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Expanding Use of NGS Has Largely...
As Immunotherapy Revolutionizes the Cancer Treatment...
Wall Street Decline Takes Its Toll...
Developers of DTC Genetic Testing Are...
Effective March 16, 2013, the scope of prior art will be affected by the provisions of U .S. Patent Reform Act (i.e., America Invents Act [AIA]). In this article, AIA provisions will be compared with those of European and Japanese patent law.
An inventor, subject to important exceptions for his or her own actions, is barred from obtaining a patent if the claimed invention in the patent application was (1) patented by others; (2) described in a printed publication by others; (3) in public use by others; (4) on sale by others; or (5) otherwise available to the public before the effective filing date of his or her claimed invention. All of the foregoing categories have counterparts in European and Japanese patent law.
Earliest effective filing date of a patent application key to patentability determinations
The effective filing date of a patent application by another inventor, subject to limited exceptions, is now a key criterion for determining a prior art reference's applicability. Accordingly, a Rule 131 affidavit, for evidencing an earlier invention date, can no longer be used; however, one should nevertheless retain evidence such as lab notebooks for use in derivation proceedings, which would concern whether an individual derives the claimed invention from the true inventor.
Public use and sale novelty-destroying regardless of location
As in Europe and Japan, another inventor who sells, offers to sell an invention, or uses the invention anywhere in the world can create novelty-destroying prior art. Previously, such acts were novelty-bars only if they occurred in the U.S.
Earlier-filed later-published patent application of another a prior art bar from earliest effective filing date
AIA makes no distinction between a U.S. application claiming priority to a foreign patent application and a U.S. application claiming priority to a domestic patent application, both of which become published U.S. patent applications for purposes of being applicable prior art. An earlier-filed U.S. patent application of another inventor, provided that it is subsequently published, is prior art for both novelty and obviousness determinations as of its earliest effective filing date, regardless of whether the earliest application was filed in the English language.
This type of application could not be discovered by a prior art search because it would not have been published at the time a person files his or her own patent application. Under earlier U.S. patent law, a published U.S. application that claimed priority to a non-English language application was only prior art as of its actual U.S. filing date.
In Europe, only an earlier European national application by another inventor can be used for novelty determinations from its earliest effective date provided that it was published on or after the filing date of the later application of the inventor. A European national phase of a PCT application is also novelty-destroying prior art provided that it is accompanied by a national fee and filed in an official language of the EPO. However, under EPC Article 56, both of the foregoing applications cannot be used for obviousness purposes.
An earlier-filed Japanese patent application by another inventor, published after the filing date of an inventor, is prior art from the earliest effective date (regardless of country of origin); it can be used for novelty but not for obviousness. However, earlier-filed later-published applications having a common assignee are not prior art.
Accordingly, under AIA, the scope of prior art with respect to the applicability of earlier-filed, later-published application by another is greater than those of Europe or Japan because they can be used for both novelty and obviousness rejections. Thus, a European or a Japanese patent application that did not encounter such rejections could still face them in the U.S.
The United States Patent and Trademark Office recently published new rules regarding inventors' exceptions for their own actions and related matters. Some generalizations can be made. For example, if an inventor made a public disclosure a year or less* before the effective filing date of the claimed invention, that disclosure is not prior art. However, an inventor's second, intervening grace period disclosure of a new feature or a more specific description of subject matter previously disclosed is applicable prior art against the patent application filed within one year.
Earlier-filed later-published applications with a common assignee and filed before the effective filing date of the second patent application are not prior art. Intervening third-party disclosures published during the one-year grace period are disqualified only if the inventor's earlier public disclosure disclosed as much of the subject matter as was disclosed in the third-party disclosure. Nevertheless, because most of the world utilizes a first-to-file system, prudent inventors would still be advised to file a patent application before publishing a journal article, for example.
Inventors and corporate executives alike should consider the following points: (1) five categories of prior art are to be considered for patentability determinations; (2) the earliest effective filing date of a patent or a patent application by others is the key criterion for prior art applicability, but keeping evidence is still important in certain situations such as derivation disputes; (3) public use and sale are potential novelty-bars, irrespective of geographic location; (4) a published U.S. patent application claiming priority to an earliest effective filing date can be used for novelty and obviousness bars, regardless of whether the earlier priority application was filed in an English language; and (5) inventors still have an one-year grace period for filing a patent application, but keep in mind that such a grace period is only applicable to filings in the U.S.
Jay Pattumudi ([email protected]) is a patent attorney at Bruzga and Associates. This commentary does not represent legal advice and is merely a summary of general points about the scope of prior art underAIA and certain jurisdictions.
* Within the one year grace period, disclosures by a joint inventor, or by another who obtained the subject matter disclosed directly or indirectly from the inventor or joint inventor, are also not prior art.
Jay Pattumudi ([email protected]) is a patent attorney at Bruzga and Associates. This commentary does not represent legal advice and is merely a summary of general points about the scope of prior art underAIA and certain jurisdictions.
* Within the one year grace period, disclosures by a joint inventor, or by another who obtained the subject matter disclosed directly or indirectly from the inventor or joint inventor, are also not prior art.
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While Zika continues to garner a lot of news coverage, a Mayo Clinic survey reveals that Americans believe the country's most significant healthcare challenge is cancer. Compared to other diseases, does the possibility of developing cancer worry you the most?
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TV Watch
4 Must-See Moments from Lady Gaga & the Muppets' Holiday Spectacular
So, it wasn't a huge surprise that the "Born this Way" songstress was joined by the Muppets crew for a holiday-themed musical special that aired on ABC Thursday evening ... especially since Gaga is promoting her new album, ArtPop, and Kermit, Piggy and Co. star in the upcoming flick Muppets Most Wanted.
In case you missed the show, which featured guest appearances from RuPaul, Elton John, Joseph Gordon-Levitt and Kristen Bell, we've rounded up some of the most memorable clips below.
Lady Gaga and RuPaul, "Fashion"
Gaga and RuPaul are two fiercely dressed divas, so it was fitting that they dueted on "Fashion." Statler and Waldorf seemed to approve of the pair's performance, proclaiming, "Now I've seen everything."
Lady Gaga and Joseph Gordon-Levitt, "Baby, It's Cold Outside"
Gordon-Levitt's rendition of the holiday classic was our favorite since his 500 Days of Summer costar Zooey Deschanel offered hers in Elf.
Lady Gaga and Elton John, "Artpop"
Elton John joined the godmother of his sons on the title track of Gaga's new release ... after adapting one of his own greatest hits for the occasion ("Gaga and the Jets" does have a nice ring to it.)
Lady Gaga and the Muppets, "Venus"
Dr. Teeth and the Electric Mayhem played on this Gaga track, which also featured backup vocals from Fozzie, Gonzo, Rowlf and other plush pals, proving what we already knew: Muppets make everything better.


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Top Definition
one of the biggest drug dealers in swift current, who fucks shit up and gets his dick sucked every night before bed by some of the hottest bitches in town.
i wish i got my dicked sucked at night before bed like jarrett wandler!
von jaaarrreeettt 18. November 2010
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Can you define these popular missing words?


