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I have been using word press without any issues for over a year. Last week I noticed upon uploading an image that only the Full size was selectable. The pixel size is also not indicated anymore . It's labeled as (0 x 0). Thumbnail, medium and large have no size underneath the check box and are not selectable.
What can cause this from happening? Is there a fix for this.
thanks for your time


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- Report: #258078
Report - Rebuttal - Arbitrate
Complaint Review: GIECO INSURANCE
GIECO INSURANCEgieco.com Nationwide U.S.A.
GIECO INSURANCE Gieco had me paying a monthly premium for full coverage and now does not want to pay for my vehicles total loss Macon Georgia
*Consumer Comment: Go to your local public library and ask for the "NADA Orange Book"....
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I have visited Mercedes dealership who had my exact same model and similar milage and they were selling that car for almost $15,000 dollars, and among the other quotes we have gotten and forwarded to Gieco and not they don't answer the phone or return calls. In the mean time I am paying $505.00 a month for a car I don't have and truly can not afford to continue to pay for since I had to replace the car with another vehicle.
I have never missed a payment on my insurance nor have I had an accident. speaking of accidents, the babysitter took my car without my permission while I was out of town and scratched someones car. The babysitter did a depostion stating that they took the car without permission and stated that they have never driven my car before (also offered to pay for the accident)and Gieco put it on my driving record and clue report as an accident that I personally was at fault at.
CHICAGO, Illinois
This report was posted on Ripoff Report on 07/01/2007 01:38 PM and is a permanent record located here: http://www.ripoffreport.com/r/GIECO-INSURANCE/nationwide/GIECO-INSURANCE-Gieco-had-me-paying-a-monthly-premium-for-full-coverage-and-now-does-not-w-258078. The posting time indicated is Arizona local time. Arizona does not observe daylight savings so the post time may be Mountain or Pacific depending on the time of year.
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#1 Consumer Comment
Go to your local public library and ask for the "NADA Orange Book"....
AUTHOR: Thomas - (U.S.A.)
SUBMITTED: Sunday, July 01, 2007POSTED: Sunday, July 01, 2007
Note that in some states an insurance company must only pay you the 'trade-in' or 'loan value' for a total-loss vehicle, while in other states you are entitled to receive the 'retail price'. Contact your MVD.
Also note that your friendly Mercedes dealer may, if they wish, ask for many thousands ABOVE the NADA Retail Price. And usually they WILL wish to ask for many thousands ABOVE the NADA Retail Price. This gives the dealer room to negoiate with a buyer and maybe that buyer will 'steal' that used car- by paying full NADA Retail! Also the buyer can get killed on his trade-in allowance if he does not realize the used car asking price is padded w/r/t NADA.
You are (now) discussing an (almost) 8 year-old car which likely is worth less than 20% of the value of a new Mercedes C230 ($30,000 list?).
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Per the U.S. DoD, * Indicates a "small business" classification and ** indicates a "small disadvantaged business". NOTE: the U.S. DoD only publicly reports contracts valued at $6.5 million USD or greater. The contract language is EXACTLY as it appears on the official U.S. DoD website (http://www.defense.gov/contracts/) unless otherwise noted.
Principle Contractor: Alliant Techsystems, Incorporated
Department: Army
Contract Details: Alliant Techsystems Inc., Plymouth, Minn., was awarded on Jan. 29, 2009, a $29,729,805 firm fixed price modification for the manufacture and delivery of approximately 3,240 M829 A3 120mm Armor Piercing, Fin Stabilized, Discarding Sabot Tracer Cartridges. Work will be performed at 21 stateside locations with an estimated completion date of Apr. 11, 2011. One bid was solicited and one bid received. U.S. Army Joint Munitions and Lethality LCMC, Picatinny, N.J., is the contracting activity (W15QKN-07-C-0085).
Total Contract Value: $29,729,805
View all contracts involving "Alliant Techsystems, Incorporated"


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- Report: #471932
Report - Rebuttal - Arbitrate
Complaint Review: BankCard USA Merchant Services
BankCard USA Merchant Services5701 Lindero Canyon Road, Building 3 Westlake Village, California U.S.A.
BankCard USA Merchant Services Will not release money and will not return emails or phone calls. Westlake Village California
*UPDATE Employee: BankCard USA Rebuttal-please read
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There was no communication from this company explaining any problem or requesting any additional information. We have made multiple phone calls to this company and either land in a mailbox that is full or receive no response to our message. After several phone calls I was finally able to obtain details on what information needed to be sent and who to send them to. Before we could even get that email sent, and email from Heather Campbell (firstname.lastname@example.com) arrived:
"I'm writing in regards to the merchant account and the funds on hold. The merchant account was approved for 100% keyed activity with an average ticket of forty eight dollars and monthly volume of three hundred dollars; the agreement also states all transactions will be for web hosting with a forty eight hour maximum fulfillment.
There are several concerns that Risk Management has that we feel need to be addressed;
You have processed sales that exceed the approved average ticket
You are more than double the approved monthly volume
Unfortunately, your personal credit is derogatory
Seeing the above listed concerns, Risk Management is no longer comfortable processing your company's credit cards. I will need you to issue a return to the two transactions that Risk Management is holding and obtain another form of payment. Once the returns are issued, the merchant account will be closed.
Please issue returns to the following credit cards"
We have left numerous messages for Heather and others at the company. We have sent documentation to verify the orders made on July 15th. There is still no response from anyone at the company. They have essentially destroyed our business and our livelihood and they appear to care less about us as customers.
If you are considering this company for merchant services, RUN AS FAST AS YOU CAN! They are unscrupulous and some of the worst con-artists I have ever dealt with in 28 years as a small business owner. I have already filed a complaint with the Santa Barbara Better Business Bureau where over 110 complaints have been filed against this company in the past 36 months.
Kansas City, Missouri
This report was posted on Ripoff Report on 07/21/2009 03:45 PM and is a permanent record located here: http://www.ripoffreport.com/r/BankCard-USA-Merchant-Services/Westlake-Village-California-91362/BankCard-USA-Merchant-Services-Will-not-release-money-and-will-not-return-emails-or-phone-471932. The posting time indicated is Arizona local time. Arizona does not observe daylight savings so the post time may be Mountain or Pacific depending on the time of year.
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#1 UPDATE Employee
BankCard USA Rebuttal-please read
AUTHOR: Sharon - (U.S.A.)
SUBMITTED: Wednesday, October 28, 2009POSTED: Wednesday, October 28, 2009
We are responding to a complaint filed by the above referenced merchant. In reviewing the merchant's complaint and processing history, we have concluded to the following.
1. The merchant applied with BankCard USA to ebnable their business to start accepting credit cards from their customers. They were approved for specific terms and conditions. These are their approved parameters per their signed merchant agreement with BankCard USA.
2. The merchant application they submitted goes through an underwriting process. Depending on different guidelines such as personal credit history, how long the merchant has been in business, how large/small tickets are..etc. This specific merchant was only approved to process minimum terms due to their derogatory personal credit history.
3. When the merchant went over their approved parameters, funds were held to verify the legitimacy of the transaction. Our Risk Department calls the cardholder and confirms that they did charge that particular transaction. This is also done to protect the merchant.
4. Due to the possible risk and/or fraud involved with their business type, BankCard USA Merchant Services, Inc. has the right to hold the merchant's funds up to a period of 180 days or less (please refer to a copy of your Merchant Account Agreement). Keyed businesses are considered high risk businesses because of the high ticket, (which can later evolve to higher dollar amounts of chargebacks, retrievals..etc), keyed transactions, home based business (retail, face to face, swiped businesses have less risks because the credit card is always present and swiped through the credit card terminal).
5. BankCard USA Merchant Services, Inc. is liable for any charges, returns, chargebacks that the merchant processes, under the rules and regulations of Visa/Master Card. Thus, the reason for capturing funds to protect BankCard USA Merchant Services, Inc.
Please contact us directly with any additional questions or concerns.
Thank you,
BankCard USA
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Search: Avatar - The Last Air Bende…Search profiles »
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Shows: LOST, Avatar: The Last Airbender, True Blood, Game of Thrones, Its Always Sunny, Arrested Development, ROME, Grey's ... If I was a bender, which one would I be?hmmmm air bender! NO water bender!
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took almost a decade to make and is beautiful), The Matrix, A beautiful mind, Avatar (Despite what some people may think I thought it was cool, and we are talking about the blue alien people one not the last air bender one), Inception, The Dark Knight (Saw it 5 times in the theater), The Girl
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just to name a few & I'm a sucker for mellow jazz infused hiphop beatsAvatar last air bender, Clarence, adventure time, Thee amazing world of Gumball, 90's sitcoms, Comedy Central, science, history channel, MMA, bizarre foods,Hook, Cloud Atlus, The matrix, the fountain, Hot rod, Nacho libre,
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Books: Avatar Last Air Bender, HolesMovies: Harrypotter, Hunger Games, Iron Man, Captain America , Bat man, Red Dawn, How to Train Your Dragon 1 & 2, Maze Runner,TV: Walking dead, Sword Art Online, The Fosters, Avatar Last Air Bender, How I met your mother, Switched At Birth, Melissa & Joey,
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MANGA: Naruto, Naruto Shippuden, Bleach, Death Note, Avatar: the last air-benderMOVIES: Brave, Riddick, The Hobbits, Lord of the Rings, Ghost in the Shell, Thor, Hancock, Serenity, Iron man, Avengers, Hunger Games, Van Helsing, Resident Evil, ZombieLand, Pirates of the Caribbean, Harlock, Harry
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Mahesh Babu Playing A Cameo Role In Sudheer's KKI
Superstar Mahesh Babu, who is busy wrapping up his upcoming Telugu action entertainer Aagadu, will be seen in a cameo role in upcoming Telugu film Krishnamma Kalipindi Iddarini (KKI), starring his brother-in-law Sudheer Babu. So far, Prince has only lent his voice for films like Jalsa and Baadshah and this this for the first he will be making a special appearance in a movie.
Without divulging the details about Mahesh Babu's cameo, R Chandru, who is directing Krishnamma Kalipindi Iddarini, says that he has an interesting role in it. Chandru said, "I have a very interesting role for Mahesh in the film. When I narrated it to him, he instantly agreed to do it. Moreover, Sudheer was also responsible for him to come on board. It will be an interesting cameo and his fans are going to love it."
Mahesh Babu is expected to shoot for his role as soon as he completes shooting for Aagadu. Krishnamma Kalipindi Iddarini is the Telugu remake of Kannada film Charminar, which was directed by R Chandru himself. This film marks Chandru's debut in Tollywood. Nandita is paired with Sudheer in the film, while actors such as Giribabu, MS Narayana and Kishore Das are playing important roles.


