB (3, 3-diaminobenzidine). Finally, the sections were counterstained with hematoxylin, dehydrated and mounted. A negative control was obtained by replacing the primary antibody with a normal murine IgG. Known immunostaining-positive colorectal carcinoma slides were used as positive controls as previously reported [27].
The brown granules in cytoplasm of 14-3-3σ were considered as positive staining. We scored the staining intensity as follows: 0, no staining; 1+, mild staining; 2+, moderate staining; 3+, intense staining. The area of staining was evaluated as follows: 0, no staining of cells in any microscopic fields; 1+, < 30% of tissue stained positive; 2+, between 30% and 60% stained positive; 3+, > 60% stained positive. 14-3-3σ expression was evaluated by combined assessing of staining intensity and extension. The minimum score when summed (intensity + extension) was 0, and the maximum was 6. The criteria used in this study has been widely accepted previously [24]. Expression of 14-3-3σ was assessed and scored by two independent pathologists (Drs. F Tang and ZY Feng) who were blinded to the clinicopathological data. The agreement of these two pathologists on the IHC score reached to 84% (128 identical scores in total 152 cases), suggesting our scoring system was highly solid and reproducible. If the results reported by the two pathologists were consistent, the value was selected. However, the interobserver disagreements (approximately 6% of total cases) were reviewed for a second time, followed by a conclusive judgment by both pathologists.
The gastric cancer and paired normal adjacent gastric mucosal tissues were ground and lysed with the RIPA buffer on ice before being subjected to Western blot analysis. The protein concentration was detected by the Bradford method with BSA (Sigma-Aldrich) as the standard. Equal amounts of cell and tissue extract (40 μg) were subjected to SDS-PAGE and transferred to nitrocellulose membrane (Bio-Rad) for antibody blotting. The membrane was then blocked and incubated with mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody (Abmart, #M20006) and mouse anti-14-3-3σ antibody (Santa Cruz, SC-100638).
The ROC curve analysis was subjected to the selection of 14-3-3σ cutoff score in the training set, as described previously [29]. Briefly, the sensitivity and specificity for the outcome being studied at each score was plotted to generate a ROC curve. The score localized closest to the point at both maximum sensitivity and specificity, the point (0.0, 1.0) on the curve, was selected as the cutoff score leading to the greatest number of tumors which were correctly classified as having or not having the outcome. To facilitate ROC curve analysis, the survival features were dichotomized: survival (death VS. others (censored, alive or death from other causes)).
All patients had follow-up records for over 5 years. After the completion of therapy, patients were observed at 3 month intervals during the first 3 years and at 6 month intervals thereafter. Overall survival was defined as the time from diagnosis to the date of death or when censused at the latest date if patients were still alive. Progression-free survival was defined as the time from diagnosis to the date of local failure/distant metastasis or the date of death or when censused at the latest date.
For survival analysis, optimal cutpoint for 14-3-3σ expression was obtained by ROC analysis in the training set (n = 66). For validation, the relationships between 14-3-3σ expression, which was classified by ROC analysis-generated cutoff point, and OS, PFS were evaluated in the testing set (n = 86) and overall patients (n = 152). The chi-square test or Fisher's exact test was employed to evaluate the relationship between 14-3-3σ expression and clinicopathological variables. The multivariate Cox proportional hazards model was utilized to estimate the hazard ratios and 95% confidence intervals for patient outcome. The relationships between 14-3-3σ expression and OS, PFS were determined by Kaplan-Meier analysis. The log-rank tests were performed to value the difference in survival probabilities between patient subsets. All p values quoted were two-sided and p < 0.05 was considered statistically significant. Statistical analysis was performed using SPSS v. 17.0 (SPSS, Inc, Chicago, IL).
14-3-3σ was detected in the cytoplasm and overexpressed in the gastric cancer (Figure (Figure1A1A and and1A'),1A'), whereas normal paired gastric mucosal tissues showed nearly negative expression (Figure (Figure1B1B and and1B').1B'). Consistent with this result, Western blot analysis revealed a similar finding in gastric cancer and normal adjacent gastric mucosal tissues (Figure (Figure1C1C).
To further assess survival analysis and avoid the problems of multiple cutpoint selection, ROC curve analysis was employed to determine cutoff score for 14-3-3σ expression. As shown in Figure Figure2A2A and and2B,2B, the 14-3-3σ cutoff score for OS and PFS in the training set was 3.25 (p < 0.001) and 3.35 (p = 0.003) respectively. We thus selected a 14-3-3σ expression score of 3 (> 3 VS. ≤ 3) as the uniform cutoff point for survival analysis in the testing set.
