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|Table of Contents
|Vol. 23, No. 3, 2003
Issue release date: May – June
|Section title: Original Article: Basic Sciences
Down-Regulation of Connexin 32 Gene Expression through DNA Methylation in a Human Renal Cell Carcinoma CellHirai A.a · Yano T.b · Nishikawa K.a · Suzuki K.a · Asano R.a · Satoh H.b · Hagiwara K.b · Yamasaki H.c
aDepartment of Veterinary Pharmacology, College of Bioresource Sciences, Nihon University, Kanagawa; bDepartment of Food Science Research for Health, National Institute of Health and Nutrition, Tokyo, and cKwansei Gaskuin University School of Science and Technology, Hyogo, Japan Corresponding Author
Tomohiro Yano, PhD
Department of Food Science Research for Health
National Institute of Health and Nutrition
1-23-1 Toyama, Shinjuku-ku, Tokyo162-8636 (Japan)
Tel. +81 3 3203 8063, Fax +81 3 3205 6549, E-Mail email@example.com
Background: We have recently reported that connexin (Cx) 32 is down-regulated in a human renal cell carcinoma (RCC) cell (Caki-2 cell). Hypothesis: We postulated that the down-regulation of Cx32 gene in the RCC cell is due to hypermethylation of its promoter region. Methods: We estimated methylation status in the promoter region of Cx32 gene in the RCC cell by DNA digestion with methylation-sensitive restriction enzyme and PCR, and methylation-specific PCR (MSP). We also checked the recovery of Cx32 gene expression in the RCC cell treated with a DNA methyltranferase inhibitor, 5-Aza-2'-deoxycytidine (5-Aza-CdR). Results: Treatment with 5-Aza-CdR resulted in induction of Cx32 expression in the RCC cell. Hypermethylation of the Cx32 promoter region in the RCC cell was confirmed by DNA digestion with methylation-sensitive restriction enzyme and PCR, and MSP. Conclusion: We suggest that hypermethylation in the promoter region is a mechanism for the Cx32 gene repression in the RCC cell.
© 2003 S. Karger AG, Basel
Gap junctional intercellular communication (GJIC) has been considered the only route allowing direct transfer of small metabolites between cells to maintain homeostasis in multicellular organisms [1, 2]. The gap junction is made up of juxtaposed transmembrane hemichannels (connexons) provided by adjacent cells, and each connexon consists of six connexin (Cx) protein subunits [3]. So far, cDNAs from at least 15 different Cx molecular species have been cloned in mammals [4]. Different combinations of Cx molecular species in different tissues contribute to cell differentiation and cell growth control [5].
Several lines of evidence support the hypothesis that Cx gene acts as a tumor suppressor gene. For instance, when introduced into a human mammary carcinoma cell line, Cx26 gene conferred a reduced growth rate in cell culture, reduced tumor growth and restoration of differentiation potential in nude mice [6]. Recent studies including our reports have suggested that not all Cx molecular species are able to exert a tumor-suppressive effect on a given tumor, but rather than that there seems to be a Cx-cell type compatibility for this effect [7, 8, 9, 10]. That is, Cx exerts growth control only in tissues or cell types in which the particular Cx is naturally expressed. In a recent report, we have shown that Cx32 mainly expresses in normal human renal epithelial cell (a main progenitor cell of RCC), while the down-regulation of Cx32 occurs in a human RCC cell line, Caki-2 cell [11]. Also, we have confirmed that the same event occurs in human kidney tissue [11]. From the report, Cx32 may act as a tumor suppressor gene against the RCC cell. The mechanism underlying the repression of Cx32 in the RCC cell remains to be defined.
Of silencing mechanisms on tumor suppressor genes, aberrant promoter region methylation of CpG islands has attracted a great deal of attention as a mechanism of transcriptional inactivation. Aberrant cytosine methylation at CpG in the 5′ CpG islands has been associated with repression of transcription, and it is an important and common mechanism for silencing of tumor suppressor genes [12]. In this context, the present study was carried out to estimate if hypermethylation of DNA was responsible for the repression of Cx32 expression in the Caki-2 cell.
HK-2, a SV-40-immortalized human renal tubular cell line and a human renal carcinoma cell line Caki-2, were obtained from American Type Culture Collection (ATCC), and maintained in media and culture conditions as described in the following: both cell lines were cultured in McCoy's 5A medium (Invitrogen, Calif., USA) supplemented with 10% fetal bovine serum (FBS) and penicillin-streptomycin sulfate on standard plastic tissue culture dishes and incubated in an atmosphere of 95% air/5% CO2 at 37°C.
Total cellular RNA of each cell line was isolated using SV Total RNA Isolation System (Promega, Wisc., USA) as described in the manufacturer's manual, and cDNA was synthesized using Superscript Reverse Transcriptase (Invitrogen). Amplification of the Cx32 gene was performed using synthetic oligonucleotides (sense primer, 5′-ATGAACTGGACAGGTTTGTAC-3′; antisense primer, 5′-ATGTGTTGCTGGTGAGCCA-3′; PCR product, 301 bp). GAPDH (sense primer, 5′-GTATGTGGAAGGACTCATG-3′; antisense primer, 5′-GTTTTTCTAGACGGCACCTCA-3′; PCR product, 249 bp) was used for an internal control. The condition for amplification was carried out by an initial denaturation at 94°C for 2 min; 35 cycles of 94°C for 30 s, 56°C for 1 min, 72°C for 1 min, and a final extension for 5 min at 72°C. The PCR products were electrophoresed on 1.5% agarose gels, and stained with GelStar Nucleic Acid Gel Stain (Takara, Japan). Wide-Range DNA Ladder (Takara) was used as markers for sizing the RT-PCR products.
Caki-2 cells were treated with 5-Aza-CdR. 5 × 105 cells were seeded on a 60-mm plate and incubated for 24 h. The cells were cultured up to 14 days in complete media which contained various concentrations of 5-Aza-CdR (2.5, 5 and 10 µM), and media was changed at 3-day intervals. After that total cellular RNA was isolated to analyze Cx32 expression with RT-PCR. In order to estimate the level of Cx32 mRNA semiquantitatively, the ratio of Cx32 to GAPDH in mRNA level was calculated by densitometric analysis using Atto Image Analysis Software (Atto, Japan).
Methylation status of promoter region was determined by digestion with methylation-sensitive restriction enzyme, followed by a PCR method [13]. Genomic DNAs from each cell lines (HK2 and Caki-2) are digested with MspI and HpaII (Roche Diagnostics, Germany) restriction enzymes. Reaction, either no enzyme, or 75 units of HpaII, or 150 units of MspI was carried out at 37°C overnight. To determine the fragment of promoter region, 2 µl of each samples amplified by PCR in 25-µl reactions containing 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2, 100 µM dNTP, 0.5 units of Taq polymerase (Takara), and 50 ng of each primer (sense primer, 5′-GCTCTCTTTCCTGTCCTCTTT-3′; antisense primer 5′-AGCCCATCTTGTTCCGATA-3′; PCR product, 623 bp). PCR was performed with at first an initial denaturation at 94°C 4 min, followed with 35 cycles of 94°C for 1 min, 56°C for 1 min, and 72°C for 2 min, and a final extension for 72°C for 7 min. PCR products were analyzed as described previously. PCR products will be found at the promoter having methylated status when digested with HpaII. No products will be found at the promoter having unmethylated status when digested with HpaII. All of the samples digested with MspI will have no products.
The chemical modification of cytosine to uracil by bisulfite treatment is another method for study of DNA methylation. In this reaction, all cytosines are converted to uracil but 5′-methylcytosines are resistant to the modification and remain as cytosine. For extraction of genomic DNA from cell lines we used Gen Elute Mammalian Genomic DNA kit (SIGMA, Japan) as per manufacturer's protocol. Bisulfite reaction was carried out on genomic DNA as described in the following [14]; the bisulfite conversion reaction was carried out by incubating DNA with a 5 M bisulfite solution and 100 mM hydroquinone, pH 5.0, at 50°C for 4 h. Removal of surplus bisulfite was attained by using QIAEX II Gel Extraction Kit (Qiagen, Japan). Initially, bisulfite-treated genomic DNA was amplified using methylated-specific primers for Cx32 (Cx32M); sense primer, 5′-GGGGC̲GGGTGC̲GGC̲GAT-3′ and antisense primer, 5′-CTCCG̲CG̲CCTG̲CG̲CCC-3′, and unmethylated-specific primers for Cx32 (Cx32UM); sense primer, 5′-GGGGT̲GGGTGT̲GGT̲GAT-3′ and antisense primer, 5′-CTCCA̲CA̲CCTA̲CA̲CCCA̲A̲-3′). The PCR product was 245 bp and differences between methylated and unmethylated were underlined. KOD-Plus DNA polymerase (Toyobo, Japan) was used in this PCR reaction to effectively amplify CpG island regions. The PCR condition was carried out by an initial denaturation at 94°C for 2 min; 40 cycles of 94°C for 30 s, 68°C for 1 min according to manufacturer's manual.
As described previously, 20 µg of total protein extract from each clone was loaded onto a 10% gel for Cx32 and β-actin. β-Actin was used as an internal standard. After electrophoresis, proteins were transferred to nitrocellulose membranes. The blots were incubated with anti-Cx32 and anti-β-actin antibodies (Sigma). Each immunoreactive band was detected using the ECL system (Amersham, UK) and a cooled CCD camera-linked Cool Saver system (Atto). A 2-D densimetric evaluation of each band was performed using the above Image Analysis Software (Atto). Molecular sizing was done using Rainbow MW marker (Amersham). Protein concentrations were determined using DC Protein Assay System (BioRad, USA).
The degree of cell-cell communications was measured by the extent of Lucifer yellow dye transfer from cells to cells through the gap junctions by a simple semiquantitative scrape-loading method [15]. In brief, 0.05% Lucifer yellow and rhodamine dextran dissolved in PBS were added to the cells and scrape-loaded at room temperature using a rubber policeman. The dye solution was left on the cells for 3 min, discarded and the plate was rinsed with PBS. Control plates were set by exposing the cells under similar conditions to the dye mixture but without scraping. Under fluorescent microscopic examination, the loading cells could pick up the dye mixture and were able to transfer Lucifer yellow into the neighboring cells through functional gap junctions. On the other hand, the marker dye rhodamine dextran remained entrapped, thus labeling the primary loaded at the edge of the scraped areas.
Data were analyzed by one-way ANOVA followed by Duncan's multiple-range test. p values of 0.05 or less were considered significant.
