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Quadruple Helices Appear To Have Role...
Adapting to Evolving Business Environments Is...
Nothing Beats Immunoassays for Sensitivity, Specificity,...
Process Development Jobs Still the Most...
Extraction and purification of nucleic acids from biological materials, whether for research use such as genomic analysis, gene-expression studies, and genotyping or clinical applications like pharmacogenomics, diagnostic and prognostic use, or forensics, involve many of the same challenges. These include the diversity of sample types, the susceptibility of DNA and RNA to nucleases present in cell lysates, and the demand for high-quality, pure nucleic acid suitable for amplification and quantification, use in microarrays, and storage/sample archiving. Samples may range from fresh or frozen whole blood to plasma, buccal scrapes, cell culture, biopsy material, paraffin-embedded tissue, or plant tissue. Researchers at next month's "GOT Summit" CHI meeting, will be discussing new tools and techniques for improving sample prep."For a while now, the trend in nucleic acid sample prep has been the more simple the kit and more simple the protocol, the better for the user," says Steven Dodsworth, Ph.D., director of molecular genetics and genotyping at Tepnel Life Sciences. "If things go wrong in downstream processes, whatever technique you are using, sample prep is something you should automatically suspect. Yet if the user knows little about the extraction and purification process, it may be difficult to troubleshoot the problem." From a product-development perspective, the robustness of an extraction method is paramount, as the more robust the method, the simpler the protocol can be. As users will vary in their technical abilities, ensuring robustness means that even with variation in volume, for example, of as much as ±10%, the prep should still be salvageable. Trying to save on costs upstream during sample prep may have an effect not only on process quality and outcomes but, ultimately, on overall cost.
Tepnel's nucleic acid prep kits are based on the company's Nucleon™ extraction chemistry. The Nucleon HT DNA extraction system can be used to extract DNA from paraffin-embedded tissues or other hard tissues such as mouse tail that require proteinase K digestion for cell lysis. The protocol takes less than 40 minutes and yields more than 40 µg of high purity (A260/280=1.8–1.9) and high molecular weight (>50 kb) DNA per cm of mouse tail, according to Dr. Dodsworth.The Nucleon BACC DNA extraction system can isolate high molecular weight DNA from whole blood and cultured cells, with yields of 370–440 µg/10 mL of blood in less than 30 minutes, reports Dr. Dodsworth. The kit is scalable from 1.5 to 10 mL. Kevin Krenitzky, M.D., CEO of BioServe, highlights the company's capabilities for extracting DNA, RNA, and protein at the same time from a biological sample to help customers go from "biomaterial to validated data."
Strategies to maximize target recovery for nucleic acid detection in clinical specimens will be the topic of a "GOT Summit" talk to be given by Bernhard Kaltenboeck, D.V.M., Ph.D., professor and director of the molecular diagnostics lab, department of pathobiology, at Auburn University. The limited sensitivity of many current assays designed to detect pathogenic agents present in low numbers in biological samples is an ongoing challenge for nucleic acid-based diagnostics, despite the ability of PCR and other nucleic acid amplification techniques to detect single target copies. The main problem, according to Dr. Kaltenboeck, is the lack of robust extraction methods capable of recovering rare copies of target DNA from large sample volumes and concentrating them for use in assay protocols. Dr. Kaltenboeck will present an approach for the efficient extraction and concentration of low copy number DNA targets that can increase assay detection sensitivity by as much as 100-fold compared to conventional techniques, he says. Chlamydia is described by Dr. Kaltenboeck as an example of a sneaky pathogen that causes low-level inflammation but does not kill cells. It has been implicated in heart disease and other chronic degenerative inflammatory diseases. The pathogen is present in very low copy numbers in affected tissue and is difficult to cultivate. The diagnostic challenge lies in capturing the few copies of Chlamydia that are present for detection with PCR in a 20 µL reaction volume, of which only 5–10 µL is sample.Mastitis in cows may be caused by Chlamydia. To test their nucleic acid extraction method, Dr. Kaltenboeck's group spiked cow's milk, a particularly difficult diagnostic medium due to its high fat and sugar content, with Chlamydia and removed the DNA from solution by binding to a glass fiber matrix. Following multiple wash steps to remove the proteins, carbohydrates, lipids, and other cell matrix components, they then eluted the DNA by increasing the pH and collected the sample reservoir. While DNA extraction kits that perform these functions are commercially available, "the standard protocol for their use will result in the recovery of a dilute DNA sample, so that five microliters of the eluted DNA (the PCR input volume) will be equivalent to about one microliter of original sample volume," says Dr. Kaltenboeck. "It is obvious that this limits the detection by even the best PCR method to about 1,000 DNA copies per mL of original sample. This is not sufficiently sensitive for detection of low-level, chronic infections."Dr. Kaltenboeck improved the overall DNA extraction efficiency by combining multiple steps that stabilized nucleic acids, bound more nucleic acids to the extraction matrix, and recovered those nucleic acids in a lower eluate volume. These steps are multiplicative in their effect on improving nucleic acids recovery. A frequently overlooked factor limiting detection sensitivity is the rapid degradation of nucleic acids after thawing of frozen specimens. "This can reduce recoverable nucleic acids by as much as 50 percent within five minutes at room temperature," explains Dr. Kaltenboeck. Addition of an equal volume of guanidinium-based sample-preservation buffer immediately after sample collection stops nucleic acid degradation and allows storage and shipment of samples at ambient temperature, with less than 30% target loss over a one-year storage time, according to Dr. Kaltenboeck. Furthermore, repeated addition of up to seven 200 µL aliquots of the sample-preservation buffer mix, instead of a single aliquot, increases nucleic acid binding by up to sevenfold. Elution in a 20 µL reaction volume instead of 200 µL increased nucleic acid concentration by another 10-fold. "When combined, these steps amounted to 100-fold higher DNA input per PCR than by using standard nucleic acid extraction procedures and increased PCR sensitivity by this amount," says Dr. Kaltenboeck.At the "GOT Summit," Huimin Kong, Ph.D., president and CEO of BioHelix, will describe the company's Rapisome™ pWGA kit, a primase-based whole-genome amplification (WGA) system that can be used for nucleic acid sample prep. WGA is a valuable method of generating the starting material needed for real-time PCR and of expanding a limited genetic sample for archiving, genotyping, forensic analysis, aCGH for genome-wide copy number analysis, or other downstream applications, according to Dr. Kong."pWGA can achieve 1,000-fold amplification," reports Dr. Kong. The technology utilizes a primase enzyme to synthesize primers directly on the DNA template, rather than adding random, ready-made primers to the sample. It performs rapid isothermal DNA amplification without the need for thermocycling or heat denaturation, explains Dr. Kong. Rapisome also incorporates helicase activity to unwind the DNA to enable primer synthesis. "We have reconstituted the in vivo DNA replication system and can do it in vitro in one step in one hour," explains Dr. Kong. The Rapisome pWGA kit's primary research application has been in preamplification for downstream analysis, but Dr. Kong envisions a growing role for the technology in clinical diagnostics. For example, Dr. Kong points to cystic fibrosis diagnostics, which at present rely on simultaneous scoring of 20 mutations. The patient sample must first undergo DNA amplification to generate enough DNA to perform multiplexed SNP genotyping. Multiplex PCR-based amplification has limitations that Dr. Kong believes can be overcome using WGA. BioHelix is working to enhance the amplification power and sensitivity of WGA to enable its future development for highly multiplexed diagnostic applications.
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Fair ~
High: 70°F ~ Low: 50°F
Friday, Sep. 30, 2016
Billie Jean had a tanning bed.Posted Thursday, May 19, 2011, at 9:17 AM
Billie Jean Umbach was born Sept. 8, 1976, at St. Joseph Mercy Hospital in Sioux City, the daughter of Larry and Karen (Jeffrey) Wink. She was raised in Anthon, where as a youth she was a high achieving 4-H member of the Anthon Advancers 4-H club. She graduated from Maple Valley Anthon-Oto High School with the class of 1995. While in school she accelerated academically, was active as a cheerleader and was a member of the color guard, high school band and other school activities. After high school, Billie attended Omaha College of Health Sciences and Hawkeye Community College in Waterloo, Iowa,obtaining her degree in dental hygiene.
On Nov. 23, 2001, Billie married her high school sweetheart, Lincoln Umbach. On June 4, 2005, the couple were blessed with their only child, Keegan LaThomas. The couple enjoyed parenting, taking family vacations and every moment spent together.
Billie was a member of St. Joseph Catholic Church. She enjoyed scrapbooking, photography, golf, gardening, vacationing and bonfires with friends. Billie loved celebrating Christmas and the Fourth of July. She loved cooking and baking, especially decorating sugar cookies for all holidays. She treasured spending time with family and friends and enjoyed playing cards and board games. Above all, she loved her family, especially her beloved husband and young son.
Billie Umbach, 34, of Anthon passed away Tuesday, May 17, 2011, at a Sioux City hospital after fighting a courageous 5 1/2-year journey against melanoma. She was surrounded by her loving family and friends.
Survivors left to cherish their memories of Billie include her husband, Lincoln, and son, Keegan, and his puppy, "Paws
Billie Jean grew up with a tanning bed in her home. She tanned like many do for various reasons. Maybe it won't happen to you. Maybe she was just more susceptible to cancer than others. Maybe her son would have still have his mommy and her husband his soulmate if only tanning beds didn't exist. Sure it could've been a car accident, or another disease or maybe its just God's will and when its your time it just is. But unlike cigarettes or heavy drinking, the perception is that tanning beds make you more beautiful therefore they are allowed. The risks are underplayed. The warnings minimal. Make a choice to be there for your children, your spouse, your family. Its just one more healthy choice to stay away from obvious harm.
Showing comments in chronological order
[Show most recent comments first]
How disprespectful can you get! You didn't even copy and paste her FULL obituary here! You forgot to mention this KEY PARAGRAPH: (these was taken from her FULL OBITUARY in the Sioux City Journal)
After she was diagnosed with cancer, Billie began the Wild Angels Relay for Life team. Now, after 5 1/2 years, the team is more than 200 members strong. She cherished the hours devoted to raising money for the American Cancer Society to aid in the fight for a cure for all cancers. She and her Wild Angels team have raised $70,000 for the American Cancer Society. In 2009, Billie served as the honorary spokeswoman for the Relay for Life event.
-- Posted by GolfGal05 on Thu, May 19, 2011, at 1:27 PM
Um, didn't mean to be disrespectful, just brief as possible. Calm down. That wasn't the point of my blog.
-- Posted by Leah Cauthron on Thu, May 19, 2011, at 1:37 PM
I think the point of this blog was to point out the very tragic yet often overlooked dangers that can come from a product that is used quite regularly by people of all ages. The point of the blog is to encourage persons not to take unnecessary risks in a society that seems to believe, "It could never happen to me." This young lady died way to early. Hopefully someone else can learn from the tragedy.
-- Posted by randy cauthron on Thu, May 19, 2011, at 4:02 PM
This story breaks my heart.
With all the respect in me, I was horrified to find that the Google ad that came up to the right was for a bronzing serum, and it had photos of a pasty white leg next to a tan one to show its effects.
I do agree that tanning beds and tanning in general often get a pass because "of course you can't go through life pasty." The only skin cancer I had been familiar with was the kind that was fairly easily removed. It's only more recently that I've read that like any cancer it can move inside you and affect your lymph nodes, liver and lungs.
Even if it just ravages your skin, skin is your largest organ and it protects everything else. If it's compromised you're opened up to all kinds of infections.
I'm very sorry to the family and friends for such a great loss.
-- Posted by AmyPeterson on Fri, May 20, 2011, at 6:26 AM
Billie was a brave, courageous, amazing woman who not only fought this brutal disease with everything she had, she also educated others along the way and helped raise money for cancer research. She will be forever missed by those who loved her and were fortunate enough to have met her and see her infectous smile.
I understand you were trying to make a point and use Billie as point of reference on the affects of tanning beds and cancer, however I feel you could have taken a different approach to it. If you thought how her family would feel if they read this, you maybe wouldn't have gone about it the way you did. Posting this before they were able to say their final goodbyes maybe wasn't the most sympathetic approach.
-- Posted by cyfan on Fri, May 20, 2011, at 9:14 AM
I apologize and want to re-iterate that this was not written in any way to be disrespectful or to place blame on Billie Jean. This is about an industry that needs to be taken to task for harming a society in the name of a perception of beauty. The obituary was beautifully written and I felt that the only way to change people's perceptions was to make it personal. Help them to see themselves in Billie Jean and hopefully her story can still save lives.
-- Posted by Leah Cauthron on Fri, May 20, 2011, at 9:44 AM
Thanks for posting Leah. The real lesson can be posed as a question: How much personal risk are you willing to take in order to look good or feel better temporarily? Too much tanning in a tanning bed obviously increases the risk of getting skin cancer. It's extremely disheartening to hear of this very young woman's death.
-- Posted by bighops85 on Fri, May 27, 2011, at 10:55 PM
Thanks for covering this subject.We lost our daughter 7 years ago to this desease and she also left behind loved ones including 3 young children.
Although she did occasionaly tan she did not blame tanning for her desease.She did however belong to an online support group and many of the girls in the group beleived the tanning booths were responsible for their condition.Ironically 6 months after her death my wife happened to visit this support group web site and someone was asking if anyone knew what happened to our daughter,she replied and we received many responses letting us know that our daughter had helped them with her upbeat blogs during her ordeal.
-- Posted by vikefan on Sun, May 29, 2011, at 9:38 AM
-- Posted by Leah Cauthron on Thu, Jun 9, 2011, at 8:57 AM
As Billie's cosuin, I think it would be very polite of you to delete this post. The last thing people need to read, when Google searching Billie, is to find this article.
Perhaps you can take a different approach on this serious topic and use statistics rather than pin pointing individuals. Although she was taken away from us far too early, she accomplished so much in life that many could only hope to do. This year our Relay for Life Team, The Wild Angels, have hit the $85,000 fundraising mark over the last few years of fundraising.
I challenge you to come to the Relay for Life this year in Sioux City, and write an inspirational/positive "blog post" quoting the opinions that come from the relay participants about coming together and fighting for a cure. You can start by asking me, as I will be in attendace.
-- Posted by CodyUmbach on Tue, May 15, 2012, at 4:45 AM
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My name is Leah Cauthron. I was born and raised on a farm near Cushing, Iowa. My parents still live there. I am a conservative Catholic. I have been married to Randy for 22 years and we have six children. I received my degree in Criminal Justice from Eastern Wyoming College and as a mother of six and a daycare mom, I have used probable cause, reasonable doubt, search and seizure, Miranda rights, submission, arrest and interrogation technique as a part of my daily life. I can also shoot a .357 magnum with deadly accuracy. I have lots of opinions on lots of things. I am not an expert on anything but I read a lot and will gladly share that information if I find it accurate. I have a sarcastic nature and for the most part I have pretty thick skin and most things don't bother me for long. I don't like mudslinging or name calling so keep negativity of the personal nature to yourself. Enjoy and welcome to my opinion.
Hot topicsTake from the Soliders....again.
(2 ~ 1:56 AM, Feb 18)
Share the Road and Be Safe
Family Vacation
Circle of Safety Check

