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Publications (69)
Article: Bone morphogenetic protein-2-A potential autocrine/paracrine factor in mediating the stretch activated B-type and atrial natriuretic peptide expression in cardiac myocytes[Show abstract] [Hide abstract] ABSTRACT: Haemodynamic overload exposes the heart to variety of neural, humoral and mechanical stresses. Even without the neurohumoral control of the entire organism cardiac myocytes have the ability to sense mechanical stretch and convert it into adaptive intracellular signals. This process is controlled by several growth factors. Here we show that mechanical stretch in vitro and haemodynamic overload in vivo activated the expression of bone morphogenetic protein-2 (BMP-2), while expression of BMP-4 was temporarily attenuated by stretch. BMP-2 and BMP-4 alone stimulated B-type and atrial natriuretic peptide (BNP and ANP) expression and protein synthesis, and activated transcription factor GATA-4 resembling the effects of mechanical stretch of cultured cardiac myocytes. Further, BMP antagonist Noggin was able to inhibit stretch and hypertrophic agonist induced BNP and ANP expression. Together these data provide evidence for BMP-2 as a new autocrine/paracrine factor that regulates cardiomyocyte mechanotransduction and adaptation to increased mechanical stretch.
- [Show abstract] [Hide abstract] ABSTRACT: Active involvement of extracellular matrix (ECM) and its composition regulating factors may have a central role in the pathogenesis of calcific aortic valve disease (CAVD). Thrombospondins (TSPs) are highly conserved matricellular proteins regulating inflammation, angiogenesis and ECM remodeling. These processes are strongly associated with progression of aortic valve stenosis (AS). However, the expression of TSPs in CAVD is not known. We characterized the expression of TSPs 1-4 in human aortic valves by real-time quantitative reverse transcriptase polymerase chain reaction and immunohistochemistry. Control valves (n=8), thickened and stiffened fibro(sclero)tic valves (n=8), and calcified AS valves (n=24) were compared. Furthermore, potential factors regulating TSP-2 expression was studied by western blotting and gel mobility shift assay in another set of control (n=10) and AS (n=20) valves. TSP-2 mRNA levels were increased 4.9-fold (P=0.037) and 4.8-fold (P=0.001) in fibro(sclero)tic and stenotic valves, respectively, whereas the expression of other TSPs did not change significantly. All TSPs 1-4 were detected from aortic valves by immunohistochemistry. Positive TSP-2 immunostaining was seen in the valvular myofibroblasts and patchily in endothelial cells. Semiquantitative analysis of TSP-2 staining indicated increased immunoreactivity for TSP-2 in neo vessels of fibro(sclero)tic and calcified aortic valves. Finally, when compared to controls, AS was associated with significant down regulation of Akt-pathway and diminished binding activity of nuclear factor-κB (NF-κB). We report for the first time that TSPs 1-4 are expressed in human aortic valves. CAVD is characterized by myofibroblastic proliferation and neovascularization associated upregulation of TSP-2 expression, as well as inactivation of Akt and NF-κB.
Article: Intramyocardial BNP Gene Delivery Improves Cardiac Function Through Distinct Context-Dependent Mechanisms[Show abstract] [Hide abstract] ABSTRACT: B-type natriuretic peptide (BNP) is an endogenous peptide produced under physiological and pathological conditions mainly by ventricular myocytes. It has natriuretic, diuretic, blood pressure-lowering, and antifibrotic actions that could mediate cardiorenal protection in cardiovascular diseases. In the present study, we used BNP gene transfer to examine functional and structural effects of BNP on left ventricular (LV) remodeling. Human BNP was overexpressed by using adenovirus-mediated gene delivery in normal rat hearts and in hearts during the remodeling process after infarction and in an experimental model of angiotensin II-mediated hypertension. In healthy hearts, BNP gene delivery into the anterior wall of the LV decreased myocardial fibrosis (P<0.01, n=7 to 8) and increased capillary density (P<0.05, n=7 to 8) associated with a 7.3-fold increase in LV BNP peptide levels. Overexpression of BNP improved LV fractional shortening by 22% (P<0.05, n=6 to 7) and ejection fraction by 19% (P<0.05, n=6 to 7) after infarction. The favorable effect of BNP gene delivery on cardiac function after infarction was associated with normalization of cardiac sarcoplasmic reticulum Ca(2+)-ATPase expression and phospholamban Thr17-phosphorylation. BNP gene delivery also improved fractional shortening and ejection fraction in angiotensin II-mediated hypertension as well as decreased myocardial fibrosis and LV collagen III mRNA levels but had no effect on angiogenesis or Ca(2+)-ATPase expression and phospholamban phosphorylation. Local intramyocardial BNP gene delivery improves cardiac function and attenuates adverse postinfarction and angiotensin II-induced remodeling. These results also indicate that myocardial BNP has pleiotropic, context-dependent, favorable actions on cardiac function and suggest that BNP acts locally as a key mechanical load-activated regulator of angiogenesis and fibrosis.
Article: Comparison of ear tattoo, ear notching and microtattoo in rats undergoing cardiovascular telemetry[Show abstract] [Hide abstract] ABSTRACT: Individual and permanent identification of experimental animals is a common and often essential research practice. There is little information available on the short-term effects of these procedures on the animals. In this study, seven rats were implanted with telemetric devices. The effects of three different identification methods (ear tattoo, ear notching and microtattoo) were compared. Cardiovascular data were collected for 24 h after the procedures. Time periods of 0-1, 1-4, 4-16 h (dark) and 16-24 h after the procedure were analysed separately. The most pronounced differences in measured parameters were observed during the first hour after the procedures were performed. Mean arterial pressure (MAP) was significantly higher (P < 0.012) following the ear tattoo than the microtattoo procedure by a difference of approximately 5 mmHg. Heart rate (HR) was significantly elevated (P < 0.001) after ear tattoo compared with both ear notching (Δ = 31 beats per minute [bpm]) and microtattoo (Δ = 44 bpm). During the 1-4 h period and the following dark period, the MAP was highest in the ear notching group, but no differences were observed in the HRs. During the following dark period (4-16 h) and the next day (16-24 h) differences in MAP and HR were minor. In conclusion, microtattoo appears to cause the mildest changes in HR and blood pressure. Based on these results, ear tattoo and ear notching should be replaced by microtattoo whenever possible.
Article: Parthenolide inhibits STAT3 signaling and attenuates angiotensin II-induced left ventricular hypertrophy via modulation of fibroblast activity[Show abstract] [Hide abstract] ABSTRACT: Parthenolide has shown promise in treatment of various cancers via inhibition of the transcription factor signal transducer and activator of transcription 3 (STAT3). Activation of STAT3 has been observed in left ventricular hypertrophy (LVH); however, its exact role is not known. The aim of the study was to examine the effects of parthenolide on pressure overload-induced LVH in rats. Pressure overload was induced by angiotensin II (Ang II) infusion (33 μg/kg/h) for 1 week in the presence or absence of parthenolide (0.5mg/kg/day, i.p.). Ang II infusion resulted in LVH associated with increased phosphorylation of STAT3 at Tyr705 and Ser727. Parthenolide treatment had no effect on ejection fraction, but abolished the activation of STAT3 and reduced the Ang II-induced LVH (LV posterior wall thickness in end-diastole: 2.28 ± 0.12 mm vs. 1.80 ± 0.06 mm, P<0.001). Importantly, parthenolide treatment had no effect on heart rate or blood pressure. Parthenolide treatment almost completely abolished the Ang II-induced increase in the number of cells positive for prolyl-4-hydroxylase, a marker for collagen-synthesizing cells, as well as Ang II-induced interstitial fibrosis in the left ventricles. This was associated with significant attenuation of Ang II-induced increase in mRNA levels of type 1 collagen and fibronectin. Moreover, parthenolide attenuated the Ang II-induced expression of interleukin-6, a potent pro-hypertrophic fibroblast-derived factor. We conclude that pharmacological inhibition of STAT3 signaling by parthenolide has favorable effects on pressure overload-induced LVH through attenuation of fibroblast activation. Therefore parthenolide may prove as a useful therapy for certain cardiovascular disease.
- [Show abstract] [Hide abstract] ABSTRACT: Increased production of reactive oxygen species (ROS) has been linked to the pathogenesis of contractile dysfunction in heart failure. However, it is unclear whether ROS can regulate physiological cellular processes in the myocardium. Here, we characterized the role of endogenous ROS production in the acute regulation of cardiac contractility in the intact rat heart. In isolated perfused rat hearts, endothelin-1 (ET-1, 1nmol/L) stimulated ROS formation in the left ventricle, which was prevented by the antioxidant N-acetylcysteine and the NAD(P)H oxidase inhibitor apocynin. N-acetylcysteine, the superoxide dismutase mimetic MnTMPyP, and apocynin significantly attenuated ET-1-mediated inotropic effect, which was accompanied by inhibition of extracellular signal regulated kinase 1/2 (ERK1/2) phosphorylation. Moreover, the mitochondrial K(ATP) channel blocker 5-HD, and the mitochondrial large conductance calcium activated potassium channel blocker paxilline, but not the sarcolemmal K(ATP) channel blocker HMR 1098 attenuated the inotropic response to ET-1. However, ET-1-induced ROS generation was not abolished by inhibiting mitochondrial K(ATP) channel opening. In contrast to ET-1 stimulation, the positive inotropic effect of β(1)-adrenergic receptor agonist dobutamine (250nmol/L) was significantly augmented by N-acetylcysteine and apocynin. Moreover, dobutamine-induced phospholamban phosphorylation was markedly enhanced by apocynin. In conclusion, NAD(P)H oxidase-derived ROS play a physiological role in the acute regulation of cardiac contractility in the intact rat heart. Our results reveal that ET-1-induced increase in cardiac contractility is partially dependent on enhanced ROS generation, which in turn, activates the ERK1/2 pathway. On the other hand, β-adrenergic receptor-induced positive inotropic effect and phospholamban phosphorylation is enhanced by NAD(P)H oxidase inhibition.
