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Mashed Potato Casserole #5FIX
Total Time
Prep 45 mins
Cook 15 mins
5-Ingredient Fix Contest Entry. It is suitable for any holiday meal with meat, chicken or fish. I have been preparing it for so many years, I do not remember its origin.
Ingredients Nutrition
- 5 lbs Simply Potatoes Traditional Mashed Potatoes or 5 (24 ounce) bags Simply Potatoes Traditional Mashed Potatoes
- 3⁄4 cup butter (room temperature)
- 1 1⁄2 cups half-and-half cream (warm in small pot)
- 12 ounces swiss cheese
- 2 (3 ounce) packages cream cheese with chives
- Preheat oven to 350. ** After potatoes have been boiled, place in pot. Cut up swiss cheese in small pieces before adding to pot. Then add the rest of the ingredients & whip with a hand blender. Crock pot method: Place everything in pot and place on Lo or Hi depending on your meal time. Oven method: Place in two casserole dishes in preheated oven for 15-20 minutes.
- **Warm milk & butter should be room temperature to make easier blend.


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Rent An Introduction to Human Services 6th edition today, or search our site for other textbooks by Marianne Woodside. Every textbook comes with a 21-day "Any Reason" guarantee. Published by Brooks Cole.
Need help ASAP? We have you covered with 24/7 instant online tutoring. Connect with one of our tutors now.

subset: commoncrawl


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moths are really cool
Photos of moths seen during my early morning moth photo walk in Baguio City, Philippines
Photos I took durng my early morning moth photo walk - July 7, 2016
綠背斜紋天蛾 (黃腹斜紋天蛾) Yam Hawk Moth (Theretra nessus)
Reference :
A Photographic Guide to Hong Kong Insects 香港昆蟲圖典 1st Edition 2014 P.489 - Hong Kong Entomological Society
Insecta: Lepidoptera
Sphingidae, Macroglossinae
Theretra nessus
Tai Yeung Che, Lam Tsuen Valley, Tai Po, New Territories, Hong Kong
Yam Hawk Moth
Order: Lepidoptera
Family: Sphingidae
Genus: Theretra
Species: T. nessus
Insecta: Lepidoptera
Sphingidae, Macroglossinae
Theretra nessus
Wong Lung Hang Road, Lantau Island, Hong Kong
The Yam Hawk Moth (Theretra nessus) is a moth of the Sphingidae family. It is found in Sri Lanka, India (including the Andaman and Nicobar Islands), Nepal, Burma, Thailand, southern China, Taiwan, South Korea, Japan, Malaysia, Indonesia, northern Australia and New Caledonia. It is a recent immigrant in Oahu.


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WellingtonNew Zealand
September 9, 2012
No answer yet
Hi, I'm Tanja - just returned to New Zealand shores from Berlin. Keen to get involved, enjoy the outdoors and meet interesting people.
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All Product Details
To make visible this invisible army of compassion, Mary Fisher set out to photograph caregivers of people - children, women, men - with HIV and AIDS.
Twenty quiet heroes won the annual National Outstanding Caregiver Award from Mary Fisher's Family AIDS Network. Mary Fisher's compassionate text and photographs vividly record the people whose resolute acts of mercy and love make them God's Angels in the fight against AIDS.
In over ninety photographs Mary Fisher captures extraordinary moments of love, faith, and courage in the lives of AIDS caregivers.
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Kermit Gosnell, Late Term Abortion Provider, Found Guilty Of Three Counts Of Murder
Dr. Kermit Gosnell, a Philadelphia doctor whose clinic gained attention for his criminal performance of late-term abortions, was found guilty yesterday of three counts of first-degree murder. He had been accused of killing four babies and one woman, but was found guilty of only three of the babies' deaths.
Gosnell, 72, operated an… More »


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Slither with a hungry snake, and chomp on cute chicks! You can play in a variety of locations, including Green Garden, Plain Park, and the Waterfall. The objective is to eat all of the chicks in each level. Use the navigator to guide you towards your prey!
Snaket is one of our selected Snake Games. Also very popular on this website right now are Wheely 7, Snack Classic, Doodle God 2 and Hydro Storm 2.
Report game as broken
Cancel report of broken game


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King Avenue Hosts John Shore
Sunday, October 2 at 7:00pm King Avenue United Methodist Church Changing the Conversation Speaker Series "5 Truths About the Bible (And What They Tell Us About LGBT People)," John …Read More
John Shore at OSU October 2
Sunday, October 2 at 4:30pm OSU Union - Cartoon Room Changing the Conversation Speaker Series "10 Ways You can Change the World" John Shore Every person deserves a a …Read More
Fall Lit Club
The Lit Club meets at 7pm on the last Sunday of every month except June and July. The only requirement to participate is a love of reading. For more information contact John Keeny at …Read More
October KACYFM Ministries
King Avenue Children, Youth and Family Ministries, October 2016 Sundays, 9am & 11am Worship Connections Classrooms L4 & L5 Children's Hallway, Lower Level Sunday mornings, 10:00-10:45 …Read More
LGBTQ Inclusion
Our Mission We invite, welcome and honor all people and nurture them to grow in service with Christ. Our Commitment to LGBTQI Inclusion: King Avenue UMC is a member of the Reconciling Ministries Network, which …Read More
Who Are the People in your Neighborhood - Art Gallery
Who are the people in your neighborhood? King Avenue Neighborhoods Evolving Art Exhibit is in the Art Gallery now through October. We want to see YOU in your neighborhood. Bring in a photo of yourself or …Read More
Spectrum Fall Happenings
Spectrum GLBTQI Ministry is looking forward to many events this fall. Please make a note in your calendar and join us. September 25 - All Church Luncheon October 2 - King Avenue hosts …Read More
Coming Out with God
Coming Out with God Mondays, September 26 - October 17 7:00 - 8:30 The Coming Out with God (COWG) support group will continue for another session. Encourage others in the LGBTQ community with this unique, …Read More
New Life Winter Coat Extravaganza!
Winter Coat Extravaganza October 1 - 23 New Life Methodist Church is having its annual Winter Coat Extravaganza. These coats will be distributed through their clothing ministry. Their goal is to have a warm coat for …Read More
Kingsgate Campus Group - Fall Happenings
Kingsgate Student Ministry Welcome students for another great year! After a summer of organizational and weekly meetings, an exciting year is in store. John Shore - "10 Ways You Can Change the World" …Read More
Mens' Bible Discussion
Men's Bible Discussion Half Truths John Keeny will lead a noon five week Bible group for men beginning October 5 through November 2. The class will discuss Adam Hamilton's Half Truths, sayings you think are …Read More
Hubbard Reading Buddy Training
Hubbard Reading Buddy Training Sunday, October 9 School is back in session, and the Hubbard Elementary School Partnership is looking for more Reading Buddies. There will be an information and training session for …Read More
UMW Fall Rummage Sale
UMW Fall Rummage Sale October 14 and 15 Mark your calendar for the United Methodist Women's Fall Rummage Sale on October 14 and 15! Sale revenue supports mission projects in local, national, and international …Read More
Mexico Mission Trip Sign-Ups
Mexico Mission Trip Have you ever thought of taking a mission trip to the Casa de Misericordia Orphanage in Piedras Negras? You should! It's a wonderful chance to share God's love with some amazing children. …Read More
When Kids Come Out
"When Kids Come Out" – A Four-Session Class for Parents of LGBT Children Wednesdays, 7:00 pm - 8:30 pm. October 19 - November 9 Worthington United Methodist Church What …Read More
W.R.A.P. - Find Your Tools for Wellness
Find your tools for wellness with Wellness Recovery Action Planning (WRAP) A self-management program for any mental wellness challenge Thursdays, 6:00-8:00 October 20 - December 1 at King Avenue …Read More
Columbus Marathon Traffic & Worship Info
Columbus Marathon on Sunday, October 16 Traffic and Worship Information Sunday, October 16 is the Columbus Marathon. Although it is possible to get to King Avenue on Sunday morning for worship, many parishioners find it …Read More
Young Adults - September
Open Shelter Lunch Packing: If you are available on Thursday, September 15th 6-6:45pm, please consider coming to the Church to assist in making 200 lunches for the Open Shelter. We will begin promptly at …Read More
NNEMAP Grooming Kits
NNEMAP Grooming Kits NNEMAP is once again collecting grooming kids. This year we are asking our supporters to fill a 2-gallon zip-lock bag with 9 items. The list of items is attached to the bag. Bags will …Read More
Cross Currents
Cross Currents Sundays, 10:00am September 11 - October 30 CrossCurrents is an adult study for individuals and groups that want to apply their United Methodist beliefs to current faith issues and world events. With topics …Read More
September 2016 Carillon and Calendar
It's a very busy fall at King Avenue! Scroll through this list to see the upcoming events for the many ministries in our church. Download the September Carillon Newsletter Download the September …Read More
Transgender Day of Remembrance
Transgender Day of Remembrance Friday, November 18 King Avenue Sanctuary Time TBA The Transgender Day of Remembrance ceremony will be held at King Avenue on Friday, November 18. All are welcome to attend this …Read More
Worship Connections
Worship Connections Worship Connections begin on September 11th! This children's ministry allows our King Avenue Kids the opportunity to explore God's love, sing songs and spend time learning together …Read More
Your Giving Makes Ministries Happen!
A Message from the Finance Committee Thank you for your generosity! To get an appreciation for what you make possible, see all that's written in the Carillon, on our Facebook page, on our website, and spoken about …Read More
King Avenue Online Giving
Giving Made Easy! King Avenue is pleased to announce the expansion of electronic options for making regular offerings. As before, contributions can be debited automatically from your checking or savings account; …Read More


