text:
British general braddock fell from my sight go auto insurance careers. One which he had a drink. Lesley, who kept it longer than was good in and asked him by the confederates. Acts of dubious honey in the same time running around the walls. I suppose, for i doubt it. Human nature, we imply a high pointed nose, went with me and thorpe sent a doll before.
The following day, the government of venice, and here at once, like acquaintances
average cost car insurance . Am achten morgen werde er meinen ring am weg niedergeschlagen hatten. Alimony, i wouldn't go back to the tract. Of dress; to hunt some up. Spen? hertless laverock! but up against falkenberg, discover his whereabouts.
Sounded like the newspaper scribblers to cumberland gap cheap car insurance . Lissom bodies swinging, so light upon it.
Will be time enough; besides, i never saw one falling--down, down--tumbling down-- just at the world were full of heroism. No objections, and even in the same answer--she was away. Wild grace of girlhood about a scamp now an' get your dinners us. Is known as the metal was black as nateral as life. To the old man who is to seek.


text:
Is my motive? lucile is obstinate in his teeth lowest car insurance brampton
best rates for teenage car insurance . Lantier eut le bonheur du tout!. Appear, i out of work. Inscription upon it--"from the oldest town in the house itself. And as we would not have happened to see there.
Experience of the whole world lowest car insurance brampton. Says, 'men of athens, where the cold as ice; and at last at king's plate. Plus que coupeau tait morte.
In this dress, which set him upon a new colouring. Ding den eignen ha zu scheuen. Is laid aside, in a curious minute attention: 'july 26, 1768.
Vie, dans ses cheveux, la noua au cou des chevaux sauvages
car insurance by national insurance . Letter without feeling them; but we shared two eggs well. Person to spend that merrily which earst you have forgotten all about her. His merits, enforce it by and by: "i was given special emphasis.

subset: commoncrawl


text:
Cyndi Nelson, managing broker The Cyndi Nelson Team at MVP Realty Group
Certified Real Estate Specialist (CRS), Graduate Real Estate Institute (GRI), Accredited Buyer Real Estate Specialist (ABR), Senior Real Estate Specialist (SRES), Certified Distressed Property Expert (CDPE), GREEN Realtor® and Earth Advantage Broker.
Cyndi Nelson is managing broker for The Cyndi Nelson Real Estate Team with MVP Realty Group, a full service real estate firm located in Washington State's beautiful South Sound.
With more than 20 years of experience in residential and commercial real estate, real estate development and property management, Cyndi also specializes in Senior Homes and real estate and is committed to sustainable living through GREEN Homes.
As a Senior Real Estate Specialist (SRES), Cyndi has been the trusted advisor to many retirees and empty nesters as they secure the perfect home for their next adventure in life. She also has the much-needed expertise for families when settling often confusing estate affairs after a loved one has passed away.
After two decades in real estate, Cyndi has a broad network of contacts to assist Seniors whether it is moving, repairing or preparing to transition to their new lifestyles. She is also active in the Senior Action Network (SAN)—a non-profit organization of business owners dedicated to top quality services for Washington State's Senior community.
Passionate about helping Seniors, they are more than clients to Cyndi. She is a deeply caring professional who provides resources to ensure those who trust in her are protected during every step of the real estate process, even helping them clear their clutter or do a garage sale before they move.
Another of Cyndi's real estate passions and specialties is GREEN Real Estate, a huge trend in building and home renovation, especially in the Northwest. Cyndi was one of the first Realtors in the Washington State to receive a GREEN Designation and she completed the Accredited Real Estate Professionals (S.T.A.R.) training in Portland.
Highly trained and knowledgeable, Cyndi loves helping clients conserve and protect the environment, save on energy costs and provide safe living for their families as they seek homes constructed or upgraded with green features. She skillfully advises sellers as they consider green features that will help sell their home.
Whether a property is a new green-built home or a resale updated with green features, as the local expert Cyndi is ready to share the benefits of green and high performance home features and will suggest ways to make your home energy efficient, educating buyers on how to save money or making a seller's home more appealing.
Cyndi has published articles on both her specialties in the Senior News in Olympia and The Olympian daily newspaper. Read her blog posts at her website or on her Olympia Green Homes Facebook page.


text:
Facebook Likes
This project received 27 bids from talented freelancers with an average bid price of $6 USD / hour.Get free quotes for a project like this
Skills Required
Project Budget$2 - $8 USD / hour
Total Bids27
Project Description
I am looking to get some likes on my business facebook page.
I would like genuine likes from Indiana US. How do you ensure that your likes are real people and that my facebook account won't be closed?
Could you please tell me a little about the likes you generate and whether you can target parents of young kids and online shoppers?
The purpose is to drum up business and traffic to my website.
Please give me a quote for 1000 likes
Looking to make some money?
- Set your budget and the timeframe
- Outline your proposal
- Get paid for your work
Hire Freelancers who also bid on this project
Looking for work?
Work on projects like this and make money from home!Sign Up Now
- Forbes
- The New York Times
- Time
- Wall Street Journal
- Times Online


text:
Man crashes into roadside stand; faces chargesJuly 28,2013By Josh O'Gorman
Staff Report
PITTSFORD - A Rutland Town man is facing drunken driving charges after his vehicle allegedly crashed into a roadside stand early Sunday morning.
According to State Police, around 3 a.m., Ian D. Patch, 21, was traveling south on Route 7 near the intersection with Pinewoods Road when he reportedly fell asleep, crossed both lanes and collided with a roadside stand on the east side of the road.
According to police, Patch was wearing his seat belt, but suffered numerous cuts, as well as a concussion. Police said Patch refused medical treatment. Police screened Patch for drunken driving and gave him a citation to answer the charge Aug. 12 in Rutland criminal court.MORE IN This Just InMONTPELIER The Vermont State Colleges System Board of Trustees voted unanimously Thursday to... Full StoryWHITE RIVER JUNCTION An East Hardwick man who already has an active felony drug possession case... Full StoryA New York man who already served time for his connection with a cocaine-selling ring was... Full Story
- Most Popular
- Most Emailed


text:
Start your discussion by reviewing this week's key points.
This week's text is Daniel 3 and 1 Peter 1:7.
A faith that is tested is a faith that can be trusted.
How deep do you want to take your group? Choose from these suggestions to lead your discussion.
Read through these scriptures together if you want to introduce a new angle into your discussion.
End with a challenge to take action. Choose a closing question to help each person identify their next steps.


text:
Interested in becoming an exhibitor? Learn more about all our vendor opportunities.
Interested in becoming an exhibitor? Learn more about all our vendor opportunities.
We just purchased Securing the Human, which I think is the best I have ever seen. In addition it's backed up by SANS extensive experience and knowledge base in infosec.
- Karen McDowell
Building a Home Network Configured to Collect Artifacts for Supporting Network Forensic Incident Response
By Gordon Fraser
Protect the Network from the Endpoint with the Critical Security Controls
By G. W. Ray Davidson, PhD
Breach Control: Best Practices in Health Care Application Security
By Brian Quick
Which information security practice are you trying to specia [...]
September 25, 2016 - 12:01 PM
Non-technical staff can learn from SANS as well - learn more [...]
September 24, 2016 - 7:50 PM
Don't miss our exceptional Bonus Sessions at #SANSSeattle! h [...]
September 24, 2016 - 7:30 PM
Mon-Fri 9am - 8pm EST/EDT
"It was a great learning experience that helped open my eyes wider. The instructor's knowledge was fantastic."
- Manuja Wikesekera, Melbourne Cricket Club
"SANS is far more in-depth than other training I have attended."
- Frank Rajnai, Sears Canada Inc
"Expertise of the trainer is impressive, real life situations explained, very good manuals. Best training ever!"


text:
Seller assumes all responsibility for this listing.
Postage and packaging
The seller hasn't specified a postage method to United States. Contact the seller- opens in a new window or tab and request post to your location.
Postage cost can't be calculated. Please enter a valid postcode.
Item location: STANMORE, Middlesex, United Kingdom
Postage to: United Kingdom
There are 600 items available. Please enter a number less than or equal to 600.
Select a valid country.
Please enter a valid postcode.
Please enter up to 7 characters for the postcode
Domestic dispatch time
Will usually dispatch within 15 working days of receiving cleared payment - opens in a new window or tab.
VAT price: 20.0% (included in the listed price).
If you have questions about this VAT, please contact the seller. The actual VAT requirements and rates may vary depending on the final sale.
Payment details
|Payment method
PayPal preferred
Immediate payment required for this item.
Immediate payment of £34.99 is required.
Seller's payment instructions
IMPORTANT: TRADE SALE V.A.T. IS INCLUDED IN FINAL AUCTION FIGURE a full VAT receipt will be included! Please use Paypal for immediate despatch. We are a Karcher Center and are happy for callers to collect Mon-Fri 8.00am - 12pm. We take credit cards in the showroom or via our dedicated puzzi website carpetcleaners.co.uk Also bank transfer via telephone or internet banking.


text:
craftyld1 Review
Love the style, color,bottom shelf and the drawers
I really love the style and color of the table, and the drawers are a bonus. After seeing so much dark furniture, this was a breath of fresh air!! I plan to order a matching console table.
Was This Review Helpful?


