text:
Type hinting helps the compiler to assume the type of the variable, but, as the PHP is a dynamic scripting interpreted language, the question came to my mind if it's possible that type hinting even makes the runtime faster or not?
We started with Q&A. Technical documentation is next, and we need your help.
Whether you're a beginner or an experienced developer, you can contribute.
PHP is a dynamic language.
Type hinting only hinders performance because it requires (for objects) to test the inheritance hierarchy. To make things worse, this kind of check is expensive in PHP because it is done in time proportional to the depth of the hierarchy.
A test for type, such the one done for the
Furthermore, it is currently not used for any optimization purposes.
It is however, a useful defensive programming feature.
All type hinting in PHP does is add code that tests the type of the parameters and fails if its not what's expected, so type hinting NEVER helps performance. Its only real use is for debugging, so if you have a function that's called very often, removing the type hint in the production code can speed things up, especially since checking the type of an object isn't the fastest thing in the world.
also see when should I use type hinting in PHP? and abusing type hinting in PHP


text:
As a 16-year-old, I may be going against what my peers think.
I believe our government is spending way too much.
Our national debt is $13 trillion.
I don't want my generation and the generations to come to be saddled with so much debt.
I am very concerned with what my government is doing. Where has all the stimulus money gone? Where are the new jobs it was supposed to produce?
Will continued spending and new taxes increases really help our economy recover or will they further exacerbate this problem? I think lower tax rates and reduced government spending would truly stimulate our economy.
It's time our Democratic and Republican representatives stop spending our money to ensure their political future.
Ben Huebner
Amarillo Globe-News © 2016. All Rights Reserved. Terms of Service | Privacy Policy / About Our Ads

subset: commoncrawl


text:
Site top menu
Secondary menu
A round of tweaks to the Lamborghini Gallardo has produced the faster, sharper and more eye-catching LP560-4
We get behind the wheel of the facelifted Gallardo to deliver our verdict
Italian's Gallardo Spyder has lost some weight – but has that given it a performance boost?
More power, less weight – we see if this thrilling recipe has produced the most sensational raging bull ever.
Stripped-out 570bhp monster is the Italian marque's ultimate supercar – and we've driven it!
Firm's new entry-level model returns to two-wheel-drive roots
Don't be deceived - despite appearances the entry-level Lambo features a raft of upgrades
Featured in: Welcome to Greatest Drives 2007
The Lamborghini was the most expensive car on test. Luckily the super-light Gallardo was also the most rewarding to drive
The Gallardo Superleggera nearly matches the Porsche 911 for sheer excitement and style
Sensational Italian supercar nearly does it all - and wraps that considerable talent in a fantastic-looking body. A modern-day masterpiece
Auto Express Editorial Offices
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© Copyright Dennis Publishing Limited.Auto Express is a trademark of Felix Dennis.


text:
Submitted by Brittany
Share More
Amber Burson Lycett:
October 5, 2012 at 4:27 am
I had one of these days today!
Katie Slavnik:
October 6, 2012 at 3:16 am
I want to go toddler today!
Filipe Barreiros:
October 7, 2012 at 12:06 am
Going toddler sounds like a great idea sometimes :D
Didi Paris:
October 7, 2012 at 12:20 am
Theat IS the best autocorrect... lol
Katie Foreman:
October 7, 2012 at 12:37 am
To REALLY go toddler, you have to have a tantrum because the juice was 2 seconds late.
"Best of" Lists


text:
7th night free when you stay between 11/23/16 - 4/1/17. Promotion is blacked out 12/24/16 - 1/2/17.
Minimum night stay requirements and other restrictions may apply. Properties displayed on this site are subject to availability at time of purchase. Valid on new reservations only. Promotion cannot be combined with any other offer.
Designed to reflect the charm and subtle elegance of the fine country
inns of Bavaria, The Alpenhof is a place where travelers, skiers, or
hikers arrive to find warmth and hospitality and is in close proximity
to all ski lifts and in front of the public transportation system that
operates daily to the town of Jackson.Though small, it offers all the comforts of a
full-service, upscale hotel. We have two restaurants and a bar. Guests
will enjoy our outdoor heated pool and hot tub. We have a dry sauna and
offer spa services. All room rates include a Continental Breakfast.
Bavarian Rooms are more spacious than Dolomite rooms and are furnished with hand crafted Bavarian furniture. This room would compare to a standard sized hotel room. Rooms are available with two double beds or one king bed. Some adjoining rooms are available, offering privacy and space for a family.
Photos are representative of the property and may differ from the actual unit that is reserved.
For more detailed information about the amenities for this property please call one of our
Destination Experts at 800.948.6416
NOTE: Calendar displays standard nightly rates. Please see above for our best pricing applied to the dates you selected.
Clear Dates
Looking for more information on this property or have questions on planning your vacation? Our friendly
Destination Experts are here to help. They will usually respond to your request within 5 minutes
to the submission of this form.
We are excited to help you with your vacation
and will contact you as quickly as possible, often
within just a few minutes.
Our agents live, work and play in the destinations we offer, ensuring you benefit from local expertise while planning your trip.
They went the extra mile to offer me the most up to date rates and availability for my vacation.
Their staff was very knowledgeable and quick in response to all my questions. I would book with them again!
-Shauna K.


text:
I upgraded to WordPress 3.04 and, once again, WP Stats presented its challenge. As usual, I chose the wrong option. I can see the stats I want in the "global desktop," but they don't show up on the blog desktop. How do I switch?
I hope the question is clear. I'm about ready to toss WP Stats out for good just to avoid this problem. WP Stats' explanation of the choices after an upgrade is always confusing, and in about three of the last five upgrades I haven't been able to remember which choice was correct and I made the wrong one. And once that's done, you're screwed.
WP Stats doesn't even offer the option to grab the old stats and leave. Google Analytics looks better all the time.


text:
Video Industry News Video Company News Technology Industry News Technology Company News Novelty Industry News Novelty Company News Gay Industry News Gay Company News Legal Industry News All Company News
Released: Jul 01st, 2005
Running Time: 96
Director: Frank Thring
Company: H2 Video
Distribution Companies: LFP, Inc.
Cast: Honey Winter, Karla Romano, Clark (I), Sziszi, Ricardo Bell, Vyona, Kitty Sixx, Barbi, Nicole Desire, Jenny Sanders, James (I), Thomas Stone
Critical Rating: AAA
Genre: All-Sex
Often zany themes come to life in this import. Good for couples.
Various fantasies are acted out - to the obvious amusement of the viewers and, frequently, participants.
After exploring the possibilities of sex in a bathroom, library and health spa, during which anal does occur, one hapless dude decides that he needs to don his wife's clothes, wear make-up and get laid. Cute gal - well worth the kidding he probably got from his friends afterwards.
The craziest theme is titled "I Screwed the Xmas Tree Fairy," and yes, the fairy naturally comes to life, plays with his wand and rides him until New Years. OK, not quite that long.


text:
Most Popular Hotels in Lewes
Get 200 Nectar points when you book your hotel
Simply enter your Nectar card number when prompted. Your points will be credited 30 days after you return from your trip.
The Newmarket Inn
Brighton Road, Lewes BN7 3JJ2.5 out of 5.0
Situated in Lewes, this inn is 1.6 mi (2.6 km) from Anne of Cleves' House and 2.8 mi (4.5 km) from University of Sussex …
The White Hart Hotel
55 High Street, Lewes BN7 1XE3.0 out of 5.0
Located in the heart of Southerham, this hotel is steps from Lewes Castle and Barbican House Museum. Southover Grange Gardens …
The Black Lion Inn
Lewes Road, Lewes BN8 6PN3.0 out of 5.0
Located in Halland, this inn is 1.8 mi (2.9 km) from East Sussex National Golf Club and 2.8 mi (4.5 km) from Bentley Wildfowl …
The Blacksmiths Arms
London Road, Lewes BN7 3QD4.0 out of 5.0
Situated in a rural location, this inn is within 3 mi (5 km) of Southover Grange Gardens, Lewes Castle, and Barbican House …
The Bull at The Green
The Green, Lewes BN8 4LA3.0 out of 5.0
Situated in Newick, this inn is 2.5 mi (4 km) from Piltdown Golf Club and 2.9 mi (4.7 km) from Sheffield Park Garden …
The Forge
Near Lewes, Lewes BN8 6PW3.0 out of 5.0
Located in Halland, this inn is 1.9 mi (3 km) from East Sussex National Golf Club and 2.9 mi (4.6 km) from Bentley Wildfowl …
YHA South Downs - Hostel
Itford Farm, Beddingham, Lewes BN8 6JS4.0 out of 5.0
Situated in Lewes, this hostel is 1.6 mi (2.5 km) from Monk's House and 2.6 mi (4.2 km) from Newhaven Museum. Newhaven Fort …
The Roebuck Inn
Lewes Road, Lewes BN8 6BG3.5 out of 5.0
Situated in Lewes, this inn is within 6 mi (10 km) of Glyndebourne Opera House, Michelham Priory, and East Sussex National …
White Lodge B&B
Town Littleworth Road, Lewes BN8 4TD4.0 out of 5.0
Situated in a rural location, this bed & breakfast is within 6 mi (10 km) of Piltdown Golf Club, Sheffield Park Garden, and …
The Shelleys Hotel & Restaurant
136 High St, Lewes BN7 1XS4.0 out of 5.0
Located in the heart of Lewes, this hotel is within a 15-minute walk of Southover Grange Gardens, Anne of Cleves' House, and …
Recent Reviews in Lewes
Nice Hotel situated at Gatwick Airport BUT !!
A verified traveller from Ontario , Canada stayed at Courtyard by Marriott London Gatwick Airport
Expedia Verified Review
A verified traveller stayed at Ramada Crawley Gatwick
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text:
By Directorate of Communication and Public Affairs, U.S. Army Combat Readiness/Safety CenterJune 5, 2014
During National Safety Month 2014, the U.S. Army Combat Readiness/Safety Center will highlight achievements and perspectives of members of the Army's first class of Career Program-12 safety interns, who finished training in 1984. Patti Tilson, manager of a safety and environmental team for the Federal Aviation Administration's Eastern Service, is the first intern profiled for this series.
What drew you to a career in safety?
My interest in occupational safety stemmed from an accident I had while working a college summer job at a manufacturing plant. One of my tasks involved putting a bottle under a clamping device. If you didn't line the tops of the bottles correctly, they would explode into sharp pieces. Unfortunately, I had a bottle explode in my hand, and the wound required multiple stitches. When I returned a day later, the machine had a Plexiglas case installed around it with a small opening for a hand. The engineer had fixed the problem of flying glass, but workers' hands were still exposed to the hazard. I thought there had to be a better answer.
At that time, the Occupational Safety and Health Act was only about 8 years old, and my university had just begun offering a master's degree in safety management. Based on my experience with on-the-job injury, I was very interested in the concept of a new career field that sought to effectively manage health and safety risks.
What was the most important thing you learned or took away from the CP-12 training program?
The CP-12 program was just an idea when I was hired. I was actually recruited from my university and selected as one of Army's first safety interns. My class arrived at Fort Rucker, Alabama, in 1984 and began working and training with some of the Army's most experienced military and civilian safety professionals. The men and women at the Army Safety Center shared their experience and knowledge to ensure we had access to the best on-the-job training possible. We participated in sophisticated accident investigations, explosives training, program reviews, incident analysis and construction safety.
The program was expanding and a new class was beginning the following year, so our internship continued when three of us transferred to U.S. Army Europe. There I worked with incredibly dedicated safety professionals who taught me about field hazards, motor pools, river crossings, rail loading and fire protection. That field experience provided the foundation for my safety career.
As a senior safety mentor, what do you try to inspire in younger safety mentees?
Safety professionals are in the unique position of representing both management and labor interests. You must constantly balance the needs of the organization to complete the mission cost effectively, while simultaneously ensuring employee safety. Your job is to develop and integrate the risk controls necessary to protect personnel and assets. It requires special skills to communicate critical facts and identify what risk can be accepted. All risk is not created equal.
To address high risk factors, you should use all your tools and develop a comprehensive plan to address the task. In cases of low risk, you empower the task manager to work the issue and require local actions.
Safety is more than slogans and compliance regulations. What is your safety philosophy?
Being a successful safety manager requires a mutual respect for the mission, safety standards and employees. I have a basic philosophy of more safe/less safe/unsafe, but less safe does not equal unsafe. In days of shrinking budgets, financial resources have to be deployed in the most cost effective manner, and even the safety program must be strategic in how we prioritize and execute compliance initiatives. Our most successful initiatives have phased implementation, upper level management support and measurable metrics in common. Phased implementation enables our team to evaluate and assess what works and then improve any aspect that requires adjustment. This approach allows management to trust the plan and assist us with measuring achievement through metrics. When management considers safety professionals an integral part of the team, everyone is successful, the mission is accomplished, and employees comes back to do it again tomorrow.
(In addition to her professional achievements, Tilson holds a master's degree in safety management, bachelor's degree in business administration and the Project Management Professional credential.)