Article: Collagen XV Is Necessary for Modeling of the Extracellular Matrix and Its Deficiency Predisposes to Cardiomyopathy[Show abstract] [Hide abstract] ABSTRACT: The extracellular matrix (ECM) is a major determinant of the structural integrity and functional properties of the myocardium in common pathological conditions, and changes in vasculature contribute to cardiac dysfunction. Collagen (Col) XV is preferentially expressed in the ECM of cardiac muscle and microvessels. We aimed to characterize the ECM, cardiovascular function and responses to elevated cardiovascular load in mice lacking Col XV (Col15a1(-/-)) to define its functional role in the vasculature and in age- and hypertension-associated myocardial remodeling. Cardiac structure and vasculature were analyzed by light and electron microscopy. Cardiac function, intraarterial blood pressure, microhemodynamics, and gene expression profiles were studied using echocardiography, telemetry, intravital microscopy, and PCR, respectively. Experimental hypertension was induced with angiotensin II or with a nitric oxide synthesis inhibitor. Under basal conditions, lack of Col XV resulted in increased permeability and impaired microvascular hemodynamics, distinct early-onset and age-dependent defects in heart structure and function, a poorly organized fibrillar collagen matrix with marked interstitial deposition of nonfibrillar protein aggregates, increased tissue stiffness, and irregularly organized cardiomyocytes. In response to experimental hypertension, Col15a1 gene expression was increased in the left ventricle of wild-type mice, and mRNA expression of natriuretic peptides (ANP and BNP) and ECM modeling were abnormal in Col15a1(-/-) mice. Col XV is necessary for ECM organization in the heart, and for the structure and functions of microvessels. Col XV deficiency leads to a complex cardiac phenotype and predisposes the subject to pathological responses under cardiac stress.
Article: Mitochondrial uncoupling downregulates calsequestrin expression and reduces SR Ca stores in cardiomyocytes[Show abstract] [Hide abstract] ABSTRACT: Mitochondrial cardiomyopathy is associated with deleterious remodelling of cardiomyocyte Ca(2+) signalling that is partly due to the suppressed expression of the sarcoplasmic reticulum (SR) Ca(2+) buffer calsequestrin (CASQ2). This study was aimed at determining whether CASQ2 downregulation is directly caused by impaired mitochondrial function. Mitochondrial stress was induced in cultured neonatal rat cardiomyocytes by means of the mitochondrial uncoupler carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP). Ca(2+) transients and reactive oxygen species (ROS) were measured by confocal microscopy using the indicators fluo-4 and MitoSOX red, respectively. Mitochondrial stress led to concentration-dependent downregulation of calsequestrin (CASQ2) and changes in the Ca(2+) signals of the cardiomyocytes that were accompanied by reduction in SR Ca(2+) content and amplitude and duration of Ca(2+) sparks. Caspase 3, p38, and p53 inhibitors had no effect on FCCP-induced CASQ2 downregulation; however, it was attenuated by the ROS scavenger N-acetylcysteine (NAC). Importantly, NAC not only decreased FCCP-induced ROS production, but it also restored the Ca(2+) signals, SR Ca(2+) content, and Ca(2+) spark properties to control levels. Mitochondrial uncoupling results in fast transcriptional changes in CASQ2 expression that manifest as compromised Ca(2+) signalling, and these changes can be prevented by ROS scavengers. As impaired mitochondrial function has been implicated in several cardiac pathologies as well as in normal ageing, the mechanisms described here might be involved in a wide spectrum of cardiac conditions.
Article: Mitochondrial uncoupling downregulates calsequestrin expression and reduces SR Ca2+ stores in cardiomyocytes[Show abstract] [Hide abstract] ABSTRACT: Aims: Mitochondrial cardiomyopathy is associated with deleterious remodelling of cardiomyocyte Ca²⁺ signalling that is partly due to the suppressed expression of the sarcoplasmic reticulum (SR) Ca²⁺ buffer calsequestrin (CASQ2). This study was aimed at determining whether CASQ2 downregulation is directly caused by impaired mitochondrial function. Methods and results: Mitochondrial stress was induced in cultured neonatal rat cardiomyocytes by means of the mitochondrial uncoupler carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP). Ca²⁺ transients and reactive oxygen species (ROS) were measured by confocal microscopy using the indicators fluo-4 and MitoSOX red, respectively. Mitochondrial stress led to concentration-dependent downregulation of calsequestrin (CASQ2) and changes in the Ca²⁺ signals of the cardiomyocytes that were accompanied by reduction in SR Ca²⁺ content and amplitude and duration of Ca²⁺ sparks. Caspase 3, p38, and p53 inhibitors had no effect on FCCP-induced CASQ2 downregulation; however, it was attenuated by the ROS scavenger N-acetylcysteine (NAC). Importantly, NAC not only decreased FCCP-induced ROS production, but it also restored the Ca²⁺ signals, SR Ca²⁺ content, and Ca²⁺ spark properties to control levels. Conclusion: Mitochondrial uncoupling results in fast transcriptional changes in CASQ2 expression that manifest as compromised Ca²⁺ signalling, and these changes can be prevented by ROS scavengers. As impaired mitochondrial function has been implicated in several cardiac pathologies as well as in normal ageing, the mechanisms described here might be involved in a wide spectrum of cardiac conditions. © The Author 2010. Published on behalf of the European Society of Cardiology. All rights reserved.
Article: Tumour necrosis factor-like weak inducer of apoptosis (TWEAK) and its receptor Fn14 during cardiac remodelling in rats[Show abstract] [Hide abstract] ABSTRACT: Accumulating evidence supports the concept that proinflammatory cytokines play an essential role in the failing heart. We examined the concomitant tumour necrosis factor-like weak inducer of apoptosis (TWEAK)/Fn14 expression in myocytes in vitro as well as in vivo in cardiac remodelling. We assessed TWEAK and its receptor Fn14 expression in response to angiotensin (Ang) II, myocardial infarction (MI) as well as to local adenovirus-mediated p38 gene transfer in vivo. The effect of various hypertrophic factors and mechanical stretch was studied in neonatal rat ventricular myocyte cell culture. Ang II increased Fn14 levels from 6 h to 2 weeks, the greatest increase in mRNA levels being observed at 6 h (6.3-fold, P < 0.001) and protein levels at 12 h (4.9-fold, P < 0.01). TWEAK mRNA and protein levels remained almost unchanged during Ang II infusion. Likewise, a rapid and sustained elevation of Fn14 mRNA and protein levels in the left ventricle was observed after experimental MI. Moreover, local p38 gene transfer increased Fn14 mRNA and protein but not TWEAK levels. Fn14 immunoreactive cells were mainly proliferating non-myocytes in the inflammation area while TWEAK immunoreactivity localized to cardiomyocytes and endothelial cells of the coronary arteries. Hypertrophic agonists and lipopolysaccharide increased Fn14 but not TWEAK gene expression in neonatal rat myocytes, while mechanical stretch upregulated Fn14 and downregulated TWEAK gene expression. In conclusion, the cardiac TWEAK/Fn14 pathway is modified in response to myocardial injury, inflammation and pressure overload. Furthermore, our findings underscore the importance of Fn14 as a mediator of TWEAK/Fn14 signalling in the heart and a potential target for therapeutic interventions.
- [Show abstract] [Hide abstract] ABSTRACT: Recent data suggest that GATA-4 is an antiapoptotic factor required for adaptive responses and a key regulator of hypertrophy and hypertrophy-associated genes in the heart. As a leading cause of chronic heart failure, reversal of postinfarction left ventricular remodeling represents an important target for therapeutic interventions. Here, we studied the role of GATA-4 as a mediator of postinfarction remodeling in rats. Myocardial infarction, caused by ligating the left anterior descending coronary artery, significantly decreased the DNA binding activity of GATA-4 at day 1, whereas at 2 weeks the GATA-4 DNA binding was significantly upregulated. To determine the functional role of GATA-4, peri-infarct intramyocardial delivery of adenoviral vector expressing GATA-4 was done before left anterior descending coronary artery ligation. Hearts treated with GATA-4 gene transfer exhibited significantly increased ejection fraction and fractional shortening. Accordingly, infarct size was significantly reduced. To determine the cardioprotective mechanisms of GATA-4, myocardial angiogenesis, rate of apoptosis, c-kit+ cardiac stemlike cells, and genes regulated by GATA-4 were studied. The number of capillaries and stemlike cells was significantly increased, and decreased apoptosis was observed. These results indicate that the reversal of reduced GATA-4 activity prevents adverse postinfarction remodeling through myocardial angiogenesis, antiapoptosis, and stem cell recruitment. GATA-4-based gene transfer may represent a novel, efficient therapeutic approach for heart failure.
Article: Metoprolol Treatment Lowers Thrombospondin-4 Expression in Rats with Myocardial Infarction and Left Ventricular Hypertrophy[Show abstract] [Hide abstract] ABSTRACT: Thrombospondins are matrix proteins linked to extracellular matrix remodelling but their precise role in the heart is not known. In this study, we characterised left ventricular thrombospondin-1 and -4 expression in rats treated with a beta-blocker metoprolol during the remodelling process in response to pressure overload and acute myocardial infarction. Left ventricular thrombospondin-1 and thrombospondin-4 mRNA levels increased 8.4-fold (p < 0.001) and 7.3-fold (p < 0.001) post-infarction, respectively. Metoprolol infusion by osmotic minipumps (1.5 mg/kg/hr) for 2 weeks after myocardial infarction decreased thrombospondin-1 and thrombospondin-4 mRNA levels (55% and 50%, respectively), improved left ventricular function, and attenuated left ventricular remodelling with reduction of left ventricular atrial natriuretic peptide and brain natriuretic peptide gene expression. Thrombospondin-1 and -4 mRNA levels correlated positively with echocardiographic parameters of left ventricular remodelling as well as with atrial natriuretic peptide and brain natriuretic peptide gene expression. Moreover, there was a negative correlation between left ventricular ejection fraction and thrombospondin-1 mRNA levels. In 12-month-old spontaneously hypertensive rats with left ventricular hypertrophy, metoprolol decreased left ventricular thrombospondin-4 levels and attenuated remodelling while thrombospondin-1, atrial natriuretic peptide and brain natriuretic peptide mRNA levels as well as left ventricular function remained unchanged. In metoprolol-treated spontaneously hypertensive rats, thrombospondin-4 gene expression correlated with parameters of left ventricular remodelling, while no correlations between thrombospondins and natriuretic peptides were observed. These results indicate that thrombospondin-1 expression is linked exclusively to left ventricular remodelling process post-infarction while thrombospondin-4 associates with myocardial remodelling both after myocardial infarction and in hypertensive heart disease suggesting that thrombospondins may have unique roles in extracellular matrix remodelling process.
