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Farr 40 World Championship - Defining juncture of the season
by KPMS on 24 Jul 2013
Tasmania's Voodoo Chile mixes it with the best in the Farr 40 worlds. © Andrea Francolini Photography
The Rolex Farr 40 World Championship is the defining juncture of the Farr 40 Class international racing season. It proposes a fascinating forum for the evenly matched 40-foot racing boats, helmed by skilled and competitive amateur owners and guided by world-renowned tacticians.
Newport, Rhode Island, is the venue for the 16th edition of the championship, which takes place from 27-30 August and will be hosted by the New York Yacht Club. It is the third time that the epicentre of sailing in the United States has welcomed the event that has captivated the sailing community since its inception in 1998.
Fiercely contested, the Rolex Farr 40 World Championship guarantees close quarter sailing, while demanding and rewarding teamwork, consistency and determination. Together with the true sporting conduct of the competition and the participating teams' quest for perfection, these core values align with those of Rolex, title sponsor of the competition since 2001.
The 2013 edition promises to live up to the regatta's elevated standards. Nearly twenty international crews are expected to sail in a contest where the margins between success and failure are precarious. Last year's event which was hosted by Chicago Yacht Club on Lake Michigan was claimed by a local boat, Helmut Jahn's Flash Gordon 6. Seeking to become the first crew to retain the Rolex Farr 40 World Championship title since 2008, Flash Gordon 6 faces tough competition – most notably from the crews that pushed her closest in 2012 – Alberto Rossi's Enfant Terrible from Italy and 2011 champion Transfusion, owned by Guido-Belgiorno Nettis of Australia.
The competing yachts are virtually identical, required to comply with strict one-design rules, ensuring that the race is focused on the team that sails best. The array of top-class professional sailors in attendance – up to four are permitted per crew – comprises the pinnacle of the sport. The professional tactician is a core component who must work in harmony with the crew's skilled amateur owner/helmsman.
The competition is organized by the Farr 40 Class Association. Racing commences on Tuesday, 27 August, with up to 10 short and fast windward/leeward races contested over four days. The triumphant crew is typically the one that demonstrates the finest display of teamwork with complex manoeuvres executed with split-second
Farr 40 Worlds website
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Another Republican Bites the Dust!!
Posted in the Millbrook Forum
1 - 20 of 909 Comments Last updated May 7, 2016
#1 Dec 5, 2011
Another one Bites the Dust,Another one Bites the Dust and Another one Bites the Dust!!
Another Republican Bites the Dust!!
#2 Dec 5, 2011
Dear Friend,
December 03, 2011 --- And when I say friend, I mean it in the normal way, not someone Ive been sleeping with for 13 years. Unless, of course, I have been sleeping with you for 13 years. In that case, I do mean it that way.
It is with a heavy heart that I have decided to end my inspirational quest for the White House. After much reassessing and reconsidering, I have decided to spend more time being screamed at by my wife. And by more time, I mean 24 hours a day, stopping only for bathroom breaks.
But before I go, let me share with you my final thoughts on my campaign. After months of crisscrossing this great land of ours and participating in over three hundred televised debates, I am being disqualified because of an extramarital affair.
I mean, lets get real. I never heard of Libya. I didnt know whether that CNN dudes name was Wolf or Blitz. And my only training for running the #1 nation in the world was running its #8 pizza chain. Yet none of that, I repeat, none of that disqualified me. In fact, I was the front-fucking-runner, as long as I kept my 9-9-9 in my pants.(I have no idea what I meant by that -- I just like saying 9-9-9.)
#3 Dec 5, 2011
You don't like Cain beacuse Cain is 100% Black as opposed to Obama who is only 50% Balc,... you are a racist!
#4 Dec 6, 2011
Dear wife who is dying of cancer:
I need a divorce right away.
#5 Dec 6, 2011
Newton Leroy Gingrich is a horse's a-ss, conniver, fraud, and a phony.
Trust him as far as you can throw your refrigerator.
#6 Dec 21, 2011
Another one Bites the Dust,Another one Bites the Dust and Another one Bites the Dust AND ANOTHE ONE BITES THE DUST!!
Another Republican Bites the Dust!!
Trump's debate
Newt NUMBERS!
#7 Dec 21, 2011
hey dyckbreath,... stick to what you know,,, skin flutes, homosexual laws, gay weddings, receptive anal intercourse,,,
#8 Dec 21, 2011
That's not how it happened, and BTW she's is STILL ALIVE today!
#9 Dec 22, 2011
I'm as dumb as a bag of hammers.
Why else would I use polyurethane to hold my hair down ?
Why else would I be seen with a fat ass like Newt Gingrich ?
#10 Dec 22, 2011
#11 Dec 22, 2011
Who will be the next Republican to bite the dust?
#12 Dec 22, 2011
The aforementioned conniver, Gingrich and his fat ass.
#15 Jan 9, 2012
Another one Bites the Dust,Another one Bites the Dust and Another one Bites the Dust AND ANOTHER Republican BITES THE DUST!!
Another Republican Bites the Dust!!
Michele "go to hell" Bachman Bites The dust!!
See ya!!!!
#16 Jan 9, 2012
stick to what you know,,, skin flutes, homosexual laws, gay weddings, receptive anal intercourse,,,
I am going to laugh my ass off when Obama is beaten in a landslide election as you and your moron crowd scream racism and other nonsense
#18 Jan 13, 2012
Well, 58% of Republican voters say they are not satisfied with the field of GOP President wannabes and want someone, anyone but the current GOP Field of Screams.
Will it be;
Rick (Can't debate anything...oops) Perry?
Newt (Serial Adulterer) Gingrich, who's know ticked off because he's the subject of attack ads?
Rick (I am the one) Santorum?
Ron (I can't seem to recall newsletters) Paul?
Jon (Who?) Huntsman?
Willard (Mitt; I like to fire people) Romney?
Herman (999; more like 666) Cain is gone.
Michelle (God told me to run for president of USA) Bachmann has finally come to her senses (if that's what is in her case) to bow out since she dosen't know how to be a politician, although she's been in Congress for 10 years.
The GOP Clown Show continues.
#19 Jan 13, 2012
Good to see you!
It is now Officially THRILLA TIME!!!
