text:
History Channel's Ice Road Truckers wrapped up its ninth season Sunday as the wilderness roads of northern Canada thawed, lakes melted, and the truckers who crossed them went home, writes Overdrive sister site's Truckers News Editor David Hollis in his weekly recap.Read More
There's only one way to describe Sunday night's episode of History Channel's Ice Road Truckers, and that's "mud-ugly," writes Overdrive sister site's Truckers News Editor David Hollis in his weekly recap.Read More
The arrival of spring and the melting of ice and snow was not what the drivers on History Channel's Ice Road Truckers wanted, writes Overdrive sister site's Truckers News Editor David Hollis in his weekly recap.Read More
Sunday night's episode (Season 9, Episode 6) of Ice Road Truckers was one of the busiest, maybe best, episodes of Season 9 so far, according to David Hollis of Truckers News.Read More
Best Quote of Episode 2 goes to Darrell: "The more loads I deliver on time, the more loads I will have to deliver." ...Read More
The new, ninth season of the long-running series follows a cast of new and familiar faces on far-Manitoba ice roads, debuting this Sunday on the History Channel at 9 p.m. EDT.Read More
Brenner brand tank trailers will be featured in an episode of the "How It's Made" TV series premiering Thursday, April 9, at 9 p.m. Eastern on the cable Science Channel.Read More
The connection between rubberneckers and those who will watch the "earth-shattering event" promised Dec. 7 by a certain cable network and a man who plans to feed himself to an anaconda.Read More
"The next big TV hit could well be a comedy set in, of all places, a truck stop in Kansas." So writes TruckersNews.com editor David Hollis in the story at this link, following news from several Hollywood media outlets that Big Stop is being produced one of the stars of ABC's Emmy award-winning show Modern Family and is expected to be coming to ABC. No release date has been set nor cast members mentioned. Eric Stonestreet, who plays Cameron Tucker on ...Read More
Here we go again: Following CNBC "Collision Course" series on truck accidents, 20/20 follows with "Road to Ruin" -- more from the public crash/fatigue discussion here.Read More


text:
posted on: 4/28/14
My son is scheduled to take the ACT on June 14, and he needs to achieve a score of 88 to qualify for a scholarship.- ROCHELLE from Oakland, CA
Over the last seven years we've built the largest and most trusted tutoring marketplace on the web. With a new student lead posted every minute, WyzAnt eliminates the need to be listed on multiple sites.
WyzAnt's average commission is just 25% compared to the 60-80% that traditional tutoring companies and franchises take. Sign up for free and set your own rate and schedule!
You don't have to worry about marketing, collecting payment, or unqualified leads on WyzAnt. We take care of all the details so you can focus on tutoring!

subset: commoncrawl


text:
Lemon Cream Cheese Tarts
|Baked tart shells
|6 (3 1/2 Across Top, Fluted)
|Granulated sugar
|2⁄3 Cup (10.67 tbs)
|Lemon juice
|1⁄3 Cup (5.33 tbs)
|Grated lemon rind
|2 Teaspoon
|Softened cream cheese
|9 Ounce (3 Packages, 3 Ounces Each)
|Grape jelly
|6 Teaspoon
Beat eggs until thick and fluffy, in top of double boiler; continue beating while gradually adding sugar, lemon juice, lemon rind.
Cook over boiling water, stirring constantly, till thick; gradually stir into cheese.
Cool; fill shells.
Top with bit of jelly.
Recipe Summary
Rate It
Nutrition Rank
Nutrition Facts
Serving size: Complete recipe
Calories 2568 Calories from Fat 1291
% Daily Value*
Total Fat 145 g223.8%
Saturated Fat 75.5 g377.4%
Trans Fat 0 g
Cholesterol 1059.1 mg353%
Sodium 1519.9 mg63.3%
Total Carbohydrates 288 g96%
Dietary Fiber 8.6 g34.3%
Sugars 207.6 g
Protein 49 g97.7%
Vitamin A 79.6% Vitamin C 83.8%
Calcium 35% Iron 21.3%
*Based on a 2000 Calorie diet


text:
Modern Warfare 2, FIFA 10 were biggest UK PlayStation games in 2009
ELSPA's confirmed that Modern Warfare 2 was the biggest selling game for PlayStation 3, and FIFA 10 for PSP, in the UK last year.
Earlier today, MW2 was heralded as the biggest selling game in the UK on all platforms.
The IW shooter has now pulled in over $1 billion.
On the PS3 side, the top five was finished off by FIFA 10, Assassin's Creed II, Killzone 2 and Uncharted 2: Among Thieves.
For PSP, it was FIFAs 10 and 09 in the top two, followed by Gran Turismo, Ratchet & Clank: Size Matters and LittleBigPlanet.
Full listings below.
PlayStation 3:
1. Call Of Duty: Modern Warfare 2
3. Assassin's Creed II
4. Killzone 2
5. Uncharted 2: Among Thieves
PlayStation Portable:
3. Gran Turismo
4. Ratchet & Clank: Size Matters
5. LittleBigPlanet
Sometimes we include links to online retail stores. If you click on one and make a purchase we may receive a small commission. For more information, go here.


text:
I added some images to a post, and I am trying to display them on the single page. I m using <?php get_children(); ?>, but it is not working. O tried it within the loop and outside of the loop....neither works. Can someone please help me figure out how to fix this?
single.php -- http://wordpress.pastebin.com/B8DKyEgK


text:
A simple category slider with a minimal design using CSS animations and jQuery: http://tympanus.net/codrops/2012/12/31/how-to-create-a-simple-multi-item-slider/
keyboard shortcuts:
L or F like
post comment
Show and tell for designers
What are you working on? Dribbble is a community of designers sharing screenshots of their work, process, and projects.
Copyright © 2009–2016 Dribbble LLC. All screenshots © their respective owners. Shipped from Salem, Mass. USA.


text:
A curated, searchable portal of
inter-related data standards, databases, and policies in the life, environmental and biomedical sciences
Register or update your standard and/or database in our registries; make them more discoverable to other users and get credit for it...[read more]
Find the appropriate standard and database for your dataset. See journal requirements and journal and funding agency data policies...[read more]
Refine your policy discovering which standards and databases are inter-related, more used and mature, and if are funded by you...[read more]
Create your view on an inter-related set of standards and/or databases, a simple way to complement your data guidelines and assist your users...[read more]
Do you have 5-10 minutes to help BioSharing prioritize future enhancements?
Then please fill out this short questionnaire to
prioritise the improvements that are of greatest importance to you.
Your views will help us choose which enhancements to provide first. Thank you very much!


text:
Kenneth S Button
Boston, MA
Also Known As
Get Background Check on Kenneth
Kenneth S Button
Ken S Button
Kenneth S Button is in his 40's
Kenneth Has Lived In
Boston, MA
Doylestown, PA
Kenneth's Relatives
Kenneth S Button
Zodiac SignLeo
StatusPresident at Lawn Depot Inc
StatusPresident at Lawn Depot Inc
Additional Infomation on Kenneth S Button
Kenneth S Button's Contact Information
Known Cities Lived In
Find out where Kenneth S Button has lived as well as Kenneth S Button's phone numbers and email addresses.
Kenneth S Button Has Lived in 3 States
Massachusetts Address for Kenneth S Button
300 A Sx
Boston MA
Boston MA
Has Lived InGet Full Address Report
Boston, MA
Doylestown, PA (7 Addresses)
Phone Numbers Associated with Kenneth S Button
Get Full Phone Report
(215) ***-**** - East Greenville, PA
(215) ***-**** - East Greenville, PA
(800) ***-****
Email Address Associated with Kenneth S Button
Get Email Report
Kenneth S Button's Professional Information
Information regarding Kenneth S Button's professional history. Find out previous places Kenneth S Button has worked as well as dates employed. The following data is not guaranteed for accuracy and should not be used for employment, insurance, credit eligibility, or for any other purpose covered under the Fair Credit Reporting Act.
Kenneth S Button Has Worked at 2 Places
Company: Lawn Depot Inc
Title: President
Company: Circles
Title: Vice President
Kenneth S Button's Experience
Title: President
Company: Lawn Depot Inc
Job Details
The Lawn Depot was founded in March, 1974, in an old book bindery building next to the Doylestown, PA train station. From the start, our stated objective was to help homeowners grow better lawns by explaining the science of "Turf Management" in plain, logical English, and by offering quality lawn and garden maintenance products at reasonable prices. We were the first lawn specialty store in the Central Bucks area. By the early 1980s, our business had outgrown the old bindery building, and we moved The Lawn Depot to our present location in a renovated 100+ year old barn on 8 acres, on Edison-Furlong Rd. in Doylestown Township. Shortly after the move, we broadened the scope of our business to include lawn application services, including fertilization, weed and pesticide application, seeding, and several soil conditioning operations necessary for the establishment and maintenance of healthy turf. Today, our Lawn Depot Turf Management Division maintains over 3000 residential and business lawns in Bucks and Montgomery Counties, with over 60,000,000 sq. ft. of turf under care. Meanwhile, the Lawn Depot Garden Center continues to guide thousands more homeowners who choose to maintain their own lawns and gardens, with the same quality advice and product lines that we've offered for the last 35 years. We are proud of our business and our reputation, and we hope to continue to grow as the Bucks-Mont area grows.
Title: Vice President
Company: Circles
Additional Professional Infomation on Kenneth S Button
All trademarks, product names, company names or logos on this page are the property of their respective owners.
Related People Searches


text:
Capitol Hill Critics Second-Guess Agencies Using...
Co-Sourcing Improves Pharmaceutical Laboratory Productivity
Keep the Opportunities, Lose the Opportunists
Loss of Lives in Clinical Trials...
A naturally occurring line of immunodeficient pigs can support the growth of human tumors injected under their skin, offering a promising new large-animal model for studying human cancers and testing new drugs and treatment strategies. The ability of human melanoma cells and pancreatic carcinoma cells to grow in these pig models is described in an article in BioResearch Open Access, a new bimonthly peer-reviewed open access journal from Mary Ann Liebert, Inc. The article is available free online at the BioResearch Open Access website.
Mathew Basel and colleagues at Kansas State University and Iowa State University highlight the advantages that pig disease models offer, as they are anatomically and physiologically more closely related to humans than traditional rodent animal models. As a result, findings from studies in large animal models such as pigs are more likely to translate into similar outcomes in humans. The authors present their findings in the article "Human Xenografts Are Not Rejected in a Naturally Occurring Immunodeficient Porcine Line: A Human Tumor Model in Pigs."
In the study, wild-type and immunodeficient pigs were injected subcutaneously in the left ear with human melanoma cells (A375SM cells) and in the right ear with human pancreatic carcinoma cells (PANC-1). All immunodeficient pigs developed tumors that were verified by histology and immunohistochemistry. Nonaffected littermates did not develop tumors.
"This novel animal model has the potential to become a highly useful model in cancer research studies, in addition to providing significant opportunities for drug discovery and other translational applications," says Editor-in-Chief Jane Taylor, Ph.D., MRC Centre for Regenerative Medicine, University of Edinburgh, Scotland. Immunodeficient pigs, which do not reject xenografted human tumors, have the potential to become an extremely useful animal model for cancer therapy because of their similarity in size, anatomy, and physiology to humans.
To save this item to your Favorites,
log into GEN Select.
We'll be sure to take you back here after you do.
Not a member of GEN Select?
To comment on this article,
log into GEN Select.
We'll be sure to take you back here after you do.
Join 110,000 colleagues who rely on GEN Highlights for breaking news and exclusive articles shaping today's life science tools and technologies.
You're all set! Thank you for subscribing to GEN Highlights.
MDMA (commonly known as the empathogen "ecstasy") is classified as a Schedule 1 drug, which is reserved for compounds with no accepted medical use and a high abuse potential. Two researchers from Stanford, however, call for a rigorous scientific exploration of MDMA's effects to identify precisely how the drug works, the data from which could be used to develop therapeutic compounds.Do you agree that ecstasy should be studied for its potential therapeutic benefits?
Be sure to add [email protected]
to your Address Book or Safe Senders List.
here for instructions.
If you have any questions about your subscription, click
here to email us or call at (914) 740-2189.
You may also be interested in subscribing to the GEN magazine, an indispensable
resource for everyone involved in the business of translating discoveries at the
bench into solutions that fight disease and improve health, agriculture, and the
environment. Subscribe
today to see why over 60,000 biotech professionals read GEN to
keep current in the areas of genomics, proteomics, drug discovery, biomarker discovery,
bioprocessing, molecular diagnostics, collaborations, biotech business trends, and
© 2016 Genetic Engineering & Biotechnology News All Rights Reserved


