text:
Printable Recipe Here
Sunday, February 20, 2011
Famous Potato Salad
Printable Recipe Here
88 comments :
Brandie, this sounds and looks great! I would have never thought of adding chicken broth. Mom's always DO know what to get!ReplyDelete
What a sweet memory! Moms are such a blessing. How long did it take you to get used to driving on the "wrong" side? :)ReplyDelete
Sydney - it took me a good 2 months to get completely comfortable. I cannot even tell you how many times I would go to work early in the morning (half awake) and accidentally start off on the right side of the road and then quickly have to get back over to the left side. It is so second nature! Thank the Lord I never got in an accident!ReplyDelete
Hehe, well that is shorter than I would have guessed for myself to get used to it! What job did you find there? I imagine it might have been difficult finding one without England residency? I don't know...ReplyDelete
I love your memories of the cookbook and moving to England! I would have been totally freaked out moving that far away!ReplyDelete
I worked in banking. I had a really great job with some pretty fantastic people. They helped me learn so much. And I'm fortunate that we are all still friends after all these years! I highly recommend traveling overseas (if you haven't done so already). It is so much fun to be young and travel. Opens you up to some wonderful experiences!ReplyDelete
That potato salad would go well with a barbecue sandwich. If I close my eyes, its summertime.ReplyDelete
Wow, that's great! We recently moved back from living in Peru for a long time [so I also recommend the same thing - love it!] and it was extremely difficult for a non-resident without various other approvals to get any sort of job! What a blessing you had special friends help take care of you in your hugely new experience!ReplyDelete
Sydney - I definitely think Peru would be far more of a culture shock! What an experience that must have been for you! I just think it is amazing that you took the initiative to do something like that. I would love to hear more about your travels!ReplyDelete
What a sweet memory..love your writing! And potato salad looks so tasty...one of my favorites! Yummy photo too!ReplyDelete
You have such a sweet mom(not that it's any surprise!). This looks like the classic potato salad that I love...now I'm getting antsy for summer so I can make some to go with some grilled burgers. YUM!ReplyDelete
Awwww, that brought a tear to my eye when reading about your Mom and her gift to you. And what an amazing experience to live overseas, newly married. Your potato salad sure looks good!ReplyDelete
That first cookbook of yours is just so precious. It's extra special with your Mum's gorgeous comment in it, too! I bet you know them all from memory by now ;-) That was so young to cope with such a huge change. You're amazing!
This potato salad sounds like the kind that my Mom used to make except for the chicken broth. Very sweet of your Mom to give you the cookbook. Whenever there was a get together I would always have her bring her potato salad, because I didn't like to peel potatoes. I was young then.ReplyDelete
Awww, what a neat story. And, I know from reading your blog regularly that you've turned into an excellent cook!! We'll be trying this potato salad soon.ReplyDelete
Great story! I, too, have many dishes I treasure from my first Betty Crocker -- only mine was not a big book like yours. It was a "Dinner for Two." (Just as well-used, though!)ReplyDelete
Brandie-I loved reading your story, it was so heartwarming to hear about your life. Aren't moms the greatest?ReplyDelete
This is my very favorite potato salad, that I also make. Can't go wrong with Betty Crocker.
Your potato salad looks so yummy, and fluffy. Such a classic recipe!
Thanks for sharing your sweet story and recipe!
...and now I am totally craving THIS potato salad! Looks fabulous :) I am definately bookmarking this!!ReplyDelete
Moms are the best and we truly begin to appreciate them when we ourselfs become one! What a wonderful post and potato salad looks yummy and sounds delicious! Thank you for sharing :)ReplyDelete
What a great post Brandie, Moms and Grandmas are always the best. They'll always make us see the light an the the shoulder to cry on.ReplyDelete
The potato salad looks delicious. Looking forward to the cheesecake recipe too :)
Hope you'll have a wonderful Monday
What wonderful memories you have of learning to cook! Thank you for sharing the story, especially about the start with the fruit soup. We all have to start somewhere and it's so lovely that your colleagues were so helpful. Thanks for sharing a great and classic recipe with me, too!ReplyDelete
What a unique recipe using chicken broth. I love seeing new ways to create classics.ReplyDelete
Your potato salad reminds me of my first days of blogging. I made a potato salad that sounds identical to yours. Delicious! I had to laugh with your fruit soup story. I had cooking anxiety when we got invited to my guy's supervisor's house for Thanksgiving and I was asked to bring dessert. I set out to testing recipes the instant I got my assignment. Even after making the recipe a handful of times my nerves were still racked that day. My dessert ended up in the pantry somehow at their house never to even be set out. Needless to say I was too embarassed to even ask for my tupperware container back. Maybe I'll get my reprieve like you got.ReplyDelete
This potato salad looks great. How sweet of your momm to send you the cookbook :) My mom gave me a binder of family recipes that she made especially for my one year at christmas and it is so special :) Also, thanks for the tip on not twisting the biscuit cutter. Have a nice day!ReplyDelete
Hi, Brandie! Thanks for stopping by my blog and leaving such a sweet comment :) Thank goodness for mom's, right? What a great story behind this dish - a huge transition for someone so young. We've visited London a few years ago, but I don't think I'd be brave enough to drive there! We did drive in Ireland, but at least they were mostly small towns and country roads! Your Country potato salad is as good as it gets - and its great you added the boiled egg tips and no substituting Miracle Whip!!!ReplyDelete
Such a great memory attached to that book! It looks very well-loved over the years. The potato salad looks delish, perfect for upcoming spring!ReplyDelete
I love potato salad! This recipe looks delicious!!ReplyDelete
I so enjoyed hearing about your newlywed days (in a new country on top of that!) and the sweet gift that your mom shared with you. Moms do know best! Thank you for sharing your words and this recipe! And thank you for your sweet thoughts on my blog. I appreciate you!ReplyDelete
What would we do without our mothers? Sweet story! You're killing me with all this delicious summer food, though, first the baked beans, now this delicious potato salad. I may have to break down and have a picnic in front of the fire place!ReplyDelete
Mothers are truly the sweetest :)ReplyDelete
One of my first cookbook was a betty crocker one too amd i still use it...hehe.
Your salad looks delicious with all that potato goodness...drool!
Moms are often so inspired. That is a great looking potato salad. I can see why it is still your favorite.ReplyDelete
Thanks for coming by. I loved this story and the potato salad looks wonderful. All your pictures look great, too. Some wonderful recipes here!ReplyDelete
Great story. First cookbooks always have such great memories baked into them!ReplyDelete
Cute story! Most of my cookbooks pages look like that too : )ReplyDelete
I love potato salad and this just makes me crave summer!!! Can't wait to make it and bty that cook book looks very similar to my mama's!!ReplyDelete
What a great story! Betty never lets you down!ReplyDelete
How very cool is this story! I still have the first cookbook my mom gave me, though mine's Better Homes and Gardens. The one with the 3-ring binder.ReplyDelete
I LOVE this potato salad. It looks so very creamy and delicious.
Aw, so sweet. My first cookbook was Better Homes and Gardens, back in university. That thing was so beat up and dog-eared by the time I was done with it! Great potato salad.ReplyDelete
FYI, erythritol can be purchased online or at Whole Foods (Zsweet brand). Is your mum a diabetic? If she's just gluten free, use real sugar (about 1/2 cup). It's cheaper!
what a precious gift! and a great recipe! I just wanted to let you know that I am loving your blog so I left you a Stylish Blogger award on my blog.ReplyDelete
Hello there deary! You have been tagged! http://ravienomnoms.wordpress.com/2011/02/23/pretty-pepper-pasta/ReplyDelete
I'm a little behind on my reads lately, but I just had to comment on this before I hit the cheesecake post. This story sure brings back memories of my first years in marriage. My mom bought me a similar cookbook that I still like to cook out of. Only now, I'm more free to add and take away ingredients and feel completely confidant. Thanks for sharing this story Brandie:)ReplyDelete
I loved your story. I'm so glad I found your blog today. I came over from What's Cooking Wednesday! This potato salad looks delicious and makes me think of summer barbecues. :)ReplyDelete
I just LOVE potato salad. Your recipe looks delicious!ReplyDelete
I just love hearing about your early years and your family...really good stuff. The Potato dish looks so delicious, we just love dishes like this. Thank you so much for sharing these precious moments with us at Full Plate Thursday and please come back!ReplyDelete
Love the recipe and the sentimental connection :)ReplyDelete
This looks wonderful! I have been having a blast looking through all of your tasty recipes! My mom got me a cookbook the first Christmas I was married and boy has it been a lifesaver sometimes! :)ReplyDelete
I'm off to the kitchen to make some potato salad now. :)
Heh, I know what I'm bringing to easter dinnerReplyDelete
I love potato salad!ReplyDelete
I'm hosting my first blog hop over at Peas & Carrots if you're interested in linking up. It's at www.navefamilyfood.blogspot.com
YUM, I love me some good Potato Salad!ReplyDelete
Would love for you to share your recipes on "Made it on Monday"
*link ups are every WED.-SAT.*
Hi there! Don't you just love classic cookbooks! My mom has a Betty Crocker book from the 1950s and it is just a treasure trove of classic recipes that we still use today! Great post!ReplyDelete
Your potato salad looks heavenly! I bet the chicken stock adds a ton of flavor!ReplyDelete
I've been looking for a potato salad recipe and this one looks yummy! I must admit though that I am a Miracle Whip girl ;)ReplyDeleteReplies
I'm a mayo person,husband a Miracle Whip guy,so I use a combination of both A better flavour,I think.Delete
- AnonymousAugust 14, 2013 at 12:20 PM
Yes, I am a Miracle Whip person too, and the potato salad recipe is different, with the chicken stock, but I also add a couple packets of sweetener to off set the tanginess of the mustard.Delete
- AnonymousMay 28, 2011 at 5:40 AM
I like this site^-^ReplyDelete
- TinalouJune 1, 2011 at 4:56 PM
I love trying new recipes and need a good potato salad recipe for the summer - this one looks like a winner. Hubby isn't fond of mustard - how do you think it'll taste without the mustard??ReplyDelete
Tina - thanks for the comment! The mustard is not a strong flavor in it at all. It really is more in the background. Also, you could try dijon instead of yellow which has a milder mustard flavor.ReplyDelete
Potato salad is one of our all time family favorites! I've made some with only vinegar and some with only mustard, but never the two combined. Sounds like a yummy idea!ReplyDelete
Newly following from the Wednesday hop. I'd love to have you stop by and say hello!
Mrs B
Great potato salad recipe!! Yum!! Looks yummy!ReplyDelete
Looks wonderful! Oh those memories of our young married days can bring some good laughs huh? I have those too. Thanks for sharing yours...and your treasured cookbook. Great post Gal! Enjoy a wonderful weekend...I've already linked some favorites to Weekend Potluck. Thanks for all you do to make my life brighter! XOReplyDelete
My Mom gave me my Grandma's Betty Crocker cookbook when I left home back in 1980. It was dated 1956. I treasure that book and seldom bring it out these days. It hangs on my dining room wall in A special rack that also holds a rolling pin. I will hand it down whwn my sons marry.ReplyDelete
Love the potato salad recipe. It is pure southern style, although I do love Miracle Whip in it too.
I also boil my eggs the way you do. They turn out perfect every single time. Creamy and no green!
Love your blog. Keep up the good work!
Teresa - I love hearing that! Such special memories!ReplyDelete
OH my another great hit..girl you got it goin on, everything we've tried from you has been great!! I have been looking & looking for my moms old potato salad recipe that was given to her mother & so on & so forth, but unfortunately with a passing of family members things go "missing" :( Sooo for the most part from what I could remember from helping her so much when I was younger I knew some but couldn't remember exact measurements & so on. One thing I have never heard of was using chicken broth/stock in potato salad but O-M-G this is that "secret" ingredient that you wonder what it is that makes it taste soo great. Of course as we all do I put my own twist on this--i'm not a big celery person so instead of celery I used relish & for this "sauce" it was a little too much but thankfully I had made a WHOLE bunch of potatoes so it worked out well adding a little bit more potatoes, onions, relish, & eggs. Though it's not my moms it's a nice "my" modern twist of hers....thank you Brandie sooooo muchReplyDelete
I made this patatoe salad yesterday for my bday party and let me just tell you my guest loved it to the point were my bowl was left licked clean lol very creamy I added crushed bacon gave it a more zing to it ty so much for this recipeReplyDelete
- AnonymousJanuary 6, 2013 at 10:02 PM
Just found your website...absolutely LOVE itReplyDelete
This recipe is awesome!I could've eaten all myself! ;)ReplyDelete
This one is definitely going in my "keep" file.
Yay!! I am so happy you had success this one! Thank you so much for letting me know how it turned out for you! I am so happy you loved it as much as we do. I hope you find lots more recipes on here that you love!ReplyDelete
I have that same Betty Crocker cookbook. It is the best cookbook I own--everything you need know about how to cook, from boiling an egg to making homemade gravy. Great gift for a bridal shower or a bachelor. I really enjoy your blog. Your recipes are fantastic. Thank you for sharing them.ReplyDeleteReplies
I will keep it forever even though it is falling apart! :) ANd I love to give it as a gift to younger folks who are just moving out on their own.Delete
This potato salad recipe is the same as my mom's/grandma's, and it's perfect. Not too mustard-y, not too bland, just ... perfect! Now about that cheesecake recipe ... ? ;)ReplyDeleteReplies
Exactly! I know some folks see the eggs and mustard and wonder if it is good but I tell them that I promise it all blends well. Not too strong mustard or eggs. Thank you for taking the time to comment! :)Delete
thanks for the egg boiling tipReplyDelete
I just made the potato salad, it was really good and so easy, my family loved it. I need to make it again to get more for myself this time. As for the eggs... I bought them already hard boiled and peeled... I know its kinda lazy but its faster.
Made the potato salad Sunday. Love, love, love it! My husband is a picky eater and his mom always brings potato salad to our summer get-togethers. He said his mom's feelings are going to be hurt because he won't eat any other potato salad but this one!ReplyDelete
I love this potato salad! It is my most favorite EVER! Thank you for sharing the recipe.ReplyDelete
I love your story!ReplyDelete
Potato Salad is one of my all-time favorites!
Brandie, I pinned this the last time I saw it. It sounds so much like my Mom's. She didn't use the broth and she added sweet pickle cubes and some of the juice.ReplyDelete
I know that cookbook and the message she hand wrote to you is something you will always cherish!!
I enjoy your stories and your recipes!!
- AnonymousMay 26, 2014 at 11:06 AM
I love your story! What kind of onion do you recommend? I made this using less onions than recommended in the recipe, and by the next day it tasted completely like onions :-(ReplyDelete
Anonymous - oh no! You could use a sweet onion next time (like Vidalia) or you could use shallots which are a milder onion flavor. Hope that helps! :)ReplyDelete
Thank you for sharing your recipe and making the steps easy for all to understand. I have added a link to your page from my blog. I do hope that is ok. If not please let me know and I will remove it. http://dailydiylife.blogspot.comReplyDelete
Thank you!
- AnonymousJune 23, 2014 at 3:46 PM
Hi! I have made so many of your recipes and some of them are my favorites! I actually just made one this AM. Anyway, I HAD to comment because I actually HAVE that cookbook! My grandmother gave it to me when I was 19. I wanted to tell you that if you have not made the Pancake recipe, you have to try it. It is the best recipe I have come across.ReplyDelete
- AnonymousJuly 2, 2014 at 2:19 PM
This sounds so yummy! I am wanting to make this this weekend, but I have a quick question. About how many people would you say this will serve?ReplyDelete
Cheesecake recipe, pleaseReplyDelete
Hi Brandie, I was just on pintrest looking at potato salads. My all time favorite is mayo, mustard, eggs, celery and onion. Never thot about chx broth. You know what i do use is dill pickle juice. Perfect replacement for vinegar, No? Try it next time. What do people do with all that juice anyway? lol Teri.ReplyDelete
I made this salad today because I was looking for a basic recipe since I didn't want to run to the store and knew I would have everything for something basic. I didn't use the chicken stock because I didn't feel like making any this morning. I also added a spicy mustard along with the yellow mustard and used white wine vinegar instead of plain. I also used a red onion for color. This turned out great! And btw, that cookbook was my first cookbook too! I used that all the time when I first started cooking because it had so much info! If you're a first time cook that's definitely the book to get.ReplyDelete
Yummy looking potato salad. When I spent a semester abroad while in college, our England base was in Northwood at London Bible College. Loved that little village!ReplyDelete
- AnonymousJuly 12, 2015 at 5:14 PM
Do you let the potatoes cool or mix them in hot?ReplyDelete


