text:
Biomaterials (v.26, #28)
Calendar (I).
Effect of cobalt and chromium ions on MMP-1, TIMP-1, and TNF-α gene expression in human U937 macrophages: A role for tyrosine kinases by Li Luo; Alain Petit; John Antoniou; David J. Zukor; Olga L. Huk; Roger C.W. Liu; Françoise M. Winnik; Fackson Mwale (5587-5593).
Previous reports have suggested that the imbalance of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) activity may contribute to prosthetic loosening. However, the mechanisms controlling these enzymes in the periprosthetic environment is unknown. We examined the effect of Co2+ and Cr3+ ions on the expression of genes encoding MMP-1, one of the principal proteinases capable of degrading native fibrillar collagens in the extracellular matrix (ECM), its inhibitor TIMP-1, and TNF-α, a cytokine that plays a central role in the induction of implant osteolysis. Human U937 macrophages were incubated in suspension or on phosphorylcholine (PC)-polymer coated surfaces for 24 h with Co2+ and Cr3+ ions. The level of mRNAs was determined by reverse transcription-polymerase chain reaction (RT–PCR). Results show that both Co2+ and Cr3+ ions induce the expression of MMP-1, TIMP-1, and TNF-α mRNA in a dose-dependent manner in cell suspensions. Tyrosine kinase inhibitors have different effects on these stimulatory effects. Indeed, genistein has only partial inhibitory effect on MMP-1 and TIMP-1, with even less effect on TNF-α expression. In contrast, herbimycin A completely blocks MMP-1 and TNF-α while partially inhibiting TIMP-1. However, Co2+ and Cr3+ ions had no effect on the expression of MMP-1 and TIMP-1 in macrophages cultured on the PC-polymer, suggesting that the attachment of U937 macrophages to the PC-polymer surfaces may modify their gene expression. In fact, MMP-1 and TIMP-1 seems to be constitutively up-regulated in this condition. However, the effect of Co2+ and Cr3+ ions on macrophages cultured on PC-polymer coated surfaces is similar to what was observed in suspension. Together, these findings indicate that activation of MMP-1, TIMP-1, and TNF-α by Co2+ and Cr3+ ions is regulated by tyrosine kinases.
Keywords: Cell adhesion; Hip replacement prosthesis; Macrophage; Matrix metalloproteinase; Metal ions; Phosphorylcholine;
Effect of vitamin E addition to poly(d,l)-lactic acid on surface properties and osteoblast behaviour by Filippo Renò; Valentina Aina; Silvia Gatti; Mario Cannas (5594-5599).
Vitamin E (Vit.E, α −tocoferol) is a natural agent with anti-oxidative and anti-inflammatory properties and it has been suggested that it could act as a stimulating factor for osteoblast proliferation and maturation. We produced poly(d,l)-lactic acid films enriched with Vit.E (1, 5 and 10% w/w) and investigated their surface properties using the FTIR analysis, sessile measure of wettability and serum protein adsorption, and evaluated attachment and spreading of MC-3T3 E1 murine osteoblast cells. FTIR analysis showed the presence of Vit.E on the polymer surface and Vit.E increased the polymer wettability in a concentration-dependent manner.The serum total protein adsorption increased significantly onto the 10% Vit.E P(d,l)-LA and the main protein adsorbed was albumin. The presence of albumin, considered as an anti-adhesive protein, on the surface of Vit.E enriched P(d,l)-LA films (especially 5 and 10% Vit.E) could explain, at least in part, the behaviour of MC-3T3 osteoblast cells seeded onto the polymers. Cell adhesion and spreading were strongly decreased by Vit.E (5 and 10%) in spite of the increased wettability. This reaction could be cell type-specific, independent by the surface wettability and linked to cell-specific characteristics (membrane phospholipid composition, integrins expression). Moreover a direct effect of Vit.E on cell adhesion and spreading cannot be completely excluded.
Keywords: P(d,l)-LA; Vitamin E; Osteoblast;
Bone formation and resorption of highly purified β-tricalcium phosphate in the rat femoral condyle by Naoki Kondo; Akira Ogose; Kunihiko Tokunaga; Tomoyuki Ito; Katsumitsu Arai; Naoko Kudo; Hikaru Inoue; Hiroyuki Irie; Naoto Endo (5600-5608).
The aim of this study was to examine the chronological histology associated with highly purified β-tricalcium phosphate (β-TCP) implanted in the rat femoral condyle. Specimens were harvested on days 4, 7, 14, 28 and 56 after implantation, and were analyzed by tartrate-resistant acid phosphatase (TRAP) staining, immunohistochemistry of the ED1 protein as a marker of the phagocyte system, and in situ hybridization with digoxigenin-labeled α1 chain of type I procollagen (COL1A1), osteopontin and osteocalcin. β-TCP was resorbed in a chronological manner. Although new bone was not observed on day 4, fibroblast-like cells around β-TCP were positive for COL1A1 and osteopontin mRNA. New bone formation presented after day 7. In the double-staining for OPN and ED1 on day 7, most cells around β-TCP were positive for either osteopontin mRNA or ED1 protein. However, there were some doubly positive multinucleated cells, suggesting that they belonged to the mononuclear phagocyte system. After day 28, the implanted region was replaced with bone marrow. Multinucleated TRAP-positive and ED1-positive cells which adhered to β-TCP at all stages seemed to be osteoclasts and they continuously resorbed β-TCP. β-TCP has a good biocompatibility since both bioresorption and bone formation started at an early stage after implantation.
Keywords: Bioresorption; Calcium phosphate; Macrophage; Osteoblast; Osteoclast;
Developing macroporous bicontinuous materials as scaffolds for tissue engineering by Monique Martina; Gayathri Subramanyam; James C. Weaver; Dietmar W. Hutmacher; Daniel E. Morse; Suresh Valiyaveettil (5609-5616).
Calcareous skeletal elements (ossicles) isolated from the seastar, Pisaster giganteus, were characterized and tested as potential biocompatible substrates for cellular attachment. These ossicles have a remarkably robust open-framework architecture with an interconnected network of ca. 10 μm diameter pores. Scanning electron and confocal microscopy was used to characterize the cell-substrate interaction. Cell culturing experiments revealed that the cells firmly attach to the ossicle surface, forming cell aggregates of several layers thick. The anchored cells extended to form 'bridges' between the openings in the bicontinuous framework and the degree of coverage increased as culture time progressed. Osteoblasts grown on the ossicles were found to be viable up to 32 days after initial seeding, as proven by assaying with AlamarBlue™ and FDA/PI staining indicating the ossicle's potential as an alternative highly effective tissue scaffold. Given the limitation in availability of this natural material, the results presented here should be seen as offering guidelines for future development of synthetic materials with physical and chemical properties strongly conducive to bone repair and restoration.
Keywords: Bone substitutes; Cell adhesion; Cell morphology; Bone graft; Osteoblast;
Preparation of γ -PGA/chitosan composite tissue engineering matrices by Chien-Yang Hsieh; Sung-Pei Tsai; Da-Ming Wang; Yaw-Nan Chang; Hsyue-Jen Hsieh (5617-5623).
γ -poly(glutamic acid) ( γ -PGA), a hydrophilic and biodegradable polymer, was chosen to modify chitosan matrices to produce a γ -PGA/chitosan composite biomaterial. Three types of both dense and porous composite matrices containing different amounts of γ -PGA were fabricated. Chitosan and γ -PGA matrices were also prepared as controls. Fluorescence staining indicated that chitosan and γ -PGA were evenly distributed in the composite matrices. SEM micrographs showed that an interconnected porous structure with a pore size of 30–100 μm was present in all porous matrices except the γ -PGA ones. By increasing the percentage of γ -PGA from 0% to 20%, the swelling ratio of the matrices was enhanced from 1.6 to 3.2. Similarly, the contact angle of the matrices decreased from 113° to 94°. These data suggested that the surface hydrophilicity, water absorption rate, and swelling ratio were improved by adding γ -PGA to the matrices. Additionally, the mechanical strength of the porous γ -PGA/chitosan matrices was about 25–50%, higher than that of the unmodified chitosan matrices. The composite matrices were also examined and found to be an appropriate environment for cell attachment and proliferation. The cell density on the 20% γ -PGA-modified matrices was almost triple that on the unmodified chitosan matrices on day 5. In summary, the γ -PGA/chitosan composite matrices, due to their better hydrophilic, cytocompatible, and mechanical properties, are very promising biomaterials for tissue engineering applications.
Keywords: Chitosan; γ -PGA; Tissue engineering; Composite matrices;
A synthetic nanofibrillar matrix promotes in vivo-like organization and morphogenesis for cells in culture by Melvin Schindler; Ijaz Ahmed; Jabeen Kamal; Alam Nur-E-Kamal; Timothy H. Grafe; H. Young Chung; Sally Meiners (5624-5631).
The purpose of this study was to design a synthetic nanofibrillar matrix that more accurately models the porosity and fibrillar geometry of cell attachment surfaces in tissues. The synthetic nanofibrillar matrices are composed of nanofibers prepared by electrospinning a polymer solution of polyamide onto glass coverslips. Scanning electron and atomic force microscopy showed that the nanofibers were organized into fibrillar networks reminiscent of the architecture of basement membrane, a structurally compact form of the extracellular matrix (ECM). NIH 3T3 fibroblasts and normal rat kidney (NRK) cells, when grown on nanofibers in the presence of serum, displayed the morphology and characteristics of their counterparts in vivo. Breast epithelial cells underwent morphogenesis to form multicellular spheroids containing lumens. Hence the synthetic nanofibrillar matrix described herein provides a physically and chemically stable three-dimensional surface for ex vivo growth of cells. Nanofiber-based synthetic matrices could have considerable value for applications in tissue engineering, cell-based therapies, and studies of cell/tissue function and pathology.
Keywords: Biomimetic material; Nanotopography; ECM; Cell culture;
Concentrated hydroxyapatite inks for direct-write assembly of 3-D periodic scaffolds by Sarah Michna; Willie Wu; Jennifer A. Lewis (5632-5639).
Hydroxyapatite (HA) scaffolds with a 3-D periodic architecture and multiscale porosity have been fabricated by direct-write assembly. Concentrated HA inks with tailored viscoelastic properties were developed to enable the construction of complex 3-D architectures comprised of self-supporting cylindrical rods in a layer-by-layer patterning sequence. By controlling their lattice constant and sintering conditions, 3-D periodic HA scaffolds were produced with a bimodal pore size distribution. Mercury intrusion porosimetry (MIP) was used to determine the characteristic pore size and volume associated with the interconnected pore channels between HA rods and the finer pores within the partially sintered HA rods.
Keywords: Hydroxyapatite; Bone tissue engineering; Scaffolds; Direct-write assembly;
Subcutaneous xenotransplantation of bovine pancreatic islets by Marina Figliuzzi; Roberta Cornolti; Tiziana Plati; Navneeta Rajan; Federica Adobati; Giuseppe Remuzzi; Andrea Remuzzi (5640-5647).
Transplantation of pancreatic islets in diabetes is currently limited by the need of immunosuppressive therapy. The present study was designed to test an immunoprotection planar device for subcutaneous xenotransplantation of pancreatic islets in the diabetic rat. We tested three different devices made of polyethersulfone hollow fibers. In all diabetic rats, implantation of islet-containing devices promptly normalized hyperglycemia. In vitro membrane permeability to glucose was correlated with implant function duration. These data confirm that bovine islets contained within devices and implanted subcutaneously remain functional for several days. Strategies to prolong islet function may allow achieving successful long-term islet implantation in this attractive site.
Keywords: Pancreatic islets; Immunoisolation device; Hollow fibers; Subcutaneous site; Xenotransplantation;
Bioactivation of an anorganic bone matrix by P-15 peptide for the promotion of early bone formation by Michael Thorwarth; Stefan Schultze-Mosgau; Falk Wehrhan; Peter Kessler; Safwan Srour; Jörg Wiltfang; Karl Andreas Schlegel (5648-5657).
This animal experiment compared the regenerative processes within defined bony defects of the porcine skull after delivery of routinely utilized bone graft materials: anorganic bone matrix (ABM) and an identical ABM carrying the cell binding peptide P-15. Particulated autogenous bone was used as a control group. The chosen porcine model guaranteed the transferability of the obtained results to clinical practice. A total observation period of 6 months was defined. The bone samples were examined microradiographically and histologically at 8 specific times. Sufficient osseointegration and osseoconduction could be demonstrated for both anorganic bone minerals. However, in the selected model significantly higher mineralization rates ( p = 0.0286 ) were found in the microradiographic image at 12 weeks after application of the bioactive form. The histological examination confirmed this accelerating effect on bone formation starting at day 3. At the end of the study after 6 months, the mineralization values had equalized in both study groups.For the first time, the material was demonstrated to be suitable as a bone substitute material for the treatment of larger bony defects in a large animal model. The P-15 sequence accelerated the process of bone formation on the surface of the anorganic bone matrix as early as 3 days but was not traced over the whole term of the study.
Keywords: Bone regeneration; Anorganic bone matrix; Hydroxyapatite ceramics; P-15 peptide; Bone graft; Collagen;
Effect of passage number and matrix characteristics on differentiation of endothelial cells cultured for tissue engineering by Krishna Prasad Chennazhy; Lissy K. Krishnan (5658-5667).
Cells can sense the physical and chemical properties of artificial materials used as scaffolds for tissue engineering and regulate their behavior. Therefore, biomimetic and biospecific molecules are coated on materials to regulate function of cells on the tissue-engineered product. These bioactive molecules can be attached in a defined spectrum, concentration and spatial distribution in order to control adhesion, growth, viability, differentiation, and function of the cells. When autologous cells are used for tissue engineering, initially limited cells obtained may often need an amplification of cell number by passage in tissue culture before they are seeded on a biomaterial or scaffold. We have conducted this study to understand how the characteristics of bioactive molecule coating might affect proliferation, apoptosis and differentiation when endothelial cell (EC) is serially passaged. Proliferation was assessed by proliferating cell nuclear antigen (PCNA) staining along with counting of cells harvested from confluent monolayer. Apoptosis was assessed by Annexin V staining and differentiation by semi quantitative reverse transcriptase polymerase chain reaction (RT-PCR) for von Willebrand factor (vWF) expression and quantification of its release using enzyme linked immunosorbant assay (ELISA), and thrombogenicity by comparing platelet adhesion to EC monolayer Dacron grafts (DG) with specific protein coating. The results indicate that ECs easily lose its proliferation potential when they are cultured repeatedly on gelatin, turn apoptotic and over express the prothrombotic protein- vWF. Whereas, when it is grown on a matrix composed of fibrin, fibronectin, gelatin and vascular EC growth factor (VEGF), the cells retained their ability to proliferate, remained viable and were relatively less thrombogenic, even when passage number progressed. It is concluded that if ECs are grown on the composite matrix that mimics natural vessel scaffold, the cell number can be amplified without affecting its normal physiological function and may be used to generate effective tissue-engineered cardiovascular constructs.
