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Twitter Link Facebook link
As UK church leaders prepare for a pilgrimage to the beleagured city, news has emerged that a peacebuilding school in Bethlehem which welcomes Muslim, Christian and Jewish girls and boys has been told that part of their building is due to be demolished by the huge Israeli 'security wall' being constructed around the town.
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Early this morning (27 June 2016), members of Christian peace groups blocked the three entrances to AWE Burghfield using super glue and arm tubes...
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subset: commoncrawl


text:
New Builds • April 23, 2015
Novatel Launches Multi-GNSS Wideband Antenna
NovAtel Inc. today (April 23, 2015) launched the GPS-704-WB antenna, a wideband antenna that supports L-band as well as the frequencies of all current GNSS constellations (GPS, GLONASS, Galileo, and BeiDou), including B3 and E6 signals. Customers can use this antenna for GPS-only or multi-constellation applications, providing excellent flexibility for application developers, according to the company.
Ventures • April 16, 2015
ComNav and CGEOS Build First BeiDou CORS Station in Europe
A BeiDou continuously operating reference station (CORS) station, reportedly the first such commercial venture in Europe, has begun operations in Wallonia, Belgium.
April 14, 2015
GNSS Markets, Standards, and Resilience at the European Navigation Conference
The 2015 edition of the European Navigation Conference (ENC) in Bordeaux last week found the continent's GNSS community leaving troubles behind and looking to the future, where the world's dependence on GNSS is greater, the risks higher, and the potential profits ever more tantalizing.
New Builds • April 15, 2015
Septentrio Introduces New-Generation GNSS OEM Receiver; SBG Selects AsteRx4 for New INS Product Line
Septentrio has launched its next-generation dual-antenna GNSS receiver — the AsteRx4 OEM, a multi-frequency receiver that incorporates the latest GNSS tracking and positioning algorithms and operates with all available GNSS signals (GPS, GLONASS, BeiDou, Galileo).
March 31, 2015
China Launches New-Generation BeiDou Satellite
China's Xinhua news agency has announced the launch a new-generation BeiDou satellite at 9:52 p.m. Beijing time yesterday (March 30, 2015).
This is the first of five next-generation BeiDou spacecraft that China will launch this year, Jianyun Chen, a deputy director of the China Satellite Navigation Office (CSNO), told a Munich Satellite Navigation Summit last week.
March 25, 2015
GSA's GNSS Market Report #4: The Most Thorough Yet
The European GNSS Agency (GSA) unveiled its latest GNSS Market Report today (March 25, 2015) at the Munich Satellite Navigation Summit in Germany.
The fourth and most comprehensive in a series of GSA studies, the 80-page report describes current and projected GNSS receiver sales by volume and value and highlights market opportunities, technology trends, and future developments of GNSS.
March 22, 2015
u-blox Announces Profit, Revenue Advances in 2014
Thalwil, Switzerland–based u-blox reported revenue and profit growth in its most recent annual report, driven by strong sales in its GNSS and wireless chipset and module business.
March 14, 2015
Renewed Spectrum Fight Emerges between GPS, LightSquared at ABC Workshop
Spectrum-related tensions reemerged during a workshop on Thursday (March 12, 2015) organized to gather feedback on a testing plan to help protect GPS receivers.
The plan is part of the GPS Adjacent-Band Compatibility (ABC) assessment — a wide-ranging effort to determine the power levels at which services operating in frequencies next those used by GPS and other GNSS systems can broadcast signals without causing interference to GPS signals.
Inside GNSS • March/April 2015
GNSS and Precision Farming
Nowhere has the fact that GNSS can guide things besides military weapons and transport manifested itself more profoundly than in agriculture.
While Google and automotive manufacturers struggle to figure out how to put autonomous vehicles on the highway, farmers have been using GNSS for well over a decade to guide equipment through their fields — along with a host of other ag-related, site-specific applications.
Indeed, GNSS — along with an array of other high-tech resources — is transforming agriculture at an accelerating rate.
GNSS Solutions • March/April 2015
How does a GNSS receiver estimate velocity?
Q: How does a GNSS receiver estimate velocity?
A: Stand-alone single-frequency GNSS receivers represent the largest slice of the commercial positioning market. Such receivers operate mainly in single point position (SPP) mode and estimate velocity either by differencing two consecutive positions (i.e., approximating the derivative of user position) or by using Doppler measurements related to user-satellite motion.


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EDIT: Power Gem looks great. 20 28 not so much. A6 stays. Dragon Pulse is supposed to be a lightish purple, but since I couldn't find one I guess it should stay dark.
I found those a long time ago Personally I think it's too much work to resize them since most of them won't be used.
Thanks. Yeah I was about unsure about 20 28 myself, but I was just looking at the BW animation to yeah it's all good. I also come bearing a gift. A fire background that can scroll in any direction you want :D


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Azoi introduces Wello health monitoring iPhone case
New accessory maker Azoi has announced Wello, a shell-style case for iPhone 5/5s with health monitoring capabilities. With its combination of sensors and electronics, the case can track blood pressure, electrocardiography (ECG), heart rate, blood oxygen, temperature, lung functions, and other vital statistics.
According to the company, simply holding the case for a few moments is all that's needed for it to register the information, which is then transferred to a companion app. Wello is currently available for pre-order, and will sell for $199. It's slated to be released in fall 2014, pending FDA approval.
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Recent News
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- Filling the Gap: A look at third-party HomeKit apps
- Instant Expert: Secrets & Features of tvOS 9.2
- Instant Expert: Secrets & Features of iOS 9.3
- Opinion: Why Apple needs a dedicated HomeKit app


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Wrong ... advertising does not need to be costly.
Couple of billboards will ignite the fire storm.
Couple of radio announcements will ignite the fire storm.
Couple of TV interviews will ignite the fire storm.
Couple of press releases will ignite the fire storm.
Which one has MNKD done so far? NONE!
Sentiment: Strong Buy
All excellent points!
Sentiment: Strong Buy
If it's a BK then why give additional shares to the staff recently?
Their reluctance to advertise makes me suspect we are either heading to a BO or a BK.
I was wondering the very same.
Good tweet but will MNKD respond?
Compensation should be based on accomplishment.
You didn't notice the knife in your back?
Sentiment: Strong Buy
Just a curiosity.
Stock_Fanthom has a long position.
New partnerships will be approaching on our terms from now on.
$50/share is now within reach.
Sentiment: Strong Buy
All during that period who was in the picture?
Are they still in the picture?
Sentiment: Strong Buy
Uh huh ... Losses resulted from trusting the stinky Frenchmen.
Do you disagree???
Sentiment: Strong Buy
Matt, start your advertising campaign so we can start seeing green.
Good luck to all longs!
Sentiment: Strong Buy
I work the late shift ... why are you here at this hour hun?
I will give you a thumbs up to get your attention.
Sentiment: Strong Buy
Wow, look at all the nervous thumb downs at middle of night.
Certainly did not expect to see this.
I guess short positions can't sleep tonight,
Sentiment: Strong Buy
Just a midnight curiosity.
Sentiment: Strong Buy
SNY got their milestone payments back by shorting MNKD.
Now that relationship ended, they need to buy shares to cover.
Not only their massive shorting ended but now they will drive the PPS up when they cover.
Only concern is will MNKD be able to keep the doors open until Afrezza hits the market.
Sentiment: Strong Buy
Are you satisfied with your shareholder interest protection on your watch???
Good day for you to hide under your desk.
Many of your longs don't approve of you occupying that office.


text:
OK, Tennessee gun nuts: We know how much you love your guns. I mean really love your guns. So much that you want to take them to bars. To parks. Even to Belle Meade.
Above, we present to you, the first official Nashville Scene-sanctioned gun for bars, parks, cookouts, cocktail parties, debutante balls, you name it. Take it anywhere you like! As long as you have about $6 million to spare.
