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Wall Street Decline Takes Its Toll...
As Immunotherapy Revolutionizes the Cancer Treatment...
Developers of DTC Genetic Testing Are...
Expanding Use of NGS Has Largely...
Gene silencing by RNA interference has become a key tool in research and drug discovery since its discovery by Andrew Fire and Craig Mello. RNAi allows for the sequence-specific knockdown of target genes with a subsequent phenotypic analysis, representing a straightforward method for ascribing functions to genes.
The advantages of RNAi include the high efficiency of the gene knockdown, the ability to easily target the gene of interest, as well as stable and long-term silencing by expressing shRNAs. This makes for a powerful tool that has been successfully applied to answer many questions in cell biology. There are also risks, however, when using RNAi that need to be considered carefully.
Researchers have demonstrated that RNAi sequences do not only bind to one target. These changes in the gene expression pattern of the cell and potentially of the phenotype give rise to an off-target signature. The unidentified effects of such a signature bear a high risk to create false-positive outcomes that can bring a complete project into jeopardy. Furthermore, even most up to date algorithm-based sequence designs show a knockdown efficiency that is generally at 80% or less.
The effects of such specific but low knockdown can be masked by the off-target signature with phenotypic changes being undetectable. These issues can make RNAi unpredictable, slow, and risky, in particular in drug discovery, where speed and reliability of results are crucial factors. Sirion Biotech has developed technologies that help overcome the drawbacks of RNA interference.
One particular challenge with shRNAs is finding sequences that are highly effective with a validated efficiency of over 80%. Sirion has developed RNAiONE™ for fast and reliable identification of the most efficient sequence for a specific gene.
RNAiONE has been used in the silencing of murine Hsp90b, a difficult target due to its abundance. Ten validation vectors containing different bioinformatically evaluated shRNA sequences targeting Hsp90b were transfected in an optimized cell system expressing the GFP-tagged Hsp90b protein from the same vector.
Initial microscopic analysis provided first evidence of shRNA efficiency based on the GFP readout. Validation of the knockdown was then carried out by measuring the mRNA level with real-time PCR and confirmed by Western blot analysis. The results show that the efficiency can vary tremendously for different target sequences with a knockdown between 0 and over 90% (Figure 1), demonstrating the need for an appropriate validation protocol.
The data also illustrates that a highly effective sequence could be successfully identified, despite the high abundance of Hsp90b in the cell. Furthermore, results with RNAiONE can be applied reliably to Sirion's different viral vector platforms providing immediate access to subsequent gene analysis and assay cell model generation. The overall effectiveness of RNAiONE allows Sirion to identify shRNA for most of the genes and to issue warranties to its customer labs.
A prerequisite to successfully conduct meaningful shRNA experiments, however, is not only a high and validated knockdown, but also the use of appropriate controls. VariCHECK™ serves as such a perfect built-in control for RNAi experiments which allows confirmation of on-target phenotypes. This system enables the depletion of an endogenous targetX, while simultaneously overexpressing the ectopic undegradable form of targetX, which then rescues a specific phenotype (Figure 2).
The basis of VariCHECK is Sirion's All-In ONE vector inducible lentiviral system based on Clontech's latest 3G technology. The constitutive TET-dependent transactivator is simultaneously expressed together with the TET-inducible gene of interest or the validated shRNA. Cells are transduced with a first vector that contains the inducible shRNA targeting the wild-type gene of interest (GOI) and a second vector with the inducible ectopic undegradable form of the GOI.
Without Doxycycline (Dox), neither shRNA nor ectopic GOI expression takes place and the endogenous wild-type protein is present in the cell. In contrast, with Dox, wild-type transcripts are degraded by the expressed shRNA, while the ectopic protein is present.
The validity of VariCHECK has been successfully demonstrated for the functional analysis of an oncology target (GOIx). A stable cell line was generated that expressed the TET-inducible shRNA against GOIx, previously validated with RNAiONE. Gene knockdown was quantified, and the best performer cell clone with near quantitative knockdown was then transduced with lentiviral vector 2 that encoded the inducible ectopic undegradable GOIx.
Expression of GOIx in the absence and in the presence of Dox was quantified by real-time PCR and Western blot analysis, respectively, confirming an almost quantitative switch from wild type to an ectopically expressed form of GOIx on mRNA as well as protein level. Moreover, the inhibitory effect of GOIx knockdown on cell proliferation was fully rescued by ectopic expression of the undegradable GOIx, demonstrating the reduced proliferation as a real on-target phenotype (Figures 3A–C).
Beside its function as RNAi built-in control, VariCHECK can be also applied to reversibly switch expression between the wild-type protein and a defined mutant version in a single cell line. This approach can be used to undertake functional analysis of proteins and their mutations in a fast and equally reliable manner, which is of particular interest for the analysis of escape mutants from cancer drugs.
Since its discovery in 1998, RNAi silencing has become the method of choice for phenotype studies; however, there are some risks that need to be considered including an insufficient knockdown and potential off-target effects. This can be highly critical in drug discovery and development, and add another question mark in compound and target validation, a process that is already lengthy and risky. Technologies such as RNAiONE and VariCHECK make the use of RNAi for scientists faster, more predictive and reliable in particular in drug discovery.
Kathrin Schmitt, Ph.D. ([email protected]), is director, key accounts of Sirion Biotech.
Kathrin Schmitt, Ph.D. ([email protected]), is director, key accounts of Sirion Biotech.
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Colourful Links on Sunday
It is grey here but this Autumn we are surrounded by the most vivid and fantastic colours so I thought the theme of the day is colours, I am going t share some photos of mine from my own immediate surroundings and some links to colourful sites and blogs. Enjoy the
– Quilling, an old Renaissance art still alive!
– You can easily do this to bring more colour to your kitchen – so simple but what an effect!
– I cannot but admire and like Bento box artists because they do what they do. With colour.
– These photos might be in b&w but they are truly colourful in the subject matter.
– And light is colour, here it is more light than colour but just see what it can do!

