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Autism spectrum disorders (ASD) are neurodevelopmental diseases that affect an alarming number of individuals. The etiological basis of ASD is unclear, and evidence suggests it involves both genetic and environmental factors. There are many reports of cytokine imbalances in ASD. These imbalances could have a pathogenic role, or they may be markers of underlying genetic and environmental influences. Cytokines act primarily as mediators of immunological activity, but they also have significant interactions with the nervous system. They participate in normal neural development and function, and inappropriate activity can have a variety of neurological implications. It is therefore possible that cytokine dysregulation contributes directly to neural dysfunction in ASD. Further, cytokine profiles change dramatically in the face of infection, disease, and toxic exposures. Therefore, imbalances may represent an immune response to environmental contributors to ASD. The following review is presented in two main parts. First, we discuss select cytokines implicated in ASD, including IL-1Β, IL-6, IL-4, IFN-γ, and TGF-Β, and focus on their role in the nervous system. Second, we explore several neurotoxic environmental factors that may be involved in the disorders, and focus on their immunological impacts. This review represents an emerging model that recognizes the importance of both genetic and environmental factors in ASD etiology. We propose that the immune system provides critical clues regarding the nature of the gene by environment interactions that underlie ASD pathophysiology.
Autism spectrum disorders (ASD) are clinically variable neurodevelopmental disorders that arise in early childhood. ASD is diagnosed in 1 out of every 88 children in the United States, and is characterized by stereotypic behaviors and impaired language and communication (American Psychiatric Association 1994; Lord, Rutter et al. 1994; Prevention 2009; CDC 2012). The biological basis of ASD remains largely elusive. Postmortem investigations have demonstrated abnormal brain growth, including neuronal overgrowth in some regions (Courchesne, Mouton et al. 2011), and undergrowth in others (Schumann and Amaral 2006). Altered ratios of excitatory to inhibitory signaling in the brain may also contribute to ASD (Fatemi, Halt et al. 2002; Rubenstein and Merzenich 2003; Fatemi, Reutiman et al. 2010; Yizhar, Fenno et al. 2011). Inheritance analyses have demonstrated a large genetic component, although a recent twin study suggests that non-genetic environmental factors also make a substantial contribution (Hallmayer, Cleveland et al. 2011; Ronald and Hoekstra 2011). A multitude of genes have been implicated in autism, but only a few cases can be traced to specific rare genetic variants, and many of the implicated genes are also found in typically developing populations (State and Levitt 2011). Collectively, these findings suggest that autism results from complex interactions between multiple susceptibility genes and environmental factors.
A growing body of evidence implicates immunological disturbances in ASD. Several of the genes linked to ASD have critical roles in immune signaling, activation, and regulation (Torres, Maciulis et al. 2002; Campbell, Sutcliffe et al. 2006; Lee, Zachary et al. 2006; Torres, Sweeten et al. 2006; Varga, Pastore et al. 2009; Orlova and Crino 2010). Individuals with autism and their family members (primarily mothers) demonstrate increased autoimmunity, altered cellular immunity, and skewed expression of soluble mediators like cytokines (Vargas, Nascimbene et al. 2005; Braunschweig, Ashwood et al. 2008; Li, Chauhan et al. 2009; Morgan, Chana et al. 2010; Goines, Haapanen et al. 2011; Onore, Careaga et al. 2011). Cytokine abnormalities in ASD are an important clue for researchers as they might result from genetic and environmental factors, and may contribute directly to neurological dysfunction. The following considers the neurological significance of cytokine dysregulation in ASD, and how environmental factors can modulate the cytokine response.
The immune system and the nervous system interact extensively. It is therefore not surprising that immune dysfunction is often noted in neurological disorders. Immune mediators known as cytokines are key facilitators of cross-systemic communication. Cytokines are proteins that control the nature, duration, and intensity of an immune response. They are highly pleiotropic, and can act in an autocrine, paracrine, and/or endocrine fashions. Immune cells, including dendritic cells, macrophages, neutrophils, T cells, and B cells, are the primary source of cytokines; though many additional cell types, including neurons, produce and respond to them. Cytokines share structural similarities and signaling pathways with neurotrophins and neurologically relevant growth factors. In many ways, cytokines represent a common language between the immune system and the nervous system.
Cytokines influence both the development and function of the nervous system. Their significance varies based on the timing, duration, and intensity of the neuro-immune interaction. For example, cytokines impact the developing brain differently than the adult brain; and may be beneficial at one concentration while harmful at another. Cytokines are involved in normal aspects of neurodevelopment, including progenitor cell differentiation, cellular localization/migration within the nervous system, and synaptic network formation (Deverman and Patterson 2009). During infection and illness, cytokines mediate neurological changes associated with fever and sickness behavior by signaling directly to the hypothalamus (Dantzer 2001; Skurlova, Stofkova et al. 2006). Emerging evidence also implicates cytokines in higher order neurological functions, including cognition and memory (McAfoose and Baune 2009; Derecki, Cardani et al. 2010). Imbalanced cytokine production, signaling, and/or regulation can therefore have a wide range of neurological consequences.
Aberrant expression of cytokines and their signaling intermediaries is often noted in ASD (Table 1). This is observed in the brain (Vargas, Nascimbene et al. 2005; Grigorenko, Han et al. 2008; Voineagu, Wang et al. 2011; Ziats and Rennert 2011) peripheral blood (Molloy, Morrow et al. 2006; Ashwood, Krakowiak et al. 2011) and the gastrointestinal tract (DeFelice, Ruchelli et al. 2003; Ashwood, Anthony et al. 2004). Cytokine imbalances during development and/or throughout life could impact neural activity and mediate behavioral aspects of the disorder. The following considers the significance of several cytokines linked to ASD.
IL-1Β is an inflammatory cytokine expressed very early in immune responses (Jiang, Tian et al. 1997). In tissue, IL-1Β propagates inflammation by activating local immune cells and the vascular endothelium. Systemically, IL-1Β stimulates IL-6 production and eventually an acute phase response in the liver. Systemic IL-1Β can cross the blood brain barrier (Banks, Ortiz et al. 1991) and stimulate its own expression in the hypothalamus, which leads to neuroendocrine changes associated with fever and sickness behavior (Dantzer 2001; Skurlova, Stofkova et al. 2006). IL-1Β receptors are structurally related to toll-like receptors (TLRs), and signaling is achieved through NF-κB and MAP kinase (MAPK) signaling cascades (O'Neill 2000). IL-1Β belongs to an evolutionarily conserved family of proteins that function beyond immunity (Barksby, Lea et al. 2007). It shares structural homology with fibroblast growth factors (Zhang, Cousens et al. 1991), which are critical in embryonic neurodevelopment, and are implicated in autism and schizophrenia (Tabares-Seisdedos and Rubenstein 2009; Stevens, Smith et al. 2010).
Genes for IL-1Β, its receptor, and its receptor-associated proteins are associated with intellectual disability, schizophrenia, and autism (Katila, Hanninen et al. 1999; Piton, Michaud et al. 2008; Handley, Lian et al. 2010). Children and adults with autism have increased plasma IL-1Β and skewed cellular IL-1Β responses following stimulation (Ashwood, Krakowiak et al. 2011; Suzuki, Matsuzaki et al. 2011). Compared to controls, monocytes from children with ASD produce excessive IL-1Β following LPS exposure (Jyonouchi, Sun et al. 2001; Enstrom, Onore et al. 2010), and lower levels following exposure to TLR 9 agonists (Enstrom, Onore et al. 2010). The IL-1 antagonist, IL-1ra, is also increased among ASD subjects (Suzuki, Matsuzaki et al. 2011). IL-1ra reduces inflammation by competing for the IL-1Β receptor, and increased levels may represent an attempt to counteract inflammation in ASD. Postmortem brains from ASD subjects had normal IL-1Β levels (Li, Chauhan et al. 2009), but given that peripheral IL-1Β can enter the brain (Banks, Ortiz et al. 1991), increased systemic levels could directly impact neurological processes.
IL-1Β disruption can have a variety of neurological consequences relevant to autism. The cytokine and its receptors are found throughout the nervous system during critical developmental periods (Giulian, Young et al. 1988). IL-1Β induces neural progenitor cell proliferation in some CNS regions, while inhibiting it in others (de la Mano, Gato et al. 2007). This could contribute to the region-specific overgrowth and undergrowth observed in the ASD brain. Excitatory synapse formation is partially mediated by the IL-1 receptor and receptor-associated proteins (Yoshida, Yasumura et al. 2011).
Altering these proteins can tip the balance between excitatory and inhibitory signaling, which might underlie neurological features of autism (Rubenstein and Merzenich 2003). Increased IL-1ra in autism suggests an attempt to counterbalance IL-1Β and may or may not be beneficial. Following brain injury, IL-1ra upregulation serves a neuroprotective role by dampening excessive inflammation (Loddick and Rothwell 1996). However, if administered during critical windows of neurodevelopment, IL-1ra can negatively impact neurogenesis, brain morphology, memory consolidation, and behavior (Spulber, Oprica et al. 2008; Spulber, Bartfai et al. 2010; Spulber, Bartfai et al. 2011). This shows that some level of IL-1B signaling is essential during development. In adulthood, IL-1Β is implicated in CNS disorders like Alzheimer's disease and the advancement of amyloid-containing plaques (Griffin, Sheng et al. 1995). While excessive IL-1B contributes to pathology in some cases, it may have a protective role in others. For example, IL-1Β limits neuronal damage following excitotoxic exposures (Strijbos and Rothwell 1995), and mice lacking IL-1Β fail to undergo remyelination following experimental autoimmune encephalitis (EAE) induction (Mason, Suzuki et al. 2001). IL-1Β is involved in higher order brain processes and is induced in the hippocampus during learning processes, and is critical for maintenance of long-term potentiation (LTP) (Ross, Allan et al. 2003). Both over expression (Barrientos, Frank et al. 2009) and under expression of IL-1 beta (Goshen, Kreisel et al. 2007; Labrousse, Costes et al. 2009)are associated with impairments in memory and learning.