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As in to Ford someone, mainly a male. To give a male a blow job. Normaly, one Fords a male they have just met, or while intoxicated.
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af goldfishOHYEAH 17. januar 2011
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To be an alcoholic. or to just get drunk or be drunk.
Lets get Forded tonight.
af LilTunechi90 13. april 2011

subset: commoncrawl


text:
Top Definition
When you finally find an empty public restroom for a dump so massive that it requires complete solitude for the deposit....and then someone walks in.(especially at your office/work place)
Just as I was about to 'release the beast', some turd buglar entered the bathroom causing my sphincter to snap shut!
by Sidney Abello February 11, 2004
Trending Now
Can you define these popular missing words?
In Quebec, the thief who follows the first burglar and the second burglar.
We arrested de first two burglars, but de turd burglar got away.
by Saddam Hussein May 09, 2003
yet another word for gay men
jeez joe is a turd burglar!!
by turd-bur-gal-er April 24, 2003
One who burgles turds
My cat took a crap and the dog ate it...he sure is a turd burglar
by baldylocks1976 January 29, 2005
(UK) Slang for homosexual.
"Hey, turd burglar! Get off that guy!"
by - August 04, 2002
The Hamburglar's more fetid and vastly less successful predecessor.
I thought the McCrappy Meal cam with a Turd Burglar toy...
by AngryChegro July 10, 2003
A derogatory term for a man of homosexual tendencies.
See also: Marmite/Vegemite/Chutney Ferret; Tail-gunner; and obviously faggot etc.
Man that guy is a turd-burglar
by Anonymous October 29, 2003


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on December 6, 2012 - 12:38 PM
, updated December 6, 2012 at 12:39 PM
Spirit Airlines has moved up the start date for its 2013 seasonal service between Niagara Falls International Airport and Myrtle Beach, S.C., and will bolster its service to Fort Lauderdale, Fla.
Two weekly nonstop flights between Niagara Falls-Myrtle Beach service will begin Feb. 15, nearly two months earlier than in the previous two years. Its service will increase to four weekly flights on April 25.
Spirit is moving up the start date for service to Myrtle Beach at a total of five airports in its system.
Additionally, from Jan. 14 to April 25, Spirit will operate daily service between Niagara Falls and Fort Lauderdale, instead of three days a week, Spirit said.