#20 Jan 13, 2012
Understood, much appr4ciated.
I see where the usual trolls are doing their continuance of ignorance.
I haven't visited this website in months and I still have an influence on the mental case poster with the multiple personalities.
Some things just never change with some folk.
Happy New Year!!!!!!!!!!
#21 Jan 15, 2012
ILAL and ZCS tarding love notes on line,... how sweet!
#22 Jan 18, 2012
The Manchester, NH Nutjob aka Goofus Doofus chimping in with another bizzaro world rant...., how pathetic.
#23 Jan 18, 2012
Add Jon Huntsman to the list.
Who's next.......
Willard (Mexican Mitt)?
What a sad sack of presidential wannabes; Obama is a shoo in to win relection, they're handing it to him on a silver platter.
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Sonya Goff
Published: Wednesday, December 3, 2003 at 10:54 a.m.
Last Modified: Wednesday, December 3, 2003 at 12:00 a.m.
THOMASVILLE | Sonya Carol Lambe Goff, 38, of Madisonville, Tenn., died Sunday, Nov. 30, 2003, at her home.
The funeral will be Thursday at 2 p.m. at Fair Grove United Methodist Church, Thomasville, by the Rev. David White and Dr. Carlos Peterson. Burial will follow in the church cemetery.
Mrs. Goff was born June 15, 1965, in Thomasville to Seibert C. Lambe and Sarah Black Lambe. She graduated from East Davidson Senior High School in 1983 and from the DeVry Institute in Atlanta, Ga., with a degree in computer science. She was a former employee of CSX Transportation in Jacksonville, Fla., and was a homemaker. She was a member of Christ Community Church in Athens, Tenn., where she served as the church library administrator. She home schooled her children and was a member of MOPS ( Mothers of Preschool Children). She was preceded in death by a brother, Scott D. Lambe.
Surviving are her husband, Kenneth Paul Goff Jr., whom she married Oct. 13, 1990; three daughters, Hanna Carol Goff, Emily Grace Goff and Evalyn Jeanne Goff, all of the home; a son, Kenneth Paul Goff III of the home; her parents of Thomasville; and her mother and father-in-law, of Van Cleave, Miss.
The family will receive friends tonight from 6:30 to 8:30 at J.C. Green & Sons Funeral Home, Thomasville, and other times at the home of her parents, 228 Fleer Road.
Memorials may be made to Christ Community Church Building Fund, 205 Washington Avenue East, Athens, Tenn. 37303 or to Fair Grove United Methodist Church Building Fund, 204 Cedar Lodge Road, Thomasville, N.C. 27360.
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Hotels in Monferran-Saves, France
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- Most bookedHostellerie du Lac
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"Four of us on a cycling trip. No problem storing the bikes. The place itself is magnificent, stately home style. Huge rooms, lovely views, good facilities."
A Traveller, Aug 2015, GB
Hotels within 10 miles of Monferran-Saves
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"Stayed at this nice, modern hotel for 3 nights - our second stay here as we have family nearby - rooms are comfortable and the staff charming. Market on Saturdays just outside on the square, however beware if you're a light sleeper and room facing front of hotel as the stalls setup from about 5am..."
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"We have stayed here now a couple of times on our way between our six cottages in France. The owners are welcoming and we look forward to the evening meal, which is always tasty and good value, as well as offering a good range of choices. This time the evening was a bit chilly, so the roaring log ..."
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"Excellent stay in this charming and friendly hotel."
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You'll also want to determine which type of accommodations will suit your needs before booking a hotel in Monferran-Saves. If you're traveling with the kiddos, a family friendly hotel will offer larger rooms and more sleeping arrangements. For those traveling for an extended period of time, booking a hotel suite with a kitchenette is a smart move and will feel a little more like home. If you are budget conscience and just need a place to rest your head, a hostel or motel may be all you need.
If you are looking to score the best discounts and deals, use the filter to sort by price from low to high. You can book a hotel in Monferran-Saves for as low as &PricesFrom per night. If you are looking to cut down on expenses, look for hotels that include free breakfast and parking. Some hotels even offer free shuttle service to and from nearby places. You can also sort by star rating, proximity, and guest rating.
Before you book, check out user reviews. Hotels.com has 0 reviews in Monferran-Saves. We show reviews from Expedia and TripAdvisor so you know that you are reading genuine, reliable reviews. Be sure to download the Hotels.com app so you can book your reservations on the go, view amenities, and save the hotels you want to view and compare later.


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While most Minnesotans understand that seat belts and child restraints save lives, thousands of drivers were cited not buckling up during the Click It or Ticket extra enforcement campaign from May 23 – June 5. Law enforcement handed out 7,233 seat belt and 213 child restraint citations to those who risk losing their own lives or others in the car. Among the violations, a 4-year-old riding on his mother's lap and helping steer the vehicle. More information is available in our recent news release.
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FBI investigating NYC woman's death in Istanbul
U.S. investigators were invited by Turkish authorities to assist as they try to find out what happened to Sarai Sierra
Associated Press
NEW YORK — A U.S. congressman says the FBI is playing a significant role in the investigation into the death of a New York City woman found dead in Istanbul while on a solo vacation.
Rep. Michael Grimm, a former FBI agent, says U.S. investigators were invited by Turkish authorities to assist as they try to find out what happened to Sarai Sierra. The 33-year-old mother of two went missing Jan. 21. Her body was found 12 days later.
Grimm and Sierra's parents, Betzaida and Dennis Jimenez, say the family's immediate concern is returning Sierra's body to the U.S.
Her husband, Steven, is in Turkey. He intends to accompany her body, but the family is still determining how to fund the transport.
Grimm says the family's priority is bringing her home.
Copyright 2013 Associated Press
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The Ashtabula, OH Job Market
Looking for a good job in Ashtabula, OH? Ashtabula, OH contains a population of about 18,700, based on 2013 government data. The median family gross income value of the city of Ashtabula, OH is approximately $29,000, based mostly on government statistics as of roughly 2013 Ashtabula's cost of living, as recent as 2013, was almost 80 out of 100, and is lower than the national average.
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"Justice League": Exploring How Superman Returns (Again)
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Pablo was Norman Granz's baby, and while fans of Ella, Oscar, Basie, Zoot Sims, Milt Jackson, and Sarah Vaughan remain grateful for the wide variety of settings he produced them in, it was Pass who enjoyed a first and lasting brush with fame through Granz's efforts.
