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post-tetanic potentiation
- PTPEPSC and PTPmEPSC
EPSC and mEPSC amplitude potentiation
- PTPEPSC_fast and slow
fast and slow component of the PTPEPSC
- Train10s
train of stimuli at 100 Hz for 10 s
Post-tetanic potentiation is caused by two signalling mechanisms affecting quantal size and quantal content
Corresponding author L.-G. Wu: National Institute of Neurological Disorders and Stroke, 35 Convent Drive, Bldg 35, Bethesda, MD 20892, USA. Email: email@example.com
A high-frequency action potential train induces post-tetanic potentiation (PTP) of transmission at many synapses by increasing the intra-terminal calcium concentration, which may increase the quantal content by activation of protein kinase C (PKC). A recent study found that an increase of the mEPSC size, caused by compound vesicle fusion, parallels PTP, suggesting that the quantal size increase also contributes to the PTP generation. However, the strength of this suggestion is somewhat undermined by recent studies suggesting that vesicles responsible for spontaneous and evoked EPSCs may originate from different pools. Furthermore, it is unclear whether the quantal size increase is also mediated by PKC. The present work addressed these issues at a large calyx of Held synapse. We found that PTP was caused by both a PKC-dependent increase of the quantal content and a PKC-independent increase of the quantal size. In addition, we found that mEPSCs and EPSCs were subjected to similar up- and down-regulation, which verifies the basic assumption of quantal analysis – the same mechanism controls the quantal size of spontaneous and evoked release. This verification supports the use of quantal analysis at central synapses. However, unlike the traditional quantal analysis that attributes the quantal size change to a postsynaptic mechanism, the present work, together with one of our previous studies, suggests that the quantal size increase is caused by a presynaptic mechanism, the compound fusion among vesicles that forms large compound vesicles.
Synaptic plasticity is critical in the neuronal circuit function, neuronal development, and certain forms of learning and memory (Zucker & Regehr, 2002; Abbott & Regehr, 2004; Malenka & Bear, 2004). A high-frequency train of action potential stimulation may induce post-tetanic potentiation (PTP) of synaptic transmission for a few minutes at many synapses (Zucker & Regehr, 2002). Accumulated evidence suggests that this form of synaptic plasticity is mainly caused by a calcium-dependent activation of protein kinase C (PKC), which increases the number of released vesicles (quantal content) by increasing the release probability and/or the readily releasable pool size (Alle et al. 2001; Brager et al. 2003; Habets & Borst, 2005, 2006; Korogod et al. 2005, 2007; Xu et al. 2007).
A recent study showed that PTP of the evoked, AMPA receptor-mediated EPSC was accompanied by calcium-dependent potentiation of the mEPSC amplitude at the calyx of Held synapse (He et al. 2009). The mEPSC amplitude potentiation was smaller than the PTP in the first minute after tetanic stimulation, but became similar to the PTP ∼1 min later, suggesting that in addition to the increased vesicle number, an increase in the quantal size contributes to the generation of PTP (He et al. 2009). However, two issues remained unresolved. First, it is unclear whether the calcium-dependent quantal size increase is also caused by activation of PKC in a similar way as the calcium-dependent increase of the quantal content. Second, recent studies suggest that spontaneous and evoked release originate from different vesicle pools at hippocampal synapses (Sara et al. 2005; Atasoy et al. 2008; Mathew et al. 2008; Fredj & Burrone, 2009; Chung et al. 2010; but see Groemer & Klingauf, 2007). These studies suggested caution in using quantal analysis, a traditional analysis of evoked release based on the assumption that the spontaneous quantal event is the same as the quantal event during evoked release (Zucker, 2005; Rothwell, 2010). Consequently, additional evidence may be needed to strengthen the suggestion that quantal size increase, detected as an increase of the mEPSC amplitude, contributes to the generation of PTP.
The present work addressed the above two issues by recording both the mEPSCs and EPSCs at a large central synapse, the calyx of Held synapse. We found that PTP of the EPSC is caused by two different cellular mechanisms, a PKC-dependent increase of the quantal content and a PKC-independent increase of the quantal size. In addition, our study further supports the use of quantal analysis at central synapses.
Wistar rats 7–10 days old were rapidly decapitated (Borst et al. 1995; Sun & Wu, 2001). Horizontal brainstem slices (200 μm thick) containing the medial nucleus of the trapezoid body were prepared using a vibratome (He et al. 2006). All animal protocols followed the guidelines of the National Institutes of Health, USA, and complied with The Journal of Physiology policy and UK regulations on animal experimentation (Drummond, 2009). Slices were incubated for 30 min at 37°C and then held at room temperature (22–24°C) for experiments in a solution containing (in mm): 125 NaCl, 2.5 KCl, 1 MgCl2, 2 CaCl2, 25 dextrose, 1.25 NaH2PO4, 0.4 ascorbic acid, 3 myo-inositol, 2 sodium pyruvate, 25 NaHCO3, pH 7.4 when bubbled with 95% O2–5% CO2. A bipolar electrode was placed at the midline of the trapezoid body. A 0.1 ms, 2–20 V voltage pulse was applied to this electrode to evoke an action potential at the axon and calyx, which induced an AMPA receptor-mediated EPSC at the principal neuron of the medial nucleus of the trapezoid body. The principal neuron was selected for recording when an action potential, as detected with an extracellular electrode, was evoked by the axonal stimulation (Borst et al. 1995). Voltage-clamp recordings of AMPA receptor-mediated EPSCs and mEPSCs were performed with an EPC 10 amplifier (HEKA, Lambrecht, Germany) using pipettes (2–3 MΩ) containing (in mm): 125 potassium gluconate, 20 KCl, 4 MgATP, 10 Na2-phosphocreatine, 0.3 GTP, 10 Hepes and 0.5 EGTA, pH 7.2, adjusted with KOH (Xu & Wu, 2005). The series resistance (<10 MΩ) was compensated by 85–95% throughout the experiment, including during recordings of the EPSC and the mEPSC. The control bath solution contained (in mm): 125 NaCl, 2.5 KCl, 1 MgCl2, 2 CaCl2, 25 dextrose, 1.25 NaH2PO4, 0.4 ascorbic acid, 3 myo-inositol, 2 sodium pyruvate, 25 NaHCO3, 0.05 d-APV, 0.01 bicuculline and 0.01 strychnine, pH 7.4 when bubbled with 95% O2–5% CO2. Bisindolylmaleimide I (BIS) and Ro31-8220 were purchased from CalBiochem (San Diego, CA, USA). As in our previous study (He et al. 2009), the mEPSC was analysed with a mini program (Mini Analysis Program, Synaptosoft Inc., NJ, USA). The statistical test used is t test, if not mentioned otherwise. The data were expressed as mean ±s.e.m.
Figures 1–4 show sampled EPSCs and mEPSCs with a half-width differing by 2–3 times. The synapse heterogeneity and the age difference of the synapse may account for this difference. It has been shown that the half-width of the EPSC and mEPSC decreased by nearly 3 times on average as the calyx of Held synapse matures from post-natal day (P) 5–7 to P12–14 (Taschenberger & Von Gersdorff, 2000; Joshi & Wang, 2002), owing to a speeding up of AMPA receptor deactivation and desensitization (Koike-Tani et al. 2005). Our data were taken from 7- to 10-day-old rats, in which EPSCs and mEPSCs from 7-day-old rats should be slower than those from 10-day-old rats, on average.
PKC-independent potentiation of the mEPSC size and the EPSC are similar in amplitude and time course
A brief stimulus (0.1 ms, 2–20 V) was applied every 10 s via a bipolar electrode positioned at the midline of the trapezoid body, which evoked an action potential at the calyx and thus an EPSC as recorded from the postsynaptic neuron (Fig. 1A) (Borst et al. 1995). While the evoked EPSC was recorded, the mEPSC was collected continuously. Both the EPSC and the mEPSC originated from the same calyx of Held nerve terminal (Von Gersdorff & Borst, 2002). After establishing a stable baseline, a train of stimuli at 100 Hz for 10 s (Train10s) was applied, which induced a PTP of the EPSC (PTPEPSC) (Habets & Borst, 2005; Korogod et al. 2005) and an increase of the mEPSC amplitude (Fig. 1A, n= 6) (He et al. 2009). During Train10s, the EPSC was depressed (Fig. 1B) mostly due to depletion of the readily releasable pool (Von Gersdorff et al. 1997; Wu & Borst, 1999; Schneggenburger et al. 1999; Xu & Wu, 2005). As measured 10–30 s after Train10s, the EPSC amplitude was above the baseline and reach the peak (Fig. 1A, see also Fig. 2A and Fig. 3A), indicating that short-term depression fully recovered within 30 s. The mEPSC amplitude potentiation was observed immediately after Train10s (Fig. 1A, see Fig. 1B for sample raw data).
Similar to our previous report (He et al. 2009), the decay of the PTP of the EPSC amplitude could be fitted with a bi-exponential function with time constants of 40 s, and 203 s, respectively (Fig. 1A). The amplitude for the fast component of the PTPEPSC (PTPEPSC_fast) was 62% as compared to the baseline EPSC amplitude (note that the data were normalized to the baseline before tetanic stimulus), whereas the amplitude of the slow component (PTPEPSC_slow) was 41%. The mEPSC amplitude potentiation (PTPmEPSC) decayed approximately mono-exponentially with an amplitude (39 ± 4%, n= 6) and a time constant (212 s) similar to those of PTPEPSC_slow (Fig. 1A). These results were similar to our previous report (He et al. 2009). Although these results were obtained in P7–10 rats, similar results were obtained in P13–14 rats (n= 3) (He et al. 2009). We also noticed that Train10s induced a transient increase of the mEPSC frequency (Fig. 1A–B), consistent with the finding that the PTPEPSC_fast results from an increase of the released vesicle number (Korogod et al. 2005; Habets & Borst, 2005).
It has been shown that two PKC inhibitors, bisindolylmaleimide I (BIS) and Ro31-8220, did not inhibit the baseline EPSC, but blocked the generation of the PTPEPSC induced by a 4 s stimulation at 100 Hz (Korogod et al. 2007). To determine whether PKC inhibitors block both the PTPEPSC and the PTPmEPSC induced by Train10s, BIS and Ro31-8220 were separately applied to the bath. The effect of BIS was evident within 5 min of BIS application, which allowed us to compare the PTP results before and after BIS application from the same cell. At ∼5 min after BIS (10 μm) application, Train10s induced a PTPEPSC and a PTPmEPSC, both of which decayed mono-exponentially (Fig. 1C). The amplitude of the PTPEPSC (40 ± 11%, n= 6, Fig. 1C) was much smaller than the control before BIS application (104 ± 7%, n= 6, Fig. 1A, P < 0.01). The PTPEPSC decayed mono-exponentially with a time constant (239 s, Fig. 1C) similar to the PTPEPSC_slow in control (203 s, Fig. 1A). In contrast to the partial block of the PTPEPSC, the PTPmEPSC amplitude (34 ± 5%, n= 6, Fig. 1C) and time constant (193 s) were similar to control (amplitude: 39 ± 4%, n= 6, P > 0.2; τ= 212 s, Fig. 1A). Clearly, in the presence of BIS, the PTPmEPSC amplitude and time constant were similar to the PTPEPSC amplitude (40 ± 11%, n= 6, P > 0.1) and time constant (239 s, Fig. 1C). The mEPSC frequency increase induced by Train10s in the presence of BIS (Fig. 1C) was similar to that of control (Fig. 1A), consistent with the previous report (Korogod et al. 2007).
Similar results were obtained for another PKC inhibitor, Ro31-8220 (Fig. 2A and B, n= 5). Since the effect of Ro31-8220 was evident only after ∼30 min application, we obtained the control data (Fig. 2A) from different cells to those subjected to Ro31-8220 application (Fig. 2B). In summary, the PTPEPSC_fast was sensitive to PKC inhibitors, whereas the PTPEPSC_slow and the PTPmEPSC, both of which had a similar amplitude and time constant, were not (Figs 1 and 2). These results suggest that PTPmEPSC was PKC independent and was responsible for the generation of the PKC-independent PTPEPSC_slow.