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Indicates the manufacturer of this product is a member of the Roundtable on Sustainable Palm Oil (RSPO). Planting of plantations of palm oil, which may be in as many as 1 in 10 cosmetics products, can result in significant deforestation of tropical rainforests and threatens the survival of endangered species such as the orangutan and Sumatran tiger. The RSPO, which was established in 2001, is committed to promoting the growth and use of sustainable palm oil through co-operation within the supply chain and open dialogue with its stakeholders. To learn more, visit www.rspo.org.
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The Stetson men's basketball team will play three games in three days as a part of the inaugural Gulf Coast Showcase beginning Monday in Estero, Fla. The Hatters take on Wagner Monday evening at 6:00 p.m. in the first round of the eight-team tournament.
Stetson (0-5), in search of its first victory of the season, has improved steadily over its five road contests to start the year. On Friday the Hatters led by as many as six points in the second half and were tied with still undefeated USF with just over four minutes remaining in a 72-63 defeat.
Veterans Willie Green and Aaron Graham led the Hatters in scoring for the second straight game on Friday. Green poured in a career high 20 points on a blistering 9-of-13 shooting from the floor, while Graham added 15 points on an efficient 3-of-4 shooting night from beyond the arc.
Graham is leading the Hatters with 12.2 points per game through the first five tilts, and has recorded multiple three-pointers in every game. Already with 11 triples on the season, the senior guard is now just four threes away from breaking the Stetson all-time record for career three-pointers. His 177 treys trail only former teammate Joel Naburgs, who tallied 180 in his four seasons in DeLand.
Stetson will open up the tournament against Wagner (3-2) at 6:00 p.m. from Germain Arena in Estero. The Hatters and the Seahawks have never met in the storied histories of both programs, which combine for over 200 seasons of basketball.
It will be common for Stetson to meet an unknown foe this week, as the Hatters have only ever squared off against one other team in the tournament. Stetson is 2-3 all-time against Southern Illinois, a possible opponent for Stetson on Tuesday.
The Hatters will play either SIU or St. Bonaventure in the second round of the Gulf Coast Showcase, scheduled for either 2:30 p.m. or 8:30 p.m. on Tuesday, depending on the outcomes of Monday's games. Wednesday will be the final day of competition, when Stetson could take on any of the four teams on the other side of the bracket (Louisiana Tech, San Diego, Illinois-Chicago, UNC Greensboro).
All of Stetson's games at the tournament will be aired live on the Hatters Radio Network with Robbie Aaron on the call. Fans can keep up with all the action at the Gulf Coast Showcase by following @StetsonMBB on Twitter and Instagram. UPDATE: There will be a live video stream (subscription) for all this week's games on America One Sports.


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text:
Picture tells a thousand words
Social Situations
General Gun Discussions
> Picture tells a thousand words
April 17, 2013, 10:22 PM
If you enjoyed reading about "Picture tells a thousand words" here in TheHighRoad.org archive, you'll LOVE our community. Come join
today for the full version!
April 17, 2013, 10:32 PM
Ha,ha,ha,ha!!!! Roflmao!
M2 Carbine
April 17, 2013, 10:32 PM
I love it.:D
April 17, 2013, 10:36 PM
Who is the doofus behind Gabby (and why is she even there to begin with)? He looks about ready to cry. Or is he filling in for Biden's 'I have to fart' look because Biden is face palming?
April 17, 2013, 10:39 PM
Should be the next cover of NRA magazine.....
April 17, 2013, 10:39 PM
April 17, 2013, 10:40 PM
I'm so happy right now I think I am going to take my double barrel shotgun out on the back porch and shoot both barrels in the air! :D
April 17, 2013, 10:42 PM
Wrong terms there TIMC..I think you meant fire two "blasts"..what are barrels? The things that go up?
April 17, 2013, 10:45 PM
Wrong terms there TIMC..I think you meant fire two "blasts"..what are barrels? The things that go up?
Sorry, my bad; please forgive me, it was my first try ad being a democrat and I'm not very good at being stupid yet. :neener:
April 17, 2013, 10:47 PM
Don't forget the clip for your double barrel.
April 17, 2013, 10:49 PM
Here ya go Coop :D
April 17, 2013, 10:52 PM
Someplace in Texas, George Bush, is laughing his arse off & Obama is chasing Biden around, Washington, with a tail to pin on him. He thought this was a free throw.
That's what you get when you mess with a few Bible toting Rednecks.
April 17, 2013, 11:05 PM
"Who is the doofus behind Gabby (and why is she even there to begin with)? He looks about ready to cry."
His son was killed at Sandy Hook. I don't begrudge the parents. They are being manipulated by the antis, Yobama and Buckshot Biden. maybe the truth and reason are comeing out. Glad this got stopped. Hope they can repeal the NY Safe BS.
April 18, 2013, 12:11 AM
Biden was standing there with his arms crossed and a scowl on his face, he looked exactly like Jeff Dunham's Walter. Made me laugh when I saw it. I felt sorry for Gabby, standing there nodding like a bobblehead doll. No disrespect intended.
April 18, 2013, 12:18 AM
Why does it make me so happy to see Biden's grief? LOL, what an ass.
April 18, 2013, 12:25 AM
I just thought it was ironic that right after the vote on Toomey/Manchin Biden was telling someone else to shut up, although he was using proper Senate language for it.
April 18, 2013, 12:33 AM
That's so great!:D
April 18, 2013, 12:40 AM
http://i47.photobucket.com/albums/f191/usmarine0352/ObamaBiden2ndCopy_zpsa0fcf018.jpg (http://s47.photobucket.com/user/usmarine0352/media/ObamaBiden2ndCopy_zpsa0fcf018.jpg.html)
April 18, 2013, 12:43 AM
Why does it make me so happy to see our "leaders" fail?
April 18, 2013, 12:55 AM
Why does it make me so happy to see our "leaders" fail?
Because they're leading us down the road to perdition.
April 18, 2013, 01:15 AM
They'll be back but so will we.
April 18, 2013, 01:46 AM
Who is the giant of a man behind uncle joe? SS?
April 18, 2013, 05:36 AM
Could be, he has a lapel pin, but it looks like the same one as the guy to the right of Biden.
April 18, 2013, 06:17 AM
Bunch of sore loser socialists! Obama was downright furious (or more accurately FUHRERous) looking in he had to acknowledge that we don't want his bull crap.
April 18, 2013, 07:09 AM
Joe is due for a beatin'.........
He needs a good stiff drink.
(those guys in the back are in indeed SS)
April 18, 2013, 07:19 AM
There's something missing from the photo....children.
April 18, 2013, 07:21 AM
I wonder how many evil guns were located within 50 yards of the podium? Yet nobody got shot.
April 18, 2013, 07:30 AM
Should be the next cover of NRA magazine..
Terry laughs riotously.
Terry, 230RN
April 18, 2013, 08:45 AM
Socialism loses some ground! Woot woot! :D
April 18, 2013, 12:22 PM
jdooner writes:
Biden was standing there with his arms crossed and a scowl on his face, he looked exactly like Jeff Dunham's Walter.
I think JD might have a new character in the works... Can a ventriloquist throw "Bidenese"? (of course, it's hard to throw your words when you're constantly tripping over them.)
April 18, 2013, 12:34 PM
now if only the SCOTUS would stay with the constitution....
April 18, 2013, 04:02 PM
I wonder how many evil guns were located within 50 yards of the podium? Yet nobody got shot.
Those guns were owned by government, and therefore they are not evil.
Only your guns and my guns and the guns in the hands of other law abiding citizens are evil. They go on assaulting people, while the governments guns are for personal defense.
See, the government got the well behaved and properly trained inanimate objects, we didn't.
April 18, 2013, 04:09 PM
(those guys in the back are in indeed SS)
Hitler's secret police??
April 18, 2013, 04:33 PM
Hitler's secret police??
Not sure if serious, but Secret Service?
April 18, 2013, 04:49 PM
This reminds me of something I read recently that was similar to Jeff Foxworthy's "You might be a Redneck if..." statements but was about America. Each of them went like this "if ______, you might live in a country founded by geniuses and now run by idiots." Here are the gun-related ones:
1. If you can get arrested for hunting or fishing without a license, but not for being in the country illegally,… you might live in a country founded by geniuses but run by idiots.
4. If the government wants to ban stable, law-abiding citizens from owning gun magazines with more than ten rounds, but gives 20 F-16 fighter jets to the crazy new leaders in Egypt, you might live in a country founded by geniuses but run by idiots.
12. If being stripped of the ability to defend yourself makes you more safe according to the government,… you might live in a country founded by geniuses but run by idiots.
April 18, 2013, 09:06 PM
Is that Michelle behind Obama in the first picture?
April 18, 2013, 09:09 PM
I just joined the NRA as a Life Member. This picture was well worth the $1000 check I sent. Seriously, I think I'll print that out and frame it over my reloading bench I'm building! :D
April 18, 2013, 09:11 PM
The facepalm looks like a bad Photoshop job. VP Biden had his arms crossed with a scowl on his face during the scolding President Obama was putting out.
April 18, 2013, 09:22 PM
Know what I see in that "shopped" photo?
How lovely Gabby Giffords looks, given the absolute hell she has suffered.
April 18, 2013, 09:23 PM
Classic. LOL
April 18, 2013, 09:45 PM
I'd really like to post how I feel about this picture, but would get banned from the forum! Being part of this forum is what I get up for in the morning, waiting to read what ya'll have posted, I want to keep up with the postings, so I'll mum it for now.
April 18, 2013, 09:51 PM
788HAm ...... I can relate to that statement!
Like You, I do enjoy coming to The High Road..... Have to think before I post sometimes, but they are in fact keeping us on The High Road.... :D
April 18, 2013, 10:10 PM
The facepalm looks like a bad Photoshop job. VP Biden had his arms crossed with a scowl on his face during the scolding President Obama was putting out.
I don't see it. But with thousands of pictures taken you are bound to get one of these.
April 18, 2013, 10:18 PM
If my kids acted like obummer I would spank their butts and send them to their room for a long time. It's time for him to grow up and accept he got beat at his own game.
April 18, 2013, 10:20 PM
There's something missing from the photo....children.
"Yay, we finally get to go home!" :D
"Bad photoshop"
It looks like a color/texture inconsistency on Bidens right arm where a new arm was grafted in, but you can see a matching wrinkle on his left as well (which is still in the "crossed" position). It's just his massive biceps pushing on the fabric, not a 'shop :D.
It's hilarious that Obama's image people couldn't keep everyone from doing this for the cameras. You know, the people that poke Biden in the back of the head with a stick to keep him from picking his nose or shooting spitwads at Boehner during the State of the Union.
April 18, 2013, 10:35 PM
They did have children there, they were to Obummers left. Of course he had to give them hugs after his speech for the camera.
April 18, 2013, 10:39 PM
Is this still The High Road.org?
Be careful that victories do not carry the seed of future defeats.
Ralph W. Sockman
April 18, 2013, 10:47 PM
This fight is not over and a little celebration is in order, but lets not forget the real story; nor let people forget. We have gun laws and back ground checks for firearm purchases; what we don't have is a functioning federal government that up-holds the law. Least we forget...
April 18, 2013, 10:48 PM
Beware of the threatened pres. Executive order!!!!!
April 18, 2013, 10:52 PM
This is not photoshopped. You can see Biden's pose in the first video on this page 30 seconds in.
April 18, 2013, 11:41 PM
They would have been dancing in the streets if the NRA would have loss!
April 19, 2013, 12:11 AM
This is not photoshopped. You can see Biden's pose in the first video on this page 30 seconds in.
http://www.weeklystandard.com/blogs/biden-wipes-away-tears-rose-garden-gun-remarks_717990.htmlNailed it!
April 19, 2013, 06:45 AM
I love how they just say "The NRA won". That's part of their problem right there. :D
For sure they would have been in glee if they had won the vote. Regardless of the chances of the same thing in the House and the real fact that such a law if passed would have no impact on lawbreakers. (yeah I know, that's not what it's really about)
April 19, 2013, 12:39 PM
The guys behind & beside foot in mouth joe are there to gag him if he opens his mouth.
April 19, 2013, 01:26 PM
This guy is delusional, and I will say it again, he thinks if he keeps repeating things that they will become true, Like those 10% evil people, beat our 90% Americans. I for one, never saw 90% get beat by 10%, any place for anything. But he keeps saying it, think how stupid it sounds. It's like saying 90% of America voted against gun control, and somehow the 10% still out voted them.
Where does this kind of logic occur? only in his mind.
He will insist and just talk over anyone who disagrees with him, and then Morgan and the rest of the Biased Media, will keep repeating it, those lousy 10% stopped us from being safe, until someone makes it evident that this is just a flat out lie.
If they had 90% we would be sitting here with slingshots, "maybe".Probably spit balls.
Bad Andy
April 19, 2013, 02:16 PM
April 20, 2013, 12:29 AM
I can't believe it! Biden REALLY DOES look like Jeff Dunnam's "Walter".
I almost cracked a rib!
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text:
National Alliance on Mental Illness
page printed from http://www2.nami.org/
(800) 950-NAMI; email@example.com
Baseball and Mental Illness: Something to Cheer About?
Photo: Keith Allison / Flickr
By Bob Carolla, NAMI Director of Media Relations
Who's on first?
As Mental Illness Awareness Week, Oct., 7-13, unfolds, baseball teams are slugging it out in play-off games that will ultimately lead to the World Series, Oct. 24-29.
In the Nashville Tennessean this week, E. Douglas Varney, commissioner of the Tennessean, Department of Mental Health and Substance Abuse Services, has published an article noting that 20 million Americans will watch the World Series on television.
Thousands more will spend an average of $500 to watch a game in person.
Yet according to figures compiled by NAMI in 2006, per capita spending by states on mental health services ranged from about $50 to only $250.
"Mental illness can happen to anyone, even the players on the field," Varney writes in the Tennessean. "In fact, San Francisco Giants' first baseman Aubrey Huff went on the 15-day disabled list earlier this season after he was diagnosed with anxiety disorder. Now he's leading his team in the playoffs."
That's something to cheer about.
And to think about during MIAW.
Related Articles
Athletes and Mental Illness: Major League Baseball Steps Up to the Plate
MIAW to Show Sucesses
MIAW Letter: Mental illness a non-partisan issue