Keywords: Endothelial Cell; Vascular graft; Extracellular matrix; FIBRIN; Gelatin; Phenotypic alteration;
Down regulation of degenerative cartilage molecules in chondrocytes grown on a hyaluronan-based scaffold by Brunella Grigolo; Luciana De Franceschi; Livia Roseti; Luca Cattini; Andrea Facchini (5668-5676).
Hyaluronic-acid-based biomaterials used for cartilage repair allow the expression of specific extracellular matrix molecules by human chondrocytes grown onto them. We investigated whether these biomaterials could also create an environment in which the cells downregulate the expression of some catabolic factors.Chondrocytes were isolated from human articular cartilage obtained from the knees of patients with a history of trauma. First, the cells were expanded in monolayers and then they were seeded on a hyaluronic-acid derivative scaffold. Constructs and surnatants were collected and analysed at 1, 3, 7, 14 and 21 days after seeding. Immunohistochemical analysis for CD44 and caspase was carried out on paraffin-embedded sections. The Tunel method was used to identify chondrocyte apoptosis status. Secretion of MMP-1 and MMP-13 in the surnatants of the cells grown onto the biomaterial was measured by enzyme-linked immunosorbent assay. Nitric oxide (NO) production was evaluated by estimating the stable NO metabolite nitrite by the Griess method. A real-time RT-PCR analysis was performed on the constructs to evaluate the expression of type I and II collagens, aggrecan, Sox-9, MMP-1 and MMP-13 mRNAs at the different experimental times evaluated.Decreased levels of metalloproteinases and nitric oxide were observed in the surnatants of chondrocytes grown onto the hyaluronan-based scaffold. This was also confirmed by real-time PCR analysis which showed that the cells expressed the specific differentiated phenotype downregulating the expression of some catabolic molecules. Cells apoptosis decreased during the culture period, which further supported the biochemical data.The ability of the hyaluronan scaffold to reduce the expression and production of molecules involved in cartilage degenerative diseases indicates its use to treat early lesions of osteoarthritic patients.
Keywords: Chondrocytes; Hyaluronic acid; Arthritis; Apoptosis; Metalloproteinases;
Cellular and molecular events during chondrogenesis of human mesenchymal stromal cells grown in a three-dimensional hyaluronan based scaffold by Gina Lisignoli; Sandra Cristino; Anna Piacentini; Stefania Toneguzzi; Francesco Grassi; Carola Cavallo; Nicoletta Zini; Liliana Solimando; Nadir Mario Maraldi; Andrea Facchini (5677-5686).
Mesenchymal stromal cells (MSCs) seem to be a good alternative to chondrocytes for cartilage regeneration. To obtain new information on the sequence of cellular and molecular events during in vitro chondrogenic differentiation we analysed MSCs on a widely used hyaluronic acid biomaterial (Hyaff®-11). Cellular differentiation was induced using two different concentrations of TGF β 1 (10 and 20 ng/ml) and the process was analysed at different time points (24 h, and 7, 14, 21 and 28 days) using techniques of light and electron microscopy, real-time PCR and immunohistochemistry. We found that without TGF β MSCs did not survive while in the presence of TGF β the cells significantly proliferated from day 7 until day 28. Light and electron microscopy showed that TGF β at 20 ng/ml better induced the formation of cartilage-like tissue. Real-time PCR showed an increased expression of collagen type II, IX and aggrecan associated to a down-regulation of collagen type I. Immunohistochemical analysis confirmed that collagen type I was down-modulated while collagen type II increased from day 14 to day 28.These data clearly showed that higher concentrations of TGF β (20 ng/ml) induce chondrogenesis of MSCs on Hyaff®-11 scaffold better than 10 ng/ml of TGF β . This process is characterized by a sequence of cellular and molecular events that deal with the in vitro formation of a cartilage-like tissue.
Keywords: Mesenchymal stromal cells; Chondrogenesis; Biomaterial; Tissue engineering;
Effect of bone mineral density and amorphous diamond coatings on insertion torque of bone screws by Arto Koistinen; Seppo S. Santavirta; Heikki Kröger; Reijo Lappalainen (5687-5694).
In this study, the potential of high-quality amorphous diamond (AD) coatings in reducing the torque and failures of bone screws was studied. Torque values were recorded for 32 stainless steel screws, 2.7 or 3.5 mm in diameter and 60 mm in length. Half of the screw sets were coated with the AD coating before installing in predrilled holes of human cadaveric femoral bone samples. The bone samples were selected from two groups of four persons with mean ages of 34 years (range 25–41 years) and 75 years (range 73–77 years), respectively. The bone mineral density (BMD) values of the samples were determined exactly at the screw insertion site by peripheral quantitative computed tomography (pQCT). In the mechanical tests, insertion and removal torques were measured. BMD had a significant effect on insertion torque; the maximum torque (adjusted with respect to the screw diameter) was significantly higher for the young bone than for the old bone ( p < 0.05 ). By using a polished AD coating, insertion torque was decreased even up to 50% in comparison with the screws without coating. The results suggest that AD coating provides a stable, smooth surface and reduces the risk of screw failures.
Keywords: Biocompatibility; Bone repair; Diamond; Mechanical test; Surface treatment;
Fe-based nanoparticulate metallic alloys as contrast agents for magnetic resonance imaging by Oscar Bomatí-Miguel; María P. Morales; Pedro Tartaj; Jesús Ruiz-Cabello; Pierre Bonville; Martín Santos; Xinqing Zhao; Sabino Veintemillas-Verdaguer (5695-5703).
Pharmaceutical grade magnetic colloidal dispersions have been prepared from iron alloys synthesized by laser pyrolysis. The colloids were obtained by simultaneous dispersion and coating of the particles with dextran in a strong alkaline solution. Both powders and dispersions have been analyzed in terms of microstructural characteristics, chemical composition and magnetic properties. The powders consist of uniform spherical nanoparticles (12 nm of diameter) showing a metallic core encapsulated into an iron-oxide shell. On the other hand, the colloidal dispersions consist of magnetic particles-aggregates with hydrodynamic sizes of ∼75 nm. Magnetic resonance images of rats were taken after the intravenously administration of the Fe colloidal dispersions, and compared with those obtained using a commercial iron oxide magnetic resonance imaging contrast agent. The results showed a contrast improvement of 60% in the liver with respect to the commercial sample, which suggests that this product could be a suitable contrast agent for NMR imaging of liver and spleen.
Keywords: Laser; Nanoparticles; Magnetism; Magnetic resonance imaging (MRI); Liver;
Steady-state diffusion of water through soft-contact-lens materials by Francesco Fornasiero; Florian Krull; John M. Prausnitz; Clayton J. Radke (5704-5716).
Water transport through soft contact lenses (SCL) is important for acceptable performance on the human eye. Chemical-potential gradient-driven diffusion rates of water through SCL materials are measured with an evaporation-cell technique. Water is evaporated from the bottom surface of a lens membrane by impinging air at controlled flow rate and humidity. The resulting weight loss of a water reservoir covering the top surface of the contact-lens material is recorded as a function of time.New results are reported for a conventional hydrogel material (SofLens™ One Day, hilafilcon A, water content at saturation w 10 = 70 weight %) and a silicone hydrogel material (PureVision™, balafilcon A, w 10 = 36 % ), with and without surface oxygen plasma treatment. Also, previously reported data for a conventional 2-hydroxyethyl methacrylate (HEMA)-SCL ( w 10 = 38 % ) hydrogel are reexamined and compared with those for SofLens™ One Day and PureVision™ hydrogels. Measured steady-state water fluxes are largest for SofLens™ One Day, followed by PureVision™ and HEMA. In some cases, the measured steady-state water fluxes increase with rising relative air humidity. This increase, due to an apparent mass-transfer resistance at the surface (trapping skinning), is associated with formation of a glassy skin at the air/membrane interface when the relative humidity is below 55–75%.Steady-state water fluxes are interpreted through an extended Maxwell–Stefan diffusion model for a mixture of species starkly different in size. Thermodynamic nonideality is considered through Flory–Rehner polymer-solution theory. Shrinking/swelling is self-consistently modeled by conservation of the total polymer mass. Fitted Maxwell–Stefan diffusivities increase significantly with water concentration in the contact lens.
Keywords: Evaporation-cell method; Water diffusion-coefficient; Soft contact lens; Extended Maxwell–Stefan model; Trapping skinning; Glass transition;
The effect of the antimicrobial peptide, Dhvar-5, on gentamicin release from a polymethyl methacrylate bone cement by C. Faber; R.J.W. Hoogendoorn; D.M. Lyaruu; H.P. Stallmann; J. van Marle; A. van Nieuw Amerongen; T.H. Smit; P.I.J.M. Wuisman (5717-5726).
The objective of this study was to investigate the release mechanism and kinetics of the antimicrobial peptide, Dhvar-5, both alone and in combination with gentamicin, from a standard commercial polymethyl methacrylate (PMMA) bone cement. Different amounts of Dhvar-5 were mixed with the bone cement powders of Osteopal and the gentamicin-containing Osteopal G bone cement and their release kinetics from the polymerized cement were investigated. Additionally, the internal structure of the bone cements were analysed by scanning electron microscopy (SEM) of the fracture surfaces. Secondly, porosity was investigated with the mercury intrusion method and related to the observed release profiles. In order to obtain an insight into the mechanical characteristics of the bone cement mixtures, the compressive strength of Osteopal and Osteopal G with Dhvar-5 was also investigated. The total Dhvar-5 release reached 96% in the 100 mg Dhvar-5/g Osteopal cement, whereas total gentamicin release from Osteopal G reached only 18%. Total gentamicin release increased significantly to 67% with the addition of 50 mg Dhvar-5/g, but the Dhvar-5 release was not influenced. SEM showed an increase of dissolved gentamicin crystals with the addition of Dhvar-5. The mercury intrusion results suggested an increase of small pores (<0.1 μm) with the addition of Dhvar-5. Compressive strength of Osteopal was reduced by the addition of Dhvar-5 and gentamicin, but still remained above the limit prescribed by the ISO standard for clinical bone cements. We therefore conclude that the antimicrobial peptide, Dhvar-5, was released in high amounts from PMMA bone cement. When used together with gentamicin sulphate, Dhvar-5 made the gentamicin crystals accessible for the release medium presumably through increased micro-porosity (<0.1 μm) resulting in a fourfold increase of gentamicin release.
Keywords: Polymethylmethacrylate; Drug release; Porosity; Gentamicin; Dhvar-5; Antimicrobial peptide;
Surface modification of biodegradable polyesters with fatty acid conjugates for improved drug targeting by Tarek M. Fahmy; Robert M. Samstein; Casey C. Harness; W. Mark Saltzman (5727-5736).
We describe a general method for incorporating target ligands into the surface of biocompatible polyester poly(lactic-co-glycolic acid) (PLGA) 50/50 materials using fatty acids. Avidin-fatty acid conjugates were prepared and efficiently incorporated into PLGA. Avidin was chosen as an adaptor protein to facilitate the attachment of a variety of biotinylated ligands. We show that fatty acid preferentially associates with the hydrophobic PLGA matrix, rather than the external aqueous environment, facilitating a prolonged presentation of avidin over several weeks. We successfully applied this approach in both microspheres encapsulating a model protein, bovine serum albumin, and PLGA scaffolds fabricated by a salt-leaching method. Because of its ease, generality and flexibility, this strategy promises widespread utility in modifying the surface of PLGA-based materials for applications in drug delivery and tissue engineering.
Keywords: Biodegradation; Biocompatibility; Surface modification; Lipid; Drug delivery; Microsphere; Scaffold;
Chitosan beads as molecularly imprinted polymer matrix for selective separation of proteins by Tian-Ying Guo; Yong-Qing Xia; Jin Wang; Mou-Dao Song; Bang-Hua Zhang (5737-5745).
Two kinds of molecularly imprinted polymers were prepared using hemoglobin as the imprinting molecule, acrylamide as the functional monomer, chitosan beads and maleic anhydride-modified chitosan beads as matrixes, respectively. Static adsorbing experimental results showed that an equal class of adsorption was formed in the imprinted polymers and the adsorption equilibrium constant and the maximum adsorption capacity were evaluated. Chromatographic characteristics showed that the column bedded with the hemoglobin imprinted beads could separate hemoglobin and bovine serum albumin effectively from their mixture, which indicates that the imprinted beads have very higher selectivity for hemoglobin than the non-imprinted with the same chemical composition.
Keywords: Chitosan beads; Polyacrylamide; Protein separation; Molecular imprinting;
Collection of neural inducing factors from PA6 cells using heparin solution and their immobilization on plastic culture dishes for the induction of neurons from embryonic stem cells by Hironori Yamazoe; Yoshinobu Murakami; Kenji Mizuseki; Yoshiki Sasai; Hiroo Iwata (5746-5754).
Embryonic stem (ES) cells have the ability to replicate themselves and differentiate into various mature cells. Recently, dopaminergic neurons were efficiently induced from ES cells using mouse stromal cells (PA6 cells) as a feeder cell layer. This simple procedure seems to be very efficient to obtain dopamine-releasing cells for future clinical cell transplantation treatment of Parkinson's disease. In this study, we prepared stock solutions containing neural inducing factors (NIFs) by washing PA6 cells with phosphate-buffered saline containing heparin. ES cells grew successfully in culture media supplemented with 33 v/v% NIFs stock solution, and the rate of neural differentiation of ES cell progeny increased with increasing heparin concentration in the culture media. In addition, NIFs-immobilized surfaces were prepared by exposing polyethyleneimine-modified surfaces to NIFs stock solutions. The NIFs-immobilized culture dish effectively supported cell growth as the culture medium supplemented with NIFs stock did, but its induction effect to dopaminergic neurons from ES cells was much smaller than free NIFs. NIFs stock solutions have two different activities. One can stimulate cell growth and the other induces differentiation of ES cells to the neural fate when heparin existed. The former factors were effectively immobilized on the culture dish, but those that induce differentiation may not be. Further optimization is required.
Keywords: Embryonic stem cell; Neural inducing factors; PA6 cell; Heparin; Neural cell; Surface modification;


