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Gastric bypass surgery, also called the Roux-en-Y gastric bypass procedure, is the most common form of weight loss surgery performed in the United States today.
Also known as stomach bypass surgery or bariatric bypass surgery, this procedure creates a small pouch that bypasses the stomach and attaches to the intestine. A gastric bypass operation is irreversible, in most cases.
There are two types of gastric bypass surgery performed at UPMC. Your bariatric surgeon will perform gastric bypass surgery either:
Here's what to expect if you're considering a gastric (stomach) bypass surgery procedure.
After you receive general anesthesia, your bariatric surgeon will:
Those who are severely overweight and cannot lose weight through diet and exercise may turn to gastric bypass surgery.
Undergoing gastric bypass surgery is a serious commitment to a healthier lifestyle — patients must:
Doctors may perform this procedure on patients with a high Body Mass Index as well as obesity-related health conditions.
Find out more about the benefits of weight-loss surgery.
People who qualify for gastric bypass surgery include:
Learn how to calculate your own Body Mass Index.
Dumping syndrome is a potential risk of gastric bypass surgery. It occurs when large volumes of food in the stomach move too quickly through the small intestine, frequently after eating sweet or high-fat foods.
Dumping syndrome can cause:
Other gastric bypass surgery risks and complications include:
Your bariatric surgeon will review all potential gastric bypass surgery risks, complications, and other weight loss surgery options with you prior to your procedure. If you have any questions about gastric bypass surgery, we encourage you to ask your surgeon.
Learn more about what to expect after gastric bypass surgery.
Affiliated with the University of Pittsburgh Schools of the Health Sciences
Supplemental content provided by Healthwise, Incorporated. To learn more, visit www.healthwise.org
For help in finding a doctor or health service that suits your needs, call the UPMC Referral Service at 412-647-UPMC (8762) or 1-800-533-UPMC (8762). Select option 1.
UPMC is an equal opportunity employer. UPMC policy prohibits discrimination or harassment on the basis of race, color, religion, ancestry, national origin, age, sex, genetics, sexual orientation, gender identity, marital status, familial status, disability, veteran status, or any other legally protected group status. Further, UPMC will continue to support and promote equal employment opportunity, human dignity, and racial, ethnic, and cultural diversity. This policy applies to admissions, employment, and access to and treatment in UPMC programs and activities. This commitment is made by UPMC in accordance with federal, state, and/or local laws and regulations.
Medical information made available on UPMC.com is not intended to be used as a substitute for professional medical advice, diagnosis, or treatment. You should not rely entirely on this information for your health care needs. Ask your own doctor or health care provider any specific medical questions that you have. Further, UPMC.com is not a tool to be used in the case of an emergency. If an emergency arises, you should seek appropriate emergency medical services.
For UPMC Mercy Patients: As a Catholic hospital, UPMC Mercy abides by the Ethical and Religious Directives for Catholic Health Care Services, as determined by the United States Conference of Catholic Bishops. As such, UPMC Mercy neither endorses nor provides medical practices and/or procedures that contradict the moral teachings of the Roman Catholic Church.
Pittsburgh, PA, USA UPMC.com


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Quote from: tnargs on March 19, 2013, 09:34:41 AMThe weight is in the panasonic G3 class - amazing. And the lens mount looks h-u-g-e!Yeah, here's a comparison to the other smallest Rebels of the past (XTi and XS), sorry I was too lazy to resize the pictures:[...]EDIT: On the opposite side of the spectrum, here it is in comparison to the EOS M and EF adapter:[...]
The weight is in the panasonic G3 class - amazing. And the lens mount looks h-u-g-e!
yet another disappointment from canon. old sensor, order specs, outrageous price. why can't they just accept the future of the sub 1000 camera body is mirror-less. enough with this surplus parts revel crap.
Really, sometimes I think the only thing that'll satisfy the peanut gallery 'round here is if the camera gets delivered by an invisible flying pink unicorn pony -- and one that grants sexual favors, to boot....b&
edit: deleted, I got the D3200 weight wrong, this is actually about 15% lighter than a D3200
So the 6D has a sync speed of 1/180th while this toy camera could go up to 1/200th? If that's true I really don't get what Canon is doing with the specs of their semi-pro/pro cameras…
Quote from: NormanBates on March 19, 2013, 12:35:48 PMedit: deleted, I got the D3200 weight wrong, this is actually about 15% lighter than a D3200It's also significantly smaller than the D3200. The D3200 is basically the same size as the T3; the EOS-b is a lot smaller.Cheers,b&
Well, keep in mind that lower-end cameras sell a lot more than higher-end cameras and that if you intend to get people to move up to higher-end cameras, it's a lot easier to convince them if they already have one of your cameras (brand loyalty).

subset: commoncrawl


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State Sen. Leland Yee, D-San Francisco, is not the only California lawmaker to receive hostile messages from people opposing gun control legislation.
Sen. Kevin DeLeon, D-Los Angeles, told KCRA 3 that he has received phone calls and faxes from people as far away as Virginia, Alabama and Louisiana attacking him with ethnic slurs following his introduction at the beginning of the year of a bill that would require background checks for people buying ammunition.
"I've had some really mean-spirited faxes," DeLeon said. "Faxes like, 'Why don't you regulate taco trucks instead of guns?' But one [that] was a little disturbing was, 'I hope you die of a crime.' "
DeLeon said another fax -- also in response to his ammunition legislation -- read, "Go back to Mexico, illegal alien."
DeLeon has not received direct threats, but Yee has.
The California Highway Patrol believes the threat was made by Everett Basham, a man who was arrested Tuesday in Santa Clara.
Investigators found illegal weapons and illegal bomb-making substances there, according to Chief of Protective Services Scott MacGregor.
Yee said the threat detailed how its sender would kill him and said the person knows where Yee's office is at the state Capitol.


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People with celiac disease who eat a gluten-free diet experience fewer symptoms and complications of the disease. People with celiac disease must eat a strictly gluten-free diet and must remain on the diet for the remainder of their lives.
In some severe cases, a gluten-free diet alone can't stop the symptoms and complications of celiac disease. In these cases, doctors might prescribe medications to suppress the immune system.
Not getting enough vitamins
People who follow a gluten-free diet may have low levels of certain vitamins and nutrients in their diets. Many grains are enriched with vitamins. Avoiding grains with a gluten-free diet may mean eating fewer of these enriched products. Ask your dietitian to review your diet to see that you're getting enough of these key nutrients:
- Iron
- Calcium
- Fiber
- Thiamin
- Riboflavin
- Niacin
- Folate
Not sticking to the gluten-free diet
If you accidentally eat a product that contains gluten, you may experience abdominal pain and diarrhea. Some people experience no signs or symptoms after eating gluten, but this doesn't mean it's not damaging their small intestines. Even trace amounts of gluten in your diet may be damaging, whether or not they cause signs or symptoms.
Source: http://www.mayoclinic.org/healthy-living/nutrition-and-healthy-eating/in-depth/gluten-free-diet/art-20048530/


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Talk about an eventful (belated) birthday! Pink and her husband Carey Hart celebrated her 31st with a camping trip at Cachuma Lake, near Santa Barbara, Calif. The couple rode around on motorcycles and spent the evenings in front of a campfire. "They seemed to have an amazing weekend," an eyewitness said. But did they celebrate too much? After complaints they played music too loudly, the two were asked to leave the campground – not that Pink seemed bothered. "F--- it all," the singer later Tweeted. "I love camping. We are the only couple I know that can get kicked out a campground."
Eight-time VMA winner Lady Gaga kick started her big weekend with a group dinner at WP24, Wolfgang Puck's new restaurant in L.A. The singer, in a sheer plastic dress over black lingerie, sat in the middle of the busy dining room and tucked into Chinese-inspired dishes off the prix fixe menu. Meanwhile, Usher and best new artist winner Justin Bieber celebrated post-VMAs with a meal at restaurant.
Host Chelsea Handler continued her night at Teddy's inside the Hollywood Roosevelt Hotel with a bash deejayed by nominee Florence + the Machine. Guests, like Aziz Ansari and Ronnie Ortiz-Magro and Sammi Giancola, milled about Teddy's and the hotel lobby, though the Jersey Shore stars didn't seem to interact with each other.
Johnny Depp made a trip to Paris for the weekend to hang with partner Vanessa Paradis and their kids, Lily-Rose and Jack. The family lunched at Brasserie Lipp, and Depp snapped pictures with two lucky fans as they left.
By PERNILLA CEDENHEIM, JENNIFER GARCIA and PETER MIKELBANK
Caught in the Act!