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After a recent overhaul in its operations, CROSSWAEH now is on track in its goals to reduce criminal behavior and divert offenders from the state prison system, according to an official with the center.
Although the community-based correctional facility received a failing grade in 2010 in regards to its two-year post release program, the facility has since been working hard to fix it.
Jason Varney, vice president of correctional programs in Seneca County for Oriana House's CROSSWAEH facility, said after working closely with the University of Cincinnati for a year following the release of the study, the facility already has seen the impact of the changes.
Article Photos
CROSSWAEH, located at 3055 S. SR 100, includes facilities for men and women. The community-based correctional center is accredited by the American Correctional Association and also received 100 percent on its state audit this year.
CROSSWAEH clients Amanda Akers (from left), Tori Sheffield and Tabitha Bass sit inside of CROSSWAEH's female facility.
CROSSWAEH employee Tom Berry (right) assists a client at the male facility.
"The university provided us with the framework to make some changes or overhauls to programing structure," he said. "We changed some programs we provide but, for the most part, we added programming to the facility."
Varney said many of the programs had been in place, but they may not have been delivered properly, resulting in the poor recidivism score. Now, with the help of the University of Cincinnati, quality assurance of the programs has been upgraded.
CROSSWAEH, a facility accredited by the American Correctional Association, has a total of 94 clients in its female and male facilities. Clients come from nine regional counties and are offered cognitive behavioral programs, employment and educational services.
Many of the clients are felony offenders and are considered at a higher risk to re-offend.
The average stay of a client at CROSSWAEH is four to six months, Varney said, and programs with the main objective of reducing recidivism take up much of a client's typical day.
"One of the most important things, when an offender comes into CROSSWAEH, it's not just to do time, the expectation is that they will change their behavior," he said. "Our program has to be designed to effectively change their behavior for the six months they're here."
During a typical day at CROSSWAEH, clients wake up at 6 a.m., eat breakfast and begin daily appointments. Many see a caseworker, attend a meeting, perform community service or go to work, Varney said.
"There are a lot of commitments when it comes to having meetings or attending certain groups," he said.
After a break for lunch, clients return to daily appointments.
"In the evenings, there's not much programming," Varney said.
Clients will typically watch TV or work out before going to bed, he said.
"All of our clients are in bed by midnight," Varney said.
In order for a successful release, clients must complete three phases in which performance is directly tied to progress. Directly tying the two at the facility has been a change for the better, Varney said.
"We've seen a dramatic improvement in effort and desire to perform better to get out of program quicker," Varney said. "It really puts the responsibility in a program like CROSSWAEH on them. They know what they have to do and we know it kind of empowers them to do it quickly."
Rule violations also have diminished with the changes suggested by the University of Cincinnati.
"We used to process about 15-20 major rule violations every week. We now process maybe three or four rule violations every week. There's been a pretty dramatic improvement as far as rule compliance goes," he said.
Rewarding clients for good behavior through character coupons and weekly incentives also has been helpful in giving clients motivation to perform well.
All of the programs at CROSSWAEH aim to reduce recidivism of offenders, but that's not the only asset of community-based correctional centers. Varney said taxpayers also save quite a bit of money by sending an offender to a community-based correctional center instead of prison.
"The state prison capacity is 38,000 offenders, and currently, the state has about 50,000 in (the prisons)," he said.
To incarcerate one person, it costs taxpayers about $24,000 annually. To send someone to CROSSWAEH, it costs $8,000 in the same period.
"That's why it's beneficial from a fiscal standpoint," Varney said.
Jim Fruth, chair of the facility governing board at CROSSWAEH, said the facility is a great option not only fiscally, but because of its success in reducing recidivism.
"The rate of recidivism is a lot lower than when someone goes to prison," he said. "These people are receiving treatment and rehabilitation programming that they not would receive in an overcrowded prison system."


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|Rotary Firing in Ring-Shaped Protein Explains Unidirectionality
Hexameric motor proteins represent a complex class of molecular machines that variously push and pull on biological molecules using adenosine triphosphate (ATP) as chemical fuel. A specialized class of ring-shaped motor proteins, hexameric helicases, can unwind DNA strands and perform large-scale manipulations of single-stranded nucleic acids in processes such as DNA replication, DNA repair, and gene expression. To understand how certain hexameric helicases walk with directional polarity along single-stranded nucleic acids, Berkeley researchers used x-ray crystallography at the ALS to solve the structure of a hexameric helicase, the Rho transcription termination factor (from E. coli), bound to both ATP mimics and an RNA substrate. The results showed that Rho functions like a rotary engine: as the motor spins, it pulls RNA strands through its interior. Interestingly, the rotary firing order of the motor is biased so that the Rho protein can walk in only one direction along the RNA chain.
Crystallization trials were conducted around a matrix of RNA substrates and nucleotide mimetics and initially resulted in three forms that diffracted to between 4 and 6 Å. Utilizing the capabilities of ALS Beamlines 12.3.1 and 8.3.1, the researchers were able to carefully screen hundreds of crystals, overcome a series of unique crystal defects, and collect a dataset diffracting to 2.8 Å. The structure is composed of six unique Rho protein chains, six ADP·BeF3 molecules, and six bases of an rU12 RNA substrate. The Rho model, which is remarkably asymmetric, provides six independent snapshots of the motor mechanism, revealing how ATP turnover is converted to mechanical energy that propels the protein along its RNA substrate.
Inspection of the model reveals that Rho binds to RNA in a helical conformation, using a "spiral staircase" arrangement of RNA binding loops that project from five of the protein subunits to interact with the RNA's sugar–phosphate backbone. The sixth subunit does not significantly contact the RNA and lies midway between the top and bottom steps of the staircase. The positional relationships between the six Rho subunits generate four distinct classes of ATP binding sites that together represent a complete ATP turnover cycle. The organization of these sites around the ring indicates that ATP is consumed by a sequential mechanism akin to a rotary or radial engine. The cycle of ATP binding, hydrolysis, and release is carried out as each subunit passes through the six conformational states observed in the structure, creating a rotary wave motion within the RNA binding loops to power nucleic acid translocation in the proper direction.
Comparison of Rho's structure to that of the remarkably similar human papillomavirus E1 protein indicates that Rho and E1 translocate in opposing directions because the radial sequence by which its multiple intermediate ATPase states activate to catalyze ATP turnover is inverted. This mechanism is analogous to reversing the firing order of cylinders in an internal combustion engine to reverse the direction of crankshaft rotation.
By providing the first high-resolution views of hexameric helicase translocation intermediates, the structures of Rho and E1 have broad implications for understanding motor mechanisms in general and can be used to guide further research on this class of proteins. Recent research on Rho and other systems such as the ClpX protein translocase, the bacteriophage φ29 packaging motor, minichromosome maintenance (MCM) protein, and the bacteriophage φ12 P4 packaging motor suggest that variations of the Rho and E1 mechanisms likely exist. Given their fundamental role in all known forms of cellular life, it is likely that modulating, reengineering, or inhibiting the functions of hexameric helicases will lead to exciting applications in basic research, biotechnology, and nanotechnology.
Research conducted by J.M. Berger (Univ. of California, Berkeley, and Berkeley Lab) and N.D. Thomsen (Univ. of California, Berkeley).
Research funding: National Institutes of Health and the G. Harold and Leila Y. Mathers Foundation. Operation of the ALS is supported by the U.S. Department of Energy (DOE), Office of Basic Energy Sciences.
Publication about this research: N.D. Thomsen and J.M. Berger, "Running in reverse: The structural basis for translocation polarity in hexameric helicases," Cell 139, 523 (2009).