A change in PTPmEPSC is accompanied by a similar extent of change in PTPEPSC
The use of quantal analysis to study synaptic plasticity has been challenged by recent studies suggesting that spontaneous and evoked release may originate from different vesicle pools at hippocampal synapses (Sara et al. 2005; Atasoy et al. 2008; Mathew et al. 2008; Fredj & Burrone, 2009; Chung et al. 2010; but see Groemer & Klingauf, 2007). Accordingly, the possibility that the similar amplitude and time course of PTPmEPSC and the PTPEPSC_slow is due to co-incidence rather than a causal relation could not be fully excluded. A causal relation between PTPmEPSC and PTPEPSC_slow would predict that a decrease or an increase in PTPmEPSC should cause a proportional change in PTPEPSC. We tested this prediction by either decreasing or increasing PTPmEPSC in the presence of BIS, which blocked PTPEPSC_fast and thus isolated PTPEPSC_slow (Fig. 1).
Reduction of PTPmEPSC was made by decreasing the intensity of the tetanic stimulus from 10 s to 4 s at 100 Hz (Train4s), which induced a much smaller PTPmEPSC (16 ± 6%, n= 5, Fig. 3A) than Train10s in the control condition (He et al. 2009). Train4s induced a PTPEPSC with an amplitude of 57 ± 21% (n= 5), the decay of which was fitted bi-exponentially with time constants of 43 s (44%) and 183 s (13%), respectively (Fig. 3A). The time constant and amplitude of PTPEPSC_slow were similar to those of PTPmEPSC. In the presence of BIS (10 μm), PTPEPSC_fast was abolished (Fig. 3B). The remaining PTPEPSC, PTPEPSC_slow, had a small amplitude (18 ± 7%, n= 5) and a mono-exponential time constant (201 s) similar to those of PTPmEPSC (12 ± 3%, n= 5, τ= 182 s, Fig. 3B). Evidently, in the presence of BIS, reducing the tetanic stimulus intensity from Train10s to Train4s reduced PTPmEPSC and PTPEPSC to a similar extent (compare Fig. 3B with Fig. 1B). This result strengthens the suggestion that PTPmEPSC is responsible for PTPEPSC_slow.
The increase of PTPmEPSC was made by bath application of a competitive AMPA receptor antagonist, γ-DGG (300–600 μm), which relieves AMPA receptor saturation by blocking AMPA receptors to a lesser degree at higher glutamate concentrations (Liu et al. 1999). If PTPmEPSC was due to an increase of the transmitter amount in a vesicle as suggested recently (He et al. 2009), AMPA receptors would be more saturated during PTPmEPSC. Accordingly, γ-DGG should relieve AMPA receptor saturation and thus increase PTPmEPSC. Indeed, in the presence of BIS (10 μm) and γ-DGG (300–600 μm in the bath), the latter of which decreased the baseline mEPSC and EPSC amplitude by ∼24–36% (n= 7, not shown), Train10s induced a PTPmEPSC with an amplitude of 55 ± 5% (n= 7, Fig. 4A) significantly higher than that in the absence of γ-DGG (but in the presence of BIS, 34 ± 5%, n= 6, Fig. 4B, P < 0.01). The PTPEPSC amplitude was 61 ± 10% (n= 7, Fig. 4A), which was similar to PTPmEPSC (Fig. 4A), but significantly higher than PTPEPSC in the absence of γ-DGG (40 ± 11%, P < 0.01, n= 6, Fig. 4B).
As a control, we also examined the effect of a non-competitive AMPA receptor blocker, NBQX. NBQX at 60–80 nm reduced the baseline mEPSC and EPSC by a similar percentage (∼26–40%, n= 6, not shown) as γ-DGG at 300–600 μm, but did not increase either PTPmEPSC or PTPEPSC. In the presence of NBQX and BIS, Train10s induced a PTPmEPSC (32 ± 3%, n= 6) and a PTPEPSC (36 ± 7%, n= 6, Fig. 4C) similar to those in the presence of only BIS (Fig. 4B, P > 0.3). This result suggests that the increase of PTPmEPSC and PTPEPSC by γ-DGG (Fig. 4A) is not caused by a decrease of the mEPSC amplitude itself, but by an increase of the transmitter amount. The similar increase of PTPmEPSC and PTPEPSC in the presence of γ-DGG (Fig. 4), and the similar decrease of both of them when Train10s was changed to Train4s (Fig. 3), suggested that PTPmEPSC is responsible for the generation of PTPEPSC.
A presynaptic quantal size increase contributes to the generation of PTP
PTP can be divided into a fast (PTPEPSC_fast) and a slow (PTPEPSC_slow) component (Fig. 1A) (He et al. 2009). A previous study suggests that compound fusion between vesicles is responsible for PTPmEPSC and thus PTPEPSC_slow (He et al. 2009). However, this suggestion needs to be strengthened because the use of quantal analysis has been challenged owing to the observation that spontaneous and evoked release may originate from different vesicle pools (Zucker, 2005; Rothwell, 2010). The current work has strengthened this suggestion by presenting two additional pieces of evidence at the calyx of Held in 7- to 14-day-old rats. First, both the PTPEPSC_slow and PTPmEPSC were resistant to PKC inhibitors BIS and Ro31-8220, whereas PTPEPSC_fast was not (Figs 1 and 2). When PTPEPSC_fast was abolished by PKC inhibitors, PTPEPSC_slow and PTPmEPSC were similar in both amplitude and time course (Figs 1 and 2). Second, both PTPEPSC_slow and PTPmEPSC were reduced to a similar extent when the intensity of the PTP-inducing stimulus was reduced (Fig. 3), and were increased to a similar level during γ-DGG application (Fig. 4). Since γ-DGG is a competitive AMPA receptor blocker that can relieve AMPA receptor saturation, the increase of PTPmEPSC by γ-DGG (Fig. 4) suggests that PTPmEPSC is due to an increase in the glutamate concentration at the synaptic cleft, consistent with the finding that compound fusion causes an increase in the mEPSC size (He et al. 2009). These results significantly strengthen the suggestion that PTPmEPSC is responsible for the generation of PTPEPSC_slow. Together with the previous observation that calcium/synaptotagmin-mediated compound fusion between vesicles occurs during PTP (He et al. 2009), our results suggest that compound fusion increases the vesicle size and transmitter amount, which mediates PTPmEPSC and thus PTPEPSC_slow.
PTP is caused by PKC inhibitor-sensitive and -insensitive mechanisms
Previous studies suggest that PTP, including that induced by Train4s at the calyx, is mediated by a PKC inhibitor-sensitive increase of the quantal content (Alle et al. 2001; Brager et al. 2003; Korogod et al. 2007). The present work confirms this observation, and revealed an additional mechanism, the PKC inhibitor-resistant increase of the quantal size. This additional mechanism, PTPmESPC, was not reported in previous studies at the calyx (Korogod et al. 2007, 2005; Habets & Borst, 2005, 2006). The reason for this discrepancy is mostly due to the difference in the intensity of tetanic stimulation used to induce PTP. We used mostly 100 Hz stimulation for 10 s, whereas previous studies used 100 Hz stimulation for 4 s or a lower frequency of stimulation. When we reduced the tetanic stimulation from 10 to 4 s at 100 Hz, PTPmEPSC was as low as 12 ± 3% (n= 5, Fig. 3B). This minimal increase of PTPmEPSC was in general consistent with previous studies showing that PTP induced by the 100 Hz stimulation for 4 s or a lower frequency of stimulation is mainly not caused by the quantal size increase at the calyx preparation (Habets & Borst, 2005, 2006; Korogod et al. 2005, 2007). The slight difference (between no PTPmEPSC and ∼12% PTPmEPSC) is understandable, because the focus of the studies is different; and the slice condition, the selection of the synapse for recording and the data analysis performed by different labs cannot be exactly the same.
The present work showed that PTP is mediated by two mechanisms, a PKC inhibitor-sensitive fast component owing to an increase of the quantal content, and a PKC inhibitor-insensitive slow component owing to an increase of the quantal size. Both mechanisms are initiated by calcium influx, because the calcium buffer EGTA abolished both PTPESPC (Habets & Borst, 2005; Korogod et al. 2005) and PTPmESPC (He et al. 2009). The increase of the released vesicle number for the PKC inhibitor-sensitive fast component is due to an increased sensitivity of the release machinery to calcium, an increased calcium influx during an action potential and/or an increased readily releasable pool size (Korogod et al. 2005; Habets & Borst, 2006, 2007).
At the calyx of Held in 7- to 10-day-old rats, a high-frequency train of action potential stimulation causes depletion of the readily releasable pool and inactivation of calcium currents after the stimulation, which causes subsequent short-term depression of release for about 10–20 s (Von Gersdorff et al. 1997; Wu & Borst, 1999; Schneggenburger et al. 1999; Xu & Wu, 2005). Replenishment of the readily releasable pool with vesicles having a lower release probability (reluctant vesicles), conversion of reluctant vesicles to regular vesicles, and recovery of the calcium current inactivation contribute to the recovery of short-term depression (Wu & Borst, 1999; Wadel et al. 2007). The present work did not focus on the first 10–20 s after the PTP-inducing stimulus, the Train10s or Train4s. However, we noticed that PTPEPSC reached a peak within 20–30 s after Train10s or Train4s (Figs 1A, 2A and 3A). Thus, within the first 20–30 s after the PTP-inducing stimulus, the mechanisms that cause PTP and short-term depression must take place simultaneously. The EPSC within this time period may thus reflect the net change between PTP and short-term depression of the EPSC.
A recent study at the calyx showed that MLCK inhibitors largely blocked PTP induced by Train4s, suggesting the involvement of MLCK (Lee et al. 2008). This study also showed that a BIS analogue that could not inhibit PKC partly mimics BIS in blocking PTP, suggesting that at least part of the PTP fast component is not mediated by the PKC signalling pathway. Distinguishing whether MLCK or PKC is involved in the PTP fast component is not the focus of the present work. Rather, the present work aimed at determining whether the PTP slow component is mediated by a mechanism different from the PTP fast component. BIS and Ro31-8220 served well for this purpose by blocking only the fast, but not the slow, component of the PTP. We therefore cautiously concluded that the PTP fast component is PKC inhibitor sensitive, which may involve PKC and/or MLCK, whereas the PTP slow component is not.
Quantal analysis at central synapses
The traditional quantal analysis often interprets the quantal size change as the postsynaptic change. The present work, together with our previous work (He et al. 2009), suggests that the quantal size change during synaptic plasticity may also reflect a presynaptic change.
Recent studies at cultured hippocampal synapses suggest that spontaneous and evoked release originate from different vesicle pools (Sara et al. 2005; Atasoy et al. 2008; Mathew et al. 2008; Fredj & Burrone, 2009; Chung et al. 2010; but see Groemer & Klingauf, 2007). These studies suggest caution in the use of quantal analysis, a tool that has been used to analyse the evoked release for many decades, based on the assumption that the quantal size of the spontaneous and evoked release is the same (Zucker, 2005; Rothwell, 2010). The present work showed that the mEPSC size potentiation paralleled the PTP slow component in amplitude and time course in various conditions that decreased or increased the PTP amplitude. The parallel changes of the mEPSC and the EPSC size do not necessarily exclude the possibility that spontaneous and evoked release come from different vesicle pools, but do suggest that the same mechanism controls the quantal size of spontaneous and evoked release. This suggestion supports the assumption of quantal analysis that the mEPSC size reflects the quantal size during evoked release. Thus, regardless of the origin of spontaneous and evoked release, quantal analysis can still be used to study changes in synaptic strength at central synapses, at least at the calyx of Held synapse.
Author contributions
L.X. designed and performed experiments and analysed data. L.-G.W. designed experiments and wrote the paper. Both authors approved the final version.
We thank Drs Liming He and Fujun Luo for comments on the manuscript. This work was supported by the National Institute of Neurological Disorders and Stroke Intramural Research Program.


