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Mitochondrial morphology is maintained by two distinct membrane events -fission and fusion. Altering these conserved processes can disrupt mitochondrial morphology and distribution, thereby disrupting the organelle's functionality and impeding cellular function. In higher eukaryotes, these processes are mediated by a family of dynamin-related proteins (DRP's). In the lower eukaryotes, for instance Dictyostelium discoideum, mitochondrial fission and fusion have been implicated but not yet established. To understand the overall mechanism of these dynamics across organisms, we developed an assay to identify fission and fusion events in Dictyostelium and to assess the involvement of the mitochondrial proteins, MidA, CluA, and two DRP's, DymA and DymB.
Using laser scanning confocal microscopy we show, for the first time, that lower eukaryotes mediate mitochondrial fission and fusion. In Dictyostelium, these processes are balanced, occurring approximately 1 event/minute. Quantification of the rates in midA-, cluA-, dymA-, or dymB- strains established that MidA appears to play an indirect role in the regulation of fission and fusion, while the DRP's are not essential for these processes. Rates of fission and fusion were significantly reduced in cluA-cells, indicating that CluA is necessary for maintaining both fission and fusion.
We have successfully demonstrated that Dictyostelium mitochondria undergo the dynamic processes of fission and fusion. The classical mediators of membrane dynamics - the DRP's – are not necessary for these dynamics, whereas CluA is necessary for both processes. This work contributes to our overall understanding of mitochondrial dynamics and ultimately will provide additional insight into mitochondrial disease.
Mitochondria are cellular organelles that produce energy in the form of ATP, play a central role in cellular metabolic pathways, and are essential for regulating apoptosis [1-3]. In most organisms, mitochondria are reticular, highly branched, complex structures [4]. The maintenance of this structure is vital for the success of these and other essential cellular functions. In both yeast and mammalian cells, this tubular structure is maintained by a balance of two dynamic and highly conserved processes: fission and fusion. Fission is the process by which mitochondria divide, while fusion occurs when neighbouring organelles join together to become one [1,4,5].
Disrupting these processes alters not only the mitochondrial morphology but also the distribution of mitochondria throughout the cell. Uneven distribution decreases the targeting efficiency of critical metabolites, such as ATP [6]. It has also been suggested that fission and fusion ensure a healthy population of mitochondria and protect against potential damage or loss of the mitochondrial DNA which contains the genes necessary to carry out cellular respiration [7]. These processes play a significant role in the regulation of apoptosis [2,8-10] and have been implicated in normal developmental pathways as well as in the progression of neurodegenerative diseases such as Charcot-Marie Tooth or dominant optic atrophy [2,11-13].
To understand the mechanism of these mitochondrial processes, studies have been undertaken in yeast and mammalian cells - the results of which indicate that mitochondrial fission and fusion are mediated by a family of dynamin-related proteins (DRP's) [1,14-17]. DRP's function in membrane remodelling events such as budding, organelle fission and fusion, endocytosis, and cytokinesis [18-21]. These proteins do not work alone; in mitochondria, multi-component machines—fission and fusion complexes—are responsible for remodelling the inner and outer mitochondrial membranes to allow a tubule to divide or fuse [5,17]. Although the processes themselves are highly conserved, variations in the protein composition of the complexes do occur. For example, mammalian cells lack the adaptors Mdv1 and Ugo1 that function in yeast cells [1,3,17,22].
In an effort to understand the overall mechanism of these processes and the differences between organisms, a study of the lower eukaryote, Dictyostelium discoideum, was undertaken. It has been suggested that Dictyostelium cells do carry out mitochondrial fission [23,24], but no actual fission or fusion events have been identified. Thus, we developed an in vivo assay to determine if Dictyostelium cells carry out the processes of mitochondrial fission and fusion and to identify potential molecular players in these pathways. Dictyostelium expresses a number of proteins that are associated (based on function, homology, or localization) with the mitochondria, including two DRP and three dynamin-like proteins [21]. For this study, we chose the two DRP's, DymA and DymB, and two non-DRP's, MidA and CluA. MidA was chosen because it does not induce a change in mitochondrial morphology yet plays a significant role in mitochondrial function [25]. CluA was chosen because deletion of this protein induces a clustered mitochondrial morphology comparable to a fission defect in yeast cells [26,27]. Here, we present the first time-lapse images showing actual fission and fusion events in Dictyostelium cells. We quantified these events to establish that mitochondrial fission and fusion are balanced processes in these cells. In addition, we have begun to determine which proteins are essential for these processes. Our results reveal that MidA and the DRP's - DymA and DymB - are not required for mitochondrial fission or fusion. Interestingly, CluA appears to have some role in both processes as demonstrated by a decrease in both rates in the protein's absence. The results from this work will further our knowledge of the mechanisms of fission and fusion, ultimately contributing to a better understanding of both membrane dynamics and, more importantly, mitochondrial disease.
All Dictyostelium discoideum strains described here were obtained from Dicty Stock Center [28], AX4 (wild-type) was deposited by Bill Loomis, cluA- by Margaret Clarke, dymA- and dymB- by Dietmar Manstein, and midA- by Ricardo Escalante. The strains were cultured axenically in liquid HL5 medium supplemented with streptomycin (Fisher) (final concentration 300μg/ml) and ampicillin (Fisher) (final concentration 150μg/ml) at 22°C shaking at 125rpm [29].
AX4, midA-, and cluA- cells were diluted to 3 X 104 cells/ml in Lo-Flo liquid media (Formedium) until cells reached log phase (4–7days). dymA- and dymB- cells were diluted into HL-5 instead of Lo-Flo. Log phase cells (5ml) were washed by centrifuging at 500 x g for 4minutes and resuspended in 5ml of Lo-Flo (including dymA- and dymB-) plus 1μl of 1mM MitoTracker Red CMXRos (Invitrogen) [30]. Cells were incubated at room temperature with shaking for 3–5hours, washed twice with Lo-Flo, and resuspended in 5ml Lo-Flo media. Washed and stained cells (0.5ml) were placed in Lab TekII 4-well chambered coverglass (Nalge Nunc International) for imaging.
Quantified cells were imaged on a Zeiss laser scanning LSM Pascal confocal microscope using a pinhole of 144 (1.36 airy units), resulting in an optical slice of 1.1μm. A single plane was imaged every 677.38 milliseconds for 100seconds or until bleaching occurred. Fission and fusion events were identified in a minimum of 35 cells for each strain. Fission was identified when one organelle became two if, prior to this, no out-of-focus mitochondria had been visible. Fusion was identified when two mitochondria rotated around one another for a couple of frames (They never resolved into one during this time.) then became one. If two organelles came together, or split apart and were in their original state by the next frame, this was classified as a "drive by" and not quantified. Rates were calculated by averaging the number of events/min/cell for each strain and are presented as mean±standard error. Statistical analysis was performed using JMP 9.0.0 (SAS Institute, Inc) software. Wilcoxon rank-sum nonparametric t-tests were conducted for statistical comparisons of fission and fusion within and among strains; p-values less than 0.05 were considered statistically significant.
To be as stringent as possible with this assay, the data presented here has been repeated independently by a second individual who obtained statistically similar results (data not shown).
In an effort to confirm that the identified fission and fusion events are not from mitochondria entering and leaving the focal plane, small z-stacks were imaged. These included 3 planes of 1.1μm depth, images were flattened and quantified as above.
Recent work in the model organism, Dictyostelium discoideum, has suggested that the process of mitochondrial fission takes place; however, fixed-cell studies have conclusively demonstrated that Dictyostelium mitochondria are spherical organelles rather than the well studied tubular mitochondria found in most other organisms [20,21,23,24]. The simplicity of these spherical mitochondria suggests that the processes of fission and fusion are not necessary to maintain this organelle morphology. Thus, to determine if mitochondrial fission and fusion actually occurs in Dictyostelium cells, we developed a live cell fission and fusion assay. Upon first analysis of the mitochondria, it was apparent that there were too many organelles to clearly distinguish fission and fusion events in a whole cell (Figure (Figure1).1).
Using laser scanning confocal microscopy with a pinhole of 1.35 airy units, which produces a 1.1μm optical depth and Mitotracker Red (a well established mitochondrial marker) [30], a single plane of each cell was observed for fission or for fusion. The results demonstrated that Dictyostelium mitochondria do indeed undergo both of these dynamic processes (Figure (Figure2,2, Additional file 1). Upon quantification of these events, it was apparent that, like yeast, the average rates of fission and fusion were balanced (p-value=0.40), occurring at a rate of 1.00±0.12 (mean±standard error) fission events/min and 0.95±0.17 fusion events/min (Figure (Figure3).3). In order to ensure that identified fission and fusion events were not the result of organelles travelling into or out of the focal plane, small z-stacks (3 image planes of 1.1μm each) were imaged. Quantification of these flattened z-stacks indicated that fission and fusion still took place and were balanced; thus, the identified events in the single plane imaging were genuine and not the result of mitochondria travelling through the focal plane.
There are numerous proteins, based on function or homology, which may play a role in mitochondrial fission or fusion. We chose four of these proteins and used our assay to determine if they were involved in these processes. MidA is a putative methyltransferase that is targeted to the mitochondria and is required for mitochondrial complex I function [31]; it has been identified in Dictyostelium and humans [25] with a possible homolog in budding yeast [32]. Mitochondrial morphology of midA- cells is not altered from wild-type [25]; thus, we did not expect MidA to be involved in fission or fusion. Interestingly, when the processes were quantified in midA- cells, the fission rate (0.59±0.15 events/min) was significantly decreased as compared to the wild-type (p-value=0.0056). In contrast, the average rate of fusion (0.78±0.18 events/min) was not statistically different from wild-type (p-value=0.17). Despite the decrease of fission, the rates between the two processes are balanced (p-value=0.527) and there is no resulting morphological change so, most likely, MidA is not directly involved in mitochondrial fission (Figure (Figure3,3, Additional file 2).
Homologs of the Dictyostelium protein, CluA, have been identified in Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster,, Homo sapiens, and Saccharomyces cerevisiae. Mutations of this protein result in clustered mitochondria in all organisms with the exception of C. elegans (Figure (Figure4)4) [27,33,34]. In cluA-cells, fission and fusion rates remained balanced, with 0.64±0.15 fission events/min and 0.35±0.16 fusion events/min (p-value=0.079), but were significantly decreased as compared to wild-type cells (fission: p-value=0.0218; fusion: p-value=0.0001) (Figure (Figure3,3, Additional file 3). Interestingly, the fission and fusion events were only found among mitochondria not a part of a cluster. For example in Figure Figure44 and Additional file 3, fission and fusion can be seen in the top, less clustered cell but none were found in the bottom cell (Additional file 3). These results suggested that CluA is necessary to maintain the rates of mitochondrial dynamics at the level found in wild-type cells.