The clinical features of these two cohorts of patients, including age, gender, clinical stage, tumor invasion, node stage, histology differentiation, 14-3-3σ expression, were summarized in Table Table1.1. ROC-derived 14-3-3σ cutoff score of 3 in the training set successfully segregated the testing set into high (44/86, 51.2%) and low (42/86, 48.8%) 14-3-3σ expression subgroups. High expression of 14-3-3σ was mainly found in more advanced tumor stages (77/107 in stage III+IV VS. 12/45 in stage I+II, p = 0.004). Furthermore, correlation analysis demonstrated that high 14-3-3σ expression was correlated with clinical stage (p < 0.001 for both set) and tumor invasion (p < 0.001 for training set and p = 0.005 for testing set) in both sets. 14-3-3σ associated to patients' age in the testing set (p = 0.01) but not in the training set. We failed to detect any relationship between 14-3-3σ with other patient characteristics, including gender, node stage, and histology differentiation.
As shown in Figure Figure3A3A and and3C,3C, Kaplan-Meier analysis showed that elevated 14-3-3σ expression strongly predicted an inferior OS in the testing set (p < 0.001) and overall patients (p < 0.001). Moreover, 14-3-3σ expression was also a powerful prognostic factor for PFS in the testing set (p < 0.001, Figure Figure3B)3B) and overall patients (p < 0.001, Figure Figure3D).3D). Considering the poor outcome in late-stage gastric cancer, further analysis was performed with regard to 14-3-3σ expression in subsets of gastric cancer patients with stage III and IV. The results demonstrated that compared with 14-3-3σ low expression, high expression of 14-3-3σ showed a significant trend toward worse OS and PFS in gastric cancer patients of stage III (p = 0.001 for OS and p = 0.017 for PFS, Figure Figure4A4A and and4B)4B) and stage IV (p < 0.001 for OS and p = 0.001 for PFS, Figure Figure4C4C and and4D).4D). Results in the overall patients were similar to those found in the testing set (Figure 4E-H).
To avoid the influence caused by univariate analysis, the expression of 14-3-3σ as well as other parameters was examined in multivariate Cox analysis (Table (Table22 and Table Table3).3). In the testing set, 14-3-3σ was indeed found to be a significant independent prognostic factor for poor OS (hazard ratio, 4.527; 95% CI, 2.274-9.012; p < 0.001; Table Table2)2) and PFS (hazard ratio, 3.582; 95% CI, 1.717-7.475; p = 0.001; Table Table2).2). Similar results were also observed in overall patients (hazard ratio, 5.161; 95% CI, 2.990-8.909; p < 0.001 for OS and hazard ratio, 4.416; 95% CI, 2.482-7.856; p < 0.001 for PFS; Table Table3).3). Of other parameters, clinical stage was found to be an independent prognostic factor for patient survival in the testing set but not in overall patients.
Gastric cancer is one of the most common cancers worldwide and it poses one of the most serious public health problem [1]. Recurrence and metastasis are still the major issues for the poor survival of advanced gastric cancer patients [30]. Although previous studies have found that many aberrant expressed genes in gastric tumor can help classify the risk of patient outcome [5-11], more novel molecular markers that can identify tumor progression and predict the prognosis individually are still urgently needed. The 14-3-3 family proteins have gained much attention over the past years due to their involvement in cancers by regulation of diverse cellular processes [14,15]. Among the seven isoforms, 14-3-3σ is up-regulated by p53 in response to DNA damage, and sequesters the essential mitotic initiation complex, cdc2-cyclin B1, from entering the nucleus, thus preventing the initiation of mitosis [31]. As a result, 14-3-3σ induces G2 arrest and allows DNA damage repair. Thus, different from other members of the family, 14-3-3σ is defined as a negative regulator of cell cycle checkpoints and a potential tumor-suppressor protein [12]. However, the biological role of 14-3-3ơ in tumorigenesis and progression of various types of human tumors remains controversial. Here, to further reveal the biological function of 14-3-3ơ in gastric cancer, we studied 14-3-3ơ expression dynamics and analyzed their clinicopathological/prognostic significances in 152 tumor specimens.