Showing 1-7 of 7


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"Justice League": Exploring How Superman Returns (Again)
Film, Comic Books
Comic Books
Comic Books
The Buy Pile
The Buy Pile
In Your Face Jam
Robot 6
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Comics Should be Good
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Comics Should be Good
Robot 6
Jun 27, 02:08am
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Jun 27, 00:42am
Portage, IN
As part of their ongoing promotion of Jeff Smith's first children's book, Little Mouse Gets Ready, publisher Toon Books has made an exclusive plush doll, created by dollmaker Sabrina Cho. It retails for $75 and comes with a certificate of authenticity and a copy of the book signed by Jeff Smith. Also: It's too cute for words.
Found via Comics Worth Reading
I picked up this book at my LCB (not the dolly) for my three year old – I loved it, but he didn't! He objected to the ending – it seemed the story ended with the little mouse in trouble (his interpretation, not the book's) But he did love Speigelman's Jack and the Box – lots of laughs!
Home | News | Columns | Reviews | Video | Blogs | Forums | Find A Comic Shop
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text:
Cheesy, Creamy, Twice Baked Potatoes
The title says it all! People have been known to burn their tongues on these because they just couldn't wait! Vegetarian's can skip the bacon or use imitation...
potato, butter, heavy cream, black pepper, salt, +bacon, +cheddar cheese, +cream cheese, +fontina cheese, +green onion, +romano cheese, +sea salt, +sour cream
Blue Cheese Potato Gratin
black pepper, heavy cream, blue cheese, potato, salt, +whole milk, +olive oil, +onions
Gratin Dauphines
From More Food for Thought
potato, butter, heavy cream, black pepper, salt, +garlic, +milk, +swiss cheese
Colombian Chicken, Corn, and Potato Stew
black pepper, heavy cream, potato, salt, butter, +california, +chicken broth, +capers, +oregano, +corn, +yukon gold, +water, +onions
Potato Gratin with Sage and Onions
black pepper, salt, butter, heavy cream, potato, +garlic, +red onions, +water
Potato Gratin with Cream and Fresh Herbs
butter, potato, heavy cream, salt, black pepper, +whole milk, +chives, +thyme
epicurious.com - Similiar recipes
Vichyssoise Recipe
black pepper, heavy cream, potato, salt, butter, +chicken broth, +leek, +onions
www.cdkitchen.com - Similiar recipes
Comforting Chicken Recipe
www.cdkitchen.com - Similiar recipes
Bacon and Onion Potato Casserole
A friend invited me to a collaborative dinner and said my task was to bring a cool potato dish. This one fit the bill and was easy to transport!
butter, heavy cream, black pepper, salt, potato, +bacon, +onions, +parmesan cheese
Blue Cheese and Bacon Potato Skillet
This recipe may easily be doubled, all ingredient amounts may be adjusted to taste, you may omit the garlic if desired
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text:
1 of 3 people found the following review helpful
Good quality live recording,
This review is from: Live in Montreux (Audio CD)
This live CD features Gilberto playing his more famous jazz samba masterpieces, including the Girl from Ipanema.
The quality of the recording is high and captures the atmosphere of the festival. However, the performance is only Gilberto unaccompanied apart from his guitar. Also, all the renditions are in the original Brazilian.
It is a good CD for completists but not beginners. Start with Jazz Samba, Jazz Samba Encore and Getz/Gilberto Vol 1 and 2.
Review Details
(2 customer reviews)
Location: London, UK
Top Reviewer Ranking: 46,167


text:
Russia votes against UNHRC resolution placing all blame on Syrian governmentRussian Politics & Diplomacy July 01, 21:42
Russia's investment relations with Turkey will be restored — ministerBusiness & Economy July 01, 21:30
Finnish president notes role of sanctions in Finland-Russia trade declineBusiness & Economy July 01, 20:14
Russia will tighten responsibility for doping violations — PutinSport July 01, 19:45
Putin: Russia to respond if Finland joins NATORussian Politics & Diplomacy July 01, 19:35
IAAF suspends Russian high jumper Chicherova for doping probeSport July 01, 19:31
Russia and Finland agree to use tranponders during flights over Baltic regionWorld July 01, 19:16
Elaboration of new edition of Russia's foreign policy concept nearing completionRussian Politics & Diplomacy July 01, 18:46
Kamchatka, Russia's land of volcanoesSociety & Culture July 01, 18:13
{{element.title}}{{element.heading}} {{element.date}}
MOSCOW, October 1. /TASS/. Russian President Vladimir Putin has ordered measures to protect Internet's Russian segment.
"It is obvious that today we need to develop and implement a range of additional measures in the area of information security," the head of state said at a meeting of Russia's Security Council. Such measures should focus on enhancing the protection of the Russian communication and information networks, primarily those used by government agencies, Putin said. It is important to ensure the stable and safe operation of the Russian segment of the Internet, the president noted.
Putin has confirmed that websites with illegal content will be closed down. "We shall be acting systematically and in strict compliance with the law and close down such sites (with prohibited content)," Putin said on Wednesday. However, Russia will not limit access to Internet and subject it to control, he stressed.
The Russian president said some countries are taking advantage of their leading position on the Internet to reach military and political aims. "We see that certain countries are trying to use their dominating position in the global information space to achieve not only economic, but also military and political goals," the Russian leader noted.
Russia has registered an increase of hacker attacks on Russian information web sources over the course of the past six months, Putin said. "Our corresponding departments are registering the constant growth of web attack on Russian information resources and in the course of the past half a year, the number of attacks grew. This is simply incomparable with last year," Putin stressed.
The Russian president said that methods, tactics and ways of web attacking were constantly upgraded and improved. "The intensity (of attacks) directly depends on the current international situation," Putin said.
It is also essential to expand cooperation in ensuring international information security with global and regional organizations, the Russian president said. "I believe the United Nations Organization, its specialized groups and structures should become a site for assessing risks and elaborating joint measures in the area of information security and analyzing the legal consequences of decisions that are made," Putin said.


text:
|Publication number
|Publication type
|Application number
|Publication date
|Jul 15, 1997
|Filing date
|Jul 19, 1996
|Priority date
|Oct 17, 1994
|Fee status
|Publication number
|Jack H. Kessler, James C. Richards
|Original Assignee
|Symbollon Corporation
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (9), Non-Patent Citations (9), Referenced by (34), Classifications (11), Legal Events (5)
|External Links: USPTO, USPTO Assignment, Espacenet
This application is a continuation of application Ser. No. 08/324,391, filed Oct. 17, 1994, now abandoned.
The present invention relates generally to the enzymatic formation of microbicide compositions that contain (1) a minimum level of free molecular iodine of above 10 ppm and a maximum level below 330 ppm and (2) defined ratios of free molecular iodine to other iodine species in order to inactivate pathogenic organisms. More specifically this application contemplates the use of an enzymatically generated iodine microbicide to inactivate pathogens that are contaminants in sensitive biological materials. A wide range of sensitive biological materials are contemplated under this application. These materials include cell lines used for tissue culture, whole blood, red blood cells, blood plasma, blood proteins, human cells such as sperm, human tissues such as skin and human organs. In order to be useful, a liquid microbicide should inactivate all unwanted pathogens and not substantially reduce the biological activity of the sensitive biological material of interest. The biocidal agent from the enzymatic reaction is free molecular iodine generated by the reaction of peroxidase, iodide and hydrogen peroxide.
Sensitive biological products from human and animal sources are widely used for therapeutic, diagnostic and experimental purposes. Such products are subject to contamination by virtue of their natural environment which presents favorable conditions for microbial contamination and subsequent handling. Frequent contaminants include bacteria, viruses and fungi. Diagnostic tests for the presence of such pathogens are available but there has been no way to assure that these products are free from unwanted contaminants. A good example of this is the transfusion of human blood products which currently carries a well-known risk of transmitting a number of viruses and bacteria.
