text:
Cubs' Non-Baseball Business is Booming
"...Elton John's going to help us win some ballgames."
So says Crane Kenney in explaining how the extra revenue the Cubs will realize from three Wrigley Field concerts this summer, including the Elton John/Billy Joel event in late July, will translate into additional payroll flexibility.
More from Kenney:
"The CBOE [seat] auction last year paid for Rich Harden. The 'Road to Wrigley' game sponsored our Asian scouting operation. That's the way, from the business end, we look at these things. All
these elements really help our business move forward. My view is if
you're a Cub fan, you should enjoy the concerts whether you're an Elton
John fan or not."
The Trib's Dave van Dyck also reports that the Cubs will be opening a new, year-round, 7,000-square-foot sports bar at Addison & Sheffield in time for the home opener on April 13th. The Harry Caray's Restaurant group will run the place, which will replace the Friendly Confines Cafe and be accessible to fans whether or not they hold a ticket to the game.
"We need to compete with all the other bars and restaurants in town,"
Kenney said.
Indeed. We can't have our baseball team not competing with all of the city's bars and restaurants.
Re: Cubs' Non-Baseball Business is Booming
im not letting someone who tells me to enjoy an elton john concert tell me what to do.
Re: Cubs' Non-Baseball Business is Booming
It's getting late have you seen my mates
Ma tell me when the boys get here
It's seven o'clock and I want to rock
Want to get a belly full of beer
My old man's drunker than a barrel full of monkeys
And my old lady she don't care
My sister looks cute in her braces and boots
A handful of grease in her hair
Don't give us none of your aggravation
We had it with your discipline
Saturday night's alright for fighting
Get a little action in
Get about as oiled as a diesel train
Gonna set this dance alight
`Cause Saturday night's the night I like
Saturday night's alright alright alright
Well they're packed pretty tight in here tonight
I'm looking for a dolly who'll see me right
I may use a little muscle to get what I need
I may sink a little drink and shout out "She's with me!"
A couple of the sound that I really like
Are the sounds of a switchblade and a motorbike
I'm a juvenile product of the working class
Whose best friend floats in the bottom of a glass
Re: Cubs' Non-Baseball Business is Booming
i dislike you with great intensity.
Re: Cubs' Non-Baseball Business is Booming
"...Elton John's going to help us win some ballgames."
Are they continuously playing his 80's music into the visitor's club house?
Re: Cubs' Non-Baseball Business is Booming
Dunno, but I bet he gives a pretty good reverse chalupa.
Re: Cubs' Non-Baseball Business is Booming
The WBC play by play guy called an out to Granderson "a line out to central"
Re: Cubs' Non-Baseball Business is Booming
US loses 10-6. Guthrie gave up 6 in the 2nd inning. US was able to cut it to 7-6, but then LaTroy gave up a 3 run bomb. Sounds about right.
Still haven't figured out who the US will play in the semis. They still have to sort out the seeding between the Koreans & Japan/Cuba.
Re: Cubs' Non-Baseball Business is Booming
"The desperation defensive deployment backfired Wednesday night in the World Baseball Classic when Dunn made a wild throw that led to four unearned runs and Venezuela won Group 2 by beating the Americans 10-6 to conclude second-round play in Miami."
the best part...he was playing 1st and it was a throwing error
adam dunn...you go girl!
Re: Cubs' Non-Baseball Business is Booming
It would have made more sense to play DeRosa at first and Dunn in left.
Re: Cubs' Non-Baseball Business is Booming
Check it out. The Indians don't want DeRosa playing first in the WBC. That put Dunn there.
Re: Cubs' Non-Baseball Business is Booming
Check it out. The Nationals didn't want Dunn playing first in the WBC.
Re: Cubs' Non-Baseball Business is Booming
and I quote Johnson
"Adam Dunn is playing first base tonight, out of necessity, but the Nationals don't want him there in the next round. Mark DeRosa can play first, but the Indians don't want him playing there, Johnson said."
tickets again
Thanks for the replies in the previous thread about tickets - i will try and upgrade my tickets from ebay or stubhub. one quickie, if the tickets are to be printed at home, and they forward the email onto me, what is to stop them selling the seats again and forwarding on the same email to someone else???
sorry never used stubhub, and print at home tickets have only just caught on over here - i especially like it when they charge the same amount as if they have to post to you...
Re: tickets again
"forward the email onto me, what is to stop them selling the seats again and forwarding on the same email to someone else???"
You just have to rely on their honesty. If you get to the game and they don't work ebay has a process to get a refund (but that's a pain in the ass). That's why I always go with hard tickets. I don't mess with the print at home tickets.
Stubhub I believe only sends hard tickets, and their prices aren't too bad. You can just get more of a deal on ebay I think if you catch the right auction. But I'd only bid on auctions that have tickets in hand, or are hard tickets.
Re: tickets again
Since Stubhub is affiliated with MLB, they have an eDelivery option which allows the seller to enter the bar code of the tickets. Stubhub then contacts the Cubs which invalidates that bar code and issues tickets with new bar codes to you. At least this is how I understand how it works.
This wouldn't apply to eBay.
Re: tickets again
thanks for that, will go with stubhub then
Re: tickets again