text:
Lamb Casserole
Total Time
Prep 20 mins
Cook 2 hrs
This is an adaptation of a lamb shank recipe I found in a magazine. I don't know the name of the magazine and I have made a few changes. This is great on a cold winters night.
Ingredients Nutrition
- 1 kg lamb (diced)
- 1⁄4 cup plain flour
- 1 tablespoon olive oil
- 2 cloves garlic (crushed)
- 3⁄4 cup dry white wine
- 2 beef bouillon cubes, dissolved in
- 3⁄4 cup hot water
- 2 (440 g) cans diced tomatoes
- 1 (45 g) can anchovy fillets (diced well)
- 1 medium carrot (diced)
- 1 medium onion (diced)
- 1⁄2 teaspoon dried chili pepper flakes or 2 fresh chili peppers
- 1 sprig fresh basil
- 6 olives (diced well)
- 1 tablespoon balsamic vinegar
- salt and pepper, to taste
- Toss diced lamb in plain flour with salt& pepper.
- Heat oil in large frypan and cook lamb until brown.
- Remove from pan and set aside.
- Add garlic, onion, carrots, wine and bring to boil.
- Add beef stock, tomatoes, anchovies, chilli and basil, stir to combine.
- Return lamb to dish and bring to boil.
- Cover frypan and reduce heat to med-low (if it starts to stick to the bottom of the pan the temp is too high) and cook for 2 to 2 1/2 hours, stirring occasionaly.
- When cooked add diced olives and balsamic vinegar and simmer for 5 minutes.
- Serve with a side of your choice.
- My husband likes this served with mashed potato and green beans.
Most Helpful
This was AMAZING. I added a few vegetables, and some Yukon Gold potatoes, but it was really really scrumptious. Loved it and my boyfriend thought it was "Top Notch".
This is the very best lamb casserole dish I have made. Everyone loved it and they are a fussy lot! Fantastic recipe. Thank you!


text:
Welcome, skip to the content.
Explore Melbourne's history through stories of people from across the world who have migrated to Victoria at the Immigration Museum. From the reasons for making the journey, to the moment of arrival in a new country, and the impact on indigenous communities - these stories are sometimes sad, sometimes funny, but always engaging. The result is a thought-provoking and moving experience.Take a fresh look at what it means to belong and not belong in Australia. Explore who Australians are and who others think Australians are.
The Immigration Museum is just a 10 minute walk from Federation Square and Flinders Street Station.
Closed Christmas Day and Good Friday


text:
Blonde and Blonder is a 2008 Canadian comedy film starring Pamela Anderson, Denise Richards and Emmanuelle Vaugier. The film was directed by Dean Hamilton, and was released on January 18, 2008. Wikipedia
Dawn St. Dom: I got fired for typos. Dee Twiddle: What are typos? Dawn St. Dom: I have no idea...
|2 points
|1 point


text:
Roberts, David L
School of Medicine
Dr. Roberts is a clinical psychologist and Clinical Director of the Transitional Care Clinic. He conducts individual and group psychotherapy for people with psychosis, depression and other difficulties. Roberts conducts research on the effectiveness of psychotherapy and other mental health services. He also studies social cognition and delusions. Roberts teaches psychotherapy and other psychosocial intervention approaches to students and clinicians. PDF Curriculum Vitae
|10/2014 - Present
|Clinical Director
|University of Texas Health Science Center at San Antonio, Psychiatry, San Antonio, TX
|8/2010 - Present
|Assistant Professor
|The University of Texas Health Science Center at San Antonio, Psychiatry, San Antonio, TX
|Postdoctoral Fellowship
|Yale University School of Medicine
New Haven , CT
|Clinical Psychology
|University of North Carolina at Chapel Hill
Chapel Hill , NC
|Clinical Psychology
|Yale University School of Medicine
New Haven , CT
|Clinical Psychology
|University of North Carolina at Chapel Hill
Chapel Hill , NC
|Social Sciences Curriculum
|University of Chicago
Chicago , IL
|Anthropology (Honors)
|Wesleyan University
Middletown , CT
|# Students
|11/2013 - Present
|Psychology Residency Program Didactic
|10 students
|2.5 hour seminar on psychosocial treatments for schizophrenia
|1/2012 - Present
|Post Graduate Rotation Supervision
|11/2011 - Present
|Psychology Internship Training Program
|South Texas Veterans Health Care System
|4 students
|1 hour seminar on assessment in psychotherapy and 1 hour seminar on incorporating research into psychology careers
|11/2011 - Present
|Psychology Postdoctoral Training Program I
|South Texas Veterans Health Care System
|4 students
|1 hour seminar on publishing in psychology and 1 hour seminar on grant writing
|11/2011 - Present
|Psychology Internship Training Program
|San Antonio State Hospital
|3 students
|2 hour seminar on psychosocial treatments for schizophrenia
|1/2009 - Present
|Post Graduate Rotation Supervision
|FEGS Health and Human Services System, New York
Journal Article
|Buck BE, Healey KM, Gagen EC, Penn DL, Roberts DL. Social Cognition in Schizophrenia: Factor structure, clinical and functional correlates Journal of Mental Health 2016 Jan;:1-8.
|Velligan DI, Roberts DL, Sierra C, Fredrick M. What patients with severe mental illness transitioning from hospital to community have to say about care and shared decision making Issues in Mental Health Nursing 2016 Jan;.
|Velligan DI, Roberts DL, Mintz J, Maples NJ, Li X, Medellin E, Brown M. A randomized pilot study of MOtiVation and Enhancement training (MOVE) for negative symptoms in schizophrenia Schizophrenia Research 2015 May;165(2-3):175-180.
|Healey KM, Combs DR, Gibson CM, Keefe RS, Roberts DL, Penn DL. Observable Social Cognition--A Rating Scale: An interview-based assessment for schizophrenia Cognitive Neuropsychiatry 2015 Jan;20(3):198-221.
|Velligan DI, Tai S, Roberts DL, Maples NJ, Brown M, Mintz J, Turkington D. A randomized controlled trial comparing cognitive behavior therapy, cognitive adaptation training, their combination and treatment as usual in chronic schizophrenia Schizophrenia Bulletin 2015 Jan;41(3):567-603.
|Roberts DL, Combs D, Willoughby M, Mintz J, Marks-Gibson C, Rupp B, Penn DL. A randomized, controlled trial of Social Cognition and Interaction Training (SCIT) for outpatients with schizophrenia spectrum disorders British Journal of Clinical Psychology 2014 Sep;53(3):281-98.
|Kanie A, Hagiya K, Ashida S, Pu S, Kaneko K, Mogami T, Oshima S, Motoya M, Niwa S, Inagaki A, Ikebuchi E, Kikuchi A, Yamazaki S, Iwata K, Roberts DL, Nakagome K. A new instrument for measuring multiple domains of social cognition: Construct validity of the Social Cognition Screening Questionnaire (Japanese version) Psychiatry and Clinical Neurosciences 2014 Sep;68(9):701-11.
|Liefland L, Roberts DL, Ford R, Stevens BJ. Depressive Symptoms Among Help-Seeking Latinas in a Disadvantaged, Urban, Northeastern Community Mental Health Center Community Ment Health J 2014 Apr;50(3):331-335.
|Hasson-Ohayon I, Mashiach-Eizenberg M, Avidan M, Roberts DL, Roe D. Social Cognition and Interaction training: Preliminary results of a RCT study in a community setting in Israel Psychiatric Services 2014 Apr;65(4):555-558.
|Velligan DI, Maples NJ, Roberts DL, Medellin E. Integrated psychosocial treatment for negative symptoms American Journal of Psychiatric Rehabilitation 2014 Jan;17:1-19.
|Walss-Bass C, Fernandes JM, Roberts DL, Service H, Velligan D. Differential correlations between plasma oxytocin and social cognitive capacity and bias in schizophrenia Schizophrenia Research 2013 Jul;147(2-3):387-392.
|Xu Z Roberts DL Wang Y Shen Z Cao R. Social cognition and interaction training in patients with schizophrenia: An ERP study Chinese Journal of Nervous and Mental Diseases 2013 Jan;.
|Wang Y, Roberts DL, Xu B, Cao R, Yan M, Jiang Q. Dissociation of understanding from applying others false beliefs in remitted schizophrenia: Evidence from a referential communication task BMC Psychiatry 2013 Jan;13(141).
|Wang Y, Roberts DL, Xu B, Cao R, Yan M, Jiang Q. Social cognition and interaction training for patients with stable schizophrenia in Chinese community settings Psychiatry Research 2013 Jan;13(141).
|Roberts DL, Velligan DI. Can social functioning in schizophrenia be improved through targeted social cognitive intervention? Rehabilitation Research and Practice 2012 Jun;2012.
|Roberts DL, Kleinlein, P, Stevens, BJ. An alternative to generating alternative interpretations in social cognitive therapy for psychosis Behavioural and Cognitive Psychotherapy 2012 Jan;.
|Roberts DL, Velligan DI. Medication adherence in schizophrenia Drug Discovery Today: Therapeutic Strategies 2011 Dec;8(1-2):11-15.
|Roberts DL, Fredrick MM, Carr HN, Velligan DI. Interventions to improve oral medicaion adherence in psychosis: The role of adherence measurement Neuropsychiatry 2011 Jan;1:361-369.
|Roberts DL, Penn DL, Corrigan P, Lipkovitch I, Kinon B, Black RA. Antipsychotic medication and social cue recognition in chronic schizophrenia Psychiatry Research 2010 Jan;178:46-50.
|Roberts DL, Penn DL, Labate D, Margolis S, Sterne A. Transportability and feasibility of Social Cognition and Interaction Training (SCIT) in community settings Behavioural & Cognitive Psychotherapy 2010 Jan;38:35-47.
|Roberts DL, Penn DL. Social Cognition and Interaction Training (SCIT) for outpatients with schizophrenia: A preliminary study Psychiatry Research 2009 Jan;166:141-147.
|Roberts DL, Penn DL. The effects of task engagement and interpersonal rapport on WCST performance in schizophrenia American Journal of Psychiatric Rehabilitation 2009 Jan;12:57-72.
|Couture SM, Penn DL, Roberts DL. The functional significance of social cognition in schizophrenia: A review Schizophrenia Bulletin 2006 Jan;32:44-63.
|Couture SM, Roberts DL, Penn DL, Cather C, Otto M, Goff DC. Do baseline client characteristics predict the therapeutic alliance in the treatment of schizophrenia? Journal of Nervous and Mental Disease 2006 Jan;194:10-14.
|Roberts DL, Penn DL, Cather C, Otto M, Goff DC. Should CBT target the social impairments associated with schizophrenia? Journal of Cognitive Psychotherapy: An International Quarterly 2004 Jan;18:255-264.
|McCoy ML, Roberts DL, Hanrahan P, Clay R, Luchins DJ. Jail linkage ACT services for individuals with mental illnesses Psychiatric Rehabilitation Journal 2004 Jan;27:243-250.
|Hanrahan P, Luchins DJ, Savage C, Patrick G, Roberts DL, Conrad KJ. Representative payee programs for mentally ill persons in Illinois: Census survey Psychiatric Services 2002 Jan;53:190-194.
|Roberts DL, Penn DP. Social Cognition in Schizophrenia: From Evidence to Treatment New York, NY: Oxford University Press; 2013.
|Roberts DL, Penn DL, Combs DR. Social Cognition and Interaction Training [Japanese translation] Tokyo, Japan: Seiwa Shoten Publishers; 2011. 154 p.
Book Chapter
|Roberts DL, Pinkham AE. The future of social cognition in schizophrenia: Implications from the normative literature. In: Roberts DR, Penn DL. Social Cognition in Schizophrenia: From Description to Treatment. New York, NY: Oxford University Press; 2012.
|Roberts DL. Alien intentionality in schizophrenia. In: Mishara A, Schwartz M, Corlett P, & Fletcher P. Phenomenological Neuropsychiatry: Bridging the Clinic with Clinical Neuroscience. New York, NY: Springer Science; 2012.
|Roberts DL, Pinkham AE, Penn DL. Group cognitive behavioral therapy for schizophrenia. In: Bieling PJ, Anthony M, McCabe R. Cognitive Behavioral Groups: Skills and Processes. New York: Guilford Publications; 2006.
|Roberts DL, Diggins L, Parente L, Fiszdon J. Understanding social situations (USS): Development of a new social cognitive intervention for individuals with psychosis; 2014 Apr. (Schizophrenia Research; vol. 153, no. 1).
|Roberts DL, Walss-Bass C, Velligan DI, Carr H. Social cognition, self regulation and neurohormones in schizophrenia; 2013 May. (Schizophrenia Bulletin; vol. 39, no. S1).
|Roberts DL. Automatic and controlled processes in social cognition training; 2012 Apr. (Schizophrenia Research; vol. 136, no. 1-3).
|Roberts DL, Fiszdon J, Tek C. Initial validity of the Social Cognition Screening Questionnaire (SCQC); 2011 Jan. (Schizophrenia Bulletin; vol. suppl, no. 1).
|Funding Agency
|Patient-Centered Outcomes Research Institute
|Improving transitional care experiences for individuals with serious mental illness
|10/2013 - 10/2016
|Grant Detail
|Funding Agency
|Patient-Centered Outcomes Research Institute (PCORI)
|Improving transitional care experience for individuals with serious mental illness
|9/2013 - 8/2016
|Grant Detail
|This grant is designed to investigate patient centered outcomes through comparing the triage group vs. standard intake appointment processes at the Transitional Care Clinic. The clinic serves post-acute psychiatric patients discharged from inpatient units and emergency rooms. The additional treatment component of shared decision making will be offered to participants whom are randomized to the group triage process. Outcomes will be assessed based upon patient satisfaction and attainment of treatment goals.