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on December 10, 2012 - 10:15 PM
, updated December 11, 2012 at 3:19 PM
A third of the dogs at the SPCA Serving Erie County are pit bulls.
And as other breeds around them are snatched from their kennels for adoption, the pit bulls at the Town of Tonawanda shelter wait for their turn.
And they wait.
"Pit bulls sit here for a month or more before they find a home. The others sit here for less than four days," said SPCA Executive Director Barbara Carr. "Why is that?"
Carr knows why.
The perception of pit bulls as aggressive continues to dog the entire breed, routinely creating lengthy stays for them at the facility on Ensminger Road before they're able to find a good home.
But Carr points to recent incidents that show these much-maligned canines in a much different light: as victims.
In October, there was Gotti, the pit bull who was chained to the back of his owner's SUV and dragged several blocks on West Side streets before being stopped by police.
Then there was Layla, the pregnant pit bull taken in by the SPCA after surviving a gunshot wound to the head.
Last month, it was Metro. The emaciated pit bull puppy was left out in freezing temperatures in front of a Buffalo animal hospital before being rescued by an ambulance crew from Rural/Metro Medical Services.
And just last week, authorities seized a dozen pit bulls – one injured, two dead – from a home in the Town of Brant and charged the owner with animal cruelty.
"The media has done such an outstanding job demonizing pit bulls, they have become all but invisible," Carr said. "They don't consider them kind, loving pets – and the majority are just that."
Pit bulls – or bully breeds, as they're known – actually refer to a few different breeds, including pit bull terriers, American Staffordshire terriers and Staffordshire terriers, or any combination of the three.
Known for their strength and stamina, pit bulls are sometimes illegally trained to protect drug stashes or fight other dogs to death for sport, which continues to perpetuate the pit bulls' bad reputation.
"I think they're misrepresented," agreed Kelly McCartney, director of the City of Buffalo Animal Shelter, which also takes in a number of pit bulls, "and it's unfortunate, because a dog bred in a proper environment is very, very loyal and smart."
Humane groups have seen this before.
Every decade or so, a certain breed gets labeled as a problem, Carr said.
"I can remember when it was German Shepherds. I can remember when it was Doberman Pinschers. I remember when it was Rottweilers," Carr said. "Now, it's pit bulls."
A couple of times a year, area humane groups sponsor a special campaign geared toward adopting pit bulls, but it's not always easy changing perceptions.
Inside the SPCA shelter, Carr showed off the nine pit bulls waiting to be adopted last week.
At the back of the building, there were another 17 injured or abused pit bulls that had been brought in on animal-cruelty cases.
Carr went from kennel to kennel pointing out the abuses: broken bones, pulled from deplorable conditions, no veterinary care.
She stopped in front of Layla, the pregnant pit bull, and handed her a biscuit.
"This was the one that was shot in the head," said Carr, as she pointed out the wound. "She was shot behind the left ear."
email: email@example.com


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T +420 274 810 745-7F +420 274 812 181M +420 603 232 firstname.lastname@example.com
The atelier CASUA was founded in 1991 by Ales Podebrad, a civil engineer, and by the architect Oleg Haman. Their objective was to build an atelier which would not only offer their own architectural ideas, but also their construction solutions, and this way, it would offer complex services to their customers. It is now 20 years that this objective has been successfully reached and the Atelier CASUA implemented a lot of successful projects.
Among the most recent projects are the luxury residence "Czech terraces" (Almaty – Kazakhstan), the Master Plan of the City of Usti nad Labem, Park Malsovice – leisure activities park in Hradec Kralove and the Office Islands complex in Prague – Letnany for the company Hochtief Development Czech Republic, which obtained the pre-certification of the LEED Silver Certificate for suistanable buildings.
Casua is a founding member of Czech Green Building Council
Casua has been a member of Equator European Architects since January 2007
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James W. Conley, 53, Altoona, passed away Tuesday.
He was born in Niagara Falls, N.Y., son of Thelma (McClellan) Conley and the late Carl Conley. He married Marla Coble on Nov. 24, 1980, at the Assembly of God, Coalport.
Surviving are his mother; his wife; two children: Jammie Frye and Aaron; five grandchildren: Christian, David, Aden, James and Michael; three sisters: Sharon and husband, Randy, Donna and husband, George, and Heather; and three brothers: Carl and wife, Barbra, Robert and Clarence and wife, Diana.
He will also be sadly missed by nieces, nephews, great-nieces and great-nephews.
James was of the Protestant faith.
A private memorial service will be held at the convenience of the family.
In lieu of flowers, memorial contributions may be made to the American Cancer Society.
Arrangements are by The Good Funeral Home Inc., Alto-Reste Park Cemetery.


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Top Definition
Although this person may put up a front or image of being straight, he, in fact, is an A grade faggot. Caution: Known for prostituting himself and carrying various forms of STDs. He likes booty. Also a warrior.
He is quite a Shujoy.
de Anon Dudeee 30 novembre 2012
Trending Now
Can you define these popular missing words?
1. Personified sex
2. Sign of amazingness
2. Sign of amazingness
Wow, he seems like a Shujoy.
de Anonymous persona 11 mars 2008


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Women's Tennis Loses 4-2 Match To Kentucky
LEXINGTON, Ky. The Ball State University women's tennis team fell 4-2 to Kentucky in its last match of the weekend Saturday night at the Hilary J.Boone Varsity Tennis Center.
The Wildcats took the doubles point to go up 1-0 over the Cardinals heading into singles action. Kentucky's twosome of Caitlin McGraw and Kirsten Lewis took the first match from Ball State's Courtney Wild and Ayaka Terashi by a score of 6-1 at the No. 3 position. Toni Ormond and Courtney Earnest then dropped a 6-1 decision at the No. 2 position to Kentucky's twosome of Edmee Morin-Kougoucheff and Aldila Sutjiadi.
In singles action, Bethany Moore lost to Morin-Kougoucheff at the No. 3 spot to make it a 2-0 match for Kentucky. After that, Kristel Sanders defeated Taylor Lederman, 7-6, 6-1 at the No. 4 position to make the score, 2-1. Wild then dropped her match at No. 1 singles to give the Wildcats the 3-1 edge over the Cardinals. Earnest made it a one-point match after posting a straight set victory, 6-3, 6-3 (8), over Kelsey Dieters at No. 2. With the match at 3-2, Caitlin McGraw would beat Ormond to secure the win for the Wildcats.
The Ball State women's tennis team continues aciton when it hosts Wright State and Oakland University Friday at the Muncie YMCA Tennis Center.
Kentucky 4, Ball State 2
Singles order of finish 3,4,1,2,5*
1. Aldila Sutjiadi (UK) def. Courtney Wild, 7-6, 6-2
2. Courtney Earnest (BSU) def. Kelsey Dieters (UK) 3-6, 6-3, 1-0 (8)
3. Edmee Morin-Kougoucheff (UK) def. Bethany Moore (BSU) 6-0, 6-2
4. Kristel Sanders (BSU) def. Taylor Lederman (UK) 7-6, 6-1 (5)
5. Caitlin McGraw (UK) def. Toni Ormond (BSU) 6-3, 6-2
6. Stephanie Fox (UK) def. Ayaka Terashi (BSU) 5-3 DNF
Doubles order of finish 3,2,1*
1. Kelsey Dieters/CeCe Witten (UK) def. Bethany Moore/Kristel Sanders,6-3
2. Edmee Morin-Kougoucheff/Aldila Sutjiadi (UK) def. Toni Ormond/Courtney Earnest, 6-1
3. Caitlin McGraw/Kirsten Lewis (UK) def. Courtney Wild/Ayaka Terashi, 6-1