Here we analyzed the two DRP's, dynamin A (DymA) and dynamin B (DymB), for function in mitochondrial dynamics. Cells lacking DymA exhibit loose aggregates of both spherical and tubular mitochondria (Figure (Figure5)5) [20]. Analysis of dymA- cells showed that the mitochondria have an average fission rate of 0.98±0.12 events/min while fusion occurs at an average rate of 1.01±0.19 events/min (Figure (Figure3,3, Additional file 4). These rates were balanced (p-value=0.82) and were not significantly different from that observed in wild-type cells (fission: p-value=0.93; fusion: p-value=0.934). Thus, these results suggest that DymA is not essential for fission or fusion of the mitochondria in Dictyostelium cells.
DymB transiently localizes to the mitochondria - perhaps to complete post-translational processing- although deletions of DymB do not alter mitochondrial morphology [21]. In an effort to determine if DymB functions in mitochondrial dynamics, dymB- mitochondria were examined and the rates of fission and fusion were found to be balanced (p-value=0.83) and occur at a rate of 0.88±0.14 fission events/min and 0.92±0.14 fusion events/min (Additional file 5). These rates are not significantly different from AX4 (fission: p-value=0.38; fusion: p-value=0.99), therefore DymB is also not required for mitochondrial fission or fusion.
Here, we presented our findings on the processes of fission and fusion in Dictyostelium cells. Using our in vivo quantification assay, we demonstrated for the first time that, although Dictyostelium mitochondria are spherical in structure, their mitochondria do undergo fission and fusion. In further support of this result, Dictyostelium mitochondria morphology is very similar in appearance to peroxisomes. Peroxisomes have been shown to undergo the dynamic process of fission (though not fusion) [35]; thus, even spherical organelles require some dynamics. Based on the spherical structure, one might expect fission to occur more often than fusion to create the spheres rather than tubules that are found in other organisms. In Dictyostelium, the events are balanced, occurring approximately 1 event/min; interestingly, this is about twice as often as the rates published for yeast [5].
In an effort to begin identifying potential players in the fission and fusion processes, we examined four proteins that have some known association with the mitochondria. MidA appears to significantly alter mitochondrial function by disrupting the assembly of complex I in the electron transport chain [31]. Mutations in this protein result in numerous deleterious phenotypes ranging from small cell size and slow growth rate to a decrease in phagocytosis and macropinocytosis as well as a 70% reduction in ATP production [25].
Although fission and fusion are slightly decreased (fission more so than fusion), the rates are still balanced and mitochondrial morphology is not altered. This decrease in mitochondrial dynamics may be an indirect result of the inadequately functioning mitochondria. The work presented here suggests that MidA does not play a direct role in the processes of fission and fusion.
Transmission electron microscope images of cluA- cells indicate that the mitochondria are connected to one another, forming the characteristic cluster [24]. Our results demonstrate that there is a significant decrease in both fission and fusion processes of these mitochondria, resulting in a fission/fusion event approximately every two minutes. These collective results suggest that perhaps in the absence of CluA fission and/or fusion may be initiated but not completed, resulting in a connected cluster of organelles. The inability to complete the initiated events may be due to a loss of interaction with the cytoskeleton. CluA has homology to the protein-protein interaction region of the kinesin light chain - the tetratricopeptide repeats (TPR) domain [24] - suggesting that CluA could interact with kinesin and thus, the cytoskeleton. Studies performed in flies and plants support this conclusion [33,34]. However, there are many TPR proteins that do not interact with kinesin; an example is Fis1, a TPR protein which is required for mitochondrial fission in yeast and mammalian cells [36]. Ultimately, to tease out the specific role CluA plays in these processes, further studies will need to be pursued; for example, identifying interacting partners of CluA will help to determine if it connects to the cytoskeleton and/or to other potential fission and fusion proteins.
Finally, we looked at the classical proteins that mediate membrane remodelling events, the DRP's. Previous work suggests that both DymA and DymB have primary functions elsewhere in the Dictyostelium cell - endosomal pathway and vacuole/peroxisome dynamics respectively [20,21]. However, several proteins have been co-opted to work in multiple organelle remodeling events. In yeast for instance, both the peroxisome and the mitochondria use the DRP, Dnm1, to carry out fission [37]. Thus, we analyzed both DymA and DymB to see if one of these proteins may have been co-opted to work in multiple remodelling events. dymA- and dymB- mitochondria undergo fission and fusion about once every minute (equivalent to the rate found in wild-type cells) thus, DymA and DymB are not essential for these processes and have not been co-opted to mediate multiple organelle remodelling events. Although DRP's have been found to be responsible for mediating fission and fusion in all organisms studied to date, this is the first time it has been demonstrated that the classical DRP's are not required for mitochondrial dynamics. It is possible that the DRP-like proteins play this role in Dictyostelium or more likely the FtsZ homologs, FszA and FszB, as suggested by Gilson et al., 2003 [23].
In summary, we have shown for the first time that Dictyostelium mitochondria undergo fission and fusion like all organisms studied to date. The rates of these events are balanced, occurring at a rate of approximately 1 event/min. MidA is not essential for these processes nor are the classical DRP's, DymA and DymB, while CluA is necessary for both fission and fusion. This work contributes to our understanding of the overall mechanism of mitochondrial fission and fusion, but it is lacking the identification of the proteins specifically involved in these processes. Our goal is to use this assay to continue the search for these proteins, specifically asking if FszA or FszB might be responsible for mediating mitochondrial fission or fusion in place of the DRP's.
The data sets supporting the results of this article are included within the article and its additional files.
DRP, dynamin-related protein; DymA, dynamin A; DymB, dynamin B; TPR, tetratricopeptide repeats.
The authors declare that they have no competing interests.
BGS participated in image acquisition, quantification of mitochondrial dynamics and statistical analysis. GWB obtained and established all strains used in this study and participated in quantification of mitochondrial dynamics. KN conceived and designed the study and participated in image acquisition. All authors took part in drafting the manuscript and have read and approved the final manuscript.
MPEG (.mp4) Live cell movie of Dictyostelium mitochondria. Mitochondria in Dictyostelium cells are very dynamic, rapidly moving and undergoing fission and fusion. The circle indicates a fusion event occurring at 15–16seconds, the box indicates a fission event occurring at 8–10seconds. Single plane images were acquired every 677.38 milli-seconds with a 1.1μm optical depth by a 63X objective. The movie is presented at 2 frames per second.
MPEG (.mp4) Live cell movie of midA- mitochondria. Mitochondria in midA- cells are dynamic, undergoing fission and fusion, though slightly slower than wild-type cells. The circle indicates a fusion event occurring at 15–16seconds, the box indicates a fission event occurring at 14–18seconds. Single plane images were acquired every 677.38 milli-seconds with a 1.1μm optical depth, no averaging and a 63X objective. The movie is presented at 5 frames per second.
MPEG (.mp4) Live cell movie of cluA- mitochondria. Mitochondria in cluA- cells are clustered and have significantly decreased dynamics. The circle indicates a fusion event occurring at 40–42seconds, the box indicates a fission event occurring at 48–52seconds. Note: within the circle there are a series of fission and fusion events, but they are several frames apart. Single plane images were acquired every 677.38 milli-seconds with a 1.1μm optical depth, no averaging and a 63X objective. The movie is presented at 3 frames per second.
MPEG (.mp4) Live cell movie of dymA- mitochondria. Mitochondria in dymA- cells are slightly aggregated, may form tubules, or align into tubule like structures. Dynamics are similar to wild-type cells. The circle indicates a fusion event occurring at 22–24seconds; the box indicates a fission event occurring at 1–5seconds. Note: within the square, the movie begins a with fusion event distinctly separated by several frames before the fission event. Single plane images were acquired every 677.38 milli-seconds with a 1.1μm optical depth, no averaging and a 63X objective. The movie is presented at 3 frames per second.
MPEG (.mp4) Live cell movie of dymB- mitochondria. Mitochondria in dymB- cells are morphologically the same as wild-type cells with similar dynamics. The circle indicates a fusion event occurring at 1:09–1:12minutes; the box indicates a fission event occurring at 12–14seconds. Single plane images were acquired every 677.38 milli-seconds with a 1.1μm optical depth, no averaging and a 63X objective. The movie is presented at 5 frames per second.
Thank you to Brittany Sexson who established the Dictyostelium model system in our lab and to LaRhonda Apata and Jordan Bird who preliminarily established that fission and fusion are balanced. Thank you to Margaret Clarke, Vladimir Lupashin and Bhupinder Vohra for critiques of this manuscript. The project described was supported by grants from the National Center for Research Resources (5P20RR016460-11) and the National Institute of General Medical Sciences (8 P20 GM103429-11) from the National Institutes of Health. The Zeiss LSM Pascal confocal microscope used for image acquisition was funded by NSF Award Number 0215847.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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Ive tapered for running events but didnt realize how similar principles apply to swimming as well. Terry Laughlin from Total Immersion breaks it all down in Sharpening Your Swimming: Theories on Tapering. I think I need to get more serious with my swim before I can have a successful taper. What about you? Have you found an individualized swim taper that works?
My coach has an event each swim season called "**** Week", except it lasts from beginning/mid-December until mid/end of January. That's when practices get crazy and there's one day where we do 100 100's. Anyway. Once **** Week is over, right before Regionals and Metro, we have a taper week, granted that we've proved to him we deserve it during **** Week. During Taper Week, we do about 1000 yard practices, total. Last year, it really paid off. I got a PB at Regionals and received seventh in the region. (:
Absolutely. Periodization is as important in swimming as in any sport.
Personally, I try but it isn't overly planned. I'm a masters swimmer so don't train so much, plus make up my sessions with a number of coaches that don't talk to each other about what they are doing. But, as I approach key meets (to be honest, I don't do a lot of small meets) I will certainly reduce my training load and do more quality work. I'll also come off the weights.
Rob Robson
Co-founder, iStadia.com sports community | fitness & sports jobs | olympic news
ACTIVE is the leader in online event registrations from 5k running races and marathons to softball leagues and local events. ACTIVE also makes it easy to learn and prepare for all the things you love to do with expert resources, training plans and fitness calculators.

