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Moderate improvements in cardiac performance have been reported in some clinical settings after delivery of bone marrow mononuclear cells to patients with cardiovascular disease (CVD). However, mechanistic insights into how these cells impact outcomes are lacking. To address this, the NHLBI Cardiovascular Cell Therapy Research Network (CCTRN) established a Biorepository Core for extensive phenotyping and cell function studies, and storing bone marrow and peripheral blood for 10 years. Analyzing cell populations and cell function in the context of clinical parameters and clinical outcomes, after cell or placebo treatment, empower the development of novel diagnostic and prognostics. Developing such biomarkers that define the safety and efficacy of cell therapy is a major Biorepository aim.
Cell therapy has emerged as a potential treatment for CVD states ranging from refractory ischemia and acute myocardial infarction (AMI) to left ventricular dysfunction and heart failure (HF) (Figure 1). Unlike current treatments aimed at increasing or preserving myocardial performance, cell therapy has the potential to enhance repair of underlying myocyte, matrix, and/or vascular damage. While few insights about cell function and its optimization have materialized, experience suggests autologous cell therapy is safe and offers potential for moderate improvement in cardiac performance (1-6). However, optimal cell preparation, handling and delivery have not been established (7, 8).
The most prevalent cell population studied in cardiovascular clinical settings is the bone marrow mononuclear cell fraction (BM-MNC) that contains stem and progenitor cells. Benefit has been suggested with autologous BM-MNCs for acute ST segment elevation myocardial infarction (STEMI) patients after percutaneous reperfusion (1-4, 6, 16-22), but in other patient cohort results have been mixed (Figure 1). Covariates suggested to impact effects of transplanted cells on left ventricular ejection fraction (LVEF) include cell isolation techniques, cell phenotype (i.e. types of cells present), and functional viability of these cells (e.g. ability of cells to form colonies in vitro) (8, 15). The Cardiovascular Cell Therapy Research Network (CCTRN) was established by the National Heart, Lung and Blood Institute (NHLBI) to develop, coordinate, and conduct collaborative trials evaluating effects of stem/progenitor cell therapy on CVD (23). The purpose of this article is to describe the CCTRN Biorepository Core Laboratory, which was designed both to store samples, and to begin to evaluate some of these covariates.
Details of the trial protocols are published elsewhere (21-24). All studies were conducted according to CCTRN guidelines with approval of Institutional Review Boards of each participating institution, and each patient provided written informed consent.
All patients undergo a standardized bone marrow (BM) aspiration and venipuncture on the day of treatment. Details of cell handling and processing are published elsewhere (25). For patients who provide informed additional consent, after preparation of the treatment product (cell or cell-free) the remaining mononuclear cells and a peripheral blood (PB) specimen are sent to the Biorepository for storage and phenotypic analysis (University of Minnesota) and for functional analysis (University of Florida). The day of BM harvest and study product delivery is designated Day 0. PB is collected at predetermined time points before (Day 0), and after study product delivery (Figure 2).
At each time point (Figure 2), 20mL of PB is obtained in two vacutainers (BD Biosciences, Bedford, MA) containing EDTA following venipuncture; tubes are mixed well, and both BM and PB stored at 4°C until packaged shipping. Prior to shipping, tubes are wrapped in absorbent material and bubble wrap, placed in a Biohazard bag, and shipped with two refrigerated (4°C) packs and appropriate forms for next morning delivery. Upon arrival, PB samples are treated with ammonium chloride potassium (ACK) standard red blood cell lysis buffer, centrifuged at 50×g for five minutes at 4°C, and the supernatant aspirated. The pellet is resuspended in ACK lysis buffer, centrifuged at 50×g for five minutes at 4°C, the supernatant aspirated, and cells washed with PBS twice, before resuspension in FACS buffer containing PBS + 2.5% Fetal Bovine Serum. The number and viability of resulting nucleated cells is determined in an automated cell counter using the Guava ViaCount assay (Millipore, Billerica, MA). One to five million cells are stained using antibodies raised against human CD45, CD34, CD31, CD133, VEGFR2, CXCR4, CD3, CD11, CD14, CD19, and their corresponding isotype control. After incubation, cells are washed twice with, and resuspended in FACS buffer for FACS (LSRII cytometer, BD Biosciences, Bedford, MA). Data are analyzed using FlowJo software (Tree Star Inc, Ashland, OR) with standardized guidelines for gating.
To ascertain maximal cell viability during shipping, a shipping study was undertaken. Overall PB cell and subpopulation viabilities were quantified after shipping of samples with either refrigerated (4°C) packs or frozen packs. As a control, samples were not shipped but stored at 4°C overnight or left at room temperature. Room temperature storage resulted in with widely disparate sample viabilities (57.7-92.5%) and was not considered optimal for these samples, and thus was not used as a control. Optimal time-to-processing of PB was determined by evaluating viabilities by FACS-sorted PB at 0, 24, 48, or 72 hrs after harvest. Once optimal shipping conditions were determined, SOPs were generated and distributed to cell processing facilities. Standardized sample shipping containers, cold packs and wrapping materials were also provided. After distribution of materials, optimal sample shipping conditions were addressed by phone calls with network investigators and research coordinators. If BM or PB sample viability from a given center drops below 80%m for a single sample, the center is immediately contacted and shipping procedures reviewed.
On Day 0, 30mL of blood is collected in vacutainers containing EDTA following venipuncture as described above. BM and PB mononuclear cells are analyzed for viability using trypan blue staining (Figure 3A) (Cellometer AutoT4, Nexcelom Bioscience, Lawrence, MA). Cells are reserved for endothelial and mesenchymal colony assays. When available, additional BM cells are processed for enrichment of the CD34+ stem and progenitor cell subset by immunomagnetic selection (Miltenyi, Auburn, CA). These CD34+ cells are then tested using assays for migration to stromal cell derived factor 1 (SDF-1). The CD34+ cell population is used as a surrogate for stem and progenitor cell activity in the entire BM-MNC fraction because use of the distinctly heterogeneous population of BM-MNCs generates highly variable migration data that would be difficult to interpret in the context of the CCTRN trials, which seek to recruit less than 100 subjects per trial.
For endothelial colony assays, mononuclear cells are first plated in endothelial growth media-2 (EGM-2, Lonza, Walkersville, MD), plated on fibronectin-coated dishes (BD Biosciences, Bedford, MA) and incubated at 37°C, 5% CO2, > 95% humidity for 48 hrs. Non-adherent cells are removed for colony forming unit Hill (CFU-Hill) assay and adherent cells are trypsinized for endothelial colony forming cell (ECFC) assay, as previously described (26, 27). Media is exchanged every other day and number of colony forming units, types of colonies and confluence are recorded on days 7, 14, 21 and 28 (Figure 3B & 3C). CFU-Hill colonies are defined as a central core of round cells with radiating elongated spindle-like cells at the periphery. ECFC colonies are defined as circumscribed monolayers of cobblestone-appearing cells.
For mesenchymal stem cell colony assay, also known as the CFU-F assay, three concentrations of BM-MNCs and PB-MNCs are plated in Mesencult serum and cytokine containing media (STEMELL Technologies, Vancouver, CA) according to manufacturer's instructions. Number of colony forming units, type of colonies and confluence are recorded on days 7, 14, 21, and 28 (Figure 3D). CFU-F colonies are defined as circumscribed clusters of spindle-like cells typically 1 to 8 mm in diameter.
To evaluate hematopoietic stem and progenitor cell migration to SDF-1, CD34+ cells are isolated from BM cells using immunomagnetic microbeads conjugated with anti-CD34 antibodies (Miltenyi Biotec Inc., Auburn, CA) and automated magnetic selection (AutoMACS, Miltenyi Biotec Inc.). These CD34+ cells are tested for migration to SDF-1 in a modified Boyden chamber, as previously described (28).
At each time point, 10mL of blood are collected in vacutainers containing Heparin Sodium and mixed properly. Tubes are centrifuged within 30 min at 190×g for 10 minutes at 4°C, the top plasma layer is carefully removed for cytokine analysis, without disturbing the middle leukocyte layer. Plasma is aliquoted into cryovials kept at 4°C. For future DNA/RNA analysis, the leukocyte layer is transferred to a separate cryovial avoiding significant erythrocyte contamination. Cryovials are placed in a -80°C freezer within 30 minutes for storage until shipment on dry ice to Biorepository Cell Phenotyping and Storage Core Laboratory (UMN) for centralized storage at -86°C. All samples are handled and disposed of following institutional guidelines, with universal precautions at all times.
In addition to plasma and leukocytes, BM and PB from CCTRN subjects are shipped to the Biorepository Cell Phenotyping and Storage Core Laboratory for FACS profiling and long term storage. After BM and PB samples are analyzed by FACS, remaining cells are frozen in 10, 25 and 50 million cell aliquots (50 million cells per mL) in FBS and 10% DMSO according to CCTRN SOPs. Cells are slowly frozen to -80°C in a isopropanol bath apparatus for 24-48 hrs, and transferred to liquid nitrogen tanks for long-term storage (-175°C). Cells are stored for at least ten years so that if any safety questions arise, the initially harvested samples can be made available for analysis. No samples received at the Biorepository Core Laboratory are returned to the sites for clinical application. Remaining sample above those needed for safety studies are available for ancillary studies once they are approved by the CCTRN.
Shipping with refrigerated (4°C) packs maintained both cell number and overall viability at levels similar to control samples stored at 4°C for 24 hours (Figure 4A). The temperature of the samples was: 1) 8°C for 4°C stored samples, 2) 7-14°C for samples shipped with refrigerated packs, 3) variable for samples shipped with frozen packs, usually <5°C, and 4) 19-22°C for room temperature samples. Specimens shipped with frozen packs showed a significant decrease in viability compared to samples shipped with refrigerated packs (p<0.05, Figure 4A), and a significant decrease in TNC per mL of blood (p<0.0001, Figure 4B). The impact of frozen packs was less pronounced when packs were smaller (data not shown). The relative percentage of cell subpopulations determined by FACS (progenitor or inflammatory cells), showed that samples shipped with refrigerated packs were least variable and mostly within a ±10% variability that can be expected for FACS analysis. Specific rare populations (AC133+, CD34+, and VEGFR2+) showed greater variability because changes of just a few cells, result in large relative changes. Samples shipped with frozen packs, or stored at room temperature were more variable for most subpopulations (Figure 4C). Though Seeger et al. showed that BM samples stored at room temperature were more functional (8), we found that PB became sub-optimal when temperatures of the samples exceeded 21°C (data not shown). Based on these data, all samples are routinely shipped with 4°C refrigerated packs for overnight delivery, which maintains the PB at temperatures that are least detrimental to the sample.
Cell viability was highest 24 hours post-draw, and decreased over time (Figure 5A). TNC number per mL of blood, decreased at 48 and 72 hrs post blood draw due to clumping or lysis of cells (Figure 5B). Both TNC number and viability were significantly reduced in samples 48 and 72 hrs post-draw (p<0.01 and P<0.0001, Figure 5A and 5B).
Progenitor cell populations that contribute to injury and repair were quantified in PB at increasing times after blood draw. All populations except CD19+ changed by less than 10% in the first 24 hrs (Figure 5C). By 48– and 72 hrs, the relative numbers of several subpopulations were significantly changed. (Figure 5C). Based on these subpopulation changes over 72 hours, shipping SOPs were developed for overnight courier delivery. Most samples arrive at the Biorepository Cell Phenotyping and Storage Core Laboratory within 24±2 hours from draw and are processed immediately. Occasionally, due to various circumstances, samples are processed later, and this is recorded.
BM-MNCs and PB from CCTRN subjects were also shipped to the Biorepository Cell Function Core Laboratory in Florida. Specimens were received in the Cell Function Core Lab within 30.4 ± 17.9 hours from time of harvesting. Cell viability was 84% ± 8% for BM-MNCs and 83% ± 11% for PB-MNCs. We found that the percent of both non-viable BM-MNCs and PB-MNCs did not correlate with the shipping time (Rho=0.04 with P=0.7283 for BM, and Rho=-0.04 with P=0.7147 for PB). The scatter plots of raw data (before transformation) show the same results with the fitted flat regression lines (Figure 7). In a regression analysis there is no bias introduced by uneven distribution across the predictor (X-axis). These data indicate that the shipping time within our established Shipping SOP had an insignificant effect on cell viability. Thus, variability in results from BM and PB specimens received in the Biorepository Cell Function Core Lab is likely related to clinical and biologic factors rather than time out of body.
A significant network "learning curve" existed relative to how best to store and ship both BM and PB samples (Figure 6). This was evidenced by low viabilities and longer draw-to-delivery times for samples from early in our CCTRN experience compared to samples from later in our experience. Sample viability was similar among the centers with the exception of one site for which same day courier delivery was used in place of overnight shipping in which case viability was increased.