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It's no secret that the relationship
between Apple and Google is a bit
rocky to say the least. While the two companies forged a
partnership with the launch of the original iPhone (Google makes its
presence known on the iPhone via Mobile Safari search, YouTube app,
and Maps app), Steve Jobs over the past six months or so has been
growing a bit resentful of Google's rise to power with its Android
mobile operating system.
However, Google's Larry Page now
contends that Jobs'
anger is misplaced. While speaking at the Allen & Co
conference this week in Silicon Valley, Page stated, "We had
been working on Android a very long time, with the notion of
producing phones that are Internet enabled and have good browsers and
all that because that did not exist in the marketplace."
"I think that characterization of
us entering after is not really reasonable."
Page then went on to say that Jobs'
characterization of the events that took place leading up to Google's
release of Android OS as "a little bit of rewriting history."
Earlier this year, Steve Jobs went on a
at an Apple Town hall stating that Google's "Don't Be Evil
Mantra Is Bulls**t" -- another source contends that Jobs said
that "a load of crap" was the actual statement used, but
you get the point.
Last month at the All Things D
conference, Jobs again touched on the Google subject stating, "They
decided to compete with us. We didn't go into the search business!"
Despite the difference of opinion on
Google' motives for Android, Google CEO (and former Apple board
member) Eric Schmidt also weighed in stating that the two companies
still have a strong partnership with the iPhone. And just this past
March, Jobs and Schmidt were seen canoodling
outside a cafe in Palo Alto, California.
quote: Actually, it was Palm that made the Treo.. My Treo ran Palm OS, it was a Palm device... As much as the iPhone is an Apple device, and not Foxconn's. You're missing the entire point however I fear.
quote: Palm never sued anyone for releasing apps for their hardware.. It was encouraged. The strength in the Palm OS scape, that is sadly neutered with the iPhone with Apple controlling all, is that I didn't have to hack my device to install any 3rd party app I wanted.
quote: Also Apple doesn't make every app for the iPhone, it is 3rd parties that have made the App Store as good and bad as it is. So are we then saying these 3rd party apps can't be counted as part of the iPhone experience?
quote: The iPhone is an iPod with a GSM radio and wifi... so yes naturally the music integration is wonderful, however that does not a smart phone make.
quote: All I am saying, is that allowing for hardware changes over the past 5-6 years my PALM Treo 650 could do everything the iPhone does, and more. The iPhone does it faster, prettier, with a larger screen, and has a far better browsing experience. HOWEVER: 5+ years later in this market segment? It damn well should!
quote: Besides which... cut and paste and tethering came standard with my treo 650 in like 2005/6... I had to wait for various firmware upgrades from Apple before my iPhone 3G could do it in 2009. Srsly? Sad.
quote: No, you are missing the point, because Handspring made the Treo and the whole expansion scheme.


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Grass-Fed Leg of Lamb, Bone-In
Product Actions
Note: Frozen items may thaw in transit.
Note: Frozen items may thaw in transit.
This bone-in leg of lamb is proof that meat really does taste best when roasted on the bone. It's a classic preparation, and easy to do.
Just rub with fresh rosemary and garlic for savory and herbaceous flavor. Pop the lamb in the oven and baste a few times. You'll end up with a gorgeous piece of meat that will draw oohs and aahs.
But the real pleasure comes when you eat it. Mild and meltingly tender, each mouthful is a revelation.
Drink a full-bodied red wine like Australian Shiraz, or if you prefer craft beer, try Double IPA or Scotch ale to complement the full-flavored roast.
Our goal in all we do is to provide healthy and delicious meat that comes from a clean and sustainable environment. We are dedicated to finding ranchers and farmers that share our vision of a more humane and sustainable way of rearing livestock. That's why we work only with those who respect nature and focus on the best animal welfare practices.
D'Artagnan sources lamb from Australia, where there is a strong tradition of pasturing these woolly ruminants. Our humanely-raised lamb comes from a clean and natural, free-range environment where the young animals graze on grasses like rye and clover in spacious pastures and semi-arid rangelands.
Most importantly, when lambs are raised this way there is no need for antibiotics to prevent disease, as is common in crowded feedlot conditions. Our lambs are never, ever administered antibiotics, hormones or any other unpleasant substances.
The Border Leicester-Merino, Dorset and White Suffolk cross-breed sheep are raised to maturity in 6 months, instead of the more typical 9-12 months. The idyllic growing conditions, low-stress environment and the young age of the lambs all ensure a clean, mild-tasting meat that is never gamey.


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We're here to help: 888.811.5053
Our Sleep Experts can answer your questions, help place your order and help you find your best night's sleep!
Mon-Fri 8am-11pm EST
Sat-Sun 9am-6pm EST
For questions after you buy or changes to your order
Mon-Fri 8am-8pm EST
Sat 8am-5pm EST,
Closed Sunday
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We're passionate about better sleep. It's what drove the original "Swedish scientists" who spent years perfecting a NASA® invention into TEMPUR material. It's the force that guided our founder, who wanted to make TEMPUR mattresses available to people in the U.S. and across the globe. And it is the passion that drives us today, as we create new and better sleep products that continue to change the way the world sleeps.
We're passionate about controlling the quality of our products. So passionate that we built two state-of-the-art production plants, one in Duffield, Virginia and another in Albuquerque, New Mexico. We control our own production lines, so we can ensure that the mattresses and pillows we produce are of the highest quality.
We're passionate about doing things the right way, even if we upset the status quo. We completely did away with traditional springs in our mattresses in favor of a radical new material that was completely unknown at the time. We work hard to ensure our pricing is consistent no matter where you buy, so you're not pressured to buy only for a cheap price. We warranty all of our larger products because it's the right thing to do (and because we know they'll last a long time).
We're passionate about a great delivery experience. When customers order a mattress and foundation or Ergo adjustable base, they all receive our White Glove Delivery Service. We deliver the mattress, set it up, and even take away the old mattress.*
We're passionate about the right mattress fit. We offer both a website and Sleep Experts to help you select the right product. And we offer our 90 Night Tryout, which helps assure you'll be happy with the mattress you bought direct from us.
We're passionate about the durability of our products. That's why we offer warranties on all of our larger products—many unheard of in the industry. A 20 year limited warranty on mattresses and Ergo adjustable bases. A 3 year limited warranty on pillows and cushions. Even a 1 year limited warranty on bed linens.** We stand behind our products.
*Standard delivery fees apply.
**See warranty language for details.


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: myInterpreter begin 32 word find dup if execute else number then again ;And here would be a compiler:
: my-] begin 32 word find dup if dup immediate? if execute else compile then else compile-number then again ;A word such as [ would be an immediate word, which manipulates the return stack (or in ANSI-like dialects, would change the state of a variable imaginatively called STATE) to break out of the compiler loop, while a word like ] usually is the compiler itself. This preserves Forth's [ and ] semantics. Of course, you'll also need to re-implement : and :NONAME as well, but these are rather trivial. For a concrete example, here's how to change the compiler so that all words prefixed by the back-tick are POSTPONEd:
\ not tested code; but it would look/feel a lot like this. create macro-buffer 80 allot : create-postpone-macro S" POSTPONE " macro-buffer 1+ swap move ( embed the "POSTPONE " part into the buffer ) count dup -rot macro-buffer 10 + swap move ( embed the name of the word after POSTPONE ) 9 + macro-buffer c! ; ( and set the length of the whole string. ) : word-starts-with-`? dup 1+ c@ [char] ` = ; : new-] state on begin 32 word find dup if dup 0< if execute ( it was an immediate word ) else compile ( it wasn't an immediate, so compile it instead ) else word-starts-with-`? if compute-postpone-macro macro-buffer count evaluate else number literal then then state @ 0= until ; : : (:) new-] ; ( most Forth systems have a word like (:), that implements the core of : without actually invoking the compiler ) : ] new-] ; immediate ( note this word compiled with the "new" :-compiler! )And that should be it. Yeah, it's a bit more complex than just creating a reader macro in Lisp, but hey, when the whole thing compiles to maybe 300 bytes or less on a 32-bit system, you could afford some 15 of these things in memory before you even hit the complexity of the reader itself, let alone the interpreter and compiler logic. :-) (Not sure who changed the ] to [ characters. In Forth, [ is used to enter into interpreter mode from inside a definition. This allows compile-time pre-computed constants with zero run-time overhead, like this: : abc blah [ 2 4 * 5 6 * + ] literal blort ;. Hence, ] is the entry-point to the compiler, NOT [.) --SamuelFalvo?
def python_interpreter(code): exec code def ruby_interpreter code eval code endHow is this any different from implementing a Lisp interpeter using Lisp's eval? --DavidMcLean?


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Elkins High School, Midland Elementary evacuate
March 20, 2013
Both Elkins High School and Midland Elementary have been evacuated following a bomb threat at the high school....
Breaking news is broken!
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At 9:42am this morning, I received a call from "Randolph County Schools" stating that schools will dismiss at 9:45am. Parents need not come pick up their children however. Your breaking news is a little outdated.
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CLEMSON — Asked to recall the last time he led such a young team, Jack Leggett paused prior to answering, mentally paging through the teams he has led in 20 seasons as a Division I coach.
"There's some newness and some question marks," the Clemson coach said, "there's no question about it."
Clemson enters its 2013 season Friday against William & Mary with depressed expectations – unranked in national polls and picked to finish third in the Atlantic Division in the coaches' preseason poll – due to the youth and unknowns populating the roster.
While former stars like Richie Shaffer, Spencer Kieboom and Kevin Brady will be in professional camps this spring, Clemson will be seeking answers.
The concerns mostly center on the everyday lineup. The Tigers, an NCAA team a year ago, return only three everyday players in second baseman Steve Wilkerson, center fielder Thomas Brittle and first baseman John McGibbon. Not only will the lineup have a new look, but it also figures to have produce runs in new ways with Shaffer among the departed players who combined for 36 of the team's 44 home runs last season.
The loss of Shaffer cannot be understated. Former South Carolina coach Ray Tanner called Shaffer, a first-round pick (Tampa Bay) in June, the best hitter in college baseball last season. Shaffer's slugging percentage (.573) was more than 140 points better than the team's second-best mark, that of the graduated Phil Pohl (.435). Shaffer's team-best on-base percentage (.480) was nearly 100 points better than Brittle's .384 OBP.
Who can hit in the middle of the Clemson lineup?
"McGibbon is a kid who has some power," Leggett said, "and Shane Kennedy, an incoming JUCO kid, has a chance to help is the middle of the lineup. They are the kids with the most power."
Clemson figures to become a team even more reliant on small ball. Some believe this team is better suited to such an approach as it welcomes an influx of athletic freshmen like outfielder Maleeke Gibson and shortstop Tyler Krieger, who are competing for starting roles.
Wilkerson or Krieger could start at short.
"Top to bottom it's a great class, a lot of good pitching," Wilkerson said of the freshmen. "They are pushing everyone."
While 6-5 lefty freshman Zach Erwin and fellow rookie Clate Schmidt, who has touched 96 mph, figure to log innings early, the incoming player who has the highest upside is lefty Matthew Crownover, who has two player of the year honors in Georgia on his resume.
Perhaps only Tommy John surgery last March kept him from going straight to professional baseball as Crownover has rare velocity for a lefty, a fastball that has touched 94 mph. He was Baseball America's No. 87 prep prospect prior to his surgery last year.
"He's way ahead of where any other normal human being would be," said Leggett of Crownover's recovery.
Unlike on offense, there is an anchor to build around in starting pitcher Daniel Gossett.
The sophomore looked like a potential ace at the close of last season, when he was in line for a victory against South Carolina last June 2 in the Columbia Regional, leaving with the lead after throwing seven innings of four-hit ball, allowing two runs, three walks while striking out eight.
Gossett struck out 87 batters in 77 innings last season, finishing with a 6-3 record and 4.32 ERA. He figures to be the team's Friday starter after top arms in Kevin Brady and Dominic Leone turned pro.
"They've been real hard on me on gaining weight, eating all I can," said the 6-foot, 170-pound Gossett. "I'm working on my changeup and it's coming along well. It's good to see because it was one of my missing links last year."
Behind Gossett are experienced arms in weekend starter Kevin Pohle, relief ace Scott Firth and Matt Campbell.
But it is Gossett who must emerge as an ace, a new star for a new-look team.
"He was our No. 1 guy at the end of the year," Leggett said. "He's ready to go."