Yeah, I wouldn't buy print at home tickets on ebay. Stubhub is expensive, but really reliable.
Re: Cubs' Non-Baseball Business is Booming
So it sounds like from this latest Cubs.com article that Marshall has been official given the starting spot over Heilman, and that Samardzija will take Lilly's spot in the spring rotation until he gets back because Lou wants to get him more experience.
Re: Cubs' Non-Baseball Business is Booming
I'm sure Elton John is hitting the Target Demographic of Wrigleyville.
Just noticed: The Houston Astros are emphasizing speed and "manufacturing" runs this spring. They lead the major leagues in triples with 11 and have stolen 18 bases while being caught only once.
They also have a total of (1) win which must be some kind of record.
Re: sCRAPPY
Manufacturing in this country is not in a good position right now. Neither are the Astros.
Re: Cubs' Non-Baseball Business is Booming
The story is funny, but the photo is pretty creepy.
Re: Cubs' Non-Baseball Business is Booming
I wonder if this new sports bar is going to charge $6 for an Old Style so you really feel like you're at the game?
Also, I kind of like their Fund Accounting approach. I'd like my bar tabs at this new Wrigley Field sports bar to go towards a top flight free agent starting pitcher next year.
Re: Cubs' Non-Baseball Business is Booming
I paid $5.95 for a can of Old Style once. I was at a bar in Evansville, IN and I saw it in the cooler. Being from Ohio I've never even seen an Old Style, except I knew it was the beer of Wrigleyville. Thinking, "it's a can of beer, what's it gonna be, 3 dollars max?" Bill comes, $5.95 for a can of Old Style. The bartender said it was their special import of the month. Or something stupid.
Re: Cubs' Non-Baseball Business is Booming
The Trib web shows the sod (from co.) being replaced at Wrigley. Is that to get it ready for the concerts?
Re: Cubs' Non-Baseball Business is Booming
So is that picture some new fund drive - "We leave Elton up until we receive 100 pledges?" Please, make it go away!
Re: Cubs' Non-Baseball Business is Booming
What's with all the Elton John hate? I am going to go sing some tonight and dedicate it to you guys.
Recent comments
Not sure I agree with this logic. The Cubs are one of the best teams in baseball, they have spent heavily on the team over the past couple of seasons, have more hitting prospects than they have places for them to play (and more on the way), a clear weakness in the bullpen, and have a 1.5 year window with Arrieta (to say nothing of the likely declines of Lester and Lackey).
Unless you think (maybe even if you do think) Schwarber is the next coming of Babe Ruth, I would certainly consider trading him if it brought back a couple of the best relief pitchers in baseball.
Miggy was going to sit anyway with Lester starting.
In related news, do any of the Cubs pitchers want to throw to Contreras?
Only Russell and Miggy failed to reach base yesterday. Both sit today.
Trading Schwarber actually makes a lot of sense because his actual position is currently being taken by Anthony Rizzo, unless they vote in the DH this offseason.
Trading him for a reliever is never going to happen though. I don't think you can trade Schwarber unless you get a CF'er for a few years (presume Fowler finds his multi-year deal next offseson) or a couple of high end young starters...or an established starter...a good one like Chris Sale.
I don't see what the Yanks have that the Cubs need.
peter gammons on MLB network pushing the yanks/schwarber angle...says insiders from the yanks say brian cashman highly covets him.
unless that conversation starts with a.miller (2/18m left on contract) i dunno how you even start that conversation given the "not trash, but no stars" state of the yanks minor league system.
they got corner OF'r aaron judge, C gary sanchez, RHP james kaprielian's questionable elbow, RHP domingo acevedo...all interesting, none expected to impact the game like schwarber's power potential.
Zo, Heyward, KB, Riz, Contreras (LF), Baez (SS), Szczur (CF), Ross, Lester
damn. low sample size blah blah whatever, but that slash got sneaky good. 10 hits, 4 walks, 2hr, 1 double through 39 PA...nice
Buddy Ryan takes his place alongside George Allen as the great Bear head coaches who never were.
Well, at least he can still hit: Jake slashing .294/.368/.868. Forget hitting 8th -- he should hit 6th.
Curious to see today's lineup vs. a LHP: Ross needs to catch Lester, Joe likes Javy at 3B when Lester pitches. Heyward with back-to-back good games at the plate, and is actually hitting a little better against LHP (.247/.341) than RHP (.232/.321), although neither is much to write home about. But, tomorrow is an early day game, so some regulars will probably sit either today or tomorrow.
Maybe Willson in LF, KB in RF and Almora in CF? If so, Albert Jr., assume every ball is yours.
"Pitching prospects not looking so hot"
That reminds me that Lucas Giolito will start for the Nats tonight.
Well, there you go. Federowicz is one of the best at balls in the dirt.
Reds pitching meeting tomorrow:
"Hey coach...maybe we should walk Bryant?"
"No...keep challenging him with fastballs! What kind of man are you?! The Cubs walked Harper, and look what happened there! They got teased for it, by one of the Nat players! Is that what you want?"
How can I get that feature? I think it woudl be a win-win for all involved.
Ian Happ is hitting.733 after 5 games at Tennessee. He went into tonight's game hitting .667 -- and went 3-for-3 to raise his average.
2-for-3, including a bomb, for LaStella at Iowa. Pierce Johnson very bad -- 2IP, 5ER. Pitching prospects not looking so hot -- Underwood has been awful.
Did Jiminez get hurt? I noticed he came out early yesterday.