A qualification of the Biorepository Cell Phenotyping and Storage Core Laboratory is long-term cell preservation, and thus evaluation of the impact of freezing on cell phenotype is important. Comparison of BM and PB cells before and after freezing showed that recoveries ranged between 32-60% for PB-MNC and 41-55% for BM-MNC. Whereas stem, progenitor, myeloid and T lymphoid cells remained well preserved with excellent post-thaw viability, the B lymphocyte subset which made up the majority of collected MNCs was primarily responsible for the overall lower post-thaw viability (Table 1).
One of the most valuable assets in the CV field is the Framingham Heart Study data set from which several important diagnostic and prognostic markers have emerged over the past 60 years (29). Yet to-date, it is not possible to correlate the important prognostic and diagnostic data gathered from Framingham patients with one of the more potent therapies envisioned – stem cell-mediated recovery after myocardial or vascular injury. The heterogeneity of clinical outcomes after cell therapy (Figure 1) suggests that important variables in cells, target tissue, or both exist. Understanding if those variables are a result of clinical disease, patient demographics (e.g. age, sex) or cell processing is critical if the field of cell-based therapy and diagnostics is to advance. For example, others showed that storage of patient BM cells over 24 hours could alter function (8); however, our preclinical data suggest that sex and age impact phenotype and function of BM-MNC (30, 31). By evaluating the number and function of cells in patients undergoing CV cell therapy, we have an opportunity to generate a reference database for patients undergoing such therapy. Understanding relationships between cell phenotype of BM-MNC, circulating cells, and function is crucial to optimize future cell therapy treatments. In addition, by storing BM, PB and serum, the CCTRN has the ability to bring both relevant clinical and specimen biologic data together in order to address patient safety questions.
In addition, our results show that BM and PB can be collected at various sites, shipped, and analyzed in a central core – making evaluation of cell phenotype and potency feasible. Preliminary findings suggest progenitor cell populations differ both in composition and function as time elapses after AMI.
Our work demonstrates that proper education of site coordinators on a validated shipping SOP, appropriate documentation, and ongoing communication between the core lab and clinical sites is essential for reproducible, consistent data over time. Since analysis of FACS data by gating for subpopulations can be subjective, standardized guidelines are required for data accuracy. Likewise, evaluations of colony formations and cell confluence should involve a visual atlas to verify accuracy of assessments. The development of cryopreservation methods that allow phenotypic and other analyses of frozen samples with minimal cell loss and minimal changes is of longstanding need, and is being pursued by the Biorepository Cell Phenotyping and Storage Core Laboratory.
Central core labs do not come without a cost. They can be expensive; and burdens imposed by complicated shipping procedures are considerable. However, in a centralized core lab with SOPs tailored to the studies, you have the advantage of fewer personnel to reduce operator variability, the quality of the data are superior, less variable, and more precise, and interactions with a centralized data coordinating center for statistical analysis are simplified. In addition, centralized core labs have access to a variety of data for controls or to patients with various other cardiovascular diseases to which study data can be compared and contrasted.
Creation of a Biorepository Core Laboratory for characterization of biologics is feasible in a network. Within the US, samples can be shipped nationally and physiologically relevant measurements made in a timely manner from shipped samples. To maximize the utility of these cell samples they should be shipped at 4°C to avoid freezing, and elapsed time from blood draw to processing should be 24-48 hours. Adhering to these established SOPs, reduces variability in cell phenotype and function assessments.
Stored biologic samples from cell therapy clinical trial patients provide a safety net should therapeutic questions occur. They allow for future analyses as new assays are developed and hypotheses generated, and provide a platform to address novel questions arising in treatment of cardiovascular disease. No samples received and evaluated in the Biorepository Core Laboratory are returned to the clinical sites or used for subject treatment. Instead, research samples are used to characterize injected cell phenotype and function, and stored for future Network-approved ancillary studies and safety analyses, if needed.
The authors wish to thank the CCTRN site principal investigators, the site research coordinators and the patients. The authors also wish to thank Paul Champoux at the University of Minnesota for his flow cytometry support and advice.
Funding Sources: This study was supported by research grants from the NIH NHLBI (U01 HL087318-01) and by the Production Assistance for Cellular Therapies (PACT), N01-HB-37164.
The University of Minnesota flow cytometer, a Masonic Cancer Center shared resource, was supported in part by grant NIH P30 CA77598.
Authorship: The authors are solely responsible for the design and conduct of this study, all study analyses, the drafting and editing of the paper and its final contents.
Conflict of Interest Disclosure: The authors declare no conflicts of interest.
Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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Tony Guzman's curator insight, February 16, 1:03 PM
This article and screencast shares how you could share video without having to upload it on YouTube.
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These are wonderful suggestions for engaging students with iPads that offer opportunities to create in a multitude of intelligences..
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1. Gallery Walks/Chalk Talks
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Each is explained in detail in the post. Do you have ideas on how movement can be incorporated into lessons? If so, share them in the comment section.
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A few great ideas for critical thinking. I particularly liked the activity called "telephone" (not the listening game). Teachers can use all the strategies they can to help their students think better!
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Great to have a summary aimed solely at educational functionality, thanks for sharing!
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Central Access Reader -A FREE screen reader that reads Math accurately, is going to help so many students and individuals who struggle with literacy, processing, dyslexia, dysgraphia, ADHD, and other print disabilities, as well as those who are visually impaired or aging and simply benefit from hearing the math aloud.
"Spencer Graffe, a senior in Central Washington University's Computer Science Program, has helped develop Central Access Reader (CAR), a free computerized text-to-speech reader program that has attracted attention of institutions across the nation.
Marshall Sunnes, Central Access program coordinator and Wendy Holden, Disability and Accessibility Consultant, collaborated to determine what the program should be, how it should look, and what features were most important to include. Graffe had the skills to make it happen.
The program, developed as a tool to assist students with print-related disabilities—those with visual impairments, dyslexia, attention-deficit disorder, or other conditions—reads documents that other text-readers can't handle, especially those with equations or symbols." Watch the video demonstration, and download CAR for FREE here:
**Alyssa Marinaccio is also the Assistive Technology Coordinator at Keene State College.
***Visit Assistive Technology for Education, LLC for assistive technology assessments, trainings, and consultation to access education and employment.
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Diana Petschauer's comment, July 14, 2013 7:06 PM
And Fast Fig app-free in the Chrome Browser: https://chrome.google.com/webstore/detail/fastfig/bogefeobnkbodnohkifkjfdipjmdljkd?utm_source=chrome-ntp-icon
Diana Petschauer's comment, July 14, 2013 7:05 PM
Diana Petschauer's comment, July 14, 2013 7:06 PM
And Fast Fig app-free in the Chrome Browser: https://chrome.google.com/webstore/detail/fastfig/bogefeobnkbodnohkifkjfdipjmdljkd?utm_source=chrome-ntp-icon
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The other method is to put a numeric keypad on the drive itself that lets the user to unlock it with a PIN. .......
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Absolutely fantastic resource!
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Co-Sourcing Improves Pharmaceutical Laboratory Productivity
Loss of Lives in Clinical Trials...
Celera certainly isn't acting like a stepdaughter about to be disowned, even though parent company, Applera, reported an SEC filing to spin off the firm. In fact, Celera has posted strong financial results for the last two quarters and closed two major acquisitions, which are already adding to its profitability. Celera is a diagnostics firm that offers tests and services. Besides instruments that predict disease risk and optimize therapy selection, Celera also leverages its discoveries and pharmacogenomic tests in therapeutic development partnerships.Year after year, though, the company seems to be refocusing its franchise on diagnostic. This includes fewer proteomics-based research activities and more company buy-outs to pad the diagnostic segment. Celera's standing is also improving in the eyes of investors and analyst. While the acquisitions are recent and some are holding out to see evidence of full integration, the immediate effect of creating a profitable company is definitely being lauded."We view Celera's plan to shift the focus to its molecular diagnostics business as positive to the company," says Grant Zeng, Zacks senior pharmaceutical industry analyst. "The diagnostics market will enjoy a high growth in the next few years. Also, personalized medicine has gained favor in the past few years, and the trend will continue in the future." Zeng finds that the decreased focus in drug R&D is also crucial to the firm's success. Bruce Cranna, senior analyst at Leerink Swann, echoes this sentiment. He has an Outperform rating on the company and believes that the possible spin-off from Applera, expected to go through midyear, will work to its advantage. Celera will have better access to capital and will be able to attract more investors such as those who weren't able to own tracking stock, he states.While Zeng certainly sees the advantages of being an independent company, he has one trepidation. "The separation may hurt the cooperation with sister company, Applied Biosystems, or increase the cost of cooperation." For Zeng, Celera's stock is labeled a Hold. In addition to the caution related to the spinout, he identifies other pitfalls related to its recent acquisitions. In October 2007, Celera closed two takeovers: Atria Genetics for $33M and Berkeley HeartLab (BHL) for $195M. These added to Atria's line of human leukocyte antigen testing products and BHL's portfolio of CLIA-certified cardiovascular tests and disease-management services. "We would like to see evidence of the company's ability to successfully integrate and leverage the commercial operations of Berkeley and Atria," notes Zeng. Cranna, however, finds that the acquisition of BHL gives Celera an interesting hybrid business model. BHL provides testing services, while Celera is developing the actual cardiovascular diagnostics. Though this could slow down the overall growth rate, BHL will add significant product sales, he points out. Celera's last financial statement is evidence of this fact. Revenues in Q2 saw an approximate 150% jump over Q1 to $40.3M. The acquired companies alone contributed $23.6M. Celera expects to be profitable on a non-GAAP basis in the second half."We anticipate an interesting growth story in the next one to five years," comments Cranna. "We're expecting to see about a 20% sales growth." He points out that the FDA-approved m2000 technology shows potential and is still in the early phases of commercialization. The company is not completely free from risks, Cranna notes. In terms of its marketed products, competition is the issue. "Celera is essentially going head to head with Roche." Secondly, "the risk with its pipeline products will grow if the regulatory landscape changes such that FDA gets more involved in pharmacogenomic testing." As of December 31, 2007, Celera's cash and short-term investments were approximately $342M. Cranna sets his price-target range between $18 and $20, which he expects in the next 12 months. Zeng's target is slightly lower at $16. The company opened trading on March 27 at $14.62.
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MDMA (commonly known as the empathogen "ecstasy") is classified as a Schedule 1 drug, which is reserved for compounds with no accepted medical use and a high abuse potential. Two researchers from Stanford, however, call for a rigorous scientific exploration of MDMA's effects to identify precisely how the drug works, the data from which could be used to develop therapeutic compounds.Do you agree that ecstasy should be studied for its potential therapeutic benefits?
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text: Console Gaming Is Dead (In This Simpsons Gag)Jason Schreier10/03/13 4:00pmFiled to: the simpsons: tapped outsimpsonsthe simpsonseakotakumobile671EditInvite manuallyPromoteDismissUndismissHideShare to KinjaToggle Conversation toolsGo to permalink Today's Halloween-themed update to The Simpsons: Tapped Out has a new loading screen filled with some excellent gags, including a special shout-out to console gaming. If you can't read the screenshot up there, here's a higher-res version (click expand): Particularly poignant when you consider how many kazillions of dollars Simpsons: Tapped Out is bringing in for EA every day. Advertisement (Thanks, Nick!)Reply67 repliesLeave a reply