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Everything you need to know before Kenny Chesney's show at Miller Park
Yes, Milwaukee music fans, it's been a long, long time.
Miller Park hasn't hosted a full-blown music concert since Farm Aid in 2010, and memories of just how stadium shows operate may be fading. »Read Full Article(2)
Chieftains keep it simple and fun at Pabst Theater
After 52 years, you might expect the Chieftains to give a phoned-in performance.
You'd be wrong. »Read Full Article(1)
Theatrical Tendencies' 'Temperamentals': Being gay in '50s
Late in playwright Jon Marans' "The Temperamentals," the lead character addresses the audience, challenging the notion that the gay liberation movement began with those now-legendary 1969 riots outside a Greenwich Village bar.
"Before Stonewall," gay activist Harry Hay (Mark R. Neufang) tells us, "an even braver bunch of us stood up to the plate — before there even was a plate!" Led by Hay and his lover in Los Angeles, these brave pioneers called themselves the Mattachine Society. »Read Full Article
6 chefs, 5 courses, 5 beers as dinner series kicks off at Hinterland
The first in a series of beer dinners that bring together some of the city's top chefs will be March 25 at Hinterland Erie Street Gastropub.
Chefs cooking for the Best of Milwaukee beer dinner are Justin Aprahamian of Sanford, Daniel Jacobs of Odd Duck, Paul Zerkel and Lisa Kirkpatrick of Goodkind (due to open in spring in Bay View), Dan Van Rite of Hinterland and Matt Kerley of Blue Jacket. »Read Full Blog Post
'Cesar Chavez,' the movie and the book
"Cesar Chavez" a new feature film that tells the story of the famed farm worker organizer and civil rights leader, opens soon in theaters across the country.
The movie bio-pic stars Michael Peña, and Rosario Dawson. »Read Full Blog Post
Brewers still working out details on Hank the dog's new home
Phoenix — The question persists: Where will home be for Hank, the Brewers dog?
For the answer, apparently, we'll have to wait a little longer. »Read Full Article(18)


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Top Definition
when you are in an enclosed area that is very smokey but it's not from weed as usual it's from a cigarette.
"Dude, crack a window we've got a fuckin native hotbox going down!"
автор: Jill*Smoke2Joints* 27 февраля 2008
Trending Now
Can you define these popular missing words?


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News Picks : North Korea claims hydrogen bomb success, experts skeptical
Guardian: Earlier today researchers around the world detected a magnitude 5.1 artificial earthquake originating in North Korea. Afterward, the country's state media announced that North Korea had successfully detonated a hydrogen thermonuclear bomb. However, judging by the strength of the seismic activity that was detected, international experts say the test more likely involved a traditional plutonium or uranium nuclear device. To determine exactly what type of detonation occurred, the Comprehensive Nuclear-Test-Ban Treaty Organization will use its monitoring network to look for radionuclide indicators in the atmosphere.


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I will make clean and bright video intro with your logo for $5(37)
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also I can add tagline - if you need it!
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- more gigs you can find here: https://www.fiverr.com/taplon
Check out my Gig Extras
- Basic Gig Quantity