text:
Help,Reading Labels?
Over a year ago
I've been informed that even some non dairy things have
traces of animal products in them,What are all of the additives
and other Things that are put into food,Listed on the back of the labels,
That a beginning Vegan like myself,should avoid and watch out for?
Please log in to reply.
Over a year ago
Here's a list that may also be helpful: http://www.peta2.com/lifestyle/animal-ingredients/You must be logged in to reply to this topic.
Over a year ago
Thank you both! This info is very informative,I'll make sure to make some notes
about what I need to look out for,And carry it with me in my purse when I go shopping
next time, thankfully most of what I bought last time is all vegan,save for these meatless soy non chicken nuggets that actually contain the egg whites as a binder in the batter
Boo! 🙁 I gotta watch out for this stuff. Thanks again!You must be logged in to reply to this topic.
Over a year ago
When I first went vegan, I made this wallet-sized card:
And I used it whenever I was unsure about ingredients. It's not a complete list but I think it's the most common things.You must be logged in to reply to this topic.
Over a year ago
Casein is a animal based protein (it comes from milk), which is often included in soy cheeses as well. Rennet, unless specified as vegetarian rennet is an enzyme from a cows stomach. A lot of products list albumen, which is a fancy term for egg whites. Carmine is a red pigment made from crushed mites… Though if you are eating any factory made food you are probably eating bug parts. Health and safety has a list which says the maximum numbers of bug parts a food can contain. Gelatin is made from the boiled connective tissues of animals. Those are the most common ones you see. If you have an iPod there is an app which lists all sorts of stuff, and what it's made from and lets you sort always vegan/sometimes vegan/not vegan friendly.You must be logged in to reply to this topic.


text:
Mitsakoz1989 (Talk | contribs)
(Created page with "Ο πλήρης κατάλογος της λίστας e-mail του KDE είναι διαθέσιμος [http://www.kde.org/mailinglists/ εδώ] και [http://mail.kde.org/mai...")
Mitsakoz1989 (Talk | contribs)
(Created page with "Μια πιο λεπτομερής περιγραφή των βημάτων παραπάνω είναι διαθέσιμη στον [http://quality.kde.org/develop/howto/howtohac...")
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[[Development/Tutorials/API Documentation|KDE API Reference Manuals]] section, which also contains a number of useful links on how to write or update the class documentation. You can also generate these on your own machine, or refer to a more up-to-date online version at [http://api.kde.org/ API Reference].
[[Development/Tutorials/API Documentation|KDE API Reference Manuals]] section, which also contains a number of useful links on how to write or update the class documentation. You can also generate these on your own machine, or refer to a more up-to-date online version at [http://api.kde.org/ API Reference].
[http://quality.kde.org/develop/howto/howtohack.php ].
== Getting Involved in Bug Hunting and Application Quality ==
== Getting Involved in Bug Hunting and Application Quality ==
Languages: عربي | Asturianu | Català | Česky | Kaszëbsczi | Dansk | Deutsch | English | Esperanto | Español | Eesti | فارسی | Suomi | Français | Galego | Italiano | 日本語 | 한국어 | Norwegian | Polski | Português Brasileiro | Română | Русский | Svenska | Slovenčina | Slovenščina | српски | Türkçe | Tiếng Việt | Українська | 简体中文 | 繁體中文
Αυτή η σελίδα έχει σκοπό να δώσει μια γενική εικόνα των διαφόρων απόψεων πάνω στην ανάπτυξη KDE και κυρίως σε θέματα προγραμματισμού. Το έργο KDE καλωσορίζει όποιον επιθυμεί να βοηθήσει.
|Υπάρχουν πολλοί τρόποι να συνεισφέρει κάποιος στην ανάπτυξη του KDE, οι οποίοι σε γενικές γραμμές είναι οι εξής:
Οι γενικές κατευθύνσεις του έργου KDE αποφασίζονται από αυτούς που κάνουν τη δουλειά - δεν υπάρχει κάποιο σχέδιο για το πώς θα δείχνει το KDE μελλοντικά.
Εάν επιθυμείτε να γνωρίζετε τι συμβαίνει αυτή τη στιγμή, υπάρχει πλήθος πηγών να χρησιμοποιήσετε:
The easiest way to contribute to KDE is to report any bugs you find in KDE using the KDE Bug Tracking System (also known as Bugzilla).
If the application you are using crashes then the Dr Konqi utility will appear and guide you through the process of reporting the crash. Learn more by reading how to create useful crash reports.
Getting started at coding for KDE is a matter of finding something to fix, and fixing it. You may want to consult the module overview to help find what you are looking for; once you have fixed something, you will want to send in a patch. If you do that a few times, you may want to apply for a KDE Contributor account so you can improve things directly.
KDE is mostly written in C++. If you are not familiar with C++, you should do at least some work on it. There are a number of good books on C++ - an excellent source is Bruce Eckel's "Thinking in C++", which is available both as a free download and as a printed document. It isn't essential to understand everything before you start in KDE, but you do need to understand basic syntax and operations.
To become proficient with KDE coding, you should understand the Qt toolkit. If you are not familiar with Qt, you should work through the tutorials included with Qt Reference Documentation.
If you need a gentler introduction to Qt, or would just like an alternative view, then you may wish to look at the The Independent Qt Tutorial (Currently offline due to book contract).
If you prefer to learn Qt by reading a traditional book, take a look at the Books about Qt page. More suggestions on becoming familiar with Qt4 are available How to Learn Qt page.
A range of information on KDE techniques is available in the tutorial section. Note that some of these tutorials still target KDE3, though they should be at least partly applicable.
You will also find useful information on KDE coding in the FAQs section. This information may also be somewhat dated for KDE4, however much of it is broadly applicable, even outside KDE.
Μπορείτε επίσης να διαβάσετε Βιβλία ανάπτυξης κώδικα KDE.
Last, but by no means least, KDE comes with extensive class (Application Programmer Interface) documentation. This is available in the KDE API Reference Manuals section, which also contains a number of useful links on how to write or update the class documentation. You can also generate these on your own machine, or refer to a more up-to-date online version at API Reference.
Μια πιο λεπτομερής περιγραφή των βημάτων παραπάνω είναι διαθέσιμη στον οδηγό προγραμματισμού.
There is a large number of applications within KDE, and not all of them have a maintainer dedicated to managing bugs and generally helping out with all the issues associated with turning some working code into a polished application.
If you are interested in helping out with KDE, but don't know where to start, becoming a member of the KDE Quality Team might appeal to you - see the Quality Team website for more information. Note that you do not need any programming skills to become involved. In particular developers regularly publish so-called Junior Jobs to encourage new contributions.
Of course, you can become involved in bug hunting without being part of the KDE Quality Team - just create yourself an account on the KDE bug tracking system, and start searching / sorting through the bugs. Again, you don't have to have programming skills - it helps the programmers enormously just to have a procedure that allows a bug to be consistently reproduced.
The Bugsquad tries to keep track of bugs in KDE software and make sure that valid bugs are noticed by developers. You do not need any programming knowledge to be in the Bugsquad; in fact it is a great way to return something to the KDE community if you cannot program.
If your question concerns KDE development, your options are pretty much the same general user ones, with some modifications:
- Read the Developer FAQ. Many common developer questions have been answered in the KDE Developer FAQ
- Search/browse KDE websites. A lot of questions can also be answered from the KDE websites, and the documentation included on it. You can search all the KDE websites on the homepage. In addition, you can browse the KDE TechBase website. And if possible, help edit it for clarity, and use the talk page if something is unclear.
- Search mailing lists. A lot of questions have already been answered on the KDE mailing lists, particular the lists kde-devel, kde2-porting, kde-core-devel, kde-games-devel, kfm-devel and koffice-devel. You can search these lists either at lists.kde.org. You should always search for your answer before asking questions on the mailing lists. When you ask a question on a mailing list you are emailing thousands of people -- please do this only if the answer is not available through a simple search.
- Search engines. Do not forget about your favorite search engine. One of the best search engines is Google. With Google you can also search the great bulk of Usenet news sites, which is also particularly helpful, especially for general programming and gcc-related questions.
- Read the source code. http://websvn.kde.org and https://projects.kde.org/ are available to help browse code. Read some commit logs and diffs for the code you might want to work with, It adds perspective.
- Ask on KDE mailing lists. If you still do not have an answer, try asking your question on one of the KDE mailing lists listed above.
- For questions relating to core development or third-party KDE development, unless you are particularly interested in Konqueror, KOffice, games or Java development, your main choice is kde-devel (subscribe).
- For questions relating to Konqueror development, your main choice is kfm-devel (subscribe)
- For questions relating to KOffice development, your main choice is koffice-devel (subscribe)
- For questions relating to games development, your main choice is kde-games-devel (subscribe)
- For questions relating to Qt development, please use the fine Qt mailing list.
Ο πλήρης κατάλογος της λίστας e-mail του KDE είναι διαθέσιμος εδώ και εδώ.