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What are you looking for?
Visitors & Travel Insurance 101
Learn about travel medical insurance that provides health, injury, and travel emergency coverage while traveling or during a stay abroad.
How does visitor insurance work?
Learn how you can use your insurance plan in case you have a medical situation, like sickness or injury.
How much will insurance pay?
Estimate and compare how much each visitor insurance policy will pay for covered eligible expenses.
Help me choose an insurance plan
Find the most suitable insurance plan for you need, by just answering a few questions.
What is Policy Maximum & Why is it so Important?
The video also helps you explain how much policy maximum you should choose or select for your visitors insurance.
Policy Maximum is the limit of money that the insurance company is promising to pay for your covered benefits. It is important to select the right policy maximum while purchasing a travel insurance as it affects your risk and liability. It is recommended to buy a travel medical insurance with higher policy maximum as it equates to better protection and less liability.
What is a Deductible & How Does It Affect Travel Insurance Cost?
The video will help you understand the term "Deductible", Why is Deductible so important and how does deductible affect your travel insurance cost or premium? It will also show case how much Deductible should you select while buying a travel medical insurance.
Deductible is the amount of money you are responsible for, before the insurance company starts paying for any covered expenses.
There are two types of deductible
1) Deductible per incident
2) Annual Deductible or Deductible per policy period
Understanding Limited Coverage Plans & How Do They Work?
Limited Coverage insurance policies are basic travel medical insurance plans that provide pre-defined fixed dollar amount for each medical service. The video also explains an example as to how does a limited coverage plans work.
Limited coverage plan may not provide adequate coverage should you face a serious medical coverage. It is important to go through policy's Description of Coverage to understand policy limitations.
What are Comprehensive Coverage Plans & Why Should They Be Preferred?
The video helps you understand the concept of comprehensive travel insurance plans and why they are preferred over other insurance plans. One of the reasons is that comprehensive travel medical insurance plans provide better coverage and higher benefits. They may cost more, but they provide better coverage and more protection. Most travel medical insurance policies with comprehensive coverage, provide percentage-based coverage with lower out-of-pocket expenses. This video showcases an example of a comprehensive coverage travel insurance plan and explains how they work.
Understanding Coverage For Pre-Existing Medical Conditions
This video describes why pre-existing medical conditions are not covered by most travel medical insurance plans, and why purchasing travel insurance is still a wise choice to make for your international trip to the USA.
Understanding Accidental Death And Dismemberment (AD&D) Coverage
It is important to provide the beneficiary name, as it is the person who will receive the insurance benefit in case of death of the insurance.
Accidental Death and Dismemberment (AD&D) Coverage provides protection if your death is the result of an accident. It also provides benefits should an accident cause the loss of hands, feet, eyesight, speech or hearing.
What is Common Carrier Accidental Death Coverage?
Understanding Common Carrier Accidental Death - Accidental Death and Dismemberment Common Carrier Coverage provides benefit amount to the beneficiary named on the policyholder's application in the event of travelers death due to an accident while on a fare-paying common carrier or public transportation like commercial airplane, train, public bus etc. It does not include personal cars, rental cars, chartered planes, or personal boats.
What is Return of Mortal Remains or Repatriation Coverage?
'Return of Mortal Remains' or Repatriation benefit is a coverage provided to send the travelers' mortal remains to their home country in the event of death on a foreign land.
Repatriation coverage provides the cost of transporting the deceased body back to the home country. The travel insurance assistance department will make necessary arrangements including negotiation with customs, airlines arrangements, handle shipping cost etc., provide guidance and co-ordinate to transport the deceased body back to the home country.
Travel Medical Insurance: Deductible Explained
Understanding what is travel medical insurance policy deductible and why is it so important? It is crucial to understand important terms when referring to travel medical insurance to chose the most appropriate policy for your needs. This video will hopefully give you clear understanding of deductible and how deductible affects your travel medical insurance cost. You should select the deductible accordingly based on each individual policy.
What is Annual Deductible or Deductible Per Policy Period?
This video will help you understand what is annual deductible or deductible per policy period and why it is important while shopping for a travel medical insurance policy. Insurance policies where the deductible is paid annually or per policy period, require that you pay the deductible once a year before insurance begins paying for covered benefits.
Learn information on what deductible to choose from, where deductible is paid annually or per policy period. In order to keep in the insurance cost low, you can select a high deductible but if you would like to keep your liability or out-of-pocket expenses low, choose a low deductible
What is Deductible Per Incident & How Does It Work?
Here is a video that will explain and help you understand how deductible per incident works. For travel medical insurance policies where the deductible is required per incident, you are required to fulfill the deductible for each incident that occurs while you are covered by your policy in order for the insurance to begin paying for covered benefits. Additionally, this video will assist you in making the decision for how much deductible you should choose when going with a deductible per incident policy.
What is Emergency Medical Evacuation & Why is it so Important?
This video will help viewers understand what emergency medical evacuation is, and why it is so important when choosing a travel medical insurance policy. Emergency medical evacuation offers coverage for expenses necessary for transporting a seriously injured or ill person to the nearest medical facility. It also includes the cost of transportation to get you to the nearest medical facility. Simply put, this insurance protects you in case of a medical emergency that requires evacuation during your trip.
What are Usual, Customary and Reasonable Charges or UCR Charges?
Usual, Customary and Reasonable Charges also referred to as UCR - is an established maximum amount that an insurance company will reimburse for a specific service or procedure covered under your insurance policy. These charges are generally determined based on charges that are actually billed by providers for each procedure or service in a geographical area.
What is Hazardous Sports or Adventure Sports Coverage & Who Needs It
Hazardous sports or Adventure sports are any activities that are deemed risky because risk equates to higher-than-average rates of injury and/or death. It is important for travelers who may be participating in such adventurous sports or activities that are considered more risky or have health hazards, to have a proper adventure sports travel insurance coverage.
Activities such as motorcycling, scuba diving, jetski, parasailing, skiing, mountain climbing, sky diving, race car driving, bungee jumping and even surfing are some examples of hazardous activities.
See More Educational Videos
Top Common Questions
- What is Visitor Insurance and How Does It Work?
- How to Pick The Right Visitor Health Insurance Policy?
- Which Doctor Can You Go To?
- How to Use Visitors Insurance at Pharmacy?
- How Reliable is the Insurance Company?
- Visitor Insurance Claim process?
- Why VisitorsCoverage?
Tips for Buyers
- Comprehensive vs Limited plans - Key differences
- Which Visitors Insurance to choose?
- Best Visitor Insurance for USA
- Visitor Insurance from India or USA
- Travel insurance USA-Top 5 features to look for
- Visitor Insurance Purchase Process
- How to use your Visitor insurance
- Keep your Travel health insurance cost low
- Visitor and Travel insurance Terminologies
- Why buy a USA visitor insurance policy
Travel Insurance for Seniors
- How to Pick the Best Travel Insurance for Seniors
- Travel Insurance for Visitors age between 70 and 80 years
- Travel Insurance for Visitors age 80 years or over
Insurance for Green Card holders
- Health Insurance Plans for GreenCard & New Immigrants
- Social Security Benefits for Green Card Holders
- Medicare for Green Card Holders
- Green Card Health Insurance
- What is Medicare?
- Medical Insurance for GreenCard Parents
- Medicare vs Medicaid
Insurance for Pre-existing Conditions
- Acute Onset of Pre-Existing Conditions
- FAQs: Acute Onset of Pre Existing Conditions
- Pre Existing Conditions for Travel Insurance
- Pre Existing Conditions & other Non-Covered Benefits
- Pre-existing Conditions for Visitor Insurance
- Pre-existing Conditions Plans for USA Visitors
- Pre-existing Conditions : Unpaid Bills
Short-Term Health Insurance
- Short-Term Health Insurance
- Short-Term Health Insurance FAQs
- Short-Term Vs. Regular Health Insurance
Pregnancy Coverage
Travel Insurance
- International Health Insurance
- Medical Tourism Insurance
- Insurance for visitors to Canada
- Travel Insurance for US citizens visiting USA
- Travel Insurance for US Citizens and Residents
- Travel Insurance for Schengen Visa
- Missionary Insurance
- Trip Insurance
- Group Travel Insurance
- Business Travel Insurance
- Trip Cancellation Insurance
- International Travel Insurance
- International Trip Cancellation Insurance
- Cancel for Any Reason Insurance
- Cruise Insurance
- Kidnap & Ransom Insurance
- Mountaineering and Hiking Insurance
- Flight accident insurance
- Medical evacuation Insurance
- Travel Insurance for OPT Students
- AD&D Insurance
- Trip delay insurance
- Multi trip insurance
- Rental car collision insurance
- Travel Accident Insurance
- International Life Insurance
- Pilots Disability Insurance
- Sports Team Group Insurance
Expatriate Health Insurance
Domestic vs Visitors Insurance Plans
ObamaCare (Affordable Care Act)
- The Basics of Obamacare
- ObamaCare for Visitors to USA
- Obama Care for Green Card Holders
- Obamacare for US visa workers
- State-Wise Health Insurance Marketplace