subset: commoncrawl


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Christmas Wine Glasses & Christmas Glassware
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'Allies' | Viscose-Blend Tuxedo Blazer by HUGO
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Just Cause 2 for Xbox 360On: Xbox 360, PS3, PC
With more stunts, vehicles and weapons than ever before and an incredible overhauled grappling hook, Rico returns to action in Panau.
Stuck on Just Cause 2? We've got you covered!
Don't panic! Our cheats will see you through.
Just Cause movie easier to greenlight if Hitman movie is a success
Thankfully the decision will be based on other factors too.
Just Cause 3 to be announced next week?
Avalanche founder thanks fans for their patience following leak, asks them to "enjoy the silence for a couple of days".
Ex-Virtuos employee says he worked on Just Cause 3
But we're still waiting on an official announcement.
Just Cause dev has 'several surprises' in store for 2015
Just Cause 3 reveal due next year? Avalanche teases future plans as it expands studio.
Cause and effect: The full story behind Just Cause 2's multiplayer mod
Just Cause 2 didn't ship with a multiplayer mode at launch. But that didn't stop six members of the community making one.
Mad Max coming April 2014, Just Cause 3 in summer 2015?
Studio head's online CV outs release dates for upcoming open-worlders.
Xbox One cloud is 'one area console has advantage over PS4', says Avalanche tech lead
But believes Sony could implement a similar feature in PS4 if Microsoft is successful.
Avalanche boss 'understands' Xbox One disappointment
But thinks Microsoft's lack of focus on games is fan's biggest bugbear, rather than the console itself.
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Tuesday, March 12, 2013
What: First United Methodist – Isle of Palms 23rd annual Tea Room
When: Wednesday, March 13-Saturday, March 16, 11:30 a.m.-2 p.m.
Where: First United Methodist Church, 21st Avenue and Palm Blvd, Isle of Palms
Details: All items homemade, prepared fresh each day. Additionally the Crafty Ladies boutique will be open featuring many unusual items for the home as well as gifts. Reservations for groups are encouraged. Takeout orders also accepted. The church office phone number is 886-6610, FAX number is 886-3760 and the website is www.iopmethodist.com.
WHAT: Charleston Chi Omega Alumnae Association Meeting
WHEN: Thursday, March 14, 6-8 p.m.
WHERE: Holiday Inn Charleston Riverview
DETAILS: All Chi Omega Alumni are invited for the first membership meeting of 2013. Welcome new faces, as well as old friends, for an evening of good food and great conversation. Admission is $10 for members or $15 for non-members. Visit www.facebook.com/events/220380881433265/ for more details.
WHAT: Breakthrough Revival
WHEN: Thursday, March 14; Friday, March 15 at 7 p.m.; and Saturday, March 16 at 10 a.m.
WHERE: Holiday Inn, 250 Johnnie Dodds Blvd.
DETAILS: The event is the Breakthrough Revival with Apostle Steven Brooks. Brooks is a best-selling author and is a pastor in North Carolina. He states that he moves mightily in signs, wonders and miracles, so come expecting a miracle. There is no charge for admission but seating is limited. Register at email@example.com. For more information, contact Dennis at 843-343-3559.
What: VFW Post 10624 and Ladies Auxiliary Corned Beef and Cabbage Dinner
When: Friday, March 15, 6-8 p.m.
Where: VFW Post 10624, 1411 Stuart Engal's Blvd
Details: The cost is $10 per person, and Ruenens will also be available for $7. For more info call 843-884-0062.
What: East Cooper Outboard Motor Club Ladies' Auxiliary 12th annual spaghetti dinner
When: Friday, March 15, 5-8 p.m.
Where: Gold Bug Island
Details: All dinners are $8. Homemade desserts will be available for sale and there will be a silent auction with lots of goodies. Tickets in advance call (843) 343-0855 or maybe purchased night of event. Eat in or carry out.
What: The USCG Auxiliary, Flotilla 12-6, will offer a Boating Safety Course.
When: Saturday, March 16
Where: Daniel Island Library, 9 a.m.-5 p.m.
Details: This one day class will cover subjects like: underway preparations, legal requirements, navigation rules/charts, boating emergencies, operating your boat safely, federal/state regulations, and enjoying water sports. A fee of $30 per individual or $35 per family (up to six) applies to cover materials. This course is certified by the USCG Auxiliary and the SCDNR. To register for the course contact Chris Gallapis at 843-729-9564, or email firstname.lastname@example.com.
WHAT: "How to Best Use Emergency Medical Services" presented by Don Lundy, Director of Emergency Medical Services (EMS) for Charleston County and President of the National Association of Emergency Medical Technicians.
WHEN: Saturday, March 16, 10:30 a.m.-noon.
WHERE: East Cooper Medical Center, 2000 Hospital Drive
Details: This free talk will be presented by Don Lundy, director of Emergency Medical Services (EMS) for Charleston County and president of the National Association of Emergency Medical Technicians. Meetings are held on the third Saturday of each month from 10:30 a.m. to 12:30 p.m. firstname.lastname@example.com or call 843-388-1683.
What: 2013 Carolina Yard Gardening School
When: Saturday, March 16
Where: Exchange Park in Ladson
Details: Join Clemson Extension, the Tri-county Master Gardeners and other partners and sponsors for a fun-filled, educational event for every gardener. Participants will enjoy two lectures, two workshops, a delicious lunch, door prizes and free compost. Registration is $75. Contact the Urban Horticulture Extension Agent firstname.lastname@example.com or calling 843-722-5940, ext 122.
What: Author Cindy L. Hanson book signing event
When: Saturday, March 16, 11 a.m. to 4 p.m.
Where: Atlanta Bread Company, 1712 Towne Centre Way, Mount Pleasant
Details: Dr. Hanson's books, "A Mighty Eagle" and "Be Loved Eagle," from her book series, "A Beloved Bird," will be available for purchase, along with finger puppets that accompany the book series. Author proceeds are donated to charity.
What: James Island/Folly Beach Democrats oyster roast
When: Sunday, March 17, 2-5 p.m.
Where: Bowen's Island
Details: Join James Island/Folly Beach Democrats for the group's second annual oyster roast. Tickets are $20 per person. There will be BBQ and Vegan chili for non-oyster eaters. Beer, wine, and treats will be for sale. For tickets, contact Penny Cromwell, project chair, at 843-607-6298 or email@example.com or Nancy Moore, JIFB secretary at 843-408-5774 or firstname.lastname@example.com.
What: Fundraiser for Crown Leadership Academy
When: Sunday, March 17, 3-6 p.m.
Where: Awendaw Green
Details: Celebrate St. Patrick's Day with Crown Leadership Academy. Live music will be performed by Volcanoes in the Kitchen. There will be BBQ and all the fixins' provided by Sticky Fingers from to 4 to 5 p.m. Beer and wine will be for sale and there will be a bake sale fundraiser, silent auction and face painting. Please RSVP- to Laurie Turner at 843-200-9912 or www.crownleadershipacademy.org. Adult tickets are $15 in advance and $20 at the door. Children 5 and under are free. Bring a chair or blanket. All "Crown the Kids" donations are tax deductible except the fees.
What: Charleston Music Club free Musical Program
When: Monday, March 18, 7:30 p.m.
Where: Chapel at Franke at Seaside, 1885 Rifle Range Rd.
Details: The Charleston Music Club will present the College of Charleston Musical Theater in a free musical program in the chapel at Franke at Seaside followed by refreshments. Call 442-4835 or visit charlestonmusicclub.org.
What: The Shepherd's Center of East Cooper weekly meeting
When: Tuesday, March 19, 10 a.m.
Where: The Fellowship Hall of Mount Pleasant Presbyterian Church
Details: The first program at 10 a.m. will be a talk by Barbara Bond on "The Ronald McDonald House." The second program at 11:15 a.m. will be a talk by Sheriff Al Cannon on "Law Enforcement in Charleston County."
What: East Cooper Newcomers Club monthly luncheon meeting
When: Wednesday, March 20, 10 a.m.
Where: Holiday Inn, Mount Pleasant; 250 Johnnie Dodds Blvd.
Details: The program will be by Doug Henderson, director of the Charleston County Public Library system who will be the featured speaker. Call Elaine no later than March 14 at 843-856-3467 or email at firstname.lastname@example.com.
What: Mount Pleasant Business Association monthly Member-Guest Luncheon
When: Thursday, March 21, 11:30 a.m.
Where: Mount Pleasant Holiday Inn Express, Johnnie Dodds Boulevard
Details: This month's featured sponsor will be Bill Machio, of Charleston Magazine. Anyone who does business in Mount Pleasant is encouraged to attend and explore all that the association has to offer. Guest fee for the luncheon is $18 at the door. Lunch is served at noon.
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Thursday, May 2, 2013
The South Carolina United Mount Pleasant '01 Independence girls U11 soccer team coached by Christi and TJ Rostin took first place in the 2013 Julian Brown Memorial Cup tournament in Charlotte, N.C. on April 20-21. The girls played in the most competitive U11 Girls Elite flight against some good teams and earned a first place finish in the finals against the SSA '01 Black Storm from Savannah, Ga., in a 3-2 finish. Team members include (top row, from left) Regan Jones, Meaghan McLoone, Marianella Roncancio-Gillum, Britton Blom, Brandi Reilly, (middle row, from left) Caroline Johnson, Alexis Demarco, Gabby Short, Hadley Martel, Macara King, Coach Christi Rostin and (front) goalkeeper Haley Lowell. (Not pictured is coach TJ Rostin.)
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A South African court granted bail today to Oscar Pistorius. Pistorius is accused of the premeditated murder of his girlfriend, model and lawyer Reeva Steenkamp. Although there were several arguments offered by Pistorius' attorneys urging bail, the most interesting, for me, was their assertion that the "Blade Runner" was too famous to pose a flight risk.
The decision by Magistrate Desmond Nair was cheered by Pistorius' family and supporters, although he, himself, showed no reaction. This is in contrast to his appearance at an earlier hearing this week wherein he broke down in almost uncontrollable tears.
The court set bail at 1 million rand ($113,000) and set the next hearing in this matter to June 4.
Pistorius' bail conditions are as follows:
1) Hand over firearms and passports
2) Avoid his home and all witnesses in the case
3) Report to a police station twice a week
4) No alcohol or illegal drug consumption.
Prosecutors said that on Valentine's Day, Pistorius, 26, fired four shots into a locked bathroom door, hitting Steenkamp, who was cowering on the other side. Steenkamp, 29, was hit in the head, hip and arm.
Pistorius' defense team argued the killing was a tragic accident and mistake. The athlete, they argued, had mistaken Steenkamp for an intruder.
They also said that this man is simply too well known worldwide to pose a flight risk. He deserved and needed to help his attorneys prepare for a case that is likely to be the first "trial of the century" for the 2000s.
"He can never go anywhere unnoticed," his lawyer Barry Roux told the court on Friday.
The Olympic and Paralympic star faces life in prison if convicted of premeditated murder.
Prosecutors, on the other hand, portrayed Pistorius as a cold-blooded killer.
"You cannot put yourself in the deceased's position. It must have been terrifying. It was not one shot. It was four shots," prosecutor Gerrie Nel said today.
In an affidavit, Pistorius said he had been "deeply in love" with Steenkamp. Roux asked what possible motive could Pistorius have for the killing.
Again, Pistorius claimed that he was acting in self-defense. He mistook Steenkamp for an intruder. He was unable to attach his artificial legs in time to meet the intruder head-on, his attorney said.
So, after pulling a 9-mm pistol from under his bed, he approached bathroom and fired into the shut and locked door which adjoined the bathroom. Ear witnesses say they heard a gunshots and screams coming from the athlete's home. The community is surrounded by 3-metre-high (more than 9 feet) stone walls and crowned with another electrified fence.
In perhaps the most bizarre twist yet in this case, the South African police removed their lead detective from this case just yesterday. It was revealed that he himself faced attempted murder charges in another case. He has been replaced by South Africa's top detective.
The "too famous to flee" argument posited by Pistorius' attorneys is, to say the least, novel. Although I am sure it has been used in American courts, I have never heard of it being successful. Apparently, the worship of "celebrity" in South Africa is even more overweening than it is here.
Just imagine. A super-famous accused person tells an American judge, "Where can I go, your honor, where no one would know me?" Can you see any American judge responding, "Okay, Mr./Ms. Celebrity, you are free to go. Just be back next week for your next hearing."?
I doubt it.
Meanwhile, Steenkamp gets no reprieve or bail from her death sentence.


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Sources: NASA, USA Today, CNN
quote: Oh come on, give me a break. Have you ever watched Mythbusters? Particularly the one where they go to the Apache Point Observatory in New Mexico and shoot a laser at the reflector panels that the lunar astronauts left up there?
quote: It is obvious I am not the only one that thinks the moon landing was BS
quote: especially after this Mars rover landing.
quote: Mars is too high in gravity...