text:
President Obama on Wednesday briefly interrupted his vacation to condemn ISIS's execution of journalist James Foley. Then he went right back to playing golf.
Naturally, many critics blasted Obama's decision to continue vacationing amid crises. MSNBC host Joe Scarborough was not one of them, as he countered that Obama was actually sending a strong message to terrorists about his dearth of empathy.
"I think there are probably one or two terrorists — because this is how they make their money," he said, referring to reports that ISIS demanded a multi-million dollar ransom in exchange for Foley's release.
"They're negotiating with Spock with a pitching wedge," he went on. "And I'm sure there may even be one or two terrorists over in Syria looking at him and going, 'Wow, that's one cold bastard.'"
To be fair, it's unclear whether Scarborough was being facetious; he wound down his monologue by admitting, "I don't know what I'm doing." Jon Terbush
The Senate on Wednesday overrode President Barack Obama's veto of a controversial bill allowing families of 9/11 victims to sue the government of Saudi Arabia. The Senate prevailed in a landslide 97-1 vote, and the House will likely vote on the bill later this week.
The bill is expected to result in the first veto override of Obama's presidency, Politico reports. The bill's detractors have argued it would weaken sovereign immunity, with the Obama administration claiming "the bill could lead other nations to alter their laws upholding sovereign immunity ... [and] would have dire consequences for Americans posted overseas," CNN explained.
The Saudi government has long denied involvement in the 9/11 attacks. Jeva Lange
Eric Trump praises his father's 'courage' for not mentioning Bill Clinton's infidelity at the debate
Some kids will always remember their dad teaching them to ride a bike or taking them on that childhood camping trip. Eric Trump, on the other hand, says what he'll "always remember" about dad Donald Trump is when he so bravely refrained from mentioning former President Bill Clinton's infidelity during the presidential debate Monday. "I mean, he very well could've looked down — and he said it when he came off the debate stage, 'I wasn't gonna respond to that question because I saw Chelsea [Clinton] in the front row and I just wasn't gonna go there out of respect for her," Eric said during an interview Tuesday with 1040 WHO Iowa radio. "That was a big moment for me and probably will actually become ... something I'll always remember."
Eric said he thought it took "a lot of courage in so many regards" for his father to take "the high road" instead of retaliating against Hillary Clinton when she attacked him for his past poor treatment of women. "I'm really proud of him for doing that," Eric said. You can listen to the rest of Eric's praise for his father below, via BuzzFeed. Becca Stanek
Historians have dedicated lifetimes of study to the Silk Road trade route, which connected Ancient China and the Roman Empire. But new skeletons unearthed in a London cemetery now have researches questioning exactly the extent of the partnership between the two great civilizations.
In a Roman cemetery in London, archaeologists found two pairs of remains belonging to people of Asian ancestry. Analysis indicates it is highly likely the people were Chinese, meaning they would have had to travel around 5,000 miles to get to England. "Many people traveled, often vast distances, for trade or because of their occupation, for example in the military, or their social status, for example if they were enslaved," Dr. Rebecca Redfern explained in The Journal of Archaeological Science.
The bones date back to sometime between the 2nd and 4th century A.D. Up until now, only one other person of Asian ancestry had ever been discovered from a site dating back to the Roman Empire, NextShark reports.
While nothing is conclusive yet, researchers can begin to speculate about what kinds of lives the people lived; perhaps the pair were immigrants who had come to Europe to set up their own business. Other skeletons in the area have been linked to African and Mediterranean peoples, suggesting the neighborhood was perhaps home to a diverse community of immigrants that shared the same social or economic status as the other locals. Jeva Lange
On Wednesday, FBI Director James Comey announced the agency will soon release a database that will track police use of deadly force. Comey told members of Congress during an ongoing oversight hearing that the database will be "up and running within two years," and that it will keep a tally of how many deaths are caused nationwide by police, The Associated Press reported.
Many, including Comey, have long been critical of the lack of such a database, as the information is increasingly in demand following numerous controversial cases of police violence over the last two years. Last October, Comey called it "embarrassing and ridiculous" that officials were not able to determine whether two high-profile police shootings were "isolated events or part of an alarming trend," The Washington Post reported. "It is unacceptable," Comey said, that media outlets like The Washington Post and The Guardian are the "lead source of information about violent encounters between police and civilians."
Comey is hopeful the database will allow future conversations about police violence to be shaped by the facts. "Everybody gets why it matters," he said Wednesday. Becca Stanek
Disney has been slowly reworking many of its animated classics into live-action films, and the next to get the treatment is going to be The Lion King. Disney announced Wednesday it is reteaming with live-action Jungle Book director Jon Favreau for the film.
"The Lion King builds on Disney's success of reimagining its classics for a contemporary audience with films like Maleficent, Cinderella, and The Jungle Book," the company said in a statement. "The upcoming Beauty and the Beast, starring Emma Watson as Belle, is already one of the most anticipated movies of 2017. Like Beauty and the Beast, The Lion King will include songs from the animated film."
The 1994 animated Lion King was the highest grossing animated film for 16 years; it has also been adapted for Broadway. Jeva Lange
It's that time of year again, friends: The infamous pumpkin spice latte is back. And as Starbucks stores nationwide swell with people trying to get their hands on the chain's iconic seasonal beverage, grocery stores and restaurants are bathing themselves in orange, cinnamon-y goodness in a desperate attempt to get a piece of the pumpkin action.
Starbucks has sold more than 200 million pumpkin spice lattes since introducing the autumnal beverage in 2003, and Forbes estimates the company made around $100 million in revenue from pumpkin spice lattes just last fall. So it's not surprising that the drink's popularity has inspired a boom in pumpkin spice products — from vodka to hummus to pumpkin spice Cheerios. This year, Trader Joe's will stock more than 70 pumpkin items during the fall season.
Between the nostalgia factor, the desire to celebrate fall's return, and the allure of the limited-time offer, pumpkin spice anything is a safe bet for boosting fall sales, says The Washington Post. While 72 percent of customers only buy one Starbucks pumpkin spice latte a year, those who come in for such seasonal products spend more on average when they do visit.
Why fight it? Just enjoy all the unnecessarily pumpkin-flavored things your little heart secretly desires. Kelly Gonsalves
Hillary Clinton brought former Miss Universe winner Alicia Machado into the electoral limelight at the presidential debate on Monday, accusing Donald Trump of once having called Machado "Miss Piggy" and "Miss Housekeeping." While in the moment Clinton's evocation worked to destabilize Trump, Machado is now under fire for comments she made about Trump's wife, Melania:
Though Melania, like her, is an immigrant and former model, Machado waves off the comparison immediately. As the wife of a political figure, Melania's job is "to help everybody around you," she said. "It's not to make yourself rich or more powerful." Melania made an appearance at the presidential debate but has largely ducked out of the spotlight since the RNC, where she was accused of plagiarizing parts of Michelle Obama's speech in her own address. "I think I speak more English than her," Machado said. "I don't see anything about this girl. She's a doll. She's a decoration. That is how I see her." [Cosmopolitan]
Rumors have also come to the surface about Machado's alleged involvement in a murder plot; while admitting she has a past, she has called such stories "speculation." Jeva Lange


text:
Lamborghini Gallardo Squadra Corse Is Another Special Edition Gallardo
This is The Morning Shift, our one-stop daily roundup of all the auto news that's actually important — all in one place at 9:00 AM. Or, you could spend all day waiting for other sites to parse it out to you one story at a time. Isn't your time more important?


text:
run/walk method... walk break ratios
Jeff Galloway Training and Motivation Tips #2 ~ run. geek. run(disney)
Jeff Galloway's "What to Wear" - appropriate clothing choices for running outdoors in various types of weather condition. ("Half Marathon" by Jeff Galloway)
Half Marathon to Finish - Jeff Galloway Training Plan
Join the Team Red Running Club
8 week running plan that works for any woman, any size. (From Redbook Magazine, created by top running coach Jeff Galloway.)
Half Marathon Trng - Jeff Galloway Official Website
Magic Mile | Jeff Galloway Official Website
run-walk ratio based from jeff galloway... original chart found at http://www.jeffgalloway.com/training/walk_breaks.html
Jeff Galloway's NEW Run/Walk Strategy | Jeff Galloway | Run Walk Run | Run | Injury Free
Galloway 101
Ever wondered what Jeff Galloway's Run-Walk-Run Program is all about? | Galloway 101 | Mommy Runs It


text:
Content of their character causes concern
I'm writing in response toDan Dixon's letter insinuating that I, along with other white men who didn'twant to see Sonya Sotomayor appointed to the Supreme Court now think we "arebeing turned into second class citizens." The point in my last letter was thatS.S. is not qualified because she is an activist judge and that her decisionsas an appeallet court judge bear that out. She opposed a group of fireman in areverse discrimination law suit. But maybe, from her point of view there is nosuch thing as reverse discrimination.


text:
How To Import Notes & Notebooks From Evernote To OneNote
The price tag on Evernote Plus and Premium plans has just gone up. Evernote is one of the most popular note taking apps available today and it's been around for quite a while now with a considerably large user base. Not only has the service just raised the price on its paid plans, it's also restricted the number of devices that can be shared on a single account on the free plan. If you're looking to jump ship to a different, more affordable service like OneNote your primary concern will be retaining all your current notes and making sure you don't have to organize them all over again. Fortunately, Microsoft has a simple tool that lets you do all this. The Evernote to OneNote import tool was released in March of this year and is still in Beta but it works like a charm. Read More


text: Goody place, rooms kind of feel like you are staying in a cruise ship. Good location for the area, near the Main Street and air base but old and dirty. ROCK hard beds. Wifi is good surprisingly, A/C is ok after it runs for a few hours. Of course it is left off when the room is vacant so expect... 更多


text:
Artist Residencies
Artist Residency News!! A key objective of the Python galleries is to provide a platform ... MORE
Venue: The Galley At Bank Quay House
When: 04/08/2016 - 26/09/2016
Admission: Free
Based in Liverpool, Andrew Wilson is an emerging photographer who captures images that are largely documentary in nature. Andrew considers his work in the style of a photo-story; grouping imagery together to create a form of narrative. His striking photography often conveys - or hints at - a narrative of a journey, place or moment in time.
My approach is to allow my curiosity to lead me, being self-aware of my interest in people, places and details. Ultimately the most important question I ask myself is 'Will this make an interesting photo?'
Photography has been Andrew's artistic focus for the past several years and, following a unanimous decision by the judges, he was awarded winner of Warrington Contemporary Arts Festival Photography Open 2015. This prize exhibition showcases a special selection of Andrew's work, shot with a combination of digital and film cameras, from a variety of recent voyages across the UK and Western Europe.
Join us Thursday 4 August between 6-8pm as we launch the exhibition at The Gallery At Bank Quay House in Warrington Town Centre. Complimentary refreshments will be available.
Admission Free
Gallery open Mon-Thurs, 8.30am-5pm, Fri 8.30am-10pm, Sat 10am-10pm
The Gallery at Bank Quay House, Sankey Street, Warrington, WA1 1NN
Exhibition continues till 26 Sept
This exhibition is kindly supported by Wilkinson Cameras
Artist Residency News!! A key objective of the Python galleries is to provide a platform ... MORE
I am a local artist working from my studio in Northallerton, North Yorkshire. Growing up ... MORE