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Sleeping Dogs: Nightmare in North Point
After his girlfriend is kidnapped, Wei must fight through an invasion of hopping vampires, demons, and the ghosts of his past who are threatening to take over the city. Luckily, Wei has new moves and weapons at his disposal to make it through this nightmare.
If you're stuck in Sleeping Dogs: Nightmare in North Point, ask your fellow GameFAQs members for help.
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Are you Sergio Bondanza?Claim your profile
Publications (44)
Article: 086 The role of Excision Repair Cross-Complementation Group 8 protein in the modulation of oxidative stress and senescent-associated secretory phenotype in keratinocytes from a patient suffering from Cockayne syndrome
Article: The role of oncogenic Ras in human skin tumorigenesis depends on clonogenic potential of the founding keratinocytes[Show abstract] [Hide abstract] ABSTRACT: The role of Ras in human skin tumorigenesis induction is still ambiguous. Overexpression of oncogenic Ras causes premature senescence in cultured human cells and hyperplasia in transgenic mice. We investigate whether the oncogenic insult outcome may depend on the nature of the founding keratinocyte.We demonstrate that Ras-V12-overexpression induces senescence in primary human keratinocyte cultures, but some cells escape senescence and proliferate indefinitely. Ras-overexpression in transient-amplifying (TA)- or stem cell (SC)- enriched cultures shows that p16 levels are critical for the final result. Indeed, TA-keratinocytes expressing high levels of p16 are sensitive to Ras-V12-induced senescence whereas cells with high proliferative potential, which do not display p16, are resistant. The subpopulation, which sustains the indefinite culture growth, exhibits SC features. Bypass of senescence correlates with pRb pathway inhibition and TERT resumption. Immortalization is also sustained by activation of ERK1/2 and Akt pathways. Moreover, only transduced cultures, originating from cultures bearing SCs, induce tumors in nude mice.Our findings demonstrate that Ras-overexpression outcome depends on the clonogenic potential of the recipient keratinocyte and only the SC compartment is competent to initiate tumorigenesis.
- [Show abstract] [Hide abstract] ABSTRACT: The SKI protein is a transcriptional coregulator over-expressed in melanoma. Experimentally induced down-regulation of SKI inhibits melanoma cell growth in vitro and in vivo. MicroRNAs (miRNAs) negatively modulate gene expression and have been implicated in oncogenesis. We previously showed that microRNA-155 (miR-155) is down-regulated in melanoma cells as compared with normal melanocytes and that its ectopic expression impairs proliferation and induces apoptosis. Here, we investigated whether miR-155 could mediate melanoma growth inhibition via SKI gene silencing. Luciferase reporter assays demonstrated that miR-155 interacted with SKI 3'UTR and impaired gene expression. Transfection of melanoma cells with miR-155 reduced SKI levels, while inhibition of endogenous miR-155 up-regulated SKI expression. Specifically designed small interfering RNAs reduced SKI expression and inhibited proliferation. However, melanoma cells over-expressing a 3'UTR-deleted SKI were still susceptible to the antiproliferative effect of miR-155. Our data demonstrate for the first time that SKI is a target of miR-155 in melanoma. However, impairment of SKI expression is not the leading mechanism involved in the growth-suppressive effect of miR-155 found in this malignancy.
Article: Bmi-1 Reduction Plays a Key Role in Physiological and Premature Aging of Primary Human Keratinocytes[Show abstract] [Hide abstract] ABSTRACT: Accumulation of senescent cells contributes to the reduced regenerative capacity in aged tissues. By evaluating the molecular pathways of senescence in relation to proliferative potential of primary keratinocyte cultures from young and old healthy donors, and from young patients with inherited defects leading to premature aging, we demonstrated that p16(INK4a) is a reliable marker of both physiological and premature epidermal aging. Analysis of the expression and activity of p16(INK4a) regulators showed that stem cell depletion, reduced proliferation, and p16(INK4a) upregulation in keratinocytes derived from the chronologically and prematurely aged epidermis strongly correlate with Bmi-1 downregulation. In highly proliferative tissues, replicative and premature senescence participate in determining senescent cell accumulation. Our findings demonstrated that Bmi-1 is downregulated in human keratinocytes during both in vitro processes, in parallel with p16(INK4a) upregulation and accomplishment of clonal conversion. When premature senescence was induced by specific exogenous stimuli, concomitant Ets-1 upregulation was also observed. Moreover, Bmi-1 inhibited Ets-1-mediated p16(INK4a) upregulation. Finally, Bmi-1 overexpression reduced p16(INK4a) promoter activity and decreased protein expression in aged and diseased keratinocytes, inducing a delay of clonal conversion and an increase of cell clonogenic ability. Altogether these findings underline a key role of Bmi-1 downregulation in enforcing aging in primary human keratinocytes.
Article: Altered expression of selected microRNAs in melanoma: Antiproliferative and proapoptotic activity of miRNA-155[Show abstract] [Hide abstract] ABSTRACT: Altered expression of microRNAs (miRNAs) has been detected in cancer, suggesting that these small non-coding RNAs can act as oncogenes or tumor suppressor genes. In the present study, we investigated the expression of miRNA-17-5p, miRNA-18a, miRNA-20a, miRNA-92a, miRNA-146a, miRNA-146b and miRNA-155 by real-time quantitative RT-PCR in a panel of melanocyte cultures and melanoma cell lines and explored the possible role of miRNA-155 in melanoma cell proliferation and survival. The analyzed miRNAs were selected on the basis of previous studies strongly supporting their involvement in cancer development and/or progression. We found that miRNA-17-5p, miRNA-18a, miRNA-20a, and miRNA-92a were overexpressed, whereas miRNA-146a, miRNA-146b and miRNA-155 were down-regulated in the majority of melanoma cell lines with respect to melanocytes. Ectopic expression of miRNA-155 significantly inhibited proliferation in 12 of 13 melanoma cell lines with reduced levels of this miRNA and induced apoptosis in 4 out of 4 cell lines analyzed. In conclusion, our data further support the finding of altered miRNA expression in melanoma cells and establish for the first time that miRNA-155 is a negative regulator of melanoma cell proliferation and survival.
Article: Nonirradiated Human Fibroblasts and Irradiated 3T3-J2 Murine Fibroblasts as a Feeder Layer for Keratinocyte Growth and Differentiation in vitro on a Fibrin Substrate[Show abstract] [Hide abstract] ABSTRACT: The standard method for producing graftable epithelia relies on the presence of a feeder layer of lethally irradiated 3T3-J2 murine fibroblasts (Rheinwald and Green technique). Here, we studied a new keratinocyte culture system, which envisages the utilization of nonirradiated human fibroblasts embedded into a fibrin substrate, in cultures destined for a future clinical application. We tested this culture system using keratinocytes grown on a fibrin gel precoated with 3T3-J2 murine fibroblasts as a control. In order to evaluate the new technology, we compared the clonogenic potential and the proliferative, differentiative and metabolic characteristics of keratinocytes cultured on the fibrin gel under the two culture conditions. The results demonstrated that the proposed technology did not impair the behavior of cultured keratinocytes and revealed that cells maintained their proliferative potential and phenotype under the experimental conditions. In particular, the demonstration of stem cell maintenance under the adopted culture conditions is very important for acute burn treatment with skin substitutes. This work is a first step in the evaluation of a new keratinocyte culture system, which has been studied in order to take advantage of an additional human cell population (i.e. nonirradiated, growing fibroblasts) for future transplantation purposes in acute and chronic wounds. Additional research will allow us to attain (1) the removal of murine cells in the initial phase of keratinocyte cultures, and (2) the removal of other potentially dangerous animal-derived materials from the entire culture system.
Article: Genotype-Phenotype Relationships in Trichothiodystrophy Patients With Novel Splicing Mutations in the XPD Gene[Show abstract] [Hide abstract] ABSTRACT: Trichothiodystrophy (TTD) is a rare, autosomal recessive neurodevelopmental disorder most commonly caused by mutations in ERCC2 (XPD), a gene that encodes a subunit of the transcription/repair factor IIH (TFIIH). Here, we describe two TTD cases in which detailed biochemical and molecular investigations offered a clue to explain their moderately affected phenotype. Patient TTD22PV showed new mutated XPD alleles: one contains a nonsense mutation (c.1984C>T) encoding a nonfunctional truncated product (p.Gln662X) whereas the second carries a genomic deletion (c.2191-18_c.2213del) that affects the splicing of intron 22 and generates multiple out-of-frame transcripts from codon 731. XPD mRNA from the second allele corresponds to 20% of the total. The predicted proteins, which are longer than normal, affect the cellular repair activity but only partially interfere with TFIIH stability, suggesting that the observed changes in the C-ter region of XPD cause minor structural changes that do not drastically compromise the transcriptional activity of TFIIH. Patient TTD24PV was compound heterozygous for a typical TTD allele (c.2164C>T, p.Arg722Trp) and for a new XPD allele with a mutation that partially affects intron 10 splicing, resulting in both mutated and normal XPD transcripts (that together represent 15% of the total XPD mRNA). Compared to the previously described TTD compound heterozygotes for the Arg722Trp change, Patient TTD24PV's cells show similar level of TFIIH but increased repair activity, suggesting that even low amounts of normal XPD subunits are able to partially rescue the functionality of TFIIH complexes.
Article: Larcher, F, Dellambra, E, Rico, L, Bondanza, S, Murillas, R, Cattoglio, C et al.. Long-term engraftment of single genetically modified human epidermal holoclones enables safety pre-assessment of cutaneous gene therapy. Mol Ther 15: 1670-1676[Show abstract] [Hide abstract] ABSTRACT: Predicting the risks of permanent gene therapy approaches involving the use of integrative gene-targeting vectors has become a critical issue after the unfortunate episode of a clinical trial in children with X-linked severe combined immunodeficiency (X-SCID). Safety pre-assessment of single isolated gene-targeted stem cells or their derivative clones able to regenerate their tissue of origin would be a major asset in addressing untoward gene therapy effects in advance. Human epidermal stem cells, which have extensive proliferative potential in vitro, theoretically offer such a possibility as a method of assessment. By means of optimized organotypic culture and grafting methods, we demonstrate the long-term in vivo regenerative capacity of single gene-targeted human epidermal stem cell clones (holoclones). Both histopathological analysis of holoclone-derived grafts in immunodeficient mice and retroviral insertion site mapping performed in the holoclone in vitro and after grafting provide proof of the feasibility of pre-assessing genotoxicity risks in isolated stem cells before transplantation into patients. Our results provide an experimental basis for previously untested assumptions about the in vivo behavior of epidermal stem cells prospectively isolated in vitro and pave the way for a safer approach to cutaneous gene therapy.
Article: Keratinocyte cultures from involved skin in vitiligo patients show an impaired in vitro behaviour[Show abstract] [Hide abstract] ABSTRACT: Vitiligo depigmentation is considered a consequence of either melanocyte disappearance or loss of functioning melanocytes in the involved areas. However, it has been reported that keratinocytes in involved vitiligo skin are damaged too. Based on this evidence, we evaluated the in vitro behaviour, in life span cultures, of involved and uninvolved vitiligo keratinocytes and their expression of proliferation, differentiation and senescence markers. An additional purpose was to investigate whether vitiligo keratinocytes from depigmented skin are able to sustain survival and growth of normal melanocytes (when added in co-culture experiments), as normal human keratinocytes manage to do. Our results demonstrate that almost all involved vitiligo keratinocytes have a shorter life span in vitro than the uninvolved cells and all of them do not maintain melanocytes in culture in a physiological ratio. Modification of proliferation and senescence marker expression also occurs. Indeed, we detected low initial expression levels of the senescence marker p16 in involved vitiligo keratinocytes, despite their shorter in vitro life span, and increased expression of proliferating cell nuclear antigen and p53. This preliminary analysis of a small number of in vitro cultured vitiligo keratinocytes suggests an impaired senescence process in lesional vitiligo keratinocytes and attempts to regulate it.