- [Show abstract] [Hide abstract] ABSTRACT: According to the European recommendations rodents should be provided with a nest box if there is insufficient nesting material to build a complete, covered nest. Rats are generally poor nest builders; hence an additional structure is needed. Optimally, housing refinement may be combined with better science; at least it should not detract from the scientific integrity. In order to evaluate these options, there is a need to assess the items used in individual research projects. Studies investigating molecular mechanisms of cardiac hypertrophy and heart failure are typically long-lasting studies; therefore, refinement of the housing of rats in these studies is important. The aim of this study was to evaluate in rats whether a wooden tube has any impact on cardiac morphology or on basal gene expression of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP); known markers of cardiac overload, hypertrophy and heart failure. The experimental protocol simulated cardiovascular studies, but without any surgical operations. A total of 42 male Hsd:SD rats were used in an eight-week experiment. After weaning, the experimental group was provided with a rectangular aspen tube and nesting material, and the control group with only nesting material. ANP and BNP gene expression were measured from the left ventricles with Northern blot analysis postmortem along with the absolute weights of the whole heart, left and right atria and left and right chambers. The weights of the whole heart and left chamber were also analysed in relation to body weight. No statistically significant differences were observed in any of these variables. The inter-individual variation was also unchanged by the cage item. In conclusion, the aspen tube does not disrupt research results or alter the number of animals needed and can therefore be recommended for enrichment purposes in cardiovascular studies.
- [Show abstract] [Hide abstract] ABSTRACT: Dyxin is a novel LIM domain protein acting as a transcriptional cofactor with GATA transcription factors. Here, we characterized dyxin as a p38 mitogen-activated protein kinase (MAPK) regulated gene, since combined upstream MAPK kinase 3b and wild-type p38 alpha MAPK gene transfer increased left ventricular dyxin mRNA and protein levels in vivo. We also studied cardiac dyxin expression in experimental models of pressure overload and myocardial infarction (MI) in vivo. Angiotensin II infusion increased left ventricular dyxin mRNA levels (9.4-fold, p<0.001) rapidly at 6 h followed by induction of protein levels. Furthermore, simultaneous administration of p38 MAPK inhibitor SB203580 abolished angiotensin II-induced activation of dyxin gene expression. During the post-infarction remodeling process, increased dyxin mRNA levels (7.7-fold, p<0.01) were noted at day 1 followed by the increase in proteins levels at 2 weeks after MI (1.5-fold, p<0.05). Moreover, direct wall stretch by using isolated rat heart preparation as well as direct mechanical stretch of cardiomyocytes in vitro activated dyxin gene expression within 1 h. Our results indicate that dyxin expression is rapidly upregulated in response to mechanical load, this increase being at least partly mediated by p38 MAPK. These results suggest that dyxin may play an important role in regulating hypertrophic process.
Article: Hypoxia-Induced Expression of Bradykinin Type-2 Receptors in Endothelial Cells Triggers NO Production, Cell Migration, and Angiogenesis[Show abstract] [Hide abstract] ABSTRACT: Bradykinin receptors are differentially expressed in the coronary vascular endothelium of rat and human hearts during the pathogenesis of heart failure, but the mechanisms responsible for this regulation have remained vague. Here we show by quantitative real-time PCR, Western blot analysis, and immunohistochemistry, that hypoxia triggers the expression of bradykinin type-2 receptors (BK-2Rs) in cultured human coronary artery endothelial cells (HCAECs), in isolated rat cardiac microvascular endothelial cells (RCMECs), and in rat hearts subjected to ligation of the left anterior descending coronary artery. Mild hypoxia (5% O(2)) induced a fourfold temporal increase in BK-2R mRNA expression in HCAECs, which was also observed at the protein level, whereas severe hypoxia (1% O(2)) slightly inhibited the mRNA expression of BK-2Rs. In addition, HOE-140, a BK-2R antagonist, inhibited mRNA and protein expression of BK-2Rs. The BK-2Rs induced by mild hypoxia were biologically active, that is, capable of inducing intracellular production of nitric oxide (NO) upon activation of HCAECs with bradykinin (BK), a response attenuated by HOE-140. In rat hearts recovering from myocardial infarction, BK-2Rs were upregulated in the endothelium of vessels forming at the border zone between fibrotic scar tissue and healthy myocardium. Furthermore, in an in vitro wound-healing assay, RCMEC migration was increased under mild hypoxic culture conditions in the presence of BK and was attenuated with HOE-140. Our present results show that mild hypoxia triggers a temporal expression of functional BK-2Rs in human and rat endothelial cells and support a role for BK-2Rs in hypoxia-induced angiogenesis.
Article: Oral levosimendan prevents postinfarct heart failure and cardiac remodeling in diabetic Goto-Kakizaki rats[Show abstract] [Hide abstract] ABSTRACT: Diabetes increases the risk for fatal myocardial infarction and development of heart failure. Levosimendan, an inodilator acting both via calcium sensitization and opening of ATP-dependent potassium channels, is used intravenously for acute decompensated heart failure. The long-term effects of oral levosimendan on postinfarct heart failure are largely unknown. To examine whether oral treatment with levosimendan could improve cardiac functions and prevent cardiac remodeling after myocardial infarction in a rodent model of type 2 diabetes, the Goto-Kakizaki rat. Myocardial infarction (MI) was induced to diabetic Goto-Kakizaki and nondiabetic Wistar rats by coronary ligation. Twenty-four hours after surgery, Goto-Kakizaki and Wistar rats were randomized into four groups: MI group without treatment, MI group with levosimendan for 12 weeks (1 mg/kg per day), sham-operated group, sham-operated group with levosimendan. Blood pressure, cardiac functions as wells as markers of cardiac remodeling were determined. In Goto-Kakizaki rats, MI induced systolic heart failure, pronounced cardiac hypertrophy in the remote area, and sustained cardiomyocyte apoptosis. Postinfarct cardiac remodeling was associated with increased atrial natriuretic peptide, interleukin-6 and connective tissue growth factor mRNA expressions, as well as three-fold increased cardiomyocyte senescence, measured as cardiac p16 mRNA expression. Levosimendan improved cardiac function and prevented postinfarct cardiomyocyte hypertrophy, cardiomyocyte apoptosis, and cellular senescence. Levosimendan also ameliorated MI-induced atrial natriuretic peptide, IL-6, and connective tissue growth factor overexpression as well as MI-induced disturbances in calcium-handling proteins (SERCA2, Na-Ca exchanger) without changes in diabetic status or systemic blood pressure. In nondiabetic Wistar rats, MI induced systolic heart failure; however, the postinfarct cardiac remodeling was associated with less pronounced cardiac hypertrophy, cardiomyocyte apoptosis, inflammatory reaction, and induction of cellular senescence. Levosimendan only partially prevented postinfarct heart failure and cardiac remodeling in Wistar rats. Our findings suggest a therapeutic role for oral levosimendan in prevention of postinfarct heart failure and cardiac remodeling in type 2 diabetes and underscore the importance of sustained cardiomyocyte apoptosis and induction of cellular senescence in the pathogenesis.
- [Show abstract] [Hide abstract] ABSTRACT: Matrix Gla protein (MGP) expression is increased in cardiac hypertrophy, but the precise mechanisms regulating its expression are unknown. Here we characterized the effect of pressure overload and myocardial infarction in vivo as well as mechanical stretch and hypertrophic agonists in vitro on MGP expression. When angiotensin II (Ang II) was administered by osmotic minipumps, left ventricular (LV) MGP mRNA levels increased significantly from 6 h to 2 weeks, whereas intravenous arginine(8)-vasopressin increased LV MGP mRNA levels within 4 h. During post-infarction remodeling process, MGP mRNA levels were elevated at 24 h (1.3-fold, p<0.05) and the maximal increase was observed at 4 weeks (2.8-fold, p<0.01). Ang II increased MGP mRNA levels 20% (p<0.05) in neonatal rat cardiac myocytes and 40% (p<0.05) in cardiac fibroblasts, whereas endothelin-1 decreased MGP mRNA levels 30% (p<0.01) in myocytes and had no effect in fibroblasts. Cyclic mechanical stretch resulted in reduction of MGP gene expression in both cardiac myocytes and fibroblasts. These results demonstrate that MGP is rapidly upregulated in response to cardiac overload well before the development of LV hypertrophy and post-infarction remodeling process. Our results also suggest that Ang II may be involved in mediating load-induced activation of MGP expression.
Article: Effects of calcium sensitizer OR-1986 on cardiovascular mortality and myocardial remodelling in hypertensive Dahl/Rapp rats[Show abstract] [Hide abstract] ABSTRACT: Calcium-sensitizing agents have been shown to improve cardiac function in patients suffering from acute decompensated heart failure, however, their long-term effects on cardiac remodeling and cardiovascular mortality are still largely unknown. In the present study we tested the hypothesis whether OR-1896, an active and long-lasting metabolite of calcium sensitizer levosimendan, prevents cardiovascular mortality and hypertension-induced myocardial remodelling in salt-sensitive Dahl/Rapp rats. OR-1896 was given orally to Dahl/Rapp SS rats on high-salt diet (NaCl 7% w/w) for 7 weeks at two different doses (0.5 and 0.05 mg/kg). OR-1896 prevented salt-induced cardiovascular mortality (survival rate 75 % in OR-1896 treated groups vs 38 % in untreated controls, p<0.01), ameliorated cardiac hypertrophy and improved systolic functions of the heart without major influence on systemic blood pressure. OR-1896 also ameliorated salt-induced increase in cardiac ANP mRNA expression and plasma BNP level. Salt-induced cardiac remodelling was associated with 4-fold increase in cardiac p16(INK4a) mRNA expression, a marker of cellular senescence. OR-1896 dose-dependently ameliorated cardiomyocyte senescence. Our findings suggest a therapeutic role for OR-1896 in the prevention of cardiac remodelling in salt-sensitive forms of hypertension. The present study also underscores the importance of cellular senescence in the pathogenesis of salt-induced hypertensive heart disease.
Dataset: Table S1[Show abstract] [Hide abstract] ABSTRACT: The rates of state 3 and 4 respiration of liver and heart mitochondria with various substrates. The liver and heart mitochondria were isolated as described [1] after 12 h fast. The incubations were carried out with 0.5–1.2 mg/ml of mitochondrial protein at 30°C in the appropriate medium (Materials and Methods) and 2 mM glutamate, 2 mM malate, 2 mM pyruvate, or 2 mM succinate. ADP was used at 1 mM concentration. The rate of oxygen consumption (nmol O2/min per mg protein) was monitored with Clark type oxgen electrode. The results are expressed as mean±standard deviation. The statistical significance was estimated using the Student's t test and the P-values are given. (0.02 MB DOC)
Top Journals
- Circulation Research (4)
- Circulation (3)
- Circulation (3)
- Hypertension (3)
- Circulation Research (3)
Oulu University HospitalUleoborg, Oulu, Finland
University of Oulu
Uleoborg, Northern Ostrobothnia, Finland
- Department of Pharmacology and Toxicology
Mayo Clinic - Rochester
Рочестер, Minnesota, United States
- Department of Cardiovascular Diseases
Mayo Foundation for Medical Education and Research
Рочестер, Michigan, United States
- Division of Cardiovascular Diseases


