text:
, Volume 72, Issue 3, pp 307-319
First online:
Photosystem I from the unusual cyanobacterium Gloeobacter violaceus
- D. MangelsAffiliated withMolekulare Pflanzenphysiologie, Universität Bremen
- , J. KruipAffiliated withBiochemie der Pflanzen, Ruhr-Universität Bochum
- , S. BerryAffiliated withBiochemie der Pflanzen, Ruhr-Universität Bochum
- , M. RögnerAffiliated withBiochemie der Pflanzen, Ruhr-Universität Bochum
- , E.J. BoekemaAffiliated withBiofysische Chemie, Rijksuniversiteit Groningen
- , F. KoenigAffiliated withMolekulare Pflanzenphysiologie, Universität Bremen Email author
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Photosystem I (PS I) from the primitive cyanobacterium Gloeobacter violaceus has been purified and characterised. Despite the fact that the isolated complexes have the same subunit composition as complexes from other cyanobacteria, the amplitude of flash-induced absorption difference spectra indicates a much bigger antenna size with about 150 chlorophylls per P700 as opposed to the usual 90. Image analysis of the PS I preparation from Gloeobacter reveals that the PS I particles exist both in a trimeric and in a monomeric form and that their size and shape closely resembles other cyanobacterial PS I particles. However, the complexes exhibit a higher molecular weight as could be shown by gel filtration. The preparation contains novel polypeptides not related to known Photosystem I subunits. The N-terminal sequence of one of those polypeptides has been determined and reveals no homology to known or hypothetical proteins. Immunoblotting shows a cross-reaction of three of the polypeptide bands with an antibody raised against the major LHC from the diatom Cyclotella cryptica. Electron microscopy reveals a novel T-shaped complex which has never been observed in any other cyanobacterial PS I preparation. 77 K spectra of purified PS I show an extreme blue-shift of the fluorescence emission, indicating an unusual organisation of the PS I antenna system in Gloeobacter.
Reference tools
- Title
- Photosystem I from the unusual cyanobacterium Gloeobacter violaceus
- Journal
Photosynthesis Research
Volume 72, Issue 3 , pp 307-319
- Cover Date
- 2002-06
- Print ISSN
- 0166-8595
- Online ISSN
- 1573-5079
- Publisher
- Kluwer Academic Publishers
- Additional Links
- Topics
- Keywords
- antenna system
- electron microscopy
- fluorescence
- light harvesting
- photosynthesis
- plasma membrane
- Industry Sectors
- Authors
- D. Mangels (1)
- J. Kruip (2)
- S. Berry (2)
- M. Rögner (2)
- E.J. Boekema (3)
- F. Koenig (1)
- Author Affiliations
- 1. Molekulare Pflanzenphysiologie, Universität Bremen, 28359, Bremen, Germany
- 2. Biochemie der Pflanzen, Ruhr-Universität Bochum, 44780, Bochum, Germany
- 3. Biofysische Chemie, Rijksuniversiteit Groningen, 9747 AG, Groningen, The Netherlands