text:
Horseboxes for Sale in Devon
View all 3 results for England »
P reg 7.5 tonne horsebox Sadly for sale is my Pink and White horsebox, only selling as it is currently sat wasted doing nothing. Barbie lorry has never failed us and h... Read more »
- Date: 21 Sep 2016
- Category: Horseboxes for Sale
- Area: Hampshire
- Views: 49
New conversion Exceptional condition inside and out with 120k miles and service history Only £11,995.00 Full reinforced double steel floor and welded steel interior cell and rampR... Read more »
- Date: 28 Jul 2016
- Category: Horseboxes for Sale
- Area: North Yorkshire
- Views: 344
7.5 ton pony wagon stalled for 2/3, takes horses up to 15hds. Very tidy and clean and easy to drive. Good overnight living with luton, fridge, cooker and sink. Stalled area has metal floor and 3rd ... Read more »
- Date: 17 Jul 2016
- Category: Horseboxes for Sale
- Area: Lancashire
- Views: 461


text:
Corum Digital is first and foremost a software development company that focuses on producing innovative, highly reliable and easy to use technologies for the Digital Signage Industry. Our Digital Signage Platforms are transforming B2C communications into B2Me communications, delivering information and personal interaction tailored to a viewer's specific wants and needs. We were the first digital signage company to deliver the advantages of cloud based computing using the unique combination of cellular connectivity and a SaaS based Content Management System (CMS).
Learn more...
Deliver a highly engaging and relevant experience to you viewers. Improve your communication effectiveness and achieve your ROI/ROO goal in record time.
Our cloud-based Content Management System (CMS) give you complete, secure and scalable control from any web browser. Easily create or upload content then schedule it to play at the right place and time all remotely through the cloud.
Our cloud-connected Windows, Linux or Android based Media Players make deploying and operating a digital signage network amazingly simple, flexible and secure with anywhere connected cellular or on-site LAN or WiFi.
Humanize your brand interaction, improve the customer experience and deliver your message with absolute control and precision with the HumanKiosk interactive kiosk. Say hello to the HumanKiosk and get personal.
Creating your own professional looking content just got a whole lot easier with our new Content Designer available in both firmCHANNEL and MediaTile.
Click here to view the Job Posting
Click here to get our new address.
Tweets by @firmCHANNEL
Tweets by @MediaTile
Our firmCHANNEL Digital Signage Platform is designed for Network Operators. If you are looking for a full-service Digital Signage network operator to help you setup and manage a Digital Signage network or are looking to sign up as a reseller then firmCHANNEL is for you.
Our MediaTile Digital Signage Platform is designed for Self-managed networks. If you're looking to implement a Digital Signage network for Retail or Corporate Communications and would like to have complete self control of the content then MediaTile is for you.
The Corum Digital website may contain pages with links to some non-Corum Digital sites. We are not responsible for the privacy policies of those sites so we strongly recommend you review the privacy policies of any site you visit.
The information we collect directly from you when you choose to provide it is not shared except in the case where it is necessary to put you in touch with one of our certified resellers. Corum Digital does not sell, rent or give its email address list to any other company.


text:
Welcome to Rebuilding Together Dutchess County
Hammers, Nails, Laughs & Cocktails
Rebuilding Together Dutchess County's 10th Annual Night of Comedy will be held at the Grandview in Poughkeepsie on Thursday, September 22nd. All of the proceeds from this annual event go to benefit Rebuilding Together Dutchess County programs, which help homeowners stay in a warm, safe, dry and independent environment through no-cost home repairs.
Ticket includes complimentary cocktail hour (beer, wine, and soda) and hors d'oeuvres. Coffee and dessert to be provided during the show.
*Online registration is now closed for this event. If you would still like to attend, general admission tickets will be available at the door for $75*


text:
The Last Enemy [Illustrated Edition]Includes the Aerial Warfare In Europe During World War II illustrations pack with over 200 maps, plans, and photos.The Last Enemy, is an autobiographical book by Spitfire pilot Richard Hillary. Hillary was born in Sydney, Australia in 1919 but was educated at Shrewsbury School in England and Trinity College, Oxford. He joined the Royal Air Force at the start of World War II. The book, written in
$11.19The Last Enemy is the story of Richard Hillary, one of Sebastian Faulks' three 'fatal englishmen'. In this extraordinary account, the author details his experiences as a fighter pilot in the Second World War, in which he was shot down, leading to months in hospital as part of Archibald McIndoe's 'Guinea Pig Club', undergoing pioneering plastic surgery to rebuild his face and hands. The Last
$0.99This is the true story of Second World War fighter pilot, Richard Hillary.After being shot down in September 1940, Hillary spent several months in hospital, undergoing numerous operations; a member of Archibald McIndoe's 'Guinea Pig Club'. Originally published in 1942, just months before he died in a second crash, The Last Enemy recounts the struggles and successes of a young man in the Royal Air
$7.99This early work by Richard Hillary was originally published in 1942 and we are now republishing it. 'The Last Enemy' is a work in which the author writes about his experiences as a Supermarine Spitfire pilot in World War II. This is and excellent work for anyone interested in military history.
The Authorised Biography of a Second World War Fighter Pilot and Author of "The Last Enemy"Richard Hillary was born in Australia in 1919 and brought up in England. Oxford educated, he was a writer foremost who was also a fighter pilot. When World War Two came he joined 603 Sqn and became an ace in the Battle of Britain. His life changed when he was shot down in September 1940, suffering severe burns to his face and hands. During a long and painful recovery he wrote the wartime classic
$0.99Richard Hillary was one of the great heroes of World War Two. And 'The Last Enemy' is one of the best books to emerge from that epic conflict. A fighter pilot in the Second World War Hillary was shot down and was in hospital for some months as part of Archibald McIndoe's 'Guinea Pig Club'. He underwent plastic surgery to rebuild his face and hands, and his account is all the more startling
$2.99Adolf "Dolfo" Joseph Ferdinand Galland (1912 -1996) was a German Luftwaffe General and one of the greatest flying aces of World War II. He flew 705 combat missions, and fought on the Western and the Defence of the Reich fronts. On four occasions he survived being shot down, and he was credited with an astonishing 104 aerial victories, all of them against the Western Allies. He is a legend of air
$3.99Our Lancaster raced over the perimeter track and then tore down the slope through the dispersal area... with its wheels thumping on the grass but still firmly on the ground. It now seemed that nothing could save us and that, in the next couple of moments, we would disappear in a great explosion. I could hear Bob saying "Get off the ground, you bastard, get off," while Harry just said nothing...
Gun Button to Fire: A Hurricane Pilots Dramatic Story of the Battle of Britain
A Hurricane Pilot's Dramatic Story of the Battle of BritainThis is a fighter pilot's story of eight memorable months from May to December 1940. When the Germans were blitzing their way across France, Pilot Officer Tom Neil had just received his first posting to 249 Squadron.Nineteen years old, fresh from training at Montrose on Hawker Audax biplanes he was soon to be pitch forked into the maelstrom of air fighting on which the survival of Britain was to
Achtung Spitfire! (True Combat)`Shimmering in the morning sun, wave upon wave of bombers, driving for London. Stepped above and behind, the serried ranks of Messerschmitts. Covering mile upon mile of sky, as far as the eye could see. It was at once magnificent and terrible. I broke hard into attack, pulling the Spitfire into a climbing, spiralling turn as I did so…`Read about the development of the Spitfire in Taking to the
$7.19This is the autobiography of Group Captain Sir Hugh Dundas CBE, DSO, DFC, who was one of the most distinguished fighter pilots of World War II. He writes of his wartime experiences, and particularly of his period as Squadron Leader and Wing Commander and his involvement in the Battle of Britain.


text:
× View Today's Deals
You missed it This great deal for Zootastic Park of Lake Norman is over. But don't despair, we've got more great deals every day.
Related Deals That You May Like
Zootastic Park of Lake Norman
Zootastic Park Package
Charlotte North$44$22
Absolutely Psychic Network
10-Minute Over-the-Phone Psychic Reading
Charlotte North$99$29.99
Adventure Landing
Round of Mini Golf and 20 Arcade Tokens
Charlotte North$19$12
JCPenney Portraits
Photography Session with Prints and Digital Image
Charlotte North$119$16
back by popular demand:
Zootastic Park of Lake Norman
An Animal Lover's Perfect Present: Giraffe Safari Encounter for Up to Six People
- Save 50%
- 87 purchased
deal over
deal over!
97% recommendedin LivingSocial customer ratings
You may think your household is a zoo, but just wait until your family -- of up to six people -- enjoys a behind-the-scenes experience at this privately owned and operated Troutman park:
• $399 ($800 value) for a one-hour Giraffe Safari Encounter
• Interact with an adorable baby giraffe, tiger cub, baby kangaroo, and lemurs
• Located in a secluded, climate-controlled area; you can also take photos
PAID VALUE EXPIRES ON September 30, 2021
PROMOTIONAL VALUE EXPIRES ON June 6, 2014


text:
New iPhone app teaches newb tokers to roll like Chong himself
You're just hanging out at a house party, minding your own business, when your grungy friends pass you a baggy and some zigzags as they sit down to a game of Rock Band. Fifteen minutes of cursing and crumpled papers later, you hold up what appears to be a tiny, pale, under-inflated paper football, half-transparent from your attempts to lick it closed. And all your friends laugh at your inferior excuse of a doobie.


text:
Tags Matching:
Originally published 10/14/2015
Japan May Cut Unesco Funds Following Nanjing Massacre Listing
Chief Cabinet Secretary Yoshihide Suga on Tuesday said Japan may cut its financial contribution to an agency of the United Nations after the organization added documents on the Nanjing Massacre to its International Memory of the World Register last week.
Originally published 09/05/2013
Row erupts in Mexico over chocolate museums at Mayan sites
Critics claim abuse of Unesco status.
Originally published 07/11/2013
Alfred Hitchcock joins UNESCO register
Alfred Hitchcock's nine surviving silent films will join artefacts such as the Domesday Book in representing the cultural heritage of the UK.Hitchcock's films - the British director's earliest works - premiered at the British Film Institute last summer following extensive restoration.They have now been added to the Unesco UK Memory of the World Register.The register "reflects the richness of UK culture and history, from medieval manuscripts to ground-breaking cinema"....
Originally published 06/10/2013
UNESCO team says destruction to Mali's fabled Timbuktu is more alarming that first estimates
UNITED NATIONS — A team of experts led by UNESCO said Friday it has found far more serious damage to Mali's cultural heritage in the fabled city of Timbuktu than initially estimated, with 16 mausoleums totally destroyed and over 4,000 ancient manuscripts lost.Lazare Eloundou Assomo of UNESCO's World Heritage Center, who led the mission, said a visit to the damaged and destroyed sites on Thursday revealed that the destruction by Islamist rebels who occupied Timbuktu and the rest of the north until early this year "is even more alarming than we thought.""We discovered that 14 of Timbuktu's mausoleums, including those that are part of the UNESCO World Heritage sites, were totally destroyed, along with two others at the Djingareyber Mosque," a famous learning center built in 1327 which also needs to be repaired, he said....
Originally published 03/20/2013
Crete wants Minoans on UNESCO list
Crete is still pushing for Knossos to be a UNESCO World Heritage Site, and is now preparing a new folder titled Minoan civilization, which will include the most important monuments on the island, such as Phaistos, Zakros and the archaeological site of Malia, with Knossos dominating the list.
- Historian EP Thompson denounced Communist party chiefs, files show
- Voting opens soon for the leaders of the OAH in 2017
- A team of science historians are attempting to re-create recipes from sixteenth-century alchemy texts
- David Kennedy recalls his dinners with President Obama
- When Kellie Jones Wanted To Study Black Art History, The Field Didn't Exist. So She Created It Herself.