5-Aza-CdR is an inhibitor of DNA methyltransferase, the enzyme responsible for maintaining DNA methylation patterns in newly synthesized DNA [16]. Treatment of cells with 5-Aza-CdR leads to expression reversion of the gene which is inactivated by promoter methylation. Using 5-Aza-CdR, we estimated if the re-expression of Cx32 occurred in Caki-2 cells. As shown in figure 1a, 5-Aza-CdR treatment caused the induction of Cx32 mRNA in the cells, and the expression level of Cx32 mRNA expressed as the Cx32/GAPDH ratio increased in a dose dependent manner (the value, control, 0%; 5-Az-CdR (2.5 µM), 50.2%; 5-Az-CdR (5 µM), 85.5%; 5-Az-CdR (10 µM), 100%). As well, the level of Cx32 protein in Caki-2 cells was significantly elevated by the treatment of 10 µM 5-Az-CdR, while the level in HK-2 cells (a progenitor cell of human RCC cell) was almost unchanged (fig. 1b, c). In conjugation with this event, the treatment significantly increased cell-cell communication in Caki-2 cells; on the contrary, the treatment did not affect the communication in HK-2 cells (fig. 1d). These results suggest that the silencing of Cx32 gene and deficiency in cell-cell communication in Caki-2 cells relates to DNA methylation.
In order to further clarify hypermethylation of the Cx32 gene promoter region, we used the methylation-sensitive restriction enzyme/PCR [13]and the MSP methods [14]. In the restriction enzyme/PCR analysis, we estimated methylation status of a region containing exon1 and an upstream promoter region of Cx32 with TATA box in the middle, because the entire region is an extremely GC-rich CpG island (G+C content; above 60%). Furthermore, the exon1, which has no translation of the Cx32 transcription, is extremely GC-rich and contains the normally underrepresented CpG dinucleotide at the expected frequency, indicating that the exon1 contributes to transcriptional regulation of Cx32. Then, we included the exon1 in this analysis. A 623bp DNA fragment is successfully amplified from intact DNA samples which were resulted from no digestion control or methylated DNA resisting the digestion by HpaII, a methylation sensitive enzyme. Since the analyzed promoter site contains four HpaII recognition sites, no PCR products are found in unmethylated DNA samples digested with HpaII. Also, all samples treated with MspI show no PCR products. As shown in figure 2a, the PCR product was observed in no digestion samples of HK-2 and Caki-2 cells. Caki-2 cells, which had no detectable Cx32 mRNA, were resistant to digestion by HpaII and sensitive to digestion MspI, while HK-2 cells, which expressed Cx32, were sensitive to digestion by HpaII and MspI (fig. 2b). These results suggest that the silencing of Cx32 gene expression in Caki-2 cells correlates with hypermethylation of the gene promoter region.
In MSP, genomic DNA is treated with bisulfite under conditions that convert cytosine to uracil but that do not affect 5-methyluracil, and then PCR amplification of the modified DNA is carried out with a set of primers designed to distinguish methylated and unmethylated alleles in bisulfite-modified DNA [14]. The primer sequences for Cx32 were chosen for the above promoter region of Cx32 containing CpG pairs. As shown in figure 2c, Caki-2 cell showed positive for the PCR using Cx32M primers and negative for the PCR using Cx32UM primers. On the contrary, HK-2 cells showed the opposite result for the MSP. This MSP result further supports that the silencing of Cx32 gene expression in Caki-2 cells is caused by hypermethylation of the gene promoter region.
Since Cx genes have been suggested as tumor suppressor genes, it is expected that they are deleted and/or mutated in certain cancers, similar to many other known tumor suppressor genes. However, mutations in Cx genes including Cx32 were found to be rare events in carcinogenesis [17]. Thus, the question arose that how the tumor suppressor gene lost its function in carcinogenesis if there were no mutations. It is reasonable to assume that another mechanism, such as transcriptional inactivation, is important. Methylation of cytosine residues in CpG islands of the promoter region of genes has become recognized as an alternative mechanism for inactivation of tumor suppressor genes and may in fact be the most common mechanism of gene inactivation in tumors [18]. This hypothesis was strongly supported by recent studies showing that the CpG islands in p16, RB, VHL, APC and BRCA1 genes are frequently methylated in a variety of human cancers [19]. As mentioned above, it has been known that the promoter and proximal transcribed region of the Cx32 gene is CpG rich. This prompted us to examine the possibility of hypermethylation at the promotion region as a mechanism of Cx32 gene repression in Caki-2 cells. In our present study, we demonstrated that hypermethylation of the Cx32 promoter region and silencing of the Cx32 gene occurred in Caki-2 cells. Furthermore, 5-Az-CdR treatment induced the re-expression of Cx32 mRNA and recovery of capacity of cell-cell communication in Caki-2 cells. However, the recovery levels in Caki-2 cells were significantly lower than the levels in HK-2 cells. This indicates that the hypermethylation of the promoter region in Cx32 gene is a factor to induce the repression of the gene but that 5-Az-CdR-dependent release of the hypermethylation is insufficient to bring Cx32 expression and the capacity of cell-cell communication in Caki-2 cells back to that in immortalized renal epithelial cells. 5-Az-CdR has cellular toxicities even if the treatment dose is relatively low, so the toxicity may interfere with the complete recovery of Cx32 expression and the communication capacity in Caki-2 cells.
We have already reported that silencing of the Cx32 gene frequently occurs in human RCC tissues [17]. From the report and our present data, it seems possible that the silencing of Cx32 gene mainly depends on the hypermethylation of the promoter region in human RCC tissues as well as Caki-2 cells. In order to confirm this possibility, we are trying to estimate if the hypermethylation of the promoter region in Cx32 gene relates to the silencing of the gene in human RCC tissues. We have demonstrated that the recovery of Cx32 gene expression in Caki-2 cells reduces their malignant phenotypes such as anchorage independence [17]. Thus, if we confirm that the hypermethylation-mediated down-regulation of the Cx32 gene is generally observed in human RCC tissues, attenuating the silencing of Cx32 gene via hypermethylation of its promoter region leads to establishment of a new preventive procedure against the RCC.
This study was supported by a research grant from the Japan Health Sciences Foundation (grant SH24209).
Tomohiro Yano, PhD
Department of Food Science Research for Health
National Institute of Health and Nutrition
1-23-1 Toyama, Shinjuku-ku, Tokyo162-8636 (Japan)
Tel. +81 3 3203 8063, Fax +81 3 3205 6549, E-Mail firstname.lastname@example.com
Received: November 20, 2002
Accepted: March 05, 2003
Published online: May 16, 2003
Issue release date: May – June
Number of Print Pages: 6
Number of Figures: 2
Number of Tables: 0
ISSN: 0250-8095 (Print)
eISSN: 1421-9670 (Online)
For additional information: http://www.karger.com/AJN
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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California Fire Update May 18th: Progress in San Diego
Cooler weather yesterday continued to aid firefighters as they made significant progress towards containment on the remaining six wildfires burning in San Diego County. In fact, yesterday firefighters were able to contain two more wildfires leaving four remaining active fires.
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Luxperience 2013
Tourism Australia to use Luxperience event to boost awareness of high-end travel offerings
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Within the agreement, Australia's national tourism body will become a key supporter of Luxperience 2013 and will use it to promote unique travel experiences to a global market of buyers from the Americas, Asia, Middle East and Europe.
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text:
Registered User
Join date: Nov 2009278 IQ
I'm looking to get some type of pedal to detune my guitar with out having to mess with the strings. I've seen a Morpheus drop tune pedal for $200, a Boss Harmonist pedal for $150, and a Behringer US600 Harmonist pedal for $50. I'm leaning towards the Behringer because of the price. I only want to drop the tunning a couple of steps. Does anyone have/own any of these pedals and can give me some advice?
Registered User
Join date: Aug 20083,586 IQ
They mostly sound like garbage. For $200 you could get another guitar and just keep it droptuned and actually sound good.
Registered User
Join date: May 2009108 IQ
+1, i have a digitech whammy for other purposes but when i go down a step it sounds fake and muffled
Registered User
Join date: Apr 2007507 IQ
i have the morpheus and very happy with it
Chris Schementi
the alchemist
Join date: May 2009454 IQ
I'd say the Morpheus out of those. I was really close to buying one and tested it. It sounds very good with distortion. But clean you have a little bit of a chimey sound as most of these types of pedals due, but a lot less than I've heard from others. The only reason I didn't buy it was because I needed a new delay pedal more
Test one out and see if it sounds good to you.
Test one out and see if it sounds good to you.
Practicing Quattuples
Join date: Sep 2007184 IQ
No matter what, you are absolutely going to lose the natural sound of the guitar. There are no real acceptable replacements, only cheap imitations of how it would actually sound. Either get proficient at tuning down, or buy another guitar.
Join date: May 20061,405 IQ
Morpheus is the only one that's made for downtuning. The others are harmonizers.
UG's Vox fanboy
Join date: Oct 20081,115 IQ
Save yourself the trouble and do not consider getting any pedal-related object by Behringer. Ever.
And I think I'm going to side with the guys who are saying get another guitar to drop tune on.
And I think I'm going to side with the guys who are saying get another guitar to drop tune on.
Join date: Sep 20081,313 IQ
what you get when you use a pedal to downtune is a delayed attack when you pick, sure it will sound horrible regardless but...like others have said, you could get a cheap guitar, get a decent setup on it, some thicker strings, and keep that one downtuned
Registered User
Join date: Jan 200944 IQ
Don't knock the droptune till you try it. It's Amazing
Join date: May 2010646 IQ
Don't knock the droptune till you try it. It's Amazing
In terms of what it achieves, yes.
In terms of tone, it destroys it.
If you REALLY need to buy one, get a new guitar