In summary, IL-1Β participates in neurological processes, and appears to have a role in both CNS pathology and healing. Normal, homeostatic levels of IL-1Β and its antagonist IL-1ra are necessary for proper brain development and function. This "Goldilocks" state is typical of many cytokines, where too much or too little is not desirable. Alterations in IL-1Β systems due to genetic mechanisms or environmental exposures may contribute to autism.
IL-6 is an inflammatory cytokine that shares functional properties with IL-1Β. Like IL-1Β, IL-6 is produced early in immune reactions, although it appears later and persists longer (Jiang, Tian et al. 1997). IL-6 is best known for stimulating the acute phase response in the liver, generating fever, and activating lymphocytes. Despite the functional similarities with IL-1Β, IL-6 differs drastically in terms of structure and signaling properties. It is a member of the neuropoietic cytokine family, which includes leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), and IL-11. These cytokines signal through a gp130 receptor complex (Ward, Howlett et al. 1994), and activate JAK-STAT (specifically STAT 3) and MAPK signaling pathways (Heinrich, Behrmann et al. 2003). In addition to their inflammatory properties, neuropoietic cytokines have a number of well-described roles in the nervous system, and are intricately involved in neurodevelopment and function (Bauer, Kerr et al. 2007; McAfoose and Baune 2009). IL-6 and its receptors are expressed at low levels in the healthy brain (Gadient and Otten 1994; Gadient and Otten 1994) and at higher levels in a variety of disease states (Huell, Strauss et al. 1995; Hang, Shi et al. 2004). Peripheral IL-6 can cross the blood brain barrier and influence a variety of processes in the adult brain (Banks, Kastin et al. 1994).
Prenatal cytokine imbalances may contribute to neurodevelopmental disorders like autism and schizophrenia through "fetal programming". Fetal programming is the concept that maternal factors like inflammation and chronic stress can alter the gestational environment, skew development, and lead to long term physiological and behavioral consequences (Patterson 2009; Bilbo 2010). IL-6 readily crosses the placenta and enters fetal tissues, which is unique among cytokines, and can induce changes in placental physiology and gene expression (Zaretsky, Alexander et al. 2004; Aaltonen, Heikkinen et al. 2005; Dahlgren, Samuelsson et al. 2006; Hsiao and Patterson 2011). Animal models show that IL-6 is necessary and sufficient to alter neurodevelopmental outcomes, leading to changes in behavior, cognition, neuropathology, GABA dysregulation, and skewed immune function among offspring (Samuelsson, Jennische et al. 2006; Smith, Li et al. 2007). Similar effects are seen with prenatal exposure to infection or the infectious mimic poly I:C (Smith, Li et al. 2007; Malkova, Yu et al. 2012). IL-6 can impact a variety of processes in the developing brain. IL-6 and its family members regulate self-renewal among neuronal precursors (Escary, Perreau et al. 1993; Yoshimatsu, Kawaguchi et al. 2006), direct neuronal migration (Wei, Zou et al. 2011), promote cell survival (Kushima, Hama et al. 1992), and regulate neurite outgrowth (Ihara, Nakajima et al. 1997). IL-6 exposure during critical windows can also alter synaptic networks. Chronic IL-6 overexpression reduces expression of glutamate receptors and L-type calcium channels in culture and in vivo (Vereyken, Bajova et al. 2007), and increases the ratio of excitatory to inhibitory synapses in cultures of cerebellar granular cell cultures (Wei, Zou et al. 2011). This is of particular interest in autism, given that skewed excitatory and inhibitory ratios may be an underlying factor in its pathogenesis.
Despite intriguing evidence from animal models, two recent human studies question whether gestational IL-6 alone contributes to autism. A retrospective examination of IL-6 in archived mid-pregnancy maternal serum and amniotic fluid showed that increased levels associated with developmental disorders, but not autism (Abdallah, Larsen et al. 2011; Goines, Croen et al. 2011). This suggests that gestational IL-6 might be a marker for neurodevelopmental diseases, but is insufficient on its own to cause ASD. IL-6 can affect many sequential phases of neurodevelopment, so the timing of the exposure will largely dictate the neurological outcome. Therefore, excessive IL-6 during one phase of neurodevelopment could have one set of consequences, while similar expression during another phase has an entirely different effect. A longitudinal examination of IL-6 throughout gestation is therefore needed to obtain a more complete picture of its relevance in neurodevelopmental disorders.
IL-6 can also impact processes in the adult brain, and physiological levels are critical for homeostasis, cognition, learning, and memory. Physiological levels of IL-6 are critical for normal CNS function, and both over and under expression leads to neurological problems. Mice overexpressing IL-6 in the CNS have overt symptoms including tremor, ataxia, and seizure (Campbell, Abraham et al. 1993), and more subtle alterations in cognition and avoidance behaviors (Heyser, Masliah et al. 1997). IL-6 is transcribed in the hippocampus during LTP (Balschun, Wetzel et al. 2004). Overexpression of IL-6 reduces LTP (Bellinger, Madamba et al. 1995; Li, Katafuchi et al. 1997), whilst under expression increases it and improves learning and memory (Balschun, Wetzel et al. 2004; Braida, Sacerdote et al. 2004). With regard to social behaviors, mice overexpressing IL-6 are more social than mice that lack the cytokine, while mice lacking IL-6 demonstrate higher aggression and emotionality (Alleva, Cirulli et al. 1998; Armario, Hernandez et al. 1998).
Many independent studies show IL-6 dysregulation in individuals with autism. Children and adults with the disorder have higher circulating IL-6 levels compared to typical controls (Emanuele, Orsi et al. 2010; Ashwood, Krakowiak et al. 2011). Further, cellular IL-6 production is increased with and without stimulation (Jyonouchi, Sun et al. 2001; Enstrom, Onore et al. 2010; Malik, Sheikh et al. 2011). Increased IL-6 is also found in postmortem brain specimens from ASD subjects. Specifically, immunohistochemical analysis of cerebellar sections showed significantly more IL-6 staining in autism postmortem brain specimens (Wei, Zou et al. 2011). Two additional analyses of homogenates of the frontal cortex and anterior cingulate gyrus also showed higher IL-6 levels (Vargas, Nascimbene et al. 2005; Li, Chauhan et al. 2009). Given the ability of IL-6 to impact processes in the adult brain, it is conceivable that increased IL-6 in autism could contribute to ongoing aspects of the disorder. Alternatively, it might be an epiphenomenon, and represent a biomarker of infectious or toxic environmental exposures and altered biological homeostasis.
In summary, there is extensive evidence that IL-6 can alter neurodevelopment and function. While it is unclear whether gestational IL-6 in humans is related to autism, a dysregulation of IL-6 is observed later in life in individuals with autism. The significance of these findings is unclear, and may be the result of other genetic and environmental factors in autism. These possibilities warrant further investigation.
IL-4 is a class I cytokine that activates Jak/Stat (STAT 6), MAPK, and PI3 kinase signaling cascades (Nelms, Keegan et al. 1999). Immunologically, IL-4 has a variety of interesting roles, and can 1) induce "alternatively activated" macrophages that promote tissue repair over inflammation 2) activate basophils and mast cells, 3) promote B-cell isotype switching towards IgG1 and IgE, 4) participate in immune responses against helminthes by inducing epithelial cell turnover in the gut, and 5) participate in allergy and asthma-related immune responses (Kuperman and Schleimer 2008; Byers and Holtzman 2011; Oliphant, Barlow et al. 2011).
The receptors for IL-4 are expressed in the brain under normal conditions throughout life (Nolan, Maher et al. 2005). During development, IL-4 promotes oligodendrogenesis among neuronal progenitor cells, (Butovsky, Ziv et al. 2006), and improves survival in embryonic hippocampal cultures (Araujo and Cotman 1993). IL-4 influences retinal circuitry by regulating progenitor cell proliferation and differentiation (da Silva, Campello-Costa et al. 2008). During later phases of neurodevelopment, IL-4 can alter synapse formation; increasing the proportion of GABAergic synapses in cell culture models (Sholl-Franco, Marques et al. 2002).
Two recent studies have linked developmental IL-4 exposures to autism, though its role in pathogenicity versus protection is unclear. Mothers giving birth to a child with autism show higher levels of IL-4 in mid-pregnancy serum samples (Goines, Croen et al. 2011) and amniotic fluid (Abdallah, Larsen et al. 2011) compared to controls. IL-4 is not thought to cross the placenta, and maternal serum and amniotic fluid IL-4 may or may not relate to IL-4 in fetal tissues. Other cytokines were also upregulated in these archived samples, including IFN-γ, TNF-α, and the anti-inflammatory cytokine IL-10. This raises the question of whether IL-4 acts alone, or in concert with other cytokines. Increased IL-4 may represent a regulatory reflex to inflammation along with IL-10. IL-4's role in pregnancy and fetal health is unclear. Increased levels during pregnancy have been associated with poor outcomes such as preterm labor (Dudley, Hunter et al. 1996) but also healthy outcomes such as protection from preeclampsia (Kronborg, Gjedsted et al. 2011; Rajakumar, Chu et al. 2011). More subtle neurodevelopmental outcomes have not thoroughly been explored with respect to gestational IL-4.