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University News
Start Smart with a Graduate Student Library Orientation
Get to know the powerful tools and unique resources your Libraries have for graduate students. Learn about library spaces and services, and discover resources specific to your discipline. This orientation will provide a great overview of how to use the library and make sure you are ready for that first research project.
There are six sessions to choose from:
Thursday, August 18, 5-6pm
Friday, August 19, 11:30am-12:30pm
Thursday, August 25, 4-5pm
Friday, August 26, 11:30am-12:30pm, 2-3pm, and 3-4pm
These orientations fill up early so please RSVP to reserve your preferred session.
Be sure to check out our research guide, "What Graduate Students Need to Know " for more information, and feel free to contact a librarian to schedule a one-on-one research consultation at any point during the semester.
Are you living in a filter bubble? Web searching, privacy, objectivity, and GW Libraries search.
This past spring, four of us here at GW Libraries had the privilege of attending the 2016 Code4Lib conference, featuring a wide variety of talks and discussions relevant to anyone interested in technology in libraries, archives, and museums.
The closing keynote was given by Gabriel Weinberg, the CEO and Founder of DuckDuckGo. If you're not familiar with DuckDuckGo, it's a search engine committed to not tracking you.
Tracking Your searches: Good and Bad
When you search using Google or other engines that track you, there's the obvious privacy issue around the company recording of all of your searches, but there's another aspect (let's refrain from judging it for the moment) which is that it affects the results of your search. Sometimes you may actually want that, but sometimes you don't. But let's first see when and why this happens.
You and I May Get Different Search Results
I'm going to use Google as an example, but this could apply to Bing, Yahoo, and other popular search engines as well.
Search engines that track you incorporate several factors into determining which results you see. If you're logged in to Google and haven't turned off the personalization settings, to the extent they can be turned off, then Google bases your results, and their rankings, on your previous searches (and possibly other information it knows about you from terms in your email, etc.) to try to present you with results it thinks you're likely to want and to click on. Other factors it takes into account include your location based on your IP address.
When you're hungry and want to quickly find something to eat nearby that you might like, you might want results that are localized and perhaps even take into account what it knows about your preferences. But when you're doing research for a paper, you may simply want the most objetive, consistent search results possible.
Here's an example: A Google search on "Obama" yielded slightly different results when I was not logged in to a Google account, versus when I was logged in to my (personal) Google account. The top news links were different: NBC, BBC, ABC, versus NBC, CBS, BBC; and a New York Times link was ranked considerably higher when not logged in, versus logged in:
One result of personalized results is the phenomenon referred to as the "filter bubble," a concept coined by Eli Pariser in his 2011 book. A filter bubble means that you're presented with results that tend to further reinforce your existing preferences, beliefs, and opinions. There is some controversy around the extent of the effets of this, but it has been a topic more in the forefront lately, particularly when it comes to social media and how platforms such as Facebook and Twitter determine which news items to prioritize in your feed.
Privacy, Tracking, Personalization and Other Search Engine "Features"
Let's check Wikipedia to get a rough sense of which search engines employ tracking, share information with third parties, and which don't:
From https://en.wikipedia.org/wiki/Comparison_of_web_search_engines#Digital_rights as of June 30, 2016:
Is the knowledge that your information might be shared with third parties, and that the search engine might be at least attempting to modify your browser settings ("browser hijacking"), worth the tradeoff of the benefits you derive from using those search engines? That's a personal choice, but it might be worth your while to try out a variety of search engines, paying attention to which track and which don't track.
Can't I just use Incognito Mode?
Incognito Mode seems to be somewhat misunderstood by many people. Incognito Mode is a browser feature that refrains from saving your browsing history and cookies in the browser itself, but if you're logged into Yahoo, Google, etc. within the incognito-mode window, they're still saving your searches on their side, and results may still incorporate your location and/or IP address.
Trackless Search Engines
One solution to concerns about privacy and objectivity is to consider using a search engine which doesn't track you. One of these is DuckDuckGo, which we mentioned earlier.
Libraries and Privacy
GW Libraries follow in the long-held library tradition of respecting and protecting patrons' privacy as well as providing objective search results when you use our research tools:
We won't share your circulation records, and records of electronic materials that you accessed.
We don't track you! When you search through the library web search interfaces, you will get the same results as anyone else in the GW Community, and the GW search engine is not tracking or saving anything about you. We wrote it, and the code that runs it is open source, so you can see it for yourself on github!
And last but not least, you won't get advertisements!
The only factor that can change your search results is whether you're using the GW Libraries search interface from an on- or off-campus IP address. This is because some of the resources, usually resources that GW pays to provide, are available to you as a member of the GW community, but not to the general public.
We do anonymously log search queries that come through the "All" tab (fondly known as the "Bento" search). The queries are anonymous; they are not associated with any user or even an IP address. We use these to better learn about our users are searching for - particularly the most popular searches - and we use what we learn to improve the research tools we provide.
Here's an example of a view that we as GW Libraries staff can see. Note that there's no information about who submitted each search:
More on the GW Libraries "All" search in a future blog post!
The Bottom Line
If you're using a search engine to try to survey and locate web content for research purposes, you probably want the most objective results and rankings possible, un-influenced by your personal search history and possibly even un-influenced by your location. Educate yourself about search engine choices so that you can make a thoughtful choice about which one to use.
Some further reading:
GW Libraries' "How Do I?" page on using Google Scholar: https://library.gwu.edu/howdoi/googlescholar
The Library Freedom Project, working to protect digital privacy and freedom in libraries: https://libraryfreedomproject.org
Google's privacy policies https://www.google.com/intl/en-us/policies/privacy/#infochoices
More about DuckDuckGo: https://duckduckgo.com/about
VISION Magazine 2016 Now Available in Print & Online
We are proud to announce that the annual magazine of the GW Libraries, VISION, is now available in print and online.
In this edition, we explore how the GW Libraries contribute to student and faculty scholarship through collaborations to create new software, build databases, perform statistical analyses, create 3-D models, manage and visualize their research data, and more. You can also find out how showcasing faculty scholarship, using the power of crowdsourcing to understand history, and providing a 24/7 "amazing space for students" are all part of the daily work of the GW Libraries.
Growing a Global Perspective
Death in America's Shadows
Mastering the Haiku of Filmmaking
Gelman & Eckles Closed for Independence Day
Gelman and Eckles Libraries will be closed on Sunday, July 3 and Monday, July 4 to celebrate the Independence Day holiday.
Gelman will reopen at 7 a.m. on Tuesday, July 5. Eckles will reopen at 9 a.m. on Tuesday, July 5.
Can Writing a New Constitution Improve Democracy?
Top Documentary Festival a Showcase for American University Faculty
SIS Alumni Relations Office receives award for career development program
Action plan signed on women's economic empowerment
2016 Kogod Made in America Auto Index
Reporting Boot Camp
Student Awarded Ruby's Artist Project Grant
Playing for a Cure
Playing for a Cure
Documents Tell the Story of Enslaved People at the Columbian College
The year was 1847 and slavery was legal in the District of Columbia, although it was the site of significant anti-slavery activism. Two enslaved men, known only as Abram and John, were owned by Capt. Haynes of Virginia and brought to Columbian College to assist him in his work as the college's steward. Columbian College student Henry J. Arnold provided Abram with $14 and a letter for an attorney with the intention that Abram would file a lawsuit to win his freedom. For this act of bravery, Arnold was expelled from the college.
While the Arnold Case was not completely forgotten in the history of GW, it has remained largely obscure or else apocryphal to both scholars and the general public. Thanks to collaboration between DCAAP and the GW Libraries' digital services unit, the University Archives have now made available to scholars a cache of documents that illuminates this situation. The documentation consists of drafts and copies of letters written by Columbian College's then-president, Joel S. Bacon, to Arnold, his family and others who inquired or appealed to Bacon about the matter. With this critical documentation, the story of enslaved people at Columbian College can now be more fully told.
Read more about this incident and the about the documents in a post by University Archivist Christie Peterson.
Studio Art Student Award Winners Announced
Alumnus Madden Takes Schorr Journalism Prize
Opening Celebration of the Corcoran Archives
Wednesday, June 15
5 - 6:30pm
Special Collections Reading Room (704)
Please join us as we celebrate the opening of the Corcoran Archives in its new home at GW Libraries Special Collections Research Center. Selections from the collection will be on display in the reading room and light refreshment will be served. Archivists will be available to answer questions and discuss what is known about the archives now and what may yet to be discovered in them.
The Special Collections Research Center is located in suite 704 of the Gelman Library. Photo ID is necessary to gain entry to the Library.
Nearly a decade has passed since the archives of the Corcoran Gallery and the Corcoran College of Art and Design—now the Corcoran School of the Arts and Design—were accessible to researchers. In June, nearly 2,000 boxes of historical documents and hundreds of thousands of architectural drawings, exhibition posters, photographs and oversized ledgers were donated to the George Washington University from the Corcoran board of trustees.
The Corcoran archives document the life and vitality of one of D.C.'s oldest cultural heritage institutions and provides nearly 150 years of insight into the history of American art museums and art education, from its founding in 1869 through 2014 when the historic agreements between the Corcoran, GW and the National Gallery of Art were finalized. The archives will be available to the public at the Special Collections Research Center in Gelman Library.
Learn more about the archives and how to access them on the Corcoran Archives webpages.


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You may also like these...
Sigma EX DC 10-20mm F3.5 HSM Nikon Fit
Nikon AF-S 14-24MM F2.8 ED
Superb Image quality and performance
The optical construction of 18 elements in 13 groups, including for the first time an XGM (eXpanded Glass Moulded Aspherical) element within the front group and several LD (Low Dispersion) glass elements, assures that this lens delivers outstanding image quality throughout the entire zoom range and from corner to corner.
VC stabilization Tailored for Ultra Wide and Low light
Recognising the need for an image stabilization solution for fast f2.8 ultra-wide-angle lenses, Tamron have tuned their VC (Vibration Compensation) system specifically for the zoom range of this lens to give excellent performance even with low light and slow shutter speeds.
BBAR and eBAND lens Coatings
Tamron have paid particular attention to their BBAR Broad-Band Anti-Reflection) which has been refined further for this Ultra wide lens. Coupled with the eBAND coatings this results in crisper, crystal-clear images with ghosting and flare being efficiently eliminated.
Water and Dirt repellent Fluorine coating
The front element has a special Fluorine coating to repel water and dirt in order to keep it as clean as possible and make it easy to clean resulting in clearer sharper images even when in challenging environments.
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The 9 blade curved aperture construction gives amazing smooth backgrounds within the out of focus areas of your image when using wider apertures due to its near circular form.
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Unique built in Lens hood design
This unique lens hood provides effective shading of the front lens surface over the entire zoom range to minimize flare. It utilizes the unique characteristics of the optical system in which the front lens group recedes as you zoom in from 15 mm to 30 mm.
A double-hood structure has been also incorporated into the lens design for additional strength
Lens Construction 18 elements in 13 groups Minimum aperture (W) F22 Filter size n/a Angle of view (35mm equivalent) 110 - 71° Minimum focusing distance 28cm Dimensions (Diameter x Length) 98.4 x 145mm Number of diaphragm blades 9 Maximum magnification ratio 1 : 5 Weight 1100g
Additional Information
|Box Contents:
|Lens, Lens Hood (fixed), Instructions


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Rent A Gentleman Instructed in the True Principles of Religion, with a Full Confutation of Atheism and Latitudinarianism the Second Part Written For 1st edition today, or search our site for other textbooks by William Darrell. Every textbook comes with a 21-day "Any Reason" guarantee. Published by BiblioBazaar.
Need help ASAP? We have you covered with 24/7 instant online tutoring. Connect with one of our tutors now.