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The Miami Project to Cure Paralysis received permission from the FDA to begin a Phase I clinical trial to evaluate the safety of transplanting human Schwann cells to treat patients with recent spinal cord injuries (SCIs).
Found mainly in the peripheral nervous system, Schwann cells are essential to sending appropriate electrical signals through the nervous system, and Miami Project scientists and supporters believe they are key to finding cures for paralysis. In what will reportedly be the only FDA-approved cell therapy-based clinical trial for sub-acute spinal cord injury in the U.S., investigators plan to transplant a patient's own Schwann cells at the injury site in the hope of ascertaining safety that will allow further trials to proceed.
The clinical trial will enroll eight participants with an acute thoracic SCI. Newly injured patients brought to the trauma center would have to meet the stringent criteria and agree to participate in further screening within five days of their injury. At that point, the participant will undergo a biopsy of a sensory nerve in one leg to obtain his or her own Schwann cells. The Schwann cells will then be grown in a culturing facility for three to five weeks to generate the number of cells necessary for transplantation, and to undergo the strict purification process. By the time the Schwann cells are surgically transplanted into the injury site, participants will be 26–40 days post-injury.
All procedures will be conducted in Miami at University of Miami Hospital, Jackson Memorial Hospital, and The Miami Project to Cure Paralysis. Each participant will be followed intensely for one year after receiving the transplantation surgery, and their neurologic status, medical status, pain symptoms, and muscle spasticity will be evaluated. All participants will continue to be monitored for years under a separate clinical protocol. This Phase I trial is the foundation upon which The Miami Project will develop future trials targeting different types of injuries, times post-injury, and therapeutic combinations.
The Miami Project is a comprehensive spinal cord injury research center and a designated Center of Excellence at the University of Miami Miller School of Medicine. The project's Christine E. Lynn Clinical Trials Initiative takes discoveries found to be successful in laboratory studies and fast-tracks them to human studies.
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MDMA (commonly known as the empathogen "ecstasy") is classified as a Schedule 1 drug, which is reserved for compounds with no accepted medical use and a high abuse potential. Two researchers from Stanford, however, call for a rigorous scientific exploration of MDMA's effects to identify precisely how the drug works, the data from which could be used to develop therapeutic compounds.Do you agree that ecstasy should be studied for its potential therapeutic benefits?
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DrySpec Bottle Mount KitCarry extra fuel, oil, water or other vital fluids on the outside of your sidecase with the DrySpec Bottle Mount Kit. Learn More$19.99
DrySpec Siderack Accessory Mounting Bracket KitWant to mount your Tool Tube or Bottle Mount to your SW-MOTECH siderack? These brackets which can mount in an infinite number of positions are the answer. Learn More$24.99
Control Mount (Harley-Davidson and Metric)Fits wide range of metric and all Harley-Davidson motorcycles.Control Mount: attaches to handlebar control clamps(clutch or brake side). Replaces the two factory pinch bolts with new stainless bolts and spacers on handlebar controls Learn More$79.95 $34.99
Stash Box Lockable License Plate Storage Box by Twisted ThrottleThe STASH BOX is a weather resistant storage box that mounts between the motorcycle and the license plate. It is ideally suited to storing the vehicle registration and other small objects while remaining unobtrusive. The Stash Box has features that provide benefits over other motorcycle storage devices i.e., low cost, concealed mounting location, vehicle styling is unaffected, and easy accessibility. Learn More$21.99
Oxford Maximiser 360T - multi-purpose 12-volt motorcycle, car, truck & marine battery charger (Discontinued)
Note: The Oxford Maximiser 360T - multi-purpose 12-volt motorcycle, car, truck & marine battery charger has been discontinued and is no longer available in the USA. Please use the toggle button on the upper right of your screen to switch to our Canadian website
Oxford's smart 3600mA charger with multiple charging rates which analyzes battery condition, will check polarity, and "float", or maintain a charge for battery longevity. Suitable for all 12 volt motorcycle, car, truck and leisure batteries.
Designed as a replacement for the popular 360T charger, this professional switch mode battery charger is designed to automatically maintain, optimize and lengthen the life of your bikes battery. New voltage sensors detect high current drain faster and will switch automatically to the most appropriate charge rate and will charge from as low as 6 volts to increase the chance of some batteries.
Please Note: Charger not suitable for Lithium type batteriesLearn More$74.99 $41.99
Oxford Oximiser 600 - the essential 12-volt motorcycle battery chargerQuickly charges battery at 600mA/hour until full and maintains a gentle trickle charge to keep battery optimized. Quick-connect leads included for easy coupling to the Oximiser between rides. Wall Bracket and extra long cables make for ease-of-use in your garage. Learn More$39.95
R&G Front Axle Sliders / Protectors for Triumph Bonneville, Scrambler '06-'09 & Thruxton '06-'16R&G Front Axle Sliders / Protectors. These fork protectors were designed to help eliminate damage to the front axle during a drop, a skidding stop or a "Low Side". Learn More$59.99
R&G Waterproof Motorcycle Cover for Supersport and Naked Bikes - BlackFits most sport and naked bikes without hard sidecases or topcases. Waterproof outdoor protection with anti-theft features and paint protection built in. Learn More$79.99
SW-MOTECH Waterproof Case for iPhone 4s, iPhone 4 & iPhone 3Keep your iPhone protected, dry, and charged with the Biologic iPhone case. The waterproof Biologic case snaps quickly on and off of the motorcycle and has a touch-sensitive screen that won't slow you down. Includes 2 silicone liners that provide a secure fit and protection to your iPhone 3G, 3Gs, 4, and 4s. Learn More$70.95 $31.99
R&G Waterproof Motorcycle Cover for Adventure BikesDesigned to fit larger adventure and dual-sport bikes with or without sidecases and topcases. Waterproof outdoor protection with anti-theft features and paint protection built in. Learn More$92.99
Techmount Universal 7/8" (22mm) up to 1 1/4" (32mm) Handlebar Mount, black, silver or chromeThis is the Techmount 3rd generation mounting system. They were able to combine all of the current production handlebar sizes from 7/8 (22mm) up to 1 ¼ (32mm) into one mount system. This allows it to fit virtually every motorcycle with tubular handlebars. Requires only ¾ of handlebar space and can be mounted at any angle. Available in Black, Silver oe Chrome. Having no need for a shaft, makes this mount system very strong and very compact. Learn More$79.95 $34.95
SW-MOTECH Bags-Connection Luggage Security Cable with Combination Lock
The SW-MOTECH Bags-Connection Cable Lock Lock is ideal for for securing the contents of any tankbag or rearbag. The ultra-compact design makes these locks easy to carry. Simply slide through any compatible zipper pulls and the frame of the bike and engage the lock.Learn More$20.95
SW-MOTECH HAWK Light Clamp Set For 22mm, 26mm, 27mm or 28mm OD (Outer Diameter) Tube Crash Bars
The SW-MOTECH light mount kit is designed to mount HAWK fog / off-road lights to crash bars with 22, 26, 27 or 28 mm OD (outer diameter).Learn More$41.95
SW-MOTECH Universal GPS Mount Kit | Medium Navi bagThe SW-MOTECH Universal GPS Kit allows for a variety of installation options without additional accessories, and includes a medium splash-proof NAVi bag to protect your GPS from the elements. Learn More$83.95
Denali Fender or Bolt Mount Kit For Denali DM Auxiliary LED Headlights
This Denali Fender or Bolt Mount Kit allows you to mount the Denali DM lights onto your front fender or other exposed mounting bolts.Learn More$24.99
Denali Universal Articulating Mounts to fit Denali DM, D1, D2, D4, DR1 & DX Auxiliary LED HeadlightsConstructed from black anodized billet aluminum for strength and durability, the Denali LED Lighting Universal Offset Mount Kit provides 3 axis adjustability with a 1.75" (45mm) offset that mounts to any M5, M6 or M8 bolt point on your motorcycle. Learn More$59.99