text:
Laws &c., Cambridge Camden Society (1839) · J. M. Neale, diary, LPL, MS 3107 · E. J. Boyce, A memorial of the Cambridge Camden Society (1888) · G. Brandwood, The establishment of the [Cambridge Camden] Society and A Camdenian roll-call, A church as it should be: the Cambridge Camden Society and its influence, ed. C. Webster and J. Elliott (2000), 4561; 359454 · J. F. White, The Cambridge movement: the ecclesiologists and the Gothic revival (1962) · A. J. B. Hope, The present state of ecclesiological art in England, Proceedings of the Oxford Society for Promoting the Study of Gothic Architecture (1846), 1932 · B. M. G. Reardon, Religious thought in the Victorian age: a survey from Coleridge to Gore, 2nd edn (1995) · C. Webster, ed., Temples ... worthy of His presence: the early publications of the Cambridge Camden Society (2003) · The Ecclesiologist, 129 (184168) · A few hints on the practical study of ecclesiastical antiquities (1839) · A few words to church builders (1841) · A few words to churchwardens on churches and church ornaments, 1: Suited to country parishes (1841) · A few words to churchwardens on churches and church ornaments, 2: Suited to town and manufacturing parishes (1841) · A few words to the parish clerks and sextons of country parishes (1843) · Church enlargement and church arrangement (1843) · The history of pews (1841) · A handbook of English ecclesiology (1847) · J. M. Neale and B. Webb, eds. and trans., The symbolism of churches and church ornaments: a translation of the first book of the Rationale divinorum officiorum written by William Durandus (1843) · J. F. Russell, ed., Hierurgia Anglicana, or, Documents and extracts illustrative of the ritual of the church in England after the Reformation (1848) · D. Watkin, The rise of architectural history (1983) · J. M. Crook, The dilemma of style (1987)
|© Oxford University Press 2004–16
All rights reserved: see legal notice
Christopher Webster, Cambridge Camden Society (act. 18391868), Oxford Dictionary of National Biography, Oxford University Press, Jan 2016 [http://www.oxforddnb.com/view/theme/96307, accessed ]
|Site credits


text:
Charles Lao
I obtained my PhD in Cognitive Neuroscience at the University of Glasgow. My thesis is about the modulation of high-level social inferences (i.e., Culture) on visual perception. The technique I applied including eye tracking, EEG, and fMRI.
I am currently working as a post-doc in the University of Fribourg. I am interested in Bayesian Reasoning and machine learning, especially the application of these methods in naturalistic paradigm.
- Fribourg, Switzerland
- unifr.ch/psycho/staff/lao-junpeng
- Member for 2 years, 1 month
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- Last seen Oct 26 '15 at 8:22
Keeping a low profile.
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Recent Appearances
Connecticut Senate Debate
Candidates Linda McMahon (R) and Chris Murphy (D) met in their fourth debate for retiring Senator Joe Lieberman Senate seat.
Connecticut Gubernatorial Debate
The four gubernatorial candidates debated Connecticut and national issues. They were questioned by…
Connecticut Politics
The guests discussed the political issues important to Connecticut's residents and responded to callers'…


text:
Juvenile Arthritis Center - Yuba City, CA
- WebMD Physician Directory of Yuba City Pediatric Rheumatologist Doctors
- Related Juvenile Arthritis Articles
- Yuba City Emergency Contacts
- Nearby Yuba City Hospitals
- Find a Local Pharmacy on RxList - Yuba City
Yuba City Pediatric Rheumatologist Doctors for Juvenile Arthritis
Type of Physician: Pediatric Rheumatologist
What is a Pediatric Rheumatologist? A subspecialty certification by the Board of Pediatrics; practitioners treat the infant, child, or adolescent with rheumatic disease (causing painful disability in the muscles, tendons, bones, joints or nerves). He or she also participates in the prevention, understanding, and treatment of the various rheumatic disorders and also promotes understanding of the basic mechanisms of the disease in the interrelated areas of immunology, genetics, inflammation, and infectious diseases.
Specialty: Pediatric Rheumatology
Common Name:
Pediatric Rheumatologist Doctors in Yuba City * results
Don't see your city? Use the WebMD Physician Directory to expand your search.
Juvenile Idiopathic Arthritis (Juvenile Rheumatoid Arthritis)
Juvenile idiopathic arthritis (JIA) facts
- Epidemiologic studies estimate that approximately 294,000 American children are affected by JIA.
- Children of European ancestry are more likely to develop the condition while those of...
Read the Juvenile Idiopathic Arthritis (Juvenile Rheumatoid Arthritis) article »
Other Related Juvenile Arthritis Articles
Emergency Contact for Yuba City
- In case of Emergency, call 911
Nearby Yuba City Hospitals results
- 1Fremont Medical Center
970 Plumas St
Yuba City, California 95991
(530) 751-400
- 2Sutter-Yuba Mental Health Svs
1965 Live Oak Blvd Ste A
Yuba City, California 95991
(530) 822-720
- 3Rideout Memorial Hospital
726 4th St
Yuba City, California 95901
(530) 749-430
- 4Biggs-Gridley Memorial Hospital
240 Spruce St
Yuba City, California 95948
(530) 846-567
- 5Colusa Regional Medical Center
199 E Webster St
Yuba City, California 95932
(530) 458-582
- 6Oroville Hospital
2767 Olive Hwy
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text: Everlasting Gold Heart Charm Hoop Earrings by Melinda Mulcahy £45$80.56€54.28$60.39 In stock £18.50$33.12€22.31$24.83 delivery to United States Receive this item 5-10 working days from dispatch International delivery available add matching bangleadd matching bangleEverlasting gold heart on bangle (+ £36.50)add matching bangleEverlasting gold heart on bangle (+ $65.34)add matching bangleEverlasting gold heart on bangle (+ €44.02)add matching bangleEverlasting gold heart on bangle (+ $48.99) quantity by Melinda Mulcahy Location: Vale Of Glamorgan, GB read seller's profile see seller's complete range more items from this seller Personalised Initial Beanie Charm Bangle by Melinda Mulcahy £42$75.19€50.66$56.37 Everlasting Gold Heart Charm Bangle by Melinda Mulcahy £39.50$70.71€47.64$53.01 Freedom Feather Pearl Silver Earrings by Melinda Mulcahy £29.95$53.61€36.12$40.19 Freedom Feather Pearl Gold Earrings by Melinda Mulcahy £36.50$65.34€44.02$48.99 Love Fix Silver Heart Necklace by Melinda Mulcahy £28$50.12€33.77$37.58 Morocco Silver Earrings by Melinda Mulcahy £59.50$106.52€71.77$79.86 view all you may also like PRODUCT DETAILS CUSTOMER REVIEWS DELIVERY RETURNS Made in Britain ask seller a question save for later Inspired by the golden reflections of the sunset in Bali. When less is more! These lovely hoop earrings offer great presence yet are much lighter then they appear. Puffy gold heart charm hoops make a fabulous fashion statement, day or night. Exclusively created by Melinda Mulcahy, they will arrive beautifully gift boxed, tied with ribbon and gift tag so they are ready to give. 24ct Gold Vermeil handmade love heart charms dangle from a pair of easy fitting Sterling Silver hoops. Slip off the hearts if you want a little less. Add the matching bangle to make it a set and save £3. Stunning, timeless, simple. made from: Sterling Silver hoops. Charms are 24ct gold micro-plated on sterling silver. dimensions: Hoops measure 5cm + heart 1.5cm. Total drop is 6.5cm. product code: 200672 1 customer review read about our product rating system ratings guide trusted independent customer feedbackforum excellent bad good poor This product is dispatched to you by Melinda Mulcahy. MAINLAND UK order now and receive this item by price Free Delivery Wednesday 27 July FREEFREEFREEFREE NON-MAINLAND UK estimated delivery price Highlands & Islands of Scotland 2-5 working daysfrom dispatch FREEFREEFREEFREE Northern Ireland 2-5 working daysfrom dispatch FREEFREEFREEFREE Other UK Islands 2-5 working daysfrom dispatch FREEFREEFREEFREE INTERNATIONAL DELIVERY estimated delivery price Western Europe 2-5 working daysfrom dispatch £11.50$20.58€13.87$15.43 Rest of Europe 5-10 working daysfrom dispatch £11.50$20.58€13.87$15.43 USA & Canada 5-10 working daysfrom dispatch £18.50$33.12€22.31$24.83 Asia & Australasia 5-10 working daysfrom dispatch £19.50$34.91€23.52$26.17 Rest of the world 5-14 working daysfrom dispatch £19.50$34.91€23.52$26.17 You have 28 days, from receipt of cancellable goods, to notify the seller if you wish to cancel your order or exchange an item. Please note: goods that are personalised, bespoke or made-to-order to your specific requirements, perishable products and personal items sold with a hygiene seal (cosmetics, underwear) in instances where the seal is broken are non-refundable, unless faulty. Read more about our returns policy.Should you choose to return or exchange your order you will need to deliver the item(s) to the UK, where this seller is based. Melinda Mulcahy's return policy Please see notonthehighstreet.com's returns policy.


text:
I installed this plugin yesterday, and today my blog was locked down by my host for "excessive resource" usage (typically vague).
I haven't added any new plugins or done anything major to site site for a few weeks, so the immediate culprit is this plugin.
Anyone else seeing these sorts of issues?