A large variety of chemical germicides have been used in the medical profession and biomedical industry to inactivate unwanted bacteria, viruses and fungi. The majority of these chemical germicides are either toxic or destructive to organic material and, as a result, these disinfectants are not suitable for use with sensitive biological materials. U.S. Pat. No. 5,185,371 describes a method to disinfect blood products that relies upon diluting a germicide in one of the following compositions: 0.9% aqueous sodium chloride, 5% aqueous dextrose, sugar solutions or other diluents that ameliorate the chemical incompatibilities of the liquid germicides with blood and blood products.
F. A. Highsmith et al. (Journal of Infectious Diseases, volume 167, page 1027, 1993) describes methods that utilize a chemically modified polyvinylpyrrolidone-iodine complex. The compositions and methods described in Highsmith have been used to disinfect blood products. The intuitive appeal of using iodine for such applications is obvious since iodine is a nutritional additive.
All of the prior art related to decontamination of sensitive biological materials with iodine containing compositions utilizes povidone-iodine or other complex chemical combinations that contain iodine either in the presence of high molecular weight polymers or bound to larger molecules. However, the prior art does not directly address the critical parameters required to effectively utilize iodine for the disinfection of biological materials. The key to effective use of iodine with sensitive biological materials in accordance with the present invention is to be able to control the level of free molecular iodine such that free molecular iodine is the principal iodine species present and to control the ratio of free molecular iodine to the other species of iodine present. In addition, it is highly preferred to substantially maintain the level of free molecular iodine under the conditions of pH that are not destructive to the biological materials.
In order for free molecular iodine to be effective, the level of this biocidal agent must be substantially maintained for a period of time that is sufficient to inactivate all of the unwanted pathogens. However, the chemical activity of free molecular iodine must be controlled in order to substantially maintain the integrity of the biological material of interest. The conflicting attributes of biocidal activity and biological compatibility require the practitioner to eliminate or minimize (1) other iodine species present which will contribute toxicity and chemical incompatibilities and do not necessarily contribute biocidal efficacy and (2) other non-biocidal chemicals since they serve as a potential source of contamination and incompatibility.
The pH that is most compatible with the overwhelming majority of biological materials contemplated under this application is pH 6.8 to 7.0. While it is not necessary to decontaminate the biological materials of interest precisely at a pH of 7.0, it is highly preferred to maintain a pH as close to 7.0 as possible to minimize the potential reduction in biological activity of the molecules of interest. However, a neutral pH introduces an additional factor that complicates the use of free molecular iodine in disinfection. Free molecular iodine is not stable at a neutral pH and, as a result, the duration of treatment is a critical parameter.
One of the disinfectants identified in U.S. Pat. No. 5,185,371 is 10% povidone-iodine which is frequently represented as PVP-I2. PVP-I2 is a complex of iodine with povidone. PVP-I2 contains less than 9.0% by weight, and not more than 12% by weight of thiosulfate titratable iodine. In addition, PVP-I2 typically contains not more than 6.6% by weight of iodide ions. Iodate and other inorganic species are typically added to PVP-I2. As a result, PVP-I2 contains all of the most common iodine species that are found in an aqueous environment including free molecular iodine, iodate, iodide, triiodide and hypoiodous acid. PVP-I2 is available commercially from many different manufacturers and the pH of these products will vary by manufacturer. However, PVP-I2 is strongly buffered at acidic conditions that typically range between pH 3.5 and 4.5.
The distribution of iodine species in PVP-I2 does not favor its use for sensitive biological materials. PVP-I2 is not a preferred degerming agent for biological materials since less than 0.2% of the iodine contained in such compositions is in the form of free molecular iodine. In addition, the pH of these compositions are toxic to most of the cells and tissues contemplated under this application. PVP-I2 was designed to be applied directly to intact skin and the FDA approved use pattern is limited to intact skin. The epidermis of mammalians consists of dead cells that are significantly less sensitive to harsh chemicals than living cells.
U.S. Pat. No. 5,185,371 describes the treatment of blood and blood products with PVP-I2 at concentrations that range from 0.01% to 0.5% by weight for up to 5 minutes.
This application proscribes a concentration range for PVP-I2 that corresponds to a 1/200 to a 1/10,000 dilution of PVP-I2. When PVP-I2 is diluted in normal saline at 1/200 the pH of the resulting solution is≈4.6 and the level of free molecular iodine is≈10 ppm When PVP-I2 is diluted in normal saline at 1/10,000 the pH of the resulting solution is≈5.4 and the level of free molecular iodine is≈0-0.5 ppm. The combination of free molecular iodine, pH and contact time described in U.S. Pat. No. 5,185,371 is not adequate to effectively inactivate most of the bacteria and viruses of interest in the presence of a competing bioburden. It is known that a significant amount of iodine and/or extended contact times are required to completely inactivate non-lipid-enveloped viruses (Highsmith F. A. et at., Transfusion, volume 34, page 322, 1994).
Accordingly, there presently is a need to provide an iodine based method to disinfect sensitive biological materials including cell culture, human blood products, animal blood products and human and animal tissue products. Iodine based compositions have been formulated to disinfect sensitive biological materials but these compositions have several drawbacks. Iodine is an effective biocide and unlike other biocides, iodine is required for proper human nutrition and thyroid function. The difficulty with previous iodine based compositions is that they have unfavorable characteristics of pH and ionic strength and that active biocidal iodine (i.e., free molecular iodine) is (a) combined with other chemical species that are not biocidal and (b) not the predominant iodine species.
The foregoing object of the present invention is accomplished by generating an iodine based microbicide in which there is a minimum level of free molecular iodine above 10 ppm and which comprises from 10% to 98% of the iodine species present on a molar basis. Another way to state this is that the iodine compositions will contain a plurality of iodine species with the molar ratio of free molecular iodine to other iodine species ranging from 10/100 to 98/100 and in a concentration range between 10 and 330 ppm. The preferred molar ratio of free molecular iodine to other iodine species should be above at least 25% and up to 98%. Such an iodine composition can be formed in an aqueous environment that is maximally compatible with a given biological material and will not only disinfect biological products but will do so without damage to the material of interest such as blood cells and tissue cells. It is presently believed that the disinfectant compositions of the present invention will render sensitive biological materials, e.g., tissue culture cells, safe, i.e., inactivate harmful bacteria, viruses and virus-like agents that may be present.
It is well known to those skilled in the art of tissue culture that mycoplasma organisms can contaminate cell cultures and can produce significant changes in tissue culture cells such as altered morphology, extended cell cycle and reduced output of specialized cell products such as monoclonal antibodies. Mycoplasma cells are distinguished from most other bacteria because they do not possess a cell wall. They are often found attached to the outer surface of a tissue culture cell membrane and in heavily contaminated cell cultures hundreds of mycoplasma organisms can be found coating the entire cell plasma membrane surface.
"Curing" a cell line from mycoplasma contamination refers to an effort to eliminate mycoplasma from tissue culture. A broad range of methods to eliminate mycoplasma contamination have been published including in vivo passage of tumor cell lines in nude mice, co-cultivation of infected cells with macrophages with or without antibiotics, induction of chromosomal damage in mycoplasma using 5-bromouracil and visible light, supplementation of culture media with specific antisera against mycoplasma and antibiotic treatment. It should be pointed out that experts in the field often recommend discarding mycoplasma contaminated cells whenever possible because the current cures are ineffective, impractical, cytotoxic or negatively affect the properties of cell lines. Nevertheless, the technician has no options in many cases because mycoplasma infected cells are often unique and irreplaceable. A method to cure infected cells lines from mycoplasma infection that does not generate resistance would be of great utility to the scientific community.
