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Interview with prolific paranormal, suspense, and mystery novelist Tanya R. Taylor author of 10 Minutes Before Sleeping, Cornelius, Haunted Cruise, and many others. Read More »
How Paranormal Reality Entertainment Is Changing Culture and Beliefs
Paranormal investigating has turned into big profits for everybody involved – except for the independents, the ones with their YouTube channels and blogs. And that's where the best paranormal reality can be found. Read More »
Theater Reviews (NYC): 'Sprucehaven B' and 'Emily Dickinson: Paranormal Investigator' at FRIGID New York
There's violence in Isabel's past, and now things seem to be spiraling out of control again. Ultimately, an edgy situation turns into a nightmare in Mark Cornell's chilling 'Sprucehaven B.' Read More »
EXCLUSIVE INTERVIEW: Todd Brian Backus, PowerOut Artistic Producer, on His New Play 'Emily Dickinson: Paranormal Investigator'
A 19-year-old Emily Dickinson, teamed with Edgar Allan Poe, is the last line of defense between the everyday and a world of magic, ghosts, and vampires. Read More »
Interview: Terry Jackman, Author of 'Ashamet, Desert-Born'
"For me, the most boring books are those that tick boxes and slavishly follow an initial outline. I think a good story is often organic. . ." Read More »
Interview: 'The Whispers' Executive Producer Zack Estrin
Blogcritics exclusive interview with the executive producer of ABC's new series "The Whispers." Read More »
Interview: Joel Fox, Author of 'The Mark on Eve'
"While I am not writing history, the feel of the period must be accurate for the readers." Read More »
Book Review: 'The Hangman,' Paranormal Horror by Dee Rose
A quick-paced paranormal thriller-horror novel full of action and suspense, sprinkled with sarcasm and humor throughout the pages. Read More »
Interview: Jonathan L. Ferrara, Author of 'The Blackwell Family Secret: The Guardians of Sin'
"Writing a book is wonderful is many ways, but there are times you just want to throw it right out the window." Read More »
Book Review: 'The Blackwell Family Secret: The Guardians of Sin' by Jonathan L. Ferrara
Ferrara is a talented writer, his prose smooth and his dialogue witty. The pace was excellent and the story kept moving forward with increasing tension until the very satisfying ending that left me hungry for book 2. Read More »

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...Currently, I am a home maker and assure that I shall devote my time and be committed to educate students and share knowledge about all that I have learnt in my schools. Teaching has always been my passion and I believe that I learn while I educate others. Back home in India I have taught students from grade 1 to 10th.
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...I managed to place in top three a few time in the local races. I have taught others how to sail. I have some photography experience, including some amateur Astronomy photos of the Hale-Bop comet and other sky features.
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Comments about 'Utah Jazz: Derrick Favors to start, Butler reunion for Hayward and Stevens, Olynyk on Stockton'
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Farmington, UT
I wonder why Gordon Hayward struggles so. He is so fun to watch when he plays well and it's hard to see him not playing near his potential these days. They say Derek Favors is sorely needed, but we have several guys who can - and do - play big in the middle. I'll bet ya it's Gordon who makes the bigger difference; hope he gets his groove on soon.
- 3:14 p.m. Feb. 24, 2014
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Saint George, UT
Gordon Hayward's problem is a poor coach.
- 4:40 p.m. Feb. 24, 2014
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Los Olivos, CA
Part of Hayward and the coaching problems are related to a point guard who takes 16 shots and only has three assists. When will Trey learn to pass instead of shoot?
- 8:45 a.m. Feb. 25, 2014
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Crazy Substitute Teachers
Enjoy, NG4.03 / 5.00 812 Views
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Touching and sad.
liked the concept of how the time he spent going forward in time was in a limbo like world, and choice of music helped pull this off without voices. Great job!
My favorite NATA flash so far. The very end is confusing to me. *Spoiler, obviously* Is that HIM or HER? Out in the rain I mean. The...stone. ;)
Or maybe this is what you're talking about when you say "your own conclusions"...Great flash though. So, so awesome in every way.
the HERP DERP part made my day


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Cassirer's Metaphysics of Symbolic Forms: A Philosophical Commentaryby Thora Ilin Bayer, Ernst Cassirer
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Pub. Date: 01/28/2001
Publisher: Yale University Press
Thora Ilin Bayer focuses on the meaning of Cassirer's claim that philosophy is not itself a symbolic form but the thought around which/i>
This book -- the first commentary on Ernst Cassirer's The Metaphysics of Symbolic Forms -- provides an introduction to the metaphysical views that underlie the philosopher's conceptions of symbolic form and human culture.
Thora Ilin Bayer focuses on the meaning of Cassirer's claim that philosophy is not itself a symbolic form but the thought around which all aspects of human activity are seen as a whole. Underlying the symbolic forms are Cassirer's two metaphysical principles, Spirit (Geist) and Life, which interact to produce the reality of the human world. Bayer shows how these two principles of Cassirer's early philosophy are connected to the phenomenology of his later philosophy, which is focused on his conception of "basis phenomena" -- self, will, and work. Ultimately Cassirer conceives his philosophy as a form of the ancient Socratic quest for human self-knowledge, wherein the self makes its own nature through the power of symbolism to create the distinctively human works that constitute culture.
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Current address: Novartis Vaccines and Diagnostics, Emeryville, California, United States of America
Conceived and designed the experiments: KG DP SBP. Performed the experiments: KG DVG RB RS SJD. Analyzed the data: KG DVG RB RS SJD SBP. Wrote the paper: KG DVG SJD SBP.
The authors have declared that no competing interests exist.
Distinct prion strains often exhibit different incubation periods and patterns of neuropathological lesions. Strain characteristics are generally retained upon intraspecies transmission, but may change on transmission to another species. We investigated the inactivation of two related prions strains: BSE prions from cattle and mouse-passaged BSE prions, termed 301V. Inactivation was manipulated by exposure to sodium dodecyl sulfate (SDS), variations in pH, and different temperatures. Infectivity was measured using transgenic mouse lines that are highly susceptible to either BSE or 301V prions. Bioassays demonstrated that BSE prions are up to 1,000-fold more resistant to inactivation than 301V prions while Western immunoblotting showed that short acidic SDS treatments reduced protease-resistant PrPSc from BSE prions and 301V prions at similar rates. Our findings argue that despite being derived from BSE prions, mouse 301V prions are not necessarily a reliable model for cattle BSE prions. Extending these comparisons to human sporadic Creutzfeldt-Jakob disease and hamster Sc237 prions, we found that BSE prions were 10- and 106-fold more resistant to inactivation, respectively. Our studies contend that any prion inactivation procedures must be validated by bioassay against the prion strain for which they are intended to be used.
"Mad cow" disease, formally known as bovine spongiform encephalopathy (BSE), belongs to a family of diseases affecting humans and a number of commercially important animal species. These diseases are not spread by bacteria or viruses, but by infectious proteins, termed "prions." Prions are known to be very difficult to inactivate, but little is known about the relative difficulty of inactivation for prions from different species. Here, we studied the inactivation of BSE prions and compared it to the inactivation of prions from humans, mice, and hamsters. We used highly sensitive, genetically engineered mouse models to detect low levels of infectivity. We then quantified the levels of inactivation for a range of treatments and calculated differences between prions from different species. We found that naturally occurring BSE prions can be up to 1 million times more difficult to inactivate than the most commonly used hamster prions. BSE prions were also 1,000 times more difficult to inactivate than a mouse prion that was thought to be a surrogate for BSE prions. This study demonstrates that prion inactivation procedures need to be validated directly against the prion strains for which they are intended to be used.
Prions are proteinaceous infectious particles that cause invariably fatal neurodegenerative diseases, including Creutzfeldt-Jakob disease (CJD) and kuru in humans, bovine spongiform encephalopathy (BSE), chronic wasting disease (CWD) in deer and elk, and scrapie in sheep and goats. The only known component of the prion is an alternatively folded isoform, denoted PrPSc, of the prion protein (PrP). The PrPSc isoform is derived from the normal, cellular PrP, denoted PrPC, through a poorly understood process. Accumulation of PrPSc in the central nervous system results in spongiform changes and death.