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Temple Sinai
10 - 19
2.5 - 5 Million
Services->Personal Services->Schools
SIC Code:
8661 - Religious Organizations
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Additional Info:
This member of the Schools industry is located in Sharon, MA and you can find important contact information regarding significant company contacts on their Lead411 profile like linkedin, phone numbers, facebook and cfo details. The profile also contains Temple Sinai email addresses which have a domain of @temple-sinai.com format. If you sign up for our free trial you will see our email@example.com addresses.
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text:
|Publication number
|Publication type
|Application number
|Publication date
|May 6, 2003
|Filing date
|May 24, 2000
|Priority date
|Oct 23, 1998
|Fee status
|Publication number
|Mary S. Riley, Richard Provonchee
|Original Assignee
|Cbm Intellectual Properties, Inc.
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (9), Referenced by (2), Classifications (9), Legal Events (4)
|External Links: USPTO, USPTO Assignment, Espacenet
This application is a continuation-in-part of patent application Ser. No. 09/177,469, entitled "Vacuum Package for Electrophoresis Gel", filed Oct. 23, 1998, now U.S. Pat. No. 6,090,255 which is incorporated herein by reference in its entirety.
The present invention relates to packaging for electrophoresis gels and, more particularly, to an improved vacuum sealed package combination for containing and protecting electrophoresis gels during storage, handling and shipment.
Most biological macromolecules are electrically charged such that, when subjected to an electrical field, they begin to move, This phenomenon is the basis behind an electrophoresis process wherein a prescribed electrical current is generated adjacent to a collection of macromolecules to produce movement of the macromolecules through a solvent in a particular direction. Since different macromolecules vary in molecular size, weight and charge, it is possible to use an electrophoresis process to distinguish between different macromolecules based on their respective rates of movement through the solvent. Electrophoresis can also be used for other types of macromolecule analysis, such as detecting amino acid changes, nucleic acid sequencing and protein analysis.
One medium that has been used for over thirty years as the carrier for the macromolecules in an electrophoresis process is a gel. Two of the most popular types of gels are agarose and polyacrylamide gels. An electrophoresis gel is typically planar in shape and includes a series of spaced apart wells. The wells are designed to receive the biological sample being tested. In the past, it was customary for the laboratory that was conducting the testing to cast their own gels by hand. It soon became apparent, however, particularly as electrophoresis testing of DNA increased, that it is more convenient and more precise to use precast gel slabs made to uniform composition, size and well configuration standards.
Conventional gels are made from various materials depending on their intended use. One widely used gel material for DNA electrophoresis is agarose. This gel is a sugar based gel that is highly flimsy and subject to tearing and deformation if not handled carefully. Tearing or cracking is especially prone between the wells where the least amount of material is present. Deformation or tearing of the gel can potentially produce inaccuracies during testing. To complicate matters further, the material properties of the gel make detecting hairline cracks very difficult. Hence, proper packaging of the gels is extremely important when shipping or storing the gels.
Several packaging arrangements have been developed in recent years to protect electrophoresis gels during shipment. U.S. Pat. No. 5,443,704 discloses one packaging arrangement for protecting the gels. This packaging arrangement includes a plastic tub within which the gel is placed. A foil-lined cover is adhered to the top of the tub to retain moisture inside the package. It was subsequently determined that the tub alone did not provide sufficient protection for the gel. Filler materials had to be added to the box to provide adequate protection from damage. As a result, this type of packaging arrangement is generally very expensive to produce.
Another prior packaging arrangement for shipping gels includes a pouch formed from foil material that is sealed along each edge. In order to protect the gel, a separate sheet of material is folded over the gel to separate it from the foil. A deficiency with this packaging arrangement is that the package does not adequately protect the gel from damage such as edge deformation and well breakage.
U.S. Pat. No. 5,837,288 discloses one particular type of pouch wherein the pouch is almost completely evacuated of air prior to sealing, resulting in a reduced atmospheric environment (i.e., less than 10% of atmospheric air pressure). Spacers are used on the sides of the gel to prevent the package walls from crushing the sides of the gels.
Co-pending patent application Ser. No. 09/177,469, entitled "Vacuum Package for Electrophoresis Gel", filed Oct. 23, 1998, discloses an alternate package configuration which, in one embodiment, uses a support sheet in combination with a sealed, at least partially evacuated interior to restrain the gel within the package for preventing gel damage during storage and shipping.
A need exists for an improved packaging arrangement which is inexpensive to manufacture and which adequately protects a gel or series of gels from damage during shipment, handling or while stored.
An object of the invention is to provide a package for an electrophoresis gel which includes sufficient rigidity to prevent damage to the gel and minimize movement of the gel within the package. The wells in the gel make it particularly susceptible to damage and breakage during shipment.
Another object of the invention is to provide a package for an electrophoresis gel which, in one embodiment, includes a support tray that functions as part of the packaging during shipment and can be subsequently used to hold the gel during an electrophoresis process.
These and other objects and advantages of the invention are provided by the packaging arrangement according to the present invention. The packaging arrangement includes first and second sheets that are sealed along their respective edges to form an enclosed cavity. In one embodiment, the cavity is at least partially evacuated of air prior to or after sealing. At least one electrophoresis gel is disposed within the evacuated cavity. The partial evacuation of air from the cavity causes the top and bottom sheets to conform to the gel, thereby restraining it from movement within the package.
In one embodiment of the invention, a tray is incorporated into the package. The gel is located within the tray which, in turn, is located between the top and bottom sheets. The tray includes a base and at least two side walls which extend upward along the longitudinal sides of the gel. The combination of the low pressure environment in the package and the tray produces a rigid packaging configuration that minimizes motion of the electrophoresis gel contained therein.
The foregoing and other features and advantages of the present invention will become more apparent in light of the following detailed description of the preferred embodiments thereof, as illustrated in the accompanying figures.
For the purpose of illustrating the invention, the drawings show a form of the invention which is presently preferred. However, it should be understood that this invention is not limited to the precise arrangements and instrumentalities shown in the drawings.
FIG. 1 is a plan view of one embodiment of an electrophoresis gel package according to the present invention.
