text:
Skip to comments.Bob Woodward: World Doesn't See America As 'The Grown-up Nation' Anymore
Posted on 07/10/2011 7:36:55 AM PDT by Sub-Driver
Bob Woodward: World Doesn't See America As 'The Grown-up Nation' Anymore By Noel Sheppard Created 07/10/2011 - 12:48am
Bob Woodward thinks the world doesn't hold the United States in very high regard anymore.
Appearing on the syndicated "Chris Matthews Show" this weekend, the Washington Post's most recognizable journalist said, "Im not sure the United States has been looked at as the grown-up nation for a long time...You travel around the world a little bit, and, and theres, theres not even tough love for the United States" (video follows with transcript and commentary):
BOB WOODWARD, WASHINGTON POST: But on your question about grown-up, Im not sure the United States has been looked at as the grown-up nation for a long time. I think, I think theres a lot of negativity out there.
You travel around the world a little bit, and, and theres, theres not even tough love for the United States.
In the circles someone like Woodward travels in, is there ever a high regard for America?
Was there respect for this country amongst his peers when he was reporting on Watergate almost 40 years ago?
It really is nothing new that America's journalists hold her in such disregard. They've been doing it for decades.
But not all of them. The Chicago Tribune's Clarence Page wasn't necessarily willing to sell America short alongside Woodward:
CLARENCE PAGE, CHICAGO TRIBUNE: And yet look how calm Wall Street has been. I mean, there, its, theres a sense on Wall Street that there are a lot of theatrics going on here, but that the United States is not going to default.
Maybe one of the greatest measures of the respect the world has for a nation is their willingness to invest in its debt.
With all the financial distress and calamities that have befallen the globe in recent years, America's Treasury paper continues to be the universal safe haven of choice:
Above is a five year chart of our 10-year Treasury note. The vertical axis is interest rate, the horizontal one years.
For those unfamiliar, bonds and notes trade in an inverse relationship to interest rates. This means the lower the rate, the higher the price.
As is plainly evident, since the financial world turned on its ear in 2007, international investors have continually poured more and more money into our debt.
Contrary to Woodward's world view, this means that people with real money - maybe not the folks he hangs out with - do indeed see America as the grown-up.
If they didn't, they'd be piling out of our debt with both hands.
On the other hand, assuming the great-unwashed around the world aren't pleased with America, wasn't the election of Barack Obama going to change all that?
Didn't folks like Matthews and Woodward claim the junior senator from Illinois was going to radically improve our nation's level of respect across the globe?
Not at all surprisingly, this didn't come up during this discussion of the world not seeing America as the grown-up nation anymore.
Must be because that's George W. Bush's fault despite him being out of office for two and a half years.
Forgive me for even mentioning it.
well there is the man child in the white house.
Why would they, when we elected a child to the Presidency?
Part of the problem is in caring how we are viewed in the first place.
Well Bob, that must mean Barry's apology and bowing tour was a dismal failure!
Ditto: plus Woodward is a senile putz head.
We AREN'T!! Most of the people know more about American Idol than anything about government....Plus 48% don't pay any taxes and have no skin in the game.....America is just an address for them.
Wait... I thought the world hating us was all Bush's fault, and the Blue-Lipped Kenyan was going to *change* the worldview towards us...
You mean he *failed* at that *also*?
its the end result of putting adolesant marxists in charge.....
Don't forget all the narcissistic liberals looking for their 'inner child'...
ALSO, ALSO, ALSO, ALSO, and so on!
Do you really believe the only tax in America is the PERSONAL INCOME TAX?
That's ok Bob, nobody sees you as a grown up journalist.
Liberal stooge.
No nation that is run by liberals can be considered "mature." Ultimately, it ends in catechism, and that is when it when it reaches maturity.
Bob Woodward is reduced to picking scabs. That's how irrelevant he has become.
I read recently how the US State Dept was in a spat with El Salvador because the US Govt is promoting Gay Marriage there.
We haits only one example, but we have lost our way and the world sees it.
It's just so crazy. The left relentlessly slanders the United States for decades, then complains when the slander works.
With an admistration acting like little middle school 12 year olds, why should the world give us any respect? We obviously don't respect America enough to elect responsible adults to care for her.
It's childish to go on insane spending sprees and address how to pay for it after the fact. And on and on and on. What has our POTUS done that isn't childish? His supporters have the same mentality.
Ann Archy might just not have stated that the 48% she refers to aren't smart enough to recognize that fact.
It's really not nonsense.
Remember, after WW2 the rest of the world (outside of the US) was essentially destroyed. All the US had to do was manufacture and produce products. In fact, in the early 1950's the US produced over 50% of the entire world's GDP....that's astounding!
So, after an additional 50 years the rest of the world is catching up....end of story.
Bob no one gives a flying flip what you think, bud
I truly do not know a man who would allow themselves to look like that, never mind being photographed.
If that were true, I'd say mission accomplished Bob.
F U Bob Woodward, and that sweaty mare you road in on too, Katherine Graham.
Wow! What an amazing coincidence! I woke up just this morning wondering what Bob Woodword's opinion is of the state of our nation and its image overseas.
Then I flushed and the thought suddenly vanished.
Present Day America like the wimpy teen boy who's too busy trying to be 'friends' with the hot cheerleader, hoping that if he's 'friends with her', kisses her arse and let's her walk all over him, he'll one day get into her 'delicates'.
We have regressed from a man who takes charge to a metro sexual more concerned about their personal appearance.
wonder why?
This asshole helped create what he now decries.
America is in psychosis and may never recover. Why should anyone aspire to this?
America is in psychosis and may never recover. Why should anyone aspire to this?
After 50 plus years the world has caught up. The student surpassed the teacher.
I happen to know some of these people that pay NO INCOME TAXES and GET MONEY BACK....THOUSANDS OF DOLLARS of OUR MONEY!!
I Pray you aren't one of these BLOODSUCKERS.
Promoting gay marriage is part of the USA's foreign policy now. They're even threatening to cut off any nation that doesn't make moves to start supporting it. Think that'll create more enemies than ruining the EU's gravy train with Iraq . . . ?
I read recently how the US State Dept was in a spat with El Salvador because the US Govt is promoting Gay Marriage there
...how could they with this illegal alien infesting the WH who conducts himself like a teenager of the age of a 14 year old...advocates violence by his brothers...the Enosh.
I spoke to an old friend, this morning, who really follows politics and despises Obama. He's convinced that once he's out of office we'll learn he's gay.
The world sees a celebrity, a "pop star", not a world leader in the WH.
FICA is charged against all wage earners ~ it is paid to those who live long enough who otherwise qualify.
If you never paid FICA you will probably not qualify. If you die you will not qualify.
There are, alas, those who confound Social Security with Supplemental Social Security. SSS is paid out of general revenues. Social Security is paid out of funds received currently ~ although there's a massive multi-trillion dollar overhang where people have paid FAR MORE INTO Social Security than is being taken out.
You aren't one of those people who thinks SSS is paid out of FICA are you?
We've got sophomoric adolescent children in government and our culture is epic in its perversion.
its tough to look at the US as a grown up nation when the POTUS is a child...
Of course we're "not grown up" We won't do, vote, or believe anything he wants us to?!!!
The world is still a huge place. He should define what section of that world is. This being as half of 'em that criticize the United States have their greedy,itchy, larcenous little paws out, for the good old green back.
Oh, make that last sentence read not half of 'em, but most of 'em.
We already know that
You might find shit that is magnitudes worse than what Nixon did.
What have you done lately, Bob?
Disclaimer: Opinions posted on Free Republic are those of the individual posters and do not necessarily represent the opinion of Free Republic or its management. All materials posted herein are protected by copyright law and the exemption for fair use of copyrighted works.


text:
Bastion Hotel Schiphol Hoofddorp - Hoofddorp
Bastion Hotel Schiphol Hoofddorp is a 3-star hotel close to the Amsterdam airport, offering a personal atmosphere, hospitality, comfort, modern facilities, as well as attractive rates.
Thanks to its location along the road around Hoofddorp and near the A4, this convenient Amsterdam hotel is the ideal spot for travelers who need a comfortable place to stay near the airport, where they will enjoy modern facilities to meet the needs of both business and leisure travelers.
This hotel offers free parking, a 24-hour front desk, a bar, and a restaurant which serves an extensive choice of dishes from the à la carte menu. There's also a lounge area with LCD TV, air-conditioning, and relaxing armchairs. The hotel offers free WiFi, as well as an Internet Point.
Bastion Hotel Schiphol Hoofddorp - Hoofddorp
Hotel Info: 1-877-214-4288from $68
- Comfort Room, 2 Single Beds
Also popular with travelers from the USA
At a glance
Key facts
Hotel size
- This hotel has 80 rooms
- This hotel is arranged over 2 floors
- 24-hour check-in
- Check-out time is noon
- Express check-in/out
Required at check in
- Credit card or cash deposit required
- Government-issued photo ID required
Travelling with others
- No pets or service animals allowed
- Free WiFi in public areas
- Free WiFi in rooms
- Free self parking
In the hotel
- Breakfast (surcharge)
- Restaurant
- Bar/lounge
- Coffee shop/café
- Billiards or pool table
- Computer station
- 24-hour front desk
- Dry cleaning/laundry service
- Free newspapers in lobby
- Luggage storage
- Multilingual staff
- Number of buildings/towers - 1
- Designated smoking areas
- Terrace
- Television in common areas
In the room
- Air conditioning
- Coffee/tea maker
- Iron/ironing board (on request)
- Private bathroom
- Shower only
- Free toiletries
- Satellite TV channels
- Desk
- Free WiFi
- Phone
- Refrigerator
- Free bottled water
- Daily housekeeping
- In-room safe
Special features
Awards and affiliations
Green / Sustainable Property
This property participates in Green Globe, a program that measures the property's impact on one or more of the following: environment, community, cultural-heritage, the local economy.
Bastion Hotel Schiphol Hoofddorp - Hoofddorp's small print
Also known as
- Bastion Hotel
- Bastion Hotel Schiphol/ Hoofddorp
- Bastion Schiphol/ Hoofddorp
- Bastion Hotel Schiphol Hoofddorp
- Bastion Hotel Schiphol
- Bastion Schiphol Hoofddorp
- Bastion Schiphol
Special cancellation policies or charges may apply for group reservations (more than 8 rooms for the same hotel / stay dates).
Mandatory fees
You'll be asked to pay the following charges at check in or check out:
- A tax is imposed by the city: EUR 5.00 per accommodation, per night
Optional extras
Breakfast is offered for an extra charge of EUR 14.00 per person (approximately)
We have included all charges provided to us by this hotel. However, charges can vary, for example, based on length of stay or the room you book.