subset: commoncrawl


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Sandy hurts Delta's November revenues by $30 million, profits by $25 million
Hurricane/Superstorm Sandy reduced Delta Air Lines' November revenues by about $30 million and its profits by about $25 million, the airline said Tuesday.
With significant operations at New York area airports, Delta was among the major carriers that saw a big impact from Sandy, which closed down a number of East Coast airports in late October and affected bookings and rebookings into November.
Delta said its unit revenues – revenues per available seat mile flown – increased 2.5 percent over November 2011, but "was approximately one point lower than it would have been without the storm impact."
On Nov. 26, US Airways said Sandy caused a $30 million decline in October revenues and a $15 million drop in October income.
In her Monday weekly note to investors, airline analyst Helane Becker of Dahlman, Rose & Co. singled out Delta, US Airways, United Airlines, Spirit Airlines and JetBlue Airways as the carriers with the most exposure to the storm. She summed up the situation in a headline: "Sandy's Slow Torture."


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Craftsman 255 pc. Mechanics Tool Set with Lift Top Storage Chest
Craftsman 255 Pc Mechanics Tool Set with Storage Chest: Tool Around at Sears Nothing is more frustrating than finding yourself without the proper tools right in the middle of a simple or complex vehicle maintenance project--but with the Craftsman 255 pc Mechanics Tool Set with Storage Chest , that will never happen again. The super strong tools include wrenches, a slew of sockets that are compatible with three quick release ratchets and 62 specialty tools. From simple tasks like battery replacement and tune-ups to pulling engines and brake replacements or other projects around the house, this set will never let you down. With the 255 Pc Mechanics Tool Set , you'll never have to borrow tools from neighbors or friends; they'll be coming to you for help! And there's no worry about the tools breaking because they all come with a lifetime guarantee. If they ever break--which is extremely rare--your local Sears store will replace them, no questions asked--ever. You'll have to buy many cars in a lifetime, but these tools last forever.
- The Craftsman 255 pc Mechanics Tool Set with Storage Chest includes 1/4, 3/8 and 1/2 in. drives with 158 sockets, 3 quick-release ratchets and 4 extension bars, 28 wrenches up to 3/4-in. and 17 mm sizes and 62 specialty tools
- All tools are cast from high quality steel
- Tool kits come ready to use right out of the box for projects that need immediate attention
- Backed by Craftsman's famous Forever Warranty
- Comes with a sturdy tool chest with 3 pull-out drawers with a tight-fitting attached lid