Distinct prions strains often exhibit different incubation periods and patterns of neuropathological lesions. Strain characteristics are generally retained upon intraspecies transmission, but may change on transmission to another species
Among the characteristics that distinguish prion strains is resistance to inactivation. The extreme resistance of prions was first noted when numerous sheep immunized against looping-ill virus developed scrapie. The inoculum had been prepared from pooled sheep brains, spinal cords, and spleens, treated with formalin and certified free of viruses
The BSE epidemic and almost 200 cases of variant CJD in the United Kingdom as well as the growing problem of CWD in the United States have raised concerns about the spread of prion infectivity. These diseases highlight the need to decontaminate effectively meat processing equipment and other instrumentation of prions. Procedures used for routine sterilization of surgical instruments do not inactivate prions, which led to the development of stringent recommendations for reprocessing instruments used on known or suspected CJD cases
We report here on the inactivation of BSE prions and a mouse-passaged BSE strain, termed 301V. The initial transmission of BSE prions to wild-type (
Mouse 301V, originally a gift from Dr. H. Fraser, was serially passaged in B6.I mice expressing the mouse PrP-B polymorphic isoform
Brain homogenates (10% w/v) were prepared in Ca2+ and Mg2+-free phosphate buffered saline (PBS; Invitrogen, Carlsbad, CA), by either repeated extrusion through successively smaller needles, via tissue disruption using stainless steel beads in a Mini-BeadBeater-8 apparatus (BioSPec, Bartlesville, OK), or with a PowerGen 125 homogenizer, as previously described
Four-millimeter segments of stainless steel suture wire (Ethicon, Cornelia, GA) were incubated in brain homogenate as previously described
Acidic SDS treatments were performed by incubating 50-µl aliquots of brain homogenate with 2× concentration of acidic SDS, for various durations (30 seconds to 18 h) and temperatures (65, 121, or 134°C). Samples were then neutralized to stop the inactivation, and either PrPSc levels were detected by Western blotting or samples were prepared for infectivity bioassay.
Treated samples were neutralized and PrPSc was determined by limited proteinase K (PK) digestion and immunoblotting as described previously
Material was eluted from wires by incubating in 10% SDS, 20 mM dithiothreitol (DTT) at 100°C for 5 min. The resulting solution was loaded onto a Minifold slot blot apparatus (Schleicher & Schuell, Florham Park, NJ) containing a PVDF membrane presoaked in ethanol.
Membranes were blocked for 30 min with 5% nonfat milk in TBST (100 mM Tris-HCl, pH 8.0; 150 mM NaCl; 0.5% Tween-20) and incubated with HRP-conjugated HuM-P monoclonal antibody diluted to 0.05 µg/ml in TBST for 1 h at room temperature. Following three 10-min washes in TBST with water rinses, membranes were developed using an enhanced chemiluminescent (ECL) detection system (Amersham, Piscataway, NJ), followed by exposure to X-ray film. Densitometry was performed with ImageJ Software (NIH, Bethesda, MD).
To directly measure infectivity of BSE prions, we performed bioassays in homozygous Tg mice expressing bovine PrP, Tg(BoPrP+/+)4092/
All mice were inoculated intracerebrally with a 30-µl aliquot of sample. Samples treated with acidic SDS were neutralized by addition of an equal volume of neutralization buffer, then diluted 10-fold in 5% BSA in PBS, to minimize nonspecific binding to the vial. For serial dilution of brain homogenate, three independent replicates of 10 ten-fold serial dilutions of 301V prions were prepared in
While incubation periods are commonly reported as the mean time from inoculation to onset of disease, analysis of this duration for samples harboring low infectivity titers requires more rigorous analysis
Specifically, to determine log reduction, we used terms for log dilution and treatment: λjk(t) = λk(t) exp(βk log dilution+δjk treatment), where the subscripts k and j denote strain and treatment, respectively. The equivalent log reduction
Western immunoblotting of the four prion strains in this study revealed distinct electrophoretic profiles. While unglycosylated PrPSc of BSE migrated to 19 kDa (
(A) Western blot of samples after limited digestion with proteinase K. Brain homogenates (0.5 mg equivalent loaded per lane) were prepared from a cow with BSE (lane 1), mouse infected with 301V (lane 2), human with sCJD (lane 3), and hamster infected with Sc237 (lane 4) prions. (B) Residual PrPSc levels of mouse 301V (filled symbols) and cattle BSE (open symbols) after treatment with 4% SDS–1% AcOH at 65°C, for 0.5 min, 1 min, 2 min, 4 min, or 8 min, as indicated. (C) Residual PrPSc levels of mouse 301V (filled bars) and cattle BSE (open bars) after treatment with 4% SDS, 1% AcOH, or water for 18 h at 65°C, as indicated. For (B) and (C), residual PrPSc levels were quantified by densitometry and compared to untreated controls. Values represent means with error bars representing standard deviations, from at least three independent measurements.
We tested the resistances of PrPSc of cattle BSE and mouse 301V to acidic SDS. Treatment with 4% SDS–1% AcOH for 0.5 to 8 min at 65°C demonstrated a clear time dependence for reduction of PrPSc levels for both strains. Although the rates of PrPSc reduction were similar, mouse 301V PrPSc was marginally more resistant to acidic SDS than cattle BSE PrPSc (
To determine more accurately the differences in inactivation of prion strains, we turned to bioassays, which are the only direct method of determining infectivity and have a much larger dynamic range than Western blots. We previously found that a two-step treatment protocol, of 4% SDS at 65°C followed by SDS at acidic pH, was the most effective at inactivating prions
|Mouse 301V in Tg2091 mice
|Cattle BSE in Tg4092 mice
|IP (95% ci)
|Sick (%)
|IP (95% ci)
|Sick (%)
|Negative control
|Positive control
|88 (81, 97)
|272 (256, 277)
|ddH2O, 18 h, 65°C
|95 (95, 99)
|298 (286, 312)
|1% AcOH, 18 h, 65°C
|102 (99, 106)
|316 (298, 368)
|4% SDS, 18 h, 65°C
|200 (151, >600)
|371 (347, 403)
|4% SDS–1% AcOH, 30 min, 65°C
|305 (244, >600)
|4% SDS–1% AcOH, 2 h, 65°C
|4% SDS–1% AcOH, 18 h, 65°C
|Untreated, 15 min, 134°C
|Untreated, 30 min, 134°C
|Untreated, 2 h, 134°C
|4% SDS–1% AcOH, 15 min, 134°C
|4% SDS–1% AcOH, 30 min, 134°C
|4% SDS–1% AcOH, 2 h, 134°C
Median incubation period (IP) in days, 95% confidence intervals (ci), and percentage of animals succumbing to prion disease (24 mice per treatment) were calculated by using Kaplan-Meier analysis.
Mouse 301V prions were only partially inactivated by acidic SDS at 65°C for 30 min: the median incubation period was significantly extended from 88 d (95% ci: 81, 97) to 305 d, but the majority of the animals still succumbed to disease. Treatment at 65°C for 18 h further decreased infectivity titers, evidenced by fewer animals (13%) becoming ill, but failed to inactivate mouse 301V prions beyond the level of detectability. A similar incidence of sick animals (14%) was seen after autoclaving at a temperature of 134°C for 15 min in the absence of acidic SDS, but an equivalent treatment with acidic SDS led to complete survival. Interestingly, treatment with SDS alone at 65°C for 18 h led to a significant reduction in mouse 301V infectivity.
Similar to 301V, cattle BSE prions were also resistant to inactivation. Treatment with acidic SDS at 65°C for 30 min produced a significant reduction in infectivity, with over 80% of the mice remaining healthy for the duration of the experiment. Increasing the treatment time increased the level of inactivation: after 18 h at 65°C with acidic SDS, no mice succumbed to disease. Autoclaving at 134°C alone (in the absence of acidic SDS) reduced BSE infectivity, but complete inactivation required 2 h. In the presence of acidic SDS, complete inactivation was achieved in 15 min at 134°C, as observed with mouse 301V.
Due to the different levels of sensitivity of
Three independent 10% brain homogenates were serially diluted 10-fold and each inoculated into 4 mice, which were then monitored for clinical symptoms of prion disease. No significant differences were observed among the three dilution series and the data were therefore combined. Incubation period is the number of days from inoculation to manifestation of clinical symptoms. Mice with intercurrent illnesses were censored at the time of euthanasia.
The incubation periods of serially diluted cattle BSE, human sCJD and hamster Sc237 prions
Two statistical methods were used to model the serially diluted mouse 301V prions: (i) a semi-parametric Cox model, which considers relative rate of disease onset, and (ii) the fully parametric Weibull model, which makes more detailed parametric assumptions of how rate of disease varies over time. For each inactivation treatment, log reductions were calculated by both methods. A plot of the log reductions determined using the Weibull model against those calculated by the Cox model demonstrates that the two methods produce very similar results (
Calculations of log reduction upon inactivation treatment, using the Weibull (abscissa) and Cox (ordinate) models, produce similar results. Serial dilutions of 301V-infected brain homogenate were modeled using the Cox and Weibull regression methods and log reduction was calculated for each treatment by both methods. Samples were treated as follows: ddH2O, 65°C, 18 h (a); 1% AcOH, 65°C, 18 h (b); 4% SDS, 65°C, 18 h (c); 4% SDS–1% AcOH at 65°C for 30 min (d), 2 h (e), and 18 h (f); autoclaved at 134°C for 15 min (g). The correlation coefficient to the line of identity is 0.99.