subset: commoncrawl


text:
Aloha Hotel, Manila
- Free WiFi
Main amenities
- 96 guestrooms
- 2 restaurants
- Breakfast available
- Self parking
- Spa services
- Business center
- Airport shuttle
- 24-hour front desk
- Air conditioning
- Daily housekeeping
- Laundry service
- Conference space
For families
- Cribs/infant beds (surcharge)
- Rollaway/extra beds (surcharge)
- Refrigerator
- Private bathroom
- Television
- Free toiletries
What's around
- In Ermita
- Baywalk (0.5 mi / 0.8 km)
- Rizal Park (1.5 mi / 2.3 km)
- SM Mall of Asia (2.6 mi / 4.2 km)
- San Agustin Church (1.9 mi / 3.1 km)
- Manila Yacht Club (0.2 mi / 0.3 km)
- Manila (MNL-Ninoy Aquino Intl.) 21-min drive; 6.1 miles/9.8 km
- Manila San Andres Station 27-min walk; 1.3 miles/2.1 km
- Manila Paco Station 29-min walk; 1.4 miles/2.3 km
- Manila Vito Cruz Station 12-min drive; 2.9 miles/4.7 km
- Quirino Avenue LRT Station 13-min walk; 0.7 miles/1.1 km
- Vito Cruz LRT Station 21-min walk; 1 miles/1.7 km
- Pedro Gil LRT Station 23-min walk; 1.1 miles/1.8 km
- Self parking (surcharge)
- Airport shuttle (surcharge)
Aloha Hotel, Manila
Hotel Info: 866-539-8117from $42
- Bay side
- City Side
Also popular with travelers from the USA
At a glance
Key facts
Hotel size
- This hotel has 96 rooms
- This hotel is arranged over 6 floors
- Check-in time starts at 2 PM
- Check-out time is noon
Required at check in
- Credit card or cash deposit required
- Government-issued photo ID required
Travelling with others
- No pets or service animals allowed
- Free WiFi in public areas
- Free WiFi and wired Internet access in rooms
- Airport shuttle (available on request) *
- Self parking *
In the hotel
- Buffet breakfast daily (surcharge)
- 2 restaurants
- Coffee shop/café
- Room service (during limited hours)
- Spa services on site
- Business center
- Number of meeting rooms - 2
- Conference space
- Meeting rooms
- 24-hour front desk
- Dry cleaning/laundry service
- Hair salon
- Luggage storage
- Wedding services
- Number of buildings/towers - 1
- Roll-in shower
In the room
- Air conditioning
- Minibar
- Coffee/tea maker
- Iron/ironing board
- In-room massage available
- Private bathroom
- Shower/tub combination
- Free toiletries
- Cable TV channels
- Desk
- Free newspaper
- Free WiFi
- Phone
- Refrigerator
- Free bottled water
- Daily housekeeping
- In-room safe
Special features
DiningSze Chuan House - This restaurant specializes in Chinese cuisine and serves breakfast, lunch, and dinner.
Cafe Felisa - This restaurant serves breakfast, lunch, and dinner.
Aloha Hotel, Manila's small print
Also known as
- Aloha Hotel
- Aloha Hotel Manila
- Aloha Manila
- Hotel Aloha
Mandatory fees
Deposit: PHP 350 per night
Optional extras
Self parking costs PHP 30 per day
Rollaway beds are available for PHP 1000 per night
Buffet breakfast is offered for an extra charge of PHP 385 per person (approximately)
Cribs/Infant beds are available for PHP 500 per night
Airport shuttle service is offered for an extra charge of PHP 300 per vehicle (roundtrip)
We have included all charges provided to us by this hotel. However, charges can vary, for example, based on length of stay or the room you book.
Recent reviews
Good 3.4 /5 from 46 reviews
the staff r wonderful......will come back
Stay was ok. No real issues. I was just not ok with this ok
I stay here every time I'm in Manila. It's a great price, easy to get to from the airport (just say on the corner of Roxas Blvd. and Quirino, and every cab driver knows exactly where it is, ~175 Pesos if they use the meter, ~300 if they don't), has good AC, free wireless internet, and the people are friendly. And it's a short walk to Harrison mall (10 mins) or the Aristocrat restaurant (5 mins). A short cab ride to Mall of Asia. I've stayed here over 20 times. The bayside rooms face the water and have great sunset views, while the city side rooms are quieter. The only bad thing this time was that there were 2 different smoke detectors on my floor that had low batteries so they would beep every 30 seconds. ALL THE TIME. I stayed one night and told the front desk, but nothing happened. Then I went to the provinces for 1.5 weeks, then came back for the final 4 days, and they were still beeping! So I went and bought batteries and tried replacing them myself. Either the batteries I bought were equally dead, or the sensors themselves were broken because they just kept on beeping. 5 Days of beeping. Ugh. Luckily I always have earplugs, but it really did make the stay much harder. I heard beeps on other floors as well, so it's really something they should fix. Will I stay here again? Yup. Convenient.
great stay and great area with in walking distance to many places
I came to the Philippines and saw the hotel online the price was great i had a good view from my balcony rooms were very clean .The only thing that was wrong and it was minor was the shower head in our room was lose .But other wise a wonderful place to stay. as a matter of fact i am flying back very soon and going to stay there again
Is the description of this hotel not correct? Tell us


text:
I just wanted to tell you how much my husband enjoys your paint metals. He is painting his motorcycle as i write this. Thank you so much. I love your company.
More Testimonials »
Block Printing Starter Kit
Up to 30% Off
Deluxe Block Printing Kit
Up to 42% Off
Deluxe Screen Printing Kit
Inking Plate
From $7.85
Linoleum Cutter with Handle Assortments
Screen Printing Wood Frames
Super Value Opaque Screen Printing Kit


text:
Currently the full-time academic staff of the department includes 21 professors, coordinated by Prof. Dr. Frank Verboven. The research covers a wide range of areas in the broadly defined field of economics.
Researchers have built up considerable expertise in their fields, producing fundamental and policy-oriented research that finds an outlet in top general and specialized journals. The department also participates in many European, national and regional research projects. Professors are often called upon by national and international policy makers to give scientific advice. The Department of Economics attracts international guest professors as well as research assistants.
September 21, 2016
PhD defence by Jianbin Wu: Essays on financial volatitily modelling
More news
Public economics seminar by Subal Kumbahakar (Binghamton University)
Economics: Housing booms, fiscal myopia, and voter information by Alberto Solé-Ollé (University of Barcelona)
F. Vandenbroucke:
Managers helpen 250 arme gezinnen onder begeleiding van wetenschappers (Het Laatste Nieuws - September 15)
W. Moesen & P. De Grauwe:
Regering-Michel en EU moeten beslissen waar wordt bespaard en waar geïnvesteerd (Knack - September 14)
M. Goos:
Wordt uw verzekeraar straks een robot? (De Standaard - September 14)
More press articles
The Gaston Eyskens Chair was founded in 1975, on the occasion of the promotion of Prof. Dr. Gaston Eyskens to professor emeritus. This chair is intended to promote the teaching of theoretical and applied economics by organising bi-annually a series of lectures to be given by outstanding economists.
Copyright © KU Leuven | Comments on this page:
Faculty of Economics and Business
Production: Webmaster FEB | Most recent update: | Disclaimer


text:
Home > Sunglasses > Sting > SS4897 (8FTG)
SS 4897 is available in 4 additional attractive styles
New Releases
Size and availability
Free outbound and return shipping
30-day money-back guarantee
Low prices
Free outbound and return shipping
30-day money-back guarantee
Low prices
SS4897/8FTG Ophthalmic Glasses
These sunglasses complement your look with an accessory that is a cut above the rest. True to the Edel-Optics motto "SEE AND BE SEEN", you are on a par with celebrities, impressing in every environment. Would another colour not be a better match for your favourite outfit? Check also the other styles of SS4897in our 2015, and 2016 Sting range.
This Sting model is an all-rounder, equally suiting men and women. The metal frame is very light and comfortable to wear. It is both robust and classy. You can rely on the guaranteed UV400 protection, for all the sunglasses in our shop.
The next shipment is on its way, so we will have your Sting back in stock very soon. We hope the unbelievably low price will be a consolation for the short wait. If you buy at Edel-Optics you get the best price, because "in the sale" is our standard.
New Releases
Need help?
Don t hesite to call us
payment methods


text:
NEWSLETTERS Great Alibi stories, events and deals delivered to your inbox each week. No fooling!
Today's Events
Akin at Masley Hall
Amy Johnson and Sallie Scheufler present their distinct ways of examining themselves and their intimate relationships with photography.
Yoga en Español at South Broadway Cultural Center
Cerrillos Station Farmers Market at Cerrillos StationMore Recommended Events ››
Hero Quest • 10.7.2016


text:
Prosecutors want to fine 2 firefightersThe Associated Press | October 04,2013BENNINGTON - The state says it plans to fine two Bennington Rural firefighters for their part in lighting a camper on fire.
Both were at an annual "Mudder's day" mud-bogging event in May. In the finale, a camper was set on fire while a vehicle drove through it. It was seen on YouTube, where members of the department could be seen putting out the fire.
The firefighters, 28-year-old Ryan Nystrom and 35-year-old Roger Hughes, told the Bennington Banner they were the ones who set the fire, believing any permits necessary were in place.
They were among 15 firefighters who were temporarily suspended from the department because they didn't have the proper permits.MORE IN This Just InMONTPELIER After sitting on the Vermont Supreme Court for nearly 30 years, Justice John Dooley... Full StoryBRATTLEBORO Entergy Nuclear says it has found a potential source of water infiltration into... Full StoryMANCHESTER A little more than a week after it was shut down, the website for the town of... Full Story
- Most Popular
- Most Emailed


text:
Operating an automobile without proper proof of insurance.
I sure hope the police don't catch me driving dirty, my car insurance expired yesterday.
লিখেছেন- Tomorrowbot 29 de নভেম্বার de 2009
Trending Now
Can you define these popular missing words?
Top Definition
Driving with a concealed weapon, illegal substance, illegal narcotic, or drug paraphernalia
Trying to catch me driving dirty
লিখেছেন- da_skillitized_homie 19 de জুলাই de 2006


text:
On Saturday, August 24, 2013, Macy's will host its 8th annual Shop For A Cause benefiting charities nationwide.
* Exclusions and restrictions apply. See shopping pass for details. Savings passes are only valid in-store and not on macys.com.
Shopping passes are $5.00 and are available by emailing Kathie Lombardi at email@example.com for more info
Northeastern California and Northern Nevada chapter
Southern Florida chapter
Ohio, Kentucky & Indiana chapter
Middle Tennessee chapter
Stay in Touch!
Sign up to receive email from Make-A-Wish.


text:
Park Hotel
Madame Tussauds
The waxworks at Madame Tussauds are largely aimed at locals and cost a lot, but may make do for entertainment during one of Shànghǎi's...
Good Eighth Company Mural
A fine example of socialist realism sculpture, the Good Eighth Company Mural is in the plaza at People's Square metro exit near the...
People's Park
Occupying the site of the colonial racetrack (which became a holding camp during WWII), People's Park is a green refuge from Shànghǎi's...
Grand Cinema
This striking 1930s art-deco cinema was one of Shànghǎi's most famous movie houses, and today continues to screen the latest blockbusters.
Jiājiā Soup Dumplings
A fixture on Huanghe Road Food Street, this humble tiled restaurant is a real contender for one of Shànghǎi's best dumpling places, with...
170 West Nanjing Rd; 南京西路170号 · interesting places nearby
Park Hotel information
Designed by Hungarian architect Ladislaus Hudec and erected as a bank in 1934, the Park Hotel was Shànghǎi's tallest building until the 1980s, when shoulder-padded architects first started squinting hopefully in the direction of Pǔdōng. Back in the days when building height had a different meaning, it was said your hat would fall off if you looked at the roof.
Peruse the foyer for its art-deco overture. A short walk east along Nanjing Rd is the Pacific Hotel, formerly the China United Apartment Building, also equipped with some lovely lobby details.