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January 28, 2013
It's the most viewed non-commercial site with over a million hits a week. People send in their secrets on a creative postcard and have them posted on the website called PostSecret. What has made the website even more popular are the videos that are posted, videos that are created in Pittsburgh. We take you on the road to meet the creator of PostSecret as well as some local people who have made PostSecret so popular.
Coming Up Next on Experience
Monday, August 1, 2016
Leo Beachy: A Legacy Nearly Lost


text:
Not at his age. For one its more like $1.5 of his $2.7M
The magic of investment's past is not the same. You and I may be able to make piles of money starting with that amount, but not your average intelligence football player. And by his maneuvers, he has shown less than average intelligence.
He's probably already spent half a million of it on his agent, financial adviser and the local nude bar girl.
Something seems different with Mike Wallace
by Mark Kaboly
Wide receiver Mike Wallace, or the self-proclaimed "Fast Money" member of the "Young Money Crew" likely won't get his "Big" money from the Steelers. Wallace and the Steelers have until approximately 3 p.m. Saturday to sign a long-term deal, or Wallace will play the final year of his contract without any assurances for the future. Maybe that's why Wallace has seemed different in the locker room ever since his return last week. Usually, Wallace is engaging with his teammates, as well as the media. This year, he doesn't appear to be interested in flashing that infectious smile and outgoing personality -- sort of like he has other things on his mind. Now, it's early, but could this be a precursor of things to come especially if Wallace and the Steelers don't get a deal done within the next few days? If a deal doesn't get done, it is going to be interesting to see if Wallace will be able to let that not affect him.
Hard as it would be to believe, maybe Mike actually thought he was going to get Fitzgerald-type money.
1 CB Vernon Hargreaves Florida 5-10 204
2 DT Austin Johnson Penn State 6-4 314
3 SS Jeremy Cash Duke 6-0 212
4 LB Jaylon Smith Notre Dame 6-2 223
6 WR Moritz Boehringer Germany 6-4 227
7 OT Brandon Shell South Carolina 6-5 324
7 RB Tre Madden USC 6-0 223
DE David Onyemata, LB Ian Seau, FS Jordan Lomax, CB DeAndre Elliott,
QB Jeff Driskel, TE David Morgan, OG Spencer Drango, OC Mike Matthews
2015 MNF Executive Champion!