Values of log reduction in infectivity were calculated for each treatment and strain by comparing survival after treatment with a positive control, using the appropriate Cox model (
|301V prions
|BSE prions
|sCJD prions
|Sc237 prions
|ddH2O, 18 h, 65°C
|0.5 (0.0, 1.0)
|0.9 (0.5, 1.2)
|2.4 (1.9, 2.9)
|0.5 (0.0, 1.1)
|1% AcOH, 18 h, 65°C
|1.0 (0.5, 1.5)
|1.4 (1.0, 1.7)
|1.5 (1.2, 1.9)
|0.8 (0.4, 1.3)
|4% SDS, 18 h, 65°C
|4.3 (3.8, 5.0)
|1.7 (1.4, 2.1)
|3.3 (2.7, 4.0)
|2.5 (2.0, 3.1)
|4% SDS–1% AcOH, 30 min, 65°C
|4.8 (4.2, 5.5)
|2.9 (2.4, 3.7)
|4.9 (3.9, 6.7)
|>7.2 (6.4, >7.2)
|4% SDS–1% AcOH, 2 h, 65°C
|5.8 (5.0, 6.8)
|3.6 (2.7, 5.3)
|4.2 (3.5, 5.2)
|>7.2 (6.4, >7.2)
|4% SDS–1% AcOH, 18 h, 65°C
|6.3 (5.2, 7.8)
|>4.0 (3.1, >4.0)
|>7.2 (4.3, >7.2)
|>7.2 (6.4, >7.2)
|Untreated, 15 min, 134°C
|6.2 (5.2, 7.8)
|2.9 (2.3, 3.7)
|>7.2 (4.3, >7.2)
|7.1 (5.6, >7.2)
|Untreated, 30 min, 134°C
|>8.1 (6.5, >8.1)
|3.2 (2.5, 4.3)
|>7.2 (4.3, >7.2)
|>7.2 (6.4, >7.2)
|Untreated, 2 h, 134°C
|>8.1 (6.6, >8.1)
|>4.0 (3.1, >4.0)
|>7.2 (4.3, >7.2)
|>7.2 (6.4, >7.2)
|4% SDS–1% AcOH, 15 min, 134°C
|>8.1 (6.8, >8.1)
|>4.0 (3.1, >4.0)
|>7.2 (4.3, >7.2)
|>7.2 (6.4, >7.2)
|4% SDS–1% AcOH, 30 min, 134°C
|7.4 (5.8, >8.1)
|>4.0 (3.1, >4.0)
|>7.2 (4.4, >7.2)
|>7.2 (6.4, >7.2)
|4% SDS–1% AcOH, 2 h, 134°C
|>8.1 (6.5, >8.1)
|>4.0 (3.1, >4.0)
|>7.2 (4.4, >7.2)
|>7.2 (6.4, >7.2)
The log difference between strains for a given treatment is simply the arithmetic difference between log reductions, for a given treatment, for the two strains. However, to determine levels of significance and confidence intervals for these differences, the comparisons were stratified using individual Cox models for each strain. For treatments that led to low levels of prion inactivation (H2O or AcOH, 18 h, 65°C), mouse 301V prions and cattle BSE prions had only slightly different inactivation profiles, with a log difference of −0.4 (95% ci: −1.0, 0.2;
Similar treatments on sCJD prions showed them to be approximately 10-fold easier to inactivate than BSE prions, however many treatments led to inactivation beyond the limit of detectability for sCJD prions. With Sc237 prions the 4% SDS treatments were even more effective at resulting in complete survival of bioassay mice, and thus an inability to quantify differences between the strains. We therefore repeated the less effective 2% SDS–1% AcOH procedures on BSE prions and compared them with equivalent inactivation treatments on Sc237 and sCJD prions
Stainless-steel wires soaked in brain homogenates containing either cattle BSE or mouse 301V prions were subjected to a similar series of inactivation procedures as the brain homogenates. Wires were implanted into the brains of the respective Tg mouse lines, which were then monitored for onset of prion disease (
|Mouse 301V in Tg2091 mice
|Cattle BSE in Tg4092 mice
|IP (95% ci)
|Sick (%)
|IP (95% ci)
|Sick (%)
|Negative control
|Positive control
|113 (110, 116)
|350 (336, 357)
|ddH2O, 18 h, 65°C
|116 (109, 124)
|357 (349, 368)
|1% AcOH, 18 h, 65°C
|117 (111, 125)
|354 (336, 368)
|4% SDS, 18 h, 65°C
|127 (123, 151)
|368 (354, 398)
|4% SDS–1% AcOH, 30 min, 65°C
|267 (183, 491)
|4% SDS–1% AcOH, 2 h, 65°C
|4% SDS–1% AcOH, 18 h, 65°C
|410 (215, >600)
|Untreated, 15 min, 134°C
|161 (151, 200)
|384 (343, 456)
|Untreated, 30 min, 134°C
|438 (232, >600)
|375 (361, 386)
|Untreated, 2 h, 134°C
|420 (398, 452)
|4% SDS–1% AcOH, 15 min, 134°C
|4% SDS–1% AcOH, 30 min, 134°C
|4% SDS–1% AcOH, 2 h, 134°C
Median incubation period (IP) in days, 95% confidence intervals (ci), and percentage of animals succumbing to prion disease (24 mice per treatment) were calculated by using Kaplan-Meier analysis.
Inactivation treatments on prion-coated wires produced only moderate reductions in the level of infectivity. For example, autoclaving 301V-coated wires at 134°C for 15 min extended the median incubation period from 113 d to 161 d, and 96% of animals developed disease (
The application of survival analysis techniques to prion incubation periods provides a more accurate representation of the data. The distribution of animals succumbing to disease is rarely symmetrical, and not all animals become sick at low levels of infectivity. These factors are reflected by reporting median incubation periods and asymmetric 95% ci, which are by definition wider than standard deviation or standard error of the mean. These techniques also allow us to quantify inactivation using different Tg model systems, in an unbiased way.
We previously demonstrated that human CJD prions were significantly more resistant to inactivation than hamster Sc237 prions
Rodent-passaged prion strains are widely used in prion research. While these have been invaluable for understanding prion biology, great care must be taken in extrapolating any characteristics of these prions back to the original species and strain from which they were derived. Recommendations for the inactivation of human CJD prions were partly based on experiments on adapted strains of CJD passaged in mice
Gel electrophoresis can be used to identify the PK-resistant core of PrPSc, which can differ between strains. This technique was used to classify human prion strains based on the size of the unglycosylated, protease-resistant PrPSc fragments: type 1 results from cleavage at residue 82 and has a fragment size of 21 kDa and type 2 results from cleavage at residue 97 and has a fragment size of 19 kDa
We observed differences in the relative effectiveness of treatments on the two strains, as measured by Western blots and infectivity bioassay. In all cases, increasing the time and temperature of acidic SDS treatments led to reduced PrPSc levels and increased inactivation. For acidic SDS treatments of <8 min, Western blots of treated mouse 301V and cattle BSE prions showed similar levels of residual PrPSc (
Western blotting is a rapid and convenient way of determining the level of PrPSc, but it is limited by a dynamic range of ∼100-fold. It has long been known that the PrPSc level correlates with infectivity
The apparent discrepancies we observed between PrPSc level and infectivity for 301V and BSE prions may be due to several factors. First, the sPrPSc fraction, and its associated infectivity, may be variable between 301V and BSE. If 301V and BSE have differing amounts of rPrPSc and sPrPSc, and at least some infectivity is associated with sPrPSc, then Western blot results would not necessarily correlate with infectivity. Second, structural differences between the strains may result in different levels of inactivation. Presumably, the quaternary structure would be broken down first when inactivating prions, whereas the tertiary structure would have to be unfolded to diminish higher levels of infectivity. In such a case, mouse 301V prions may have an equally stable quaternary structure but less stable tertiary structure compared to cattle BSE prions.