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SALEM - City patrolman Gary Poage has been suspended without pay pending the outcome of a case against him for charges of drunken driving and marked lanes following his arrest early Sunday in Canfield.
Police Chief J.T. Panezott discussed his decision Tuesday after contacting Poage by phone and advising him of his employment status. He said he couldn't meet with him in person because Poage said he was out of the area.
Panezott and Lt. Don Beeson then traveled to Poage's residence and picked up his equipment, including his service weapon and badge. With Poage's license suspended and seized as a result of the OVI, he can't operate a vehicle, which is a requirement for police officers to patrol and respond to calls.
"You can't perform your job if you don't have a valid driver's license," the chief said.
When asked why the suspension was without pay, he made it clear that's what his practice will be as chief, saying "if you perform your duties, you get paid. If you don't perform your duties, you don't get paid."
"He really didn't have anything to say," Panezott said regarding the conversation with Poage. "I am evaluating his personnel file to decide if any other action will be taken prior to this being resolved in court."
The loss of Poage makes the department even more short-handed than it already was, according to the chief, with other officers having to cover and work overtime. He said it hurts everybody on the department.
"I hate to see it happen. He should have known better," Panezott said, adding they'll have to await the outcome. "Everybody's innocent until proven guilty."
Poage, 47, was taken into custody after a traffic stop on South Broad Street just south of Fairground Boulevard in Canfield at 2:32 a.m. Sunday. He was found to be more than three times over the legal limit for alcohol after blowing a .260 during a portable breath test. The legal limit in Ohio is .08.
He's scheduled to be arraigned today at 5 p.m. at Mahoning County Court #5 on South Broad Street in Canfield, according to records on the Mahoning County Court web site. He had not worked since the incident and has been with the Salem Police Department since June 1993.
According to city Service/Safety Director Ken Kenst, most of the offenses described in the city's employee handbook which could warrant discipline apply to when an employee is on the job or using city equipment, not to their actions while off the job on their own time.
The only offense that could relate to a situation where an employee is charged with OVI on their own time would be failure to maintain required licenses or registrations or in the case of workers who must drive city-owned vehicles, such as street workers, the failure to maintain a required commercial driver's license.
In some of those cases, the ability to legally drive is part of the job.
According to the policy, the license requirements fall under Group 3 offenses, which are considered "of a very serious or possibly criminal nature and cause critical disruption to the operation of the city." The policy said that appropriate disciplinary action for Group 3 offenses includes from 15 days suspension to discharge for a first offense and discharge for a second offense if it was not implemented at the first offense.
Panezott has talked with Kenst and Mayor John Berlin about the Poage situation and Kenst said they're following J.T.'s lead and being his support. He also said they're taking direction from city Law Director Brooke Zellers.


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The world's tallest buildings
POSTED: 09:13 AM PDT Mar 22, 2012 UPDATED: 11:00 PM PDT Sep 02, 2015
In honor of National Skyscraper Day on Thursday, take a look at which buildings are the tallest in the world.
The views expressed are not those of this company or its affiliated companies. Please note by clicking on "Post" you acknowledge that you have read the Terms Of Service and the comment you are posting is in compliance with such terms.blog comments powered by Disqus


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on December 24, 2012 - 9:45 PM
SANBORN – Chris Phillips of Wilson walked into Hoover's Dairy just before 9 a.m. Friday and, without anyone asking, made clear that he was a man on a mission: "I have to get more egg nog before it's all gone again."
Keep in mind that at this point, there was a chance the world was going to end in a few hours, so there wasn't a lot of time for chitchat. But even without looming Armageddon, if you've ever had Hoover's egg nog, you understand the urgency.
Happily for Phillips and for egg nog lovers near and far, the apocalypse was a no-show. Even better, Rob Hoover and his family were well-prepared for the steady stream of people making their annual Yule journey to the nondescript little white building on the big farm in rural Niagara County.
The Hoover name is literally synonymous with this part of the county, where the family has been farming since the late 1800s and operating the dairy since 1920.
Yet of the 12 Western New York institutions we've chronicled in this yearlong series, Hoover's is arguably the least well-known of the group outside of the immediate area. If you know it at all, it's likely by virtue of its standing as one of the few remaining independent dairies in the United States and part of an even smaller group that still delivers milk products, which it does to about 1,600 homes across Niagara County.
Although the family no longer sells the milk produced by their own cows, the Hoovers hold onto another throwback from an earlier time by bottling in glass. Once the industry standard before plastic became the preferred material for containers, glass bottles now are a rarity, but for Hoover's customers, glass is one of the main selling points. For a brief period every year, Hoover's breaks out the recipe perfected by Rob II – great-grandson of founder Edwin Hoover – fills up those glass bottles with a sweet, yellowish, creamy elixir, and, like sailors answering the siren's call, people start showing up to experience Nog-vana.
"A lot of people have told us they didn't like egg nog," Rob Hoover said. "Then they'll try it, and the next thing you know, they're coming in to buy it."
The homemade egg nog has not been a constant in Hoover history; for years, the family sold it, but didn't make it. But Rob wanted to try to improve on what was out there.
"Trial and error," he said. "My last batch isn't like my first batch."
The nog is made on site, mixed in huge metal holding tanks before it is bottled. Rob said he expects to sell about 4,000 quarts this year. When it is gone, it is gone until next year. There is nothing quite as sad as being in the dairy when one of the Hoovers has to break that news to a disappointed customer. Conversely, when worried customers hear that the egg nog IS available … well, imagine the look on a 4-year-old's face on Christmas morning.
The Hoovers have plenty of stories of people for whom it wouldn't be Christmas without some of their egg nog. There's the person who came in on a Tuesday, bought 40 quarts and then came back the next day asking for 12 more. There is the lady who lives just down the road who buys dozens of bottles and then carefully packs them for next-day delivery to her loved ones. There was the guy traveling from Michigan to Vermont – a journey that doesn't normally include the hamlet of Sanborn – who had to stop for some before continuing his trip.
But you don't have to trust them; spend a mid-December morning inside the dairy, and every customer you meet has an egg nog story they can't wait to share.
June Jordan of North Tonawanda has been coming here for about nine years to pick up a case of bottles, which she then festoons with a red ribbon to give out as gifts.
"It is the best in the world," she said.
Doris and Jim Peron of Wheatfield have been receiving Hoover's egg nog as a gift for years but had never bought any to keep for themselves. They weren't going to chance it this year.
"We decided we were going to come and get our own to make sure we get it again," she said.
John Whiteman is an orthodontist from Youngstown who has been working on Hoover family teeth for years but had never tasted the egg nog that helped them pay for all those appointments.
"I heard so much about it, I decided to make a little safari up here," he said.
The operative word there is "heard," which is pretty much the only way anyone ever finds out about Hoover's. The family doesn't really go in for advertising, either old school or newfangled. (I asked Rob and his mother, Judy, about a Facebook page devoted to the business, and both said they had heard of it but didn't give it much thought. I didn't bother asking whether either of them were on Twitter.) Judy, who married into the Hoover family, said all of her ancestors almost certainly would have resisted talking to a newspaper about the business, let alone allowing a photographer and videographer to get them on camera.
You can't argue with their success.
You also needn't worry about the future. The Hoovers run a truly family business, with multiple generations involved, including the next one coming. Rob's son Robbie is 13 years old and already is helping out after school, even more so at this time of the year. "As soon as he's off the bus he's in here," Rob said. "Making egg nog."
email: firstname.lastname@example.com


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Fun Places
Roebuck "Pops" Staples
The patriarch of one of music's most successful families, Pop Staples worked with everyone from Robert Johnson to Curtis Mayfield. Roebuck Staples was born December 2, 1915, in Winona, MS; a close friend of Charley Patton, he also played not only with Johnson but also such legends as Son House and Robert Jr. Lockwood, becoming a top-notch blues guitarist in the process. Increasingly drawn to the church, he joined the gospel group the Golden Trumpets in 1937, and upon relocating to Chicago in 1941, he signed on with the Windy City's Trumpet Jubilees; by the following decade, Staples was regularly performing at services in the company of his daughters Mavis and Cleotha and son Pervis, and soon they began appearing professionally as the Staple Singers. While originally a gospel group, the family achieved their first commercial success with a more contemporary soul sound honed during the late '60s while signed to the Stax label; by the early '70s, the Staples even moved into funk, scoring a major pop hit with "I'll Take You There." After signing with Mayfield's Curtom label, they also found success with "Let's Do It Again." Pop Staples did not pursue a solo career prior to releasing 1992's Peace to the Neighborhood, which returned him to his blues and gospel roots. Its follow-up, 1994's Father Father, earned a Grammy for Best Contemporary Blues Album. Staples also appeared in several films, including 1998's Wag the Dog. Late in 2000, Staples suffered a concussion after a fall in his home; shortly thereafter, on December 19, he passed away at the age of 85. Jason Ankeny
Contact FindTheFun.com
© 2003 FindTheFun.com. All rights reserved.