text:
Disabled children's ministry brings together generations of Korean-Americans
Congregation seeks to overcome cultural prejudices against handicapped children
Every child receives a 'musical goodbye' at the close of the service at Klema (Photo: OnFaith)
July 16, 2014
The sea of hipster V-neck shirts and gray suits split from one another as Korean hymns and American praise tunes call out to each from opposing buildings. Rows of snappy Millennials fill the English-speaking sanctuary. A smattering of young couples and singles stand in unison, the occasional hand reaching up as an electric guitar prompts lyrics like, "I've finally found where I belong."
The pastor, wearing loose-fitting jeans and an un-tucked shirt, preaches on shame and the desire to shed labels. California charm laces his charisma. It's a congregation of Korean-American young professionals, well heeled and confident.
Across a sheet of AstroTurf, the mother church is situated somewhere between Seoul and a Maranatha choir. Most of the 800 Korean speakers in this congregation are blue collar and middle management types, ages 50 and up, who rise and fall obediently to the pastor's cues. Bibles are opened for underlining. Prayer is conducted out loud and in unison, a divine trust lacing what otherwise feels like the air of stable, seasoned parents.
These two congregations, one English, one Korean, form Open Door Presbyterian Church, the second largest Korean-American congregation in Fairfax, Virginia.
Open Door is located in the most highly educated, affluent pocket of Washington, D.C.'s metropolitan outskirts. Asian Americans have come here in droves, now making up the area's largest minority group at 18 percent. There are currently 41,356 Korean Americans settled in Fairfax, putting the Washington metro area just behind Los Angeles and New York as home to America's largest Korean communities.
The bulk of this migration to Virginia comes from the second generation, returning from college and entry-level jobs elsewhere. As they come home, they create a wave of unfamiliar dynamics as the aging generation, which emigrated after the Korean War, now encounters its ascending (and Americanized) sons and daughters. Open Door, a church pioneering the bilingual model, presents a microcosm of these shifts.
An unexpected linchpin
In 2003, one member of the English-speaking congregation was itching to leave. Christine Pak felt the church was established to the point that her capacity to serve was diminished. She was literally walking out when the Korean-speaking Pastor Paul stopped her in the hallway.
"Why are you leaving Open Door?" he asked.
"Pastor Paul, it's time," Pak said. "I need to do what I'm called to do."
Pastor Paul asked what her calling was.
"I'm a Special Ed teacher," she said. "I'm supposed to be with children with disabilities."
Pak is a cultural anomaly among Korean-Americans — she sees the disabled as of no lesser value. Historically, cultural prejudices against abnormality have meant that Korean-Americans with handicapped kids don't expose them to the public eye, particularly within their own communities.
"What?" the pastor said to Pak. "I can't believe you are a Special Ed teacher! We have families that have disabled kids on our Korean-speaking side. I've never known what to do with them. Moms and dads just wander around with these kids while the others leave them at home."
And so Pak stayed, beginning the long and arduous trek that resulted in the creation of Klema, a ministry specifically geared toward serving those with autism, Down syndrome, and other cognitive and developmental disabilities. It was a lonely road, especially in the context of a church where immigrant congregants are trained to attain worldly success. There was skepticism about investing in those who had no such future.
"They thought we were just doing something 'nice' for these kids," Pak said. "Like we were providing babysitting services. Very few understood that this was about respecting these kids as whole persons, about providing a place for them to worship as fully and freely as the rest of us."
Pak's husband, Thomas, joined her in building Klema's theological foundations after graduating from Fuller Theological Seminary in California. "These kids deserve to worship. So many in our Korean-speaking congregation would tell us, 'What are you doing? The kids can't understand the songs or the sermon.' But it's not about that! It's about souls communing with God. You cannot deny any person the right to worship God," Thomas said.
A typical Sunday morning at Klema under the Paks' watch is highly structured. The children arrive and are greeted by volunteers or "buddies," who pair up one-by-one and guide them through the morning's activities. Two circles of chairs are laid out for the welcome to worship, the disabled students on the inner ring, their buddies (ranging from ages 14 to 67) sitting just behind them. Both lead the singing — the teenager with Down syndrome playing the drums and a preppy volunteer from Thomas Jefferson High School setting the pitch. After worship there's a sermon, then Bible study groups split up by cognitive ability, followed by playtime and a Korean buffet. Illustrations of the routine are plastered on the walls.
"The focus was all about the kids," Pak said. "I only accepted volunteers who understood this. No cliquishness was allowed. This wasn't about high-achievers feeling good about their own service. It was about serving the kids and being served by them, worshipping together as a community of equals."
The approach worked. Within months of Klema's founding, the ministry swelled to 30-40 kids, each matched by one to two volunteers so the room filled to capacity.
Full Story: Let the Weak Say I Am Strong
Source: FaithStreet
Marriage: Making sense of Nepal's complex culture
'It might sound unusual to talk about caste as being part of the church but it is the truth of our context'
Party watchdog to conduct more inspections of state bodies
Chinese security agency created to oversee the persecution of Falun Gong group is among those to be inspected
Marian devotees challenged to be missionaries
Filipinos mark 150 years since Redemptorist priests became custodians of Our Lady of Perpetual Succour icon
Government instructs against using 'Rohingya' or 'Bengalis'
Myanmar's new term 'Muslims in Rakhine State' is debated and seen as controversial
Religious groups step up liquor ban demand in Kashmir
State government rejects call for prohibition saying consumption is a 'matter of choice'