text:
Take a sneak peek at the new NIST.gov and let us know what you think!
(Please note: some content may not be complete on the beta site.).
Bioscience & Health News
U.S. Commerce Department Awards Small Businesses $3.3 Million for Innovation Research
Release Date: 07/06/2016
The U.S. Commerce Department's National Institute of Standards and Technology (NIST) today announced that 19 small businesses will receive nearly … more
Shape-Changing Enzyme Suggests How Small Doses of Anti-HIV Drug Might Treat Alzheimer's Disease
Release Date: 06/28/2016
For a promising pathway to treating Alzheimer's patients, "aim here." That's what National Institute of Standards of Technology (NIST) researchers … more
Department of Justice and National Institute of Standards and Technology Name Two Experts as New Members of National Commission on Forensic Science
Release Date: 06/16/2016
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A Call for Developing—and Using—Consensus Standards to Ensure the Quality of Cell Lines
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Mainstays of biomedical research, permanent lines of cloned cells are used to study the biology of health and disease and to test prospective … more
SF6 Shows Promise as Mercury Substitute
Release Date: 05/27/2016
PML's Weston Tew with the new triple-point cel more
NIST and Stanford Launch Long-Term Measurement Initiative to Boost the U.S. Bio-Economy
Release Date: 05/20/2016
STANFORD, Calif.—The U.S. Commerce Department's National Institute of Standards and Technology (NIST) and Stanford University announced today that … more
Newly Patented NIST Technique Creates Precisely Sized Nanocontainers Useful for Drug Delivery
Release Date: 04/07/2016
What if doctors could deliver anti-cancer drugs directly to tumors without making patients sick? Bringing this dream of targeted drug delivery … more
DNA-Encoded Circuits Made Easier, Faster, and More Measurable
Release Date: 04/01/2016
Researchers from the Massachusetts Institute of Technology (MIT), Boston University, and the National Institute of Standards and Technology (NIST) … more
NIST Develops First Widely Useful Measurement Standard for Breast Cancer MRI
Release Date: 03/23/2016
Researchers at the National Institute of Standards and Technology (NIST) have developed the first widely useful standard for magnetic resonance … more
Large Turnout for Funding Opportunity Briefing for DOC-Funded Manufacturing Institutes — Video Available Online
Release Date: 03/18/2016
On Tuesday, March 8th, 2016, the NIST Advanced Manufacturing Office (AMO) hosted over 200 participants for a Proposers' Day , where the attendees … more
Amping Antimicrobial Discovery with Automation
Release Date: 03/16/2016
The antimicrobial arsenal that we count on to save millions of lives each year is alarmingly thin—and these microbes are rapidly evolving … more
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The new breast phantom consists of two components. The one at more
Study Highlights Need for Better Characterized Genomes for Clinical Sequencing
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A new study that assesses the accuracy of modern human-genome-sequencing technologies found that some medically significant portions of an … more
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Alexandra Rodriguez demonstrating the broken contact style of data logger. Recipients of an annual flu shot may be surprised to learn that there … more
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Release Date: 02/11/2016
When it comes to early diagnosis of Lyme disease, the insidious tick-borne illness that afflicts about 300,000 Americans annually, finding the … more
Related Links in:Material Measurement LaboratoryPhysical Measurement Laboratory
General Information:
100 Bureau Drive, Stop 1070