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Top Definition
LolZzZZz is just another way of saying LOL or the very simple LOLZZ but in a more extreme way . It shows more of histerical laughter then a simple "hehehehahaha" . Actully , this internet slang was created by a typo .
Chad: Hey Chrisy <3 , holy shit i jus got pwned
Christina: LolZzZZz hehehe
Christina: LolZzZZz hehehe
автор: ChadzGirl14 20 июня 2009
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resource categories
- God's Way - Leaders: The special teaching responsibility of ordained leaders within the every-member ministry of the body of Christ, and the need to provide for its continuance.
- God's Way - Women: The unique value of women's ministry in the local congregation but also the divine order of male headship, which makes the headship of women as priests in charge, incumbents, dignitaries and bishops inappropriate.
- God's Way - Marriage: The vital importance of monogamous life-long marriage for the care and nurture of children, and the well being of human society.
- God's Way - Sexuality: The rightness of sexual intercourse in heterosexual marriage, and the wrongness of such activity both outside it and in all its homosexual forms.
- God's Way - Church Governance: The urgent need for decentralisation at national, diocesan and deanery level, and the need radically to reform the present shape of episcopacy and pastoral discipline, to enable local churches to evangelise more effectively.
- Reform Conferences: Downloads from recent Reform Conferences
What is Reform?Download: originalreformpaper.doc Print this resource
What is Reform?
[The text of the original leaflet]
For some years groups of mainstream evangelicals have met to discuss issues in the church and nation. These issues include the authority and sufficiency of Scripture; the uniqueness and finality of Christ; the priority of the local church; the complementarity of men and women.
Representatives of these various groups, all parochial clergy, met together on 22 February 1993 to pray, to discuss and to consider appropriate action.
As Christians first, Evangelicals second and Anglicans third, they would all affirm the Anglican faith as expressed in its historic formularies. They committed themselves to unite for action under the authority of Scripture as God's Word written, as expressed in the Chicago Statement (to be found in "God Has Spoken", J I Packer, Hodder & Stoughton).
Out of that meeting was started Reform.
Need for change
Historic Anglican theology is committed to continuous Reformation of the church. We are committed to the reform of ourselves, our congregation and our world by the gospel.
We are thankful for the Christian heritage which God has given us in the UK.
- The majority in our nation still "identifying" with the Christian faith
- The resources and property of our national church given for gospel purposes
- The relative freedom to preach the gospel
Reform, however, is urgently needed.
- The gospel is not shaping and changing our church and our society: our society seems to be shaping and changing us
- A biblical Christian voice is heard too little in our society
- The Church of England seems to have lost confidence in the truth and the power of the gospel.
- Active participation in church life is declining (during the last decade Anglican membership decreased by 15%)
- National moral and social values are therefore being eroded
To reverse these trends we are committed to change and growth.Such change will be costly.
Aim - Evangelisation of the Nation
To honour Christ as Lord and Saviour through:
- Prayer and the preaching and teaching of the apostolic gospel
- The Christ-like living of the people of God
- The recruiting, training and deploying of ministers of the gospel
- Church growth and church planting throughout the UK
- The release of money and people to these ends
We do not believe this change can come from the denominational centre. The local church must take back responsibility for the denomination.
Initiatives must come from local parishes and congregations and are already being taken.
We are committed to:
Pray, study God's Word and take action together.
The reforming Process is to change:
- Ourselves
- Our churches
- Our denomination
- Our nation
- Our world
How does it work?
- By establishing a network of individuals and affiliated churches
- By encouraging groups and conferences where members can pray, think, study and come to a common mind from which they can take joint action
- By publishing discussion papers and circulating the membership with ideas, news and developments
- By facilitating action on our aim
How can I play a part?
Reform is open to all who are willing to pay the price of:
- Prayer
- Time
- Work
- Thinking
- Changing
- Facing criticism for changing
- £25 (£30 couple or £10 Student/Pensioner) to join the network [nb: original subscription levels]
The [original] Council members:
Donald Allister, Mark Ashton, David Holloway, Tony Baker, Dick Lucas, David Banting, Wallace Benn, Ken Moulder, Mark Burkill, Hugh Palmer, David Fletcher, John Pearce, Jonathan Fletcher, Alex Ross, Miles Thomson, Chris Green, Melvin Tinker, Philip Hacking.
Reform, as a grass roots movement, envisages action coming from members who have thought, studied, discussed and agreed. All these web resources are in keeping with the Reform Covenant but the individual contributors alone are responsible for the content.


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Zoe Bake's Brown-Butter Banana Beehive Cakelaurasrecipes
Acclaimed pastry chef and best-selling cookbook author Zoe Francois has a fabulous blog that features wonderful desserts and baking recipes. Her site is one of my favorites as everything is not only delicious but beautiful. Yesterday she posted the most adorable beehive cake with little marzipan bees floating around the cake. It's beautiful and precious… oh.. and the cake? It's a brown-butter banana cake with honey buttercream…. swoon!
As a lover (and keeper) of honeybees and a firm believer in natural foods like honey…. I loved this cake. Zoe states it's a "tribute to both the elegance and ingenuity of honeybees." Unlike many other baking sites, Zoe takes the time to post step-by-step instructions that make recreating her recipes a snap. Your beehive cake will has a good chance of looking just like Zoe's cake. To see how she did it, please visit her original post by clicking this link.
Photo credit: Zoe Francois – zoebakes.com


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Top Definition
Golden Gordon (n) - an occurance involving Alex Goldwater (Gordon) making a fool of himself and not realising, causing much amusement to all about.
Gordon holding a cup of coffee, then being asked the time. He proceeds to turn his wrist to look at his watch, simultaneously pouring coffee all over himself.
بواسطة Mitch سبتمبر/أيلول 2, 2004
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From You Flowers Coupons and Promo Codes
From You Flowers delivers flowers for any occasion. They work with over 20,000 florists around the world to make sure your flowers are fresh and delivered on time. From You Flowers has been quoted by CNBC as,"Best Overall Quality & Price." A From You Flowers coupon will take a percentage or flat rate off of your final purchase. And if you are in a hurry, same day delivery is an option!
Save on those floral blooms with a From You Flowers promo code. All types of flowers are available, roses, lilies, orchids, tulips, daisies and more! For all occasions such as birthday, get well, sympathy, funeral, graduation, etc. There are plants for purchase too. Give a gift that keeps growing! Examples include blue hydrangea, succulent basket, zen planter, and Azalea Bonsai. Or you'd prefer to give a gift of candy, snacks, or fruit, From You Flowers has that too!


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on February 6, 2013 - 11:25 PM
, updated February 7, 2013 at 12:17 AM
Here's a challenge for high school boys basketball followers: Go to a game in Erie County and try to find someone on the court whose basketball life hasn't been forged by the Depew Boys & Girls Club.
It likely will be tougher than a fadeaway three under heavy defensive pressure, tougher than trying to figure out when to bench a star who is in foul trouble, tougher than making the block/charge call. That's because the club has produced a litany of outstanding players, coaches and officials whose impact is felt on Western New York basketball pretty much any day there are games being played.
It is a club that is now in need of some payback for all the lives it has affected after a devastating New Year's Day fire. The Depew club on Preston Street was gutted and will need extensive repairs. Management at the club, which has relocated temporarily to Terrace Elementary, intends to rebuild on the original site.
The club is holding a fundraiser Saturday evening at St. Mary's High School's fieldhouse. A $25 ticket includes food, beer, soda and music; doors open at 5:30 with the event running from 6 to 11 p.m.
There are sure to be many familiar faces on hand – the same ones seen in local gyms every night. The club helped players and coaches hone their games, or gave coaches a place to keep playing the game they love. The officiating in the club has its own special family tree, with two of its prominent members now leading the club: Executive Director David A. Hoch and Program Director Jeff Kupka.
"I doubt I have ref'd a game in 17 years that at least one person in the gym didn't have a connection to our club," Kupka said. "I was at Will North last week and a fan approached me that I played against at the club years ago."
And the connections. Where to start? How about with the plaque that denotes the "Youth of the Year" at the club. Those winners include Frank Payne (former football and current girls basketball coach at Iroquois) and his brother Chris (football coach at Panama), Dan Gill (former St. Mary's athletic director and coach who is now at University School in Florida), Jeremy Konst (Williamsville East standout and current Williamsville North JV coach and an official) and twin brother Justin Konst (Will East All-WNY backcourt mate of his brother and current official).
The club's most famous alum is Depew native Don Majkowski, who went on to play quarterback for the Green Bay Packers (and who donated the club's glass backboards).
Pete Jerebko, who grew up at the club, starred at Le Moyne and is currently the athletic director at Erie Community College and also an official.
Hoch started the club's men's leagues in 1978 and its high school summer leagues in 1989.
The amount of area coaches who have played in the men's leagues borders on ridiculous: Mark Kensy (Maryvale) and sons Chris (Amherst) and Garrett (Maryvale assistant who played with the club since the age of 11), Mike Haskell (former Pioneer, current D'Youville) and son Jeff Haskell, Jay Robbins (Kenmore East), Chuck Swierski (Williamsville North and a playing standout at Buffalo State), Ryan Mountain (Maryvale grad coaching North Tonawanda), Kyle Husband (Canisius), Paul Rath (St. Mary's), Luke Vogel (North Tonawanda girls), Gary Schutrum (former East Aurora boys and current girls coach), Mark Rydza (Roy-Hart), Tim Schulz (Holland), Sean Lauber (Iroquois), Dave Rappl (Hutch-Tech), who grew up coming to the club with brother Mike Rappl (Canisius College softball), Justin Gerstung (Akron coach played during his high school days), Jack Herlan (St. Joe's assistant), Modie Cox (former UB standout who currently works with youth).
And those are just the names I know from my 14 years covering high school sports. The list is unbelievable and way too long for this space — go to the Prep Talk blog for even more alums.
Hoch – one of the area's very best officials – takes tremendous pride in those who have taken to the court as officials later in life.
"There has been a large number of club 'kids' who have followed me into officiating in Board 53" of the International Association of Approved Basketball Officials "and the collegiate ranks," Hoch said.
That list includes current Division I official Kevin Ferguson and highlyrated current officials, in addition to Hoch, like Kupka, Steve Allen, Mark Clifford and Mike Nagorski. All got their start officiating men's or youth games at the club.
To make a donation to the rebuilding fund, to purchase tickets or for more information, contact the club at 683-6755 or 683-1485, email Hoch at email@example.com or visit the club's website at depewlancasterbgc.org.
Signing day
The only local high school player to commit to a major Division I program on national signing day was, as expected, Canisius lineman Ryan Hunter signing with Bowling Green. Other local seniors to commit to Football Championship Subdivision (formerly Division I-AA) programs are Canisius wide receiver Denzel Benton (Rhode Island), West Seneca East quarterback Andy Smigiera (Robert Morris) and Orchard Park lineman Michael Senn, who has already begun taking classes at the University at Albany.
email: email@example.com