subset: commoncrawl


text:
Login | Sign Up!
Quick fixing and decorating bag that is damaged, with lace and bow to make it kawaii, with this DIY tutorial! For more details visit: ... This quick fix diy is a video where I try to fix my fashion item that is damaged. Today's video i
by Actually Ellen
by HelenMay Crochet
by mahumtariq
by WillCFish Fishing TipsandTricks
by MandyJeanChic
by Harleigh Snyder


text:
List all words ending with journalese
Only the word journalese was found, nothing longer.
Some random words: vetiver elain llama hm jotty ivermectin twyer
This is not a dictionary, it's a word game wordfinder.
- Help and FAQ - Examples - Home
Privacy and Cookies Policy - Share - © Copyright 2004-2016 Morewords.com - 62.515mS


text:
Relevant offers
Filming has begun on a movie drama about Julian Assange and his WikiLeaks website that director Bill Condon said will not attempt to pass final judgment on the former hacker and his influence.
Hollywood movie studio DreamWorks said on Wednesday that The Fifth Estate, starring British actor Benedict Cumberbatch as Assange, will open in US movie theaters in November 2013.
The movie will trace the early days of WikiLeaks through the eyes of Assange and his German former spokesman, Daniel Domscheit-Berg (played by Daniel Brühl), culminating in the release in 2010 of a series of thousands of damaging government documents and emails.
"It may be decades before we understand the full impact of WikiLeaks and how it's revolutionized the spread of information. So this film won't claim any long view authority on its subject, or attempt any final judgment," said Condon in a statement.
"We want to explore the complexities and challenges of transparency in the information age and, we hope, enliven and enrich the conversations WikiLeaks has already provoked," added the director, whose past projects include musical "Dreamgirls" and "The Twilight Saga: Breaking Dawn."
The movie screenplay is based on Domscheit-Berg's 2011 book Inside WikiLeaks, written after the disillusioned German activist quit the organization, and a second book, WikiLeaks: Inside Julian Assange's War on Secrecy, by journalists David Leigh and Luke Harding.
Assange, who grew up in Australia, was also profiled in the 2012 Australian TV film Underground.
The WikiLeaks founder has been holed up in the Ecuadorian embassy in London for seven months to avoid extradition to Sweden for questioning on allegations of sexual assault.
Assange has said he fears extradition will ultimately lead to him being sent to the United States, which is furious that WikiLeaks has leaked hundreds of thousands of secret US diplomatic and military cables.
- Reuters
Sponsored links
Chinese website forced to shut down 'insurance policy' for Taylor Swift's love life
Tropfest rescued with help from George Miller and major sponsor CGU
House from The Castle faces demolition
Carolyn Robinson recruited by Television New Zealand
Aaradhna: 'I've been called curry muncher, dumb coconut'
Longer runway could help Wellington land more big concerts
Backstage at DANCE on grove's Beauty and the Beast
Beauty and the Beach: Artificial nipples and painful viewing?
Broods' Conscious summer pop with an edge
Emma Bunton reassures fans that Spice Girls reunion will happen
New trial for Adnan Syed, convicted murderer in podcast Serial
Iggy Azalea broke up with Nick Young after catching infidelity on 'security footage'
Simon Sweetman: Heroes live - Neil Young
Victim of sexual assault describes how she lashed out at assailant
Caleb Kovaleski hands himself in after Palmerston North manhunt
Chiefs keep Super Rugby title hopes in good shape after beating Crusaders in Fiji
Court bid accuses caregiver of grooming Akaroa client to leave her property
Hit by a car, run over by a train, trapped in a van
Second car plunges into Wellington Harbour at Lowry Bay in one week
The Battle of the Somme lasted five months and cost more than one million lives
Marlborough transgender student's bathroom battle brings about change
Inability to respect soggy Suva conditions could cost Crusaders dearly
Recap: Brisbane Broncos v Melbourne Storm - NRL Round 17
Recap: ACT Brumbies v Queensland Reds - Super Rugby Round 15
Chiefs keep Super Rugby title hopes in good shape after beating Crusaders in Fiji
Australian federal election 2016: Count looms as one of the most engrossing in living memory
Sir Graham Henry joins Leinster coaching staff
Carolyn Robinson recruited by Television New Zealand
Chinese website forced to shut down 'insurance policy' for Taylor Swift's love life
TVNZ Breakfast: Rawdon Christie comes to Melissa Stokes' rescue as camera zooms in on split skirt
Longer runway could help Wellington land more big concerts
Orange is the New Black star Danielle Brooks congratulated on first class ticket
Kitty Genovese's murder became the stuff of legend - but everyone got the story wrong
Giant glass mural in Wellington's CBD to come down piece by piece
House from The Castle faces demolition
Quiz: How well did you watch Game of Thrones, season 6?