Article: Piebald Trait: Implication of kit Mutation on In Vitro Melanocyte Survival and on the Clinical Application of Cultured Epidermal Autografts[Show abstract] [Hide abstract] ABSTRACT: Piebald trait leukoderma results from "loss-of-function" mutations in the kit gene. Correlations between mutation type and clinical phenotype have been reported. However, mutation classification has been mainly based on the clinical features of patients. The aim of this study was to get a better understanding of the pathogenesis of human piebaldism by establishing whether the kit mutation type may affect the in vitro survival/proliferation of patient melanocytes. Overall, the research was finalized to implement the clinical application of the autologous cultured epidermis in the treatment of piebald patients. Seven patients, who were transplanted with autologous in vitro reconstituted epidermis, showed an average percentage of repigmentation of 90.7. Six novel and one previously reported mutations were found and their postulated effects discussed in relation to the clinical phenotype and in vitro behavior of epidermal cells. Although mutation type did not impair repigmentation given by autotransplantation, it was shown to influence the survival/proliferation of co-cultured melanocytes and keratinocytes. In particular, tyrosine kinase domain mutations were found with melanocyte loss and keratinocyte senescence during expansion of epidermal cultures. Results indicate that the clinical application of cultured epidermis in piebald patients may be optimized by investigating mutation functional effects before planning surgical operations.
Article: Maurelli R, Zambruno G, Guerra L, Abruzzese C, Gellini M, Bondanza S et al.Inactivation of p16INK4a immortalizes primary human keratinocytes by maintaining cells in the stem cell compartment. FASEB J 20:1516-1518[Show abstract] [Hide abstract] ABSTRACT: Replicative senescence of human keratinocytes is determined by a progressive decline of clonogenic and dividing cells, and its timing is controlled by clonal evolution (i.e., the transition from stem cells to transient amplifying and postmitotic cells). Progressive increase of p16INK4a (inhibitor of cyclin-dependent kinase 4A) expression has been shown to correlate with keratinocyte clonal evolution. Thus, the aim of our study is to understand whether p16INK4a accumulation is a triggering mechanism of epidermal clonal evolution or a secondary event. We show that inactivation of p16INK4a, by an antisense strategy, allows primary human keratinocytes to escape replicative senescence. Specifically, p16INK4a inactivation alone blocks clonal evolution and maintains keratinocytes in the stem cell compartment. Antisense excision is followed by keratinocyte senescence, confirming that persistent p16INK4a inactivation is required for maintenance of clonal evolution block. Immortalization is accompanied by resumption of B-Cell Specific Moloney murine leukemia virus site 1 (Bmi-1) expression and telomerase activity, hallmarks of tissue regenerative capacity. In turn, Bmi-1 expression is necessary to maintain the impairment of clonal evolution induced by p16INK4a inactivation. Finally, p16INK4a down-regulation in transient amplifying keratinocytes does not affect clonal evolution, and cells undergo senescence. Thus, p16INK4a inactivation appears to selectively prevent clonal conversion in cells endowed with a high proliferative potential. These data indicate that p16INK4a regulates keratinocyte clonal evolution and that inactivation of p16INK4a in epidermal stem cells is necessary for maintaining stemness.
Article: Autologous in vitro reconstituted epidermis in the treatment of a large nevus depigmentosus
Article: Aberrant splicing in the ocular albinism type 1 gene (OA1/GPR143) is corrected in vitro by morpholino antisense oligonucleotides[Show abstract] [Hide abstract] ABSTRACT: An intronic point mutation was identified in the ocular albinism type 1 (OA1) gene (HUGO symbol, GPR143) in a family with the X-linked form of ocular albinism. Interestingly, the mutation creates a new acceptor splice site in intron 7 of the OA1 gene. In addition to low levels of normally spliced mRNA product of the OA1 gene, the patient samples contained also an aberrantly spliced mRNA with a 165 bp fragment of intron 7 (from position +750 to +914) inserted between exons 7 and 8. The abnormal transcript contained a premature stop codon and was unstable, as revealed by Northern blot analysis. We defined that mutation NC_000023.8:g.25288G>A generated a consensus binding motif for the splicing factor enhancer ASF/SF2, which most likely favored transcription of the aberrant mRNA. Furthermore, it activated a cryptic donor-splice site causing the inclusion between exons 7 and 8 of the 165 bp intronic fragment. Thus, the aberrant splicing is most likely explained by the generation of a de novo splicing enhancer motif. Finally, to rescue OA1 expression in the patient's melanocytes, we designed an antisense morpholino modified oligonucleotide complementary to the mutant sequence. The morpholino oligonucleotide (MO) was able to rescue OA1 expression and restore the OA1 protein level in the patient's melanocytes through skipping of the aberrant inclusion. The use of MO demonstrated that the lack of OA1 was caused by the generation of a new splice site. Furthermore, this technique will lead to new approaches to correct splice site mutations that cause human diseases.
Conference Paper: Inactivation of p16(INK4a) immortalizes primary human keratinocytes by maintaining cells in the stem cell compartment
Article: Permanent repigmentation of piebaldism by erbium : YAG laser and autologous cultured epidermis[Show abstract] [Hide abstract] ABSTRACT: Several surgical techniques have been proposed for the treatment of piebaldism. These procedures, however, are poorly suited for the treatment of large leucodermal lesions, can cause scars and require multiple donor sites. Recently, it has been reported that autologous cultured epidermis induces scarless repigmentation of large vitiligo lesions, using a single small donor site. To induce permanent repigmentation of large achromic lesions in patients suffering from piebaldism by means of autologous cultured epidermal grafts using a rapid, simple and non-invasive surgical procedure. Six patients with piebaldism were enrolled in this study. Achromic epidermis was removed by means of appropriately set erbium:YAG laser and autologous cultured epidermal grafts were applied on to the recipient bed. Melanocyte content was evaluated by 3,4-dihydroxyphenylalanine reaction. The percentage of repigmentation was calculated using a semiautomatic image analysis system. Autologous cultured epidermis, bearing a controlled number of melanocytes, induced repigmentation of all piebald lesions. The mean percentage repigmentation was 95.45% (2791.5 cm2 repigmented/2924.2 cm2 transplanted). Autologous cultured epidermal grafts induce permanent and complete repigmentation of piebald lesions, in the absence of scars. Erbium:YAG laser surgery is a rapid and precise tool for disepithelialization, hence allowing treatment of large piebald lesions during a single surgical operation.
- [Show abstract] [Hide abstract] ABSTRACT: To induce complete and reproducible repigmentation of large "stable" vitiligo lesions by means of autologous cultured epidermal grafts using a rapid, simple, and minimally invasive surgical procedure. Achromic epidermis was removed by means of appropriately settled erbium:YAG laser, and autologous epidermal grafts were applied onto the recipient bed. Melanocyte content was evaluated by dopa reaction. The percentage of repigmentation was calculated using a semiautomatic image analysis system. A biosafety level 3-type cell culture facility, a surgical ambulatory department, and a dermatological department in a hospital. Twenty-one patients with different types of vitiligo were admitted to the study and treated with autologous cultured epidermal grafts. Inclusion criteria were failure of at least 2 standard medical approaches; no therapy for at least 12 months; no progression of old lesions or appearance of new lesions; no Koebner phenomenon within the past 18 months; and no autoimmune disorders. The average percentage of repigmentation in 21 patients was 75.9% (1759.7 cm2 repigmented/2315.8 cm2 transplanted). Three patients showed a reactivation of their vitiligo and did not show repigmentation. The remaining 18 patients, with 43 distinct lesions, showed an average percentage of repigmentation of 90% (1759.7 cm2 repigmented/1953.4 cm2 transplanted). Under appropriate conditions, cultured epidermal grafts induce complete repigmentation of stable vitiligo lesions. Erbium:YAG laser surgery can supply a fast and precise tool for disepithelialization, hence allowing treatment of large vitiligo lesions during a single surgical operation.
- [Show abstract] [Hide abstract] ABSTRACT: Melanocytes represent the second most important cell type in the skin and are primarily responsible for the pigmentation of skin, hair, and eyes. Their function may be affected in a number of inherited and acquired disorders, characterized by hyperpigmentation or hypopigmentation, consequent aesthetic problems, and increased susceptibility to sun-mediated skin damage and photocarcinogenesis. Nevertheless, the possibility of genetically manipulating human melanocytes has been hampered so far by a number of limitations, including their resistance to retroviral infection. To address the problem of human melanocyte transduction, we generated a melanocyte culture from a patient affected with ocular albinism type 1 (OA1), an X-linked pigmentation disorder, characterized by severe reduction of visual acuity, retinal hypopigmentation, and the presence of macromelanosomes in skin melanocytes and retinal pigment epithelium (RPE). The cultured patient melanocytes displayed a significant impairment in replication ability and showed complete absence of endogenous OA1 protein, thus representing a suitable model for setting up an efficient gene transfer procedure. To correct the genetic defect in these cells, we used a retroviral vector carrying the OA1 cDNA and exploited a melanocyte-keratinocyte coculturing approach. Despite their lower replication rate with respect to wildtype cells, the patient melanocytes were efficiently transduced and readily selected in vitro, and were found to express, process, and properly sort large amounts of recombinant OA1 protein. These results indicate the feasibility of efficiently and stably transducing in vitro not only normal neonatal, but also mutant adult, human melanocytes with nonmitogenic genes.
Conference Paper: Toward gene therapy of junctional epidermolysis bullosa (JEB)
Article: Gene Correction of Integrin 4-dependent Pyloric Atresia-Junctional Epidermolysis Bullosa Keratinocytes Establishes a Role for 4 Tyrosines 1422 and 1440 in Hemidesmosome Assembly[Show abstract] [Hide abstract] ABSTRACT: The cytoplasmic domain of β4integrin contains two pairs of fibronectin-like repeats separated by a connecting segment. The connecting segment harbors a putative tyrosine activation motif in which tyrosines 1422 and 1440 are phosphorylated in response to α6β4 binding to laminin-5. Primary β4-null keratinocytes, obtained from a newborn suffering from lethal junctional epidermolysis bullosa, were stably transduced with retroviruses carrying a full-length β4 cDNA or a β4 cDNA with phenylalanine substitutions at Tyr-1422 and Tyr-1440. Hemidesmosome assembly was evaluated on organotypic skin cultures. β4-corrected keratinocytes were indistinguishable from normal cells in terms of α6β4expression, the localization of hemidesmosome components, and hemidesmosome structure and density, suggesting full genetic and functional correction of β4-null keratinocytes. In cultures generated from β keratinocytes, β4 mutants as well as α6 integrin, HD1/plectin, and BP180 were not concentrated at the dermal-epidermal junction. Furthermore, the number of hemidesmosomes was strikingly reduced as compared with β4-corrected keratinocytes. The rare hemidesmosomes detected in β cells were devoid of sub-basal dense plates and of inner cytoplasmic plaques with keratin filament insertion. Collectively, our data demonstrate that the β4 tyrosine activation motif is not required for the localization of α6β4 at the keratinocyte plasma membrane but is essential for optimal assembly of bona fide hemidesmosomes.
Top Journals
Istituto Dermopatico dell'ImmacolataRoma, Latium, Italy
Università degli Studi di Genova
Genova, Liguria, Italy
- Dipartimento di Medicina sperimentale (DIMES)