text:
I'm not getting an answer on this at the support forum from the theme, so I move to here...
Having trobles with my actual theme that my preview of the gallery is not the width the theme is.
I chose the layout with no sidebars and set it to max width of 1.040px.
It works well with my photoblog.
The landing page (the blog) looks perfect (for me) if I post a picture (format: picture) - it fits...!
But if I take the format "gallery" and the theme builds the preview of the first three pics, then I have a gap on the right side as you can see on my site right now (http://www.geri.at).
How to avoid that...?
At least, is there a chance to center it?
Does anybody know how to change the height of the nav bar?
I found some code snippes on the web,
but they are not working for some reason…
PS: If somebody knows a good alternative to the pinboard theme, I really would appreciate it.
Important for me is a very small header. The photo/gallery should be the most important thing on my site...

subset: commoncrawl


text:
Two Greencastle area men accused of selling large quantities of heroin waived their right to preliminary hearings Tuesday in Franklin County Court.
Jack Silvers Jr., 43, and James Baker, 31, both of 14884 Sherwood Drive in Antrim Township, were arrested Jan. 29 after members of the Franklin County Drug Task Force searched their home.
During the search, investigators found heroin, marijuana, two handguns — one of which had been reported stolen from Hagerstown, items used for weighing and packaging the drugs and $3,000 cash, according to a news release from the task force.
Local residents and informants tipped off police to Silvers and Baker, who police say acquired heroin from Maryland and brought it Pennsylvania where they distributed from their home and parking lots of local businesses.
The two are being held in Franklin County Jail in lieu of $75,000 bond each.
No date has been set for their trials.
Location, ST | website.com


text:
RATINGS: 250 WEIGHTED AVG: 2.85/5 SEASONAL: Special EST. CALORIES: 225 ABV: 7.5%
A fury of dots and dashes come trough the receiver. The translator turns in amazement. "A Zero IBU IPA by two breweries separated by sea?" His commanding officer lays a grizzled hand on his shoulder as his eyes narrow toward the horizon. "No bastard ever won a war by playing it safe, son."
The International Arms Race is a new type of battle collaboration between hardcore American craft brewery Flying Dog and BrewDog.
Zero IBU IPA is an ale brewed with berries and herbs.
2 IPAs that rock.
Hunt them both down and see which beer you think deserves to win the battle and the war.
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Focus Stocks
Maxwell Technologies to Report First Quarter Financial Results on April 26; Annual Stockholders Meeting Set for May 9
Published on Thursday, 19 April 2012 07:21 Written by TradersHuddle Staff
SAN DIEGO, April 19, 2012 /PRNewswire/ -- Maxwell Technologies, Inc. (Nasdaq: MXWL) will issue its financial results news release for the first quarter ended March 31, 2012, at 4 p.m. (EDT) on April 26, 2012. Management will conduct a conference call and simultaneous webcast to discuss first quarter results and the future outlook one hour later, at 5 p.m. (EDT).
The news release will be distributed by wire and posted simultaneously on the company's website, http://investors.maxwell.com, at 4 p.m. (EDT) on April 26. Anyone wishing to be added to the company's email distribution list for news releases may do so by calling (858) 503-3434.
The public is invited to listen to the conference call by dialing, toll-free (800) 862-9098 from the U.S. and Canada, or (785) 424-1051 for international callers, and entering the conference ID, MAXWELL. The conference call, webcast and subsequent archived replay may be accessed via the following link: http://investors.maxwell.com.
The company's Annual Meeting of Stockholders will be conducted at 11 a.m. (PDT), May 9, 2012, at the Courtyard by Marriott Hotel, 8651 Spectrum Center Boulevard in San Diego.
Maxwell is a leading developer and manufacturer of innovative, cost-effective energy storage and power delivery solutions. Our ultracapacitor products provide safe and reliable power solutions for applications in consumer and industrial electronics, transportation and telecommunications. Our high-voltage grading and coupling capacitors help to ensure the safety and reliability of electric utility infrastructure and other applications involving transport, distribution and measurement of high-voltage electrical energy. Our radiation-mitigated microelectronic products include power modules, memory modules and single board computers that incorporate powerful commercial silicon for superior performance and high reliability in aerospace applications. For more information, please visit our website: www.maxwell.com.
SOURCE Maxwell Technologies, Inc.
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Plug-ins: Comment link manager
When I log in, I notice I have a bunch of comments waiting for moderation. Clicking on them shows it's a bunch of spam. Under discussion, I have, "users must be registered and logged in to comment." selected. If you click to add a comment, it takes you to the login page. The problem is these comments keep showing up waiting to be approved even with no registered users. I'm migrating a blog to this wordpress install and in the process of rebuilding all the older posts, and I'd like to know if I should just stop now and go back to the other software, or am I missing something obvious. Any clues on where to look?


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Tuesday Nov. 18, 2008
MADRID, Spain (AP) -- Seve Ballesteros has been released from intensive care following brain surgery on a malignant tumor.
Madrid's La Paz hospital said Tuesday the 51-year-old golf great would remain in the hospital for rehab. Ballesteros had three operations in 18 days. There was no timetable for his release. Ballesteros was admitted to the hospital Oct. 6 after fainting at Madrid's international airport. He underwent a 6 1/2-hour operation Oct. 24 to remove the tumor and reduce swelling around the brain.
(Copyright 2008 by The Associated Press. All Rights Reserved.)


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Tourist is victim of latest gang-rape in IndiaThe Associated Press | March 17,2013NEW DELHI A Swiss woman who was on a cycling trip in central India with her husband has been gang raped by eight men, police said Saturday. The attack comes three months after the fatal gang rape of a woman aboard a New Delhi bus outraged Indians.
Authorities detained and questioned 13 men in connection with the latest attack, which occurred Friday night as the couple camped out in a forest in Madhya Pradesh state after bicycling from the temple town of Orchha, local police officer R.K. Gurjar said.
The men beat the couple and gang raped the woman, he said. They also stole the couples mobile phone, a laptop computer and 10,000 rupees ($185).
The woman, 39, was treated at a hospital in the nearby city of Gwalior, Gurjar said, adding that she and her husband apparently suffered no major injuries.
A photo showed the woman her identity concealed with a hood walking while being escorted by police to the hospital. Indian law does not allow rape victims to be identified publicly to protect victims from the stigma that is attached to rape in the conservative country.
Police detained 13 men and questioned them, Gurjar said. Six of the men were released after questioning. No other details were immediately available.
Indian television stations showed scores of police searching the forest where the attack occurred.
Swiss Foreign Ministry spokesman Tilman Renz described the case as deeply disturbing and said Swiss diplomats were assisting the couple.
The diplomats called on Indian authorities to do everything to quickly find the perpetrators so that they can be held accountable, Renz said in a statement.
Last month, the Swiss government issued a travel notice for India that included a warning about increasing numbers of rapes and other sexual offenses in the South Asian nation.
India has seen outrage and widespread protests against attacks on women since Decembers fatal gang-rape of a young woman on a moving bus in New Delhi, the capital. The crime horrified Indians and set off nationwide protests about Indias treatment of women and spurred the government to hurry through a new package of laws to protect them.
One of six suspects in the December attack was found dead in a New Delhi jail this past week. Authorities said he hanged himself, but his family and lawyer insisted foul play was involved. A magistrate is investigating. Four other men and a juvenile remain on trial for the attack.MORE IN This Just InBENNINGTON A Sandgate man with a previous conviction for a sex crime against a child in New... Full StoryThousands of Vermonters will have the chance to get their drivers licenses back starting Thursday. Full StoryA Castleton man, who police said has had several incidents at Fowler Services, denied striking... Full Story
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Special offer: now 10% off original price of $3,300
Fermented Artisanal Foods: Culinary Trend Tracking Series.
Fermentation is one of the hottest trends in culinary circles today. Coupled with an ongoing consumer interest in both artisan foods and food functionality, fermented foods that provide health benefits are appearing on restaurant menus, in farmers' markets and high-end grocers across the country. This trend, Packaged Facts believes, will continue to grow and extend beyond the traditional enclaves of the foodies.
Fermented Artisanal Foods: Culinary Trend Tracking Series, the latest in Packaged Facts' Culinary Trend Tracking Series, explores seven different food and beverages that encompass thematic avenues of opportunity for food businesses. Fermented Artisanal Foods: Culinary Trend Tracking Series charts how current lifestyle and demographic shifts open fresh menu and packaged food opportunities related foods fermented with care, which extends the potential for innovation deeper into meal, snack and beverage territory.
Fermented foods are finding artisan, regional and chef-driven takes. Their authenticity is rooted in hundreds of years of craft preparation and artisan pride. Regional preparations of fermented foods like craft pickles and small-batch whiskey are being elevated in the preferences of consumers looking for artisan foods. In addition to growing artisan appreciation of these foods, more consumers are preferring foods that contain ingredients geared toward wellness. The probiotic goodness of some of these foods, such as kimchi, lactic-acid fermented craft pickles and miso are appealing to those looking to boost digestive health. Propelled by culinary interest in umami flavor, also known as the fifth taste, fish sauce and dried shrimp are seeing larger roles as ingredients in the kitchens of popular restaurants and foodservice operations as they carve their path toward consumer homes.
The profiles in the culinary report demonstrate the opportunity-scape of the popularity of fermentation:
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Hey Guys,
I'm using the latest version of Word Press and Feed WP. It seems that some of the feeds are showing blank pages. However, I have read to disable optimize and certain stuff like that. I've tried that and it still doesn't work. The only option I'm thinking is to delete it. If anybody has a fix for this, please let me know.