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Timmy is ranked on...
List of the best South Park characters, with pictures and character bios when available. With such a great cast of characters, it's tough to say who the greatest really is without everybody weighing in on the discussion. Vote for your favorite South Park characters and help make this list the ultimate source for who the best characters of the show are. South...
Listen to Erinn Hayes of Childrens Hospital discuss this list on the Ranker PodcastThese are the funniest television characters ever created, as determined by the votes and selections of Ranker users. Lots of things make up truly iconic, timelessly funny characters... the actor, the writing, the supporting cast... but in the end, it's just the character that...
Timmy videos
Timmy lamm - Timmy bollar
Timmy Time Doctor Timmy 2012 DVDRip XviD ViP3R Original 003
Timmy Time Doctor Timmy 2012 DVDRip XviD ViP3R Original 001


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Hampton Inn And Suites Folsom
Hampton Inn And Suites Folsom
Hotel Guest Rating: 8.8/10
155 Placerville Road
Folsom, CA 95630
Hotel Freebies Available*
• Save 15% on select dates show dates
• Save 10% on select dates show dates
• On Sale Now -> Save 15% on select dates show dates
• Free Breakfast show dates
• Free Internet in Room show dates
• Free Parking show dates
Welcome to the Hampton Inn And Suites Folsom
Free hot breakfasts, spacious rooms with comfy beds, free Wi-Fi, free local shuttles and an indoor pool and hot tub give Priceline guests a great stay at the non-smoking Hampton Inn and Suites Folsom.
Hotel Features
The three-story hotel has 147 non-smoking rooms with flat-panel LCD TVs, clock radios, coffeemakers, desks with ergonomic chairs and free Wi-Fi. Suites add microwaves and refrigerators. Clean and Fresh Hampton Beds offer a good night's sleep, and Hampton's On the House Breakfast (or free continental breakfast to go on weekdays) gets you ready for a day of work or play. Recreation options include a fitness room, indoor pool and hot tub. Additional perks include a business center, guest laundromat, convenience store, complimentary beverage service in the lobby and free parking. The hotel offers a free shuttle to area attractions including a golf course, casino and the Foothill Wineries.
Hotel Location
Just off Highway 51, the hotel is five miles from downtown Folsom and the iconic Folsom Prison, where the museum is a popular attraction. Outdoor recreation is 15 minutes away at Folsom Lake, Lake Natoma or the American River Bike Trail. If bargain-hunting is your recreation, head for Folsom Premium Outlets, four miles away. It's 36 miles to Sacramento International Airport.
Guest Favorites
"Large rooms" and a "great breakfast" charm Priceline guests at Hampton Inn and Suites Folsom, where in addition to the "good price," they "love having a cookie/coffee available when we came back at night."
Hampton Inn And Suites Folsom Amenities
- Air conditioning
- Air conditioning individually controlled in room
- Alarm clock
- Buffet breakfast
- Business center
- Cable television
- CNN available
- Cocktail lounge
- Complimentary buffet breakfast
- Complimentary cocktails
- Complimentary coffee in lobby
- Complimentary in-room coffee or tea
- Conference facilities
- Desk with electrical outlet
- Exercise gym
- Express check-in
- Express check-out
- Family plan
- Fire safety compliant
- Fitness center on-site
- Free high speed internet connection
- Free parking
- Hairdryer
- Heated guest rooms
- High speed internet access fee
- Indoor pool
- Interior corridors
- Iron
- Ironing board
- Jacuzzi
- Laundry/Valet service
- Lobby coffee service
- Meeting rooms
- Non-smoking
- Non-smoking rooms (generic)
- On-site business center
- On-site business center is staffed
- On-Site parking
- Parking
- Parking lot
- Pool
- Public area air conditioned
- Remote control television
- Safe deposit box
- Security
- Transportation
- Transportation services - local area
- Transportation services - local office
- Valet cleaning
- Voice mail
- Welcome drink
- Whirlpool
Hotel Information
Upon check-in photo identification and credit card is required. All special requests are subject to availability upon check-in. Special requests cannot be guaranteed and may incur additional charges. Guests are required to show a photo identification and credit card upon check-in. Please note that all Special Requests are subject to availability and additional charges may apply.
Hotel Guest Reviews of the Hampton Inn And Suites FolsomOur hotel ratings and reviews are from real hotel guests. Priceline.com ensures that its reviews are guests-only by proactively sending hotel surveys to customers after they've completed their hotel stays. In the post-stay surveys, priceline.com hotel customers are asked a number of questions about their stay and the hotel. On a scale of 1-10 with 10 being the best, the survey asks guests to rate their overall experience and specifically rate the hotel's cleanliness, staff and location. Priceline.com does not allow site visitors to post unsolicited hotel reviews.
Sort by: All Travelers | Business Traveler(3) | Solo Traveler(3) | Traveling as Couple(11) | Family with Young Children(6) | Senior Traveler(2) | Group Traveler(2)
Lilia from Peoria, AZ
July 11, 2016
July 11, 2016
Bed is comfortable. Bed sheets clean. Decent breakfast choices.
Verified Hotel Guest from Redwood City, CA
July 5, 2016
July 5, 2016
Proximity to where I was visiting family & friends.
The refrigerator in the room was noisy, and so we had to turn down the thermometer to zero in order for it to stop making the noise throughout the night.
Jonathan from Alameda, CA
June 14, 2016
Family Traveling with Young Children
June 14, 2016
Family Traveling with Young Children
One elevator didn't go to all floors
Teresa from Ellensburg, WA
June 11, 2016
Business Traveler
June 11, 2016
Business Traveler
The bed was so comfortable and clean.
Virginia from Mccloud, CA
May 16, 2016
Senior Travelers
May 16, 2016
Senior Travelers
cleanliness, polite staff, quite.
Barbara from Reno, NV
April 22, 2016
Business Traveler
April 22, 2016
Business Traveler
Comfortable bed. The staff was so helpful. Needed best way directions and they supplied. Breakfast was great
Verified Hotel Guest from Danville, CA
April 19, 2016
Family Traveling with Young Children
April 19, 2016
Family Traveling with Young Children
This guest did not leave comments about this hotel.
Verified Hotel Guest from Santa Cruz, CA
March 22, 2016
Solo Traveler
March 22, 2016
Solo Traveler
The room was very large and I was able to drop the temp to a nice 62 degrees to sleep. I really liked the power outlets that were in every lamp making charging devices easy.
Would have liked more room surrounding the toilet. Also, the fridge, although large, didn't have a freezer section which I use for ice packs.
Verified Hotel Guest from Fair Oaks, CA
January 3, 2016
January 3, 2016
This guest did not leave comments about this hotel.
Verified Hotel Guest from Peoria, IL
September 4, 2015
Family Traveling with Young Children
September 4, 2015
Family Traveling with Young Children
Very nice pool area. Good breakfast. Very clean and safe area.
Reviews of Hampton Inn And Suites Folsom
(1 - 10 of 30 reviews)
(1 - 10 of 30 reviews)
from $84
- Courtyard By Marriott Sacramento Folsom from $89 (0.3mi)
- Residence Inn By Marriott Sacramento Folsom from $122 (0.3mi)
- Hilton Garden Inn Folsom from $92 (4.2mi)
- Larkspur Landing Folsom-An All-Suite Hotel from $91 (4.2mi)
- Lake Natoma Inn from $107 (4.3mi)
- Fat's Asia Bistro & Dim Sum Bar (0.4mi)
- Jack's Urban Eats (1.0mi)
- Taiko Sushi (1.2mi)
- Kirala Sushi And Japanese Cuisine (2.7mi)
- Bidwell Street Bistro (3.0mi)
- El Dorado Hills, CA (9.7 mi)
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- Rocklin, CA (12.3 mi)
- North Highlands, CA (14.7 mi)
- Shingle Springs, CA (18.5 mi)
- Auburn, CA (19.3 mi)
- Lincoln, CA (21.4 mi)
- Sacramento, CA (24.8 mi)
- Plymouth, CA (27.5 mi)
- West Sacramento, CA (27.6 mi)
- Elk Grove, CA (27.9 mi)
- Placerville, CA (28.9 mi)
Most Popular Guest-Rated Hotels in Folsom
- Residence Inn By Marriott Sacramento Folsom
- Hampton Inn And Suites Folsom
- Larkspur Landing Folsom-An All-Suite Hotel