Cattle or human prions passaged in rodents give rise to rodent prions. Mouse 301V prions are very resistant to inactivation
As with the results reported previously for hamster Sc237 and human sCJD prions
In conclusion, we have quantified the relative resistance to inactivation of four prion strains from different species. Cattle BSE prions appear to be the most resistant strain studied. In comparison, human sCJD prions are approximately 10-fold less resistant to inactivation by SDS at neutral or acidic pH, or by heat alone. Mouse 301V prions are 100- to 1,000-fold less resistant, and hamster Sc237 prions are up to 1,000,000-fold less resistant to inactivation. As shown by our findings, prion inactivation based on rodent-passaged prion strains may not be effective against the naturally occurring strains for which they were developed.
Kaplan-Meier survival curves of 10-fold serial dilutions of (A) cattle BSE prions bioassayed in Tg(BoPrP+/+)4092/
Variation of hazard rate across serial dilutions for the 301V prion strain. The multi-parameter spline regression (black) is closely approximated by a linear relationship (gray).
Inactivation of prion strains in brain homogenates.
Inactivation of cattle BSE prions bound to stainless steel wires.
The authors thank Ms. Hang Nguyen for expert editorial assistance, Dr. Glenn Telling for cloning the transgenic construct for the Tg(MoPrP-B) mice, and Dr. Pierre Lessard and the staff of the Hunter's Point animal facility.


text:
X marks the spot in Lakers triumph
LOS ANGELES – If Xavier Henry could hear Jordan Hill calling for the ball, so could the two players impeding his path to the rim.
The surprise player of the Lakers season faked the pass to Farmar and when the New Orleans defenders jumped, he leapt. Henry took two long strides from the free throw line and elevated. He extended the ball far behind his head with his left hand and brought down a thunderous dunk over Pelicans rookie center Jeff Withey, a college teammate at Kansas.
"He got his rookie moment," Henry joked afterward.
And the Lakers finally had a night in which the big plays went their way. After losing three of their last four games – in addition to Steve Nash to injury – the Lakers earned a 116-95 victory over New Orleans, a team that defeated the Lakers four nights earlier in the Big Easy. Henry's dunk was symbolic of the night.
"At the end of the day it's just a bucket," Henry said, "it's two points. But something to get our team going, something to get everybody's spirits up."
With Nash out for at least two weeks because of root nerve irritation, Coach Mike D'Antoni made radical changes to his starting lineup.
Out were Chris Kaman and Nick Young, and in from the tip were Jordan Hill and Wesley Johnson. Hill, who four years ago couldn't get off the bench under D'Antoni in New York, notched a career-high 21 points to go along with 11 rebounds.
Young scored 17 points to lead the bench, while Henry added 15. Pau Gasol finally broke out of a three-game slump and scored 14 points on 6-of-10 shooting.
"Maybe today," D'Antoni said. "They realized what its going to take to win."
Still, this team that sometimes feels like a walking injury report is day-to-day.
"We've been winning games (one night) and falling asleep the next," said Johnson. "So it's really just try to bounce back from this. Hopefully it carries over to tomorrow (in Denver)."
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The Design Gallery
Follow along with step-by-step beading projects and share your designs with beaders around the world—it's quick & easy!
Browse Rings
my first ring
copper wire. oh and the snake . his name is harvey .dont ask it was one of those long nites
Crystal Ring
A friend from work showed my a ring that came from the Philippines and I sort of copied the design of it...with a bit of a twist.
Components: Copper Wire and Czech Crystals
Upcycled Cocktail Rings
I hate throwing things away if I can repurpose them! These were earrings during the 80's and now they're stylish cocktail rings. :)
Components: resin cabs, adjustable ring blanks
silver skies
1940's vintage cab peyote stitched onto a sterling silver adjustable ring,with silver seed beads
Components: 1940's vintage glass cab, silver 11s and 15s seed beads, adjustable sterling silver ring
Copper Spiral
I was just goofing around with wire and created this. I seriously had no idea what the end result would be.
Copper Beauty
I love copper colors. They are so natural and pretty on their own.
better pics of rings
Hope you all can see these pics better. I really need to invest in a better camera.
All wrapped up
Thanks to Jewelry Lessons.com & Sharon for directing me to this website, this is a ring that I have been wanting to try for a couple weeks now....
Components: 4mm swarovski bicones, glass pearls, & seed beads
Cloisonne wire ring
I found this Cloisonne puffed coin bead on a bead supply site. Cloisonne beads are made using an ancient metalworking technique (also called...
Components: Cloisonne bead, copper wire
Hooked with knitting Ring
Just finished this: Knitted ring from Fine Silver wire.
Size 7.5.
Size 7.5.
Components: Fine silver
Radiance - Sardonyx Handwoven Sterling Ring
My latest gemstone ring features these beautiful Sardonyx nuggets....
Components: Sardonyx, and Sterling Silver wire
Ring Bling
I really have to quit for awhile! A few more rings...
Components: 18, 26, 28 ga wire, kyanite rounds, Hematite, lilac pearls and topaz swarovski bicones
Mini Cuff - with stones
I decided to add stones to this ring - it's a good thing to have 26 ga wire around! I also am posting the second attempt. It's interesting to see...
Components: ring#1: vintage brown wire over gunmetal core (26ga and 18ga), turquoise, Coral, ring #2: 18ga pink wire core, 26 ga magenta, pink opal and erindite swarovski crystals
A Couple of Rings
Just trying out different techniques with different types of beads.
Components: Yellow Zebra Jasper & Copper Wire ~ 18ga & 22ga Czech Rectangle & Silver Plated Wire ! 18ga & 24ga
Cuff in Miniature
The cuffs were so much fun, I decided to try it as a ring! What's really nice about this is that it is adjustable, without looking like those...
Components: 18 ga and 28 ga wire
Spinning rings
I have had plenty of training from ring making lately. Making rings wasn't my favorite, but after a lot of practicing I am feeling more comfortable...
Components: Fine silver ( Silver clay)
Ruby Heart Ring
This ring was made for one of my coworkers ....after she saw my 'Copper 'n Aqua' ring, she wanted one that represented her birthstone.
This ring...
This ring...
Components: Silver Plated Wire ~ 20ga & 22ga, Swarovski Crystals and Czech Heart Crystal
Copper 'n Aqua Ring
Just another ring...I like the way the copper shines through the crystals, giving it a whole other dimension.
Components: Faceted Aqua Czech Crystals, Copper Wire~ 12ga & 26ga
desert cloudberry
Here is my exam ring. Stripe ring with cz stone and patinated...that was what they asked.....
Myself, I do like how it turned out :) ( I am not a...
Myself, I do like how it turned out :) ( I am not a...
Blue Czech Ring
These rings are really easy to make, so I just had to make one in blue!
Components: Czech Glass Pearls, Czech Crystals and Artistic Wire-20ga & 22ga
Wired Wrapped Ring ~ (Try Saying That 3 Times Fast!)
A friend on Facebook does this type of ring and she does a beautiful job....so I had to give it a go!
Components: Artistic Wire-20ga & 22ga, Czech Faceted Rondelles and Tiny Silver Plated Beads
Double the Fun
I started with the single rings, then I thought why not try double. They are a bit more work. But fun just the same
Components: Aluminum 12g wire
Just by Accident
This was a hit at my first show last weekend. Everyone was trying them one.My best seller.I love them, they are just fun. The accident. I was...
Components: 12g aluminum wire
Crazy Tree Ring
Hmmm I love bold designs but might redo this ring to something else???
Components: Copperwire, Aquamarine f rom LB!!
African Turq. Ring
The brown wire is plated and so easily scrached :(
Components: Plated copperwire, copperwie, African Turquoise from LB!!
Rattlesnake Ring
Made this ring today!! The copperwire I have aged before I made the ring. Used the hammer a little aswell. I'm not totally happy overthe result but...
Components: Aged copperwire, (Labradorite)I guess???
Coral ring
My experiment with 4 holes rectangular coral bead
Components: Red Coral bead, non tarnish silver wire
Turquoise Inlay
My first inlay experience. Lesson learned: don't inlay (when you have to file) on a plated piece. It turned out looking pretty cool, and the base...
Components: Turquoise, Plated Pewter Ring, Loctite glue (my absolute favorite!)
Swarovski Gold Filled Wire Wrapped Ring
I just love Swarovski crystals and I love to wrap them!!! This ring pictures do not do it justice as inside all the wrapping are about 15 really...
Components: Gold filled wire wrap, Swarovski crystals, Heavy sterling silver base ring
Knotted in Love - October Bead Trends magazine
My Knotted in Love Ring is in the Oct issue of Bead Trends magazine....I'm so excited, what a thrill to actually be published!!!...
Components: Sterling Silver wire 20g
Fancy Gourmet Ring
Inspired by fancy gourmet sweets!