FIG. 2 is a partially broken away isometric view of the electrophoresis gel package, illustrating an electrophoresis gel contained within a partially evacuated cavity.
FIG. 3 is a partially broken away isometric view of another embodiment the electrophoresis gel package according to the present invention, illustrating a plurality of electrophoresis gels contained within a cavity.
FIG. 4 is a cross-sectional view of the electrophoresis gel package of FIG. 3 illustrating the interleaving of spacer sheets between adjacent gels.
FIG. 5 is a partially broken away isometric view of an alternate embodiment of the electrophoresis gel package, illustrating an electrophoresis gel contained within a tray in a partially evacuated cavity.
FIG. 6A is a cross-sectional view of the electrophoresis gel package of FIG. 5.
FIG. 6B is a cross-sectional view of the electrophoresis gel package of FIG. 5 that includes a tray with short side walls.
FIG. 7A is a cross-sectional view of one embodiment of the package with multiple gels interleaved by spacers.
FIG. 7B is a cross-sectional view of another embodiment of the package with multiple gels, each gel contained within an associated tray.
While the invention will be described in connection with one or more preferred embodiments, it will be understood that it is not intended to limit the invention to those embodiments. On the contrary, it is intended that the invention cover all alternatives, modifications and equivalents as may be included within its spirit and scope as defined by the appended claims.
Referring now to the drawings, wherein like reference numerals illustrate corresponding or similar elements throughout the several views, FIGS. 1 and 2 illustrate one embodiment of an electrophoresis package 10 according to the present invention. The package 10 is formed from two overlapping sheets 12, 14 that are sealed 16 along their edges to form an enclosed cavity 18. The cavity 18 is designed to contain at least one precast electrophoresis gel 20. One preferred gel 20 for use in the present invention is an agarose gel, such as 1% SeaKem� LE, sold by BioWhittaker Corporation, Rockland, Me.
In order to prevent the gel from moving during shipment, the package 10 is vacuum sealed 16 along its edges so that the sheets 12, 14 substantially conform to the shape of the gel 20. That is, the cavity 18 within the package 10 is preferably at least partially evacuated of air. Removal of air from within the package 10 stiffens the sheets 12, 14, thereby increasing the package's strength to better support the gel.
Most conventional sealed packages are not evacuated prior to sealing. As such, the residual air contained within the package results in the upper and lower sheets of the package essentially functioning independent from one another, i.e., they do no support one another. The independent movement of the sheets reduces the package's stiffness and allowing it to be easily flexed. The relative ease with which non-evacuated pouches can be flexed increases the chance that the malleable gel contained within the package will become damaged.
Additionally, the air trapped within a conventional package produces an interior volume which is considerably larger than the gel. Accordingly, the gel is generally free to move within the package. Gels are inherently slippery and some gels actually exude moisture, making them even more prone to sliding within the package. Since the gel is very soft and pliable, movement during shipment or storage increases the likelihood of damage to the gel.
The evacuated package design according to the present invention reduces damage to the gel 20 by stiffening the packaging and reducing the mobility of the gel. The vacuum packing/sealing of the packaging eliminates at least a partial amount of the residual air contained within the cavity 18 of the package 10 after formation. Preferably between about 1% to about 89% of the air within the package 20 is removed prior to sealing which causes the sheets to conform to the shape of the gel without crushing the gel. As a result, the upper and lower sheets 12, 14 overlap one another adjacent to the edges of the gel 20 and, thus, effectively function as a substantially unitary structure.
Furthermore, the drawing of the upper and lower sheets 12, 14 of the package 10 against or in close proximity to the top surface of the gel 20 prevents the gel 20 from moving within the package 10 by reducing the interior volume of the cavity 18 after the gel 20 is inserted into the package 10.
The sheets 12, 14 of the package 10 are each made from at least one and, more preferably, several constituent layers. In one embodiment, the layers form a laminate having a thickness of less than approximately 3.0 mil. More preferably, the thickness is about 2.5 mil. In one exemplary embodiment, each sheet 12, 14 includes a metallized outer layer, an intermediate layer and an inner layer. The layers are preferably adhered to one another with a suitable adhesive. Although the following discussion refers to the upper and lower sheets as being similarly configured, they need not be so. For example, the lower sheet 14 can be selected with a different thickness and/or material than the upper sheet 12. Those skilled in the art would readily be capable of making such modifications in light of the teachings provided herein.
One problem associated with agarose type gels, such as 1% SeaKem LE, is that they can deteriorate from loss of moisture when exposed to certain environmental conditions. Hence, it is desirable to protect the gel 20 from moisture loss by forming the package 10 from material that provides a suitable moisture barrier. In one embodiment of the invention, the package 10 is formed from sheet materials with a moisture vapor transmission rate (MVTR) of less than approximately 0.02 grams in 100 square inches over a 24 hour period. This preferred moisture vapor transmission rate allows the gel to remain viable for a 12 month shelf life. The moisture transmission rate is determined by measuring the amount of vapor loss through a 100 in2 piece of the packaging material over a 24 hour period of time while exposed to a temperature of 100� F. This test for determining moisture vapor transmission rate is disclosed in ASTM Standard F1249. More preferably, the moisture transmission rate is about 0.01.
In one exemplary embodiment of the invention, the outer layer is made from metallized polyester or polypropylene material with a thickness of approximately 0.48 mil. The metallized polyester protects the enclosed gel 20 from environmental factors. The outer layer preferably has a smooth surface appearance that accepts customary printing. Although straight foils can be used as the outer layer in the present invention, they are not preferred due to their typical crinkled and pock marked appearance which leads to poor print quality.
The intermediate layer is preferably made from polyester material with a thickness of approximately 0.48 mil. The inner layer is preferably made from low density polyethylene material with a thickness of approximately 2 mil. It is preferable to use low density polyethylene material since it is chemically compatible with an agarose gel. The film in this exemplary embodiment has a moisture transmission rate of about 0.01 grams at 100� F./100 square inches/24 hours.