text:
Opensuse Build Service 1.0: Open Teamwork
The Novell sponsored Opensuse project has released version 1.0 of its build service in a step that will make working on software packages for the distribution much easier for external developers.
Developers can create a branch of the official factory package, use the build service to create a version and hand in the results to the package's official maintainer. If the maintainer does not have time to consider the package, it does not become obsolete, but is referenced by future builds.
"This is the first time that external developers have been able to collaborate with us on the distribution by sending package changes to maintainers", says Suse employee Klaas Freitag, and Build Service architect, explaining the changes. To help support communications in this collaborative environment, the build service also has new submission and notification functions.
The updated Build Service runs on the build.opensuse.org website. The GPL-licensed source code is available from Novell Forge. Binary packages for various Suse versions are also available.
Issue 26: Linux Shell Handbook 7th Ed./Special Editions
Buy this issue as a PDF
Mirantis to Refactor OpenStack Fuel for Kubernetes
The company is collaborating with Google and Intel to use Kubernetes as an engine for Fuel
Canonical Joins Document Foundation Advisory Board
The upcoming release of LibreOffice will be available as a snap package
SUSE Joins the Microsoft Test Drive Program
Customers can take a free test drive of SLES for HPC on the Azure Cloud
SiFive Launches a Line of Open Source Chips
San Francisco-based chip company announces their first fully open source chip platform.
Fedora 24 Released
The whole distro gets rebuilt on glibc 2.3
Canonical Announces Cross-Distro Support for Snaps
Ubuntu Vendor tries to solve app packaging and distribution problem across distributions.
ownCloud Forked
Founder of ownCloud launches the Nextcloud project.
HP Enterprise Unveils a Futuristic Computer
Will The Machine change the way future programmers think about memory?
CoreOS Announces Distributed Storage System
The new Torus distributed storage system is available under an open source license on GitHub
Google Beats Oracle in the Android Lawsuit
Juries decides Google's use of Java APIs Was Fair Use


text:
Getting firsthand information from an actual car owner is one sure way to get an idea of car performance once it leaves the lot. Autobytel features real consumer reviews that give the unbiased experience of a car.
It was a good. Car
Pros: Mercedes is the safest automobile on the road!
Cons: The navigation systemFast, agiile, comfortabe, economical, handsome styling with every ergonomic creature comfort available. That sums up the E-350 Mercedes. Combined with the 4matic transmission it becomes the perfect car for the Pacific Northwest. A consistent 26 mpg on the road and 19-21 around town makes this luxury car competitive with the Accord and similar models. The nimble feel and smooth ride combine to make this a viable sports-sedan for drivers like me. The 4matic never lets me down in winter driving, and provides tiger-like grip on rain soaked roads or patches of gravel. The interior is perhaps the most attractive passenger cabin in all of automotive history. The silver exterior is complemented by the dove gray leather of the interior. Heated and cooled seats is a touch of pampering not found in earlier Mercedes automobiles and is most appreciated. The navigation system is a bit more complex that those found on some other makes of automobiles, but with a short course on the MB system is is both useful and accurate. In sum, the E350 is the perfect balance in automotive engineering.
great car
Best vehicle ever
Pros: Overall ride and handling -- and its a great looker also.
Cons: power window button for the driver's side window is awkward to accessHave owned BMW M3 for nine years -- but this M-B is tops in my book. It cruises the highway at 2000 rpm (doing 77 mph) and still has the torque to get up and go higher from there. The ride is firm and reassuring. I'm disappointed that M-B has discontinued making the CLK.
2006 CLK350 Cabriolet
Pros: Black rag top with Capri blue body and stone interior
Cons: wish it was the v8I bought this car used and it is smoother and quieter than any new car I have been in. The lines are beautiful the car accelerates great and I have not had any trouble with it.
Pros: Diesel power, fuel mileage, excellent handling, ride.
Cons: Expense to maintain, otherwise nothing.Purchased this E320 used from a dealer in Virginia. No regrets. Best car we have owned. The only reason I did not give it 5 stars was the fact that it is not owner serviceable and no oil dipstick; otherwise it is perfect. Got used to the navigation and love it.
Pros: the diesel engine
Cons: lumbar support
Advice: enjoygreat combination vehicle. good at many things, great at none


text:
Who We Are
What We Do
Board of Directors
Community Partners
2016 Individual Donors
Contact Us
Subscribe to Our Mailing List
Ways to Help
Youth Volunteers (14-17 years old)
Become a Mentor
Mentoring Interest Form
Group Volunteer Proposal
Volunteer Application
Volunteer Information Video
Join our Board of Directors
Summer Camp 2016
Afterschool Programs
Inspiration Celebration
Clubs for the Clubs Golf Tournament
The Great Pumpkin Glow 2015
Employment Opportunities
Internship: Executive Assistant
Education Program Leader- Medford Club
Program Leader
Email / Username:
Remember me.
Forgot your password?
Join us for a fun-filled six weeks of summer camp at our Medford Boys & Girls Club. Activities include daily outings, recreational games, sports, arts, computers, library visits, and weekly field trips such as Boston Harbor Islands, Launch Trapmoline Park, and Canobie Lake Park.
Vouchers accepted.
Check out our
Summer Camp
page for more information and application!
Support a camper today!
Summer Camp
1 Child 1 Week $175.00 USD
1 Child - Full Campership $1,080.00 USD
Thank you for giving to the Boys and Girls Clubs of Middlesex County
Copyright 2016, Boys & Girls Clubs of Middlesex County, All Right Reserved.


text:
Former "American Idol" judge Simon Cowell says he's happy that Steven Tyler will take his place.
Cowell says, "I always said it had to be someone who wasn't better looking than me and they achieved it."
He poked fun at Tyler before collecting the Founders Awards at the International Emmy Awards in New York on Monday night.
Cowell said the Aerosmith frontman seems like a nice guy, and Randy Jackson told him they were having a lot of fun.
He believes Tyler can adequately fill his shoes, but stopped short of saying the rock star would damage his credibility being on the show.
Cowell says the role of judge is something that Tyler wants to do and that "it's a fun job. You got to take it like that. You can't take it too seriously."
Cowell :Tyler Perfect 'Idol' Replacement
© 2010 The Associated Press. All Rights Reserved. This material may not be published, broadcast, rewritten, or redistributed.


text:
Rob Clement
Photography has always been an interest of mine. In college, while taking an intro Photography course I realized that I was seeing the world around me through a rectangle, even without my camera. The bug had bitten and bitten hard.
I later enrolled in Brooks Institute of Photography where I graduated with honors. Then I took over the photo and art dept of the Ojai Valley News where I won several State and one National Award for the paper.
I currently run my freelance business, RCVisual, from Denver with my wife Stephanie, daughter Kiya and son Keenan.
Nikon D700 24-70mm Nikkor f/1.4 50mm Prime Nikkor SB-800 and SB-600 (and a bunch of other toys, too!)
49 answers
10 questions
~119k people reached
- Denver, CO
- rcvisualarts.com
- Member for 5 years, 10 months
- 442 profile views
- Last seen Oct 17 '15 at 16:20
Top Posts (59) All Questions Answers | Votes Newest
16 What are the pros and cons of using a watermark? May 9 '13
15 Reasons to use in camera special effects? Oct 12 '10
14 What is the best techinque to smooth skin? Sep 29 '10
10 Shooting Corporate Brass, am I forgetting anything Nov 17 '10
8 How do I choose a bridge camera? Sep 21 '10
Badges (35)
Gold —
Silver 9
Bronze 26