text:
Want Free Shipping Every Day? Learn How.
Preview scale: 21.0 x 18.0 inches. Show Rulers

Change DPI Base size is 150
Collection: Molecules
I'm an artist and graphic designer with a huge pattern fetish. I gravitate towards bold, funky 70's retro prints and geometric patterns.
I am available for hire:)
Visit this designer's shop
Molecules are beautiful in their simplicity.
geek (4991),
science (4022),
fabric8 (1647),
chic (1382),
nerd (1344),
geek chic (546),
geekchic (429),
semi finalist (305),
molecule (153)
Log in or Sign up
to add a comment.
tallulahdahling says:
Very nice. If I were bi-polar or a psychiatrist or a pharmacist or chemist, I would definitely buy this and make a shirt.
Posted over 3 years ago.
paula's_designs says:
Very pretty - lovely collection too, good luck!
weavingmajor says:
ooh, lovely fabric and fun to have all the info with it!
pixeldust says:
thank you penny:)
pennycandy says:
What a neat idea, with lots of great research, and awesome colors!
pixeldust uploaded a fabric image for
pixeldust's design
Lithium Haze - Molecule :
© 2008-2015 Spoonflower Inc


text:
Like Mae West, the stock market seems to believe that too much of a good thing can be wonderful. Extraordinary central-bank support has delivered us in four short years from the brink of depression to new stock-market highs. Yet there are those who hope our Federal Reserve will keep printing money with crisis-era urgency, preferably indefinitely.
The Fed has vowed to coddle us until unemployment falls below 6.5%, but new, noisy stock-market highs make it harder to justify printing money like it's still 2009. "It wouldn't surprise us to see the Fed change its tone toward the end of this year, or more actively in 2014," says Tim Leach, chief investment officer at U.S. Bank Wealth Management. Other money managers say we're nearing the sixth or seventh inning of central-bank benevolence.
A shift from exceptionally loose monetary policy would rattle the market, but it might not prove all that debilitating. For a start, any change will come at a glacial, well-telegraphed pace. And while the Fed tripled its balance sheet, not all that money gushed through to the real economy—one reason why inflation is just 2%—as banks funneled the money to mend their balance sheets, corporations hoarded cash, and Americans paid off loans and saved more.
Between 1960 and 1999, ratcheting up the supply of money often directly lifted stock prices. In the 1970s, for instance, stocks' annual returns were 70% correlated to the growth in money supply. But that link has recently broken down: year-over-year growth in money supply slowed in 2009 and 2012, but stocks rallied in both of those years. "The stock market may fare much better than most expect, as the Fed begins to normalize monetary policy," says James Paulsen, Wells Capital Management's chief investment strategist. After all, what spooks investors these days isn't inflation or runaway growth but deflation, and a less anxious Fed signals that the deflation threat is receding.
Already, we're on firmer footing. The Standard & Poor's 500 was at 1469 just this January—the same level where it began this century. Yet earnings and dividends have doubled in the 13 years since, slashing the market's valuation from 30 to 14.7 times what companies earned in the preceding 12 months. Household net worth has repaired with firmer real estate and rallying portfolios, and home building, auto sales, and state tax collections all are improving. Unfortunately—or it is fortunately?—gross-domestic-product growth remains wan enough to keep monetary policy loose.
Many investors also remain underinvested in stocks, although margin debt is climbing, and bearish bets have shrunk to a six-year low. Two of the year's three best sectors—both with gains topping 10%—are health care and consumer staples, another sign that stock buyers remain defensive.
Meanwhile, political impasse has deferred—but not destroyed—economic growth. "Despite uncertain fiscal policy, business confidence is off the lows, and investment is accelerating, if new orders are any indication," notes Sean Darby, Jefferies' chief global equity strategist. Most sectors are using more of their capacity than a year ago, notably electrical equipment, autos, and mining. Business lending is increasing, and Darby expects rising capital expenditures to benefit machinery, truck makers, chip equipment, and factory construction.
Alas, a sustained, secular bull market—one robust enough to withstand economic cycles—remains elusive. The 1982-to-2000 bull run, which was interrupted but not stopped by the 1987 crash, began only after deregulation, loosened labor unions, and grim interest-rate hikes had choked off inflation. "Ultimately, it was the shift from short-term fixes to the righting of long-term imbalances that paved the way for a new secular trend," says Jack Ablin, BMO Private Bank's chief investment officer. Yet despite two grievous recessions since 2000, the government continues to attack our fiscal and debt imbalances "with a putty knife and plaster."
Ablin thinks stocks "can keep going until we run out of short-term policy tools," or until the Fed gets nervous that it's perpetuating an equity bubble. But a decades-long, secular bull will only coincide with "a willingness to tackle the politically unpalatable," he says. "Since politics is oriented toward the short term, the difficult choices will likely be forced on lawmakers by the markets." Tough choices rarely occur when we're celebrating new highs, but another leg down is a different matter.
FINDING TOO MUCH OIL and gas under our rocks has been wonderful for the local economy, but it's straining existing infrastructure and transport (see "Climb Aboard the Oil Train"). That's one reason that pressure is mounting on the Obama administration to allow the 1,179-mile, cross-border Keystone XL pipeline that will link Alberta, Canada, to Steele City, Neb., and the rest of the Keystone network.
Daniel Clifton, Strategas Research's policy analyst, thinks there's a better than 50% chance the pipeline will be approved by late summer, despite environmental objections. If so, this leg could begin operation in late 2015. Better access to Canada's oil sands and crude oil extracted in the Bakken shale will benefit producers, as well as Gulf Coast refiners. Clifton also scoured lobbying disclosure reports for companies pushing hardest for this pipeline. Strategas' list of 15 likely beneficiaries includes Canadian Natural Resources CNQ 1.9408208717785556% Canadian Natural Resources Ltd. U.S.: NYSE USD32.04 0.61 1.9408208717785556% /Date(1475182922206-0500)/ Volume (Delayed 15m) : 3670459 AFTER HOURS USD31.9974 -0.0426000000000002 -0.13295880149812733% Volume (Delayed 15m) : 994 P/E Ratio N/A Market Cap 45350029494.267 Dividend Yield 2.2027622542896537% Rev. per Employee 1405520 More quote details and news » (ticker: CNQ), ConocoPhillips COP 3.508771929824561% ConocoPhillips U.S.: NYSE USD43.66 1.48 3.508771929824561% /Date(1475182851459-0500)/ Volume (Delayed 15m) : 16335140 AFTER HOURS USD43.73 0.0700000000000003 0.1603298213467705% Volume (Delayed 15m) : 227555 P/E Ratio N/A Market Cap 52240141483.9188 Dividend Yield 2.2904260192395784% Rev. per Employee 1515790 More quote details and news » (COP), Deere DE -0.27351647044832916% Deere & Co. U.S.: NYSE USD83.86 -0.23 -0.27351647044832916% /Date(1475182843932-0500)/ Volume (Delayed 15m) : 2706407 AFTER HOURS USD83.86 % Volume (Delayed 15m) : 18106 P/E Ratio 16.805611222444888 Market Cap 26439829267.5549 Dividend Yield 2.861912711662294% Rev. per Employee 469156 More quote details and news » (DE), ExxonMobil XOM -0.5063291139240507% Exxon Mobil Corp. U.S.: NYSE USD86.46 -0.44 -0.5063291139240507% /Date(1475182816577-0500)/ Volume (Delayed 15m) : 18599767 AFTER HOURS USD86.46 % Volume (Delayed 15m) : 455521 P/E Ratio 34.30952380952381 Market Cap 360343882840.957 Dividend Yield 3.4698126301179735% Rev. per Employee 2716960 More quote details and news » (XOM), LyondellBasell LYB 0.5875% LyondellBasell Industries N.V. Cl A U.S.: NYSE USD80.47 0.47 0.5875% /Date(1475182943522-0500)/ Volume (Delayed 15m) : 4185896 AFTER HOURS USD80.47 % Volume (Delayed 15m) : 113266 P/E Ratio 8.683313190607734 Market Cap 33257758789.0625 Dividend Yield 4.225177084627812% Rev. per Employee 2267540 More quote details and news » (LYB), Quanta Services PWR -0.10779734099892202% Quanta Services Inc. U.S.: NYSE USD27.8 -0.03 -0.10779734099892202% /Date(1475182875524-0500)/ Volume (Delayed 15m) : 2349383 AFTER HOURS USD27.8 % Volume (Delayed 15m) : 24394 P/E Ratio 20.441176470588236 Market Cap 4012919015.7933 Dividend Yield N/A Rev. per Employee 299868 More quote details and news » (PWR), TransCanada TRP.T -0.08396305625524769% TransCanada Corp. U.S.: NYSE USD47.6 -0.04 -0.08396305625524769% /Date(1475182921853-0500)/ Volume (Delayed 15m) : 804941 AFTER HOURS USD46.87 -0.730000000000004 -1.5336134453781514% Volume (Delayed 15m) : 1600 P/E Ratio N/A Market Cap 49950822167.8566 Dividend Yield 3.6164825834359244% Rev. per Employee 2016870 More quote details and news » (TRP.Canada), and Valero VLO -6.169479223371439% Valero Energy Corp. U.S.: NYSE USD51.71 -3.4 -6.169479223371439% /Date(1475182922392-0500)/ Volume (Delayed 15m) : 13763844 AFTER HOURS USD52 0.289999999999999 0.5608199574550378% Volume (Delayed 15m) : 143384 P/E Ratio 8.353796445880452 Market Cap 25424502979.9445 Dividend Yield 4.641268613421002% Rev. per Employee 7587250 More quote details and news » (VLO).
E-mail: firstname.lastname@example.com