In this study, to develop an objective 14-3-3σ cutoff point for survival analysis, we used the ROC curve analysis to generate a cutoff score in the training set. 14-3-3σ expression, which was classified as high and low level by the ROC-derived cutoff point, was mainly found to be higher in more advanced tumor stages (stage III and IV), indicating that 14-3-3σ might be involved in gastric cancer progression. Correlation analysis further demonstrated that high 14-3-3σ expression was associated with clinical stage and tumor invasion in gastric cancer (Table (Table1).1). Furthermore, in the testing set and overall patients, high 14-3-3ơ expression predicted a significant OS and PFS disadvantage over low 14-3-3ơ expression subgroup (Figure (Figure3).3). Importantly, worse prognostic impact of increased 14-3-3σ expression was demonstrated in patients with stage III and IV tumors (Figure (Figure4),4), indicating that 14-3-3ơ might be a novel factor for risk definition in gastric cancer. In addition, multivariate analyses in the testing set and overall patients revealed that 14-3-3ơ expression was an independent prognostic parameter. Taken together, our findings in this study provided evidence that elevated expression of 14-3-3σ in gastric cancer might facilitate an increased malignant and worse prognostic phenotype of this tumor.
With regard to the prognostic impact of 14-3-3σ in different human cancers, some of the reported data are totally contradictory. It was documented that loss expression of 14-3-3σ was linked to a poor prognosis of patients with breast and nasopharyngeal cancers [23,24]. However, consistent with the results found in pancreatic and colorectal carcinomas [26,27], our finding in this study showed that high expression of 14-3-3σ was positively associated with clinic stage and poor survival. The underlying mechanism(s) of 14-3-3σ to impact cancer prognosis might be depended on intrinsic properties of the tumor type. Moreover, different co-expression of 14-3-3σ and other molecules [24], as well as the nature of the therapeutic regimen in various types of human cancers [32], which further make us to understand that the function of 14-3-3σ and its underlying mechanism(s) to impact cancer prognosis may be tumor-type specific. Recently, studies have showed that overexpression of 14-3-3σ is correlated with tumor metastasis and progression in pancreatic [25], breast [33] and ovarian cancer cells [34], which may further explain our findings that higher 14-3-3σ expression is mainly detected in more advanced tumor stages and also identified as a poor prognosis indicator in late-stage gastric cancer patients. Hence, our study demonstrated that 14-3-3σ was an independent prognostic biomarker for OS and PFS in gastric cancer. Especially, overexpression of 14-3-3σ was relevant with tumor progression. The findings reported here could have clinical value in predicting the prognosis of gastric cancer and identifying gastric cancer patients that are at high risk of progression and recurrence.
In summary, in this study, we describe 14-3-3σ expression in gastric cancer and normal gastric mucosal tissues. Our results provide a basis for the concept that increased expression of 14-3-3σ in human gastric cancer may be important in the tumor progression and serves as an independent biomarker for poor survival. Thus, overexpression of 14-3-3ơ identifies patients at high risk and is a novel therapeutic molecular target for gastric cancer.
The authors declare that they have no competing interests.
WHZ designed the study, evaluated the clinical records and drafted the manuscript. FT carried out the immunohistochemistry assays and performed the immunohistochemical analyses. JX participated in the statistical analysis, carried out the Western blot assay and performed the immunohistochemical analyses. XW helped to draft the manuscript. ZYF performed the immunohistochemical analyses. HGL and DJL analyzed the data. CKS and QL participated in the design of the study, in its analysis and in the interpretation of the data. QL also participated in writing the manuscript. All authors read and approved the final manuscript.