text:
Fast Fiesta Soup RecipeRead Reviews Be the first to add a review
4.5 3 4
"This spicy soup was served at a very elegant lunch, and the hostess was deluged with requests for the recipe," reports Patricia White of Monrovia, California. The colorful combination is a snap to throw together...just open the cans and heat.
- 2 cans (10 ounces each) diced tomatoes and green chilies
- 1 can (15-1/4 ounces) whole kernel corn, drained
- 1 can (15 ounces) black beans, rinsed and drained
- Shredded cheddar cheese and sour cream, optional
- In a large saucepan, combine the tomatoes, corn and beans; heat through. Garnish serving with cheese and sour cream if desired. Yield: 4 servings.
Originally published as Fast Fiesta Soup in Quick Cooking March/April 2000, p11
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Reviewed Feb. 27, 2011
"This soup is so easy to make--simply a matter of opening cans. It was a bit hot for my taste, so next time I will use one can tomatoes and chilies and 1 can plain tomatoes. The sour cream garnish adds much to the soup"
Reviewed Aug. 30, 2010
"This is really easy to make & very tasty. All the flavors work really well. I will make this again."
Reviewed Mar. 6, 2010
"This could not be easier! EVERYONE likes this soup and I am always asked for the recipe. A great change of pace and delicious."