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Craftsman 42" 19.5hp* Automatic Lawn Tractor Non CA
$449.97 - $649.97
Store pickup
1 year parts and labor
Craftsman Automatic Riding Tractor Makes Lawn Care Enjoyable Do you ever wish that maintaining your yard could be as simple as driving your car – fulfill the desire with this Craftsman 19.5hp 42inch Automatic Lawn Tractor . Equipped with a foot controlled automatic mowing is as easy as stepping on the gas. Ride your lawn mower with adjustable speeds ranging from 0 to 5.5 MPH as you effortlessly tackle any type of terrain. A powerful Briggs & Stratton® 19.5 HP* OHV engine gets you going with more power, longer engine life and improved fuel economy. Get the flexibility, precision and performance you would expect from a Craftsman Automatic Riding Tractor achieving more quality yard work in least amount of time. The wide 42 inch deck covers more ground in each pass and offers adjustable cutting heights from 1-1/2 to 4 inches. The low maintenance machine is built to last thanks to a reinforced steel hood design, rugged 13-gauge channel frame and a steel front axle. Reliable Turf Saver Tires let you navigate around obstacles and landscaping boundaries with the tractor's tight 18 inch turning radius and prevents skidding and grass damage while making turns. The mower deck's gauge wheels help minimize lawn scalping. A deluxe padded mid-back seat with an automotive-inspired slide adjustment capability delivers premium comfort and makes every ride a fun, almost relaxing, experience. An easy-grip steering wheel is ergonomically designed for better control and posture and reduces fatigue. Throw in the built-in cup holder so your favorite drink securely sits by your side, keeping your lawn in top shape is a breeze.
- The Craftsman 19.5hp 42inch Automatic Lawn Tractor drives with the same comfort and ease as your car
- Ideal for larger lots, gets more lawn work done in less time
- Briggs & Stratton® 19.5 HP* OHV engine delivers more power, longer engine life and improved fuel economy, and features spin-on oil filter for extended life
- Automatic foot pedal controlled transmission makes mowing fast and easy
- 42 in. deck offers adjustable cutting heights from 1.5 to 4 in. to provide the flexibility and great cutting performance you expect from a Craftsman® tractor
- Includes gauge wheels to minimize lawn scalping and create a more beautiful lawn
- Turf Saver Tires prevent skidding and grass damage while making turns
- Reinforced steel hood design with resin side ventilators and headlight is unique to Craftsman® tractors
- Wider step-thru design eliminates obstructions beneath the steering column for easier access on and off your tractor
- Rugged 13-gauge channel frame
- Steel front axle with 10-year warranty provides strength and reduces vibration
- Steel brace locks down fuel tank for maximum stability
- Deluxe mid-back seat has added padding to make every ride a comfortable one
- Seat features auto slide adjustability that allows you to easily move the seat forward and backward
- 13 in. ergonomically designed steering wheel is easy to grip and control
- Cup holder for your favorite beverage
- Navigate around obstacles and against edges with a tight 18 in. turning radius
- Tackle different types of terrain with adjustable speeds that range from 0 to 5.5 MPH
- Accepts snow blade #24441
- 2-year full warranty backed by Sears In Home Service
- Not for sale in California
*As rated by the engine manufacturer
Specifications & Dimensions
Product Documentation
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Kerrang! Magazine 07/04/2010 - Look for an exclusive Tre Cool poster!
July 03, 2010
July 03, 2010
Green Day and Billy Talent on July 1st in Mainz, Germany.
July 02, 2010
New photos just added to our online gallery from June 26th in Paris: HERE.
July 02, 2010
Serena Williams is always closely guarded wherever she goes to avoid the threat of stalkers but now, it seems, the boot is on the other foot.
The defending champion is such a big fan of American rock band Green Day that she has lost count of the number of times she has seen them play.
"I'm a bit of a stalker," she joked. "I don't know how many I've been to, maybe too much. It's got to the point where I know the run of the show. I always know when the last song is coming, the encore. So it's a bit much. If I were them, I'd kind of watch out for me."
Serena also revealed how she would
July 02, 2010
New photos from Green Day's recent gig in Manchester: HERE.


text:
The 5 Worst Things People Do When Trying to 'Help'
#2. Declaring Their Problem Solved, Then Walking Away
Jupiterimages/Polka Dot/Getty Images
It's in our nature to want quick fixes. The best charities are the ones where you can just easily hand over a few dollars and then go about the rest of your day, knowing it's going to be used by honest, upstanding people. Don't ever think that's a bad thing. The fact that you're helping out a good cause at all is super fucking admirable, and I respect the hell out of people who do it.
But it's also easy to get tricked into that frame of mind when helping out a friend because we want their problems to be like a movie: Here's the part where they're struggling, then here's the part where they get rescued and everything is fine again. Roll credits! But in virtually every case where a person needs help, the problem cannot be boiled down to a simple one-shot cause like "addiction" or "a bad relationship." Most of those problems are caused by a deeper, darker undercurrent, something that bubbles up from time to time, manifesting itself in different ways. It's frustrating to see the same mistakes and bad habits bite them in the ass again and again. You start to feel like they're your patient instead of your friend.
Creatas Images/Creatas/Getty Images
"Mmhmm. Now tell me about your bitchass sister."
But that's how real life is different. In a movie, once a person goes through rehab, her drug problem is over. When a person starts laughing and joking, his depression is cured. In reality, people can and do suffer from this shit their entire lives. Even if we're not talking about actual illnesses (in which case, your first advice should always be "see a fucking professional instead of my dumb ass"), the destructive habits all of us have are the result of decades of repetition and reinforcement. That shit doesn't change overnight, no matter what background music you play over your homemade montage.
Hemera Technologies/AbleStock.com
"You're the BEST! Around! Nothin's gonna ever keep you d- OH MY GOD, HE'S DEAD!"
If you want to continue being their friend, then that means you still have to be there for them. That means checking in even when they're in one of those stretches where they're not fun to be around. It doesn't even have to be a big deal -- a call, an email, stopping by on your way home from work. Just knowing that someone out there gives a shit is more help than you'll ever realize (if you're lucky). I've lost count of how many messages I've gotten from readers saying that nobody cares about them or their problems. As a friend, 10 minutes of your time could easily change all of that. If you don't believe me, try it.
#1. Not Knowing When to Back Off
Jupiterimages/Comstock/Getty Images
This is the single hardest part of being a friend, and by far the hardest to know when to implement. Let me give you an example:
One of my family members was, like me, an addict. But also a career criminal to boot. He spent a massive amount of his life in prison for ... um ... "borrowing" other people's things to support his ... um ... "huge drug problem." Our family reached out to him many times, offering places to stay, jobs, money, food, and anything we could reasonably sacrifice on our end. But his cycle of uncountable crises continued for most of his life. He'd clean up and do great for a year or two, then fall back into a self-destructive pattern that would land him in prison once again. Here's the crazy part: At no point (when he was clean) did he ever consciously look around at us and say to himself, "I think I'll fuck over this person for personal gain."
Jupiterimages/Photos.com/Getty Images
"I know Grandma has some weed hidden in the bathroom. I'm ransacking that shit."
But each time that cycle started back up, all of our help was flushed down the shitter. His problems were out of his control ... but more importantly for us, they were out of our control. There came a point where we had to finally grit our teeth and say, "We've helped as much as we can, and his disasters are now affecting our own families. Helping him at this point is just perpetuating a cycle that we cannot end." Then we all stripped naked and ran through a field, screaming, "FREEDOM!"
Did it make us bad people? To some, it would seem that way. Our kids certainly didn't think so when the danger of break-ins and the volatile atmosphere disappeared.
Regardless, I cannot stress enough how dangerous this point can be -- because if you decide to pull out at the wrong time, you could be fucking your friend out of what could potentially be life-changing input. That ... didn't sound clean, did it? If you wait too long, you're letting their problems spread to you and your family like a case of emotional crabs. I understand that the Internet seems to universally hate Dr. Phil, but one of the wisest pieces of relationship advice I've ever heard came from him:
Kevin Winter/Getty Images Entertainment/Getty Images
Don't look directly at it. It's not worth losing your sanity.
"Ask yourself, 'What is it costing me to be in this relationship?' If the answer is your dreams, identity, or dignity, the cost is too high."
In that quote, his ridiculous child-molester mustache was talking about bad romantic relationships, but it applies just as easily to friendships. You can only sacrifice so much of yourself on their behalf before you finally have to step back and say, "Enough. I've done all I can. It's time for them to deal with this on their own, regardless of the consequences." Good-hearted people will have such a hard time coming to that decision. They'll feel guilt, shame, anger -- basically all the stages of grief. But in extreme cases, you have to eventually put your own sanity and health at the top of the Good Deeds queue. Otherwise, your friend could be in a dramatically worse situation in which they never change their behavior for the better, and instead grow a dependency on you to dig them out of the quickshit pit. There is no help in that. It only sustains their problems, perpetually, until one of you gives up.
Or, worse, ends up on a massive ratings factory of a reality show. People seem to be making a pretty good living out of being a fuckup these days. Whatever, you see what I'm getting at: There's a point where you're not helping, but you still want to be the hero, and in the process of trying to be the hero, you're hurting everyone else. So print this out and hang it on your wall somewhere: Sometimes being a nice person is all about knowing when to be an asshole.
John has a new book of quotes out for $0.99 right here. He also has a Twitter and a Facebook fan page. So suck it.