text:
10 Free Flower Tutorials: How to Make Fabric Flowers, Flowers from Paper, and More
Bonus: Get our newsletter & special offers for free. We will not share or sell your email address. View our Privacy Policy
Thrifty Music Sheet Monogram
We are adding the project to your Holiday Projects.
The project was added to your Holiday Projects.
Click here to view your Holiday Projects.
Before you throw away that empty cereal box take a look at this tutorial and see what you can make out of that discarded cardboard! This Thrifty Music Sheet Monogram is so easy to make and would look great on top of your mantle or hanging on a wall. When it comes to recycle crafts, this one is one of the best because you don't need many supplies and the end result is a personalized piece of art that you can display in your home!
Estimated Cost: Under $10
Time to complete: In an evening
Primary Technique: Green Crafting
Difficulty Level: Easy
Find more projects and tutorials from craft designer Angie Holden.
Tags / Related Topics
Your Recently Viewed Projects
Images from other crafters
Love these projects? Get MORE delivered to your Inbox – FREE Click Here!
What's Hot
- 10 Mason Jar Crafts: DIY Home Decor and Handmade Gifts in a Jar free eBook
- Nature Crafts: 14 Things to Make With Pine Cones
- Soda Can Wind Chimes
- DIY Tote from a Tea Towel
- Refashioned Cardboard Storage Bins
- 47 Ways to Craft with Recycled Materials
- Bottle Cap Pinwheel Flower
- Thrifty Music Sheet Monogram
- How to Thrift Shop: 10 Thrifting Tips for Vintage Crafters
- Beautiful Upcycled Bulb Stars
Something worth saving?
- SAVE your favorite projects
- ADD personal reminder notes
- QUICKLY reference your projects
Free eBooks
- 10 Free Flower Tutorials: How to Make Fabric Flowers, Flowers from Paper, and More
- 10 Mason Jar Crafts: DIY Home Decor and Handmade Gifts in a Jar
- 10 Summer Craft Ideas and Garden Crafts for Summer
- DIY Wedding Ideas: 14 Wedding Crafts, DIY Wedding Gifts, and More
- Fun Easter Crafts: 9 Easter Decorating Ideas
- Handmade Wreaths for All Seasons: 14 Wreath Tutorials
- Happy Birthday to You: 8 Handmade Birthday Cards, Birthday Crafts, and Birthday Activities
- How to Make Easter Crafts: 7 Cute Easter Craft Projects
- Mother's Day Crafts: 9 Gift Ideas and Crafts for Mother's Day
- The Essential Guide to the Best Holiday Recipes & Crafts: 20 Ideas for a DIY Christmas, Thanksgiving, & Halloween
Our Newest Projects & Articles
- 26 Simple Crochet Patterns for Christmas: Festive Crochet Ideas
- Winter Flurry DIY Wreath Project
- Seasons Greetings Paper DIY Christmas Wreaths
- 200+ Easy Christmas Crafts for the Holidays
- "Go Green" for Halloween: 25 Halloween Recycling Crafts
- Amish Vintage DIY Hot Pad
- Gorgeous Amish Burlap-Wrapped Wheat
- Unexpectedly Brilliant Fall Wreath
- Woodland-Themed Winter DIY Coasters
- Christmas on the Frontier Bunting Template


text:
The average salary of a(n) Office Manager in Rockledge, Florida is $58,844.
The average hourly rate of a(n) Office Manager in Rockledge, Florida is $28.29.
The average bonus of a(n) Office Manager in Rockledge, Florida is $1,865.00.
The brevard Health Alliance Inc
This position reports to the Business Manager. Customer service and/or medical front office experience. Previous experience in a medical office or healthcare...View job on Indeed.com
Royal Oaks
This position assists the Manager with the day-to-day management of the facility's Business Office and may have supervisory responsibility in the Manager's...View job on Indeed.com
Paragon Radiology/Realty
Hours are 8 a.m. - 5 p.m. M - F with occasional after hours and weekend work. Established firm in Indialantic, FL seek qualified business manager for several...View job on Indeed.com
GV Melbourne
Superior 135 bed assisted living facility is seeking a BOM. Candidate must have long term care experience. Have advanced knowledge of accounts receivable andView job on Indeed.com
American Services Technology Inc.
Prepare and modify documents including correspondence, reports, drafts, memos and emails, schedule and coordinate meetings, for managers or supervisors, open,...View job on Indeed.com


text:
Category:Olivia Newton-John
|Authority control
Olivia Newton-John AO OBE (born 26 September 1948 in Cambridge, England) is a Australian singer and actress.
Media in category "Olivia Newton-John"
The following 38 files are in this category, out of 38 total.
- Olivia Newton John 2012.jpg 3.07 MB
- Olivia Newton-John 2.jpg 1.05 MB
- Olivia Newton-John.jpg 35 KB
- OliviaNewton-John10TIFF.jpg 479 KB


text:
1. Remove some filters or zoom out2. Save this search to get a heads up when new homes hit the market.
Popular homes near Whitestown
2 bd1 - 2 ba
apartment908 – 1082 sqftNot only do our apartments have all the comfort and style you want, but your rent also includes... More
1 - 3 bd1 - 2.5 ba
apartment1108 – 1843 sqftDeerfield Place is Utica's newest apartment and townhome community - now under construction!... More
3 bd1 ba
rental960 sqftBrand new 2016 Titan Home Model 947. Never been lived in before. This is a open concept home with... More
3 bd1 ba
rentalFirst floor of a 2 family home with garage and yard in the VVS school district. 3 bedroom, full... More
4 bd1 ba
apartmentSchool House Apartment building, Large apartments, pet friendly, month to month lease. 49d9698d.... More
5 bd4.5 ba
house3800 sqftif construction, space, character & setting are important to you this is the house! this country... More
3 bd1 ba
house1700 sqftRelax on the spacious deck overlooking the beautiful West Canada Creek, the Village of Newport... More
1 bd1 ba
rentalFreshly painted ONE bedroom unit located in North Utica. The apartment features 1 bedroom, living... More
4 bd1 ba
house1302 sqftHardwood Floors, City Water, Natural Gas Stove, Heat and Dryer. Excellent School District... More
2 bd1 ba
rental900 sqft900 sq ft /2 bedrm apartment located in "The Tontine" portion of the Oneida Community Mansion... More
3 bd1 ba
house1000 sqftNewly renovated 3- bedroom home . New propane boiler very energy efficient. Full storage in dry... More
1 bd1 ba
rentalONE bedroom unit located in North Utica. The apartment features 1 bedroom, living room, bathroom,... More
1 bd2 ba
rental1500 sqftThe Landmarc Building in downtown Utica has a beautiful Loft Sytle apartment available... More
1 bd1 ba
house1000 sqftNew 2 story 1 bedroom 1 bathroom Deck off second floor bedroom 8 x 12 deck Laundry hookup Plenty... More
3 bd1 ba
house4 Mohawk Acres Three-bedroom house; kitchen; dining room; living room; full basement with w/d;... More
Unfortunately nothing matches this search criteria. Try removing a filter or two
Or check out these popular homes
Summary for Utica, NY
Median rental price for rentals in Utica, NY for August was $1,100, an increase of 18% compared to the same period last year.


text:
Buy Colace Cod
Order no rx decadron.
Order no rx decadron find phenergan no rx required buy amantadine tenormin.
Find detrol online order no rx insect repellant patch order cheap bcaa, famvir online review order no rx nicotinell find menosan no rx required.
Buy colace cod order discount cholestoplex online find discount xenical dramamine overnight delivery.
Order carisoprodol.
Buy discount oral health dogs.
Discount cardizem cialis bodybuilding.
Oral health dogs online review find ponstel online buy soothenol no rx required, order cheap 3 panel drug test (thc/coc/opi) online buy cheap oral health dogs protein hair cream - extra nourishment sedural, find strattera no rx required.
Order ismo cod no rx midamor, arava imodium buy provera in us.
Buy hoodia weight loss gum no rx required, amoksibos.
Kytril free sample.
Shoot free delivery amantadine free sample buy discount atrovent find cheap bentyl online order cheap skelaxin online find neurontin no rx required order clonidine online, buy hip & joint chews small dogs cod tadalafil buy deluxe whitening system with plasma lamp, find lexapro online order imitrex cod find discount chromonexin online, lidin, buy kytril cod goji berry extract buy colace cod order discount dulcolax online runny nose find cheap betnovate online order tricor cod no rx alco screen 02 alcohol test order avapro cod find cheap aygestin, order chromonexin cod.
Order prevacid no rx required, discount celexa find cheap hip & joint chews large dogs online.
Order discount gluta-pep online corotenol find cheap skin & coat chews dogs, stress ulcers order no rx risperdal cheap vermox online buy cheap mexitil online.
Buy cheap cialis super active order evista buy no rx karela order biaxin cod, find discount zebeta ophthacare in canada order cheap glucophage online.
Buy no rx male enhancement oil, buy geriforte online, order cheap maxaman online, buy colace cod breast enhancement overnight delivery.
Weight gain formula find discount shuddha guggulu cheap geodon online buy immune support dogs no rx required buy tagamet no rx required order no rx female sexual oil buy no rx minocycline, find cheap stress gum online pepcid in us order cheap vein support online thyrox buy cheap inderal find discount avapro online, find cheap omnicef online order kytril cod buy cheap aciphex buy immune support cats online.
Free methox-400 find cheap viagra + cialis online buy discount trazodone rogaine.
Free tramaden, buy simplicef in us order provera no rx required guduchi.
Restrictive lung disease buy cheap styplon online, order zoloft no rx required order hip & joint support dogs online, order discount herbal testosterone online, free imdur actos.
Buy allegra no rx required.
Order discount hip & joint support dogs online, buy no rx breast augmentation find ultram no rx required lariam in us order no rx celadrin.
Weight loss order no rx rythmol sr.
Nuril sinequan free delivery lh ovulation strip test online review find discount cree-1200 prograf free sample order norvasc femara overnight delivery buy levitra professional altace in us, buy cheap sinequan ranolazine discount motilium, loxapine fairness cream order ashwagandha no rx required, herbal.
Female viagra buy energy patch hydroxyurea find calming chews large dogs online find nitrofurantoin no rx required 5-htp free delivery.
Order cheap prednisone online buy actoplus met online.
Find no rx breast sculptor, lioresal osteoporosis prevention.
Coral calcium overnight delivery.
Find metoclopramide discount tetracycline online find cheap atacand online.
Order orlistat, order voltaren online, norgestrel kre-alkalyn power free delivery, order atrovent no rx required, buy gaba (hgh booster) find endep cod order omega 3-1 cod, buy no rx lipothin discount azulfidine online, buy nitrofurantoin cod betamethasone.
Order cheap buprenorphine cassette test, find no rx urispas, detrol in canada gaba (hgh booster) in us, order no rx norvasc daily best dogs overnight delivery.
Cheap vibramycin online buy albendazole buy ditropan online.
Energy patch in us find cheap hip & joint chews small dogs online, myambutol overnight delivery, find discount pain relief patch online buy alli buy breast enhancement gum no rx required buy metformin online pancreatic insufficiency hay fever, order cheap shuddha guggulu.
No rx etodolac fexofenadin find cheap precose online.
Find evista.
Fortecortin find ayurslim cod microzide.
Find antivert online.
Avandamet overnight delivery, seroplex buy cheap levothroid online find benemid online buy phosphatidylserine online order daily best chews cats, find didronel online free cyklokapron order discount 5 panel drug test with adulteration test and e-z split cup, find discount accupril.
Calcium carbonate online review order dramamine cod find kytril online, buy breast enhancement gel cod, buy robaxin no rx required.
Lasix find discount nicocease, ranbaxy.
Find discount singulair online.
Free lh ovulation midstream test, buy cheap zma-power serum sickness quick relief cats in canada, discount hair loss cream order amino mass cod.
Order combivent no rx required, hydrochlorothiazide free delivery buy l-arginine patch online.
No rx diovan find discount 5 panel drug test with adulteration test and e-z split cup buy cheap penis extender standard online.
Buy famvir cod.