Iodine based germicides are well known in the art. The method used to generate the iodine based germicide of this application is to use peroxidase in combination with iodide and hydrogen peroxide. It is known from Kessler (U.S. Pat. Nos. 4,227,161, 5,169,455, 4,996,146 and 4,937,072), Orndoff (U.S. Pat. No. 4,370,199) and Montgomery (U.S. Pat. No. 4,576,817) that a combination of peroxidase, peroxide and iodide anions will form a bactericide in an aqueous environment. The bactericidal efficacy of this combination results from the enzymatic reaction that occurs when peroxidase, hydrogen peroxide and iodide react in solution. Peroxidase is known to effect the transfer of electrons from iodide to hydrogen peroxide. Hydrogen peroxide is converted into water by this reaction. Several possible reaction products have been postulated for the iodide anion including: 1) iodine free radicals (Nunez and Pommier, European Journal of Biochemistry, volume 7, pages 286-293, 1969); 2) hypoiodite ion (Morrison and Schonbaum, Annual Review of Biochemistry, volume 45, pages 861-888, 1976); and 3) iodinium ion (Ohtaki, Nakagawa, Kimura and Yamakazi, Journal of Biochemistry, volume 256, pages 805-810, 1981).
It has been observed that under certain conditions these peroxidase reactions will generate significant levels of free molecular iodine. The phrase "free molecular iodine" is a term of art that refers to elemental iodine, the chemical species that is represented as I2 and that is capable of being titrated with sodium thiosulfate. It has been observed that it is possible to generate defined levels of free molecular iodine across a wide pH range using compositions comprised of peroxidase, peroxide and iodide. The exact level of available iodine in these compositions is a function of the concentration levels of peroxide, iodide, peroxidase, buffering agents, pH and other additives.
It is understood that if a material like red blood cells are to be disinfected, the disinfectant composition must be substantially isotonic so as to eliminate the potential for damage to the red blood cells. The disinfectant concentration and the time required to effectively inactivate any pathogenic organisms is dependent upon the level of free molecular iodine in the final environment. Suitable concentrations of free molecular iodine are a function of the precise biological material of interest. In general the range of free molecular iodine that is suitable is between 10 and 330 ppm. The amount of contact time required to inactivate the pathogenic organisms of interest is a direct function of the level of free molecular iodine, the pathogens of interest and the level of biological material in the composition.
After contacting the sensitive biological material of interest with free molecular iodine, the free molecular iodine is allowed to dissipate via hydrolysis in the composition. Alternatively, the iodine in the composition can be separated by a plurality of means that are familiar to one skilled in the art including adding materials, like cyclodextrin, that bind iodine or passing the composition of columns that contain iodine binding materials.
A method is provided in which most types of pathogenic organisms in presence of sensitive biological material are completely inactivated. Additionally, the biological materials may be used subsequently for therapeutic or diagnostic purposes. The invention is based, in part, upon the unexpected discovery that elevated concentrations of free molecular iodine are compatible with many sensitive biological materials including, but not limited to, red blood cells, leukocytes, platelets, human embryonic lung cells, HeLa, Hep-2 cells, BSC-1 cells, WI-38 cells, CHO cells and astrocytes.
In accordance with this invention free molecular iodine is generated and contacted with the biological material of interest. The level of free molecular iodine that is required to be in contact with the contaminated material is between 10 and 330 ppm. The compositions contemplated under this application will have a molar ratio of free molecular iodine to other iodine species that ranges from 10/100 to 98/100. In order to optimally convert iodide into free molecular iodine the ratio of hydrogen peroxide to iodide is preferably established with a molar ratio that is less than 4; the optimal molar ratio of hydrogen peroxide to iodide that can be initially established is a 1/2 ratio. The pH of the aqueous environment must be controlled to be between pH 5.5 and 7.5. The preferred pH range in which to treat the biological material of interest is between pH 6.0 and 7.0. The contact time for the free molecular iodine can be varied widely and will depend upon the level of organic material in the composition and the temperature at which the material is treated. The contact time and temperature of treatment must be derived empirically for each application.
Free molecular iodine is generated by using peroxidase, iodide, and a source of hydrogen peroxide. It is obvious that the concentration of free molecular iodine used must be adequate to inactivate the contaminating pathogens; however, what is less obvious and equally critical is to generate an iodine based germicide whose principal component is free molecular iodine. By contacting the material of interest with an iodine composition that is principally free molecular iodine one minimizes the potential for chemical incompatibilities and contamination. Free molecular iodine should comprise at least 10% on a molar basis of all of the iodine species present after the iodine based germicide has been placed in an aqueous environment with the biological material of interest.
The reactants that comprise the peroxidase catalyzed oxidation of iodide must be controlled to yield a suitable result. The reaction should be established so as to efficiently convert iodide ions into free molecular iodine. Additionally, it is preferable to establish reaction conditions that convert at least 50% of the initial concentration of hydrogen peroxide into water and in no event should the concentration of hydrogen peroxide that is in contact with the biological material of interest be above a level of 0.015%. In order to optimally convert iodide into free molecular iodine the molar ratio of hydrogen peroxide to iodide should be initially established in a 1/2 proportion. It is highly preferred to limit this ratio to no greater than 4.
The preferred oxidant of this invention is hydrogen peroxide. Any material which acts as a source of hydrogen peroxide when admixed in an aqueous environment is suitable for the present invention. The term "source of peroxide" for purposes of the present invention and as used herein shall mean any material alone or in combination which can serve as precursors for hydrogen peroxide including metal peroxides, percarbonates, persulphates, perphosphates, peroxyesters, urea peroxide, peroxyacids, alkylperoxides, acylperoxides and perborates. Alternatively methyl peroxide can also be used as a source of hydrogen peroxide. Mixtures of two or more of these substances can also be used. The concentration range .for hydrogen peroxide in the final composition is between 0.005 and 0.025% in the final composition prior to initiation of the oxidation of iodide. The preferred concentration for hydrogen peroxide is between 0.001 and 0.01% in the final composition prior to initiation of the oxidation of iodide.
The donor molecule of this invention is iodide anion. Suitable dry sources of iodide anion for this invention include sodium iodide and potassium iodide as well as other salts of iodide. Any compound which yields iodide anion upon dissolution in an aqueous environment is suitable for this application. The simple salts of iodide are preferred and have the advantage of being less costly. Additionally, they have a long shelf life in solid form.
Iodide anion can be provided to the system in a liquid form if it is kept stable prior to use. Specifically, it is preferred not to contact the iodide anion with hydrogen peroxide. The concentration iodide that will yield a suitable level of iodine varies with the pH of the contemplated formulation. In addition, the required iodide level will vary dramatically depending upon the ratio of peroxide to iodide. When the preferred ratio of hydrogen peroxide to iodide of 1/2 is used, the preferred range for iodide anions is between 0.01 and 0.35 grams per liter in the final reconstituted formulation prior to initiation of the enzymatic reaction. However, for some contemplated applications it is possible to less effectively convert iodide into free molecular iodine and therefore the potentially useful range is for iodide is between 0.01 and 0.7 grams per liter in the final reconstituted formulation prior to initiation of the enzymatic reaction. These ranges of iodide anion in conjunction with pH and the concentration of the other additives are anticipated to yield a concentration of free molecular iodine within a range of 10 to 330 ppm.
