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FILE - In this Feb. 14, 2013, file photo, House Speaker John Boehner of Ohio arrives to meet with reporters on Capitol Hill in Washington. Republicans and other fiscal conservatives keep insisting on more federal austerity and a smaller government. Without much fanfare or acknowledgement, they've already gotten much of both. (AP Photo/J. Scott Applewhite, File)

subset: commoncrawl


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Tuesday August 9, 2011
Edgefield, SC--If you happen upon a Wardlaw Academy practice now-a-days you'll probably be able to see the smiles from the road. Winning is the norm for the Patriots and after a tough season last year, this is a squad loaded for bear.
But these guys are the bears and its starts up front. A huge front line that you don't see everyday for eight man football teams. Heck, they go on average around 240. Throw in all sorts of experience in quarterback Bailey Pendarvis and running back Jordan Faust, and they're going to be good. There's also a little payback they're ready to dole out.
"It does, we look forward to meeting that team we lost to again," says Pendarvis. "We've been thinking about it, and we know we got to work hard, get in shape so we can go hard so we can finish good this year."
"We've had a great summer program, and our guys have been in the weight room since last spring," says Coach Chalmers Carr. "They worked out all summer long but yeah, they have gotten bigger. I tell you what, I'd like to be a running back behind these guys!"
All told, they only lost two seniors a year ago and now have seven on a squad of 17. Between the size, speed and skill, these kids are ready to add another state title to a program use to hoisting a trophy in the last game of the year.


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Image credit: wikimedia commons.
I found actually widgets for blogger that show most popular pages. One is from feedjit shows most visited articles during a day. There are several implementations of popular post widgets that show popular posts by number of comments, such as this one, which works with google pipes. Now, in my opinion, one day of traffic doesn't really give too much statistics to give useful information about which of your pages are most popular. As for the other widget, I don't want popular posts by comments, I want just the most visited ones. There is postrank, which shows popular posts measures of post submission to social bookmark sites such as slashdot or digg, however it would be nice to have a widget that shows most visited pages.
The widget I present here uses google analytics statistics to list most viewed posts. You'll need a google analytics account and the tracking code active on your site for it to work.
Installation of this widget is similar to the previous popular post widget with feedburner.
You can host the code on any site, however in this walkthrough I assume you use google app engine. If you don't have one already, you need to create a free account on google app engine. Hosting your files and web applications on google app engine brings you the advantages of a low latency content delivery network (CDN) which can make your site load faster and for this implementation of the most popular posts widget you need an account. You'll have to choose a domain such as yoursubdomain.appspot.com.
If you don't have it already install python. Download the app engine python SDK for python.
Download the source code of the widget. You need subversion for this. The command for checkout is this:
Go to the popular-posts-for-blogger/popular-posts-for-blogger subdirectory and edit the getfeedstats.py file (use an editor or IDE that knows python, such as eclipse, vim, or kate). Search and replace myoutsourcedbrain for your feedburner uri.
Upload your code with the google app engine SDK. Still in the same directory type:
If you did everything correctly you should now be able to see the javascript at http://yoursubdomain.appspot.com/reports/analytics.
In your website or blog, where you want the widget to show, paste this code:


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Built from hard acrylic, EarSonics EM2-PRO is the sonic reference in terms of neutrality and high fidelity sound. Our double-transducer system produces a very musical experience with an incredibly wide frequency response, a deep and strong low-end, clear mids and an incredible high range finesse. Earsonics EM2-PRO provides 28 dB of passive ambient noise reduction, unmatched clarity, precision and dynamic. For the "high fidelity sound" enthusiasts.
Chose your color options: cord and shell
Select your optional laser printing (send us a mail with your logo at email@example.com)
Send us your ear impressions at EarSonics, Zae Via Domitia, 172 av des gardians 34160 CASTRIES, FRANCE)
Prices are displayed inclusive of EEC tax, to see the price excl tax (for orders from outside of EEC) you have to log yourself in with your original country.


text:
Optimism turns to despair as NHL talks break off
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Product Description
The Wine Clip uses principles of magnetics to improve the taste of wine as it is being poured out of the bottle. The effect is instantaneous, and has been found by many wine professionals to result in a genuine improvement in flavor and mouth feel, especially when used on red wines. Wine experts agree that The Wine Clip can enhance the taste of wine, making it smoother, less bitter and more refined. Simply attach The Wine Clip to the neck of your wine bottle and pour. Instantly, you'll experience.A smoother, less-tannic taste. A more pleasant aroma.A more enjoyable wine experience.It doesn't matter whether you're a connoisseur or a casual wine drinker, whether you're opening a $10 bottle of merlot or a $300 bottle of cabernet sauvignon. Attach The Wine Clip before pouring and your wine will taste smoother, more refined and infinitely satisfying. In blind taste tests with more than 1,000 participants, the result is always the same: wine is more enjoyable when accompanied by The Wine Clip. Give yourself the experience. The Wine Clip is warranteed for a lifetime.The Wine Clip may also accelerate aeration, by drawing higher concentrations of oxygen to the wine as it is being poured.


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Your personal safety does not have to be left up to chance. Though you can not eliminate all possibility of becoming a victim, there are steps you can take to reduce the odds. An attacker looks for essentially three things when picking a victim:
Practicing the following personal safety tips as you go about your daily activities may make you less attractive to a would be criminal.
For additional information please review the following personal safety tips:
If you have any questions concerning crime prevention topics please contact your email@example.com.


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text: Basketball Jam: Students vs. Marian University Staff/Faculty Come watch as an all student team takes on an all staff/faculty team in this annual basketball show down. Make no mistake, this game gets competitive! Last year the faculty/staff team won. The students will look for revenge this year. Cost: Free! Location: Physical Education Center, Main Gymnasium Time: 7 p.m.


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The Nonprofit Social Media Decision Guide
UPDATED for 2013...
Nearly a decade after Facebook launched, it's become a juggernaut with more than a billion registered users—and the field continues to grow more crowded as other social media channels burst onto the scene. There's little doubt that social media can and should be a powerful part of your communications mix, but what should be using it for? Outreach and engagement? Event management? Advocacy? How about fundraising? For many nonprofits, it's far more obvious that such tools can be useful than how to use them.
We created The Nonprofit Social Media Decision Guide to help organizations like yours determine what results and benefits you can reasonably expect from social media, and to guide you through the process of identifying the right channels for different goals. To help you turn the theoretical into the practical, we've included a workbook that lets you apply what you're learning to your own real-world needs. For this year's edition, our second update, we've added new research on how nonprofit organizations are using social media to meet their missions, and updated our overview of key differences between the channels most often used by nonprofits to reflect their current functionality. We've also added sections covering new and up-and-coming social media platforms.
From tool-by-tool comparisons and strategies for success to ways to integrate multiple channels, the guide is built upon extensive research, interviews with experts and other users, and our own broad experience. We've also included case studies of organizations successfully using social media for different purposes. (We're also offering an Advanced Social Media Decision Maker's Toolkit course for hands-on training to help you form a more complete view of your whole social media picture.)
Social media is a conversation the world is having. Your organization can't afford to be left out of it. Let Idealware's Nonprofit Social Media Decision Guide help you find your voice. Like all our reports, it's free to download. Just sign up below.
The updated 2013 Decision Guide covers:
- What social media is, and why it might be useful.
- How goals, audiences and metrics drive decision-making.
- Specifics on the tangible value of Facebook, Twitter, Blogs, Photo- and Video-Sharing Sites, and more.
- Choosing an effective social media mix.
- Integrating your communications.


text:
Red team rolls in UWA spring game
Published: Sunday, April 7, 2013 at 3:30 a.m.
Last Modified: Saturday, April 6, 2013 at 11:59 p.m.
LIVINGSTON | The University of West Alabama Red Team rang up 420 yards of total offense, including 281 combined receiving yards by Chad Toocheck and Malik Lofton, on the way to a 28-10 victory over the White Team as the defending Gulf South Conference champions conducted their annual spring game Saturday at Tiger Stadium.
"Hopefully we are going to be more explosive offensively, not quite as much ground and pound," UWA coach Will Hall said. "You saw us putting the ball in the air more today. We are trying to improve our passing game after leading the GSC in rushing the last two years."
Toocheck caught seven passes for 147 yards, including a 64-yard touchdown from junior quarterback Kyle Caldwell on the first play of the second half. Lofton had six receptions for 134 yards that included a 40-yard scoring hookup from Caldwell.
Caldwell finished the day 14-for-22 for 262 yards while taking snaps for both teams. Mid-year junior college transfer Clint Hatten also took snaps with both teams, completing 9 of 15 for 178 yards. Jeremiah Chandler was the only other quarterback to see action, completing 1 of 6 for five yards.
Sophomore running back Javae Swindle rounded out Red Team scoring with runs of 2 and 1 yard, carrying 12 times for 38 yards. Hatten scored from 1 yard out and Mark Grant connected on a 23-yard field goal to account for the White Team scoring.
"I feel good about where we are, but I was not pleased with our kicking game," Hall said. "Our kickers have been really good all spring, but you get out here today with people in the stands and a little pressure and we missed some."
Jordan Ikner missed a 47-yard field goal attempt and Grant hit the right upright from 28 yards.
The Red Team defense limited the White to 201 yards total offense and 2 of 11 third-down conversions. Frank Richardson intercepted a pass and Dereck Jordan picked up a fumble and returned it 18 yards.
Reader comments posted to this article may be published in our print edition. All rights reserved. This copyrighted material may not be re-published without permission. Links are encouraged.