subset: commoncrawl


text: Designed specifically for graphite dust control, the Wynn "G" series flanged cartridges from Wynn Environmental are directly interchangeable with filters commonly used in vibrating-type dust collectors. These surface-loading, Merv 15 nanofiber filters have an open-pleat design for dust release during the cleaning cycle. The high-filtration efficiency reduces dust bypass, and there is no need for warp-around pre filters, the company says.


text:
The Bridge
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- Brief Synopsis:
- Number one New York Times bestselling author Karen Kingsbury delivers an instant classic with this heartwarming Christmas story about a hundred-year flood, lost love, and the beauty of enduring friendships.
- Long Synopsis:
- Number one New York Times bestselling author Karen Kingsbury delivers an instant classic with this heartwarming Christmas story about a hundred-year flood, lost love, and the beauty of enduring friendships. Molly Allen lives alone in Portland, but she left her heart back in Tennessee with a man she walked away from five years ago. They had a rare sort of love she hasn't found since. Ryan Kelly lives in Nashville after a broken engagement and several years on the road touring with a country music duo. He can still hear Molly's voice encouraging him to follow his dreams; Molly, whose memory stays with him. At least he can visit The Bridge--the oldest bookstore in historic downtown Franklin--and remember the hours he and Molly once spent there. For thirty years, Charlie and Donna Barton have run The Bridge, providing the people of middle Tennessee with coffee, conversation, and shelves of good books--even through dismal book sales and the rise of digital books. Then in May, the hundred-year flood swept through Franklin and destroyed nearly every book in the store. Now the bank is pulling the lease on The Bridge. Despondent and without answers, Charlie considers the unthinkable. Then tragedy strikes, and suddenly, everything changes. In the face of desperate brokenness and lost opportunities, could the miracle of a second chance actually unfold? The Bridge is a love story set against the struggle of the American bookstore, a love story you will never forget.
- Book Quality:
- Publisher Quality
- 9781451647020
- Publisher:
- Howard Books
- Date of Addition:
- 08/04/14
- Copyright Date:
- 2012
- Copyrighted By:
- Karen Kingsbury
- Adult content:
- No
- Language:
- English
- Categories:
- Nonfiction, Religion and Spirituality, Romance, Literature and Fiction
- Submitted By:
- Bookshare Staff
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text:
GROW-Services provides GROW charts integrated in your maternity information system or through the standalone Web-app
Customised charts (GROW) delineate the Gestation Related Optimal Weight for each baby, by
Literature summarising the evidence for customised charts is available on www.gestation.net. Their application within a comprehensive training programme with standardised protocols have been shown to significantly reduce stillbirth rates in England
The charts are recommended by the UK Royal College of Obstetricians and Gynaecologists RCOG and The Royal Dutch Midwifery Association KNOV.
The GROW Services enable Maternity Information Systems to incorporate GROW charts in the application. If the MIS integration is not yet available GROW-Services also provides the charts through an standalone Web-app.
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The standalone Web-app is for healthcare providers that work with an MIS that does not yet support direct integration and features:
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Peter Sprigg: Run Lisa Run
Does anyone find it entirely predictable that the Family Research Council's Peter Sprigg, who thinks that gay behavior should be outlawed and carry criminal penalties, supports Lisa Miller's kidnapping of her daughter and disappearance so as to avoid adhering to court ordered custody arrangements?
Imagine that you are a mother (perhaps you are). You have only one child -- your own flesh and blood, conceived with your egg, borne in your body, pushed out into the world by your own exertions.
She is seven and a half years old, and for the last six years you have been her sole caretaker. Single parenthood is tough, but your parents help, and it seems your daughter is doing well. She is happy and well-adjusted. You have worked hard to support her and transmit your values to her. She goes to church with you every week.
Now, someone wants to take her away from you. Her father? No -- another woman wants to be her mother. This woman lives in a different state hundreds of miles away. Your daughter once knew the woman, but so long ago that she has no memory of her. The woman has no biological relationship to your child. She has no adoptive relationship to your child. But she wants to take your daughter away from you and be her mother now.
No court has ever found you to be an unfit mother. And yet -- unbelievably -- the courts of two states have ordered you to transfer custody of your child to this other woman.
What would you do? Do you simply give your child away?
This is not the plot for a Hollywood thriller. This nightmare scenario is the real-life situation that Lisa Miller found herself in recently.
Apparently, Lisa couldn't give her daughter away. She chose to run instead.
Of course, there is more to the story. But if reading the description above leaves you with a sick feeling in the pit of your stomach, it should. This is the brave new world of family law, thanks to the gains made by the homosexual movement.
Lisa was told to transfer custody of her daughter on New Year's Day. She never showed up.
Would you?
In Sprigg's world, it's gays who are the criminals while ex-gay Christian women kidnap their daughter and violate court orders are heroes.
And on a related note, in my post yesterday on Sprigg's "gay behavior should be illegal" comment, I wondered if FRC would make him issue an apology. Well, apparently they won't be since they are featuring his "Hardball" appearance on their website and touting it in their most recent "Washington Update":
Judging by our busy press room, FRC continues to be the go-to organization on this issue in the media. Apart from a series of print interviews, Peter Sprigg and I took the lead on a few national talk shows yesterday, debating the fallout of homosexuals in the military with experts from the other side. You can watch all three appearances--on CNN, MSNBC's "Hardball," and "Larry King Live"--by visiting our newsroom.
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People:Peter Sprigg , Lisa Miller
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Monday, January 14, 2013
Thomas Edward Kascak, 64, of Summerville, SC, loving husband of Susan Elizabeth Kascak entered into eternal rest while vacationing in Colorado; Tuesday, December 25, 2012.
A Mass of Resurrection will be held Saturday, January 12, 2013, at St. John the Beloved Catholic Church, 28 Sumter Avenue, Summerville at 11:00 a.m. to celebrate his life. In lieu of flowers memorials can be made to Meals On Wheels of Summerville, PO Box 592 Summerville, SC 29484 or the Society for Prevention of Cruelty to Animals, 100 Four Paws Lane, Summerville, SC 29483.
Thomas was born December 2, 1948, in Cleveland, OH, the son of Thomas M. Kascak and Ann Marie Sabol Kascak.
He was a retired Controller from JM Steel Corporation. He was a member of St. John the Beloved Catholic Church.
Surviving in addition to his wife of 42 years, Susan Kascak, are his parents Thomas and Anne Marie Kascak of Beachwood, OH; three sons: Thomas Joseph Kascak (Melissa) of Charleston, SC, David Michael Kascak (Kimberly) of James Island, Matthew Douglas Kascak (fiancée Magdalena Dohnalova) of Denver, CO; one granddaughter, Sadie Lane Kascak of James Island; one brother Kevin Kascak (Nancy) of Twinsburg, OH, 11 nieces and nephews, 13 great nieces and nephews and many dear friends. He is predeceased by his sister, Karen Kascak Diemer.
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Homeland Security chief Jeh Johnson doubts border impact of Texas Guard
WASHINGTON – Homeland Security Secretary Jeh Johnson questioned the need for Texas to send National Guard troops to the border, asserting Tuesday that the crisis abated by the time state leaders reacted last summer.
"The high point of the … unaccompanied kids crossing into South Texas last year was June 10th," Johnson told a small group of reporters at the White House, suggesting that Texas officials, particularly then-Gov. Rick Perry, claim too much credit for tamping down the influx of migrants.
"I don't know what role the Guard played" three months after the peak, he said.
Perry, who left office last month, ordered 1,000 guardsmen to the border under Operation Strong Safety in late July. The troops began arriving in late August.
By then, Johnson noted, the number of people crossing the border illegally – many of them unaccompanied minors from Central America, "had already dropped dramatically…. By mid-August it was down to very low levels, and it pretty much stayed that way."
Perry, jockeying for the 2016 GOP presidential nomination, regularly reminds voters about his efforts to beef up border security, to bolster his own image and blast the Obama administration.
Johnson defended the federal response last summer.
"We expanded our detention space. We surged resources, people and equipment down to the southern border. We cranked up prosecutions of coyotes [smugglers]" along with a public messaging campaign, and flights taking would-be immigrants back to Central America. "We did about 10 different things."
In any case, he added, "A lot of illegal migration is seasonal. It creeps up in the spring, it spikes in early summer, then it starts to drop off when it gets really hot," making it hard to say how much impact the Texas Guard deployment had.
On other topics, Johnson:
- Complained that Congress hasn't approved funds for the extra border security. He covered costs by diverting hundreds of million of dollars from a disaster relief fund. Fortunately, he said, the weather cooperated last year. But he added, it's no accident that apprehensions at the border hit a three-year low in January. Extra security was added, "which we need Congress to pay for. If you want a more secure border, that's not free."
- Warned of dire consequences if Congress allows DHS funding to expire at midnight Saturday. Some Republicans want to cut funding needed to implement executive actions issued in November, shielding up to 5 million people from deportation. "If Congress wants to have a debate about immigration, we should have the debate about immigration," he said. But "it's bad politics to leverage the immigration debate to the budget for homeland security for this nation." At least three-fourths of the DHS workforce would have to work without being paid until the stalemate ends, because they're engaged in law enforcement, border security and other critical functions. Some 30,000 workers would be furloughed. That, Johnson said, could hamper efforts to combat cybersecurity, and to monitor terrorists and bad weather. Most of the workforce at the Federal Emergency Management Agency would be furloughed. Grants to local and state law enforcement would be delayed. "It's like driving across country on 5 gallons of gasoline at a time and you don't know where the next gas station will be," he said.
- Warned that New York City could face extra danger. "A shutdown of the Department of Homeland Security has real consequences to homeland security and public safety. Period, end of sentence," he said.
- Argued that public perceptions don't match reality on border security. He cited a Pew survey from mid-2013 that found 55 percent of Americans believe illegal immigration is worse. In fact, the number of apprehensions at the border has dropped from about 1.6 million in 2000 to 479,000 in fiscal 2014 – and that was up from the previous year. "The numbers are a fraction of what they used to be," he said.