The pre-publication history for this paper can be accessed here:
The work was supported by grants No. 30772476, 30873084 (Q Liu), 30672409 and 81071893 (CK Shao) from the National Natural Science Foundation of China.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


text: Industrial Estates Industrial Estates Industrial Estates The Estates and Valuation section within the Environment Directorate manages the industrial starter units that Neath Port Talbot County Borough Council has available. There are ten industrial estates (see menu on the left) with approximately 220 units throughout the county borough available to starter businesses. Units/offices range in size from 13.94 sq metres (150ft) to 464.52 sq metres (5,000ft) which cater for a wide range of commercial uses, however under planning regulations no retail is permitted. View estates on interactive map Current availability of industrial units Vacancies Estates Main Current Vacant Units Area OFFICE D LONLAS VILLAGE WORKSHOPS 140FTsq 13Msq OFFICE E LONLAS VILLAGE WORKSHOPS 151FTsq 14Msq OFFICE F LONLAS VILLAGE WORKSHOPS 151FTsq 14Msq OFFICE K LONLAS VILLAGE WORKSHOPS 312FTsq 29Msq OFFICE M LONLAS VILLAGE WORKSHOPS 118FTsq 11Msq UNIT 30 LONLAS VILLAGE WORKSHOPS 3940FTsq 366Msq OFFICE N LONLAS VILLAGE WORKSHOPS 322FTsq 30Msq Frequently Asked Questions Who Can Apply? How can I view a property? How much are the business rates? Is there Business Advice available? Will my rent go up during the lease? What happens if I don't pay my rent on time? Is there a 'cooling off' period? What happens when my lease comes to an end? How do I change the use? What are the Charges/Fees? What methods of payment are available? What maintenance will YOU be responsible for? What services do the premises have? Is storage allowed outside the premises? What Application Form References are required Legislation? Who to Contact? Who can apply? The industrial units are provided to assist businesses and therefore the local economy in their areas. Accordingly applications will not be considered from individuals wishing to use them for their own private use e.g. storage of household items. Back To Top How can I view a property? First of all we recommend that you visit the property to ensure that the location would be suitable for your business. Before an appointment can be made for an internal viewing of a property, new businesses need to provide a business plan of their intended venture. Established businesses need to provide a letter on intention, outlining as much information about the business, i.e. how many jobs will be created? What experience you have? How long in business? Etc. Applications or enquiries will be received by e-mail in writing or complete our Contact us form Back To Top How much are the business rates? You will be responsible for paying business rates separately to the Council. You can ascertain the Rate on the Valuation Office Agency's website or by contacting the Council's Non-Domestic Rates Section on 01639 764328 Back To Top Is there Business Advice Available? The Authority is able to provide professional business advice to prospective tenants such as finance, grants etc. please contact our Economic Development Unit on 01639 686835. Back To Top Will my rent go up during the lease? Your lease will specify when the rent will be reviewed. This usually means that the rent will increase to reflect any increases in market rents for similar properties. In some cases, the increase is a fixed sum agreed when the lease was first granted. It is important to check your lease to be sure whether there will be a rent review and, if so, how the new rent will be decided. The rent payable under a tenancy will normally be reviewed by written notice or a notice to terminate the tenancy with the offer of a new tenancy, at a new rent. The length of notice which must be given should be stated in your tenancy agreement. Back To Top What happens if I don't pay my rent on time? We expect our tenants to pay rent and service charges on time. If an account falls into arrears, it is likely that the Council will charge interest in accordance with the terms of the lease. It may be necessary for bailiffs to be instructed to collect the amount owed and payment of their costs. This procedure involves extra expense for the tenant, so it is in your interests to make payment you will save money if you pay the rent when due. If you are having problems paying on time, it is important that you inform us of the reasons as soon as possible. Then we may be able to discuss a way forward and offer a solution. Back To Top Is there a 'cooling off' period? Once the tenancy agreement has been signed you are legally bound by your specific agreement. If you wish to terminate your agreement, this document will state the required notice period. Back To Top What happens when my lease comes to an end? This is a complicated subject on which you should get advice from a Solicitor or a Chartered Surveyor experienced in commercial property management. Although the length of the lease may seem to be clear, the law says that in some circumstances a lease will continue unless the landlord or the tenant takes specific action to bring it to an end by the service of written notice in the prescribed form and for the correct period. You should not assume you can merely vacate the property at the end of the lease, as you will continue to be liable for rent if you haven't given proper written notice. How do I change the use? If you are already a tenant, the Council as your landlord and owner must agree to the change of use specified in your lease or tenancy in writing. You will have to pay the costs of this and must make an application by e-mail or letter to the Estates & Valuation Section. You may also have to make an application for planning permission to change the use and pay the appropriate fee. The Planning Department will advise you whether planning permission is required and the amount of the fee. They can be contacted by telephoning 01792 764281 The Council's tenancy or lease requires that tenants comply with the use specified in the tenancy or lease. Back To Top What are the Charges/Fees? It is wise to remember, that as a commercial tenant, you will be liable for a number of accommodation related costs which are above the normal day to