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Vibration Diagnostics
Recent vibration diagnostics applications include failure investigation of a material separation machine, heavy equipment cab noise reduction, and floor vibration survey for sensitive equipment.
Expertise in advanced vibration measurement and analysis techniques including modal testing (rap testing or bump testing), operating deflection shape (ODS) analysis, strain gage measurements, spectrum (signature) analysis, order tracking, joint time frequency (waterfall) analysis, demodulation, and finite element analysis (FEA) and state of the art equipment enables SimuTech to diagnose the root cause of noise and vibration problems. Based on a clear understanding of the problem SimuTech provides practical solutions such as specification of isolation systems, tuned mass dampers, surface damping treatments, shaft balance and alignment procedures, excitation source control, repair procedures, natural frequency detuning, and active vibration control. SimuTech's vibration troubleshooting and diagnostic service offers state-of-the-art techniques at reasonable prices with a strong commitment to customer service.


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Hey Gals and Guys of the Class of '61
We will be having a 55th Class Reunion next year
(2016) to celebrate how old we are getting and how much fun we had way back
then. The date is the weekend of
September 10th, 2016, with a Friday night gathering and a Sunday
morning kickoff breakfast. The Reunion will be held at the Fairgrounds 4-H barn (the
smell is supposed to be gone by then).
The cost will be $30.00 per person and will include a catered pork chop
or chicken meal, music, a photo booth (we hope), many of your lost friends, and
a heck of a good time for all who attend.
I am the 'official' contact person so send any questions or
other response to me at:
I hope to see you there,
The classes of 1963, 64 and 65 will be holding their Class Reunions on the 25th and 26th of June. There will be a get together at the old jail off the square Friday Night and the Class Reunions will be the next night at a Hall off Broadway!