text:
Vicky Turk, 79
Published: Wednesday, January 5, 2011 at 10:23 a.m.
Last Modified: Wednesday, January 5, 2011 at 10:23 a.m.
HENDERSONVILLE — Vicky Turk, 79, of Hendersonville, died Monday, Jan. 3, 2011, at Hendersonville Health & Rehabilitation.
No services are planned.
Thos. Shepherd & Son Funeral Directors is in charge of arrangements.
Reader comments posted to this article may be published in our print edition. All rights reserved. This copyrighted material may not be re-published without permission. Links are encouraged.


text:
Looking to class up the grill? Fire it up with flavor! Marion, Anthony, Rosanna, John and Elaina, of the New York restaurant Fresco by Scotto, share their recipes for delectable entrées and desserts from Italy — including herbed salmon and butterscotch banana pouches:
- 4 (2-pound) semi-boned whole chickens split in half, thigh and breast bone removed
- Nonstick vegetable oil spray
- 8 bricks, each wrapped in foil
- 1/4 cup lemon juice
- 4 garlic cloves, crushed
- 11/2 cups extra virgin olive oil
- 1 teaspoon fresh oregano
- 1 teaspoon chopped rosemary
- Salt and pepper to taste
Baking Directions:
1. In a large bowl, mix together lemon juice, garlic, extra virgin olive oil, oregano, rosemary. Place the chicken breasts in a large baking pan and pour the mixture on top; marinate for 12 hours in the refrigerator. 2. Spray grill rack with vegetable oil spray and prepare barbecue medium high heat. 3. Re-season chicken with salt and pepper, place on grill skin side down and top each piece with a brick. Grill 10 minutes per side under brick until skin is golden brown and crispy. Serve immediately.
- 1 (2-pound) salmon filet, skin removed
- 1 cup bread crumbs
- 6 cloves garlic, chopped fine
- 3 tablespoons basil, chopped fine
- 3 tablespoons parsley, chopped fine
- 3 tablespoons shallots, chopped fine
- 3 tablespoons chives, chopped fine
- 1/4 pound butter
- 1/2 cup extra virgin olive oil
- Salt and pepper to taste
- Aluminum foil
Baking Directions:
1. Place salmon filet on cutting board, carefully butterfly lengthwise, being sure not to cut completely through the flesh at the end. You want to be able to open it up into a thin double-width fillet. 2. In a large bowl, combine bread crumbs, garlic, basil, parsley, shallots, chives and extra virgin olive oil and mix thoroughly. 3. Cut a piece of aluminum foil the length of the salmon filet, large enough to go around the salmon when it's rolled. Lay salmon fillet flat on foil, season with salt and pepper, sprinkle bread crumb mixture evenly over the surface and place small pats of butter about 1-inch apart. 4. Roll salmon fillet with the foil, as you would a jelly roll; twist ends of the foil together. Refrigerate for 2 hours. 5. Cut rollatina into 2-inch thick slices leaving the foil intact. 6. Grill salmon slices 5 minutes on each side. When fully cooked, remove foil and serve immediately.
- 1 banana, quartered
- 3 tablespoons light brown sugar
- 2 tablespoons unsalted butter
- Pinch cinnamon
Baking Directions:
1. Place 12-inch square aluminum foil on work top. In the center, place the butter and top with the brown sugar, banana quarters, and cinnamon. Fold up securely and place on grill for 5 minutes. Open carefully and serve with almond pound cake and/or vanilla ice cream.
- 1 1/4 cups cake flour
- 3/4 teaspoon baking powder
- 1/2 teaspoon salt
- 9 ounces chilled almond paste
- 1 1/2 cups chilled granulated sugar
- 1 1/4 cups chilled unsalted butter, cut into pieces
- 6 large chilled eggs
Baking Directions:
1. Preheat the oven to 325°F. Butter and flour a 9 x 5-inch loaf pan or an 8-inch round cake pan. 2. In a small bowl, whisk together the flour, baking powder, and salt. 3. In a food processor fitted with a metal blade, combine the almond paste and sugar and pulse 3 to 4 times, or until the mixture forms fine crumbs. 4. Add the butter and pulse 8 to 10 times, until well mixed. Transfer to the bowl of an electric mixer. 5. With the mixer on low speed, add the eggs, one at a time, beating after each addition to incorporate the egg before adding the next one. Mix until the batter is light and fluffy. 6. Using a rubber spatula, fold in the flour mixture. Scrape into the prepared pan and bake on the center rack of the oven for about 1 hour 15 minutes, or until a toothpick inserted in the center comes out clean, the cake begins to pull away from the sides of the pan, and the top is a deep golden brown. Cool completely on a wire rack.