day running expenses. Typically the following costs may also apply: Rent / Licence Fee You will be responsible to pay the Council rent for the premises you are occupying. All rental payments are due as stated in your lease or tenancy agreement and are normally payable monthly in advance. Payment is usually by direct debit. Insurance The Council insures the premises, the premium being recharged to the tenant. However, it is your responsibility to satisfy yourself as to the extent of cover, to obtain additional cover if required and to obtain contents insurance. Service Charges Normally apply to commercial premises. If they apply they will be detailed within your lease and could cover services such as repairs, cleaning, security etc. What methods of payment are available? Cash Cheque Direct Debit Debit Card However on completion of your tenancy agreement the Council will ONLY accept cash or a banker's draft, for the deposit the first months rent and the annual building insurance premium. What maintenance will YOU be responsible for? Again this will depend on the specific clauses in your Tenancy Agreement. However On the majority of the estates the tenant will be responsible for the internal aspect of the premises including the roller shutter door, windows and doors. Otherwise the Council will maintain the external fabrication of the premises and the surrounding area including the estate sign. What services do the premises have? It is understood that each unit has electricity and water, although interested parties should make their own enquiries as to the units suitability. Back To Top Is storage allowed outside the premises? Again this will depend on the specific clauses in your Tenancy Agreement. However External storage is strictly prohibited at each of the Estates, unless the unit has a compound. Action will be taken by the Council should a tenant fail to comply with this requirement. Back To Top What Application Form References are required? For applications to rent property you will need to supply a bank reference and two trade or character references. We will send you an application and reference form to complete and return. Some Financial institutions charge for this service however this is included in the fees you pay. Back To Top Legislation Although the Council is landlord, it is separate and independent from its role as Planning Authority. Whilst the landlord may indicate approval to a particular trade, this does not mean that you have planning permission to commence that trade. You are required to make your own enquiries as to the suitability of your business. Back To Top How to Contact Us? Please complete our Contact Form. Please allow 48hrs for reply before contacting the following Estates Officers in the Estates & Valuation Section, by contacting Richard Ford, 01639 686694 It will help us if you include in your enquiry your telephone or mobile phone number and your full postal address. If your enquiry is about a specific property it will help us if you are able to describe it by the postal address or in relation to the property next door to it. Disclaimer – Neath Port Talbot County Borough Council have made every attempt to ensure that the information provided on these web pages are correct. The particulars displayed do not constitute an offer or any part of an offer. Prospective tenants should satisfy themselves of the accuracy of the information provided. Back To Top


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Please visit us at Coiltech 2016 in Pordenone! We will be pleased to meet you!
Coiltech is an international exhibition dedicated to the Coil&Winding industry. There you will discover the last trends about materials and machineries for the production of electric motors, generators, transformers and winding systems.
Fix an appointment if you need much time with us to speak about a project or simply come there to ask us about our last developments. Single poles selection and assembling, magnets insertion, Backlack automation and new control lines are just a few of the new tendancies that Dema has recently made for its Customers.
Dema stand is Nr B12 in the Hall Nr 9 (just in front of the main entrance). For any further information, please contact us at firstname.lastname@example.com or call us!
How is changing the manufacturing process of electric motors?
Dema is developing the answers to that question concerning the ambit of the rotor and stator laminations and stack handling.
Dema is currently developing research & development projects in 3 areas:
Loose laminations handling to move them from the blanking press
Full automatic Backlack production lines to produce Backlack rotors, stators and single poles
High speed control process to check interlocked stacks directly downstream of the blanking press
Be in touch with the future….contact Dema!!!
Dema now can propose to its customers many solutions to assemble the armature.
The classic solutions are:
Both machines can use loose laminations or interlocked stacks.
Recently Dema developed some new stand-alone machines able to assemble end fibers and commutator, that can be integrated downstream the Basic Armature Machine and complete the armature production process:
By today you have the opportunity to improve your armature production process step by step, choosing which production steps you want to make more reliable.
Please contact Dema if you improve your armature production process!!!
Any smart invention can be improved: Dema presents the Electric "ShaftCut" Machine
The notching process is one of the cheapest methods to grant the interference between shaft and lamination stack and / or shaft and commutator. Dema has designed and developed a machine whose name is "E-ShaftCut" that could be stand-alone or integrated in line. This machine is a full automatic station for cutting rotor shafts by an electric press.
The main purpose is to guaranty a high repeatability of shaft / lamination stack and of shaft / commutator coupling process.
The use of the electric press increases the process stability; when the coupling changes due to the components tolerance variations the machine reacts automatically.
This big advantage has to be added to the other advantages of the basic hydraulic machine: cheaper shaft cost and reduction of the scrap armatures produced.