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The following documents are required by CTC's Bikeability providers. These are available for download below.
- A consent form for every child before they start their first session.
- A register - to be completed every session.
- Three risk assessments; a generic risk assessment, and a risk assessment for the Level 1 area and the Level 2 area.
- The Outcomes Document to help you plan and track your sessions and finally,
- An accident form (if one should arise)
If you have any questions at all about these forms, then please do not hesitate to contact us email@example.com
Work for CTC
CTC needs instructors to deliver its Bikeability schemes. In some areas this is via a contract with the local authority, working in schools. In other areas it is via Bike Club. Bike Club aims to bring cycle training to children who, for whatever reason, did not received their Bikeability training in Year 5 or 6. CTC also needs instructors for other activities and events.
One of the first places we will look for instructors is on the training section of CTC-Maps, so do keep your entry up-to-date.
Cycling Training Helpline 0844 736 8460


text:
Jehovah's Witnesses - Kingdom Halls- New Britain South telephone number is (860) 223-0893.
Telefax: No fax number available
Website: No web address available
Biz owner? Moderate reviews or edit listing.
Search entire category for Churches
Churches, in South Windsor, are buildings for Christian congregation and worship. The clergy disseminate teachings of Jesus Christ in these Christian churches.
Governor Cuomo Announces Growth Of Clean Technology Companies And Creation Of 980 Jobs
$14 million Investment in Cleantech Business Incubator Initiative Supports the Development of 141 Clean Energy Companies across the State (Long Island, NY) Governor Andrew M. Cuomo today announced that 141 clean technology companies have been supported and primed for further growth through the Governor's Cleantech Business Incubator initiative. The initiative has attracted more than $215 million in private capital..


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Top Definition
persistent self-diagnosis from an unlikely combination of symptoms found only in the world's most unreliable dictionary.
"Oh no! He's not 'phoned in again on a sickie, has he? What's the flakey git found out he's suffering from now? I reckon he's got a severe case of the wikichondrias."
JJJJJimmyP가 작성 2009년 09월 24일 (목)
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text:
UNIVERSITY PARK - There's a good bit of irony and also potentially some concern in Bill O'Brien's idea to have two of his top defensive backs play some linebacker this season.
Let's backtrack to a year ago, when John Butler, then Penn State's defensive backs coach and currently the defensive coordinator, made a very telling statement about what he inherited in the secondary.
"I know a lot of the readers out there are wondering why we don't play nickel, and I guess I'm wondering why I only have six defensive backs when I took the job here," Butler candidly said last October.
He was speaking of how there were only six DBs on scholarship, which of course limited what he could do from a scheme standpoint. The Nittany Lions, in fact, were so limited that they couldn't play nickel coverage all season.
The good news is that's no longer a problem. There's much more depth in the secondary this year, and the unit features arguably the best overall player on the team in junior safety Adrian Amos, along with some other experienced veterans and talented youngsters.
Amos is so good and so versatile that O'Brien plans to use him all over the place - safety, cornerback and even linebacker.
"It makes me feel good because he trusts my ability to play those positions," Amos said. "But also I have to be prepared and make sure I do know all those checks and coverages and everything and learn the techniques of each position."
Senior safety Stephen Obeng-Agyapong also will be in the mix to play some linebacker, O'Brien said, and he can't wait for that opportunity.
"I would enjoy that a lot," Obeng-Agyapong said. "I enjoy hitting, so that's a primary asset of the defense. For me to be at Linebacker U playing linebacker, I'm excited about it. I might not be the biggest linebacker down there, but trust me, I'm not going to let anything get by me."
The fact that Amos and Obeng-Agyapong are versatile enough to handle their own positions plus potentially spend time at linebacker is a testament to their talents.
It's also a testament to how different things are this year in terms of options provided by the secondary.
The bad news, though, is what it says about the Lions' situation at linebacker. Penn State has had a remarkable run of success there in the past decade - producing star after star with such players as Paul Posluszny, Sean Lee, Navorro Bowman and Dan Connor - but the linebacker depth is as weak as it's been in a long, long time this year.
Just because Amos and Obeng-Agyapong could handle things at linebacker doesn't mean they are best suited for that role, and it's not exactly the best-case scenario.
Then again, what's happened to the program in the past two years has been far from the best-case scenario, so O'Brien is doing what he always must do, which is adapt and make the best of things.
In this case, it's about getting the best football players on the field, and if the backup linebackers aren't good enough to be out there, then O'Brien at least has the option to turn to guys like Amos and Obeng-Agyapong.
"You never know," Obeng-Agyapong said when asked how much time he and Amos could spend at linebacker. "It depends on the flow of the game, the team we're playing. You could be there a lot, you could be there minimal times."
Amos pointed out he won't be playing a traditional linebacker spot, but more of an extra linebacker/DB in a nickel package. In that scenario the Lions would be in a 4-2-5 scheme, which is something that could be on display quite a bit this year.
"I don't think [O'Brien] was really saying I was going down to play linebacker," Amos said. "We play a lot of nickel packages and things like that, so I'm taking out a linebacker and playing that position, but I'm covering the receivers in slots."
Amos played cornerback last year and is moving to safety this season. He's been asked so frequently which position he likes best that he has a stock answer.
"People ask me that question, I just say defense," Amos said. "I think I do good things at each one of them.
"I think [cornerback is] the easiest thing to do," he added. "You're locked in, and all it is is just you and him. At safety you get the reins to do a lot of stuff, anything you want, you can disguise more. And then coming down in the box [at linebacker] you get to show your ability to hit the run."
Sophomore Trevor Williams is slated to start at cornerback, impressive since he began his career at receiver and has adapted quickly to defense.
"For him to come from offense to defense like that playing the college level, and for him to make that an easy transition was really good," Obeng-Agyapong said.
Redshirt freshman Malik Golden also switched from receiver to defense and will compete for time as a backup safety.
Senior Malcolm Willis and junior Ryan Keiser are battling to start at the other safety spot, while sophomore Jordan Lucas is the other cornerback. Sophomore cornerback Da'Quan Davis also figures to be in the mix.
Penn State had so little depth in the secondary last year that it not only limited what the team could do in games, it also limited how much the top players could improve in practice.
"Having guys you can compete with every day brings your game to the next level," Obeng-Agyapong said. "It's better for the team as well because if somebody goes down, you always have that next guy to step up and you feel comfortable with them."
"Everybody's getting pushed and held accountable," Amos said. "Even if you're not threatened for your spot, you're still being competitive and playing every day."
Amos isn't threatened for his spot, but watching how many spots he plays this season and how effective he is at each one will be a big key for Penn State's defense.
The even bigger key, compared to last year, will be the sheer number of bodies available to play different positions in the secondary.
"We have a lot of DBs this year and depth in the secondary," Amos said.


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It was just more than a month ago that Harry Styles was helping then-girlfriend Taylor Swift celebrate her birthday with a quiet trip to rural northern England.
But now that he's single, the One Direction hunk opted for a less low-key celebration to mark his 19th birthday. And instead of hanging out with a girlfriend, he got up close and personal with a very, very different lady: a stripper.
Hitting a London club for a Friday night celebration with his friends, including bandmate Niall Horan, the racy rendezvous was a gift from Radio 1's DJ Nick "Grimmy" Grimshaw. And it was more ridiculous than racy, Styles tells the U.K.'s Sun.
"It was great," he said. "I was in stitches – it was really funny."
Added Horan on Twitter: "Was a right laugh."
In character as a cop, his birthday present gave him a lap dance, reports The Sun, before playfully arresting him. Adding to the hilarity: "The funniest part was that when she finished she said, 'I'm really sorry. I couldn't find my truncheon, so I had to bring my nunchucks.' That was a bit weird," he said.
But the festivities weren't quite as X-rated as it sounds. "She stripped down to her underwear," according to Styles, "but unfortunately there was a no-nudity policy in the bar."
The teen idol partied until 3 a.m. before heading to a friend's house. "Put it this way – it was a long day," he said. "We had two nights on the trot, but you have to on your birthday, don't you?"
Harry Styles Celebrates 19th Birthday with a Stripper
Weir Photos/Splash News Online