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Special to the Sun-Gazette
Everyone has heard the old saying, "the gift that keeps on giving." Some spend their time wondering, "Is there really such a gift?" In time, the perfume bottle is empty; the food inevitably will perish and, while a gift to a charitable cause is a wonderful idea, it is difficult to ensure it stays local. Is there really a gift that continues to give throughout the year?
Signs of the holidays began cropping up weeks ago. Even before the turkey was on the table for Thanksgiving, people were beginning to wander store aisles in consideration of the perfect Christmas gift - and the stores were ready.
In just a few days, it will be Christmas. And in another week, Christmas 2012 will be another holiday to mark off the calendar. But it doesn't have to stop there. Lycoming County United Way believes in living united and giving united. That giving does not stop when Dec. 25 comes to an end. Last year, the money raised by LCUW helped make it possible to serve more than 46,000 individuals in Lycoming County. The needs of our neighbors continue to grow each year and with the support of the community, Lycoming County United Way strives to ensure that local agencies are able to provide the services that make life easier for those in need.
"The human service gifts we provide to those in need are accomplished via a detailed allocation process using a combination of community needs assessment surveys, panels of volunteers who review our program partner requests and outcomes and the combined knowledge we've all gained in our daily work with the human service community," explained Scott N. Lowery, LCUW executive director. "This process has significantly changed in the last four or five years as we have funded several new programs meeting emerging needs and discontinued funding of some other programs. We try and direct our funding to the most pressing needs."
In 2011, the Central Pennsylvania Food Bank reported that it served more than 8,000 individuals who were in need of food.
Jamie L. Caputo, branch director, said that the demand for food in the county has increased significantly.
"Across the board we've been experiencing 15 to 20 percent increases with our agencies," Caputo said. "No county is really an exception."
She added that about 1,800 households in Lycoming County are accessing food on a monthly basis.
"That is just from the major food pantries," Caputo said. "That doesn't include shelters and soup kitchens. When you throw in the soup kitchens and shelters, it's easy to say there are 2,500 households accessing food monthly. We've got agencies that are serving 3,000 or more meals a month. And the winter really moves people back into the pantries."
Caputo said she expects the need to increase over the next year. Based on the economic predictions, it looks even scarier, she added.
"If some of those predictions come true, it looks like we are heading back into a recession and with a recession comes more needs at the pantries, kitchens and shelters," Caputo said.
Oftentimes, food and housing needs go hand-in-hand. The American Rescue Workers reported that it served 598 individuals last year.
According to Col. Dawn Astin, the American Rescue Workers has seen a turnover with homelessness that appears to be getting worse.
"I think it continues to get worse," Astin said. "We had a 75-year-old homeless veteran. That's a real problem with homeless vets and I don't see that improving. In fact, I think that's getting worse."
These, along with the needs of many others throughout the year, will continue to be met thanks to the support and donations to Lycoming County United Way.
"The numbers we are seeing seeking help from organizations like the Food Bank and the American Rescue Workers reveal the somewhat unseen but very real every day dilemma being faced by a growing number of families and individuals, the basic need to put food on the table," Lowery added. "The conditions that caused these unfavorable situations didn't develop overnight, nor are we going to be able to magically solve all of them. What we must continue to do is direct the dollars our contributors have entrusted to us in the most efficient manner possible to help those affected regain some stability to their lives."
Last year, LCUW supported 44 programs in the county at 23 local agencies, helping to meet the needs of those facing an emergency; those in need of transportation, counseling, a meal or shelter; as well as meeting dental, literacy and substance abuse needs.
"While we all love Santa Claus, our work is not all crammed into one night a year. For us and those we serve providing basic human needs and the very special services required to help improve the quality of life of so many is a 24-7-365 day job description," Lowery said.
For more information about Lycoming County United Way, visit www.lcuw.org or call 323-9448.


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Darby Lee put on her University of Hartford hoodie, pulled out her hair and turned to thank her family and Lady Lion basketball coaches who helped fill Altoona Area School District's Kimmel Board Room on Wednesday for making this day possible before she got choked up and stopped amidst a flood of joy-induced tears.
"I'm so excited," Lee said.
It wasn't lost on Lee's father, Daryl, how it probably would have been different had he not moved to Altoona from New Smyrna Beach, Fla. 20 years ago.
Article Photos
Mirror photo by Gary M.?Baranec
Surrounded by her parents and coaches, Altoona's Darby Lee is overcome with emotion as she signs a Division I?letter of intent Wednesday to attend the University of Hartford on a basketball scholarship.
"It's like night and day from here and there. This is like the basketball mecca here in Altoona," said the elder Lee when asked if he thought his daughter might still be signing a letter-of-intent with Hartford or another Division I college program had she grown up somewhere else. "She still would have played down south had we lived in Florida. I don't know if the success would have been there."
Darby Lee's signing with Jen Rizzotti's college program made her the third Division I product to come out of Altoona in two years and the 40th all-time. It also was a continuation of the celebration of girls basketball excellence in this part of the state.
In addition to Lee's choice - which will cover a $200,000 education over the next four years - Portage center Olivia McCabe will sign a letter-of-intent with UPJ at her school on Friday, while Hollidaysburg's Courteney Storm has committed to IUP. McCabe had some Division I programs chasing her, too, but she decided to stay home and play for the Lady Cats, who were giving her a full ride.
McCabe came through the PA Pride AAU program and got recruiting advice from Altoona grad Sam Pierce.
"A lot of the girls I played against, in the summer time, they would be my teammates. I learned a lot from them, because each school has their little thing, so you can pick things up from another player," McCabe said. "It really helped make me the player I am."
Such is how big a deal girls basketball has become in this region. Almost every school in the Mirror coverage area can boast a player that's gone on to play at a Division I or Division II college.
The most famous girls player Portage has produced is Stacy Alexander. Alexander, of course, went on to become a big part of the group that turned St. Francis into a perennial Northeast Conference championship contender and NCAA Tournament participant.
Those Red Flash teams were heavily impacted by District 6 talent, like Alexander, Lock Haven's Jess Zinobile, Altoona's Deanna Jubeck, Southern Huntingdon's Mary Markey and Marion Center's Denise Bence.
That's what girls basketball in Central Pennsylvania is all about, what it's been all about for the last four decades. The best play it and keep getting challenged and pushed to make themselves even better.
Lee's dad was a basketball player and had a basketball in her hands from the time she could walk. But Darby Lee recognized the influence growing up that playing in this hoops hotbed had on her.
"This is a huge basketball area. Just the competition helps you in the long run, playing against all these other great players," Lee said.
Lee had a lot of potential role models on the court growing up. She didn't settle for just one. She chose to emulate all of them, as her dad took her to games at the AAHS Fieldhouse, Bishop Guilfoyle's Pleasant Valley gym, Hollidaysburg, anywhere there was a big game.
"I just liked being around it. It was fun for me," Lee said. "I'd see one girl do a move, and I'd just take it and try to make it my own. You learn from just watching."
It's not just the players either. Lee's coach, Jill Helsel, went through the process herself as a Lady Lion in the mid-1990s. Jubeck has guided Hollidaysburg to three straight district championships and sent several of her players on to continue playing in college. What they and others learned as players is being passed on to their players.
"She [Helsel] and Coach [Jill] Lane had a huge impact," Lee said. "My dad coached me all the way up through junior high. They got me and worked off what he gave me and developed me that much more."
Among the other people and throng of relatives in the room as Lee signed her documents was her younger sister, Darcy, a promising-looking ninth-grader who already is 6 feet tall. She said her sister still is way ahead of her in the skills department but seeing Darby fulfill her dream was motivation for her.
"It makes me want to achieve what she did and be so much more like her," Darcy Lee said. "You can go onto college. Maybe you can go on to the WNBA or something like that."
Darby Lee is planning to major in biology. McCabe, meanwhile, is focused on elementary education, which will help her live out another ambition down the road.
"I've thought about coaching," McCabe said. "I have a lot of little kids around."
And so the cycle continues.
Cmor can be reached at 946-7440 or email@example.com.