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Fraternity Vacation
Release Date: January 1st, 1985
R|1 hr. 33 min.
Plot Summary
An Iowan (Stephen Geoffreys) and his college buddies head for Palm Springs, Calif., to find the perfect blonde.
Cast: Stephen Geoffreys, Sheree J. Wilson, Cameron Dye, Leigh McCloskey, Tim Robbins, John Vernon, Britt Ekland, Nita Talbot
Director: James Frawley
Genres: Comedy


text:
Organize your crafts with these easy oilcloth envelopes, complete with tags.
If, like me, you tend to accumulate projects-in-progress, you might want to stitch up a bunch of these easy oilcloth holders. They keep all the needed supplies together, neat and tidy, and they're structured so little things can't easily fall out. They store easily on a shelf or in a bin, and when you're ready to craft, you can read the handy hanging tag to find the project you want.
Materials Note: If you want to make a lot of Project Keepers on the cheap, watch your local thrift stores for vinyl tablecloths.
Step 1: Decide on the size you want your finished Project Keeper to be, and then follow the above diagram to cut a piece of oilcloth or vinyl. Size your Keeper a little generously, as the side seams will take away about 1" in width.
Step 2: You should end up with a long rectangle of oilcloth or vinyl. If you like, trim both short edges with pinking shears or decorative scissors.
Step 3: Fold the rectangle almost in half, leaving about 3" at the top, as shown. Crease the fold well with your fingers. This step helps you identify the front panel of the Keeper.
Step 4: If you like, you can add a clear vinyl pocket to the outside of your Keeper. I like these pockets because I'm always trying to keep my craft tools with my WIPs (works in progress), but then I usually end up wanting those tools later for another project. I store scissors, hooks, needles, etc. in the outside pocket of the Keeper so I can find them easily. If you want to add a pocket, unfold the rectangle so it's a single layer again. Cut a scrap of clear vinyl to the pocket size you want. Tape it to the front panel of the Keeper with masking tape.
Step 5: Sew along 3 sides of the pocket, leaving the top edge open. You can sew right over the tape and pull it off afterward.
Step 6: Refold the piece as in Step 3. Place a couple tabs of masking tape around each side to hold this fold in place.
Step 7: Fold that extra 3" over the front of the Keeper. Make sure this flap overlaps the front panel no more than 3/4". Tape the flap down as well.
Step 8: Sew along both sides, through all layers, using a 1/2" seam allowance. Again, you can sew right over the tape and peel it away afterward.
Step 9: If you like, you can trim the seam allowance with decorative scissors or pinking shears.
Step 10: Next, make the hang tag. Cut out the front and back pieces from black vinyl, following the shapes shown here. Cut a clear piece to fit between them as a window. Stack the three pieces together, with wrong sides together and the clear vinyl in the center. Tape them, and sew along 3 edges and the top as shown above. Leave one side open so you can slip in a handwritten label later.
Step 11: Sew a button to the front panel of the Keeper, about 2" below the top edge. Make sure it's centered.
Step 12: Thread a chenille needle with about 36" of embroidery floss or twine. Pass the needle up through the top of the tag from back to front. Then, pass it back down to form a small loop, as shown here.
Step 13: Pull the floss through until the ends are even, and then pass the ends up through the loop. Pull the floss to make a slip knot.
Step 14: Thread the 2 ends of the floss together on the chenille needle. Bring the needle up through the flap, passing it from inside to outside. Center this stitch above the button.
Step 15: Pull the floss until the tag is hanging about 6"-8" below the stitch, as shown.
Step 16: Then, pass the needle back down through the flap, forming a small stitch.
Step 17: Tie the ends of the floss in a secure double knot at the inside of the flap. Trim away the end of the floss that isn't attached to the tag.
Step 18: To operate this closure, just wind the floss around the button a few times.


text:
Comic-Con Trailers: The Best of the Best, Ranked
Film, TV
Showing results 1-6 of 6
Writer Stuart Moore spoke with CBR News about opening worlds of possibilities in 'Shadrach Stone," a multiversal graphic novel from Penny-Farthing Press, as well as his thoughts on the existence of real alternate worlds.
The writer behind the new graphic novel "Anne Steelyard and the Garden of Emptiness: An Honorary Man" from Penny-Farthing Press talks about her first comics project, historical writing and the inspiration Gertrude Bell.
Archives: All | Comic Books | TV/Film | Video Games | Web Comics


text: Rewatched Dark Knight Rises on blueray-was reminded of how weird the scene is when Gordon is sentenced and Bane orders Miranda to be taken to him. Next she's back with Fox when Bruce is fake captured and then next we know she's back with Bane. It's weird bit of scene following or editing.