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That said, there is some evidence to suggest it is more than just wishful thinking. On the audio drama included with the HD re-release, a continuation to the story is teased. It's in Japanese, but you can listen for yourself here.
We'll keep you updated if new details emerge. Final Fantasy X / X-2 HD Remaster will be released on March 18th in North America, and March 21st in Europe.
Tales from the Borderlands July 31 • 9:00am PDT/12:00pm EDT
Sword Coast Legends Aug 7 • 9:00am PDT/12:00pm EDT
Breath of Fire IV Continues! Every Monday • 5:00pm PDT/8:00pm EDT
Star Wars: KotOR Continues! Coming soon...


text:
Crazy Substitute Teachers
Enjoy, NG4.02 / 5.00 2,489 Views
Vacuum Salesman
This dude and his Megazord vacuum cleaner4.01 / 5.00 2,774 Views
Markiplier Animated | Mark Of The Survey Corps
This took far too long to make...3.97 / 5.00 6,743 Views
Ah hahahaha! This is Epic... I love it!!!! This Nyan Cat rules!!!!
LOVED IT! I wish all hold music was that good!
Just Fantastic
ahahah ahhh...lovely


text:
Kin: Men of Numenor
Leader: Tarnomir
Website: www.menofnumenor.webs.com
Type: Posts; User: olorin523; Keyword(s):
Kin: Men of Numenor
Leader: Tarnomir
Website: www.menofnumenor.webs.com
We also party our brains out and drink till we go blind. We do have the occasional contest with a cash reward, if you are into that sort of thing. If you would like to give us a try , Im or mail...
I like all the ideas everyone is throwing out there. I am just hoping that this will bring about improvment to the Kinship system.
Thanks Galuhad, I saw your post after I made mine. If I had seen yours, I would have supported it instead of making this one. Also thank you fulksayyan for your support. I really hope these ideas get...
Thank you, hope everyone has the same reaction.
Well, I just wanted to state a few ideas I had about improving the kinship system. I am not trying to criticize turbine, I just wanted to put forward some things to consider and possibly update. This...
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text:
"The belief generally on the Street, 'As goes oil prices, so goes stock prices,' that's nonsense," Dennis Gartman tells CNBC. » Read More
CNBC's Steve Liesman reports the Federal Reserve is leaving rates alone for now but that plan may change after Labor Day.
Ed Lee, Recode managing editor, says he is impressed with Facebook's mobile growth and advertising revenue.
Roger Altman, Evercore founder & executive chairman, makes his case for a Hillary Clinton presidency.
CNBC's Phil LeBeau breaks down Ford's mixed results which missed on earnings but beat on revenues.
Former Gov. Tim Pawlenty, (R-Minn.), talks about his support of Donald Trump and the impact of bringing back the Glass-Steagall Act.
The "Squawk Box" news team discusses some of the morning's most provocative headlines, including a record-breaking number of Harry Potter pre-orders, and Twitter lighting up social media with videos of a strange light streaking across the California sky.
Sebastien Bazin, AccorHotels CEO, discusses how terrorism is changing the way people travel, the tourism industry, and the impact of Brexit on bookings.
Victor Anthony, Axiom Capital Management, shares his thoughts on Facebook's quarterly numbers and where he sees engagement opportunities for the social media giant.
Among the events that happened on this day in history, in 1977 oil flows for the first time through the Trans-Atlantic Pipeline.
Buffalo Wild Wings' stock spiked 10 percent after Marcato Capital Management acquired a 5.1 percent stake in the company earlier in the week.
CNBC's Jim Cramer is keeping his eye on Apple after the tech giant beat expectations. Also Cramer weighs in on Boeing and Coca-Cola.
Bassem Youssef, political satirist, discusses his new web series, "The Democracy Handbook" and taking on the political satire scene.
CNBC's Meg Tirrell breaks down GlaxoSmithKline's quarterly results and provides highlights from CEO Andrew Witty comments to CNBC Europe on Brexit.
Gov. John Hickenloop, (D-Colo.), shares his thoughts on free trade and whether the Trans-Pacific Partnership agreement is good enough for American workers.
Gov. John Hickenlooper, (D-Colo.), shares his thoughts on Hillary Clinton's running mate.
CNBC's Rick Santelli breaks down the latest data on the U.S. economy.
Nicholas Burns, former amabassador to NATO, discusses the importance of working with our allies to fight the war of terror.
J.B. Pritzker, The Pritzker Group, says he is "comfortable" with most of the Democratic agenda but does not agree with Donald Trump's stance on immigration among other things.
Ian Ziering, actor, talks about his character Fin Shepard in the SyFy movie series "Sharknado."
Ultimately the voters are going to decide this election, says Rep. Chris Collins, (R-N.Y.), making the case for Donald Trump.
Get the best of CNBC in your inbox
Joe Kernen is co-anchor of "Squawk Box," CNBC's signature morning program.
Becky Quick is co-anchor of "Squawk Box" and also anchor of the nationally syndicated "On the Money."
Andrew Ross Sorkin is a co-anchor of "Squawk Box," a financial columnist for "The New York Times" and the editor-at-large of NYT's DealBook.