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I'm working my way through updating (http://codex.wordpress.org/Upgrading_WordPress)
Why is backing up so complicated? There is the 5 minute installation that is easy, but the backup process has taken me more than half hour to follow the instructions.
1) Seems like someone should have written a plugin to do it automatically - have they? I have blogs on three other sites besides the one I'm working on at present and am tempted to just not bother updating, though I know that can be dangerous.
2) I used my ftp to put everything on my hard drive, but my next step is to unzip the upgrade so that I can ftp it back to server. Will that overwrite anything critical that I should save in a different location first?


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Do You Have a Video Game Curfew In Your House?
Set time aside for tech-free nights. With many family oriented games out right now, it is easy to get caught up playing games along side your kids. Try focusing on setting time aside to play with your kids while not staring at a screen. Take out those dusty old board games, maybe you can impress your kids on how much fun they can have without controlling characters through a joystick.
Lead by example. Adults can get into games just as much as teenagers do. It is important stick to your own rules, what you do directly affects how your kids perceive things. If they are watching you play video games during "off limit" hours, they will believe it's okay to break the rules.
Limit their time. Kids (and adults) love to fudge on how much time they've been enjoying while playing games. Keep the arguments to a limit by setting an exact time limit with something like the GameDR Universal Video Game Timer. It could be the best $11 you've ever spent if you've got someone who likes to fudge/fib how long they've been playing.
Video game addiction is a very real thing, and the associated risk with inactivity could be deadly. What are some of your tips for keeping your love for gaming in check? Let us know in the comments below!
(Image: hyku licensed for use under Creative Commons)


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This video, which counters a recent TV ad sponsored by American Crossroads, a Karl Rove outfit, was produced by a liberal organization calling itself the Bridge Project.
Click here to view the embedded video.
Read more
addressLocation, ST | website.com
McPhersonSentinel - McPherson, KS | mcphersonsentinel.com
Your privacy is important, read our privacy policy.


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Taj Mahal
Dallas, TX 75231
- Mon - Thu 11:00 AM - 2:30 PM 5:30 PM - 10:00 PM Fri 11:00 AM - 2:30 PM 5:30 PM - 10:30 PM Sat 11:30 AM - 2:30 PM 5:30 PM - 10:30 PM Sun 11:30 AM - 2:30 PM 5:00 PM - 9:00 PM
- $$
- Catering, Delivery, Family Style, Order Online, Private Party, Smoking, Takeout
- Dinner, Lunch
- Parking Lot Available
- Reservations Accepted, Reservations Recommended
Get the Things to Do Newsletter
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Taj Mahal
Thursday, November 2, 2006 at 3 a.m. by Darryl Smyers
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Temple fare
Thursday, March 21, 1996 at 3 a.m. by Mary Brown Malouf
Indian food in Dallas has almost gotten to the level of Mexican food:You can expect to find a usable Indian restaurant within reasonable driving distance from anywhere in the area. The name Taj Mahal inspires lofty expectations, eve...
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The Wimbledon champion started out well as he took the opening set and led 3-0 in the second, but struggled thereafter against the world number 40 as he lost 3-6 6-4 6-2.
World number four Murray struggled on serve throughout, however, getting less than half his first serves in during the deciding set - while the 30-year-old Mayer was almost untouchable, losing just three points on his own serve in the closing set.
The manner of the defeat was the big worry for Murray's fans: in what was only his ATP Tour second match since back surgery last September, the way his game crumbled prompted fears that he may not yet be fully recovered.
Any such worries appeared to have been allayed by his dominant win in the opening round - a 6-0 6-0 victory over Mousa Zayed - but the defeat against the wily German veteran spells major trouble for Murray's hopes at the Australian Open, where he has made the final in three of the last four years.
The Scot will head to Melbourne - where the first Grand Slam of the year begins on January 13 - having played just two ATP Tour matches, in addition to two exhibition matches.
"To be honest, in my mind, the match was finished at 3-6, 0-3," Mayer told the ATP website. "[But] I tried to play more aggressively, tried to risk something more. Of course for sure he didn't play his best tennis.
"I knew before the match if I would have a chance, it's probably today. He didn't play for a long time. [So] I took my chance."
Top seed Rafael Nadal nearly joined Murray out of the tournament but he ground out a tough 6-3 6-7(3) 6-3 victory over Germany's Tobias Kamke.
Second seed David Ferrer was beaten though as he was upset 6-4 7-5 by Daniel Brands.
Murray and Ferrer were not the only seeds to fall in Qatar either: eighth seed Fernando Verdasco also went out, losing 4-6 7-6(0) 6-2 against Romania's Victor Hanescu while sixth seed Philipp Kohlschreiber lost 7-6(4) 7-6(7) to Peter Gojowczyk in an all-German affair.
Results from the Qatar Open Men's Singles Round 2 matches on Wednesday
Daniel Brands (Germany) beat 2-David Ferrer (Spain) 6-4 7-5
1-Rafa Nadal (Spain) beat Tobias Kamke (Germany) 6-3 6-7(3) 6-3
7-Ernests Gulbis (Latvia) beat Lukasz Kubot (Poland) 6-2 4-6 6-3
Dustin Brown (Germany) beat Ivo Karlovic (Croatia) 3-6 7-6(13) 6-4
Victor Hanescu (Romania) beat 8-Fernando Verdasco (Spain) 4-6 7-6(0) 6-2
Florian Mayer (Germany) beat 3-Andy Murray (Britain) 3-6 6-4 6-2
Peter Gojowczyk (Germany) beat 6-Philipp Kohlschreiber (Germany) 7-6(4) 7-6(7)
Results from the Chennai Open Men's Singles Round 2 matches on Wednesday
7-Edouard Roger-Vasselin (France) beat Jiri Vesely (Czech Republic) 6-4 6-4
Dudi Sela (Israel) beat 2-Mikhail Youzhny (Russia) 3-1
Results from the Brisbane International Men's Singles Round 2 matches on Wednesday
1-Roger Federer (Switzerland) beat Jarkko Nieminen (Finland) 6-4 6-2
2-Kei Nishikori (Japan) beat Matthew Ebden (Australia) 6-2 6-4
Marin Cilic (Croatia) beat 5-Grigor Dimitrov (Bulgaria) 7-5 7-5
Marinko Matosevic (Australia) beat Sam Querrey (U.S.) 5-7 7-6(3) 6-4
- Tennis
- Sports & Recreation
- ATP Tour