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The Fun and Art of Pumpkin Decorating
Pumpkin carving, painting, or decorating is one of those activities that can bring family members of all ages and descriptions together for a good time. It fun and a bit messy, which many kids enjoy. Parents can teach safety for use of sharp tools, while providing an opportunity for creativity to blossom. This activity, along with the family conversations that occur when you are playing together can make memories that last a lifetime.


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23 Totally Cool & Creepy Halloween Tattoos (Photos)Sunny Chanel
Halloween is a holiday predominantly celebrated by children. It's a day of dress up, school parades, trick or treating and lots and lots of candy. But even though many adults may be too old to ring bells for sweets, Halloween still has a soft spot in their hearts, and apparently also on their skin.
While tattoos celebrating Arbor Day or Ground Hog Day may be rare, getting a tattoo in honor of Halloween is pretty darn popular. Halloween's iconic images are a good match for tattoo art with bats, skulls, zombie and, of course, pumpkins.
Check out these 23 examples of tattooed homages to Halloween right here.
Which is your favorite?
Zombie Girl 1 of 23I hope this was a photo of the person's kid from Halloween that they just loved. Otherwise, well, it's just weird.
Photo Source: Merchant Circle
Trick or Treat 2 of 23Wow. Just wow. This is a great big chest piece that includes black cats, pumpkins, spider webs and - of course - candy corn.
Photo Source: Rate My Ink
Wide Eyed 3 of 23The sweetness of this little's boy eyes doesn't make this Halloween tattoo creepy, rather it makes it super cute.
Photo Source: Tattoo Inspiration
Bride of Frankenstein 4 of 23This is a very colorful rendition of the classic character - the Bride of Frankenstein. It's like every day is Halloween with her hanging around.
Photo Source: About.com
Pin-Up Witch 5 of 23There are all sorts of details added into this complex and detailed tattoo, from the naked witch to the fall hued squash.
Photo Source: Jason Stephan
Wicked Witch of the West 6 of 23This is a gorgeous tribute to the The Wizard of Oz and the very iconic Wicked Witch of the West.
Photo Source: Apropos of Nothing
Mummy Cat 7 of 23I can't figure out if this one is cute or creepy.
Photo Source: Tattoo Gathering
House on Fire 8 of 23This is a classic Halloween scene living on this person's arm. It's got pumpkins, a leafless tree, and a spooky mansion, a mansion that just happens to be on fire.
Photo Source: Rate My Ink
The Halloween Cupcake 9 of 23This isn't just an tattoo in honor of Halloween, it's in honor of the Halloween cupcake! Delicious.
Photo Source: Halloween Forum
A Two Parter 10 of 23This Halloween scene was too much for one limb, it continued onto a second. One thing, the shade and shadow work is fantastic.
Photo Source: Ink or Art
Bela Lugosi 11 of 23As Bauhaus informed us, Bela Lugosi may be dead but he is alive and well in ink form on this person's limb.
Photo Source: Sexy Tattoo Ideas
Black Cat & Co. 12 of 23The tattoo may look simple but it is chalk full of Halloween imagery, there are pumpkins, a graveyard, a witch, a demon and of course a black cat front and center.
Photo Source: God of Insects
Scary Halloween Kid 13 of 23This isn't just your average everyday trick or treater, this one is wielding a knife. Creepy.
Photo Source: Trent Edwards
A Colorful Halloween Scene 14 of 23Most Halloween tattoos are dark and moody, but this one is downright cheery with all its bright bold colors.
Photo Source: About.com
Herman Munster 15 of 23For a more campy ode to Halloween-ish characters, this person got a startled looking tattoo of Herman Munster on their body.
Photo Source: Sexy Tattoo Ideas
Trick or Treating with a Voodoo Doll 16 of 23You'd think with this much of a creepy costume the kid would have gotten more candy. But I do like how this zombie kid has his own zombie doll.
Photo Source: Gallery of Tattoos Now
Nightmare Before Christmas 17 of 23Someone really, really likes Tim Burton. This ain't no little tribute tattoo to the cult classic, this is a full on side piece.
Photo Source: About.com
The Halloween Tree 18 of 23A lovely way to pay tribute to Halloween, this woman inked a Halloween Tree on her arm. Perhaps she is a Ray Bradbury fan.
Photo Source: Adorn Body Art
Pumpkin Hand 19 of 23Now this is a commitment to loving Halloween. This person got a Jack O' Lantern tattooed on their hand.
Photo Source: Body Art Book
The Mask 20 of 23This is one of those tattoos that you just really want to know more about. Is it a portrait? Is it the person's child? And why do they look so bummed out. So, so many questions.
Photo Source: Guide Code Blog
Simpsons 21 of 23Every year The Simpsons do a Halloween episode and this person evidently is a huge fan.
Photo Source: Tattoo Mas Brow
Everyday is Trick or Treat Time 22 of 23To look at this trick or treating goblin every day, that is a commitment to Halloween.
Photo Source: Tattoo Gathering
Dreaming of Candy 23 of 23This skeleton wearing kid has one thought on their mind - candy!
Photo Source: Trent Edwards
Photo Source: Tattoo Now
Read more Sunny Chanel on Famecrawler right here.
Read more Sunny Chanel on Strollerderby right here.


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Hope I don't make you sea sick... I've got the baby sway going on!
(For a refresher, click on "View Videos" and then "My Uploaded Video" and then "Introducing Ivy LaRue")
Sweet name, sweet baby girl!ReplyDelete
Ask, and ye shall receive! I wanted more baby and I got more baby! Video! Squeeeeeeeeee! Those cheeks, no wonder you're smitten! So edible!ReplyDelete
I'm loving your videos! Love the name and the reason you picked it. (And if you haven't figured it out, I'm very partial to the name Ruth - love that LaRue plays into that too.) She is such a little bundle of love - so precious!ReplyDelete
SO, SO cute!! She's a doll, I love her name and you look fabulous!ReplyDelete
She is so beautiful! I love the way you chose her name.ReplyDelete
So sweet! I love the story of her name, and I agree, it is really classy.ReplyDelete
Oh I love it, what a beautiful reason for name! And I am LOVING the girly clothes.
Ahh, babies are so fun!
Im super impressed with the sweater you knitted her-I can barely do a scarf...lol!
Very sweet video! I simply adore her name and it is so fitting.ReplyDelete
She is adorable. I do love the name. And you look great...so rested and refreshed looking :)ReplyDelete
I love the video! You look great!ReplyDelete
I love her name, and I was wondering where LaRue came from so I am glad you explained. That is so special! I thought perhaps it was your tribute to the time you and Jeff spent in Paris. And love the Cubs link!
She is precious! When can I see her (and you) again?
I can totally get on board with a sweet girl named after a love for the Cubs....my Peyton got her name from a certain Indy quarterback. ;)ReplyDelete
So sweet, and so well done. Great job!ReplyDelete
Ivy is precious!!! My daughter heard you talking so she came over to the computer and sat on my lap. She kept pointing to Ivy and laughing. Keep the videos coming :)ReplyDelete
You are precious!ReplyDelete
She is so adorable!!!! Did you know that LaRue means "the Street" in french!ReplyDelete
Very nice....our daughter (Maddux) is our tribute to the Cubs - since Greg Maddux is the greatest pitcher ever, on and off the field.ReplyDelete