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CHICAGO-Ventas Inc. capped a year of accretive investment with its best-ever showing for funds from operations, the healthcare REIT reported Friday. Normalized FFO reached $4.14 per diluted common share, or $1.2 billion in total, for the 12 months that ended Dec. 31, 2013, a 9% gain year over year.
Debra A. Cafaro, chairman and CEO of Ventas, calls '13 "another outstanding year," one that also saw the company drive cash flows from operations to a new high of $1.2 billion, up 20% Y-O-Y. Further, Ventas last year increased its dividends by 10%, during a period that saw the company "maintaining financial strength and flexibility and executing our strategy consistently," adds Cafaro.
The REIT attributes its performance to $1.8 billion of investments last year, full-year results from its 2012 acquisitions, strong same-store growth in the 235 seniors housing properties managed by Atria Senior Living and Sunrise Senior Living, rental increases from its triple-net lease portfolio and lower weighted average interest rates. Ventas added two more seniors communities to its portfolio in Q4.
Looking ahead, Ventas on Friday projected 2014 normalized FFO per diluted share, excluding the impact of unannounced acquisitions, divestitures and capital transactions, of between $4.31 and $4.37. That guidance range represents growth of approximately 5.5% to 7% per share.
Ventas in Q4 also lined up a new $3-billion unsecured credit facility, with a balloon feature that allows it to expand its borrowing to $3.5 billion. For more insight into the company's success and how Cafaro has approached her leadership role as she built the company into one of the REIT world's biggest players, check out this Real Estate Forum profile of the CEO and her mentor, Shelli Rosenberg.