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I am developing an app that utilizes two different uploaders. What I am trying to do is to assign each uploader to different cloudfiles containers. I seem to unable to find any documentation about this, could anyone please help? Thanks!!
Announcing Stack Overflow Documentation
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Basically you have to override the

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Fun activities for Easter Weekend
Easter Weekend in Montréal is a veritable fountain of fun and surprises. To usher in this sweet spring holiday,...Read more
Montréal is known for its lively Village scene, where LGBTQ locals and travellers congregate for cruising and schmoozing. But there's more to us than parties and nightlife.
Montréal is also about long walks in the park, romantic strolls down cobblestone streets, relaxing at an outdoor café, discovering unique finds in trendy boutiques, dancing in the streets at outdoor festivals, and sharing amazing meals.
And when the time comes, you can introduce us to your family, with a host of fun, kid-friendly things to do and places to see.
So let's get to know each other better, and see where it leads us.
Easter Weekend in Montréal is a veritable fountain of fun and surprises. To usher in this sweet spring holiday,...Read more
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We'll be missing the fun frenzy of Igloofest after this weekend, when the 2015 edition comes to a close, that's...Read more
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→ Select your interests
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SOUTH JORDAN, Utah — Broadway leading lady and Valley Music Hall star Melva Niles Barborka died on March 12.
The South Jordan resident was 86 and died of chronic inflammatory demyelinating polyneuropathy, a neurological disease.
In 1948, Niles Barborka created the role of Solis in the Broadway premiere of "Magdalena," composed by one of Brazil's most famous composers, Heitor Villa-Lobos. She also starred in the Broadway production of "Song of Norway."
At the popular musical theater production company Valley Music Hall in Bountiful, Utah, Niles Barborka performed opposite Robert Peterson in "Kiss Me, Kate," James Shigeta in "The King and I" and Art Lund in "I Do, I Do."
Niles Barborka also starred in the Los Angeles Civic Light Opera productions of "Magdalena" and "Song of Norway." Edwin Lester, the company's longtime producer and founder, said she had "the most beautiful soprano voice I have ever heard." In Los Angeles, she also performed opposite Mitzi Gaynor and Walter Slezak in 1949's "The Great Waltz."
With Peterson, Niles Barborka recorded a collection of LDS hymns, "Things Lovely," with the CBS Radio Orchestra. Two other recordings were "Bring Us Together," a 1968 concert tour with Peterson, and "The Song of Norway" from the 1949 Rail Road Hour production, opposite Gordon MacRae.
During the national tour of "Song of Norway," she met Clifford Joseph Barborka Jr., whom she married in June 1949. The couple's conversion to The Church of Jesus Christ of Latter-day Saints is recorded in "No More Strangers, Volume I" by Hartman Rector Jr.
With her husband, Niles Barborka made many public appearances to speak about the LDS Church following her retirement from the stage.
Niles Barborka is survived by two sons, Utah residents Cliff Joseph Barborka III and Vincent Barborka.
According to her obituary, "Melva Niles Barborka had a magical way of connecting with people. Whether it was a waitress at a roadside cafe, a child in need or a leader of her beloved church, she had the ability to make everyone she met feel like they were the most important person in the world."
Funeral services will be conducted on Monday, March 19, at noon in the South Jordan Stake Center at 10400 S. 2450 West in South Jordan. A viewing will be held in the same building on Sunday, March 18, from 6 p.m. to 9 p.m., and prior to Monday's services from 9:30 to 11:45 a.m. Interment will be at Veteran's Memorial Cemetery.
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We eat here a lot. I know we are going to get a good meal when we come here. Drinks are reasonably priced. I usually get the filet and scallops.
I've eaten at Grinnell's for many years. It has been one of my favorite go-to places for special occasions for many reasons. Excellent menu, delicious food, excellent service and wonderful atmosphere when celebrating special occasions.
As often as I have eaten at Grinnell's, I have never written a review, until now. If I had written reviews of all of past...
Thank you so very much for the thorough and thoughtful review. It was our absolute pleasure to host you and your girlfriend, and the fact that she enjoys ordering meat at our spot is a testament to our dedication, and we're so thankful to learn that she loves our lamb chops.
Thank for you choosing us for your special night,...
I had the filet and my wife had scallops. Both were out of this world. We started with the grilled butterfly jumbo shrimp---they are so big and delicious that they look like small lobsters! We have been customers of Grinnells for more than 4 decades, and we have never been disappointed. The family operation offers attention to detail that is...
John, four decades? That is absolutely amazing. We're are flattered, humbled, and very grateful to have been a part of your life for so long -- thank you. It seems that you are a member of our family at this point and we will always be so happy to serve you up some steak and scallops whenever the time feels...
Dining at Grinnell's is a step back in time to where and when dining was more than a meal. The menu is just one indication of the quality of the preparation and the food itself. It reads like a great steak and chop house menu with no frills; just great choices of outstanding selections of top quality meat. Appetizers and...
Nancy, this review is absolutely amazing. We're so glad to have had you in, and even more glad to read this wonderful testament. We do our best to provide the exact dining experience you wrote about -- there isn't much that can beat top-quality cuts of meat cooked just the way you like it.
We hope to have you back....
My wife and I go to Grinnell's 4-5 time a year when we want that special dinner with special service. From the minute you walk in the door you know they are going to take good care of you. There are plenty of excellent restaurants in Rochester and the surrounding area, but when we want something special, we make the...
We can't express to you the joy we feel reading this review. It's absolutely stunning and fills us with joy. Thank you for taking the time to describe your relationship with our restaurant. Having you in 4-5 times a year is an absolute delight, and we're looking forward to it for years to come.
We care about the food, but...
Went with a group of 12 people. The small bar area was okay to get a before dinner drink. Bartender made very good drinks but service was a tad slow. One bartender taking care of both the dining room and those at the bar was a bit of a challenge but he managed.
Got seated for dinner and the service...
Paul, while it is fantastic to see how much you enjoyed the serivce, we're greatly concerned about your complaints about the food. There is no excuse for the meals not coming out the way you ordered them, and we hope you will accept our sincere apology for that.
With that being said, we want to express to you our absolute...
We have gone to this restaurant many times and the food and service is consistently great. Staff go out of their way to provide great service and with attention to every detail. The dry aged beef is especially good and of excellent quality. The drinks are cold and the price fair!
Thank you for thinking so highly of us, Kathy -- knowing you enjoy consistent five-star meals and service with us is a compliment like no other! All of us love going out of our way to make sure you have the best experience possible.
See you soon for more of that dry aged beef you love so much, and of...
This place reminded me of the classic New York City chop houses. While they seem to specialize in chops, steaks and prime rib, there are many other options on the menu. The waitstaff is attentive, the owner came by several times to see if we were happy, everyone in our party of 7 loved the meal and the atmosphere is...
It's true -- this restaurant is our pride and joy, and we're so happy it shows! Thank you for the wonderful review, Rob. It's great to hear that you're a fan of our food, service, and atmosphere. We couldn't ask for more. Be sure to visit us again soon.
All the best,
Dave & Matt - Owners
One of our favorite places for birthdays and anniversaries. The valet parking (required) is good and friendly. Wonderful service, pleasant atmosphere, and outstanding menu. We are never disappointed and leave saying "We need to come here more often!"
Ken, it's so great to see you're a fan! Thanks so much for letting us know. Now, we look forward to seeing you again soon.
All the best,
Dave & Matt - Owners
First time visit. Went for the prime rib which was properly prepared to order and very tasty. Pleasantly surprised by Two Hearted Ale on tap, one of our favorites. Ambiance is comfortable and cozy. Background music just loud enough to mask conversations from neighboring tables. Service was timely and unobtrusive. The only thing to find any fault with was the...
Thanks for giving us a shot -- it sure was wonderful to have you over! From the vibe, to our brews, to your meal, we're delighted to know you enjoyed it all. Sorry about your water, but please know that your comments will not go unnoticed. Your satisfaction is our number one priority, and we sure hope we'll be welcoming...
Own or manage this property? Claim your listing for free to respond to reviews, update your profile and much more.
are there any pictures of the location of the " junior suite penthouse rooms"
there was no such thing before they did the renovations. we are looking at the
site to go in june.
See all questions