text:
Pharmacists and law enforcement officials in Nassau County will soon meet to discuss ways to improve store security.
This follows two fatal pharmacy robberies on Long Island in 2011.
Nassau County officials will meet with members of the Long Island Pharmacy Crimes Task Force on Feb. 7 in Bethpage.
Related stories
- No charges in NY ATF agent's friendly-fire death
- Victim's kin in LI pharmacy killing announce suit
- Nassau to hold forum on pharmacy safety
Police and prosecutors will discuss their efforts investigating and deterring drug store holdups.
Also, a training session for pharmacists will be held the following month.
Both meetings are at Nassau County's Morrelly Homeland Security Center in Bethpage.
An off-duty ATF agent was killed in a suspected "friendly fire" shooting while trying to stop a robbery in a Seaford pharmacy on New Year's Eve.
Last June, a gunman shot and killed four people during a robbery at a Medford pharmacy.
More breaking news
- PSEG Long Island activates AC program to reduce peak load
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- LI unemployment falls to 3.8% near historic lows
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- Sweet deal
- Verizon 2Q profit hurt by strike; revenue disappoints
- US new-home sales climbed in June to more than 8-year high
- Opening fete for Wyandanch Domino's
- Banquet facility agrees to pay $50K in overtime dispute


text:
Narendra Modi govt "disrespects" people's mandate: Sonia GandhiState Congress president Ashok Chavan, former chief minister Prthviraj Chavan, former Union minister S K Shinde and other senior leaders from the state were present. (Reuters) Seizing on the Supreme Court verdicts on Arunachal Pradesh and ... Financial Express, 1 week ago
Modi govt destroying Constitutionand co-operatives, fumes Sonia - The Freepress Journal, 1 week ago
Modi govt "disrespects" people's mandate: Sonia - Deepika Global, 1 week ago
1 images for "power minister shinde"
Maharashtra: CM Devendra Fadnavis inducts 11 more ministers
Out of the 11 ministers 10 were new faces, while one BJP Minister of State, Ram Shinde, was elevated to the Cabinet rank. The Cabinet expansion in Maharashtra saw 11 ministers taking oath Friday as the BJP government gave a greater representation to ...Indian Express, 2 weeks ago Fadnavis announces portfolios of new ministers Times of India, 2 weeks ago Maharashtra cabinet reshuffle today: Fadnavis to get in 10 new ministers Hindustan Times, 2 weeks ago Fadnavis pledges help in aquifer mapping for a clean Nandini Times of India, 1 month ago
#dnaEdit: Power games
Chief Minister Devendra Fadnavis seems to have mastered the art of dealing with the high-strung Shiv Sena, the junior coalition partner. Fadnavis had conceded only two minister-of-state berths to the Sena, rejecting Uddhav Thackeray's demand for a ...DNA India, 2 weeks ago Sena divided over joining Fadnavis cabinet? Afternoon Despatch & Courier, 2 weeks ago
Goyal, Chidambaram set to be elected to RS from Maha
Mumbai, May 31 (PTI) Union Power Minister Piyush Goyal and senior Congress leader and former Finance Minister P Chidambaram are set to be elected to Rajya Sabha from Maharashtra, with the deadline for filing nominations ending today. They are among ...Press Trust of India, 1 month ago Piyush Goyal, Chidambaram all set to enter RS from Maharashtra Firstpost.com, 1 month ago Chidambaram is Cong choice for RS seat from Maharashtra Times of India, 1 month ago Piyush Goyal, Chidambaram Set To Be Elected To Rajya Sabha From Maharashtra NDTV, 1 month ago
More from: NDTV, Outlook India...and 2 other sources
Mumbai-Pune Expressway accident: Eknath Shinde assures free treatment to injured
PWD minister and guardian minister of Thane district, Eknath Shinde, visited the MGM Hospital in Kamothe and assured the injured passengers of free and speedy treatment. "Our first priority is to ensure treatment of the injured. We would provide all ...DNA India, 1 month ago 17 die, 43 injured as bus rams into cars on Mumbai-Pune Expressway DNA India, 1 month ago 17 killed in Mumbai-Pune expressway accident India Online.in, 1 month ago
Senior Congress leaders advice dropped Ministers not to get dejected
Bengaluru, June 25 : Two Senior Congress leaders, who were dropped from the Siddaramaiah Ministry in Karnataka during this week's reshuffle today advised their former colleagues not to get dejected over their exit from the cabinet and take a ...Newshence.com, 1 month ago Trouble for Congress in Karnataka and Chhattisgarh India Today, 1 month ago Pressure builds on BJP to ban shadowy Sanstha Times of India, 1 month ago Congress fields Rane in Maharashtra council polls Yahoo! India, 1 month ago
HT Exclusive: Maha ministers flout rules, get red beacons on pvt cars
To continue reading, we request you to support us by disabling your Ad Blocker In order to serve content on our website, we rely on advertising revenue which helps us to ensure that we continue to serve high quality unbiased journalism. To know ...Hindustan Times, 2 weeks ago
Galaxy of leaders greet Gadkari on his birthday
Nagpur, May 27: Union Surface Transport, Highways and Shipping Minister Nitin Gadkari today celebrated his 58th birthday at his Mahal-based residence. A galaxy of prominent personalities from all walks of life lined up to greet the him. However,the ...Newshence.com, 2 months ago
Kavita Kaushik: We need better shows on TV
There is more to Kavita Kaushik than just being a powerhouse performer. Kavita Kaushik's Dabangg avatarThe entire country is a fan of Dabangg superstar Salman Khan and Kavita Kaushik is no different! This was proved during one of the matches of ...Times of India, 2 months ago From a Haryanvi cop to a Rajasthani doctor Kavita Kaushik's back in uniform Times of India, 2 months ago
Practitioners of politics of hatred can take away power, not ideology: Sonia
New Delhi, May 21 (IANS) Indirectly attacking the BJP, Congress President Sonia Gandhi on Saturday said the practitioners of "politics of hatred" may have deprived the party of political power, but they can't take away from it the ideology that ...Yahoo! India, 2 months ago
More from: , OdishaSunTimes.com...and 2 other sources
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Crazy Substitute Teachers
Enjoy, NG4.03 / 5.00 2,640 Views
Vacuum Salesman
This dude and his Megazord vacuum cleaner4.01 / 5.00 2,902 Views
Markiplier Animated | Mark Of The Survey Corps
This took far too long to make...3.96 / 5.00 7,540 Views
It's an honor and a privilege to be able to watch even another moment of Prosnorkulus' amazing and hyperviolent adventures. I now understand that waiting so long between adventures was for my safety, since having watched Episode 2 after waiting less than a full year since Episode 1 made my left eardrum explode and my right eye swell to the size of an orange from the sheer force of awesomeness of Prosnorkulus' extremely manly antics. I thank you, as watching this instructional installment has made me able to cleave bison in half with one stroke and cause small animals to explode by scowling.
I will be glad to sacrifice my health further for any future episodes. Generations yet to come will thank you for your telling of these tales, if there are any that survive viewing them.
Too much Face exploding not enough crazy cut scenes with crazy tunes.
PRO!snorkulus rules... Ooooaaah.
This video makes Newgrounds history. Damn SON!
needs more frames, better fluency of animation, overall great