In the adult brain, IL-4 largely serves a neuroprotective role, and is associated with higher order cognitive processes. It is upregulated during CNS inflammation, inducing alternative activation of glial cells and protecting from apoptosis (Garg, Kipnis et al. 2009; Sholl-Franco, da Silva et al. 2009; You, Luo et al. 2011). In a mouse model for Alzheimer's disease, IL-4 can attenuate disease progression (Kiyota, Okuyama et al. 2010). Following LPS exposure, IL-4 reduces inflammation and improves memory and LTP in the aged hippocampus (Nolan, Maher et al. 2005). An elegant study by Derecki et al showed that IL-4-producing T cells accumulate in the meningeal spaces during cognitive tasks. Depletion of IL-4 led to an inflammatory phenotype among meningeal myeloid cells, and a dramatic decline in cognitive capacity. Remarkably, cognitive deficits in IL-4 deficient mice could be reversed by reintroducing the cytokine in adulthood (Derecki, Cardani et al. 2010).
Among individuals diagnosed with autism, plasma and CNS IL-4 levels appear to be normal (Vargas, Nascimbene et al. 2005; Li, Chauhan et al. 2009; Ashwood, Krakowiak et al. 2011). However, IL-4 producing T cells are proportionately higher in children with autism compared to controls (Gupta, Aggarwal et al. 1998). Given the evidence that meningeal IL-4 producing T cells are critical for normal cognitive function in adulthood, it is possible that dysregulation in this cell population could contribute to altered behavior throughout life (Derecki, Cardani et al. 2010).
Collectively, IL-4 serves a variety of neurological roles, and is increased in autism. Its role during gestation is unclear due to a dearth of in-vivo studies of pregnancy and neurobehavioral outcomes following developmental IL-4 exposures. The significance of increased IL-4 producing T cells in subjects with autism is also unclear. Extensive evidence suggests that IL-4 is neurologically beneficial, so it may be that increased IL-4 in autism represents an immunological attempt to regulate other detrimental processes, and does not contribute to the disease itself. Future studies should explore this possibility.
Interferon gamma (IFN-γ) is the sole type II interferon. It shares some functional similarities with type I interferons like IFN-α and IFN-Β but has unique structural features, receptors, and signaling pathways. IFN-γ is produced primarily by T cells and Natural Killer (NK) cells during cell-mediated immune responses, and functions largely to activate macrophages and combat viral infections (Boehm, Klamp et al. 1997; Schroder, Hertzog et al. 2004). It signals mainly through the JAK/STAT (STAT1), and MAPK cascades (Hu, Roy et al. 2001; Platanias 2005). IFN-γ and IL-4 counterbalance one another's activity via TH1/TH2 interactions, so dysregulation in one cytokine often impacts the other. It is therefore not surprising that both cytokines are implicated in ASD.
Developmental exposure to IFN-γ has been linked to autism. Mothers of children with autism have higher serum IFN-γ during the second trimester compared to controls (Goines, Croen et al. 2011). Like IL-4, IFN-γ does not cross the placenta, and the relationship between maternal serum levels and fetal exposure to the cytokine is unclear. If the cytokine is present in fetal tissues, it could interfere with normal neural development and synapse formation. IFN-γ promotes neuronal differentiation among neural progenitor cells (Barish, Mansdorf et al. 1991; Jonakait, Wei et al. 1994; Wong, Goldshmit et al. 2004; Butovsky, Ziv et al. 2006; Zahir, Chen et al. 2009; Leipzig, Xu et al. 2010; Li, Walker et al. 2010), however, these cells appear to be abnormal and exhibit compromised function and strange patterns of neuronal marker expression (Walter, Honsek et al. 2011). IFN-γ also impacts dendritic morphology and synapse formation, leading to long-term changes in cellular connectivity and communication. Depending on cell culture conditions, IFN-γ either promotes or inhibits dendrite outgrowth through STAT 1 and MAPK signaling pathways (Barish, Mansdorf et al. 1991; Kim, Beck et al. 2002; Wong, Goldshmit et al. 2004; Song, Wang et al. 2005; Andres, Shi et al. 2008). In culture, excessive IFN-γ alters patterns of excitatory signaling and receptor expression (Vikman, Owe-Larsson et al. 2001), and animals lacking the cytokine have fewer pre-synaptic terminals (Victorio, Havton et al. 2010). Interestingly, mice overexpressing IFN-γ show increased MHC I in the brain (Corbin, Kelly et al. 1996). MHC I is critical for T cell and NK cell recognition of self and foreign entities, and was historically thought to be absent in the CNS. However, recent studies have demonstrated that it is expressed in the CNS, and has an essential role in synapse formation and plasticity (Shatz 2009). IFN-γ may therefore induce abnormalities in synaptic organization by altering MHC I expression. Collectively, these studies show that direct exposure to IFN-γ can cause abnormal neurodevelopment, which may explain features of autism.
If excess IFN-γ is not present in fetal tissues, excessive maternal levels could have an indirect impact on fetal development. Interestingly, IFN-γ has a variety of critical roles in pregnancy, and directs aspects of placental development, health, and maintenance (Murphy, Tayade et al. 2009). Increased gestational IFN-γ is associated with adverse pregnancy outcomes including recurrent miscarriage (Jenkins, Roberts et al. 2000). Therefore, IFN-γ might be an indicator of compromised health in pregnancy, which could lead to neurodevelopmental abnormalities.
Peripheral IFN-γ is up-regulated in a number of neurological disorders including multiple sclerosis (Martins, Rose et al. 2011) and Down's syndrome (Torre, Broggini et al. 1995). Individuals with autism also have increased plasma levels of IFN-γ (Singh 1996), which correlates with other peripheral inflammatory mediators such as nitric oxide (Sweeten, Posey et al. 2004). Peripheral immune cells from ASD subjects produce higher basal levels of IFN-γ but fail to respond further following immunological stimulation (Gupta, Aggarwal et al. 1998; Croonenberghs, Bosmans et al. 2002; Enstrom, Lit et al. 2009). In addition to peripheral IFN-γ dysregulation, postmortem brain specimens showed increased levels of IFN-γ (Li, Chauhan et al. 2009), suggesting IFN-γ may directly impact CNS processes in autism. In the developed nervous system, IFN-γ is historically associated with neurodegeneration, although some evidence suggests it may have a beneficial role. IFN-γ can cross the blood brain barrier at low levels (Pan, Banks et al. 1997), but is barely detectable in the healthy nervous system (Traugott and Lebon 1988; De Simone, Levi et al. 1998). In the CNS, IFN-γ is up-regulated following infectious exposures (De Simone, Levi et al. 1998), and in diseases including cerebral palsy (Folkerth, Keefe et al. 2004), multiple sclerosis (Traugott and Lebon 1988), HIV dementia (Nolting, Lindecke et al. 2009), and Parkinson's (Barcia, Ros et al. 2011; Mangano, Litteljohn et al. 2011). High levels are harmful in cell culture, and cause enhanced glutamate induced neurotoxicity (Mizuno, Zhang et al. 2008). However, in cell culture and in vivo, low IFN-γ levels reduce oxidative-stress induced apoptosis through activation of astrocytes (Garg, Kipnis et al. 2009; Victorio, Havton et al. 2010) and may be neuroprotective in some cases. In a mouse model of Alzheimer's disease, overexpressing IFN-γ actually attenuated plaque formation (Chakrabarty, Ceballos-Diaz et al. 2010), whilst protective roles for IFN-γ have been suggested during some phases of demyelaniting autoimmune disorders (Kumar and Sercarz 1998). It is therefore difficult to determine whether IFN-γ has a pathogenic role in ASD, or if it represents a potentially beneficial immune response to damage associated with genetic and/or environmental influences.
TGF-Β is a highly pleiotropic cytokine that maintains immune homeostasis, directs lymphocyte differentiation, and orchestrates aspects of embryonic development. TGF-Β is largely immunosuppressive; limiting excessive T cell activity and inflammation (Mantel and Schmidt-Weber 2011). It exists in three isoforms, each with distinct and overlapping roles. TGF-Β1 is best characterized, and is the founding member of the TGF-Β superfamily of proteins, which includes growth differentiation factors, bone morphogenic proteins (BMPs), activins, and inhibins (Kingsley 1994). TGF-Β superfamily signaling occurs largely via SMAD pathways, though MAPK cascades are also triggered (Yu, Hebert et al. 2002; Shi and Massague 2003).