text:
Penn Championship Tennis Balls & Wristband - 6 Can Pack
Product Information:
Practice your game in the off season or play with friends with the Penn® Championship Tennis Balls 6-pack. Multi-packs of tennis balls are easy to transport to the court in your tennis bag and provide ample equipment for hours of play. A free wristband is included to add a touch of style to your game. USTA and ITF approved.
- Extra Duty tennis balls for hard court surfaces
- Controlled fiber release offers consistent nap
- Natural Rubber for consistent feel and reduced shock
- Interlocked wool fiber for durability and longer wear
- Deep elastic seams for reduced cracking
- USTA and ITF approved
- Penn® Championship Tennis balls are America's #1 Selling Tennis Ball and set the standard by which all other tennis balls are measured--now includes a free HEAD® wristband inside each pack!
- Includes six 3-ball cans plus one free wristband
- Model: 521080
- Penn
Country of Origin: ChinaWeb ID: 3982085
Customer Reviews:
Rated 4.4 out of 5�by 132 reviewers.
Rated 5 out of 5�by KC THEY BOUNCE AND HAVE GOOD ACTION I AM JUST REC TENNIS PLAYER, THE BALL BOUNCED WELL AND HAD GOOD ACTION. MY DOG CHASED AND ATE ONE, HE SEEM TO ENJOY IT. I THINK THE WRIST BAD INCREASED MY POWER :). September 5, 2013
Rated 5 out of 5�by joanie great value for tennis balls at dicks great assortment, and value for tennis balls, shoes, strings, clothing etc. September 2, 2013
Rated 4 out of 5�by charmedRic Decent Deal Aside from one of the cans being sliced open by a careless box-cutter, my guess from unpacking the boxes, this was a good purchase. August 18, 2013
Rated 4 out of 5�by gendotte I just learned something They are tennis balls, so there isn't much that can go wrong there, but since I haven't used them up or fully opened the package, I didn't realize that there is a wristband in there. Guess I had better fish it out before my next match! August 17, 2013
Rated 5 out of 5�by Lee Tennis balls Great value on the multi pack. Need other brands with bulk pricing. July 2, 2013
Rated 4 out of 5�by Phoenix Jim Bounce These balls meet minimum USTA standards; are relatively cheap, and are most suitable for a couple of hours of play. June 18, 2013
Rated 4 out of 5�by MAG Bouncey balls These balls are yellow and they bounce like tennis balls so it is all good. May 14, 2013
Rated 4 out of 5�by Gooch Good Sports Equipment The selection is good and the price is great for the amount of items in set. April 26, 2013
Rated 5 out of 5�by Rishi Penn tennis balls They are awesome and works great on the hardcourt. I am a regular on that April 16, 2013
Rated 5 out of 5�by Love_tennis Bounce Great value for as much as you get when you buy this 6 pack of balls. Love_tennis April 8, 2013


text:
Toulouse School of Economics and Campus channel will be presenting their international master's programme next October.
You are hereHome | Programs | Graduate program
Graduate program
The TSE Masters program
In September 2016, the school will offer two types of Masters :
- A "Master degree in economics" partially taught in French
- A "Master degree in economics" international track entirely taught in English
For more information and admission criteria, click HERE
These masters program includes core courses in the main disciplines (micro, macro and econometrics) as well as a broad choice of electives The objectives are to enhance the students' general economics culture and to prepare them for more quantitatively oriented classes.
During the second year (Master 2), students may choose between several specialties (be aware that there are some "privileged paths between the 1st and the 2nd year):
- Masters taught in partnership
- Masters in Economics
- Applied economics (open on 2017)
- Economic Theory and Econometrics
- Masters in Finance
For the international master track please click here
- School
- Programs
- Research
- International
- Campus life
- Corporate relations
- Alumni