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Sorry! The position you're looking for is no longer available. Check out other Event Planning internships in New York, NY below.
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Samantha Vazquez — New York, NY
July 28 2016
6up is a full-service event design and production company looking for two reliable and motivated people to join our team. You will be assisting on numerous fashion, art and charity events starting Aug…
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What Were Looking For We have openings for college undergraduates and graduates who can work on a Full-Time and Part-Time basis. Our goal is to provide you with valuable experience and guidance that c…
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Bar Crawls Intern NYC - Fall 2016
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BarCrawls.com is seeking an outgoing, motivated individual in our New York City office to support our Marketing & Events department. Assisting Event Managers with venue sourcing in multiple markets (N…
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Space In The Raw provides unique venue and event business solutions to New York and LA's high-end market by connecting desirable clients with hidden and underutilized real estate properties. Compensat…
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Colin Cowie Celebrations is seeking a self-starting and passionate Event Production intern. This internship will provide first hand experience in planning events and assisting with production tasks. S…
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Event Management Agency — New York, NY
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We look for students who are passionate, dedicated, energetic, resourceful and creative. We seek students who possess fundamental skills and the desire to launch their career into the future. In this …
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We look for students who are passionate, dedicated, energetic, resourceful and creative. We seek students who possess fundamental skills and the desire to launch their career into the future. In this …
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What: 2016 Salute to Achievement gala When: Monday, September 26th, 2016 8am-11pm Location: David Koch Theater 20 Lincoln Center New York, NY 10023 Assistance is needed to assist the Glow Media Team w…
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DTE Energy: Winter weather causes thousands of power outages
(WXYZ) - The wintry mix that hit Metro Detroit is responsible for knocking out power to thousands of DTE Energy customers.
DTE says 22,000 homes and businesses were without electricity on Monday morning due to the weather conditions. There are currently fewer than 1,000 customers without power as of 3:40 p.m. on Monday.
DTE says some of the outages are in Waterford Township where a substation suffered a malfunction. The remainder of the outages are scattered throughout metro Detroit.
DTE says more than 905 of the customers that were affected by bad weather will be restored by the end of Monday. That includes schools.


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Australian actress Radha Mitchell has opened up about how she was asked to take a dip in the mythic but also extremely polluted Ganges as part of her role in a new movie 'The Waiting City'. "There really were pigs running around where we were shooting the scene. And snakes swimming in the water," the […] Continue reading Radha Mitchell's 'horrific' Ganges experience
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The otherwise bold and sexy item girl Koena Mitra turned into a fashionable Radha for fashion designer Joy Mitra's collection showcased at Wills Lifestyle India Fashion Week (WIFW) Friday.
The collection was aimed at depicting the human side of Lord Krishna and had contemporary silhouettes and forms with earthy shades. Koena says she loved walking the […] Continue reading Koena Mitra becomes demure Radha at WIFW