text:
Arsenal prepare £50m bid for Luis Suarez, Wenger still has £70m [Daily Telegraph]
Sometimes it is what's written between the lines that is more interesting than the headline or opening paragraphs.
Jeremy Wilson in the Daily Telegraph today confirms that Arsenal are closing in on the 14 million pound signing of Brazilian midfielder Luiz Gustavo from Bayern Munich.
Gustavo is expected to join Arsene Wenger's side before the start of the Premier League season on Saturday and will provide plenty of competition for the likes of Mikel Arteta, Jack Wilshere, Aaron Ramsey and Abou Diaby.
It is what Wilson writes further down that will be of more interest to Arsenal fans and those of a Liverpool persuasion as well.
With Sky Sports News claiming Luis Suarez has no intention of apologising to his manager and team mates – despite calls from Brendan Rodgers on Saturday (see video below) – surely Liverpool will sell if they receive a bid of 50 million pounds, right?
Or, should Arsenal hold out for Wayne Rooney?!
Watch Live Stream
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Latest on Arsene Wenger
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Latest on Brendan Rodgers
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text:
Wyclef moves on from April Showers and preps us for his upcoming album, The Carnival Returns.
Wyclef Jean – Bagay Nef
Snow Tha Product - "Alright" f. PnB Rock
The soundtrack to every dysfunctionally functioning relationship.
Kooley High - "Alone" (Video)
From their project Heights, which is out now.
Phantogram - "You Don't Get Me High Anymore"
The first single from their yet-to-be-named album.
Azekel - "Linger"
The British singer/producer returns with the first Raw, Vol. 2 single.
Stream Disclosure's Surprise EP, 'Moog For Love'
Wait until you hear their Al Green flip...


text:
Samsung Galaxy Tab Active is Sammy's toughest slate with stylus yet
Samsung has announced a new member of its Galaxy Tab family and this one in a toughie, the Samsung Galaxy Tab Active.
The Active is the same name given to the tough version of the Galaxy S5. Like that, this tablet is also built to withstand anything.
The Galaxy Tab Active is IP67 rated meaning it can survived a 1.2-metre drop while also being water and dust resistant – meaning it can be left on a beach or dunked underwater without any worries.
The Tab Active has an 8-inch TFT screen running a 1280 x 800 resolution for a 188ppi. It's powered by a 1.2Ghz quad-core processor backed by 1.5GB of RAM. There's 16GB internal storage expandable to by a further 64GB using microSD. On the rear is a 3.1-megapixel auto focus camera with flash and on the front is a 1.2-megapixel selfie camera.
The tablet comes with the Android 4.4 KitKat operating system, USB 2.0, Bluetooth 4.0, GPS with GLONASS.
It also features a 4,450mAh battery that Samsung says gives 8 to 10 hours of use. This can be removed so for those carry spare that battery life can be double or more.
The tablet also features Samsung's C-Pen stylus, ideal for those working with documents or wishing to draw using the tablet. But it's aimed more at those working outdoors with gloves that still want to use the touchscreen.
The Galaxy Tab Active comes in two connectivity options: Wi-Fi only or Wi-Fi and 4G LTE using a SIM.
The tablet is aimed at business sales so release date and pricing have not yet been announced. Here's hoping anybody can get their hands on it soon.
READ: Dyson 360 Eye: Robotic vacuum cleaner coming to the UK 2015
Best smartphone battery packs: Pokemon power and more
Huawei MateBook review: 5-hours of friendship
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text:
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2331 days ago
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Showing 1 to 4 of 4 comments.
- 1
Ryanknoll2 - 2328 days ago
he grips only the shaft, which means that the orgasm is produced by the cotton rubbing against the head. wonderful.
jempiecam - 2329 days ago
wow i love to see playing in undies and see cum it so hot have to wank in my shorts to.
stevesw - 2331 days ago
I love seeing guys wearing white pants - it does it for me! And to see cum dribbling down leg is such a turn-on. Super sized cock and ball sac fills your pants. Love to play with you
- 1


text:
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text:
I'm MJM's very first customer since they started.
I want to say that I shop at the other sites also, but MJM is still my favorite that I go...
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I must say, I was quite impressed with MJM Magic my first time ordering through them! A friend of mine referred me to the site, and at first I was...
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Yes, MJM Magic has great prices and very fast service, but it is also one of the few online magic shops that accept Paypal.
For those without a...
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text:
Man charged with attempted murder in Aylesbury
- 7 March 2013
- From the section Beds, Herts & Bucks
A 38-year-old man has been charged with attempted murder after a woman was attacked in Aylesbury.
Police were called to a house in Belgrave Road at 11:40 GMT on 5 March.
Darren Stenhouse, who lives in the street, was arrested following a search of the surrounding area and will appear before magistrates on Thursday.
The 30-year-old victim sustained head and arm injuries. She has since been discharged from hospital.


text:
Three-star receiver Shay Fields Jr. (Bellflower, Calif./St. John Bosco) piled up 145 receiving yards and four touchdowns Friday night with USC coaches Tee Martin and Ed Orgeron in attendance. Less than four days later, Fields hauled in something else: a surprise scholarship offer from the Trojans.
Adoree' Jackson makes his case for Rio ... as a gymnast?
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8dTed Miller
USC's Adoree' Jackson performs a perfect wall flip
8dDavid Lombardi
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15dSarah Scrivens


text:
Inspired by the groundbreaking work of Jason Mittel and Christian Keathley, among others, of inTransition (http://mediacommons.futureofthebook.org/intransition/), Film Matters is excited to announce our first call for videographic film scholarship by undergraduate students — an initiative managed by Allison De Fren and Adam Hart.
For more information about this opportunity, including specific instructions for formatting submissions, please download the official document:
The deadline is October 1, 2016.
Questions and submissions should be directed to Allison De Fren and Adam Hart at: VideographicFM AT gmail.com
And for a recent example of videographic criticism, published by Film Matters, please consult the following from Jennifer Fleeger and Jordan Scharaga:
Scoring Night and the City. By Jennifer Fleeger and Jordan Scharaga
We look forward to receiving your video submissions!


text:
BioActive Grass Juice Powders
Our young, tender, and vibrant grasses are harvested at the very zenith of their nutritional potential, which are then immediately and carefully juiced at a low temperature to ensure there is no degradation to the raw living properties of the grasses. All our grass juice powders are produced purely from grasses organically grown in some of the richest soils in the the world, located in an ancient sea bed in Utah, USA.Learn More
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BioActive Blue Green Algae Powder - Wild Harvested from Klamath Lake
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text:
Integreon Expands Global Delivery Center In Fargo, ND
The facility will consolidate almost 350 Integreon Associates from two existing facilities in Fargo.
Target Corporation To Locate New Online Fulfillment Center In Memphis, TN
Fortune 50 retailer to invest $50 million; will create more than 400 new jobs.
Arotech Combines Facilities Into One Location In South Carolina
The South Carolina location is the home base of UEC Electronics, which Arotech acquired in April 2014.
Ex-Im Bank Joins With Virginia Small Business Development Center To Boost Exports
The program is expected to accelerate export business and job growth by connecting Bank products and services to a statewide network of Small Business Development Centers (SBDCs).
Kubota Tractor To Open New Distribution Center In Edgerton, KS
Kubota plans to have the facility operational during the third quarter of 2015.
Subaru Looking To Move HQ From Cherry Hill To Camden, NJ
Final decision is expected by April 2015 and subject to this approval, Subaru plans to complete the project by fourth quarter, 2016 or early 2017.
M-TEK Locating New Headquarters In Murfreesboro, TN
New North American Headquarters for automotive supplier will employ 250, with 100 new jobs.
California's "Coolest" Cities Announced
CoolCalifornia City Challenge recognizes the best cities in the state--for energy management.
QTS Completes Phase I Construction Of Dallas-Fort Worth Data Center
With 12 data centers in eight states, QTS owns, operates and manages approximately 4.7 million square feet of data center infrastructure and supports more than 850 customers.
Fisher Dynamics Selects Evansville For First Indiana Operation
Michigan-based auto seat mechanism supplier chooses Indiana for new operations.
Broken Bow II Wind Farm Dedicated As Nebraska's Newest Clean Energy Project
Construction on the new wind farm began in January 2014. It provided a boost to the local economy by creating more than 300 jobs during peak construction.
Lockheed Martin Opens Commercial Space Headquarters In Denver, CO
The event signifies the official transition of the Lockheed Martin facility in Newtown, PA to Denver, CO as part of a phased transition announced last year.
Israeli Agtech Company Chooses St. Louis, MO As U.S. Headquarters
BioSTL's newly launched St. Louis-Israel Innovation Connection (SLIIC) initiative establishes a direct pipeline for business interaction between St. Louis and the country of Israel.
Bridgestone Opens Off Road Radial Tire Manufacturing Facility In Aiken County, SC
South Carolina plant extends company leadership in large and ultra-large earthmoving tires for mining and aggregates applications.
Greenway Health LLC Building New Cobb County, GA Tech Development Center
Health IT company will bring 150 jobs to the metro Atlanta area with its new technology development center.
CompuGroup Medical US Relocating From Boston To Phoenix
The existing office in Phoenix will be co-located with the new head office and will serve as a unified location for human resources, research and development, product management, marketing and sales.
Linde Process Plants Invests In Expansion At Port Of Catoosa
Company eyes growth opportunity, increased productivity and enhanced safety; projected completion date for the new structure is June 2015.
International Economic Development Council Announces Award Winners At Annual Conference
IEDC's Excellence in Economic Development Awards recognize the world's best economic development programs and partnerships, marketing materials, and new media.
Signature Systems Group Relocating Headquarters To Dallas, TX
The new facility will provide room for future organizational growth and will feature a state-of-the-art distribution center and product showroom.
Alcoa Announces Investment In Iowa Aerospace Manufacturing Plant
Davenport, IA facility positions Alcoa to continue capturing growth in aerospace and industrial sectors.