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Shy but opinionated, quiet yet strong, openminded but know what I like and want. Passionate, strong-willed, stubborn. Value honesty, friendship, intelligence, humor and integrity highly.I have a submissive side. Please show respect and understand that just because I am a submissive with a little side, I will not be pushed around. I am friendly, but absolutely not interested in random cybering. If I don't know you, I will not talk in private with you (including whispers) or accept friend requests. I actually want to know the people I friend here."The world is full of suffering.. It is also filled with the overcoming." - Helen Keller"An eye for an eye makes the world blind." - Gandhi"The best and most beautiful things in the world cannot be seen or even touched; They must be felt with the heart" - Helen Keller"The best place in the world is in the arms of someone who will not only hold you at your best, but will pick you up and hug you tight at your weakest moment" - Unknown
No favourite stories listed.
Not following any authors
Personally not a fan.. I know many use it in a positive manner and don't mean anything negative at all when using it, but for me it brings negative associations anyway, so I prefer not being called it or similar words.
Time off from work, cuddling with my doggies and knowing I am loved.. :)
January 31st, 6pm CET
Erm... good question... don't truly know yet.. but... many... blushes..
BothI cover her mouth with my kiss, I uncover her secrets with wandering fingertip.I cover her breast with my palmI uncover her dreams with a listening earI cover her back with my flogI uncover her beauty with awakened desiresI cover her body with my ownI uncover her soul with softly spoken words This... Exactly this.. So beautiful..
There are very few absolutes within this lifestyle. How it is lived, has about as many varieties as couples within it. Besides the parole of "Sane, Safe and Consensual" there are few or no absolutes on how to practice BDSM. So what is the lifestyle all about?There is one core thing that holds true for all the dynamics within BDSM; It involves some sort of power exchange. To put it in a simplified way, one person (the Dom) has power over the other (the sub). But here comes the crucial part; The power should always be GIVEN FREELY by the sub, in the manner decided by the sub. A real Dom will NEVER take or force power from a sub. The sub sets the limits within the relationship and can stop things and ask for the power back within a second. Once the dynamics have been set/decided, the Dom holds power over the sub, but the power should ALWAYS flow from the bottom (the sub) to the top (the Dom). A Dom only uses the power freely given to him/her by the sub based on his/her needs and wants.It is also important to understand that being a Dom or sub is part of your personality, part of who you are, not a role you play. Much like say your sexual orientation is part of who you are, so is being a Dom or sub. BDSM isn't about sex, in fact you can have BDSM relationships without any sex at all. However, most BDSM relationships will have a sexual component as most relationships between adults do, whether they are D/s or vanilla.These are the basics of BDSM as I see it. Without understanding these, it is impossible to know what the lifestyle is about. There are tons of different dynamics in BDSM, but these things holds true regardless. Anyone else have any thoughts on this and how to best explain the lifestyle to those trying to learn about it and understand what BDSM is?
Happy birthday!
31 please
I would like some more backgrounds too, some new colours etc. Fun with some new things :)
I never meant to imply that a D/s relationship is better than a well-functioning vanilla relationship. A great relationship is a great relationship, whatever "category" it may be placed within.Of course you leave yourself vulnerable in an relationship where you truly connect and open up to another person. It is impossible to enter any form of meaningful relationship and guarantee you won't get hurt. I will however, based on personal experience with both vanilla and D/s-relationships, stand by than for the most part subs leave themselves extra vulnerable. The reason I think so, is that a sub usually gives over more control to her/his partner than someone does in a vanilla relationship. By control in this context I mean things like structuring of every day life, tasks given etc. The emotional hurt can be the same from any type of relationship, but it is easier for a sub to "spin out of control" as they are relying more on their partner. (I am not saying you don't rely on your partner in other forms of relationships, of course you do, but the whole point within D/s is that more control is given over than in other relationships).Yes, I have been hurt before and I have learned a lot from it, good and bad. Has it left me more guarded? In some ways yes, and from learning and experiencing you are better equipped to deal with things you go through in the future, but you are also left with some scares that might never go totally away. We all make mistakes and hopefully learn from them. We all need to crawl before we can walk, walk before we can run. I see again and again tho those wanting to run before they can even crawl, not being honest with themselves or others where they are at in "their development". Honesty is the key I think in most circumstances. Of course there are some that are just pure abusers and they are often extra attracted to a BDSM lifestyle as it can be easier to "hide" abuse there, but that is really a whole other topic in itself.
"He loves me, he loves me not, he loves me, he loves me not…" She smiles secretly, playing the game of childhood years, Petals of the daisy falling to the ground. The evening air still warm As she waits for him, He who makes her heart race, Her breath tremble and core moisten. "He loves me, he loves me not, he loves me, he loves me not…" She has loved and lost, But he makes her...
Added 09 Jul 2016 | Category Love Poems
| Votes 13 | Avg Score 5
| Views 339
| 14 Comments
Heartache. Heartbreak. Lost and broken On the floor, Not knowing where to go, Where to turn, How to breathe. Friends gathering, Showing support and concern, Giving space, yet staying near, Ready to lend a hand When I feel ready To get up and take a deep breath, Move on. Working on finding a place Where pain is not the only feeling, Somewhere each step Will be a bit easier...
Added 05 Jul 2016 | Category Love Poems
| Votes 5 | Avg Score 5
| Views 177
| 6 Comments
The quiet hum of bees Busy collecting nectar To make their sweet honey, Surrounds her as she lays down in the grass, The gentle morning sun caresses her skin, Warming it as a lover's touch. Birds sit in the trees Singing their cheerful songs, Welcoming a new day Filled with opportunity and promise. He stands and watches her, Not wanting to disturb a quiet moment. Admiring the...
Added 03 Jul 2016 | Category Erotic Poems
| Votes 3 | Avg Score 5
| Views 282
| 3 Comments
Set me free From this prison Of my own emotions, Locked behind bars Constructed by pieces Of a broken heart. Caged by the chaos Raging in my mind, Feelings shattered Like broken glass, Mirroring the sadness Tormenting my soul. Hopes and dreams Crushed into nothingness, Small specs of dust Forever blown away By storms raging Deep within me. Trapped by sorrow, Longing for...
Added 01 Jul 2016 | Category Love Poems
| Votes 4 | Avg Score 5
| Views 280
| 4 Comments
The night is dark and quiet, The world sleeping. Alone, she lays, Curled up in her bed, Longing for the warmth of her lover, His arms around her, Naked skin against skin, Feeling protected and safe. She longs to have him near, But she knows tonight, He will not calm her aching, She has to find a way To quieten down, relax, On her own. How can simple sleep Be so hard to find? ...
Added 21 Jun 2016 | Category Erotic Poems
| Votes 5 | Avg Score 5
| Views 578
| 5 Comments
Filled with aching need, She presses the curves of her form Tightly against his contours. Feeling radiating heat through her clothes Awakens a smoldering fire within him, Hands ripping off items in their way, Leaving naked skin against skin. His hands graze softly across her back, Sending sparks of electricity shimmering through her being, Mouths crashing together, Hot breaths,...
Added 01 May 2016 | Category Erotic Poems
| Votes 5 | Avg Score 5
| Views 697
| 7 Comments
A song plays over the speakers One of their songs And he smiles at her Whispering "Dance with me?" Eyes A little bit sad Meets his "You know I can't" He looks at her, smiling Lifting her up Into his arms Whispering "Yes you can" On the dance floor He softly sways With her in his arms As they dance in their own way Eyes meet Gazing Deep into each other A silent knowledge...
Added 25 Apr 2016 | Category Love Poems
| Votes 3 | Avg Score 5
| Views 452
| 5 Comments
I have been "not enough.." I have been "too much.." I have been "if only.." But deep within my very core I always longed to be "just right.." Becoming his opened my eyes To something new, Made me see what I needed, What was possible, Giving me what I thought only lived in dreams. He touched me and my body ignited, Flames flickering over my most intimate parts, Want, need, a...
Added 21 Apr 2016 | Category Love Poems
| Votes 5 | Avg Score 5
| Views 710
| 6 Comments
She wasn't looking, Not expecting anything, When she found him, Or perhaps he found her? Maybe it was meant to be, Maybe a mere coincidence, Whichever, it changed everything. He broke down the walls Around her fragile heart, Tearing apart pain left by others, Erasing the power Of old words spoken, Leaving them meaningless, Replacing them with his own. He simply wanted her, ...
Added 06 Apr 2016 | Category Love Poems
| Votes 11 | Avg Score 4.91
| Views 783
| 11 Comments
With trembling hands, Passion burning in her eyes, She runs her hands over him, Exploring his naked beauty, Fingertips dancing across skin, Lips kissing, nibbling, tasting. Her body burns with desire, Hungers to feel him inside, Have their bodies unite Drawing pleasure from each other, Move as one, Drown in the shared lust. Straddling him, their eyes lock. For a moment time...
Added 01 Apr 2016 | Category Erotic Poems
| Votes 3 | Avg Score 5
| Views 896
| 4 Comments
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Klaus Knopper answers your Linux questions
Ask Klaus!
Klaus Knopper is the creator of Knoppix and co-founder of LinuxTag expo. He currently works as a teacher, programmer, and consultant. If you have a configuration problem, or if you just want to learn more about how Linux works, send your questions to: email@example.com
Bad DVD or Drive?
Hello Klaus, I have been using Knoppix DVDs from Linux Magazine for some time and have had no problems with versions 6.1 to 6.5. However, I recently tried version 6.7 and it gives the message in Listing 1, so I tried knoppix testcd and it does the same. I concluded that I may have a dud DVD and decided to wait for the next release.
When Knoppix 7.0 came out in Linux Magazine, I tried again, and got the same message. Now, it is highly unlikely that two DVDs are defective in the same spot, so I tried further boot options – nodma, acpi=off, and failsafe – to no avail. I note that versions 6.7 and 7.0 of Knoppix use compression. Is that the problem?
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Mirantis Joins Hands with SUSE To Offer RHEL Support; Red Hat Not Happy
Competitors get in the game with RHEL without Red Hat
Linux on Windows 10 Poses a Security Risk
Security researchers have already notified Microsoft; some fixes are available
Mirantis to Refactor OpenStack Fuel for Kubernetes
The company is collaborating with Google and Intel to use Kubernetes as an engine for Fuel
Canonical Joins Document Foundation Advisory Board
The upcoming release of LibreOffice will be available as a snap package
SUSE Joins the Microsoft Test Drive Program
Customers can take a free test drive of SLES for HPC on the Azure Cloud
SiFive Launches a Line of Open Source Chips
San Francisco-based chip company announces their first fully open source chip platform.
Fedora 24 Released
The whole distro gets rebuilt on glibc 2.3
Canonical Announces Cross-Distro Support for Snaps
Ubuntu Vendor tries to solve app packaging and distribution problem across distributions.
ownCloud Forked
Founder of ownCloud launches the Nextcloud project.
HP Enterprise Unveils a Futuristic Computer
Will The Machine change the way future programmers think about memory?


text: Logitech G9x Wired Laser Gaming Mouse + Free Star Trek game w/ purchase Your Price: $54.99 With Promo Code EMCXSTX59 Flag this deal What's the matter? Choose a reason Dead deal Duplicate Bad link Spam Inaccurate No value Alive again Edit deal sly1960 Apr 30, 2013 great deal, a must have..thanks for post! Reply Load More Comments