text:
Bariatric surgery requires making significant changes to your diet and lifestyle. Because you will be eating less food after your surgery, it's important to choose foods that will provide the nutrients you need for healing and overall health. Protein is particularly important for bariatric surgery patients. After surgery, you may find you have difficulty digesting common protein-rich foods such as meat or chicken and may need to seek out different sources of protein.
The Importance of Protein
Immediately following surgery, consuming adequate amounts of protein can help promote healing, prevent infection and keep you from losing muscle mass as you lose weight. Protein is also needed for hair, bone and nail growth. A lack of protein in your diet can lead to hair loss, brittle nails, fluid retention and a weakened immune system. Protein also has the most dramatic effect on satiety, according to Joan Salge Blake, author of "Nutrition & You," so eating high-protein meals and snacks can help keep you feeling full longer.
Protein Requirements
Immediately following surgery, you will be on a liquid diet, but you should still aim for 60 g of protein a day. Good sources of protein during this period include skim or 1% milk, Greek yogurt (plain, lemon or vanilla), small curd cottage cheese, diet pudding. As you begin to eat more solid foods, gradually work your way up to approximately 80 to 100 g of protein a day. According to the UCLA Metabolic & Bariatric Surgery Program, your individual protein goal should be 1.1 to 1.5 g per 2.2 lbs. of your ideal body weight. For example, if your ideal body weight is 150 lbs., your daily protein requirements would be 75 to 102 g. Keep a daily food journal to make sure that you are consuming enough protein.
Protein Sources
As you progress to puree or semi-soft and solid foods be sure to include a good source of protein in every meal and choose protein-rich foods as snacks. Always eat the protein first to make sure you don't get too full from other foods. Try to get most of your protein from whole food sources, such as chicken, seafood and low-fat dairy products. Good sources of protein include Greek yogurt, cottage cheese, beans, hummus and certain vegetables, such as broccoli and spinach. Try snacking on lump crab meat, string cheese or low-sodium deli meat. You may need to experiment to find out which protein sources work best for you. For example, some bariatric patients can tolerate hard-boiled eggs, but not scrambled eggs.
Protein Shakes
Bariatric patients often rely on protein shakes to meet their daily protein needs. If you consume protein shakes, choose products that are high in protein and low in sugar. Also keep your protein to carbohydrate ratio to no more than 3:1. For example, no more than 5 g of carbohydrate in a protein shake that contains 15 g of protein.
UCLA Metabolic & Bariatric Surgery Program: Post Bariatric Surgery Guidelines


text:
hazardous materials Companies in Wisconsin
Current filtering criteriaYou can remove a filter by clicking on the x
Compliance Communications, LLC
based in Milwaukee, WISCONSIN (USA)
Compliance Communications, LLC, a provider of Material Safety Data Sheet Compliance software is pleased to introduce the MSDS Administrator and MSDS Administrator Plus Hazard Compliance programs. These programs for your PC offer a cost-effective method for managing your MSDS sheets by scanning, indexing, and retrieving important MSDS information electronically.Hazard ...
Legend Technical Services, Inc.
Office in WISCONSIN (USA)
Legend Technical Services, Inc. is a diversified environmental analytical and consulting firm, operating a network of offices and laboratories throughout the midwestern and southwestern United States. We offer a broad range of standard and customized laboratory testing and consulting services to our clients whose needs are as diverse as our capabilities. All of our ...
Justrite Mfg. Co., L.L.C.
Distributor in WISCONSIN (USA)
Justrite is the leading source of storage, handling and security products including fire prevention safety equipment for hazardous materials, environmental protection spill containment devices, and specialized storage products. Since 1906 customers have looked to Justrite for innovative solutions to help protect workers, reduce fire risks, and improve productivity. ...
Hazardous Waste Experts
based in Fitchburg, WISCONSIN (USA)
Hazardous Waste Experts provides a wide variety of services for the management, transportation, and disposal of hazardous and regulated waste.We handle chemical waste, hazardous materials, toxic waste, medical waste, environmental remediation projects, contaminated soil, and other regulated materials.
DustMASTER Enviro Systems
based in Pewaukee, WISCONSIN (USA)
DustMASTER Enviro Systems is dedicated to the management of hazardous and non-hazardous dusts worldwide. As a division of Mixer Systems, Inc., DustMASTER utilizes their expertise in the design and manufacture of material handling equipment, batch plants and mixers for environmental and industrial markets.
Vector Technologies Ltd.
based in Milwaukee, WISCONSIN (USA)
Vector Technologies Ltd. and its Vacuum Engineering Division are leading world designers and manufacturers of powerful industrial vacuum cleaner and cleaning systems, air and hydro vacuum excavators, combination vacuum & jetters, hydro blast liquid recovery equipment, abrasive blasting and surface preparation equipment, portable vacuum trailer and baghouses, spill ...
Clean Air And Water Systems, LLC (CAAWS)
based in Dousman, WISCONSIN (USA)
"CAAWS" is a leading supplier and installer of geosynthetics. With offices in Wisconsin and Nebraska, CAAWS is able to provide turnkey containment solutions throughout the Midwest. The types of projects CAAWS is routinely involved with ranges from highly sensitive environmental applications such as hazardous waste landfills to more simple containment projects such as ...
Environmental Audits Inc.
based in West Allis, WISCONSIN (USA)
Environmental Rudits will meet or surpass your goals and objectives for your environmental health and safety programs.Our training will focus on decreasing lost time injuries and increasing productivity.Our assistance can range from as simple as workplace audits and hazard assessments, to full time implementation of your EH&S program.Our Goal will be to function as an ...
Regulatory Management Solutions
based in Milwaukee, WISCONSIN (USA)
Regulatory Management Solutions is a consulting practice with experience in the following areas: MSDS Authoring and Hazard Determination, Product Regulatory Review and Stewardship, HAZMAT (DOT/IMO-IMDG/IATA/ADR- Europe), Export/Import Regulations, FDA/USDA/TSCA/DEA Requirements, Due Diligence Awareness Training, Risk and Impact Analysis, Regulatory Process Development.
Eagle Eye Power Solutions
based in Milwaukee, WISCONSIN (USA)
Eagle Eye Power Solutions provides battery monitoring, testing, and charging solutions to power utilities worldwide. Substation backup battery maintenance is one of the most critical aspects to ensuring power to consumers when there is an outage or a system failure. Without proper battery testing and preventative maintenance, the Bulk Electric System (BES) is compromised ...
Ursus Remediation Testing & Technologies, LLC
based in Mount Horeb,, WISCONSIN (USA)
Ursus Remediation Testing & Technologies, LLC specializes in providing environmental engineers and professionals unique services in the environmental testing and remediation field. Ursus specializes in testing proprietary and non-proprietary chemistries at heavy metal and organic contaminated sites.Ursus conducts bench-level studies to determine the most effective ...
Cretex Specialty Products
based in Waukesha, WISCONSIN (USA)
Cretex Specialty Products, for over 26 years, the leader in solving manhole inflow and infiltration, has supplied the highest quality products to our customers. Infiltration/Inflow Control, Joints & Jointing Materials, Manhole Rehabilitation, Pipe, Wall Seals, Pipe/Sewer Rehabilitation, Sealants.
Badger State Drilling Co., Inc. (BSD)
based in Stoughton, WISCONSIN (USA)
Badger State Drilling Co., Inc. (BSD) has worked on both public and private hazardous waste sites throughout the Midwest including the states of Wisconsin, Michigan, Indiana, and Illinois. We work at safety levels "D", "C", and "B". Our crews have been through specialized on-site safety training as well as in-house training for driver safety, drilling practices and ...
K-Sun Corporation
based in Somerset, WISCONSIN (USA)
K-Sun Corporation, is a leader in labeling systems including label printers, software and supplies. K-Sun's exclusive stand-alone and PC-compatible electronic label printers are used in laboratory, hospital, industrial, manufacturing, research, safety, law enforcement, wire/cabling, engineering, construction, utilities, energy, transportation and other industries plus ...
based in Suring, WISCONSIN (USA)
we are a custom trailer builder, specializing in but not limited to the logging, refuse & material handling industries. Throughout the years we have designed & patented various aluminum products. Aluminum stakes, headboards & steel bunks are available for the logging industry. Freight haulers can utilize our aluminum stakes & bunks on flat bed trailers. Recently, we ...
Babcock & Wilcox MEGTEC
based in De Pere, WISCONSIN (USA)
Babcock & Wilcox MEGTEC provides environmental compliance solutions that make economic sense. B&W MEGTEC offers a portfolio of proven, high-efficiency technologies including wet electrostatic precipitators, scrubbers and liquid atomization systems for particulate and acid gas control, DeNOx and evaporative gas cooling, regenerative thermal oxidizers (RTOs) for VOC ...
Safety Helpers LLC
Office in Milwaukee, WISCONSIN (USA)
Safety Helpers L.L.C. is an Occupational Safety Training Company, Headquartered in Denver, Colorado. HAZWOPER training experts. We provide OSHA compliance training, DOT HAZMAT compliance training, EPA RCRA training, and various OSHA standard module topics. Our OSHA-authorized trainers are experts in their various fields. We hold regular scheduled Instructor-led safety ...
Weir Minerals Division
based in Madison, WISCONSIN (USA)
Weir Minerals` reputation is based on engineering excellence applied to innovative, customer-focused solutions for processing minerals and aggressive materials. Weir Minerals are specialists in delivering and supporting slurry equipment solutions including pumps, hydrocyclones, valves and wear resistant linings for global mining and mineral processing, the power sector ...
Schwing Bioset, Inc.
based in Somerset, WISCONSIN (USA)
Schwing Bioset is a team of engineering professionals dedicated to serving the wastewater, mining, and industrial markets with high performance products and engineered solutions. Our products are presently installed in a wide variety of custom engineered and highly specialized applications including wastewater treatment plants, mines, power plants, dredging, refineries, ...
Anguil Environmental Systems, Inc.
based in Milwaukee, WISCONSIN (USA)
Anguil Environmental Systems is a global provider of industrial air pollution control, emission abatement and energy recovery systems. We design, manufacturer, service and install thermal and catalytic oxidation technologies as well as heat recovery systems for a variety of applications and industries. Anguil Aqua was developed to help our industrial clients with water ...
Related Terms for hazardous materials
Need help finding the right suppliers?
Let the XPRTs do the work for you