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Coffee Makers
Shop for Coffee Makers, French Press and Pour Overs
Traditional coffee makers magically mix hot water and fresh coffee grounds, but many variables effect how your cup of coffee ultimately turns out. We post real reviews and information to help you select the right preparation to satisfy your coffee cravings.
From fully automated coffee makers to hands-on manual control and cold brew in between, Seattle Coffee Gear carries a wide variety of coffee makers. Choose from French press pots, stovetop makers, pour overs and a fine selection of drip coffee brewers to make your next cup of coffee your best cup of coffee.
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DeLonghi DC514T Esclusivo Drip Coffee MakerStay fresh! The DeLonghi Esclusivo is a large capacity, programmable drip coffee maker with a special indicator that let's you know how long the coffee has been in the pot. Learn More
Regular Price: $112.00
DeLonghi kMix Coffee Maker DCM02 SeriesWhat's your personality? DeLongi lets you decide with its kMix Coffee Maker DCM02 Series in three bold colors: red, black or white. Simple yet sophisticated and functional, the DeLonghi kMix brews up a strong cup of coffee despite its petite size. Crafted in a durable die-cast aluminum case, this coffeemaker will fit just about anywhere you want and features an intergrated cup warmer and reusable nylon filter for easy use and clean up. To get brewing, just add your favorite coffee grounds. Learn More
Regular Price: $149.95
Out of stock


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on February 12, 2013 - 4:49 PM
LOCKPORT – A female bartender at Lock 34 bar told city police she feared for her safety Sunday when a man pulled out a knife inside the bar.
John W. Walsh, 29, of Washburn Street, was removed from the bar, at 80 Main St., about 2:30 a.m. and charged with second-degree menacing, police said.
The bartender told officers Walsh pulled out the knife and slid it across the bar. She told police she was afraid for her safety because she wasn't sure what Walsh was going to do with the knife.


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The former Twilight actress and American Idol alum performed three co-written acoustic songs at Erik Wahl's UNthink: Rediscover Your Creative Genius book launch, where Reed couldn't keep her eyes off her man while they were on stage.
"At the event, they talked about how they have polar opposite pre-performance rituals," a partygoer tells PEOPLE. "He's a 'rock 'n' roller' who drinks whiskey. And she isn't much of a drinker, so she just meditates and takes deep breaths before going on stage."
But one thing is for sure: The pair share a mutual adoration for each other.
"They were clearly so in love," the onlooker says. "They were inseparable the whole night. She was talking about how she hates being without him. They had some PDA going on in a booth when they weren't performing."
Soundcheck-ing in my super sweet @allsaints_ gear #UNthinkrevolution #beverlydrive @erikwahlsay.ly/tOV5YmL— Nikki Reed (@NikkiReed_I_Am) June 5, 2013
– Dahvi Shira
Spot a celebrity? Tweet us your sighting— People magazine (@peoplemag) August 7, 2012
@peoplemag with #CITA and it could appear on PEOPLE.com!


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German Corner LLC Store
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County recycling to get extreme makeover
Cans, plastics, paper and more all will be combined; curbside pickup fazed out
February 20, 2013
A new way of recycling is coming to Lycoming County....
I hope they will continue the drop off sites. I recycle but don't have a garbage pick up.
5 Agrees | 1 Disagrees | Report Abuse »
I have read this article a couple of times...I'm not quite sure I get it.
Someone help me out here. They are talking about 'customers' throwing everything in the trash, then the Trash Haulers taking it to a 'sorting center' and having it sorted? Then the rest gets hauled away to a landfill? So no more personal trips to the drop off site/no sorting, they do it all?
Am I understanding this correctly?
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I dont care what they say. The county recycling center does not lose money. They pay prisoners 25 cents an hour to sort the recyclables over over there. They might lose money on curbside pick up but they more than make up for it with what they collect both at the land fill and the drop off sites. wwhickok, they will be providing a seperate single container for you to put all your recyclables in without having to sort it. You keep it seperate from your trash. Your local trash hauler would pick that recycling container up once a month they and charge you 5-10 bucks for doing it. So the trash haulers make money off the free service you used to get and the recycling center gets near 100% profit off your recyclables. In my opinion, i think this will cause some to quit recycling. I agree with andy33. I sure hope they continue the drop off sites.
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What this equates is big money for trash haulers and the county recycling center.
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Thank you Netwarrior for explaining that to me, I read it like three times and just wasn't grasping it.
Yeah..now that I actually understand this..I'm totally against it. Why should I have to pay to recycle? That's bogus if you ask me. I agree, they 'hope that it'll increase recycling' I'm sure it'll drastically reduce it. And they can't FORCE you to do it. I too hope they keep the drop off sites, I'd much rather do that. This sort of sounds more like a way to bend over the citizens and **** them again.
3 Agrees | 1 Disagrees | Report Abuse »
Maybe that's why a new prison is needed? More discount employees for the county's business endeavors.....
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This is good for everyone. The reason you should have to pay for recycling is that you have to pay for trash disposal. The $5-10 a month you will pay to have your recycling picked up is cheaper than the $5 a bag you would pay to have it picked up for disposal. Recycling will save you money. Thast why you do it. There is no money being made in recycling. The price the county gets for the materials barely offsets the cost of collecting, processing, and hauling them to an end user. If it wasnt for the prison labor recycling couldnt exist. And for the guy that recycles but doesnt have garbage pick up exactly what do you do with your garbage?
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GE Appliances Artistry™ Series 20.3 cu. ft. Bottom-Freezer Refrigerator - Black
1 year parts and labor
The GE Artistry™ Series ABE20EGEBS bottom freezer refrigerator brings a hip new look to your kitchen. The high-gloss crown doors with horizontal stainless handles are fresh and modern. Inside are your must-have features, including easy-to-use upfront temperature controls, adjustable gallon-size door bins, and crisper drawers designed to keep fruits and vegetables fresh and tasty. The 20.3 cu. ft. black GE Artistry Series ABE20EGE bottom freezer refrigerator is equipped for an icemaker if you choose to install one. In both the freezer and fresh food compartments, bright, clear lighting makes it easy to find just what you're looking for.
- High-gloss doors with stainless handles give polished appearance with subtle curved lines and an accent of sleek stainless
- Easy-to-use, upfront temperature controls regulate both fresh food and freezer sections
- Equipped for optional icemaker to easily accommodate icemaker installation
- Crisper drawers provide the ideal environment for storing your fruits and vegetables
- Adjustable glass shelves offer a variety of shelf configurations for extra food storage options
- Adjustable gallon door bins offer ideal space for storing gallon containers in the door
- Reversible door kit included
Specifications & Dimensions
Product Documentation
Owner's Manual - English (Warranty)
Installation Guide
Energy Guide