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Guo, H., Mi, Z., Bowles, D.E., Bhattacharya, S.D. and Kuo, P.C.
Notes: The authors investigated the role of the ubiquitin E3 ligase PDLIM2 in the degradation of signal tranducer and activator of transcription 1 (STAT1). They showed that activation of PDLIM2 and subsequent STAT1 ubiquitination require protein kinase C-mediated phoshorylation of PDLIM2 on serine 137. Polyhistidine-tagged PDLIM2 and polyhistidine-tagged mutants PDLIM2-S137A and PDLIM2-S137D were purified using the MagneHis™ Protein Purification System for use in in vitro phosphorylation and ubiquitination assays. One mechanism used to assess levels of STAT1 was a reporter assay using RAW264.7 cells transfected with a pGL3-based construct containing a interferon γ-activated sequence (GAS) upstream of the firefly luciferase reporter gene. Expression of wildtype PDLIM2, but not the mutant forms, resulted in much lower levels of STAT1 protein, and thus lower luciferase activity, when cells were challenged with lipopolysaccharide. The pRL-TK Vector was used to normalize for transfection efficiency. Luciferase assays were performed using the Dual Luciferase® Reporter Assay System and Reporter Lysis Buffer. (4152)
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Dual-Luciferase® Reporter Assay System
MagneHis™ Protein Purification System
pRL-TK Vector
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Yun, C.S., Suzuki, C., Naito, K., Takeda, T., Takahashi, Y., Sai, F., Terabayashi, T., Miyakoshi, M., Shintani, M., Nishida, H., Yamane, H. and Nojiri, H.
Notes: The authors investigated the expression of genes encoding histone-like (H-NS) proteins from the self-transmissible pCAR1 plasmid and Pseudomonas putida KT2440 genome, as well as the interaction of H-NS family members in vitro. Gene expression was quantified using quantitative RT-PCR and RNA templates that were treated with RQ1 RNase-Free DNase to degrade contaminating DNA. Interactions between Pmr and other H-NS proteins were monitored using pull-down assays. His-tagged Pmr was expressed in E. coli, purified and used as bait for FLAG-tagged H-NS family proteins. Protein purification of His-tagged proteins was performed using the MagneHis™ Protein Purification System. (4119)
RQ1 RNase-Free DNase
Koschubs, T., Lorenzen, K., Baumli, S., Sandström, S., Heck, A.J. and Cramer, P.
Notes: The authors expressed the seven subunits comprising the middle module of Mediator, the 1.4MDa coactivator complex required for regulated transcription by RNA polymerase II, in E. coli. To detect and characterize the interaction of these subunits within the module, they coexpressed combinations of these subunits and performed pull-down assays. This enabled them to publish a subunit interaction map. Coexpression and pull-down assays were performed using proteins purified using MagneHis™ Ni-Particles. (4122)
MagneHis™ Ni-Particles
Yang, S.H. and Sharrocks, A.D.
Notes: The authors identified proteins that bind to small ubiquitin-like modification (SUMO) proteins using a yeast two-hybrid screen. The proteins ability to bind SUMO was confirmed using protein:protein interaction studies. In these studies, recombinant SUMO-1 was expressed with a His6 tag and immobilized using MagneHis™ Ni-Particles. Putative SUMO-binding proteins were expressed with a GST tag and the proteins were co-incubated to allow them to interact. Proteins bound to immobilized SUMO-1 were eluted with Laemmli sample buffer and detected by Western blot. (4121)
Mohl, D.A., Huddleston, M.J., Collingwood, T.S., Annan, R.S. and Deshaies, R.J.
Notes: The protein phosphatase Cdc14 is sequestered in the nucleolus during interphase and, after successful interphase, is dispersed from the nucleolus throughout the cell by an unknown mechanism to drive a cell's exit from mitosis. The authors determined that Cdc14 contains a nuclear localization signal (NLS), but phosphorylation of serine and threonine residues adjacent to the NLS interferes with localization. These phosphorylation sites were mapped using trypsin and AspN digestion, followed by mass spectrometry. The authors showed that phosphorylation of Cdc14 is mediated by the protein kinase Dbf2-Mob1 by co-incubating purified Dbf2-Mob1 and Cdc14 in the presence of γ-[32P]ATP. Dbf2-Mob1 was expressed with a His6 tag and purified using MagneHis™ Ni-Particles. (4120)
Sequencing Grade Modified Trypsin
Muik, M., Frischauf, I., Derler, I., Fahrner, M., Bergsmann, J., Eder, P., Schindl, R., Hesch, C., Polzinger, B., Fritsch, R., Kahr, H., Madl, J., Gruber, H., Groschner, K. and Romanin, C.
Notes: The authors performed protein pull-down assays to characterize the interaction of ORAI1 and STIM1, two protein components of the calcium-release calcium current. His6-STIM1 C terminus and ORAI1 were synthesized using the TNT® Coupled Reticulocyte Lysate System in the presence of 35S, and His6-STIM1 C terminus was immobilized using MagZ™ Binding Particles. An aliquot of the TNT® reaction expressing ORAI1 was added to the particles, and proteins were washed, eluted using increasing concentrations of imidazole (10–40mM) and analyzed by SDS-PAGE. In a second set of pull-down assays, His6-STIM1 C terminus was used to pull down ORA1 N- and C-terminal fragments expressed as GST fusion proteins. The His6-STIM1 C terminus protein was purified from transiently transfected HEK293 cells using the MagneHis™ Protein Purification System. (3781)
MagZ™ Protein Purification System
TnT® T7 Coupled Reticulocyte Lysate System
Cheung, Q.C., Turner, P.V., Song, C., Wu, D., Cai, H.Y., MacInnes, J.I. and Li, J.
Notes: One potential source of antibiotic-resistant bacteria is food-producing animals. The authors examined the ability of protegrin-1 (PG-1), an antimicrobial peptide, to protect wildtype and transgenic mice expressing PG-1 against bacterial infection. As part of the cloning strategy to produce the PG-1 expression construct, the authors amplified and cloned full-length PG-1 into the pGEM®-T Easy Vector. To test the bactericidal activity of PG-1 expressed in transgenic mice, radial diffusion assays were performed, in which test samples were added to a well containing E. coli and the clear antibacterial zone was measured. Two of the test samples were neutrophil secretions from the PG-1 transgenic mice and purified polyhistidine-tagged PG-1 protein, purified using the MagneHis™ Protein Purification System. (3896)
pGEM®-T Easy Vector System I
Hanington, P.C., Brennan, L.J., Belosevic, M. and Keddie, B.A.
Notes: The authors identify two partial transcripts that encode granulin-like molecules in Aedes albopictus and Manduca sexta. To test the hypothesis that granulin is a highly conserved growth factor that acts on insect cells, the authors expressed recombinant goldfish granulin with an N-terminal His6 tag, purified the recombinant protein, exposed A. albopictus Aa23 embryonic cells and M. sexta haemocytes to the purified protein, then monitored cell proliferation using a BrdU proliferation assay. Recombinant goldfish granulin was expressed in E. coli and purified using MagneHis™ Ni Particles. (3964)
Mie, M., Shimizu, S., Takahashi, F. and Kobatake, E.
Notes: The authors developed an in vitro selection system that is based on ribosome display and favors identification of 5´-untranslated regions (UTRs) with high translation efficiencies. A 5´-UTR random library was created in which the 5´-UTRs were upstream of a polyhistidine-tag/Renilla luciferase-coding region. In vitro transcripts from this library were translated in vitro using the Flexi® Rabbit Reticulocyte Lysate System. The authors preferentially selected mRNAs with high translational efficiencies by shortening the translation time and capturing ternary complexes of mRNA, ribosome and nascent proteins. These complexes were captured using MagneHis™ Ni Particles. RNA was extracted from these complexes and used as a template in RT-PCR for the next round of selection. Before and after each round of selection, 9µl of RNA was translated in vitro, and 20µl of translated product was removed every 5 minutes to measure Renilla luciferase activity and monitor translation efficiency. Renilla luciferase was measured using the Renilla Luciferase Assay System. After two rounds of selection, RT-PCR products were cloned into a pUC18 vector, the sequences of the resulting plasmids were confirmed, and 0.5µg of plasmid was translated in vitro using the TNT® T7 Coupled Rabbit Reticulocyte Lysate System to further evaluate translation efficiency. (3963)
Flexi® Rabbit Reticulocyte Lysate System
Renilla Luciferase Assay System
Schäfer, P., Cymerman, I.A., Bujnicki, J.M. and Meiss, G.
Notes: The authors used biochemical and mutational analysis to characterize human lysosomal DNaseIIα. Native DNaseIIα and site-directed mutants were expressed as Flag-His6-DNaseIIα and HA-tagged DNaseIIα using expression constructs created with the pCI Vector. Protein was expressed by transiently transfecting HEK 293-T cells using the TransFast™ Transfection Reagent. Flag-His6-DNaseIIα was purified using the MagneHis™ Ni-Particles, and this purified protein was used in nuclease assays to monitor catalytic activity and in gel filtration experiments and coimmunoprecipitation assays with HA-DNaseIIα to determine whether the active enzyme is monomeric. (3786)
pCI Mammalian Expression Vector
TransFast™ Transfection Reagent
Mi, Z., Oliver, T., Guo, H., Gao, C. and Kuo, P.C.
Notes: The authors investigated the role of short COOH-terminal osteopontin (SC-OPN), which is a product of thrombin cleavage of osteopontin, in tumor metastasis. The authors expressed SC-OPN as a fusion with a cyan-shifted variant of green fluorescent protein (SC-OPN-CFP)and cyclophilin C, a marker of metastatic function, as a fusion with the yellow-shifted variant (CyC-YFP). The fusion proteins were expressed with a His tag, and proteins were purified using the MagneHis™ Protein Purification System. Purified SC-OPN-CFP and CyC-YFP were incubated with 4T07 cells and the cells were analyzed by flow cytometry and confocal microscopy to determine whether the proteins bound to the CD147 cell surface glycoprotein. In addition full-length OPN, truncated forms of OPN, and osteopontin with a mutated thrombin site (Mu-OPN) were expressed as his-tagged proteins and purified from COS7 cells using the MagneHis™ Protein Purification System. These purified proteins were added to the mouse mammary tumor cell line 4T07, and cell migration and invasiveness were measured to determine the effect on metastatic activity. (3787)
Nakase, Y., Fukuda, K., Chikashige, Y., Tsutsumi, C., Morita, D., Kawamoto, S., Ohnuki, M., Hiraoka, Y. and Matsumoto, T.
Notes: This study sought to determine the roles of the tsc1+, tsc2+ and rhb1+ gene products in the starvation response in Schizosaccharomyces pombe. Recombinant Rhb1 was expressed as a polyhistidine-tagged protein in E. coli using the pET30a vector and used as the antigen for polyclonal antibody production. Recombinant Rhb1 was purified using the MagneHis™ Protein Purification System. (3568)
Hanington, P.C., Barreda, D.R. and Belosevic, M.
Notes: These authors expressed a recombinant form of a novel hemapoietic granulin from goldfish. This recombinant granulin was expressed with a His6 tag in a 1-liter culture of BL21 Star™ (DE3) cells and purified from the culture supernatant using the MagneHis™ Protein Purification System. The purified protein was used to immunize rabbits and produce an affinity-purified rabbit anti-goldfish granulin IgG for immunodetection. The purified protein was also added to goldfish primary kidney macrophage cultures to determine if granulin stimulates macrophage proliferation. (3567)
Lin, C.T., Moore, P.A., Auberry, D.L., Landorf, E.V., Peppler, T., Victry, K.D., Collart, F.R. and Kery, V.
Notes: The authors compared two methods to purify a subset of 236 hypothetical hexahistidine-tagged Shewanella oneidensis proteins: a large-scale, lower-throughput filtration separation protocol using Ni-NTA Superflow columns (Qiagen) and a lower-scale but higher-throughput magnetic bead-based purification protocol using MagneHis™ Ni-Particles. They examined several factors that can affect yield and efficiency of protein binding to these two matrices, including steric factors, protein size, amount of cell lysate and cell lysis protocol. They concluded that both matrices seem to have similar protein-binding capacities, and the larger-scale and lower-scale methods resulted in 8.7µg/OD600 and 8.8µg/OD600, respectively. When examining native proteins of similar sizes, they found binding differences between monomeric and oligomeric forms, most likely due to steric hindrances around the polyhistidine tag. However, they found no correlation between protein yield and protein size (as measured in kDa or as Stokes radius). The authors calculated that the maximum yield was approximately 200µg of protein from a lysate load of 30 OD600 and observed that some proteins are difficult to elute from the MagneHis™ Ni-Particles. Inefficient elution can affect yield. About 30% of proteins were purified to >90% homogeneity and about 40% to >80% homogeneity using MagneHis™ Ni-Particles. Fourteen of the proteins were not expressed in sufficient quantity for protein purification. Finally, the authors concluded that the automated filtration process does not save much time or labor compared to the manual filtration process, and the lower-scale MagneHis™ protocol is efficient with minimal error rate. The authors used the Biomek® FX automated workstation to process 96-well plates of E. coli cultures expressing the various S. oneidensis proteins. (3848)
Muranaka, N., Hohsaka, T. and Sisido, M.
Notes: The authors devised an mRNA display system to generate a peptide library with multiple nonnatural amino acids incorporated into the proteins, an important feature of peptide libraries for successful drug discovery. An mRNA with 3 four-base codons at a random position was used as a template in an in vitro translation system in the presence of charged tRNAs carrying four-base codons. In vitro translations were performed using 3.6 × 1013 molecules of mRNA template and the E. coli S30 Extract System. The mRNA template contained a T7 tag sequence, so the translation products could be detected using an anti-T7 tag antibody and the Anti-Mouse IgG (H+L), AP Conjugate. The mRNA-displayed peptides also incorporated a polyhistidine tag so that they could be purified using the MagneHis™ Ni-Particles. After selecting for the desired protein characteristic, the mRNA portion of the mRNA-displayed peptides was reverse transcribed and quantitated by real-time PCR. PCR products were cloned into the pGEM®-T Vector prior to sequencing. (3651)
pGEM®-T Vector System I
pGEM®-T Vector System II
Anti-Mouse IgG (H+L), AP Conjugate
E. coli S30 Extract System for Linear Templates
Mayor, T., Lipford, J.R., Graumann, J., Smith, G.T. and Deshaies, R.J.
Notes: These authors used a two-step process to purify a spectrum of ubiquitylated proteins from yeast expressing polyhistidine-tagged ubiquitin. First, two polyubiquitin-binding proteins were fused to glutathione-S-transferase purification tags (GST-Rad23 and GST-Dsk2), expressed in bacteria and purified by glutathione-Sepharose resin. A cleared yeast lysate was added to the GST-coupled proteins, mixed and washed. Elution of bound proteins was performed using a urea buffer (8M urea, 100mM NaH2PO4, 10mM Tris-HCl, pH 8.0). The eluate was then mixed with 125µl MagneHis™ Ni-Particles previously washed in the urea buffer. After incubation, the MagneHis™ particles were stringently rinsed with urea buffer + 0.5% Triton® X-100. To generate peptides for MS-based sequencing, proteolytic digests were performed directly on the beads. The enzymes used were endoproteinase Lys-C (incubated for 5 hours) and trypsin (incubated for 16 hours). After digestion, the protein sample was analyzed by mass spectrometry (ESI-MS). (3286)
Delroisse, J.M., Dannau, M., Gilsoul, J.J., El Mejdoub, T., Destain, J., Portetelle, D., Thonart, P., Haubruge, E., and Vandenbol, M.
Notes: In this study, the researchers used the MagneHis™ Protein Purification System to purify recombinant, histidine-tagged Tribolium castaneum (red grain beetle) esterase from Pichia pastoris. The T. castaneum esterase gene, termed TCE, was cloned into pGAPZα A, pGAPZ B, and pPICZ B vectors and P. pastoris cultures transformed with each vector were analyzed for esterase activity. TCE yields varying from 7-80mg/L were obtained from P. pastoris cultures containing the above constructs using the MagneHis™ Protein Purification System. Specific activities of histidine-tagged TCE ranged from 4.5 to 5.7 U/mg. (3297)
Layer, G., Grage, K., Teschner, T., Schünemann, V., Breckau, D., Masoumi, A., Jahn, M., Heathcote, P., Trautwein, A.X. and Jahn, D.
Notes: These authors studied the activity of oxygen-independent coproporphyrinogen III oxidase HemN, an enzyme involved in converting coproporphyrinogen III to protoporphyrinogen IX in heme and chlorophyll biosynthesis. The activity assay for HemN requires protoporphyrinogen IX oxidase to convert the end products of the HemN reaction to a detectable form. Recombinant protoporphyrinogen IX oxidase was expressed as a polyhistidine-tagged protein in E. coli strain BL21-Codon-Plus(DE3)-RIL and purified using the MagneHis™ Protein Purification System. (3569)
Markillie, L.M., Lin, C.T., Adkins, J.N., Auberry, D.L., Hill, E.A., Hooker, B.S., Moore, P.A., Moore, R.J., Shi, L., Wiley, H.S., and Kery, V.
Notes: Researchers compared MagneHis™ Ni-Particles to other vendors' his tag protein purification systems in a model co-precipitation system with various bait proteins and a Shewanella oneidensis degradosome. Peptides from the S. oneidensis degradosome that co-purified with the bait proteins were analyzed by SEQUEST analysis. Bait proteins included E. coli polynucleotide phosphorylase, (PNP), RNase E and the RNA helicase. The authors describe the use of the MagneHis™ Ni-Particles in a simple and efficient system that can be automated for screening purposes. The authors also discussed optimizing the elution conditions, amount of bait protein and wash steps. (3280)
Babb, K., von Lackum, K., Wattier, R.L., Riley, S.P. and Stevenson, B.
Notes: These authors characterized metabolic pathways in Borrelia burgdorferi, the causative agent of Lyme disease, focusing on the 5´-methylthioadenosine/S-adenosylhomocysteine nucleosidase (Pfs) and the autoinducer-2 production protein LuxS. Recombinant LuxS and Pfs proteins from both B. burgdorferi and E. coli were expressed as polyhistidine-tagged proteins in BL(21)DE3pLysE and purified using the MagneHis™ Protein Purification System. The purified proteins were then used in enzyme activity assays. The E. coli LuxS and Pfs proteins were used as positive controls for enzyme activity. (3566)
Katayama, T., Sakuma, A., Kimura, T., Makimura, Y., Hiratake, J., Sakata, K., Yamanoi, T., Kumagai, H. and Yamamoto, K.
Notes: In this study, the fucosidase domain of the Bifidobacterium bifidum 1,2-alpha-L-fucosidase was purified as a carboxy-terminal fusion to hexahistadine. The fucosidase domain was cloned into an inducible T7 expression vector and transformed into the bacterial strain BL21(DE3). The expressed protein was then purified using the MagneHis Protein Purification System. The protein was eluted with a gradient of 0-1M NaCl in 10mM sodium phosphate buffer (pH 6.5). (3170)
Lee, M.H., Osaki, T., Lee, J.Y., Baek, M.J., Zhang, R., Park, J.W., Kawabata, S.I., Soderhall, K., and Lee, B.L.
Notes: Researchers used MagneHis™ Ni-Particles to purify his-tagged peptidoglycan recognition protein-1 (PGRP1 and PGRP2) that had been excreted into medium supernatants. The his-tagged proteins were created by making fusion-protein expression vectors from isolated H. diomphalia larvae cDNA and the pMT/Bip/V5-His vector (Invitrogen). The construct was then stably transfected into Drosophila Schneider S2 cells, and the medium was monitored for secreted protein by Western blot analysis. (2837)
Jung, Y. and Lippard, S.J.
Notes: MagneHis™ Ni-Particles were used to enrich for histidine-tagged T7 RNA Polymerase DNA bound to templates with T7 promoters. The templates contained various platinum adducts that stalled or stopped in vitro transcription reactions with T7 polymerase. (2818)