text:
Keep Fabric Smelling Fresh
file size 0.941244 mb
1/14/2011 12:00:00 AM - time 0:58
Fabric is a sponge for odors. In spaces with upholstered surfaces, give tenants and visitors a great first impression by making sure furniture doesn't smell like smoke, body odor, food, must or other offensive odor. After cleaning, janitors can use an odor control spray designed for fabric and upholstery.
In commercial buildings, these include office chairs, cube partitions and lobby furniture. For those cleaning hotels, each room's furniture, bed lines, pillows and fabric shower curtains should be addressed along with the lobby furniture.
In healthcare facilities, focus on guest chairs in patient rooms as well as furniture in waiting areas. Finally, in schools, upholstered furniture in teacher and student lounges and libraries are great targets.
Choosing The Right Mop Handle
Tools That Benefit Front-Line Custodians
Comparing Two Service Providers
A Tale of Two Custodians
Healthy Custodians Are More Productive
Clarifying Customer Expectations
Tools That Keep Staff On Schedule
Cleaning Products That Improve Productivity
Tips To Cleaning Corroded Urinals And Toilets
Tools That Improve Productivity


text:
Scout Capital, who spoke at Grant's conference in the past, and who has talked so nicely about BAM, have cut their holdings by over a million shares in this reporting quarter.Third Ave reports today.Kaminsky team were net purchasers over the quarter. And if I am not mistaken, so was Murray Stahl, I think he is Horizon assets.Pass the Kool Aid please, made from fresh NOI and plenty of free cash flow (as long as you dont include interest expense and capex in the recipe).
>>Third Ave reports today.<<They reported today, and dropped 2% of their position. Some will argue just reallocation after great gains, others might debate that their research is becoming more refined for BAM.
Once again, an interesting question poses itself.Exactly which long term institutional holders were buying during the swan dive in share price in the belief BAM was a compelling buy this summer?Huffing, puffing and name calling aside, show me the money!