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Although a decision has not been made, members of the Glendale-River Hills School Board on Wednesday said they likely would not be interested in participating in a consolidation study with Nicolet High School and fellow K-8 feeder districts Fox Point-Bayside and Maple Dale-Indian Hill if monetary contributions had to be made.
Current budgetary constraints were cited as a reason.
School Perceptions, a consulting firm hired by Nicolet, issued a survey to households in Bayside, Fox Point, Glendale and River Hills in December. Nearly 80 percent of the more than 1,100 respondents favored exploring consolidation if long-term cost savings could be realized.
Administrators in all four districts will continue to discuss the issue at shared services meetings. The next one has been scheduled in May.
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- University School was on "soft lockdown" after police shooting in Milwaukee
- North Shore Police Reports: June 18, 2015
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- University School of Milwaukee students aim to provide 60 mattresses to homeless shelter


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Ought a be very interesting.
Sentiment: Strong Sell
I am thinking about filing a class action suit (or get one started) against the organic food making industry that is conspiring to destroy my investment in Monsanto by sabotaging the wheat harvest crop(likely) and then calling it 'the doom that ends all doom' or similar words, and creating undue fear before anything was done and decided upon, and making false accusations before any proof of wrongdoing against Monsanto, and include the suit against half the MotleyFu writers for having a supporting role in propagating a 'one view only' again complete false or misleading view against the role of Monsanto, and sending people(likely from the organic food industry to various message board forums, including this one, in order to command the interests of and force their views upon us, attempting to sway our investing opinions, and our eating choice opinions too. Even if I did not win, maybe I can at least find out the truth of who is behind all of this overwhelming negativity, spite, and often downright lies.
Sound like a winner?!?
sounds like your an idiot .... what is behind negativity towards monsanto is called common sense .... you may not understand .... so sorry
go for it dude, let us how that works out for you
hey, they are selling, the big boys are selling, keep pumpin. its embarrassing to watch you lower yourself to such an untennable position, just google monsanto, its not pretty bro.


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With the rising economic importance of human resources and skills, employment and training agencies are often expected to play a more important role in local strategies to support new job creation, facilitate restructuring and increase productivity. The OECD Local Economic and Employment Development (LEED) Programme has developed a series of reviews on Local Job Creation to examine the contribution of local labour market policy to boosting quality employment and enhancing productivity. For Poland, the review has looked at the range of institutions and bodies involved in employment and skills policies, focusing on local strategies in the city of Poznań and the Radomski sub-region.
This database provides information on environmentally related taxes, fees and charges, tradable permit systems, deposit refund systems, environmentally motivated subsidies and voluntary approaches used in environmental policy in OECD member countries and a number of other countries. Developed in co-operation between the OECD and the European Environment Agency.
Data on government support to agriculture in the OECD area and other major economies, measured by the Producer Support Estimate (PSE) and Consumer Support Estimate.
English, PDF, 1,193kb
This note presents selected findings based on the set of well-being indicators published in How's Life? 2016.
These ready-made tables and charts provide for snapshot of aid (Official Development Assistance) for all DAC Members as well as recipient countries and territories. Summary reports by regions (Africa, America, Asia, Europe, Oceania) and the world are also available.
English, PDF, 175kb
Poland is ranked 21st among the 34 OECD member countries in decreasing order with a tax wedge for an average single worker at 34.7% in 2015, compared with the OECD average of 35.9%. The country occupied the same position in 2014.
The 2015 edition of National Accounts of OECD Countries, General Government Accounts is an annual publication, dedicated to government finance which is based on the System of National Accounts 2008 (SNA 2008) for all countries except Chile, Japan, Korea and Turkey (SNA 1993). It includes tables showing government aggregates and balances for the production, income and financial accounts as well as detailed tax and social contribution receipts and a breakdown of expenditure of general government by function, according to the harmonised international classification, COFOG. These detailed accounts are available for the general government sector. Data also cover the following sub-sectors, according to availability: central government, state government, local government and social security funds.
The data in this publication are also available on line via www.oecd-ilibrary.org under the title OECD National Accounts Statistics, General Government Accounts (http://dx.doi.org/10.1787/na-gga-data-en and http://dx.doi.org/10.1787/na-gga08-data-en).
Growth has been robust in Poland, but reforms are needed to sustain improvements in living standards. Developing skills and infrastructure, strengthening the labour market, public finance and green taxes, would raise growth and well-being.
Polish economic growth remains solid and unemployment is decreasing, but further investments in infrastructure and skills will be essential to sustain a continuing improvement in living standards, environmental quality and well-being, according to the latest OECD Economic Survey of Poland.
The State continues to remain an important shareholder in listed companies worldwide, especially among emerging economies, which rely increasingly on mixed-ownership models. With the benefit of hindsight and more recent examples, this book provides fresh perspectives on the motivation to list state-owned enterprises (SOEs) and the process it entails. Drawing from the experiences of five economies (People's Republic of China, India, New Zealand, Poland and Turkey), the book concludes that broadened ownership generally has a positive impact on the governance and performance of these companies. However, country practices show that the act of listing cannot guarantee that these companies are completely averse to State interests; and deviations from sound corporate governance practices, as enshrined in the OECD Guidelines on Corporate Governance of SOEs, can in some cases, raise concerns with regards to non-State shareholder rights, commercial orientation, board independence, conflicting State objectives, transparency, disclosure and more.


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Miley Cyrus On Jimmy Kimmel Live Practically Naked!
Jimmy Kimmel was joined by Miley Cyrus Wednesday August 26th. Her outfit that she wore, however, is what everybody is talking about!
Jimmy Kimmel was joined by Miley Cyrus Wednesday August 26th. Her outfit that she wore, however, is what everybody is talking about!
Who can possibly follow NeNe Leakes on 'RHOA'? A beautiful transgender model with a mermaid obsession, that's who.
Pratt says she used to be "so excited to take [her] meth" in her new memoir.
Aspiring filmmakers. Androids. Cheerleaders. Olympic figure skaters. Dr. Frank N. Furter. Moses.
The last 12 acts of America's Got Talent have now performed, and the votes are in. Here are your 8 acts moving on to the semifinals.
We're closer than ever to Stephen Colbert's Late Show debut on September 8, and while George Clooney's guest appearance gave a marquee head start, the full guest list for Colbert's full week offers a much clearer view of the new series. Everyone from Jeb Bush to Elon Musk and Amy Schumer will fill out the ranks, with musical guests on deck as well.
The last 12 acts of America's Got Talent performed for their chance to move on to the semifinals. Here are the top acts from last night's show - the acts that deserve to advance in the competition.
American Horror Story: Hotel had nothing to show by its presence at Comic-Con 2015, but with filming in full swing, Season 5 is dripping an elevator's worth of fresh blood. Not only do we have our first photos of the cast, but also fresh details on a "drillbit dildo" killer, connections to the first season, and much more.
Have you ever been to the Columbus Baking Company in Syracuse? If you haven't you can see what they're all about in a new PBS special airing tonight, August 25th. It's called 'A Few Good Bakeries' and it will be shown at 8 p.m.
Most people haven't even heard of Gouverneur, New York never mind know where it is. Thanks to Steve Moses, the small upstate town is getting national attention. The college student is competing on Big Brother and he's become a local celebrity.
Comedy Central took a bit of a breather after Jon Stewart's final Daily Show to let Stephen Colbert soak up some spotlight at CBS, but with Trevor Noah's September 28 premiere fast arriving, it's time to put asses in the chairs. Groove to some Kanye and lower your seat for our first look at The Daily Show With Trevor Noah.
Ever wonder how litigants end up on the 'Judge Judy' show? An upstate NY man recently shared a letter he got from the show's producers on reddit.