TGF-Β superfamily proteins are critical for proper neurodevelopment. For example, BMPs have an important role in early neural induction and differentiation (Reissmann, Ernsberger et al. 1996; Bachiller, Klingensmith et al. 2000; Tropepe, Hitoshi et al. 2001). TGF-Β1 is involved in neuronal migration, survival, and synapse formation. Mice lacking the cytokine demonstrate improper CNS development, including a disorganized extracellular matrix, widespread neuronal degeneration, microgliosis, reduced expression of synaptophysin, and deficits in both glutamatergic and GABAergic synapses (Brionne, Tesseur et al. 2003; Heupel, Sargsyan et al. 2008; Vashlishan, Madison et al. 2008). Overexpressing TGF-Β in vivo also disrupts the extracellular matrix, and leads to seizures, motor incoordination, hydrocephalus, and behavioral abnormalities (Wyss-Coray, Feng et al. 1995; Depino, Lucchina et al. 2011). Changes in CNS expression levels of IL-6, Neuroligin 3 and reelin are also observed with TGF- Β overexpression (Depino, Lucchina et al. 2011), which is intriguing because many of these proteins are altered in autism (Persico, D'Agruma et al. 2001; Laumonnier, Bonnet-Brilhault et al. 2004; Fatemi, Snow et al. 2005). Interestingly, CNS overexpression of TGF-Β during development vs. adulthood leads to opposite behavioral consequences (Depino, Lucchina et al. 2011). Early in life, overexpression led to decreased social behavior and heightened anxiety/depression behaviors, while overexpression later in life had the exact opposite effect. This highlights the importance of timing when considering the neurological consequences of cytokine imbalances.
There is no evidence for TGF-Β dysregulation during gestational development in autism. This doesn't negate the possibility that it is involved, as these endpoints are extremely difficult to measure in vivo. However, there is evidence for TGF-Β dysregulation in individuals diagnosed with the disorder themselves. Plasma TGF-Β is decreased in children and adults with ASD (Okada, Hashimoto et al. 2007; Ashwood, Enstrom et al. 2008), and lower levels of the cytokine correlate with more severe autism behaviors (Ashwood, Enstrom et al. 2008). The connection between low TGF-Β and behavioral phenotype is unclear, although this finding lends promise to the goal of developing a simple ASD testing regime based on biological markers in addition to behavioral symptoms. In contrast to peripheral TGF-Β in ASD, postmortem brain specimens show increased levels compared to controls (Vargas, Nascimbene et al. 2005). The reason for this periphery/brain disparity is unclear. Although, TGF-Β does not cross the blood brain barrier (Kastin, Akerstrom et al. 2003), the cytokine and its receptors are expressed normally throughout the nervous system (Gomes, Sousa Vde et al. 2005) and it is conceivable that peripheral and brain levels are independent. In the adult brain, TGF-Β is generally thought to be neuroprotective. CNS levels spike following injury and infection, and increase with age, HIV dementia, and Alzheimer's disease, which leads to protection against disease-related neural degeneration and apoptosis (Henrich-Noack, Prehn et al. 1994; Krupinski, Kumar et al. 1996; Ruocco, Nicole et al. 1999; Buckwalter and Wyss-Coray 2004; Doyle, Cekanaviciute et al. 2010). However, TGF-Β can also contribute to pathogenicity. For example, high levels of the cytokine cause glial scarring and fibrosis (Moon and Fawcett 2001), and increase disease susceptibility and severity in a murine model of multiple sclerosis (Wyss-Coray, Borrow et al. 1997).
Overall, the role of TGF-Β in the nervous system is complex, and varies based on the timing and context of the interaction. Increased TGF-Β in the brain of ASD subjects might represent a protective reflex to a disease state, or perhaps contribute to the pathology of the disease itself. In the periphery, ASD subjects have decreased TGF-Β as well as increased inflammatory markers (Jyonouchi, Sun et al. 2001; Croonenberghs, Bosmans et al. 2002; Emanuele, Orsi et al. 2010; Ashwood, Krakowiak et al. 2011; Ashwood, Krakowiak et al. 2011). This suggests a global immune dysregulation in ASD, with an improper balance between regulation and activation, which could have wide reaching consequences for many systems in the body.
There is now general consensus that autism has an environmental component (Pessah 2008; Hallmayer, Cleveland et al. 2011). For our purposes, the "environment" is a broad term used to define non-genetic, toxic, infectious, and/or immune factors that may contribute to the disorder. The hypothesis follows that an ill-timed exposure could cause autism in genetically susceptible individuals (Figure 1). For example, some genes associated with autism may cause inappropriate immune responses (Heuer 2011; Onore, Careaga et al. 2011), while others reduce the capacity to deal appropriately with toxins (D'Amelio, Ricci et al. 2005). These individuals are more susceptible to environmental influences, which could lead to ASD.
Environmental toxicants can cause both neural and immune dysfunction. This is likely mediated through disrupted cell signaling and homeostasis. Many toxicants alter calcium homeostasis, which can have a variety of consequences for immune development and function (Limke, Otero-Montanez et al. 2003; Toscano and Guilarte 2005; Savignac, Mellstrom et al. 2007; Vig and Kinet 2009; Pessah, Cherednichenko et al. 2010; Bhatti, Bhatti et al. 2011). Toxicants can also disrupt endocrine function, which can have a variety of immunological consequences (Rivest 2010; De Vito, Incerpi et al. 2011; Schug, Janesick et al. 2011). The following section considers environmental factors that may be related to autism, and focuses on their role in the immune system.
Heavy metals like lead and mercury are widespread environmental toxins. Developmental exposure to these compounds is associated with lower IQ, endocrine disruptions, and behavioral disturbances (Bellinger and Dietrich 1994; Steuerwald, Weihe et al. 2000; Cordier, Garel et al. 2002; Selevan, Rice et al. 2003; Winneke 2011). Heavy metals also have immunotoxic properties, leading in some instances to autoantibody production (Waterman, el-Fawal et al. 1994; Bagenstose, Salgame et al. 1999; Rowley and Monestier 2005) and skewed cytokine profiles (discussed below). Both of these immunological phenomena are observed in ASD (Enstrom, Van de Water et al. 2009; Onore, Careaga et al. 2011). These features have made them possible, though controversial, candidates in autism.
Lead has a variety of toxic mechanisms, and shares structural features with calcium which allows it to compete for binding sites (Toscano and Guilarte 2005). In addition to its neurotoxic activity, lead is highly immunotoxic (Toscano and Guilarte 2005; Mishra 2009). At high levels, it is immunosuppressive, leading to increased production of regulatory cytokines and enhanced susceptibility to infection (Valentino, Rapisarda et al. 2007). At lower levels, lead appears to be immunostimulatory (Flohe, Bruggemann et al. 2002). One study showed that lead can tip the balance between inflammation and regulation by increasing expression of IFN-γ and reducing TGF-Β (Goebel, Flohe et al. 2000): a cytokine profile which has been observed in autism (Singh 1996; Gupta, Aggarwal et al. 1998; Croonenberghs, Bosmans et al. 2002; Ashwood, Enstrom et al. 2008; Enstrom, Lit et al. 2009; Li, Chauhan et al. 2009). Lead and pro-inflammatory cytokines can function in concert to alter the nervous system. When coadministered to glial cells, lead and cytokines such as IL-1Β changed matrix metalloproteinase expression in a manner that was not observed when either was administered alone (Lahat, Shapiro et al. 2002) and suggests that immune and environmental factors could act synergistically on tissue remodeling.
There are conflicting reports regarding lead in autism. Higher serum lead levels have been documented in a few studies (Cohen, Johnson et al. 1976; Shannon and Graef 1996; Filipek, Accardo et al. 1999), although more recent studies show no difference between autism and control populations (Tian, Green et al. 2011; Albizzati, More et al. 2012). Polymorphisms in ALAD, a gene associated with heavy metal toxicity, have been described in ASD (Rose, Melnyk et al. 2008) leading, some authors to speculate that children with ASD have higher lead levels due to a decreased capacity to eliminate lead from the body (Kern, Grannemann et al. 2007). Others suggest increased frequency of pica (hand-to-mouth behaviors) in ASD may also alter exposures to lead (Cohen, Johnson et al. 1976; Shannon and Graef 1996). Despite lead's well-established neurotoxic and immunotoxic mechanisms, it is currently debatable whether it contributes to autism.
Mercury exerts its toxicity broadly throughout the body. It binds a wide range of molecular groups including thiols, hydroxyls, and carboxyls (Bridges and Zalups 2010), and can dramatically increase intracellular calcium levels (Sakamoto, Ikegami et al. 1996; Limke, Otero-Montanez et al. 2003). In the immune system, mercury's effects depend heavily on genetic background. For example, certain strains of mice reliably develop anti-nuclear antibodies after mercury exposure, while others do not (Bagenstose, Salgame et al. 1999; Rowley and Monestier 2005). Mercury also impacts cytokine profiles; and some strains of mice strongly up-regulate the TH2 cytokine IL-4 in response to mercury, while other strains up-regulate TH1 cytokines like IFN-γ (Wu, Turner et al. 2001; Hemdan, Lehmann et al. 2007). In two human mast cell lines, mercury exposure was shown to increase IL-6 production (Kempuraj, Asadi et al. 2010). Further, mercury interferes with cytokine-related signaling cascades including NF-κB and p38 MAPK (Dieguez-Acuna, Ellis et al. 2001; Kim, Johnson et al. 2002). Altered cytokine repertoires in response to mercury exposure may contribute to the development of autoimmunity. Interestingly, IL-4 deficient mice produce autoantibodies in response to mercury exposure (Bagenstose, Salgame et al. 1998; Kono, Balomenos et al. 1998), while IFN-γ deficient mice do not (Kono, Balomenos et al. 1998), suggesting that autoantibody production is IFN-γ dependent.