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This property allows you to book online with a credit card. After submitting a reservation request, the owner or manager has 24 hours to accept, at which time your card will be charged and you will receive an email confirmation
7 BR 5.5 BA Sleeps 21$660 avg/night4-7 night min stay
2 BR 2 BA Sleeps 6$94 avg/night3-7 night min stay
2 BR 2 BA Sleeps 7$144 avg/night3-7 night min stay
3 BR 3 BA Sleeps 8$229 avg/night3-6 night min stay
3 BR 3 BA Sleeps 8$227 avg/night3-7 night min stay
2 BR 1.5 BA Sleeps 8$109 avg/night4-5 night min stay
Recent Mid-Destin reviews
"A great vacation is only made possible by a great location and for your vacation in Destin this Sterling Shores condo has that and much more! It is centrally located to any attraction your heart desires - whether it be the great restaurants, shopping, HarborWalk Village, deep sea fishing. etc or most importantly, the great white sands of the Gulf! We enjoyed 10 days just relaxing - sometimes at the private beach, other times at the beautiful pools, and every morning from the balcony - and what a great view from the 9th floor! The unit has - what else?? - a beach theme that is so refreshing! The staff are readily available if need be, and no worries about parking as it is secure. We definitely will return and we have also recommended to other family members! A truly positive experience!!"Guest: Sheila Date of Stay: August 2015 Review Submitted: September 15, 2015View listing
"Good place to stay with family"Guest: aliaksei z. Date of Stay: April 2016 Review Submitted: May 10, 2016View listing
"We have stayed in this condo for 6 years. The people we rent from are great. The condo is perfect it's clean and right on the beach. Location is great Subway, pizza, liquor store, and go carts right across the street. We love that the tower is small and your not living on elevators for ever to get to the beach. It's quite and the beach area is not crowed. We will be renting again!"Guest: Repeat Renters Date of Stay: April 2016 Review Submitted: April 27, 2016View listing
"We loved our stay. Excellent condo that was very clean. Great heated pool and great beach. Excellent price. Loved it."Guest: Anthony A. Date of Stay: November 2015 Review Submitted: November 27, 2015View listing
Featured Destinations: Dauphin Island, Isle of Palms, Bald Head Island, Destin Condo Rentals