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Breakaway: Pre-Orientation Wilderness Trip
Choose your adventure. See the sights. Experience the outdoors.
Orient yourself to Duluth-Superior and the surrounding natural beauty through Breakaway - a pre-orientation outdoor adventure experience for incoming students. Breakaway helps connect incoming students with each other, while being led by experienced student guides. The staff will help answer questions about UW-Superior, and will teach you how to safely experience the outdoors. Breakaway is designed for any level of outdoor skills and knowledge (from little to none to those with experience in the backcountry. All are welcome! No experience is necessary!
Top 3 Reasons Previous Participants Attended Breakaway:
- Opportunity to connect with other incoming students and upper classman.
- The fun and unique outdoor adventure experiences.
- Opportunity to experience part of what UWS has to offer before orientation and classes start.
Choose between Backpacking the Superior Hiking Trail or Canoeing the Namekagon River.
Backpacking the Superior Hiking Trail:
One the premier backpacking trails in the Midwest and United States, the Superior Hiking Trail, boasts over 310 miles of trail that starts south of Duluth and heads all the way to the Canadian border. The Superior Hiking Trail is known as the best long hike between the Appalachian Trail and the Continental Divide. The trail follows the ridgeline overlooking Lake Superior, and provides some of the best views of the lake. Sightings of moose and bald eagles are possible along this trip. Join us as we hike this beautiful trail and take in all the beautiful scenery!
Namekagon River Canoe Trip:
One of Wisconsin's premier canoeing rivers where abundant wildlife, such as bald eagles and otters can be found, and where a mixture of calm and swift gentle rapids are fun paddle spot. The Namekagon River has served as vital part of river travel for connecting people to the St. Croix and then onto the Mississippi River. The Namekagon River offers fresh experiences for all. Join us as we canoe this beautiful river and look for the abundance of wildlife that call this river home!
Dates for all Trips: Friday, August 26 - Wednesday, August 31
Cost: $300 includes all necessary gear, meals, and transportation
Please contact Superior Adventures with any questions at 715-395-4604 or e-mail firstname.lastname@example.com


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Estimated Standard Shipping Delivery Dates
Active kids love to grasp the handles of these inflatable hop balls and bounce, bounce, bounce! Made in Italy, our hop balls are crafted from strong, heavy-duty vinyl, and come with one valve and two plugs. Kids will have a ball bouncing around on these bouncy balls. Maximum weight limit is 160 lbs.
Our foot pump (sold separately) makes ball inflation easy.
• Inflatable ride-on hop balls
• Strong, heavy-duty vinyl
• Come with one valve and 2 plugs
• Maximum weight limit 160 lbs.
• Foot pump (sold separately) makes ball inflation easy
For ages 5 and up; maximum weight = 160 lbs.
Red Bigger Hop Ball 21-1/2"H
Blue Biggest Hop Ball 26"H
Hop Balls, Inflatable Heavy-Duty 26"H Vinyl Hop Ball, in Blue
$29.98In Stock
Hop Balls, Inflatable Heavy-Duty 21-1/2"H Vinyl Hop Ball, in Red
$22.98In Stock
Dual Action Foot Pump
$11.98In Stock
Send a quick note to friends and family members to let them know your child would love this!
Dear _______:
My child has been so good this year! I know the holiday would be filled with love and laughter if they received this as a gift.
This product is not available!


text:
Household Durables
Company Overview of M/I Homes, Inc.
Executive Profile
Michael P. Glimcher
Independent Director and Member of Nominating and Governance Committee, M/I Homes, Inc.
|Total Calculated Compensation
|This person is connected to 3 Board Members in 3 different organizations across 2 different industries.
See Board Relationships
Mr. Michael P. Glimcher served as the Chief Executive Officer and Vice Chairman of the Board of WP GLIMCHER Inc. from January 15, 2015 to June 20, 2016. Previously, Mr. Glimcher served as the Chief Executive Officer since January 10, 2005 and Chairman of the Board since September 6, 2007 of Glimcher Realty Trust. Prior to this, he served as the President at Glimcher Realty Trust from 1999 to September 2007 and its Executive Vice President since 1991. He also served as ...
Corporate Headquarters
3 Easton OvalPhone: 614-418-8000
Columbus, Ohio 43219
United States
Fax: 614-418-8080
Board Members Memberships
Independent Director and Member of Nominating and Governance Committee
EducationThere is no Education data available.
Other Affiliations
Annual CompensationThere is no Annual Compensation data available.
Stocks OptionsThere is no Stock Options data available.
Total Compensation
The information and data displayed in this profile are created and managed by Capital IQ, a Standard & Poor's company. Bloomberg.com does not create or control the content. For inquiries, please contact Capital IQ directly by clicking here.
|Keith E. Bass
|Chief Executive Officer, President, Director and Chairman of Land Sub-Committee
WCI Communities Inc.
|Roger A. Cregg
|Chief Executive Officer, President and Director
AV Homes, Inc.
|Allan P. Merrill
|Chief Executive Officer, President and Director
Beazer Homes USA Inc.
|Eric Thomas Lipar
|Chairman of The Board and Chief Executive Officer
LGI Homes, Inc.
|Compensation as of Fiscal Year 2015.
Sponsored Financial Commentaries
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- Report: #1017328
Report - Rebuttal - Arbitrate
Complaint Review: Security Solutions Today
Security Solutions Today17729 Mitchell North, Irvine - Orange County, California United States of America
Security Solutions Today aka First Alert Solution, Dexter Wilson Scams Appt Setters - Irvine - Orange County, California
*UPDATE EX-employee responds: He got me too!
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This report was posted on Ripoff Report on 02/20/2013 10:50 AM and is a permanent record located here: http://www.ripoffreport.com/r/Security-Solutions-Today/Irvine-Orange-County-California-92614/Security-Solutions-Today-aka-First-Alert-Solution-Dexter-Wilson-Scams-Appt-Setters-Irvi-1017328. The posting time indicated is Arizona local time. Arizona does not observe daylight savings so the post time may be Mountain or Pacific depending on the time of year.
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#1 UPDATE EX-employee responds
He got me too!
AUTHOR: KJ Dorsey - (United States of America)
SUBMITTED: Wednesday, February 20, 2013POSTED: Wednesday, February 20, 2013
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Northwest native songwriter and activist standing for and singing about cleaner oceans, a healthier planet, equal people, and love for all.
"At WSU Vancouver's annual Research Showcase on April 14, local singer and songwriter Adam Dvorak belted out his song "Stand Up, Scream Out" to the gathered students and to anyone watching on the Internet."
"Adam's music is organic, passionate and motivating. It promotes civic engagement and social awareness."
© 2016 eMinor Incorporated
All third party trademarks are the property of the respective trademark owners. ReverbNation is not affiliated with those trademark owners.
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text:
Helmhaus Hotel is Zurich's smallest and most centrally located city hotel. Hotels 24 rooms feature high-standard design furniture. Sound-proof windows ensure privacy and tranquility. The hotel is non-smoking since 2009 and a complimentary high-speed WLAN internet access service is available to all guests. In July 2011 the Hotel Helmhaus received the valued Quality seal of the Swiss Tourism Board for the second time in a row.
The 24 rooms–all equipped with bath or shower–feature a mini-bar, a phone, air conditioning, hairdryer, TV, safe and a desk with a computer link. All the rooms are non- smoking and feature free wireless access to the internet.
Start your day at the Helmhaus breakfast buffet. The selection of products on the buffet has been chosen with utmost care.
High-speed wired and wireless Internet access is complimentary. Business-related amenities at this 4.0-star property consist of a 24-hour business center and limo/town car service.


text:
At Adrenaline Paintball we service what we sell and we stock many of the parts you're going to need right in our store. With a very few exceptions we'll fix youMore
$1 mil. - $5 mil.in Revenue
10 - 20EmployeesContact people at this company
1350 Trafalgar StreetLondonOntarioN5W 1W6Canadaview map
(519) 453-0434(519) 453-5860Fax
Enterprise_Iteration-NT.1579[03] SEIDXWK-35


text:
Gift Premium Subscription To Brewing_Master
Join Date:01-03-2011
Last Activity:11-20-2015 8:30 PM
1 Likes on 1 Posts
- Single
- Westminster
- Colorado
Posted in thread: HomeBrewSupply Amcyl Brew Kettle w/ 3-Piece Ball Valve Giveaway! on 07-28-2015 at 05:28 AM
entry plox
Posted in thread: Spike Brewing 12.5 Gallon Conical Fermenter Giveaway! on 01-06-2014 at 07:04 PM
Looks tasty. In plz
Posted in thread: Rebel Brewer Giveaway! on 11-23-2013 at 11:29 PM
oo lala me likey
Posted in thread: NorcalBrewing Solutions Giveaway! on 10-09-2013 at 04:35 AM
me want plox
Posted in thread: MoreBeer.com Summer Giveaway! on 08-29-2013 at 10:52 PM
me me me
Posted in thread: Brewer's Hardware 15 Gallon Stainless Steel Conical Fermenter Giveaway! on 08-15-2013 at 01:44 AM
in plz
Posted in thread: StainlessBrewing.com Summer Giveaway! on 06-25-2013 at 06:44 PM
Already made a few major purchases from stainless brewing but I still have a definite need for
those prizes. Throw my name in!
Posted in thread: "ARRIBA!! Mexican Chocolate Porter" on 04-17-2013 at 10:47 AM
If there is no tested visual change in gravity then the beer is stable enough to bottle. But
from experience 1016 is about where a dark beer with that high of an OG should finish at.
Posted in thread: "ARRIBA!! Mexican Chocolate Porter" on 03-23-2013 at 11:49 AM
I would say try raising temp first, maybe try rousing the yeast with a quick swirl.Agreed, also
monitor (check gravity in the next 3-5 days) to make sure there is no further gravity change.
If no chan...
Posted in thread: Keezer Temp probe location on 03-01-2013 at 11:28 AM
I would just let it dangle without being in contact with anything.When the probe is in liquid
the temperature transfer of the liquid is delayed (well insulated) so the fridge/freezer will
run until th...