text:
Apoptosis induction in human glioblastoma multiforme T98G cells upon temozolomide and quercetin treatment
- First Online:
- Received:
- Accepted:
DOI: 10.1007/s13277-013-0785-0
- Cite this article as:
- Jakubowicz-Gil, J., Langner, E., Bądziul, D. et al. Tumor Biol. (2013) 34: 2367. doi:10.1007/s13277-013-0785-0
Glioblastoma multiforme is the most aggressive primary brain tumour. At the cellular and molecular levels, several mechanisms responsible for apoptosis or autophagy induction are blocked. Identification of molecular targets stimulating cells to initiate programmed cell death should be performed for therapeutic purposes. A promising solution is the combination of temozolomide and quercetin. The aim of our study was to evaluate the effect of both drugs, applied alone and in combinations, on apoptosis and autophagy induction in human glioblastoma multiforme T98G cells. Our results clearly indicate that quercetin and temozolomide induce apoptosis very significantly, having no effect on autophagy induction. At the molecular level, it was correlated with caspase 3 and 9 activation, cytochrome c release from the mitochondrium and a decrease in the mitochondrial membrane potential. Both drugs are also potent Hsp27 and Hsp72 inhibitors. This suggests that the apoptotic signal goes through an internal pathway. Increased expression of caspase 12 and the presence of several granules in the cytoplasm after temozolomide treatment with or without quercetin preceding appearance of apoptosis may suggest that apoptosis is initiated by ER stress. Additionally, it was accompanied by changes in the nuclear morphology from circular to 'croissant like'.
KeywordsTemozolomide Quercetin Apoptosis Autophagy ER stress Glioblastoma multiforme
Glioblastoma multiforme (grade IV) is the most common and the most aggressive primary brain tumour. It is ultimately recurrent. Despite the enormous progress in the development of surgical techniques and radio- and chemotherapy, treatment of malignant gliomas is extremely difficult and can extend patients' lives by only a few months. The average length of survival of a patient with this type of cancer has not improved significantly over the past few decades remaining at 12–15 months. Only 3–5 % of patients survive more than 3 years. The high invasiveness of glioma results from the capability of cancer cells of extensive proliferation and the 'diffuse infiltration' between normal brain cells, which makes them elusive targets for effective surgical management [1]. The problem of resistance of gliomas to treatment should also be analysed at the molecular level. In glioblastoma cells, several mechanisms responsible for programmed cell death induction like apoptosis or autophagy are blocked, while molecular chaperones promoting cell survival are overexpressed [2–4]. A better understanding of the complex biology of glioma cells is essential for identification of key molecular targets stimulating signalling pathways that lead to initiation of programmed cell death.
Temozolomide is a new drug in glioma therapy, which alone or in combination with radiotherapy prolongs the median survival time. The cytotoxicity of temozolomide is due to formation of O6-methylguanine in DNA which mispairs with thymine during the next cell cycle of DNA replication. In consequence, glioma cells respond by undergoing G2/M arrest and ultimately die by autophagy or apoptosis [5–9]. Our earlier experiments conducted on the anaplastic astrocytoma cell line MOGGCCM revealed that the anticancer properties of temozolomide are potentiated by quercetin (3,3′,4′,5,7-pentahydroxyflavone), a natural flavonoid found in a broad range of fruits and vegetables, e.g. apples, grapes, lemons, onion, kale or tomatoes, with the daily intake of about 30 mg. It has multiple biological, pharmacological and medical applications. It is one of the most potent antioxidants. Quercetin also facilitates apoptosis of tumour cells by activation of caspase 3 and caspase 9 and cytochrome c release. It is also a well-known inhibitor of heat shock protein expression [10–16].
Therefore, the aim of our study was to investigate the effect of temozolomide and quercetin applied alone or in combination on apoptosis and autophagy induction in the human glioblastoma multiforme T98G cell line. We analysed the morphology typical of programmed cell death as well as the molecular mechanism underlying this process based on Hsp27, Hsp72, cytochrome c, beclin 1, caspase 12 protein expression and caspase 3, caspase 8 and caspase 9 activity. Analyses of the shape of the cell nuclei as a hallmark of glioblastoma were also performed.
Materials and methods
Cells and culture conditions
The human glioblastoma multiforme cells (T98G, European Collection of Cell Cultures) were grown in a 3:1 mixture of DMEM and nutrient mixture F-12 Ham (Ham's F-12) (Sigma) supplemented with 10 % FBS (Sigma), penicillin (100 U/ml) (Sigma) and streptomycin (100 μg/ml) (Sigma). The cultures were kept at 37 °C in humidified atmosphere of 95 % air and 5 % CO2.
Drug treatment
Temozolomide (TMZ, Schering-Plough) at the final concentrations of 1, 5, 10, 25, 50, 100, 150 and 200 μM and quercetin (3,3′,4′,5,7-pentahydroksyflavone) (Sigma) at the final concentrations of 1, 5, 10, 25, 50 and 100 μM were used in the experiments. The drugs were dissolved in dimethyl sulfoxide (DMSO, Sigma). The final concentration of DMSO in the culture medium did not exceed 0.01 %, which as indicated in preliminary experiments did not influence cell viability and the expression of the proteins studied. Three variants of drug treatment were performed. In the first one, the cells were incubated only with quercetin or temozolomide for 24, 48 or 72 h. In the second variant, quercetin and temozolomide were added to the culture medium simultaneously and incubated for 24 or 48 h. In the third one, the cells were pre-incubated with one of the two drugs for 6 or 24 h, followed by treatment with the other drug for the next 18 or 24 h. As a control, the cells were incubated with 0.01 % of DMSO.
Detection of apoptosis and necrosis with fluorochromes
For identification of apoptosis and necrosis, the cells were stained with a mixture of fluorescent dyes Hoechst 33342 (Sigma) and propidium iodide (Sigma), respectively [17]. The morphological analysis was performed under a fluorescence microscope (Nikon E-800). Cells exhibiting blue fluorescent nuclei (fragmented and/or with condensed chromatin) were interpreted as apoptotic. Cells exhibiting pink fluorescent nuclei were interpreted as necrotic. At least 1,000 cells in randomly selected microscopic fields were counted under a microscope. Each experiment was performed in triplicate.
Detection of acidic vesicular organelles with acridine orange
Autophagy is a process of sequestrating cytoplasmic proteins into the lytic compartment and is characterised by formation and promotion of acidic vesicular organelles (AVOs). Vital staining with 1 μg/ml of acridine orange (AO) for 15 min was performed for detection of AVOs in the cells treated with quercetin and/or temozolomide [18]. Typical AO-positive cells exhibiting granular discretion of AVOs in the cytoplasm were interpreted as autophagic. A morphological analysis was performed under a fluorescence microscope (Nikon E-800). At least 1,000 cells in randomly selected microscopic fields were counted. Each experiment was performed in triplicate. The percentage of autophagic cells was calculated as the number of cells with AVOs versus the total number of stained cells.
Detection of apoptosis and necrosis by flow cytometry
The Annexin V-FITC apoptosis detection kit (Sigma) was used for detection of apoptosis and necrosis by flow cytometry. The samples were prepared according to the manufacturer's protocol. Briefly, the control and drug-treated cells were incubated with 5 μl of Annexin V-FITC and 10 μl of propidium iodide for 10 min. Immediately after staining, the cells were analysed on the FacsCanto instrument (Becton Dickinson, San Jose, CA, USA). A total of 30,000 events were acquired and analysed using FacsDiva software. Cells which were in the early stage of the apoptotic process were stained only with Annexin V-FITC, whereas late apoptotic cells were stained by both fluorochromes. Cells stained only with propidium iodide were interpreted as necrotic. Live cells showed no staining by either propidium iodide or Annexin V-FITC. Each experiment was performed in triplicate.
Mitochondrial membrane potential
Fluorochrome 3,3′-dihexyloxacarbocyanine iodide DiOC6(3) was used for studying the changes in the mitochondrial membrane potential (∆ψm) in cells incubated with quercetin and temozolomide. At low concentrations, it accumulates in the mitochondria. Loss in DiOC6(3) staining indicates disruption of the mitochondrial inner transmembrane potential [19]. The control and drug-treated cells were incubated with the fluorochrome at the final concentration of 50 nM for 20 min at 37 °C in the dark, washed three times with PBS and subjected to the FacsCanto instrument (Becton Dickinson). A total of 30,000 events were acquired and analysed using FacsDiva software. Each experiment was performed in triplicate.
Isolation of the mitochondrial fraction
A commercial isolation kit was used (Sigma) for the isolation of the mitochondria. The samples were prepared according to the manufacturer's protocol. Briefly, after quercetin or/and temozolomide treatment, the cells at the density about 2 × 107 were trypsinised, centrifuged for 5 min (600 × g) and washed twice with PBS. The cell pellet was resuspended in lysis buffer (Sigma), incubated for 5 min on ice and centrifuged for 10 min (600 × g). The supernatant was transferred onto a fresh tube and centrifuged at 11,000 × g for 10 min. The pellet was resuspended in cell lysis buffer and used for electrophoresis.
Isolation of the cytosolic fraction
After quercetin and/or temozolomide treatment, the cells were lysed in hot SDS-loading buffer (125 mM Tris–HCl pH 6.8; 4 % SDS; 10 % glycerol; 100 mM DTT), boiled in a water bath for 10 min and centrifuged at 10,000 × g for 10 min, and the supernatants were collected. The protein concentration was determined by the Bradford method [20] and samples of the supernatants containing 80 μg of proteins were used for electrophoresis.
Cytoplasmic and mitochondrial samples were separated by 10 % SDS-polyacrylamide gel electrophoresis [21] and subsequently transferred onto Immmobilon P membrane (Sigma). Following the transfer, the membrane was blocked with 3 % low fat milk in PBS for 1 h and incubated overnight with mouse anti-Hsp72 monoclonal antibody (SPA 810, StressGen) diluted 1:1,000, anti-Hsp27 (SPA 800, StressGen) diluted 1:1,000, rabbit beclin 1 antibody (Sigma) diluted 1:500, anti-caspase 12 (Cell Signaling) diluted 1:1,000 and sheep anti-cytochrome c antibody (Sigma) diluted 1:1,000. The membranes were washed three times for 10 min with PBS containing 0.05 % Triton X-100 (Sigma) and incubated for 2 h with a 1:30,000 dilution of alkaline phosphatase-conjugated goat anti-mouse IgG, anti-sheep IgG or anti-rabbit IgG (Sigma). The membranes were visualised with alkaline phosphatase substrate (5-bromo-4-chloro-3-indolylphosphate and nitro-blue tetrazolium, Sigma) in colour development buffer (DMF, Sigma). The data were normalised relative to β-actin (Sigma, working dilution 1:2,000, data not shown).
The levels of protein expression were determined using the Bio-Profil Bio-1D Windows Application V.99.03 programme. Three independent experiments were performed.
Caspase activity assay
Caspases are cysteine proteases, which in normal conditions exist as inactive pro-forms or zymogens. They are cleaved to the active form following induction of apoptosis. The activity of caspases 3, 8 and 9 was estimated using the SensoLyte®AMC Caspase Substrate Sampler Kit (AnaSpec) in the control and drug-treated cells. Sample preparation and enzymatic reaction were performed according to the manufacturer's protocol. The fluorescence of AMC was monitored at Ex/Em = 354 nm/442 nm in 96-well black microplates using 2030 Multilabel Reader VictorTMx4 (Perkin Elmer).
Nuclear morphology
For the analysis of the nuclear shape in the control and drug-treated cells, the roundness factor was used [22, 23] with value 1 for a circle and smaller values for increasing irregularities of nuclear shape. For visualisation of the nucleus, the cells were stained with Hoechst 33342 at a final concentration of 80 μg/ml for 5 min. The measurement of the shape of the nuclei was performed with the Image J programme on digital microscopic images, projected on the computer screen. The tumour cell nuclei were manually traced. A minimum of 300 tumour nuclei per experimental variant were analysed. Three experimental variants were performed.
ER staining
For identification of ER, a staining method with fluorochrome 3,3′-dihexyloxacarbocyanine iodide was used [24]. The cells were incubated with 10 μM of DiOC6(3) for 10 min in the dark at 37 °C. Morphological analysis was performed under a fluorescent microscope Nikon E-800.
Statistical analysis
The data are presented as mean ± standard deviation (SD). The statistical evaluation was performed with a one-way ANOVA test followed by Dunnett's multiple comparison test. P < 0.05 was taken as the criterion of significance.
Sensitivity of T98G cells to temozolomide or quercetin treatment
Similar to temozolomide, quercetin was also effective in apoptosis induction, but in comparison to the alkylating drug, its pro-apoptotic properties were weaker (Fig. 2a–c). Quercetin induced apoptosis very effectively after the 48-h-long treatment with the concentrations of 25 (14 %) and 50 μM (22 %) (Fig. 2b, c). The longer period of incubation with the higher flavonoid concentration induced mainly necrosis (data not shown). The necrotic type of cell death also dominated after the 24-h-long incubation with the concentration above 100 μM (Fig. 2a). Similar to TMZ, quercetin had no significant effect on autophagy induction.
Sensitivity of T98G cells to combined temozolomide and quercetin treatment
When the T98G cells were treated with 100 μM of TMZ combined with different quercetin concentrations for 24 h, the number of apoptotic cells was higher than that observed after incubation with 50 μM of the alkylating drug (Fig. 3g–i). Application of 100 μM TMZ and 50 μM of quercetin induced apoptosis in 27 % of cells; unfortunately, it was accompanied by a significant necrosis, and after 48 h of incubation, necrosis was dominant (data not shown). Therefore, temozolomide and quercetin at the concentration of 50 μM applied to the culture medium for 48 h were chosen for further experiments. Considering the precedence of drug addition, the simultaneous treatment and pre-incubation variant with temozolomide were the most effective. Pre-incubation of cells with quercetin followed by the TMZ treatment was less effective. Incubation with both drugs had no effect on autophagy induction.
Evaluation of cell death and the mitochondrial transmembrane potential by flow cytometry
To confirm the reliability of the results obtained from the microscopic observations, the T98G cells treated with quercetin (50 μM) and temozolomide (50 μM) separately as well as in combination of both drugs for 48 h were analysed by flow cytometry according to the type of cell death (apoptosis or necrosis) and mitochondrial membrane potential.
The effect of 48-h-long incubation of T98G cells with quercetin (Q, 50 μM) and/or temozolomide (T, 50 μM) on apoptosis and necrosis induction
Necrosis (%)
Early apoptosis (%)
Late apoptosis (%)
0.25 ± 0.07
0.75 ± 0.2
0.14 ± 0.1
2.7 ± 0.4
8.18 ± 1.2*
8.56 ± 0.07*
0.5 ± 0.14
3.82 ± 0.14
9.15 ± 0.2*
0.8 ± 0.07
9.95 ± 0.49*
8.2 ± 0.42*
0.8 ± 0.0
11.75 ± 0.07*
11 ± 1*
0.8 ± 0.14
11.26 ± 0.77*
10.3 ± 0.6*
The effect of temozolomide and quercetin on the expression of marker proteins
The quantitative and qualitative analyses of the immunoblots revealed that quercetin and temozolomide inhibited Hsp72 and Hsp27 expression in all the experimental variants. The studied drugs applied alone or in combinations decreased the level of cytochrome c in the mitochondrial fraction, which was accompanied by increased accumulation of the protein in the cytoplasm. Quercetin and temozolomide had no significant effect on the expression of the autophagy marker beclin 1 as its level was similar to the control one in all the experimental variants. In the case of caspases, increased expression of caspase 12 was observed after treatment with temozolomide separately as well as in combination with quercetin. Both drugs applied alone or in combination significantly increased the activity of caspase 3 and caspase 9. The simultaneous drug treatment was the most potent. TMZ applied separately as well as in combination with quercetin did not change the caspase 8 activity. Its inhibition was observed after the quercetin treatment.
The effect of temozolomide and quercetin on the shape of nuclei and cytoplasm organisation
Localisation of Hsp27 and Hsp72 in T98G cells
Most strategies for high grade gliomas are aimed to maximise PCD type I apoptosis or type II autophagy. These are predominantly distinctive processes, but many studies suggest a cross-talk between the two. Apoptosis may occur via death receptor-dependent (extrinsic, cytoplasmic) and receptor-independent (intrinsic, mitochondrial) pathways. The mitochondrial pathway is mediated by Bcl2 family proteins, which regulate disruption of the mitochondrial membrane and release of cytochrome c to the cytosol and lead to the activation of caspases and cell death in consequence. The extrinsic pathway is triggered through the activation of death receptors and involves caspase 8 maturation. Both pathways converge to a final common pathway correlated with activation of caspase 3 [4]. Unlike apoptosis, which is a caspase-dependent process, autophagy is a caspase-independent programmed cell death, characterised by accumulation of autophagic vacuoles (AVOs) in the cytoplasm accompanied by degradation of Golgi apparatus and ER, which precedes the destruction of the nucleus. Autophagy is suppressed during the early stages of tumour progression. Therapeutically, increased autophagy could represent an alternative way to destroy cancer cells. It would be beneficial in the light of recent investigations, as gliomas naturally resist apoptosis [5, 25]. On the other hand, many studies provide data suggesting that autophagy represents a protective mechanism helping cancer cells to get rid of toxic particles and increase the survival in consequence. In the light of these observations, redirecting cancer cells to enter the apoptotic pathway would be beneficial [26, 27]. Our earlier experiments conducted on the MOGGCCM anaplastic astrocytoma cell line revealed that temozolomide induced autophagy more efficiently than apoptosis [15, 16]. We demonstrated for the first time that combinations of TMZ with quercetin were much more effective in programmed cell death induction in glioma cells. At a low (5 μM) drug concentration, quercetin potentiated the pro-autophagic effect of temozolomide, while after treatment with a higher drug concentration (30 μM), autophagy switched to apoptosis. Therefore, we decided to evaluate the effect of temozolomide applied alone or in combination with quercetin on induction of apoptosis and autophagy in the human glioblastoma multiforme T98G cell line. Our results indicated that temozolomide and quercetin were very effective apoptosis inducers in the studied cells. The combination of both drugs was even more effective. At the molecular level, it was correlated with the decreased mitochondrial membrane potential, release of cytochrome c from the mitochondrium to the cytoplasm and activation of caspase 3 and caspase 9. No activation of caspase 8 was observed. All these observations suggest that both drugs initiate apoptosis via the internal (mitochondrial) pathway. In contrast to the MOGGCCM cell line, neither temozolomide alone nor its in combination with quercetin had an effect on autophagy induction in the T98G cells. This was confirmed by the unchanged level of autophagy marker beclin 1 in comparison to the control. This is a critical component involved in autophagosome formation at the early stage of autophagy, which at the basal level of expression is not able to initiate the process of death [28]. It has also been shown that activation of caspase 3 inhibits autophagosome formation and may block beclin 1 activity or even cleave the protein [29]. Furthermore, the expression of beclin 1 is reduced during gliomagenesis, being progressively lower through grade I to grade IV gliomas [30].
Staining the cells with Hoechst 33342 for visualisation of signs of apoptosis additionally revealed that temozolomide alone and together with quercetin changed the morphology of glioblastoma nuclei from circular, observed in the control, to more curved, or 'croissant like'. It is known that tumour cell nuclei belong to the most important histological structures in the assessment of tumour biology including typing and grading of gliomas. Histomorphometry of tumour cell nuclei could also be a promising approach for estimation of the prognosis for individual patients. It was shown that the nuclei had a tendency toward a more circular shape in short survivors [22, 23].
The microscopic observation also revealed small round granules in the cytoplasm after temozolomide treatment, both alone and in combination with quercetin. They were not stained with acridine orange—a fluorochrome typical in the detection of autophagosomes. Staining of cells with DiOC6(3), a dye that recognises ER within cells, revealed the presence of rounded structures in the reticulum. They were visibly surrounded by Hsp27. This was accompanied by increased caspase 12 expression. All those observations may suggest that this might be correlated with the ER stress response. As tumours increase in size, cells are exposed to several environmental stressors like hypoxia, limited nutrients and acidosis. This leads to accumulation of misfolded and aggregated proteins in the cytoplasm. Such perturbations in protein folding are also observed after exposure to chemotherapy. When molecular chaperones cannot keep up with denaturated and misfolded proteins, they aggregate in larger, uniform in size, membrane free aggresomes present throughout the cytoplasm, leading to ER stress. In response to this, the unfolded protein response is activated. It initially tries to restore normal ER homeostasis by activation of the proteasome complex or autophagy, when aggresomes fuse with lysosome and create autophagolysosomes. In this context, autophagy is a pro-survival process [31]. If damage is too severe, the death pathways are activated especially through the mitochondrial apoptotic pathway [32]. The precise molecular mechanisms of ER stress-induced apoptosis, however, have not been fully elucidated. It is known that ER stress leads to caspase 12 activation [33]. Processed caspase 12 reportedly activates caspase 9, which is followed by activation of caspase 3 [34]. On the other hand, some reports demonstrate that in fact the mitochondrial pathway of apoptosis plays a significant role in ER stress-induced death, while activation of caspase 12 plays a minor role in the process [35]. In our experiments, the presence of granules and a cleaved form of caspase 12 followed by apoptosis induction was shown in the cells treated with temozolomide alone or with quercetin. In the cells incubated with quercetin, we noticed neither granules nor cleavage of caspase 12, which may suggest that the ER stress-activated apoptosis in glioma cells after temozolomide treatment is correlated with activation of caspase 12. The lack of ER stress signs in cells incubated with quercetin may be explained by the fact that the antioxidant protects cells from ER stress-induced cell death [36].
Hsps are molecular chaperones whose enhanced expression inhibits ER stress, induces apoptosis and leads to cell survival [32]. The mechanism of Hsp27 and Hsp72 apoptosis blockade is complex. They prevent cytochrome c release, mitochondrial membrane permeabilisation, recruitment of procaspase 9 to the apoptosome and its subsequent maturation to the active form. Hsp72 migrates to the nucleus to protect nuclear proteins from caspase 3 cleavage. Hsp27 may bind to F-actin, thereby preventing cytoskeleton disruption. It has also been observed that Hsps may be sometimes fused to aggresomes, but they are not directly engaged in misfolded protein degradation. They only recognise them. Depletion of Hsp27 and Hsp72 severely decreases degradation of misfolded proteins and decreases cell survival [31, 32, 37, 38]. All those protective effects of Hsps are beneficial in normal cells. In the case of cancer cells where the level of Hsps is abnormally high, these molecular chaperones act as negative prognostic markers. It was also observed that in many cases, the expression of the proteins rose with the grade of the tumour. It has even been suggested that heat shock proteins may participate in oncogenesis. Therefore, inhibition of Hsps expression has become a novel strategy for cancer therapy [39–41]. In our experiments, both temozolomide and quercetin partially inhibited Hsps expression in T98G cells.
In summary, our results indicate that temozolomide and quercetin are very potent apoptotic but not autophagic inducers in glioma multiforme T98G cells. It seems that the apoptotic process is preceded by ER stress followed by activation of the mitochondrial death pathway. Our observations may form a basis for further studies on using this drug combination in clinical application.
Conflicts of interest
Copyright information
Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.