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Book your room at Days Inn Portland
Get an overview of this hotelPortland hotel in North Portland, walk to Portland Meadows Racetrack
Located in North Portland, this hotel is 0.4 mi (0.7 km) from Portland Meadows Racetrack and 1.1 mi (1.8 km) from Portland International Raceway. Portland Expo Center and Woodlawn Park are also within 3 mi (5 km).
Hotel Features
This hotel features a 24-hour fitness center, coffee/tea in a common area, and a meeting room. Free continental breakfast is provided, as well as free WiFi in public areas, free self parking, and a free airport shuttle. Additionally, laundry facilities, a 24-hour front desk, and a computer station are onsite.
Room Amenities
All 210 rooms offer free WiFi, coffee makers, and TVs with satellite channels. Other amenities available to guests include free weekday newspapers, free local calls, and hair dryers.Would you like to give us feedback? Share it! Opens in a new window
Days Inn Portland9930 North Whitaker Rd. Portland OR 97217
Renovations & Closures
Hotel Amenities
Hotel Amenities
Guests can enjoy a complimentary breakfast each morning. A computer station is located on site and high-speed wireless Internet access is complimentary. Event facilities measuring 850 square feet (79 square meters) include a meeting room. This Portland hotel also offers a 24-hour fitness center, coffee/tea in a common area, and complimentary newspapers in the lobby. Complimentary self parking is available on site.
Days Inn Portland has designated areas for smoking.
- 24-hour fitness facilities
- 24-hour front desk
- Free airport transportation
- Coffee/tea in common areas
- Free newspapers in lobby
- Computer station
- Conference space size (feet) - 850
- Conference space size (meters) - 79
- Designated smoking areas
- Elevator/lift
- Fitness facilities
- Free breakfast
- Free self parking
- Free WiFi
- Grocery/convenience store
- Laundry facilities
- Luggage storage
- Number of buildings/towers - 1
- Number of floors - 4
- One meeting room
- Safe-deposit box at front desk
- Television in common areas
- Total number of rooms - 210
Family Friendly Amenities
- Cribs/infant beds (complimentary)
- Free Wi-Fi
- Grocery/convenience store
- Laundry facilities
Available in all rooms: Free WiFi
Available in some public areas: Free WiFi
Free self parking
Room Amenities
- Air conditioning
- Blackout drapes/curtains
- Coffee/tea maker
- Free toiletries
- Free weekday newspaper
- Connecting/adjoining rooms available
- Free cribs/infant beds
- Daily housekeeping
- Desk
- Free local calls
- Free WiFi
- Hair dryer
- Phone
- Premium TV channels
- Private bathroom
- Satellite TV service
- Shower/tub combination
- Television
Where to Eat
A complimentary continental breakfast is served each morning between 6:30 AM and 9:30 AM.
Nearby Things to Do
- Golfing nearby
To make requests for specific accessibility needs, complete the "Special or Accessibility Requests" section of "Trip Preferences" when you book your hotel.
- Accessible bathroom
- Roll-in shower
- In-room accessibility
Hotel Policies
Minimum check-in age is 18
Check-in time starts at 3 PM
Check-out time is noon
Payment types
- Pets allowed
You need to know
Extra-person charges may apply and vary depending on hotel policy.
Government-issued photo identification and a credit card are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
Optional extras
- Rollaway bed fee: USD 10 per night
Hotel Name
- Days Inn Hotel Portland
- Days Inn Portland
- Portland Days Inn
- Days Inn South Portland Hotel South Portland
- Days Inn South Portland Maine
- South Portland Days Inn
- Days Inn Portland Hotel
We should mention
The property has connecting/adjoining rooms, which are subject to availability and can be requested by contacting the property using the number on the booking confirmation.


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YORK, Maine — Virginia A. Butterworth, 83, of 161 York St., and a longtime resident of Hampton, N.H., died Monday, Nov. 5, 2012, at Portsmouth Regional Hospital.
She was born Sept. 30, 1929, in Lawrence, Mass., a daughter of the late Ernest and Margaret (Shattuck) Zecchini.
Virginia had worked as a waitress for Jerry's Restaurant in Hampton for 25 years.
She leaves two sons, Bruce M. Butterworth of Seabrook, N.H., and David S. Butterworth of Farmington, N.H.; four grandchildren, Lisa, Nicole, Chad and Corey; and two great-grandchildren.
Her husband of 58 years, Alfred E. Butterworth, died June 5, 2009.
SERVICES: A memorial service will be held at 11 a.m. Saturday, Nov. 10, in the Lucas & Eaton Funeral Home, 91 Long Sands Road, York. There are no calling hours. Memorial contributions may be made to American Cancer Society, 30 Speen St., Framingham, MA 01701. Visit www.lucaseatonfuneralhome.com.
Location, ST | website.com


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A Zanesville man convicted in the murder of a 5-year-old area girl will stay in prison.
Whiz News has learned that Billy Joe Shafer has been denied parole in the aggravated murder of Sara West. The Ohio Parole Board held a hearing for Shafer Thursday.
Board Spokeswoman JoEllen Smith says Shafer was denied parole and will have to wait until 2020 to have another hearing.
Smith says the decision was actually a recommendation and won't be final for a couple of days. It...


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And now it won't let me access anything. It shows the front page, but without CSS and links are not working. When I try to log on i reach the log-in window (without CSS) but as I log in I get Object not found.
How can I revert this?
Site is live. Quick replies are extremely appreciated.


text: Breakfast Sandwich in 90 Seconds December 18, 2012 by Lindsay 92 Comments Hi Friends! It's time to talk about the breakfast sandwich. So, here's the deal. My husband's not a breakfast eater. Never has been. I am. Always have been. We've been together for almost 10 years and I still have yet to convert him. BUT, we're ...Read More »


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Pressing the 'Optimized content gallery' tab from my Settings brings me here:
Which displays the ordinary site & theme instead of admin panel, and returns this message:
Sorry, we can't find the content you're looking for at this URL. Please try selecting a menu item from above or to the side of this message to get where you'd like to go.
Looking at the old plugin you've forked this from I'm very excited to see how this turns out though! :)


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Joshua got me this loom the Christmas before last. A lovely rigid heddle loom for the ever-dreaming-of-new-projects-whilst-never-mastering-any person that it turns out I am. I didn't realize this was when I was younger. I called what I experienced eager curiosity. Named it willingness to try anything new.
But as the years have passed, I've started to wonder about these urges. Urges that have me buying skeins of yarn for projects I've seen but can't sit myself down to figure out long enough to make something of them. Passionate impulses around fabric I may never sew into something. And sometimes I've even recognized a piece of jealousy hitching along for the ride. A bit of what I see clearly in my own daughter who enthusiastically says in all honesty, If she can do it, so can I!
So, I'd fallen in love with the idea of weaving. And I actually tricked myself into thinking it would be simpler than crochet or knitting, which still baffle me with their intricate patterns written in secret-I-can't-slow-my-brain-down-long-enough-to-decipher-code.
Yes, long swaths of weaving, back and forth, that might be a better fit for my Virgo-esque desire for creative repetition. For setting up a loom and then coasting in autopilot as you put the thing together sounds a lot better than working a little, stopping to follow directions, working a bit more, checking in again, and so on.
Boy was I fool.
The first night the loom and I met, I struggled to figure out how to get the "simple" pieces put together and tied on. Husband came to my rescue and helped me get it all set up. And I became dependent on the second brain in the room. What is this saying? I begged him to read on and on with me.
The second night I energetically pulled out the loom, dreaming of weaving by bedtime, I ended up realizing there's a lot more to warping than I'd imagined. I felt like barfing as I read and re-read the directions making no sense of each and every line of text in Learning to Weave. I felt defeated.
I took out Creative Weavingand breathed in the rich pages of color, warp and weft balancing everything out again.
And then every time I thought about taking the loom out again, I didn't feel like it.
For a whole year.
It sat under my bed, clouds of woolies floating freely around it's lovely box. Vacuum gently nudging it each and every week as I bustled about attempting to ignore the feeling of dread it was causing me to consider another try in setting it up.
But eventually, birthdays celebrated, Christmas and solstice past, I finally drug out the Glimakra box. Warily, I'll admit.
And then it was time to figure this beast out. So I sat again, the kids' bedtime fading into early morning hours. And this time I swear I was truly ready to throw up. The warp was @#&#ed up. The knot tying was hideous. And I finally took my scissors out and was just about to cut the whole thing off the loom when Joshua gasped. Really?!
Thank goodness for that inhalation. I sat and stared a bit longer and then re-attempted tying the warp off. And an hour later had something that looked alright.
And so here it is at long last. The first project. On it's way now.
And what have I learned so far?
1. I still suck at word problems. Mental incapacity to read and make sense of anything in a productive sense.
2. I do love yarn, but have a lot to learn about it.
3. I must get better at winging it and making mistakes along the way. Never was good at doing it gracefully but this new craft if forcing me to do it respectfully.
4. Which leads to my picking the wrong heddle. I'm living with that choice each and every stroke made. Darn it but know that I won't make that mistake again.
5. My brain is NOT too old to learn new tricks. Yay for that lively feeling of finally getting this thing going.
And so, this week, I feel balanced. Both because I'm managing to escape to my bedroom midday for a few new rows of weaving as I soak up some sunlight AND because life is full but in all the right places at this moment in time.
Now, bring on the rain Northern California! We're ready for it. And the garden too. Which was my inspiration for this table runner. Thrifted yarn that saved the day!
Ahem. Anyone else happen to be a weaver? Heck, anyone with a love for the crafts, Cheers to creative energy!