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Let's hope it's performance art, and not a psychological problem.
That is James Franco's view of Shia LaBeouf's recent behavior, as put forth in a thoughtful, empathetic op-ed piece Franco published Thursday in The New York Times.
The article, titled "Why Actors Act Out," recaps LaBeouf's odd antics lately, including his appearance with a paper bag over his head – bearing the line "I am not famous anymore" – at the German premiere of Nymphomaniac.
Franco, 35, is not close with LaBeouf, 27, but is clearly concerned for him. And as someone who has also to deal with the sometimes unwelcome spotlight of fame, he feels his pain.
"Though the wisdom of some of his actions may seem questionable, as an actor and artist I'm inclined to take an empathetic view of his conduct," Franco writes.
"This behavior could be a sign of many things, from a nervous breakdown to mere youthful recklessness. For Mr. LaBeouf's sake I hope it is nothing serious. Indeed I hope – and, yes, I know that this idea has pretentious or just plain ridiculous overtones – that his actions are intended as a piece of performance art, one in which a young man in a very public profession tries to reclaim his public persona."
Franco points to Marlon Brando and Joaquin Phoenix (in the movie I'm Still Here) as examples of other actors who have wrestled with the pressure of living up to a public image of themselves.
Franco has struggled with it, too. "Because film actors typically experience fame in greater measure [than other artists], our personas can feel at the mercy of forces far beyond our control," he writes.
At this point, Franco simply hopes LaBeouf does have control over himself, and is not showing signs of cracking under pressure.
"Mr. LaBeouf has been acting since he was a child, and often an actor's need to tear down the public creation that constrains him occurs during the transition from young man to adult," Franco writes. "I think Mr. LaBeouf's project, if it is a project, is a worthy one. I just hope that he is careful not to use up all the good will he has gained as an actor in order to show us that he is an artist."
James Franco Empathizes with Shia LaBeouf's Acting Out
Abaca; Invision/AP; Inset: Splash News Online


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PORTAGE - No one will be confusing either Emily Chobany or Olivia McCabe with Lennay Kekua: Unlike Manti Te'o's fictitious girlfriend, Chobany and McCabe are very much for real.
So are the rest of their Portage Area High School girls basketball teammates and the Lady Mustangs chances to get a very high seed in the District 6 Class A playoffs next month.
McCabe and Chobany combined for 42 points, and seven other Lady Mustangs cracked the scoring column as Portage made surprisingly easy work of perennial 6-A power Conemaugh Valley, 70-48, on Tuesday night at Len Chappell Gymnasium.
It was Portage's second double-digit win against the Lady Jays this season, running the Lady Mustangs' record to 14-1 while snapping Conemaugh Valley's nine-game win streak.
"People actually take us seriously. They think that we can, that the last [win over Conemaugh Valley] wasn't just a lucky fluke. We can actually beat teams that people put us up against," said McCabe, who collected 22 points, 10 rebounds and two blocked shots.
Chobany added 20 points and five assists, while Katie Nolan and Lexi Wozniak sank five 3-pointers off the bench for Portage, which improved to 8-0 in the WestPAC North. The Lady Mustangs now have a two-game lead on Conemaugh Valley, with Conemaugh Township third in the section at 5-2.
"It's no mystery coming into tonight how big this game was as far as the WestPAC North standings, the District 6 standings. This is a huge win for our program in that it's a step closer to playing in those big-time championship games, which we've never had here," Lady Mustangs coach Lance Hudak said. "It's a credit to every girl in this program.
"We're playing well."
Finding an area in which the Lady Mustangs didn't perform well was like finding a needle in a haystack. Conemaugh Valley scored the first bucket, then responded to a 13-2 Portage run to get within 13-11 before a 12-0 spurt broke it open.
If there was any doubt, Portage eliminated it by carrying the third quarter, 22-9, and pushing the lead to as many as 32 points early in the fourth. Conemaugh Valley was led by Emily McCray's 10 points in falling to 11-3 overall.
"We were less than a hundred percent coming into the game, and we played far less than a hundred percent during the entire game. It was going to wear us down," Conemaugh Valley coach Teri Cruse said. "We don't let anybody score 70 points, and we let them do it at will."
In fact, Portage only scored one point in the last 6:04 with its junior varsity players in the game. Through three quarters, the Lady Mustangs made 24 of 47 shots, six of nine 3-pointers, forged a 30-22 lead in rebounds, forced 12 turnovers and only turned the ball over seven times despite pushing the tempo.
Much of the credit for that last statistic has to go to Chobany. The 5-foot-5 junior point guard kept things under control, but showed assertiveness in driving to the bucket for the layup or the pass and also made 8 of 15 field goal attempts.
"I worked all offseason just shooting and trying to be an all-around player," Chobany said. "I wanted to get more of my shots in, but I wanted to help my teammates by getting them the ball."
They were coming through when she got them the ball. In addition to nine points from Nolan and six from Wozniak - both freshmen - senior Emily McCabe scored eight.
"If we subbed five at a time or two or three at a time, there was no let-up," Hudak said. "You have kids coming in that can flat out shoot it, which we haven't had."
That's important if the Lady Mustangs are able to build on the regular season success when the postseason rolls around.
"I'm a little bit surprised, but I know we worked hard for this," Chobany said. "I'm just really happy to have a team like this that works this hard."
Game notes: The officials from the varsity game never showed up, so Marc Myers and Corey Theys stayed from the JV contest and handled both sides of the doubleheader. ... Portage's "Herd" student section was dressed up in beach attire. ... The Lady Mustangs travel to Blacklick Valley on Thursday.
CONEMAUGH VALLEY (48): Cekada 1 0-0 3, Hody 4 0-0 9, L. Stiffler 2 1-2 5, E. Smith 3 3-3 9, McCray 3 4-6 10, Miller 0 0-0 0, K. Smith 3 2-2 8, Hagerich 0 1-2 1, K. Stiffler 0 0-0 0, A. Smith 1 0-0 3. Totals - 17 11-15 48.
PORTAGE (70): Chobany 8 3-3 20, Ramus 1 0-0 2, Zatek 0 2-2 2, E. McCabe 1 4-8 8, O. McCabe 9 4-6 22, Nolan 3 0-0 9, Wozniak 2 0-0 6, Jubina 0 1-3 1, Baker 1 0-0 2, Crum 0 0-4 0, Benton 0 0-0 0. Totals - 25 14-26 70.
Conemaugh Valley11 15 9 13 - 48
Portage16 27 22 5 - 70
3-point goals: Conemaugh Valley 3 (Cekada, A. Smith, Hody); Portage 6 (Chobany, Nolan 3, Wozniak 2).
Records: Conemaugh Valley (11-3; 6-2 WestPAC North); Portage (14-1; 8-0 WestPAC North).
Officials: Marc Myers, Corey Theys.
JV: Portage, 26-23. High scorers-Kacie Smith, CV, 9; Kacie Baker, P, 11.


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After Russia meteor strike, Rep. Lamar Smith plans hearing on threats from space
San Antonio Rep. Lamar Smith, chairman of the House Science, Space, and Technology Committee, called the bus-sized meteor that crashed near the Russian city of Chelyabinsk a "stark reminder of the need to invest in space science."
He noted that a large asteroid, Asteroid 2012 DA14, is also passing just 17,000 miles from Earth today, "less than the distance of a round trip from New York to Sydney." And he announced that he'll hold a hearing in coming weeks "to examine ways to better identify and address asteroids that pose a potential threat to Earth."
The Russia meteor injured more than 1,000 people, destroying buildings and creating — let's be honest — some of the coolest dashboard camera footage in history. (Why do so many Russians have dashboard cameras?)
"Developing technology and research that enable us to track objects like Asteroid 2012 DA14 is critical to our future," Smith said in a statement. "We should continue to invest in systems that identify threatening asteroids and develop contingencies, if needed, to change the course of an asteroid headed toward Earth.
"Fifty years ago, we would have had no way of seeing an asteroid like this coming. Now, thanks to the discoveries NASA has made in its short history, we have known about 2012 DA14 for about a year. As the world leader in space exploration, America has made great progress for mankind. But our work is not done. We should continue to study, research, and explore space to better understand our universe and better protect our planet."