To bad it's not edible! Handmade with polymer clay, attached to an adjustable ring base.
To bad it's not edible! Handmade with polymer clay, attached to an adjustable ring base.
Chocolate Flower Ring
Handmade with polymer clay.
In a brown shade that looks like chocolate to me :)
In a brown shade that looks like chocolate to me :)
Sweet Rose Ring
This is an old polymer clay pendant or earring of some sort that had broken off of its setting. I glued it into the adjustable ring form. My...
Components: Polymer clay rose, Ring mount
Wrapped Blue and Gold
It's not being worn...for sale. I loved the blue/green peacock colored Swarovski crystals and had to put them onto a ring. I love the results....
Components: Galvanized Soft Gold Beads, Swarovski Crystals, Sterling Silver Base Ring, Gold Filled Wrapping Wire
Carnival Ride
Handmade of polymer clay, multicolor mixed and marbled together. Adhered to an adjustable silver ring base and finished off with a beautiful...
More Autumn
Handmade with polymer clay. Attached to an adjustable filigree ring.
Components: polymer clay, filigree ring
Zebra Filigree Ring
Handmade with polymer clay. Black and white marbled to look like a zebra :)
Attached to a black adjustable filigree ring.
Attached to a black adjustable filigree ring.
Components: polymer clay, filigree ring
Fall Inspired Flower Ring
Handmade with polymer clay.
Yellow Magnolia Ring
Handmade with polymer clay, swarovski center attached to a filigree adjustable ring.
Yellow Bold Flower Ring
Handmade with polymer clay, yellow rose ring.
- "Southwestern summer": on
"I love both pairs of these equally! I'd probably have to buy both pairs. …" read »
- "Fly away - Dragonfly Dreams": on
"This airy design proves less is more! I really love this set. It would…" read »
- "Southwestern summer": on
"Thanks ladies! Another version for a customer! …" read »
- "Southwestern summer": on
"Great colors you chose....Love them!I would definitely wear them.…" read »
- "Summer sand dollar": on
"This is really a great bracelet! Very beachy and very feminine...the asymmetry, the charm, the button…" read »
- "Summer sand dollar": on
"It's beautiful, R Fulton! I also love the beach and my eye immediately went straight to…" read »
- "Fly away - Dragonfly Dreams": on
"Sometimes less is more! I think you achieved that airy feeling. Nice design.…" read »
- "Summer sand dollar": on
"Very cute. Nice photos too!…" read »
- "Mellow Yellow": on
"Thanks everyone for the lovely comments! I think I'm going to dabble a bit in metal…" read »
- "A step back in time": on
"Thanks Moogie. The pink one is the stitch you did in your beautiful shamballa bracelet.…" read »
- "Summer sand dollar": on
"I agree with the comments above. Beachy, but still elegant and I love the leather and…" read »
- "Southwestern summer": on
"Oooh - love the colours of these earrings. The component is very cool and the…" read »
- "A Few More Bracelets": on
"Beautiful. They are all beautiful - I couldn't pick a favourite if you asked. …" read »
- "Cotton Cord Bracelets": on
"These bracelets look like so much fun to make and to wear. Love the creativity…" read »
- "Mellow Yellow": on
"Ditto to the above comments. I love the combination of the drop, the vine and…" read »
- "Day of the dead head": on
"Very cool!…" read »
- "Summer sand dollar": on
"Nicely balanced! I think the tumbled glass is so tactile.…" read »
- "Summer sand dollar": on
"Such a feminine version of a cool bracelet! Your design is very inspiring to me! I…" read »
- "Southwestern summer": on
"Beautiful earrings! They look like a wonderful size to be noticed but not weighing down the…" read »
- "Fly away - Dragonfly Dreams": on
"Very airy and summery! Love the combination of the focal with the filigree beads and the…" read »
- "Southwestern summer": on
"Lovely! I was surprised that the drops are Czech glass - they look like ruby crazy…" read »
- "Summer sand dollar": on
"What a pretty, feminine, beachy bracelet this is! The recycled glass works so well with the…" read »
- "A Few More Bracelets": on
"I love the first bracelet, the pink leather cord. It looks like we are all…" read »
- "Cotton Cord Bracelets": on
"Gorgeous! I haven't been able to get my recycled large hole beads on my cord,…" read »
- "Cotton Cord Bracelets": on
"Thanks for inspiring in a different, new way! I'm in love with the black and red…" read »
- "A Bit of Boho": on
"What type of clasp did you use? Do you have a picture of how you did…" read »
- "Cotton Cord Bracelets": on
"Okay, my favorite is the first one with the big red bead that looks silvered, gorgeous!…" read »
- "A Few More Bracelets": on
"So pretty! Love them all. I really like the patina'd coin. The cord you dyed is…" read »
- "Autumn Leaves": on
"Very cool! Love the colors in this-great use of the recycled yarn. The added charm is…" read »
- "A Few More Bracelets": on
"You're certainly on a roll! Great bracelets! I especially like the last one, the tiles really…" read »
- "Mellow Yellow": on
"Lovethe colors and the use of the vine, it/they bring everything together. Verrrry cool design!…" read »
- "A Few More Bracelets": on
"You are on a "bracelet binge" with spectacular results. Love 'em ALL! …" read »
- "Cotton Cord Bracelets": on
"Hey! It's party time! Fun, fun, fun bracelets. Love all of the colors!…" read »
- "Mellow Yellow": on
"Fooling around can sometimes result in unexpectedly unique ideas and designs. I think this fits…" read »
- "Indie Chandelier Earrings": on
"My gosh, these are so alluring that Queen Nefertiti might itching to "wake up" and wear…" read »
- "Day of the dead head": on
"A skull with a silver "Afro?" What a novel idea. It's a great design…" read »
- "LOTS OF FREELS THE LOVE LOL": on
"Each piece showcases terrific color combinations, including your choice of the metal colors and styles to…" read »
- "Soft Edges Bracelet": on
"I enjoyed seeing this gorgeous bracelet and instructions, and totally understand the comment and compliment above…" read »
- "Charmed Christmas Bracelet": on
"This bracelet is adorable, and flirty! Why not celebrate "Christmas in July?" it would spread…" read »
- "Cotton Cord Bracelets": on
"Hey, I love yours! So colorful! Love the addition of the beads! You were so focused…" read »


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Town Square
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COMMENTS ON STORIESTornado warning canceled
The National Weather Service has canceled its tornado warning for Contra Costa County, which was issued after a funnel cloud...Original post made on Jan. 24, 2010
Last comment by CDSI Research, on Jan 25, 2010 at 7:04 am | 2 comments | 1266 Views
COMMENTS ON STORIESSRVUSD Board installs new member, approves new project and policies
The five person San Ramon Valley Unified School District Board of Education was installed for a new term Tuesday. The board...Original post made on Dec. 15, 2010
There are no comments yet. 1266 Views
COMMENTS ON STORIESHelp fight birth defects at bingo fundraiser
Enjoy a day of bingo, food and friendship at the Nayeli Faith Foundation Bingo Fundraiser on Sunday, Oct. 7 at the Alamo...Original post made on Oct. 5, 2012
Last comment by Liz, on Oct 5, 2012 at 9:04 pm | 1 comment | 1266 Views
COMMENTS ON STORIESElderly man critically injured in hit-and-run collision
Police are attempting to track down a suspect in a hit-and-run collision in San Ramon last Friday that critically injured...Original post made on Nov. 16, 2012
Last comment by Jana, on Nov 16, 2012 at 8:52 am | 1 comment | 1266 Views
COMMENTS ON STORIESWhat have we learned (so far) from Boston?
"The nation can now catch its collective breath, in the aftermath of the Boston Marathon bombing and speedy, live capture...Original post made on Apr. 25, 2013
Last comment by Louise, on Apr 25, 2013 at 2:36 pm | 1 comment | 1266 Views
STATE, NATIONAL, INTERNATIONALSmart Meters can be removed
Dear Jessica, this just in! California utilities commission to allow customers to opt-out of smart meters 03/10/2011 By...Original post made by Hal, CDSI Research Fellowship on Mar. 10, 2011
There are no comments yet. 1265 Views
AROUND TOWNAn R-7A mandate in Alamo?