subset: commoncrawl


text:
Mr. Bernice On Phone To Square Service
I have been waiting for my refund for months. I fianlly get an email saying that its in my favor and not funds yet cant get a customer code please call 917-935-3685
Thanks please in this lifetime.
A merchant that uses Square sent me a refund July 11, 2016 and I have not received the funds yet. The merchant is Hanics America and the refund is $540. 00. As always I can not get in touch with anyone at Square, I have tried calling and emailing with no response. Can anyone help me out here? It would be appreciated. Hanics says the funds have already been taken from their account.
We are getting emails saying that we owe Square over $3000. We do not have a Square account, we do not use it in our business, and your phone number only works for existing accounts. We owe you nothing, and if you call us, we will need your account number to speak to you.
Ms. Dahria Malchevskaya On Phone To Square Service
Mr. Leonardo Neira On Phone To Square Service
Hi my name is Leonardo Neira . Yesterday I took a costumer from Cambria hotel to jfk)terminal 4) at 6:30 pm. I charged 145 plus taxes. But his card just had 150 and he told me the rest I will pay you cash. He signed, but I dint get my money in the bank.
Ms. Ruth Peterson On Phone To Square Service
425-330-4802 I would like a phone call please. I would like to remove my email address from you database. I must have given it to a vendor in the past. Now if a vendor uses Aquare, they do not even ask me regarding my receipt, they just email it to me. Pleas call and we discuss this.
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Mr. John Marsh On Phone To Square Service
Unable to speak with a live person and not getting or understand the web site results. I need help or I'm not going to do business with a company that doesn't think enough of me to talk with me.
Reginald On Phone To Square Service
I need to get a contact phone number for square my money is still pending and I want to know why. Please send me a contact phone number
I charged someone to my Square devce 180$, it should come to my account, but its showing debt in my account please i need money resolve my this problem 5712086624 Ali Chohan.
I recently visited a merchant who uses Square to accept payment for services. The problem I'm having to which I'm not getting anywhere in receiving any help. I charged using my MC and this charge generated an electronic receipt. The problem, the receipt was not sent to me, the person who charged on it. The receipt was sent to my spouse who is an authorized MC user on my account. I need to contact someone who can help me understand why my spouse received a receipt for my charge.
Mr. Huguens On Phone To Square Service
Hello. I recently got your device. I know you charge 2. 75 percent per transaction when we swipe the credit card. But do you charge the same when we enter the c. C manually. Or if not, what percent do you charge when we enter the credit card info in the system manually. I'm looking forward your answer.
Ms. Deb Pendergraft On Phone To Square Service
I need to ask a question about personal SS number used for a business that is not mine. Please call me 9185192888. Deb Pendergraft.
Square account United Limos has charged one of my customers $200. 00. We are United Limousine and Charter, Inc. This is a fraud case. Call me as soon as possible at 562-577-7451.
Ms. Haley On Phone To Square Service
I need to talk to somebody regarding my square program not working on my iPad and iPhone, but only an automated message comes up, tried many times, couldn't get to talk to a live rep.
Ms. Kitty Burkey On Phone To Square Service
I cannot get through to a human being for any help. I believe my reader is no longer functioning. I am paying more fees because the card reader is no longer functioning. I am trying to see if they can help me test it. I an no spring chicken, but have had success with the reader in the past. I am not happy i have to pay additional fees because i didn't know the reader had stopped working, it has certainly not been abused. I would appreciate your help in resolving my issue. I am frustrated. I need a human to contact me.
To whom it may concern,
I am having trouble with this weekend transaction, on July 18,2014 my gross sale was $225 and July 19,2014 my gross sale was $170 which bring us to a total of $395.
Square had sent only $320. 92 to my account. Can you please investigate this matter for me? thank you. Please call 410-302-3703 or email me.
Ms. Ginny Nadler On Phone To Square Service
I changed my email address and bank account for deposits on or about the 20th of June. I received a confirmation that my information was changed. I used my square for deposits and i have received confirmation that $651. 58 has been deposited. The old account number longer exists for deposits and the account i requested the change has received no deposit. When i attempt to get into my account on line the new email address does not work nor does the old. I need this money immediately.
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Ms. Phyllis Mcgough On Phone To Square Service
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Ms. Jennifer Schaffner On Phone To Square Service
A customer is trying to pull an insurance scam, when their insurance denied them they tried to file a false report saying my company CNJ Appliance did not install the almost $800.00 part we did indeed purchase and install. We have sent all documentation and disclosures that this is a non-refundable part. The customer was well aware as well. Despite the fact that we have a legally binding contract your company not only refunded money he was not legally entitled to, but you went into my bank and effectively took all my other customers money to give it to a scammer.We want no part of this man's insurance scam nor are we legally responsible and we have the signed waiver to prove it. I need a phone call today at 2819748862.
Ms. Emily Cayson On Phone To Square Service
hi my name is Emily Cayson I have a spuare reader and I processed a charge today 10-4-2013 around 2pm mountain time for 35 dollars, no email was sent saying I accepted this and what my new square balnce is. It should be around 80.00 as of today. My company name is Doggy Clips in Nampa ID, 83686. My email address is firstname.lastname@example.com Please contavt me back as soon as possable. Thank you
I have been trying to get in contact with a representative to reconnect my Square to my new phone, for the past few days. I need to speak to a live person because when I put in the security question answer to the ' what street did you grow up on? ' it says that my correct answer is wrong I've missed out on several transactions and need for a rep to call (404) 429-6685 immediately