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Cactus Harness Latigo Laced Barrel Rein
These Cactus Harness Latigo Laced Barrel Reins offer a nice grip. Designed to offer an elegant look, they're fitted with swivel bolt snaps. Read more...
Cactus Harness Latigo Laced Barrel Reins provide a nice grip and an elegant look. Swivel bolt snaps for easy on/off bit attachment. Conway Buckles allow for rein length to adjusted. 1/2" laced rein. 7' long.
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The loggerhead shrike (Lanus ludovicianus) is a predatory songbird about the size of a blackbird. It is bluish-gray above and white below. The wings and tail are mostly black with white patches. It resembles a mockingbird but can be distinguished by its grayer color, darker wings, a shorter, darker tail, a short, hooked beak and a broad, black mask through the eyes and across the bill. In flight, the shrike has a much faster wingbeat than a mockingbird. Juveniles are paler and browner. Its song is seldom heard and consists of an assortment of short-warbled phrases and harsh squeaks. When alarmed, it utters shreaking clacking notes.
Shrikes are usually seen perched along roads on fences or utility lines, scanning for prey. They feed mainly on insects, especially grasshoppers and beetles; however, they may eat any animal they can overpower, including small mammals, birds, snakes, frogs and even, crayfish. They often hang their prey in tree or on barbed wire fences before eating it. This habit, unique among North American birds, has earned them in nickname, "butcher birds." They do this mainly to anchor their food in order to tear it apart but may also store food in this way for later use.
Shrikes require open land with lookout perches for hunting, preferring areas with short vegetation such as pastures, lawns and freshly-plowed fields. They seem to prefer sites with a variety of different types of land uses. They nest in dense, brushy vegetation, either in hedgerows or isolated trees, adjacent to feeding areas and usually on roadsides. Nearly half or all shrike nests found recently in Indiana have been in red cedar, but multiflora rose, sassafras and many other plant species are also used. The amount of cover provided is more important than the type of plant.
Shrikes build bulky, sturdy nests of stick and roots, well, lined with a variety of soft materials such as hair, feathers and cotton. They lay four to seven (usually five or six) speckled eggs which take about 17 days to hatch. The young leave the nest after about 19 days and may remain with the parents for up to a month while they acquire hunting skills. Mortality of young birds is very high during this period. Shrikes may raise two broods in a year. Nest sites are usually reused the following year if they have not been disturbed.
In presettlement times, when Indiana was largely forested, shrikes probably did not occur here. As the forests were cleared, shrikes expanded their range to occupy newly created habitat. By 1900, they were common throughout the state, especially in the north. Since World War II, however, changes in human demographic patterns and farming practices have eliminated much of the hedgerow and pastureland habitat that the shrikes require and they have declined over much of their range. Pesticides such as DDT may also have taken their toll. The loggerhead shrike is considered endangered in Indiana, and the northeastern subspecies is being considered for federally threatened status as well.
By the 1960s, the loggerhead shrike was believed to be nearly gone from Indiana. In the 1970s, very few were sighted and only one nest was found. Since 1980, however, sightings of shrikes have increased, and in the late 1980s, about 100 breeding pairs were located, mostly in southern Indiana. Most of these birds were in the southwestern counties of Davies, Dubios and Spencer. Many were found in Amish districts where more traditional farming practices have maintained suitable habitat. Some population remains in Indiana during the winter; however, the wintering grounds of the remaining population are unknown.
Although Indiana's shrike population is much larger than previously believed, its limited distribution makes it vulnerable. While the use of DDT is now banned, farming trends continue to cause the loss of shrike habitat. The key to conserving skrikes in Indiana is the maintenance of suitable nesting and feeding areas and the protection of known nest sites. Since nearly all shrikes occur on private land, efforts must be made to increase public awareness of the value of hedgerows to wildlife and appreciation of shrikes as valuable pest control agents. Road maintenance departments and utility companies can also play a role by avoiding disturbance of roadside nest sites. In addition, opportunities exist for the creation of shrike habitat on state-owned, reclaimed strip mines.


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Source: Dell
quote: Does anyone was really thinking that Windows 8 will bring people in drove to update their pc/laptops ? Windows 8 is such a radical move and bring nothing enticing to that platform. Why change your machine if W7 does everything you want just fine.
quote: If you have Win7-64, there's no reason to upgrade to Win8 Pro unless you have a touchscreen.
quote: XP was great for it's time. After SP2, you had a very stable product.
quote: XP was ****. Its a security risk and don't work with tons of new hardware.
quote: Name a piece of hardware that doesn't work with XP.
quote: Enterprise my friend.
quote: XP will have updates until 2014


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WWE Elimination Chamber emanates from Oakland, California in the Oracle Arena tonight. In preparation for the penultimate card leading up to Wrestlemania, all the last-minute updates, predictions and match-by-match analysis will come to you in the form of an all day live blog covering all things Chamber.
Follow Big Nasty on twitter at twitter.com/ThisIsNasty.


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Serves 4 |
Prep Time 1 hour 10 minutes |
Cook Time 10 minutes
1/2 cup peaches, sliced
1/2 cup blueberries, fresh or frozenor strawberries or raspberries
2 cups Eden Apple Juice
1 pinch Eden Sea Salt
1 bar Eden Agar Agar Barsor 2 T. Eden Agar Agar Flakes
Arrange the fruit in a mold or shallow bowl. Place juice, sea salt and agar agar in a saucepan. If using agar bars, break or cut into 1 inch pieces before adding to the juice. Bring to a boil, reduce flame to medium low and simmer 15 minutes for bars or 5 to 8 minutes for flakes. Stir occasionally until dissolved. Pour over the fruit. Let set in a cool place or chill in the refrigerator for 1 to 2 hours until firm.
Condiments, Dessert & Pastry, Eastern, Fruit, Gluten-Free, Japanese Food, Low Fat, Low Sodium, Macrobiotic, Sea Vegetables, Vegan, Wheat-Free


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Accommodations not included in the Rates above. Rates above include greens fees, cart fees, and taxes. Rates are per round.)


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Top Definition
A person who claims to not partake in the indulgence of any animal products, but still swallows cum. Like the term pescatarian, which combines the the Italian word for fish with vegetarian, Semetarian combines the the Italian word for semen with vegetarian.
I don't eat meat, or wear leather, but I do swallow jizz, therefore I am a Semetarian.
додав wpeterson 11 Березня 2011
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Can you define these popular missing words?


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How to Make Easy Sloppy Joes (01:55)
Sloppy Joes are often the target of sandwich snobbery—people just don't believe they're in the same class as clubs, Reubens, or even hamburgers. Which is a shame, because these hot, messy sandwiches are delicious. In this episode of The Easiest Way, Lisa Lavery of the CHOW Test Kitchen shows you how to make a simple, tasty version of this cafeteria classic using our easy Sloppy Joes recipe.
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The UHS Health Promotion Office emphasizes awareness of current health issues, prevention of disease and illness, and promotion of health and wellness. The primary goal of the Health Promotion Office is to provide opportunities for members of the University of Rochester community to develop health-promoting skills and attitudes necessary to make responsible decisions about personal health and health care.
The UHS Health Promotion Office is located on the second floor of the UHS Building (Rooms 225, 227, and 228) on the River Campus. The Health Promotion Office is open weekdays throughout the year.
For more information, contact Linda Dudman in the UHS Health Promotion Office at (585) 273-5770 or firstname.lastname@example.com.