The final result on using the E-ShaftCut Machine is to guaranty process quality, high reliability and easy configuration and setting.
Please, ask us more about "E-ShaftCut" Machine!
Like every year, Dema is waiting for you at the CWIEME Fair in Berlin, the World's largest international coil winding, insulation and electrical manufacturing exhibition. This is a fix appointment for Dema and also this year from 10th to 12th of May we will be pleased to meet you in our stand.
Our stand is situated in the upper Hall 3.2 and the stand number is D32C39.
For any further information, please contact us at email@example.com or simply call us!
Dema is carrying on a european project of R&D. The project name is Spark, programm Horizon 2020.
Horizon 2020 has the political backing of Europe's leaders and the Members of the European Parliament. They agreed that research is an investment in our future and so put it at the heart of the EU's blueprint for smart, sustainable and inclusive growth and jobs.By coupling research and innovation, Horizon 2020 is helping to achieve this with its emphasis on excellent science, industrial leadership and tackling societal challenges. The goal is to ensure Europe produces world-class science, removes barriers to innovation and makes it easier for the public and private sectors to work together in delivering innovation. Horizon 2020 is open to everyone, with a simple structure that reduces red tape and time so participants can focus on what is really important. This approach makes sure new projects get off the ground quickly – and achieve results faster. The EU Framework Programme for Research and Innovation will be complemented by further measures to complete and further develop the European Research Area. These measures will aim at breaking down barriers to create a genuine single market for knowledge, research and innovation.
Dema is focused on development of technologies needed for the use of Backlack laminations: Backlack, T-poles (automatic production), bonding process, specific tools, T-poles winding, stacks final assembling, post processing activities and quality control. The phase 1 of the project is already achieved, so Dema is now already working on phase 2.
Dema wishes to all its Customers and Contacts a MERRY CHRISTMAS and a HAPPY NEW YEAR 2016.
Concerning the optimal exploitation of the material, the sheet metal cost imposes to minimize blanking scraps; this has led to the development of the tendency of producing the stator by assembling its small-size portions, purposely drawn from sheet metal zones otherwise eliminated as scrap. The single poles of a stator lamination are then blanked one by one, to be first assembled in stator poles, called "T Poles", and successively, assembled to form the stator in its definitive geometry.
Talking about the drastic reduction of the productive process cost, linked with the capability of automating the process itself, it is necessary to take into account that the productive process of "Backlack" stacks needs several stages: handling of single laminations, assembly of stacks , loading and unloading of the equipment used in the thermal cycle, thermal cycle, machining of the produced stacks and final packaging. Hence the choice of the opportune automation is influenced by countless factors.To realize the production cycle, loose lamination stacks must be placed inside proper equipments, to be designed case by case, which, besides determining the quality of the produced stacks , at the same time establish the quantity of used energy. Finally, the choice of the heating and cooling process depends on the choice of the automation degree of the productive process in its whole, which in its turn influences the definition of the necessary equipment.
Please visit us at Coiltech 2015 in Pordenone! We will be pleased to meet you!
Coiltech is an international exhibition dedicated to the Coil&Winding industry. There you will discover the last trends about materials and machineries for the production of electric motors, generators, transformers and winding systems.
Fix an appointment if you need much time with us to speak about a project or simply come there to ask us about our last developments. Single poles selection and assembling, magnets insertion, shaft cutting and Backlack are just a few of the new tendancies that Dema has already made real.
Dema stand is Nr B12 in the Hall Nr 9 (just in front of the main entrance). For any further information, please contact us at firstname.lastname@example.com or call us!
In the field of the production of stator and rotor stacks for electric motors, several systems are available for industry to implement a "single body" provided with the opportune stability. The most common machining processes include interference, interlocking , welding, crimping or point-to point bonding. The stacks manufactured with the "Backlack" process stand out for high rigidity and high electromagnetic efficiency.
Thanks to the use of this technology, the laminations composing stator and rotor stacks stably adhere one another thanks to a glue that acts on their entire surface. The "Backlack" sheet metal, in fact, is uniformly coated on both sides by insulating paint with adhesive characteristics. When the adhesive properties are activated, each portion of a lamination surface is steadily anchored to the one of neighbouring laminations, conferring strong compactness to the systems and at the same time maintaining a perfect electric insulation. Those features have facilitated its use, until now, in the sectors where rigidity and electromagnetic efficiency are very important, in particular in hybrid and electric cars, in energy generators and in electro-medical equipment.