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Shoe Thrown at Hillary Clinton During Vegas Speech
A woman has been arrested by police in Las Vegas after she threw a shoe at former Secretary of State Hillary Clinton Thursday during a speaking event.
Hillary Clinton narrowly missed being pelted by a shoe tossed at her during a speech in Las Vegas to a recycling industry group.
A tape of the incident aired by KTNV shows an object quickly whizzing by Mrs. Clinton's head. "Was that a bat?" she asked, before joking: "Is that somebody throwing something at me? Is that part of Cirque de Soleil?," adding: "My goodness I didn't know solid waste management was so controversial."
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The video also shows a woman being quickly whisked away by security. The speech was to the Institute of Scrap Recycling Industries meeting being held at the Mandalay Bay resort.
Ilene Rosen, the wife of a conventioneer from Denver who was seated in the second row, said the woman had walked down the aisle to within six rows of the front of the seating area, threw several items, turned around, put her hands in the air and walked toward the back of the room. Security officers quickly caught up with her.
Brian Spellacy, U.S. Secret Service supervisory special agent in Las Vegas, said the woman who allegedly threw the shoe was being questioned and would face criminal charges. Mr. Spellacy declined to identify the woman, and he said it wasn't immediately clear what the charges would be.
Mr. Spellacy and Mark Carpenter, spokesman for the recycling institute, said the woman wasn't a credentialed convention member and wasn't supposed to have been in the ballroom.
A black and orange shoe was recovered from the stage, Mr. Spellacy said.
An attendee later handed a reporter a piece of paper that was apparently also thrown by the woman. It appeared to be a copy of a Department of Defense document labeled confidential and dated August 1967; it referred to an operation "Cynthia" in Bolivia. There was a Bolivian army operation of that name in 1967 to capture revolutionary Che Guevara.
The incident recalled a similar incident in 2008, when President George W. Bush dodged shoes thrown at him by an Iraqi journalist, as seen in the clip below.
– The Associated Press