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Field & Stream Kids' Woodsman Waterproof 400g Field Boots
Product Information:
They'll live up the season in the Field & Stream® Kids' Woodsman Waterproof 400g field boot. Providing durability with leather and a nylon canvas upper, this boot features waterproof technology that keeps them dry and water at bay. 400 grams of Thinsulate® insulation delivers plush warmth, while a high-quality TPR outsole offers excellent support and traction. With excellent performance, the Woodsman boot will last them season to season.
- HydroProof ULTRA waterproof and breathable technology keeps water out so they stay dry
- 400g Thinsulate® insulation provides warmth even in the coldest conditions
- Leather and nylon canvas upper provide generous support and long lasting comfort
- EVA sockliner for plush cushion with each step
- Durable TPR midsole and outsole delivers traction control while offering excellent arch support
- Style: FASRBY034-RTX
- Field & Stream
Country of Origin: United StatesWeb ID: 35119626
Customer Reviews:
Rated 4.6 out of 5 by 5 reviewers.
Rated 5 out of 5 by Firetrixee Great quality!! These boots are well made and perfect for kids! Would definitely recommend! November 12, 2015
Rated 3 out of 5 by Jen Field &Stream Kids My son loved them. But after day two of use them you could see there places where not glued properly. November 11, 2015
Rated 5 out of 5 by ksv This product is great It fits perfectly and the price wasn't that bad. I recommend this shoe. February 26, 2015
Rated 5 out of 5 by Melli21 Great boots Bought for my son. He loves them! Very warm. Worth the price January 3, 2015
Rated 5 out of 5 by coaltrain Good sound boot Boot was a perfect fit for my Grandson. He loves them and got to enjoy them first time out by shooting his first spike buck. Just the right amount of insulation for all-around wear. Not bad for an 8yr. old boy. October 30, 2014
1-5 of 5


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The right to the City - Bridging the urban divide World Urban Forum, 22 - 26 March 2010, Rio de Janeiro
The upcoming World Urban Forum, established by the United Nations in 2002, examines one of the most pressing problems facing the world today: rapid urbanisation and its impact. Concepts that will drive the discussions at the fifth WUF session in Rio include the right to the city, bridging urban income gaps, reducing inequality and poverty, participatory democracy, cultural diversity in cities, women-friendly cities, sustainable urban development equal access to shelter, health, water, sanitation and infrastructure services.
Mark Napier and Jonathan Diederiks from Urban Landmark will attend the conference and report back on the proceedings in the next issue of this newsletter.


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Wednesday, Feb. 12, 2014
CSRA (WRDW) - We're On Your Side with updates throughout the day on weather and road condition in the CSRA.
You've been on our side sending us pictures of what's been happening in your own backyard.
Here's a slide show of some of the pictures we've gotten from you.


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iPhone News
Filtered Results
New Pokemon GO update lets you know which Pokemon can be the very best
Learn which Pokemon have the most potential.
Mr. Robot game out now on iOS and Android
Interactive chat app experience created by Oxenfree dev Night School Studio, and Telltale.
Pokemon GO has 5.3 million active adult UK players
That's an impressive 87% of those who downloaded the app.
Pokemon GO's latest update introduces a working Nearby system, but only for selected players
Step in the right direction.
Niantic gives perfectly reasonable reason why it shut down third-party Pokemon GO apps
They were hammering the game's servers and preventing the game from launching in additional territories.
3DS sales up by more than 200 per cent in the UK
Pokemon GO sends fans in search of more Pokemon titles.
Telltale says Batman PC issues largely down to integrated graphics
Out of date drivers also fingered, but new patch aims to help.


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|Living with Adult ADHD
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Success in High School/Problems in College
"My 21-year-old son graduated at the top of his high school class, but now can't seem to make it in college."
The primary question is whether his untreated ADHD caused his college difficulties or whether something else was involved. If he says that he tried to concentrate in class and when he studied but could not, there is the possibility that his problems were because of his being off medication. Hopefully, the experiences of the first year in college will convince him to consider going back on medication. If he does want to, there are at least eight medications to consider. Ask him to discuss this with his physician.
You must consider another possibility. For a high percent of freshman, the problem is the freedom and lack of supervision. It is very common to prefer social life to study. Alcohol and/or drugs might become a daily or every weekend activity. Soon, classes are missed and work is not done. Sadly, about 11 percent of freshman college students drop out because of these problems. Discuss this possibility with your son before concluding that it was being off of medication.
Larry Silver, M.D., is clinical professor of psychiatry at Georgetown Medical Center in Washington, D.C. and director of training in child and adolescent psychiatry at Georgetown University School of Medicine. He is a former acting director and deputy director of the National Institute of Mental Health, as well as the author of Dr. Larry Silver's Advice to Parents on AD/HD and The Misunderstood Child: Understanding and Coping with Your Child's Learning Disabilities.
What do you think of this article? Share your comments on www.ADDConnect.com, ADDitude's community site. Check out the new ADHD Medication User Reviews and the ADHD Adults Support Group. Your fellow ADDers want to hear from you!


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Giuseppe Zanotti Design
fur detail hi-top sneakers
Out of stock
Unfortunately this item is out of stock
Black leather fur detail hi-top sneakers from Giuseppe Zanotti Design featuring a round toe, a lace-up front fastening, side zip fastenings, gold-tone hardware, contrasting panels and a white rubber sole.
Made in Italy
Designer Style ID: RW4050Z01
Farfetch ID: 10767366
- Measurements:
- wedge: 2.5 centimetres, height: 11.5 centimetres
- Composition:
- Suede 100%
- Outer Composition:
- Leather 100%
- Sole Composition:
- rubber 100%
Your order will be shipped directly from the boutique to you. Farfetch ships worldwide, and you will be able to track your order at any time. The cost of shipping your order will be calculated at the checkout.
Import duties information
Delivery duties are included in the item price when shipping to all EU countries plus USA, Canada, China, Australia, Puerto Rico, Switzerland, Singapore, Republic Of Korea, Kuwait, Qatar, India, Norway, Saudi Arabia, Taiwan, Thailand, U.A.E., Japan.This means all import duties are included in your order, so the price you see is the price you pay. You will not receive an additional duty bill from the courier to receive your shipment. If you are ordering from within your own customs zone or country, import duties will not be applicable anyway, however you may have to pay VAT or local Sales Taxes where applicable (for the UK and USA these are included at the checkout).
Delivery duties are not included in the item price when shipping to all other countries. This means you may be liable to pay import duties on receipt of your order.
Free returns pick-up
You have 14 days from receiving your order to return the item to the boutique using our free returns pick-up service. We strongly recommend that you book your free returns pick-up within 7 days of receiving your order to ensure that it arrives back to the boutique in time. Please note for standard orders we can only collect returns from the same country to which your order was delivered. For click and collect orders we can collect your return from the country your order was delivered to and also the country of the original boutique you ordered from. Click here to read our full returns policy.
Giuseppe Zanotti hails from the Italian shoemaking town of San Mauro Pascoli and his career has seen him design shoes for luxury labels including Dior and Roberto Cavalli. His super-glam evening shoes have become red-carpet staples.
Cambridge , United Kingdom
Sitting in the shadows of the beautiful colleges and spires of Cambridge University, longstanding Giulio feels almost as iconic. The boutique is split into five rooms, each showcasing a different category of luxury, casual and contemporary fashion, and the eponymous owner has as much character as the clothes he sells.


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"Home Styles Bedford Lingerie/Jewelry Chest - Black" is not available for sale online.
This item may be discontinued or not carried in your nearest store. Please call your local store to check in-store availability before heading there. For informational purposes, the product details and customer reviews are provided.
- Additional bottom three drawers provide ample space for clothing or lingerie and also feature sides that open to felt-lined compartments with hooks for more jewelry.
- Hardwood/engineered wood.
- Measures 50" H x 22" W x 16" D.
- Easy to assemble.
- 30-day manufacturer's warranty.
- Imported.


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CategoriesBach Baptism Books Catechesis Chemnitz Children Church Year Confirmation Devotions El Paso Evening Family Food and Drink Grandpa Home Hospital Calls Hymnody iPhone Luther Lutheranism Marriage Military Ministry Music Prayer Quotations School Seelsorge Study Symbolics Tech Trinity Uncategorized Worship Writing
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Monthly Archives: July 2011
A Faithful God—From Generation to Generation
The story goes in my family that my great-grandmother, Anna Schindeldecker Linkert (pictured right), sang to her mother as she was dying. According to the story, she sang the stanzas of Johann Heerman's hymn, O Gott, du frommer Gott (O … Continue reading
Over a month ago I bought myself an Amazon Kindle. For those of you who haven't seen these or heard of them, the Kindle is an electronic book-reading device. I should mention that this decision was a bit of a … Continue reading
Worship Conference
There are no words which can adequately describe the experience of attending the WELS National Conference on Worship, Music, and the Arts. The best I could do would be to encourage you to view online the two services which were … Continue reading
Posted in Children, Hymnody, Worship Leave a comment
Let me depart this life Confiding in my Savior Do thou my soul receive That it may live forever And let my body have A quiet resting place Within a Christian grave And let it sleep in peace.
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Hymns and Children
Yesterday I heard an interesting segment of Issues, etc, on teaching hymns to children. You can listen to the segment here. This covers some of the same ground that I will be presenting in a few weeks at the WELS … Continue reading
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Black Crusade: Core Rulebook
Warhammer 40k RPG
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Product Description
The intoxicating lure of Chaos leads untold millions down the path of corruption, and the noblest of intentions offers little protection against its dark influence. A soldier's bloodlust, a politician's ambition, a lover's desire, and even a mother's whispered prayer over her feverish child – they are all the playthings of the Dark Gods. Why resist? Wealth, power, and happiness await those who serve the Ruinous Powers, and the only price is your humanity.
Black Crusade is a remarkable standalone RPG that offers players an entirely new perspective on the conflict between the Imperium of Man and the forces of Chaos. This groundbreaking concept delivers the unprecedented opportunity to play as a Disciple of the Dark Gods, whether as a Chaos Space Marine or a human Servant of Chaos.
Swear allegiance to your dark masters, and reap the rewards of their favor! The Black Crusade core rulebook contains everything you need to start your adventure in the Warhammer 40,000 universe.
Product Information
Asmodee North America, Fantasy Flight Games
Year: 2014
Weight: 1,545 grams
Warhammer 40k RPG
Warhammer 40k RPG