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REEDSVILLE - Leading a church takes teamwork.
The Rev. David Aungst has been serving Mountain View Chapel in Reedsville since February and will be officially installed during a service at 10:15 a.m. Sunday. But Aungst said leading a church is a group effort.
His wife, Elise, currently works as a substitute teacher in the Mifflin County School District, but also joins her husband in his ministry at the church.
Article Photos
The Aungst family, from left, Annalise, 7; Elise; David; Jake, 21 months; Ben 11; and Lydia, 9. The Rev. David Aungst will be installed as the new pastor of Mountain View Chapel during a service set for 10:15 a.m. Sunday at the church in Reedsville.
Photo submitted by DAVID AUNGST
"We operate as a team. We have a vision together," she said. "We're both called to serve Christ."
Together, the Aungsts aim to reach the community for the Lord. They said the church is part of their team.
"There's no big 'Is' or little 'yous,'" David said.
David is a native of Mifflin County and grew up in McVeytown, where he attended Strodes Mills Elementary and Middle School. After high school graduation, Aungst attended four years of seminary school at Pensacola Christian College, where he met Elise.
Aungst has been in ministry for 14 years, serving previous ministries as youth pastor in Mount Laurel, N.J., and senior pastor in Westfield, Pa.
Aungst said his call to the ministry came in 1995, when the pastor of the church he attended at the time did a series on decisions. He recalled a quote the pastor used, "The enemy to a best decision is a good decision." Aungst said the topic challenged him to choose the best for his life, which he found through serving the church.
In his new position at Mountain View Chapel, Aungst said his goal is to grow the church, both in spirit and attendance. He and Elise also hope to enhance already-existing ministries to reach more people.
"We're really excited about it," Elise said. "The heart of the people drove us here. This church has a vision for itself."
David said he had been considered to serve at numberous churches across the United States, but felt called to serve at the chapel in Reedsville.
"God ... He opened up the doors," he said. "It's just a blessing to be here."
Mountain View Chapel holds worship services at 10:15 a.m. weekly on Sundays. Aungst said services combine traditional and contemporary worship. Sunday School is held at 9:15 a.m. weekly on the same day.
The chapel also holds men's Bible studies at 9 a.m. weekly on Tuesdays and ladies' Bible studies at 9 a.m. weekly on Wednesdays.
For more information, call the chapel office at 667-3312 or visit www.mountainviewchapel.org.


text:
- Details
Electrical Safety Write-On Labels- Safety Check... By... Date
- Unmarked electrical equipment can result in serious injury; keep your crew informed of potential hazards with these convenient, easy-to-use, Electrical Safety Write-on Labels
- Choose from 2 materials:
- Vinyl cloth electrical safety labels have a special adhesive that sticks to most surfaces (flat, smooth, or curved), but can be easily removed or repositioned when needed, withstand temperatures up to 180°F
- Self-debossing foil electrical equipment inspection labels are made of all-weather aluminum foil for indoor/outdoor use, soft .0046 thick aluminum conforms to curved surfaces
- These adhesive tags are ideal for highly abrasive and high temperature environments as they withstand temperatures from -40°F to 266°F
- All safety labels measure 1-1/2"W x 5/8"H and can be written on with pen, pencil, or marker
- Electrical equipment testing labels are sold in packages of 350 (25 cards with 14 labels/card)
- This label reads "Safety Check, By, Date"
SpecificationsSelect options above to see specifications.
- Help
Need more details or have questions about this product?
We have experts on staff to answer all your questions Monday-Friday, 7:30am to 7:00pm EST


text:
Winston-Salem, NC (Sports Network) - Mason Plumlee scored a career-best 32 points, grabbed nine rebounds and steered fifth-ranked Duke away from an upset bid and past Wake Forest, 75-70, at Lawrence Joel Veterans Memorial Coliseum.
Wake Forest focused on stopping Duke's dynamic perimeter game, leaving Plumlee to work one-on-one, many times against smaller defenders. He took advantage with a 12-of-15 effort from the floor to go along with a pair of blocks. Seth Curry added 21 points and Quinn Cook donated 12 for the Blue Devils (18-2, 5-2 ACC), who made just 2-of-14 threes one game after connecting on 11 against Maryland.
Devin Thomas led Wake Forest (10-10, 3-5) with 15 points and six rebounds, while C.J. Harris and Travis McKie each added 14 points. The Demon Deacons nearly pulled off the upset just over a week after downing North Carolina State in the same building.
The Sports Network


text:
Job description
An internship or co-op with CIGNA will provide you with invaluable insight into the
professional world and your future career path. Engage in meaningful project work
that directly impacts our business, interact with senior leaders in an informal setting,
develop strong industry knowledge and functional skills, and network with employees
and interns across various locations.
Undergraduate Opportunities May Include:
Actuarial Science
Clinical: Behavioral, Nursing and Pharmacy
Human Resources
Information Technology
Graduate Opportunities Include:
General Management
Preferred Qualifications
• Bachelors or Graduate degree in progress
• Strong leadership skills and involvement in campus organizations
• Strong interpersonal skills and customer focus
• Ability to learn on the fly
• Excellent oral and written communication skills
• Strong problem solving ability
To Learn More Please visit http://careers.cigna.com and visit the University Relations Home Page Co-op Opportunities are also available
in select locations and functional areas for students participating in a recognized undergraduate cooperative study program.
Opportunities for co-op positions will be posted through your school.
Highlights of the CIGNA Summer Experience include:
Welcome Reception – Network with fellow interns, managers, and senior leaders
Executive Speaker Series – Hear from our executive leadership team and other senior business leaders in an informal
setting – learn about their business area, their career path, ask questions, and get valuable career advice
Breakfast with the CEO – Learn about the direction of the organization, and ask questions of our President and CEO over an informal breakfast
Community Service – Spend a day with fellow interns, giving back to the community in which you're working
Life After College Presentation – Learn tips for transitioning from college to the "real world"
Generational Differences – Learn to successfully work with and understand the four generations currently in the workforce
Poster Session – Create a visual presentation to share your accomplishments with a large audience of CIGNA employees, managers, and senior leaders
University Relations Scholarship Program – Performance based scholarships awarded to the summers' top interns
Hartford, CT
Philadelphia, PA
Opportunities may also be available in select locations nationwide
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Hostal Catedral
Set around a traditional central courtyard, this 19th-century house is situated in the La Judería district of Seville. Rooms have access to shared bathrooms and free Wi-Fi is available.
The guest house is decorated with authentic Andalusian tiles and features bright rooms.
Pension Catedral operates a 24-hour reception, allowing guests flexibility during their stay. The tour desk can offer information on Seville's many attractions, including the cathedral, Alcázar and Torre del Oro.
-All rooms are privated and have shared bath.
-Most rooms with exterior windows
-Most rooms with wash basin (sink) in room.
-Some rooms with air conditioning and heating 3€ per day supplement
-Blankets and towels are included
-Parking is available upon request. Costs 18€ per day
Parking is available upon request. Costs 18€ per day
Availability & RatesTo view availability and prices please select dates and click 'Update Dates'
B&B Facilities & Policies
- Air Conditioning
- Book Exchange
- Card Phones
- Credit Cards Accepted
- Free City Maps
- Free WiFi
- Hair Dryers
- Late check-out
- Luggage Storage
- Parking
- Reception (limited hours)
- Towels Included
Cancellation PolicyThis property has a 3 day cancellation policy. Failure to cancel within this time will result in a cancellation charge equal to the first night of your stay.
Must-Read Guesthouse InfoThis property has specific Terms & Conditions.
Reception is opened from 8am to 22.45
Check in time from 14:00 to 22.45
The hotel reserves the right to pre-authorise credit cards prior to arrival.
Note : we will charge the first night after the booking is made. In case you cancel on time, we will refund you the amount charge by us
DirectionsShow MapHide Map
Pension Catedral are located in the district of Santa Cruz, between the Puerta Carmona and Puerta la Carne just a few min from Pilate's House. The main monuments like the Cathedral and the Alcazar are less than 10 min. walk.
The Carbonaria is a very famous local flamenco in Seville night where you can see shows for free and is very close to the property. the property. Guests can walk to the Santa Justa train station or bus from Prado de San Sebastian.
Approx. walking distances: Pilatos House – 5 min. Catedral y Alcázar – 8 min. Encarnación square – 10 min. Shopping Center – 12 min. Guadalquivir river- 18 min. Plaza Mayor – 12 min. Alfalfa square – 10 min. Betis street – 18 min. Bus zone – 5/15 min.
Located Near
Hostal Catedral is situated in the Santa Cruz neighborhood, between the Puerta Carmona and Puerta de la Carne, just a few minutes away from the Casa de Pilatos. The major monuments such as the Cathedral and Alcazar are less than 10 minutes away. La Carboneria, a famous nightlife spot to enjoy free flamenco performances, is just a few blocks from the hostal. Internetia, one of Seville's largest internet cafes, is also just 2 minutes from the hostal. Guests are within walking distance of the Santa Justa train station, as well as the Prado regional bus station.


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on March 21, 2013 - 12:01 AM
, updated March 21, 2013 at 12:19 AM
The dust is still settling from Tuesday's management earthquake at First Niagara Bank, in which its chief executive officer, John Koelmel, was ousted from his position in a rift with the bank's board of directors. It was an unsettling development for several reasons, the two most prominent of which are First Niagara's important role in the economy of Western New York and Koelmel's significant influence as a passionately engaged civic leader.
Business is business, of course, and early reports indicate dissatisfaction by investors and the board with the bank's performance. First Niagara's stock price has suffered with its aggressive expansion program, particularly its acquisition last year of 1 million accounts from HSBC Bank.
It's the kind of problem that can cause friction, and Koelmel wouldn't be the first CEO forced out for that kind of reason. But those events are usually handled with greater aplomb than the sudden and unexpected expulsion of a chief executive. The soon-to-be-terminated executive is typically allowed to announce that he is moving on and the board thanks him for his service. That didn't happen and the tactlessness of the dismissal raises questions of whether other factors are at work here.
Whatever those factors may be, it is important for First Niagara to find the leadership it needs to continue its role in the local economy. With Koelmel at the helm, First Niagara became a homegrown powerhouse, with 6,000 employees, and a fixture in Buffalo, where it moved its headquarters in 2009.
If Koelmel's leadership was not what First Niagara needed going forward, Western New York has certainly reaped the benefits of his rising stature in the region's civic life. Most prominently, he serves as chairman of the New York Power Authority, which is a player statewide but of special importance to Western New York.
He is also chairman of the Kaleida Health board, a position he has held for nearly three years. That, too, is a critical post, given the rapid development of the Buffalo Niagara Medical Campus, which includes several high-profile outposts of Kaleida, and the corporation's prominent role in Great Lakes Health System, a combination of Kaleida, Erie County Medical Center, the University at Buffalo and the Center for Hospice & Palliative Care.
Koelmel also serves on the Western New York Regional Economic Development Council, where he is well-placed. In 2010, he worked to revive the Empire State Games, authorizing a $500,000 donation from the bank in hopes of drawing other donors.
Successful communities need people like Koelmel advocating for them. They are part of the necessary leadership package, and while Buffalo is blessed with many such private-sector leaders, including Robert Wilmers of M&T Bank, developer Howard Zemsky and Robert Gioia, president of the John R. Oishei Foundation, those men have been community leaders for many years. Koelmel is a relative newcomer to their ranks, and he is a powerhouse. We hope he stays here.
Western New Yorkers need to hope that First Niagara Bank finds the leadership it needs to remain a source of pride here and that Koelmel isn't tempted away to another region. This one is happy to have him.