text:
Rocket Attack U.S.A. (1961)
Plot Summary
A cold-war propaganda film, released not too long after the launch of Sputnik, intended to rally public support for an anti-ballistic missile program. Based on the assumption that Sputnik is gathering intelligence for the Soviets, a spy is sent to Moscow to determine just how far along their missile program is. Surprise, surprise; Sputnik gave them all the data they need to build one nuclear missile, which is ready to launch against the U.S.- Written by Leo L. Schwab <email@example.com>
An American secret agent tries to stop the Soviets from launching an intercontinental missile at the mainland United States.- Written by Shannon Patrick Sullivan <firstname.lastname@example.com>
Be the first to contribute! Just click the "Edit page" button at the bottom of the page or learn more in the Synopsis submission guide.


text:
Quick N Easy Grits and Veggie Omelet
Total Time
Prep 15 mins
Cook 5 mins
Low Fat, Low Sodium, High on Flavor!
Ingredients Nutrition
- 1⁄4 cup quick-cooking grits, NOT instant
- 1 cup water
- 1 egg white
- 1 tablespoon bell pepper, chopped
- 1 tablespoon celery, chopped
- 1 tablespoon mushroom, chopped
- 1 tablespoon onion, chopped
- 1 tablespoon fresh ground black pepper
- 1 tablespoon Mrs. Dash seasoning mix, Original Blend
- Chop vegetables. Plan ahead and stock a few plastic containers in your fridge with a supply of chopped veggies you use frequently. When you need them they are ready.
- Break egg in a bowl, use a strainer spoon to remove yolk.
- Mix all ingredients in a microwave-safe bowl.
- Cook in microwave on High for about 4 to 5 minutes.
- Serve with a few 1/2 slice of turkey bacon and a few slices of fresh fruit.


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Basilicata, Rock Hard And Still Untamed
Published: September 3, 2000
(Page 3 of 4)
Rionero, on the bottom slopes of Mount Vulture, a spent volcano, is the region's prime wine-growing area. The excellent local wine -- made with the aglianico vines imported some 2,700 years ago by the settlers of Magna Graecia -- has had a DOC, an official wine denomination, since 1971.
The aglianico del Vulture red is rich and clean, good company for the stewed wild cardoncelli mushrooms, the creamy fave e cicoria (bean puree with greens), the home-made scorze di mandorla and cavatelli pasta, the grilled sausages flavored with fennel, and the many interesting sheep and cow cheeses found here. These include the rare caciocavallo podolico, made from the exotically flavored milk of a cow that grazes only the highest pastures, chewing up rare grasses and wildflowers, tender mushroom caps or rucola muralis, the tiny-leafed spicy wild rocket. (We lunched on slivers of that wonderfully spicy, crumbly yellow cheese from Latteria Rizzi, a cheese shop in Matera.)
We hike partway up Mount Vulture hoping to catch a glimpse of some of those high-grazing cows, but no luck. What we can just see are the pretty Monticchio lakes nearby, where, Horace tells us, he once ran away from his wet nurse as an infant and got lost in the woods. Fortunately -- befitting a baby who was going to become a famous poet -- magical doves came and covered him with bay and myrtle leaves, so that the bears and vipers wouldn't harm him.
Heading back toward Potenza and Naples, we make a last stop at Castel Lagopesole, a big square reddish castle built in 1242 by Frederick II of Swabia (or of Hohenstaufen), who ruled southern Italy. It dominates the heights so absolutely that a chicken couldn't have crossed the road in the valley below without the Hohenstaufens knowing about it.
The sun is hot and a cuckoo begins to hoot, loud as a foghorn. If you think of it in premodern terms, says Vittorio, who likes to read history, Frederick was probably the most tolerant and enlightened signore who ever ruled these parts. What would Basilicata be like today if his line had survived and the corrupt, absentee Angevins, Spanish and Bourbons had never gained control?
Much more prosperous, but possibly not so wild and beautiful, I hazard.
''Hmm,'' Vittorio says. ''Yes, that's probably right.''
A forgotten corner of Italy, full of surprises
Getting There
Matera is 170 miles east of Naples on the A3 Autostrada to Potenza, then Strada Statale 407. There are flights to Naples and Bari, which is about 40 miles from Matera. Renting a car is advisable; train service in Basilicata is very slow.
Although the summer heat is not unbearable, April through June and September through December are the most pleasant months to visit.
For information on Basilicata, contact the APT (tourist office) in Matera, (39-0835) 331-983, or in Potenza, (39-0971) 411-839. Carlo Levi's 1945 book ''Christ Stopped at Eboli'' (Noonday Press, $13) remains a wonderful guide to the region.
Where to Stay
Don't expect luxury hotels in Basilicata, although all of these offer a telephone, TV and minibar.
Grande Albergo, Corso XVIII Agosto 46, Potenza 85100, telephone and fax (39-0971) 410-220, is Potenza's most modern hotel. The 70 rooms offer spectacular views of the valley below. A double with bath, including breakfast, costs about $85, at the rate of 2,150 lire to the dollar.
Hostel I Sassi, Via San Giovanni Vecchio 89, Matera 75100; (39-0835) 331-009, fax (39-0835) 333-733. ''Hostel'' suggests bunk beds and shared bathrooms, but there are no privations at this delightful place built right into the sassi, with superlative views. Our cool, white room, on two levels, had a large private terrace and a funky bathroom carved out of the rock. The bar, breakfast room and 14 other guest rooms are also in converted caves (four rooms have air-conditioning). Doubles with bath are $65, breakfast included.
Albergo Italia, Via Ridola 5, Matera 75100; (39-0835) 333-561, fax (39-0835) 330-087. This elegant, old-fashioned hotel in the old city has nice views of the sassi. All rooms are air-conditioned and some have antiques. Doubles are $85, with breakfast.
Hotel Due Pini, Piazzale Stazione, Melfi, 85025, (39-0972) 21031, fax (39-0972) 21608, is a simple 48-room hotel, offering excellent value right downtown. Doubles with bath start at $50, breakfast included.
Hotel La Pergola, Via L. Lavista 27-31, Rionero in Vulture, (39-0972) 721-179, fax (39-0972) 721-819, is well situated for exploring Mount Vulture. This modest, comfortable hotel has 43 rooms and a large, cheery restaurant with a good local wine list. Doubles with bath cost $55, including breakfast.
Where to Eat
Simple, rustic and based on the freshest of ingredients, the delicious food of Basilicata is what the shepherds might have eaten in Arcadia. Specialties include grilled meat and homemade sausages, wild mushrooms and field greens, fresh sheep- and cow's-milk cheeses, and distinctive wheat pastas like manate, cavatelli and strascinati, shaped roughly like little caps or boats.