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Rehab: Summer Camp for the Broken
I wake up in a twin bed. My mouth is dry and my institution-issued cotton pants are vacuum wrapped to my legs with sweat. The wall clock, illuminated from the light bleeding in from the hallway, reads 3:14am. I'm withdrawing, scared, tired, hopeless. I look toward the opposing end of the room and see my roommate Jeff.
Jeff is a homeless man in his mid 50's, fat, bearded, and bedecked in his institutional scrubs, sitting up in his twin-sized bed holding a handful of mayonnaise packets, emptying them into his mouth one by one. He looks like Santa Claus would look if left to his own mind in solitary confinement for 25 years: insane, broken, murderous, jolly.
But I guess he's technically not homeless—he is living here, in rehab. At least until his government assistance runs out. Then again, I'm in rehab too and I have nowhere to go when I get out of here. I guess I'm homeless too. God...I don't even like mayonnaise. I'll be lost....
* * *
Rehab is essentially summer camp for the broken. Your family has to drag you there kicking and screaming, you cry the first night, everyone hates the counselors, a few people get kicked out early, and you meet someone you think you will love forever. Oh yeah, also, arts and crafts.
Dairy condiments aside, I would go back in an instant. Miami might have beautiful women, and Rome may have the Colosseum, but the Malvern Institute has what no geography can offer: 50 people on the brink of self-annihilation. Say what you want about the good or bad of it, I'm just saying as far as unique life experiences go, there is nothing richer.
"Wheat bread? Really? It's a wonder you know anything about bread at all."It's interesting how these personalities would remove themselves from what was actually happening. I would hear things in the dining room like, "This white bread is disgusting and I'm not eating this garbage. This is all empty calories!" Really? Because two weeks ago you were blowing a fat Serbian guy in the backseat of an El Dorado for meth. But the grilled cheese in rehab—you know, the place you're at because you couldn't stop blowing fat Serbians for meth?—isn't made with wheat bread? Yes, that is the precipice you refuse to cross in regard to health, the fucking Wonder Bread. You shot at a cop car and landed in rehab on a technicality but the fact that this place doesn't have a vegan menu, well that is just unacceptable.
Regardless of what your notion is of people who have been in rehab, there is one truth that is undeniable: they are the most interesting people you know. They are the ones you talk about, the ones who crossed the line, the ones with stories that end in, "...so I woke up on the roof of my old high school and it took me a week to wash all of the blood and semen out of my hair." They are the ones eating mayonnaise packets because they find comfort in doing something they have done for years in order to subsist on the street, an old survival tactic passed down from one addict to another. They are the ones who survived hell and are trying to crawl out.
The odds of abusing a substance again after leaving rehab is astronomical. The success rate of staying clean for one year is about 2%. I haven't had a drink in over two years and I still hate mayonnaise.
Congratulations...here's to the next day Bill...and the next...and the next...rinse repeat. ;)
You are a true gentleman but I don't bathe.
Congrats and I think I love you.
I love you too...always and forever.
This wasn't nearly long enough. I am riveted. I can't wait to have an apple juice with you. And I used to like mayonnaise. Now I prefer mustard. Or ketchup. Ketchup is good too. It goes with everything. Except cereal. Maybe Cheerios. I think it goes with Cheerios.
I have always and forever hated mayonnaise. Awful stuff. Basically just lard and... well, lard. Kudos on another disturbingly wonderful piece.
Powerful stuff. It's good to hear that you're doing well. Keep adding up the days and proving that probability has nothing to do with it.
You are my hero. Always have been and always will be.


text: Hotel in Nantucket, MA Nantucket Hotels - As one of the oldest hotels on Nantucket, Jared Coffin House embodies the true essence of the former whaling town, offering charming accommodations and a variety of modern day comforts. Originally built as a family residence by Jared Coffin, one of the most successful ship owners of his time, the three-story brick mansion was the first of its kind on the island. In 1846, The Great Fire destroyed over two hundred buildings in the Nantucket community. While a third of the community burned to the ground, Jared Coffin House withstood the flames. Today, Jared Coffin House stands as one of the oldest New England hotels, giving visitors a taste of Nantucket history and classic New England style that so many travelers know and love. It consists of the Main Building and the Daniel Webster, located next door. From October 20- April 8, 2016 the Main Building will be closed with guest rooms avaialble in the Daniel Webster located next door. Nantucket Island Vacation Destination Jared Coffin House provides convenient access to shopping, beaches, sailing, fishing, kayaking, surfing, restaurants, museums, historic lighthouses, and more. As a centerpiece of the Nantucket Historic District, the hotel is located in the midst of outstanding restaurants, boutiques and galleries. Massachusettes Getaway Specials There's no better way to enjoy the classic comforts of Jared Coffin House. Treat that special someone to a romantic sunset cruise or island tour. Pamper yourself at our sister hotel's spa, White Elephant Spa. Book a romantic getaway or spa package today. Meetings & Events Host an intimate board meeting or employee outing within the brick walls of our historic Nantucket hotel. Your distinguished guests and colleagues will find themselves relaxed and at ease in our historic, two-building property. For larger meetings and events, our sister hotel, the White Elephant, is the perfect venue for everything from weddings to social gatherings and business meetings. Nantucket Island Accommodations The Jared Coffin House features charming rooms that combine unique, antique-style furnishings with modern features, such as flat-screen HDTVs, air-conditioning, and free Wi-Fi. Guests enjoy beach towels/chairs, bottled water and comfy robes and slippers. Services & Amenities Enjoy the classic elegance of a historic home-turned-hotel in the heart of Nantucket. We offer personalized service at every turn and a full range of amenities to ensure your utmost comfort.