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Top Definition
She was cute, but she could clear the dance floor with one kardashian.
додав Hank TADD 11 Квітня 2009
Trending Now
Can you define these popular missing words?
(adj.) A "Kardashian" is a unit of measurement representing 72 days of marriage, as ratified by the Anonymous Measurement Council.
Also refers to a female who is vapid, shallow, and burdened with a large posterior.
Also refers to a female who is vapid, shallow, and burdened with a large posterior.
I've been married for 10 Kardashians... I feel so old!
додав Artemis Crouch 03 Листопада 2011
any woman who is fake, cheap and easy.
That tacky bitch has fucked half the football team. She's so kardashian.
додав They rapin' errrybody out here 10 Серпня 2010
A Kardashian is someone who is completely useless, and or, worthless.
Dude, that guy is such a loser, what a Kardashian!
додав Truth_Teller23 01 Лютого 2011
A beautiful nobody........famous only thanks to physical attributes and free exposure via the internet.
Seems like everyday the world is in love with the latest Kardashian ....what have we become ?
додав 2m8rh8r 23 Лютого 2010
Human princess with a large posterior who often enjoys the company of black human males and utilizes modern technology to exhibit such sexual encounters. Often done to the dismay of a deceased father.
Kim Kardashian is a piece of hawt, steamy, female.
додав willy limp lump 14 Листопада 2007
(adj.) what results from pimping oneself out in the context of lowest-common-denominator pop-culture, selling yourself to all comers, living a life completely constructed and organized for this purpose
"The catering was free, 'cuz they promised to mention her in US magazine..... Man, how Kardashian is that?"
додав dégoûté 12 Серпня 2011


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92,508 words (≈ about 6 hours)
A violent, epic, action-packed urban quest full of very eccentric, often hilarious, extremely dangerous characters who also happen to be animals — the wildlife of New York City, to be exact.
A revised version of this book (also freely available on Feedbooks, as Beasts of New York 2.0) won a 2011 ForeWord Book Of The Year Award and has been published by the prestigious literary press The Porcupine's Quill.
To learn more, visit the book's web site: www.beastsofnewyork.com… (more)
Language: English
Written in: 2007
Published: 2009-01-03
Word count: 92,508 words (≈ about 6 hours)
License: Attribution Non-Commercial No Derivatives (cc by-nc-nd)
Tags: urban fantasy, new york city, Central Park, fantasy, animals, quest
Jon Evans is a novelist, journalist, adventure traveller, and software engineer. His novels have won Arthur Ellis and ForeWord Book Of The Year awards, translation into half a dozen languages, and praise from The Economist, The Times, and The Washington Post.
Jon's journalism has appeared in Wired, Reader's Digest, The Guardian, The Globe and Mail, and The Times Of India, and he writes a weekly column for TechCrunch.
Jon has released all of his published novels - Dark Places, Blood Price, Invisible Armies, The Night of Knives, Beasts of New York, and Swarm - under a Creative Commons license, and all can be freely downloaded from Feedbooks.


text:
iHerb Customer Reviews
Savings of: $0.44 (8% off)
hylands ferrum phos
Posted by Reviewer2445419 on Oct 30, 2010
works so well
Good product
Posted by keys2realhealth on Feb 03, 2012
This product is good for inflammation in general as well as for relieving headaches and fever.
Haven't tried this or this brand but guess it works just as well as other brands.
Posted by Reviewer2778088 on Nov 16, 2010
Good price :-)
short term
Posted by Reviewer3278845 on Sep 05, 2009
this product helps somewhat, it has 12 tabs thats dissolve but they go quick as the effects are short term, for once in awhile they are worth it but if needed long term i would say no as too many would be needed.
Posted by Reviewer1022036 on Jan 27, 2008
I bought this for homeotheraphy treatment for my dog. it appears to be working.
Items 1-5 of 5 total


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quote: Northern California is the northern portion of the U.S. state of California. The San Francisco Bay Area (which includes the cities of San Francisco, Oakland, and the largest city of the region, San Jose), and Sacramento (the state capital) as well as its metropolitan area are the main population centers . It also contains redwood forests, along with the Sierra Nevada including Yosemite Valley and Lake Tahoe, Mount Shasta (the second-highest peak in the Cascade Range after Mount Rainier in Washington), and the northern half of the Central Valley, one of the world's most productive agricultural regions.
quote: Generally the accepted standard is LA and surrounding areas are "Southern," while SF and surrounding areas are "Northern." What's in between, and north of SF, is irrelevant. :)


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Gun season for deer is under way and that means it's a good time to recall basic tips for staying safe and having an enjoyable time hunting.
First, know where you are, and where members of your hunting party are.
Don't hunt where your shots could reach homes or highways. You're not hunting property, people or automobiles, after all. That means you must know the range of your weapon and your ammunition.
Be careful of your own health, and know your limits. The hike alone is tiring, let alone a hike with a gun, or later, hopefully, with an animal to retrieve.
Do not head off intoxicated with a firearm. That's a recipe for disaster.
Annually, there are stories of hunters who have heart attacks in the woods. It's best not to hunt alone, and it's always a good idea to have a cell phone or walkie-talkie handy, just in case of an emergency. Wear bright clothing to be visible.
Be sure your weapon is unloaded before transporting it, and don't leave your gun visible in an unoccupied vehicle. Thieves are ready to pick up a new gun the easy way.
If you're bringing a new member of the hunting family into the woods, teach them the proper respect for firearms and fellow hunters.
Be sure your target is in front of you and that your field of fire is clear of people. Be sure you've got a clear bead on the target and you're not just shooting at motion. That's how hunters shoot one another.
Unload your gun before climbing a fence or climbing into a tree stand. If you think you're falling asleep in the tree stand, secure your weapon. Wear soft earplugs to protect hearing. Above all, respect yourself and your fellow hunters. That's how a tradition that goes back to the beginning of life in the area can be preserved for years, and generations, to come.


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Listen up Texans! AMERICAN IDIOT has a bunch of dates in the Lone Star State this season, three of which go on sale this Friday. The national tour will play the Majestic Theatre in San Antonio for 3 shows only, the Plaza Theatre in El Paso for one show only and the Wagner Noel PAC in Midland-Odessa for one show only. Your exclusive pre-sale info is below. Get your ticket before they go on sale to the general public!
Login now to Idiot Nation: HERE


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The New Chameleon CXS version 2 component separates are a truly innovative new speaker system. Their easy to change tweeter mounting allows you to choose the best mounting method for your vehicle. Installation versatility was only one of our goals when engineering the CXS separates. We set out to attain a level of fidelity that is unrivaled at any price. The result is a speaker system that provides superior resolution of details through the critical midrange and high frequencies with incredible dynamics and sonic realism. Their superior performance will be immediately apparent with your first listen of the CXS components.
Description Power Sens Freq Response Depth
CXS64 V.2 6.5" 4 OHM Component 25 -250w 90db 65Hz - 25kHz 3.0"


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A Starving Actor's Books
At A Starving Actor's Books we offer an eclectic blend of out-of-print, rare and gently used editions. Our titles cover the worlds of art, cooking, architecture and design, among others, and our inventory includes signed first edition volumes. We have something for every reader and collector, from beginners to discerning experts.