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The Hollywood sign (and a car interrupting this photographer) in Hollywood.
The backside of Paramount Studio in Hollywood, as seen from the parking lot of Astro ...
Scenic view reflected off the Kaiser building in Kearny Mesa
Sushi chef preparing my dinner at Todo's Sushi in Scripps Ranch
Ski Lifts At Snow Summit
Collection of Mailboxes in Ramona
Horse path at Canyonside Stables in Rancho Penasquitos
A view of the valley at the end of Poway Road, in Poway.
A very busy Regina's Pizza in the North End, Boston MA
three friends stroll around Lake Miramar.
Cyclists take to the steets in Rancho Penasquitos.
Sign above the Ramona Theater in Ramona, CA
Webb lake Park in Rancho Bernardo
Webb Lake Park in Rancho Bernardo
Lake Miramar on an overcast morning
Winetasters at Danza Del Sol Winery in Temecula
Baseball field at Canyonside Park in Rancho Penasquitos
Pamo Valley Winery is waiting for you in Ramona
Wine tasters at Danza Del Sol winery in Temecula.
Rows of boots waiting to be rented at the snowboard rental shop in Big Bear.
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Wally Hall is the managing sports editor for the Arkansas Democrat-Gazette. A graduate of the University of Arkansas-Little Rock after an honorable discharge from the U.S. Air Force, he is a past president and member of the Football Writers Association of America, member of the U.S. Basketball Writers Association, past president and current executive committee and board member of the Arkansas Sports Hall of Fame, and voter for the Heisman Trophy.
Players, fans pump life back into Walton
LITTLE ROCK It could have been almost any basketball Saturday afternoon in the 1990s.
Bud Walton was rocking, the Arkansas Razorbacks were rolling.
It was Razorbacks basketball before Stan Heath and John Pelphrey.
It was a rebirth of Hawgball — that perspired-inspired pressure defense that turns strong legs into jelly pegs and makes shot decisions come from the belly instead of the brain.
Michigan could have watched a decade's worth of game tapes and still not had a clue what it was about to endure: Hawgball feeding off a picc line that ran directly into the hearts of the Hogs faithful who remembered how and when to rally their team. And when the Wolverines' final three-point attempt missed the Razorbacks had notched a quality victory, 66-64.
It was the first sellout crowd since March 1, 2009, when the 1994 national championship team was honored. Athletic Director Jeff Long, who had the idea of bringing back Nolan Richardson and his troops, knew what the fans expected.
What they wanted and needed.
It wasn't just a style, system or set of players.
It was a coach and team whose every fiber understood the pride and passion of wearing the Razorbacks uniform. Something the fans totally respect.
A desire that started with Eddie Sutton in the 1970s and swelled under Richardson that could not, would not be satisfied with words or explanations.
What the fan base expected, wanted and needed was simple: discipline, desire and all-out effort.
Like taking charges and diving on the floor for loose balls, two things that aren't just taught but instilled by example.
Sutton and Richardson, right or wrong, always had intense passion.
So finally, nearly 10 years after being dismissed when he asked to be paid off, Richardson was in the house watching the young man he mentored.
Mike Anderson didn't disappoint. His charges, young in age, came out firing jabs, hard rights and uppercuts as they went after the Wolverines as if it were the NCAA championship game.
Michigan wasn't ready for those first 10 minutes when the game was won.
It couldn't be. It has been more than a decade since so many fans showed up at Walton Arena with one goal, to be the sixth man.
As the crowd roared, the Razorbacks fought to a 30-10 lead, making their first 11 field goals. That was a pace no team can maintain for 40 minutes, but that wasn't what would win this game.
In those opening 10 minutes the Hogs controlled the tempo and dominated the game. When Michigan slowed it down and closed within 46-33 at the half, it wasn't really the Razorbacks defense.
What allowed Michigan to get within a field goal at the end was rebounding. The Wolverines got far too many second-chance points.
A flagrant, almost dirty, foul by Michigan's Zack Novak on BJ Young, who has become the go-to guy, fired up the crowd and the Razorbacks moved back in front 61-48.
Michigan nailed some big threes, the Hogs missed some key free throws and with 21.2 seconds to play, Ky Madden made the play of the game.
He refused to give up on a missed free throw and stole the rebound between two Michigan players.
It resulted in another missed free throw, but it helped get enough time off the clock that Michigan had one shot left and every dribble was contested, forcing a long three by Trey Burke that was just off the mark.
The signs of a rebirth of Hawgball were everywhere.
Sports, Pages 23 on 01/22/2012


text: AdChoices Terms of Use Privacy Policy Hide TOP 10 Top 10 Better Man Only Watch Share Tweet 0 Shares 3. The Gerald Genta Fantasy Racing for Only Watch Only Watch Corny? Perhaps. But the fact is that kids love Mickey Mouse, and boys will love a racing Mickey Mouse for sure. Genta's retrograde watch features a Mickey Mouse in a racing outfit, a checkered flag dial out of carbon, and the back of the watch has the Formula 1 Grand Prix Monaco circuit engraved. This watch was auctioned during the Only Watch of 2005 and fetched €16,000. Next Page TOP 10 9 8 7 6 5 4 3 2 1 Comments Share your opinion Your name More Like This Watch Snob® The Watch Snob: Is It Classy To Have A Giant Logo? Read More Watch Snob® The Watch Snob On BaselWorld Read More Watches The World's Most Popular Perpetual Calendars Read More Watches The Most Sought-After Complication Read More Watch Snob® Watch Snob: The Best Heirloom Watches Out There Read More Watch Snob® Watch Snob: Is There A Rule For How Much To Spend? Read More Around the Web Special Features Guy Gear The Best Watches Under $500 Read More Special Feature The Ultimate Guide To Hooking Up Online Read More


text:
The Ben Heck Show's Felix hacks a guitar
And now for something completely different.
Ben Heck's soldered Atari 2600
Last week it was a giant Game Boy. This week he's moving on to Atari.
Ben Heck's giant Game Boy
It is what it sounds like.
Ben Heck's FPGA dev board tutorial
This one's for the newbies.
Ben Heck's Raspberry Pi Bitscope mod
Fun with Autodesk Fusion 360.
Ben Heck's Steam controller-inspired concept
This DIY project takes some clear cues from Valve.
Ben Heck's DIY Novena laptop, part 3
Ben and team have some problems left over from part two that they need to address.
Ben Heck's Atari Video Music teardown
Ben goes beyond retro gaming machines with the "Atari Video Music," uncovering customized microc...
Ben Heck's DIY Novena laptop, part 2
Fun with open-source notebooks!