text:
Telephone: 612-624-8970; Fax: 612-626-4411
Translational and Clinical Research
A Fibrin Patch-Based Enhanced Delivery of Human Embryonic Stem Cell-Derived Vascular Cell Transplantation in a Porcine Model of Postinfarction Left Ventricular Remodeling†‡§
Version of Record online: 24 FEB 2011
DOI: 10.1002/stem.580
Copyright © 2010 AlphaMed Press
Volume 29, Issue 2, pages 367–375, February 2011
Additional Information
How to Cite
Xiong, Q., Hill, K. L., Li, Q., Suntharalingam, P., Mansoor, A., Wang, X., Jameel, M. N., Zhang, P., Swingen, C., Kaufman, D. S. and Zhang, J. (2011), A Fibrin Patch-Based Enhanced Delivery of Human Embryonic Stem Cell-Derived Vascular Cell Transplantation in a Porcine Model of Postinfarction Left Ventricular Remodeling. STEM CELLS, 29: 367–375. doi: 10.1002/stem.580
- †
Author contributions: Q.X.: data collection and/or assembly of data, data analysis and interpretation, manuscript writing; K.L.H.: data collection and/or assembly of data, data analysis and interpretation; Q.L.: data collection and/or assembly of data, data analysis and interpretation; P.S.: data collection, data analysis and interpretation; A.M.: data collection and/or assembly of data; X.W.: data collection and/or assembly of data, data analysis and interpretation; M.N.J.: data collection and/or assembly of data; P.Z.: data collection and/or assembly of data, data analysis and interpretation; C.S.: data collection and/or assembly of data, data analysis and interpretation; D.S.K.: conception and design, financial support, administrative support, assembly of data, and final approval of manuscript; J.Z.: conception and design, financial support, administrative support, assembly of data, data analysis and interpretation, manuscript writing, final approval of manuscript.
- ‡
Disclosure of potential conflicts of interest is found at the end of this article.
- §
First published online in STEM CELLSEXPRESS December 23, 2010.
Publication History
- Issue online: 24 FEB 2011
- Version of Record online: 24 FEB 2011
- Accepted manuscript online: 23 DEC 2010 01:21PM EST
- Manuscript Accepted: 20 NOV 2010
- Manuscript Received: 11 JUN 2010
Funded by
- U.S. Public Health Service Grants. Grant Numbers: HL50470, HL67828, HL 95077, HL100407, HL077923
- Engdahl Family Foundation
- Stem cells;
- Myocardial infarction;
- Heart failure
- Top of page
- Abstract
- Acknowledgements
- Supporting Information
It is unknown how to use human embryonic stem cell (hESC) to effectively treat hearts with postinfarction left ventricular (LV) remodeling. Using a porcine model of postinfarction LV remodeling, this study examined the functional improvement of enhanced delivery of combined transplantation of hESC-derived endothelial cells (ECs) and hESC-derived smooth muscle cells (SMCs) with a fibrin three-dimensional (3D) porous scaffold biomatrix. To facilitate tracking the transplanted cells, the hESCs were genetically modified to stably express green fluorescent protein and luciferase (GFP/Luc). Myocardial infarction (MI) was created by ligating the first diagonal coronary artery for 60 minutes followed by reperfusion. Two million each of GFP/Luc hESC-derived ECs and SMCs were seeded in the 3D porous biomatrix patch and applied to the region of ischemia/reperfusion for cell group (MI+P+C, n = 6), whereas biomatrix without cell (MI+P, n = 5), or saline only (MI, n = 5) were applied to control group hearts with same coronary artery ligation. Functional outcome (1 and 4 weeks follow-up) of stem cell transplantation was assessed by cardiac magnetic resonance imaging. The transplantation of hESC-derived vascular cells resulted in significant LV functional improvement. Significant engraftment of hESC-derived cells was confirmed by both in vivo and ex vivo bioluminescent imaging. The mechanism underlying the functional beneficial effects of cardiac progenitor transplantation is attributed to the increased neovascularization. These findings demonstrate a promising therapeutic potential of using these hESC-derived vascular cell types and the mode of patch delivery. STEM CELLS 2011;29:367–375
- Top of page
- Abstract
- Acknowledgements
- Supporting Information
Myocardial infarction (MI) often induces a period of left ventricular (LV) remodeling. When LV remodeling occurs, an initial period of hemodynamic stability is followed by the development of LV dysfunction that may eventuate in congestive heart failure (CHF). The mechanisms that contribute to the transition from the compensated state to CHF remain unclear but may be related to progressive contractile dysfunction of the border zone (BZ) region of viable myocardium that surrounds the infarct [1, 2].
Both experimental and clinical evidence demonstrate that cellular transplantation can improve the LV contractile performance of failing hearts [3–7]. The underlying mechanisms remain unclear. Transplanted cells may regenerate myocytes and new vessels, and they may also release cytokines that exert trophic effects on host cardiac cells. We hypothesize that the beneficial effects of BZ stem cell engraftment result in increased neovascularization in the infarct zone (IZ) and possibly BZ and paracrine effects on stressed native cardiomyocytes in the BZ. This results in stabilization of BZ bioenergetic and contractile function, which in turn is associated with attenuated myocyte apoptosis and expansion of the BZ size.
Although it is a consistent observation in the literature that cellular transplantation improves LV contractile function, the cell engraftment rate a few weeks after the transplantation is usually very low [8–12]. Therefore, it is clear that majority of cells transplanted to the heart do not demonstrate durable engraftment. Further, the majority of transplanted cells that do engraft do not differentiate into host cardiac myocytes cell phenotypes [6, 11–13].
We have recently developed a novel three-dimensional (3D) porous fibrin biomaterial that can bind to growth factors to create an optimal microenvironment for stem cells to reside [7, 14–16]. The biomaterial can also function to control prolonged release of growth factors (stromal cell derived factor-1 alpha [SDF-1α]) to mobilize the endogenous cardiac progenitors to the injury site enhancing the repair. Using swine and mouse models of hearts with acute myocardial infarction, we have recently shown that bone marrow-derived mesenchymal stem cells (MSCs) embedded in a novel 3D porous biomaterial "patch" that attached to the surface of the myocardial infarction, resulted in a remarkable increase of engraftment rate and significant functional improvements a few weeks after transplantation [7, 14–16].
Additionally, we have recently developed a novel method in differentiating human embryonic stem cell (hESC) into endothelial cells (hESC-ECs) and smooth muscle cells (hESC-SMCs), which can provide an ample source for clinical cellular therapy application in patients with heart failure [17].
In this study, we hypothesized that the trophic effects of cellular therapy are associated with increased neovascularization and regional myocardial contractile function, reduction of LV wall stresses and myocytes apoptosis, and possibly mobilization of the endogenous cardiac progenitors to the injury site for repair. Using a well-established clinically relevant pig model of postinfarction LV remodeling, and an enhanced cell delivery by the 3D porous fibrin biomatrix, we examined whether the transplantations of mixture of hESC-ECs and hESC-SMCs can ameliorate LV dysfunction and hypertrophy in hearts with postinfarction LV remodeling. Immunosuppression was achieved by using the established xeno-transplantation protocol. LV infarct size and chamber function was examined by magnetic resonance imaging (MRI). Additionally, we used an immunodeficient mouse model of postinfarction LV remodeling to assess the time course of the in vivo cell engraftment rate using molecular bioluminescent imaging (BLI). The results demonstrated that the fibrin patch enhanced delivery ofhESC-ECs and hESC-SMCs resulted in a significant cell engraftment, which is accompanied by improvement of LV chamber function, reduction of infarct size, and increase of neovascularization at both infarct zone and peri-infarct BZ, suggesting a promising novel cellular therapy using hESC-derived vascular cells with the novel biomatrix delivery mode.
- Top of page
- Abstract
- Acknowledgements
- Supporting Information
All experiments were performed in accordance with the animal use guidelines of the University of Minnesota, and the experimental protocols were approved by the University of Minnesota Research Animal Resources Committee. The investigation conformed to the Guide for the Care and Use of Laboratory Animals published by the National Institutes of Health (NIH publication No. 85-23).
ESC Culture and Vascular Differentiation
Human embryonic stem cell line H9 (Wicell, Madison, WI) was maintained on mouse embryonic fibroblasts and genetically modified to express green fluorescent protein and firefly luciferase (GFP/Luc), as previously described [18]. Endothelial cells (hESC-ECs) and smooth muscle cells (hESC-SMCs) were derived from the H9 GFP/Luc cell line as previously described and cultured under EC and SMC conditions, respectively [17]. Briefly, undifferentiated hESCs were cocultured with M210 mouse stromal cells to induce mesoderm differentiation. After 13–15 days, CD34+CD31+ hESC-derived vascular progenitor cells were sorted by magnetic nanoparticle selection system. Cells were cultured on fibronectin-coated plates with cytokine containing media to support EC development. Subsequently, to generate SMCs, these cells were cultured in media containing platelet-derived growth factor-BB and transforming growth factor beta. Immediately prior to transplantation, cells were harvested using 0.05% Trypsin-1 mM EDTA (Invitrogen, Carlsbad, CA), counted, pelleted, and resuspened in 30 μl phosphate-buffered saline solution (Hyclone/Thermo Scientific, Waltham, MA) for mouse studies or in 1 ml fibrinogen solution (Sigma-Aldrich, St. Louis, MO) for pig studies.
Mouse Studies
Immunodeficient nonobese diabetic/severe combined immune deficiency mice approximately 12 weeks of age weighing 17–20 g were used for the infarct model. Acute myocardial infarction (AMI) was induced by left coronary artery ligation according to a previously described method [19]. Fifteen minutes post ligation of left anterior descending (LAD) coronary artery, mice were randomized into three groups that received saline only (MI, n = 13), hESC-derived ECs (MI+EC, n = 8), or a mixture of hESC-derived ECs and SMCs (MI+EC+SMC, n = 13). Cell transplantation was achieved with three injections (total volume of 30 μl) into the peri-infarct regions with a 31-gauge needle. A total of 1 × 106 cells (100% hESC-EC or a 50/50 mixture of hESC-EC and hESC-SMC) were delivered to MI+EC and MI+EC+SMC groups, respectively. The functional outcome of cell transplantation was assessed by echocardiography [10] with 4-week follow-up, whereas in vivo cell engraftment rate was measured with BLI [20]. Following the 4-week echocardiogram, mice were sacrificed and the hearts excised. Mouse heart infarct size was measured by using NIH Image J software and saved tissue was subject to cryosection and immunofluorescent staining [10]. Methods detailing mouse AMI surgery, cardiac echocardiography, in vivo BLI, measurement of infarct size, and immunohistochemistry are described in the Supporting Information Materials.
Swine Model of Myocardial Ischemia/Reperfusion
Details of the animal model of postinfarction LV remodeling secondary to myocardial ischemia/reperfusion have been described previously [7, 11]. Briefly, young Yorkshire female swine (∼20kg; Manthei Hog Farm, Elk River, MN) were anesthetized with pentobarbital (30 mg/kg, intravenous [i.v.]), intubated, and ventilated with a respirator with supplemental oxygen. A left thoracotomy was performed. The root of first diagonal coronary artery from LAD coronary artery was ligated for 60 minutes followed by reperfusion to create MI, which resulted in 10% LV mass damage. Other drugs administrated during open-chest surgery include Lidocaine (2 mg/kg i.v. bolus before ligation followed by 1 mg kg−1 min−1 i.v. for 70 minutes) and nitroglycerine (0.5 g kg−1 min−1) i.v. for 70 minutes starting 10 minutes before ligation). If ventricular fibrillation occurred, electrical defibrillation was performed immediately. Fifteen minutes after reperfusion, surviving pigs were randomized to ligation only (MI,n = 5), open fibrin patch transplantation (MI+P, n = 5), and cell-seeded fibrin patch transplantation (MI+P+C, n = 6) groups. The chest was then closed. Animals received standard postoperative care including analgesia until they ate normally and became active.
Fibrin Patch-Based Stem Cell Transplantation
A fibrin patch was employed as the vehicle to deliver stem cell as described previously in detail [7] (Supporting Information Materials).
All pigs received clinical protocol of immunosuppression for xeno-transplantation with Cyclosporine 30 mg kg−1 per day with food as previously described in details [12].
MRI Methods
MRI was performed on a 1.5 Tesla clinical scanner (Siemens Sontata, Siemens Medical Systems, Islen NJ) using a phased-array 4-channel surface coil and ECG gating as previously described in details [21] (Supporting Information Materials).
Tissue Preparation and Immunohistochemistry
After the MRI experiments were completed, the animals were anesthetized, the thoracotomy incision was reopened. Hemodynamics was measured. The heart was then explanted. The LV was sectioned in a bread-loaf manner into six transverse sections (1 cm in thickness) from apex to base. Detailed immunohistochemistry methods have been described previously [9, 10] (Supporting Information Materials).
Analysis of Myocardial Vascular Density
Vascular density was assessed using the methods as previously described in details [11] (Supporting Information Materials).
Statistics and Data Analysis
The repeated measures ANOVA was applied to compare the measurements of systolic thickening fraction, ejection fraction (EF), and scar size across treatment groups and different time points (before MI, 1 week, and 4 weeks after MI). The significance level of type I error (p < .05) was used. The Bonferroni correction for the significance level was used to take into account multiple comparisons [22]. All values are expressed as mean ± SD. All statistical analyses were performed in Sigmastat version 3.5 (San Jose, CA).
- Top of page
- Abstract
- Acknowledgements
- Supporting Information
Endothelial and Smooth Muscle Cells Derived from Human ESCs
As previously demonstrated, hESCs cocultured with stromal cells gave rise to a population of CD34+CD31+ cells [23]. These hESC-derived CD34+CD31+ cells function as vascular progenitor cells that can be placed into secondary culture systems to produce homogeneous populations of endothelial and smooth muscle cell types (hESC-ECs and hESC-SMCs), respectively [17]. Here, we derived hESC-ECs and hESC-SMCs from the H9 hESC line that has stable expression of GFP and luciferase (GFP/Luc). These hESC-ECs exhibit classic endothelial cell morphology, express endothelial surface markers, and form capillary structures on Matrigel (Fig. 1A). hESC-SMCs also assumed appropriate morphology, expressed intracellular smooth muscle cell markers, and lacked expression of endothelial surface marker CD31 (Fig. 1B).
Significant Improvement of Cardiac Function and Cell Engraftment in Infarcted Mouse Hearts Treated with hESC-ECs and hESC-SMCs
We have previously demonstrated interaction between hESC-ECs and hESC-SMCs in vitro [17]. Data from present study demonstrated these cell populations significantly improved LV chamber function and regional myocardial contractile performance in vivo while transplantation of hESC-ECs alone showed moderate improvement when compared with hearts that did not receive a cellular treatment and exposed to an identical LAD ligation protocol (Fig. 2A, 2B). As the hESC-derived cells maintained stable Luc expression, we were able to use BLI to demonstrate persistence of the hESC-derived cells up to 8 weeks post-treatment (Fig. 2C–2E). BLI illustrated persistence of luminescent signal, indicating engraftment of human cells, in hearts that received hESC-EC injections and coinjections of hESC-ECs and hESC-SMCs (Fig. 2C). Measurable signal from engrafted cells in the left ventricle was detected throughout the course of the 4-week study and in excised hearts (Fig. 2E). Some animals receiving both hESC-ECs and hESC-SMCs were assessed for luciferase signal beyond the 4-week study. Remarkably, the luminescent signal actually increases at later time points (8 weeks), demonstrating stable engraftment and expansion of these cells (Fig. 2D). Additional immunohistochemical studies demonstrated development of GFP+ vascular cells at 4 weeks post-transplant, again demonstrating survival and function of these hESC-derived cells in hearts with postinfarction LV remodeling (Fig. 2F).
Because of the significant difference in heart rate between mouse and human (600 beats compared with 70 beats per minute), and the arrhythmia concerns that associated with the human cells engraftment in the mouse heart, we combined these novel cell types derived from hESC in a 3D porous biodegradable fibrin patch to a clinical relevant pig model of ischemia-reperfusion and postinfarction LV remodeling. To assess the therapeutic potential of the cellularized patch, we examined LV chamber and regional myocardial contractile function, LV wall thickness and systolic wall stresses, scar size, and chamber function.
Swine Studies
Anatomic Data
Cell transplantation resulted in a significant decrease in LV hypertrophy as reflected by a decrease in LV weight/body weight (LVW/BW) (Fig. 3A; p < .05) in the cell treated hearts as compared with the hearts in both of the control groups. The myocardial infarct size data measured by MRI are illustrated in Figure 3B. At week 1 post LAD ligation, the infarct size was about 9% of total LV, and was not significantly different among the three groups (p = NS). However, at week 4, the infarct size was significantly smaller in cell-treated group than the MI group (p < .05).
LV Contractile Functional Data