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First comes the sound of wedding bells, and next comes the baby rattle.
Lady Antebellum's Hillary Scott and drummer Chris Tyrrell, who became engaged on Independence Day in 2011 and married Jan. 7 in upstate New York, are now expecting a child.
"Chris & I are excited to announce that our Christmas gift has arrived a little early this year! We are having a BABY!" she wrote on Twitter Friday. "We feel so blessed!!!"
"Everything is brighter. Everything has a deeper meaning," Scott, 26, previously said of her new husband. "He makes me feel more comfortable in my own skin."
Scott has certainly had kids on the brain: On Monday, the band announced they are starting a new charity called LadyAID, which will focus on children's hospitals and as well as other charitable causes.
Scott and Tyrrell met while they were in college, though things didn't turn romantic until later. Lady Antebellum's song, "Just A Kiss," was inspired by Scott and Tyrrell's relationship when they first began dating.
Lady Antebellum's Hillary Scott Is Pregnant
Courtesy Hillary Scott


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The Brookings Public Library Board recently accepted the report and plan submitted by the 2013 Citizens' Strategic Planning Committee. This plan will direct the Library's continued reach for excellence through 2016.
The Committee recommended three roles on which the Library should focus:
- Lifelong Learning
- Reading, Viewing, and Listening for Pleasure
- Creating Young Readers through Early Literacy
To see the complete report and the objectives planned to achieve these goals, please go to the "How do I . . ." tab at the top of this page. The report is also available for review at the Library Circulation Desk.


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Bonham wins debut as Columbian coach
It was a successful debut on the bench for Bill Bonham at Columbian. The Tornadoes hosted Hopewell-Loudon and came away with a 25-19, 25-16, 25-9 victory.
Alyssa Price helped out TC all over, recording 13 assists, four aces, 10 kills, four blocks, four digs.
Haley Sendelbach pounded 15 kills and eight blocks, Tessa Fuhrer tallied 16 assists and four aces, Taylor Reinbolt turned in 11 digs and four kills and Maddie Williams had five kills, three aces and six digs.
Hopewell-Loudon was led by Courtney Burns' double-double. The senior slammed 10 kills while also dishing out 10 assists. She also added five digs and three blocks.
Kassie Shaver had five kills and five digs while Kassie Welly had 10 assists and five digs. Baleigh Searles led the defense with 10 digs.
Ladd leads NR?triumph
NEW RIEGEL - It was the season-opener, but New Riegel already looked in mid-season form Saturday, dominating visiting Margaretta 25-9, 25-8, 25-16.
Lauren Ladd led the charge with 19 kills while also dishing out six assists. Taylor Arbogast chipped in 10 kills. Morgan Noftz handed out 29 assists while Natalie Reinhart served three aces.
Defensively, Brittany Gillig had 18 digs and Kara Scherger had 16.
Devilish domination
CAREY - If you add up Vanlue's scores for its entire match against Carey, it still wouldn't have been enough to win a game. Carey dominated the Wildcats to open up the season 25-6, 25-3, 25-7.
The Blue Devils had 21 team aces, seven of which came from Mackenzie Wentling and five from Sydney Wentling. Sydney Wentling also added 19 assists.
Shelby Kinn slammed down eight kills and added three blocks. Shelby Seifert chipped in five kills.


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Hemorrhoids Quiz: Test Your Medical IQ of Piles
Reviewed by Jay W. Marks, MD on Septemeber 16, 2011
Images provided by:
1. iStockphoto
2. iStockphoto
3. iStockphoto
4. Bigstock Photo
5.1 iStockphoto
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6. MedicineNet.com
7. iStockphoto
8. iStockphoto
MedicineNet. Hemorrhoids.
<http://www.medicinenet.com/hemorrhoids/article.htm >
WebMD Medical Reference from Healthwise. Hemorrhoids – What Happens?
WebMD Medical Reference from Healthwise. Topic Overview – Hemorrhoids.
This tool does not provide medical advice. See additional information:
THIS TOOL DOES NOT PROVIDE MEDICAL ADVICE. It is intended for general informational purposes only and does not address individual circumstances. It is not a substitute for professional medical advice, diagnosis or treatment and should not be relied on to make decisions about your health. Never ignore professional medical advice in seeking treatment because of something you have read on the MedicineNet Site. If you think you may have a medical emergency, immediately call your doctor or dial 911.
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Expanding Use of NGS Has Largely...
LC-MS Gains Favor Due To the...
Developers of DTC Genetic Testing Are...
As Immunotherapy Revolutionizes the Cancer Treatment...
Researchers have identified a gene that, when repressed in tumor cells, puts a halt to cell growth and a range of processes needed for tumors to enlarge and spread to distant sites. The Johns Hopkins researchers hope that this "master regulator" gene may be the key to developing a new treatment for tumors resistant to current drugs.
"This master regulator is normally turned off in adult cells, but it is very active during embryonic development and in all highly aggressive tumors studied to date," says Linda Resar, M.D., an associate professor of medicine, oncology, and pediatrics, and affiliate in the Institute for Cell Engineering at the Johns Hopkins University School of Medicine. "Our work shows for the first time that switching this gene off in aggressive cancer cells dramatically changes their appearance and behavior."
Dr. Resar has been investigating genes in the master regulator's family, known as high-mobility group (HMG) genes, for two decades. In addition to their role in cancer, these genes are essential for giving stem cells their special characteristics. "Many investigators consider cancer cells to be the evil twin of stem cells, because like stem cells, cancer cells must acquire special properties to enable the tumor to grow and metastasize or spread to different sites," she explains.
She and her team previously devised techniques to block the HMGA1 gene in stem cells in order to study its role in those cells. In their prior work, they discovered that HMGA1 is essential for reprogramming adult cells, like blood or skin cells, into stem cells that share most or all properties of embryonic stem cells.
In the new study, Dr. Resar's team applied the same techniques to several strains of human breast cancer cells in the laboratory, including triple negative cells—those that lack hormone receptors or HER2 gene amplification. Triple-negative breast cancer cells tend to behave aggressively and do not respond to many of the most effective breast cancer therapies. Dr. Resar's team blocked HMGA1 expression in aggressive breast cancer cells and followed their appearance and growth patterns.
"The aggressive breast cancer cells grow rapidly and normally appear spindle-shaped or thin and elongated. Remarkably, within a few days of blocking HMGA1 expression, they appeared rounder and much more like normal breast cells growing in culture," says Dr. Resar. The team also found that the cells with suppressed HMGA1 grow very slowly and fail to migrate or invade new territory like their HMGA1-expressing counterparts.
The scientists then implanted tumor cells into mice to see how the cells would behave. The tumors with HMGA1 grew and spread to other areas, such as the lungs, while those with blocked HMGA1 did not grow well in the breast tissue or spread to distant sites.
"From previous work, we know that HMGA1 turns on many different genes needed during very early development, but it's normally turned off by the time we're born," says postdoctoral fellow Sandeep Shah, Ph.D., who led the study. "Flipping that master regulator back on seems to be necessary for a cancer to become highly aggressive, and now we've seen that flipping HMGA1 off again can reverse that aggressive behavior."
The next step, Dr. Resar says, is to try to develop a therapy based on that principle. The team is working with other researchers at Johns Hopkins to see whether HMGA1-blocking molecules could be delivered to tumors inside nanoparticles. Another possible approach, she says, would be to block not HMGA1 itself, but one of the pathways or processes that it affects.
The study appears in the May 2 issue of the journal PLOS ONE. The paper is titled "HMGA1: A Master Regulator of Tumor Progression in Triple-Negative Breast Cancer Cells".
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The most awesome Turkish name you can ever find. If you have seen this name stop running away and ask for an autograph.
Girl 1: Have you seen that Turkish boy over there? Girl 2: Yeah.. I think that's Ege, he's so cool.
viết bởi IhawtUnawt 21 Tháng bảy, 2009
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Can you define these popular missing words?
1) The definition of an alpha male.
2) Name of a sea between Turkey and Greece
2) Name of a sea between Turkey and Greece
"All guys out.. Ege in"
viết bởi Skaterguy98 06 Tháng mười một, 2008
I'm sorry this is not a real school. Please search a school that actually exists
ege: I didn't get into NYU so i had to attend Queens Coll
viết bởi Charlie Brownies 02 Tháng một, 2014