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19 vehicles matched;
Now showing page 1 of 1. 25 results per page.
Listed by Private Seller
Too many projects, I need to cut some loose, this is a 1968 Plymouth GTX, it was a 440 auto,
Listed by GAA Classic Cars Auction (Auction)
Listed by GAA Classic Cars Auction (Auction)
Listed by Flemings Ultimate Garage
68 PLYMOUTH GTX 440 Beautiful example of this iconic muscle car, Chryslers Legendary 440cid V-8 @ 375hp, Upgraded MSD
Listed by Ideal Classic Cars
1968 Plymouth GTX powered by the legendary and numbers matching 440CI 375 horsepower engine and numbers matching 3-speed Torqueflight automatic
Listed by Private Seller
1968 Plymouth GTX 440 4 speed one of only 174 produced 1968 Plymouth GTX Convertible 440 4 speed and dana 60
Listed by Streetside Classics - Nashville
A 1968 Plymouth GTX that's factory-correct inside and out, and even has it original 440 big block under the hood?!
Listed by NBS Auto Showroom
1968 PLYMOUTH GTX CONVERTIBLE PRICED TO GO! We adjust our prices down daily so do not wait because someone else
Listed by Private Seller
Excellent condition 1968 Plymouth GTX for sale. This a ground up, rotisserie build. Alot of time, care and attention have
Listed by PJ's Auto World
1968 Plymouth GTX for Sale. Powered by a 440 CID topped off with an Edelbrock intake manifold carrying 3
Listed by Baltria Vintage Auto Gallery
1968 Plymouth GTX. 440 C.I. 4-speed floor shift manual trans. All Original. Dana 60 sure grip / ratio 3:54/3:55.
Listed by R & R Classic Cars
1968 Plymouth GTX Convertible
Just in from a private collection is this ultra rare low production muscle Mopar. Finished in
Listed by Private Seller
1968 GTX 2dr Hardtop Special - Original 440 1-4BBL V8 with matching Original 4 Spd. Transmission. Car is not
Listed by Volo Auto Museum
This GTX is stunning in velvet black with red accents. Has dyno tuned 440 Magnum engine, 4 speed, and a
Listed by Custom Rods & Muscle Cars
Listed by Private Seller
Classic, one-owner 1968 Plymouth GTX. Excellent condition, 2 door, 440, automatic on-floor. Original green paint color with green
Listed by Private Seller
This car is in immaculate condition.It is a show car I only take it to shows. I've included a picture
Listed by Heartland Classics
This car is almost completely restored. I would say its 98% done. We were going to finish it
Listed by Monaco Luxury
1968 Plymouth GTX Convertible: If your collection lacks an immaculate Plymouth big block convertible, look no further! And if you


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Fitzwater brings Piaf alive in Skylight's 'Onstage'
The music and life story of popular French singer Edith Piaf get under one's skin.
Singer-actress Leslie Fitzwater, who has brought Piaf to the stage several times over the course of her career, opened her one-woman "Edith Piaf Onstage" at the Skylight Music Theatre on Friday evening. »Read Full Article
10 champion — or otherwise cute — cats from the regional cat show
Over 200 cats competed this weekend in Waukesha at the Great Lakes Regional Pedigreed & Household Pet Cat Show. Here are some local and out-of-state title-bearing competitors.
1) When Teddy isn't being flopped upside-down by his owner and best friend, he's also No. 1 Ragdoll Alter and No. 13 Allbreed Alter in the world. »Read Full Article(4)
'Enter the Dragon,' 'Space Odyssey' booked for Film Festival
The Milwaukee Film Festival will screen two hall-of-fame candidates this fall as part of its Cinema Hooligante lineup of genre films.
MFF will show Bruce Lee's kung-fu fightin' classic, "Enter the Dragon" (1973) and Stanley Kubrick's sci-fi masterpiece "2001: A Space Odyssey" as part of its late-night showcase, otherwise devoted to "raunchy, gory, scary, fun, bizarre and possibly offensive midnight, cult and genre films," the festival tastefully boasts. »Read Full Article(1)
On Tap Today: The zany adventures of artist Billy X. Curmano
Cows deserve more art shows.


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In light of severe weather heading towards Southwest Georgia during the course of the day on Thursday, the National Weather Service has issued multiple watches for Southwest Georgia.