text:
Hot tags Galaxies Blackholes Planets and Asteroids Space Exploration Telescopes
Science subject and location tags
Articles, documents and multimedia from ABC Science
RSS | MapAstronomy (Space)
Milky Way Galaxy has up to three times more star forming material than thought
Wednesday, 21 October 2015
Star power The Milky Way Galaxy has enough star forming molecular clouds to keep making stars at the present rate for another six billion years.
Jupiter's changing Great Red Spot
Monday, 19 October 2015
StarStuff image The Hubble Space Telescope has captured a new portrait of the solar system's largest planet Jupiter showing more changes in its famous Great Red Spot.
Best evidence yet that Mars was warm and wet
Wednesday, 14 October 2015
StarStuff Podcast Rounded pebbles indicate ancient Mars had running streams and long periods of warm wet climate. Also; Earth's inner core is far younger than previously thought, and stunning new images of blue Earth-like skies on Pluto.
Veil Nebula's tortured tendrils mark a violent past
Monday, 12 October 2015
StarStuff image of the week Beautiful filaments of gas mark one of the most violent events in the universe.
New Horizons reveals blue skies and red water ice on Pluto
Friday, 9 October 2015
Stunning Pluto Pluto has blue skies and patches of red frozen water, according to the latest data from NASA's unmanned New Horizons probe, which made a historic flyby of the dwarf planet in July.
Asteroid and volcanoes combined killed the dinosaurs
Wednesday, 7 October 2015
StarStuff Podcast The asteroid that slammed into Earth 66 million years ago accelerated volcanic eruptions in India creating a double-whammy disaster that wiped out the dinosaurs. Also; new insights into the dwarf planet Ceres, and neutrino discovery awarded the Nobel Prize in Physics.
Detailed maps reveal Ceres' mysterious surface
Friday, 2 October 2015
Mysterious Ceres New maps of Ceres provide insights into the dwarf planet's mysterious bright spots and pyramid shaped mountains.
Signs of water discovered on Mars
Wednesday, 30 September 2015
StarStuff Podcast Astronomers confirm dark streaks running down cliffs on the Red Planet contain evidence of flowing liquid water. Also; Rosetta's comet 67P consists of two separate comets that merged early in the history of the solar system, and new computer simulations reveal how the brightest galaxies in the universe were formed.
Finding water on Mars is huge for our search for life beyond Earth
Tuesday, 29 September 2015
Opinion This isn't some long-gone lake bed from a billion years ago, there is water flowing on Mars right now. This discovery is a huge step forward in our search for life, writes Alan Duffy.
Evidence of liquid water found on Mars
Tuesday, 29 September 2015
Wet planet Scientists analysing NASA data say they have found evidence of flowing water on the surface of Mars, but caution there is a "long way to go" before they can say if the Red Planet could support life.
A cosmic rose with many names
Monday, 28 September 2015
Starstuff space image Blossoming like a giant cosmic rose, Messier 17 is a stellar factory where new stars are born.
Pluto: New Horizons images reveal stunning views of 'Arctic-like' landscape
Friday, 18 September 2015
Stunning world Stunning new images of Pluto backlit by the sun reveal a landscape of majestic mountains, foggy plains and hazes.
Voyager's recorded messages for aliens
Thursday, 17 September 2015
Sounds of science A recording of Earthly sounds and images is hurtling through interstellar space at 60,000 kilometres per hour on the off chance it runs into aliens.
Global ocean discovered on Saturn's moon Enceladus
Wednesday, 16 September 2015
StarStuff Podcast A global ocean discovered under the frozen surface of Enceladus a new target in the hunt for life beyond Earth, Also; Ceres' bright spots still a mystery, and world's longest continental hotspot chain discovered under Australia.
Global ocean extends across entire core of Saturn moon
Wednesday, 16 September 2015
Big ocean NASA researchers have discovered a global ocean underneath the icy surface of Saturn's moon Enceladus.


text:
|Message view
|« Date » · « Thread »
|« Date » · « Thread »
|"Tako Schotanus" <quinte...@gmail.com>
|Re: David's Model: A guide for blissful content modeling
|Tue, 07 Aug 2007 22:28:19 GMT
On 8/7/07, David Nuescheler <email@example.com> wrote: > > Hi, > > thanks for sharing the content model. > > from my perspective i think the separate workspaces are not justified: > > http://wiki.apache.org/jackrabbit/DavidsModel#head-ca639e0ee110b80e8277a50f9b9de092b5d86427 > > ... and personally i would not separate "by type" in the hierarchy. > (or at least not at a root level). i would propose a hierarchy in single > workspace that looks roughly like this... > > /artists/david/myproject1/folders > > /artists/david/myproject1/folders/a-song.txt > /artists/david/myproject1/folders/a-song.mp3 > > i would probably use the jcr:mimeType property or an extension > of the nt:resource mimetype to tell your resources apart. Except maybe if you plan on adding type-specific properties (not something that appears in his model, mind you), like extracts or stripped (text only) versions for the text nodes, thumbnails for the image and video nodes and type-specific meta info. I'm not saying that is the way to go, but it might be a reason to choose type-specific nodes, so that you know what kind of extra data to expect. Of course you could do the same by adding special mixins for each node depending on the mime-type. The only time when you really might need special nodes for each content category is when you want to make sure that the content is of a specific type. Dunno, let's say you want to store company letter templates for a text processor and each has to have a text node and an image with the company logo. That's a requirement that's easy to express in the model if you have those content categories defined. If you never going to have those kind of requirements then David's suggestion is much more flexible. Cheers, -Tako regards, > david > > > On 8/7/07, paksegu <firstname.lastname@example.com> wrote: > > Hi, > > After reviewing "David's Model: A guide for blissful content modeling" > what comments and advice can you offer on this approach? > > > > > https://www.dev.java.net/files/documents/4363/44912/SerenadeRepository.pdf > > > > Thanks > > > > > > Ransford Segu-Baffoe > > > > email@example.com > > > > https://serenade.dev.java.net/ > > http://www.noqturnalmediasystems.com/ > > > > --------------------------------- > > Pinpoint customers who are looking for what you sell. >
|View raw message


text:
Turkish police have arrested 10 people on charges of suspected links to Al-Qaeda, local media reported on Tuesday.
The suspects are claimed to have provided weaponry and recruits for elements of the Syrian opposition, charges they vehemently deny, the Hurriyet newspaper said on its website.
"We've been arrested just because we recite the Koran and we are Muslims aiding Syrians," it quoted one of the suspects as saying.
Police conducted the raid to arrest the suspects in the central Anatolian province of Konya as part of an operation against the radical organisation.
Turkish security forces have regularly targeted suspected Al-Qaeda supporters since twin suicide bombings hit Istanbul in November 2003.
A Turkish cell of Al-Qaeda was held responsible for those attacks, in which explosive-laden trucks first targeted two synagogues, and then the British consulate and a British bank, killing a total of 63 people, including the British consul.
Turkish media reported last week that police have uncovered and foiled an alleged plot by Al-Qaeda to bomb the US embassy in Ankara, as well as a synagogue and other targets in Istanbul.
The embassy in Ankara was the target of a suicide bombing on February 1, which killed a Turkish security guard. That attack was claimed by a radical Marxist and anti-US armed group, The Revolutionary People's Liberation Front (DHKP-C), blacklisted by the US and the European Union as a terrorist organisation.