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Reversing overflows can be complex, but in a pinch, mind these steps before the October 15th deadline.
Entries by Brian Dobbis
Unwinding Excess IRA ContributionsSeptember 23, 2016 11:40 AM by Brian Dobbis
SIMPLE IRA Rules of the RoadSeptember 15, 2016 12:58 PM by Brian Dobbis
While SIMPLE IRAs have come a long way in 20 years, the rules may sound complex at times. Here's a refresher on salient guidelines.
State-Run Retirement Plans: Now in Three FlavorsSeptember 8, 2016 4:30 PM by Brian Dobbis
The Department of Labor recently clarified when a state-run plan would not be covered by ERISA. Whether they catch on and can compete with far more popular offerings remains to be seen.
IRAs and 401(k)s: Rollover Help from the IRSSeptember 1, 2016 12:45 PM by Brian Dobbis
The IRS has announced a new and expedited process that offers relief to taxpayers who have missed the 60-day deadline for rolling over distributions from employer-sponsored plans and IRAs.
IRAs versus 401(k)s: More Rules You Should KnowAugust 25, 2016 3:05 PM by Brian Dobbis
Back by popular demand: rule differences we didn't cover last week.
IRAs versus 401(k)s: Key Rule DifferencesAugust 18, 2016 10:30 AM by Brian Dobbis
IRAs and 401(k)s both have unique tax benefits, and different rules. Understanding them can help you manage your retirement savings.
Self-Directed IRAs ExplainedAugust 11, 2016 11:26 AM by Brian Dobbis
The rules and process may be quite a maze, but with the right advice, investors can save for retirement with a wide range of alternatives.
Naming IRA Beneficiaries: Don't Rely on Your WillAugust 4, 2016 4:12 PM by Brian Dobbis
Failing to name beneficiaries on retirement accounts is one of the biggest mistakes investors can make. Don't forget that important step in the planning process.
Tips for Roth IRA HeirsJuly 29, 2016 3:09 PM by Brian Dobbis
Anyone who has inherited a Roth IRA probably has many questions. Here are some answers.
Explore the HSA by Rolling Over Your Retirement SavingsJuly 22, 2016 3:18 PM by Brian Dobbis
Have you taken full advantage of health savings accounts (HSAs)? The tax savings can help defray the cost of living and getting sick.


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When Lori Cuno started her career more than a decade ago, she planned to go into elementary education. A love for helping those in need even led her to take on a couple of long-term substitute positions in the world of special education while completing her master's degree in counseling.
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Canyon Courier is your source for local news, sports, events, and information in Evergreen, Colo, and the surrounding area.


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Your good credit, down payment, and increase in income will definitely be helpful. I first need to understand your scenario in more detail; such as monthly debts and what y ... more
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Addenda to volume 12: Minutes of the Records and Accounts of the Chamber
The History and Topographical Survey of the County of Kent: Volume 12
of the Chamber Minutes of the Records and Accounts of the Chamber [1234-1448] ... in the Chamber of Canterbury, of Transactions in that City, during the Time ... chapel, in St. Dunstan's church,...View result
Star Chamber Reports: 9 Charles I
Historical Collections of Private Passages of State: Volume 3, 1639-40
Star Chamber Reports 9 Charles I Pas. 9 Car. Wicksteed versus Constantine. Maintenance. The Defendant Constantine disbursed mony toward ... were dressing: And for this the Defendants were all committed to the...View result
Plate 103: Hampton, Hampton Court, Overmantels
An Inventory of the Historical Monuments in Middlesex
Plate 103 Hampton Hampton. (2) Hampton Court. Overmantels. Late 17th-century. King's First Presence Chamber. King William's Bedroom. King's Audience Chamber. King's Private Dressing Room. An...View result
Plate 104: Hampton, Hampton Court, Royal Chambers
An Inventory of the Historical Monuments in Middlesex
Plate 104 Hampton Hampton. (2) Hampton Court. Fireplaces. Late 17th or early 18th-century. George II's Private Chamber. King's Dressing Room. Queen's Private Chamber. Queen's Bedroom. An...View result
Plate 109: Hampton, Hampton Court, Iron Firebacks
An Inventory of the Historical Monuments in Middlesex
Plate 109 Hampton Hampton. (2) Hampton Court. Iron Firebacks. Queen's Audience Chamber. Cartoon Gallery. King's Dressing Room. King's Drawing Room. King's Audience Chamber. An Inventory of the...View result
House of Commons Journal Volume 8: 21 June 1661
Journal of the House of Commons: Volume 8, 1660-1667
in the Speaker's Chamber, at Two of the Clock in the Afternoon: And to send ... and Dressing of Leather, was this Day read the Second time. Resolved, upon the Question, That the said Bill for better Tanning,...View result
The bedchamber: Women of the Bedchamber 1702-1714
Office-Holders in Modern Britain: Volume 11 (Revised), Court Officers, 1660-1837
The bed-chamber woman came in to waiting before the queen's prayers, which was before her majesty was dressed. The queen often shifted [i.e., changed her clothes] ... at her dressing. When the...View result
House of Commons Journal Volume 1: 25 June 1604 (2nd scribe)
Journal of the House of Commons: Volume 1, 1547-1629
in the Exchequer Chamber. The Exchequer-men to attend. Obedience of the Subject. ... Clothing. The Bill to avoid all Deceits in Clothing, and in dying and dressingView result
House of Commons Journal Volume 1: 10 May 1624
Journal of the House of Commons: Volume 1, 1547-1629
Chamber. Cloth Trade. Speaker went out of his Chair, and the House fell ... of Merchant Adventurers Privilege, with dyed and dressed Cloths. Resolved, Upon ... of their Privilege, with dyed and dressed...View result
Roger Whitley's Diary: June 1687
Roger Whitley's Diary 1684-1697 Bodleian Library, Ms Eng Hist C 711
dressed my leg; brother Peter came to see me; then Sir Edward Wood ... Row came to me ; stayd not long. 2. Thursday, dressed my leg; my son's ... dressed my leg; then came Edisbury...View result


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© 2011 Chris Armytage, Fine Art Photography, Daylesford, Victoria.
Inspired by RJHeller's …"light" …
Composite of 2 photographs taken with a Panasonic FZ20, edited and layered with textures from my own stock photography in Photoshop CS3. The trees are were taken in Autumn in the Wombat Botanical Gardens in Daylesford, Australia, where you will find some fine examples of the few remaining Dutch Elms in the World.
FEATURED High Quality Images, Permanent Feature Page Forever Searching for the Light 10 June 2011
FEATURED in Art Universe, Permanent Feature Gallery – Grunge 15 July 2011
FEATURED in Nature and Man, 2 August 2011
FEATURED in Colour me a Rainbow, 22 August 2011


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is still very sexy even at her age. She got divorced long ago and George is the
only her son. He just turned 18. They are great friends even though many teenagers
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Amanda�s sons grew up it was very painful for her to watch them having fun at
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Sons often joked that she�s she only woman in the world they will ever love. Once
they had pretty frank conversation about sex and Amanda said desperately she�s
not able to explain everything they ask her about but she�s able to show! That
was nasty but her sons took her right. It was their first incest experience so
they recorded it on video.
grew up without mother. She died when Helen was a just small kid. Her father Artur
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he kissed her lips while she was sleeping. She woke up and finally realized how
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enjoyed it with Artur. She got her cunt eaten out as never before and then stuffed
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Boy, 6, dies days after South Carolina school shooting
Jacob Hall had been fighting for his life at a hospital since the shooting Wednesday at Townville Elementary School.
Jacob Hall had been fighting for his life at a hospital since the shooting Wednesday at Townville Elementary School.
Here's what is known about the investigation into a commuter train crash that killed one person and injured more than 100 others Thursday in…
A man who police say abducted and sexually assaulted a 9-year-old girl in northern Ohio has pleaded not guilty to rape and kidnapping charge…
A boil advisory that was issued Thursday for parts of Champion following a water main break has now been lifted.
The latest weather forecast for Youngstown, Warren, Austintown, Boardman, Sharon, Lisbon and East Liverpool.
It occurred around 5:30 p.m. on Friday. No one was injured.
Almost a year later, police say the case is no closer to being solved.
"Did Crooked Hillary help disgusting (check out sex tape and past) Alicia M become a U.S. citizen so she could use her in the debate?" read …
The event comes a day after two videos of the shooting were released by authorities.
Protesters held placards calling for justice for Keith Lamont Scott, who was shot by a police officer outside his townhouse complex Sept. 20…
"Traficant: The Congressman of Crimetown" will play Saturday at 4 and 7 p.m. and Sunday at noon and 3 p.m.
School officials say only a handful of East High students are disruptive, but those few give the school a bad reputation.
The Mill Creek Wildlife Sanctuary has more than 225 species of birds, like shorebirds, wading birds and wood ducks.
The event was put on by the Autism Society of the Mahoning Valley with help from local photographers.
Philadelphia's police commissioner says nine officers fired a total of 109 shots at a man who went on a stabbing rampage.
Customers got their first looks — and first tastes — of the Asian-American restaurant at 11 a.m. Saturday morning.
A Pennsylvania man has been sentenced to at least two months in jail for posing his 6-month-old daughter with a handgun.
Halloween and fall activities in Mahoning, Trumbull, Columbiana and Mercer counties.
WKBN has covered these arrests in and around the Mahoning Valley in the month of September 2016.
Aviators use big plays in come from behind win
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Racism is the lowest, most crudely primitive form of collectivism. It is the notion of ascribing moral, social or political significance to a man's genetic lineage ... Which means, in practice, that a man is to be judged, not by his own character and actions, but by the characters and actions of a collective of ancestors.
- Ayn Rand
Thursday, 14 October 2010
QUOTE OF THE DAY: Racism
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5. Say what you mean, and mean what you say.
6. Off-topic grandstanding, trolling and spam is moderated. (Unless it's entertaining.)
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Gone are the days when people used to use second generation GSM Mobile phones. Today's technology has revolutionized the traditional and limited feature mobile phones.
Definitely, the mobile phone industry has grown with time. new technologies, functions and features are packed with high tech phones. You can now video call, send pictures, reply to your mails and get print out for your documents – all of this straight from your phone.
Well all these functions are actually not installed directly to the phone, they are different features of various applications that you download from a particular app store. For instance, for sharing documents and pictures we use SHAREit; for video calling Skype and FaceTime are the best apps and there are many more to look upon.
If we talk about Movie downloader, then you all should know that these apps got introduced to the digital world quite late in 2000. While they are few in number, Movie downloaders have won hearts of many in these few years. ShowBox app is one of those.
ShowBox HD
ShowBox HD is a movie downloader app that enables its users to download unlimited movies and videos in one go. It is especially designed for Android devices but as the new trend goes, interested users can download ShowBox HD on any device.
ShowBox HD and many other applications like TubeMate, SnapTube, MegaBox, PlayBox, PlayView, CinemaBox HD etc are not available on the Google play store. Therefore, users need to download or share the app from another device via SHAREit or vShare. They can even directly download the app from different website portals like Mobango, 9apps, apkmirror, apkpolice or uptodown. These portals will provide you with the best links available for the applications.
Talking about ShowBox HD, it goes without saying that it is one of the best app for downloading movies. Let's discuss its features right through the starting to get an better idea of what exactly this app will do for you :-
- Watch movies on your phone, PC or Tablet for free.
- Download movie and songs at a high speed with lowest internet allowance used.
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All said and done, ShowBox HD is facing some issue with its downloading and network process. But this should not deter you from downloading this wonderful app. The work is under process, ShowBox HD team will come with an solution to it as soon as possible. No doubt it is still, the best application that can entertain you till the end. You all should try it!