text:
Latest update June 27th, 2016 12:55 AM
Dear Editor,
Reading the Letters section of the newspaper recently, I came across a letter written by a Paul Seecharran (A defining and captivating moment) about Leader of the Opposition, Robert Corbin's character. I don't know where this writer was or is living all the time but it was really amazing to see that he could judge a man after attending one funeral and making allegation about people planning to disturb a highly publicized funeral of a TRUE GUYANESE hero.
Perhaps that writer might have other intentions but, I would be grateful to hear what he had to say when the late Winston Shripaul Murray was verbally abused in Congress Place on the Election Day when he contested the leadership of the PNCR.
That same goodly gentleman that the writer is praising was present when some hooligans were abusing Cde. Winston Murray saying "no coolie man can run the PNC". When it was overbearing M.P. Mervyn Williams appealed to Corbin for him to put a stop to this nonsense. Corbin looked away and then walked into the building without saying anything.
That is the same gentleman when, after the election for Leadership of the PNCR, reporters published an article about the rigged election emanating from the Sophia headquarters, he remained silent.
If readers could recall, reporters mentioned in one of their articles that they cornered a guy and asked him who he was supporting and he said Corbin.
Lo and behold when they looked at his delegate card and asked him his name, it was different from what the card had. After the election there were lots of accusations of rigging, and those who were fingered in the rigmarole business were always writing letters asking for proof.
Now is the time for the likes of Dr.Richard Van West-Charles, Mervyn Williams, Aubrey Norton, and Vincent Alexander to come out publicly and call names. Because of their silence the party in which they grew and which they nurtured is being raped and killed slowly.
Finally, I would be grateful if the writer could say why the gentleman who Paul Seecharran is trying to praise, did not step down from the leadership of the party that he is hell bent on destroying.
Mr. Corbin never accomplished anything that his supporters could boast about. The writer must have really come from next world to praise a hypocrite and not finding time to praise someone who is worthy of it.
Cde. Winston Murray is gone but people will always talk of his achievements and until I die, I am quiet sure I will not see another like him.
Allisa Bacchus
Jun 27, 2016Beats Raynauth Jeffrey in sprint home By Franklin Wilson Professional cyclist, Foundation and Continental Cycle Club's Geron Williams defied the odds and his rivals from Team Coco's and Team...
Jun 27, 2016
Jun 27, 2016
Jun 27, 2016
Jun 27, 2016
Jun 27, 2016
Jun 27, 2016
If there is any citizen living in the land today who is over-zealous over the current elephant dung that sits on top... more
There has been little attempt by the City Council to explain why in the parking meter deal it settled for a mere 20%... more
By Sir Ronald Sanders The 12 English-speaking independent countries of the Caribbean Community (CARICOM) have at the... more
I live in a country where people do two things very well; they complain a lot and they point fingers at everyone who... more


text:
Ralph's machine-demolishing photo of Ichabod Crane's Motor Toon Skid
The Ichabod Crane's Motor Toon Skid queue is like an adventure inside Toontown. The attraction motors are formed like Bertie the Cab, and when you drive you can skid them by spinning the headlights.
You shouldn't try Pirate Mode
© copyright 2011–2016
A Pants Aflame production


text:
Facing eviction, embattled dairy farmer Allan Crafar, has lodged an application at the High Court in Rotorua to force the receivers of his farms to hand over a number of documents.
Mr Crafar told the National Business Review this afternoon the application for discovery of documents was lodged in an effort to obtain information about how the receivership of his family's 16 dairy farms was being handled, including how decisions were being made and who was making them.
Also today, the Crafars were told to hand over a tenancy agreement for their home.
Receiver Michael Stiassny of KordaMentha said the family was saying they have a document that includes an arrangement to stay on the property for life.
Mr Crafar said the tenancy agreement was between the family and one of the family's companies, Plateau Farms, which actually owned the house.
Mr Stiassny said the receivers had not been made aware of the document before last week.
He added that if the Crafars plan to request documentation through the courts, the receivers would clearly comply with any requirements.
Meanwhile, the Crafar interests have until August 6 to prove they have the money to retire more than $200 million of debt that led to receivers KordaMentha being called in last October.
In addition, $50,000 for security of costs needed to be made available if an application for an injunction to stall the sale of the farms was to proceed at the High Court in Auckland.
This article is tagged with the following keywords. Find out more about MyNBR Tags
NZ Market Snapshot
|Gold Index
|Oil Brent
|Oil Nymex
|Silver Index
Most Popular
- MARKET CLOSE: NZ stocks rise along with global markets; Metro Glass, Xero, A2 lead gainers
- Bill English says NZ well placed to deal with Brexit turmoil
- Government ditches private partner to build Christchurch Convention Centre alone
- NZ dollar gains as Brexit-induced volatility eases
- StretchSense attracts investment from Japanese e-commerce giant
Most listened to
- Google tax: Spark boss Simon Moutter says everything's above board with Southern Cross' use of tax-haven Bermuda
- Diversity advocate Adriana Gascoigne says companies with women on their boards are worth more
- The Brexit Special Edition of Foreign Affairs Scope with Nathan Smith
- In his Editor's Insight Nevil Gibson sees the worst Brexit fears realised
- The Australians doing it better? Chapman Tripp partner Roger Wallis explains


text:
22700 embossing folder case
2000 Sets (Min. Order)
eva case for power bank
US $1-3 / Piece
500 Pieces (Min. Order)
cd case
1000 Pieces (Min. Order)
China factory price DJI inspire 1 case professional protective case for dji inspire one
US $30-150 / Piece
1 Piece (Min. Order)
Round retractablee tape measure factory custom steel measuring tape ABS case
US $0.3-1.5 / Piece
6000 Pieces (Min. Order)
360 degree rotating case for Asus TF101 cover for ASUS Eee Pad Transformer TF101 case
US $4-4.5 / Piece
10 Pieces (Min. Order)
Go to Page
Prev 1 Next
Haven't found the right supplier yet ? Let matching verified suppliers find you. Get Quotation NowFREE
Do you want to show 360 degree cases for asus tf101 or other products of your own company? Display your Products FREE now!
Related Category
Product Features
Supplier Features
Supplier Types
Top 3 Markets
Recommendation for you
related suppliers
related Manufactures