I need to buy a Square, but I speak Spanish. Is possible speak with a person that speak Spanish please. I living in Miami. My phone number is: (786) 394 3267. My name is Maria Bautista. Please call me. Thank you.
Mr. Tim On Phone To Square Service
My name is Tim Henry, I am a square card reader member using a Samsung s three android phone, my question is how do I set up reacuring payments from my customers.
I am also having dificulties signing in, as my primary email address email@example.com no longer exists.
Need help asap
Ms. brittanie On Phone To Square Service
Had transactions on 9/20 in almost 500 dollars. Customers werw all charged and my square accoumt showed transactions, but I never received payment. Found out somehow my bank account was unlinked. Linked it back and now all of my transactions have disappeared from my account. I need someone to call me. 813 957 8734. The fact that there is not someone to speam with is absolutely ridiculous considering you are accepting payments for businesses.
Ms. Laura On Phone To Square Service
My square app is not working. AT&T says it is a software problem with square. I've reinstalled and am getting frustrated. I need to speak with a person or will be using a different service. I've got through contact us online and left very detailed message as to what the app is doing and the responses are ridiculous, as if the person answering does not even understand.
My number is 903-293-0889. I need a call today or its see ya later, sorry. Trying to run a business;(
Laura McLean
Mr. Omer On Phone To Square Service
I have been receiving pop a lock invoices to my e mail at email@example.com
Please disconnect my service and correct the issue .
Mr. Graphic Designs On Phone To Square Service
I am having a problem with swiping cards using my card reader. I have requested a new card, but have not received one yet; therefore, I have to pay the higher rate. Please
Mr. DAVID On Phone To Square Service
I HAVE CALLED SQUARE THEY DO NOT HAVE ANY ONE TO ANSWER, I WANT A CALL BACK TODAY OR I am FILEING A FEDERAL COMPAINLT. DAVID 239-273-5191
Mr. DAVID On Phone To Square Service
hi I signed up for square I hard a customer put $4000 dollars on a new account and square is holding my money for 90 days this is bullshit and than they are holding my account what kind of company is this ?David Bailey 239-273-5191
Ms. kaitlin menger On Phone To Square Service
My reader has not shipped and was set to be here on August 12th. It is now the 21st....everything I look at I cant find anywhere to have a new one sent to me.
Ms. Lindie On Phone To Square Service
I changes bank accounts and am trying to use my square for a new account setting it up its asking me email , etc then tells me e mail already being used how do I use this square reader for a new bank account
Ms. Valerie On Phone To Square Service
I ran a charge for $3400 and it was credited back as a refund the day before it was processed. The client was charged and no refund showed up om his statement . Someone has $3400 in there account and it not me. I am waiting two days now to hear back from someone at square. This is an unprofessional way to handle customer service. I am assuming this will be straightened out, but it's very frustrating not to be able to talk with someone directly.
Ms. KAROL SHAHID On Phone To Square Service
MY HUSBAND DELETED MY SQUARE ACCOUNT FROM MY PHONE AND I CANT REMEMBER MY SECURITY QUESTION TO LOG BACK IN. I HAVE TRIED EVERYTHING TO CONTACT SQUARE, but CANNOT GET HELP! I GUESS I WILL HAVE TO GET ANOTHER READER FROM A DIFFERENT COMPANY, ONE THAT ACTUALLY HAS A LIVE PERSON TO HELP IF THIS EVER HAPPENS AGAIN. THANKS SQUARE FOR NOTHING.
Mr. Jason Jarvis On Phone To Square Service
my card was initially charged 2000 dollars, but transaction was cancled and now that money is on hold within your company. This card is linked to my bank account and it was taken out of my checking account.
What do I do
Mr. Mark On Phone To Square Service
DO you guys have a extension pin for the square. My type of cell phone case which is a griffin will not allow it to seat properly into the jack. I would like to keep the case i have. Sincerely your customer: Mark J Talbert ps: cell case is difficult to remove on and off for each transaction.
Mr. sadruddin On Phone To Square Service
Hi, my name is sadruddin g hussain. I drive a cab and use square for any cc transactions. Yesterday a transaction went through three timesvs once. Is there anyway you guys can redund the transactions? Please let me know what I need to do. If you can call my daughter at 312-351-0952 her name is sabeen.or if you can respond at email@example.com that would be great.thanks.
Mr. Tony Duran On Phone To Square Service
Can you call me? I have questions before I begin using your services. I will be here by the phone for another ten minutes. It's 1:05 Central time.
Thank you,
Ms. Camille Bruce On Phone To Square Service
The reason for this message is because I am a square card user and I have not received the payments from the transactions that I completed. Can you assist me in order to receive my payments? My number is (702)-272-5064 and my user name is Even Photography. My routing number is connected to MidCountry Bank. Please help me resolve this issue.
Ms. Stacey mcelheny On Phone To Square Service
I changed my account first week of July. It didn't link cause I didn't verify account correctly. There's $359 that didn't go to either account
Mr. ramon espinoss On Phone To Square Service
my credit card had to be chained because of fraud/ my old bank ws citi bank/new bank chase
my last four on my SSI IS 3476 MY NEW CHASE LAST four IS 9126
Ms. Alma Rodriguez On Phone To Square Service
I had a customer dispute a transaction and It was paid back to them without a investigation of any type. Customer received a service and just because he was going out of the country and need money filed this claim. I need to speak with a actual person ASAP...704-649-0556
Mr. william jackson On Phone To Square Service
Yall are holding my payment my customer has told me the amount has been deducted I need to speak to someone as soon as possible 8328895765
Mr. LUIS MARMOL On Phone To Square Service
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Need to talk to a human my number is 7275124101 . I made deposits to a bank acct that Ihad forgotten I closed . I then linked my current account . Got an email from Square stating monies were sent to previous acct . Want to know if my monies will now be deposited to current linked account .
Ms. Moe Mosher On Phone To Square Service
I accepted a $65 payment on Sat. (6/29) @ 9:38am that did not seem to go through. The customer, however, did receive a receipt. Please contact me regarding this at 716-208-9981.
Moe Mosher
Mr. Buzzard On Phone To Square Service
I called the phone number and it said go to the website. I need to speak with a representative now! Talk about shitty customer service. This takes the cake! Should be called customer non-service!