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KAHULUI - A state Office of Planning official said that developers of two large retail centers and 250 affordable housing units in Kihei should have filed a motion with the state Land Use Commission to amend conditions placed on the parcel that was initially proposed as a light industrial park.
"This is a clearly different project than was initially proposed," planner Rodney Funakoshi told state land use commissioners Friday at the Courtyard Maui Kahului Airport hotel.
Funakoshi was one of the last witnesses in the evidentiary portion of the state LUC's ongoing "show cause" proceedings on whether the retail centers and housing development proposed by Eclipse Development Group of California are in compliance with 20 conditions imposed in 1995 when the panel granted a land reclassification from agricultural to urban to former landowner Kaonoulu Ranch for a light industrial development.
The evidentiary portion of the proceeding concluded Friday. Parties will have a little more than a month to file findings of fact and responses. A Jan. 24 meeting date has been scheduled to hear closing arguments from the parties and to allow for commissioners to deliberate.
During his testimony, Funakoshi, the planning program administrator for the office's Land Use Division, said that a condition that the LUC imposed in 1995 called for the petitioner to develop the property in substantial compliance with the representations made to the commission. He noted that there were no residential units proposed in the Kaonoulu plans and that there is no light industrial use planned by Eclipse in its current plans for the property.
In Thursday's hearing, it was revealed that Eclipse is now committed to having at least 125,000 square feet of space on 11.5 acres dedicated to a home-improvement-center-type tenant, such as The Home Depot. Funakoshi responded by saying that this new tack would not "be an improvement" to the proposed plans.
He said that a home improvement type of business is "retail in nature" and that 11.5 acres is just one-eighth of the 88 acres proposed for development.
During cross-examination, the attorney for the retail centers, Jonathan Steiner, asked Funakoshi if he was aware of the one-and-a-half acre Maui Electric Co. substation in the project, inferring another industrial use. Funakoshi said he knew about the substation.
Steiner then asked Funakoshi what percentage of the project needed to be in light industrial to comply with the 1995 conditions. Funakoshi replied that there was no exact proportion, to which Steiner asked: How is the landowner supposed to know if it is in compliance or not?
Steiner also asked Funakoshi what would happen if retail units were built and developers did not set up an oil separator or a buffer zone between single-family homes as called for by conditions in 1995. He was apparently inferring that the separator was a component of light industrial development but apparently not needed for the current retail and housing plans.
Funakoshi said it would not be a "violation."
At issue in the case is whether any of the LUC's conditions have been violated since the property changed hands and plans have shifted from a proposed 123-lot light industrial subdivision under former owner Kaonoulu Ranch to two large shopping centers totaling 700,000 square feet of retail space under Eclipse.
Also proposed on the site is an affordable housing project that Honua'ula Partners wants to build that would be tied to its master-planned luxury golf community of the same name above Wailea.
A challenge from community groups, South Maui Citizens for Responsible Growth and the Maui Tomorrow Foundation, and Kihei resident Daniel Kanahele prompted the commission to take up the development in the context of the 1995 land use change and conditions.
Dick Mayer, a witness for the interveners and former Maui Community College geography and economics professor, who testified as an expert on economics and community planning, said that Kihei needs a light industrial area and that the current site is the best place for it.
He added that the new mall could take away from the "sense of place" that South Maui residents feel as they shop along South Kihei Road.
He said that if the industrial project use is kept, it would mean a lot more local businesses and local manufacturing, compared to a mall where businesses will be moving in, probably from the Mainland.
* Melissa Tanji can be reached at email@example.com.


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'Morell-W' | Regular Fit, Cotton-Linen Sport Coat by BOSS
Style No. 50259476
Free Shipping
This regular fit sport coat by BOSS is crafted from a soft cotton-linen for ultimate comfort with a denim finish. Contrast topstitching and a classic two-button closure complete this style. The chest pocket lining can be turned up to become an attached pocket square for added style.
Model is 6'2" with a 32" waist, 40" chest, and is wearing size 40R.
Model is 6'2" with a 32" waist, 40" chest, and is wearing size 40R.
- Regular fit
- Notched lapels
- Two-button front
- Contrast diamond design undercollar
- Open welt pocket on chest
- Chest pocket lining can be turned up to become attached pocket square
- Two open patch pockets at waist
- Four-button detail on sleeves
- Contrast trim on cuff placket
- Interior pockets: Two welt pockets with contrast trim and button closures, one open pen pocket with contrast trim
- Partially lined with diamond print design
- Double back vents
- Back length: 74 cm (29.1 in.)
- Cotton-linen


text:
After more than a month of silence on a shooting at a west Greeley residence, the Federal Bureau of Investigation says agents had surrounded the house before the subject of a bank robbery investigation shot himself to death.
Steve Hahn, 42, was being investigated in connection with the Feb. 22 robbery of a Bank of Colorado branch in Johnstown, according to the FBI. He was at a home in the 5900 block of 21st Street on March 7 when agents surrounded the residence. Coroner Mark Ward said Hahn died at home from a self-inflicted gunshot wound.
The agency has not named any other suspects in the robbery, and the investigation is closed.


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Top Definition
Cough medicine with alcohol and codeine. In the 1960s and 1970s, soldiers complaining of a cold were issued a small bottle of cough medicine. It contained alcohol an codeine. Many drank it down for the buzz.
Go to the medics and get yourself a bottle of G.I. gin. It's great on a cold day like this.
He is useless once he gets some of that G.I. gin.
He is useless once he gets some of that G.I. gin.
door Uncle Thor 15 januari 2013
Trending Now
Can you define these popular missing words?
to dance tight in the dallas fortworth area
those agg town steppas can gig
door shamack 29 augustus 2003


text:
Call our customer service representatives and get expert advices for your Dremel tool.
Contact our customer service representatives and get expert advice for your Dremel tool
Packed with power, innovations and features, the Dremel Multi-Max™ MM30 oscillating tool kit gives DIY users professional performance. Its unique integrated wrench makes accessory changes simple and you'll never loose your wrench again. With a wider angle of oscillation, a higher speed range and more powerful motor, the MM30 is capable of handling all the applications of the MM20 and more. The MM30 multi-tool kit gives you a solid performing, comfortably operating oscillating tool, as well as a wide range of accessories that can help you make quick work of all your projects. Two year warranty.
This tool is available as part of a kit.


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Once again, the Incline girls tennis team split two matches this past week, with an impressive win at Carson followed by a loss against a strong Douglas team.
Anne Schrage and the doubles team of Amanda Pretti and Tara Lincoln led the way against Carson, recording three wins each. Ginetta Sagan and Toby Felman each recorded two wins against the Senators.
Sagan followed up her strong performance against Carson with three more wins against Douglas. Kassidy Garland and Vivi Lucero, Felman and Schrage each had one win against the Douglas squad, which is 8-1 on the year.
The Highlanders' final regular-season match is against Wooster on the road Thursday at 3 p.m.
"We are really looking forward to the regionals in both the team and individual competitions," said Incline coach Jim Giancola. "There is a 1A Division for the first time for the smaller schools, and we are hoping to compete well against the teams from schools more our size.
"That, coupled with the fact that we have lots of relatively inexperienced players who are really improving each week, gives us some confidence going into the postseason."


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An effeminate man with a high pitched voice. Typically is a cat person who likes men. Their sense of fashion is shity at best. Their standard for women is low, as they could pick up a she-male and be happy. Occasionally squeaks when nervous or afraid. Aspires to be a pastry chef by the name of Betty.
Did you hear that guy who kept trying to feel me up?
Yeah, he was a real mike!
Yeah, he was a real mike!
by catcity88 December 04, 2010
Trending Now
Can you define these popular missing words?
Top Definition
The most amazing boy in the world. He is quiet around the masses but he opens up around the one he loves. He is extraordinarily protective in the best of ways. Mike can and will make you laugh harder than anyone else. He is the most adorable, cute, nice, sweet, kind, generous, loving, caring, genuine, funny, considerate, awesome person I have ever met. Every moment of my life would be better if I could spend it with him. I could talk to him all day long and we'd never run out of things to say. His smile can make my day; even if it's from across the room. I love him more than the sun, I need him more than breath it's self. I can't imagine my life without him; it scares me more than anything. I will spend the rest of my life in his arms.
Me: Mike bought me a dozen roses for my birthday!
Best Friend: JEALOUS!
Me: What can I say, he's Mike.
Best Friend: JEALOUS!
Me: What can I say, he's Mike.
by Your Emmie March 14, 2009
the coolest kid you will ever meet, he also has a fairly large dick. he is liked by everyone
he look at the mike over there bet he has a big dick
by mike the barbarian October 15, 2008
Mike is a great guy. I know many Mike's and most of them are very nice. I am going to marry one.
Also. The MOST common English Male Name.
Also. The MOST common English Male Name.
I love you, Mike.
Hey Mike! How's it going?
Hey Mike! How's it going?
by nightra February 26, 2009
Mike ; synonym's : "perfection" "godlike" "sexy" "ridiculously good in bed"
Commonly known as the most interesting man in the world, there is never a dull moment with a mike.
If I say more.. I might get wet. We'll keep it nice and short.
I love mike so much, it makes me crazy
Commonly known as the most interesting man in the world, there is never a dull moment with a mike.
If I say more.. I might get wet. We'll keep it nice and short.
I love mike so much, it makes me crazy
Last night was incredible .. i slept with mike.
by IloveMrPerfection December 13, 2011
Mike is often found riding a giant Wildebeast, shooting cannon balls from his nipples and laser beams from his eyes. 99.9% of the time he is the most stunning looking man in the room. He is funny, sophisticated, and loves poon tang. He can kill you with just a look. Everyone wishes they had a friend like him. Mike can also blow your mind with his mind! Mike has the prettiest smile, a velvet touch, and a thick dick.
"holy balls my mind was Miked!"
"my vag has been sore all week because over the weekend, I got Miked so hard"
"my vag has been sore all week because over the weekend, I got Miked so hard"
by thejunk47 February 02, 2010
What you believe to be the average guy. But beneath this quiet boy, beyond what the eye can see, there lies a hero inside. One that if you get to know, can show you love, make you laugh, and give you a great time that no one else can do. Because make makes sure he does not become one person but is always evolving to connect to the one person he wants to share the moment with. Mike is a friend like no other, always putting himself second. Mike is only great because of the people around him, without those who make him great, he is nothing. He tries his hardest to make You happy. He gets down just like everyone else, but he always must make sure everyone around him is happy first. therefore, he makes a great party favor. A Mike will save the world.
"Mike will listen, I always know he always will".
"Hey invite mike!"
"Hey invite mike!"
by M1ke C August 22, 2009
Name is often referred to as 'God of the chinese'..
and i agree.
and i agree.
by afjekhf December 15, 2008


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Allen indoor football league team gets new name
The Allen indoor football league team that signed Terrell Owens washed its hands of that era this afternoon, announcing itself as the Texas Revolution with a commitment to community involvment.
The team formerly known as the Allen Wranglers will still play in the Allen Events Center. But it's now run by Tommy Benizio, who is president/CEO of Plano-based Championship Sports Group.
"I learned a lesson many years ago that any time you can turn a spectator into a participant, you win them over," said Benizio, who served as commissioner of the Indoor Football League before coming to Allen.
The public already has its fingerprints on the team. Plano resident Todd Carlson received $5,000 by Championship Sports Group, which invited the public to name the team.
The team played last season under the direction of Jon Frankel, who left the team at the end of the season. Frankel owned the rights to the name, which brought about the mascot change. Frankel signed Owens, who lasted eight games before he and the team parted ways in May.
The team is now run by President and CEO Tommy Benizio, who previously served as commissioner of the Indoor Football League before coming to Allen.
Read neighborsgo reporter Hannah DeClerk's Q&A with Benizio here.