text:
Not Available
Bassinet & Mattress Pad Set
- Overview
Keep your newborn nearby in this beautifully constructed bassinet, made extra cozy with the braided detail.
- 21.75" wide x 37.75" long x 39" high
- Bassinet is tightly woven of rattan that's been sealed, with wood detailing and a cross-braced platform made of mahogany for stability.
- Top can be removed to create a separate sleep basket, which can easily be transported anywhere in the house.
- Bottom shelf provides extra storage.
- Rolls on locking casters and includes waterproof mattress pad.
- Rigorously tested to meet or exceed all required and voluntary safety standards enacted by the US Product Safety Commission, the American Society of Testing Manufacturers and the Canadian Cribs and Cradles Regulations. See our crib safety tips
- Dimensions & Care
- Overall: 21.75" wide x 37.75" long x 39" high
- Basket Only: 21.5" wide x 37.75" long x 20.75" high
- Frame Only: 21.25" wide x 36.25" long x 22.75" high
- Basket Interior: 14.25" wide x 29" long x 10.5" high
- Shelf: 18" wide x 20" long
- Clearance Above Shelf: 9.75" high
- Shelf Top to Floor: 7.75" high
- Distance Between Casters and Legs: 20.5" (short side), 20.75" (long side)
- Casters: 2" diameter, 2.5" high
- Weight Capacity: 30 pounds
- Weight: 16 pounds
- Dust often using a soft, dry cloth.
- Protect from direct sunlight and extreme temperatures.
- Blot spills immediately with a clean, damp cloth.
- We do not recommend the use of chemical cleansers, abrasives, furniture polish or wax.
- Shipping & Return Info
This is a general shipping item.
View Our Shipping Information
Return Policy
At Pottery Barn Kids, we take great pride in the quality and craftsmanship of our products. Attention to design, materials, safety and construction is our priority. We carefully inspect your order prior to shipment; upon receipt, please inspect your purchase and notify us of any damage. We will arrange for a prompt replacement. If, within 7 days for Quick Ship upholstery and 30 days for all other products, you are dissatisfied for any reason, you may return your purchase for a refund of the merchandise value.
If your return for Merchandise Credit Cards is greater than $10,000, we will issue $10,000 in Merchandise Credit Cards to you that day, and then mail additional Merchandise Credit Card(s) for the amount in excess of $10,000 to you.
We cannot accept returns on monogrammed, personalized, special-order items, or on items damaged through normal wear and tear. We also cannot accept returns of final-sale items, which are identified by a price that ends in $0.99.
For returns of items purchased from your Pottery Barn Kids registry, we will gladly provide a refund or exchange for the merchandise within 90 days of your event or within 90 days of purchase, whichever date is later.
For assistance, please contact Customer Service at 800.993.4923, 7 days a week, 5:00 a.m. - 9:00 p.m. PST.
International Returns: Online returns are available only for items that were shipped to the United States. If you would like to return items that were shipped outside the US, please see International Returns and Exceptions.


text:
A 2009 graduate of the respiratory care program and currently working at Hamot Medical Center, Daisha Hansbrew is seen here working with one of the high fidelity mannequins in the SIM center. This section of the SIM is most often used to stage scenarios in the care of patients in Emergency Departments, ICU or the OR. The mannequins are moulaged, or have make-up applied to represent patient situations such as multiple trauma, cardiac arrest or surgery.
Along with 11 adult mannequins, the SIM center houses one newborn, one baby and one child mannequin. Notice the blue color around the baby's nose and mouth representing respiratory difficulty! Students are able to listen to this baby's lung sounds, which indicate severe breathing problems.
The state-of-the-art control room of the SIM allows faculty to manipulate patient parameters and video record student activity. After the scenario is run, students and faculty participate in a debriefing period that includes reviewing the tapes and critiquing patient care efforts. Many trauma centers use this method of evaluation of patient care to enhance patient care delivery.
2009 graduate Daisha Hansbrew works in the SIM center with a critically ill "patient" receiving blood, continuous hemodynamic monitoring and state of the art mechanical ventilation. The student is able to listen to this patient's breath, heart and bowel sounds as well as palpate their pulses.
Assistant Professors Charles Cornfield, M.S. and Valerie Baker, APRN, BC lead multidisciplinary clinical scenarios with students from three of Gannon University's eight health science majors. Students are afforded the opportunity to work as a team in the care of complex patient scenarios presented in the Patient Simulation Center.
Assistant Professor Charles Cornfield, M.S. (far right) leads a critical care scenario with (from l to r): Michael Eller, a 2009 Physician Assistant graduate currently working at Great Lakes Family Medicine; Nicolette Critelli, a 5th year Physician Assistant student in the Five-Year PA Program; Crystal Wozniak, a 2009 PA graduate, currently working at Great Lake Surgical Associates/Hamot Shock-Trauma; and nursing student Kristen Baker.
Professor Gary Berringer, MSN, RNC (center), a certified neonatal nurse, coaches Crystal Wozniak (l), a 2009 PA graduate currently working at Great Lake Surgical Associates/Hamot Shock-Trauma, and Christa Blose (r), a 2009 PA graduate currently working at Metabolic Disease Associates of Erie, through a scenario involving a high risk neonate. The SIM center houses a mannequin that gives birth (and speaks several languages), allowing faculty to program the mannequin so various complications associated with delivery are experienced by health science students.
In the multipurpose simulation lab, 2009 PA graduate Michael Eller (l) and senior respiratory care students Robert Anderson (center), and Kathleen Combopiano (r) work with a mannequin to practice physical assessment skills such as auscultation of cardiac and breath sounds.
Assistant Professor Valerie Baker, APRN, BC demonstrates use of one of the Simulation Center's two virtual IV computer training systems. The realistic "arm" of the system allows students the opportunity to critically think through a patient situation, select the appropriate type of IV needed, palpate the vein (which will be cannulated) and "virtually" start an IV. The system also provides students with an evaluation of their performance and suggestions for improvement.
The Patient Simulation Center also contains a 40 seat classroom where a Wolf Camera located in the ceiling provides a close-up view of the demonstration of skills, such as suturing, for all in the classroom to see.


text:
I have a client who wants to split up his articles to feature an ad inbetween at a certain point. is there a way to do this with "the_content" ?
I have a client who wants to split up his articles to feature an ad inbetween at a certain point. is there a way to do this with "the_content" ?
...wants to split up his articles to feature an ad inbetween at a certain point.
what is a 'certain point' in programming terms?
to put the ad into single post at the position of the more-tag (if it does not collide with the shortform of the post in archives or on the home page), you could try and follow this article:
(adaptable to non-adsense ads)
create a shortcode to place the ad; does not have to be as complicated as this:
(adaptable to non-adsense ads)
build a filter function for 'the_content' to insert the ads after a certain number of paragraphs or words.
search the http://wordpress.org/extend/plugins/search.php?q=advertising&sort=
What if this is not an ad. What if I want to split up the content and put a bar in between the top portion and bottom portion of the content with News, links, videos, etc.?
Horizontal Bar (at 100%) with important info.
rest of the content.
Is this possible with pages and posts?
Yeah, the concept is the same. It's about breaking up the content, what you insert after doing that is inconsequential
Can you give me more detail? I am fairly new and the above links are complicated for me.
Can you give me more detail?
for more detail, we need more details first:
can you post the name of your theme and a link to your site, and more information what you like to insert where into the content of posts and pages?
is it always a fixed extra content, or depending on something ?
using a filter function for 'the_content' seem to be one possible way to go; either in the 'read-more' position or after a set amount of paragraphs or words;
alternatively you could try and use a shortcode so the user has control if and where the content will show.
The site is http://flagstaffit.com/azra
Our dreamhost server went down the other day so it is really slow right now and may not come up. Hopefully you can see it. I have just begun designing the theme. There is no conent
The red bar across the bottom is supposed to hold miscellaneous widgets and eventually will be cutting the middle of the content.
I read the codex page on "the_content" but think it may be above my level of understanding. Maybe you can explain how to do the shortcode?
The top portion of content is always the same height so maybe I can guess on the number of paragraphs, but then it will be messed up if they put in a video or image. So maybe the short code is the best answer?
'shortcode' is out of the discussion, it is not drastic enough to match your design idea.
this question is a near duplicate to: http://wordpress.org/support/topic/need-help-on-how-to-design-a-site-with-a-static-bar-through-middle-of-content?replies=4
I suggest to focus on that one.
This topic has been closed to new replies.