The evidence implicating mercury in autism is somewhat contradictory. Several recent studies have shown no link between mercury body burdens and autism (Ip, Wong et al. 2004; Hertz-Picciotto, Green et al. 2010; Albizzati, More et al. 2012; Wright, Pearce et al. 2012). However, a reanalysis of data generated by Ip et al showed there was in fact an association between mercury levels and autism that had been overlooked due to a statistical error (Desoto and Hitlan 2007). Ethyl mercury is a component in the vaccine preservative thimerosal, which has received attention in recent years. It has neurotoxic capacities, and can alter calcium signaling and cytokine production (specifically IL-6) (Goth, Chu et al. 2006). While in vitro studies suggest toxic potential for thimerosal, a large number of independent epidemiological studies show no link to autism (DeStefano 2007; Miller and Reynolds 2009; Price, Thompson et al. 2010).
Two recent studies examined the correlation between gene transcription profiles (35,000+ genes) and heavy metal body burdens in children with autism and controls (Stamova, Green et al. 2011; Tian, Green et al. 2011). Mercury loads correlated with the expression of several immunologically relevant genes across all study participants. Further, there were some unique correlations in the autism group for genes involved in antigen presentation and recognition of self. This suggests that individuals with ASD may have a unique immunological susceptibility to heavy metals, but the significance of these findings is not clear. Although genomic expression profiles may suggest correlative changes in autism, analysis of common polymorphisms associated with mercury transport and excretion, namely MT1a, DMT1, LAT1, and MTF1, were unable to detect any differences in autism (Owens, Summar et al. 2011). However, in contrast, a different study showed that polymorphisms in MTF1 and the heavy metal transport gene (SLC11A3) are associated with the ASD(Serajee, Nabi et al. 2004). Additional genetic analyses are needed to rectify these disparate findings.
Pesticides are fairly non-persistent toxic compounds that are deliberately spread throughout the environment in mass quantities. While minimizing off-target toxicity is a primary goal in pesticide development, several products have been banned once their toxic potential in humans was recognized. Developmental exposure to several types of pesticides, including organophosphates (OPs), organochlorines (OCs), and pyrethroids, is associated with neurological dysfunction and an increased risk for ASD (Garry, Harkins et al. 2002; Kamel and Hoppin 2004; Eskenazi, Marks et al. 2007; Roberts, English et al. 2007; Eskenazi, Huen et al. 2010; Bouchard, Chevrier et al. 2011). Genetic analyses also suggest that individuals with ASD may be less capable of excreting pesticides, due to expression of a less-active variant of the OP-metabolizing enzyme paroxonase (D'Amelio, Ricci et al. 2005; Pasca, Nemes et al. 2006). In addition to their neurotoxicity, many pesticides impact the immune system and cytokine production, which may be relevant for ASD (Banerjee, Koner et al. 1996; Li 2007).
Organochlorine (OC) pesticides are structurally and functionally variable toxic compounds that include members like hexachlorobenzene, dicofol, and DDT; many of which have been banned (Crinnion 2009). OCs interferes with calcium signaling, voltage sensitive sodium channels, and GABA receptors, leading to neuro- and immunotoxicity (Casida 2009; Crinnion 2009; Heusinkveld and Westerink 2012). Immunologically, these compounds impact both humoral and cell-mediated processes, and reduce the host response to infectious challenges (Banerjee, Koner et al. 1996; Reed, Dzon et al. 2004; Nagayama, Tsuji et al. 2007). A handful of studies have explored the impact of OCs on cytokine profiles. DDT reduced IL-2 production in cell culture by interfering with the transcription factor NF-κB (Ndebele, Tchounwou et al. 2004). Given the central role of NF-κB in cytokine production and function, this is likely to have a wide-ranging immunological impact. In contrast, cases of DDT or lindane poisoning in humans is associated with increased serum levels of IL-2, IL-4, and TNF-alpha, as well as decreased levels of IFN-γ (Daniel, Huber et al. 2002; Seth, Ahmad et al. 2005). It is not clear why IL-2 production in response to OCs differs in cell culture versus in vivo. However, the findings of increased IL-4 and decreased IFN-γ in humans suggest a TH2 immune bias following OC exposure, which could have downstream consequences for allergic and asthmatic disorders. Similar immune profiles have been reported in some studies of autism (Gupta, Aggarwal et al. 1998).
Organophosphates (OP) are esters of phosphoric acid that were introduced as replacements for various banned OC pesticides. They act primarily through acetylcholinesterase (AChE) inhibition, leading to altered cholinergic signaling, parasympathetic and sympathetic perturbations, seizures, and/or respiratory arrest. OPs can also be toxic in the absence of AChE inhibition, and may induce higher order neural and cognitive dysfunction (Duysen, Li et al. 2001; Costa 2006; Pancetti, Olmos et al. 2007). Some OP developmental effects are more severe in males than females (Levin, Addy et al. 2001; Levin, Timofeeva et al. 2010), which is intriguing given that autism also has a heavy male bias (Baron-Cohen, Knickmeyer et al. 2005).
OPs induce a variety of immunological phenomena relevant to autism (Galloway and Handy 2003; Li 2007). In general, OPs appear to induce a prolonged inflammatory state that may evolve into an adaptive response characterized by up-regulation of both TH1 and TH2 cytokines. Acute OP intoxication is related to system-wide production of inflammatory mediators (Hamaguchi, Namera et al. 2006; Roeyen, Chapelle et al. 2008; Anand, Singh et al. 2009). Within the CNS, acute and chronic exposure to OPs results in increased inflammatory cytokines including IL-1Β and IL-6 in multiple brain regions (Svensson, Waara et al. 2001; Henderson, Barr et al. 2002; Williams, Berti et al. 2003; Dhote, Peinnequin et al. 2007; Dillman, Phillips et al. 2009; Johnson and Kan 2010); similar to findings in the autism brain (Vargas, Nascimbene et al. 2005; Li, Chauhan et al. 2009). OP induced inflammation can be long term, re-emerging and persisting long after the initial exposure (Chapman, Kadar et al. 2006). In primary cultures of human peripheral blood cells or astrocytes, the OP pesticide chlorpyrifos up-regulates IL-6 and IFN-γ production and the expression of related genes (Mense, Sengupta et al. 2006). Children born to mothers working in agriculture had higher production of TH2 cells at 12 and 24 months of age. (Duramad, Harley et al. 2006; Duramad, Tager et al. 2006). An immunosuppressive response can also be induced following exposure to various OPs, perhaps as a reflex to the toxin's initial inflammatory properties (Williams, Berti et al. 2003; Damodaran, Greenfield et al. 2006; Dhote, Peinnequin et al. 2007). Collectively, these data suggest that OPs impact cytokine profiles in the short and long term, increasing inflammatory cytokines, TH1 and TH2 profiles and compensatory regulatory activity.
Pyrethroids are a group of insecticides and repellants derived from natural compounds in the Chrysanthemum genus of plants. They mediate their toxicity by disrupting calcium signaling, interfering with voltage sensitive sodium channels, and inducing oxidative stress (Shafer, Meyer et al. 2005; Soderlund 2012). Exposure to these compounds is associated with a wide range of neurodevelopmental problems in mammalian models (Shafer, Meyer et al. 2005; Wolansky and Harrill 2008). Immunological abnormalities are also linked to pyrethroids. In human peripheral blood mononuclear cells, exposure to several different pyrethroid compounds suppressed both IFN-γ and IL-4 expression in a time and concentration dependent manner (Diel, Horr et al. 2003). In a monocytic cell line, various synthetic pyrethroids and their metabolites reduced expression of immunoregulatory IL-10, and increased production of more inflammatory cytokines IL-12 and TNF-α (Zhang, Zhao et al. 2010). In a Xenopus laevis model, application of environmentally relevant concentrations of various pyrethroids increased IL-1Β expression (Martini, Fernandez et al. 2010). In primary human fetal astrocytes, the pyrethroid pesticide cyfluthrin was found to have an activating effect, and increased the expression of genes involved in IFN-γ and IL-6 production and signaling (Mense, Sengupta et al. 2006)
Halogenated aromatic hydrocarbons are toxic compounds that are highly resistant to degradation. Two examples are polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs). PCBs and PBDEs consist of two aromatic rings with various chlorine (PCBs) or bromine (PBDEs) substitutions. There are over two hundred different congeners each of PCBs and PBDEs, which differ based on the number and orientation of halogen substitutions. Toxicity in this class of molecules is congener-specific, and involves varying degrees of 1) interaction with the Aryl hydrocarbon receptor (Ahr) (Mitchell and Elferink 2009; Gu, Goodarzi et al. 2012), 2) disruption of endocrine systems (Morse, Groen et al. 1993; Van Birgelen, Smit et al. 1995; Stoker, Cooper et al. 2005; Kuriyama, Wanner et al. 2007; Lema, Dickey et al. 2008), and/or 3) interference with calcium homeostasis through interactions with the ryanodine receptor (Coburn, Curras-Collazo et al. 2008; Pessah, Lehmler et al. 2009; Pessah, Cherednichenko et al. 2010; Kim, Bose et al. 2011; Langeveld, Meijer et al. 2012). These toxic mechanisms have both neurological and immunological significance.
Children with ASD may be uniquely susceptible to halogenated aromatic hydrocarbons. Postmortem analysis showed altered ryanodine receptor expression in the brain of autism subjects compared to controls, which could alter their sensitivity to Ryr-reactive compounds (Voineagu, Wang et al. 2011). Further, in a mouse model of Rett syndrome, a genetic disorder that shares features with autism, developmental exposure to PBDE-47 caused epigenetic, cognitive, and social differences that were not observed in wild type mice (Amir, Van den Veyver et al. 1999; Woods, Vallero et al. 2012). Finally, children with ASD have unique immune responses to PBDEs, which is discussed in detail below (Ashwood, Schauer et al. 2009).