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Christmas tree going up on downtown Erie marquee
One southbound lane of State Street in Erie is closed this morning as crews prepare to build a 36-foot-tall Christmas tree on the Boston Store's main marquee.
UPMC Health Plan is paying $26,000 for the artificial tree, which will be used annually. The tree will be lighted Friday at 6 p.m. during Downtown d'Lights.
The site foreman said the crew will be working all day.


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Training for Open Water in the pool
Open water swimming can be a bit of a shock to the system if you're not fully ready. But luckily, there are plenty of ways to prepare for open water in the comfort of your own pool.
When swimming a long distance, it is vital to be comfortable with your stroke.
The majority of open water competitors will swim front crawl so start by making sure yours is in good shape.
A few tweaks to your front crawl technique could make huge differences to your stroke efficiency and save you valuable energy for that final 200 metres!
Try incorporating some stroke work into your swimming sessions - get into the habit of counting the number of strokes it takes to complete a length then aim to reduce your stroke count but maintain a similar speed.
It also helps to have a back up stroke to swim easy with if you need to recover.
Open water swimming is no splash and dash. In fact, most mass participation events are at least 1km (that's the equivalent 40 lengths of a 25m pool or 50 lengths of a 20m pool).
- Before attempting your open water swim, you need to be confident you can complete the distance and although many lakes are no deeper than chest height, it is useful to be able to swim the distance without putting your feet down.
The best way to prepare is to make sure you can comfortably swim FURTHER than your open water distance in the pool.
As a newbie to open water, you'll probably want to stay outside or at the back of the pack in your event which might mean swimming further than the stated distance.
- If you're a bit daunted by the distance of your swim, gradually build up to it using the challenges section of MySwimfit.
Choosing from a selection of rivers, lakes and coastlines of mainland Great Britain, you can log the distance of each swim and track your progress on a Google map in MySwimfit.
You don't need to swim 1km straight away - just gradually build up your distance as you feel more comfortable swimming non-stop for longer periods.
So you're front crawl might be poetry in motion and you might have the endurance of the Duracell Bunny but you need to be prepared for a few more surprises the open water will spring on you.
And luckily, you can prepare for most of these challenges in the comfort of your own pool.
TECHNIQUE: Sighting
- You may not know it, but without the lane lines and ropes the chances are you wouldn't swim in a straight line in the pool. The bad news is in the open water you won't be able to see the bottom of the lake and you will want to stear as far away from the banks as possible.
- All this means you need to look ahead of yourself during your swim to find a marker in the distance then keep looking up to make sure you're heading towards it.
- Looking up is a fairly simple thing to practice in the pool - try perfecting it so you don't disrupt your rhythm.
- Practise swimming in a straight line by (while supervised and in a safe environment) closing your eyes while swimming and seeing whether you naturally swerve off left or right (most people do). Try tweaking your stroke to straighten your natural line.
TECHNIQUE: Deep water starts
- There is no wall to hold on to or kick off in open water so get used to starting from treading water.
- You may need to tread water for a while before the start of an event so it's a good idea to practice being able to do this in the deep end of your pool without using too much energy or raising your heart rate.
TECHNIQUE: Turning
- Very few open water swims are completely straight line from start to finish - most will involve turning around a marker buoy, often four or five times a race.
- If you have space in the pool and a willing practice-mate (you could take it in turns?), swim up to and round your friend without touching the walls or bottom of the pool.
TECHNIQUE: Breathing both ways
- Breathing on alternative sides is recommended for pool swimming anyway, but in open water events it may become a necessity.
- If you're swimming side-by-side with another competitor, it's preferable to breathe away from them to prevent splashing or being caught in the face by their arm.
- Breathing both ways may not feel natural at first, but focus on your technique and it will become more comfortable.
- Let your head and spine join the rotation of your shoulders, inhale sharply then turn your face smoothly back in time with your shoulder rotation.
TECHNIQUE: Group swimming
- Larger open water events can be somewhat chaotic with scores or even hundreds of people swimming in close proximity to each other.
- Needless to say this will come as a bit of a shock the first time you swim in an open water event so you may want to try and stick to the outside of the pack.
- However, practising group swimming with four or five of your friends in one lane of the pool will certainly help you get used to the feeling.
Got any tips you use to practice open water in the pool? Let us know below.


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Full Retreat Program
OPA will host its Annual Astrology Retreat October 22-25, 2015 at Asilomar retreat, (close to Monterrey), California.
This retreat is designed for all levels of expertise, from beginners in astrology to professional practitioners.
4 days of Astrology magic...don't miss it!
This promises to be once again an incredible learning and community experience.
Click to Download the Full 2015 Program Brochure
OPA Retreats are a multi-dimensional experience: from small group immersions, to conference style presentations, nature and community, you stay focused and inspired the whole time.
Choose your Track: Part of the retreat involves immersing yourself in a track of your choice. You work with a highly skilled track leader and a small group of peers (max 10 people) for fifteen hours! We go deep! See the list of tracks available on this website or on the brochure (above link).
Day One at the Conference revolves around a theme: 5 Presenters discuss the theme in turn, and then the whole audience breaks down into small groups to continue exploring the theme. This year theme is: Transition from the Cardinal Cross to the Mutable Cross.
Short 20 minute presentations are fun and very informative. This year's subjects: The Astrology of Twins, Astrology and Kabbalah, The Death Chart, and Unaspected Planets.
Look forward to our Panel: 4 astrologers, using different approaches in astrology, will analyze one same chart.
What a great way to directly learn from the diverse uses of astrology, and immediately see what each perspective highlights.
Saturday evening is celebration time! The community gets together, an inspiring keynote speaker, music, and a raffle with incredible prizes: Astrology Software, free readings, a trip to Neptune...and much more! This
Do you feel it is time to deepen your astrology knowledge, expand your network, and connect with your tribe?
The OPA retreat is all the Astrology your soul needs!celebration is not included in the price, so make sure to book ($30). You can purchase the Saturday Evening Celebration ticket as an add-on to your retreat purchase, or purchase
Your Saturday Evening Celebration ticket as a separate item here.
Register Online Now
or Download Print & Mail Registration
If you have any questions about the OPA retreat, please contact:


text:
TWC9: Windows Store opens (with Snippets too), VS 2012 launches, Web Matrix 2 RTW's and more
- Posted: Sep 14, 2012 at 3:45PM
- 5 comments
Loading user information from Channel 9
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Loading user information from MSDN
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Loading Visual Studio Achievements
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Right click "Save as…"
This week on Channel 9, Brian and Jeff Burtoft discuss the week's top developer news, including;
Picks of the Week!
Oops, something didn't work.
Hello Brian,
Any plans of updating the Visual Studio 2012 ALM virtual machine from RTM to Release version?
Why aren't there more Video tutorials on building Windows Store Apps? It's really a shame. :(
13 hours ago, jacob_suibowen wrote
Hello Brian,
Any plans of updating the Visual Studio 2012 ALM virtual machine from RTM to Release version?
RTM is the released version.
Thanks for the shout out on the new Modern UI Icons site Brian! Hopefully I can get even more added as developers jump into Windows 8 UX.
Comments have been closed since this content was published more than 30 days ago, but if you'd like to continue the conversation, please create a new thread in our Forums, or Contact Us and let us know.