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As the NBA playoffs get underway, more than half the league has already moved on to the offseason. Front offices are scouting players and judging draft picks without knowing where they will be picking (if they are in the lottery) or if there will even be a season next year.
The 2011 NBA Draft picture has yet to clear. Some players have yet to decide whether they are entering the draft, the lottery balls have yet to be picked and the draft order is still in flux.
If the Minnesota Timberwolves get the No. 1 overall pick, they will be faced with a choice between two of the most talented players in the country: Derrick Williams and Kyrie Irving.
Derrick Williams had a spectacular tournament for Arizona and has a unique blend of size and skill. Minnesota will not be able to pass up the best pure scorer in the draft with pick No. 1.
1. Minn. Timberwolves: Derrick Williams
3. Toronto Raptors: Donatas Motiejunas
17. New York Knicks: Kenneth Faried
18. Washington Wizards: Klay Thompson
19. Charlotte Bobcats: Chris Singleton
20. Minn. Timberwolves: Nolan Smith
5. Sacramento Kings: Kemba Walker
21. Portland Trailblazers: Tobias Harris
9. Charlotte Bobcats: Alec Burks
25. Boston Celtics: Marshon Brooks
13. Phoenix Suns: Jordan Hamilton
29. San Antonio Spurs: Jordan Williams


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Quality Education in the Benedictine Tradition
March 15, 2013
The 24th annual Saint Vincent Summer Theatre Gala is set for 6:30 p.m. Friday, July 12 in the Robert S. Carey Student Center at Saint Vincent College, Latrobe. The gala, which celebrates the 45th season of professional theatre at Saint Vincent, will feature an evening of gourmet foods, entertainment and the theatre's production of I Love a Piano featuring the music of Irving Berlin. Jim and Maureen Laffey (standing center) of Carnegie will serve as the volunteer gala co-chairpersons. They are shown with theatre director Colleen Reilly, left, and producer/business manager Pat Reilly, right. Tickets are $125 per person. The presenting sponsors of the 2013 gala are R&L Development Company and First Commonwealth Bank. All proceeds benefit the Saint Vincent Summer Theatre. Further details are available by calling 724 805-2901.
Follow us on Twitter: @MySaintVincent Like us on Facebook:


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Sunday, November 17, 2013
The Heather Bosse State Farm Raiders were the Mount Pleasant Recreation Department
Mite Regular Season Champions and post-season Runner-up. Team members include (top row, from left) Sam Buchanan, Ziaire Coker, Matthew Parry, Michael Long, David Kenis, Dillon McCarthy, Porter Wieland, (middle row, from left) Harvey Proctor, Sawyer Thompson, Bryce Throckmorton, Alex Thompson, Blaine Throckmorton, Matt Stewart, (bottom row, from left) Alec Satter, Eddie Gubinski, Jake McQuaid, John Westmoreland, Mark Jellema. The coaches were Ed Long, Stewart Parry, Heath Thompson, Bruce Throckmorton and John Jellema. (Not pictured is Sean McCarthy.)
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A great Basel institution, and the place to go when it comes to buying typical Basel delicacies such as Läckerli, Baselbieter Rahmtäfeli and a host of other sweet temptations.
A real Basel speciality are "Läckerli" biscuits. At the Läckerli House in the Gerbergasse you will find the largest selection of this sweet delicacy. Shopping here is a treat for your eyes too.


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Steam Iron with 3-Way Auto Shut-Off
Details http://shop.panasonic.com/support-only/NI-C77XR.html
Steam Iron with 3-Way Auto Shut-Off, See-Through Detachable Water Tank, Adjustable Steam and Curved Nickel-Coated Stainless-Steel Soleplate
SpecificationsExpand All
More Details
- Built-in Retractable Cord Reel No
- Automatic Retractable Cord Reel Yes
- Detachable Water Tank Yes
- Rated Power 120V, 1200W
- Temperature Range 176°F - 392°F
- 3-Way Auto Shut-Off Yes
- Adjustable Steam Yes
- Pushbutton Steam/Dry Selector Yes
- Non-Stick Coating Yes
- Spray Mist Yes
- Jet-of-Steam/Self-Cleaning Yes
- Vertical Steam Yes
- Anti-Calcium System Yes
- Curved Soleplate Nickel Coated
- Cordless No
- 1400 Watts No
- Electric Temperature Control No
- All-Temperature Steam/Dry Cleaning No
- Heat-Resistant Carrying Case No
- Accessories No
- Dimensions (H x W x D) 4 5/16'' x 10 7/16'' x 5 5/16''
- Weight 3.0 lbs
Owner's Manuals
Owner's Manuals and Documentation
Warranty information
FAQsFrequently asked questions about your product
Will my steam/dry iron stick to fabric when spray starch is used while ironing?
No. The steam/dry iron will not stick to the fabric when ironing with starch.  Selecting the correct temperature will prevent fabric from sticking to the iron.
Where can I order parts for my product?
You can order parts and accessories for your machine from our Parts & Accessories Department at 1-800-833-9626.
What is the recommended voltage for this steam/dry iron?
The recommended voltage for this steam/dry iron is 120V.
The steam/dry iron is emitting a white powdery substance while ironing, especially when using the Jet of Steam function. How can I fix this issue?
The powder or particles are impurities and minerals found in water. These particles can be brushed off  the clothing or fabric.
Sometimes my clothes become discolored or marked after they have been ironed. How can I fix this issue?
Set the temperature correctly according to the fabric being ironed. If there is cleaning residue on the fabric, the clothes may become brown after being ironed.
Is it necessary to use distilled water in my steam/dry iron?
Using regular water may cause build up of calcium or lime in the iron's steam vents. We suggest using distilled water to prevent this build up from occurring.
I can no longer power on this steam/dry iron. How can I fix this issue?
Check to make sure that the power cord is securely inserted into the wall outlet. If it is connected properly, try another power outlet.
How can I use the spray function on this steam/dry iron?
The spray feature is useful mainly when trying to remove stubborn wrinkles and where extra dampening is needed. This function may be used for steam or dry ironing. To use the spray function simply press the "Spray Mist" button whenever spray is desired. If spray does not come out immediately, keep pressing until it comes. To get a finer spray, press the button vigorously and to get a more powerful spray, press the button more slowly.
How can I register for the warranty of my product?
You may register your products online by clicking here. It is not necessary to submit this form in order to effectuate the limited warranty. As long as there is a copy of the receipt, your product warranty begins from the date of purchase.
Does this steam/dry iron have a retractable power cord?
Yes. The power cord is retractable.
Can I use this steam/dry iron to vertically steam my garment?
Yes. Hang the garment on a clothes hanger to get the best results when vertically steaming. Curtains and drapes may also be steamed once they are hung. Hold the iron close to the fabric then hold the fabric tightly with your free hand and pump the Vertical Steam button (Jet of Steam button) at 5 second intervals as you pass the iron over the wrinkled fabric.


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Japanese Oribe ware bowl has characteristic green copper glaze and bold, hand-drawn design. Fill it with rice, sand, or ash to hold incense sticks. We suggest pairing the Zen Circle Incense bowl with the Go-zan Incense Sampler (#4324S) for a gift that will be most appreciated and ready to enjoy.
4" dia., 3" h
Order any time: 800.794.9862 . FAX 978.620.1352 . www.dharmacrafts.com Contact Customer Service©2000 - 2016 DharmaCrafts, Inc.


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GIDEON Wallet - null
Item# AC7533
Detailshttp://www.kipling-usa.com/gideon-wallet/AC7533.html GIDEON Wallet
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Keep your daily documents safe and in style with this great wallet, crafted with multiple interior compartments and a convenient strap to transform this essential in to a wristlet! Perfect for the workday or girl's night out. Dimensions: 3.75" x 6.75" x 1"Read More
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Myeloma patients who undergo stem cell/bone marrow transplantation may experience a decrease in antibody titers to vaccine-preventable diseases such as tetanus, polio, and encapsulated organisms during the first 4 years after transplantation. Therefore, revaccination of transplant recipients is routinely recommended.
At the present time, vaccine recommendations for recipients of allogeneic and autologous transplants are the same (see CDC guidelines and European Group for Blood and Marrow Transplantation guidelines). Current guidelines suggest tetanus-dipheria, H. Influenza, and hepatitis B vaccine at 12, 14, and 24 months after stem cell transplant. The hepatitis B vaccine may be omitted in adult patients with no risk factors for this virus. Influenza vaccination should be given before the transplant and then annually starting 6 months after the transplant. Pneumococcal vaccine should be given 12 and 24 months after transplant. Measles, mumps, and rubella is a live vaccine and should be avoided for at least 24 months and should only be given if the patient is thought to be immunocompetent.
Physicians caring for patients with multiple myeloma can get further information about vaccine recommendations by calling the National Immunization Hotline at (800) 232-2522 or by accessing the CDC website at www.cdc.gov/nip.
Note: Myeloma patients must keep in mind two concepts when reaching a decision about vaccination.