Dear Editor, Polling of Alamo neighborhoods by news service researchers indicated 67% of eligible voters submitted Measure...Original post made by [removed] on Jun. 16, 2011
There are no comments yet. 1265 Views
AROUND TOWNAttend the Danville Fine Arts Faire
Enjoy 200 fine art and contemporary craft displays, Italian street painting, wonderful entertainment and more! The Danville...Original post made on Jun. 21, 2013
Last comment by John, on Jun 21, 2013 at 7:08 am | 1 comment | 1265 Views
COMMENTS ON STORIESPiedmont Lumber goes up in flames
Heavy black smoke from a four-alarm fire in Walnut Creek could be seen in the San Ramon Valley as its firefighters were among...Original post made on Mar. 14, 2010
Last comment by jrm, on Mar 16, 2010 at 5:52 pm | 2 comments | 1265 Views
COMMENTS ON STORIESCounty Sherriff's Office looking for missing Diablo man
The Contra Costa County Sheriff's Office is searching for an 84-year-old man who left his residence on Saturday afternoon...Original post made on Aug. 30, 2010
Last comment by Jim, on Aug 31, 2010 at 8:42 am | 3 comments | 1265 Views
COMMENTS ON STORIESHave you seen Brad Pitt?
Rumors have been spreading about Brad Pitt sightings in Diablo and Danville. This editor literally jumped at the news and...Original post made on Oct. 4, 2010
Last comment by Dave, on Oct 4, 2010 at 12:37 pm | 5 comments | 1265 Views
AROUND TOWNViolins will perform with Diablo Symphony Feb. 12
On Feb. 12, violinist Karen Shinozaki will perform with the Diablo Symphony in Walnut Creek under the baton of guest conductor...Original post made on Feb. 10, 2012
Last comment by Eva Langfeldt, on Feb 10, 2012 at 8:08 am | 1 comment | 1264 Views
AROUND TOWNSRV students reflect on volunteerism in America
As part of its annual Sept. 11 remembrance, Exchange Club of the San Ramon Valley hosted an essay contest for students throughout...Original post made on Oct. 24, 2012
Last comment by kstepper, on Oct 24, 2012 at 1:07 pm | 1 comment | 1264 Views
COMMENTS ON STORIESAll-you-can-eat claws for paws
Join Tri-Valley Animal Rescue on Saturday, Feb. 4, from 6-10 p.m. for a Claws for Paws fundraising event with all-you-can-eat...Original post made on Jan. 18, 2012
Last comment by karl the krab, on Jan 20, 2012 at 6:01 am | 3 comments | 1264 Views
COMMENTS ON STORIESWednesday is another Spare the Air day
Another Winter Spare the Air alert banning wood-burning has been issued for the Bay Area on Wednesday. It is the eighth time...Original post made on Jan. 23, 2013
Last comment by the dogfather, on Jan 23, 2013 at 1:55 pm | 5 comments | 1264 Views
COMMENTS ON STORIESOccupiers march to Castlewood Country Club
Between 300 and 350 people turned out for last weekend's Occupy Pleasanton event to protest the lockout of workers from Castlewood...Original post made on Mar. 1, 2012
Last comment by Rick, on Mar 1, 2012 at 8:25 am | 1 comment | 1263 Views
COMMENTS ON STORIESGrand jury returns indictment against state senator, 28 others
State Sen. Leland Yee, Raymond "Shrimp Boy" Chow and 27 other defendants have been indicted by a federal grand jury in San...Original post made on Apr. 7, 2014
Last comment by Derek, on Apr 8, 2014 at 4:45 pm | 6 comments | 1263 Views
COMMENTS ON STORIESBaker's 'Cancer Survivors Day' resolution passes off Assembly floor
Assemblywoman Catharine Baker (R-San Ramon) announced Tuesday that her bill to designate Sunday, June 7, 2015 as California...Original post made on Jun. 3, 2015
Last comment by American, on Jun 3, 2015 at 12:42 pm | 2 comments | 1263 Views
AROUND TOWNTuning into the Tri-Valley
The Tri-Valley's community television station may have experienced some bumps in the road, but new programming and leadership...Original post made on Oct. 16, 2009
Last comment by Melissa Tench-Stevens, on Oct 21, 2009 at 7:05 pm | 3 comments | 1262 Views
AROUND TOWN'Habitat' gets $100,000 matching grant for car donation program
Habitat for Humanity East Bay has been awarded a $100,000 matching grant from Habitat for Humanity International's vehicle...Original post made on Dec. 23, 2009
Last comment by Alena, on Dec 31, 2009 at 12:16 am | 3 comments | 1262 Views
Republican, Democrat, Libertarian, Green
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Looking for financial tips? Some experts recommend free or low-cost services offered
through your employer, an individual planner or
online tool.
Entrepreneurial Q&A: If you could recommend that any developer you hire take just one course/class, what subject would it be in and why?
Fiat and Chrysler may be growing closer in the boardroom, but the
companies are showing different convertibles at
the NY Auto Show.
Most cars and trucks get recalled at some point. Recalls address safety problems, even if they are minor.
Hyundai Motor Co. said it will guarantee the trade-in price of new cars sold in the U.S. starting May 1.
A South African did a feat no other person from his country has
completed-- Sibusiso Vilane is the first South African to climb Mount
Summer is upon us which means it's time to head to the ocean. When it comes to beach vacations, why not go to one of the best...Virginia Beach?
Mama Sophia may be the author of a new bestselling autobiography, but there is much more to her story.
The Broadway cast of "Fela" introduced Afrobeat superstar Fela
Anikulapo-Kuti to hundreds of thousands of new American fans. Now the
show's stars are facing their toughest critics yet: Nigerians in this
megacity who know his lyrics by heart and consider him a national icon.
With shoppers flocking back to showrooms, auto dealers are making money
again and have begun sprucing up reception areas and hiring back
New-car buyers won't like the sound of this, but the days of bargaining may be coming to an end.
Samuel L. Jackson will make his Broadway debut this fall as the Rev. Martin Luther King Jr.


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JPMorgan & Wells Fargo Release Earnings Tomorrow
Oct. 11 (Bloomberg) -- Bloomberg News' Christine Harper discusses what to expect from bank earnings. She speaks with Matt Miller on Bloomberg Television's "Bloomberg Rewind." (Source: Bloomberg)
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Cinderella - Royal Edition [DVD]
Get £1 Off Amazon Video*
|£4.41 & FREE Delivery in the UK on orders over £20. Details
Fulfilment by Amazon (FBA) is a service Amazon offers sellers that lets them store their products in Amazon's warehouses, and Amazon directly does the picking, packing, shipping and customer service on these items. Something Amazon hopes you'll especially enjoy: FBA items are eligible for and for Amazon Prime just as if they were Amazon items.
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Product Description
Walt Disney's Cinderella, based on the world's greatest fairy tale, has captivated audiences for generations with its spellbinding story, memorable music, spectacular animation and unforgettable characters. Experience the magic in this Royal Edition, with Disney Enhanced Home Theatre Mix and music videos, and you too will believe that dreams really do come true.
Bonus Features:
- "A Dream is a Wish Your Heart Makes" - Music Video performed by Disney Channel "Circle of Stars"
- "Every Girl Can Be a Princess" - Music Video Featuring Disney's Animated Princesses
From Amazon.co.uk
Worry not, Disney fans--this special edition DVD of the beloved Cinderella won't turn into a pumpkin at the strike of midnight. One of the most enduring animated films of all time, the Disney-fied adaptation of the gory Brothers Grimm fairy tale became a classic in its own right, thanks to some memorable tunes (including "A Dream Is a Wish Your Heart Makes," "Bibbidi-Bobbidi-Boo," and the title song) and some endearingly cute comic relief.
We all know the story--the wicked stepmother and stepsisters simply won't have it, this uppity Cinderella thinking she's going to a ball designed to find the handsome prince an appropriate sweetheart, but perseverance, animal buddies, and a well-timed entrance by a fairy godmother make sure things turn out all right. There are a few striking sequences of pure animation--for example, Cinderella is reflected in bubbles drifting through the air--and the design is rich and evocative throughout. It's a simple story padded here agreeably with comic business, particularly Cinderella's rodent pals (dressed up conspicuously like the dwarf sidekicks of another famous Disney heroine) and their misadventures with a wretched cat named Lucifer. There's also much harrumphing and exposition spouting by the King and the Grand Duke. It's a much simpler and more graceful work than the more frenetically paced animated films of today, which makes it simultaneously quaint and highly gratifying. --David Kronke, Amazon.com --This text refers to an out of print or unavailable edition of this title.
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Customer Reviews
Top Customer Reviews
As for the movie though it's exactly the same, as it should be in my opinion. I'd recommend this classic but if you already have an older copy I wouldn't worry about getting this one. If you're new to Cinderella or looking for a nice gift idea for children then this is a brilliant movie to invest in.
A really lovely, magical classic - not to be missed.