The upper and lower sheets 12, 14 are sized to provide a cavity 18 within the package 10 that allows the gel 20. As discussed above, a seal 16 is formed along the edges around the package 10 to prevent air leakage. Of course, if the upper and lower sheets 12, 14 are contiguous along one side, i.e., folded over the gel 20, only three sides need to be sealed since the fourth side is inherently sealed. For a 6�10 cm gel, a single gel package 10 according to the present invention, is formed with upper and lower sheets 12, 14 that have a preferred width of approximately 9.91 cm (3.9 inches) and a preferred length of approximately 13.91 cm (5.48 inches). This permits a seal to be formed around the package having a width of preferably between about 0.635 cm (0.25 inches) and about 0.9525 cm (0.375 inches). This will leave an inside spacing of about 1 cm�2 mm all around the edges of the 6�10 cm gel 20.
To further protect the gel 20 from inadvertent bending, it may be desirable to incorporate of one or more support sheets 24. In one embodiment of the invention, a support sheet 24 is located under the gel 20 and provides a semi-rigid backing for the gel 20. The support sheet 24 preferably has a length and width at least that same as the gel 20. More preferably, the support sheet 24 has a length and width that extends past the ends of the gel 20 as shown in FIG. 2. The added dimensions on the support sheet 24 help to prevent damage to the edges of the gel 20 during sealing and shipment. A sheet smaller than the dimension of the gel is not desirable. The larger the gel (e.g., 20�25 cm), the more preferable it is to incorporate support sheets 24.
For example, testing has shown that for a 6�10 cm gel is best protected when centered on a support sheet 6.2�10.6 cm�1 mm, and a 10�15 cm gel is best protected when centered on a support sheet 10.2�15.6 cm�1 mm. However, it should be recognized that alternate sizes and shapes for the support sheet 24 are contemplated in the present invention depending on the size and shape of the gel and the users needs.
The support sheet 24 is preferably made from high impact polystyrene material or oriented polystyrene material with a thickness of about 10 mil. One or both surfaces of the support sheet 24 can be textured to minimize movement of the support sheet 24 within the package 10. In one preferred embodiment, the support sheet 24 is textured on the side facing the package 10 and smooth on the side adjacent to the gel 20. Testing has shown that the smooth surface adheres evenly to the gel 20 both when first placed onto the support sheet 24 and after over twelve hours of sitting in a sealed package 10. However, texturing of both sides is also contemplated if desired. It should be recognized that if additional stiffness is desired, an additional support sheet 24 can be incorporated on top of the gel 20. Alternatively, a thicker support sheet 24 can be used if desired.
The use of the support sheet 24 also permits the end user to easily remove the gel 20 from the package 10 since it provides a semi-rigid surface to grab or slide a spatula under. This is especially important for larger size gels 20 which would otherwise be very difficult to handle. If desired, the support sheet 24 as described above can be inserted with the gel 20 into a gel holder that is used to conduct the testing (not shown). Preferably the material from which the support sheet 24 is made has a density of greater than 1.0 to prevent the support sheet 24 from floating if used with the gel 20 during testing. Also, depending on the process used to initially insert the gel 20 into the package 10, the support sheet 24 can be used to facilitate such insertion. It is preferable that the support sheet material be ultraviolet light transparent to allow the user to photograph the gel 20 while on the sheet 24.
In an alternate embodiment of the invention shown in FIGS. 5 and 6A and 6B, a support tray 30 is incorporated into the package 10′ to further stiffen the package 10′ and protect the electrophoresis gel 20. In this embodiment, the gel 20 is preferably disposed or cast in the tray 30. The tray 30 includes a base 32 and at least two side walls 34 integrally formed with the base 32 and spaced apart from one another to receive a gel 20 therebetween. More preferably, there are four side walls 34 formed around the base 32. The tray 30 is designed to prevent pinching or crushing of the edges of the gel 20. More specifically, in a sealed package, the edges of the package angle toward one another at the seal. If the gel contained within such a package should migrate toward that sealed edge during shipping, the taper of the edges could cause crushing or pinching of the edge of the gel resulting in de-watering of the gel. Gels with such deformities can produce sample irregularities during use, such as sample migration.
To prevent this occurrence, the present invention incorporates a tray into one embodiment of the package 10′ which is designed to maintain the gel 20 at a uniform thickness until it is desired to be used. The tray also provides additional stiffness to the package 10′ to minimize bending of the gel 20.
A preferred tray 30 for use in the present invention is slightly larger that the gel 20 so as to provide a snug fit between the gel 20 and tray 30. However, in order to accommodate dimensional variations in the gel, the tray 30 is more preferably between about 1% to 2% longer than the gel 20, between about 1% to 2% wider than the gel 20, and has side walls 34 that have a height equal to (FIG. 6A) or slightly shorter than (between about 1% to about 5%) (FIG. 6B) the height of the gel 20 when the gel is placed within the tray 30. While the side walls 34 can be slightly taller than the height of the gel 20, doing so will reduce the contact between the top sheet 12 of the package and the top surface of the gel 20 when sealed. As such, the resulting constraint that such contact provides is lessened. It is not desirable to make the length or width of the tray shorter than the gel 20 since doing so will result in gel compression and deformation of the wells.
It is also preferable that the tray 30 not include any sharp corners which can damage the gel 20 or puncture the package 10′. Accordingly, in one embodiment of the invention (shown in FIGS. 6A and 6B), the package 10′ includes a small flange 36 around the top of the side walls and includes radii on all the corners. It should be recognized that thicker package materials could eliminate the need for radii on the edges and a flange.
The tray 30 can be formed from any suitable material. Preferably, the tray is made from a plastic material that is reasonably rigid. The rigidity of the tray 30 will vary depending on the material, tray size and thickness. Those skilled in the art would readily be capable of selecting a suitable tray material for use in the present invention in light of the teachings provided herein. In one embodiment, the tray thickness (i.e., the thickness of the base and side walls) is between about 0.010 inches to about 0.040 inches.
In the embodiment of the invention utilizing a tray 30, a support sheet 24 is generally not needed since the tray 30, in combination with the top and bottom sheets 12, 14, provides the necessary rigidity to prevent bending of the gel, and permits the gel to be readily extracted from the package.
As discussed above, the tray side walls 34 are preferably designed so that they are the same size as or slightly smaller than the gel 20 (FIGS. 6A and 6B). As such, the vacuum sealing of the package 10′ causes the top sheet 12 to apply a slight downward pressure on the top surface of the gel 20. The downward pressure on the gel 20 locks it into place within the tray, thereby inhibiting movement. The height of the side walls 34 prevent the pressure from the top sheet 12 from crushing the gel 20. As a result, movement of the gel 20 within the package 10 is minimized.