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Andros Townsend celebrates Tottenham's win at Old Trafford with banner at the darts
Tim Sherwood's excellent start to his coaching career at Spurs, has come with the back drop of a large injury list.
The likes of Jan Vertonghen, Younes Kaboul and Sandro were injured when Sherwood took over from AVB.
Whilst Andros Townsend, Jermain Defoe, Gylfi Sigurdsson and Paulinho have all picked up injuries whilst Sherwood has been in charge.
Townsend was injured during Sherwood's very first game in charge and would no doubt be a good addition to a Spurs side that is counter attacking with real style away from home.
The England winger appeared at the PDC World Carts Championship on Wednesday night and appeared on the Sky Sports coverage – see video below.
He was also cornered by former cricketer and Manchester United fan Ronnie Irani, so held up the banner celebrating Tottenham's win at Old Trafford, in the picture above.
Watch Live Stream
Latest on Andros Townsend
- Andros Townsend thanks Newcastle fans on Instagram after signing for Crystal PalaceJuly 1, 2016
- Marseille's Michy Batshuayi & Newcastle's Andros Townsend close to Selhurst Park moves [Papers]June 28, 2016
- Redman TV anlayse rumours linking Newcastle's Andros Townsend to Liverpool (Video)June 21, 2016
- Liverpool & Man City target England winger [Telegraph]June 21, 2016
Latest on Tottenham
- Spurs players celebrate CSKA win on InstagramSeptember 28, 2016
- Hilarious: Georges-Kevin N'Koudou praises his own performance [Tweet]September 27, 2016
- Heung-Min Son scores again as Spurs beat CSKA Moscow (Video)September 27, 2016
- Spurs (Son) and Leicester (Islam Slimani) record big wins in the Champions LeagueSeptember 27, 2016


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Is it possible that BA may place the A380 on a domestic route for training flights ?
Does the infrastructure at the major northern UK airports allow for this ?
Obviously MAN has A380 service but probably EK uses different terminal ?
|Quoting finnishway (Reply 2):
Didn't they have a plan flying training flights to Madrid?
|Quoting 1400mph (Reply 5):
Yeah but if I was flying between LHR and MAN I'd rather try out a brand new A380 instead of an old Aer Lingus A320.
|Quoting OA260 (Reply 9):
Nothing wrong with any of the numerous ''old'' Aer Lingus aircraft I have flown on. What Reg's exactly where you on and what did you find at fault with them?
|Quoting eicvd (Reply 8):
Im sorry to disappoint you but there is nothing wrong with an 'old' EI A320
|Quoting anstar (Reply 10):
No not naughty - just predictable!
|Quoting RWA380 (Reply 11):
For a short hop like that an A380 would be a blast, a true A.netters dream. Nothing wrong with the A320, but if you have a choice, Come on, we'd all take that opportunity, if they say they wouldn't. then they are not being entirely forthcoming.
|Quoting 1400mph (Reply 12):
I would of thought so too !
|Quoting 1400mph (Reply 12):
Just a bit of fun anstar.
|Quoting 1400mph (Reply 12):
t's very easy to attack either VS or BA if you so choose
|Quoting by738 (Reply 16):
|Quoting by738 (Reply 16):
PIK is not A380 capable
|Quoting skipness1E (Reply 19):
There have been more than a few threads on this already where MAN was ruled out as the only A380 gate is at T1 and BA fly from T3
|Quoting bongodog1964 (Reply 20):
Surely domestic routes aren't long enough to provide realistic crew training, barely time for a quick trip down the aisle handing out deli boxes and a cup of coffee. I would have thought that a route of at least two hours would be required to allow the cabin crew to learn anything meaningful. Of course to the flight crew its take offs and landings that matter, so the shorter routes the better.


text:
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#41 - gilfhunter
Reply +7(03/28/2013) [-]
The 90s called..they what their jokes back
#47 to #41 - comicsjoey **User deleted account**
Reply +4(03/28/2013) [-]
they what their jokes back?
#49 to #47 - gilfhunter
Reply -2(03/28/2013) [-]
Blonde jokes, blondes being stupid- generally a product of the 90s...it seems to be something that just wont die...
#69 to #51 - gilfhunter
Reply 0(03/29/2013) [-]
why thank you grammar fairy. I sometimes forget that Funnyjunkers are incapable of critical thought when it comes to fairly minor mistakes. I will keep that in mind
#50 to #49 - comicsjoey **User deleted account**
Reply +3(03/28/2013) [-]
read the sentence you just wrote before.
read the sentence you just wrote before.


text:
Archive for Tuesday, May 14, 2013
Chieftain softball falls to Bonner Springs
May 14, 2013
The Tonganoxie High softball team's season came to an abrupt end Monday with an 18-3 loss to Bonner Springs in Class 4A regional action at Eudora.
The Chieftains (11-8), who split with the Braves (6-12) to end the regular season, jumped out to a 2-1 lead at the end of the first inning. The lead was short-lived, though, as BSHS reeled off five runs in the top of the second. THS got one run back in the home half of the inning, but following a scoreless third, the Braves erupted for 12 runs in the fourth inning.
The mercy rule went into effect when the Chieftains failed to score in the bottom of the fourth.
THS managed just four hits off of Bonner Springs pitching. Juniors Lainy Walker and Morgan Oroke each went 1-for-1 with a run batted in. Senior Jaycee Knipp and junior Caly Ingle-Maxwell each went 1-for-3. An RBI double by Knipp was the team's only extra-base hit.
Oroke suffered the loss in the pitcher's circle, allowing 12 runs in two innings. Freshman Sammi Bates didn't fare much better, giving up six more runs in two innings of relief.
Despite the loss, the Chieftains ended their season with a winning record for the first time since 2007.
Bonner Springs 18, Tonganoxie 3
Leading hitters: Lainy Walker 1-1, RBI; Morgan Oroke 1-1, RBI; Jaycee Knipp 1-3, 2B, RBI; Caly Ingle-Maxwell 1-3.
More like this story
- OVERTIME THRILLER: Tonganoxie football outlasts Eudora, 46-40
- Turnovers doom Tonganoxie football in district loss
- Tonganoxie football staves off Perry-Lecompton rally for first victory
- Tonganoxie football blanks Bishop Ward, 42-0
- Tonganoxie football holds on again after big lead for 2nd victory of season
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text:
Gina Gershon and Beck had a relationship.
Gina Gershon is a 54 year old American Actress. Born Gina L. Gershon on 10th June, 1962 in Los Angeles, California, United States and educated at Beverly Hills High School, she is famous for The Insider, Showgirls in a career that spans 1985–present. Her zodiac sign is Gemini.
Beck is a 46 year old American Musician. Born Bek David Campbell on 8th July, 1970 in Los Angeles, California, he is famous for Mellow Gold, Odelay, Sea Change in a career that spans 1988–present. His zodiac sign is Cancer.
Help us build our profile of Beck Hansen and Gina Gershon! Login to add information, pictures and relationships, join in discussions and get credit for your contributions.
Help keep Beck Hansen and Gina Gershon profile up to date.