A tornado watch has been issued for Baker; Calhoun; Clay; Decatur; Dougherty; Early; Grady; Lee; Miller; Mitchell; Quitman; Randolph; Seminole; and Terrell counties until 7 PM .
Weather.gov states that a tornado watch means that conditions are conducive to the development of tornadoes in and close to the watch area.
A freeze watch has been issued for Baker; Ben Hill; Berrien; Brooks; Calhoun; Clay; Colquitt; Cook; Decatur; Dougherty; Early; Grady; Irwin; Lanier; Lee; Lowndes; Miller; Mitchell; Quitman; Randolph; Seminole; Terrell; Thomas; Tift; Turner; and Worth counties until December 22nd at 9 AM.
According to weather.gov, a freeze watch means sub-freezing temperatures are possible for at least two hours.
Stay connected to MySouthWestGA.com as stories develop and the FOX 31 Newscast at 10 PM. Visit us on Facebook and Twitter to join in on the conversation and connect with FOX 31!


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Just wondering how many of you out there are having baby #4???
Baby #4?? Anyone else???
Mommy to:
Donovan (3.31.07) 9lbs
Vala (10.31.08) 9lbs 7oz
Pagan (6.2.10) 9lbs 15oz
Beckett (1.9.12) 11lbs 7oz BORN AT HOME!
Studying to become a MIDWIFE!
this is #4 for us but we have a really big gap between the last one and this one.....11 years to be exact! the oldest will be 17 when this one arrives!
Me-36 DH-41
Cayleigh Grace 1-4-2012
I'm on baby number 6
�I eloped with my ? on 11-3-09
DS-9 DS-6 DD-5 DS-3 DS 9 Mos.
Expecting a winter baby on 1*11*2012 then we're closing up shop for good!
Just found out today that number four is on the way! I have a son and two daughters already so I'm kind of hoping for a boy but I'll be happy with a girl. I have a sneaky suspicion that it's twins though and that would give my poor DH a heart attack since our oldest is only 5!
Jesse(7), Leah(5)(.Y.)Bronze!, Lilyana(3)(.Y.)Silver!, &Jonah(14mo)(.Y.)Gold!. TTC#5!
:D Congrats :)
Mommy of 7 boys... hoping lucky number 8 is a girl!!! EDD 1/4/12
5 year old boy, 4 year old girl, 2 year old girl and bean on the way due 1/3/12. We can't wait!!!!!!! I am kind of hoping for a boy but would be more than thrilled to add to all the pink in our house!!!
Feel well everyone!
Me too Me too! Baby #4 due 1/3/2012! Probably a week early since it will be a repeat c-section since my last baby (and only girl) decided she was going to walk out feet first (shame on her!) Super excited to find other moms having a 4th! So just curious what have your reactions been to #4 if you have made the announcement yet? Mine have all been really positive, a few of the were you ttc? And only 1 you know how to prevent that right? Which I was super offended by grrrrrrr, especially since it was a fb comment from someone I dont really know that well. But everyone else seems really happy they keep saying they hope it's another girl, me I'll be thrilled either way!
Brittany Love my hubby�2002�
�( . )( . ) double platinum David 2002 (brain aneurysm survivor) Connor 2006 (premature)Brooklyn 2009, Baby Boy due 1/2012
We have only told my mom and older brothers. We are waiting for Mother's Day to tell the rest of the family and the kids. Then Monday I can finally tell all the girls at work and such:) Most everyone knew that we were ttc #4 so I don't expect many to be surprised! Even the kids know we have been 'asking God to give us a baby":)
Married 10 yrs to DH
DS March 2005 � � �DD May 2009
DS Januray 2007 � Little To Be EDD January 2012
Congrats Brooke!
I won't spill the beans if you won't! We didn't tell anyone we were trying again.
Anna-Medela Mom Maven ( . Y . ) Wife to Josh 4/14/07
Evan 5/21/08 Andrew 9/4/09 Stella 5/13/12 Liam 8/20/13 Baby #5 due Oct. 1st
Learn more
Look how far your child has come.
Review your child's growth milestones.
Read about 4 years 7 months : Week 2.
Your growing child
Your 4 1/2-year-old: Tall tales
Your 4 1/2-year-old: Beating bossiness
Your 4 1/2-year-old: Building healthy habits
Your 4 1/2-year-old: Getting into scrapes
Your 4 1/2-year-old: Teaching generosity
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What's next for your growing child?
Get a glimpse of what's to come.
Read about 4 years 9 months.