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The new L'Atelier de Le Creuset on the Ashley River is like something out of a chef's dream.
A brief history
Le Creuset — "the crucible" — started in Fresnoy-le-Grand, France, in 1925, after a casting specialist and enameling expert began coating iron cookware with a porcelain enamel glaze to improve versatility.It began exporting to the U.S. in the 1950s and opened its U.S. distribution center in Hampton County near Yemassee in 1977, citing easy access to the ports of Charleston and Savannah and I-95.In 1995, Le Creuset expanded into new categories: stainless steel, stoneware, silicone, enamel on steel, textiles and forged hard-anodized aluminum. It has operated a retail store on King Street across from Charleston Place for years.
A glass display case holds fondue pots from the 1970s, and other cookware dating back to the 1930s, just a few years after Le Creuset began making its colorful cast-iron containers in northern France.
Across the hall, through more glass, is the atelier, which means workshop in French and here translates into a state-of-the art show kitchen with cameras that will stream cooking demonstrations by Charleston chefs around the world.
Around the corner, a sleek office features racks of prototype spatulas, whisks and pepper grinders. Keep walking and you'll pass through the window-walled desk farm where the cookware maker's North American marketing and design employees will work.
And then there's the river-view deck outside, for relaxation, and the gym upstairs, to work off whatever's being served from the kitchen.
On Thursday afternoon, for the facility's grand opening, that was Chef Robert Carter's peanut butter caramel and red velvet cakes. Carter, who serves Le Creuset-inspired dishes at both of his local restaurants, called the renovated former Landry's Seafood House a "world-class place for world-class products."
"It brings validity to Charleston," he said.
The L'Altelier expansion at 116 Ripley Point Drive is now the company's North American marketing headquarters and culinary center. It's been in the works for about two years and under construction for since last spring. The company had outgrown its former digs near the S.C. Aquarium. Le Creuset bought the former Landry's for $4.15 million in February 2011.
Company officials noted the new facility is the only one in Le Creuset's inventory that will have both a public component — the kitchen will host students and events — and private offices.
Twenty-four of the company's 600 North American employees will be based at the new spot, including the Web and product design teams.
Le Creuset's American CEO Faye Gooding and Charleston Mayor Joe Riley helped cut the orange ribbon on the repurposed building. Both called the culinary company's continued presence in a food-crazed city like Charleston "the perfect marriage."
Reach Brendan Kearney at 937-5906 and follow him on Twitter at @kearney_brendan.


text:
A hat can be used as another term for "ass" or "butt"
Yo that dawg has a nice hat.......I want to superman that shit!
Buckweedによって 2005年05月13日(金)
1. Jimmy hat.
2. The foreskin of an uncircumcised male.
2. The foreskin of an uncircumcised male.
I put on a hat before I had sex with her. Fortunately, she didn't mind that my gentleman wears his hat indoors.
Brown Sugarによって 2003年03月18日(火)
In ultimate disc but possibly in other sports, a tournament or entire league session in which teams are made at random--i.e. chosen from hat.
"Are you playing in the hat tournament this weekend?" "No, but im playing in the fall hat league."
caliphateによって 2008年07月29日(火)
The exchangable lid/bowl for the beast. Different size hats hold different amounts of weed.
"Man, we got a lot of people hitting the beast. Throw on the 'party hat' will ya."
hollistercoinerによって 2004年02月06日(金)
Slang for hot, attractive, sexually appealing
"Have you seen Ashley's new Facebook profile picture? That shit is HAT!"
El Christoによって 2009年01月15日(木)
Hats stands for "Huge Ass Tit's". To be used in a situation where only an inner party is familuar with the expression to point out a woman with large breast.
"Damn! look at those hats in front of us!"
Aaron "-...BBG...-"によって 2006年09月10日(日)
differing pronunciation of the 'hot.' Usually used when the individual in question is so fine they're beyond the definition of the word hot.
dem beetchis ees hat. So hat I denk I's gonna splooge. Oh yer.
Willによって 2004年04月08日(木)


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Gunslinger Doc Holliday dies, the X-ray is discovered by accident, John F. Kennedy is elected president, the Florida recount begins, and the "Guitar Hero" craze is born, all on this day.
Tracking Matthew
On this day: November 8UPDATED 2:00 AM EST Nov 08, 2015
Slideshow Central
CommentsThe views expressed are not those of this site, this station or its affiliated companies. By posting your comments you agree to accept our terms of use.blog comments powered by Disqus


text:
Thanks for helping us sort this video!
Thanks for helping us associate the correct Pornstars to this video!
Help Us: Do these Pornstars appear in this video?
Pornstar Name:
I am very straight, but this girl could lick my pussy any day, mmmmmmmmmmmm I would cum very hard. You wanna watch and fuck me afterwards, mmmmmmm
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I want to get my pussy licked just like that!
I change my mind there is no way I would want them to have mercy on my pussy this is the best pussy eating I have seen in a very long time so good it makes my pussy melt into my panties...
Let me lick your pussy, it will be good mmmm Do you like my tongue flicking over your clit? Think about me doing it.
Cute video. Although.. The comments posted here are a lot more arousing. Going down on a girl was my first sexual experience.. And it seems like it will stay rooted in my mind forever.. I honestly struggle to want to do anything else with a girl.
Like watching her wet pussy rubbing as she watches. gets my pussy soaking!
I sent this to my girly girlfriend . She loves to cum to watching pretty blondes eat and fuck each other. I want to watch her 69 with another hot blonde then kiss and lick the cum off her as the other girl licks and fingers her ass.
The blonde girl sure know how to ea pussy. I wish she can eat my wet hot pussy right now!!! yummyyyyy!!!!
Lovely tits I can't stop finger fucking to this these two have no mercy on my pussy mmm sensual little pussy teasers!
Bobbie, what wasn't printed? I am waiting for you... and yes, I will tie you down and lick and finger you til you scream. Met a guy with a HUGE dick - he wants to fuck both of us! ;)
This lush lovely pussy keeps making me come back for more! Blondie-Bridgette is making me fall in love those tits are perfect and the slow to the point teasing pussy eating is mind blowing its so hot. My pussy is never going to recover from this clip. Bravo! Ladies you are so hot you have made my day!
wow, they can fuck anytime. I am so wet and up for it now. I have been fingering myself all the way through. I have cu number of times. Would love one of them to be here doing it to me
Finally i have found the hot blondes name after searching for about 2 HOURS, her name is ANETTE DAWN! I'm going to finger myself to her all the time now that I found her she is so fucking HOT!
Would love the blonde to lick my pussy. She has the best tongue flicks I've ever seen in porn. Have never been with a woman. Girls, how would you lick me out for my first time? I want ALL the dirty, juicy pussy details. Would you finger fuck me first and then suck my cunt? Tell me to make me wet.
I love the girls in this video and would love to slowly lick both of those gorgeous pussies and then have my Boat man lick their juices from my face as those gorgeous girls taste my pussy.
This is just a great vid. Shame it's not longer.Great orgasm by the blond at the end.Love the way you can hear her orgasm building up...Fantastic!
Wow.. That is anthor hot video between Sandra Shine and her friend that I watched today. An I got hard watching it. If I didn't have a appointment this afternoon I get my masturebation toy out. An start going at it watching more of them together. An what really got me going was the third on the chair finger fucking herself. I would have dropped to my knees and started fucking her. An let her keep watching the other two.
I would love for sinn to come over to my house !
Tht blond sure cn eat sm pussy omg
Tht blondie can eat the shit out sm pussy omg.....
It's beautiful because she knows how to enjoy a good orgasm
Oh fuck Ben! You can eat me out.
Damn wats da bright one name
My pussy was soaked in seconds...would love to have my face and fingers buried in all three of those hot pussies.....
Sandra's pussy is absolutely beautiful.
10 on 10
Love you trish XXXXX
Sandra Shine
very well done, so sexy and erotic
Dark haired chick is a turn OFF! Shark teeth yucks. Blondie is a hottie
I love it
WOW, talk about quality Kitty licking. It's just so Hot seeing beautiful Women pleasuring each other. I love eating pussy, totally love it, so to see beautiful Women doing it as well if just a huge turn on. I would have loved joining that group and am highly confident I could have had them all screaming. I would love to watch such a session, live.
Who loves pussy like I do?
i need to have my pussy licked just like that
Gala im waiting for tou
Nasty hairy girls! Ishhhhhhh
love the narration. hot. they should all be narrated!
hot hot wow 5/5
How do we get these girls to shave???? ISSSHHHH
anette dawn is freaking beautiful
Unlike some of the other videos i have got to applaud the camera-work.
des bonnes filles que j'aimerai bien tringler avec toi vanessa si tu as toujours la chatte humide
i'm more into watching Sandra rub one out than the other two
I would give them a baby ....
oh yes lesbian
02:07 wow wow wow !!! o_O
hi nice your boob
Bobbie, want your wet pussy eaten? I want to eat you, lick you so bad. miss you.
Yeah for real. Fuck the Sandra chick, I wanna know who the blonde is. OmFG she has the ABSOLUTE and LITERAL best "moan" of ANY bitch on the planet. She sounds so fuckin' adorable I hardly looked away from her face, I was in an almighty "awe" at the sounds she makes. PLEASE SOMEONE TELL ME who the blonde girl is!!!!!!!!!!!!!!
I wanna be there
Boys, take note of how Annette does it. ;)
love yuo sandar
Steph...twice now not printed.
What a lovely sight and a reserve girl too
Would like to practise denial, just sitting near the watching girl and both masturbate without touching cock or pussy of the other. Only fingers in each other's asshole
Bobbie, are you shy like her or more aggressive? Do I need to tie you down? Is your pussy as sweet as this?
Love it!
man sensual n slow !!! osm !!!
solo tu sai farmi godere. i tuoi video mi fanno impazzire--
the blonde is Anette Dawn the brunette Sandra Shine
annette dawn
I want to fuck Sandra Shine for the rest of my life!
The brown haired girl acted as though she was apprehensive about doing it but didn't dare to walk out. While she was sitting in the chair playing with her pussy, her babydoll panties didn't even get wet before she took them off. She was really hot.
Name of blonde?
nice nice nice
What a nice quality video - and what a pussy.
Sandra is the cutest and sexiest woman alive.. o.o....


text:
Penn State Shenango to Hold Hospital Nursing School Program Open House
Interested in a career in NURSING? Penn State Shenango will hold a Hospital Nursing School Program Open House on Wednesday, November 6 from noon to 1 p.m. in the Shenango campus auditorium located on Shenango Avenue across from the Sharon Post Office.
Representatives from Sharon Regional Health System, Jameson Health System, and Mercy's College-St. Elizabeth Health Center will be there to provide an overview of their programs, admission requirements, entrance exams, and filing dates.
There is no cost or commitment to attend. For more information, contact the Penn State Shenango Career Services Office at 724-983-2969.