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Comments about 'High school boys basketball: Viewmont overcomes Highland's 3-point barrage, finds ways to win'
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I vote that ALL private schools regardless of enrollment start in the 3A classification. Because of their ability to attract students beyond the boundaries of the school district, it puts the 1A and 2A schools at a huge disadvantage. Private schools have resources that public schools don't.
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Season 1, Episode 1 Top Half of 1%
First Aired: June 23, 2009
An unscripted series that tracks a clique of teens in Manhattan's privileged prep-school scene. In the premiere, dramas twirl around seniors Jessie and PC, who share a romantic history; sophomore Sebastian casts his Lothario spell on Kelli; Camille's preoccupation with Harvard may be stifling her social life; and public-school student Taylor inches toward Sebastian.
Cast & Details See all »
- Premiered: June 3, 2009
- Rating: TV-14
- Premise: An unscripted series that tracks a clique of teens in the privileged prep-school scene in Manhattan as they study, party, shop, date and schmooze for connections. Among the players: a female sophomore hoping to score a rich hubby and a male sophomore with… (more)
Trending TonightSee all »
The Real Housewives of Orange County8:35 pm | BRAVOI'll Watch1414 Watching
The Real Housewives of New Jersey8:46 pm | BRAVOI'll Watch965 Watching
Dark Matter10:00 pm | SyfyI'll Watch174 Watching
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- 1. Rami Malek
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"Be loose!" The legendary multimedia artist, musician and film director Laurie Anderson puts it as simply and clearly as that when she here advises artists to avoid being pressured into…+ More details
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Test of color grading on an ungraded frame from Melancholia (2011) shot in Arri Alexa (Log-C). Using a LUT to give an interesting look to the image. Skin tones are pre-adjusted on second step via…+ More details
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Journey with us from the glamour of Paris to the sun-kissed hills of Hollywood and discover how Igor Stravinsky was able to transform his art throughout his extraordinary life. One the greatest icons…+ More details
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I went from a little Volvo convertible to a dodge ram 1500. I LOVE it! I can see everything on the road and people are scared of me instead of me being scared of them! What do you drive?
OT: for fun What car do you drive?
Posted 01/27/2014
There was no butler, but I did use coconut oil on my hemorrhoids, so...I'm pretty much royalty--Faithyday
Mollyd21803 · Original Poster
Posted 01/27/2014
Haha I can't either that's why everyone gets out of my way:)
There was no butler, but I did use coconut oil on my hemorrhoids, so...I'm pretty much royalty--Faithyday
Posted 01/27/2014
Chevy suburban!!! I love my big suv I could not go back to a car anytime soon.
Posted 01/27/2014
2001 Chevy Impala. It's my first car...bought in 08 right before I went to college. It's got AMAZING storage capacity so when I have to move again it will come in handy for everything except my furniture.
Posted 01/27/2014
Kia rio here too :)
FTM to Isaac. 10/31/13•born at 34 weeks❤🎃✨
Posted 01/27/2014
2009 Chevy Impala. I did have a 2007 Kia Rio but it had way too many miles on it and it was on its last leg so I got the impala.
Last edited 01/27/2014
2011 Ford Taurus SEL...I love it! We got it a month after LO was born. It is very sturdy and I feel we are safe running our errands.
Married to my hero 6/9/07.
Our lives forever changed 10/17/13 when we met our Clara Joy.
Posted 01/27/2014
I love it. 2009 and no issues or repairs needed, just regular maintenance!
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What's next for your growing child?
Get a glimpse of what's to come.
Read about 34 months : Week 1.


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For the first time, First Solar became the world's largest photovoltaic EPC contractor in 2012, with more than 500 MW of projects completed. » more
2013 40 Under 40 award winners
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1941 Wallace China Airbrushed Coffee Cup Surfer Girl Graphics Vhtf Wonderful
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1941 Wallace China Airbrushed Coffee Cup Surfer Girl Graphics Vhtf Wonderful:
Fresh to is thisWallace China Restaurant Ware coffee cup. I have collected restaurant ware for years and have given talks on the subject. This is a very hard to find pattern that shows an airbrushed surfer girl. There is glaze missing to the handle and rim of cup. Inside is bright and shiny with no utensil marks. No chips or repairs. Nice thick guage. This Wallace mark was used from late 30s to early 50s. Date code of 9-K puts the manufacturing of this item to Nov. 1941. If you are into this stuff, do not pass up this piece. I double you will find another one for a very long time.
Shipping will be parcel postmail. Please wait for invoice. Refunds given but buyer pays for return shipping. Contact and/or payment must be made within 3 days of sale closure ( rules!).After that time I am at liberty to relist without further explanation. I never charge a packaging or handling fee as I used recycled materials and feel shipping is already too high. Same day shipping if payment is made early enough in the day. Always 24 hours shipping except for days when post office is closed. Thanks for stopping by and viewing my listing. US buyer only please. Have fun offerding!!
1941 Wallace China Airbrushed Coffee Cup Surfer Girl Graphics Vhtf Wonderful:
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Set 4) Caribe Puerto Rico Pedestal Mugs White Cups Green Base Usa Diner 9oz 1971
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3 Restaurant Creamer Homer Laughlin New Port Gold Jackson Gladiator & Other Hl
Walker China Vitrified Bedford Ohio 7-33 Dogwood Flower Salad Plate 1950's Euc
Restaurant Ware Wallace China 4 X 7" Bread Plates Tan Los Angeles Calif 1950's


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List all words ending with fuci
Only the word fuci was found, nothing longer.
Some random words: sea phragmoplast psych vying feign briar lone
This is not a dictionary, it's a word game wordfinder.
- Help and FAQ - Examples - Home
Privacy and Cookies Policy - Share - © Copyright 2004-2016 Morewords.com - 61.126mS


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Deficit Fraud Jon Kyl: 'You Should Never Have To Offset' Tax Cuts
Sen. Jon Kyl (R-AZ) has been one of President Obama's most vocal critics on the budget deficit (most of which is actually attributable to the President's predecessor). "The Obama administration is spending trillions of dollars we do not have on things we do not need," Kyl has said.
But today on Fox News Sunday, Kyl threw his concerns about the deficit out the window when discussing tax cuts. Kyl said Congress should not allow the Bush tax cuts to expire, but when host Chris Wallace asked, "How are you going to pay the $678 billion to keep Bush tax cuts for the wealthy?" Kyl wouldn't answer. And in fact, he went so far as to say tax cuts should never have to be paid for:
WALLACE: We're running out of time, so how are you going to pay $678 billion just on the tax cuts for people making more than $250,000 a year?
KYL: You should never raise taxes in order to cut taxes. Surely congress has the authority and it would be right, if we decide we want to cut taxes to spur the economy, not to have to raise taxes in order to offset those costs. You do need to offset the cost of increased spending. And that's what republicans object to. But you should never have to offset cost of a deliberate decision to reduce tax rates on Americans.
Watch it:
Kyl is not only a deficit peacock, but he's also a deficit fraud. On the one hand, he attacks Obama for rising deficits but at the same time says that multibillion dollar tax cuts "never" have to be offset.
Earlier this year, Kyl defended Sen. Jim Bunning (R-KY) for blocking a measure to extend unemployment benefits. "All Senator Bunning was saying quite correctly is it ought to be paid for," Kyl said. So while Kyl advocates on behalf of the wealthy, he has no problem reverting back to being a deficit hawk at the expense of the less well-off.