The peroxidase enzyme of this invention is identified by the International Union of Biochemistry and the International Union of Pure and Applied Chemistry by the Enzyme Commission identification No. E.C. 188.8.131.52. Peroxidase can be obtained from a wide variety of sources. These sources include milk (lactoperoxidase) and human leukocytes (myerloperoxidase). The least expensive and most robust peroxidase suitable for this application is horseradish peroxidase. Commercially obtained peroxidase comes lyophilized as a dry powder which can then be admixed in a suitable carrier. It is anticipated that peroxidase that has been cloned from either horseradish, milk or human leukocytes will be suitable as a source of peroxidase for this application. Additionally, it has been observed that chemically modified peroxidase is suitable for use in this application. Modifications to the amino, carboxyl or carbohydrate moieties yield a suitable catalytic agent for inclusion in this application. The chemical modifications to peroxidase include cross-linking of enzyme molecules to each other, to solid surfaces or to other proteins. The chemical agents used for crosslinking include glutaraldehyde, maleimides, succinimides, carbodiimides, dicarboxylates, activated glycols, imidoesters, photoreactive azides and other agents known to one skilled in the art.
The aforementioned forms of peroxidase can be provided in a dry form such as the lyophilized peroxidase offered commercially or in a largely aqueous environment. If the peroxidase is supplied in an aqueous environment it typically will be incorporated into a medium that provides increased stability such as glycerol, dextrans, or other polyols or sugars with elevated viscosity. The peroxidase of this application can be combined with many additives whether it is supplied dry or in an aqueous environment. The concentration range that peroxidase can be used over is between 0.00005 and 0.005 mg/mL in the final composition. The preferred range is between 0.0005 and 0.01 mg/mL in the final composition.
Suitable buffering agents for inclusion in the compositions contemplated in this application include water and hydroalcoholic mixtures buffered with glycine-glycine. HCl, potassium hydrogen phthalate-phthalic acid, citric acid-Na2 HPO4, citric acid-KH2 PO4 -H3 BO3 -diethylbarbituric acid-NaOH, citric acid-sodium titrate, dimethylglutaric acid-sodium dimethylglutarate, acetic acid-sodium acetate, succinic acid-sodium succinate, potassium hydrogen phthalate-dipotassium phthalate, sodium cacodylate-cacodylic acid, sodium hydrogen maleate-disodium maleate, Na2 HPO4 -NaH2 PO4, sodium bicarbonate-5% CO2, imidazole-imidazole.HCl, boric acid-sodium borate, and the following buffers known to one skilled in the art as Good buffers Tris, MES, BIS-TRIS, AD& ACES and PIPES.
If the biological material of interest is a mammalian cell then after the free molecular iodine composition and cells are mixed sufficiently to inactivate any pathogens that may be present, the iodine composition can be separated from the cells. The separation preferentially involves washing the cells in an automated cell washer or semi-automated cell washer with an isotonic solution until the disinfectant is substantially removed. Alternatively, one can concentrate cells using a simple benchtop centrifuge, decant the supernatant that substantially contains the iodine germicide, and resuspend the cells in a medium of choice.
In a case where the sensitive biological materials consist of proteins, the iodine based germicide may be separated by precipitating our the plasma proteins in a generally conventional fashion or by passing the entire composition through a chromatographic separation medium which are very well known to one skilled in the art or by dialysis.
All of the foregoing operational principles and advantages of the present invention will be more fully appreciated upon consideration of the following detailed examples.
Example 1
Iodine based compositions were prepared that consisted principally of free molecular iodine. These compositions were prepared by incubating horseradish peroxidase, sodium iodide and hydrogen peroxide at a pH of 5.5. Allowing these materials to incubate for a defined time period and then performing an analytical characterization of the resulting formulation.
The concentration of the following three iodine species was measured: free molecular iodine, triiodide and iodide anion. Free molecular iodine was measured potentiometrically by the published method of Gottardi (Gottardi W., Fresenius Z. Anal Chem., volume 314, page 582, 1983). Two Corning model 345 pH meters were used to make the potentiometric measurements. The Corning electrodes that were used included a standard platinum electrode, a reference electrode and an iodide ion selective electrode. Triiodide was determined by performing a sodium thiosulfate titration to determine the level of total iodine and then subtracting the level of free molecular iodine from the total iodine level. The iodide ion concentration was determined directly by using a Corning iodide ion selective electrode.
There were three conditions performed in a citrate buffer at a pH of 5.5. All conditions used a final concentration of 5 ug/mL of horseradish peroxidase. The first condition (Treatment No. 1) used a concentration of sodium iodide that was 60 mg per liter and an equal molarity of hydrogen peroxide that was established using sodium percarbonate (0.025 grams per liter) as the source of hydrogen peroxide. Conditions two and three (Treatment Nos. 2 & 3) used 200 and 450 mg per liter of sodium iodide and 0.0833 and 0.187 grams per liter of sodium percarbonate respectively. The solutions were allowed to incubate for 60 minutes and the analytical measurements were made. The results were as follows:
______________________________________ppm of Iodine SpeciesI2 -Iodine I3 - -Triiodide I- -Iodide______________________________________Treatment 146.6 <2 0.126Treatment 2128 <2 0.476Treatment 3290 324 0.650______________________________________
Conclusion: It is possible to generate peroxidase based iodine germicides that are principally comprised of free molecular iodine and the ratio office molecular iodine to the other species can be controlled.
Example 2
Iodine based compositions were prepared at a pH of 7.0 that consisted principally of free molecular iodine. These compositions were prepared by incubating horseradish peroxidase, sodium iodide and hydrogen peroxide at a pH of 7.0. These components were allowed to incubate for a defined time period and then analytical determinations were performed on the resulting composition. The weights and volumes of the chemical components used were: 0.2 grams of anhydrous citric acid, weighed our, 1.3 mL of 3% hydrogen peroxide, 100 mg of sodium bicarbonate and 3 mg of horseradish peroxidase. The composition was allowed to incubate for 15 minutes prior to performing the analytical determinations described in experiment 1. The results are shown below:
______________________________________ppm of Iodine SpeciesI2 -Iodine I3 - -Triiodide I- -Iodide______________________________________178 72 0.82______________________________________
Conclusion: It is possible to generate iodine based germicides at a pH of 7.0 that are principally comprised of free molecular iodine.
Example 3
The three formulations described in experiment 1 were tested to determine their ability to inactivate Staphylococcus aureus in the presence of 106 red blood cells (RBC) per mL. The effect that these formulations had on the cellular morphology of the RBCs was determined by microscopic examination. A control experiment was performed using 10% PVP-I2 at four dilutions that ranged from 1/20 to 1/10,000.
Whole blood was processed by the Ficoll lymphocyte isolation procedure. The red blood cells (RBC) were recovered and washed once with phosphate buffered saline and held at a concentration of 3.5�109 RBC/mL in Alsevers solution at 5� C.
Agar plates were inoculated by washing the growth from Staphylococcus aureus slants with 5 mL of 0.25 molar phosphate buffer dilution water at a pH of 7.0 (PBDW) into 100 mL of PBDW. One mL of this suspension was added to each of 7 nutrient agar plates which were incubated for 18-24 hr at 35�-37�. Bacterial colonies were removed from the agar surface using 1 mL of PBDW and a sterile swab. The suspension was standardized to give about 109 cfu/mL using a Milton-Roy Spec 20 spectrophotometer.
One hundred mL of each test germicide were equilibrated to 25� C. in a water bath. Two minutes prior to the start of the assay, RBCs were added at a level of 106 RBCs/mL. S. aureus was added to a level of 2.0�108 cfu/mL. One mL samples were removed after 30, 60, 120, and 300 second exposures and neutralized in 10 mLs 0.5% thiosulfate. One mL and 0.1 mL of the neutralized samples were pour plated in quadruplicate using Plate Count Agar.
______________________________________Inactivation of Staphylococcus aureus in the Presence of Red Blood Cells Peroxidase GermicideDilution of 10% PVP-I2 Free Iodine LevelTimepoint 1:20 1:100 1:200 1:1,000 10 63 114______________________________________30 seconds pass pass fail fail fail pass pass60 seconds pass fail fail120 seconds fail fail300 seconds fail pass______________________________________
This experiment determined the ability of the disinfectant to inactivate at least 106 cfu/m of Staphylococcus aureus. The results of these measurements are shown above. All of the disinfectants were effective against the Staphylococcus aureus with the exception of 10% PVP-I2 at a 1/1,000 dilution. The peroxidase based iodine germicide exhibited a 6 log reduction of Staphylococcus aureus within 30 seconds at free molecular iodine levels of 63 and 114 ppm; at a free molecular iodine level of 13 ppm the peroxidase based iodine germicide required 5 minutes to effect a 6 log inactivation of Staphylococcus aureus.