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Located near the Marais (Lappe Terrasse)
Size56 m²
Floor No.(with elevator)
- Family/child friendly
- Kitchen
- Washer/dryer
- Elevator in building
- Balcony/terrace
|Rue de Lappe, 75011 Paris, France
|Property type
|Property ID
|Acceptance rate
|Response time
|A few hours
|Calendar updated
This large apartment is located in the heart of Paris. It is located in the district of Bastille and within 5 minutes walk from the Marais district. The access to major tourist attractions is very fast and very simple. The nearest metro station is Bastille, in less than 10 minutes you are in the Louvre or Notre Dame, in 20 minutes at the Champs-Elysees. The access from the Gare du Nord and Lyon as well as Roissy CDG and Orly is very simple. There are many shops, supermarkets, restaurants and cafes at the foot of the building. For several days or more, enjoy a Parisian apartment, situated in a typical building of the Bastille district (with access through two security doors), a living-room with natural light, a separate kitchen, with a large separate bedroom and a bathroom. There is also a 13m ² terrace to enjoy the sunny days. The apartment is fully equipped.
Please notice that the flat is "ready to live in".
The flat is very bright and covers a space of 56 sqm with a living room, a separated kitchen, a separated bedroom overlooking the enlivened street but calm inside (double windows and blinds), a bathroom and a toilet. And a terrace of 13m² (to be furnished soon).
The place is fully furnished included CD player, TV. The kitchen is fully equiped. Kitchenware are provided. There is one large bed 140 x 200cm, a double sofa bed 140 x 200cm and a single sofa bed for a fifth person. A baby cot could be added (10 Euros per day).
The additional fees for this apartment are:
- A service pack of 45 € (including one set of linen, one cleaning on arrival and some cleaning supplies).
- An insurance of 11 € (see terms and conditions).
- An occupancy tax of 0.83 € per night and per adult from 18 years old, requires by the Mayor of Paris.
The rate does not include bank transfer fees (usually there is no fee inside Euro countries for regular bank transfer) nor Paypal fees.
|Price for > 4 guests
|$ 12 US per guest/night extra
|Check-in after
|Check-in before
|On arrangement
|Check-out before
|Cancellation Policy
Everything would be OK except the fact that someone has enetered our flat while we were sightseeing and has left us a message that they also have booked this flat for same days. Don`t know whos fault is it but it shouldn't happened.
stay really bad, I will not go into details but absolutely bad experience in every sense, I confirm the negative things that have been said by others stumbled badly.
soggiorno davvero negativo, non voglio entrare nei particolari ma assolutamente brutta esperienza in tutti i sensi,confermo le cose negative già dette dagli altri mal capitati.
The apartment is very centrally located in the Bastille district, bike / metro you quickly to all the important places! It is clean and functional, but the room is the Rue de Lappe, not for the suitable sleep, as it is very, very loud, and the shutter was broken at the time of our visit.
Die Wohnung liegt sehr zentral, mitten im Bastille-Viertel, mit dem Fahrrad/Metro ist man schnell an allen wichtigen Orten!!! Sie ist sauber und zweckmäßig eingerichtet, allerdings ist das Zimmer zur Rue de Lappe hin, nicht zum Schlafen geeignet, da es sehr ,sehr laut ist und der Rollladen zum Zeitpunkt unseres Besuches defekt war.
This apartment was filthy and disgusting! All of the furniture was worn and smelly. Every inch of fixtures, walls, windows and, blankets were dirty and neglected. It looked like it hasn't been cleaned since 2009 when the pictures on this website were taken. Upon arrival my husband was so depressed but without another option of a place to stay we spent 3 hours cleaning this place because we knew we would not get our money back. There is no place to dump your trash although they inform you that it is on floor -2 there is no access by elevator to that floor. You are not renting this place from an owner but from a poorly run management company called centreparis.com. Rather than update their own website which only lists their properties in french they have decided to list their properties through a more reputable company like wimdu allowing you to believe you are renting from the actual owners. There is a total lack of service regarding responding to customer concerns both before and during the rental period. There is a strict cancellation policy so once they have your money you cannot get a full refund. Basically they are renting this apartment for more than they can get in a monthly rental but are doing nothing to keep up the place. Upon arrival in Paris you must go to the Centre Paris office in another part of town to collect the keys. The only thing they provide you with is clean linen but the towels smelled like cigarette smoke. Rather than spend my precious vacation time argueing with this rental company we cleaned the apartment from top to bottom and washed our blankets and linen at the Laverie located on Rue de Lappe nearest to Rue de la Roquette. They are open from 0700-2200. We had the apartment manager enter the apartment twice during our stay telling us they were there to get estimates to cut the trees on the terrace and to fix the blinds in the bedroom. Really?! Repair something? How about cleaning the place?! This rental is located on Rue de Lappe which has always been known as Paris' nightclub central. You will have the noise of people partying till all hours of the morning and the bedroom window overlooks this street. We moved the bed into the living room and the sofa into the bedroom to lessen the noise from the surrounding nightclubs. Overall, I would not recommend this rental but would just spend the extra money to find a more decent and well kept apartment. Rent this apartment if you're used to living in a dog house or are willing to do the work to clean it during your precious vacation time either way you are on your own. Do more research and google Rue de Lappe then you will know what I mean about the noise. Buyer Beware!
Hello, I've been in this apartment with my wife and little girls (5 and 1.5 years) and we were very pleased. the apartment is spacious and well divided sleeping and living area, a few minor problems (missing TV decoder, a few towels and shower curtain missing) were quickly resolved (the morning after our arrival). The room with a window on rue Lappe (shutter broken, but fortunately "closed" manually ..) is really noisy .. noise characteristic of the "festive" way ... fortunately, having stayed from Monday to Friday, we have suffered much the nightlife of this area .... I would still recommend this apartment!! hello soon, Maximum
Ciao, sono stato in questo appartamento con mia moglie e le nostre bimbe (5 e 1,5 anni) e ci siamo trovati molto bene. l'appartamento è spazioso e ben diviso zona notte e zona giorno; alcuni piccoli inconvenienti (tv mancante di decoder, pochi asciugamani e tenda doccia mancante) sono stati presto risolti (la mattina successiva al nostro arrivo). La camera con finestra su rue Lappe (tapparella rotta, ma fortunatamente "chiudibile" manualmente..) è effettivamente rumorosa..rumore caratteristico della "festosa" via...fortunatamente, avendo soggiornato dal lunedì al venerdì, non abbiamo subito più di tanto la movida di questa zona.... consiglio comunque questo appartamento !!!!!! ciao a presto, Massimo
the only positive thing we can say is that it actually lies close to the Marais and spacious. The rest was a disappointment g. First you need your key to a desk to pick somewhere else in town. The apartment is in a very noisy and unpleasant straat.Als at night no eye white close you've come to the right place. the apartment is maybe refurbished in 2009 but since then nothing gbeurd, it is completely agfeleefd, many things do not work and the cleanliness leaves too wensn other. Definitely not a place to recommend unless you voor50 euros per night or less with a gang can sleep, or at least trying to sleep.
het enige positieve wat we kunnen zeggen is dat het daadwerkelijk kortbij de Marais ligt en ruim is. Voor de rest was g
het een teleurstelling. Eerst moet je je sleutel bij een bureau gaan ophalen ergens anders in de stad. Het appartement ligt in een zeer luidruchtige en onaangename straat.Als je 's nachts geen oog wit dichtdoen ben je hier op het juiste adres. het appartement is misschien wel gerenoveerd in 2009 maar sindsdien is er niets meer gbeurd; het is volledig agfeleefd, vele dingen functioneren niet en de netheid laat ook te wensn overig. Zeker geen plaats om aan te raden tenzij je er voor50 euro per nacht of minder met een bende kunt slapen, of althans proberen te slapen.
the location is great, the apartment is great, but the cleanliness leaves something to be desired
la posizione è ottima, l'appartamento è grande, ma la pulizia lascia a desiderare
Spacious and bright apartment in a very central and nice area. If you want to go down for a drink or something to eat late or at night so there are many opportunities for it on the street with many bars and restaurants.
Rymlig och ljus lägenhet i ett mycket centralt och trevligt område. Vill man slinka ner för en drink eller något att äta sent eller på natten så finns många möjligheter för det på gatan som har många barer och restauranger.
Apartment well located, close parking, very nice area very lively. Well appointed with spacious feel. Insommorisation bad.
Appartement bien situé, proche d'un parking, quartier trés animé trés sympa.
Bien agencé avec impression d'espace.
Mauvaise insommorisation.
The apartment was well furnished. Unfortunately, the noise in the bedroom lasts until the early morning hours. The living room is a lot quieter, so that the two rooms should be replaced better. The sofa bed is only suitable for smaller people.
Die Wohnung war gut eingerichtet. Leider dauert der Laerm im Schlafzimmer bis in die fruehen Morgenstunden. Das Wohnzimmer ist viel ruhiger, so dass die beiden Zimmer besser getauscht werden sollten. Das Bettsofa ist nur fuer leichte Leute geeignet.
- Quality assured accommodation
- Every host is verified by telephone
- Secure payment via SSL encryption


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A majority of Conservative Party members want David Cameron to overrule Nick Clegg to ensure a tougher line on immigration, Europe and tax cuts, according to a poll for The Independent.
Cabinet popularity ratings among Tory voters: click here for graphic (243k)
The survey of almost 1,400 Tory members, conducted by the ConservativeHome website on the eve of the party conference, showed 61 per cent think the Liberal Democrats have too much influence and want the Prime Minister to fight harder for Tory policies on tax, Europe and immigration.
Only 11 per cent think the Tories have got the best deal from the Coalition. Another 28 per cent believe they have given much ground to Mr Clegg's party, but say this does not matter if the Government fixes the economy.
The findings show concern in the Conservative grassroots that Mr Cameron is making too many policy concessions. But Cameron allies fear the proposed shift to the right on issues like immigration and Europe would revive voters' fears that the Conservatives are the "nasty party".
According to the poll, there is strong hostility to extending the Coalition beyond the next election. Only 9 per cent of Tory members support a "continuing coalition" to deny Labour power for the foreseeable future. Ninety-one per cent want to govern without the Lib Dems after the next election and to enact more Tory policies on crime, immigration, human rights, Europe and tax.
The poll also reveals deep hostility among Tory activists to the Government's decision to boost spending on overseas aid to poor countries, despite imposing cuts in most other areas. Asked if the Chancellor, George Osborne, should cut spending on international development if the economy worsens, 82 per cent of Tory members agree and only 15 per cent disagree.
In these circumstances 68 per cent would also cut benefits such as the winter fuel allowance for richer pensioners, while 27 per cent oppose the idea. They also back deeper cuts to the welfare budget, by 68 to 23 per cent. But Tories do not want Mr Cameron to abandon his flagship pledge to raise the NHS budget each year. They oppose the idea by 59 to 33 per cent.
A majority wants cuts to be faster and deeper. While 41 per cent say Mr Osborne's deficit-reduction plan is exactly right, 42 per cent want more spending cuts so taxes can be reduced to boost growth and another 10 per cent want bigger cuts to avoid tax rises. ConHome also asked Conservative members to rate the performance of the Cabinet. Iain Duncan Smith, the Work and Pensions Secretary, was the most highly rated. Some 90 per cent are satisfied with how he is doing his job.
Education Secretary Michael Gove, is second, up from seventh in the same poll a year ago, after a shaky start when the Government was formed.
Also moving up since last year's Tory conference are Mr Osborne, Jeremy Hunt, the Culture Secretary; Sir George Young, the veteran Leader of the Commons and Francis Maude, the Cabinet Office Minister.
Party chairman Baroness (Sayeeda) Warsi has 57 per cent of people dissatisfied with her performance. She may be moved to a different job in a Cabinet reshuffle expected next spring.
The most dramatic fall is that of Kenneth Clarke, whose liberal sentencing reforms as Justice Secretary have upset the grassroots. He slumps from 14th to 24th spot, with 61 per cent of Tory members dissatisfied.
Also dropping down the performance table are Andrew Lansley, the Health Secretary, who has endured a bruising year over his NHS reforms, and Home Secretary Theresa May. Danny Alexander, Liberal Democrat Treasury Secretary, has jumped from 23rd to sixth place but Nick Clegg has fallen from 12th to 19th.Reuse content