text:
Harbour Vue Guest House, Western Cape (province)
- Free English breakfast, free WiFi, and free parking
Main amenities
- 5 guestrooms
- Near the beach
- Outdoor pool
- Airport shuttle
- Self-serve laundry
- Tour/ticket assistance
Feel at home
- Private bathroom
- Separate dining area
- Separate living room
- Separate sitting area
- Television
- Laundry facilities
What's around
- Whalehaven Winery (2.5 mi / 4.1 km)
- Hermanus Beach (4.6 mi / 7.4 km)
- Fernkloof Nature Reserve (5.6 mi / 9 km)
- Bot River Estuary (10.3 mi / 16.6 km)
- Kleinmond Beach (21.1 mi / 34 km)
Harbour Vue Guest House, Western Cape (province)
Hotel Info: 866-539-8117from $42
- Luxury Double Room, Mountain View
- Double Room, Sea View (Southern Right)
- Double Room, Balcony
- Luxury Double Room, Sea View
Also popular with travelers from the USA
At a glance
Key facts
Hotel size
- This hotel has 5 rooms
- This hotel is arranged over 2 floors
- Check-in time 2 PM-4 PM
- Check-out time is 11 AM
Required at check in
- Credit card or cash deposit required
- Government-issued photo ID required
- Minimum check-in age is 15
- Minimum Spring Break check-in age is 15
- Free WiFi in public areas
- Free WiFi in rooms
- Airport shuttle during limited hours *
- Free self parking
Other information
- Resort fee included
Nearby Four Star Western Cape (province) hotels
In the hotel
- Free English breakfast daily
- Outdoor pool
- Tours/ticket assistance
- Laundry facilities
- Number of buildings/towers - 1
- Year Built 2005
In the room
- Ceiling fan
- Coffee/tea maker
- Premium bedding
- Sleep Number by Select Comfort mattress
- Individually decorated
- Separate dining area
- Separate living room
- Separate sitting area
- Balcony
- Private bathroom
- Deep soaking bathtub
- Rainfall showerhead
- Desk
- Free WiFi
- In-room safe
Top Western Cape (province) hotels
Recently reviewed hotels in Western Cape (province)
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Nearby hotels
Harbour Vue Guest House, Western Cape (province)'s small print
Also known as
- Harbour Vue
- Harbour Vue Guest House
- Harbour Vue Guest House Hermanus
- Harbour Vue Hermanus
For transfers, guests must contact the property before arrival at the number on the booking confirmation.
The name on the credit card used at check-in to pay for incidentals must be the primary name on the guestroom reservation.
Mandatory fees
The charges below are included in the total price displayed:
- Resort fee
Optional extras
Airport shuttle service is offered for an extra charge
We have included all charges provided to us by this hotel. However, charges can vary, for example, based on length of stay or the room you book.
Recent reviews
Outstanding 4.7 /5 from 30 reviews
Barry, the Manager is a star. He is the most friendly host ever. Nothing was too much. The Vue is a wonderful, relaxing and well located BnB. It does not have an elevator, so not good for some. Breakfast is to die for. Hope to teturn
Perfect location if you're looking for a quieter hotel away from the more touristy town centre. Ideal for watching whales and strolling along the cliff walk into the town. Incredibly helpful staff.
We had the Southern Right room and it was perfect! We had spectacular views to the bay and we could see whales while enjoying our morning coffee. Barry, the manager, is an absolute sweetheart. He helped us with everything, from directions, restaurant advice, and booking a whale watching tour. I would love to return to Hermanus and when I do I'll be staying at Habour Vu.
There is very little I can say poor about this establishment. It is a mansion of about 6-8 rooms, however not all of them have a sea view. For the people who do not have a seaview, there is an upstairs lounge with a honesty bar and a beautiful view of the sea. The bedding and rooms furnishings were top class quality and the bathroom was very modern. It is very central and close to restaurants and in a safe area. The breakfast was excellent and even had home made breads and preserves. Wifi was present and free
pleasant comfortable and wonderful place delightful host made us feel very comfortable will recommend to friends
Is the description of this hotel not correct? Tell us


text:
May 23, 2014
A rich man wanted to get married, but he was having trouble choosing among three candidates. As a test, he gave each woman $5,000 to see what she would do with it.The first woman got a total makeover. She went to a fancy beauty salon, got her hair and makeup done, bought several new outfits, and dressed to the nines for the man. She told him that she did this to be more attractive to him because she loved him so much.The second woman went shopping. She bought the man a new set of golf clubs, some new high-tech toys, and some expensive clothes. She told him that she spent all the money on him because she loved him so much.The third woman invested the money in the stock market and earned back the investment several times over. She gave the man back his $5,000 and reinvested the rest in a joint account. She told the man that she wanted to save for their future because she loved him so much.The man reflected for a long time about what each woman had done with the money he'd given her.Then he married the one with the biggest chest.
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text:
Lyrics Depot is your source of lyrics to Thy Servant by Old Man's Child. Please check back for more Old Man's Child lyrics.
Thy Servant Lyrics
Artist: Old Man's Child
Album: Ill-Natured Spiritual Invasion
A being of evil nature son of deaths creator
I am the one you fear the master of your despair
Raised under satans spell inspired by the judgement bells
now I am at war I'll make you Satan's whore
The devil spawn of demoniac breed in a ghostly form by evil seed
I am the anti-Christ I am the anti-life
Marked by the sign of the beast I roam
the streets unleashed praised be the one, the evil son
The blood of Christ
Shall be your defeat
On the crucifix
You will meet your God
Old Man's Child Lyrics
Old Man's Child Ill-Natured Spiritual Invasion Lyrics
More Old Man's Child Music Lyrics:
Old Man's Child - Behind The Mask Lyrics
Old Man's Child - Born Of The Flickering Lyrics
Old Man's Child - Captives Of Humanity Lyrics
Old Man's Child - Christian Death Lyrics
Old Man's Child - Felonies of the Christian Art Lyrics
Old Man's Child - Into Silence Embrace Lyrics
Old Man's Child - King Of The Dark Ages Lyrics
Old Man's Child - Return Of The Nights Creature Lyrics