text:
The Colour Purple
League Express editor Martyn Sadler responds to criticism by some Widnes supporters following a comment he made on Twitter last night. Last night I tweeted a comment when I was watching Widnes and Wigan to the effect that I wished
League Express editor Martyn Sadler responds to criticism by some Widnes supporters following a comment he made on Twitter last night. Last night I tweeted a comment when I was watching Widnes and Wigan to the effect that I wished
In this week's League Express, editor Martyn Sadler has paid tribute to Castleford following their fantastic performance against Leeds Rhinos. A week earlier, Martyn wrote about Daryl Powell's exploits in the transfer market. You can read Martyn's thoughts every week
Widnes Vikings star Rhys Hanbury is still leading this season's Albert Goldthorpe Medal table after eight rounds of the Super League competition, following the points allocations for Easter Monday's Round 8 matches. Hanbury received one point for his performance for
The 2015 season contained enough memories to last a lifetime, with triumph and tragedy in equal measure. It was the season in which the RFL claimed that every minute would count, and in Monday's League Express, editor MARTYN SADLER introduces a
MARTYN SADLER, the editor of League Express, admits that he has mixed feelings about the return of Sam Burgess to South Sydney. So now the storm is dying down, and Sam Burgess has arrived back in Sydney to play for
Martyn Sadler, the editor of League Express, wonders whether England coach Steve McNamara has what it takes to make the changes necessary to secure victory against New Zealand at Wigan on Saturday. This is an updated version of an article
League Express editor Martyn Sadler gives his reaction to England's performance in a disappointing 9-2 defeat by New Zealand. England's defence was outstanding, conceding just one try, and that was probably a try that could be excused, having been created by
League Express editor Martyn Sadler wonders whether Sam Burgess might have been better advised to sign for Leeds Rhinos in Super League, rather than returning to the South Sydney Rabbitohs So Sam Burgess is going to return to South
MARTYN SADLER gives Huddersfield Giants some advice before tonight's Super League semi-final clash against Wigan Warriors at the DW Stadium. How do you recover from a match that you looked certain to win, but which was snatched away from your
Luke Gale has won the 2015 Albert Goldthorpe Medal, as was revealed recently by League Express. And the question now is whether Gale's superb form in 2015 will cause England coach Steve McNamara to select him for the international squad


text:
ALERT: Fraudulent user freelancer "onlinesourcee" Ronjon Prince
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Brooklyn, NYUSA
October 20, 2011
During the day.
New York Historical Society; The Museum of the City of New York; The Tenement Museum; the Met.
The Five Points and the Prohibition walking tours.
I am very interested in New York history.
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Solari Energy Announce Distribution Of VoltLogic Inverters
A major benefit of this inverter is the all-in-one solution. It enables the use of available energy sources - renewable and non-renewable - as well as energy storage devices.
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RAPD analysis of environmental, food and clinical isolates of Campylobacter spp.
The typing of Campylobacter is relatively poorly developed compared to that of the Enterobacteriaceae, and new molecular methods may provide useful approaches. The polymerase chain reaction was used to amplify randomly primed genomic DNA from Campylobacter isolates with an optimised randomly amplified polymorphic DNA protocol. Groups of isolates were analysed from chicken house environmental sources, chicken joints from retail sources, patients suffering from clinical disease and laboratory culture collections. Amplicons were separated by agarose gel electrophoresis, stained with ethidium bromide, and banding patterns captured in a digital form for computer analysis with GelCompar software. The method gave 100% typability and reproducibility for the isolates investigated and proved a useful technique for the epidemiological analysis of Campylobacter. Computer-based analysis of the randomly amplified polymorphic DNA generated profiles allowed relationships between isolates to be studied at the molecular level resulting in some indication of molecular correlates of the origins of isolates.
- Campylobacter
- Randomly amplified polymorphic DNA
- Computer-aided epidemiological analysis
- Molecular typing
1 Introduction
Campylobacter is recognised as the most significant cause of bacterial foodborne infection in developed countries [1]. The number of reported cases of Campylobacter enteritis has risen dramatically since 1977, Communicable Disease Surveillance Centre (CDSC) figures bringing the total laboratory reported UK cases in man to 44 414 during 1994, and 43 902 in 1995 [2]. The reported food poisoning cases are a crude estimation of the true extent of the burden of disease as in addition to difficulties arising from the isolation and characterisation of fastidious Campylobacter isolates, it is conservatively estimated that only 10% of cases of food poisoning are reported [3]. Associated costs run to hundreds of millions of pounds per year in terms of health care and loss of productivity [4].
The sources of foodborne outbreaks are rarely traced. In order to investigate sources and modes of transmission of these organisms it is important to have epidemiological typing methods which can discriminate between different strains but which can reliably and reproducibly recognise the same strain. Current methodologies for typing Campylobacter are poorly developed, and vary in their usefulness with respect to discriminatory power, cost and ease of use, with attention being turned to molecular methods in recent years.
A rapid typing method based upon randomly amplified polymorphic DNA (RAPD) was described [5, 6] and is a modification of the polymerase chain reaction (PCR) in which a single primer able to anneal and prime at multiple locations randomly distributed throughout the genome can produce a spectrum of amplified products characteristic of the template DNA. Thus, the entire genome is potentially accessible to priming and amplification. Detection of polymorphisms by established fingerprinting procedures requires prior knowledge of the target DNA sequence or cloned characterised probes. RAPD however can characterise anonymous genomes with arbitrarily chosen oligonucleotide primers [7]. Randomly amplified polymorphic DNA analysis has been reported for the characterisation of Campylobacter from various sources [8–13].
In this study a robust and reproducible RAPD method was developed and used as a molecular typing tool to distinguish strains of Campylobacter from various sources and computer aided analysis employed to investigate strain relatedness.
2 Materials and methods
2.1 Bacterial isolates
Campylobacter isolates from environmental (n=21), poultry (n=17), clinical (n=43) and culture collection (n=5) sources were purified where necessary by culture on selective media (LabM, Amersham, UK) by standard techniques. Isolates were confirmed as Campylobacter by Gram-stain, colonial and microscopic morphology, motility, catalase and oxidase tests. C. jejuni and C. coli strains were not differentiated by hippurate hydrolysis as previous work (unpublished) with the described RAPD primer showed profiles not to be species-specific. In addition, hydrolysis cannot always be relied upon due to the existence of hippurate negative C. jejuni strains [14] suggesting that this test does not correlate with overall genomic diversity. Isolates were grown in 5 ml brain heart infusion (BHI) broth (Unipath, UK) with shaking at 200 rpm for 24 h in a 5% O2:10% CO2:85% N2 atmosphere. Bacterial DNA template was prepared from these cultures as described by Mazurier et al. [12] with modifications. Briefly, 3 ml of culture was centrifuged in a benchtop microfuge (5 min at 13 000×g), the culture supernate discarded and the cells resuspended in 1 ml PBS to wash. The cells were centrifuged again and resuspended in 500 ml H2O, placed in a boiling water bath for 10 min, centrifuged again and the supernate removed to a fresh tube. The supernate A260 was read and diluted to give a DNA concentration of 10 ng ml−1.
2.2 RAPD reaction
The PCR was performed in a 25 µl volume containing 2.5 µl 10× PCR buffer (100 mM Tris-HCl, 35 mM MgCl2, 250 mM KCl, pH 8.8), 0.5 µl 10 mM dNTPs (Promega, Madison, WI, USA), 0.6 µl 100 mM Primer (1254: 5′-CCGCAGCCAA-3′, [15]), 1.25 units of Taq DNA polymerase (Gibco-BRL), 19.15 µl H2O and 2 µl DNA template at 10 ng µl−1. Reactions were overlaid with mineral oil and amplified in a PTC-100/60 thermal cycler (MJ Research, Watertown, MA, USA) as follows: one cycle of 4.5 min at 94°C followed by 5 low stringency cycles of 30 s at 94°C, 2 min at 21°C, 2 min at 72°C, and 35 high stringency cycles of 30 s at 94°C, 1 min at 32°C and 2 min at 72°C. The cycling was concluded with 5 min at 72°C and the reaction products stored at 4°C prior to analysis.
2.3 Profile analysis
Amplification products were detected by agarose gel electrophoresis with ethidium bromide staining and recorded by the IS500 digital imaging system (Flowgen, Kent). Digitally captured RAPD profiles were analysed with GelCompar software (Applied Maths, Belgium) with the band matching coefficient of Dice and UPGMA clustering to determine profile relatedness. In addition, the discriminatory ability of the described RAPD reaction was determined from Simpson's numerical index of diversity [16].
3 Results
An example of the electrophoretic profiles generated by RAPD analysis of Campylobacter is shown in Fig. 1. The profiles were analysed in duplicate to highlight the reproducibility of the described RAPD protocol. Data from longitudinal studies also suggest that the optimised RAPD reaction can reproducibly recognise the same strain analysed years later (data not shown). The RAPD analysis resulted in 100% typability, all isolates generating a specific profile of DNA fragments which was unique among the isolates investigated. Simpson's numerical index of diversity was calculated as 0.999. Profiles from all the isolates are represented in digital form in the dendrogram in Fig. 2. In addition, the relationships between isolates on the basis of RAPD fragments can be determined using the dendrogram. The analysis showed indications of clustering at 50% similarity level of the chicken meat isolates in three separate clusters, A, F and G, and a separate large cluster (B) incorporating a majority of the chicken house isolates. Clinical isolates comprised in some cases well-defined and separate clusters (E) while others were scattered among the other isolates examined. Two small heterogeneous clusters, C and D, contained a mixture of environmental and clinical isolates, and cluster G a mixture of clinical and poultry isolates. The culture collection strains were also dispersed among the identified clusters, the majority of Penner isolates being grouped in B while Penner 36 was in the heterogeneous group G, and NCTC 11828 in group A.
4 Discussion
In contrast to the uncertainties of characterisation based on variable phenotypic characteristics, the application of molecular typing methods can provide a stable and highly discriminatory analysis of bacterial isolates. Random amplification of polymorphic DNA has been successfully applied to the differentiation of thermophilic campylobacters and is shown here to provide 100% typability of isolates from clinical, food and environmental sources. DNase activity, which can give rise to untypability of isolates of Campylobacter by RAPD [10] was not observed in this study. The diversity of profiles, also observed in RAPD studies of Campylobacter from marine sources [10], represents the heterogeneity of the genus which probably arises in part from the natural competency of, and horizontal gene transfer between, campylobacters [17]. This is also reflected in the diversity index of 0.999. This indicates that if two strains were sampled randomly from the Campylobacter population, on 99.9% of occasions they would fall into different RAPD types. This is not only a measure of the high discriminatory power of the described RAPD method but a further indication of the heterogeneity of the Campylobacter genus. An index of >0.9 has been suggested as desirable if the results of a typing scheme are to be interpreted with confidence [16], and the described RAPD protocol fulfils this requirement. Since all of the Campylobacter isolates generated a unique RAPD profile there existed the possibility that the typing scheme was excessively discriminatory. This concern was addressed by analysing isolates which, by detailed serotyping, biotyping and epidemiological data, had been identified as the same strain. When characterised by RAPD the profiles generated from these isolates were identical (data not shown) confirming that the described RAPD protocol could reliably and reproducibly recognise the same strain.
Several monomorphic bands were also present in a majority of the isolates investigated. These fragments have been cloned and sequenced characterised (data not shown), however, in contrast to Salmonella isolates [18] the current lack of published genomic sequence data makes possible functional attributes of these genomic regions difficult to ascertain. This situation is likely to change in light of current proposals to sequence the Campylobacter genome which will allow valuable additional information to be gained from RAPD profiles.
Seven distinct clusters were observed at the 50% similarity level encompassing the isolates investigated. Clinical isolates are represented in each cluster which suggests that it is not a sub-population of campylobacters which are responsible for human disease, but a wide variety of strains. These data may also suggest that poultry isolates are not as diverse, being represented by two distinct clusters, however more isolates should be investigated.
A significant advantage to having genetic profiles of isolates on a computer database is that retrospective studies can be performed to allow epidemiological inferences about the sources and routes of transmission of Campylobacter. Currently this information is rarely available, however, RAPD profiles represent stable genetic markers that may provide useful indications of Campylobacter epidemiology and allow subsequent control measures to reduce the burden of disease and associated costs to society.
This method appears to be a valuable tool for the classification of Campylobacter isolates. Excellent typability and reproducibility coupled with relative technical ease make RAPD suitable for the epidemiological investigation of Campylobacter. In the long term, as sequence information becomes available, RAPD profiles may provide useful additional information and by profile analysis possible molecular correlates of the origins of isolates may be found.
This work was funded by a grant provided jointly between the Faculty of Science, University of Birmingham and Campden and Chorleywood Food Research Association.
- © 1997 Federation of European Microbiological Societies.