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The Eagle County Regional Airport (EGE) 20-year Master Plan is in the final stretch.
County commissioners met with EGE representatives and Jviation consultants on Tuesday for an update on the plan. Jviation is a Denver-based firm hired in March 2011 to direct the master plan process.
The plan will ensure that development at the airport is consistent with the goals of the county and the community. It's being used to determine what facilities are needed, when they will be needed and how much they will cost.
EGE Director Greg Phillips said there are basically three parts left to complete before submitting the document for Federal Aviation Administration approval this spring.
"We're thinking we'll have a capital improvement plan and a financial strategy (to pay for the improvements) by March," he said.
The third step is drafting an airport layout plan that maps the property and improvements in detail.
Jviation plans to hold another open house for public input this spring.
Phillips reminded people that the improvements identified in the master plan will only be developed on an as-needed basis, with individual review and county approval for each project.
"Operations (take-offs and landings) are expected to increase 11 percent over 20 years," said Dave Nafie, planning manager for Jviation. "Meeting the level of demand will require expansion."
Enplanements (number of passengers boarding planes) are forecast to increase by 39 percent in 20 years and the number of private planes based at EGE are expected to rise by 41 percent.
"This is a highly seasonal airport and most traffic occurs in very concentrated periods," Nafie said.
Some of the key improvements include expanding the runway, taxiways and terminal. Phillips said EGE will likely need a longer runway if it attracts international flights.
"There is also some question about what kind of aircraft we'll be seeing in the future, as the Boeing 757 approaches retirement and new aircraft enter the airline fleet in coming years," he said.
Expanding the terminal is another key improvement that's on the table.
"It would be cheaper to simply expand outward (on the ground level) but we are already limited by our aircraft ramp depth and pushing the terminal out would push aircraft parking out to the point where they are unacceptably close to the runway. That could result in losing space to park the bigger planes at the gates and could, in the worst case, force us to move the runway," Phillips said. "The best option is to add space is to add a second level to the terminal holding room area, with room on either end to add gates as well."
The second level would likely be built within 10 years. In the course of the 20-year master plan, other identified terminal expansions will remain at ground level and cost about $27 million. The second-level expansion is anticipated to cost another $22.5 million.
The biggest expansion the terminal might see in the second phase is a second axis - an entrance that branches off to the south from the current building. Phillips said that would allow better access to more people and would also separate arriving and departing vehicle traffic on different roadways.
That would also affect parking, however. Parking is currently free at EGE.
"At some point, given the cost and need of these future improvements, it will be important to consider all revenue sources available to the airport. That includes the potential for paid parking," Phillips said.
Phillips said the rental car agencies might have to be moved off-site at some point, though that is not shown in the current master plan and likely would not be considered until sometime beyond the next 20 years.
"For now we want the rental cars at the terminal because convenience is our No. 1 selling point," he said.
The access road and parking improvements are projected to cost $24.5 million.
The commissioners recently ranked the priority of projects, with airfield safety, terminal improvements and roadways identified as most important. These rankings were also aligned with the priorities identified by the EGE Planning Advisory Committee, which met previously on Monday to preview the plan.
Phillips said there are basically five categories of funding options to cover the projects. First, there are the FAA entitlement and discretionary funds. (Entitlement funds are based on the number of enplanements at the airport and discretionary funds are doled out to projects that are shown to be high enough priorities.) The Colorado Department of Transportation also has a funding program for airports. Then there are local funds and bonds, with the possibility of some local or statewide grants to cover the projects, Phillips said.
Something that might sway a favorable relationship with the FAA is the fact that EGE is one of the first airports in the country to participate in a "planning for compliance" program.
"Our aim in this process is to show the FAA we're transparent and fully intent on complying with requirements by opening all our closets and drawers for them to see inside," Phillips said.
The FAA has many conditions and standards attached to its funding but it isn't able to dedicate its staff to actively see if airports are fully complying. The program is intended to have airports be proactive with the standards. If an airport is found out of compliance, it can be made ineligible for grants until it comes into compliance.
Phillips said a potential down side of participating in the program is that EGE might discover something out of compliance that requires corrective action. That hasn't been the case so far, though, and EGE's cooperation might help in "intangible ways," as one of the pilot program airports willing to participate, he said.
The commissioners are hoping to sign off on the final master plan in May, once it has been reviewed by the FAA, but Phillips warned that the FAA might take six months or longer to conduct its final review of the plan.


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$100 margarita! America's most expensive cocktails
The St. John cocktail from Osteria 177 in Annapolis, Maryland, mixes Louis XIII cognac, Grand Marnier Cuvee du Centenaire, Meyer lemon juice, syrup made from Meyer lemon and Grade 1 saffron. Don't forge the garnish — 23k edible gold flakes. (Photo Credit: Osteria 177) by Kristin Hunt (CNN) — Think the $18 martini you ordered at the hotel bar was steep? That was peanuts. As in, the little dish of complimentary germ-infested peanuts on the bar. From California to New York and everywhere in between, ritzy bars are playing a crazy game of "Who Can Make the Costliest Cocktail?" — and the competition is as stiff as the product. We scoured the country for examples of the most over-the-top pours we could find, excluding those accompanied by crown jewels or crystal goblets. Even still, you may need to skip a mortgage payment or two to enjoy a night of these extravagant cocktails.


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Domestic abuse is abuse, whether physical or psychological. The thriller "Angel Street," which will be performed by the Mid-Ohio Valley Players, tackles the topic of the ongoing psychological and eventual physical abuse of a woman at the hands of her murderous husband.
Set in Victorian London, the play, also known as "Gas Light" in Britain and later an American movie starring Ingrid Bergman and Charles Boyer, focuses on the Manninghams. Jack and Bella are a middle class married couple who seem to have some serious issues in their marriage, including the husband's violent and shady past and his efforts to drive his wife mad by psychologically torturing her with "kindness" - making her think that he is concerned for her well-being.
"Jack is a husband who is making his wife believe she is going crazy," said Ray Ryan, a veteran performer on the MOVP stage, who is portraying Jack. "He really is a bad guy. He starts off being verbally abusive and it progresses to some physical violence towards the end."
Article Photos
Photo submitted by Bob Parsons
Ray Ryan as Jack Manningham, left, Tara Polan as his wife, Bella, center, and Olivia Polan as the maid, right, rehearse a scene from the Mid-Ohio Valley Players production of "Angel Street." The thriller deals with psychological domestic abuse and a portion of money raised from a 50/50 drawing, to be held each night, will benefit EVE, Inc.
Ryan said he grew a beard to play the character, something which was noted in the character description in the script, and that costuming really helps him to get into a role. However, Ryan wouldn't describe himself as a method actor.
"I have not taken the role home with me. I am able to leave the character once I leave the stage," he said.
Tara Polan plays the tragic wife Bella; Kathy Biery plays the housekeeper; and Olivia Polan is the maid, and object of Jack's flirtatious advances. Marty Durham is Inspector Rough, who comes to Bella's aid.
Fact Box
If you go
What: "Angel Street," a production by Mid-Ohio Valley Players. A 50/50 drawing will be held each night with proceeds going to EVE, Inc.
When: Friday and Saturday and March 23-24, 30-31 at 8 p.m.; March 25 at 3 p.m.
Where: Players' theater at 227 Putnam St. in Marietta.
Tickets: Adults, $10, senior/student $8, youth $5. Tickets available at midohiovalleyplayers.org., at the theater box office and at Marty's Print Shop, 307 Third St.
For information: midohiovalleyplayers.org.
EVE, Inc. hotline: 374-5819.
"I really liked the idea of doing a mystery/drama," said Durham, 50, of Belpre, who has been with the MOVP for nine years. "My character adds a little bit of tension relief. He's eccentric and he thinks out loud."
Mark Abicht and Kermit Polan round out the cast of characters that are being directed by current MOVP president and long-time member, Billie Harmon.
The production will be working in conjunction with EVE, Inc., the local domestic violence shelter, to bring awareness and raise money for the organization.
"There will be a 50/50 drawing each night and we will get the proceeds," said EVE co-executive director, Annelle Edwards. "We will also have a table set up to provide brochures, how to donate to EVE and more information."
Edwards, who previewed the play Monday evening, said the issues in this play may strike a chord with those who have suffered abuse of any kind.
"(The play) is kind of intense and might churn up some emotion. Domestic abuse perpetrators do often use psychological abuse but many (sufferers) may not think that they are being abused since it is not violent. And it is very hard to prove something like that to the police," Edwards explained.
The show will be performed Friday and Saturday, as well as March 23, 24, 30 and 31 at 8 p.m. and March 25 at 3 p.m. Tickets are available at midohiovalleyplayers.org.