Polychlorinated biphenyls (PCBs) are ubiquitous in the environment and in animal and human tissues. They were widely used as additives to industrial oils and lubricants until the late 1970's when their adverse health effects became apparent. PCB exposure is linked to adverse pregnancy outcomes and neurobehavioral deficits (Kuratsune, Yoshimura et al. 1971; Rogan, Gladen et al. 1988; Chen and Hsu 1994; Eriksson and Fredriksson 1998; Howard, Fitzpatrick et al. 2003; Kenet, Froemke et al. 2007; Lein, Yang et al. 2007; Tsukimori, Tokunaga et al. 2008; Kim, Inan et al. 2009; Boix, Cauli et al. 2010; Kim and Pessah 2011), as well as immune dysfunction. In studies involving marine animals, murine models, and humans, PCBs lead to generalized immune suppression, characterized by diminished cellular immunity and atrophied lymphoid organs (Davis and Safe 1990; Narayanan, Carter et al. 1998; Fournier, Degas et al. 2000; Shin, Bae et al. 2000; Tan, Li et al. 2003; Beineke, Siebert et al. 2005; Leijs, Koppe et al. 2009). Cytokine profiles are also impacted by PCBs. In a murine model, perinatal exposure to a PCB mixture induced inflammatory cytokine expression (primarily IL-6) in the brain of adult offspring (Hayley, Mangano et al. 2011). In human blood cells, PCB 52 and PCB 133 induced transcriptional changes in several cytokine signaling and regulation pathways (Wens, De Boever et al. 2011). This effect was only observed in cells from male donors, suggesting a male-bias for some PCB effects. Another study showed that PCB 118 enhanced IL-4 producing T-cell development (Gaspar-Ramirez, Perez-Vazquez et al. 2012), which correlates with findings in children with ASD and their mothers (Gupta, Aggarwal et al. 1998; Abdallah, Larsen et al. 2011; Goines, Croen et al. 2011).
PCBs likely exert their immunotoxicity by interfering with immunologically-relevant signaling pathways. For example, dioxin-like PCBs interfere with Ahr signaling, which is critical for maintaining a healthy immune balance in the skin and gut (Li, Innocentin et al. 2011; Monteleone, Rizzo et al. 2011). PCBs also disrupt the key cytokine signaling pathways JAK/STAT and MAPK. Immune cells exposed to PCB 47 and 153 have compromised function and altered STAT 5 and ERK phosphorylation (Canesi, Ciacci et al. 2003). STAT 5 activation is central for regulatory T cell development (Burchill, Goetz et al. 2003; Adamson, Collins et al. 2009), and activation of this molecule by PCBs might mediate some of their immunosuppressive effects. Finally, some PCB congeners interfere with ryanodine receptors, which are expressed widely in the immune system. These receptors are regulated by cytokines including TGF-Β (Hosoi, Nishizaki et al. 2001; Pessah, Cherednichenko et al. 2010), and can induce IL-6 production after engangement (Treves, Vukcevic et al. 2011). In summary, PCBs alter immune function through a variety of mechanisms that may be relevant to immune profiles observed in autism.
PBDEs are a group of flame retardants that are the subject of growing concern due to their structural and functional similarities to PCBs. They are widely dispersed in the environment and bioaccumulate up the food chain (Johnson-Restrepo and Kannan 2009). Environmental PBDE levels have jumped dramatically in the last several decades, and are increasingly found in human tissues and breast milk (Noren and Meironyte 2000; Darnerud, Eriksen et al. 2001). Of note, this heightened prevalence is concurrent with the apparent rise in ASD diagnoses (Hertz-Picciotto and Delwiche 2009; Messer 2010). While not linked specifically to ASD, PBDE exposure is associated with improper neurodevelopment, hormonal disruptions, and a variety of behavioral, motor, and cognitive issues (Alm, Kultima et al. 2008; He, He et al. 2008; Roze, Meijer et al. 2009; Herbstman, Sjodin et al. 2010; Kodavanti, Coburn et al. 2010; Schreiber, Gassmann et al. 2010; Dingemans, van den Berg et al. 2011).
Some evidence shows that PBDEs can impact immune activity. Studies involving marine and murine models link PBDEs to changes in the immune system, including thymic and splenic atrophy, increased production of IL-10, lymphocyte depletion, reduced antibody recall responses, and decreased responses to pathogens (Fowles, Fairbrother et al. 1994; Thuvander 1999; Beineke, Siebert et al. 2005; Zhou, Chen et al. 2006; Beineke, Siebert et al. 2007; Beineke, Siebert et al. 2007; Lundgren, Darnerud et al. 2009; Watanabe, Shimizu et al. 2010; Bondy, Lefebvre et al. 2011; Fair, Stavros et al. 2012). There is little data regarding the impact of PBDEs on cytokine production and signaling, and future studies should examine these endpoints in more detail. One recent study considered the effect of BDE-47 on cytokine responses in children with autism and controls. Peripheral blood mononuclear cells obtained from ASD and typically developing controls were pretreated with PBDE-47 and stimulated with LPS. Analysis of supernatant cytokines showed that BDE-47 had a divergent impact on cells from ASD versus typical controls. Among controls, BDE-47 caused a significant decrease in inflammatory cytokines production (IL-6, IL-12, GM-CSF, TNF-α), indicating broad immune suppression. In contrast, children with ASD only down-regulated IL-6 in the presence of BDE-47, and had significantly higher IL-1Β responses (Ashwood, Schauer et al. 2009). This suggests that BDE-47 can enhance some aspects of inflammation in ASD. This diagnosis-specific immunotoxic effect suggests that children with ASD respond differently to PBDEs than typical children. Robust inflammation in response to such exposures during critical developmental windows could have long term neurological effects, and may be a possible mechanism in ASD. Future studies should continue to examine a potential role for PBDEs in immune dysfunction and neurodevelopmental disorders like autism.
Early-life infections can skew fetal development, leading to aberrant neural and immune activity. This is a widely suggested etiological mechanism in schizophrenia, and is increasingly implicated in autism as well (Patterson 2009). Several infections, including measles, cytomegalovirus, and rubella during preand perinatal periods have been associated with autism (Chess 1977; Markowitz 1983; Ivarsson, Bjerre et al. 1990; Sweeten, Posey et al. 2004; Libbey, Sweeten et al. 2005; Meyer, Feldon et al. 2011). A recent large-scale epidemiological study showed that infection-related hospitalizations during pregnancy significantly increased the risk of ASD (Atladottir, Thorsen et al. 2010). Interestingly, the risk was not associated with any specific type of infection, suggesting that the general immune mechanism controlling the response to the pathogen rather than the pathogen itself were involved. This response is likely / guaranteed to include cytokines. Indeed, there is clinical evidence for altered gestational cytokine milieus in autism (Abdallah, Larsen et al. 2011; Goines, Croen et al. 2011), which could be related to infectious exposures, and may mediate aspects of the disorder.
As discussed in previous sections, several studies have linked early immune challenges to long-term changes in behavioral and immune parameters. For example, mice prenatally exposed to influenza (Fatemi, Earle et al. 2002; Shi, Fatemi et al. 2003), the viral mimic Poly I:C (Vuillermot, Weber et al. 2010), or the bacterial component LPS (Romero, Guaza et al. 2010) demonstrate long term neurological and behavioral abnormalities. This effect is largely mediated by cytokines like IL-6 and IL-1Β (Samuelsson, Jennische et al. 2006; Smith, Li et al. 2007; Bilbo, Barrientos et al. 2008). Prenatal infectious exposures can also impact the developing immune system, and lead to long term immune dysregulation. This was demonstrated by increased levels of IL-6, IL-2, and TNF-α in adult animals that had been prenatally exposed to LPS (Romero, Guaza et al. 2010). Another interesting set of studies showed that an early life infection led to neurological deficits in adulthood that only became apparent after an immunological stimulation (Bilbo, Biedenkapp et al. 2005; Bilbo, Levkoff et al. 2005; Bilbo and Schwarz 2009). This suggests that early life infection can change immune responses later in life, and that this has neurological consequences. The concept that prenatal infections can impact both brain and immune development is intriguing, and should be explored further in autism.
Overall, when considering environmental exposures, it is important to take time to consider that the prevalence rates for ASD have increased dramatically over the last 10–20 years. These rates continue to increase year-on-year. Arguably concentrations for some compounds such as lead and chlorinated pesticides have fallen in the population since their removal or reduction from the environment in the 1970's. In contrast, levels of PBDEs and Ops have increased. Although it may be tempting to link compounds as risk factors for ASD based solely on similar time trends there is a need for more extensive research to begin to understand whether such temporal relationships are associated with risk for ASD. Future research should focus on the relationships between environmental exposures and risk for ASD diagnosis and whether environmental exposures to such compounds as PBDE induce cytokine responses that could modulate neuronal function in the pediatric population. Moreover, future research should discern whether children who develop a ASD are more sensitive to specific environmental exposures using cytokine production as readouts. More research focused on environmental exposures and ASD is warranted.