Microscopic examination of the RBCs treated with the peroxidase based iodine germicide RBC showed no morphologic changes or reduction in counts of the cells. The RBCs treated with 10% PVP-I2 appeared discolored and shriveled with no reduction in count except for the 10% PVP-I2 sample that was diluted 1/1000. The level of free molecular iodine in the 1/1000 dilution of 10% PVP-I2 was determined to be 6 ppm.
Example 4
An enzyme based iodine germicide was generated and its ability to inactivate viruses in the presence of a high concentration of red blood cells in human serum was determined. The germicide was made by dissolving 450 mg of sodium iodide in one liter of 0.05 molar citrate buffer at pH 5.5 and then simultaneously dissolving 10 mg of horseradish peroxidase (Sigma No. P8125) and 187 mg of sodium iodide. This solution was allowed to incubate for 10 minutes and then the virucidal efficacy of this formulation was determined.
Whole blood was processed by the Ficoll lymphocyte isolation procedure. The red blood cells (RBC) were recovered and washed once with phosphate buffered saline and held at a concentration of 6.5�109 RBC/mL in Alsevers solution at 5� C.
The tissue culture ineffective dose 50 (TCID50) is the concentration of virus particles that causes 50% of a series of cultures to be infected. About 107 units of TCID50 vesicular stomatitis virus were added to samples of pooled human serum that contained 106 RBCs per mL. This sample was incubated with the germicide for 5 minutes at 4� C. and at 25� C. After five minutes the virus titter was determined as follows. In quadruplicate, the sample was serially diluted fivefold in minimal essential media (MEM) nonessential amino acids solution containing 10% fetal calf serum and 0.1% sodium thiosulfate. The dilutions were plated onto 96-well plates containing Vero cells (ATCC CRL81 ) at approximately 80% confluency. The lowest dilution used in this assay was 1:5 of the original treated serum. The cultures were incubated for 3 days at 37� C. in a 5% CO2 atmosphere and each well was examined for a cytopathic effect. At both 4� and 25� C. no cytopathic effect was observed in mock infected and iodine-treated tissue culture wells. This indicates that the germicide was effective in inactivating a lipid enveloped virus in the presence of sensitive biological material. The effect that these formulations had on the cellular morphology of the red blood cells was determined by microscopic examination. Microscopic examination of the red blood cells indicated that the peroxidase based iodine germicide did not cause a morphologic change or reduce the number of RBCs. None of the treated cells appeared discolored or shriveled. p Example 5
In the present example we demonstrate a simple, safe and effective method for killing mycoplasma in cell culture without any harmful changes in the mammalian cells. The peroxidase based iodine germicide described in this application was used in the present example to kill Mycoplasma hominis in the presence of human cells in vitro. A human cell line Hep-2 was experimentally infected with Mycoplasma hominis. Mycoplasma hominis is a typical representative of the Mycoplasma spp. genus. Mycoplasma infected and uninfected Hep-2 cells were grown in standard MEM supplemented with 5% fetal calf serum (FCS). Antibiotics were not used. Mycoplasma contamination was verified by fluorescent microscopy (stained with Hoechst dye number 33258) and by standard microbial cultivation in broth and agar.
One liter of distilled water was added to a glass beaker. Five milligrams of horseradish peroxidase was added to the beaker and dissolved. Forty two milligrams of sodium percarbonate was added to the solution and the pH was adjusted with citric acid to a final pH of 6.1. Sixty milligrams of sodium iodide was added to the solution and dissolved; this established a molar ratio of approximately 1/2 between hydrogen peroxide and iodide. This solution was allowed to incubate at room temperature for 5 minutes and then it was used as indicated below.
The peroxidase based iodine germicide at a pH of 6. was used to wash cell pellets at each passage. Cell monolayers were dispersed with saline-trypsin and concentrated by centrifugation. Cell pellets were resuspended into 10mL of the iodine germicide, mixed by vortex agitation and cells were pelleted by centrifugation. The total treatment time was 15 minutes including centrifugation.
Infected and uninfected cells were evaluated by fluorescent microscopy and microbial cultivation after each cell passage. Two surprising and unexpected results were observed. Treated Hep-2 cell morphology, growth characteristics and overall monolayer appearance were indistinguishable from untreated Hep-2 cells. One significant effect of the free molecular iodine treatment was that treated cells appeared "stained or tanned". However, the staining dissipates and treated cells are indistinguishable from untreated cells within 2 hours post treatment. Mycoplasma hominis was completely killed in all trials after either one or two treatments. When treated and untreated cells were stained and evaluated by fluorescent microscopy we occasionally observed stained mycoplasma associated with cells in iodine-treated cultures. However, mycoplasma could not be grown from treated cell cultures. A summary of the data is shown in the table below.
______________________________________ Mycoplasma Infected Uninfected Hep-2 Cells HEP-2 Cells______________________________________ Trial 1Peroxidase-iodine stain = + stain = -germicide in Hanks buffer culture negative culture negative Trial 2 stain = + stain = - culture negative culture negative Trial 1Control without germicide stain = ++++ stain = -in Hanks buffer culture positive culture negative Trail 2 stain = ++++ stain = - culture positive culture negative______________________________________ - = no stained mycoplasma cells + = 1 fluorescent spot per 20 cells ++ = 1 fluorescent spot per 1 cells +++ = 10 fluorescent spots per cell +++ = >100 spots per fluorescent cell
|Cited Patent
|Filing date
|Publication date
|Sep 13, 1960
|Apr 3, 1962
|West Laboratories Inc
|Germicidal compositions and methods for preparing the same
|Oct 16, 1978
|Oct 7, 1980
|Westinghouse Electric Corp.
|Current limiting circuit breaker with pivoting contact arm
|Sep 21, 1979
|Jun 2, 1981
|West Agro-Chemical, Inc.
|Germicidal iodine compositions with enhanced iodine stability
|Nov 9, 1981
|Jan 25, 1983
|Westvaco Corporation
|Enzymatic catalyzed biocide system
|Jun 7, 1984
|Mar 18, 1986
|Laclede Professional Products, Inc.
|Enzymatic bandages and pads
|Nov 7, 1988
|Jun 26, 1990
|Kessler Jack H
|In situ sporicidal disinfectant
|Mar 5, 1990
|Feb 26, 1991
|Kessler Jack H
|Rapid sterilization enzymatic process with persistence
|Apr 10, 1992
|Dec 8, 1992
|Kessler Jack H
|Method for simultaneously cleaning and disinfecting contact lenses
|Mar 13, 1990
|Feb 9, 1993
|University Of Southern California
|Method for disinfecting red blood cells
|F.A. Highsmith Journal of Infectious Diseases vol. 167 p. 1027 1993.
|Morrison & Schonbaum Annual Review of Biochemistry vol. 45 pp. 851 888.
|Morrison & Schonbaum Annual Review of Biochemistry vol. 45 pp. 851-888.
|Nunez & Pommier European Journal of Biochemistry vol. 7 pp. 286 293.
|Nunez & Pommier European Journal of Biochemistry vol. 7 pp. 286-293.
|Ohtaki et al. Journal of Biochemistry vol. 256 pp. 805 810 1981.
|Ohtaki et al. Journal of Biochemistry vol. 256 pp. 805-810 1981.
|Transfusion 1994 34: 322 327 entitled: Inactivation of lipid etc.