text:
Mar 02nd 2015
Delray to Fete Bacon, Bourbon
Pairings, tastings, seminars, live blues and more. . . Read More
Pairings, tastings, seminars, live blues and more. . . Read More
Delray Beach has been nominated for America's Happiest Seaside Town. Read More
City Watch: Boca offers no clear report yet on pension changes, the options are lining up to settle the Mizner Trail issue, and Delray should pass its land use regs tonight. Read More
The Spady Museum honors Delray heritage, Mizner Park hosts a health and wellness bonanza, and modern masters of painting exhibit in Fort Lauderdale. Plus, Maroon 5, Richard Lewis, Miami City Ballet and more in your week ahead. Read More
An evening with Moonshine Nation author, Mark Spivak. Read More
Artists take to the streets in Lake Worth, the Plumosa School books a genre-hopping virtuoso, and Palm Beach Opera stages a world premiere. Plus, Alice Cooper, Lang Lang, Contra-Tiempo and more in your week ahead. Read More
Get in touch with your mind, body and soul at one of these health fairs. Read More


text:
The 21-year-old Harrison, who has yet to back up his early promise and languishes 102nd in the rankings, is 10-17 this year and still seeking his first career ATP title.
American Brian Baker, who reached the fourth round of last year's Wimbledon after returning from a nearly six-year layoff due to injuries, was also given a wild card into the year's final grand slam.
Baker, who is currently ranked 185th but reached 52nd in 2012, returned to action this week after suffering a knee injury at the Australian Open in January.
Australia's James Duckworth and France's Guillaume Rufin represented the only international players named as wild cards.
Americans Rhyne Williams, Bradley Klahn, Rajeev Ram and 2013 U.S. Under-18s champion Collin Altamirano rounded out the wild cards for the Aug. 26-Sept. 9 tournament at Flushing Meadows.
- Sports & Recreation


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Gurman-Billings Family Tree - 30062015 (1) - 01072015 (1) - 51027031 - 03072015
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In this family tree (first 100 people):
Richard Bumford Barnes
Ronald Leslie Billings
Emmilie Lucie Bono
Robert Alfred Bray
Patricia Margaret Campbell
Elsie May Carr
Elizabeth Chalk
George Chalk
James Chalk
Joseph Chalk
Lomes Chalk
Mary Chalk
Sarah Chalk
Emma Eliza Chalk
Elizabeth Chapman
Caroline Amelia Chitts
James Collins
Mary Collins
Annie Agnes Dawson
Emma Dawson
Francis J Dawson
Isabella Alice Dawson
Richard Dawson
Richard Henry Dawson
Rose A Dawson
Mary J Dawson
Emma Catherine Dawson
Emma Catherine Dawson
Mary Ann Anne Dawson
Maria Dellequin
August Eichorn
Kathleen Ethel Eichorn
Jane Rose Elmes
Edward James Foster, Sr.
Dora Foster
Gordon Francis
Henry Francis
Henry Francis
Robert Wise Francis
Rose Francis
William Francis
Rhoda Francis
Grace Francis
Annie Goodwin
Anne Grimes
Anne Elizabeth Grubb
Mary Gurman
Charles Henry Harris
George Jarvis Harris
George Jarvis Jonathan Harris
George Robert Grubb Harris
Harriet C Lilly J Cecilia Julia Harris
Henry Richard Harris
Jonathan G Harris
Letittia Harris
Louisa Amelia Francis Rachel Molina Harris
Susan Margaret Lucretia Harris
Elizabeth Harris
Catherine May Harris
Elizabeth Heather
John Lewington
Georgina Annie Elizabeth Ethel McGowan
John William McGowan
Emily J McGowan
George Miles
Priscilla Miles
Richard Missingham
Jenny Jane Missingham
Jane Newman
William Newman
Sarah Newman
Jane G Percy
Denise Poulain
Gerges Emile Poulain
Michel Poulain
Roger Poulain
Philomene Marie Joseph Poulain
Elizabeth Rooke
James Charles Rooke
Jane Rooke
Jane Rooke
Jessie C E Rooke
John Rooke
John Rooke
Mark Rooke
Mary Rooke
Richard Rooke
Richard Henry Rooke
William Thomas Rooke
Esther Rooke
Jessica Caroline Eleanor Rooke
William Arthur Rowe
Noah Shepherd
Elizabeth Stapley
Charles Turville Wilson
Hardingham George James Wilson
Mary Anne Wilson
Stanley William Wilson
Amy Evelyn Wilson


text:
To the Editor:—
In the caption to the caricature reproduced on the cover of The Journal for Feb 7, the statement is made that "The explanation for the combination of British gentlemen with oriental servants and surroundings is no longer known." There is no date visible on the aquatint, but unless I am mistaken the explanation is this:On the saddle on the horse can be seen the words "Ich Dien." This means "I serve" and is the motto of the Prince of Wales. The gentleman mounting the horse has three feathers on his hat and these are also part of the insignia of the Prince of Wales. There was one Prince of Wales who had a liking for oriental surroundings and was responsible for the construction of the Pavilion at Brighton which is completely oriental in appearance.This particular Prince of Wales became George IV in 1820, but was
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Jill Tydor, Contributing writer
Chip Somodevilla/Getty Images
Janet Yellen is getting closer to raising interest rates.
The Chair of the Federal Reserve signaled that a rate hike this year is still on the table.
"I believe the case for an increase in the federal funds rate has strengthened in recent months," Ye...
John Moore/Getty Images
The doctor who has produced the only public medical record about Donald Trump during his presidential campaign reportedly said he spent only five minutes writing it.
Dimitrios Kambouris/Getty Images
The 2016 MTV Video Music Awards happen Sunday. But let's be honest, the VMAs are more about jaw dropping moments than music videos and Moon Man trophies.
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Total Politics Top Ten Blogs
The polling for the Total Politics top blogs have opened. You can vote by emailing email@example.com with your top ten: rules can be found here. Be sure, after you vote, to share who you voted for. I voted as follows:
1. Though Cowards Flinch (obviously)
5. Don Paskini
7. Obsolete
10. Chicken Yogurt
There are a few absences and trends which deserve explanation. Firstly, Miljenko's blog is not on the list; despite some really good essays recently, especially this one on anti-politics which was thought provoking even if it gave some grounds for challenge, I haven't been following it as often as I should.
Larger sites tend to come further down the list than reflects how much I use them – ChickYog, for example. Two huge blogs, Liberal Conspiracy and Pickled Politics are completely absent even though I use them more than almost any other blog. The reason for this is simple; the smaller blogs deserve a bigger shout out, I think. Everyone will vote for LC – and so they should!
The only potential exception is AVPS, which is actually a very popular blog and ranked highly the last time around. I don't think any other blog (saving Paul's Bickerstaffe Record) does quite so much for the discussion of socialist theory and politics on the internet.
So many blogs I couldn't include, because I only have ten votes; Socialist Unity being an obvious one, the Daily Maybe and Bob from Brockley too. Collectively these form my most read blogs, or the ones I utilize most (e.g. Obsolete's SepticIsle for his weekend round-ups). I hope a free link from TCF makes up for it guys!
July 17, 2009 at 3:04 pm
Thanks for that (almost) endorsement comrade!
July 17, 2009 at 6:24 pm
Cheers very muchly comrade
July 18, 2009 at 9:45 am
My vote is for Don Paskini.
July 19, 2009 at 8:43 pm
Cheers very much for the endorsement! 😀
July 21, 2009 at 10:13 pm
Thanks, Dave, spirits need lifting at moment in Calder Valley…..
July 22, 2009 at 10:26 am
The whole team at the Bickerstaffe Record is grateful for vote of confidence of all tat TCF, and hereby promises to be more properly leftwing over the next year, except on the occasions when we need to compromise our beliefs from a mix of cowardice and unenlightenment.
September 22, 2009 at 8:24 pm
What about The Prometheus Learnings?! (www.prometheuslearnings.blogspot.com). I love those posts.
July 25, 2009 at 4:25 pm