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Call our customer service representatives and get expert advices for your Dremel tool.
Contact our customer service representatives and get expert advice for your Dremel tool
The Dremel CKDR-02 three-tool kit includes everything you will need to repair, remodel, restore, cut, sand, and more. Between the Dremel 3000 variable-speed rotary tool, the Multi-Max MM20 oscillating tool, and the Saw-Max cutting tool, you will be able to multi-task your way through a variety of projects at home, in the shop, or jobsite. A soft storage bag and 15 accessories are included. This three-tool combo kit offers ultimate value and versatility. Included tools have a two year warranty.


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Welcome to Hyperion Records, an independent British classical label devoted to presenting high-quality recordings of music of all styles and from all periods from the twelfth century to the twenty-first.
Hyperion offers both CDs, and downloads in a number of formats. The site is also available in several languages.
Please use the dropdown buttons to set your preferred options, or use the checkbox to accept the defaults.
|Haydn: Tost I Quartets Op 54
'Well-nigh perfect throughout' (Gramophone)
'This is one of the very best records from these excellent players and the recording is absolutely first-class' (The Penguin Guide to Compact Discs)» More


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Wide receiver Juwan Johnson has incredible upside.
The same could be said for the 2015 Penn State football recruiting class, even more so now because Johnson is a part of it.
Johnson, a 6-foot-5, 205-pound pass-catcher from Glassboro, N.J., became the latest prospect to verbally commit to new coach James Franklin's staff on Sunday. The eighth member of the Lions' 2015 recruiting class, Johnson was in University Park this weekend on an unofficial visit.
At least five of those players are consensus four-star prospects, putting Penn State's early class at No. 5 in the country according to 247Sports ranking index.
Johnson is the highest-rated player yet to join up with the Lions, checking in as 247Sport's 55th-best prospect in the country. Johnson picked Penn State over Ohio State, Michigan State, Michigan and Boston College, but he received an offer a week ago from Alabama.
Not only is Johnson a big receiver, he is fast. He's been timed at 4.45 seconds in the 40-yard dash and has clocked at 10.6 in the 100 meters. He's already been picked for next January's U.S. Army All-American Bowl.
"Offensively, his size and his speed are his biggest strengths. He's 6-5 and can run like a deer," Glassboro coach Mark Maccarone told NJ.com recently. "Defensively, he is a great lock-down corner for us. The truth is, he's an even better defensive player than he is an offensive player."
Johnson was third-team all-Group 1 New Jersey despite being an ankle injury that limited him to 26 receptions for 421 yards and four touchdowns as a junior. His brother, George Johnson, played defensive end at Rutgers.
Penn State probably will look to add one or two more receivers in this class. Tennessee's Van Jefferson is near the top of the list, and the Lions also have been involved with Missouri's Alex Ofodile and New Jersey's Irvin Charles - Jefferson and Ofodile are consensus four-star prospects, while Charles has a four-star rating from 247Sports.
- Philip Cmor


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Top Definition
When Somebody hits up mcdonalds and spends all their $ on the dollar menu because they are too poor to afford the big mac. Common items are mcdouble, chicken sandwich, 4 pc nuggets and small soda. (usually about 5-7 dollars)
Hey Tayshawn, Wanna go dolla menu broke for lunch? I'm hungry as shit
de tbiggy 05 Martie 2010
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Can you define these popular missing words?


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Community Information Database
You are browsing the Subject Heading Young Adults which contains 7 record(s)
- Alternative Education Programs Santa Cruz County Office of Education
Our programs are student-centered and adapt to meet individual needs. Staff works collaboratively to facilitate learning and change. Dedicated teachers assist students to achieve social, interpersonal... [Full Record] [Add to Personal List]
- Girls For A Change
Girls For A Change (GFC) is a national organization that empowers thousands of teen girls to create and lead social change. GFC provides girls with professional female role models, leadership training... [Full Record] [Add to Personal List]
- Healthy Kids of Santa Cruz County
Healthy Kids of Santa Cruz County offers medical, dental and vision insurance to children living in Santa Cruz County, that do not qualify for existing state and county health insurance programs. E... [Full Record] [Add to Personal List]
- Mountain Community Theater
Mountain Community Theater is Santa Cruz Countys longest continuously running community theater company. MCT is a member-run non-profit organization that derives its support primarily from performanc... [Full Record] [Add to Personal List]
- Theatre In The Mountains
Theatre in the Mountains is dedicated to providing theatrical opportunities for children and adults throughout the year. The performances are held in the Loma Prieta Community Foundation/CT English Mi... [Full Record] [Add to Personal List]
- Toadal Fitness -4- Kids
Live Oak Location: 1200 17th Ave., Santa Cruz, CA 95062 831-464-3764 Westside location: 2929 Mission Street. Santa Cruz, Ca 95060 831-466-3764 Scotts Valley location: 269 Mount Hermon Rd. ... [Full Record] [Add to Personal List]
- Youth Serve
Youth Serve, a program of the Volunteer Center of Santa Cruz County, is a program that works with youth 11-18 finding them community service opportunities all around he county. We engage youth on a we... [Full Record]


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JERRY McBRIDE/Durango Herald
JERRY McBRIDE/Durango Herald
Leslie Knopes, the politically ambitious city employee portrayed by Amy Poehler on the sitcom, �Parks and Recreation,� has nothing on Durango�s real-life parks and recreation director.
Cathy Metz recently was named president of the Colorado Parks and Recreation Association after serving two years as its president-elect and vice president.
�It�s a great honor,� Metz said. She now will �have a larger role advocating for parks and recreation on a statewide basis.�
The professional organization has about 1,000 members and represents the interests of more than 300 cities, counties and recreational districts.
Despite its name, the association is not all about fun and games. The group has a lobbyist to monitor legislation and look after its political interests in Denver.
In these tight economic times, �everybody is looking for opportunities of funding,� Metz said.
So the parks and recreation association tries to prevent raiding of the state�s lottery fund, which is the source of the Great Outdoors Colorado grants used by local governments to acquire public lands and build parks.
In another honor received by the city at a recent conference in Keystone, Durango was recognized with the Columbine Award in Innovation for Extraordinary Partnerships.
The city works with Fort Lewis College and local groups such as Trails 2000 and the Durango Winter Sports Foundation to do projects that the city otherwise could not afford.
Metz, the city�s parks and recreation director for the last 17 years, cited the multiuse playing fields at the college, improvements to Chapman Hill and soft trails on public lands as just a few examples of partnership projects.
�There�s over 100 miles of trail around Durango,� Metz said. �That�s really unusual to have that many trails around a town this size. So when people talk about Durango, they talk about it being a trail mecca. We wouldn�t have them without the partnership and assistance of Trails 2000.�


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12 News Contributor Kidd O'Shea spoke with A-List winner for "Best Personal Injury Law" on the red carpet of the awards ceremony.
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SMU suffers 60-52 loss against Memphis
Photo: Cooper Neill/Staff Photographer
Cannen Cunningham #15 of the SMU Mustangs blocks the shot of Adonis Thomas #4 of the Memphis Tigers at Moody Coliseum on Wednesday, February 6, 2013 in University Park, Texas.
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0 Members and 1 Guest are viewing this topic.
... other canes, I had described as non-descript, appear to have more similarities to the older canes described before the 67 period canes.
So, is this a 67 Starthearn composed of Ysart "leftovers"?
How would this paperweight be described?
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Mayor's Marathon deadlines approaching
Related Links
The 39th annual marathon is scheduled for June 23.
Registration is available online at www.mayorsmarathon.com.
The Mayor's Marathon Expo at the Sheraton, which will include race packet pickup, is scheduled for 3 p.m. to 8 p.m. on June 21 and 10 a.m. to 6 p.m. on June 22. No race-day registration or race packet pickups will be available.
The marathon (26.2 miles) and half-marathon (13.1 miles) events are certified by USA Track and Field. In addition to the marathon and half-marathon, three other individual events are offered - a 26.2-mile marathon walk, 5-miler and 1.6-mile Youth Cup. Also, back is the popular marathon relay, featuring four-member teams running 6.5-mile legs.