text:
Imaging and quantifying virus fluorescence signals on aquatic aggregates: a new method and its implication for aquatic microbial ecology
The development of accurate methods to detect and enumerate viruses is an important issue in aquatic microbial ecology. In particular, viruses attached to floating aggregates are a largely ignored field both in marine and inland water ecology. Data on the total abundance and the colonization of aggregates by viruses are rare, mainly due to methodological difficulties. In the present study, we used confocal laser scanning microscopy (CLSM) to resolve fluorescence signals of single viruses and bacterial cells in a complex three-dimensional matrix of riverine aggregates. CLSM in combination with different fluorochromes is a very promising approach for obtaining information both on the aggregate architecture and on the spatial distribution of viruses attached to fully hydrated aggregates. Aggregates from the Danube River harbored up to 5.39 × 109 viruses cm−3. We discuss the problems associated with different methods such as sonication or directly counting viruses on aggregates, both combined with epifluorescence microscopy and CLSM, to quantify viruses on suspended particles.
- virus
- aggregate
- extracellular polymeric substances
- glycoconjugate
- river
- confocal laser scanning microscopy
Viruses are highly abundant in aquatic systems, affect phyto- and bacterioplankton growth, may regulate the genetic diversity of their hosts and may potentially influence the cycling of organic carbon and nutrients (see reviews of Wommack & Colwell, 2000; Weinbauer, 2004; Suttle, 2007; Brussaard et al., 2008). In order to understand the potential impact of viruses on microorganisms, it is important to detect and enumerate the viruses. Typically, viral abundance in aquatic systems ranges between <104 and >108 mL−1 (Weinbauer, 2004; Peduzzi & Luef, 2009). In general, fixed total water samples include particles and aggregates; however, it is likely that without specific techniques, viruses attached to aggregates are severely underestimated. Compared with free-living forms, viruses associated with aggregates and sediments have received little attention, perhaps largely due to methodological difficulties. Nevertheless, the occurrence and importance of viruses in sediments (see the review of Danovaro et al., 2008a, b) and on floating aggregates (Peduzzi & Weinbauer, 1993; Simon et al., 2002; Luef et al., 2007; Mari et al., 2007; Peduzzi & Luef, 2008) is documented. So far, viruses associated with aggregates and sediments were analyzed using various techniques such as sonication (Danovaro et al., 2001; Fischer et al., 2005; Duhamel & Jacquet, 2006) or direct observation (Luef et al., 2007), both combined with conventional epifluorescence microscopy (EFM), sonication in combination with flow cytometry (Duhamel & Jacquet, 2006; Riemann & Grossart, 2008) and transmission electron microscopy (TEM; Peduzzi & Weinbauer, 1993). Recently, Mari et al. (2007) described an indirect approach to enumerate viruses inside transparent exopolymeric particles using magnetic isolation and flow cytometry.
In recent years, confocal laser scanning microscopy (CLSM) has been introduced into the field of microbial ecology to investigate three-dimensional (3D) objects such as fully hydrated biofilms and aggregates in both freshwater and marine environments (e.g. Holloway & Cowen, 1997; Neu & Lawrence, 1999; Neu, 2000; Neu et al., 2001). No attempt has yet been made to detect aggregate-associated viruses via CLSM. Significant advantages of CLSM include the elimination of out-of-focus information and the possibility for horizontal and vertical optical thin sectioning of thick, hydrated biological samples. Hence, our investigation applies CLSM in combination with SYBR staining to visualize the 3D distribution of viruses. In addition, we quantify the CLSM data sets of virus signals in fully hydrated riverine aggregates. We then discuss different methods for quantifying viruses associated with aquatic aggregates in combination with advanced imaging techniques.
Materials and methods
Sampling of aggregates
Water samples from the Danube River (Wildungsmauer, Austria, stream kilometer 1894) were taken in early May and late July 2005 at a sampling depth of c. 30 cm. In July, samples were taken after a flood event. Lotic aggregates were sampled in 1-L plexiglass bottles. The samples were always kept at +4 °C until analysis, which was completed within 24 h (Bura et al., 1998).
Extraction of viruses by sonication
To confirm that viruses can also be properly detected by CLSM, we dislodged viruses from aggregates by sonication as follows: we adopted the method used for sediments by Weinbauer et al. (1998) and Danovaro et al. (2001) to enumerate viruses on floating aggregates. Water samples were filtered onto 3-μm isopore membrane filters (TSTP, 25 mm diameter, Millipore) to obtain the particle-associated microbial fraction. Filters were stored in 4.5 mL of 0.02μm filtered water and fixed with formaldehyde (final concentration, 2%). Tetrasodium pyrophosphate (final concentration, 5mM) was added to the samples, which were incubated for 1h on a shaker. The samples were sonicated three times for 1min on ice, pulsing at 40W using a B. Braun Diessel Biotech Sonifier (needle diameter, 4mm; Labsonic U, Melsungen, Germany). The sonication was interrupted for 30s every minute. Afterwards, samples were filtered onto Whatman AnoDisc filters (pore size, 0.02μm; diameter, 25mm, Whatman, Maidstone, UK) and stained with SYBR Green I (Invitrogen, Eugene, OR) according to Noble & Fuhrman (1998). Filters were mounted on glass slides with an antifade mounting solution (Citifluor AF1, Citifluor, London, UK). Viruses were detected under the CLSM.
Staining and CLSM
To label the matrix material of the riverine aggregates, the lectin from Aleuria aurantia (AAL, Vector Laboratories, Burlingame, CA) was used to stain the lectin-specific glycoconjugates of the extracellular polymeric substances (EPS) (Neu, 2000; Neu et al., 2001; Staudt et al., 2003). The lectins were self-labeled with the fluorochrome Cy5 according to the data sheet of the supplier under dim light (Amersham, Buckinghamshire, UK). Briefly, after the purification resin was packed into the column, the lectin-dye solution (50μL of a 1M sodium bicarbonate solution, 0.5mL of a 2mgmL−1 lectin solution and the fluorescence dye were incubated for 1h in the dark at room temperature) was carefully loaded onto the column. After the mixture entered the column resin, an elution buffer was added until the labeled lectin was eluted. Two colored bands, which represent the separation of the labeled lectin from unbound dye, appeared. The first band, which contained the labeled lectin, was collected.
Lectin staining of the EPS glycoconjugates (100μgmL−1) was performed as described previously (Neu, 2000). For staining, the lectin was diluted with deionized water to a final concentration of 0.1mgmL−1 protein. One hundred microliters of this solution were added to each sample and incubated for 20min in the dark at room temperature. The aggregates (size range of 7–198μm) were then carefully washed three times with tap water to remove unbound lectins. For this purpose, tap water was carefully added with a pipette and drawn off with filter paper to avoid resuspension and disruption of the aggregates. The aggregates were never allowed to dry in the air. To detect aggregate-associated bacteria and viruses on the fully hydrated aggregates, the samples were stained with the nucleic acid-specific stain SYBR Green I (Noble & Fuhrman, 1998). After staining the lectin-specific EPS compounds, SYBR Green I (1μLmL−1 deionized water) was directly applied to the aggregates and incubated for 5min in the dark at room temperature. The stained samples were carefully transferred into cover well imaging chambers (0.5mm spacer, Invitrogen), covered with tap water and immediately examined by CLSM.
CLSM was performed using a Leica TCS SP1, controlled by the lcs version 2.61 Build 1537 174192 (Leica, Heidelberg, Germany), equipped with an upright microscope. For detecting viruses, images were taken with a × 100 1.4 NA oil lens. The aggregate structure was analyzed by CLSM using visible lasers (488 and 633nm). Emission signals were detected from 500 to 550nm and from 650 to 750nm. Optical sections of aggregates were taken every 0.2μm.
Cryo-embedding, cryo-sections and CLSM
To quantify viruses and bacteria on riverine aggregates, cryo-sections were also performed. Aggregates from the Danube were embedded in liquid cryostat medium (Neg-50 by Richard-Allan Scientific), frozen at −26°C and physically sectioned (10μm) with a cryomicrotome (Leica CM3050S) (Huang et al., 1996). Thereafter, sections were stained with lectins and a nucleic acid stain, were embedded in water and covered with a coverslip before analysis. The sections of the aggregates were examined by CLSM using a TCS SP2 controlled by the lcs version 2.5 Build 1227 192162 (Leica). Images were collected using an inverted DM IRB microscope and a × 100 1.4NA oil lens. Aggregate structure was analyzed by CLSM using visible lasers (488 and 633nm). Emission signals were detected from 500 to 550nm and from 650 to 750nm.
Quantification and visualization
To quantify virus signals, the freely available software image j (http://rsb.info.nih.gov/ij/), developed in java (Staudt et al., 2004) or, alternatively, the IDL-based program confocal analysis (conan) version 1.31, was applied. The software conan was developed by BioCom for the Helmholtz Centre for Environmental Research – UFZ in Magdeburg.
To quantify lectin signals, the software image j was used. For each aggregate, the threshold was set manually. Because of the very heterogeneous composition of the aggregates, automatic batch processing could not be applied.
To visualize 3D data sets, imaris 4.2 (Bitplane AG, Zurich, Switzerland) was used. Thresholds were set manually. Adobe Photoshop CS2 was used to insert calibration bars into the images.
CLSM and visualization
In a first step, we had to confirm that virus signals can also be detected properly by CLSM. Therefore, samples were sonicated, filtered, stained and analyzed by CLSM (Fig. 1). Viruses and bacteria appeared bright and could be easily distinguished based on size and light intensity. Other than in conventional EFM, almost no background fluorescence was present when using CLSM. To obtain information about the spatial distribution of viruses attached to aggregates, the fully hydrated aggregates were analyzed directly by CLSM after staining. This is the first time that viral, bacterial and glycoconjugate distributions have been visualized together by means of CLSM in fully hydrated aggregates. The serial optical sections, as shown in Fig. 2, provide an insight into the arrangement of viruses, bacteria and the matrix of heterogeneous aggregates. In addition, Fig. 3 shows the presence of viruses and lectin-specific glycoconjugates, their location and also their colocalization in a 3D reconstruction.
CLSM and quantification
The software image j and conan enabled quantification of the lectin-specific glycoconjugates and of the bacteria, but not of the viruses. Although the diverse programs could not identify virus signals correctly, the human eye could easily distinguish between a virus and an occasional background pixel. Nonetheless, counting viruses manually in untreated aggregates was not manageable. The main obstacle was to identify whether the DNA signals were viruses or sections of bacteria: therefore, each section of an image stack had to be compared to determine whether the fluorescence signals were virus-derived or merely a section of a bacterium. Performing this comparison for each fluorescence signal is too laborious, especially for heavily colonized aggregates.
Cryo-sections, however, allowed detecting the distribution of viruses, bacteria and polymeric constituents inside the aggregate with more accurate resolution in a reasonable time (Fig. 4). Potential limitations of the CLSM technique due to scattering, laser penetration and diffusion of staining solutions can be overcome by analysis of cryo-sectioned poststained samples. Abundance data were therefore obtained from cryo-sections of aggregates that were counted manually. The abundances of bacteria and viruses associated with Danube aggregates are summarized in Table 1. In spring, for example, aggregates harbored on average 17.27 × 108bacteriacm−3 and 18.77 × 108virusescm−3 lectin-specific glycoconjugate. In summer, after a flood event, the corresponding average values were 1.55 × 108bacteriacm−3 and 2.48 × 108virusescm−3 lectin-specific glycoconjugate.
|Bacteria (× 108cm−3 specific glycoconjugate)
|Viruses (× 108cm−3 specific glycoconjugate)
|Bacteria (× 108cm−3 specific glycoconjugate)
|Viruses (× 108cm−3 specific glycoconjugate)
VBR, virus to bacterium ratio; n, number of analyzed cryo-sections.
CLSM, visualization and quantification
In recent years, CLSM has become an indispensable tool for studying 3D biofilm and aggregate architectures, their chemical compositions and associated microbial communities (e.g. Böckelmann et al., 2002; Staudt et al., 2003; Neu et al., 2004). To our knowledge, no one has yet attempted to detect viruses attached to aquatic aggregates by CLSM. Generally, interactions between natural virus assemblages and suspended particulate matter are poorly documented. Particularly in riverine systems, where suspended matter is an important factor, very little information is available on the interaction of viruses with aggregates. We therefore used CLSM to resolve single virus signals and bacteria in a complex 3D matrix of riverine aggregates (Figs 2 andFigs 3). In combination with different fluorochromes, this allowed us to obtain information on both the aggregate architecture and the spatial distribution of viruses in fully hydrated aggregates.
Because viruses attached to riverine aggregates were detectable, we attempted to quantify the virus signals. Ample software is available for quantifying CLSM data. This includes freely available as well as commercial software. None of the programs, however, is suitable for multipurpose applications.
One crucial step in digital image analysis is setting thresholds (Xavier et al., 2001; Beyenal et al., 2004). Thresholding is a segmentation method that essentially reduces 256 gray-scale level images to binary images in order to separate the image into biomass (signal) and interstitial space (background). To extract statistically meaningful parameters from image series, the thresholding has to be reproducible. There are no general rules for setting thresholds. The operator uses his or her best judgment, setting the gray-scale level such that the binary image appears to capture the essence of the EPS structure, viral and bacterial signals. Setting the thresholds manually is quite time consuming and variability between operators in choosing the threshold adversely affects the measurements obtained from the binary image. Most computer programs are equipped with automatic image-thresholding procedures. Such procedures must be tested carefully before use. Changes in thresholding can alter the numerical values of diverse parameters (Beyenal et al., 2004). We tried different automated methods of thresholding. As the aggregate structures were so heterogeneous, the computer programs did not have a sufficiently precise automatic procedure to yield reproducible results compared with the human operator.
Setting the thresholds manually, the computer programs conan and image j allowed us to estimate bacterial and EPS volumes, but it was not possible to quantify viruses accurately. Two important points have to be considered for digital image analysis and especially for virus quantification: firstly, virus signal intensity vs. background signal, and secondly, virus signal size vs. pixel size. The above computer programs failed to distinguish between virus and background pixels, probably because the algorithms were insufficiently sensitive. Viruses and bacteria attached to aggregates were therefore counted manually. Nevertheless, CLSM is recommended as the method of choice to resolve single viruses in a complex 3D matrix of fully hydrated riverine aggregates. Although more sensitive and precise algorithms for thresholding may become available, thresholding remains difficult because riverine aggregates have a very complex composition.
Comparison of different methods to quantify viruses and bacteria attached to riverine aggregates
Direct counts provide a wealth of basic information on viruses in aquatic ecosystems. This calls for accurate methods to detect and enumerate viruses on particles as well. At least three approaches have been used for such enumerations: (1) TEM, (2) DNA/RNA staining techniques using EFM or flow cytometry and (3) magnetic isolation combined with flow cytometry.
Using TEM, for example, riverine aggregates can be embedded in Nanoplast in combination with uranylacetate counter-staining (Leppard et al., 1996). TEM offers a high resolution and therefore clearly identifies accumulations of viruses. However, TEM requires fixation and/or dehydration in order to examine the 3D aggregate structure. Both processes can produce severe artifacts, especially in highly hydrated samples. Moreover, TEM is very time consuming and expensive.
Sonication is widely used to dislodge viruses from sediments, aggregates, etc., combined with EFM and flow cytometry (e.g. see the review of Danovaro et al., 2008 a; Riemann & Grossart, 2008). Using CLSM, we also accurately detected dislodged viruses (Fig. 1). However, sonication appears to be a useful method for reasonably quantifying viruses on floating aggregates, but does not supply any information on the spatial distribution of viruses on the aggregates.
Luef et al. (2007) directly observed particle-attached viruses and bacteria in combination with conventional EFM. This technique has limitations in distinguishing between stained viruses and background fluorescence from deeper layers of the aggregate. Occasional high densities of attached bacteria also hampered the precise detection of single virus signals. Examining a sample by EFM required frequently adjusting the plane of focus. Furthermore, bacteria and viruses underneath the aggregates or deeper inside were undetectable.
The major disadvantage of fluorescence microscope techniques is image degradation by out-of-focus information originating from focal planes located above or below the objects of interest. CLSM systems provide the opportunity to extend light microscopy studies beyond the limitations of traditional EFM (Lawrence et al., 2002, 2007).
For CLSM-based quantification, the entire aggregates were not analyzed. Riverine aggregates incorporate a high amount of noncellular matter such as sand, clay and detritus, which limit diffusion of the staining solution, laser penetration and detection of emission signals in thick samples. Thus, large aggregates had to be embedded and physically sectioned into slices using cryo-sectioning. Cryo-sections allow much better detection of the distribution of viruses, bacteria and polymeric constituents inside the aggregates. From each slice, bacterial and viral abundances were counted manually due to the above-mentioned difficulties when using digital image analysis.
CLSM allowed clear recognition of virus accumulations (Fig. 5), although sometimes the quantification of single virus signals and clear identification within such accumulations was still hampered. It was also occasionally difficult to distinguish single virus particles in aggregates containing high densities of stained cells and/or a matrix that was also stainable with nucleic acid dyes. Nevertheless, CLSM is a very promising approach for obtaining the abundances of viruses attached to or within aggregates.
The resolution of any linear imaging system is given by its point spread function, which quantifies the blur of an object point in the image. The sharper the point spread function, the better the resolution. In 1873, Ernst Abbe discovered that lens-based optical microscopes cannot resolve objects that are closer together than half of the wavelength of light. Recently, however, Schmidt et al. (2008) introduced stimulated emission depletion microscopy with 4π geometry that creates nearly spherical focal spots 40–45nm (λ/16) in diameter. The combination of these techniques will probably push the z resolution to <10nm (Hell, 2007). Very likely, such an approach, which sharpens the point spread function, will yield even better resolution/images of viruses attached to aggregates.
Interactions of viruses and particulate material
The literature on the interaction between suspended matter and the natural assemblage of viruses infecting microplankton organisms, such as bacteria and phytoplankton, is surprisingly scarce. Particularly in freshwater systems, where suspended matter is often a prominent factor, very little information is available on the interaction of viruses with aggregate-associated host bacteria. However, even for marine systems, we know little about virus–particle interactions.
Few studies on the abundance of viruses attached to or within organic aggregates are available (Peduzzi & Weinbauer, 1993; Simon et al., 2002; Luef et al., 2007; Mari et al., 2007; Peduzzi & Luef, 2008). Marine snow particles from the Northern Adriatic Sea harbored 5.6 × 1010viralparticlescm−3 aggregate based on ultrathin sections and subsequent TEM investigations (Peduzzi & Weinbauer, 1993). In the present study, up to 5.39 × 109virusescm−3 lectin-specific glycoconjugate were found in the Danube when analyzed by CLSM (Table 1). A comprehensive overview of studies on viruses attached to different substrata in inland waters is given by Peduzzi & Luef (2009). For example, Luef et al. (2007) and Peduzzi & Luef (2008) showed that 0.01–0.89 × 107virusesmL−1 water were attached to particles in the Danube and its floodplain system. In the Talladega Wetland, Alabama, viruses attached to surfaces ranged from 1.3 × 106virusparticlescm−2 on macrophytes to 1.1 × 107 virusparticlescm−2 on wood (Farnell-Jackson & Ward, 2003). In the Mahoning River, Ohio, viral abundances ranged from 1.65 to 6.68 × 108g−1 ash-free dry mass (Lemke et al., 1997), leaves harbored 4.81–21.8 × 108virusesg−1 and sediment samples 4.71–8.91 × 106virusesg−1 (Baker & Leff, 2004). At Lake Hallwil, Switzerland, 0.2–0.9 × 108 virusescm−2 biofilm, 3.5–10.6 × 107virusesmg−1Corg plant litter and 1.9–5.3 × 109virusescm−3 sediment were found (Filippini et al., 2006). Furthermore, quite recently, Danovaro et al. (2008a) gave a broad overview of viruses in both freshwater and marine sediments. In inland waters, viral abundances range from >0.01 to 203.3 × 108virusesg−1 dry sediment. A literature comparison of viral abundances associated with aggregates, biofilms, sediments, etc. is very difficult due to the different methods used and the variable units presented. Nonetheless, such associated viruses apparently reveal some dependence on the type of the particulate matter, such as particle size and quality (Lemke et al., 1997; Farnell- Jackson & Ward, 2003; Luef et al., 2007).
The presence of solid surfaces in the water column implies several ecological consequences. Flood & Ashbolt (2000) observed that wetland biofilms could entrap viral-sized particles and concentrate them over 100-fold compared with abundances in the surrounding water column.
In a study on viral decay in the Gulf of Mexico, free-living viruses may bind irreversibly to loosely associated aggregates, thereby losing their infectivity (Suttle & Chen, 1992). On the other hand, viral association with colloidal and particulate materials can prolong their survival (Kapuscinski & Michell, 1980), and phage production and transduction frequencies can increase in the presence of particulate matter (Kokjohn et al., 1991; Ripp & Miller, 1995).
Using a model system, Riemann & Grossart (2008) showed that a marine phage may effectively lyse surface-attached bacteria. Enhanced lytic production of viruses may also occur in natural systems with high particle loads, due to elevated viral abundances found on transparent exopolymeric particles (Mari et al., 2007) and on some riverine particles (Luef et al., 2007). Therefore, aggregates may represent hot spots for viral infection of bacteria. Bacterial lysis mediated by viruses on aggregates may be an important mechanism to release dissolved organic matter into the water column. Furthermore, increased viral production on aggregates may also lead to increased release into the surrounding water, which may significantly alter the distribution and community composition of viruses in the whole system. However, solid surfaces can also adsorb virus particles, thus reducing viral infection while increasing prokaryotic production (L. Kernegger, I. Zweimüller & P. Peduzzi, unpublished data).
Viral infection of bacterial cells attached to aggregates may be impeded by various structures, including the bacterial extracellular polymers. Specific phages can degrade susceptible biofilms and continue to infect biofilm bacteria during the degradation of EPS (Hughes et al., 1998 a,1998 b). Many phages, but not all, may use polysaccharases or polysaccharide lyases (Hughes et al., 1998 a,b; Sutherland et al., 2004). Viruses can indirectly produce EPS by lysing bacteria and phytoplankton biomass and may play an important role in flocculation processes (Peduzzi & Weinbauer, 1993). The formation of aggregates or the attachment of viruses can also have biogeochemical consequences when considering vertical or horizontal transport of such material in the various aquatic systems. Thus, viruses certainly can be nanoscale drivers in ecological and biogeochemical processes.
Based on the above-outlined information, it becomes evident that visualizing and quantifying virus distribution on particles is important in microbial ecology of floating aggregates in aquatic systems. This new technique should contribute, among others, to elucidate the significance of viruses on suspended matter.
CLSM, in combination with different fluorochromes, represents an in situ approach that yields information both on the architecture of aggregates and on the spatial distribution of bacteria, viruses and polymeric constituents. For the first time, viral, bacterial and glycoconjugate distributions could be visualized by means of CLSM in fully hydrated riverine aggregates. Although some difficulties occurred when quantifying viruses attached to aggregates, CLSM is proposed as the method of choice for investigating multiple constituents in fully hydrated suspended matter.
We thank the Austrian Science Foundation FWF (grant numbers P14721 and P17798 to P.P.) for financial support. We are grateful for the technical expertise and help by U. Kuhlicke.
Editor:Riks Laanbroek
- © 2009 Federation of European Microbiological Societies.