text:
This year will be my first year running the Peachtree Road Race. Naturally, I wanted to start in style. That means that I don't want to start in the back with the non-runners. I'm a runner now after all, right? To get in Time Group 1A you need to finish in less than 50 minutes. To get into Time Group 1B you need to finish in less than 55 minutes. My best 10K at this point was about 56:07 so the B group was my best shot.
Enter the trusty FIRST group and their training plans that led me to success with my first half-marathon. I modified the FIRST 10K training plan to work for my shortened time frame. So I spent about 8 weeks in December and January training for a January 19th 10K with the Atlanta Track Club. Let me tell you, that plan kicked my butt. I have never done so much internal whining in my life (Why do I have to run a 10-mile tempo run for a 6.2 mile race again?).
In any case, I finished the plan and come race day…I chickened out. There was supposed to be an ice storm…the race was all the way in Peachtree City (1:30 away from my home)…it was cold outside…you name the excuse I made it. All that training for naught, huh? My second chance came in the form of the Silver Comet 10K. Now this was a plan. I love running out at Silver Comet – it is a nice flat trail and there are so many points of interest. The miles fly by for me out there.
The weather report calls for cold weather and snow flurries on Saturday but that won't happen, right? I mean it was 70 degrees outside on Thursday. I was so wrong! Saturday dawns ridiculously cold. I forgot my hat in my rush to leave the house and as I arrive in Mableton for the race it starts to snow. As I get into line to get my number, I could hardly anything with the wind and snow.
Despite the weather, the runners are in good spirits and we hit the starting line. I cue up my Nano and start the race. This time around I have a real strategy. My goal time is 54:00 so I used an online calculator to print out the mile split times and my goal pace (8:41 min/mile). I vow that no matter how tempted I am I will not go more than 15 seconds faster than my pace. I tend to peel out and go hard and then totally run out of gas in Mile 4 or 5 – not today.
I kept a close eye on my Polar every few minutes and fell into a comfortable pace at 8:30 min/mile. I am feeling really good about the race and know that I will reach my goal. The cold starts to become less of an issue as I get into the miles. In fact, I have to peel off my gloves and unzip my jacket a bit. My technical t-shirt, fleece and windblocking jacket seem like way too much clothing.
When the volunteers call out the splits at Miles 2 and 4, I am happy to find that each time I am about a minute or so ahead of my overall goal. This is fine because I am still sticking to my 8:30 min/mile pace goal and I don't feel tired at all. I'm running the Silver Comet path that I have run so many times before and I just feel comfortable. At Mile 6, I'm still exceeding my target and I still feel great. For the first time I have not walked one bit during a race. I dig in and push a bit harder since this is the last mile. Eminem's "Lose Yourself" kicks in just in time with .2 miles to go – that is my favorite running motivation song!
I cross the finish line at 51:09 and I am sooo thrilled! My last 10K (run while sick as a dog) was 56:09. I beat that time by 5 whole minutes. Maybe there is something to those FIRST plans after all!


text:
Who can you trust?
That's the question that immediately came to mind when I watched Lance Armstrong admit to doping, cheating, etc., during his interview with Oprah Winfrey that aired Thursday and Friday.
Armstrong is a man who captivated all of America, not only with his seven straight Tour de France titles, but with the way he triumphed over cancer to do so. He made Americans give a crap about cycling, when normally, most people could care less.
Article Photos
His Livestrong Foundation raised millions of dollars for those affected by cancer, many of us, including myself, wearing his bright yellow wristbands in support.
So when the allegations poured in that Armstrong was doping, that he was a cheat, I didn't believe it. Even as more and more of his competitors accused him of cheating, I still didn't believe them.
I was wrong. I was duped. I'm peeved.
Armstrong is a doper. Armstrong is a cheater. Armstrong is liar.
His seven Tour de France titles and all of his success mean nothing. As he himself put it, it was "one big lie, a lot of times."
And a lot of people were trampled during the whole process, from the fans who believed in him to the accusers he demonized and trashed.
He referred to himself as a "bully" during those days of doping, but his actions were disgraceful.
When asked if he felt wrong about his actions when they occurred, he said, "No."
When asked if he felt bad about what he was doing back when he was doing it, he said, "No."
And when asked if, back then, he felt like what he was doing was cheating, he said, "No."
After each "No," he followed with some version of scary to describe his thoughts during that time period.
So, basically, for a large chunk of time, Armstrong had no moral compass whatsoever. To him, what he was doing was no different than what most cyclists were doing, so it was Ok.
That's like a child's response when they get in trouble for doing something they're not supposed to - "Everyone else was doing it."
That doesn't fly with me, and it shouldn't fly with anybody else. Just because a lot of people are doing it, doesn't mean you have to do it.
I don't care what he's done as a humanitarian - yes, he did some good things with his foundation - what he did can never be forgotten.
Certainly, it will take years for people to forgive him for what he did, but I trust people will eventually forgive him for his mistakes. If anything, America is a forgiving country.
However, I doubt many people will trust him in the future.
Who can you trust?
It's getting harder to answer that question. If a guy like Armstrong, who had the perfect comeback story, can lie and cheat for so long, anybody can.
I remember being told at a young age that, "Cheaters never win, and winners never cheat."
Unfortunately, we're in a time where winning is everything. People cheat now, and deal with it later.
So in a way, cheaters do win in the short run, but they lose in the long run - sometimes everything.
And that's not good for sports.
We're in an era where nobody can really be trusted, and this year's Baseball Hall of Fame voting is the perfect example.
For the first time since 1996, no one was elected to the hall of fame.
Why? Suspicion. Among those not elected were Barry Bonds and Rogers Clemens, all accused at one point or another of steroid use.
None of their steroid use has been proved, yet most people just assume that they did cheat.
Do you trust that these guys are telling the truth?
Apparently, a majority of the voting baseball writers didn't, because neither was elected, and neither will be elected for several more years, more than likely.
Cheating has been going for years, and anyone who thinks attempts at cheating still don't exist is very naive. People want instant gratification, want the big prize at all costs.
But that doesn't mean its right.
And for those who think that they'll never get caught, you will. Cover-ups are undercovered and lies are always brought to the surface.
In the end, cheaters never win.
Just ask Armstrong.


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After enough strange days a normal day seems strange when the strange days seem normal.
Bob: This is the first normal day after the chaos of the holidays/wedding/hospital stay/house fire/work re-organization and it seems... weird.
Jane: It's a lola day.
Jane: It's a lola day.
av fuzzygreysocks 22 januari 2010
Trending Now
Can you define these popular missing words?