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Corporate Training & Continuing Education
A Department of Extended Programs
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- Phone: 901-333-4207 • Fax: 901-333-4519
Our Mission
To foster workforce development in the region through a comprehensive continuing education program that consists of customized corporate training, career training, lifelong learning opportunities and business-education partnerships.
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Tuesday, July 15, 2014
Well I must admit that I have been surprised at the amount of response, both pro and con, that I have received regarding my comments on the play Rent.
Just for the record, I do want to clarify what I did say and what I did not say. First, I did not say nor do I feel that I in any way disapproved of the message and general content of Rent. I do not. The theme of the play is as relevant now as when it came out on Broadway. Although the content is disturbing, raising awareness of the many issues such as this is needed. Awareness of issues such as AIDS, teen pregnancy, abuse in many forms, child trafficking, abortion, etc is critical if we are improve our society in general and be able to help those who may be trapped in one of these environments.
What I did say and still do say is that some of the language in the play was crude, vulgar but most of all unnecessary and, in my opinion, detracted from the important message of the play. I fully agree with Mayor Collins in saying that I do not feel that the FPT needs to or should justify why they chose this play in their series. That is looking backwards which generally brings nothing positive but can sometimes be instructive going forward which is where focus needs to be centered.
Some have said that what I want is censorship of what the FPT chooses to have in the theater. Nothing could be further from the truth as free speech needs to remain a founding principal for us all. What I would hope might come of this is dialog going forward about the type plays that would meet the vision and values of the FTP, our Town and the community at large to be most beneficial to all who live here. Like it or not, we are a large bedroom community that is now in the midst of incredible growth in many sectors that is going to be beneficial to our growing citizenry and the businesses we have and the ones soon to come. Working together, I believe through open communication and shared goals that we can find common ground that meets the desires of the FPT and would allow a larger percentage of our populace to be able to enjoy the plays that are aired.
In the case of the message of Rent, my belief is that greater awareness of the message presented is also, maybe most, important to the youth of our community which I feel some of the language did not allow. The Town has provided funding to help the FTP continue to be the vital part of our community that it has been these many years. Providing funding, in my opinion, also gives Town Council or anyone else the right to provide input to the FTP regarding their operations. Hopefully this input can be positive and helpful, but not censorship. I recognize that some of the public may still not agree with my position. As an elected official, I am expected to represent the people and make the best decisions I can based on my knowledge, beliefs and the input I get from others on a myriad of issues. I try my best to do this but realize that I cannot and never will be able to satisfy 100% of the people 100% of the time despite my efforts to do so. I will continue to provide the best leadership I can. I trust this letter has made it clearer as to what my statements actually were and the intent behind them.
I am certainly open to having dialog with anyone regarding this issue as well as any others that involve the Town of Summerville. Thank you to all who have responded on both sides of this issue, it has been truly enlightening.
Terry Jenkins
Summerville Town Council
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The actor, who has had roles on Homeland, Rescue Me and Third Watch, married Yumilka Valerio on Friday in a private ceremony in the Dominican Republic.
"This is the happiest and most blessed moment in our lives," the couple tell PEOPLE in a statement.
The pair said their vows at sunset while on the beach near the Lifestyle Holidays Vacation Resort in Puerto Plata. Raffaele Pitrella designed both the wedding gown and Perez's cream-colored suit. The bride's impressive veil had embroidery detailing.
Perez, 45, and Valerio, 27, have been dating for a year and this is the first marriage for both. The couple are currently honeymooning in the Caribbean.
Alexander Luperon


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text:
Originally posted on LaxSpot on 06/08/2008
What's up ladies and gentlemen?! Well it has been a while since I have posted so I have a lot to tell you about. The last time I wrote I believe I was coming off my first game back and I had a subpar afternoon at the X. I also told you that I expected to rebound pretty strong. We had a bye week and then headed out to Denver to take on the high powered Outlaws, and high powered they were. Jeff Snider (their face off man, and the most recent MVP of the world games) definitely makes the short list of face off men who I truly respect, I rank him in the top three or four, I have ever played against. To tell you the truth I went into halftime 4 and 8 (NOT GOOD) but I went in at half time and refocused. We went back and forth a few times and then I finally found what I had been looking for since the first game…RYTHYM. I was able to rattle off 13 wins in a row, it felt great. Unfortunately it made no difference in the game, we lost 19 to 11 and even though I came out on top of the face off battle, my first half performance inevitably led to my being sat down for this past weekend's game against Boston.
As many of you may know New Jersey Drafted this kid Brennan from Syracuse, and I am sure you were all thinking, I must be Pi$$ed. Weeellllll I was, and this past weekend they played him to "see how he did against a common opponent"…here is the kicker, Jenkins who is the starting X man for Boston and the "common opponent" would not be playing in our second game. Instead good friend of mine, Ben Defelice would be taking the draws. Even though I know that Ben is a fantastic face off man, I also know that he does not regularly take face offs. In short I was real nervous, but I should have had more faith in my friend, because he dominated at the X, It was unreal.
Although I was pleased to see that my spot should be secure, it is bitter sweet. What is disappointing is that I know I could have helped us with that game since face offs played a major part in our loss this past weekend. Our offense was forced to push the issue, so to speak because we almost never had the ball in the second half, so when we got it on offense we were really forcing stuff. I also have to say that although it seems like my spot is secure I actually feel bad for this Brennan kid, to be honest he is a real nice dude, and I think he could be pretty good in this league, he just needs to be eased into the game. I think face offs in this league are one of the hardest transitions to make. EVERY face off man in this league is great, not good, GREAT, and realizing that is the first step in becoming one of them. Since it is likely that I will hang up the cleats if I get through this season in one piece, I think this kid could really learn from my experience. One of the things that I do really well is teach facing off, and it would be really smart if this kid would open his eyes and ears and let me pass along my knowledge of the game. Don't get me wrong I have not decided if I will play next year or not but I am seriously considering hanging them up. My goal was to get back on a team and have a successful year, and if I succeed in doing so I would be fine with handing over the reins to this youngster.
Anyway I expect to be back in the lineup this week, and it should be a real good match up too. We play Rochester and some of you already know this but I actually coached Alex Smith in high school. His father found me and contracted me out to work with Alex. Alex is a carbon copy of me and my style, only he has a few more tricks up his sleeve and youth on his side. This will be like Mr. Miagi and the Karate Kid squaring off for the first time…I like to believe my wisdom will overcome his youth! As always I will let you all know how it goes…wish me luck!
Wax on Wax off!


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Serves 4 |
Prep Time 20 minutes |
Cook Time 25 minutes
1 cup Eden Quinoa, washed and drained
2 cups water
1/8 tsp Eden Sea Salt
1/3 cup Eden Dried Montmorency Cherries, coarsely choppedor Eden Dried Wild Blueberries or Eden Dried Cranberries, do not chop
1 tsp ground cinnamon
1/3 cup organic maple syrup
2 cups Edensoy Vanilla
1 tsp pure vanilla extract
1/3 cup slivered organic almonds, dry pan roasted for garnishor Eden Tamari Roasted Almonds, chopped
Place the quinoa, water, sea salt, dried cherries and cinnamon in a medium saucepan. Cover and bring to a boil. Reduce the flame and simmer for 15 minutes. Add the Edensoy and syrup. Simmer, uncovered, for another 10 minutes. Turn off the flame and mix in the vanilla. Cover the pan and let sit for 15 minutes to thicken. Place in serving bowls and garnish with slivered almonds.
American, Breakfast, Condiments, Dessert & Pastry, Edensoy, Fruit, Gluten-Free, Good/High Fiber, Good/High Protein, Low Sodium, Macrobiotic, Quinoa, Snacks, Vegan, Wheat-Free, Whole Grain, Whole Grain


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CRYSTAL BAY, Nev. - The Kottonmouth Kings are more than just a group, press materials boast - they're a legendary institution ingrained into the fabric of the underground independent music scene. They've transcended labels and stereotypes to become pioneers and vanguards that have helped inspire and revolutionize the music industry.
The Kottonmouth Kings never conformed to the slavery mindset of the exploitive shallow confines of the music industry. They created their own industry.
Led by founder Brad "Daddy" X, and made up of members D-Loc, The Dirtball, Johnny Richter and DJ Bobby B, the group has been on the frontlines of a revolution that's changed the music industry.
Shunned by the mainstream press and media outlets, blackballed for their radical lyrics and controversial stances on legalization, politics and social injustice, the Kings have managed to stand the test of time for more than 15 years.
Musically, The Kings' production has always been overseen and directed by mastermind Brad "Daddy" X, who has pushed the Kings to evolve by infusing hip-hop, punk rock, and melodic sonic layers of original music.
What sets the Kottonmouth Kings apart from the pack, and makes them such a dynamic and complex group, is that all its members have released solo records and side projects. This musical freedom allows the members to display and evolve the depths of their individual talents and range.
"The Kings have the greatest fans and family in the world, and they validate our music! We have a connection with each other, an understanding, sense of belonging, and a common ground," Daddy X said. "That is a very powerful and amazing gift that allows a group to bring people together and inspire and uplift with music. We are beyond blessed."