text:
Taste of Brevard begins on Tuesday
Published: Saturday, September 23, 2006 at 4:30 a.m.
Last Modified: Saturday, September 23, 2006 at 12:00 a.m.
The Fourth Taste of Brevard will be held from 7 to 9 p.m. Tuesday in Thomas Hall at the Brevard Music Center. The event benefits Communities in Schools of Transylvania County.
The event features samplings from restaurants and a silent auction. The cost is $15 for adults and $8 for children under 12. For more information, call 885-7390.
Reader comments posted to this article may be published in our print edition. All rights reserved. This copyrighted material may not be re-published without permission. Links are encouraged.
Comments are currently unavailable on this article


text:
Secondary menu
Brooklyn Beckham and Elizabeth Hurley join couple in sharing wedding photographs on social media
Meanwhile, Guardian's rivals round on the paper for supporting the press charter Grant wants
Phone-hacking victims dismayed that PM appears to prefer newspapers' own plan for regulator
'I lost myself in Liz Hurley,' says Hollywood actor Tom Sizemore whose memoir details three-year fling
Did Cameron blink first - or did he stand his ground? Either way, he's accepted a 'tiny bit of statute'
PM runs out of patience over 'unbridgeable gap' on press regs. Has he made an historic mistake?
Even its silliest elements are 'guilty pleasures' say critics as Cloud Atlas comes to UK cinemas
Campaign group says its bill is true to the judge's original report and will stop political meddling
British actor 'was rude to programme staff and will never be allowed back on The Daily Show'
Charlotte Church and Gerry McCann mooted as candidates to stand against Cameron, Gove & Co
Steve Coogan, Hugh Grant and Gerry McCann on the attack over crucial need for statutory underpinning
PM and his deputy could make separate statements to Commons, so divided are they on the issue
Media barons enjoy freedom to manipulate opinions of millions, and must be accountable, says Will Hutton
Paper accuses 'liberal Establishment' of putting freedom of speech under threat
Critics say new film is a 'bloated, self-indulgent slog' - but maybe they just don't 'get it'
Media mogul also rounds on 'scumbag' celebrities and castigates the BBC ahead of crucial AGM
Singer holds talks with PM over media regulation and is 'comforted' by outcome
From the Sunday papers: from Cole's reaction to US X Factor sack to Hugh Grant's discourteous performance at ethics debate
Standing ovation for adaptation at Toronto Film Festival but some critics are left with a headache


text:
Official Press Release
Studio 407 announced today that their sci-fi horror property Night and Fog has been optioned for film by renown producer Gil Adler and Shane McCarthy. No stranger to comic book material, Adler has produced such comic book films as âConstantine,â âSuperman Returns,â as well most recently âDead of Nightâ which was based on the popular Italian comic book Dylan Dog.
Said Adler: âThis material is definitely in my strike zone in more ways than one. And having produced the âTales from the Cryptâ series and some Dark Castle horror films, I can say horrorâs a genre I know intrinsically. But what really appealed to me wasnât so much the genre trappings, but rather the characters that really drive this story.â
Tied to an unsolved mystery from World War 2, Night and Fog, tells the story of a âFrankensteinâ like infectious mist unleashed on a military base that transforms its victims into preternatural creatures of the night. But when the survivors try to kill them, they adapt and change into something even more horrific and unstoppable. Caught in between, is a security officer on the base who must escape this gauntlet of horror to save his children before the creatures kill them or the fog infects them.
Added Adler: âWhen I read this I knew I had to take it off the market. Itâs a great high-concept that blends the gothic horror of the Hammer films with the sci-fi horror of âAliensâ and âThe Thing.â
âWeâre really excited to see Gilâs take on the story,â remarked Studio 407 Managing director Alex Leung. âWeâre also honored to be working with an experienced guy like Gil, who knows how to play up iconic figures and can tie them into a horror setting. Heâs the perfect person to bring Night and Fog to lifeâ.
Adler and McCarthy are also working together to produce the adaptation of âHavana Nocturneâ along with Eric Eisner, and recently optioned Ken Bruenâs crime thriller âTower.â Leung will also serve as a producer on the film, having worked on Jackie Chanâs âAround the World in 80 Daysâ as an associate producer, and most recently executive produced the horror film âHunterâ with Stallion Media (âPunisher: WarZoneâ).
Night and Fog is currently available in comic shops in the single issue format and digitally on I-Phone through Comixology. It will be hitting stores in a trade format April/May 2010.
To see more of the products from Studio 407 you can visit www.studio-407.com. Retailers should email firstname.lastname@example.com to get the release information mailing list.
About Studio 407: Studio 407 brings together the imagination and creative talents of writers and artists from North America and Asia to generate a flow of distinct and kinetic East-meets-West entertainment. A writer driven studio that blends innovation with tradition, Studio 407 is dedicated to publishing the highest quality in comics and manga across a wide variety of genres. From capes to kung fu, giant robots to vampires, and secret agents to mad scientists; at Studio 407, we sweat the details. www.Studio-407.com


text:
Skip to comments.Statement by Governor Dean on the California Recall ("It's All About Bush, whiiiiine!")
Posted on 10/07/2003 8:46:28 PM PDT by Timesink
Posted by Mathew Gross at 11:18 PM
FreeRepublic , LLC
It is in the breaking news sidebar!
I thought she voted for Grey Davis
Only a mind-numbed, Kool-Ade drinking democrat would consider a constitutionally legal recall, with petitions signed by upwards of a million voters a "ridiculous power-grab."
Just you wait. Tommorrow the voters will really get even by smashing their faces into your fists and crunching your knee with their groins. Then you will see!
with apology to W.A.
It won't. The anger in California was caused by events specific to California, especially Gray Davis's actions.
There. That's better.
Ah, a SUBTLE Rat troll, there's a rarity. Thanks for the info.
Wait till the dems find out just how right they are!!!! LANDSLIDE BUSH/CHENEY 2004
Exactly, my OldFriend !! ...
November, 2004:
President Bush on the Road to Victory ...
The Democrats are looking for a 1994 repeat and cannot understand that what was rejected in both elections were socialists, who happened to be the 'establishment' at the time.
That will teach us Republicans a lesson!
Maybe that is the new Democrat plan, get democrats thrown out of office, and elect Republicans and then wait for the next election to really teach us a lesson!
Disclaimer: Opinions posted on Free Republic are those of the individual posters and do not necessarily represent the opinion of Free Republic or its management. All materials posted herein are protected by copyright law and the exemption for fair use of copyrighted works.


text:
ODT Online
ODT /directory
The 30-year-old first received the honour back in 2005 and
becomes the only player other than his captain Richie McCaw
to have received the prestigious award more than once.
Carter edged out McCaw, England number 10 Owen Farrell and
France first-five Frederic Michalak for the award in one of
the most closely fought battles in a year of high-calibre
performances on the international stage.
The accolade completes a hat-trick of awards for New Zealand,
as the All Blacks were named IRB Team of the Year for the
sixth time and Steve Hansen the IRB Coach of the Year at the
Rugby World Cup 2015 Pool Allocation Draw in London today.
Carter, the leading point scorer in Test Rugby with 1385 in
his 94 appearances to date, has bounced back in great style
from the disappointment of missing the majority of the All
Blacks' Rugby World Cup-winning campaign through a groin
Last month New Zealand celebrated their third anniversary as
the number one team in the IRB World Rankings, playing an
up-tempo brand of Rugby that was not only pleasing on the eye
but extremely successful.
After an eight-year apprenticeship as Graham Henry's
assistant, Hansen has seamlessly stepped up to the role of
head coach and arguably taken the All Blacks on to even
greater heights.
Under his command New Zealand were unbeaten in the calendar
year until a 38-21 loss to England at Twickenham on Sunday, a
result which brought to an end their 20-match unbeaten run.
In this time the All Blacks claimed the Bledisloe Cup for the
10th consecutive year on their way to the inaugural Rugby
Championship title.
IRB Chairman Bernard Lapasset presented McCaw with the Team
Award and the Players' Award on Carter's behalf, while IRB
Vice Chairman Oregan Hoskins presented Hansen with the Coach
"New Zealand have again been the stand out team of the year
with their 20-match unbeaten run and thoroughly deserve the
IRB Team of the Year award along with Steve Hansen as IRB
Coach of the Year for his feats in keeping a Rugby World Cup
winning-team winning," said Lapasset.
"Dan Carter has bounced back from the disappointment of
missing the majority of the World Cup through injury to show
what a truly magnificent player he is and a wonderful
ambassador for our sport. Winning this award for the second
time reflects his sheer talent and ability."
The IRB Player of the Year award is selected by the IRB
Awards independent panel of judges, comprising former
internationals with more than 500 Test caps between them.
Will Greenwood, Gavin Hastings, Raphaël Ibanez, Francois
Pienaar, Agustín Pichot, Scott Quinnell, Tana Umaga, Paul
Wallace and convenor John Eales watched more than 100 hours
of Test action, awarding points to the three players they
thought stood out in each match.
Previous winners of IRB Player of the Year Award:
2011 - Thierry Dusautoir (France)
2010 - Richie McCaw (New Zealand)
2009 - Richie McCaw (New Zealand)
2008 - Shane Williams (Wales)
2007 - Bryan Habana (South Africa)
2006 - Richie McCaw (New Zealand)
2005 - Dan Carter (New Zealand)
2004 - Schalk Burger (South Africa)
2003 - Jonny Wilkinson (England)
2002 - Fabien Galthié (France)
2001 - Keith Wood (Ireland)
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Times South Canterbury


text:
WICHITA, Kan. (AP) _ Several businesses and residents are suing Coffeyville refinery in federal court seeking more than $4.39 million in damages caused by an oil slick during the July 2007 flood.
The lawsuit claims that when employees left the refinery as flood waters rose they failed to close a valve, allowing more than 90,000 gallons of crude oil, diesel and other pollutants to spill out.
The plaintiffs say the Verdigris River carried a sheen of oil extending 10 miles down the river into Oklahoma. They claim that in addition to damage to homes, businesses, livestock or crops, the contamination also caused loss of income and other expenses.
Coffeyville Resources Refining and Marketing says in a statement that the lawsuit claims are among the few that it has been unable to settle. The refinery says the claims are best resolved in Coffeyville, not federal court.
(Copyright 2008 by The Associated Press. All Rights Reserved.)