The tale is familiar. A beautiful girl is abused by her stepmother and stepsisters and forced to work as their slave. When a ball is given for all the eligible maidens in the kingdom, Cinderella dreams of going too, only to have her hopes dashed at the last minute. With a gift of magic, she is able to go, but only until the stroke of midnight.
The story is so well known, if fact, that any story about an underdog coming from behind and winning is called a Cinderella story. Disney's job here was to make the story entertaining for 75 minutes. The solution here was creating the animals. Mice Jaq and Gus and their constant struggle against the evil cat Lucifer is funny and tying this sub-plot to the ultimate outcome is brilliant. The other standout character is the stepmother who is frighteningly evil with no super powers.
As with any Disney movie, this one also has great songs. Arguably the best known is "Bibbidi-Bobbidi-Boo." Right behind it is "A Dream is a Wish Your Heart Makes." My favorite, however, is the often overlooked love song "So This is Love."
While not the best release in the Platinum series, it still stacks up well. The movie has been restored, and it looks great to me. I'm not a nitpicker, but I can't find any problems with the full frame picture, the original aspect ratio. There is an enhanced 5.1 home theater mix, which doesn't seem quite as impressive as some other releases. It seems more like the sound coming equally from all speakers. Of course, given the age of the film, there probably isn't much more they could do.Read more ›
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It's worth noting that as 'old' Disney, there are some fairly dark moments in this film. Having said that, our 4 year old loves the story and the film.Published 22 days ago by Hannah
Favourite Disney film of all time! Such a classic & so magical!Published 1 month ago by alan warsop
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The Disturbing Movies Test
Their result for The Disturbing Movies Test ...
Completely Disturbed
100% Knowledge of Disturbing Cinema.
Their Analysis (Vertical line = Average)
They scored 100% on knowledge, higher than 78% of your peers.
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You're probably not into really disturbing films; you may have seen a couple, but you don't go out of your way to view them. You would prefer to spend your free time gazing at the non-threaten... Read more
Fairly Disturbed
It appears you've seen a fair amount of disturbing cinema. In fact, you might even go out of your way to see more. When someone describes a film that disturbed them, you probably ask for the name of... Read more
Damn Disturbed
You thoroughly enjoy the disturbing cinema; images and ideas that "get under your skin" have an appeal to you. You've watched the majority of films on this test, possibly even multiple times, and you... Read more
Completely Disturbed
With your knowledge (and, one assumes, love of) disturbing cinema, you should have been the one to write this test. Not only have you watched just about every film on this test, but you can probably ... Read more
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Awesome: 0%
Worth A Look: 10%
Average: 10%
Pretty Bad: 20%
Total Crap: 60%
1 review, 4 user ratings
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"Two awful flicks jammed together. See if you can spot the seams!"
USA 01-Apr-1974 (R) DVD: 24-Feb-2004UK N/A (18)Australia 01-Apr-1974
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When Christmas comes the world lights up, literally. Those decorations you've put on the house won't just brighten up the neighbourhood, but they could be sending a message to aliens that a festive season is underway.
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Homeowners don't just decorate their homes with holiday lights and other festive signs of the season, but many enshrine their shrubbery and trees with the twinkling lights. This may all be well and good, but for wildlife it's another story.
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Mark Weitzman
Simple strings of lights dot the neighborhoods while traditional holiday lighting illuminates the shopping centers and plazas.
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Christmas lights show at Capilano Suspension Bridge in North Vancouver.
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The "Star Tribune" reports NFL commissioner Roger Goodell will be at the state capitol today to lobby for a new stadium for the Vikings. According to the report, Goodell will meet with Republican and DFL leaders today. Earlier this week, a House committee voted down the latest plan for a new stadium near the Metrodome
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The Peanuts Character Test
All possible test results
Winner of various fuss-budget awards and permanently crabby, Lucille Van Pelt is the older sister of Linus and Rerun. When she isn't giving Charlie Brown questionable advice on the nature of the world... Read more
Peppermint Patty
Despite sandals being generally unsuitable for running, Peppermint Patty is a world-class sports player and has gone toe-to-toe with Snoopy at both baseball and football. She spends most of her lesson... Read more
Perhaps the world's most uncoordinated bird, Woodstock is Snoopy's best friend, activity partner and occasional secretary. With his erratic flight patterns, he has trouble avoiding anything roughly be... Read more
Joe Cool...you are the world's favourite beagle! Snoopy is owned by Charlie Brown (although this relationship often works the other way round) and spends his days sleeping on top of the kennel - that ... Read more
Marcie is Peppermint Patty's best friend, and secretly loves Charlie Brown. She is always willing to help Patty through class and with homework, and plays on her sports teams even though she would rat... Read more
The only other character who can match Linus for Old Testament knowledge, Franklin is probably the most stable and together of all the gang. He goes to the same school as Peppermint Patty and Marcie a... Read more
Charlie Brown
What can we say about good ol' Charlie Brown that you don't already know? From sarcastic beagles to kite-eating trees to little red-haired girls, nothing seems to ever go quite right for our hero. Wel... Read more
With Linus and Lucy for older siblings, Rurun has grown up slightly cynical and wary of the outside world - and who can blame him? He's too short to play baseball with the rest of the gang, but is sur... Read more
A prodigy from his very first appearance, Schroeder idolises Beethoven and spends most of his free time at the keyboard of his piano. He plays backstop for Charlie Brown's baseball team, but his heart... Read more
With the outside world being such a big and scary place, everyone needs a source of comfort and security. For Linus this is his blanket - even though Lucy and his grandmother try and break him of the ... Read more
Sally is Charlie Brown's little sister and has an unrequited love for Linus. Alternately amazed and terrified by the world of school, she gets by on pure stubbornness. If you're feeling a little down ... Read more
Perhaps the most aptly named character, Pigpen is shrouded in a permanent cloud of dust and dirt. It's not his fault, he just attracts the stuff and ends up contaminating everyone nearby. There's plen... Read more
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Talk to a live representative about how to go back to college!
A career in wellness is about contributing to others' overall health. There are a variety of careers that can be pursued with this major as you can practice your skills with a physical therapist, at a wellness center, on cruises or even start your own practice.
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Most Popular Titles With Andrew Fensom
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- IMDb Rating
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1. Star Trek Beyond (2016)
PG-13 | 122 min | Action, Adventure, Sci-Fi
The USS Enterprise crew explores the furthest reaches of uncharted space, where they encounter a new ruthless enemy who puts them and everything the Federation stands for to the test.
Writers: Simon Pegg, Doug Jung, Gene Roddenberry | Director: Justin Lin | Stars: Chris Pine, Zachary Quinto, Karl Urban, Zoe Saldana
Votes: 24,931 | Gross: $59.60M
2. Batman v Superman: Dawn of Justice (2016)
PG-13 | 151 min | Action, Adventure, Sci-Fi
Fearing that the actions of Superman are left unchecked, Batman takes on the Man of Steel, while the world wrestles with what kind of a hero it really needs.
Writers: Chris Terrio, David S. Goyer, Bob Kane, Bill Finger, Jerry Siegel, Joe Shuster | Director: Zack Snyder | Stars: Ben Affleck, Henry Cavill, Amy Adams, Jesse Eisenberg
Votes: 350,324 | Gross: $330.25M
3. Captain America: Civil War (2016)
PG-13 | 147 min | Action, Adventure, Sci-Fi
Political interference in the Avengers' activities causes a rift between former allies Captain America and Iron Man.
Writers: Christopher Markus, Stephen McFeely, Joe Simon, Jack Kirby | Directors: Anthony Russo, Joe Russo | Stars: Chris Evans, Robert Downey Jr., Scarlett Johansson, Sebastian Stan
Votes: 261,784 | Gross: $406.57M
4. The Jungle Book (2016)
PG | 106 min | Adventure, Drama, Family
After a threat from the tiger Shere Khan forces him to flee the jungle, a man-cub named Mowgli embarks on a journey of self discovery with the help of panther, Bagheera, and free spirited bear, Baloo.
Writers: Justin Marks, Rudyard Kipling | Director: Jon Favreau | Stars: Neel Sethi, Bill Murray, Ben Kingsley, Idris Elba
Votes: 95,471 | Gross: $360.85M
5. The Brothers Grimsby (2016)
R | 83 min | Action, Comedy
A new assignment forces a top spy to team up with his football hooligan brother.
Writers: Sacha Baron Cohen, Phil Johnston, Peter Baynham | Director: Louis Leterrier | Stars: Sacha Baron Cohen, Mark Strong, Rebel Wilson, Freddie Crowder
Votes: 37,841 | Gross: $6.86M
6. Ant-Man (2015)
PG-13 | 117 min | Action, Adventure, Comedy
Armed with a super-suit with the astonishing ability to shrink in scale but increase in strength, cat burglar Scott Lang must embrace his inner hero and help his mentor, Dr. Hank Pym, plan and pull off a heist that will save the world.