text:
Second Pregnancy U.S. Edition
by Dr. Keith Eddleman, Dr. Joanne Stone
- #6412 in Nonfiction, Family & Relationships
- #7945 in Nonfiction, Health & Well Being, Health
Get eBook
- List Price$12.99
- Your price$10.69
Save $2.30 (18% off) and earn Kobo Super Points!
You'll see how many points you'll earn before checking out. We'll award them after completing your purchase.
Or, get it for 6000 Kobo Super Points!
See if you have enough points for this eBook. Sign in
Conceiving and carrying a second baby is not always easier or even as easy as a first pregnancy. During each stage of the process conception, pregnancy, labour and birth there will be experiences that differ. Your ability to conceive a healthy child and have a vaginal delivery, your risk of suffering certain pregnancy-related conditions, the ease with which you are able to maintain your physical wellbeing and that of your unborn baby while caring for an older child, and your being able to manage a two-child family are only some of the challenges you will meet in a second pregnancy.
People who read this also enjoyed
Ratings and Reviews
Overall rating
Be the first to rate and review this book!
You've already shared your review for this item. Thanks!
We are currently reviewing your submission. Thanks!
Second Pregnancy U.S. Edition
by Dr. Keith Eddleman,Dr. Joanne Stone
Share your thoughts
Tell readers what you thought by rating and reviewing this book.
Rate it *
Please make sure to choose a rating
Add a review* Required
Review *
How to write a great review
- Say what you liked best and least
- Describe the author's style
- Explain the rating you gave
- Use rude and profane language
- Include any personal information
- Mention spoilers or the book's price
- Recap the plot
(0) 50 characters minimum
The review must be at least 50 characters long.
Title *
The title should be at least 4 characters long.
Display Name *
Your display name should be at least 2 characters long.
Report a review
At Kobo, we try to ensure that published reviews do not contain rude or profane language, spoilers, or any of our reviewer's personal information.
Would you like us to take another look at this review?
You've successfully reported this review. We appreciate your feedback.
Second Pregnancy U.S. Edition
by Dr. Keith Eddleman,Dr. Joanne Stone
Thanks for Sharing!
You submitted the following rating and review. We'll publish them on our site once we've reviewed them.
by on October 01, 2016
eBook Details
- Carroll and Brown, January 2012
- Imprint:
Carroll and Brown
- 9781907952227
- Language:
- English
- Download options:
- EPUB 2 (DRM-Free)
You can read this item using any of the following Kobo apps and devices:


text:
Levitra is formulated as orange, round, film-coated tablets with "BAYER" cross debossed on one side and "2.5", "5", "10", and "20" on the other side corresponding to 2.5 mg, 5 mg, 10 mg, and 20 mg of vardenafil, respectively. In addition to the active ingredient, vardenafil HCl, each tablet contains microcrystalline cellulose, crospovidone, colloidal silicon dioxide, magnesium stearate, hypromellose, polyethylene glycol, titanium dioxide, yellow ferric oxide, and red ferric oxide.Orange-coloured egg-shaped tablet viagra soft tabs Onset of action: 16-45 minutes (effect NOT delayed by food)Benign Prostatic Hyperplasia
Urogenital — priapism [see Warnings and Precautions (5.3)]. The recommended starting dose of CIALIS for use as needed in most patients is 10 mg, taken prior to anticipated sexual activity.
Creatinine clearance 30 to 50 mL/min: A starting dose of 2.5 mg is recommended. An increase to 5 mg may be considered based on individual response. cialis for women IT industry: software and infrastructure. As more biologists aresome antibiotics including erythromycin and rifampicinGastro-oesophageal reflux
(N=94) Tadalafil 2.5 mgswelling of the face, lips or tongue which may lead to difficulty swallowing or breathing. where can i get viagra Hypersensitivity Reactions
A heart tracing (ECG).Inform patients that Levitra is contraindicated with regular and/or intermittent use of organic nitrates. Patients should be counseled that concomitant use of Levitra with nitrates could cause blood pressure to suddenly drop to an unsafe level, resulting in dizziness, syncope, or even heart attack or stroke.
Do not take Cialis more than one time each day. cialis 10 mg when to take Maintenance of Erection (SEP3)Frequency not reported: Steven-Johnson syndrome (SJS), toxic epidermal necrolysis(TEN), urticaria, herpes simplex, pruritus, sweating, skin ulcer, contact dermatitis, exfoliative dermatitis[Ref]Results in ED Population in US Trials — The 2 primary US efficacy and safety trials included a total of 402 men with erectile dysfunction, with a mean age of 59 years (range 27 to 87 years). The population was 78% White, 14% Black, 7% Hispanic, and 1% of other ethnicities, and included patients with ED of various severities, etiologies (organic, psychogenic, mixed), and with multiple co-morbid conditions, including diabetes mellitus, hypertension, and other cardiovascular disease. Most (>90%) patients reported ED of at least 1-year duration. Study A was conducted primarily in academic centers. Study B was conducted primarily in community-based urology practices. In each of these 2 trials, Cialis 20 mg showed clinically meaningful and statistically significant improvements in all 3 primary efficacy variables (see Table 11). The treatment effect of Cialis did not diminish over time.


text:
Bar Trays from CB2
Scroll tray, $14.95: Clean swoop of willow wood pops in bright matte orange with natural reveal at the edge. Seamless bentwood ends lend a hand (in white or orange).
Recycled tin tray, $59.95: International patchwork of recycled iron sheet packaging complete with logos, type, colors and patterns serves up entertainment/organization.
Two-tone lacquer tray, $29.95: Large red serving tray with grey exterior reflects hi-gloss lacquer over wood composite.
Small laquer tray, $14.95: Small grey valet tray with white exterior reflects hi-gloss lacquer over wood composite.
Format tray, $34.95: Crystal clear acrylic tray


text:
BiographyAriana Lewis is the Help Desk and ResNet Supervisor within the Division of Information Technology Services at Loyola University Chicago. In this role, Ariana works with staff and student workers in providing a high-level of customer service and technical support to both University members and the greater Chicago community. Additionally, she has a dual responsibility as the technical lead and project administrator in the project planning, execution, close out and maintenance of the Division's Call Tracking system. The responsibility includes maintaining the server and application requirements, application resource availability, and providing end-user training.
Due to having a passion for higher education access and mentoring both staff and students, she has recently become involved in Loyola University Chicago's staff mentoring program; allowing for the opportunity to work with newly hired employees in a professional development capacity.
Ariana's background includes the completion of a Project Management Certificate program, the acquisition of a Bachelor of Arts in Sociology from DePaul University and a Master's in Education focusing on Administration and curriculum development from Loyola University Chicago.
For additional information regarding Ariana's professional and academic experience, please visit her LinkedIn site: https://www.linkedin.com/in/arianalewis13
EDUCAUSE Presentations
EDUCAUSE Involvement
EDUCAUSE Connect 2015 Committee
Connect 3 Proposal Reviewers
Connect 2 Proposal Reviewers