During development of the present invention, the inventors at first considered vacuum sealing to be inappropriate since it was believed that it would result in loss of moisture from the gel, and would cause the gel to become damaged from compression caused by the packaging. However, testing proved that the process did not cause any appreciable moisture loss and that the packaging materials conformed to the shape of the gel rather than crushing it. The addition of the tray greatly expands the capabilities and resulting rigidity of the package.
Another benefit provided by the packaging configuration according to the present invention is that the increased stiffness provided by the overlapping sheets 12, 14 allows thinner sheets to be used to form the package 10. This results in a packaging arrangement that is less expensive to manufacture. Furthermore, the thinner sheets prevent the gel 20 from being damaged during vacuum forming. For example, if thick sheets of material are used to form the package, they will resist bending when subjected to a vacuum force, more so than a thinner sheet. (The sheets bend to conform to the shape of the gel 20 when the air is evacuated from within the cavity.) Instead of bending, the thicker sheets tend to compress the gel 20. The use of thinner sheets in the present invention allows the sheets 12, 14 to conform more readily to the shape of the gel 20, thereby evacuating more air without damaging the gel 20. The problems described above with respect to thicker sheets may also occur with materials that have relatively high stiffness characteristics, such as polyethylene terephthalate (PET) material.
The incorporation of the tray into the package, on the other hand, permits thicker or stiffer sheets to be used, if desired, since the tray side walls will prevent the top sheet from compressing the gel. Alternately, since the tray adds considerable rigidity to the package, the bottom sheet may be thinner than the top sheet.
The present invention also contemplates the insertion of multiple gels 20 into the package 10. Referring to FIGS. 3 and 4, an embodiment of the package 10 is shown containing a series of stacked gels 20. FIG. 7A shows an alternate embodiment of the package 10′ containing a series of stacked gels 20 within a single tray 30. FIG. 7B illustrates an embodiment of the invention wherein multiple trays, each containing an associated gel, are stacked within the package 10′. In this embodiment, it is contemplated that locking ledges (not shown) may be formed on the bottom of the tray to secure the trays in a stack. The upper and lower sheets 12, 14 and tray of these embodiments are the same as discussed above except that they are larger to accommodate the added number of gels 20.
In order to prevent the gels 20 from adhering to one another, one embodiment of the present invention contemplates the incorporation of spacers 26 interleaved between adjacent gels 20. The spacers 26 are preferably made from high impact polystyrene material or oriented polystyrene material which provides a semi-rigid backing for the gel 20. The spacers 26 are preferably 10 mil thick and are textured on one side to reduce adherence of the spacer 26 to the adjacent gel 20. Although the spacer 26 can be textured on both sides, it is preferable that the spacer is smooth on one side to assist in adhering one gel (i.e., the upper gel) on the spacer 26. Testing has proven that the smooth surface adheres evenly to the gel 20 both when first placed onto the support sheet 24 and after over twelve hours of sitting in a sealed package 10.
The spacers 26 preferably have a length and width at least that same as the gel 20. More preferably, the spacers 26 each have a length and width that extends past the ends of the gel 20, but are less than the inner dimensions of the package (FIG. 3) or tray (FIG. 7A). The added dimensions on the spacers 26 help to prevent damage to the edges of the gel 20 during sealing and shipment. The larger the gel (e.g., 20�25 cm), the more preferable it is to incorporate spacers 26. In addition to separating adjacent gels 20, the spacers 26 permit the gels 20 to be removed one at a time from the package 10 since it provides a semi-rigid surface to grab or slide a spatula under. This is especially important for larger size gels 20 which would otherwise be very difficult to handle. If desired, the spacer 26 can be inserted with the gel 20 into a gel holder that is used to conduct the testing (not shown). Preferably the material from which the spacer 26 is made has a density of greater than 1.0 to prevent the spacer 26 from floating if used with the gel 20 during testing. Also, depending on the process used to initially insert the gel 20 into the package 10, the spacer 26 can be used to facilitate such insertion. It is preferable that the spacer 26 material be ultraviolet light transparent to allow the user to photograph the gel 20 while on the spacer 26.
If further rigidity is needed in the multi-gel embodiment, an end card (not shown) can be used on the top of the stack of gels. The end card is preferably made from plastic material, such as polystyrene or corrugated polyethylene. The end card has a preferred thickness of about 15 mils or more. In one embodiment of the invention, the end card thickness is selected from a range between about 15 mil and about 40 mil. The end card preferably has the same dimensions as described above with respect to the spacer 26. The end card may also be necessary in the single-gel embodiment when the gels are relatively large inasmuch as such gels can be damaged more easily from inadvertent crushing, or from contact with the pouch materials during vacuum sealing or placement and removal of the gel.
In order to form the package 10, the gel 20 is preferably first placed on the bottom sheet 14, and the top sheet 12 is placed on top. For the package configuration 10′ that includes a tray 30, the gel is first placed in (or cast in) the tray 30, then the combination is placed between the top and bottom sheets 12, 14. The package 10, 10′ is then placed in an evacuation chamber, such as a Ultravac� 2000 chamber, sold by Koch Supplies, Inc., Kansas, Mo. The air is evacuated from the chamber resulting in the air between the first and second sheets 12. 14 also being evacuated. Once a sufficient amount of air is evacuated so as to cause the edges of the sheets to overlie one another, the sides of the package 10, 10′ are sealed with a heat seal 16.
In the embodiment of the invention without a tray, a suitable package 10 was produced by operating the Ultravac� 2000 chamber with the following settings: Vac=98; Vacplus=0; Sealing=0.9; Cooling=5/99; and Venting: pulse from 99 to 0. A vacuum of about 27 inches of mercury is used in this embodiment of the invention, which is sufficient to evacuate a package 10 formed from 3 mil upper and lower sheets without damaging the gel 20. During testing it was determined that a vacuum of less than 20 inches of mercury was insufficient to produce a suitable package 10, but that a vacuum of about 23 inches of mercury or more would work well. Of course, a package 10 made from sheets of varying thickness may require more or less or a vacuum to properly evacuate the contained air and conform to the gel without causing damage. It is contemplated that other types of seals and method(s) of evacuating the air from within the package may be substituted for the disclosed method. It is also contemplated that other conventional vacuum sealing technique, such as gas flushing, can be used to form a package according to the present invention provided that the process is tailored to provide sufficient evacuation and sealing without damaging the gel.