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Harken USA shipping and receiving will be closed
for physical inventory from 11:00 AM CST, Wednesday, September 28th through
Friday, September 30th. Other departments will be available as usual. We will
resume shipping and receiving orders on Monday, October 3rd.
Repair kits are available for all valves, cylinders, and pumps. They include everything in the seal kit with the addition of select machined components that may require occasional replacement.
DO NOT use Harken equipment for human suspension unless product is specifically certified and labeled for such use.
Address InformationTel: 262.691.3320 | Fax: 262.701.5780 Email: email@example.com Technical Service: firstname.lastname@example.com our other locations


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Billie Jeanne Hartness Davis, 83, of Canyon died Wednesday, March 5, 2003, in Amarillo.
Memorial services will be at 4 p.m. today in First Presbyterian Church with the Rev. Don McDonald, pastor, officiating. Burial will be private. Arrangements are by Brooks Funeral Directors.
Mrs. Davis was born Aug. 28, 1919, in Perrin. She was the sixth of seven children born to Ruda Priscilla Lee and John Hartness. Billie took piano lessons as a young girl, and while still in grade school, traveled to various cities in north central Texas giving recitals and playing for church groups.
After graduating from Adamson High School in Dallas, Billie played for dance classes and worked for an advertising agency in Dallas where she met Walter Ragland Davis. The couple married in Rosemont Christian Church in Dallas on Billie's birthday in 1940. Their first child, Walter R. Davis II was born June 13, 1942, while Walter was working at North American Aviation during World War II. Their second child, Patricia Ann Davis Hamilton was born Feb. 28, 1947.
In the ensuing years Billie played piano and organ for her church, raised a family, and worked side-by-side with Walter in several restaurants they owned and managed. After her children left home, Billie worked for Texas Employment Commission in Dallas. She moved to Canyon in 2001, where she was a member of First Presbyterian Church.
She was preceded in death by her husband in 1991; two sisters; and three brothers.
Survivors include a sister, Ruda Johnson of Canyon; a son, Walt Davis and wife, Isabel, of Canyon; a daughter, Patti Hamilton and husband, Cla, of Justin; four grandchildren, John Davis and wife, Tracey, of Dallas, Lee Davis and wife, Melissa, of McKinney, Kathy Hamilton Lee and husband, Ron, of Hurst and Benjamin Hamilton of Denton; and three great-grandchildren, James Lee and Madeline Lee, both of Hurst, and Quin Davis of McKinney.
The family suggests memorials be to Billie Jeanne Hartness Davis Music Scholarship at West Texas A&M University.
Amarillo Globe-News, March 7, 2003
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Spain Food History
Spain has a very long history of being invaded which had influenced Spanish food. From Greek and Roman culture came olives, olive oil and wine. From the Celts came the fish and meat pies and the Moors contributed ingredients such as citrus fruits, spices, almonds, honey, cumin and saffron. During Roman times, lentils were already eaten by soldiers in the army. Fava beans were also commonly eaten and the Romans thought they were sacred.
Spanish Food - Spanish Cuisine
Food by Country » European Food » Spanish Food - Spanish Cuisine, Food in Spain
Spanish Cuisine
History of Spain
Spain Food History
Spain Food
Traditional Food in Spain
There are many different types of food in Spain, but only a few are common throughout the country. While some of these dishes can be traced back to a specific area, they are still considered common to the country. Many favorites have a bean base, with ingredients such as lentils, green beans and chickpeas. Also common are breads and soups.
A traditional breakfast in Spain is made up of churros that have been covered with sugar and dipped in hot chocolate. Lunch, also known as comida is the main meal of the day and often is larger than dinner. Lunch is made up of many courses with the first being soup or salad and the second being a meat or fish dish. Dessert follows and is often a piece of cake or fruit. Dinner in Spain often consists of a sandwich or salad.
Spanish Foods
Popular Dishes in Spain
- Tumbet - This traditional dish is made of vegetables. It has layers of potato slices, red bell peppers that have been fried in olive oil and aubergines.
- Escalivada - This is a salad made of many different kinds of vegetables that have been grilled. Ingredients include red peppers, red tomatoes, sweet onions and egg plants.
- Pescado frito - This is an old dish of fish that has been deep fried in vegetable oil to keep it crisp and light.
- Gazpacho - This is a raw vegetable soup. It is tomato based and comes from the southern parts of Andalusia.
- Fabada Asturiana - This stew has white beans, morcilla, pork shoulder, saffron and chorizo.
- Paella - This rice dish is Valencian. It is made of green vegetables, meat, white rice, beans, land snails and different seasonings.
- Torta de Aceite - This is a crispy and flaky biscuit. It is made up of extra virgin olive oil, wheat flour, sesame seeds, almonds anise flavor and anise seeds.
- Spanish omelette - This is a common Spanish meal consisting of an omelet that is made of eggs and fried potatoes.
- Marmitako - This is a traditional stew made of fish, onions, tomatoes and potatoes.
- Cozido - This is a traditional Spanish dish. It is a stew that is made of vegetables and meat.
Spanish Drinks
Popular Spanish Drinks
- Zurracapote - This is a favorite alcoholic mixed drink. It is made of red wine and fruits such as lemons and peaches.
- Sidra - This is a popular drink that is made out of fresh apples.
- Brandy de Jerez - This is a very famous brandy made in Spain.
- Wine - This is the Spanish national drink. Some of the more famous wines include Graciano, Mencia, Godello, Loureira and Carinena.
Information on Spain
Discover more Spanish Foods and Drinks Resources
- Spain Background Information - provided by U.S. Department of State
- Traditional Food in Spain - popular dishes and favorite foods.
- Vacation in Spain - travel guides.
- Typical Drinks in Spain: brandy, wine.
- Spanish Dessert - a typical Spanish dessert which is served at various restaurants.


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Eddie Clark Jr. brings comedy joy to Jr.'s Last Laugh
When Eddie Clark Jr. attended college in 1979, he agreed to emcee a talent show. After the judges saw the contestants and prepared to vote, he learned prize money was at stake.
"I said, 'Excuse me, judges, I have one last performer who's not on the bill,'" Clark recalled. "I got them to sit back down, introduced myself and did (comedy) and won third place. I got $25 out of it. When you're in college, $25 is the world. I was Donald Trump!"
The New York reference fits. Clark grew up in Queens at a time when comedy clubs exploded with fresh talent. Robin Williams even encouraged him after Clark failed to impress a comedy-club owner.
"I was lucky," said Clark, who performs at Jr.'s Last Laugh tonight through Saturday. "I came along at a great time; I got a chance to open for a lot of great stand-ups -- Sam Kinison and Jerry Seinfeld and Rita Rudner. I got to open for Rose O'Donnell, Mario Joyner, Jimmy Tingle.
"Bill Hicks and Sam Kinison, those two guys were the nicest guys in the world to me. Sam told me to learn to write my own material. He said if you steal material, you'll always have a job but you'll never have a career. I never forgot that."
Clark has made a career with a jovial approach that involves a lot of interaction. He makes audiences feel comfortable.
"I have a set list and know what I'm doing, but I love improv, when you see something and react to it," he said. "It's a gift and it's one I don't squander."
He also relates personal experiences and observations, such as this declaration: Women are smarter than men.
"What's a man's best friend? A dog. What's a woman's best friend? Diamonds!" Clark says in his act, before pointing out who will fare better at a pawn shop.
"Women are smarter, they really are," he said. "Guys get mad at me. 'Why do you tell them everything?' Because it's the truth. You can't handle the truth."
Clark said he grew up surrounded by women -- sisters, his mother and grandmother. Still, he cites his dad as his No. 1 comedic influence. "He could take a pencil, lay it on the table, and talk about it and have you laughing before you knew it."
His favorite professional comedian was Red Skelton. He loved how Skelton could make him double over with just his facial expressions. Clark picked up on that; his rubbery, expressive face adds punch to his comedy.
In addition to stand-up, Clark acts in film, TV and commercials. He did a "Law and Order" episode and loved working with Mariska Hartigay in Season 8 of "Law and Order: SVU."
"She was one of the nicest people I ever met in my life," he said. "She was absolutely a doll. She had just had a baby, and she let me hold him while she was getting something done in the make-up chair. She's nice."
So is he. Consider how he closes his shows; it's especially fitting at Christmas.
"Most guys try to end the night with a big laugh," Clark said. "I try to get people to be grateful for what we have. I try to get them to remember where we live and what we have. We've been blessed with a lot."


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PLYMOUTH, MI — The Saginaw Spirit are still searching for their first win on the road.
Saginaw kicked off its 5-game road trip at rival Plymouth in the second of a two-game series with the Whalers, and the Spirit succumbed to a pair of power play goals by the Whalers, including one in overtime for a 4-3 win.
Justin Sefton was assessed a two-minute penalty for slashing with 19 seconds remaining in regulation. Plymouth wasn't able to make it count in regulation, but the Whalers struck 1:20 into overtime on a Zach Lorentz goal for the win.
With the overtime loss, Saginaw moved into a tie with Sault Ste. Marie for first place in the West Division with seven points at 3-4-1-0. Plymouth is in second place at 3-5-0-0 with six points.
Plymouth came out flying at Compuware Arena and took the first lead of the game midway through the first period on a Ryan Hartman goal that Francesco Vilardi followed with a goal of his own a minute later to give the Whalers a 2-0 lead at 9:50 of the first.
Hartman scored his second goal on the power play at the halfway point of the second period to give Plymouth a commanding 3-0 lead.
But the Spirit weren't out.
Captain Eric Locke scored his first goal in his second game since returning to Saginaw that made it 3-1 and started Saginaw's comeback attempt.
Nick Moutrey beat Alex Nedeljkovic for his third goal of the season at 9:17 of the third period, and Sean Callaghan tied it up 3-3 three minutes later.
The Whalers took advantage of their last power play, however, outshooting Saginaw 4-0 in overtime before securing the game winner by Lorentz.
Plymouth finished the game 2 of 4 on power play opportunities, while Saginaw was 0 of 3.
Nikita Serebryakov made 25 saves for the Spirit in his second game of the season.
Justin Kea, Dylan Sadowy and Brandon Prophet had assists for Saginaw, which returns to action Friday, Oct. 11 at Mississauga.
Cory Butzin covers sports for MLive/Saginaw News. Email him at firstname.lastname@example.com or follow him on Twitter.