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Todd Outman has not encountered many problems since becoming Wellsboro's boys basketball coach three seasons ago. He has led the Green Hornets to two straight NTL West championships and 41 wins.
This preseason, though, Outman finally found a problem. The third-year coach said his team was being too unselfish. As problems go on a scale of 1-10, it's probably rates a one. In fact, it's not a bad problem to have.
Wellsboro put its unselfishness to good use at the South Williamsport Tournament last week, defeating Muncy and South by 37 combined points while capturing the inaugural championship. The Hornets have four starters back from last year's 20-4 team and showed that they are as cohesive as they are talented with two wins over quality teams.
"The kids' court vision is really good and we've been working on that a lot because we're having some problems where we're penetrating too far at times, but (this weekend) they seemed to be penetrating at the right time and that was really causing everyone to shift over. The kids are quick and a little above average ball-handlers and they can cause some problems."
Wellsboro averaged 69 points in its wins and its balance is a big strength. Senior point guard Jordan Jackson is one of the area's best but has plenty of help. Dylan Prough, Matt Reese, Joey Doganiero, Michael Pietropola and Kieron Smethers also played well and are capable of big games every night out. Doganiero scored a game-high 19 points against Muncy while Prough had a double-double and Smethers added five rebounds and five assists. All five starters reached double figures in at least one game.
The Hornets executed well in the second half of both wins and blew open what had been a close game against South, outscoring it 38-21 in the second half. It was an impressive performance against a team that eliminated it in last year's District 4 Class AA quarterfinals. It also could point toward good things to come.
Because the team is so balanced and gets along so well that has led to being too unselfish in the past. Instead of taking the easy shot, the Hornets have made one too many passes. But when just the right amount of passing is going on, Wellsboro is tough to stop. And fun to watch.
"That is a great team," Muncy coach Jason Gresh said. "That is probably one of the best teams we will see all year."
And what started out as a potential problem could become a huge strength.
GOOD REVIEWS: South and Muncy had plenty of questions entering the opening weekend. South had to replace nearly every contributor from last year while Muncy is playing without its top two scorers from last year. While it is still ridiculously early to tell where their seasons are going, both South and Muncy showed plenty of promise.
The Mounties had just one player, Riley Hengler, who was part of last year's rotation playing last weekend. Still, the Mounties looked good in a 75-57 win over Canton before hanging with Wellsboro throughout the first half. South might experience some growing pains as so many players are getting their first varsity experience but there is a lot of potential there, not just for this season but the next few as well.
"There's potential for this year and, looking ahead, a lot of these guys will get to play together for hopefully 50 games or so," South coach Matt Fisher said. "We had three more sophomores on the floor that haven't played before (Friday) and they did well and were communicating which was good to see. We're trying to get guys comfortable with playing at this level and they did well."
Muncy played mostly iron-man basketball last year so new starters Nick Ring and Teddy Clark received their first significant varsity action as did reserve Jeff Scott. All played well too. Ring looks like he could be a force inside and recorded consecutive double-doubles in his first two starts while Scott and Clark showed some nice versatility.
Early Friday, it looked like Wellsboro would rout Muncy but the Indians kept fighting back and trailed by five points or fewer for much of the game. Returning starters Ammar Sabanovic, Billy Kolletzki and Dawson Fox all performed well and a team that reached states last year showed no fear as well as a relentless attitude that could serve it well all season. Sabanovic is one of the area's premier point guards and had 12 assists in Saturday's 65-44 win over Canton.
"They did a nice job and there are things we can work on and that's the key," Gresh said. "Nick played really well. Jeff Scott gave me great minutes off the bench and I couldn't ask for more from him. Getting this experience and getting their eyes used to it and getting them used to the season is huge."
PUTBACKS: St. John Neumann played well despite missing some key players in Saturday's 63-45 win at North Penn. Alize Johnson is playing under the basket this year, but has plenty of guard qualities and scored 21 points, while grabbing eight rebounds, dealing eight assists and blocking two shots ... Cowanesque Valley could be a team to watch this season. There are some promising new players, along with some quality veterans and that mix helped the Indians capture their tournament championship as they defeated Northern Potter and Bucktail. Three players averaged in double figures with Josh Nudd scoring a career-high 20 points in a 64-54 final win over the Bucks ... Hughesville held off West Perry, 52-48 to take third place at the Mifflinburg Tournament. The Spartans avoided a second straight 0-2 start which could be good for their collective psyche. Fred Newhart has a nice all-around game and scored 16 points in the win.
Dr. Masse's area top five
1. Williamsport (2-0): The Millionaires made a nice early statement, capturing the William Penn York Tournament for the first time in seven years while beating annual Class AAAA heavyweights Reading and William Penn York. Tournament MVP Jahad Thomas averaged 24 points in the wins while Isaiah Washington and Chris Kinley also had some big games. Herb Stelly and Achilles Watson are two players who could make a big difference this year and both made impacts in the wins. Watson has a lot of upside and scored 10 points against York in the final. Williamsport has three home games this week against Crestwood, Wilkes-Barre GAR and Westinghouse. If he stands are not packed I would be surprised because this is a fun team to watch.
2. Loyalsock (1-0): The Lancers pulled away from Jersey Shore and earned a convincing 74-51 in their season opener. The defending District 4 Class AA champions displayed nice balance and coach Ron Insinger has to be impressed with the performances of Ben Sosa and Mike Pastore. Sosa scored a career-high 17 points in his first start at forward while Pastore added 11 points off the bench. Omar Little scored a career-high 19 points while Roger Wilson and Kyle Datres had double-doubles. Loyalsock is off until Friday and faces a good test at home against Class AAAA Central Mountain.
3. Lewisburg (0-0): The Green Dragons are not easing into their schedule, going on the road Tuesday and Thursday to face Shikellamy and South Williamsport. The Braves reached last year's District 4 Class AAA final and South finished fourth in Class AA.
4. Wellsboro (2-0): While the Hornets are an experienced team they still are somewhat young. Jackson and Reese are the only seniors in the rotation so this program could continue flourishing the next few years.
"We've been in some tight situations before. We know how to handle those situations and get out," Prough said. "We have a lot of strengths. We have a lot of weaknesses too, but those are things we can fix."
5. Sullivan County (2-0): The defending District 4 Class A champions are off to a good start after capturing their second straight Towanda Tournament championship. Like last year, the Griffins built their success around stifling defense and held both Towanda and Mansfield below 40 points. Conner Wylie often was huge off the bench last year and the forward could have a big season. Wylie was Sullivan's leading scorer last weekend and also grabbed a team-high 17 rebounds. Point guard Kelby Mullen also played well, averaging 12 points and seven rebounds.
Players of the Week
Jordan Jackson, Wellsboro and Jahad Thomas, Williamsport: I took the easy way out here since I could not make up my mind. Jackson is a terrific point guard and earned South Tournament MVP honors after scoring 35 points. He had nine rebounds and seven assists against Muncy. Thomas was outstanding in both Williamsport wins and really can do it all on the court. Division I colleges might think Thomas is just a power forward but he really can play any position and is one of the state's most well-rounded players.
Game of the Week
South Williamsport at Hughesville: A good local rivalry is renewed Tuesday and this should be an interesting matchup between teams that have the potential to be major district playoff players. Hughesville won a memorable district semifinal over South the last time they played in 2010 after South made a huge comeback. It also is a Jersey Shore reunion as former Bulldog standouts Matt Fisher and Nick Tagliaferri coach South and Hughesville.


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Top Definition
Common slang used in the adult entertainment industry and on the streets. It means to give oral sex without a condom. Adds for escorts will commonly state that they, "allow full french" as part of the experience.
She's an escort that provide full girlfriend experience, however doesn't do full french.
door Mike from NH 10 maart 2006
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Monday, September 30, 2013
On Saturday, Oct. 5, Mount Pleasant will once again welcome marching bands from around the Lowcountry and the southeast for the annual Lowcountry Invitational (LCI) Marching Festival to be held at the Wando High School Stadium on Mathis Ferry Road. The Bands of Wando Boosters sponsors this annual event that provides a national-level music education and adjudication experience for more than 1,500 musicians and visual artists. These young performance artists will be judged by some of the best music and visual educators in North America, and spectators will be entertained by music, pageantry and choreography as 14 bands are scheduled to compete.
Of course, concessions will be plentiful including the standard fare of hotdogs and hamburgers, but also featuring food from local restaurants offered at reasonable prices for the entire family to enjoy. Other areas of interest include "Vicki's Village," which will offer marching-band themed items such as jewelry and T-shirts. Another guaranteed popular area will be "The Fastest Drummer" competition. Plenty of directed parking will be available and assistance as well for those who need it.
Following the competition, eight-time 5A State Marching Band Champions, the Wando High School Marching Band, will perform in exhibition mode this year's show entitled, "It's All WRIGHT.""
The 2013 LCI coordinators are Donovan and Vickie Powell who are also the president-elect(s) for the Bands of Wando Boosters. Scott Rush is the Director of the Bands of Wando.
If you go
WHAT: Lowcountry Invitational Marching Festival
WHEN: Saturday, Oct. 5; Gates Open at 1:30 p.m. Competition begins at 3 p.m.; Awards at 8:45 p.m.
WHERE: Wando High School Stadium, Mathis Ferry Road, Mount Pleasant
ADMISSION: Adults $8 and includes program; Students $5; children under 4 are free. Cario, Laing and Moultrie band students who wear their band shirts admitted free.
FOR MORE INFORMATION: Visit www.wandobands.org. or call Rebecca Imholz, LCI publicity at 693-0537.
Class A bands
Berkeley: 3 p.m.
Bamberg-Earhardt: 3:15 p.m.
Class AA bands
Pendleton: 3:45 p.m.
Waccamaw: 4 p.m.
Class AAA bands
Hanahan: 4:30 p.m.
James Island: 4:45 p.m.
Cane Bay: 5 p.m.
Class AAAA bands
West Ashley: 5:30 p.m.
Colleton County: 5:45 p.m.
Fort Dorchester: 6 p.m.
Blythewood: 6:15 p.m.
Class AAAAA bands
Ashley Ridge: 7:30 p.m.
Summerville: 7:45 p.m.
Fort Mill: 8 p.m.
Wando exhibition: 8:15 p.m.
Awards: 8:45 p.m.
© 2016 Moultrie News an Evening Post Industries company. All Rights Reserved.
Registration on or use of this site constitutes acceptance of our Terms of Service, Privacy Policy and Parental Consent Form.


text:
Source: Microsoft
quote: It takes place three years after "Halo 3" ended, and represents the return of the Master Chief as the protagonist.
quote: "Halo 4's" plot is centralized around "Master Chief" and his ability to kill evil aliens while trying to save his AI friend, Cortana.
quote: The bundle, which appears to include the special edition Halo 4 console, the Halo 4 game, and two controllers, was rumored to run around $399.99 while a stand-alone controller will cost $49.99.


text:
It's a bird, it's a plane, it's ... Google!
Many Nevada County residents were puzzled by multiple objects in the sky Friday, speculating on comets and drones. In fact, it appears they are part of a new Google Internet project.
Google confirmed its balloons could be in the area as part of Project Loon, a worldwide Internet movement involving the launch of white transparent balloons with Internet receptors into the stratosphere.
These balloons float twice as high as airplanes and are carried around the Earth by winds, according to google.com/loon. Users connect to the balloon network using a special Internet antenna attached to their building.
The signal bounces from balloon to balloon, then to the global Internet back on Earth.
The balloons were launched in the California Central Valley in August, according to arstechnica.com. The tests began in August and run through the end of the year.
"They can occasionally move into that area," said Google representative Kaitlin Jabbari, regarding the sightings of balloons throughout Nevada County.
Locals reported the silvery objects to be oval-shaped, very bright and somewhat translucent.
The objects seemed to have moved farther away and higher up since the initial viewing Friday, some said.
"I have a neighbor who said that it's definitely not a drone and not a weather balloon because it would have a different shape," said Nevada City resident Harriet Totten, who first witnessed the object shortly after 12:36 p.m. Friday.
Self-proclaimed "comet hunter" Don Machholz from Colfax, who said he worked in the military, speculated that the object could have been a weather balloon, which he says are translucent.
"My guess is that it was some sort of balloon, but I was surprised that it looked so bright," he said.
"When I looked again, 20 minutes later, it was gone."
To contact Staff Writer Jennifer Terman, email firstname.lastname@example.com or call 530-477-4230.