|Transfusion 1994 34: 322-327 entitled: Inactivation of lipid etc.
|Citing Patent
|Filing date
|Publication date
|Mar 24, 1997
|Dec 15, 1998
|Symbollon Corporation
|Opthalmic non-irritating iodine medicament
|Nov 12, 1997
|May 25, 1999
|Integrated Biomedical Technology Inc.
|Peroxide test strip
|Jun 2, 1998
|Jul 11, 2000
|Integrated Biomedical Technology, Inc.
|Peroxide and chlorine test strip
|Dec 12, 2000
|Aug 13, 2002
|Symbollon Corporation
|Non-staining topical iodine composition and method
|Jun 2, 2000
|Jan 4, 2005
|Oxibio, Inc.
|Methods and devices for providing anti-infective activity to a medical device
|Jun 18, 1999
|Sep 6, 2005
|Oxibio, Inc.
|Medical device having anti-infective and contraceptive properties
|Apr 23, 2003
|Jun 8, 2010
|Insense Limited
|Wound dressings comprising hydrated hydrogels and enzymes
|Jan 11, 2011
|BioLargo Life Technologies, Inc
|Material having antimicrobial activity when wet
|Jun 4, 2004
|Apr 19, 2011
|Archimed Llp
|Skin dressings containing oxidoreductase enzyme
|May 17, 2011
|BioLargo Life Technologies, Inc
|Absorbent systems providing antimicrobial activity
|Sep 20, 2011
|Biolargo Life Technologies, Inc.
|Systems providing antimicrobial activity to an environment
|Jul 24, 2012
|Biolargo Life Technologies, Inc.
|Systems and methods for cleaning liquid carriers related applications data
|Sep 4, 2012
|Biolargo Life Technologies, Incorporated
|Systems providing at least pesticidal activity
|Aug 10, 2001
|Feb 13, 2003
|Wilson Burgess
|Methods for sterilizing biological materials using dipeptide stabilizers
|Aug 20, 2004
|Apr 7, 2005
|Larsen Robert K.
|Dental appliance cleaners and methods for making and using them
|Jan 28, 2005
|Aug 18, 2005
|Insense Limited
|Skin dressings
|Apr 23, 2003
|Feb 16, 2006
|Davis Paul J
|Wound dressings comprising hydrated hydrogels and enzymes
|Apr 7, 2006
|Aug 17, 2006
|Clearant Inc.
|Methods for sterilizing biological materials using dipeptide stabilizers
|Sep 7, 2006
|Mar 13, 2008
|Biolargo Life Technologies, Incorporated
|Ground treatment for reducing microbial content
|Sep 7, 2006
|Mar 13, 2008
|Biolargo Life Technologies, Incorporated
|Material having antimicrobial activity when wet
|Oct 11, 2007
|Apr 24, 2008
|Biolargo Life Technologies, Inc.
|Systems providing antimicrobial activity to an environment
|Jan 18, 2008
|May 29, 2008
|Biolargo Life Technologies, Incorporated
|Systems and methods for cleaning liquid carriers related applications data
|Dec 7, 2007
|Jun 19, 2008
|Biolargo Life Technologies, Incorporated
|Absorbent systems providing antimicrobial activity
|Jan 31, 2008
|Aug 14, 2008
|Biolargo Life Technologies, Incorporated
|Systems providing at least pesticidal activity
|Apr 12, 2007
|Oct 16, 2008
|Natasha Aksenova
|Method for the treatment of nail disease
|Jul 24, 2008
|Jan 29, 2009
|Biolargo Life Technologies, Incorporated
|Material having antimicrobial activity when wet
|Mar 10, 2006
|Mar 26, 2009
|Jan Jezek
|Skin Dressings
|Dec 19, 2008
|Apr 23, 2009
|Natasha Aksenova
|Method for the treatment of nail disease
|Jul 18, 2007
|Jul 2, 2009
|Paul James Davis
|Hydrogen Peroxide Delivery System
|Jul 22, 2010
|Paul James Davis
|Wound Dressings comprising hydrated hydrogels and enzymes
|Jun 2, 2000
|Aug 1, 2007
|Oxibio, Inc.
|Anti-infective medical device and production method
|May 31, 1997
|Oct 1, 1998
|Symbollon Corporation
|Opthalmic non-irritating iodine medicament and method
|Jun 2, 2000
|Dec 14, 2000
|Oxibio, Inc.
|Anti-infective medical device and production method
|Dec 28, 1999
|Jul 5, 2001
|Symbollon Corporation
|Non-staining topical iodine composition
|U.S. Classification
|424/94.4, 435/188, 424/667, 514/2.3
|International Classification
|Cooperative Classification
|European Classification
|Jan 12, 2001
|Fee payment
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Rauschenberg Foundation Names 2016 Artist as Activist Fellows
Chapter NY to Upgrade to Bigger Space on East Houston Street
Strong Results at Sotheby's Contemporary Evening Sale Allay Post-"Brexit" Fears
Arrest Made for Theft of $250,000 Worth of Art from Trailer in LA
Getty Research Institute Names Fiona Tan 2016–2017 Artist in Residence
Architect Michael Graves's Residence and Studio Sold to Kean University
Australian Arts Community Protests $224 Million Cut to Federal Arts Budget
"Mabel Dodge Luhan & Company: American Moderns and the West"
"Mabel Dodge Luhan & Company: American Moderns and the West"
"Mabel Dodge Luhan & Company: American Moderns and the West"
Travis Diehl on Los Angeles Poverty Department's What Fuels Development
Claudia La Rocco on Cecil Taylor, Miami City Ballet, and Vicky Shick
Jennifer Krasinski on Brodsky/Baryshnikov and Krapp's Last Tape
Claudia La Rocco on Trajal Harrell's The Ghost of Montpellier Meets the Samurai
Claudia La Rocco on Maria Hassabi, Pam Tanowitz, and Justin Peck
Oskar Fischinger, Raumlichtkunst, 1926/2012. Three-screen projection comprising three 35-mm films transferred to HD video, black-and-white and color. © Center for Visual Music. Installation view, Whitney Museum of American Art, 2012.
IN TATE MODERN'S recently opened Structure and Clarity Collections, a passage off to the side of the light, expansive gallery space leads to a large, pitch-black room. Here, visitors can immerse themselves in Oskar Fischinger's Raumlichtkunst, ca. 1926/2012, a film performance recently acquired by the Tate. The latest iteration is a re-creation produced by the Center for Visual Music in Los Angeles; the work comprises three ten-minute-long reels that are variably looped to make ever-new combinations. The Center took original nitrate and transferred it to 35-mm film and then to high-definition video, digitally restoring the color in the process, and also added a new percussive accompaniment—a track by Edgard Varèse and another by John Cage and Lou Harrison—derived from details of the original film events.
While all three projections offer evidence of a variety of methods, each screen is nonetheless dominated by a single technique familiar from Fischinger's "visual music" animations. On the left, orchestrated groups of long rectangular forms push upward and sideways in ripples and forceful, angular thrusts. The right screen demonstrates his wax slicing technique (Wax Experiments, 1921–26), which uses methods redolent of those used to make murrine and millefiori glass to create liquid swirls. The subsequent vertigo effect draws viewers into a colored maelstrom, which, when it reverses, suggests infinite cosmic space. The center screen uses a combination of the other two and adds fluid graphic forms and celestial depth.
Fischinger's creative philosophy was bent toward generating emotion through non-naturalistic, abstract, "absolute" form, color, and music. He was a filmmaker who also painted, and with Raumlichtkunst (a compound German word that translates as "space-light-art") the variety of his experimentation can be experienced in one work. While Fischinger is still best known for his "visual music," this three-ring circus of an installation is an occasion to revisit the given histories of expanded animation. REWIND, an important British genealogical project researching and archiving electronic media arts, includes Fischinger's Bauhaus contemporaries (Theo van Doesburg, László Moholy-Nagy) in its history of expanded cinema, but makes no mention of Fischinger. This latest iteration of Raumlichtkunst offers undeniable evidence that he was an early pioneer of the canon.