text:
Tie-Down keeps valuable cargo where it belongs
Works just like a standard tie-down, but the handle removes easilyafter you tension your load. All you need to do is depress the spring-loaded release to remove the handle to deter unauthorized removalof cargo. Heavy-duty yellow polyester strap is equipped with durablezinc-plated steel components for longer life. Use with trucks, flatbedsor trailers. 3,000-lb. breaking strength, with a 1,000-lb. working loadlimit. 1-1/2"W and 15'L. Imported.


text:
Media Don't Judge A Sitt By Its Column?
posted by December 27 at 9:56 AMon
The Stranger's morbid appreciation of the Seattle Times "Girl About Town" Pamela Sitt is well-documented.
So imagine my surprise when I read this news report, which features exactly none of the labored whimsy and tortured prose that's distinguished Sitt's column since its inception.
Let this be a lesson to us all.


text:
A crash involving a bus carrying children and a large truck killed 18 people in a Moscow suburb Saturday, authorities said.
At least 61 others were injured, Russian health officials said.
Dozens of ambulance crews responded to the accident in the village of Oznobishin, state-run news agency RIA Novosti reported.
Helicopters will fly in medical personnel.


text:
Video Industry News Video Company News Technology Industry News Technology Company News Novelty Industry News Novelty Company News Gay Industry News Gay Company News Legal Industry News All Company News
Released: Oct 01st, 2006
Running Time: 143
Company: Lezzie Films
Distribution Companies: Filmco
Cast: Tayla (I), Alaina, Angelina (I), Hunter (I), Alana Evans
Critical Rating: AAA
Genre: Pro-Am
Fans of soloing and small natural boobs will appreciate this amateur title, which delivers exactly what it promises. Five hot women with varying enthusiasm levels take us through three scenes each of fingering and toy play. The best of this bunch are the blondes: second babe Tayla, using a rabbit vibrator and lots of baby oil, cums her brains out in her first scene, and Angelina brings up the rear with some steamy come-ons to the camera. She's the most energetic, making more of a performance out of her scene.
The three short scenes per actress idea, instead of adding variety, is really overlong and unnecessary, and the action is absolutely standard solo toy play – no anal or d.p. Still, the camerawork is good, particularly the close-ups in exotic brunette Alaina's solo.
Retailing: One copy in the amateur section should be sufficient.