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Science Features
Cell phones, electric cars and lasers require a few essential, but rare, elements that have been produced and marketed mostly by China. Now, new USGS research on timeworn samples from the U.S. western mining era show potential new sources of the fundamental ingredients used in 21stcentury technologies.
USGS scientists are reanalyzing rocks, minerals and associated mine tailing samples – some collected 120 years ago – to assess their rare and critical elements, which could become potential for economic development.
Rare earth elements (or metals) are a set of 17 chemically-related elements, and are specifically made up of the fifteen lanthanides plus scandium and yttrium. Scandium and yttrium are considered rare earth elements since they tend to occur with the lanthanides in nature and exhibit similar chemical properties.
Despite their name, rare earth elements (with the exception of the radioactive promethium) are relatively plentiful in the Earth's crust, with cerium being the 25th most abundant element at 68 parts per million (similar to copper). However, because of their geochemical properties, rare earth elements are typically dispersed and not often found concentrated in economically exploitable ore deposits.
In addition to studying the nature and occurrence of rare earth elements, the USGS is conducting research on the distribution, occurrence and understanding of a range of new technology critical (strategic) minerals such as indium, tellurium, gallium and all others. These rare metals are of increasing interest due to their usage in many new materials such as LCD screen, solar cells and medical imaging devices.
"Without rare earths we'd be back to having black-and-white cellphones again," said Ian Ridley, director of the USGS Central Mineral and Environmental Resources Science Center in Denver, Colo.
Laser Ablation Technology Helps Tap into Old Samples
Laser ablation is one method USGS scientists are using to discover pockets of unearthed and untapped critical mineral and rare earth resources.
"I get to shoot rocks with lasers to discover new (or old) information about rare metals contained within the rock," said Alan Koenig, the USGS scientist in charge of the tailings project. "We can tease out from the rock not only what it's made of, but we can discover a story about how it formed."
Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is the technique that is allowing USGS scientists to quickly analyze old (and new) rocks for the wide range of chemistry of interest to many aspects of USGS projects and society. The technique utilizes a high energy laser to "zap" a small hole in the sample and introduce it into the mass spectrometer (the ICP-MS part of the system). In doing so, the sample in virtually any form (mineral, powder, or even frozen liquids) is directly analyzed without the need for complicated, time-consuming or dangerous acid dissolving of the material. While the systems are still expensive to acquire, and commonly require highly-trained staff to develop and operate, LA-ICP-MS is becoming a common tool in geology, forensic and materials facilities.
Laser Ablation Lab
The USGS has been a world leader in the development of methods related to the analyses of geologic and earth related materials. Laser ablation ICP-MS is no exception. The USGS LA-ICP-MS Facility located in Denver, Colo., is one of the premier facilities for a wide range of applications and methods development. The USGS LA-ICP-MS facility is one lab where scientists are working on discovering and comprehending untapped resources, and new contributions to natural resources in the United States.
Links to USGS programs/publications that feature Rare Earths
Rare Earth Element Deposits: Interactive Map
USGS Fact Sheet 087-02 – Rare Earth Elements – Critical Resources for High Technology
USGS Scientific Investigations Report 2010-5220: The Principal Rare Earth Elements Deposits of the United States—A Summary of Domestic Deposits and a Global Perspective
USGS Scientific Investigations Report 2011-5094: Rare Earth Elements—End Use and Recyclability
USGS News Release: Research on Rare Earth Elements in Alaska Could Help Make Them Less Rare
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The Lumber Guy cut down a field of older sprinters in the stretch and held off a fast-closing Caixa Eletronica to win the Grade 1, $400,000 Vosburgh Invitational on Saturday afternoon at Belmont Park and earn a trip to the Breeders' Cup.
One of two 3-year-olds among the nine Vosburgh starters, The Lumber Guy was off alertly and settled in just behind pacesetters Poseidon's Warrior and Little Drama as they barreled through opening fractions of 22.57 and 45.06 seconds. Launching his bid approaching the quarter pole, The Lumber Guy grabbed the lead with a sixteenth of a mile to go and hit the wire 1 ¼ lengths clear of Caixa Eletronica.
"It was pretty simple," said Hall of Fame jockey John Velazquez. "Mike [Hushion] told me, 'He's quick, make sure you get him out of there running, and if he's taking you, leave him alone.' He thought he was going to finish for me, and he did."
The Lumber Guy's winning time was 1:09.22 in the "Win and You're In" race for the November 3 Breeders' Cup Sprint at Santa Anita Park, although owner Barry Schwartz indicated the Dirt Mile was also an option.
"This is always a good time of year for 3-year-olds," said Hushion. "They've gone through that maturing. I like 3-year-olds vs. older horses this time of year. I've been telling Barry for the past five weeks that he's been working like the real deal. We know he's got a good mile in him from the Jerome. Time will tell. I don't think we'll be doing any jumping to a mile and an eighth from here."
The Lumber Guy, who was making his first start since finishing sixth in the Grade 2 Peter Pan on May 12, returned $6.20 as part of the favored 2-1 entry with 6-year-old Sean Avery, winner of the 2011 Alfred G. Vanderbilt at Saratoga Race Course.
Bred in New York by Schwartz, the gray Grand Slam colt has four wins in six career starts, all this year, including the Grade 2 Jerome in April at Aqueduct Racetrack. With the winner' purse of $240,000, The Lumber Guy more than doubled his earnings to $465,800.
"We really felt going in that we were going to win today," said Schwartz. "I just joked, 'The only bad thing is that we're not going to get 10, 12-1 on him.' He had a month off on my farm after the Peter Pan because he was knocked out. Mike really took it easy getting him back, and he's just come back better than ever."
Caixa Eletronica, at 7 the oldest horse in the race, was 1 ¾ lengths clear of third-place finisher Poseidon's Warrior.
"I love the horse," said Javier Castellano, aboard the runner-up. "He's always honest, always tries hard. He's a pro. Every time he puts in a great effort."
Completing the order of finish were Little Drama, Sean Avery, Justin Phillip, Fort Loudon, Zero Rate Policy, and Rothko. Little Currier was scratched at the gate.
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Mr Jenkins wrote: He said, she later called him to apologise and that she had been told by her seniors from on high to keep asking about the personal alligations and not mention any wikileak revelations or lose her job.
IAmNiki wrote: Some say it makes him look more like a criminal.
crash wrote: sorry, i was assuming the people who read this forum were smart enough to to make the distinction between apparent creepiness of assange's personal life and the validity of wikileaks' mission.
edit: your argument might have some weight if i was a news anchor. any news organization that is spending time reporting on the legal proceedings while ignoring the substance of the leaks is participating in the smear campaign. but this isn't a news network. it's a forum where people discuss current events, hip hop, and youtube videos. there's a whole thread dedicated to a line in a lil wayne track for christ's sake.
Sage Francis wrote: That may be true, but no one needs to threaten our jobs in order for us to keep talking about his alleged personal indiscretions.
"And again, I ask, what's that got to do with the price of rice in China?"
Sage Francis wrote:
I'm not saying the charges are false. However, in the grand scheme of things, I'm saying that they don't matter. Does that mean that I'm a rape apologist? I don't think so, but I do see people being called that when they try to look beyond the allegations. Unless it has something to do with the information he has obtained and released to the public about our 'leaders', his personal life is of no concern to me.
Dan Shay wrote: The great thing about whisper campaigns is you don't have to be some muckety muck news anchor to spread them. We're in the day and age of the blogosphere.
Quote: AMY GOODMAN: The case against Assange has sparked international controversy, as well as controversy within the feminist community. We're joined by two women right now. Jaclyn Friedman is executive director of Women, Action, & the Media and the editor of the anthology Yes Means Yes: Visions of Female Sexual Power and a World Without Rape. She's joining us from Boston. Naomi Wolf is a social critic, author of seven books, including The Beauty Myth, The End of America. She's joining us here in New York.
We welcome you both to Democracy Now! Jaclyn Friedman, as this information comes out, why don't you talk about your thoughts on the—we can't even say charges against Julian Assange, because he has not yet been charged.
JACLYN FRIEDMAN: We can say allegations. Certainly these women are alleging a crime.
What I want to say is that these are—the details, certainly, have become more clear since that unauthorized leak, but we've known those basic facts for weeks, that the allegations—in fact, we've known them since August, that the allegations were that he held one woman down, that he raped another in her sleep. These allegations have been out there. The Guardian has been reporting them.
Rape is a very serious crime, and it's also one of the most underreported crimes across the globe. And one of the reasons is because every time the issue comes up in the media, people come out of the woodwork to blame the victims and to minimize the crime. And unfortunately, when we see someone who is a progressive hero, like Assange is, those critics, those people who are doing that minimization and that victim blaming often come from the left, as well as the right. And we've seen that across the board. Unfortunately, with—Naomi Wolf has participated in that, as well as Michael Moore, Keith Olbermann, Glenn Beck—of course, plenty of people on the right are participating, as well.
And the result of that is not only that these women are receiving death threats, they are in hiding—one of them has gone to Palestine, because she couldn't feel safe in Sweden anymore—but the even more important result of that, when we perpetuate rape myths in the media—and this is not just my opinion, this has been documented by social research—is that victims, nameless victims, victims who have been harmed by people who are not famous, become much more reluctant to take their experiences seriously, to report those experiences. The system, the justice system that's supposed to work for those victims—the cops, the juries, the prosecutors, the judges—they become much more reluctant to take these allegations seriously when they are reported. And men become less likely to identify their own behavior as sexually violent. And the result of all of that is that rapists go free.
And what we know about that is, the majority of rapists are repeat rapists. So, the result of perpetuating these rape myths in the mass media is that we literally are creating more rape in the world. And that's my main concern about the way these allegations have been discussed so far, is that it's doing real harm to real women around the world who have nothing to do with this case.
AMY GOODMAN: Naomi Wolf, your response?
NAOMI WOLF: Thank you. Well, Jaclyn, let me say that I'm very, very offended that you're suggesting that I'm blaming the victim. In fact, it's because of my 23 years of supporting rape victims, working in rape crisis centers, traveling around the world, to report more than any journalist I know, which, in a way, I've been very blessed to have had the chance to do so, from Sierra Leone to Bosnia to Ireland to the United Kingdom, interviewing people who support rape victims and work with the legal system—it's because of that that I'm raising my voice about these very ambiguous and corrupt allegations.
First of all, let me just correct you. And Jaclyn, these—The Guardian account, which is based on leaked original documents, doesn't say that he had sex with either of these women without the consent. The reason I'm hearing from rape victims across the world who are emailing me, saying, "I'm a rape victim. Thank you for standing up to put these charges in context," is that this is the only case I've ever seen in 23 years of supporting rape victims which is based on multiple instances of consent.
If you read these allegations, he took off Miss A's clothes too quickly for her comfort. She tried to tell him to slow down, but then, quote, "she allowed him to undress her." This is what the report says. The second woman says she woke to find him having sex with her. When she asked whether he was wearing a condom, he said no. Quote, "According to her statement, she said: 'You better not have HIV.'" He answered, "Of course not." Quote, "She couldn't be bothered to tell him one more time because she had been going on about the condom all night. She had never had unprotected sex before."
So, if you're going to treat women as moral adults and if you're going to take the issue of rape seriously, the person who's engaging in what he thinks is consensual sex has to be told, "I don't want this." And again and again and again, these women did not say, "This is not consensual." Assange was shocked when these were brought up as complaints, because he had no idea that this was not a consensual situation. Miss A kept Assange in her home for the next four days and threw a party for him.
So, because I take rape seriously, because I'm aware that in 23 years, you know, in Sweden, which has been criticized by Amnesty International for disregarding rape, for letting rapists go free, because you have a better chance in Sweden, if you're a rape victim, of, you know, dying in an accident or getting breast cancer than having a serious rape allegation prosecuted or getting any kind of legal hearing, according to Amnesty International's report "Case Closed"—it's because of that that I know that these charges are utterly, utterly atypically handled. In 23 years, I've never seen any man in any situation this ambiguous, involving this much consent, have any kind of legal process whatsoever. And all over the world, women who have been gang-raped, brutally raped, raped in alleyways, pimped, prostituted, trafficked, you know, their rapists go free.
So, yes, this stinks to me. And yes, it's about politics, and it's about the same kind of politics that dragged you, when you were trying to cover a march, you know, violently into legal jeopardy, because really this is about a journalist who has angered the most powerful and increasingly brutal nation on earth, and it's about all of us who are journalists being dragged into a dangerous situation because of criticism of the government.
AMY GOODMAN: Jaclyn Friedman, your response?
JACLYN FRIEDMAN: Wow. First of all, I've also been working with rape survivors for 20 years, and I am one myself. And I can assure you that you do not speak for me or many of us. I, too, have been speaking with rape survivors around the world since this case broke, who have been so hurt and disappointed that someone like you, who understands about the danger of perpetuating myths in the media, would be perpetuating rape myths that hurt all of us. There are so many rape survivors that are up in arms about the way this case has been discussed and the way these women have been disregarded.
NAOMI WOLF: But Jaclyn, Jaclyn, with all due respect—
JACLYN FRIEDMAN: I fully agree—no, no.
NAOMI WOLF:—where did they say no?
JACLYN FRIEDMAN: I did not interrupt you when you were speaking, and I would appreciate—
NAOMI WOLF: I beg your pardon.
JACLYN FRIEDMAN:—if you don't—I'm going get to that.
JACLYN FRIEDMAN: OK. So, I fully agree with you that the zeal, shall we say, with which these charges are being pursued is politically motivated. We have no disagreement on that. That is not an issue here. We are in agreement about that. I bet Amy agrees with us, too.
But if you want to talk about what the women in Sweden want, you should look at their political actions. There is a massive Twitter campaign that the women of Sweden have launched called "Let's Talk about It." I think that's right. It's translated from the Swedish, because they are all—
NAOMI WOLF: That's fair. Let's talk about it.
JACLYN FRIEDMAN: I'm talking about it right now. They are coming forward, and they're saying these things aren't taken seriously in Sweden, and this is an opportunity to prove that the Swedish government can take these issues seriously. This is an opportunity to set the international bar higher for the way we take seriously rape charges.
Now, let's talk about those charges. Those women did not consent. If she was consenting, he had no need to hold her down. A woman in her sleep cannot consent to sex. Consent is not a light switch, OK?
NAOMI WOLF: I have to speak to this.
JACLYN FRIEDMAN: Just because you've consented to choose one sexual activity, say, taking your clothes off with someone, does not mean you've consented to all sexual activities.
AMY GOODMAN: Naomi Wolf?
JACLYN FRIEDMAN: That's preposterous.
NAOMI WOLF: Yeah, I—
JACLYN FRIEDMAN: If I go home with someone, it's not consenting to every single thing that might be done to me by the person I've gone home with.
AMY GOODMAN: Jaclyn, let's get Naomi Wolf's—
JACLYN FRIEDMAN: Both women have clearly claimed that they did not consent.
AMY GOODMAN: We need to get—we only have a minute to go. We need to get Naomi's response.
NAOMI WOLF: Jaclyn, of course I agree with you that consent isn't a given and that obviously with every sexual act, everyone needs to be sure that everyone is consenting. There is no doubt about that. But I don't know if you've actually read the Guardian report, because again and again and again—
JACLYN FRIEDMAN: I most absolutely have.
NAOMI WOLF: Alright. So, again and again and again, Assange consulted with the women about what they wanted, and they didn't say no. And to me as a feminist—and this is why I'm hearing from so many rape victims around the world—and of course the issue needs to be discussed more, obviously, but the reason, as a feminist, I am distraught about this miscarriage of justice is that you can't—you're not respecting women by casting them as unable to assert what they want, unwilling, you know, to speak about what they wish. The women—
JACLYN FRIEDMAN: Women may be afraid.
NAOMI WOLF: Jaclyn—
JACLYN FRIEDMAN: If she's been held down by someone, she is afraid.
NAOMI WOLF: But wait, read—
JACLYN FRIEDMAN: She's in a state of fear.
NAOMI WOLF: Listen to me. Jaclyn—
JACLYN FRIEDMAN: No is not enough. Every sexual person—
AMY GOODMAN: We have 15 seconds.
JACLYN FRIEDMAN:—has the responsibility to get affirmative consent from their sexual partner.
NAOMI WOLF: He then consulted with her—
JACLYN FRIEDMAN: Not just no, but affirmative yes.
NAOMI WOLF:—and asked her what she wanted, and she did not say no. She continued to have sex with him. And what I'm saying is—
JACLYN FRIEDMAN: She was afraid. He had held her down. She was in a state of fear. Many, many women—this happens over—you talk to rape survivors. I can't believe you don't know this. Many women are in a state of fear and unable—they're in a panic situation. This is so common as to be laughable. And the reason they don't take these seriously is because you tell them that.
AMY GOODMAN: Ten seconds. We have 10 seconds. Naomi, you can respond.
JACLYN FRIEDMAN: I travel in the country and the world talking to rape survivors.
NAOMI WOLF: I mean, all I can say is if a man or a woman who's engaging in a sexual act that they think is consensual never hears "no" and hears "yes, yes, yes—yes, let's go ahead without a condom; yes, let's go ahead"—that insults rape victims.
the mean wrote: Can someone teach Sage to use the quote function?