text:
Was Daniel Patterson "Forced to Resign"?
State Representative Daniel Patterson resigned from the House yesterday, before a vote on his expulsion could take place.
The House Ethics Committee unanimously voted earlier yesterday to boot Patterson, which was apparently enough of an indication for Patterson to just take off early.
"I have been forced to resign due to the fact that the House has become a very hostile work environment for me," Patterson said in his resignation letter. "Due to this I am no longer able to serve my constituents in the way they deserve."
Just an hour before House Speaker Andy Tobin accepted Patterson's resignation, Patterson took to Twitter -- where he's routinely posted responses during the ethics investigation process -- saying the "Fix was in."
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Patterson's decried pretty much every aspect of the investigation since day one.
Even on the day the ethics complaint was filed against Patterson -- February 27 -- he denied the allegations to New Times. He continued to issue denials throughout the entire process.
Patterson's continued to insist that he deserves a full hearing with witnesses testimony and the whole bit, calling for "proper due process," but he obviously didn't get what he wanted.
"The unfair Ethics Committee violated House Rules, my constitutional rights to due process, and would not even allow me a real hearing to defend myself and question accusers," Patterson wrote in his resignation letter. "The rights of Arizona voters and constituents in Tucson LD29 have been badly disrespected by the Ethics Committee. The will and decision of voters has been violated again by the House."
This morning's question: Was Patterson "forced to resign"?
Cast your vote below:
Also read:
Daniel Patterson Calls it Quits, Resigns From House
Daniel Patterson Rejects Accusations, Fellow Legislators Say It's Like Trayvon Martin Case
Daniel Patterson's Political Career Lives Another Day; His Response to Report Due Next Week
Ethics Investigators: Daniel Patterson Is Rude, Has Tried to Trade Favors for Votes, Violated Court Orders, and Has Got to Go
Daniel Patterson Responds to Ethics Complaint, Says No One Knows Nothin'
Daniel Patterson's Ex-Girlfriend Now Says Legislator "Should Be Found Innocent"
House Ethics Complaint Filed Against State Rep. Daniel Patterson After Domestic-Violence Claim
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text:
Director found for The ExpendaBelles
The female-centric take on The Expendables has landed a director. Plus: first plot details.
With a bit of luck, the title of the female-slanted Expendables equivalent will change by the time the film comes to release. The idea of an action movie headlined by woman doing lots of damage is welcome, but calling it The ExpendaBelles? Yikes.
Anyway, there's been some progress on the film. It's been revealed that Robert Luketic has been hired to direct the movie. He's had a varied career to date. Our favourite of his films are 21 and Legally Blonde. His latest, Paranoia, is heading straight to DVD in the UK next month.
Oh, there's an outline plot too. It reads...
"When America's Navy SEALs are wiped out trying to penetrate the island lair of a deadly despot who has captured one of the world's top nuclear scientists, it becomes clear that there is no such thing as the right man for the job and that this is a mission so impossible that only women can handle it. The only way in: some of the world's deadliest female operatives must pose as high-class call-girls shipped in by private plane to satisfy a dictator–and instead save the scientist and the day"
Yep: the only way you can get an all-female action movie is to make them call girls. Bet you a fiver that the action heroes of The Expendables 4 won't be rent boys.
There's no timescale that we know of, but with Expendables movies arriving every two years - with another this summer - we wouldn't rule out the summer of 2015. More as we hear it.
Follow our Twitter feed for faster news and bad jokes right here. And be our Facebook chum here.


text:
APICS News
APICS Southeast District News
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APICS Triangle First Student Case Competition a Success!
The APICS Triangle Chapter held its first ever Student Case Competition last week in Raleigh, North Carolina. Students from NC State and Duke University participated in groups of three where they were asked to review a supply chain related case study and submit their findings and recommendations to a panel of judges appointed by the APICS Chapter Board. The top 5 groups presented their findings and recommendations at last Tuesday's Professional Development Dinner Meeting on the evening of April 5th.
The Triangle Chapter would like to take the opportunity to thank all the students who participated in the competition and would like to recognize the following finalists for their detailed analysis and recommendations:
From NC State University:
Sourabh Deshmukhe, Tanul Kala, Tanmay Lohani, Saurich Yelchur Sanath, Sanath Pavate, Disha Hitesh Kanara, Atchyuta Bharadwaj Manda, Indraja Vemana and Krishna Balabadhrapatruni
From Duke University:
Sai Aditya Kolluru, Chaithra Cheruku, Anirudh Yedulla, Ai Lin Loh, James Huang and Masayuki Kobayashi,
Special recognition goes out to the winners Ai Lin Loh, James Huang and Masayuki Kobayashi from Duke University's Masters of Engineering Management Program.
We would also like to thank the panel of judges (Tim Rhoads, Prasanna Deshpande and Ben Poston) for their participation and for lending their supply chain expertise to judge this event
Documents from our Tampa Meeting - February 2016
District Meeting 2016 February Tampa
Bell - APICS For Business & BDM UpdateDoug Bell - Feb 2016 Tampa
Thatcher - Chronic DisruptionThatcher February 2016 Tampa
Williams - APICS Programs 020616Williams - February 2016 Tampa
Southeast District Business Development Program 2.0
APICS is please to announce the new channel partner pro...
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Pictures From Our Louisville KY Meeting June 2016
APICS Chapter News
Events, Meetings, and Webinars
July Webinar
On Line Webinar
July 27, 2016
On Line Webinar
12:00 PM to 1:00 PM
"Power Play: An Overview of the Strengthening U.S. Manufacturing Industry"
July 27, 2016
12:00 PM to 1:00 PM
August Webinar
On Line Webinar
August 25, 2016
On Line Webinar
12:00 PM to 1:00 PM
"Lead Differently: To Engage the New Workforce, Dive in to Different"
August 25, 2016
12:00 PM to 1:00 PM
APICS Southeast Webinar Series
Links to past webinars
- Setting Up Successful Pilots"
- Recorded March 24, 2016
- PDF of Presentation
- The YouTube link: https://youtu.be/QsXOc8D4tms
- "What's In Your Weather Warning"
- Recorded February 23, 2016
- Featuring Gene Norman
- PDF of presentation
- YouTube link: https://youtu.be/7SG1gLy_sZ4
- "Priming Your Supply Chain for the Amazon Effect"
- Recorded January 28, 2016
- Featuring Lisa Anderson, MBA, CSCP
- PDF of presentation
- YouTube link: https://youtu.be/v5a49lq_eJo?t=1s
- "How to Grow your APICS Chapter's Education Sales"
- Recorded 8-December-2015
- Featuring Gail Rutkousky
- YouTube link: https://youtu.be/sOcxM4ip8z4
- PDF of Presentation: http://tinyurl.com/out8zg8
- "The Seven Deadly Sins of Sales Forecasting"
- Recorded 28-October-2015
- Featuring Fred Tolbert
- YouTube link: https://youtu.be/2oD2OPqo1aw


text:
Gulere among Pilgrims from Mecca and medina at bole International Airport. He was unable to return home for two days after the exchange because the flights were all fully booked during the season.
Gulere with a friend referred to as Kungu (not real name) who provided the first fruit of hospitality
Waiting for the ferry
On the ferry
vehicles on the ferry
Freetown streets
cartoon at Pen
Executive members of Sierra PEN
Sierra School PEN Club members
All as one
Sherrif gesturing
Gulere departs
Forging ahead
My Wish
Thank you
In memorium
Smith chairs the Literary evening
Prof. Amadou talks
We need power..
School clubs task force skit
"For you and your wife from me and my wife" Moses Kainwo
Resource room
Recitals by Moses
Anti corruption center
Meeting point of slave returnees
No life jackets
In the press
Write and write and write
Let's dine and write
Presidential speech
the leir
We can make it
graduates challenge
writers abroad


text:
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