text:
Thank goodness New York City Mayor Michael Bloomberg deeply understands the meaning of free speech and what it does and doesn't look like, or else his forced decampment of Occupy Wall Street's Zuccotti Park HQ would look like a completely overstated and egregious abuse of political power. Also, as Stephen Colbert points out in this "Colbert Report" clip from Tuesday's show, at least one guy in Berkeley knows how threatening the protesters' practice of linking arms actually is. —KA
'The Colbert Report':


text:
There is no country on earth whose major population is both well-educated and impoverished. A strong educational foundation is the path out of poverty. GSSG is dedicated to responding to the heartfelt desire of the Guatemalan people for an improved educational system. GSSG works with partners to provide the resources needed to achieve that goal.
Priscilla Otto Leadership Award
The Guatemalan Student Support Group [GSSG] has announced that the winner of the inaugural Priscilla Otto Leadership Award is Luis Fernandez. Luis, who is attending medical school in Guatemala, will receive a $500 scholarship.
Facebook Page: The Guatemalan Student Support Group now has an official Facebook page! It is as follows: http://www.facebook.com/gssg.usa. If you are on Facebook, please "like" this page by clicking the Like button under the photo in order to spread the word and get updates about GSSG on your Homepage within Facebook. Once you like GSSG, you can easily ask your "friends" to like it by clicking buttons to invite them. Let's get GSSG out into the mainstream!
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Email: email@example.com


text:
by Ben Armbruster Jan 9, 2014
Democrats have yet to flock en masse to support an Iran sanctions bill sponsored by Sens. Mark Kirk (R-IL) and Robert Menendez (D-NJ).
by Ben Armbruster Jan 6, 2014
Experts led by former U.S. Ambassador to Iraq and Afghanistan Ryan Crocker ask that Sens. Robert Menendez (D-NJ) and Mark Kirk (R-IL) not move forward with their Iran sanctions bill at this time.
by Hayes Brown Dec 19, 2013
Ten Senate chairs have called on Senate Majority Leader Harry Reid (D-NV) to consult them before any new sanctions legislation hits the floor.
by Ben Armbruster Dec 2, 2013
Senators pushing more sanctions on Iran say allowing Iran to enrich uranium violates United Nations resolutions. Experts disagree.


text:
I'm new to rails and git. I have a rails application under ..../myapp that I would like to deploy using capistrano. I followed the steps described in "Agile Web Development with Rails". To put the application under version control, I did the following:
1) Put my application under version control:
$ cd myapp
$ git init
$ git add .
$ git commit "..."
That all went fine, it created a .git directory under myapp/
2) Created an empty repository on the server:
$ mkdir -p ~/git/myapp.git
$ cd ~/git/myapp.git
$ git --bare init
$ Initialized empty Git repository in /root/git/myapp.git/
3) Created a public key
$ ssh-keygen -t rsa -C "email@example.com"
$ Your identification has been saved in /root/.ssh/id_rsa.
$ Your public key has been saved in /root/.ssh/id_rsa.pub.
$ The key fingerprint is:
$ d2:16:76:e0:4c:71:de:de:4b:d3:16:94:cc:d7:65:11 email@example.com
$ cat ~/.ssh/id_dsa.pub >> ~/.ssh/authorized_keys
4) From my application directory: created the path to the server and push the code
$ git remote add origin ssh://me@host/~/git/myapp.git
$ git push origin master
Now I'm being asked to enter my password, after that:
$ fatal: '~/git/myapp.git' does not appear to be a git repository
$ fatal: The remote end hung up unexpectedly
It might be worth noting that this all happens on the same server, a RHEL 5.7. This is something that confuses me because I basically ssh from my server onto my server. But apparently that's how capistrano does it, even if the application_development and the svn repository are on the same physical machine.
@ manojlds:
$ GIT_TRACE=2 git push origin master
$ trace: built-in: git 'push' 'origin' 'master'
trace: run_command: 'ssh' 'me@host' 'git-receive-pack '\''/root/git/myapp.git'\'''