The temporal changes in EF as measured by MRI are illustrated in Figure 4A. There was no significant difference among the three groups at baseline. However, the fibrin patch-based cell transplantation showed a significant improvement in EF, which was seen as early as 7 days (MI+P+C [45.2 ± 3.1] vs. MI [39.2 ± 3.0], p < .05). The beneficial effect in LV chamber function persisted up to 4 weeks (Fig. 4A; p < .05). Similarly, the regional LV systolic thickening fraction in the infarct zone was also significantly improved in cell-treated group compared with the other two control groups (Fig. 4B; p < .05). In addition, the cell treatment resulted in reduction of the LV thinning in the infarct zone seen at 4 weeks after MI, as depicted by significantly lower across the LV wall thickness in the saline treated- or open patch treated-groups (Fig. 5A; p < .05). This increased LV wall thickness in the infarct zone was accompanied by a significant lower systolic LV wall stresses as compared with MI control group (Fig. 5B; p < .05).
Vascular Density
To determine the mechanisms underlying the beneficial effects of cell transplantation, we investigated the effects of cell transplantation on neovascularization and angiogenesis in the post-MI hearts. At 4 weeks after cell transplantation, immunofluorescence staining for von Willebrandfactor (vWF) antibody indicated significant angiogenesis in stem cell-treated hearts, with more vWF-expressing capillaries being present in both infarct and peri-infarct regions of cell treated compared with the other two groups (Fig. 6A). Quantitative evaluation of vWF-positive capillary fractional area per high-power field (×20) indicated that vascular density was significantly greater in the cell-treated group than the control groups of saline-treated and open patch-treated hearts (Fig. 6B). In the peri-infarct region of cell-transplanted hearts, double vWF+/GFP+ cells were observed via costaining of vWF and GFP, however, few cells were stained positive for both the myocyte marker TnT or MHC and GFP (data not shown). These data suggested that transplanted hESC-derived cells may rescue ischemia threatened myocytes from apoptosis, promote angiogenesis through a paracrine effect.
Cell Engraftment
There was significant engraftment of hESC-derived endothelial cells and smooth muscle cells observed at 4 weeks after transplantation in the cell-treated animals of both mouse (4 weeks, Fig. 2C–2E) and swine (day 9, 16 and 27, Fig. 7) model of postinfarction LV remodeling as confirmed by in vivo and ex vivo BLI.
- Top of page
- Abstract
- Acknowledgements
- Supporting Information
This study demonstrates that a fibrin patch-based hESC-derived vascular cell transplantation leads to functional improvement in a clinically relevant porcine model of postinfarction LV remodeling. Stem cell transplantation resulted in an overall improved LV function and LV remodeling following MI with an amelioration of LV scar thinning. This wasassociated with a reduction in scar size at 4 weeks. Cell transplantation also resulted in a significant increase of neovascularization in both infarct and peri-infarct regions. These data demonstrate that using a novel 3D porous fibrin patch-enhanced delivery, the transplantation of the equal mixture of endothelial cell and smooth muscle cells derived from the hESCs can promote the myocardial neovascularization of the recipient heart, reduce the ischemic injured myocytes from apoptosis, and improve the LV contractile function. The findings suggest a promising therapeutic potential of using this cell type and mode of delivery.
Translational Potential of hESC-Derived Endothelial and Smooth Muscle Cells
hESCs have been used to derive diverse cardiovascular cell populations [24]. Reverse transcriptase polymerase chain reaction and immunohistochemical studies demonstrate that hESC-derived cardiomyocytes (hESC-CMs) express early cardiac-specific transcription factors, sarcomeric proteins, and gap junction proteins [19]. Although, electrophysiologic studies showed that most of the hESC-CMs resemble human fetal ventricular myocytes capable of propagating action potentials [25], a recent study suggested that transplantation of hESC-CMs with serum-free medium to hearts with injuries resulted in a very low engraftment rate [19]. On the other hand, there are reports demonstrating that beneficial effects of LV chamber function in response to the bone marrow-derived progenitors transplantation therapy, is associated with the increased neovascularization [7, 9, 10, 12]. Consequently, the present study was carried out to examine whether hESC-derived cardiac vascular cells can be used to prevent LV dysfunction and postinfarction LV remodeling in hearts suffering from acute myocardial infarction. The data from the present study demonstrate that these cell types can promote the myocardial neovascularization of the recipient heart (Fig. 6), which in turn, reduce the ischemic-injured myocyte from apoptosis and improve the LV chamber function.
Engraftment of hESC-Derived Cells
Using the novel fibrin patch-enhanced delivery, data from both the mouse model and pig model demonstrated significant cell engraftment a few weeks after the transplantation, which was accompanied by the functional and bioenergetic improvement. Recently, there are reports suggesting that less than 50% of the cells remained in the myocardium a few hours after the transplantation, which was caused by the pulsate intramyocardial pressure changes during the continuous cardiac cycle [26]. These data suggest that a significant fraction of the acute loss of delivered cells is caused by the active contraction of the LV but is independent from the apoptosis or necrosis.
In this study, the BLI data from both rodent and swine studies demonstrate the persistence of hESC-derived cells (Figs. 2 and 7), which is in agreement with our earlier reports that applying this 3D porous biomaterials in stem cell delivery for myocardial repair is accompanied by further increase of cell engraftment rate, reduction in apoptosis in ischemia-threatened myocytes, decrease of peri-infarct area fibrosis, and prevention of LV scar systolic bulging [7]. The reduced LV dilatation and LV scar bulging, in turn, result in significantly reduced myocardial wall stress and improved myocardial bioenergetics.
This fibrin 3D porous biomatrix is useful for several reasons. First, it is tightly patched on the surface of injured myocardium that had exposed to ischemia reperfusion. Therefore, the active myocardial contraction will not squeeze the cells out of the site of the delivery. Second, there are ample fibrinogen and thrombin proteins in the circulation that can be used for the patch biomaterial. In principle, both components can be autologous. In this study, we try to use the autologous thrombin by scraping the surface of myocardial patch area, which effectively make the patch tightly stick on the surface at 4 weeks post transplantation. The scraping channels may also serve as routes for the stem cells get into the myocardium. Third, the fibrin patch can be chemically modified to bind any peptides such as hepatocyte growth factor or SDF-1α, creating a progenitor cell friendly environment for the purposes such as enhancing the engraftment or controlled differentiation [7, 14–16]. Fourth, we have previously demonstrated that the MSC fibrin patch itself result in a remarkable increase of neovascularization into the patch material 4 weeks after the transplantation [7, 14–16], which was accompanied by a significant increase of engraftment rate and improvement of systolic thickening fraction in the patched area of myocardium. In the present study, we have found that this patch delivery is associated with significant engraftment rate (Fig. 7). In some cases, the BLI signals indeed increased. Most importantly, the data from the present study demonstrate that both chamber function and regional systolic thickening fraction improved significantly (Fig. 4).
Improvement in Ventricular Function and Reduction in Regional Wall Stress
This study demonstrates that transplantation of hESC-derived vascular cells leads to improvement in ventricular function in a large animal model. We have previously reported that LV bulging at infarct zone was accompanied by a significant increase of regional LV wall stress at IZ and peri-infarct BZ [2]. This particular increase of regional wall stresses and its associated severe bioenergetic abnormality were significantly ameliorated by the autologous MSC transplantation [7, 11, 12]. We reasoned that the decrease of LV bulging at LV scar area (Fig. 4B) resulted in reduction of the regional wall stress (Fig. 5B) and energy demand, which in turn, would otherwise cause severe bioenergetic abnormality [27]. In the present study, the engraftment of the hESC-derived vascular cells (Fig. 7) was accompanied by increase of LV wall thickness and thickening fraction in the IZ and BZ, which in turn, resulted in a significant reduction of regional LV wall stresses (Fig. 5B). By necessity, these will result in the improvement of myocardial bioenergetics that has been observed using the same pig model of postinfarction LV remodeling [12].
During the 4 weeks follow-up by cardiac MRI, we observed a significant reduction of infarct size (Fig. 3B) that occurred only in the MI+P+C group. The LV dysfunction of failing hearts is associated with the oxidative stress caused by the excess of reactive oxygen species production in the cytoplasm and the electron transport chain of mitochondria in myocytes [28]. Using a similar animal model and bone marrow-derived progenitor cells, we also observed that cell transplantation-associated improvement in myocardial contractile function was accompanied by reduction of several different subunits of the respiratory chain oxidative enzyme, which may in turn, result in the reduction of the oxidative stress, and consequently reduce the infarct size associated with apoptosis [11].
Effect of Immunosuppression
In the current pig study, cyclosporine A was administered for immunosuppression. This approach has been utilized previously in a similar pig study with bone marrow-derived multipotent progenitor cell transplantation [12]. In that study, there was no significant difference in the LV postinfarction remodeling between the cell-treated hearts with and without cyclosporine A administration. From the same study, pigs (3 months of age) that received myocardial infarction and no cyclosporine A had an average body weight of 29 ± 7 kg and a mean LVW/BW ratio of 3.57 ± 0.27. In the current study, pigs that received similar myocardial infarction and cyclosporine A had an average body weight of 35 ± 3 kg at age 3.4 months and mean LVW/BW of 3.6 ± 0.3. These data indicated that in pigs, the cyclosporine A does not have an obvious influence on the LV remodeling after myocardial infarction.
Effect of Cell Transplantation on Infarct Size
Sequential MRI functional assessment allowed us to conclude that the infarct size in swine treated with hESCs decreased from 1 week to 4 weeks interval, whereas the scar sizes in swine without hESC treatment was maintained throughout the follow-up (Fig. 3B). This reduction in scar size with stem cell treatment has been seen previously in other reports [6, 29, 30]. The beneficial effects of cell transplantation could be related to the mobilization of endogenous cardiac progenitors to the injury site by paracrine effects as we and others have observed previously [6, 30]. As we did not observe significant myocytes transdifferentiation at 4 weeks, the functional and structural improvements of decrease of infarct size and improved vascular density, are likely secondary to paracrine effects that include sparing of native cardiomyocytes from apoptosis as we observed recently [31]. It was also observed recently that cell transplantation is accompanied by an upregulation of myocyte enhancer factor-2 A (MEF2A), the predominant MEF2 gene product expressed in postnatal cardiac muscle. MEFs play an important role in myogenesis [32]. We postulate that cell transplantation may cause an activation of endogenous cardiac progenitor cells [6, 33], and MEF2A may play a role in their differentiation into cardiomyocytes.
- Top of page
- Abstract
- Acknowledgements
- Supporting Information
In summary, this study demonstrates that a fibrin patch-based transplantation of hESC-derived vascular cells (ECs and SMCs) leads to a significant engraftment of vascular cells derived from the hESCs that is accompanied by a significant increase of neovascularization and LV contractile functional improvement, which in turn, results in a significant reduction of regional wall stress and infarct size. Taken together, these findings suggest a promising therapeutic potential of this combined approach using these vascular cell populations derived from hESC and the novel mode of delivery.
- Top of page
- Abstract
- Acknowledgements
- Supporting Information
This work was supported by U.S. Public Health Service Grants HL50470, HL67828, HL 95077, HL100407, and HL077923 and the Engdahl Family Foundation.
- Top of page
- Abstract
- Acknowledgements
- Supporting Information
The authors indicate no potential conflicts of interest.
- Top of page
- Abstract
- Acknowledgements
- Supporting Information
- 1Left ventricular remodeling after primary coronary angioplasty: Patterns of left ventricular dilation and long-term prognostic implications. Circulation 2002; 106: 2351–2357., , et al.
- 2Profound bioenergetic abnormalities in peri-infarct myocardial regions. Am J Physiol Heart Circ Physiol 2006; 291: H648–H657., , et al.
- 3Transcoronary transplantation of progenitor cells after myocardial infarction. N Engl J Med 2006; 355: 1222–1232., , et al.
- 4Transplantation of progenitor cells and regeneration enhancement in acute myocardial infarction (TOPCARE-AMI). Circulation 2002; 106: 3009–3017., , et al.
- 5Bone marrow cells regenerate infarcted myocardium. Nature 2001; 410: 701–705., , et al.
- 6The Role of Sca-1+/CD31- cardiac progenitor cell population in postinfarction left ventricular remodeling. Stem Cells 2006; 24: 1779–1788., , et al.
- 7Autologous stem cell transplantation for myocardial repair. Am J Physiol Heart Circ Physiol 2004; 287: H501–H511., , et al.
- 8Xenotransplantation of long term cultured swine bone marrow-derived mesenchymal stem cells. Stem Cells 2006; 25: 612–620., , et al.
- 9Bioenergetic and functional consequences of stem cell-based VEGF delivery in pressure-overloaded swine hearts. Am J Physiol Heart Circ Physiol 2006; 290: H1393–H1405., , et al.
- 10The role of the sca-1+/CD31- cardiac progenitor cell population in postinfarction left ventricular remodeling. Stem Cells 2006; 24: 1779–1788., , et al.
- 11Stem cells for myocardial repair with use of a transarterial catheter. Circulation 2009; 120: S238–S246., , et al.
- 12Bioenergetic and functional consequences of bone marrow-derived multipotent progenitor cell transplantation in hearts with postinfarction left ventricular remodeling. Circulation 2007; 115: 1866–1875., , et al.
- 13Allogeneic mesenchymal stem cell transplantation in postinfarcted rat myocardium: Short- and long-term effects. Circulation 2005; 112: 214–223., , et al.
- 14Enhancing efficacy of stem cell transplantation to the heart with a PEGylated fibrin biomatrix. Tissue Eng Part A 2008; 14: 1025–1036., , et al.
- 15A PEGylated fibrin patch for mesenchymal stem cell delivery. Tissue Eng 2006; 12: 9–19., , et al.
- 16Controlled release of stromal cell-derived factor-1 alpha in situ increases c-kit+ cell homing to the infarcted heart. Tissue Eng 2007; 13: 2063–2071., , et al.
- 17Human embryonic stem cell-derived vascular progenitor cells capable of endothelial and smooth muscle cell function. Exp Hematol 2010; 38: 246–257., , et al.
- 18Efficient and stable transgene expression in human embryonic stem cells using transposon-mediated gene transfer. Stem Cells 2007; 25: 2919–2927., , et al.
- 19Cardiomyocytes derived from human embryonic stem cells in pro-survival factors enhance function of infarcted rat hearts. Nat Biotechnol 2007; 25: 1015–1024., , et al.
- 20The host immune response is essential for the beneficial effect of adult stem cells after myocardial ischemia. Exp Hematol 2007; 35: 682–690., , et al.
- 21Functional and bioenergetic consequences of postinfarction left ventricular remodeling in a new porcine model. MRI and 31 P-MRS Study. Circulation 1996; 94: 1089–1100., , et al.
- 22Correction of logistic regression relative risk estimates and confidence intervals for systematic within-person measurement error. Stat Med 1989; 8: 1051–1069; discussion 1071–1073., , .
- 23Wnt signaling promotes hematoendothelial cell development from human embryonic stem cells. Blood 2008; 111: 122–131., , et al.
- 24Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiomyocytes. J Clin Invest 2001; 108: 407–414., , et al.
- 25Electromechanical integration of cardiomyocytes derived from human embryonic stem cells. Nat Biotechnol 2004; 22: 1282–1289., , et al.
- 26Death and proliferation time course of stem cells transplanted in the myocardium. Mol Imaging Biol 2009; 11: 408–414., , et al.
- 27Relationships between regional myocardial wall stress and bioenergetics in hearts with left ventricular hypertrophy. Am J Physiol Heart Circ Physiol 2008; 294: H2313–H2321., , et al.
- 28
- 29Cardiac repair with intramyocardialinjection of allogeneic mesenchymal stem cells after myocardial infarction. Proc Natl Acad Sci USA 2005; 102: 11474–11479., , et al.
- 30Evidence supporting paracrine hypothesis for Akt-modified mesenchymal stem cell-mediated cardiac protection and functional improvement. FASEB J 2006; 20: 661–669., , et al.
- 31Long term functional improvement and gene expression changes after bone marrow derived multipotent progenitor cell transplantation in myocardial infarction. Am J Physiol Heart Circ Physiol 2010; 298: H1348–H1356., , et al.
- 32Transcriptional control of muscle development by myocyte enhancer factor-2 (MEF2) proteins. Annu Rev Cell Dev Biol 1998; 14: 167–196., .
- 33Intracoronary administration of cardiac progenitor cells alleviates left ventricular dysfunction inrats with a 30-day-old infarction. Circulation 2010; 121: 293–305., , et al.
Supporting Information
- Top of page
- Abstract
- Acknowledgements
- Supporting Information
Additional supporting information available online.
|Supporting Information
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.