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Ranked among the nation's most integrated healthcare systems, Community Health Network is Central Indiana's leader in providing convenient access to exceptional healthcare services, where and when patients need them—in hospitals, health pavilions, workplaces, schools and homes.
We're ready when you are. Register for a free weight loss seminar.
Call 800-777-7775 today!
If you are at least 100 pounds or more overweight, losing weight is not just a good idea - it is a medical necessity. Every week you wait and every pound you gain adds an increasing risk to your health and life. Severe obesity is difficult to treat with traditional diet and exercise. It often requires medical help to find the best, healthiest way to take off a significant amount of weight. When you're ready for a change, Dr. Steven M. Clark and the team of bariatric specialists at Community Bariatric Services - South can help you understand your bariatric surgery options.
If you struggle with obesity, you should seek advice from the experienced bariatric specialists at Community Bariatric Services - South. Attend a free information seminar to learn more about obesity and surgical weight loss options. Bariatric team members will review surgery options, nutrition plans and lifestyle changes, and answer your questions. Dates and registration >>
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Our program, based at Community Hospital South in Indianapolis, Indiana, pulls together an impressive group of professionals to help you make informed choices. Through our team approach, we can help you shed and keep off meaningful amounts of weight. Our top surgeons and nurses work closely in collaboration with an expert dietitian and a prominent psychologist practiced in weight loss issues to ensure excellent service and outcomes.
If bariatric surgery is the right path for you, we will give you extensive support before and after surgery so you can be successful. If bariatric surgery is not a good option for you, we can direct you to the resources and experts you need for other medical weight loss solutions.
The South bariatric services team includes a skilled bariatric/general surgeon, a psychologist trained in eating and weight-related issues, and a full program support team that includes a physician assistant and dietitian. Together, our team can help you achieve your lifestyle and weight loss goals and help you get back to a healthier and happier you! Learn more about the South bariatrics team below:
Community Bariatric Services - South
Community Hospital South
1550 East County Line Rd., Suite 315
Indianapolis, IN 46227
Phone: 317-887-7771
Toll-Free: 855-277-7771
Fax: 317-497-2510
See more of our awards
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Remember that little nursery rhyme about the Farmer in the Dell?
It was a cutsey sing-song about how the farmer takes a wife, who takes a cow, who takes, um, a pig or something and eventually, the whimsical childhood ditty winds all the way down reveal a mouse who takes the cheese which, ultimately, stands alone.
As a child, I guess I never really understood the fundamental point of the cheese standing alone ... that is, until a few weeks ago.
Because that's when it became patently clear to this more-than-middle-aged woman precisely how and why the cheese does indeed stand alone. And the reason is: the mouse is too smart to take the bait.
This I discovered as a happy clan of mice danced around the bits of cheese, gobs of peanut butter and um, I think, some slices of $85-an-ounce goose liver pate which my husband had so delicately placed inside the mousetraps he'd dotted our camper with sometime back in November - in a vain attempt to keep mice (et al) from taking up residence within our formerly happy place.
Happy it was not on that cold spring morning, my friends.
Yes, 'twas the first part of April when all through the camper Mice were playing and laughing, shoot, I think I even saw one scamper.
In fact, the rascally rodents were literally frolicking inside our now decidedly-grumpy place as we flung open the door of the camper to open it for the 2013 season and found the vermin which methodically, effectively and with full malice, erased the collective grin from three Kimerer camper-er countenances.
Major, major hmpf.
True, we've been down this tiny road before it was not the first time I'd found raisin-esque deposits inside my skillet, pillowcase, couch cushions, sink and well, you get the idea.
Let me tell you something, folks: it is one thing to open your camper on the first day of the season and see evidence of small, furry, long-tailed intruders having crashed there during the cold of winter and fled at the sound of incoming humans.
But, it is something else entirely to witness them gleefully playing Parcheesi at your kitchen table.
"Hey, could you shut that? It's still a little chilly out there," said non-Mickey Mouse.
Chimed in non-Minnie with a tiny smile, "Yes, if you wouldn't mind. And, we're running low on snacks; could you be a dear and just set some nacho crumbs there on counter?"
Folks, there's just not a hmpf big enough for a germ-o-phobe such as yours truly to express my disgust at the vision which would become my nightmare for a solid week.
And just like that, I posted this ad e-Bay:
"Selling two-year-old camper. Holds great memories and sleeps up to 10. Like new; except for nooks, crannies, corners and other various spots chewed through, soiled upon and mocked by various members of local rodent community. All reasonable offers considered. And some non-reasonable ones. Heck, buy me some Dunkin' Donuts gift cards and we'll call it even."
Kerry deleted it before I even got one bing.
Needless to say, my hands are now sans that layer of epidermis which is generally considered helpful.
Yep, I bought every last bottle of cleaning solution on the shelf at the nearest market and proceeded to douse the place in germ-eradicating effort with a concoction of anti-viral, anti-bacterial, anti-mouse poo cleansers so potent in that, of course, it made me sick. A small mushroom cloud still faintly hangs over the slide-out.
Hmpf / sigh.
Old buddy Fred Callahan of Kinsman didn't realize I am still in mourning for my dead layer of skin and olfactory senses. When he heard of our unsightly stow-aways, he glibly chirped, "You don't really want to hang out in a place that even mice wouldn't live in, now do you? It's a sign of a desirable destination; consider it a compliment."
Fred is dead to me.
Unlike the pests who continue to try to edge us out of our very own newly-disinfected and soon-to-become-happy-again place.
Forget it, non-Mickey and Minnie - unless you plan to sanitize yourselves and start forking over rent, you are formally on eviction notice. This camper's not big enough for the - I don't know - 48 of us?
Shudder / hmpf.
Better watch those cute little whiskers, because it is on
Kimerer is a camper enthusiast - but only within reason. Contact her with ways to shoo away unwanted houseguests at email@example.com.


text: quote: "The Federal Reserve was clearly the steward of lending standards in this country. They chose not to act. The Federal Reserve Bank of New York certainly could have reined in what was being done in some of the large money-center banks in New York. I mean, on and on and on, regulator after regulator, they either chose not to act or turned a blind eye to what was actually going on. So it's less about a particular individual than a systematic sense of deregulation and inaction by those who were in power to take action."


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Aviation Management Program
Westminster's Aviation Management Program
Why Study Aviation Management at Westminster?
Our aviation management curriculum is heavily integrated with business classes in law, finance, management, and marketing. It also features specialized courses in aviation law, operations, and management. Students graduating with the aviation management major are prepared for many different types of positions in the aerospace industry.
The aviation management option is well-suited for the student who wants to combine his or her interest in aviation with a solid background and experience in business studies. Some of our aviation management students decide to also earn pilot ratings so they can fly; others decide to focus completely on the business aspects of aviation.
The Westminster Difference:


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Except where noted, MP3's are available at 320 kbps -- plays on iPod® and all MP3 players
Item Description
Simply titled, Michael Black, this album is Michael's first solo CD and was produced by John Doyle. This CD features a variety of songs from shanties, to a song in Irish, anti-war songs and a number of chorus songs. Musicians on the album include John Doyle, Liz Carroll, Liz Knowles, Seamus Egan, John Williams, Chico Huff, Dirk Powell and backing vocals include John Doyle, Mary Black, Frances Black, Martin Black, Shay Black, Eoghan Scott, Danny and Roisin O'Reilly.
Customers who purchased Michael Black also purchased:


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|Structural Basis for Activation of Cholera Toxin
Cholera is a serious disease that claims thousands of victims each year in third-world, war-torn, and disaster-stricken nations. The culprit is the bacterium Vibrio cholerae, which can be ingested through contaminated food or water and colonizes the mucous membrane of the human small intestine. There, it secretes cholera toxin (CT), a protein whose A1 subunit (CTA1) triggers a series of events culminating in the massive efflux of electrolytes and water into the intestinal cavity, causing the watery diarrhea characteristic of cholera that can lead to severe dehydration and death. Crystal structures of the CTA1 subunit in complex with its activator molecule (ARF6) reveal that binding of the ARF6 "switch" elicits dramatic changes in CTA1 loop regions, exposing the toxin's active site. The extensive CTA1–ARF6 interface mimics recognition of ARF6's normal cellular protein partners, which suggests that the toxin has evolved to exploit the molecular switch's promiscuous binding properties.
After a molecule of cholera toxin binds to an epithelial (lining) cell of the human small intestine, CTA1 can enter the cell via several organellar trafficking systems. Once inside, CTA1 interacts with a protein known as adenosine diphosphate (ADP)–ribosylation factor 6 (ARF6), which enhances the activity of the toxin. Almost thirty years ago, ARFs were originally discovered and defined as activators of CTA1. They have since been revealed to also play essential roles in the trafficking of vesicles within cells during normal physiological conditions. While this multispecific binding strategy gives ARF proteins broad flexibility, it also leaves them vulnerable to interaction with unintended partners.
Several crystal structures of both cholera toxin and ARFs have been published; however, it is not clear how these two proteins interact, and more interestingly, how ARF interaction enhances the cholera toxin's activity. To investigate, researchers from the Howard Hughes Medical Institute, the University of Washington, and the University of Colorado Health Sciences Center turned to ALS Beamline 8.2.2 to determine the 1.8-Å-resolution crystal structure of a CTA1 variant bound to a complex of human ARF6 and guanosine triphosphate (GTP), a mediator of the interaction.
CTA1 makes two large conformational changes when interacting with ARF6-GTP, both in loop regions indicated by previous structures to be flexible. The CTA1 activation loop changes from a structured loop to an amphipathic (polar) helix to make direct contacts to ARF6-GTP. Most strikingly, the active-site loop, which normally occludes the active site and prevents the binding of substrate (target) molecules, swings out of the active site when CTA1 is bound to ARF6-GTP, exposing areas along the active-site cleft implicated in substrate binding. The active-site loop does not directly contact ARF6-GTP; instead, the presence of the activator is communicated from a distance by several contacts with the activation loop in its altered conformation. The structure thus implies that ARF6 acts as an allosteric activator of CTA1; that is, it forces the active site open by binding at a site other than the enzymatically active one. This conclusion was confirmed by soaking CTA1:ARF6-GTP crystals with nicotinamide adenine dinucleotide (NAD+), the substance that binds to the active site. The resultant structure was solved at Beamline 19-ID of the Advanced Photon Source and provides the first glimpse of substrate binding by CTA1.
Further, the open active-site loop conformation creates a knob on the surface of CTA1 near a motif (the "ADP-ribosylating turn-turn" motif) known in functionally related toxins to be involved in binding to the human protein targeted by the toxin. This suggests that, in addition to activation, interaction with ARF6 also generates a surface on CTA1 enabling the toxin to recognize its human protein target. Together, these results elegantly solve the long-standing question of how the human ARF6 protein activates CTA1, while the substrate-bound structure also provides a starting point for structure-based drug design specifically aimed at cholera.
Research conducted by C.J. O'Neal (University of Washington), M.G. Jobling and R.K. Holmes (University of Colorado Health Sciences Center), and W.G.J. Hol (Howard Hughes Medical Institute and University of Washington).
Research funding: National Institutes of Health and Howard Hughes Medical Institute. Operation of the ALS and the APS is supported by the U.S. Department of Energy, Office of Basic Energy Sciences.
Publication about this research: C.J. O'Neal, M.G. Jobling, R.K. Holmes, and W.G.J. Hol, "Structural basis for the activation of cholera toxin by human ARF6-GTP," Science 309, 1093 (2005).