text:
As Immunotherapy Revolutionizes the Cancer Treatment...
Wall Street Decline Takes Its Toll...
Quadruple Helices Appear To Have Role...
Expanding Use of NGS Has Largely...
Proteomics has undergone the same existential choice, and self-examination, as other biological sciences with respect to automation. On the surface, automation helps laboratories cope with very high-throughput workflows.
Return on investment can be swift and convincing in an era where large testing laboratories seek to do more with less. Even medium-throughput labs can be convinced that the assay accuracy, consistency, and reduced human error (and its consequence—rework) that robotics bring make an even more compelling case for automation.
What about discovery-stage science labs operating at the lower echelons of throughput? Suparna Mundodi, Ph.D., global product manager at Rainin Instruments, offered a glimpse into what is in store for them at CHI's "PepTalk" program on protein sciences. There, Dr. Mundodi presented the case for semi-automation, as exemplified by her company's PureSpeed protein purification system.
PureSpeed uses a semi-automated pipetting protocol resident on the company's E4 XLS electronic pipette, which processes up to 12 samples in parallel. But she said the stars of the show are Rainin's PureSpeed tips, which purify microscale volumes of target protein, at very high concentration, in as little as 15 minutes. The pipette-fitting PureSpeed tips contain small volumes of standard chromatography resins concentrated in the tip region. Protein A, protein G, IMAC, and other formats are possible.
Rainin's technologic competitors in ultra-small-scale protein purification are gravity chromatography and spin columns. Gravity entails very long sample prep times and is unsuitable for parallel processing. "You can't do three or four samples simultaneously because you have to run between columns pouring buffer."
The E4 XLS pipette draws and discharges a protein solution through the resin bed, cycling as much as needed to ensure adequate binding of protein to resin. After washing away impurities, the purified protein is eluted into a tiny volume suitable for such concentration-dependent analyses as surface plasmon resonance, electrophoresis gels, and ELISAs.
The official book on PureSpeed is it bridges the workflow gap between very low and medium-high throughput experiments. Actually, said Dr. Mundodi, the 12-channel system sits within the sweet spot for most early-stage research. "If you look at the protein purification market, 12 samples can be high throughput in many instances." Her own market research, conducted before PureSpeed was developed, suggested that the average number of samples in PureSpeed's target market was just under 10.
That tells only part of the story. The many benefits of automated liquid handling notwithstanding, it is often simpler and less costly to forgo automation, particularly in labs whose workflows are constantly changing. "Many of our customers, who may be doing expression screening on 40 to 50 clones, find the semi-automated system more straightforward, even faster, than fully automated systems."
Since it is semi-automated (or semi-manual, depending on one's perspective), PureSpeed requires no programming or extensive method development. With the inexorable drain of robotics and IT talent from many labs, semi-automation represents a welcome step forward.
"The best part is it's a pipette, which makes people comfortable with using it," said Dr. Mundodi.
The theme of accomplishing more with less recurs in most analytical work. One way to achieve this is through automation, but many managers cannot be convinced of the value proposition. From the laboratory's perspective, ROI is directly related to freeing high-level scientific talent from repetitive tasks. This was the theme of a talk by PerkinElmer on the marriage of the Janus® BioTx Pro automated liquid-handling system with PerkinElmer's LabChip® microfluidics-based analyzer. LabChip GxII takes classic SDS PAGE and automates it within a microfluidic platform.
Together, the two instruments provide automated, small-scale protein purification and characterization. The Janus liquid handler prepares samples based on filter plates, tip-based chromatography, or mini-columns. Users can then feed purified samples into the analysis instrument of their choice, for example the PerkinElmer LabChip GXII microfluidic-based analysis system.
Krystyna Hohenauer, director of biotherapeutics development, life science & technology at PerkinElmer, explained a clear trend toward biotherapeutics and the need for rapid analysis and characterization of putative products. "Related are biotech patent expirations and the development of biosimilars."
There is much analytical work to be done, she said, "but companies are not hiring more analysts. The ones who remain need much more efficient ways to characterize and purify proteins. They want to analyze for key characteristics much earlier in the development process."
The combination of sample prep and GXII provides scientists with the information to make better, more timely decisions, and free them from bench time to engage in more high-level data analysis.
The Janus can be integrated into the GXII to fully automate the workflow from sample prep through to analysis and characterization, or the systems can be set up to be completely separate. This allows the researcher to utilize the instruments to best fit their sample throughput and development needs.
Membrane-bound biomolecules present numerous challenges for analysis and characterization. One family of membrane structures, the lipid raft microdomains, are attracting attention for their possible roles in disease and wellness.
Although their precise role is still controversial, current thinking holds that lipid rafts organize or compartmentalize glycolipid-protein interactions necessary for normal biological function. "One purpose is to concentrate cell-signaling molecules," explained Susan Daniel, Ph.D., assistant professor of chemical and biological engineering at Cornell University.
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text:
Top Definition
The most amazing girl ever. She's beautiful, smart, funny, and can always make you smile.
"Why are you so happy?"
"I was just with Kaitlyn."
"I was just with Kaitlyn."
โดย Vulcan 2010 19 มกราคม 2009
Trending Now
Can you define these popular missing words?
Photos & Videos
Hot, gorgeous, sexy women. Who loves to have a good time. Gives good advise and very wise. is very caring when it comes to other peoples problems, she is very trust worthy. Loves it rough, but you better watch out because her nice church girl personality will fool you, once you get to know her your will find that there is a wild women inside waiting to show herself. Everyone wants to bang her but she's taken. Every guy wants her but they cant have her, has amazing boobs and ass. But dont touch or she will fuck your world up.
Kaitlyn is a sexy bitch
โดย church whore 23 เมษายน 2009
A young female who is wild at heart! You can usually find her doing random and crazy things with her friends. She is a very sweet girl with a big heart.
Who is the amazing girl? oh, thats just Kaitlyn.
โดย KaitlynEmily. 07 สิงหาคม 2009
Kaitlyn is a gorgeous girl, who loves with all her heart and will welcome you whenever you need it. When she says she'll always be there for you, no matter what, she means it. Dont mess with her, if you ever do anything to hurt you, she may forgive, but she'll never forget. She lights up a room when she walks in. A girl of good character. She has the heart of an angel, is a sensual, graceful dancer. Katie can be a bit clumsy which she demonstrates when she often falls off of her high shoes so be ready to catch her. Has a flare for fashion. Is usually a homecoming queen. Has a passion for life! Guys gravitate towards her. She is old fashioned and will not chase guys but lucky is the guy who pursues her and wins her heart. This girl is a sweetheart, hold on to her carefully, loves romantic scenes and laughing, her heart & laugh radiates the whole room, she's a lover not a fighter, but will fight for the one she loves
Guy: "Damn, look at her go"
2nd guy: "she gives off the energy of a kaitlyn"
2nd guy: "she gives off the energy of a kaitlyn"
โดย lovernotafighter95 20 กรกฎาคม 2010
is an extreamly sexy person who everyone wants to bang.
but she is always taken so every guy envies her boyfriend..
but she is always taken so every guy envies her boyfriend..
dude i wanna bang kaitlyn is taken.
โดย yoelisafaget 10 กรกฎาคม 2008
An extremely attractive women. Amazing body that you'll never get to see, and a mind and personality that prooves to be original and real. An amazing women.
Man One: You know that hot new girl Kaitlyn?
Man Two: Don't bother, you'll never have a chance with her.
Man Two: Don't bother, you'll never have a chance with her.
โดย Drewjr 23 เมษายน 2009
a kaitlyn is normally a girl who you can talk to about anything & everything. she's always making stupid jokes, and is very intellectual. kaitlyn's are always very active and have nice bodies.
"woa, look at that kaitlyn!"
โดย sdlkfjsdf 28 ตุลาคม 2009


text:
Top Definition
(n.) an allegedly non-profit ultra-conservative organization that advocates criminalizing homosexuality and lobbies against hate-crime legislature and same-sex marriage. The AFA has, at times, advocated gay-bashing and aheres to and publicizes the belief that all non-Christians are in league with Satan. The AFA (as it is known) owns over 180 radio stations and airs its message of intolerance and hatred through these venues. AFA members rationalize their views on homosexuality by claiming Hitler was gay (tell that to all the homosexuals who died in concentration camps)and to raise children in a Jewish household is to introduce them to a criminal way of life. The AFA submits that all AIDS patients should be quarentined.The AFA once called for a boycott of Ford Motors because Ford had offered benefits to the partners of their lesbian and gay employees. The Southern Poverty Law Center has investigated the AFA as a hate group and notes that it is "primarily an anti-gay organization."
Diane Gramely is a hate monger and spokesperson for the American Family Association of Pennsylvania.
po Blenderhead1991 Мај 14, 2009
Trending Now
Can you define these popular missing words?
A euphemistic name conjured up by a notorious group of people climbing up their moral pedastools, insisting that gays are the cause of World War II, justifying atrocites and discrimination of those non-christian, poor or Native American, and supporting the complete dissapearance of the word 'gay'.
"Don we now our gay apparel
Fa la la, la la la, la la la"
{American Family Association} Oh dear. That doesn't look right...
"Don we now our homosexual apparel
Fa la la, la la la, la la la"
Fa la la, la la la, la la la"
{American Family Association} Oh dear. That doesn't look right...
Fa la la, la la la, la la la"
po Secularist Јун 18, 2011


text:
Countdown to MIX11: All Things Digital
- Posted: Mar 22, 2011 at 2:33PM
- 4 comments
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@MarkDeFalco joins the show today to talk about his job as the web guru for the MIX conference (Oh, the pressure!). From the front-end marketing site to the back-end tooling, and of course we haven't forgotten about that oh so awesome Odata feed, Mark describes his role as the person who is always thinking about how to elevate the digital online experience. Speaking of having some virtual fun, you can earn a free pass to MIX by giving us a background! Our hippity-hops need a cool background besides the boring green screen we're bouncing in front of. Who can give us the most fun backdrop and setting possible? Who will attend MIX on us? Hash tag your entries with #MIX11 by 9am PST on Friday, March 25.
On your marks, get set, go! http://live.visitmix.com.
Oops, something didn't work.
Are you looking for an image background, or video?
@MIX Fan:Either one will work!
Now that's fun, nice job on the contest idea! Downloading video...
Did anyone win the free pass?
Comments have been closed since this content was published more than 30 days ago, but if you'd like to continue the conversation, please create a new thread in our Forums, or Contact Us and let us know.


text:
High-Tech Trash: Electronics recycling event to be held Saturday
April 5, 2013
CHARLES TOWN — There are certain items that cannot be added to the piles of overflowing trash in West Virginia landfills....
Bad Dirtman. Bad. I dropped off some items today. Thanks guys.
0 Agrees | 0 Disagrees | Report Abuse »
It's difficult to take a photo of garbage without some bum getting in the way.
0 Agrees | 0 Disagrees | Report Abuse »
What is the difference between this event and taking it to the landfill and dropping them off there?
1 Agrees | 1 Disagrees | Report Abuse »
Not sure about your question about removal of the hard drive. But please make sure you use an approved "wipe" program to erase all your data on the hard drive before you dispose of it. Otherwise your privacy data or other type documents and information can be retrieved at any time by those performing the recycle action.
2 Agrees | 0 Disagrees | Report Abuse »
Is this for everyone, please? Does one need to remove computer hard drive first?
1 Agrees | 0 Disagrees | Report Abuse »
Showing 5 of 5 comments


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The Stetson volleyball team dropped a 3-0 decision to Jacksonville in an Atlantic Sun Conference match Tuesday night at Swisher Gym. The Dolphins (5-20, 3-9 A-Sun) won by scores of 25-16, 25-20, 25-17.
Freshman Cailyn Prewitt led the Hatters (4-25, 0-12 A-Sun) with five kills and three blocks. Monique Russell picked up nine digs, Kelli Carneal tallied nine assists and five digs, and Taylor Johnson recorded three blocks (one solo, two assists). Morgan Pautsch hit .333 (4-1-9).
Sammie Strausbaugh had 13 kills to lead the Dolphins, who hit .276 for the match. The Hatters hit just .031 overall.
Stetson will visit Florida Gulf Coast at 4 p.m. on Saturday.