text: Is Robin Thicke Sleeping w/ His Nanny??? Tweet Posted August 5th, 2014 @ 3:01pm (Getty) Robin Thicke was spotted with another woman and her child on the beach a couple days ago on a day when he was suppose to be performing in another country. Apparently he had vocal issues, but people thought he was ditching because of this woman that was with him and his child. Well it turns out she's the nanny, but she looks like a cute nanny, so it leads me to wonder...are they dating?? He says he still is trying to win Paula back but says he knows that's not likely anymore.


text:
Africa UMOJA Celebrates South African Song and Dance
Our Price
Full Price
From potent rhythms of tribal music to the intricate steps of gumboot dancing, journey through the jazz of Sophiatown to the inspirational joy of Gospel and the powerful pounding energy of Kwaito when the pulsating musical celebration of South African song and dance, Africa UMOJA: The Spirit of Togetherness, comes to the San Diego Civic Theatre. From Johannesburg to London and the United States, this energetic show has captivated audiences and wowed critics wherever it travels. With beautiful costumes & award-winning, internationally acclaimed choreography, Africa UMOJA is a loud, colorful and jubilant celebration of life that audiences from all backgrounds have found infectiously delightful and uplifting. The cast of 40 comes from a myriad of South African cultures, each having their own story to tell through various forms of dance and different styles of music.
There are currently no offers for Africa UMOJA: The Spirit of Togetherness.
Currently at San Diego Civic Theatre
Cinderella (La Cenerentola): Rossini's Beloved Opera -- Presale Offer
- Full Price:
- $63.00 - $235.00
- Our Price:
- $63.00 - $235.00
The San Diego Opera brings Rossini's delightful operatic treatment of Cinderella (La Cenerentola) to… More
Venue Details
The weather was cool. I wore jeans, nice top.star this tip starred
The weather was Cool, dry, lovely.. I wore Dressy casual. Don't get formal, but don't be a slob either. Makes San Diego look like a hick town..star this tip starred
More Information
*Parental Guidance: *Recommended for ages 6 years and older.
Runtime: Approximately 2 hours, 20 minutes (includes intermission).


text:
Jofran 6294
629-4 Manhattan Espresso Sofa/Media Table
Available Usually Ships within 24-48 Hours
Quick Specs:
- Width: 20"
- Height: 26"
Learn more »
Your personality is unique so your home should be too. That�s why Jofran, Inc. is committed to creating affordable and functional furniture with style. We recognize that shopping for furniture involves making a decision about style, function and price. That is why we offer various styles of living room tables, accent chairs, bedroom suites, dining room sets and entertainment stands. Each piece in our line captures innovative design techniques that accentuate form and enhance style. Our line is a trusted source of family furnishings with many options of furniture for any size home. From upholstered accent chairs to kitchen table sets, purchasing a piece of Jofran furniture means purchasing the perfect combination of style and value. Available at AppliancesConnection
- Three Drawers
- One Shelf
- Group Includes: Cocktail Table, End Table, Sofa Table, and Chairside Table
- For residential use only
- Width (Side to Side): 50"
- Depth (Front to Back): 20"
- Height (Top to Bottom): 26"
- Weight (lbs): 59
- Brand:Jofran
- Stock Id:252540
- Series:Manhattan
- Style:Contemporary
- Frame Material:Wood
- Top Material:Wood
- Color:Espresso
- Width:20"
- Height:26"
- Length:50"
- Weight:94
- Series:Manhattan
Technical Details
$239.99Free Shipping
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text:
Item: 305612
Halfords Semi Tailored Car Mats Black - Shape 3
How can I get it?
FREE Click & Collect
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Halfords Semi Tailored Car Mats Black - Shape 3The Halfords shape 3 Semi Tailored Car Mats boast premium tufted carpet and reinforced heel pad to protect your car interior. They come in 5 different shapes for a great fit - please check the table below for compatibility listings, then order the car mat shape indicated for your vehicle.
Click to view our Semi Tailored Car Mat Fitting Guide to find the best fit for your vehicle.
Halfords Semi Tailored Car Mats Black - Shape 3
- Reinforced heel pad
- Premium tufted velour carpet
- Secured anchor point and anti-slip backing to keep your car mats in place
- Universal after-market securing clip included to anchor your car mat
- Five shapes available for a better fit
- Set of four - one pack styles your whole car
Material: Carpet Type: Full Set Fitting: Semi Tailored Heelpad: Yes Securing Clip: Yes
Rated 1 out of 5 by 1 reviewer.Rated 1 out of 5 by Susan55 Do not fit car specified Bought these shape 3 semi fit car mats for a Hyundai i20 but they do not fit although they are recommended for this car. Had to return for refund.. Very disappointed customer. 06 March 20141-1 of 1
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My 99 toyota Rav4, with 110,000 miles, and v4 engine, has been shaking when I start the car. It feels like a little tremble. When I try to shift from park to drive it will sometimes stall. I also notice my gas mileage is decreasing. What could my problem be? Thanks
Tuesday, April 24th, 2007 AT 12:11 PM


text:
It was working just fine yesterday. Not sure if something happened on the server side or in WP, but today it is not masking my external links. I have to have the external links masked so the actual URL external link cannot be shared.
Ok, I was using the latest version of the plugin. But read today that if it wasn't working to try 2.172, which I did. It didn't work either so I updated it back to the newest version.
http://2014.leasepal.com/constitution/ is a sample page. It is private, so you need password: LPConstitution-2014 to access it. Once on the page, the "play" button links to another server. I need that URL masked. Like I said it worked yesterday. Today not.
I installed two new plugins today (Smart Cleanup Tools and Gravity Forms), but as part of my troubleshooting I deactivated them and it did not fix it.
Please advise.


text:
The Christmas is just around the corner, so I wrapped up a cute present for those who are already in the holiday spirit.
Here is the link to download the wallpaper, in 6 different resolutions:
Thanks ❤
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text: Our Verdict Balanced sound and an appealing tone make these headphones worth the price tag For Sound is detailed, dynamic and exceptionally balanced Against No inline mic for mobile phone users Reviewed on 30th January 2012 Grado is more famed for its traditional, on-ear, hi-fi headphones, but it's a canny company when it comes to portable in-ears too. The iGis produce an exceptionally balanced sound across the frequency range. There's a certain flavour to the sound, but it's a very appealing tone, which can be as exciting as it can be soothing depending on the type of track being played. Spin Florence + The Machine's No Light, No Light and detail and dynamics are handed out in equal measure. The quiet, insightful moments concentrating on the vocal contrast well when the drum kicks into life. There's no inline mic, which might put a few mobile phone users off, but they offer sufficient all-round ability to make them an excellent alternative to the Beyerdynamics - and worth the extra pennies. See all our headphone Best Buys More after the break Follow whathifi.com on Twitter Join whathifi.com on Facebook Related News Black Friday 2016: What is Black Friday? Where are the best deals? Thu, 21 Jul 2016, 11:47am Best headphone deals: in-ear, over-ear, wireless and more Mon, 18 Jul 2016, 02:47pm Wednesday Wrap: Tidal and Humax updates, Waves 3D Audio and Teufel surround sound setups Wed, 13 Jul 2016, 12:29pm The best Amazon Prime Day 2016 UK deals – live Tue, 12 Jul 2016, 04:42pm The best Amazon Prime Day headphones deals Tue, 12 Jul 2016, 09:24am Grado Headphones iGi