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on February 12, 2013 - 12:23 AM
The Town of Aurora has to deal with plenty of stray dogs and problem pooches.
That's why Highway Superintendent David Gunner said it's time to cancel a longstanding contract with the Town of Wales to handle dog catching there as well.
"Dog catching is a dirty job that all towns have to do," said Gunner, who oversees Aurora's dog control operations under a recent arrangement to streamline the services from full-time to part-time under the umbrella of the town Highway Department. "It's just time to move on."
The Town Board expects to take up the issue at its work session Feb. 20.
Wales Supervisor Rickey Venditti said he was unaware of any issue with the dog control arrangement until Aurora Supervisor Jolene Jeffe contacted him last week. Gunner said that after reviewing the contract, he sent a memo Jan. 30 to the Aurora Town Board about his proposal to end the arrangement.
"I don't know what his motives are," Venditti said of Gunner. "It's been a very good and fair contract between the two towns. It's very low-key and is not that demanding."
Wales pays $2,900 a year to Aurora for dog control coverage. A 90-day cancellation clause is in place for either town to end the contract early, and that's what Gunner wants to do. The contract is set to run through the end of the year.
Wales averages about three dog control calls a month, Gunner said, but a trying incident Jan. 11 on Schang Road in Wales helped cement his desire to end Aurora's dog-catching days in Wales.
Gunner said a Wales resident shot and killed another neighbor's family dog with a 12-gauge shotgun, apparently because the dog killed one of his chickens.
"The sheriff called us in. It was pretty gruesome and was a very emotional scene," Gunner said. "For me, it was the turning point. Why are we doing this? It's just not a good thing."
Venditti said he is disappointed in Gunner's stance.
"The sad thing is this is what governments are trying to do – some mutual consolidation of services," Venditti said. "For the low quantity of calls, it was a very fair agreement."
The Aurora Town Board in December appointed four assistant dog control officers, who are part-time parks workers, to be "on call" and handle dog calls on a rotating basis. That move was part of the plan to move away from a full-time dog control operation in Aurora.
email: firstname.lastname@example.com


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BG: My DH is deployed, I live 2000 miles away from friends and family, I have a dd 8 (about to be 9) and ds 2.
I just posted this note to my daughter:
-EVERY SINGLE LITTLE THING IS PICKED UP OFF OF THE LIVING ROOM/PARLOR ROOM/DINING ROOM FLOOR. I am tired of the dog eating everything. I came downstairs after putting DS down to find the frog potty and a tooth brush destroyed. I will help you clean everything up but YOU have to help maintain it.
-I am done repeating myself. The cat needs to be fed every single day, so do the dogs. I will be making you a chore chart if that will help
-Pick up after yourself. Do not leave shoes/clothes/jackets, etc laying around. They will be eaten by the dog.
-The dogs need to go out every two hours. Yes I know they are a pain. We have a chain outside. Latch dog 1 to it (I will get her collar out of the car) and let dog2 out.(BG: Dog 2 refuses to poop or pee on a leash, collar chain etc) Dog2 will not wander far if dog1 isnâ€ââ€Â¢t with him. (I have a big backyard and they like to hang out in the far corners)
She is almost 9 and typically she is wonderful. Great at school, listens well, etc. I know it's been tough since her dad deployed (a few days before christmas and wont be back for at least 6 more months) but I just can not let her skate anymore. After I rocked DS (since his dad has deployed he wont sleep with the lights off and screams unless he's rocked to sleep, he's become super clingy and barely lets me leave his sight) I came downstairs to find shit and pee all over the floor, the frog potty chewed and toothbrush chewed. I was furious so I wrote the note. I then found she didnt do the 2 other things I'd asked of her (feed the cat, bring your clothes upstairs) so I woke her up and had a very very long talk. (She had just gone to bed 5 minutes before) I told her things had to change. I spent hours cleaning today and you cant even tell now. I told her I wasnt mad at her but this is unacceptable. She's getting older, she can help more. I tried to be calm and I told her I didnt want to have so many bursts of anger, i want to be the fun mom but I cant be fun until she actually listens and helps me more. She cried but idk we needed this talk. I told her that yes, she did help me pick up a few times today but that she was sassy and rude and how did she think it made me feel? She said sad and I said exactly. We cant keep going with her attitude. I need her to do what she is told to do without giving me rudeness at every turn.I told her that yes, she did help me pick up a few times today but that she was sassy and rude and how did she think it made me feel? She said sad and I said exactly. We cant keep going with her attitude. I need her to do what she is told to do without giving me rudeness at every turn. Earlier I was cleaning the upstairs so I told her to play with her brother, whom was playing with damn dolls, and she acted like I asked her to dig through trash. I cant keep letting her skate. I'm going to have a break down at this rate. I explained that she needs to put herself in my shoes and how would she feel if I was rude to her every time she needed a ride or to eat or anything else? Some things just have to be done. I tried explaining that I'm under a lot of pressure right now (I listed off responsibilities) and she needs to at the very least do what she is told to do.
I am doing the best I can, I just need some advice. I hope this made some coherent sense. I am just so stressed out, I barely sleep and I've lost 8 lbs since DH left (I didnt need/want to I'm just not eating)
I realize my post is wonky so this is for the hippos.
"suddenly start this strict enforcement of picking up after herself"
It's actually been a rule all along that was enforced more when her dad was here. I've been letting her skate recently and it seems like she's just been taking advantage of it to the point where she isnt listening anymore.
"The note puts it over the top."
The note was originally meant as an outline for a talk I was going to have with her in the morning (I have a bad memory and wanted to make sure I addressed everything) but when I saw that she didnt do the 2 things I'd reminded her of at least 3 times, she saw the note.
I'm trying very hard not to take anything out on her. I tried explaining that at the very very least she needs to do what I ask her to do. She's had chores/rules since she was 4 or 5 so it's nothing new. I'm not asking for her to do anything out of the ordinary but she needs to actually do it instead of putting it off and forgetting about it.
:Is getting rid of the dog an option?:
No, the dog is improving. I let the dogs out during the day and late at night. She only has to let them out at 4 and at 6. That's it, those are the only times.
"Why is the potty seat her job? When does she get to be a kid, between watching her brother, helping clean up, letting dogs out every 2 hrs, etc?"
For some reason she took it out of hte bathroom. Still not sure. DS isnt potty trained, it's more for show at this point. She gets to be a kid a lot actually. She has lots of privileges, she does swim classes, drama, etc. She doesnt clean up after her brother, I want her to clean up after herself. I want her to pick up her own shoes, toys etc.
So what exactly is an 8/9 year old supose to be responsible for?
All I want for her to do, and what I explained to her, is to do a few chores such as picking up after herself, letting the dogs out twice and making sure the cat is fed at night. I'm not asking her to mop, do laundry, clean the bathroom etc. but if she leave a dirty plate somewhere, put it in the sink and things like that.
"Am I understanding that she was suppose to clean her brothers poo and pee filled potty? "
Oh sweet jesus NO! The potty has never even been used. It was just in the bathroom for show because I want DS to get use to it. Once he's at that stage, she wont be responsible for it either. She was the last one in the bathroom. She was suppose to shut the door behind her. idk what happened but the potty made it into the living room chewed up.
"That is a LOT of responsibility for such a young child. Why does the responsibility of three animals fall totally on her? Why is she responsible for cleaning up after brother?"
Ok I feel like I'm just not explaining this correctly here. The animals do not fall totally on her nor does cleaning up after her brother.
I clean the litter box and feed/water the cat. She makes sure the cat has food at night. (if the cat doesnt, thefood is right by the bowl)
I take the dogs out the majority of the day (walks etc) She lets them out twice, at 4 pm and 6 pm. I feed and water the dogs. She makes sure the dogs have food and water at night. (she either says they are out or she puts food in, the food is not far from the bowls either)
I obviously clean up after ds. Today was a substantial mess and They were in the play room and then coming in my room. I was trying to sort through some laundry so I asked her to play with her brother (which they were already doing) but suddenly it was a huge deal.
We are actually having a girls night on Wednesday. I let her pick the restaurant so we are going to the melting pot, haha. She also gets an allowance so I do try to reward her. Plus this current episode is because she is the one that wants friends to come over tomorrow and I told her that she would have to help me pick up first. She has known this since 10 am this morning. Otherwise I would not be freaking out about the cleanliness of the house
" I think having regular chores is good, listing out your adult responsibilities and telling your kid she made you sad is not. It's a classic guilt trip. You need to figure out how to not take misbehavior personally."
I agree that I could have handled it better. My mom was the master of jewish guilt trips and I swore I wouldnt do them and now look at me! I wasnt aiming at a guilt trip, I was trying to teach her empathy. Poorly executed on my part
"but not putting it on her to take them out so often and keeping everything including her brother's stuff cleaned up at all times. "
When he passes the puppy stage it wont be so often. She doesnt need to keep everything off the floor at all time, just until tomorrow because she wants to have friends over. I do want her valuable things off the floor (boots, etc) because the dog is a chewer. I buy about $50 a week in chew toys for him currently so we are really trying with him
"Nobody is accusing you of being on your ass. Please don't be a martyr here. You asked for help and you simply asked if you were being unreasonable."
oh lawd here we go. No I never said nor implied that I'm a martyr. It is how you worded it. Saying that I need to take up slack implies I'm not doing enough or I am somehow lazing about. That is not the case. Yes my expectations are high but they have always been that way and she typically meets them. I think I've been giving her too much slack and therefore she's taken advantage of the situation to the point to where she isnt listening.