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Yuka Oiwa is a summer intern through the Summer on the Cuyahoga program at the Ingalls Library in the Cleveland Museum of Art. Learn about her experiences working with the director of Library and Archives and other special projects.
The Thinker: A CMA Blog
#get2knowcaravaggio Contemporary Art textiles Photography Events international conservation performing arts behind the scenes interview Collection podcast Exhibition art senufo curator Video Caravaggio Parade the Circle treasures of heaven exhibitions playlists wari Gauguin Education this week at cma university circle The Cleveland Museum of Art visitors guest artists film five things happening this week Cleveland African Art museum birthday community arts Special Exhibition music Paul Gauguin: Paris


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Otto Ole Schnellbacher
Position: DB-E
6-4, 188lb (193cm, 85kg)
Born: April 15, 1923 in Sublette, KS
Died: March 10, 2008 in Topeka, KS
College: Kansas
Weighted Career AV (100-95-...): 0 (15060th overall since 1960)
Draft: Chicago Cardinals in the 25th round (231st overall) of the 1947 NFL Draft.
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instructional designer jobs near Richmond, VA
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Senior Instructional Designer - eLearning, Storyline, Camtasia
nextSource - a Workforce Solutions Company - Glen Allen, VA
Instructional Designer – Sales Cloud/Service Cloud Platforms. Applies recognized instructional design methodologies for adult learning....
Senior Instructional Designer - 100% Onsite
nextSource - Glen Allen, VA
ISD Level + Program and Curricula designer. Applies recognized instructional design methodologies for adult learning....
Epic Certified Principal Trainers - International Assignment
Virtelligence - United States
Strong experience working in a Principal Trainer / Instructional Designer capacity with. Virtelligence is seeking multiple Epic Certified Principal Trainers to...
Senior Graphics Designer (contract role)
Vertex Solutions Group, LLC - Virginia
This senior graphic designer will work with marketing, sales, instructional designers, project managers and web programmers to conceive and create appropriate...
Subject Matter Expert, Virtual - Apple iOS Cloud Integration
Rasmussen College - United States
Participating in regular review sessions with an instructional designer. Subject Matter Experts (SME's) will act as Contractors assigned to collaborate with an...
Epic Instructional Designer - CPOE
Innovative Consulting Group - United States
Our west coast client is looking for a instructional designer with MST experience, proficient or certified in Epic Inpatient/Ambulatory Orders (CPOE)....
Epic Optime Anesthesia Principal Trainer
Innovative Consulting Group - United States
Our East coast client has a need for a certified OpTime/Anesthesia Instructional Designer to assume responsibilities for the current EMR Principal Trainer for...
Curriculum Developer - Part Time
VA Retirement System - Richmond, VA
Ability to analyze, interpret and explain complex rules and regulations - Strong verbal and written communication skills - Experience with graphics and...
Instructional Designer and eLearning Developer
VA Housing Development Authority - Richmond, VA
The Instructional Designer & eLearning Developer will design, build, maintain, and refresh e-learning content for VHDA....
Instructional Designer
Calvary Counseling Center - Richmond, VA
Collaborates with Program Manager on instructional design issues, creates customized instructional elements, and manage course development....


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|Name: _________________________
|Period: ___________________
This test consists of 5 multiple choice questions, 5 short answer questions, and 10 short essay questions.
Multiple Choice Questions
1. Where did Daisy work?
(a) County General Hospital.
(b) Crew Street School.
(c) Nowhere; she never had a job.
(d) The Wainwright mansion.
2. Where has Daisy heard Martin Luther King preach?
(a) On the radio.
(b) At his church.
(c) In person.
(d) Over the TV.
3. How old is Boolie when the play ends?
(a) 75.
(b) 70.
(c) 65.
(d) 80.
4. To whom is Driving Miss Daisy dedicated?
(a) "Suzy."
(b) "Mama and Will."
(c) "My beloved wife."
(d) "To all the Miss Daisys out there."
5. What item is Boolie amazed Daisy didn't break in her car accident?
(a) Her cane.
(b) Her glass cat figurine.
(c) Her fingernails.
(d) Her glasses.
Short Answer Questions
1. What church does Martin Luther King preach at?
2. How does Boolie feel about his mother going to the banquet?
3. Where is one of Hoke's family members a professor?
4. What did Daisy demolish in her car accident?
5. Who does Daisy tell Boolie that Hoke came to see (in the final scene)?
Short Essay Questions
1. How long has Hoke been looking for work when he first meets Boolie?
2. What was Daisy's childhood like?
3. To what is Hoke referring to when he mentions the "Geechee trash" in Savannah?
4. How does Daisy react when Hoke tells her the temple has been bombed?
5. What happens to Daisy when Hoke exits the car during their ride back from Mobile?
6. What was Hoke's job during the war?
7. Why doesn't Hoke want to go to Savannah with the Stone family?
8. How does Daisy help Hoke find the Bauer headstone?
9. In the preface, what does Uhry have to say about a real Hoke?
10. What is the first "bad act" that Daisy accuses Florine of doing in the play?
This section contains 527 words
(approx. 2 pages at 300 words per page)


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Even though we ARE Nikon lovers,we are NOT affiliated with Nikon Corp. in any way.
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2700 posts
"RE: D1X for astrophotography??"
I've not tried astrophotography, but the general rule with digital cameras seems to be that you're better off using longer exposures at lower ISOs than shorter ones at higher ISOs. Longer exposures increase your chances of seeing individual "hot" pixels, but increasing the ISO amplifies the background noise so you get more "hot" pixels.You can see this type of effect in the DPReview D1h night exposure examples: 15 seconds at ISO 200 much is cleaner than 5 seconds at ISO 800. Interestingly, though, there seems to be a trade-off for very long exposures with 30 seconds at ISO 200 being about the same as 5 seconds at ISO 800.Keeping the camera cool (I mean letting it adjust to the ambient temperature, not putting it in the fridge ) seems to help reduce noise.Alan.P.S. Looking forward to seeing your photos of the planets... what sort of telescope were you using?


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NYE Bike Ride and Outdoor After-Party
Activist urban-cycling organization Time's Up! hosts this ride to Belvedere Castle in Central Park for a dance party with music by marching band Rude Mechanical Orchestra and fireworks at midnight.
Wed., Dec. 31, 10:30 p.m., 2008
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Crazy Substitute Teachers
Enjoy, NG4.01 / 5.00 2,113 Views
Vacuum Salesman
This dude and his Megazord vacuum cleaner4.00 / 5.00 2,615 Views
Markiplier Animated | Mark Of The Survey Corps
This took far too long to make...3.95 / 5.00 5,460 Views
-.- .....
WHAT IS THIS ? è_é HOLY SHIT! THIS IS A PIECE OF CRAP !!!
THERE IS NO HUMOR ... and that poor cat ç_ç
You know what's less funny that animal cruelty? People who do this: -.- It's gay and looks like Japanese cartoons or soemthing.
It had a Ren and Stimpyish kinda look to it, but dont watch it if you like cats.
Ren and Stimpy was a huge inspiration. I was trying to do the thing where their heads deform as they talk (like the scene where John gets mad), but sorta lost it (where John talked to the vets). Then of course the title screen was a blatant rip-off, but meh
It was not fun at all, im a vegetarian so you shouldn't have done THAT with the cat.
awww poor little pussy
meh...i didnt care for it dat much
nice animation
pretty good animation, but it's kinda weird with the 3-d buildings and the character animation. wut glitches would u go through??? no action script or anything
I had this problem where the audio would play before it was supposed to. It was extremely annoying 'cause I actally use lip-syncing as opposed to movie clips.