text:
Princess Tutu fanfic chapter 3
Thanks for the review, dbzgtfan2004! I really appreciate it!
IMPORTANT: I skipped episode 7 because they know it's Rue who is Kraehe because they are identical in personality and looks. As in the last episode, seven, Mytho isn't around Duck because of Fakir.
Now on with the story!
Duck's POV
I search around for Mytho all over the town, with no luck. Where could he be? I walk back to the academy and throw pebbles at his window. Again, I have no luck and I begin walking away with my head bowed.
Fakir's POV
I hear tapping on the window and pear out through a small break in the curtains. It's that girl who transformed into Princess Tutu, Duck I think it was. After a while, she gives up and walks away with her head bowed.
"Persistent one isn't she?" I mumble as I watch her walk away. I hear my name being called and I turn around to see Mytho looking at me.
"Where's Tutu?" He asks
"Don't worry, she's not around anymore. What happened?"
"Tutu tried to help me restore a chard of my heart. Then Kraehe came and took that shard. Tell me, Fakir, would it be better if I didn't regain my heart?" He asks me.
"Forget about it, just go back to sleep." I say while leaning him back down on the bed.
"But" He tried to say
"Look, I brought you back here because I thought it'd be safer with people around. Forget about your worries and sleep." I say. He nods his head and obediently closes his eyes.
Rue's POV
I toss and turn in my bed then stare at the box glowing on my dresser. I stole Mytho's heart shard last night
"Why did you bring me back with you?" The shard said.
"I brought you here because you will be serving me a very important purpose in the future, my shard." I say while putting my hand on its face.
"What purpose is that, Kraehe? I must know for I am the desire for knowledge."
"That is for me to know and you and everybody else to find out in the future." I say with a smirk.
Duck's POV
I walk down the hall to my room with my head bowed. I wonder where Mytho could be.
"I saw you!" I hear Pike say as she pokes her head through her door.
"I saw you too!" I hear Lilie say as she does the same.
The two began tackling me down the hall into my room. They knock me down into my room's floor right before the stairs to walk up into my sleeping area and land on me.
"Confessing your love at the window?! Duck, you're so bold! Which one did you confess to, or was it both?! You've got to tell us, we're dying here!" Pike exclaims excitedly.
"It's like a reverse Romeo and Juliet, except there are two boys and you're sure to get rejected by both because it's a one-sided love!" Lilie chirps.
"That's not what I was doing, Mytho disappeared and I wanted to make sure he was ok!" I defend while sitting up.
"That's right, I forgot about that, but it does hurt that Fakir is in the same room nursing him to heal, as always!" Pike says while giving me a suggestive nudge.
"You just couldn't contain your love and confess, right?" Lilie says in a dreamy tone
"No it's not like that!" I whine. When will these two ever listen to me?
"Take this chance to attack!" Pike says while shoving me out of the door and leads me out of the dorm area.
"You have absolutely one hundred percent no hope of having Mytho because of the wonderful Rue." Lilie chirps.
"I keep telling you guys, it's not like that!" I yell
"Go for it and take your losses!" Pike says
"Lose and be crushed! I'll be there to catch you when you fall into the pits of depression!" Lilie says joyfully.
"But I was just-" I try to say
"He might just show up to class today!" Pike says
"You think so?" I ask
"You're so hopeful, it's so cute! I can't wait until you get rejected, you'll be even cuter!" Lilie beams. How can she be so perkily sadistic?
We get to the academy and hid behind some bushes.
"Do you really think he'll come today?" I ask
"If he does, you'll have to say it properly and clearly, okay?" Pike says.
"Say what?" I ask.
"Oh come on Duck, what else besides your love for them!" They chorus with sparkling eyes.
"What, I can't tell him that! Because…" I begin to say. If I tell him that I love him, who I'm not even fully sure if I fully do love, I'll disappear.
"Oh you coward!" Lilie says. She gives me a shove and I go tumbling down the small hill behind us and land on my stomach. She exclaims 'how did this happen?' and Pike smacks her on the back of the head saying that it's her fault, which it is.
I'm still momentarily dizzy until I hear a voice call me name. I look up to see Mytho looking at me. He asks me what I'm doing on the floor and I stupidly just say his name. I get up and grab his hand exclaiming that I'm glad he's alright.
After hearing two shrieks in the distance from my friends I realize that I just held his hand. I let go in haste and apologize.
"I'm so sorry!" I said while spinning away from him. "I looked all over town and I couldn't find you, so I thought something happened to you and I was so relieved to see that you were okay that I held your hand! I'm sorry!"
"You were looking for me?" He says with a confused faces.
"Uh, yeah, oh!" I cover my mouth. I forgot he doesn't know that I'm princess Tutu, only Fakir does!
"Do you know about Tutu?" He asks.
"Um, well" I start off, but I hear Fakir's voice.
"What are you two doing?" He says I look up to see Fakir jumping cleanly over the ledge on the second floor and land smoothly.
"Fakir," I say in shock.
"Mytho, go ahead, I'll be right there." Fakir ordered. Mytho gives him a nod and begins to walk away.
"You," He says
"Yes?" I yelp
"You're such an eyesore, Tutu" He says while walking next to me "Listen up carefully, stay away from Mytho. If you do, you'll regret it."
With that, he walks away, leaving my frightened to the point I drop to my knees. I know Fakir is just trying to help Mytho, but why does he have to be so freaking frightening?!
I give a sigh and walk back over to my friends. Lilie happily says it was a failure and Pike just pats my back and tells me they'll send a revenge match for me. What the heck is up with these girls and revenge?
XXxxxX…. Time skips …xXxxxX
I hear the bell ring in the distance and sigh. I just skipped all of my morning classes thinking of that look in Fakir's eyes. I can tell they held a lot of compassion for Mytho, but he always glares at people, shunning them away all from Mytho's sake. Fakir really is a knight, but he won't the other soldiers in this battle help him. He is pretty chivalrous, minus the part about being kind to women, but that's because they are the ones who bother Mytho the most.
I hear a familiar tune and look up to see green hair poking out behind a tree. I run over to see Ms. Edel with her tune box.
"Ms. Adel I need some advice." I say with a sigh. "Fakir is pretty intimidating and I want help them, but I can't seem to get near him!"
She shows me a gem that is split in half and tells me the value of team effort. I think I have an idea. I might find a way to make him like me if I force him to get to know me! Now that I think of it, it kind of annoys me that he immediately discarded me, I guess he'll be the one friend that you'll randomly need to hit for past occurrences.
"What is Fakir to you, duck?" Ms. Adel asks me.
"Someone I want to like me." I reply.
"For what reasons do you want him to like you?"
"Because I want to be his friend and ally," I answer all too quickly
"What is Mytho to you?"
"A friend, a friend I want to help protect" I reply with wide eyes. For once, I haven't referenced Mytho as the guy I'm in love with.
"Then do what you must to protect the ones close to you." Ms. Adel says. I blink my eyes for a second and she magically disappears. Seriously, she needs to teach me how to do that.
I let a sigh out of my lips and stand up. I begin running towards the school. I come to the class that I skipped and burst in through the doors. All eyes are on me and I take a breath.
"Mr. Cat," I exclaim without much breath, "Ms. Goat says she is on her way to accept her offer to marry you! She said a lot of things that I can't repeat, and I'm a teenager! Hurry while you can, I think you'd prefer to remain a cat with fur, not one of those creepy hairless cats; I'll cover for you!"
Mr. Cat scurries to collect his things and before he leaves he engulfs me in a tight hug and says he is giving me extra credit for saving his life. Then, he sprints out of the class like a dog was chasing him.
I drop my façade of being extremely out of breath and in a panic with a slight flip of my head to get my bangs out of the way of my now glaring blue eyes. Once I see Mr. Cat run out of the school gates with, surprisingly, Ms. Goat running after him surprisingly fast I decide to speak. (A/N: Sorry I forgot if it was a goat or a sloth.)
"Fakir," I say, promptly gaining his attention, "you're coming with me. I have a problem with you."
"And what problem would that be, Duck?" He inquires with his arms crossed and an eyebrow arched.
"You, my enemy-like friend are a jerk." I say while poking his chest with every word. "I don't want to embarrass you by telling you off in a few different languages in front of the whole class."
This seemed to surprise everyone (except my two cheering friends), especially Fakir. To my surprise, he agrees to come with me in front of the school gates in thirty minutes. I nod in compliance and storm out just as I entered the room.
As soon as I am out of the room and down the hall a smirk graced my lips. Screw being a ballerina, I can be an actress!Fakir's POV
Mid way through a lecture about love, Mr. Cat is (thankfully) interrupted by Duck bursting through the doors of the classroom. For once, I'm happy to see Duck; she just saved me from being bored to death.
"Mr. Cat," Duck exclaims without a breath in her lungs, "Ms. Goat says she is on her way to accept her offer to marry you! She said a lot of things that I can't repeat, and I'm a teenager! Hurry while you can, I think you'd prefer to remain a cat with fur, not one of those creepy hairless cats; I'll cover for you!"
Mr. Cat scurries to collect his things and before he leaves he engulfs her in a tight hug and says he is giving her extra credit for saving his life. Dang, is he grateful or what? He sprints out of the class like a dog was chasing him.
As soon as Mr. Cat is out of the gates, Duck's whole demeanor is changed. With a flip of her red hair, her eyes are revealed in very shockingly scary glare.
"Fakir," She says with a very pissed off look in her eyes, "you're coming with me. I have a problem with you."
"And what problem would that be, Duck?" Inquires with my arms crossed and an eyebrow arched. Even though this girl is pretty feisty looking right now, no way am I dropping my façade.
"You, my enemy-like friend are a jerk." She says while poking my chest with every word. "I don't want to embarrass you by telling you off in a few different languages in front of the whole class."
I nod my head and tell her I'll meet her in front of the school gates in a half hour. If she's this pissed off it must be important. Then again, why should I care? I don't hang out with her or even work with her to save Mytho.Time skip, Duck's POV
I stand in front of the gate in one of my casual outfits. It's a simple outfit with jean shorts that end a little more than half way to my knees, a pink shoulder shirt that fits me in a fitting baggy way with a black ribbon tied around the bottom of my shirt just in case it flies up with a black wife beater t-shirt under the shoulder shirt, and black ballet flats. My hair is in its usual braid down my back.
I see Fakir walking towards me exactly on time too. He's wearing jeans, a fitting black shirt that lightly shows his abs, a jean jacket, and simple but taken care of black shoes.
I send Fakir a dirty look and gesture him to follow me. I like acting; this is really fun seeing him semi-freaked out! After a while of walking in a thick silence, I fall back next to Fakir with a scowl on my face.
"Excuse me young lady," I hear a voice to right say. I turn my head to see a man at least six years older than me with a rose in his hand.
"Yes?" I respond, unsure on what to say.
"Would you like to accompany me today?" He asks while basically shoving the red rose into my hand. "It's obvious your boyfriend here doesn't know how to treat a lady seeing how such a beauty as yourself has a scowl upon your perfect pink lips."
A light blush rose to my face, I'm not as pretty as he thinks I am.
"Ah, that pink hue on your face is much better my lady." He says while putting an arm around my waist and bringing me close to his side. I let the obvious discomfort arise to me face. I try inching away from him to no avail.
"Let go of her." Fakir says with a glare.
"Why?" The strange man asks. "You obviously weren't getting anywhere with her, I got a response from her that wasn't anger."
I feel myself being pulled from the man's grip and into Fakirs. He holds my waist lightly and tilts my chin towards him. My face instantly glows red. I stutter his name under my breath.
"If my eyes don't deceive me, that's a response that isn't anger. I think she's awestruck if anything. My grip is loose enough to get out of easily if she wanted to get away from me." Fakir says with a smirk. "You trapped her. Come on let's go."
With that, Fakir leads me away with his hands locked in mine. He whispers for me to rest my head on his shoulder because the man is still looking and he may stalk me if I don't as he puts his hand on my waist. I wrap my arms around his torso and rest my head on the crook of his neck.
We stay this way for a while until the man stops following his. I let go of Fakir as he lets go of me and I instantly miss the warmth as odd as that sounds.
"Thanks for saving me, Fakir." I say as we pass the pond.
"It's no big deal." He said while looking away from me.
"Ya know if the girls at school you were so knightly they'd be all over you." I tease. "You'd be much more popular than Mytho with your bad-ass style too. I think you'd make some girl very lucky one day."
"S-Shut up," He stutters with a light blush on his face, "w-what did you want to talk to me about that you were so mad at me all this time, not that I care, but I'm a bit curios."
"Oh I was never actually mad at you." I say while waving off the thought as I come towards the white gazebo I sat under earlier. "I just used that as an excuse to bail you out of class."
"Well you're one hell of an actress then, maybe you should transfer to the drama department." He says with a slight smirk. "Why did you get me out of class or did you just want to have a challenge?"
"I wanted to get to know you." I say with a shrug. "You know I'm Tutu and I know you're the knight. I want to work together to collect the shards of Mytho's heart. I'm not trying to gain all of his heart shards to take him over or something. I personally want to help him as a friend and get it over with so I don't have to have dance battles with Kraehe who uses needle like crow feathers. Those things hurt! If you just listen to me and become my ally I won't annoy you into doing as I want."
"Aren't you already doing that?" He mumbles.
"No I'm not, actually." I reply. "I can be quiet persuasive. If you don't want me to use my trump cards than just agree that you'll be my ally. Besides, you just saved me, so we're already friends."
"That doesn't mean anything." He says stubbornly. "I'll agree to your proposition if you tell me how you are able to transform into Tutu."
"That's easy; my necklace transforms me when a heart's shard is near." I reply while pointing to my necklace.
"That's it?" He asks.
"No." I reply. "There's a story behind this I don't even know, and I'm in it!"
And just like that, Fakir became my ally. The rest of the afternoon is spent on me bent on getting Fakir to laugh for more than three seconds (an epic fail, just to let you know).
I hope you like this chapter! Sorry for not updating in so long!


text:
This is a split board - You can return to the Split List for other boards.
The fundamentals for genetics are how traits are passed via chromosomes. AND remember the logo for Japanese X and Y with the double helix?AND what Pokemon is Mewtwo? That's right, the genetic Pokemon.
so the plot thickens, either way im happy that this is more than likely mewtwo. Ive liked the design since it was first leaked.
oh well.. I hoped it's a new pokemon but I guess I was wrong..
You are not allowed to request a sticky.


text:
Welcome to Hillcrest Jewish Center
A landmark Conservative synagogue serving Queens, Hillcrest seeks to foster a love of Judaism and inspire acts of "Tikkun Olam" -- making the world a better place.
Rabbi Manes Kogan on this week Parsha.
Click to share this video with your friends.
You are invited to get to know us through this video glimpse into our warm, welcoming community with Rabbi Manes Kogan and members of Hillcrest Jewish Center.
Featured Events
Featuring guest speaker
Dr. Nancy Foner
Distinguished Professor of Sociology at Hunter College and the Graduate Center of the City University of New York.
Topic: "Fear, Anxiety, and Immigration."
Adult Education
Featured Guest Speaker
Dr. Joseph Benatov
Hebrew Lecturer in Foreign Languages at
University of Pennsylvania
Topic: The Sephardic Balkan Communities and The Holocaust
Sisterhood 77th Anniversary Celebration Brunch
Historian Marty Schneit will be taking us along Old Route 17
to the Catskills for a nostalgic visit to the hotels and
look back at the great comedians of "The Borschet Belt."
$50 per person; Sponsors: $60 per person
Please RSVP no later than Thursday, October 20, 2016
Mini Bazaar
Beautiful items for you to choose from for all your gift-giving needs
An Evening Celebrating
Friday, November 18, 2016 at 6:00 PM
The Kogan Family's 10th Anniversary, and
Cantor Moti & Lesley Fuchs' 18th Anniversary at Hillcrest
Kabbalat Shabbat and Marriv at 6:00 pm
Followed by a Family Friendly Shabbat Dinner
$45 per adult, $18 per child ages 4-16, children under 3 are free.