It should be readily apparent that the process settings, such as vacuum pressure, length time of vacuum, cooling time, etc., would need to be adjusted depending on the type of vacuum chamber used, the physical characteristics and material properties of the gel, and the packaging material selected. The goal would be to tailor the settings so as to sufficiently evacuate the package 10 while preventing the gel from being crushed. Such modifications and alterations would be readily appreciated by one skilled in the art in light of the instant disclosure.
During testing it was determined that between 1% and about 89% of the air within the package 10 without the tray should be evacuated in order to immobilize the gel within the package 10. More preferably, between about 25% and about 40% of the air within the package should be removed to provide sufficient immobilization of the gel to prevent damage. Those skilled in the art, however, will readily appreciate that the desired amount air to be removed from the package will depend on the size of the gel (larger gels need more air removed because they are heavier and, therefore, are better protected when the package more closely conforms to the gel); the size of the package (a perfectly fitting package does not require the removal of much, if any, air); the stiffness of the package material (which will govern how easily the material drapes over the gel); and whether support sheets are used in the package (support sheets add protection, thus, lessening the amount of evacuation needed).
If the package 10′ includes a tray 30, a similar method as described above is carried out to form the package 10′. Between 1% and 99.9% of the air within the package should be evacuated in order to immobilize the gel within the package 10. More preferably, between about 25% and about 50% of the air within the package should be removed to restrain the gel and tray within the package. Once again, those skilled in the art will readily appreciate that the desired amount air to be removed from the package will depend on the various factors described above, as well as the stiffness and shape of the tray being incorporated into the package.
In order to protect the gel 20 from inadvertent damage during opening, the package 10 preferably includes indicia 28 (shown in FIG. 1) formed on the upper and/or lower sheet 12, 14 indicating where the user should cut to avoid contacting the gel. Preferably the indicia 28 instructs the user to open three sides of the package 10. In an alternative embodiment, one or more tear notches (not shown) may be formed in the package 10 providing the user with a easy method for tearing open the package 10.
The present invention provides a novel packaging configuration for protecting an electrophoresis gel from damage during shipment and storage. By vacuum sealing the gel within a durable pouch, the present invention minimizes movement of the gel that would otherwise cause damage. The incorporation of a tray into the package configuration further protects the gel from damage. While the above discussion has described the use of the invention with agarose gels, the present invention is suitable for use with all electrophoresis gels, such as polyacrylamide and similar polymeric materials. Also, while the present invention has applicability to a variety of gel thicknesses, it is particularly useful for gels between 2 mm to 7 mm thick since those gels are especially prone to damage during shipping.
Although the invention has been described and illustrated with respect to the exemplary embodiments thereof, it should be understood by those skilled in the art that the foregoing and various other changes, omissions and additions may be made therein and thereto, without parting from the spirit and scope of the present invention.
|Cited Patent
|Filing date
|Publication date
|Jul 1, 1980
|Feb 9, 1982
|Beckman Instruments, Inc.
|Electrophoretic gel container
|Jul 31, 1987
|Aug 9, 1988
|Bio-Rad Laboratories, Inc.
|Corrugated wedge spacers for slab gel molds
|May 10, 1991
|Feb 4, 1992
|Helena Laboratories Corporation
|Container for electrophoretic gel
|Jul 10, 1992
|Jun 8, 1993
|Macconnell William P
|Nucleic acid purification system and method
|Mar 7, 1994
|Jan 24, 1995
|Applied Biosystems, Inc.
|Notched spacer for slab-gel electrophoresis
|Dec 31, 1991
|Aug 22, 1995
|Fmc Corporation
|Electrophoresis gel container assemblies
|Jan 11, 1996
|Nov 17, 1998
|Methods for storage of sequencing gels and stored sequencing gels used by such methods
|Feb 17, 1999
|Mar 14, 2000
|C.C. Imex
|Package for electrophoresis gel
|Oct 23, 1998
|Jul 18, 2000
|Riley; Mary S.
|Vacuum package for electrophoresis gels
|Citing Patent
|Filing date
|Publication date
|Jul 19, 2005
|Feb 17, 2009
|Xiumei Sun
|Apparatus for use in gel electrophoresis
|Jul 19, 2005
|Jan 25, 2007
|Xiumei Sun
|Apparatus for use in gel electrophoresis
|U.S. Classification
|204/456, 204/606, 204/467, 204/618, 204/466, 204/616
|International Classification
|Cooperative Classification
|European Classification
|Jun 19, 2000
Owner name: CBM INTELLECTUAL PROPERTIES, INC., NEVADA
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:RILEY, MARY S.;PROVONCHEE, RICHARD;REEL/FRAME:010910/0038;SIGNING DATES FROM 20000530 TO 20000601
|Sep 18, 2006
|Fee payment
Year of fee payment: 4
|Oct 19, 2010
|Fee payment
Year of fee payment: 8
|Oct 14, 2014
|Fee payment
Year of fee payment: 12


text:
Kruger breaks the submission and stares at Zayn, who is now on the ramp. Kruger approaches the ropes. Kidd grabs Kruger from behind and pins him off the distraction for the win.
Winner: Tyson Kidd
Back from commercial and Sami Zayn vs. Leo Kruger is announced for next week.
Then, another WWE.com exclusive video is shown from last week where Hunico and Camacho are asked for their thoughts on beating the Ascension last week. They say that nothing has been given to them. They take what they want. Next week when they face the Ascension for the titles, they will take the gold.
NXT Championship
Bo Dallas (c) vs. Adrian Neville
They lock up and Neville locks in Bo's arm. They chain wrestle for a bit, and Bo rolls to the apron. Bo comes back inside and attacks Neville into the corner. Neville comes back with a crossbody and a dropkick. Bo rolls to the outside. He comes back inside and Neville backs him into the corner. Bo hits an elbow to the head. He charges Neville and hits a forearm as the middle turnbuckle pad falls off. Bo stomps Neville and locks in a cravat. Neville gets to his feet, but Bo power whips him into the corner. He whips Neville into another corner, hits a clothesline, and lands a bulldog.