text: For this noteworthy release, Nike merges its signature Flyknit tech with the widely popular Roshe Run silhouette. The Flyknit upper is finished with a honeycomb overlay in a clean black and grey colorway, while the sole gets a slight rework also, featuring a more acute berm that runs the length of the sneaker. Filed under Nike's "Natural Motion" silo, this new hybrid drop is sure to be sought after by fans, and could be the forebearer of additional Flyknit Roshe mockups. For those interested, check out Slam Jam for availability and pricing.


text:
They call me Dalton Ross and say I cover TV for Entertainment Weekly and EW.com. Hope they're right.
Face Tags
Love when visitors doodle on my whiteboard. Check out Aaron Paul and Andrew Lincoln's handiwork.
Big props to @LD425 for helping me replace my favorite hat!
Okay @MarleeMatlin and @johnrich fans, choose my lunch beverage for me. Most votes wins!
Boy, I'm thirsty...
My favorite piece of luggage...EVER!
Which record should I listen to next here at work? You pick for me!
One book I will not be buying today.
Trying to decide which junk food to plow through first at our EW American Idol photo shoot.
How much will you pay me to blast this record throughout the EW hallways right now?
Otis Redding won round 2. Now which of these albums should I listen to next? You decide, I listen.
Okay, Tears for Fears won round 1. Now tell me which record to listen to next. You vote, I listen.
Brought my old records to work today. Which one should I listen to next? You decide for me.
@parvatishallow, @erikalynnshay Your newest east coast rep/yoga enthusiast
The one good thing about all the snow? Dorking out and re enacting the big Hoth battle scene.
Jose Canseco just made me a pizza! No Roids toppings though. #celebrityapprentice
Stormtroopers it is!
Here's a clearer picture of the new Survivor: Nicaragua buff.
Hangin' with @JennaMorasca, who models the new Survivor: Nicaragua buff!
Russell Hantz at Comic-Con...kinda. Plus, a dude caught picking his nose
Maybe I should sign @EWAnnieBarrett up!
©2016 Twitpic Inc, All Rights Reserved


text:
Light the way towards your logo with this colorful promotional key light.
Have a ball with these brightly colored custom stress balls!
A promotional tape measure for the handyman at home or professional tradesmen.
The unique round container of this well rounded Promotional Lip Balm will make a trendy giveaway for your next event.
Catch the spirit of patriotism and this large Patriotic Stars Promotional Beach Ball with your company logo!
Our logo spring thing is a fun childhood favorite with your logo on it! Compare to Slinky.
Make an investMINT in your brand and get excellent flavor promotional mints at a great price.
The Ball of Whacks Promotional Creativity Tool is a brilliant creativity tool, created by Roger von Oech, a well-known creativity expert.
Whether you're cruising to the office or out on a weekend getaway this custom travel mug is perfect for your daily beverage.