text:
- Police/Fire/Courts
- Business
- Local
- State/Northeast
- Nation/World
- 2016 Elections
- Special Reports
- Maps & Data
- Military
- Horoscopes
- Dear Abby
- Games & Puzzles
- Lifestyle
- Events & Exhibits
- Food & Drink
- Arts & Music
- Movies & TV
Norwich — U.S. Sen.-elect Chris Murphy said Tuesday that he will work to exclude education, transportation and science funding from any federal budget cuts next year because he believes they are critical to reviving Connecticut's lagging economy.
"These investments matter more here," Murphy said in delivering a keynote speech at a breakfast meeting of the Chamber of Commerce of Eastern Connecticut before about 150 people at the Holiday Inn.
Murphy praised Gov. Dannel P. Malloy for increasing investments in the biosciences, primarily in the Farmington area, saying that he believes the benefits eventually will spill over to eastern Connecticut, despite the "agitas" of some economic leaders in this region who believe more should be done to revive fortunes here as Pfizer Inc. reduces its presence in Groton. He said these "overdue investments" can only help Connecticut's economy, which has failed to create any job growth over a two-decade period.
"We have generally been lacking a cohesive economic strategy in this state for about 20 years," Murphy said.
Murphy said his policy agenda includes a push to see that the Central Corridor rail initiative linking southeastern Connecticut with Canada and points north moves forward.
"I think it is economic genius to recognize the importance of commuter rail," he said.
But while Murphy favors holding the line on some federal budget cuts, he acknowledged the need to reduce defense spending even as he committed to protecting the Navy's projected two-submarines-a-year production schedule that benefits Electric Boat.
Murphy, saying "we're in the middle of a global recession," acknowledged the need to reduce the federal debt by $3 trillion to put the country on track to "leapfrog the rest of the world in terms of the economy." But Murphy said that, instead of across-the-board cuts, he would support targeted reductions that leave key economic drivers and investments in place.
"I see enormous economic rewards ahead if we do it right," he said.
In addition, Murphy said he could see benefits from lowering corporate tax rates and simplifying the federal tax code, which might include reducing itemized deductions.
But Murphy said any reductions should be accompanied by policy changes that would ease the impact of the cuts. Among his ideas is to lessen dependence on foreign contractors by instituting a more aggressive buy-American campaign on the federal level.
"As we shrink the pie ... we have to increase the slice spent on American companies," he said.
Murphy, who has been representing northwestern Connecticut as the 5th District U.S. congressman, introduced himself to business leaders as a die-hard Red Sox fan who has roots in the region. His great-grandfather, the ninth of 13 children, was born in Norwich, he said, and became a probate judge in the Windham area while also heading a construction company that built dozens of structures, primarily in eastern Connecticut.
"I feel like I've come home, in a way," said Murphy, who lives in Cheshire.
Murphy promised to take a pragmatic approach to public-policy issues and to reach across the aisle to form relationships that will address key concerns, including immigration reform. He added that reducing business regulations will be part of his agenda in Washington.
"We can be a more business-friendly state," he said. "That's just a choice. It's not part of the genetic code of the state."
Lyme man killed in East Haddam crash was to begin serving 10-year prison sentence
Norwich man charged with fraudulently collecting unemployment compensation
UPDATED: Foxwoods names architect for Biloxi, Miss., casino project
Appellate Court rules against Waterford commission in Kobyluck Bros. rock-crushing case
Bill O'Reilly: Slaves that built White House were 'well-fed'
East Lyme resident to host community discussion on race on Friday
Grant would update guide rails, signage on Ledyard road with high number of accidents
Casey & The Sports Doctor: Caroline Taber, rules changes, Matt Harvey
Waterford fabricator, national barbecue champion team up to design and manufacture pits