Cytokine imbalances are well documented in autism and have many interesting implications. Cytokines are intricately involved in neurodevelopment and neuronal function, and an ill-timed cytokine disruption can have long term neurological consequences. Further, cytokine expression is largely dependent on genetic and environmental influences. Therefore, they may represent a biomarker for genetic or environmental factors at play in autism. To illustrate the connection between immunity, genes, the environment, and neurodevelopmental outcome, consider two scenarios: First, an individual may be genetically poised to mount an inappropriate immune response to an infectious or toxic exposure. This individual might respond either too robustly or too weakly to resolve the threat without collateral damage to the brain and other body systems (including the immune system). Second, an individual may lack appropriate genetic machinery to excrete toxins; leading to their accumulation in tissue. This could lead to an amplification of the toxin's effects on a variety of body systems, including the brain and immune system. For each child, an environmental challenge during a critical window of development could have especially severe consequences, causing abnormal CNS function, altered immune phenotypes, and perhaps autism. These scenarios represent an emerging global view of autism that considers a broad contribution of several factors, including genes, the environment, and the immune system. Cross-disciplinary investigations that consider diverse biological contributions will be essential to untangle ASD.
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|By RealWire News Distribution
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|March 12, 2014 11:09 PM EDT
Manchester, UK - Worldwide thin client and cloud connection device manufacturer, VXL, unveils a new range of desktop devices for North America and Western Europe including two System-on-Chip (SoC) models, the Citrix ready, Xtona J12-z zero client & Gio-Linux J54 desktop together with VMware ready, Vtona V200, PCoIP® zero client with dual DVI.
Xtona J12-z, SoC, Citrix Ready™ Zero Client
Designed for the Citrix environment, the System-on-Chip J12-z incorporates the latest HDX™ Hardware Acceleration, a TI ARM 1.0GHz processor and an ultra-slim, compact desktop footprint. An environmentally friendly device, with a low power consumption of only 8.7 watts, the J12-z incorporates 4GB Flash / 1GB RAM onboard and a comprehensive feature set including four USBs, DVI-I / DV-D ports and Gigabit networking.
J54 Gio Linux, SoC, Desktop
The J54 System-on-Chip desktop also incorporates the latest TI ARM 1.0GHz processor and utilises VXL's Gio Linux operating system making it suitable for either VMware or Citrix user environments. Sharing the same ultra-slim design and low power consumption as the J12-z, the J54 also boasts the same impressive feature list including 4GB Flash / 1GB RAM onboard, four USBs, DVI-I / DV-D ports and Gigabit networking.
Vtona V200 VMware PCoIP® Zero Client
The perfect solution for VMware Horizon View™ users, the Vtona V200 Zero Client incorporates the Teradici 2321 PCoIP® processor for a superior performance for all Cloud, Virtualisation and Remote Desktop environments. Ensuring a rich multi-media performance including 3D graphics, CAD / CAM, video and more, the latest V200 also supports up to two displays with up to 2560 x 1600 resolution.
Classified as a 'true' zero client, the Vtona V200 has no operating system, no device drivers, no fan, no noise and comes complete with class-leading specification including 512MB memory, four USBs and a three year warranty as standard.
Frank Noon, VXL's VP Worldwide Sales commented "Our leadership position in the thin and cloud connection space reflects our ongoing strategy of developing innovative product solutions that meet our customer needs. These competitively priced models stay true to that concept, providing VMware or Citrix users with high performance, low power devices with excellent green credentials" he added.
- Ends -
Contact Ian Cope, Marketing Manager, VXL Instruments Ltd. Email: [email protected]
About VXL
Established in 1976, VXL is a global leader in thin client, cloud computing devices and has been manufacturing world-class thin client devices for over three decades providing a high quality range of high-specification models that are made to the most exacting standards.
This single-minded focus on thin clients and commitment to product quality is at the core of what VXL represents today together with a passion to support companies around the world to successfully build efficient IT infrastructures that are flexible, secure, manageable and affordable.
VXL's world-renowned 'Itona' brand of thin clients continues to go from strength to strength and includes its latest SoC (System-on-Chip) and dual / quad core high-performance desktops plus Xtona and Vtona zero clients, Lenovo Thin ThinkPad notebooks and leading software solutions including Fusion™ device manager and GioPC™ repurposing software.
VXL's corporate HQ and manufacturing is based in Bangalore, India's 'Silicon Valley', with the Americas Group HQ based in Houston, Texas and the European HQ in Manchester, UK. Dedicated Sales and Support offices are also based in key strategic markets including Germany, France and Singapore.
For more information please visit: www.vxl.net
Teradici and PCoIP are trademarks of Teradici Corporation and may be registered in the United States and/or other countries. Any other trademarks or registered trademarks mentioned in this release are the intellectual property of their respective owners.
VMware and VMware Horizon View are registered trademarks or trademarks (The "Marks") of VMware, Inc. in the United States and/or other jurisdictions.
Citrix, Citrix Ready, HDX, XenApp and XenDesktop are trademarks of Citrix Systems, Inc. and / or one or more of its subsidiaries in the United States Patent and Trademark Office and in other countries.
Source: RealWire
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"My role is working with customers, helping them go through this digital transformation. I spend a lot of time talking to banks, big industries, manufacturers working through how they are integrating and transforming their IT platforms and moving them forward," explained William Morrish, General Manager Product Sales at Interoute, in this SYS-CON.tv interview at 18th Cloud Expo, held June 7-9, 2016, at the Javits Center in New York City, NY.
Jul. 30, 2016 04:30 PM EDT Reads: 2,271
SYS-CON Events announced today that 910Telecom will exhibit at the 19th International Cloud Expo, which will take place on November 1–3, 2016, at the Santa Clara Convention Center in Santa Clara, CA. Housed in the classic Denver Gas & Electric Building, 910 15th St., 910Telecom is a carrier-neutral telecom hotel located in the heart of Denver. Adjacent to CenturyLink, AT&T, and Denver Main, 910Telecom offers connectivity to all major carriers, Internet service providers, Internet backbones and ...
Jul. 30, 2016 01:30 PM EDT Reads: 979
SYS-CON Events announced today that LeaseWeb USA, a cloud Infrastructure-as-a-Service (IaaS) provider, will exhibit at the 19th International Cloud Expo, which will take place on November 1–3, 2016, at the Santa Clara Convention Center in Santa Clara, CA. LeaseWeb is one of the world's largest hosting brands. The company helps customers define, develop and deploy IT infrastructure tailored to their exact business needs, by combining various kinds cloud solutions.
Jul. 30, 2016 11:30 AM EDT Reads: 1,409
For basic one-to-one voice or video calling solutions, WebRTC has proven to be a very powerful technology. Although WebRTC's core functionality is to provide secure, real-time p2p media streaming, leveraging native platform features and server-side components brings up new communication capabilities for web and native mobile applications, allowing for advanced multi-user use cases such as video broadcasting, conferencing, and media recording.
Jul. 30, 2016 10:45 AM EDT Reads: 1,125
SYS-CON Events announced today that Venafi, the Immune System for the Internet™ and the leading provider of Next Generation Trust Protection, will exhibit at @DevOpsSummit at 19th International Cloud Expo, which will take place on November 1–3, 2016, at the Santa Clara Convention Center in Santa Clara, CA. Venafi is the Immune System for the Internet™ that protects the foundation of all cybersecurity – cryptographic keys and digital certificates – so they can't be misused by bad guys in attacks...
Jul. 30, 2016 10:15 AM EDT Reads: 1,524
ReadyTalk has expanded the capabilities of the FoxDen collaboration platform announced late last year to include FoxDen Connect, an in-room video collaboration experience that launches with a single touch. With FoxDen Connect, users can now not only engage in HD video conferencing between iOS and Android mobile devices or Chrome browsers, but also set up in-person meeting rooms for video interactions. A host's mobile device automatically recognizes the presence of a meeting room via beacon tech...
Jul. 30, 2016 10:00 AM EDT Reads: 551
The cloud market growth today is largely in public clouds. While there is a lot of spend in IT departments in virtualization, these aren't yet translating into a true "cloud" experience within the enterprise. What is stopping the growth of the "private cloud" market? In his general session at 18th Cloud Expo, Nara Rajagopalan, CEO of Accelerite, explored the challenges in deploying, managing, and getting adoption for a private cloud within an enterprise. What are the key differences between wh...
Jul. 30, 2016 10:00 AM EDT Reads: 2,188
It's 2016: buildings are smart, connected and the IoT is fundamentally altering how control and operating systems work and speak to each other. Platforms across the enterprise are networked via inexpensive sensors to collect massive amounts of data for analytics, information management, and insights that can be used to continuously improve operations. In his session at @ThingsExpo, Brian Chemel, Co-Founder and CTO of Digital Lumens, will explore: The benefits sensor-networked systems bring to ...
Jul. 30, 2016 09:15 AM EDT Reads: 1,661
On Dice.com, the number of job postings asking for skill in Amazon Web Services increased 76 percent between June 2015 and June 2016. Salesforce.com saw its own skill mentions increase 37 percent, while DevOps and Cloud rose 35 percent and 28 percent, respectively. Even as they expand their presence in the cloud, companies are also looking for tech professionals who can manage projects, crunch data, and figure out how to make systems run more autonomously. Mentions of 'data science' as a skill ...