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ALERT: Free Federal Donuts
PSA: Federal Donuts is currently giving away FREE donuts to all customers with a student ID. We repeat: FREE DONUTS. FREE! That's all, get back to the books.
Under The Button, your 24/7 source for all things Penn.
PSA: Federal Donuts is currently giving away FREE donuts to all customers with a student ID. We repeat: FREE DONUTS. FREE! That's all, get back to the books.
Given up all hope? Utilize the immense power of the geode! Don't know which geode to use? Our experts here have created a foolproof method of figuring it out. Take our quiz!
There's nothing worse than having a heartfelt goodbye with someone after your last final only to see them wheeling their stuff out of the Quad with their Mom three hours later. Do you say goodbye again? Do you just wave? Do they want you to meet their Mom? Are you that level of friends?
Sometimes there's no good way to say, "Have a nice life," but we've broken it down according to who you're saying goodbye to to eliminate some awkwardness.Read the Full Article
While many of us were hard at work studying for finals, one unnamed genius was busy creating the most important Instagram account known to mankind. @Yungbenfranklin, or "UPenn Memes", tackles everything from Theos pledge haircuts to Hubbub employee aesthetics. The account pretty much speaks for itself, so stop looking over your study guide and go get a quick laugh out of this thing.
Ed Note: To the gifted mind behind @yungbenfranklin: The editors at Under the Button would like to formally recruit you to become a member of our staff. Please get in touch with us at email@example.com at your earliest convenience.
Looking for a trendy new BYO spot because you've embarrassed yourself too many times at Banana Leaf? Desperately need to spend your last 300 dining dollars before the end of the semester?
The answer might be closer than you think. Yes, we're talking about Houston Hall.Read the Full Article
What up, Penn? I know you're used to reading UTB's third-person point of view – we are of the people! – but it's Charlotte Coran here, and rules don't apply to me. Follow along as I break down the things I will and won't miss about Penn in the last post I'll ever write for UTB.
10 Things I Won't Miss About Penn:
10 Things I Will Miss About Penn:Read the Full Article
It's already Wednesday and your outlook for the next couple of days is starting to mimic the eerily dim weather outside. But you know what Wednesday also means? That finals ARE ALMOST OVER! Soon, it'll officially be summer and you can head home, relax, and enjoy time with family and friends. And from May 27th-30th you'll be lucky enough to experience a marathon of 100 Disney Channel Original Movies. Remember when Troy Bolton tried to balance his love of basketball and his love of musical theater and his social life all at once??? Relatable, huh.
But we digress. Finals are tough. We read our notes over and over again and nothing sticks. We skip to the answers of our problem sets and say, "Oh, I definitely would have gotten that." And writing papers requires more dedication than a Hungarian student at Penn rushing Owls. But in a couple of days, you'll forget the difference between igneous, metamorphic, and sedimentary rocks. You'll forget how to calculate the present value of a perpetuity. You'll forget stressing about your grades and hopefully realize that finals are a tiny little microscopic thread in the XXL Snuggie that is your life.
Here's the hard truth: You'll do well on your exams, you'll get that paper done, and you'll think to yourself "why did I stress so much about that?"Read the Full Article
If finals weren't bad enough, filthy water is raining down on Harnwell residents. Six inches of water has flooded the 7th floor and has made its way down to the lobby. Apparently, Harnwell residents are being sent to hotels to avoid the disgusting water. You should pack an umbrella and bean boots if you plan to go there later. Happy finals!


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Report: Walmart shopper loads beer in buggy, races for the door
Published: Friday, January 25, 2013 at 3:15 a.m.
Last Modified: Saturday, January 26, 2013 at 6:26 a.m.
A man trying to steal beer assaulted store employees with a buggy, according to an incident report.
A manager at the Walmart on East Main Street told police that a man loaded 10 cases of beer — each contained 24 cans — into a buggy and tried to run out the door. The man ran into an employee with the buggy and knocked her down, the report states. The man used the buggy to assault another employee who attempted to help his coworker, before he managed to take the buggy away from the suspect, according to the report.
No employees were injured at the time the report was taken.
The suspect is described as a black male, between 55 and 60 years old, with a thin build, gray hair and of average height. He was last seen getting in a black, four-door vehicle where a driver was waiting and they fled.
Reader comments posted to this article may be published in our print edition. All rights reserved. This copyrighted material may not be re-published without permission. Links are encouraged.


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Encopresis Defined:
Encopresis is simply defined as fecal soiling at 4 years of age and beyond without any evidence of medical causes. A history and a ruling out of certain medical factors by a physician is recommended. Ninety-five percent of children are cleared of a medical cause and so encopresis is largely regarded as a functional disorder, much like many cases of high blood pressure.
Encopresis Treatment using the Soiling Solutions® Protocol:
Encopresis is normally a treatable condition. The Soiling Solutions Protocol for encopresis is the only home-based treatment approach that arrests fecal soiling in 90% of children within two weeks, causing dramatic and positive emotional changes in the child at school and at home. It enables the family to continue on a positive course with a daily treatment hour that gets shorter and shorter as the child's own body, and brain, gradually takes over full responsibility for successful, natural bowel movements. Take an "inside" look at the SS parents dealing with encopresis at www.encopresis.com/forum.
It is among Dr. Collins's contributions to scientific literature and knowledge that the necessary voiding reflex can be systematically re-arranged to take over from the overly dominant holding reflex… not by clean outs, talk, or persuasion… but by literally triggering it in a timely and efficient manner when the child has short sits on the toilet stool. This diagnostic condition of holding is called Functional Fecal Retention (FFR) and it is the dominant form of encopresis worldwide.
Please read on in this website to learn more about encopresis, what YOU can really do about it, and how you can put an end to this crisis in your life. Here you will have an opportunity to learn what the latest literature says about encopresis.
Upon purchasing the "Clean Kid Manual" in either physical or digital (pdf) form you will get on the path to success. You will have an opportunity to ask any questions that you may have on the SS® Parents' E-mail or Web Forum out to fellow "Mama Bears." There is an occasional rare dad on the forum! This is an extremely active forum of hundreds of parents who have used, or are using the SS® protocol. These parents never cease to amaze us with the help that they give to each other and the myriad of ideas they share to help others wit this challenge. Perhaps, truly … the answers are here.
FREE EXAM COPY: One copy only to a hospital department, medical clinic, physician practice, psychology practice, or school district. Click here to apply for your copy.