text: Customize Your Own Super Mini Skateboard, 7" x 25" Complete. Your Super Mini Complete Skateboard has options for your grip tape, bottom color, truck color, and wheel color. All decks have a natural wood color top and include a screen-printed logo on the bottom of the board in the high right position. The Skateboard Mini is extremely portable and lightweight. The Mini Complete Skateboard is perfect for cruising school campuses or skating to a friend's house.


text:
Italian Premier, ECB Chief Support Bigger ESM, Spiegel Reports
Italian Prime Minister Mario Monti and European Central Bank President Mario Draghi both support enlarging the capacity of Europe's permanent financial rescue mechanism, Der Spiegel reported, without saying where it got the information.
The news magazine said Monti is pushing for the European Stability Mechanism's capacity to be doubled to 1 trillion euros ($1.29 trillion), and had made the suggestion to the German government. Der Spiegel added that Draghi supports the view that unused funds from Europe's temporary rescue fund should be added to the ESM's firepower when it comes into force.
Before it's here, it's on the Bloomberg Terminal. LEARN MORE


text:
Rachel-erika Henderson
(New Zealander Writer)
- Date of Birth:
- December 16, 1978
- Nationality:
- New Zealander
- Quotes Submitted:
- 80
- Times Favorited:
- 48
Born in New Zealand in 1978 she had a love for poetry and lyrics writing from a young age. In her teens she set off to travel the world. She has five published books. She currently resides on the Isle of Skye with her daughter Daisy and still writes daily.
Rachel-erika Henderson's site…
Rachel-erika Henderson's favorite quotes
sorted by Date, Tag, Author A-Z, Submitted Quotes
- 60's
- Beauty
- Confusion
- Crazy
- Dance
- Day
- Death
- Dimension
- Dream
- Dreams
- Embrace
- Everything
- Excuse
- Free-thinking
- Friendship
- Guidance
- Happiness
- Imperfections
- Intelligence
- Judge
- Judgement
- Life
- Love
- Lyrics
- Me
- Media
- Memory
- Music
- Nature
- Open-minded
- Orgasm
- Party
- Perfect
- Philosophy
- Poetry
- Quote
- Solve Problems
- Someone
- Song
- Spirit
- Spiritual
- Step
- Summer
- Sun
- Sunshine
- Thoughts
- Wisdom
- Woodstock
- Writing
- Youth
"I am buried alive in Love A tremendous hurtful desire There is dirt on my face The hills are filled with fire If my life were all alone Not one soul did I greet My own soul and heart Would never ever meet But this is a burden This love I carry inside It brings hurt along with love I take this in my stride When we are seperated in life Will we strike an eternal bond This love shared in our lifetime Will be continued far beyond"
"I am almost 126 seasons old I've been through warmth and cold Each season casts a different array They brought me here to you today Each flower that marks the start of spring The summer sun, the songs we sing then autumn with the leaves that fall finally winter, the coldest of all My life has changed along the way but the best thing is being with you today"
"The unborn living and the living dead escape from your mind open another dimension in your head Don't just exist start to believe A tree is not always just a tree look beyond the vision you have Look for what you can't always see"
"Sometimes I feel a little thick then I realise, I just have a bad memory! None of my friends are scientists they have all been told these things that stick in their memory (yet seem foreign to me) I on the other hand have a crap memory and cannot remember alot!"
"Where the clouds reach down to kiss the earth, This is the place of my rebirth. In my numbered days I need time to rest For in my physical body I remain a guest"
"I'm just sitting with a dram waiting on someone creating a time machine so I can get a lift back to the 60's"


text:
This page requires a MaxPreps membership account.
Don't stress... It's free and easy to setup.
The Archbishop Mitty Monarchs basketball team won Saturday's neutral non-conference game against Oakland by a score of 54-37.
(December 3, 2011: San Jose, CA 95129) The Archbishop Mitty Monarchs topped the visiting Oakland Wildcats basketball team by a final score of 54-37 in Saturday's non-league game.
In their next game, the Monarchs face the Palma Chieftains (Salinas, CA) in a non-league game, on Tuesday, December 6. Archbishop Mitty will attempt to move on its 1-0 season record. The Chieftains enter the game with a 1-0 record after their 55-38 non-league win over Carmel (Carmel, CA).
With the defeat, Oakland moves to 0-2 on the year. The Wildcats travel to Natomas (Sacramento, CA) to face the Nighthawks in a non-league game on Wednesday, December 7. The Nighthawks enter the game with a record of 1-2. Natomas lost 68-62 in their recent non-league game against Granada (Livermore, CA)


text:
PSA: Xbox Live Gold is free this weekend
This is just a friendly reminder to folks out there, that Silver members of Xbox Live can play online free this weekend.
Microsoft announced back in August this free weekend was coming, but it's been a while, and you may have forgotten about it. We did at any rate.
This means, that until tomorrow, you can play Halo: Reach online with your Gold buddies. Or whatever else floats your boat.
Get more information about it through Xbox.com.
Thanks, SavvyD.
Sometimes we include links to online retail stores. If you click on one and make a purchase we may receive a small commission. For more information, go here.


text:
Rockit Sounds R-50
"Just want to state up front my review rating is an absolute rating, not one based on comparative performance, pricing etc. These are my observations after using an iBasso DX50, MacBook air, FiiO E17 and Sansa Clip Zip as test sources. I found very little variation between those sources with the DX50 maybe bringing out a little bit more richness and depth. Highs: Well what can I..." --Krisman
Rockit Sounds R-Studio
"full review can be found here: http://www.head-fi.org/t/685909/review-rock-it-sounds-r-studio-2nd-strike Build & Comfort: The R-Studio take a Circum-Aural design, with a "neo-classic" look as Rock-It call them. They do have some "retro" air on them along with the usual company color scheme. But not everything is looks, right? Not a huge step as it's still made mostly of..." --Zelda
Astro A50 Wireless Headset
"I've owned this headset for about 1 year now I purchased it through this twitter account I follow called IGN deals. Got it for $200. I play regularly everyday for 4+ hours. And I see why there are no reviews for this headset considering it is one of the best and thats just what it's known for. It has a switch that gives you 3 different eq settings. The wireless receiver gives you the option for..." --MillzBaby
ASTRO Gaming A30 Wireless System (Black)
" The sound quality is a lot better than they make it seem, the marketing shows it being used with PSPs and phones, but they are capable of handling more than angry birds. I use these for PC gaming and music. The sound on these almost compare to my Sony MDR EX700LPs, but with a better soundstage and less bass extension. I actually prefer these to the A40s, they..." --jimmy895
Astro A40 Audio System - White
"Ok first I need to say that I'm not an audiophile, and I use this site mostly for help than giving advise whit that told I will continue I've owned this headset for more than a year and they are really good, if you're looking for a headset that can be used with the xbox, xbox one , ps3, ps4, and not only in your pc and ipod, mp3 player and so but it have an average sound ,..." --MaxiMarciano
Rockit Sounds R-DJ
"The R-DJ is part of Rock-It's new lineup of headphones, complete with the signature Rock-It styling excellence. Accessories - Straight cable, coiled cable Simplicity aside, these cables are, without a doubt, the sturdiest and thickest detachables that I've ever felt. Not to mention, they literally "fit" perfectly with the headphone's design. Build/Design - As..." --pro1137
A40 TR Headset + MixAmp Pro TR for PS4
Redefining pro, the new ASTRO A40 TR headset and Mix Amp features user customization to adapt to any environment. Ready for home use, LAN play or the loudest pro tournament, you can transform the A40 TR from an open-back to a closed-back noise cancelling headset by swapping components like speaker tags, microphone and ear cushions (sold separately in ASTRO Mod Kits). For serious live streamers,...
Astro Gaming A*Star In-Ear Headset
Cut through the noise. The bold design of the ASTRO A*STAR™ In-Ear Headset is the ultimate in hack-n-slash combat; featuring an in-line medallion with mic, volume dial and answer/hang up button, flat tangle-resistant cables, a PC splitter, three sizes of ear gels and a handy carrycase. Inspired by the light-weight materials and aggressive forms of high performance headgear, the A*STAR...
Rockit Sounds R-30
In-Ear-Monitor earbuds Designed for the professional user who want the best Tubular Balanced Armature speakers for high performance acoustics Non-allergic & flexible silicone INNER & OUTER ear gels keep the noise out Professional grade customized fitting kit with 10 fitting options Innovative & fun 'twisty liquorice' cable to...
Rockit Sounds R-11
Rock It Sounds R-11 Item# R-11