Jul. 30, 2016 09:00 AM EDT Reads: 626
Manufacturers are embracing the Industrial Internet the same way consumers are leveraging Fitbits – to improve overall health and wellness. Both can provide consistent measurement, visibility, and suggest performance improvements customized to help reach goals. Fitbit users can view real-time data and make adjustments to increase their activity. In his session at @ThingsExpo, Mark Bernardo Professional Services Leader, Americas, at GE Digital, discussed how leveraging the Industrial Internet a...
Jul. 30, 2016 08:00 AM EDT Reads: 797
Amazon has gradually rolled out parts of its IoT offerings in the last year, but these are just the tip of the iceberg. In addition to optimizing their back-end AWS offerings, Amazon is laying the ground work to be a major force in IoT – especially in the connected home and office. Amazon is extending its reach by building on its dominant Cloud IoT platform, its Dash Button strategy, recently announced Replenishment Services, the Echo/Alexa voice recognition control platform, the 6-7 strategic...
Jul. 30, 2016 07:45 AM EDT Reads: 737
In addition to all the benefits, IoT is also bringing new kind of customer experience challenges - cars that unlock themselves, thermostats turning houses into saunas and baby video monitors broadcasting over the internet. This list can only increase because while IoT services should be intuitive and simple to use, the delivery ecosystem is a myriad of potential problems as IoT explodes complexity. So finding a performance issue is like finding the proverbial needle in the haystack.
Jul. 30, 2016 07:45 AM EDT Reads: 2,426
There will be new vendors providing applications, middleware, and connected devices to support the thriving IoT ecosystem. This essentially means that electronic device manufacturers will also be in the software business. Many will be new to building embedded software or robust software. This creates an increased importance on software quality, particularly within the Industrial Internet of Things where business-critical applications are becoming dependent on products controlled by software. Qua...
Jul. 30, 2016 07:30 AM EDT Reads: 1,701
Big Data, cloud, analytics, contextual information, wearable tech, sensors, mobility, and WebRTC: together, these advances have created a perfect storm of technologies that are disrupting and transforming classic communications models and ecosystems. In his session at @ThingsExpo, Erik Perotti, Senior Manager of New Ventures on Plantronics' Innovation team, provided an overview of this technological shift, including associated business and consumer communications impacts, and opportunities it ...
Jul. 30, 2016 07:30 AM EDT Reads: 564
The 19th International Cloud Expo has announced that its Call for Papers is open. Cloud Expo, to be held November 1-3, 2016, at the Santa Clara Convention Center in Santa Clara, CA, brings together Cloud Computing, Big Data, Internet of Things, DevOps, Digital Transformation, Microservices and WebRTC to one location. With cloud computing driving a higher percentage of enterprise IT budgets every year, it becomes increasingly important to plant your flag in this fast-expanding business opportuni...
Jul. 30, 2016 05:15 AM EDT Reads: 2,728
IoT is rapidly changing the way enterprises are using data to improve business decision-making. In order to derive business value, organizations must unlock insights from the data gathered and then act on these. In their session at @ThingsExpo, Eric Hoffman, Vice President at EastBanc Technologies, and Peter Shashkin, Head of Development Department at EastBanc Technologies, discussed how one organization leveraged IoT, cloud technology and data analysis to improve customer experiences and effi...
Jul. 30, 2016 04:00 AM EDT Reads: 2,155


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SAN FRANCISCO (AP) - Banking into the wind and then gliding out of sight, a male California condor flew back into the wild after a captive breeding program that helped save North America's largest species of land bird.
LOS ANGELES (AP) - The mother of a fugitive Texas teen known for using an "affluenza" defense in a fatal drunken-driving accident will likely remain jailed for several days in Los Angeles after being deported from Mexico, investigators said Thursday.
SACRAMENTO (AP) - Amid the most destructive wildfire season in more than a decade, California's firefighting agency amped up its warnings to thousands of property owners who weren't doing enough to protect their homes by clearing brush and other flammable materials.
RIVERSIDE (AP) - A friend of one of the shooters in the San Bernardino massacre that killed 14 people was indicted Wednesday on charges that include conspiring in a pair of previous planned attacks and making false statements when he bought the guns used in this month's shootings, authorities said.
ECHO SUMMIT (AP) - The water content of the Sierra Nevada snowpack in drought-stricken California was 136 percent of normal Wednesday when officials took the winter's first manual survey - an encouraging result after nearly no snow was found at the site in April.
SACRAMENTO (AP) - The U.S. Department of Homeland Security granted a last-minute extension giving California more time to comply with the federal Real ID Act that sets stricter standards on identification.
SAN BERNARDINO (AP) - A moderate earthquake shook an inland area of Southern California near San Bernardino on Tuesday night, giving a start to thousands across a heavily populated area with more than one person comparing it to a rumbling big rig.
LOS ANGELES (AP) - A Los Angeles County jail has ended a policy of chaining inmates to a wall - sometimes for as long as 11 hours - until their bodies expel contraband.
NEW YORK (AP) - In the grand domain of splendid characters at Downton Abbey, Mr. Carson is perhaps the first among equals. Among the superb cast of "Downton Abbey," a similarly towering figure is Jim Carter, who plays him.
SAN DIEGO (AP) - A San Diego firefighter is challenging discipline he received after getting drunk at a department-sponsored training event, arguing that there is no city rule against it.
SAN FRANCISCO (AP) - Rescue crews in Northern California tranquilized a wayward seal that snarled traffic for two days by trying to cross a highway several times.
SAN FRANCISCO (AP) - Tired of paying nearly $4,000 a month for a one-bedroom in San Francisco's popular South of Market district, two newlyweds last year bought a townhouse in a budding development along the city's rough-edged southeastern bay-front.
SAN DIEGO (AP) - A man who was believed to have been distracted by an electronic device he was carrying has fatally fallen off a cliff above a San Diego beach.
TORRANCE (AP) - The Catalina Island fox, one of the world's most endangered species, suffered from frequent tumors for the past decade until scientists discovered a relatively simple solution: treating ear mites.
SAN FRANCISCO (AP) - Crystal Patterson didn't have the cash or assets to post $150,000 bail and get out of jail after her arrest for assault in October.
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COALINGA (AP) - An animal welfare organization says the state's last known gas chamber used to euthanize cats and dogs has been dismantled.
SAN FRANCISCO (AP) - The body of a girl found last month inside a small metal casket still holding a rose was laid to rest 145 ...
LOS ANGELES (AP) - Nearing the end of the primary season, a defiant Bernie Sanders predicted Saturday that the Democratic presidential process would lead to a ...
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July 27, 2011
By: Bill Frenzel and Tim Penny
It is time for our nation's leaders to step back from the brink. Obviously, neither side wants to blink first in this "stare down." However, both sides must stop focusing on political brinkmanship and instead look our fiscal challenges straight in the eye.
With the possible exception of a few House Republicans, all sides agree that failing to raise the debt limit will result in both fiscal and economic disruption — if not calamity. But addressing the debt limit without serious fiscal restraint is also risky — as rating agencies are threatening to downgrade our government's credit rating absent a clear and strong deficit reduction plan.
Unfortunately, despite the need for decisive action — our leaders seem incapable of the kind of compromise required when power is divided between the two major parties.
Set aside the hypocrisy exhibited by Democrats (including President Obama) who once opposed raising the debt ceiling during George Bush's White House tenure — and Republicans who now balk at a debt ceiling vote because Obama is in the Oval Office.
Forgive Obama, as well, for initially demanding a clean debt-ceiling increase — a nonstarter when faced with an "all cuts — no taxes" Republican House populated by freshmen elected on a platform of reducing our debt.
Forget, too, the Republican "cut, cap and balance" offer this past week — and recognize that votes of this sort are sometimes necessary to position legislators for a compromise later.
But now, the time for compromise has come. Compromise is not weakness. It is the way our government has been run for over 220 years. Like it or not, borrowing is necessitated whenever government spends more than it collects in revenue.
Accordingly, raising the debt ceiling is not optional. It is unavoidable.
Does that mean it needs to be done in a carte blanche fashion? No. In fact, during our time in Congress in the 1980s when — like today — there was divided government, Democrats in Congress and Republican President Ronald Reagan came together to enact budget controls (termed Gramm Rudman after the Senate authors of the proposal) in exchange for a debt-limit increase. Something comparable can and must be done today.
The Peterson-Pew Commission on Budget Reform (which we co-chair) has offered a budget-process reform that could be coupled with a debt-ceiling vote. If enacted, this process would control future spending and over time hold our nation's debt at manageable levels.
Another approach might be to allow for a small and short-term increase in the debt limit — perhaps enough to get us through to September — combined with a clear understanding that this interim period would be dedicated to enactment of an ambitious deficit-reduction package.
The recent $4 trillion proposal put forth by the Senate's bipartisan "gang of six" or the $3 trillion in savings evidently discussed by Speaker Boehner and President Obama might provide a framework for such a deal. Both plans represent the type of principled compromise in which both parties are willing to accept things they don't like for the good of the nation.
Much is at stake with a fiscal cliff fast approaching. Our nation's leaders need to find a way to compromise without compromising their principles, just as President Reagan and Democrats did in the 1980s.
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Rep. Welch touring southern VermontMarch 31,2014Rep. Peter Welch will be busy in southern Vermont today.
The congressman will host a meeting at 9:30 a.m. at 122 Birge St. in Brattleboro on energy efficiency with community members, contractors and energy efficiency leaders.
Later, at noon, he will speak to the Springfield Rotary Club at The Hartness House in Springfield.MORE IN This Just InMore than 26,000 people were without power when severe thunderstorms rolled through the state in... Full Story
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