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Kevin Estes: Arizona was one of Estes' earliest offers, but he now has 16 and is hearing from schools throughout the country. That has not slowed Arizona down and it appears that Estes could make a decision before the end of the summer.
"I'm still relatively wide open," said Estes. "Within the next month or two, I'd like to make my decision."
Estes admits BYU and Duke are coming after him the hardest, but does not say they are leading. In fact, Estes has been pretty quiet about that and it appears he could make a commitment without visiting first. Arizona is definitely in there, but with how Estes handles his recruitment, it is difficult to say just how much.
Cody Creason: Creason will be deciding in early August and Arizona is among his final four schools, along with Arizona State, California, and Utah. Arizona is the only school of that bunch that Creason has not visited, but he will not decide until he does make his way to Tucson.
"I've talked to their offensive line coach a couple of times and have heard great things about the school," Creason said. "It's the only school I haven't visited so I'll be there around the end of July. That's why I'm making my last trip there. I want to be able to see them. But I've heard nothing but great things about it."
It could be a good sign that Arizona made Creason's final four without even getting a visit. The coaching staff will have every opportunity to impress Creason to get a commitment and obviously the visit will go a long way with that.
Dru Samia: UCLA is among the schools really fighting for Sami and the Bruins could be tough to beat, but Arizona is working on trying to get Samia on campus should he decide to end his recruitment earlier than expected.
"I took a lot of trips and did some camps and it has been a tiring process," Samia said. "I'm thinking about moving up my commitment date but still need to sit down and talk things over with my family first."
Oklahoma and Texas are getting involved and the majority of the Pac-12 is on Samia, so it will not be easy."
Harper Sherman: We've said this before, but Arizona is likely the leader for Sherman. Nevada was involved early as well, but it would not be a surprise to see this recruitment come down to Arizona and Cal.
"I'm not quite sure about when I will commit," said Sherman. "I might make my decision earlier but I probably won't wait until December. I do want to go on some visits though."
Sherman has actually visited both places already, so a decision could seemingly come at any time. On that same note, his recruitment could also blow up when coaches realize just how good he is.
Nathan Eldridge: Arizona is the likely leader for Eldridge. He has played mostly defensive line, but impressed at Arizona's camp and earned an offer for the other side of the ball.
"I'm familiar with both and I really had a good time at Arizona," Eldridge said. "I got the offer and it was just a neat experience. They love my game and they seem to think that I can play anywhere on the interior line."
Eldridge wants to take his visits, but it would not be a surprise to see if Arizona tries to get a commitment out of him before that happens.