text:
List of nicknames for Chicago
From Wikipedia, the free encyclopedia
|This article needs additional citations for verification. (May 2010) (Learn how and when to remove this template message)
This article lists nicknames for the city of Chicago, Illinois.
Minor nicknames[edit]
- "Windy City" - Two theories for this nickname include the chilly gusts coming off Lake Michigan and the hot air bellowing from politicians.
- "Beirut by the Lake" – From a Wall Street Journal article during the Council Wars of the 1980s. The nickname is a reference to the Lebanese capital of Beirut, which – at the time – was a major center of conflict during the country's ongoing sectarian civil war.
- "Chi-city" – Used by Kanye West in the song "Homecoming" and Common in the song "Chi-City"
- "City by the Lake" – Used as early as the 1890s.[1]
- "City in a Garden" – English translation of the Latin motto on the city seal: "Urbs in Horto"
- "City on the Make" – From "Chicago, City on the Make" (1951), a prose poem by Nelson Algren
- "Great Commercial Tree" – From the State Anthem of Illinois
- "Heart of America" – Chicago is one of the largest transportation centers in America and its location is near the center of the United States.
- "My Kind of Town" – According to the song "My Kind of Town (Chicago Is)" (music by Jimmy Van Heusen, words by Sammy Cahn, 1964) popularized by Frank Sinatra. (Originally from the film, Robin and the Seven Hoods, about a fictional popular Chicago gangster).
- "Paris on the Prairie" – From the 1909 plan for the City of Chicago created by Daniel Burnham.
- "Sweet Home" – From the Robert Johnson song Sweet Home Chicago.
- "That Toddling Town" – According to the lyrics of the song "Chicago" (music and words by Fred Fisher, 1922) also popularized by Frank Sinatra (as well as Tony Bennett).
- "The Big Onion" – An homage to the original Native American name for the area (shikaakwa, which means "wild onion" in the Miami-Illinois language), in parallel with a popular New York nickname, "The Big Apple"
- "The Black City" – a reference to the pre-1893 World's Fair Chicago (which site was called "The White City"); the phrase was prominently used in such media as The Devil in the White City
- "The Chi" (pronounced "shy") – Short for the word 'Chicago'
- "The Chill or Chi Ill" – Also used by rap musicians from the area (Chill as in Chicago Illinois)
- "The City Beautiful" – A reference to the reform movement sparked by the World's Columbian Exposition,[2] used by Hawk Harrelson when the Chicago White Sox open a game at U.S. Cellular Field
- "The City That Works" – According to former Mayor Richard J. Daley
- "The Great State of Chicago" – Used ironically (by Chicagoans) and pejoratively (by the rest of Illinois). A reference to the great political, cultural, social and ideological divide between the metropolis that is Chicago and the rest of the mostly agricultural State of Illinois.
- "The Jewel of the Midwest" – Often used to describe Chicago and its various tourist destinations.
- "The Third Coast" – As a reference to its long Lake Michigan shoreline. In that vein, it is used to describe the city's draw on people relocating to Chicago. It is a play on the traditional idea that people are drawn from land-locked states to coastal states.
- "Chi-beria" – A play on Siberia, a nickname largely used during the 2014 North American Cold Wave.[3]
- "The Cogs" – A play on words used to describe its emphasis on heavy industry and manufacturing, or the "cogs within cogs" of the city's notoriously corrupt bureaucracy. Also a play on the second syllable of Chicago: 'cags.
- "Chi-raq" - A play on the name Chicago and Iraq, due to the high number of homicides that occur in the city.
See also[edit]
- List of city nicknames in Illinois
- Lists of nicknames – nickname list articles on Wikipedia
- ^ Seeger, Eugen. "Chicago, the Wonder City" (p. 384) G. Gregory Printing Company, 1893 – Chicago
- ^ Levy, John M. (2009) Contemporary Urban Planning.
- ^ Parker, Alex (January 5, 2014). "Chicago Extreme Cold: City Dubbed 'Chiberia' as Dangerous Weather Moves In", DNAinfo. Retrieved January 23, 2016.