text:
List all words that contain absenteeisms
Only the word absenteeisms was found, nothing longer.
Some random words: epact niacin gnar befuddle fub aorist fub
This is not a dictionary, it's a word game wordfinder.
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text:
Nikon Coolpix S5100Pocket-lint
Nikon updated assembled journalists on the progress its made in strengthening the Coolpix brand, through its extensive "I AM" campaign. Many of you will probably have seen its sponsorship of Hollyoaks, as the company tries to capture a younger audience.
Fitting that profile is the new Nikon Coolpix S5100, which joins its line-up of "style" cameras.
Leaked and subsequently announced, Pocket-lint were on hand as Nikon showed off the camera and we grabbed some shots for your viewing pleasure.
The new Coolpix model comes with a 12-megapixel sensor and a 5x zoom lens, offering an impressive F/2.7 at the widest angle, which should help the low light performance.
Also boosting the low light performance is vibration reduction (VR, or image stabilisation as others call it) and an ISO range that will let you shoot at up to ISO 3200 for those really gloomy shots you just need to capture. The camera will also detect when it is attached to a tripod or being handheld and pick the appropriate settings for you.
Add to this the HD (720p) video capture and compact dimensions (97.1 x 56.9 x 21.6mm) and you have an attractive package for your pocket.
Unfortunately the price is still to be determined.
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Pocket-lint Adventures: Monkeying around on Go Ape with TomTom Bandit
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text:
Evan Rachel Wood Says She's Still Bisexual After Marrying Jamie Bell
Evan Rachel Wood confirms she still likes a girl. The Queen Sophie-Anne of "True Blood", who just got married to her actor boyfriend Jamie Bell, set the record straight on her sexuality when answering a fan's question on Twitter.
"Honestly not trying to be an a**. Just trying to understand. Does this mean u r not bi anymore? [How does] that work?" one of her Twitter followers asks the question, to which the newlywed replies, "No. It just means i am not single anymore."
Evan and Jamie first met on the set of Green Day's "Wake Me Up When September Ends" music video. They dated on and off before finally tying the knot in front of family members and close friends in a secret ceremony on Tuesday, October 30 in California.
The marriage news came to surface after they were spotted wearing their wedding bands. "Words cannot describe the happiness i am feeling. Overwhelming," she tweeted. "Thanks for all the congratulations! Many thanks to you wonderful people. :)."
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text:
Top ten signs you're an iPod junkie
To paraphrase an old saying, it's okay to love your iPod…just don't love your iPod. So in the name of helping you determine whether you're addicted to your iPod, here are the top ten signs that you're an iPod junkie: (Click 'Read more')
10. You write letters to your Congressman, urging the creation of a national "Take your iPod to work day".
9. You can name every single retail chain that sells the iPod, including stores that you'd never previously heard of and don't know where they're located.
8. You spend more time in the iLounge forums than the moderators.
7. For the first time in your life, you're tucking in your shirt…and it's only so that others can see your iPod on your belt clip.
6. You have six brands of iPod cases, including four that you've never used.
5. You've submitted an online review of the iPod's solitaire game.
4. You've purchased iTunes tracks from bands you don't even like, simply to boost your iPod's total track count.
3. You wear your earbuds even when your iPod is on its charger.
2. You have a battery backup for your battery backup…just in case.
and the number one sign that you're an iPod junkie…
1. You sold your car and moved back in with your parents just so you can afford give everyone on your list an iPod this Christmas.
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Marco Polo ft. Big Daddy Kane - Nite & Day
Posted 3 years ago
Tags: Marco Polo , Downloads , Indie , Lyrical
Marco Polo, cuts by Shylow
Fresh off garnering positive Booth reviews for What's Wrong, an album outtake co-starring rap legend Rakim, Marco Polo has pulled another unreleased gem from the vault for our listening pleasure. Like its predecessor, Nite & Day boasts guest bars from one of NYC's elder statesmen. This time around, it's Bed-Stuy veteran Big Daddy Kane who handles mic duties; over the Canadian beatsmith's airy samples and boom-bap percussion, the hip-hop icon deftly deconstructs the "baller image" that many of today's rappers maintain. Shylow assists behind the wheels of steel. Fans can look forward to more dope instrumentals and top-shelf guest features on Newport Authority 2, coming soon via Soulspazm/Rawkus Records.
Marco Polo DownloadsIndieLyrical
Written by richard
Posted 3 years ago
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More from Marco Polo
Artist: Marco Polo
Album: Newport Authority 2
With his latest full-length, PA2: The Director's Cut, slated for release this July via Soulspazm Records, veteran beatsmith Marco Polo is tiding fans over with the release of free album Newport Authority 2.Mixed and... More
Posted on May 01, 2013
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text:
Janet Long Overview
Janet Long has been associated with two companies, according to public records. The companies were formed over a ten month period with the most recent being incorporated nine years ago in April of 2007. One of the companies is still active while the remaining one is now listed as inactive.
Background + Contact Information Available for Janet L. Long
Contact + Background Information
- Phone, Address & Email
- Criminal & Civil Court Records
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Sponsored by PeopleSmartNo Thanks
Companies for Janet Long
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A connection is made when two people are officers, directors, or otherwise associated with the same company. Janet Long has only one known connection.
Michele C. Whiddon has the following companies in common with Janet Long
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Janet C Long
Clearwater, FL
Clearwater, FL
Treasurer at Regional Cooperative Alliance, Inc.
Vice President at Worknet Pinellas, Inc.
Janet C Long
Seminole, FL
Seminole, FL
Director at Garden Spot, Inc.
Director at Florida Public Affairs Services, Inc.
Janet W Long
Clearwater, FL
Clearwater, FL
Director at Hoffman's Appliances, Inc.
Florida Department of State
Data last refreshed on Thursday, April 21, 2016
Data last refreshed on Thursday, April 21, 2016


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Win prizes, kudos, recognition! Enter AOP prestigious Photography Awards before 7 August
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Is this attraction free to the public?
Would this be a good rainy day activity?
Thanks for helping!
Known for its busy streets and shopping
Great local street food, pedestrian streets and markets available within few hundred meters from the Mongkok MTR station. Only negative aspect, beware of pick-pockets since the...
read more
3 Days in Hong Kong
Discover Hong Kong's Living History by Tram
Hong Kong is not just for shopping, see the sites
Great local street food, pedestrian streets and markets available within few hundred meters from the Mongkok MTR station. Only negative aspect, beware of pick-pockets since the area is one (if not the) most crowded place on earth. Worth a walk for the experience/living.
I love Mongkok and stay at the Cordis that's part of the Langham Place shopping centre there. You walk out into the street and it's always busy. The street market is interesting. I used to pick up some gifts there, but the last couple of times I haven't bought anything there. Loads of shops for everything you need.
If you like little shops and markets, then this place is for you. Its extremely busy and full of bustle. There are plenty of little shops and markets to walk around.
This is said to be most populated part of the world when it comes to number of people per square km and as soon you get out from MTR you will soon realize it's true. There are plenty of shops too, stating from electronics, clothes, medicine to plenty of food options. Very busy at night too.
Unfortunately thousands upon thousands of mainlanders also visited with the hope of filling their suitcase with local products (not same quality in mainland China).....I do not enjoy these shoulder-shoulder crowds in the least. Did not see anything I felt tempted to buy....was rather craving to be inside an aircon shop, running from the stifling humidity and heat.
You want decent shopping spot?! Go to Mongkok. That is the answer I got from most locals I asked about best shopping spots in HK. From ladies market, where you can get good deals, to the main well known brands, like H&M etc. Don't forget to pop up at Bonjour; crazy discounts on perfumes and cosmetics. There are also few...
MONGKOK is a place where u can go on a shopping spree.. there is ladies market where u can shop for souvenirs ,bags nd all, there is sneakers street.. but in ladies market there are chances of getting cheated so bargain, bargain nd bargain..
Know your price first before buying. This place have too much bargain items! Everything you want to buy is already here!
Just my advice based thru experience, buy your stuff on stores located on the buildings. Some shops on the tents and kiosks will start you up on a high price and will start negotiating for your wanted price. Such...
Shopping in Hong Kong is always fun, especially for phones or cameras. Try out the camera just bought to capture the hustle and bustle could be real fun.
It is a dynamic area for shopping lovers. Also, compared to other places, massage is relatively cheaper in Mongkok area.
I will have a 14.5 hour layover in HK in a few weeks. I have booked a ticket for the hop on hop off bus. Which is the best stop to get on if I want to spend most of my time in the kowloon area, For late lunch I would like to be in Mody Street Area. Which would be the best way to go and get to Mongkok?
We are going to Hongkong this July for a short family vacation and planning to do some shopping for my girls ( ages 2 and 6 y/o) too. Can you please suggest places/ shops where I can go for shopping ( with reasonable prices). I am specifically looking for some trendy dresses.
Where is it located at? How can I travel to there from prince edward station?
Hi, please tell me that would it be cheaper to take a bus or train from Geneva to Chillon?
Hi! I am travelling to HK soon and I am looking for mahjong shop to hand carve the mahjong set.
As I am staying at Mongkok .. is there any shop nearby?
Otherwise can recommend any other shops where are good and convenient to locate?
See all questions