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Images from Tim Olk of a 2nd Alarm fire at
23072 W Lakeshore Drive in Antioch. The home was near Russell Road in an area protected by the Winthrop Harbor Fire Department. (too many fires … wrong address listed … sorry)
Posts Tagged Winthrop Harbor Fire Department
Images from Tim Olk of a 2nd Alarm fire at
More from Scott Peterson:
Not an inclusive photo gallery but many of the apparatus at Newports 4th alarm.
The Zion Fire Department along with mutual aid companies responded to a fire early this morning at 1817 Kenosha Road. Upon arrival, they found a substantial fire behind the large manufacturing facility located at that address. Thick black smoke filled the air from many tightly packed cardboard bales and containers filled with plastic pellets.
Contributors Tim Olk, Jeff Rudolph, and Larry Shapiro all went to the scene. Tim has submitted several images from rather early in the fire. Images and video will follow from Jeff and Larry.
Tim has more images HERE.
Zion firefighters were assisted by Newport Township, Beach Park and Winthrop Harbor this afternoon when they responded to a house fire at 2408 Ezra Avenue. The vacant house was well involved and has collapsed.
Tim Olk got to the scene right behind the first companies and submitted a few images from his phone.
Tim has a gallery of images HERE.
Police officers reportedly spotted a fire early this morning at the Happyland Pet Store located at 11432 W. Wadsworth Road in Beach Park. First arriving companies found heavy smoke the the alarm was upgraded very quickly.
The Chicago Sun-Times reports that:
The Beach Park Fire Department said firefighters were called to the building that houses the Happyland Pet Center, 11432 W. Wadsworth Road, at 6:06 a.m.
Initial reports said a person was trapped in one of the apartments above the store, but all residents of the building were able to escape the fire.
The Chicago Tribune has an article which states that:
A Beach Park pet store was completely destroyed this morning after a blaze broke out in the basement and killed hamsters, gerbils, fish and reptiles, officials said.
Firefighters were alerted about 6 a.m. to the Happyland Pet Center located at 11432 W. Wadsworth Rd. and found heavy smoke, said Beach Park Fire Department Chief Paul Tierney.
Firefighters went into rescue mode and searched the upstairs of the structure when a flashover occurred. A water supply issue at the scene slowed down firefighting efforts and assisting companies were called in from 15 surrounding communities including Waukegan, Zion and Newport, Tierney said. About 75 firefighters were called to the scene.
"The fire had been burning for quite some time before we were called," said Tierney. He added the fire originated in a concrete bunker-type basement that had only one entrance.
Jeff Rudolph, Tim Olk, and Larry Shapiro all went to the scene. Jeff arrived shortly after the 2nd Alarm was pulled and submitted several images.
A gallery of Jeff's images is available HERE. Tim Olk has images available for viewing HERE. Larry Shapiro has a gallery which can be viewed HERE.
More images and a video will be added later.
Four fire departments in Lake County, MABAS Division 4, have had non-red fire apparatus over the years. To date, only the Fox Lake Fire Department and the Winthrop Harbor Fire Department maintain fleets that are not red, red & white, or red & black.
The fire department at the Great Lakes Naval Training Center in North Chicago used to run apparatus that was either solid lime green or that later had a white roof. The military has since changed over to white over red … with the exception of some ARFF and other airport assigned units.
When the Lake Zurich Rescue Squad and the Lake Zurich Fire Department were separate entities, the rescue squad ran ambulances and a small squad unit. When they purchased this unit in 1979, there was federal funding provided to help with the cost. One stipulation though was that the squad had to be painted white over lime green, which at the time was considered a matter of safety with regards to the visibility of the unit.
The Greater Round Lake Fire Protection District purchased two Mack CF engines; one in 1968 and the other in 1975. Both were painted white.
The Waukegan apparatus has always been red or red and white with a few exceptions. In 1979, they purchased a DOT spec'd small squad just like the one that was bought by the Lake Zurich Rescue Squad. Additionally, they purchased a 1969 Ward LaFrance Model P80 engine on an Ambassador chassis that was white over lime green which was typical of the Ward LaFrance units being shipped during that era. They went on to purchase three more units from Ward LaFrance in 1976 and 1977, all of which were white over red.
Other posts in this series can be found by entering Color of fire trucks in the search field.
Winthrop Harbor sits along Lake Michigan in the northeast corner of MABAS Division 4 along the Wisconsin border. They operate out of one station which is part of the municipal building with the police department. Packed full of apparatus, the four-bay station houses nine pieces of equipment. They are one of the only all Seagrave departments in Illinois with two engines and a 75′ Meanstick quint. Dating back to their long history of FWD rigs, they had FWD logos affixed to all of their modern Seagrave units. All of their equipment with the exception of the ambulances are painted white.
Winthrop Harbor has this restored 1949 FWD engine which is used in parades. The engine made the trip to Clintonville, WI for the Seagrave 125th anniversary.
Jeff Rudolph took in a fire this morning in Winthrop Harbor, in MABAS Division 4 and submitted the following information and photos:
Winthrop Harbor had a box this morning around 0800 for a fire at 11905 12th St. Fire was on the roof of a 1.5 story house, possibly from lightning. Zion Quint 1832 was first in and made the attack. Winthrop Harbor Engine 1711 laid in a supply line. Auto aid was from Newport, Beach Park, and Zion. The box was struck out quickly and companies returned enroute except for North Chicago, and Pleasant Prairie,WI.
As the Chicago area saw it's first snow of the season, area fire departments responded to many accidents. Jeff Rudolph took in a two-car, head-on collision this morning at Route 173 west of Delaney Road in Wadsworth, IL. The Newport Township FPD responded with several units and was aided by Zion and Winthrop Harbor. Six patients were transported with what did not appear to be life-threatening injuries.
Jeff Rudolph recently set up a Flickr site to showcase his photography. His images which include on-scene and static apparatus from more than just the Chicago area can be seen HERE. Jeff has included images that date back many years.


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the MQ errors that you are seeing result from stopping the queue manager
while MQWF is still running.
Always make sure that you first stop MQWF via the admin utility before you
stop the queue manager.
The real problem on your system is the Sqlcode=-811.
I suggest that you
- apply the latest ServicePac (SP 3) for MQWF 3.6
- open a PMR and contact our support team if this does not help.
Your database content might be inconsistent.
Volker Hoss
IBM WebSphere MQ Workflow Development


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Jonathan Chubb
1995, BA, Cambridge University
2000, PhD, University College London
Tel: +44 (0)207 679 7271 (Office)/ +44 (0)207 679 7273 (lab)
Information about Chubb Lab Members
Imaging transcriptional dynamics
Despite the generation of transcriptional differences between nearby related cells being the basis of most differentiation and disease, our standard measures of RNA synthesis do not register the origins of these differences. Although useful for a preliminary rough sorting of genes to context, the widespread techniques of Northern blotting, microarrays, RT-PCR and RNA-Seq measure bulk RNA levels from homogenous population extracts. These approaches lose dynamic information from individual cells, and give the impression transcription is a continuous smooth process. The reality is that transcription is irregular, with strong variable duration periods of activity, interspersed by variable duration periods of inactivity (see movie sequence). Averaged over millions of cells, this appears continuous. But at the individual cell level, there is considerable variability, and for most genes, very little activity at any one time. Transcription of genes, the process which transforms the stable code written in DNA into the mobile RNA message can occur in "bursts" or"pulses" (see movie sequence). These phenomena have recently come to light with the advent of new technologies, to detect RNA in single cells, allowing precise measurements of RNA number, or RNA emergence at a gene. We would like to understand the mechanistic basis of pulsing, and how it is responsive to signals, developmental and chromatin context. And we are testing the implications of noisy transcription on the generation of diversity between cells in developmental and clinical contexts.
Previous posts
2000-2003: Post-doctoral fellow, MRC Human Genetics Unit, Edinburgh
2000-2005: Post-doctoral fellow, Albert Einstein College of Medicine, New York
2005-2011: Principal Investigator, College of Life Sciences, University of Dundee
2012- present: Group leader, MRC LMCB and Department of Cell and Developmental Biology
Selected publications
1. Muramoto T, Cannon D, Gierlinski M, Corrigan A, Barton GJ, Chubb JR. Live imaging of nascent RNA dynamics reveals distinct types of transcriptional pulse regulation. PNAS (in press).
2. Stevense M, Chubb JR, Muramoto T (2011). Nuclear organisation and transcriptional dynamics in Dictyostelium. Dev Growth Differ 53:576-86.
3. Chubb JR and Liverpool TB (2010). Bursts and pulses: insights from single cell studies into transcriptional mechanisms. Current Opinion in Genetics and Development 20:478-84.
4. Muramoto T, Mueller I, Thomas G, Melvin A, Chubb JR (2010). Methylation of H3K4 is required for inheritance of active transcriptional states. Current Biology 20:397-406.
5. Mueller I, Boyle S, Singer RH, Bickmore WA and Chubb JR (2010). Stable morphology, but dynamic internal reorganisation, of interphase human chromosomes in living cells. PLOS ONE e11560.
6. Stevense M, Muramoto T, Mueller I, Chubb JR (2010). Digital nature of the immediate-early transcriptional response. Development 137:579-84.
7. Chubb JR (2009). Gene activation at the edge of the nucleus. EMBO J 28:2145-2146
8. Muramoto T and Chubb JR (2008) Live imaging of the Dictyostelium cell cycle reveals widespread S phase during development, a G2 bias in spore differentiation and a premitotic checkpoint. Development. 135:1647-57.
9. Chubb JR*, Trcek T, Shenoy SM, Singer RH. (2006).
Transcriptional pulsing of a developmental gene. Curr Biol. 2006 16:1018-25.
Awaiting server response...
The Chubb Lab
Lab Members
Danielle Cannon
As an undergraduate I studied pure physics at The University of Dundee. After investigating the structure and formation of amyloid aggregates associated with Alzheimer's disease for my PhD at the Cavendish Laboratories in Cambridge, I moved into Jonathan Chubb's group where I am now using a variety of microscopy techniques to study the single cell gene expression dynamics in Dictyostelium and a variety of mammalian cell types.
Adam Corrigan
am interested in studying the heterogeneity of behaviour in cell
populations, principally the stochastic 'bursts' of transcription
observed in many cells, by developing high-throughput image processing
and analysis techniques and computer simulation.
completed my PhD in biophysics with Prof Dame Athene Donald at the
Cavendish Laboratory, using particle tracking microrheology to study
the formation of protein gels. I developed my interest in measuring
biological phenomena using automated image processing in a short
postdoc with Viji Draviam in the Department of Genetics, University of
Cambridge, studying dynamic spindle orientation.
Agnes Miermont
I completed my PhD at the University Paris 7 where I studied cellular adaptation to environmental variations, and more particularly the response of the yeast Saccharomyces cerevisiae to osmotic stresses. By the combination of lab-on-a-chip devices, molecular biology and microscopy tools, I analyzed how time varying hyper-osmotic shocks influence single-cell response kinetics of signal transduction, compartmentalization and intracellular trafficking.
As I am interested in studying single cell behavior, I joined the group of Jonathan Chubb where I study transcriptional dynamics in Dictyostelium by the use of high-resolution live imaging combined with state-of-the-art image analysis.
Page last modified on 26 sep 14 12:25