text:
Top Definition
poorest country in the world, sitting in a very rural and rugged part of africa. nobody from the outside world knew the region existed til the mid-1800s. the french exploited this place until the 1950s. since then it's been extremely unstable.
the central african republic should be called central african anarchy.
ayon kay Ben E. Hama ika-01 ng Pebrero, 2007
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TB4HB EC Gas Leaf Blower
25cc, 4-Cycle Full-Crank Engine Gas Leaf Blower
Clean up fall leaves quickly and easily with Troy-Bilt's TB4HB EC gas leaf blower. Or use this powerful, electric-start capable 4-cycle leaf blower in the spring and summer for all-purpose clean-up of yard debris, dust and dirt on decks, sidewalks, etc. The TB4HB EC features a 25cc, 4-cycle engine with up to 150mph airflow velocity.
Signature Features
- No Mixing Oil and Gas
- SpringAssist™ Starting Technology
- JumpStart™ Capable
Additional Features
- Variable speed settings allow you to adjust to your clean-up
- Blowing speeds up to 150 mph / 450 cfm airflow
- Over mold handle reduces operator fatigue
- 25cc*, 4-cycle engine eliminates the need to mix gas & oil
- 2-year limited warranty
*As rated by engine manufacturer
- Engine / Motor
25cc, 4-cycle
- Starting System
SpringAssist with JumpStart™ capability
- Choke System
Slicer choke
- Speed Settings
- Airflow Velocity
150 mph
- Airflow Volume
450 cfm
- Included Accessories
- Handle
Over mold
- Warranty
2-year limited
Operator's ManualsDownload operator's manual (PDF)
WarrantyDownload warranty (PDF)
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*as rated by engine manufacturer


text:
Creating Search-Based Solutions with SharePoint 2010 by Scott Hillier
- Posted: Nov 05, 2010 at 10:47AM
Right click "Save as…"
Search-based solutions are applications that use a search page as the primary interface. Solutions such as image searching or travel searching in Bing are good examples of search-based solutions. SharePoint 2010 offers developers new ways to extend search and create search-based solutions. In this session, attendees will learn to create search-based solutions by using custom relevance models, extending SharePoint 2010 search parts, and utilizing .NET Assembly Connectors to access external systems. The techniques presented will prepare attendees to create search-based solutions on their own.
This session is presented by Scott Hillier
Scot Hillier is an independent consultant and Microsoft Most Valuable Professional focused on creating solutions for Information Workers with SharePoint, Office, and related .NET technologies. He is the author of 10 books on Microsoft technologies including Microsoft SharePoint: Building Office 2007 Solutions in C# 2005. Scot splits his time between consulting on SharePoint projects and training for Critical Path Training (a joint venture of Ted Pattison and Andrew Connell). Scot is a former U. S. Navy submarine officer and graduate of the Virginia Military Institute. Scot can be reached at email@example.com.
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text:
Top Definition
A silly insult used for someone who says something stupid, a person who is really ditsy, or someone with an abnormally sized/shaped head. It's used between friends and not taken seriously.
O'Reilly thinks that Sophia, Nicole, and Felicia are whopperheads, but he still loves them.
Travis thinks that Sean has a whopperhead, because it's big and square.
Travis thinks that Sean has a whopperhead, because it's big and square.
מאת O'Reilly 11 ביולי, 2006
Trending Now
Can you define these popular missing words?
1. Someone acting in a manner that represents a whopper 2. When you eat a Whopper from Burger King, you are being a Whopper Head 3. Just being a plain ol goofball.
"Don't order the chicken sandwich, Be a Whopper-Head" or "I cant believe you wrecked your car you Whopper-Head"
מאת Chronic2001 3 בדצמבר, 2008
someone that is acting silly or dorky
When you do that dance, you look like such a whopperhead
מאת j1969 21 בספטמבר, 2009
"everytime I take a hit I eat a bite of my whopper"
מאת The jizzmonkey at the end of the tunnel 2 באוקטובר, 2004


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- Clinical
- General
- Sport Psychology
- Industrial-Organizational
Program Goals
- Demonstrate mastery of knowledge and skills in psychology;
- Demonstrate the critical thinking, decision-making and general application skills necessary for ethical and competent performance in human services occupations;
- Conduct and integrate traditional and developing methods of research and scholarship and apply these methods to practical problems in human endeavors; and
- Communicate clearly and succinctly in professional writing, presentation and scholarship.
Curriculum Requirements
- Robert Morris University Core - 39 credits
These are the traditional liberal arts requirements of the University. Studies in humanities, communications skills, and social, behavioral, natural and quantitative sciences are included.
- Psychology Core - 36 credits
This component includes advanced course work in psychology. Studies in social psychology, child psychology, human growth and development, industrial psychology, abnormal psychology, physiological psychology, cognitive psychology, behavioral research and statistics are included, plus a two-semester practicum.
- Required Minor - 18 credits
This component requires students to complete an approved minor by taking a minimum of six courses in an area of interest.
- Open Electives - 27 credits
This component permits students to select courses from other University programs to personalize the program of study.
Click here to see a recommended course of study for the program.
Students complete an internship at sites such as: Allegheny County Medical Examiner's Office, Allegheny General Hospital Department of Psychiatry, Chartiers Mental Health and Mental Retardation Center, Early Learning Center, Family Behavioral Resources, Gateway Rehabilitation, Home Instead Senior Care, Pressley Ridge, RMU Athletic Department, Watson Institute.
The Psychology Advisory Board consists of local professionals who provide advice and input on the program and the internship component.


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Expanding Use of NGS Has Largely...
Wall Street Decline Takes Its Toll...
As Immunotherapy Revolutionizes the Cancer Treatment...
NSCLC is "Major Battlefield" as Merck,...
The use of oligonucleotides as therapeutic agents is of great interest, particularly when it comes to siRNA and antisense oligonucleotides. However, the difficulty of targeting the right organ and achieving efficient delivery to the cell remains a problem. There is much research activity directed at addressing these issues, employing solutions such as modification of the oligonucleotide backbone or the use of a delivery agent attached to the oligonucleotide.
In the case of the latter, various molecules including lipids, cationic lipids, cell-penetrating peptides and cell-targeting ligands have been reported to improve cell delivery by virtue of their hydrophobicity. This assists in their interaction with the hydrophobic environment of the cell membrane lipid bi-layer.
While these approaches have all been shown to enhance delivery, and in some cases even improve cell targeting, there are often associated challenges. Principally, even with successful delivery, there is still a need to release the oligonucleotide from the delivery agent once it has entered the cell to ensure efficient antisense activity.
One method of achieving this is the use of a cleavable disulphide bridge between the delivery agent and the oligonucleotide. However, when used in vivo there is the potential for toxic effects, since the delivery agents are frequently not recognized by the cells.
The use of vitamins as delivery agents has been shown to have potential in resolving this issue. Although vitamins such as tocopherol are not produced by the target cells, they are recognized by them, thus avoiding the issues of toxicity. They are also thought to be internalized by cells only after interaction with a binding protein, opening up the potential to target specific cell types using the technique.
Oligonucleotides modified with tocopherol via a disulphide-bridge are excellent candidates for the efficient delivery of factors such as therapeutic drugs directly into living cells. To make it easy to synthesize such custom oligonucleotides, Link Technologies has developed a range of tocopherol-based reagents. These simplify the incorporation of tocopherol into the 5´-end of an oligonucleotide and can be easily used with other modifications.
Although the direct incorporation of tocopherol into a therapeutic oligonucleotide should efficiently mediate its delivery into a cell, its hydrophobic nature might inhibit the final function of the oligonucleotide once inside the cell. To this end, we synthesized oligonucleotides conjugated to tocopherol via a cleavable disulphide-bridge.
After delivery to the cell, the tocopherol could be easily removed from the oligonucleotide by breaking the disulphide bridge, leaving the oligonucleotide free to carry out its therapeutic function. To demonstrate the feasibility of this approach, we constructed a cell delivery model system in which a hydrophobic cartridge takes the place of the cell membrane.
Oligonucleotides were synthesized using standard phosphoramidite chemistry, introducing a disulphide bridge linker followed by tocopherol incorporation at the 5´ end. The resulting crude oligonucleotide has the general structure tocopherol-S-S-oligo. After synthesis, the oligonucleotide was cleaved and deprotected, then immediately loaded onto a standard purification cartridge (Figure 1).
The purification method mimics what we would expect to happen in cell delivery, in that the modified oligonucleotide can be "delivered" to the cartridge and then the active molecule released via cleavage of the S-S bond, retaining the delivery agent (tocopherol) on the cartridge.
Using thiol-modified oligonucleotides conjugated to tocopherol as an example, we have generated data indicating that tocopherol successfully mediates attachment of the conjugate to the cartridge, and that the oligonucleotide can be released at a high level of purity following cleavage of the disulphide bridge.
This data indicates that the oligonucleotide can be easily released from the tocopherol delivery agent with sufficient efficiency and purity to accurately carry out its therapeutic function.
Catherine McKeen, Ph.D. ([email protected]), is a product manager at Link Technologies. Web: www.linktech.co.uk.
Catherine McKeen, Ph.D. ([email protected]), is a product manager at Link Technologies. Web: www.linktech.co.uk.
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Novum Pharma recently raised the price of an acne cream by over 3,900% in less than a year-and-a-half and Mylan increased price of EpiPen from $100 to $608 . Do you think pharmaceutical companies need to be subjected to price controls?
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- Summit Brings Together Global Myeloma Researchers, Clinicians, and Advocates
- IMWG Conference Series Live Stream Scheduled for June 11th
North Hollywood, CA, June 6, 2014 – The International Myeloma Foundation (IMF) – improving the quality of life of myeloma patients while working toward prevention and a cure – will host the 5th Annual International Myeloma Working Group (IMWG) Summit June 9 - 11 in Milan, Italy. The Summit is held each year prior to the annual Congress of the European Hematology Association (EHA). The goal of the IMWG Summit, which will be attended by a record 87 IMWG members, is to incorporate recent myeloma research advances into treatment for patients.
"The IMWG Summit provides a unique opportunity for the leading myeloma specialists from around the world to gather together to discuss and evaluate the newest research, including studies we are conducting collaboratively under the IMWG," said Dr. Brian G.M. Durie, IMF Chairman and Co-Founder and IMWG Chairman. "The Summit provides the opportunity for us to evaluate the latest developments in myeloma research and treatment, and come to a consensus on how best to incorporate these advances into recommendations for improving treatment for myeloma patients globally."
New myeloma research is front and center at this year's IMWG Summit. This includes the introduction of a new ultrasensitive myeloma testing method developed in conjunction with the IMF's signature Black Swan Research Initiative®. Susie Novis, IMF President and Co-Founder, noted, "the introduction of this exciting flow cytometry technique into clinical trials will move the Black Swan project significantly forward in its goal of finding a pathway to the cure for myeloma."
The IMF will share developments from the IMWG Summit directly with patients on June 11th with the popular IMWG Conference Series, "Making Sense of Treatment," which will be live streamed from Milan. The live webcast will feature international myeloma experts Drs. Brian Durie, Ola Landgren, Joseph Mikhael, Antonio Palumbo, and Stephen Russell, who will debate and discuss the latest advances in myeloma research and treatment. The webcast will stream live at 12 pm ET on June 11th. The live and archived webcast can be found HERE.
Other highlights expected during the IMWG Summit include:
The IMF founded the IMWG, which includes 160 leading myeloma researchers from around the world, in 2001 to evaluate and conduct collaborative research, provide consensus opinions, and improve myeloma treatment options and diagnostic systems.
The Black Swan Research Initiative receives support from private donors, as well as a multi-year sponsorship from Onyx Pharmaceuticals, Inc., an Amgen subsidiary.
Celebrating its 23rd anniversary, the International Myeloma Foundation reaches more than 350,000 members in 120 countries worldwide. A 501 (c) 3 non-profit organization dedicated to improving the quality of life of myeloma patients and their families, the IMF focuses on four key areas: research, education, support, and advocacy. To date, the IMF has conducted more than 250 educational seminars worldwide, maintains a world-renowned hotline, and established the International Myeloma Working Group (IMWG), a collaborative research initiative focused on improving myeloma treatment options for patients. The IMF can be reached at (800) 452-CURE (2873). The global website is www.myeloma.org. Follow the IMF on Twitter @IMFmyeloma.
Debra Gendel
(310) 710-1903