Writers: Edgar Wright, Joe Cornish, Adam McKay, Paul Rudd, Stan Lee, Larry Lieber, Jack Kirby | Director: Peyton Reed | Stars: Paul Rudd, Michael Douglas, Corey Stoll, Evangeline Lilly
Votes: 309,062 | Gross: $180.19M
7. Edge of Tomorrow (2014)
PG-13 | 113 min | Action, Adventure, Sci-Fi
A soldier fighting aliens gets to relive the same day over and over again, the day restarting every time he dies.
Writers: Christopher McQuarrie, Jez Butterworth, John-Henry Butterworth, Hiroshi Sakurazaka | Director: Doug Liman | Stars: Tom Cruise, Emily Blunt, Bill Paxton, Brendan Gleeson
Votes: 429,224 | Gross: $100.19M
8. Transformers: Age of Extinction (2014)
PG-13 | 165 min | Action, Adventure, Sci-Fi
Autobots must escape sight from a bounty hunter who has taken control of the human serendipity: Unexpectedly, Optimus Prime and his remaining gang turn to a mechanic, his daughter, and her back street racing boyfriend for help.
Writer: Ehren Kruger | Director: Michael Bay | Stars: Mark Wahlberg, Nicola Peltz, Jack Reynor, Stanley Tucci
Votes: 241,542 | Gross: $245.43M
9. John Carter (2012)
PG-13 | 132 min | Action, Adventure, Sci-Fi
Transported to Barsoom, a Civil War vet discovers a barren planet seemingly inhabited by 12-foot tall barbarians. Finding himself prisoner of these creatures, he escapes, only to encounter Woola and a princess in desperate need of a savior.
Writers: Andrew Stanton, Mark Andrews, Michael Chabon, Edgar Rice Burroughs | Director: Andrew Stanton | Stars: Taylor Kitsch, Lynn Collins, Willem Dafoe, Samantha Morton
Votes: 211,696 | Gross: $73.06M
10. World War Z (2013)
PG-13 | 116 min | Action, Adventure, Horror
Former United Nations employee Gerry Lane traverses the world in a race against time to stop the Zombie pandemic that is toppling armies and governments, and threatening to destroy humanity itself.
Writers: Matthew Michael Carnahan, Drew Goddard, Damon Lindelof, J. Michael Straczynski, Max Brooks | Director: Marc Forster | Stars: Brad Pitt, Mireille Enos, Daniella Kertesz, James Badge Dale
Votes: 463,084 | Gross: $202.35M
11. Skyfall (2012)
PG-13 | 143 min | Action, Adventure, Thriller
Bond's loyalty to M is tested when her past comes back to haunt her. Whilst MI6 comes under attack, 007 must track down and destroy the threat, no matter how personal the cost.
Writers: Neal Purvis, Robert Wade, John Logan | Director: Sam Mendes | Stars: Daniel Craig, Javier Bardem, Naomie Harris, Judi Dench
Votes: 520,282 | Gross: $304.36M
12. 300: Rise of an Empire (2014)
R | 102 min | Action, Drama, Fantasy
Greek general Themistokles leads the charge against invading Persian forces led by mortal-turned-god Xerxes and Artemisia, vengeful commander of the Persian navy.
Writers: Zack Snyder, Kurt Johnstad, Frank Miller | Director: Noam Murro | Stars: Sullivan Stapleton, Eva Green, Lena Headey, Hans Matheson
Votes: 224,423 | Gross: $106.37M
13. Clash of the Titans (2010)
PG-13 | 106 min | Action, Adventure, Fantasy
Perseus demigod, son of Zeus, battles the minions of the underworld to stop them from conquering heaven and earth.
Writers: Travis Beacham, Phil Hay, Matt Manfredi, Beverley Cross | Director: Louis Leterrier | Stars: Sam Worthington, Liam Neeson, Ralph Fiennes, Jason Flemyng
Votes: 229,151 | Gross: $163.19M
14. Prince of Persia: The Sands of Time (2010)
PG-13 | 116 min | Action, Adventure, Fantasy
A young fugitive prince and princess must stop a villain who unknowingly threatens to destroy the world with a special dagger that enables the magic sand inside to reverse time.
Writers: Boaz Yakin, Doug Miro, Carlo Bernard, Jordan Mechner | Director: Mike Newell | Stars: Jake Gyllenhaal, Gemma Arterton, Ben Kingsley, Alfred Molina
Votes: 221,680 | Gross: $90.76M
15. Strange Magic (2015)
PG | 99 min | Animation, Family, Fantasy
Goblins, elves, fairies and imps, and their misadventures sparked by the battle over a powerful potion.
Writers: David Berenbaum, Irene Mecchi, Gary Rydstrom, George Lucas | Director: Gary Rydstrom | Stars: Evan Rachel Wood, Elijah Kelley, Kristin Chenoweth, Maya Rudolph
Votes: 6,543 | Gross: $12.43M
16. Leap Year (2010)
PG | 100 min | Comedy, Romance
Anna Brady plans to travel to Dublin, Ireland to propose marriage to her boyfriend Jeremy on Leap Day, because, according to Irish tradition, a man who receives a marriage proposal on a leap day must accept it.
Writers: Deborah Kaplan, Harry Elfont | Director: Anand Tucker | Stars: Amy Adams, Matthew Goode, Adam Scott, John Lithgow
Votes: 71,017 | Gross: $0.01M
17. Battle Los Angeles (2011)
PG-13 | 116 min | Action, Sci-Fi
A squadron of U.S. Marines becomes the last line of defense against a global invasion.
Writer: Christopher Bertolini | Director: Jonathan Liebesman | Stars: Aaron Eckhart, Michelle Rodriguez, Bridget Moynahan, Ramon Rodriguez
Votes: 154,692 | Gross: $83.55M
18. The Bad Education Movie (2015)
90 min | Comedy
Mr Wickers and his class go on one final school trip after they finish their GCSEs.
Creators: Jack Whitehall, Ben Cavey | Director: Elliot Hegarty | Stars: Jack Whitehall, Charlie Wernham, Nikki Runeckles, Weruche Opia
Votes: 3,106
19. Marmaduke (2010)
PG | 87 min | Comedy, Family
A suburban family moves to a new neighborhood with their large yet lovable Great Dane, who has a tendency to wreak havoc in his own oblivious way.
Writers: Tim Rasmussen, Vince Di Meglio, Brad Anderson, Phil Leeming | Director: Tom Dey | Stars: Owen Wilson, Judy Greer, Lee Pace, Emma Stone
Votes: 11,224 | Gross: $33.64M
20. Geostorm (2017)
Action, Sci-Fi, Thriller | Post-production
As a man heads into space to prevent climate-controlling satellites from creating a storm of epic proportions, his brother discovers a plot to assassinate the president.
Writers: Dean Devlin, Paul Guyot | Director: Dean Devlin | Stars: Katheryn Winnick, Gerard Butler, Abbie Cornish, Andy Garcia
21. The Hunt for Gollum (2009)
Unrated | 38 min | Short, Action, Adventure
Strider must hunt down Gollum to keep the Ring secret.
Writers: J.R.R. Tolkien, Chris Bouchard, Sabina Sattar, Julianne Honey-Mennal, Stuart Price, Lewis Albrow | Director: Chris Bouchard | Stars: Adrian Webster, Arin Alldridge, Patrick O'Connor, Rita Ramnani
Votes: 4,579
22. The Christmas Miracle of Jonathan Toomey (2007)
91 min | Family
When a broken hearted boy loses the treasured wooden nativity set that links him to his dead father, his worried mother persuades a lonely ill-tempered woodcarver to create a replacement, ... See full summary »
Writers: Bill Clark, Susan Wojciechowski | Director: Bill Clark | Stars: Tom Berenger, Sarah Wildor, Thomas Briggs, Joely Richardson
Votes: 695
23. Wavelengths (I) (2011)
12 min | Short, Drama
Writers: Oliver Stevens, Matt Zeqiri | Directors: Matt Houghton, James Spinney | Stars: James Smith, Kate Anthony
24. Ex Cathedra (2009)
135 min | Drama, Thriller
As Kevin's life starts to crumble around him a new one opens up before him full of exotic drugs, double dealings, mysterious girls and fallen angels.
Creator: Daniel Castles | Director: Liam Andrew Wright | Stars: Gabrielle Amies, Paul Bamgbose, Glen Cardno, Donnie Castillo
Votes: 11