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Proteins may undergo glycosylation by enzymatic or nonenzymatic mechanism.1 Nonenzymatic glycosylation of proteins can occur under physiological conditions. Nonenzymatic glycosylation of hemoglobins (Hbs) results in the formation of Hb A1a, Hb A1b, and Hb A1c, which are collectively known as glycosylated hemoglobins. Hemoglobin A1c is the Amadori reaction product of the N-terminal valine of each β-chain of Hb A with glucose.2
Hemoglobin A1c has been extensively used as a measure of metabolic control in diabetic patients.1 The degree of control of hyperglycemia as evaluated by the concentration of Hb A1c in blood has been found to correlate with the occurrence of diabetic complications, particularly vasculopathy.3 Brancati,4 in an excellent, thought-provoking editorial in the April 10, 1995, issue of the Archives discussed many aspects of the benefits of tight glycemic control in diabetes mellitus. However, the role that Hb
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The City Council is likely to approve on Monday a 5 percent tax on resident's gas bills, a measure that will close a nearly $600,000 shortfall in the 2013 budget and leave a substantial amount to use on capital improvement projects.
With recent action being taken to forego levying an electric bill tax and keep property tax levels steady, city council members will go into Monday's council meeting with a decision to either raise revenue or continue cuts to residents services or even scale back city workers.
At a special budget meeting last week, City Manager Todd Thompson said it is possible that trimming staff could be in the mix if the city does not raise revenue needed to close its $600,000 shortfall. Personnel expenses make up 70 percent of the city's budget, he said.
"Given the scope of the cuts the city has made over the years, you're at the point where cuts are gonna impact service levels," Thompson said at the budget meeting. "You have to ask yourself, at what point does the city become part of the economic problem if we let people go? We do have services to provide."
City administration has recommended the council approve the 5 percent tax on gas bills, which would go into effect Feb. 1. The tax would generate $770,000, enough to close the budget gap and fund other community projects, such as sidewalk upgrades. The council originally considered levying a gas tax of 2 percent, before the council voted down the electricity tax and decided to keep property taxes steady. A 2 percent gas tax would generate $340,000.
Based on levels of support voiced at previous meetings, it is likely the measure will pass the council, possibly by a 5-2 vote.
Council members weighed in on the gas tax issue at the recent budget meeting, with three council members indicating their support for the extra revenue. Council members Corine Andersen, Ward 4, Peter Schwartzman, Ward 5, and Jeremy Karlin, Ward 7, said they would back the proposal in an effort to keep city services intact.
Members Wayne Dennis, Ward 2, and Wayne Allen, Ward 6, said they needed additional time to consider the proposal. Dennis said he is likely to support the measure if no other tax increases are considered. Allen said he echoes that sentiment.
"It will probably get approved, but I still think we should make some cuts," Dennis said. "I think the city should make some cuts before we go through it. We can't spend money we don't."
Dennis suggested that the city keep some positions unfilled after a personal retires or leaves, and that privatization of some city facilities be considered.
Aldermen Ken Goad, Ward 1, and Russell Fleming, Ward 3, said they are unlikely to support the gas tax. Fleming said he would prefer to see the city nix some projects in next year's budget, for example a planned upgrade to an Oak Street facilities building. Goad said the community cannot afford the raise.
Page 2 of 2 - "You may all think that's unrealistic," Goad said of his desire to keep taxes flat. "But I think it's unrealistic of the city to spend more money and ask more from the citizens. We have people struggling, and we have things in this budget that are non essential."
The local Chamber of Commerce has said it will be at Monday's council meeting to share with the city its findings from a survey of chamber members on how they'd like the city to cover the budget shortfall.
Executive Director of the chamber, Steven Brody, told The Register-Mail his group sent out a survey to its members, and that the response has been robust.
"(City Council) members asked for input," Brody said. "I took that as 'let's do it.' This is our opportunity to help the city out."
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Location, ST | website.com


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Cleaning up McCarty's Cove
November 1, 2015 MARQUETTE — It didn't take long for North Star Academy students to start filling their small plastic bags with cigarette butts from McCarty's Cove on Friday. more »»
Memorable moments are often the strange ones
November 1, 2015 In my family we like traditions, and two years ago, we started a new one. Two years ago, in the early spring we went to Mackinac Island for the first time. more »»
Saying farewell: The final time on the field
October 25, 2015 I will be doing something this week that I never fully realized I would be doing. more »»
Cold Logic
October 25, 2015 MARQUETTE — Multi-colored lights and moving parts are the flashier parts of the teen robotics world. The more subdued aspects involve fundraising so participants can actually make the robotics. more »»
Cat Pack fever
October 18, 2015 MARQUETTE — Not everyone gets emotional over a peanut butter and jelly sandwich. It also can be embarrassing to have a loud rumbling stomach in the middle of quiet classroom time. more »»
Amazing Grace: Missing a special friend
October 18, 2015 Have you ever had a person in your life that you love because they made you feel happy and special? I was lucky because I had someone who did. Grace McCarthey was born on July 26, 1920. more »»
Say what? Teenage Slanguage
October 11, 2015 About 3,000 years ago, some cave man thought it would be fun to rub two stones together to see what would happen, and voila, he created fire. more »»
October 11, 2015 CHATHAM — A little modeling clay and some toy cars, and kids are all ready to learn about science. more »»
The wonderful world of Scratch
October 4, 2015 What would you say if I said to you that I can create an advanced coding system, such as Minecraft, in a mere hour or so? This sounds hard, right? "Advanced coding systems." Sheesh. more »»
BIBLES and bean bags
September 26, 2015 MARQUETTE — Sportsmanship and Scriptures don't have to be mutually exclusive. The weekly Awana program at Bethel Baptist Church, located at 829 W. Grove St. more »»
Adjusting to life as an MSU Spartan
September 26, 2015 As you read this column, I am in East Lansing getting ready for my fifth week of school as a Michigan State University Spartan. more »»
September 20, 2015 MARQUETTE — An anti-bullying message can apply to anyone regardless of age. But when it's being presented to middle schoolers, a little fun is in order. more »»
Why America feels different than France
September 20, 2015 My name is Luna. I live in France, and I'm going to tell you about some of the differences I can see between our two countries. First, the food is totally different. more »»
MILLENIALS: Self-absorbed, wasteful?
September 6, 2015 WASHINGTON — Even millennials don't think much of their generation, according to a new poll Thursda. more »»
Back-to-school blues: Pakistan
September 6, 2015 Aug. 12, 2015, for some in Karachi, Pakistan, was just another date. For many others it was a new beginning. more »»
American food is plentiful, fast and pleasant
August 30, 2015 I'm going to talk about American food. First of all, American breakfast. It's very substantial. The first time I had breakfast in the States, I didn't know how I was going to eat it all. more »»
Teens are shopping more like their parents during back-to-school season
August 30, 2015 By ANNE D'INNOCENZIO AP Retail Writer GARDEN CITY, N.Y. — Giulia Pugliese is a typical teenager. She likes to look good, and she's particular about what she wears. more »»
Students learn important role winged critters play
August 16, 2015 MARQUETTE—Children and parents at the Upper Peninsula Children's Museum Wednesday morning attended a special presentation by Christina Funk, lead education specialist at the Organization for Bat... more »»
News, Blogs & Events Web


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SBOE Blocks Great Hearts Charter School Expansion
The Texas State Board of Education on Friday denied an Arizona-based charter school's request for a Dallas campus, despite previous approval from the state's education commissioner.
The 15-member board voted 9 to 6 to veto Great Hearts Academies' application because of concerns about the school's commitment to serving low-income students and teaching Texas curriculum standards.
"I have no confidence, really, in the Great Hearts organization," said board member Mavis Knight, D-Dallas, who has led opposition to the school.
This is the first charter application cycle under a law passed during the 2o13 that shifted the primary responsibility ...