text: On Wednesday 30 August 2006 22:59, reinhard amersberger wrote: > hi, > > I also cannot compile kdenlive on my machine at home (suse 10.0): > > TrackPanelMarkerFunction::mouseReleased(Gui::KTrackPanel*, QMouseEvent*)': > /prog/svn/kdenlive/kdenlive/trackpanelmarkerfunction.cpp:72: error: > 'kdDebug' was not declared in this scope Should be fixed now, try updating. Let me know if any of you have further compile problems. jb


text:
Nagravision has launched its end-to-end push video-on-demand security solution for digital video broadcasting networks over the Premiere network in Germany.
The Nagra DVR Push Content-on-Demand secures Premiere's new VOD service PREMIERE DIRECT+, the Premiere home video rental store, which enables subscribers to get up to 30 movies per week downloaded on to the Premiere Digital Recorder (PDR). The movies are downloaded onto the hard-drive via a push video-on-demand system that operates via satellite. After the order via phone, text message or Internet, the movies are available for the viewer during 24 hours. Movie watching can be paused, rewound, fast-forwarded, viewed in slow motion and seamlessly resumed at will.
Nagra DVR Push Content-on-Demand is a complete end-to-end solution for operators wishing to provide transaction-based content-on-demand on digital video recorders.
For more information, visit www.nagra.com.
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...I graduated from Brandeis University with a double major in Spanish and history. I'm a certified high school Spanish teacher, and am currently pursuing an MA in Spanish literature at Loyola University. I've studied abroad in Cuernavaca, Mexico and Madrid.
Evanston, ILView Tutor
...I tutor K-6 math, Pre-Algebra, Algebra 1, Algebra 2, Geometry, Trigonometry, and Pre-Calculus. My style uses solid Math teaching and Communication Skills coupled with patience, encouragement and positive reinforcements. I am married and a father of a boy and a girl and enjoy teaching my kids math.
Morton Grove, ILView Tutor
...You first have to figure out what the question is looking for then go step by step making sure you write everything down and then coming to the correct answer. I would work at the student's pace and make sure they understand the basics since you do a large amount of work with equations which are...
Bolingbrook, ILView Tutor
...If you have practice identifying these errors, you can outsmart the SAT on this section! With my resources and past experiences, I guarantee a significant increase in your scores. Having received tutoring myself for an SAT II Subject Test, I can personally attest to the potential gains to be made by working with a tutor, since my score improved.
Chicago, ILView Tutor
My name is Marty, and I would love to tutor your children in whatever they need help in. I majored in History at the University of Illinois at Urbana-Champaign, and also achieved an Associates in Science from Parkland Community College. For my Associates degree, I studied mostly chemistry and math, but also physics and biology.
Tinley Park, ILView Tutor


text:
UEN News & Notables
Now Showing on UEN-TV
Eating Alaska
EATING ALASKA is a serious and humorous film about connecting to where you live and eating locally. It is about trying to break away from the...
Now Showing on MHz Worldview
While investigating the case of a murdered junkie, Caïn takes it upon himself to launch a different investigation. Directed by Bertrand Arthuys,...
Now Showing on FNX
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Six urban Natives are taken from the city and deposited in the bush; the start of their week living as their ancestors did.


text:
driver jobs near Saint Petersburg, FL
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CT Apollo Regional-Seeking Class A CDL Drivers for Hire
CT Transportation LLC - Saint Petersburg, FL
What could you do with an EXTRA $400(or more)?If you have a free weekend here and there and want to earn more money, become one of our Weekend Warriors!...
Automotive Express Lube Technicians, Exp. or Trade School Required, Busy, State-of-the-Art Shop
Suncoast Chrysler Jeep Dodge Ram - Seminole, FL
Minimum of 1 year of experience as a Lube Technician (automotive mechanic) OR recent completion of automotive tech/trade school Automotive Service Excellence ...
Come Drive for CCC Transportation moving PODS OTR
CCC Transportation - Tampa, FL
Drivers will be away from home at least 4 weeks at a time with *incentive pay for staying out longer.*. Or Call A CCC Transportation Recruiter at 888-690-3011*....
Delivery Driver
Performance Food Group - Tampa, FL
Great food and great careers – this is what Performance Food Group brings to the table. High School Diploma or Equivalent. Class "A" Divers License....
South East Regional Truck Driver - CDL Class A
CYPRESS TRUCK LINES/SUNBELT - Florida
We have rewarding opportunities for experienced drivers, recent graduates and students looking to get their Class A CDL. $1000 Driver Referral Bonuses....
TRUCK DRIVER - CDL Driver LOCAL (Transportation)
TransForce, Inc. - Tampa, FL
APPLY NOW OR CALL 813-514-0883 FOR MORE INFORMATION. Truck Driver - CDL Driver (Transportation). 1 Year recent tractor trailer driving experience....
New Truck Driver Job - No Experience Necessary & Great Benefits!
US Xpress - Clearwater, FL
Please call for current offers and information. Don't Wait, Call Now! New Truck Driver Job - No Experience Necessary & Great Benefits!...
Local CDL-A Company Truck Driver
Florida Rock and Tank Lines - Saint Pete Beach, FL
And since we pay you to learn the ropes, you don't have to worry if you've never pulled a tanker before! Weekend pay incentive & night differential....
TRUCK DRIVER - CDL A Local Driver (Transportation)
TransForce, Inc. - Tampa, FL
APPLY NOW or CALL 813-514-0883 FOR MORE INFORMATION. Truck Driver - CDL Driver (Transportation). Dedicated Runs - Great Pay - Good Quality Equipment....
Owner Operators Needed To Run OH-TX-FL Triangle (6905238)
General Transport - Saint Petersburg, FL
Our Owner Operators will run that triangle Weekly miles range from 2,500-3,200 Weekly Pay through Direct Deposit Well-maintained trailers with no trailer rent...


text:
Crazy Substitute Teachers
Enjoy, NG4.02 / 5.00 2,060 Views
Vacuum Salesman
This dude and his Megazord vacuum cleaner4.00 / 5.00 2,602 Views
Markiplier Animated | Mark Of The Survey Corps
This took far too long to make...3.94 / 5.00 5,298 Views
It rules
yeah man. â-² RULES â-²
I Absolutely love the part where strawberry jumps out of the ten story building just to save his buddy asshat clock. All my fives


text:
Question: Could watching Porn be harmful from a Buddhist point of view?
I know that personally the less distractions I have in daily life the more I can stay focused, grounded and be mindful on what's going on in and around me. Too much TV, internet, drugs and porn can create a confused and stressed mind. So if one needs to masturbate it's better without looking at porn of any kind, just be mindful on what's going on in the here and now, the sensations etc. What do you think?
My answer:
From a "Hinayana" point of view, less is definitely more. It's good to focus on building a foundation of sanity and mindfulness. But there's certainly no moralistic problem with porn, beyond the fact that it could (or not) wrap your mind up in lust, not getting out and meeting actual men or women…that said, masturbation is healthy, of course, no problem there morally either.
That said, some porn is degraded, and that's probably the equivalent of eating factory farmed food vs. wholesome food that came from a local farm. Some porn is more respectful and fun and uplifted, and that might be more healthy, mentally, to spend time enjoying.
Just my two cents.
Going beyond that, from a "Vajrayana" point of view, as Trungpa Rinpoche said, "Chaos should be regarded as extremely good news." IE lust, porn, distractions generally (you see that too with the "fly" in Zen Mind, Beginner's Mind being a symbol of how distraction can remind us to wake up, and actually be a vehicle to speed our path if regarded as such) are not a problem, and are in fact fun—distractions are welcome, transmuted, and celebrated. Still, that can be tough work…lust gets many of us and encouraging lust can be unhealthy…and philosophizing on transmuting lust can be easier than actually self-liberating it (which can be…hard).
Either way, practice makes perfect!
For the full discussion and other points of view, click over to r/Buddhism
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Live Travel incidents in Barrow-in-Furness
For details and planned incidents see BBC Travel
Weather Beta
Search for locations to get quick access to weather information in the places you care about.
Sunrise 05:22
Sunset 21:16
Low 14°C 57°F
Clear Sky
Any showers will ease this evening to leave a dry and quiet night. Clear spells will develop and it will feel cooler than recent nights.


text:
Thanks for all the feedback, it was pretty much obvious the bottom logo was the one! Now we're moving forward with it. :)
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