text:
David Ortiz not quite sure he will be hitting Monday
|03.16.13 at 3:42 pm ET
PORT CHARLOTTE, Fla. ' Friday, Red Sox manager John Farrell said that if David Ortiz's injured heels continued to progress the designated hitter could resume some baseball activities Monday.
When asked about the timetable Saturday afternoon, Ortiz wouldn't commit one way or another, simply saying, 'Getting there.'
When asked if he felt better than when he initially stopped activities due to inflammation in both heels, Ortiz responded, 'Probably.'
He later added when asked what was planned, 'Once I get better, I'll have an idea. But I'm not there yet.'
Earlier Saturday, Farrell reiterated that the plan heading into the season without Ortiz was to rotate players in the DH position according to matchups.
One possibility, Ryan Lavarnway, was broached by Farrell prior to the Red Sox' game against the Rays.
'For guys that haven't been strictly a DH, that's a challenging routine for them,' the manager said. 'Particularly a catcher who's in the flow of every pitch thrown and then you got nothing, being completely idle and walk up to the plate a little more cold. We haven't settled on any one combination or front-runner for that matter on who would be in David's slot. We know it's going to be a match-up situation.'
Farrell said he hasn't talked to Jarrod Saltalamacchia about the possibility of serving as a part-time designated hitter. The catcher spent 13 games at DH in 2012 after Ortiz was shut down with his Achilles tendon injury.
'We've focused on a number of things behind the plate and have been very clear that he's our lead catcher and don't want to veer off of that,' Farrell explained.
Latest from Bleacher Report
- Updates on Red Sox Outfielder Chris Young Injury's
- Moncada Has a 'Following Everywhere We Go'
- AL ERA Leader Steven Wright Filling Red Sox's Need for Co-Ace
- Rusney Castillo Clears Waivers, Sent to Triple-A: Comments, Reaction
- Ted Williams 'Would Have Loved' Ortiz Hitting HR No. 521
- Prospect Profile for Red Sox's 1st-Round Pick Jason Groome
- Can Red Sox Make Real Run at 1,000 Runs in 2016?
- Cup of Coffee: Ball great again, Benintendi stays hot
- Cup of Coffee: Espinoza, O'Sullivan impress on the mound
- SoxProspects Featured Video: Sam Travis
- Cup of Coffee: GCL opens season, Washington, Moncada mash
- Scouting Scratch: Kevin Steen, Enmanuel De Jesus and more Lowell pitchers
- Cup of Coffee: Beeks, Dubon debut at Double-A, Lowell wins again
- Sources: Mauricio Dubon promoted to Portland
- Scouting Scratch: Michael Kopech
- Cup of Coffee: Moncada stays hot in Portland; Lowell improves to 6-0
- Cup of Coffee: Moncada makes Double-A debut; Owens dominates for PawSox


text:
Los Angeles Register Recorder County Clerk Office
1. Los Angeles County Cemetery
It has the best customer service bar b q man is so respectful,helpful and I cant wait to go back and visit my loved one again and buy a memorial for…
2. WIC State Agency Call Center
Fast service, Nice workers..
3. Los Angeles County
Probation is nothing nice to be on. However, if you or some one you know is on Probation. I suggest you follow all the conditions of your probation …
4. Ariel Adonay Zuniga Amador
EL CONSULADO GENERAL DE HONDURAS EN LOS ANGELES HA CAMBIADO SU TELEFONO Y CORREO ELECTRONICO: TEL: (213) 995-6406 FAX: (213) 995-6407 E-MAIL: c…
5. Internal Revenue Service
Wait times are long but the staff is attentive, polite, friendly and knowledgeable. Also convenient parking close by although make sure to put more …
6. City of Los Angeles Code Enforcement Program
7. Los Angeles County Juvenile Connecticut
The worst system. I got a traffic ticket and paid it on line (and paid the unreasonable $10. extra for the "administrative fees") because …
8. L A County Probation Dept
I phoned this office and explained a situation I was having with someone on probation. the gentleman who answered the initial phone call was rude, w…
9. Los Angeles Tax & Permit Div
201 N Main StLos Angeles, CA 90012
10. Civil Defense Ofc
200 N Spring St Ste 224Los Angeles, CA 90012
11. Planning Commission
200 N Spring St # 550Los Angeles, CA 90012
12. City Town Village Township Government 15th District
200 N Spring St Ste 425Los Angeles, CA 90012
13. Urban Design Studio
200 N Spring St # 705Los Angeles, CA 90012
14. Cultural Heritage Board
200 N Spring StLos Angeles, CA 90012
15. St of Ca Managed Health
107 S BroadwayLos Angeles, CA 90012
16. Los Angeles Field Office
255 E Temple St # 1273Los Angeles, CA 90012
17. Coordinators Office
210 W Temple St # 13-305Los Angeles, CA 90012
18. La Cnty Grand Jury
210 W Temple St Ste 13-303Los Angeles, CA 90012
19. L A County Criminal Appeals
210 W Temple St Ste M3Los Angeles, CA 90012
20. County Of Los Angeles
210 W Temple St # Fl 18Los Angeles, CA 90012
21. Government Offices United States
312 N Spring St Ste 754Los Angeles, CA 90012
22. Read Debbie
312 N Spring StLos Angeles, CA 90012
23. Lisa Gonzales
312 N Spring StLos Angeles, CA 90012
24. Child Custody Evaluations-Los Angeles County Of Superior Court
111 N Hill St Ste 123Los Angeles, CA 90012
25. Los Angeles Cnty Probate Atty
111 N Hill St # 258Los Angeles, CA 90012
26. Board Of Court Appeal
300 S Spring St # 2217Los Angeles, CA 90013
From Business: Established by a constitutional amendment in 1904, the Courts of Appeal are California's intermediate courts of review. It reviews final judgments of Superior Court…
27. La County Chief Executive Ofc
222 S Hill St # 5Los Angeles, CA 90012
28. County of Los Angeles
222 S Hill St # 5Los Angeles, CA 90012
29. Public Integrity Division
320 W Temple St # 766Los Angeles, CA 90012
30. Los Angeles County Planning
320 W Temple St Ste B35Los Angeles, CA 90012