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Scudder Falls Bridge
In 2002, the DRJTBC completed the Southerly Crossings Corridor Study, which recommended certain improvements at the Scudder Falls Bridge and along portions of I-95 to relieve current and future congestion and to improve safety. The study concluded that:
The Scudder Falls Bridge currently carries about 55,000 vehicles per day and operates at the worst level of service (LOS F) during peak rush hours in the morning and afternoon. A level of service F means that it operates under the most extreme conditions of congestion. By 2030, traffic volumes are expected to increase an additional 35% to about 74,000 vehicles per day further increasing congestion.
|Within a half-mile of the Scudder Falls Bridge there are two interchanges that have substandard (by today's standards) geometric features
In January 2003, the DRJTBC signed a Memorandum of Agreement with PENNDOT and NJDOT to proceed with the environmental studies and preliminary design for the I-95/Scudder Falls Bridge Improvement Project. These organizations agreed to use PENNDOT's Project Development Process to fulfill the National Environmental Policy Act (NEPA) process requirements.
COMMISSION APPROVES SCUDDER FALLS BRIDGE TOLL SCHEDULE, TOLL ADJUSTMENT FOR RVs TOWING TRAILERS OR CARS


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EAST AMWELL TWP. — For the third time, Jammin' for Jersey has assembled its musical friends and attracted an audience to raise money to ease the ongoing plight of Hurricane Sandy's victims. This time it was an event called "Cinco de Vino" and it was held Sunday, May 5, at the Old York Cellars winery in East Amwell Township.
The next fundraiser will be held at Circa in High Bridge sometime in August.
"I don't think anyone realizes there's still a problem" at the Jersey Shore, said Jammin' for Jersey president Sophia Fleming of Stewartsville, standing on the terrace at the winery on Sunday afternoon. "We have power and the sun is shining, but it's going to take a long time to restore" the property that was damaged and the lives that were disrupted,she said.
Inside, Robert Stephen Viola of Raritan Borough was singing and playing his acoustic guitar. Fleming said that she met Viola when she was a teenager competing for a rural royalty crown at the Warren County Farmers' Fair. He accompanied her on guitar while she sang a song, and she's known him ever since.
When Jammin' for Jersey was created, connections like that formed the core of the organization, which networked among friends to recruit performers.
Fleming describes Jammin' for Jersey as "kindred spirits pulling together. People who have felt hardship" seem to be especially eager to embrace the cause, she said. In her own case, she remembers the feeling of losing her home and belongings to a fire when she was a young girl.
The other organizers are Mike Frank, Joan Pacifico and Jena Ardell, all of Stewartsville, and Mike O'Brien of Pattenburg.
Besides Viola, Frank and O'Brien, the performers included Gregg Cagno, Joe Cassady, Brian Molnar, Mike Massimo, Christian Roebling and his daughter Sofia Zul, and Ellyot Ray.
Old York Cellars donated the use of the premises and gave a percentage of wine sales to the cause.
Jammin' for Jersey is helping the Yurgelonis family of Matawan, which has 11 kids ages 34 to 7. After rebuilding their home after a fire five years ago, "Superstorm Sandy came and completely took the whole three-floor home, leaving them homeless once again with no means to rebuild," says the Jammin' for Jersey website.
For more information, contact firstname.lastname@example.com or visit the website.
More Hunterdon County news: NJ.com/hunterdon • Facebook • Twitter


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In less than 30 minutes we were on the ship. Surprisingly, once on the Dream we were allowed to proceed to our cabins. Our cabin was ready and clean and we were greated by our cabin steward Sparky who was cheerful and extremely helpful through our cruise. I found all the crew members to be pleasant and cheerful.
Our dining room experience was pleasant. We had a group of eight and were accommodated nicely in the Crimson Dining Room.
I must say that after reading the reviews about the horrible smell on the Dream I was a little worried. Although I did smell it on several occasions, it wasn't that bad, a brief swiff of sewer that went as fast as it came.
The lines in the public food areas were a little long, but that's to be expected with a ship this size trying to accommodate over 3,000 people. The food was ok, about what I expected and the same as on other Carnival ships.
Disembarkation was well organized and we were off the ship in no time with no waiting. I am glad Carnival revamped the Muster Drill process to have it in an air conditioned area without having to lagg the life vests around.
The worse part of the entire week for me and my group was the lack of supervision of all the children that were onboard. We were awakened several nights by what sounded like thunder in the halls, and turned out to be children, including young children who were unsupervised and running (literally) all over the ship. On one occasion I had to come out of my cabin to ask the gang of about 5 young boys to please stop running. I can't believe that people give their kids a Sail & Sign card and just let them go wherever they want, whenever they want.
That being said, overall, the cruise was great and I look forward to cruising with Carnival again.


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25 July 2006
Volunteer at the UNHCR refugee settlement in Ghana
Buduburam, Ghana
The New Liberian Women's Skills Training Centre at the UNHCR settlement gives financial support, advice and training that enables women to be self-reliant and live as normal a life as possible. Their core areas are:
• Practical Skills training including sewing, baking, soap science, masonry, and computer science.
• Micro credit loans enabling graduates from the training centre to set up their own small-scale business.
• Information sessions on women's rights, particularly focused to education of sexual gender based violence (SGBV)
We are looking for a range of volunteers, for more information please visit our website www.ikando.org


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- 08
- Aug
From foot licking to dildo play, sexy babes Heather Starlet and Veronica Ricci love exploring and enjoying each other's hot body.
From foot licking to dildo play, sexy babes Heather Starlet and Veronica Ricci love exploring and enjoying each other's hot body.
Veronica Ricci's beautiful wet, naked body glistens in the sunlight as she poses on the steps leading to a botanical garden.
When 2012 Penthouse Pet of the Year Jenna Rose hooks up with November 2009 Pet Veronica Ricci, the photographer barely has time to setup before these two hot women are all over each other.
April 2009 Penthouse Pet Veronica Ricci spices up her warm bath with a long session of fingering and rubbing.
Penthouse Pet Veronica Ricci shows off a bit of her wild side and a lot of her gorgeous naked lily white ass while modeling black lingerie on top of a zebra skin rug.
Penthouse Pet Veronica Ricci is absolutely radiant posing naked in her white living room with the bright California sun streaming in onto her, softening her sexy curves and haloing her bright red hair.
Penthouse Pets Taylor Vixen and Veronica Ricci stage their own backyard production of lesbian theatre. Get comfy and watch these two sexy ladies perform on each other all night long.
She's just too damn hot! Veronica Ricci quickly sheds her pink faux fur and bares her creamy busty body by the pool.


text: Cited by Stephen King as his favorite TV show of 2012, the blockbuster political drama Borgen explores the insular world of high-stakes Danish politics and the press corps that covers it in instantaneous, relentless news cycles. When Birgitte Nyborg becomes Prime Minister through a political fluke she has to quickly learn the ways of power. Over the course of three seasons, through the ups and downs of a life both inside and outside of public office, can she maintain her politics and her integrity? 2010-2013. In Danish with English subtitles. The complete series is available on DVD at http://shop.mhznetworks.org.


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Craftsman 1/4 x 56-7/8 in. Band Saw Blade, 15TPI, Regular Tooth
$1.93 - $2.93
Store pickup
1 year parts and labor
Give Your Band Saw Some Teeth with the Craftsman 1/4 x 56-inch Regular Band Saw Blade This quality Craftsman-1/4-x-56 7/8-in. band saw blade features 15 teeth per inch, making it perfect for wide-scrolling projects where you need fine detail and a smooth finish. Band saws make a variety of cuts, but you must have the right blade to produce a smooth finish on any cut. Keep this blade in your shop or art studio so you can always complete the project at hand quickly and easily. Craftsman's quality blades are sharp and designed to deliver pristine cuts, helping you achieve a professional look. Equip your band saw with the Craftsman 1/4 x 56-inch regular band saw blade to achieve a professional look for all wide-scrolling projects.
- Craftsman-1/4-x-56 7/8-in. band saw cuts through any material with minimal effort
- 15 teeth per inch
- General smooth blade for wide scrolling
Specifications & Dimensions
Product Documentation
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Callaway X Hot Pro Driver
NOW: $134.97 (55% off!)
Was: $299.99*
Product Information:
Scorching hot distance. Adjustability and Forgiveness. The Callaway® Men's X Hot Pro Driver is designed for maximum distance with advanced adjustability and forgiveness. Featuring an overall lighter configuration to deliver hotter ball speeds and powerful trajectories for a wide range of swings on any course.
- OptiFit® Technology - Hosel adjusts the face angle in open, square, or closed positions to improve accuracy and trajectory and allows golfers to dial in their preferred look at address
- Speed FrameT Face Technology: A combination of VFT and Hyperbolic Face Technology creates a larger sweet spot and fast ball speed for maximum distance. This technology also saves weight that can be redistributed to the perimeter of the clubhead for a higher Moment of Inertia (MOI)
- Ultra Thin Wall Casting Technology: Thinnest casting technology ever creates a lighter overall configuration and an optimized CG to promote more distance
- Lower club weight and swing weight for improved feel
- Lightweight Project X Velocity Shaft: This premium lightweight shaft further enhances speed and distance
- Callaway
- Loft: 8.5°
- Lie: 58.5°
- Length: 45.8"
- Face Angle: Adjustable
- Head Weight: 198g
- Volume: 435cc
- Swing Weight: D3
- Loft: 9.5°
- Lie: 58.5°
- Length: 45.8"
- Face Angle: Adjustable
- Head Weight: 198g
- Volume: 435cc
- Swing Weight: D3
- Loft: 10.5°
- Lie: 58.5°
- Length: 45.8"
- Face Angle: Adjustable
- Head Weight: 198g
- Volume: 435cc
- Swing Weight: D3
Country of Origin: ImportedWeb ID: 19061896
Customer Reviews:
Rated 4.6 out of 5�by 38 reviewers.
Rated 4 out of 5�by dgs808 As Long as Advertised Easy 10 yards longer than my old Callaway Hyper X Pro, and that was plenty long. Be careful buying online. Didn't come with hosel tool. February 12, 2016
Rated 5 out of 5�by daveeagle Driver Replaced my Cobra Baffler with this new Callaway. Added more yardage and hit straight and true. Good quality and a very good price. July 15, 2015
Rated 4 out of 5�by ColinT Longer and straighter This is my best driver since my XR03 got banned. Lets hope it lasts. May 5, 2015
Rated 4 out of 5�by porkey The product drives the ball well The driver Drives the ball real well. It is very light and easy to swing. It is great. February 17, 2015
Rated 5 out of 5�by Big Dog Awesome Love how the ball pops off the face! I have regained lost distance and its a great feeling! September 23, 2014
Rated 5 out of 5�by magicman361 great driver for the price Very large sweet spot. Got cut to fit. Overall performance very good. Avg drives 250 to 275 yrds September 16, 2014
Rated 5 out of 5�by JSTORM Straighter shots off the tee!!! I'm a new player that has been struggling with a caddyshack worthy slice. Went into the store looking for some relief from it and now almost every tee shot is right down the middle. The adjustable head has made all the difference in the world and I have shaved off a few strokes in my game. I was mid 90s on par 72s and now I'm mid to low to mid 80s due to a better positioned second shot. A hair shorter distance wise than the other club I was using, but not enough to make a game changing impact. I also enjoy the feel of the ball when it connects!!! Hardly feel a thing. I would recommend this product to anyone that is looking to straighten out their shots!!! September 10, 2014
Rated 5 out of 5�by Flip Increased distance immediately. I did, however, put a new, oversize, Winn grip on it. August 13, 2014
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