text:
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text:
|Scientific classification
About 35.
Arthrospira is a genus of free-floating filamentous cyanobacteria characterized by cylindrical, multicellular trichomes in an open left-hand helix. There is a dietary supplement made from Arthrospira platensis and Arthrospira maxima, known as Spirulina.[1] The maxima and platensis species were once classified in the genus Spirulina. ″Although the introduction of two separate genera [Arthrospira and Spirulina] is now generally accepted, there has been much dispute in the past and the resulting taxonomical confusion is tremendous″.[2] ″
The maxima and platensis species were once classified in the genus Spirulina. The common name, Spirulina, refers to the dried biomass of Arthrospira platensis,[3] which belongs to the oxygenic photosynthetic bacteria that cover the groups Cyanobacteria and Prochlorales. These photosynthetic organisms, Cyanobacteria, were first considered as alge until 1962 and for the first time, these blue green algae were added to prokaryote kingdom and proposed to call these microorganisms as Cyanobacteria [4] where algae is considered to be a very large and diverse group of eukaryotic organisms. This designation was accepted and published in 1974 by the Bergey's Manual of Determinative Bacteriology.[5] Scientifically, there is a quite distinction between Spirulina and Arthrospira genus. Stizenberger, in 1852 gave the name Arthrospira based on the septa presence, helical form and multicellular structure and Gomont in 1892, confirmed aseptate form of the Spirulina genus. Geitler in 1932, reunified both members designating them as Spirulina without considering the septum.[6] The worldwide research on microalgae was carried out in the name of Spirulina, but the original species exploited as food with excellent health properties belongs to genus Arthrospira. This common difference between scientists and customers is difficult to change.[5] These Arthrospira genus, constitute a helical trichomes of varying size and with various degree of coiling including tightly coiled morphology to even straight uncoiled form. The filaments are solitary and reproduce by binary fission and the cells of the trichomes vary from 2 μm to 12 μm and can sometime reach up to 16 μm. Species of the genus Arthrospira have been isolated from alkaline brackish and saline waters in tropical and subtropical regions. Among the various species included in the genus Arthrospira, A. platensis is the most widely distributed and is mainly found in Africa but also in Asia. Arthrospira maxima is believed to be found in California and Mexico.[6] They are now agreed to be in fact Arthrospira; nevertheless, and somewhat confusingly, the older term Spirulina remains in use for historical reasons.[1][7] However, current taxonomy claims that the name ′Spirulina′ for strains used as food supplements is inappropriate, and there is agreement that Arthrospira is a distinct genus, consisting of over 30 different species including A. platensis and A. maxima″.[8]
Arthrospira platensis and Arthrospira maxima occur naturally in tropical and subtropical lakes with high pH and high concentrations of carbonate and bicarbonate.[9] Arthrospira platensis occurs in Africa, Asia and South America, whereas Arthrospira maxima is confined to Central America and Arthrospira pacifica is endemic to Hawaii Islands of America.[7] Most cultivated spirulina is produced in open channel raceway ponds, with paddle-wheels used to agitate the water.[9] The largest commercial producers of spirulina are located in the United States, Thailand, India, Taiwan, China, Pakistan, Burma (a.k.a. Myanmar), Greece and Chile.[7]
See also[edit]
- ^ a b Ciferri, O. (1983). "Spirulina, the edible microorganism". Microbiological reviews 47 (4): 551–578. PMC 283708. PMID 6420655.
- ^ Mühling, Martin (March 2000). Characterization of Arthrospira (Spirulina) Strains (Ph.D.). University of Durham. Archived (PDF) from the original on 2016-01-23. Retrieved 2016-01-23.
- ^ Gershwin, ME; Belay, A (2007). Spirulina in human nutrition and health. CRC Press, USA.
- ^ Stanier, RY; Van Niel, Y (January 1962). "The concept of a bacterium". Arch Mikrobiol 42: 17–35.
- ^ a b Sánchez, Bernal-Castillo; Van Niel, J; Rozo, C; Rodríguez, I (2003). "Spirulina (arthrospira): an edible microorganism: a review". Universitas Scientiarum 8 (1): 7–24.
- ^ a b Siva Kiran, RR , Madhu GM*, Satyanarayana SV (2016). "Spirulina in combating Protein Energy Malnutrition (PEM) and Protein Energy Wasting (PEW) - A review". Journal of Nutrition Research. Retrieved February 20, 2016.
- ^ a b c Vonshak, A. (ed.). Spirulina platensis (Arthrospira): Physiology, Cell-biology and Biotechnology. London: Taylor & Francis, 1997.
- ^ Takatomo Fujisawa, Rei Narikawa, Shinobu Okamoto, Shigeki Ehira, Hidehisa Yoshimura, Iwane Suzuki, Tatsuru Masuda, Mari Mochimaru, Shinichi Takaichi, Koichiro Awai, Mitsuo Sekine, Hiroshi Horikawa, Isao Yashiro, Seiha Omata, Hiromi Takarada, Yoko Katano, Hiroki Kosugi, Satoshi Tanikawa, Kazuko Ohmori, Naoki Sato, Masahiko Ikeuchi, Nobuyuki Fujita, and Masayuki Ohmori (2010-03-04). "Genomic Structure of an Economically Important Cyanobacterium, Arthrospira (Spirulina) platensis NIES-39". Oxford University Press. PMC 2853384. In its turn, it references: Castenholz R.W.; Rippka R.; Herdman M.; Wilmotte A. (2007). Boone D.R., Castenholz R.W., Garrity G.M., eds. Bergey's Manual of Systematic Bacteriology (2 ed.). Springer: Berlin. pp. 542–3.
- ^ a b Habib, M. Ahsan B.; Parvin, Mashuda; Huntington, Tim C.; Hasan, Mohammad R. (2008). "A Review on Culture, Production and Use of Spirulina as Food dor Humans and Feeds for Domestic Animals and Fish" (PDF). Food and Agriculture Organization of The United Nations. Retrieved November 20, 2011.
External links[edit]
Guiry, M.D.; Guiry, G.M. (2008). "Arthrospira". AlgaeBase. World-wide electronic publication, National University of Ireland, Galway.


text:
I need to use a name of file as an argument in linux shell command. The problem is, java gives me that name as it is, saving all that spaces and other characters, and thus shell complains. Is there a method to escape all those problematic characters before passing the string to shell?
We started with Q&A. Technical documentation is next, and we need your help.
Whether you're a beginner or an experienced developer, you can contribute.
You should be able to put single quotes around the argument and avoid escaping it altogether. Will that work for you?
Old: myapp -f /bad/path/to/file
New: myapp -f '/good/path/to/file'
How about using the Exec module from Apache Commons? It includes a commandline builder. Also be aware that if the filename is retrieved from user input, you should be very careful with executing commands with the user input as a program argument. Escaping improperly may lead to execution of additional commands (unless the commons module is used I guess).
Had the same problem, single quotes wasn't sufficient (as already pointed out by Robert)
Why such an awfully complex replacement? That's why.
Use case:
Works as intended:
Adding quotes single or double around the file name is often sufficient, depending on the characters you have in the name it may not.


text:
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text:
Daily News Where you Live
Other Pamplin Media Group sites
Average Portland home price hits record $412,000
Pierce relying on GOP donors to fund gubernatorial campaign
Environmentalists shut out of Oregon forest litigation
Time runs out for Oregon timber ballot initiatives
School Notes: Low-income PCC students to get $1.3 M in services; Students take on the world in storytelling event
Lodi crushes Pickles in opener
Paxton pitches Mariners to win
PREPS: Beaver believer -- Aloha star pitcher Brandon Eisert looks forward to Oregon State career//
Up-and-coming bull rider Chase Dougherty living the dream
Vance, 3 others join Concordia men's basketball; Hops lose, 2-0; GreyWolves top Corvallis, 5-3
First wolves born in Oregon since 1940s
Wolf pups were spotted by biologists this week in the Rogue River-Sikiyou National Forest in southwest Oregon, the first confirmed wolves born in the Oregon Cascades since the mid-1940s.
A wild wolf known as OR7, which migrated from northeast Oregon, was spotted earlier by remote cameras with a litter of wolf pups, and a black female wolf was spotted in the same area.
Biologists from the U.S. Fish and Wildlife Service and Oregon Department of Fish and Wildlife trekked into the area and observed two pups. They say its likely there are more pups, since wolf litters typically number four to six pups.
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