text:
Alcohol misuse frequently leads to muscle weakness, which may also occur in the setting of acute and chronic alcoholic myopathies. At the cellular level, ethanol has been found to interfere with signalling mechanisms in cardiac myocytes, skeletal myotubes, and smooth muscle cells. In this study, we focused on the effects of ethanol on the intracellular calcium ([Ca2+]i) transients responsible for excitation–contraction (EC) coupling in isolated mouse skeletal fibres loaded with the fluorescent Ca2+ indicator fura-2. Following electrical stimulation, ethanol caused a significant reversible dose-dependent reduction in [Ca2+]i transient amplitude, already significant at 100 mM ethanol (P = 0.03), without modifying resting [Ca2+]i. Evaluating the potential loci for the effects of ethanol, we indirectly measured sarcolemmal Ca2+ entry by monitoring Mn2+-quenching of intracellular fura-2 via the nitrendipine-sensitive Ca2+ channels during electrical pacing. Ethanol at doses of 20 mM and greater caused a dose-dependent reduction in the rate of fura-2 quenching (all at P < 0.05). Moreover, the intracellular pool of Ca2+ releasable by caffeine was found to be reduced at a minimum of 300 mM ethanol (P = 0.05). We conclude that ethanol reduces the [Ca2+]i transients underlying EC coupling in single mouse skeletal muscle fibres. This acute effect of ethanol was primarily due to an inhibitory effect of ethanol on sarcolemmal Ca2+ influx via voltage-operated Ca2+-channels and, to a lesser extent, to a reduction in the Ca2+ sarcoplasmic reticulum loading state. This inhibitory effect of ethanol may be implicated in the development of muscle weakness with alcohol consumption.
The ergolytic effect of ethanol has long been recognized. Although acute and chronic myopathies are the most usual reasons for muscle weakness in alcoholics, acute intake of ethanol may lead to muscle weakness, without structural muscle damage (Urbano-Márquez et al., 1989; Fernández-Solà et al., 1996). Eichner (1993) observed that, on drinking alcohol, athletes exhibited a decrease in their muscle strength and contraction velocity, which was reversible after ethanol suppression. It is well known that calcium-dependent excitation– contraction (EC) coupling is the main mechanism responsible for skeletal muscle strength. Previous studies in other muscle specimens, such as rat cardiomyocytes (Thomas et al., 1989, 1996; Danziger et al., 1991; Nicolás et al., 1996), cultured myotubes (Cofán et al., 1995; Nicolás et al., 1998), and smooth muscle cells (Zhang et al., 1992), have demonstrated that acute exposure to ethanol interferes with EC coupling in a dose-dependent manner, mainly through a reduction in [Ca2+]i transients. However, no studies have evaluated the effect of ethanol on EC coupling in mammalian mature skeletal muscle fibres. Using fluorescence imaging techniques, we have measured the acute effects of this toxin on the pathways involved in EC coupling, a potential cellular mechanism for ethanol-induced muscle weakness. The results showed that ethanol depressed the [Ca2+]i transients underlying EC coupling in skeletal muscle fibres due, at least in part, to an inhibitory effect of ethanol on voltage-operated Ca2+-channel activity and a reduction in the calcium content at the sarcoplasmic reticulum.
Isolation of muscle fibres and loading
Intact fibres from the flexor digitorum brevis and interosseal muscles of CD-1 mice were isolated by a classical enzymatic dissociation process. Mice were killed by cervical dislocation, muscles were removed and incubated at 37°C for 90 min in Tyrode solution (140 mM NaCl, 5 mM KCl, 2.5 mM CaCl2, 1 mM MgCl2, 10 mM HEPES, pH 7.4 with NaOH) containing collagenase (2 mg/ml) (Sigma, St Louis, MI, USA). They were then rinsed with collagenase-free Tyrode solution and stored up to 6 h at 4°C until used. Intact single fibres were separated from the muscle mass by gentle trituration with the tip of a disposable pipette. Isolated fibres were loaded with 4 μM fura-2 acetoxymethyl ester (Molecular Probes, Eugene, OR, USA) in incubation buffer [121 mM NaCl, 4.7 mM KCl, 1.2 mM KH2PO4, 1.2 mM MgSO4, 1.25 mM CaCl2, 10 mM glucose, 5 mM NaHCO3, 10 mM HEPES, 0.01% bovine serum albumin (BSA), pH 7.4 with NaOH] for 25 min at 37°C, with gentle shaking.
Measurement of [Ca2+]i transients and contraction
After loading with fura-2, isolated fibres were washed with incubation buffer and placed on a laminin-coated coverslip in an open flow chamber (2 ml volume) mounted on the stage of a Diaphot-300 inverted epifluorescence microscope (Nikon, Tokyo, Japan). The stage, 20×-glycerine immersion objective (Nikon) and chamber were maintained at 17°C. The chamber was equipped with platinum electrodes to allow electrical field stimulation of the fibres within the field of view, which was applied at 0.25 Hz (5 ms duration) (A385, WPI, Sarasota, FL, USA). The electrical stimulator and excitation filter changer were synchronized with the imaging computer by a prepulse from an interval generator (A300, WPI). The cells were perfused continuously at a flow rate of 7 ml/min with modified incubation buffer containing 2 mM CaCl2.
The imaging system and techniques for obtaining digital images of cellular fluorescence at high-time resolution have been described in detail previously (O'Rourke et al., 1990; Nicolás et al., 1996). In brief, an electrically cooled charge-coupled device (CCD) camera (CH250, Photometrics, Tucson, AZ, USA) was used in a mode that allowed a consecutive series of images to be stored in the photon wells of the CCD chip. To achieve this, the CCD was masked, and the image of the cell was focused on one end of the detector. The image was shifted periodically along the detector, so that a series of subimages was accumulated on the CCD, digitized and stored as a single image in the memory of the imaging computer (MacIntosh 840AV, Apple Inc., Cupertino, CA, USA). To evaluate the contractile cycle of the fibres, series of 8- and 16-ms time-resolved fluorescence images were created by averaging four and two contractions, respectively. Fluorescence images were collected alternately at excitation wavelengths of 340 and 380 nm (10 nm bandwidth filters) to excite the Ca2+-bound and Ca2+-free forms of this ratiometric dye, respectively. The emission wavelength was 510 nm (120 nm bandwidth filter). In order to minimize photobleaching, a computer-controlled shutter was used to limit the exposure of the cells to excitation light. The individual contractions of most fibres were reproducible and image pairs co-registered accurately. [Ca2+]i values were calculated from the 340 to 380 nm ratios, as described previously (Grynkiewicz et al., 1985).
At the end of each experiment, fibres were exposed to butanedione monoxime (20 mM) to prevent fibre shrinkage, and then to digitonin (30 μg/ml) and ethylene glycol-bis (β-aminoethyl ether) N,N,N,N-tetraacetic acid (EGTA) (25 mM) for 10 min (Sigma). This treatment releases the intracellular fura-2 leaving the residual fluorescence at each wavelength due to fibre autofluorescence and any compartmentalized dye. The residual fluorescence was measured over the same region of the fibre as the Ca2+-dependent fluorescence. To ensure that a steady state was achieved, [Ca2+]i was obtained under control conditions and after 10 min incubation with ethanol (20–500 mM; 90–2300 mg/dl), and after a 10-min washout period. Studies with fluorescent dyes indicated a complete chamber buffer exchange within 1 min. Fibres were selected for study based on their overall physical appearance (elongated rods with well-defined sarcomere structure and no blebs), quiescence in the absence of stimuli, and their ability to contract in response to electrical stimulation. Because fibre size exceeded the field of study, only one edge of the fibre was focused and the contractile response was not analysed.
Measurement of sarcolemmal Ca2+channel activity using Mn2+-influx
A potential locus at which ethanol may interact with EC coupling in skeletal muscle cells is at the level of sarcolemmal ion channels. Muscle action potential and contraction are dependent on Ca2+ currents, and it is generally accepted that hormones or drugs that modulate the inward calcium current amplitude affect muscle contraction. To investigate the possible role of ethanol on voltage-dependent Ca2+ entry, we evaluated the effects of ethanol on sarcolemmal Mn2+-influx via the nitrendipine-sensitive Ca2+ channels by measuring the rate of fura-2 quenching by Mn2+ entry when electrical stimulation was applied. Isolated fibres preloaded with fura-2/AM (7 μM) were perfused at a flow rate of 7 ml/min with a modified incubation buffer (130 mM NaCl, 4.7 mM KCl, 0.1 mM KH2PO4, 1.2 mM MgSO4, 0.1 mM CaCl2, 10 mM glucose, and 10 mM HEPES, pH 7.4 with NaOH) at room temperature (22°C). After a perfusion period of 10 min in which electrical stimulation at 0.25 Hz (5 ms duration) was applied and a field of contractile fibres was selected, the system was switched to a modified incubation buffer which contained 0.2 mM MnCl2, and the electrical pacing was stopped. Fluorescence images were collected every 2 s using 360 nm excitation and 510 nm emission filters. After 100 s, during which the basal quench rate was determined, electrical stimulation (1 Hz, 100 ms duration) was initiated in order to activate Mn2+ entry via voltage-sensitive Ca2+ channels. The electrically stimulated Mn2+ quench rate was completely inhibited by nitrendipine (10 μM) (LC Laboratories, Woburn, MA, USA), indicating that it reflects the activity of L-type Ca2+ channels.
Measurements of caffeine releasable Ca2+pool
To evaluate the sarcoplasmic reticulum (SR) Ca2+ loading state, caffeine (15 mM), dissolved in modified incubation buffer without CaCl2 or BSA, was perfused directly onto one fibre for 10 s using a puffer micropipette (6-μm tip diameter) Narishige IM-300 (Tokyo, Japan) at a pressure of 6 psi. Fibres were perfused continuously at a flow rate of 7 ml/min with incubation buffer containing 2 mM CaCl2 at room temperature (22°C). Because the SR Ca2+ content is modified by the stimulation frequency, fibres were stimulated electrically (0.25 Hz, 5 ms), and caffeine was puffed onto the fibres in phase with the ongoing electrical pulse at the time point of the next stimulation pulse. Pairs of images were collected every 2 s, and fluorescence values at each time point were expressed as a ratio to the initial resting fibre fluorescence. This ratio was calibrated in terms of [Ca2+]i using the initial [Ca2+]i value obtained from the 340 to 380 nm fluorescence measurements, as described previously (Renard et al., 1994). Individual fibres acted as their own controls, with the response to caffeine being compared before and after treatment with ethanol.
Statistical analysis
Standard statistical methods from the SPSS Statistical Analysis System V6.1 (SPSS, Chicago, USA) were used. Paired and unpaired Student's t-tests were used to analyse differences. Correlation studies were obtained by Pearson's correlation coefficient and regression analysis. All variables are expressed as means ± SEM, and a level of probability (P) lower than 0.05 was judged as being significant.
Effect of ethanol on [Ca2+]itransients
We evaluated the influence of ethanol on electrically triggered Ca2+ transients in single skeletal fibres that showed repetitive Ca2+ mobilization in response to the electrical field stimulation. In control cells (n = 60), [Ca2+]i increased from a mean resting value of 93 ± 4 nM to a peak of 1121 ± 46 nM approximately 16 ms after the initiation of the electrical stimulus, returning to basal values after 112 to 128 ms of the depolarization. Ca2+ mobilization was inhibited by 90% in fibres pretreated with 10 μM nitrendipine, indicating that the electrically triggered [Ca2+]i transients were dependent on the activity of nitrendipine-sensitive voltage-operated Ca2+ channels. The amplitude of [Ca2+]i transients was significantly reduced under exposure to ethanol at doses of 100 mM and greater, ranging from 11 ± 3% at 100 mM to 22 ± 10 at 500 mM (P < 0.05), whereas the resting [Ca2+]i was unchanged (Table 1⇔). The reductions in [Ca2+]i transients were totally reversible after a 10-min washout period. Low ethanol concentrations (20 and 40 mM) did not exert a significant depressant effect on [Ca2+]i transients. The reduction caused in the [Ca2+]i transients correlated significantly with the dose of ethanol used (r = –0.48, P < 0.001).
|[Ca2+]i transients (nM)
|Values are in mM and are expressed as means ± SEM; n, number of fibres evaluated; *P < 0.05 and **P < 0.01 compared to basal conditions, respectively; †P < 0.05 compared to ethanol exposure.
|Control (zero ethanol) (n = 8)
|954 ± 52
|963 ± 62
|971 ± 78
|Ethanol 20 mM (n = 10)
|893 ± 134
|885 ± 144
|887 ± 125
|Ethanol 40 mM (n = 10)
|1164 ± 141
|1140 ± 134
|1205 ± 182
|Ethanol 100 mM (n = 10)
|1269 ± 231
|1045 ± 224*
|1347 ± 255†
|Ethanol 300 mM (n = 12)
|891 ± 140
|741 ± 95*
|831 ± 86†
|Ethanol 500 mM (n = 10)
|1011 ± 150
|631 ± 75**
|895 ± 120†
Effect of ethanol on the sarcolemmal Ca2+channel activity
The effect of ethanol on the rate and extent of cytosolic fura-2 quenching by extracellular Mn2+ during electrical stimulation was measured in isolated muscle fibres. Fluorescence (F360) data, collected every 2 s for 5 min, showed a very small decline of 3% in the Ca2+-independent fluorescence, due to photobleaching. When 0.2 mM MnCl2 was added to the buffer and electrical stimulation was not applied, F360 decreased by 10 ± 1% over the same period, a magnitude similar to that observed in fibres pretreated with nitrendipine (10 μM) and stimulated electrically. This showed that the basal quench was primarily independent of voltage-operated Ca2+ channels. By contrast, fibres exposed to a voltage-sensitive Ca2+ channel agonist Bay-K 8644 (1 μM) (LC Laboratories, Woburn, MA, USA) exhibited a threefold increase in the electrically induced Mn2+-quench of fura-2, compared with control fibres (P = 0.01).
Ethanol caused a dose-dependent reduction in the rate of Mn2+ quench (r = –0.57, P < 0.001), achieving an inhibition of 62 ± 11% at 500 mM ethanol (Fig. 1⇔). Moreover, the total amount of fura-2 quenched by Mn2+ during the 100 s under electrical stimulation was also reduced, achieving significance at low ethanol concentrations of 20 mM (21 ± 5%, P < 0.001). These data suggest that ethanol may interfere with sarcolemmal Ca2+ channel activity, diminishing Ca2+ entry into the cells.
Sarcoplasmic reticulum Ca2+loading state
The magnitude of [Ca2+]i transient also depends on the amount of Ca2+ released from the SR. Because the ethanol-induced decline in [Ca2+]i transient could be due to a reduction in the availability of SR Ca2+, we examined the SR Ca2+ content by measuring the caffeine-induced increase in [Ca2+]i. As the increase of [Ca2+]i evoked by caffeine could cause sarcolemmal depolarization and inward currents that might contribute to the [Ca2+]i signal (Renard et al., 1994), caffeine (15 mM) was applied in modified incubation buffer without Ca2+ and BSA. When caffeine was puffed in phase with the ongoing electrical pulse, more than 95% of fibres responded to caffeine, with similar Ca2+ mobilization at both the initial and a second exposure to caffeine 10 min later (753 ± 119 nM and 799 ± 156 nM, respectively). Ethanol at concentrations of up to 100 mM did not affect the caffeine-induced increase of [Ca2+]i (Table 2⇔). Only ethanol at 300 mM and above consistently decreased the magnitude of the caffeine-releasable Ca2+ pool, with a mean decrease of 35 ± 9% of control values for 300 mM ethanol (P = 0.004) (Table 2⇔).
|Ca release (nM)
|Values are in mM and are expressed as means ± SEM; n, number of fibres evaluated; *P < 0.05 and **P < 0.01 compared to basal conditions respectively.
|Control (zero ethanol) (n = 14)
|753 ± 119
|799 ± 196
|Ethanol 20 mM (n = 8)
|722 ± 127
|827 ± 246
|Ethanol 40 mM (n = 9)
|932 ± 260
|716 ± 266
|Ethanol 100 mM (n = 10)
|744 ± 260
|780 ± 185
|Ethanol 300 mM (n = 10)
|790 ± 136
|559 ± 127*
|Ethanol 500 mM (n = 8)
|762 ± 109
|465 ± 127**
The present study supports the hypothesis that ethanol interferes with EC coupling by decreasing the availability of Ca2+ needed to generate proper [Ca2+]i transients. Therefore, it is feasible that muscle weakness observed in very drunk patients as well as in the setting of acute and chronic alcoholic myopathies (Urbano-Márquez et al., 1989), may to some extent be mediated by the deleterious effect of ethanol on EC coupling. Acute ethanol may cause muscle weakness in the absence of histological evidence of myopathy (Fernández-Solà et al., 1996). In this sense, it is well known that the ergolytic effect of ethanol in athletes (Eichner, 1993) is more evident in middle distance running than in sprinting (McNaughton and Preece, 1986). Although acute exercise may lead to enzyme leakage as a result of damage to muscle fibres, Clarkson and Reichsman (1990) reported that ethanol did not cause any significantly greater increase in the plasma levels of muscle enzymes during exertion.
No recent study has evaluated the effect of ethanol on EC coupling in muscle fibres from mammalian origin. Preliminary studies with frog muscle preparations showed that alcohol reduced the contractile response and modified [Ca2+]i (Pape and Baylor, 1985; Oz and Frank, 1995). In stimulated mammalian whole-muscle specimens, ethanol exerted a marked decrease in the contractility at concentrations as low as 21.7 mM (Taylor et al., 1992; Pagala et al., 1995). However, the effect of ethanol on EC coupling at the level of mature skeletal muscle fibres of mammalian origin has not been studied. For this reason, we isolated mouse muscle fibres and loaded them with a fluorescent indicator, in order to assess the Ca2+-dependent steps of EC coupling. Ca2+ mobilization values obtained and the kinetics of the [Ca2+]i transients under control conditions were comparable to those reported in the literature when using similar models (Vergara et al., 1991; Imbert et al., 1995).
Similarly to other striated muscle specimens, we observed that ethanol acutely reversibly decreased [Ca2+]i transients in a dose-dependent manner (Thomas et al., 1989; Danziger et al., 1991; Nicolás et al., 1996), an effect that is mediated primarily by an inhibitory effect of ethanol on sarcolemmal voltage-operated Ca2+ channel activity (Takeda et al., 1984; Habuchi et al., 1995) and by reducing the amount of releasable Ca2+ available for EC coupling (Onishi et al., 1984; Thomas et al., 1996). Similar cellular effects have been described with a number of anaesthetic agents (Kress, 1995). By interfering with EC coupling in skeletal muscle cells, ethanol can exert deleterious consequences in muscle strength. We have already made the same observations when evaluating human cultured myotubes (Nicolás et al., 1998). Nevertheless, the inhibitory effects of ethanol on [Ca2+]i transients were somewhat lower than those documented in cardiomyocytes and cultured myotubes. While in the latter cells, 100 mM ethanol caused a decrease of about 25% in the magnitude of the [Ca2+]i transients (Nicolás et al., 1996, 1998), in the present study, mouse skeletal fibre [Ca2+]i transients were inhibited by 11%. This, we believe, is in accordance with the lower clinical consequences of ethanol misuse in skeletal muscle compared to heart muscle.
The maximum concentrations of ethanol used in this study were higher than those generally determined in alcoholics. Nevertheless, significant effects on Ca2+ channel activity and [Ca2+]i transients were observed with 20 to 100 mM (90–460 mg/dl) ethanol, which covers the range that can be encountered during binge drinking, and blood-ethanol concentrations as high as 300 mM have been encountered (Berlid and Hasselbalch, 1981; Johnson et al., 1982; O'Neill et al., 1984). Although the pharmacological effects at higher ethanol concentrations do not necessarily reflect all of the key changes in skeletal muscle physiology that occur at lower levels of ethanol, the data presented are consistent with the view that impairment of EC coupling may contribute to muscle weakness in alcoholism.
This work was supported with grants from Fondo de Investigaciones Sanitarias FIS 98/0330 and FIS 99/0115.
- © 2000 Medical Council on Alcoholism