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Learning from history: Manure's value is ageless
Learning from history: Manure's value is ageless
Let's continue the synergism of crops and livestock.
By Natalie Rector
The author is the senior nutrient management educator for Michigan State University Extension. For more information, visit www.animalagteam.msu.edu
Utilizing manure as a fertilizer to its full potential is a concept that has made cents and sense for well over 100 years.
The statement, "One of the important functions of farm manure is to convey back to the soil, in a readily available form, the majority of the plant food removed from it by crops." was taken from Farm Manures by A. H. Snyder. It was the first Extension bulletin published at Iowa State College (now ISU) in October 1907.
It is interesting that the first Extension bulletin in Iowa focused on manure. Even more interesting is how much researchers knew about manure 103 years ago. It makes you wonder how much further along we'd be with manure management today if we would have held onto the basic premise of the synergism of crops and livestock.
The bulletin begins with a concern that a quarter of the crop in Iowa was leaving the farm and, therefore, it would never be recycled (as fodder, bedding, or manure) back to the soil. He feared this would result in lower soil quality. Snyder made a strong social statement when he wrote, "If anyone has the least doubt as to the necessity of adopting proper methods in the treatment of his soil, he need only consider the history of the older states a little farther east. He will see that the march of commercial fertilizers has advanced westward until they now have a strong foothold in Illinois . . . and are knocking at the door of our own state. It is high time for the land owner of Iowa to decide whether he will adopt such methods of farming as will permit returning to the soil a large percentage of the plant food removed by crops, or whether he, like his brother farther east, will rob his soil by wasteful and haphazard methods, until it is no longer a question of maintaining, but rather a question of acquiring or building up fertility."
Most of us in agriculture probably wouldn't be quite so hard on commercial fertilizer, and we certainly acknowledge the advances in feeding the world it has provided, but is there some merit in balancing soil fertility with something as natural as manure?
Still need the land base
The areas in the United States that have the largest issues with manure management are those where the land base to raise the crops to feed the animals has vanished. States that have the land base struggle with social and environmental pressures to land apply manure while feeling pressured to use expensive technical fixes that keep it out of sight and smelling distance. Regardless of Snyder's social and political motives, he supports his ideas with data on profitable handling, storing, and manure nutrient utilization. The ol' pocketbook is the cord that still rings true to the heart of everyone, including farmers.
The bulletin never uses the word sustainability, but a combination of livestock and cropping systems was valued to achieve a nutrient balance and a profitable family farm. Although farms have specialized like every other business, sustainability can be found in a neighborhood where crop and livestock producers share resources.
The value of manure for its nutrient and soil-enhancing qualities was front and center of farmers' minds in 1907. Though the bulletin does not talk about environment protection, it strongly states that losing nutrients should be avoided at all costs.
"When manure is allowed to accumulate, it is always important that proper precautions be taken to prevent loss by leaching and fermentation. It should never be piled under the eaves of a building, nor on a slope where surface washing will be great. When possible, it should either be sheltered from rain by some sort of shed or placed in a water-tight pit, so that the water which falls upon it cannot leach away the most valuable portion of the manure."
The bulletin goes on to quote research that shows the amount of nutrients, dollar value of those nutrients, and yield impacts that can be lost from crop production by "haphazard methods of collection, preservation, and utilization of manure." Still timely advice 103 years later.
Not one answer
There is no silver bullet for areas that just do not have the land base to agronomically apply manure, but there are options. For farmers who live in neighborhoods where there is the land base — whether they own the land or not — seeking partnerships will ensure sustainability. If you rely on purchased fertilizer, would you consider working with, or even encouraging a new livestock farm in your neighborhood for long-term sustainability?
For livestock producers, manure is an expensive issue. Maximizing manure nutrients by returning them to the land is financially imperative.
For those with a small land base, your soil tests may show that phosphorus fertilizer is not necessary. Are you still buying it? Are you telling yourself that it is needed because you plant early and the soil is cold and/or you no-till? Can you really afford purchasing phosphorus starter fertilizers agronomically and financially? Or are you waiting for social and regulatory pressure to persuade you?
Snyder continued, "The results of numerous experiments show that one of the most important roles which manure plays is that of rendering available the native plant food of the soil. While it has been demonstrated beyond reasonable doubt that this is true, it is, nevertheless, not as thoroughly understood how it is accomplished." Nor is it thoroughly understood today.
Link: To find the full bulletin, visit http://bit.ly/ISUManure.
Get the most from manure nutrients
Want to make sure you're getting the biggest bang for your manure buck? Follow these simple tips:
• Take soil tests, and follow the recommendation they unveil.
• When fertilizer is not called for, research has proven time and again it will not consistently or profitably add to yields.
• Trust in manure nutrients, and learn how to maximize your system to recycle and retain the most nitrogen.
• Find ways to haul manure to fields farther away that do need the P and K, and reduce fertilizer accordingly.
• Swap land with your neighbor when their land is closer than your own to haul manure.
• Be sure soil pH and compaction are corrected to allow all nutrients to work for the crop.
Above all, credit the manure nutrients first, using fertilizer only when there is a proven shortage in the soil. Be sure your agronomist provides you with a copy of the soil test results, not just the recommendations. It's your land, your money, and your future.