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Now this is just silly. The Washington Post can't be bothered to worry about Barack Obama's college years, college transcripts, communist friends, cocaine use, or cop-killing plotters in whose living room he first launched his major political career, but they can get in the really way back machine to 1965 and Mitt Romney's high school years.Mitt Romney cut a hippy's hair at his preparatory high school. A day after Barack Obama caved on gay marriage, the Washington Post "coincidentally" says Mitt Romney cut the hair of a boy who "was perpetually teased for his nonconformity and presumed homosexuality." Let's leave out the fact that the kid who got his haircut was subsequently thrown out of school for smoking one cigarette, but we're to believe that the assailants of his hair, witnessed by many, were ignored. Oh, and the guy who got is hair cut never, ever, ever mentioned it, including to family, and died in 2004 so it can't be verified. But a handful of students who now probably support Barack Obama have a crystal clear memory of events from 50 years ago. The people who were adults at the time of the incident and still alive have no memory of it, but remember Romney and said he was never a disciplinary problem.N*gg*rhead rock, anybody?After four years of ignoring Barack Obama's bullying of religious groups and others from inside the White House, it's fair game to go after Mitt Romney as a supposed high school bully.I guess we won't hear the left whining any more when we mention Barack Obama eating a dog since, as they are quick to point out, he was a kid when it happened.We have now had more in depth examination of Mitt Romney by the Gang of 500 than of Barack Obama.
We do not really know much about Barack Obama's constituent record in the Illinois Legislature.We still don't know his college transcripts.We still don't know what exactly he did for ACORN in his community organizing time.We still don't know all the details about the depth of the cocaine use Barack Obama admitted to in his book. Likewise, we know very little about his drug use in high school beyond just a few lines of reporting from 2007.We know very little, outside of his own autobiographical spin, about how he overcame his rage at white people — something he wrote about, but again, as a politician in an autobiography.We still don't know about just how many communists Barack Obama chose to surround himself with.We still know very little about Barack Obama's ties to Bill Ayers and the media sure hates to talk about Barack Obama getting his major political start in Bill Ayers' living room.But the press is perfectly happy to go back several decades to tell us every salacious detail about Mitt Romney the teenager.This reminds me of 1992 all over again. The George H. W. Bush team wanted to go focus on Bill Clinton the draft dodger from Vietnam because they couldn't fight on the economy. Unlike 1992, Barack Obama has the media doing it for him.


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Murrysville friends to run Boston Marathon, raise money for Franklin Regional victims
EXPORT, Pa. - Three friends from Murrysville will run in the Boston Marathon after a tragedy in their community last week.
Marlo Ayres, Jason Jelovich and Kim Pekarcik will wear Franklin Regional T-shirts and #FRStrong in support of the school where 21 students and a security guard were stabbed.
"God gave us a gift, and hopefully we can use our gift to raise some funds for victims of the Franklin Regional stabbings," said Ayres.
The marathon runners are looking for sponsors willing to donate $1 for every person who tracks their progress along the marathon course. That money would go to a fund.
"You can text 345678 our bib numbers. For every person that tracks us, the sponsor will donate $1 to the S&T fund," said Ayres.
See attached photo for bib numbers.
For Jelovich, this is deeply personal. He graduated from Franklin Regional High School and has two kids in the school system.
"It definitely has hit pretty close to home," he said.
Murrysville friends to run Boston Marathon, raise money for Franklin…
Fall might not be as colorful this year
Woman falls to her death in Go Ape zip line accident
Father grabbed alligator's snout to pry boy from reptile's mouth, report says
Consultant: I conspired with attorney general to frame aide


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The People of v. Travontae McKinley, KA 09-01307
- Appellate Division, Fourth Department
- Before: Smith, J.P., Peradotto, Lindley, Valentino, and Whalen, JJ.
- For Plaintiff: For Respondent: Susan C. Azzarelli of Counsel, William J. Fitzpatrick, District Attorney, Syracuse.
- For Defendant: For Defendant-Appellant: Piotr Banasiak of Counsel, Frank H. Hiscock Legal Aid Society, Syracuse.
Cite as: The People of v. Travontae McKinley, KA 09-01307, NYLJ 1202583127095, at *1 (App. Div., 4th, Decided December 28, 2012)
Before: Smith, J.P., Peradotto, Lindley, Valentino, and Whalen, JJ.
Decided: December 28, 2012
For Defendant-Appellant: Piotr Banasiak of Counsel, Frank H. Hiscock Legal Aid Society, Syracuse.
For Respondent: Susan C. Azzarelli of Counsel, William J. Fitzpatrick, District Attorney, Syracuse.
Appeal from a judgment of the Onondaga County Court (Anthony F. Aloi, J.), rendered June 18, 2009. The judgment convicted defendant, upon his plea of guilty, of criminal possession of a weapon in the second degree.
It is hereby ORDERED that the judgment so appealed from is unanimously affirmed.
Defendant appeals from a judgment convicting him upon a plea of guilty of criminal possession of a weapon in the second degree (Penal Law §265.03 [3]). Contrary to defendant's contention, County Court properly refused to suppress a handgun discarded by defendant while he was being pursued by a police officer, a controlled substance seized from his person following his arrest, and a postarrest showup identification. The officer who pursued defendant testified at the suppression hearing that the police received a 911 call reporting that shots had been fired near the intersection of East Fayette Street and Columbus Avenue in Syracuse. The information transmitted to the police indicated that four black males, at least one of whom was dressed in dark clothing, were reportedly involved in the incident. Within a minute and a half of the dispatch, the pursuing officer and two other police officers in an unmarked vehicle approached a group of four black males, one of whom was defendant, walking approximately one block from the scene of the alleged shooting. The police vehicle pulled up to the four individuals and, as the officers were exiting the vehicle, defendant and another male dressed in dark clothing fled in different directions. The officer who pursued defendant testified that he repeatedly yelled, "stop, police" while he was pursuing defendant. During the course of the pursuit, defendant discarded a handgun. The police thereafter apprehended defendant and, during a search incident to his arrest, discovered a bag containing cocaine. The witness who placed the 911
call then identified defendant as the shooter in a postarrest showup identification.
We conclude that, based upon defendant's physical and temporal proximity to the scene of the reported incident and the fact that the group of males matched the description of the individuals involved in the shooting, the officers had a founded suspicion that criminal activity was afoot, justifying their initial common-law inquiry of defendant (see People v. De Bour, 40 NY2d 210, 223; People v. Brown, 67 AD3d 1439, 1439-1440, lv denied 14 NY3d 798; People v. Williams, 39 AD3d 1269, 1270, lv denied 9 NY3d 871). We further conclude that defendant's flight as the officers began to exit their vehicle and his continued flight in defiance of orders to stop furnished the requisite reasonable suspicion to justify a greater level of police intrusion (see Williams, 39 AD3d at 1270), i.e., police pursuit (see People v. Pines, 99 NY2d 525, 526-527; People v. Sierra, 83 NY2d 928, 929).
Defendant contends that the police lacked reasonable suspicion justifying pursuit because the record does not establish that defendant knew that the approaching individuals were police officers, citing People v. Riddick (70 AD3d 1421, 1424, lv denied 14 NY3d 844). Even assuming, arguendo, that defendant's contention is preserved for our review, we conclude that it is without merit. Here, unlike in Riddick, the police were responding to a reported crime, and the police therefore had a founded suspicion that criminal activity was afoot before approaching defendant (see id. at 1422). Thus, under the circumstances of this case, including the report of shots fired and the fact that the four individuals matched the description of the individuals involved in the shooting incident, we conclude that defendant's flight from the officers and his refusal to stop after the officers explicitly identified themselves as police and he was directed to stop justified the pursuit of defendant (see generally People v. Bachiller, 93 AD3d 1196, 1197, lv dismissed 19 NY3d 861; Brown, 67 AD3d at 1439-1440; People v. Martinez, 59 AD3d 1071, 1072, lv denied 12 NY3d 856).
Because the record supports the determination of the suppression court that the police had reasonable suspicion to pursue defendant, defendant's abandonment of a handgun during the pursuit was not precipitated by illegal conduct and, thus, denial of suppression was proper (see Sierra, 83 NY2d at 930). The search of defendant's person, resulting in the seizure of the controlled substance sought to be suppressed, was incident to defendant's lawful arrest (see Williams, 39 AD3d at 1270), as was the postarrest identification of defendant.


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Top Definition
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بواسطة Anonymoos يوليو/تمُّوز 24, 2005
moving downward without controll; usually landing face down.
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many; much (West Coast, esp. Pacific NW, urban)
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بواسطة still swass مايو/أيار 3, 2005


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Apr 14, 2013; New York, NY, USA; American rap artist Drake at the game between the New York Knicks and the Indiana Pacers at Madison Square Garden. Knicks won 90-80. Mandatory Credit: Anthony Gruppuso-USA TODAY Sports
NCAAF 2hr ago
South Carolina TE tried to hurdle Kentucky defender, got slammed to the ground instead
That looks like it hurt.
NCAAF 3hr ago
Photographer says she's OK after getting drilled in the face by Auburn kickoff
She was trying to catch it.
NCAAF 4hr ago
Penn State's 258-pound kicker leveled another returner
NCAAF 5hr ago
15 of the best tweets about Duke's stunning win over Notre Dame
Twitter loves a good upset.
High School 6hr ago
High school boys soccer team forfeits because 2 girls were on the other team
The team cited religious reasons.
MLB 8hr ago
Mookie Betts celebrated Red Sox win by doing 'The Carlton,' asks fans what dance he should do next
What should he do next?