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PGY1 Pharmacy Residency – Aurora BayCare Medical Center Green Bay, WI
Kristin Cannon, R.Ph.
Sr. Director Pharmacy Operations Kristin received her B.S. in Pharmacy from the University of Wisconsin. Kristin is the Sr. Director of Pharmacy Operations for Aurora Health Care-North, which includes the 6 hospitals north of the Milwaukee area. Kristin co-precepts the administration rotation at Aurora BayCare Medical Center and is a preceptor in the PGY-2 Health System Pharmacy Administration program.
Julie Davis, PharmD, BCPS
Specialty Pharmacy Coordinator, Centralized Order Verification Julie received her Pharm.D. from the University of Wisconsin-Madison. She co-precepts the Centralized Order Verification (COV) rotation for PGY-1 pharmacy residents from Aurora West Allis and Aurora Baycare Medical Centers and is the primary preceptor for Concordia University of Wisconsin's Doctor of Pharmacy students in elective COV rotations. Additionally, she has involvement with PGY-1 (Metro, Administration) and PGY-2 residents (Oncology, Critical Care, Informatics, Cardiology, Administration) in their longitudinal staffing rotation which includes COV.
Jennifer A Esch, PharmD, BCPS
Clinical Pharmacist Jennifer completed her undergraduate work at Michigan State University with dual Bachelor's degrees in Business Management and Education. She obtained her Doctor of Pharmacy degree from the University of Arizona. She currently precepts the Medical/Surgical Clinical and Hospitalist required rotations at Aurora BayCare Medical Center in Green Bay.
Julie L. Kindsfater, PharmD, BCPS
Drug Policy, Formulary Coordinator, Sr. Julie received her Pharm.D. from the University of Minnesota – Twin Cities. She completed a PGY-1 Pharmacy Practice Residency at Froedtert Memorial Lutheran Hospital in Milwaukee. Julie co-precepts the drug policy rotation and assists residents with medication safety projects. She chairs the Aurora Medication Safety Committee and recently completed coursework in Patient Safety Leadership through the College of Medicine at the University of Illinois – Chicago.
Holly Kaminski, R.Ph
Clinical Pharmacist Holly received her B.S. in Pharmacy from the University of Wisconsin-Madison. She joined Aurora Health Care Metro Region in 2000 and moved North to Aurora BayCare Medical Center in 2002. Holly's primary focus is central pharmacy distribution and order verification for BayCare Medical Center. She precepts the Central Order Verification (COV) rotation in collaboration with Aurora Health Care's Metro Verification team
Jessie Lawton, Pharm.D., BCOP
Clinical Pharmacist Jessie received her BS from Marquette University and her Pharm.D. from the University of Minnesota. She completed a hematology/oncology specialty residency at NorthShore University Health System in Evanston, IL. Jessie practices at Aurora BayCare Medical Center and precepts for the PGY1 oncology rotation and PGY2 oncology residency.
Tracy Mingori, Pharm.D.
Clinical Pharmacist Tracy received her B.S. in Pharmacy from Ferris State University and her Pharm.D. from the University of Florida. She completed a PGY-1 Pharmacy Practice Residency and PGY-2 Critical Care Residency at Spectrum Health in Grand Rapids, Michigan. Tracy currently co-precepts the Adult Medicine and Critical Care rotations at Aurora BayCare Medical Center.
Lee Skrupky, PharmD, BCPS
PGY1 Pharmacy Residency Program Director – Aurora Baycare Medical Center
Clinical Coordinator Lee received his PharmD from the University of Wisconsin-Madison. He completed a PGY1 Pharmacy Practice Residency and a PGY2 Critical Care residency at Barnes-Jewish Hospital in St. Louis, MO. Lee co-precepts the critical care rotation and infectious diseases elective.
Lisa Streu, R.Ph
Clinical Pharmacist Lisa received her B.S. in Pharmacy from the University of Wisconsin-Madison. She has been practicing at Aurora Bay Care Medical Center since 2005 and co-precepts the oncology rotation.
Jeff Waise, PharmD
Director Inpatient Pharmacy Jeff Waise has been the Pharmacy Director at ABMC since 2008. He is a PharmD graduate of University of Michigan with almost 20 years of management experience. Under Jeff's leadership, ABMC has gone through 3 successful Joint Commission surveys, converted to an All Meds Model, implemented automation, and converted to Epic. Additionally, he has lead several successful clinical interventions to include parenteral nutrition, warfarin dosing and counseling, and transitions of care. Jeff is passionate about medication safety and continued process improvements.


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Hi, I'm trying to change the size of the Menu nav items on my site without changing the size of all fonts on the page.
I'm using the mystique theme if that matters.
The site is at http://www.psspoilers.com - as you can see, the menus are overflowing the page as new items are added.
I was able to change all font sizes on the page by messing with this:
body{background:#000 url(images/bg.png) repeat-x center bottom;font-size:13px;font-style:normal;color:#4e4e4e;}
But I'd like to just change the size of the font in the menus at the top.
Thanks for your help,


text:
- Report: #464917
Report - Rebuttal - Arbitrate
Complaint Review: Dell DFS Dell Financial Services
Dell DFS Dell Financial ServicesPO Box 81577 Austin, Texas U.S.A.
Dell, DFS, Dell Financial Services Dell 0% APR Fraud Austin Texas
*Author of original report: update
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Suddenly, I think almost after 3 years, I get a call from a collection agency saying that I owe DFS over $200. Only then I found out what had happened to my account, the amount they had told me over phone was falling short of the total value of the laptop by something like 55 or $60. I paid a portion of the remaining amount which was left over for the laptop (the Dell guys do amazing math!!.
Additionally, the time was not 2 years as they had told me and calculated the monthly payments for -- it was 1 1/2 years and so interest and penalities were levied at that time totaling over $200. After calling and spending more than 3 hrs on the phone I got one decent guy from DFS who was willing to look into the problem. Brad gave me his personal extension number and told me he will get back to me after reviewing the old documents since there was clearly an error somewhere. (by this time I had changed my address with DFS 3 times and still Brad told me they were sending the details to my older address - he finally managed to change the address at this time) - unfortunately, when I tried to contact Brad at his extension again after some time had elapsed (and I got no call) - the name in the voice mail went to someone else. I left a message - but no response...
At this point I called some more to try get some info and finally gave up, since I wasnt getting any call back/info from them.
Now I get a call from another collection agency / attorneys saying that I owe them $575 - for breach of contract etc. and willing to settle for $475. I am outraged by their attitude and ethics - this guy from the collection agency/attorney - said it was probably their fault and was willing to reduce 100$ and settle for $475 instead... I have no idea why I should pay fr the collective mistakes of DFS.
1) first their employee - setup a wrong monthly payment for me
2) they never updated my address even after repeatedly calling them so i never got any idea of my account till last Jan
3) they refused to stop escalating charges/ get back with any reasonable information about what was going on to resolve the issue -- whenever I talked to them - they kept saying that I owe that money and I have to pay it!
I even offered to buy a new laptop from them that will cover the money I owe them(I do like their hardware, unfortunately)
There is nil after service , or customer service after getting the money.
I pay monthly bills larger than the laptop and I find it outrageous that I should pay them for their mistake. I now know how this country is in such deep problems - thanks to unethical business practices aimed at making money through devious means. If someone from dell was/is willing to listen to the customer and do something about it - this could have been avoided and they might not have lost not just one customer but I am going to petition my friends and former corporate(university) employer to stop buying from Dell.(incidentally, I was instrumental in getting them to get computers from Dell for the past 6 years I was with them)for their business ethics are extremely devious and fraudulent.
shrewsbury, Massachusetts
This report was posted on Ripoff Report on 06/25/2009 11:21 AM and is a permanent record located here: http://www.ripoffreport.com/r/Dell-DFS-Dell-Financial-Services/Austin-Texas-78708-1577/Dell-DFS-Dell-Financial-Services-Dell-0-APR-Fraud-Austin-Texas-464917. The posting time indicated is Arizona local time. Arizona does not observe daylight savings so the post time may be Mountain or Pacific depending on the time of year.
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#1 Author of original report
AUTHOR: Psr - (U.S.A.)
SUBMITTED: Tuesday, July 14, 2009POSTED: Tuesday, July 14, 2009
efforts of two brilliant employees from Dell: one Mr.Joseph and DFS: Ms.Per
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text:
Skip to comments."Stephen Pidgeon in imminent danger of arrest by Homeland Security," Says Email
Posted on 03/25/2009 11:54:34 AM PDT by unspunEdited on 03/25/2009 2:01:54 PM PDT by Admin Moderator. [history]
click here to read article
No - people standing up to the usurper while other morons are more interested in the Final 4 or Americanm Idol.
I guess one could say they are "Homing" in on Pidgeon because he is a "Carrier" of bad news.
The RFID chip was in his passport, not implanted in his skin or body.
Passports with RFID chips were issued beginning in 2005/2006.
Donofrio's driver's license had expired while he was out of town and he assumed he would need identification in order to present/file legal documents. So he took his passport with him.
Comments like that bring ss agents to your doorstep...Careful.
No, I always use my name as a subject in emails I send, just like this cat...
Subject: Stephen Pidgeon in imminent danger of arrest by Homeland Security
...don't all sane people?
I hadn't heard of this guy, but it seems he's a birther attorney. If Bambi has him arrested, that would give tremendous cred to the birther thesis, now wouldn't it? Doesn't make sense.
It's been 2 hours, is he dead, arretsed, at home?
(Update: 3/25, 4:29pm CT) Email from Stephen Pidgeon:
Yes, this is true. We are currently being tailed by Snohomish County Sheriffs and the Everett Police Dept. They are also tracking my associates.
Question had been emailed to Pidgeon:
Stephen, is the report of the black suv from HS and the men in camo true, then?
An email from your other account was sent to Carmen, who sent it out to others including me.
Please reply as soon as convenient, thanks. Blessings.
PS: Carmen also relates that another citizen's grand jury colleague has seen the same thing in his neighborhood and the son of another was arrested peculiarly, last night or this morning. — ??
(Update: 3/25, 4:20pm CT) At 3:50pm, I received an email from another address used by Stephen Pidgeon. In this message, also apparently from Mr. Pidgeon, with a valid looking signature footer, merely states, "I have lost my primary email account for purposes of making contact. I am checking on the changeup now."
I also spoke with Carmen, who states that another associate of Pidgeon in his soon to be engaged, Citizen's Grand Jury investigation of Barack Obama and his eligibility as Commander in Chief has also seen black SUV(s) very recently, in his neighborhood. Also, the son of another on this project was arrested this morning or last night, for something to do with his license plate (out of state). He was arrested after he would not answer questions, this after his Miranda rights were apparently read, and was assigned a $50,000 bail.
I didn't threaten anyone.
They do the driving not me. Sorry for the bad post
What Stephen Pidgeon must avoid at all cost is to be caught with Orange stains on the front of of his drawers, then Homeland Security will know he eats Cheeto's and plays with himself.
Hey, I'm just looking out for you. I know what you meant.
You are assuming a modicum of respect for the actual laws.
From B. Hussein Obama and Janet Napolitano?
What where you thinking?
Yeah, well, I forgot. The law and the Constitution no longer matter. I have to get used to this.
My bad.
Thanks for the update.
for something to do with his license plate (out of state). He was arrested after he would not answer questions, this after his Miranda rights were apparently read, and was assigned a $50,000 bail.
I don't think that rates a $50k bond,,,no way,,,
Must be more charges...
Sane people? Most sane Americans did not expect to have a Kenyan-born, marxist muslim usurper spending a trillion a week to bankrupt the country.
These people will stop at nothing especially if Pidgeon has very sensitive information that could expose the usurper.
How do you know that?
Roger that. I appreciate it also. Big thanks.
Semper Fi
pardon me for jumping in here...(all the way from Australia)...but the answer is this:
Because it was discussed on Plains Radio with Pidgeon. He supposedly has the materials in safe places including safe deposit boxes.
Pidgeon supposedly has some very good information about Obama.
I guess the Kenyan is taking the grand juries seriously.
Was it Leo who did the research and found the grand juries and the source was the writings of Scalia?
"Please tell me you were being sarcastic..."
They are their own worst enemies.
Supposedly? Do you expect us to take this seriously? It's more bogus b.s.
Pidgeon has a track record as an attorney who led the Broe v. Reed case. You appear to be building a good track record as a troll.
Buh bye...
Why does this smell like API? Promises, promises.. the secret whatever is always right around the corner.
No. The only specter I know on FR is Darks...
I was just kidding. Phil Spector is on trial for murder. He has had a long history of holding women hostage in his home by putting guns to their heads and scaring them half to death. The jury should begin deliberating today or tomorrow.
He is a legendary music producer from times past and so I thought everyone had heard of him. Your comment of "Don't sweat it if I point a gun at your head. Usually I only go so far... " reminded me of him, so I couldn't help but ask!
It smells exactly like API. Constant promises, no information.
Sweet justice.
;^P I know...
Thanks for the set-up to tease Darks...
Gee, I wrtote to Steven and asked how I could apply to be on this nationa grand jury. He sent an e-mail with instructions, but I decided I should not be a party to this since I have been so oyutspoken against this lying affirmative action DEMOCRAT fraud.
Sure does. Besides, HS doesn't wear camoflauge.
I'll be updating again, just a few more details at this point.
Cool. :-)
And then they didn't. The black helicopters must have gotten you, as well.
Disclaimer: Opinions posted on Free Republic are those of the individual posters and do not necessarily represent the opinion of Free Republic or its management. All materials posted herein are protected by copyright law and the exemption for fair use of copyrighted works.