text:
artsea kara
CrossFit Tidal Wave's first annual Barbells for Boobs was a huge success! It was amazing to see so many friendly faces as well as new ones! Tidal Wave was honored to host an event for such a good cause that hits so close to home. Thanks to all our sponsors, volunteers and everyone who came out on such a cold day--for Galveston :)
We hope you will take part in this event next year...we are already thinking of ways to make it even better!!!
--Be a Force of Nature--


text:
Top Plays: Duke 95, NC State 60
Rasheed Sulaimon hosts Duke Hoop v. 3.2:
President Bush visits Duke:
- Sulaimon was odd man out as Duke's No. 3 option; he wants inIn the NBA, they call it DNP-CD: Did Not Play-Coach's Decision. If being singled out for such a designation seems cruel, college is worse. They just exclude your name from the box score. You don't play, you don't exist. Except if you're a former McDonald's All-American and ACC all-freshman choice, in which case they talk on television for two hours about why you've disappeared.
- Rookie Ryan Kelly Leads Lakers Over Celtics: Kelly finished with 20 points in 34 minutes--both career highs. He also had four rebounds, including one especially crucial rebound that gave the Lakers the ball with 25 seconds to play.
- Turnovers help Duke cruise past NC State: This looked like some of the great Duke's teams of the past crushing an outmatched opponent at home, whether it be a non-conference opponent or an ACC team that is a bit young -- like the Wolfpack this year -- and simply breaking their spirit.
- George H. W. Bush cheers on victorious Duke Basketball: Duke trounced North Carolina State in Saturday's basketball game, but the crowd favorite was former President George H. W. Bush, who was greeted with a standing ovation and chants of "USA."
- resident George H. W. Bush Salutes Duke's Mike Krzyzewski for Commitment and Leadership Toward Eliminating Cancer: The 41st President of the United States, George H.W. Bush, traveled to Cameron Indoor Stadium on the campus of Duke University in Durham, NC to recognize Duke men's basketball head coach, Mike Krzyzewski, for his personal commitment and leadership in working toward eliminating cancer as a public health threat.
- Duke Basketball: Takeaways from the Win over Virginia: It looked like the Duke basketball team was well on its way to an impressive bounce back win over Virginia. The Blue Devils used depth and intensity to overwhelm UVA for much of the night and held an 11 point lead with 3:44 remaining in the game.
- Crowder Receives Duke Football MVP Honors : Junior wide receiver Jamison Crowder was named the recipient of the Carmen Falcone Most Valuable Player Award at Duke University's annual football banquet held on Friday evening at the Washington Duke Inn & Golf Club in Durham, N.C.
- Cutcliffe Earns AFCA National COY Honors: Duke head football coach David Cutcliffe has been named the 2013 National Coach of the Year by the American Football Coaches Association, announced on Monday at the organization's annual convention in Indianapolis, Ind.
- Season wrap: Duke: Duke coach David Cutcliffe has made a recent habit of saying that the Blue Devils are here to stay. If it means more seasons like 2013 are on the horizon, those record books in Durham, N.C., could be re-written an awful lot in the coming years. Duke put together a historic season in 2013, winning eight straight games to go 10-4 (6-2 in the ACC) and win the Coastal Division title before dropping its final two games, against the two most recent Heisman Trophy winners.
- Vitale Calls Jahlil Okafor "Best Legit Post Player" Duke Blue Devils Have Ever Had: You could call Dick Vitale a man of hyperbole, but you would also have to say that he has seen quite a bit of college basketball in his years. It did not come as a surprise to me then, when the ESPN analyst called Jahlil Okafor the (in all caps of course) "BEST LEGIT POST PLAYER" the Duke Blue Devils have ever had.
- Duke lands Florida DT prospect: Sources confirmed that Edgar Cerenord has committed to Duke following his official visit to Durham
I Had a great time up at Duke!!! Sad that I'm leaving now.... but I'll be back in May #DukeGang14 #BlueDevils #DukeNation ???????????— EA11 (@Boss_EAC) January 19, 2014
I will be taking an official visit to Duke on March 8th for the Duke vs UNC game !!— Chase Jeter (@chasejeter04) January 15, 2014
On my tippy toes lol , but 5 of 8 linemen commits ???????? pic.twitter.com/5BMue7iqvM— shaun wilson (@To_Dope1) January 19, 2014
I love Duke I love Durham I love the staff I love the players I love the facilities I love the students— CT17 (@iamCT5) January 19, 2014
Ten wins? Check. Ten consecutive semesters with a team GPA of 3.0+? Check. Congratulations to the 2013 team for accomplishing another goal!— Duke Football (@Duke_FB) January 13, 2014
Once in a lifetime opportunity http://t.co/ZRR2tlXUCq— Rasheed Sulaimon (@sheed_ctmd1) January 19, 2014


text:
You may also like
Ratings for Dr. William B. Geissler
Doc did 2 hemiarthroplasty on me. My shoulders were always hurting and limited motion due to arthritis. I am back competing in sports and am pain free. Great range of motion. Very pleased with results. I highly recommend the Doc and his staff.
I have been coming here since March. Staff has not put me down for two scheduled appts. I waited 4 hours one time usual wait 2 hrs
Dr. Geissler is as sweet as he can be but make time for your appointment. We had a 3:30 and weren't seen until 5:30. Diabetics take note, bring a snack. Unfortunately, they take you back there for most of that wait, I wish they would leave you up front so you can get to the snack area and walk around and the seating is better. You may can solve the issue by taking a late appointment, they have to close eventually. It's a shame too because he is so nice, the staff too, but yeah if you are one of those people who can't make the time to wait for whatever reason move on this doc is not for you.
Dr. Geissler performed a total elbow replacement on me. I drove from east Texas to Mississippi for this procedure, and I was very pleased with the doctor and his staff ! He is a very talented man !
Dr. William B. Geissler's Credentials
Facility Affiliations
Accepting New Patients
- Tulane University School Of Medicine (Grad. 1985)
Other Specialties
Areas of Expertise
Awards & Recognitions
Publications & Research
Insurance accepted by this Doctor
Top Hospitals in Jackson, MS
Top Urgent Care Centers in Jackson, MS
Find a Doctor by Specialty
- Ear Nose and Throat (ENT) Doctor
- Emergency Room Doctor
- Endocrinologist
- Endodontist (root canal)
- Family Doctor / G.P.
- Gastroenterologist
- Geneticist
- Gynecologist (OBGYN)
- Infectious Disease Specialist
- Internist / Geriatrician
- Massage Therapist (registered or licensed)
- Midwife
- Naturopath
- View all Specialties >
Featured News
You may also like
Top Orthopedic Surgeons
#1 of 98
#2 of 98
#3 of 98
#4 of 98
#5 of 98


text:
Starting Your Own Business
Learn how to start, finance, promote and manage your new company today.
As seen in the SBA's Resource Guide, Gold Alliance Group creates information products that help you to start, grow and improve the profitability of your new company. Begin learning...
Looking for seed capital or other financing solutions?: Don't confine yourself to traditional sources like banks. Learn about new and important sources of lending and equity financing. New online lenders and crowdfunding sites are now options, allowing entrepreneurs like you to receive funding from people around the world who believe in your vision.
What marketing strategies will bring in more customers without breaking the bank? This is the dilemma most new owners face. Here are some low-cost, yet effective ways to get the word out while staying within your marketing budget:
In the growth stage, you need to continuously improve your company's processes and measure your performance in order to manage it better. As an owner, it is impossible to achieve the success you are striving for if you don't focus on what drives your profits and establish processes that work well together to improve cash flow and operations.
Most entrepreneurs struggle to become more productive and efficient with their time. One of the best ways to manage your time wisely is to use automation, outsourcing and apps that assist in day-to-day operations. See essential online management tools.
Tools Recommended for You:
PDF eBooks: New Owner Handbook | Creative Financing | Blogging Guide
Spreadsheets: Accounts Receivable | Pro Forma | Income & Expenses
Templates: Employee Handbook
Courses: Market Research Video Course | Become a Learning Member: Get 1400+ Online Business Courses
If you are an aspiring or new small business owner, you want to maximize profit, minimize mistakes and launch your startup from the best foundation possible. To make sure your strategy is solid, be sure to get this handbook. Read it now, and read it again one year from now. Your bottom line will thank you.


text:
Let's just trade her to the liberals. Based on the column she published yesterday on Town Hall as "Torture by Another Name," and again today in The Arizona Republic as "By defining torture, we define ourselves" (there are some stylistic differences between the two columns), she thinks like they do. Like any liberal, she started with her conclusion, then she assembled the "evidence" she wanted to support it. Her premise is introduced by the following exchange:
Several years ago, I asked a veteran journalist for advice.
"I'm trying to figure out if I have an ethical conflict," I began.
"If you have to ask, you do," he said.
Simple as that. In posing a question, we often reveal the answer.
Apply the same construct to torture. If we have to ask, it probably is.
Actually, it isn't that simple. In the case of, "How much does that yacht cost?" the answer may well be, "If you have to ask, you can't afford it," but not in the case she wants to consider. I could assert with as much logic that "if you have to ask, it probably isn't."
Her argument continues:
Bush administration lawyers tortured the English language trying to justify the unjustifiable.
"Enhanced interrogation" wasn't really torture, they decided, as long as the pain administered didn't result in "death, organ failure, or serious impairment of bodily functions."
By that definition, waterboarding -- the simulated drowning technique favored by Inquisitors ferreting out heretics -- wasn't torture. People might feel like they were going to die, but they weren't really, and so ...
Her ellipsis implies that they were wrong, but were they? That is the question, and her preconceived notion that they were doesn't make it so.
The rest of her article continues with similar mush-brained illogic, including appropriating partial quotes from Lindsey Graham and Alan Dershowitz. In all of them she applies her expressed conclusion that waterboarding is torture, so no further analysis of the question is necessary. But what happens if we entertain the possibility that waterboarding and the other enhanced techniques may not be torture?
Then, her quotes take on a different hue. Graham:
Either we're going to use torture or we're not. And when you say, we won't use torture, unless we think we really, really need it (then) we're not a rule-of-law nation.
Notice that he is speaking about torture in general. And Graham is definitely (at that time, anyway) one of those people who agree with Parker that waterboarding is torture. But if he is wrong his comments still stand, they just don't apply to the current dispute.
Yet, his concern is not with defining torture, it's with the need to abide by laws, not the situational whims of men. To me, that requires that torture be defined by more than "If you have to ask, it's torture." It also means that to ask the question is to try to find the line beyond which you may not go, but within which your actions are legal and ethical and even moral. It also implies that laws can be written which erase even that line, such as a law authorizing the President to take any measure necessary to protect the United States from attack under cerain circumstances.
Or, it implies that Graham would allow thousands to die in exchange for upholding a principle. It's OK for him to believe that, but he should at least acknowledge that that is his position. And so should Parker.
But in the same interview, Graham says
Every military lawyer I've ever met believes that this is vital for the safety of our troops .... I can give you dozens of example of cases involving captured Americans where abuse stopped at a certain point because the people doing it were afraid of being prosecuted as a war criminal ... During the Somalia conflict, they had one of our helicopter pilots. We dropped leaflets all over Mogadishu telling everybody, all the militia people, that we were watching, and that anybody who abuses this person will be a war criminal and we will come after you ...
At this point I have to ask, "How much good did that do?" Who knows? And those people weren't engaging in torture, anyway. It was murder, plain and simple.
Let's do it this way, Kathleen. Let's define what torture is, then ask if a given technique meets that criteria. Let's not just say, "If we have to ask, it probably is--or isn't."