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The Altoona Symphony Orchestra is rounding out its season with musical mastery.
The ASO's 2012-13 season will conclude with the concert entitled "Brahms 4th," after the last symphony of German composer Johannes Brahms.
"This concert features two key composers in the history of German Romanticism: Robert Schumann and Johannes Brahms," ASO Maestra Teresa Cheung said in an email. "The friendship of the two composers has been well documented. As a composer and a music critic, Schumann wrote [openly] about his beliefs in Brahms' music, so in many ways, Brahms owed his success to the unwavering support of Schumann, a composer, mentor and close friend 23 years his senior."
Article Photos
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The Altoona Symphony Orchestra, under the direction of Maestra Teresa Cheung, will explore German Romanticism in its final concert of the season, to be held Saturday at the Mishler Theatre in Altoona.
Romanian-born cellist and conductor Ovidiu Marinescu has performed throughout the world.
The concert includes an overture from Schumann's only opera, "Genoveva," and his only concerto for the cello, Cheung said. Schumann wrote the compositions just before his death in 1856.
"Brahms' No. 4 in E minor," his last symphony, will compose the second half of the concert, Cheung said.
"The work was not a nod to the future as many of the composers at the time would favor; rather, it was Brahms' salute to the grand tradition of German music by incorporating the theme of Johann Sebastian Bach's 'Cantata 150' in the last movement of this great work," she said. "This concert is going to allow our audience to discover how simple ideas [can] be crafted and turned into something extraordinary in the hand of a master."
Fact Box
If you go
What: Altoona Symphony Orchestra's "Brahms 4th"
When: 7:30 p.m. Saturday
Where: Mishler Theatre, Altoona
Tickets: $33, adults; $13, students. Available online at www.mishlertheatre.org, or at the box office, 1208 12th Avenue,?Altoona
Details: The concert is in honor of ASO long-time supporters Allen and Marilyn Goldberg
The concert will feature cellist and conductor Ovidiu Marinescu playing the cello concerto by Schumann.
"I feel a deep, personal connection to this work, perhaps because I've had the opportunity to perform it many times, and each time it seems to reveal more and more of its messages hidden behind the notes," he said in an email. "Last month, when I played it most recently, I had a new revelation: I felt that the slow, lyrical second movement represents the innocent soul of a 5-year old, the inner child in each of us."
Under Schumann's design, the three movements of the concerto should have no pauses between playing them, Marinescu said.
"A unique aspect of this concerto is a certain duality of emotion and character. The concerto has a sweet melancholy contrasted with a more heroic side. Perhaps this is a manifestation of [Schumann's] fictional characters Florestan and Eusebius he created in 1831 as a reaction to Jean Paul Richter's use of twins to express the duality of a man's personality," he said.
"The two characters represented the dual personality he imagined for himself. Florestan represented Schumann's masculine side, and Eusebius his feminine characteristics. Another possible explanation of this duality is a possible artistic and subconscious manifestation of his life-long struggle with mental illness, possibly bipolar disorder or schizophrenia, first occurring in 1833 as a severe melancholic depressive episode alternating with phases of exaltation."
Among many accomplishments as a musician and a conductor, Marinescu, who is director of the West Chester University Symphony, has played throughout the world including Carnegie Hall, according to his resume. Marinescu, who is from Romania, is also a regular guest conductor at the Russian Philharmonic Orchestra.
"Ovidiu is one of the most amazing and versatile artists I have ever met. Besides being a wonderful cellist, he is also a fantastic teacher and conductor," Cheung said. "What is so unique about him is the value that [he] puts on music and how he influences so many young lives with not just the cello, but as an interpreter of symphonic music because of his conducting. I have worked with many solo artists, but performing with someone like Ovidiu is something extraordinary because of his in-depth knowledge of the entire score."
This is Marinescu's first performance with the ASO. He cannot wait, he said.
"I had the pleasure of hearing the orchestra in a wonderful rehearsal last September, where Maestra Cheung inspired the musicians in Ravel," he said. "I hope the audience will be able to use the performance as a vehicle for introspection, inspiration and artistic energy."
Mirror Staff Writer Amanda Gabeletto is at 949-7030.


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LC-MS Gains Favor Due To the...
Process Development Jobs Still the Most...
Wall Street Decline Takes Its Toll...
Adapting to Evolving Business Environments Is...
Agilent is buying Danish cancer in vitro diagnostics firm Dako for $2.2 billion in cash on a debt-free basis. Agilent says the acquisition is the largest in its history, and will complement its existing life science business and boost its presence in the clinical diagnostics market. "In the rapidly growing diagnostics market, Dako's products and capabilities are a strategic complement to Agilent's existing offerings," comments Bill Sullivan, Agilent president and CEO. "Dako is one of the world's leading providers of cancer diagnostics tools, and together we will be able to develop a wider range of products."
Tissue-based diagnostics firm Dako provides antibodies, reagents, instruments, and software to the clinical pathology market worldwide, and also collaborates with the pharma industry on the development of companion diagnostics. The firm reported annual revenues of some $240 million in 2010, and employs over 1,000 people worldwide.
Dako separately today announced a license, manufacturing, and distribution agreement with the Van Andel Research Institute for the development of cancer diagnostics utilizing the MET4 antibody. Under terms of the deal Dako will develop and commercialize MET4-based diagnostic tools worldwide. The firm also retains the right to develop MET4-based companion diagnostic assays in collaboration with pharmaceutical companies.
Today's license agreement between Dako and the Van Andel institute represents the latest in a string of recent collaboration agreements. Since December Dako has announced two new cancer drug companion diagnostic development agreements with Amgen, and two collaborations with Genentech. The Genentech deals are focused on submitting regulatory filings to FDA for approval of Dako's HercepTest™ and HER2 FISH pharmDx™ tests as companion diagnostics for identifying breast cancer patients who may be eligible for treatment with either pertuzumab or trastuzumab emtansine.
FDA approved Dako's HER2 CISH pharmDx™ diagnostic test in December as an aid to identifying breast cancer patients who may be suitable for Herceptin® therapy.
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While Zika continues to garner a lot of news coverage, a Mayo Clinic survey reveals that Americans believe the country's most significant healthcare challenge is cancer. Compared to other diseases, does the possibility of developing cancer worry you the most?
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Snowy owls seen in Erie area
Snowy owls, at least five of them, have been spotted recently in the Erie area, mostly on Presque Isle State Park.
"They're not from around here so it's exciting," birder and photographer Shawn Collins, of Meadville, said.
He saw three of the birds, which breed in the Arctic tundra, Friday at the peninsula.
See Wednesday's Erie Times-News and GoErie.com for more coverage.


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Tiffin reports
A 1600-block West Market Street woman reported Wednesday afternoon she found a revolver near a fence in her yard.
A first-block Hall Street caller requested to speak to an officer Wednesday night about a stolen laptop.
A caller reported early Thursday morning an intoxicated man was passed out in a car in the first block of Main Street. The intoxicated man left on foot. Police were unable to locate him.
A break-in was reported Thursday morning at Klay Tire & Oil, 230 E. Market St. Catalytic converters were taken off of three vehicles and change was taken from a cash drawer.
County reports
A caller from Mike's Auto Body, 1560 W. CR 54, reported early Thursday morning the front door appeared to have been broken open.
A break-in was reported Thursday morning at Bish Construction, 1650 TR 1186.
Fostoria reports
A caller in the area of Palmer Street and Springville Avenue reported Wednesday evening several kids were playing "chicken" with vehicles. Police were unable to locate the kids.
A 100-block West Crocker Street man reported Wednesday evening he received a scam phone call.
An 800-block Leonard Street woman reported Wednesday night her boyfriend moved her car and wouldn't move it back.
Tiffin fire
Firefighters were called out Wednesday evening to 870 W. Market St. and 187 Jackson St. for fire alarms. Both calls were false alarms.
County fire
Sycamore firefighters were called to South CR 19 Wednesday evening for a chimney fire. McCutchenville firefighters were put on stand-by.
Bettsville firefighters were called to South SR 269 Wednesday evening for the report of smoke.
Fostoria Municipal Court
Jessica J. Welly, 701 Hart Ave., failure to control, $187; Cody W. Colebank, 309 1/2 S. Main St., Apt. 2, assured clear distance, $229; Melissa D. Knisely, 215 Bricker St., assured clear distance, $125; James C. Horn, Cincinnati, tag/sticker violation, $125; Clyde S. Malagon, Helena, speed, $141; Dean C. Mosier, Fostoria, speed, $105; Paul E. Danner, 345 Perry St., stop sign violation, $125; Brittany L. Clark, Fostoria, speed, $177; John H. Escher, 461 W. Lytle St., Lot 120, traffic control light violation, $125; Matthew T. Leddy, Bowling Green, speed, $117.
Aaron C. Wilson, 1224 Sandusky St., Apt. 45F, tag/sticker violation, $125; Richard A. Zeis, Sr., Tiffin, expired tags, $203; Joseph J. Hunt, Risingsun, speed, $120; Tammy S. Bates, Findlay, speed, $111; Todd W. Short, Archbold, failure to control, $125; Joshua M. Schlarman, Celina, no tail light violation, $125; Laura E. Bordeau, Columbus, assured clear distance, $125; Leane M. Schock, Tiffin, speed, $219; Paige K. Huber, Pataskala, speed, $138; Jeanne M. Wahrer, New Riegel, speed, $114.
Leah N. Hunt, 654 Maple St., disorderly conduct, $115; Carrie S. McDermott, Bexley, speed, $126; Alfred M. Zeigler, III, 618 E. Jackson, Apt. 3, speed, $130; Genevieve M. Lockmiller, North Baltimore, speed, $135; Eric N. Mullholand, Arcadia, seat belt violation, $142; Savath Sengmany, Eastlake, tag/sticker violation, $125; Jeffrey A. Watkins, Upper Sandusky, seat belt violation, $75; Kenneth U. Arbogast, New Riegel, assured clear distance, $125; Takis M. Robinson, 632 Wall St., lane/line violation, $125; Dustin B. Soals, 869 Columbus Ave., speed, $120.


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Politics has been marred by sex scandals throughout American history. Take a look at some of the steamiest.
History's steamiest political sex scandalsUPDATED 2:23 PM EDT Oct 31, 2014
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