text:
Track using Order ID
Login and do more!
- Track individual Orders
- View your entire Order history
- Cancel individual Orders
- Conveniently review products and sellers
Unique Formulation
Shahnaz Husain brings you the goodness of turmeric, lime, saffron and honey to ensure you receive all tested traditional ingredients but along with these the soap is formulated with a unique formulation consisting ayurvedic ingredients, which are rich in oils and nutrients that your skin needs.
Gentle Dermabrasive Action
The soap gives your skin deep pore cleansing that removes impurities deep seated within the layers and pores of your skin by presenting an efficient scrubbing effect that acts as a dermabrasion of your skin. The soap will remove dead skin, allergens and bacteria and any other unwanted elements to leave behind healthy skin.
Enriched With Moisturizers
As the formulation is ayurvedic, the soap is enriched with various oils that will supply your skin with enough moisture to keep your skin feeling soft and not dry or rough. Instead of only removing, the soap also gives back to your skin the support to replenish moisture.
Gives Soft, Radiant and Youthful Skin
With the nourishing effects of its ingredients, the soap leaves your skin softer and clearer so you flaunt gorgeous skin that is enriched with nature's essence to regain a youthful look.
Brighter and Visibly Fairer
As it is a bar that improves your skin's fairness, after it is done with cleansing the soap nourishes your skin with the gentle bleaching agents of lime and whitening effects of saffron to give you visible fairer skin.
|Soap Essentials
|Organic Type
|Shahnaz Husain
|Skin Type
|All Skin Types
|Soap Application Area
|Soap Applied For
|Deep Pore Cleansing, Gentle Dermabrasive Action
|Soap Type
|Beauty Soap
|General Traits
|100 g
|Other Traits
|Gives Scrubbing Effect, Enriched with Moisturizers, Soft, Radiant and Youthful Skin
|Model Name
|Shafair Ayurvedic Fairness Soap
|Ideal For
|Container Type
|Nariyal Tel, Arandi Tel, Yashad Bhasam, Kheea Beej Extract, Arishtak Extract, Gulab Ark, Nimbu Patra Extract, Honey, Haldi Extract, Kesar Extract, Abhrak
Please Note: All products sold on Flipkart are brand new and 100% genuine
Have you used this product?
Rate it now.Write a Review
'Top Reviews' lists the most relevant product reviews only. Show ALL instead?Expand all Reviews
Vinoth Kumar
Sep 16, 2015
Purchased for my colleaguePurchased for my colleague he said it was most expensive and will hold only for 2 weeks for only the face wash
Flipkart Customer
Apr 27, 2014
I liked this nice product.when i washed my face with this soap it made my skin smooth clean. there was no soapy effect. there was no irritation on skin after using this soap.little dries the skin. i used a moisturizer .
Ramesh Paladi
Mar 13, 2014
Good SoapGood Soap from Shahnaz Husain, bit costly. Looking for best deal. Thank again to Flipkart for great packing and fast delivery.
Safallya Pal
Feb 17, 2014
a good scrubberthis soap fails to fulfill the fairness part as it claims with regular use it will give fairer skin... but it doesn't do so.. but otherwise this soap is quite good, fragrance is awesome.. scrubbing effect is also too good! but another negative point it finishes off very soon... anyway, that's what i felt, other than that , its worth to give a try..
Bineet Anand
Jan 25, 2014
BEST SOAP EVER!!!Have been using it for a while now, though expensive, still worth buying.
Feb 22, 2013
good soap but costlyi hav tried this soap its scrub feeling is awesome and its not only for women but men can also use it.it is made up of natural herbs so it is safe to use. it leaves skin soft and smooth but little dry. overall it is good soap.


text:
Kingdom Hearts Re:Coded gets new shots
Square's quietly sneaked out half a dozen new shots of Kingdom Hearts: Re:Coded.
The title, a DS version of Coded for Japanese, sees new screens of stages from Alice in Wonderland, Hercules and Aladdin.
There's more on RPG Site.
Kingdom Hearts: Re:Coded releases on Tuesday in the US and next Friday in the UK for DS.
Sometimes we include links to online retail stores. If you click on one and make a purchase we may receive a small commission. For more information, go here.


text:
"The Land of the Rising Sun" Top 5 Page for this destination Mpumalanga by cokes
Mpumalanga Travel Guide: 74 reviews and 255 photos
Mpumalanga, the place of the rising sun, is one of South Africa's newest and fastest growing provinces.
Created immediately after the country's first democratic elections in 1994, Mpumalanga covers 6,5% of South Africa's surface area, constituting an area larger than Belgium and the Netherlands combined.
Its approximately three million people generate R55 billion, or 8%, of the country's annual economic output and enjoy a higher than average quality of life and faster economic growth rate than any other province.
This is a place with a real view of Africa , Huge Mountains covered in Forest and Home to the Big 5 "Kruger National Park"
- Pros:Lovely Place to Get Away , Best for Safari
- Cons:Some Places Here is Malaria Infected Areas
- In a nutshell:Wild and Untouched
Reviews (15)
My stay here was nice. The rooms is quiet spacious and nice. There is a spa here which is quiet nice. Also there is a... more travel advice
This is also another nice waterfall. When following the path to the waterfall please be aware that the path leads to the... more travel advice
The Berlin Falls is just down the road from the Lisbon Falls. Entrance here is also R5 per person. This waterfall is not... more travel advice
This is my opinion one of the most beautiful waterfall in the area and also the highest. It`s situated just outside... more travel advice
Been to Mpumalanga?Share your travels with the world!
Comments (11)
Hadn't heard of this province. What a lovely place it is. I especially like your waterfall photos!
hello featured VT member. this is a great page on slightly less known of south african destinations. i liked your pictures and writing style.
Early morning in Sabie = Great photo!
Ha ha...can you imagine anyone driving on those? they look like somewhere in outerspace;p Talking about malaria, is it still a big issue over there? I remember when I was a little girl in Nigeria, I had to get those awful shots.
Thanks for your reply about the shoe size Collin. Very nice page here. Beautiful photos. I'll be in your neck of the woods soon! Cheers :)
Very good page here. Wow at the leopard picture - did you take it yourself?
Gorgeous photos, looks like a really interesting place
Never thought forest can look so nice. I'm impress. Great photography.
I got drawn to this page by your stunning Sabia photo on the homepage. South Africa looks so beautiful, Great little page, thanks (~_~)
Amazing photos! That's definitely a place I'd love to visit!
cokes' Related Pages
Mpumalanga Travel Guide
Member Travel Pages
- "The Land of the Rising Sun"
- "Graskop and the Blyde River"
- "The natural beauty of Mpumalanga"
- "Mpumalanga"
- "Mpumalanga - It means 'sunrise' you know"
- "Game for some wildlife adventure?"
- See All...
- Things to Do in Mpumalanga
- Hotels in Mpumalanga
- Transportation in Mpumalanga
- Restaurants in Mpumalanga
- Shopping in Mpumalanga
- Warnings and Dangers in Mpumalanga
- See All...
"Go out there without expectations , just go and see for yourself"
- Member Rank:
- 0 0 3 1 5
- Forum Rank:
- 0 1 8 8 4
- 583 Reviews
- 1,952 Photos
- Add Friend
- Follow
- Send Message
Badges & Stats in Mpumalanga
- 15 Reviews
- 48 Photos
- 0 Forum posts
- 12,735PageViews
- 0 Cities
- See All Stats
- See All Badges (14)
Have you been to Mpumalanga?Share Your Travels
Latest Activity in Mpumalanga
- updated a Mpumalanga Travel Page "The Land of the Rising Sun"
- Posted in Travel Bali Forum "Re: Value for money location in..."
- Uploaded a Photo to "Spa Lodge in Sabie"
- Wrote a Review Spa Lodge in Sabie in Mpumalanga Hotels
- Commented on one of Jenniflower's Mpumalanga travel pages
Photos in MpumalangaSee All Photos (48)
Top 10 Pages
- Top 5 Page for this destination Johannesburg Intro, 118 reviews, 265 photos, 7 travelogues
- Top 5 Page for this destination Cape Town Intro, 75 reviews, 227 photos, 6 travelogues
- Top 5 Page for this destination Sun City Intro, 67 reviews, 216 photos, 7 travelogues
- Jakarta Intro, 28 reviews, 128 photos, 7 travelogues
- Top 5 Page for this destination Hartbeespoort Intro, 42 reviews, 109 photos, 5 travelogues
- Top 5 Page for this destination Bali Intro, 35 reviews, 99 photos, 4 travelogues
- Singapore Intro, 25 reviews, 99 photos, 3 travelogues
- Yogyakarta Intro, 12 reviews, 110 photos, 6 travelogues
- Kuala Lumpur Intro, 21 reviews, 88 photos, 3 travelogues
- Top 5 Page for this destination Durban Intro, 21 reviews, 78 photos, 1 travelogue


text:
Type: Posts; User: rockn_yota
Miller matic 140XP
I see MC run through SEALTITE all the time up here, can you remark this? being its a conductor through a raceway
Man it sure is nice to have equipment manu. lurking around on here to help!! I think you need 6 outlets/ton, your suction pressure is going to run %15 lower like MIK said
city code wont let us use clear cleaner:( we have had this discussion at the shop multiple times.
this is the first system like this we have done, so their was a huge learning curve for all of us. The water lines were done by another company but we are responsible for all the rest of the flaws...
i have a system with a amvm and a 18 seer communicating heat pump with a EWC communicating zone panel, in heating i have found that the heat pump's timing of turning the blower on in the furnace can...
I didn't do the linesets those will be fixed first of next week and tiddy up the wiring.
2 systems
tts038 climate master tranquility 27 (closed loop)
honeywell HZ432 zone panel
Why have you not changed the design of the Amana AMH inducer motor they fail at a year old, have been for 5 years and you are still giving me the same motor to replace it with. Make more of a robust...
i think i still have my trac pipe and wardflex cards somewhere, should send the to the Smithsonian :LOL:
does it have a communicating air to air H.p.?
you can program the new board without the chip if you have a communicating stat. I keep a CTK02 stat on my van just for this reason. i pull the connector off the old board...with it still connected. ...
this is very informative to a tech that hasn't worked on any microchannel units yet, thanks a bunch.
I have installed a few FUJI's and serviced somewhere around 20, also own one in my own home. we are also an AMANA dealer so we are pushing DAIKIN, but i much prefer fujitsu due to my familiarity to...
Board's lots and lots of boards. Other than that they are solid
I have had problems with the ptrap not having anything in it, it wont pull in the front cover plate switch and furnace fail to ignite. I found this more typical with the 3 and 4 year old units that...
I condemned 2 of them this week, the back cover plate where the primary and secondary hook up had BIG holes in them.
these are great and were just on sale a t Johnstone by me a few weeks ago.
Surge, go big or go back to the shop. it doesn't matter which one you get as long as you get one.
This is a great Flood for working in dark crawl spaces or just low lit areas
This is the...
I have been doing this 12 years and i don't even know what this is "hot wire anemometer" nor have i ever needed one...I don't think:)
i had this problem with one brand new out of the box and put on a intermittent pilot spark board and a new gas valve, homeowners are so much happier that they don't have to worry about the pilot...
help me understand you have the new board in it and it doesn't flash any codes? does the blower run at all with or without the fan switch "on"? and you disconnected the t-stat wire and jumped out r...
I love my UEI testers
I personally am a big fan of raypak,easy to work on and fairly easy to find parts for.
i just worked on one a few weeks ago, it amazes me how long things last when quality is stood highest priority.
that's a good furnace for a barn, just put a new filter and nozzle in it, adjust air and run it.
Did you hear about this on Free Beer and Hot Wings?
I love the expanding foam
I ran into this problem 2 weeks ago, found the black condensate lines that drop into the trap were cut to short, also found if they aren't cut down about an 1" the pressure switch won't always pull...
wow to pulling up an old thread...did anyone ever figure out the real truth to this debate? Now i am curious.
seems to me that this wouldn't work well in a residential application.
anyone here ever run into one of these systems? I have never heard of it but i am interested.
haven't seen the 17 yet but just did a startup on the 19 seer today.
we get paid from boots on to boots off if it is within 20 miles. anything over that we get paid from the time we leave to the time we are done with the job.
this is correct the board must see open before it see's closed.
communicating is amazing:.02: just wired a furnace, 19 seer heat pump, and t-stat today, :gah::gah::gah: what a mess that turned out to be.
TEDKIDD, what do you know about the new modulating...
I agree with this being a trane tech i wouldn't put less than a XL800 Deluxe. On another note in 10 years when a variable speed motor fails those are very costly, i mean VERY!!!
Trane, because i find them easier to service then others, shorter service calls make for a less expensive bill.