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I know this is the Red Wing forum, but Im hoping a toronto fan with a similar dilemma as me will come on here and see this.
I have 2 real tickets to the WC, but they are in the Toronto section. If you are a leafs fan with tickets in the Red Wings section and would like to switch, we can meet inside the stadium after we got through security and trade seats. Meeting inside allows us neither to get scammed with bad tickets.
You can DM me on this site, or email me at firstname.lastname@example.com.
My seats are section 27, row 77 I believe.


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Top Definition
An amazing young lady, she is one that is very well liked by all people. she usually enjoys children and has little patience for people who act like children. She is quite smart, and very sudious. a true friend and companion.
Wow Joe, she could be a real Laura Ann!
de The one who cares. 11 septembre 2013
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Associated Press
Associated Press
NEW YORK � Helen Gurley Brown, longtime editor of Cosmopolitan magazine who invited millions of women to join the sexual revolution, has died. She was 90.
Brown died Monday at a hospital in New York after a brief hospitalization, Hearst CEO Frank A. Bennack, Jr. said in a statement.
�Sex and the Single Girl,� her grab-bag book of advice, opinion and anecdote on why being single shouldn�t mean being sexless, made a celebrity of the 40-year-old advertising copywriter in 1962.
Three years later, she was hired by Hearst Magazines to turn around the languishing Cosmopolitan and it became her bully pulpit for the next 32 years.
She said at the outset that her aim was to tell a reader �how to get everything out of life � the money, recognition, success, men, prestige, authority, dignity � whatever she is looking at through the glass her nose is pressed against.�
�It was a terrific magazine,� she said, looking back when she surrendered the editorship of the U.S. edition in 1997. �I would want my legacy to be, �She created something that helped people.� My reader, I always felt, was someone who needed to come into her own.�
Along the way she added to the language such terms as �Cosmo girl� � hip, sexy, vivacious and smart � and �mouseburger,� which she coined first in describing herself as a plain and ordinary woman who must work relentlessly to make herself desirable and successful.
She put big-haired, deep-cleavaged beauties photographed by Francesco Scavullo on the magazine�s cover, behind teaser titles such as �Nothing Fails Like Sex-cess � Facts About Our Real Lovemaking Needs.�
Male centerfolds arrived during the 1970s � actor Burt Reynolds� (modestly) nude pose in 1972 created a sensation � but departed by the �90s.
Brown and Cosmo were anathema to militant feminists, who staged a sit-in at her office. One of them, Kate Millet, said, �The magazine�s reactionary politics were too much to take, especially the man-hunting part. The entire message seemed to be �Seduce your boss, then marry him.��
Another early critic was Betty Friedan, who dismissed the magazine as �immature teenage-level sexual fantasy,� but later came around and said Brown, �in her editorship, has been a rather spirited and gutsy example in the revolution of women.�
�Bad Girls Go Everywhere,� the 2009 biography of Brown by Jennifer Scanlon, a women�s studies professor, argued that her message of empowerment made Brown a feminist even if the movement didn�t recognize her as such.
There was no disputing that Brown quickly turned a financial turkey into a peacock.
Within four issues, circulation, which had fallen below the 800,000 readers guaranteed to advertisers, was on the rise, even with the newsstand price increasing from 35 cents to 50 and then 60.
Sales grew every year until peaking at just over 3 million in 1983, then slowly leveled off to 2.5 million at $2.95 a copy, where it was when Brown left in 1997. (She stayed on as editor in chief of the magazine�s foreign editions.)
She was still rail-thin, 5-feet-4 and within a few pounds of 100 in either direction, as she had kept herself throughout her life with daily exercise and a careful diet.
�You can�t be sexual at 60 if you�re fat,� she observed on her 60th birthday. She also championed cosmetic surgery, speaking easily of her own nose job, facelifts and silicone injections.
An ugly duckling by her own account, Helen Gurley was a child of the Ozarks, born Feb. 18, 1922 in Green Forest, Ark. Growing up in the Depression, she earned pocket money by giving other kids dance lessons.
Her father died when she was 10 and her mother, a teacher, moved the family to Los Angeles, where young Helen, acne-ridden and otherwise physically unendowed, graduated as valedictorian of John H. Francis Polytechnic High School in 1939.
All the immediate future held was secretarial work. With typing and shorthand learned at a business college, she went through 18 jobs in seven years at places such as the William Morris Agency, the Daily News in Los Angeles, and, in 1948, the Foote, Cone & Belding advertising agency. There, when finally given a shot at writing ad copy, she began winning prizes and was hired away by Kenyon & Eckhardt, which made her the highest paid advertising woman on the West Coast.
She also evidently was piling up the experience she put to use later as an author, editor and hostess of a TV chit-chat show.
�I�ve never worked anywhere without being sexually involved with somebody in the office,� she told New York magazine in 1982. Asked whether that included the boss, she said, �Why discriminate against him?�
Marriage came when she was 37 to twice-divorced David Brown, a former Cosmopolitan managing editor turned movie producer, whose credits would include �The Sting� and �Jaws.�
Her husband encouraged Brown to write a book, which she wrote on weekends, and suggested the title, �Sex and the Single Girl.�
They moved to New York after the book became one of the top sellers of 1962. Moviemakers bought it for a then-very-hefty $200,000, not for the nonexistent plot, but for its provocative title. Natalie Wood played a character named Helen Gurley Brown who had no resemblance to the original.
She followed up her success with a long-playing record album, �Lessons in Love,� and another book, �Sex in the Office,� in 1965.
That year she and her husband pitched a women�s magazine idea at Hearst, which turned it down, but hired her to run Cosmopolitan instead.
In 1967, she hosted a TV talk show, �Outrageous Opinions,� syndicated in 19 cities and featuring celebrity guests willing to be prodded about sex and other risque topics.
She also went on to write five more books, including �Having It All� in 1982 and in 1993, at age 71, �The Late Show,� which was subtitled: �A Semiwild but Practical Survival Plan for Women Over 50.�
�My own philosophy is if you�re not having sex, you�re finished. It separates the girls from the old people,� she told an interviewer.
The Browns were childless by choice, she said.
Rayner Pike contributed to this report.
Mark Lennihan/Associated Press


text:
Quadruple Helices Appear To Have Role...
Wall Street Decline Takes Its Toll...
Expanding Use of NGS Has Largely...
As Immunotherapy Revolutionizes the Cancer Treatment...
Quantitative PCR (qPCR) is a powerful tool used for genotyping, SNP analysis, and gene-expression studies. Quantitative detection of small mRNA expression changes facilitates the characterization of early-stage cancer and infectious disease. Advances in qPCR technology to enable the use of high density 384- and 1,536-well microplates have led to a demand for automation and miniaturization of qPCR preparation.
With automation, researchers can generate larger sets of data quickly, while minimizing resource burden and improving reproducibility across assays. As researchers push further toward miniaturization, potential cost savings are met with a high risk for cross contamination, lower precision, and poor accuracy.
Labcyte's Access™ workstation relies on the company's Echo® liquid handler and its tipless, touchless transfer of samples and reagents to eliminate such risks. With the use of acoustic energy, the Echo liquid handler enables cherry-picking and reformatting of samples from any well of a source plate to any well of an assay plate with high precision, accuracy, and no risk for cross contamination.
The Access workstation seamlessly adds automated sealing, centrifugation, and bulk reagent dispensing—creating a simple walk-up and walk-away solution to overcome the challenges of miniaturized, high-throughput gene-expression profiling (Figure 1).
Moving qPCR workflows to high-density formats can provide a valuable increase in experimental throughput. Additionally, by reducing the amount of source mRNA consumed, researchers can dramatically increase the number of experiments performed with limited amounts of starting sample.
When commonly used volumes of 25 µL are reduced to 300 nL, the savings on master mix, probes, and primers are more than 95%. The same expenditure can yield twenty times as many high-quality assays or provide savings of tens to hundreds of thousands of dollars per year.
With a suite of software applications to guide protocol development and automation control, the time required to implement the Access workstation for qPCR workflows is far less than expected. For qPCR setup, the Echo Plate Reformat (EPR) application is used to map transfers of master mix, primers, and cDNA samples from source plates to PCR assay plates. With the freedom of any-well to any-well transfers, the Echo liquid handler accommodates any arrangement of samples and assay reagents for assay design flexibility.
The Access workstation's automation control software incorporates EPR transfer protocols into automated routines with plate-handling actions optimized for efficiency. Since every automation event is scheduled to ensure that the Echo liquid-handler steps are given the highest priority, automated routines achieve maximum throughput.
Wizard-based interfaces make it easy to edit existing protocols, track plate properties, analyze reports, and quickly recover from errors. The guidance and utility of the software alone accelerates transfer of assays from the lab bench to the Access system.
Randy Dyer ([email protected]) is product manager, and Celeste Glazer ([email protected]) is applications scientist at Labcyte.
Randy Dyer ([email protected]) is product manager, and Celeste Glazer ([email protected]) is applications scientist at Labcyte.
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While Zika continues to garner a lot of news coverage, a Mayo Clinic survey reveals that Americans believe the country's most significant healthcare challenge is cancer. Compared to other diseases, does the possibility of developing cancer worry you the most?
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