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Gov. Tomblin signs education reform bill
April 11, 2013
CHARLESTON — Stakeholders in West Virginia's education system and lawmakers alike made no issue in calling Go....
Tiffany Lawrence is a vapid moron former beauty queen who is in NO WAY qualified to represent anyone. But you idiots voted for her so that's what we get.
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No-one in this photo looks very happy! Is it because they know that this bill is watered down to the point that it won't do much to really help the situation? More days in the classroom watching Disney videos and more days in the classroom with underpaid, unqualified teachers won't fix our problems. Our schools need quality (quality days of instruction and quality instructors) not just quantity. We need to hire and pay for real quality teachers and stop paying for so many aides and administrators! You get what you pay for!
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This is rather like the Manchin compromise in which the politicians slap one another on the back in congratulatory fashion. "Yeah! We did something!" In actuality, of course, all that they accomplished was to provide some campaign material for their next election. I mean, look at the quote from Lawrence. Does she even know what she is trying to say?
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on October 14, 2012 - 8:08 PM
, updated October 15, 2012 at 6:06 PM
GLENDALE, Ariz. – For the Buffalo Bills on Sunday, it was Operation: Save the Season.
Consider it mission accomplished. Barely.
The Bills' wild, 19-16, overtime victory over the Arizona Cardinals at University of Phoenix Stadium salvaged a split of their two-game West Coast trip and, more importantly, helped them leave behind two straight historically inept defensive performances.
"I am excited for those guys who have worked their rears off," Bills coach Chan Gailey said. "They lost some respect the last couple of weeks, and they were out to gain some respect back. I think they did that."
A game that could be described as somewhat dull for the first 56 minutes had more ups and downs than an elevator in the final four.
The Bills watched as Cardinals kicker Jay Feely booted a career-long and franchise-record 61-yard field goal with 74 seconds left to tie the game, then had to hold their breath when – after the offense went three-and-out – Arizona got in position for a 38-yard field goal try at the end of regulation.
That kick, however, was partially blocked by defensive tackle Alex Carrington, who got just enough of it to force it off the left upright.
"I couldn't believe it at first," Carrington said. "I thought it was still going to go in because the ball just kept going. Me, Marcell [Dareus], Shepp [linebacker Kelvin Sheppard], we were able to get under their pads. I was able to get a hand up and got the ball. It was crazy. We just tried to give it all we had. The game was on the line, we had to do something."
"You can't explain the emotions. There is just no way," Gailey said. "You don't think there is any way a guy is going to make a 61-yarder, and then you think for sure he is going to make the short one and he doesn't. There are highs and lows. As football teams, you have to handle highs and lows not only in a game but in a season. You just keep fighting."
The Bills won the toss in overtime and drove to the Cardinals' 35-yard line. They elected not to try a 53-yard field goal with Rian Lindell, however, when the drive stalled, instead punting the ball away.
The decision worked.
Free safety Jairus Byrd intercepted Cardinals quarterback John Skelton – who replaced the injured Kevin Kolb late in the fourth quarter – on a third-and-10 play and returned the ball 29 yards to the Arizona 6.
Lindell knocked in the game-winner, a 25-yard field goal, two plays later.
"It's huge, but it's not relief," Gailey said.
"It's excitement about where our team can go. This was a tough football game, and it was against a good football team. To come out on the road and win it the way we did, I am impressed with the way the team played."
The Bills looked to be in good position to win in regulation, until an ill-timed Wildcat play call.
Leading by 16-13 at the Cardinals' 36 with four minutes left, Brad Smith threw from the formation for the first time this season. The ball, severely overthrown for Donald Jones, was intercepted by Patrick Peterson in the Cardinals' end zone.
"We were trying to throw it more in the middle of the field to keep it away from the outside. We designed it to go to the middle of the field, but it ended up not hitting the middle of the field. If we hit that, we are all talking about what a great call it is, and if we don't, it's a dumb call," Gailey said.
"So it was a dumb call. If I had to do it over again, I'd run it."
The Cardinals drove 10 plays to the Buffalo 43 to set up Feely's bomb despite losing Kolb, who was injured with 2:06 left to play when he scrambled right and was brought down by Chris Kelsay and Carrington.
The Bills sacked Kolb five times – maligned defensive end Mario Williams had two of those – and hit him several others.
"As early as Monday when we got in from San Francisco we started watching film and to put it bluntly, we knew they must have had a really good defense," said Kelsay, whose first-quarter sack of Kolb resulted in a safety for Buffalo's first points.
"Up front, if they're able to throw on time, they're going to make plays. They've got the receivers to do that obviously but we knew that [if] we could just turn it loose, play fast and get pressure on the quarterback we should fare pretty well, and that was the case [Sunday]."
It was a hop, skip and a jump in the right direction for a defense that had given up 90 points in its last six quarters.
"When we go back and watch the film, yes those losses are ugly, but when we watched the film, there was nothing that was not correctable," linebacker Bryan Scott said. "When you get those things corrected you can see what the results will be."
The Bills, who got 88 yards on just 12 carries from running back C.J. Spiller, won despite an uneven performance from quarterback Ryan Fitzpatrick. He went just 18 of 32 for 153 yards, with no touchdowns and no interceptions.
A Picasso this was not.
"It wasn't pretty at times, but it was a gut check and that may got a long way for our football time," Gailey said.
The win evened the Bills' record at 3-3, pulling them into a four-way tie for first in the AFC East.
"We came out here with one goal, and that was to win," Fitzpatrick said. "In the NFL you celebrate those victories, because we know they're tough to come by. We put a lot of work in this week. In general, our whole organization put a lot of work into this week. This was a very satisfying win for us."
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The sixteenth full length Redwall novel sheds light on the Abbey's ancient origins in a thrilling adventure. Loamhedge, the deserted Abbey, has been forgotten for countless seasons. What secrets do it's ruins hold? When it becomes clear that wheelchair-bound Martha might be cured by a formula buried there, two old warriors are inspired by the spirit of Martin the Warrior himself to go on a quest for the ancient Abbey and three young rebels are determined to go with them. Meanwhile. the giant badger Lonna Bowstripe thirsts for vengeance as he relentlessly pursues Raga Bl and his murdering crew of Searats…who are on their way to attack Redwall itself. The valiant Abbeybeasts must defend their home, but how can they, when their boldest warriors are away on their quest? Will Redwall fall to vermin invaders at last?
"Redwall fans will like this latest adventure from the master of medieval fantasy."—Detroit Free Press
"This midsummer adventure…will not disappoint."—Horn Book Magazine
"A sophisticated read, filled with larger-than-life characters…and all kinds of humor."—School Library Journal
"Action-packed adventure. The story is laced with humor, the feasts are mouth-watering, and the language is rich."—Booklist


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