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Stephen LoBianco enters his third season at the helm of the Spartans after taking the reigns of the program in August of 2013. LoBianco had served as a Spartan assistant coach three years prior to him taking over the cross country and track & field programs.
LoBianco's 2010 inaugural season with the Spartan cross country squad proved a tremendous one as he saw Mandy Parshall capture York's first-ever individual conference championship in women's cross country. Additionally, he helped the Green and White to a pair of quality third-place team finishes at the annual Capital Athletic Conference Cross Country Championships.
During year two, LoBianco helped mentor Tim Hartung and Steven Childres, as they went 1-2 at the CAC Championships as the Spartans earned their first ever Cross Country championship. Hartung continued his great season with a ninth place finish at NCAA regionals. LoBianco also helped guide Hartung to York's first ever appearance in the NCAA Division III Championships.
As year three progressed, LoBianco saw the Men's Cross Country team win its second straight CAC Championships, with Hartung repeating as CAC individual champion. Hartung continued his success at NCAA Regionals where he placed fifth while helping the Men's team to a program history high finish of 7th. LoBianco then helped coach Hartung to York's first ever NCAA Cross Country All-American, as Hartung finished 7th at NCAA Cross Country Nationals.
In his first year as head coach, LoBianco has continued the success of the Cross Country program with many program firsts. For the third straight year, the Men's Cross Country team were CAC Champions and Hartung captured his third straight individual title. The team went on to tie the program's highest NCAA Regional finish with a seventh place finish, while for the first time in program history two athletes earned All-Region accolades. LoBianco was able to once again coach Hartung at nationals, where for the second year in a row he was able to become an NCAA Cross Country All-American with a 24th place finish.
On the women's side, there were many firsts as well. LoBianco coached Caroline DeLany and Jess Miller, as they went 1-2 at the CAC championships for the first 1-2 finish in program history in 2013. The success continued after the Women's team placed 8th at NCAA Regionals as a team, the first top ten finish in program history. LoBianco was able to see Miller receive All-Region accolades and be York's first female cross country runner to compete at NCAA Cross Country Nationals. In 2014, Miller made her second straight NCAA Championship appearance after she finished second at the CAC championships for the second straight year. Miller earned All-Region accolades and was joined by DeLany on the all-region squad.
As an undergrad at Shippensburg University, he was a four-year varsity athlete in cross country and track & field. LoBianco is a 2007 graduate of Shippensburg University with a BS in Business Administration.
After college he continues to run and train for local road races and track meets. He recently completed his first marathon in 2:35.05.
Updated: 8/5/2015


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Security, antivirus & spam help
All you need to know about avoiding security problems, fixing virus issues, and dealing with spam.
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Experience 'real' rest in meditation
Patanjali Yoga Sutras - Knowledge Sheet 15
Contd. from knowledge sheet 14
Continues from previous Patanjali Yoga Sutra post.
The four types of samadhi that we discussed are vitarkânugama samâdhi (the calmness that you get from special logic), vichârânugama samâdhi (equanimous state where you are aware of the thoughts, yet they are not disturbing you), anandnugama samadhi (blissful state) and asmitânugama samadhi (deep experience of meditation with just the awareness that 'you are'). All the four are called sampragnâtha.
Now, how to achieve these states of samadhi? The next Patanjali Yoga Sutra says it is very simple.
Viramapratyabhyasapurvaha samskara sheshonyaha
The practice of deep rest consciously happens (naturally) in some people due to old impressions. Just by doing something, you cannot achieve this awareness. You cannot bring up the intelligence or alertness in you by effort. This happens without effort, by relaxation, by reposing in the Self.
- Patanjali Yoga Sutra #18
Unconscious rest is sleep, which we are forced by nature. You are not resting; you are forced to rest. You are so tired and nature is pulling you down and forcing you and making you rest. Real rest happens only in deep meditation because you are consciously resting. You allow yourself to rest. Do you see the difference? Sleep is putting you to rest but meditation is you resting on your own. This is abhyasa (practice) of deep rest consciously.
Some people have to practice this in order to be equanimous and bring up more awareness in them. For some people it is by old impressions (samskaras) of past lives, births; it just happens. Sometimes right from the birth or sometimes at some particular period in life, they start opening up. This is samskara sheshonya. Have you noticed this? Some people, after 30-40 years, suddenly open up to some spiritual experience. They have more awareness. But unfortunately, most of those people get misguided. This is because there is no root of understanding of yoga. At that stage instead of getting misled by evangelic experiences, they should know that they are all from samskara shesha (old impressions). It is a different type of samadhi.
<< The States of Meditation Meditation Influences the Creation >>
(This is part of a series of knowledge sheets based on Sri Sri Ravi Shankar's commentaries on Patanjali Yoga Sutras.)


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A few notable media mentions for news.co.cr
UNESCO Int. Jazz Day (PDF) – April 30 2012
Archaeology.org – May 18 2012
TimesOfIsrael.com – June 12 2012
El Financiero – June 17 2012
SeaShepherd.org – June 27 2012
Panthera.org – August 12 2012
NaturalNews.com – December 7 2012
Ref 28 on Wikipedia – July 4 2013
BiologicalDiversity.org – July 31 2013
CRHoy.com – August 3 2013
LatinTimes.com – August 5 2013
InfoWars.com – August 7 2013
GMWatch.org – August 21 2013
QCostaRica.com – August 25 2013
Care2.com – August 31 2013
Quartz (QZ.com) – September 5 2013
BBC News – October 21 2013
The Costa Rica Star will blur the line between Tico and foreigner, and bring everyone interested in Costa Rica together to empower and educate in a transparent fashion, through the digital publication of English news media.
The Costa Rica Star publishes news articles across 10 categories, mainly focusing on Costa Rica local news with occasional content from Latin America and World News.
We are listed in Google News globally and thus receive thousands of daily website hits from all nations of the world who have interest in Costa Rica.
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Why should YOU read and subscribe to The Costa Rica Star? Because you will come away empowered and educated on not only the current events in Costa Rica, but you will get a chance to shape English news media here through your feedback.
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MONTGOMERY, Ala. (AP) -- The National Trust for Historic Preservation is recognizing the agencies that preserved Montgomery's historic Greyhound Bus Station and made it into a museum honoring the Freedom Riders from the civil rights era.
The National Trust will present its award for Federal Partnerships in Historic Preservation to the General Services Administration, the U.S. District Court, the Alabama Historical Commission and the Greyhound Bus Station Advisory Committee on Thursday in Montgomery.
The bus station now houses the Freedom Rides Museum.
It honors civil rights activists who were attacked by a mob at the station in 1961 because they were integrating interstate bus travel.
The award was officially presented by the National Trust last month in Spokane, Wash., but Thursday's ceremony in Montgomery is an opportunity to involve participants in the project.


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MOUNTAIN VIEW -- A Google-affiliated company will restore Moffett Federal Airfield's historic Hangar One and manage the airfield for the agency.
Planetary Ventures LLC will restore and maintain Hangar One, restore Hangars 2 and 3, upgrade the golf course, create an educational facility and help turn Moffett's airfield into "a viable asset to support government and controlled public and private flight operations," NASA announced Monday.
In a statement, Dan Tangherlini, administrator for the federal General Services Administration, called Hangar One "the landmark of Silicon Valley well before the rise of today's high tech titans. ... NASA's partnership with the private sector will allow the agency to restore this treasure for more efficient use.'' He also said the agreement will create new "opportunities for development while eliminating NASA's management costs of the airfield and saving taxpayer dollars."
NASA Administrator Charles Bolden said in a statement that the agreement "will allow NASA to focus its resources on core missions, while protecting the federal need to use Moffett Field as a continued, limited-use airfield."
Contact Dan Nakaso at 408-271-3648. Follow him at Twitter.com/dannakaso.


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Workplace Surveys
GSA surveys federal customers in GSA owned and leased facilities throughout the nation, using the Tenant Satisfaction Survey. This survey aims to elicit feedback regarding their work environment experiences. Survey topics include building cleanliness, indoor air quality, security, and overall satisfaction with the space and with GSA.
In 2014, most federal tenants will receive a paper survey which can be completed and mailed to GSA, or completed online. Some federal tenants will be surveyed via the web and will receive an email from Gallup.
This feedback (acquired from approximately 8,300 facilities nationwide) provides GSA with accurate and timely information to use in a continuing effort to better serve its customers. Surveying the federal workforce is essential to anticipating, understanding, meeting, and exceeding federal tenants' business and workspace needs.


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Photoshop offers an impressive array of tools for editing digital photos, scanned photographs and creating photorealistic images. This is an intensive, hands-on workshop. You will be introduced to Photoshop's powerful capabilities including selection techniques, image retouching and restoration, and using layers and masks to your advantage.
Bring a USB flash drive (minimum 2 GB) to class.
Prerequisites: A strong working knowledge of your computer's operating system with the ability to create and rename files and folders and navigate through multiple drives and directories.
See our detailed course information including course content, learning objectives, and prerequisites.