Oskar Fischinger's Raumlichtkunst is on display through October 28 at the Whitney Museum of American Art in New York and through May 12, 2013, at Tate Modern in London.


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Shaynna Blaze, Selling Houses Australia's Interior Designer, from The LifeStyle Channel on Foxtel gives her tips on how to plan the hugely popular ensuite bathroom.
Shaynna's top tips on ensuites
- People expect toilet, basin and shower – anything less is a powder room
- Utilise the dead space under the sink for shelving and storage
- Add a touch of indulgence – an overhead range shower head perhaps.
Watch more:
- Figuring out the style and function of your space
- Bathroom fix up tips for sellers
- Andrew Winter: How to climb the property ladder
Read more:
- 10 incredible bathrooms you won't believe exist
- What is a wet room?
- Planning essentials: Trends in bathroom design
Selling Houses Australia can be seen Wednesdays at 8.30pm on Foxtel's The LifeStyle Channel from February 3.
Follow us on Twitter for more news, tips and inspiration. Become our mate on Facebook and explore our Pinterest boards.
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Federer and Fish, a Wilson BLX Final in Cincinnati
Roger Federer and Mardy Fish will go head to head with their Wilson Six.One BLX rackets for the Western & Southern Financial Group Masters title in Cincinnati.
"Wilson is proud to have both finalists playing with the new BLX technology," said Wilson Global Tour Director Massimo Calvelli. "The tennis has been terrific this week and it's nice to see Roger and Mardy find their form leading into the Open."
Roger Federer eased into the finals with a 6-4 6-3 win over Baghdatis. Defending champion, Federer is vying for his fourth Cincinnati title.
Mardy Fish has been in outstanding form throughout the summer hard court season, taking the singles title in Atlanta and doubles title in Washington DC. This week, he defeated ATP Tour World #8 Verdasco, #4 Murray and former World #1 Roddick en route to the final and put himself in position to capture his first ATP Tour Masters Series tournament title.
The Western & Southern Financial Group Masters Series finals will be played at 2pm Eastern time.
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Quinton Jackson says people should 'lighten up' over gay slurs
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Reader comments
What an idiot. He protests that he's not homophobic then goes on to make lots of ignorant statements in his defence. Clearly not the brightest person around.
It's all fun, just a laugh, we should bring back Love Thy Neighbour, they were just having a laugh around black people, or send him a Robertson's Golliwog as a humorous jibe.
"Like most straight guys I joke around with the whole gay thing and I see it as comedy, not saying that's right or wrong but I don't do it out of hate"."
Like most white guys I joke around with the whole black think and I see it as comedy, not saying that's right or wrong but I don't do it out of hate.
I wonder how Mr Jackson would react to that?
So if I joked around about skin colour and slavery around Quinton Jackson he would not get offended right! So what the difference between the two types bigoted behaviour that one is aright in Quinton's eyes?
It also sounds like Quinton Jackson has some issues about his own sexuality if he thinks all men are that interested in him!
Posing topless with a fetish style bike chain round your thick steroid enhanced neck is more than "kinda gay"
"Not only did I enjoy myself but I was relieved that the guys at this bar did not hound me once," he wrote."
Probably because they saw how much of a nasty piece of work you are
I for one dont take offence, again I see the funny side but those who dont make life a lot harder for themselves. If had explicitly been homophobic I would have taken issue but to me it rolls of my shoulders. Again Peter Tatachell lovers protest….sigh
so I guess his Ok being called teh N word plus many others then? Just liken up dude
no 7: Chutneybear: "I for one dont take offence, again I see the funny side"
So long as Quinton Jackson sees the funny side of being called a n****r then I agree with you.
Whatever Quinton, I'm still not going to see your sh*tty film.
Well if acting makes you kinda gay and soft – why is this big black softy seeking a career in the big old gay film business? derrrr Dumb '……' – see I'm evolved enough to know when to stop – evolution makes you kinda Tolerant – Hey Queertin try evolving!
Well you would have to ask him, I dont get hot under the collar… while you are at it why not prosecute Frank Boyle,Jimmy Carr while you are at it..
He shoud lighten up when ppl use being black as an insult, fuc'king nig'ger
I agree with posts 1-6. People can 'lighten up' when they feel equally comfortable with the same statements/generalisations made with the word 'black' or 'Jew' substituted for 'gay'.
Chutney bear, what PinkNews isn't reporting but was WIDELY reported here in the US was that Jackson used the word "faggot" numerous times during the interview, even calling a fellow cast member a "faggot" for making fun of him.
I would probably not have taken much offense if he had only done what PinkNews is reporting but I do take offense when he uses a word that is equivalent to the "N" word and then trying to justify it as, "just having fun".
By the way, HE doesn't get to decide what's acceptable and what is not when it comes to what is offensive to gay people. HE doesn't get to decide if his use of "faggot" is OK or not, just as I don't get to decide what should and should not offend black people or whether or not it's OK to use the word "nigga" without it being an offensive slur.
the only thing i'm surprised at is a neanderthal like him knowing how to write a blog.
Same could be said about slavery. In fact shouldn't the balck community be thankful?
I for one dont take offence, again I see the funny side but those who dont make life a lot harder for themselves. If had explicitly been homophobic I would have taken issue but to me it rolls of my shoulders. Again Peter Tatachell lovers protest….sigh
I love comments like this. Prey do tell when should a line be drawn against homophobia? Ignore the first comment, turn your back on a series of verbal abuse, take the first punch on the chin. Lick the first stab wound? When would you advise that I take this as playful banter and when should I get concerned and worried. Any behavior like this is a rolling ball . It starts with words and then leads to other actions . Then sometimes death. My mate was called a faggot, they then proceeded to cave his head in with a hammer. I wonder when he thought, oh this is just harmless banter and when he changed his mind too, I am going to die!
No 18: Dave: you say: "I for one dont take offence, again I see the funny side but those who dont make life a lot harder for themselves."
Fair enough.
Just so long as you would expect black people to see the funny side of being called n****r in the same lighthearted manner.
Otherwise you're just defending a homophobic double standard.
MartinM, dave was commenting on ChutneyBear's comment. His first paragraph was not his own. Maybe read the whole way through a comment before lambasting someone for it?
I wasnt aware he used the word faggot now that is crossing the line. BTW I take it none of you here like Frankie Boyle or Jimmy Carr?
Quinton Jackson has only one oar in the water. It happens.
These comments are un-moderated and do not necessarily represent the views of PinkNews. If you believe that a comment is inappropriate or libellous, please contact us.
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The Best Grilled Pompano You'll Ever Have
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Book Offers Taste of The Dock's 40 Years
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The Shanks
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Eligible: A Modern Retelling of Pride and Prejudice
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How they match up: Ducks-Predators
DUCKS (6-7-1) vs. NASHVILLE (5-3-3*)
Time: 5 p.m.
Site: Honda Center
TV/radio: Prime Ticket; KLAA/830
How they match up
|How they match up
|Goals per game
|Goals-against avg.
|Power play
|Penalty killing
*Record and statistics through Friday
Nashville has struggled mightily on the road against the Ducks with a 2-17-3 record all-time at Honda Center ... The Predators did prevail here last season and they handed the Ducks a 4-1 loss in Nashville on Oct.9 ... Nashville earned points in its first eight games (5-0-3) but had dropped three straight entering Saturday's game against the Kings ... D Ryan Suter (lower body injury) is out while RW Martin Erat (back) and C Matthew Lombardi (concussion) are day-to-day.
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Enjoy a different look at the work of the great landscape designer.
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Our next market takes place 25th and 26th June. Free admission.
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