Neville kicks out at two. Bo lands a body slam. Bo exposes his knee and climbs up top. He yells don't stop bolieveing and leaps off. Neville rolls out of the way. He kicks Bo in the knee repeatedly. Neville lands two leaping forearms and a kick to the face. He dropkicks Bo in the face, and Bo rolls to the outside. Neville takes him out with a dive. He sends Bo back in and gets a near pin fall. Neville lands a body slam and goes up top. Bo rolls to the outside. Neville takes him out with a crossbody. They both writhe around on the floor in pain. They both crawl to get in before the ten count, and at nine Neville slides in and Bo drops to the floor.
Winner via count out: Adrian Neville
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Home Ministry upset with MEA over EU envoys visit
New Delhi: The Ministries of Home and the External Affairs were on Monday at loggerheads over the visit of eight envoys of the European Union to Nagaland.
Following a protest lodged by MHA over the visit without any intimation and security clearance and the ministry raising its concern on the delegation meeting political leaders and civil society, Foreign Secretary Rajan Mathai spoke to Home Secretary RK Singh on the issue.
"In all such visits necessary process is ensured and coordination is ensured amongst all concerned organisations and ministries," sources in the MEA said.
Asserting that the decision to allow the diplomats to visit the sensitive northeastern state was "unilateral", the MHA asked the MEA to tell it the purpose of the visit and wanted to know why security clearance has not been sought before giving permission for the visit as is the normal practice.
Official sources said the delegation, which includes ambassadors of Czech Republic, Hungary, Finland, Poland, Bulgaria and Slovakia, met Nagaland Chief Secretary, Speaker of the Assembly, leaders of political parties, representatives of several NGOs and businessmen on Sunday.
The envoys also visited Nagaland University and met Governor Nikhil Kumar today. Chief Minister Neiphiu Rio is hosting a dinner for them on Monday night.
Sources said the MHA was particularly upset over the delegation`s meeting with leaders of political parties and civil society members.
It was of the view that Nagaland being an insurgency-prone state such representations could lead to sharing of information which are inimical to country`s interests.
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text:
School denies mother's claim of assault on sons by teacher
The Swami Purnananda Secondary has denied allegations by a Mahaicony Creek mother who said her two sons were beaten by a karate teacher at the school.
A letter to the Stabroek News, written by the Chairman of the school's management team Ramnauth Ramsarran, stated that last Thursday a complaint was received from a parent, who claimed her son was punished by a karate teacher.
The school said the parent was immediately informed that the school does not offer karate on its curriculum and did not have a karate teacher.
The mother, Debbie Persaud, said her 10-year-old and 11-year-old sons were beaten in the school's dormitory by the teacher in the presence of the caretaker of the dormitory. She had stated that her youngest son did not attend the school but stayed in dormitory since their Mahaicony home was far away.
Persaud said she will take legal action against the teacher and was preparing documents to have her sons transferred. She had also made a report of the assault to police.
However, the school stated that at no point was her son punished by any teacher in the school.
The school said that as a policy it does not institute corporal punishment on pupils. "Any disciplinary action that needs to be taken is discussed first with the parents, the Parent Teacher Association and then the school's Management Committee," the letter stated.
It further said that the school has no control of any other activities, including karate, a student may be involved in outside of normal school hours.
Persaud, however, said that she received a call from the caretaker of the dormitory and he was upset because she had reported the issue to the media and the police. She said the man told her that her son was a troublemaker and that he had stolen money from the school.
She said her son was still in pain from the beating he received. "My son ain't well. He hollering with pain and the (teacher) calling and telling me that he was misbehaving," she said.
"He is mad with me because I put his name in the papers and the TV but the truth is my sons get kick up and was beaten mercilessly," she said.
Persaud's elder son is a student at the Swami Purnananda Secondary School while the younger one attends the St. Andrews Primary school.
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February 6, 2013
"Boneta Bill" easily passes Va. House, heads to Senate
Video/Fauquier Pitchfork
Supervisor Lee Sherbeyn testifies against HB 1430 before House subcommittee.
Martha Boneta has filed a lawsuit against Fauquier County because of the zoning dispute related to her farm market at Paris.
Despite opposition from Fauquier and other farming counties, the Virginia House of Delegates this week overwhelming passed the "Boneta Bill" to expand the state's "Right to Farm Act."
Introduced by Del. Scott Lingamfelter (R-31st/Woodbridge), who represents the New Baltimore area of Fauquier,
passed the House, 77-22, Monday morning.
The two other delegates representing parts of Fauquier — Michael Webert (R-18th/Marshall) and Mark Cole (R-88th/Spotsylvania) — also voted in favor of the bill.
Of the 22 no votes, only one came from a Republican, Del. Randy Minchew of Leesburg.
The proposed legislation moves to the Senate.
The bill seeks to expand the definition of agricultural operations to include the sale of products to consumers and businesses. It would prevent counties from classifying activities as non-agricultural without providing sufficient proof.
"If you're farming, you're farming. And if someone comes along and says you are not farming, the burden is on them to prove that you are not farming," Del. Lingamfelter said.
HB 1430 has a re-enactment clause, meaning it would not take effect unless the 2014 General Assembly also passes the legislation.
The bill draws its name from
Martha Boneta
, a Paris (Va.) farmer. Last year, she got into a dispute with Fauquier County officials because she was selling handicrafts and holding events at her farm without proper permits. Ms. Boneta's supporters demonstrated with pitchforks – first in Fauquier County and last week at the state Capitol – to protest what they see as government interference in farmers' activities.
The Boneta Bill has put Del. Lingamfelter at odds with the Fauquier County Board of Supervisors, with four of five members also Republicans.
Supervisors Lee Sherbeyn (R-Cedar Run District) and Holder Trumbo (R-Marshall District) have testified against the bill in committee hearings.
Boards of supervisors in Clarke, Rockingham and Shenandoah counties also have adopted resolutions opposing the bill. Additionally, the Virginia Farm Bureau opposes it.
The General Assembly session will end Saturday, Feb. 23.
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