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Hi, folks. I've got a contact form set up with a bunch of questions in it that new clients use to answer questions about what they want on their new site. However, it takes some time and I'd like to generate a new form for each user (so they don't use the generic form). This will let them save their forms. Is there any way to do this?
Does CF7 even allow this? If so, how do we generate individual version of
the form and let users save them as they go so they don't have to fill in the whole form in one go?


text:
Leaders say it takes 100 to 120 thousand dollars to run an individual club--money they don't have. The Boy's and Girl's Clubs in Frenchtown, Ft. Braden, and another in Miccosukee will all close.
Samuel Streit with the Boys & Girls Clubs of the Big Bend said, "In the last few months, as things have tightened up, we've known we had to make a decision. We don't want to make these decisions like this, but we have to--to be responsible for the future of the organization."
The Boy's and Girl's Club says it is working to accommodate parents and children who are a part of the local clubs.


text:
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The KCU website is managed by the digital media team. If you have a question about the website, please contact:
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text:
The state health board is holding a hearing Monday on a proposal to limit medical marijuana providers to just five patients. It could affect dispensaries that have sprouted up to serve a growing number of patients across Colorado.
The board has scheduled seven hours to take testimony in a room that holds about 450 people.
Colorado voters allowed the use of medical marijuana in 2000 by passing Amendment 20.
The board voted to limit caregivers to five patients in 2004, but a Denver judge threw that out three years later because the limit was enacted without a hearing.


text:
The 2011 Major League Baseball First-Year Player Draft is just five days away and there is still a lot of confusion at the top of the draft.
Pittsburgh holds the No. 1 overall pick and they have their work cut out for them.
There are no less than three guys who can make a case for being the top pick, including UCLA starting pitcher Gerrit Cole, Virginia starting pitcher Danny Hultzen and Rice third baseman Anthony Rendon.
But despite all the confusion at the top, this draft class is stacked with talent, particularly pitching talent.
The talent in this draft comes mostly from the college ranks, but there are some stud high school players to be on the lookout for as well.
The leader of the high school pack is Bubba Starling, who happens to be the best athlete in this draft and offers more upside than any other player in this class. His commitment to the University of Nebraska and big bonus demands could push him down the board a little bit, but he has the potential to be a legitimate five-tool player.
We will have the latest news, notes and information to get you all set for the draft next Monday night.


text:
full size
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Strapless, flowy and ethereal, this dress has classic screen-star appeal. It's made from our drapey crinkled silk chiffon and has a fitted, figure-flattering bodice with a fuller skirt that beautifully skims the floor. Crisscrossing panels form a curve-enhancing slightly dropped waist, making it a style that works well on all body types (translation: very happy bridesmaids).
Size & Fit Details
Ava long dress in silk chiffon
Rated 1 out of 5 by 1 reviewer.
Rated 1 out of 5 by Bren Zipper malfunction I originally loved the dress, it fit true to size and looked amazing on. However, those feelings were replace by complete disappointment and embarrassment when the zipper broke while I was at a friend's wedding. I was at the reception about to sit down for to dinner when I felt the zipper brake in the middle exposing my entire back. In a panic I left the reception and headed to the hotels concierge service for help. With the sewing kits they provided I was able to sew myself into the dress. I returned to the reception an hour and half later missing the bride and grooms first dance, speeches and other important moments. Over all I was disappointed with the zipper. Thank you, Brenda December 29, 2014
1-1 of 1


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I'm a doctor. The real issue isn't Hillary Clinton's health – it's that she might winCeline GounderParkinson's, seizures, sedatives: wild rumors about the Democratic candidate's health signal fear among Trump supporters that they're heading for defeat
The hacking of the actor's website is symptomatic of the culture we've built. If you want to take a stand, more than a tweet of sympathy is required
Lawsuits can destroy media organizations. Whatever you think of the stories, the prospect of journalists being silenced by a wealthy elite is deeply troubling
Politics is killing mothers in TexasJessica ValentiThe state's maternal death rate is the highest in the developed world. Those denying women reproductive healthcare on ideological grounds are to blame
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I didn't have one until I was 47. It helped me through turbulent times – but now it is time for us to part ways
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Facing my fear: being in public as a woman for the very first timeChelsea E ManningI'd long known I was a woman. But it was the height of 'Don't Ask, Don't Tell', so I hid my true self. Then, one day on leave, I decided enough was enough
There wasn't a future for me back in Kuala Lumpur. But when I met an American man, I found a country where my background was not an obstacle
what would a feminist do?
This week, Jessica Valenti is joined by Feminist Fight Club author Jessica Bennett to discuss how to navigate difficult employers and "bropropriator" colleagues
How do you feel about the word 'feminist'? On this week's episode, we dissect our reactions to the F-word
Even among women who describe themselves as supportive of women's right, the term 'feminist' can be contentious. How do you define it?
How we can better engage men and boys to prevent gender-based violence? Jessica Valenti talks with Jamil Smith of MTV News and Dr Dorothy Edwards
Editorial: Politicians have advanced numerous reasons to ban the swimwear at the seaside. They are mistaken
Editorial: Hundreds have died since the new president, Rodrigo Duterte, announced a crackdown on crime. He likes it that way. The rest of the world should not be intimidated
Editorial: Britain voted to leave the European Union without a detailed plan. Now the EU is also realising that life for the 27 will not get any simpler
columnists & contributors
Jill AbramsonJill AbramsonThere's a lot to like about the alternatives to Hillary Clinton and Donald Trump. But sticking to your Green principles could, like in 2000, deliver a nightmare
Lucia GravesLucia GravesThe would-be president is harnessing nationalism and bigotry. Like the former Ukip leader, he is more interested in channelling anger than he is in governance
Steven W ThrasherSteven W ThrasherGreg Tate: the flyboy goes back to the futureFrom Michael Jackson's nose to the righteousness of Jean-Michel Basquiat, Tate has been analysing culture for over 30 mind-expanding years. He talks philosophy, Afrofuturism, and how black artists defy boundaries
Thomas FrankThomas FrankCome November, Clinton will have won her great victory – not as a champion of working people's concerns, but as the greatest moderate of them all


text:
Just now installed Wordfense.
However, I already have the following plugins installed:
1) Visitor Maps and Who's Online-http://wordpress.org/plugins/visitor-maps
2) Limit Login Attempts-http://wordpress.org/plugins/limit-login-attempts/
3) IP Filter-http://wordpress.org/plugins/ip-filter
My questions are:
1) Can I deactivate/delete the above mentioned plugins as I see Wordfense does the job for these plugins?
2) In the "IP Filter" plugin I have many blocked IPs. How do I add these IP in Wordfense block IP list?