text:
It's a tee. It's a dress. It's the new Graphic T-shirt Dress.
305 case farm ih rural sunset tractor
Wow! What a beautiful place! Gorgeous job! =) Wishing you many holiday sales!Sisaro
10% off
for joining the Redbubble mailing list
Receive exclusive deals and awesome artist news and content right to your inbox.
Free for your convenience.


text:
Sorry! The position you're looking for is no longer available. Check out other Public Relations internships in New York, NY below.
Results 71 — 80
Sort By:
Public Relations/Social Media Internship
Khirma Eliazov — New York, NY
Luxury handbag company, Khirma Eliazov, is interested in finding interns who have an interest in accessories design and finding out how a small company works. The focus of this internship would be des…
Application Deadline: Available Year-round
Public Relations Intern
Alpaytac — New York, NY
Alpaytac Marketing Communications/Public Relations is a full service public relations firm headquartered in Chicago. We have 18 full-time employees on staff and have offices in Los Angeles, Washington…
Application Deadline: Available Year-round
Manhattan PR agency seeks part time / full time interns (Flatiron)
double E communications — New York, NY
Manhattan PR agency seeks part time / full time interns (Summer & Fall 2016) Description and Requirements: Candidates: - Available Monday-Friday, 9a-5p (can be flexible to work with class schedule); m…
Application Deadline: Available Year-round
Public Relations Intern (unpaid)
Royal Jets INC — New York, NY
Public Relations internship / Royal Jets INC Description Royal Jets INC has an immediate need for a Public Relations intern to join our growing team. This position offers the undergraduate college stu…
Application Deadline: Available Year-round
Authentic Brands Group — New York, NY
Authentic Brands Group is a brand development company that manages a portfolio of iconic consumer brands in the fashion, sports, celebrity and entertainment. For more information visit www.abg-nyc.com…
Application Deadline: Available Year-round
PR Intern
YaYa Publicity — New York, NY
Hip, cool, midsize Public Relations Firm specializing in entertainment, fine jewelry, swim, and fashion is looking for outgoing, proactive, personable, organized, bright, and energetic people to join …
Application Deadline: Available Year-round
Public Relations Intern
Spread The Purple — East Orange, NJ
Spread The Purple, a small nonprofit organization handling busy clients, is looking for an intern interested in learning all aspects of the public relations field. It's essential that applicants to th…
Application Deadline: Available Year-round
PR / Editorial Intern
iQ License — New York, NY
We are looking for fall college interns to help us with public relations and editorial activities, including managing and drafting press releases, preparing feature pieces for publication, and corresp…
Application Deadline: Available Year-round
Marketing / Public Relations Internship
The Clique Travel and Social Alliance — Virtual
The Clique Travel and Social Alliance is an Exclusive Travel Network for Women who enjoy Traveling, but don't always want to go 'SOLO'! Our extensive network of premier suppliers across the globe ensu…
Application Deadline: Available Year-round
Public Relations & Media Internship
LaBelleLaDiva Enterprises LLC. — New York, NY
LaBelleLaDiva Enterprises LLC. is looking for Media Interns to join our prestigious team! Looking for an internship to gain experience in media for Sports, Fashion or Music? This may be the opportunit…
Application Deadline: Available Year-round


text:
Congresswoman Marcy Kaptur, Representative of Northern Ohio's Ninth Congressional District, was the Sponsor and Principal Speaker for the naming ceremony for the tugboat HANDY-THREE at Great Lakes Shipyard.
"She is here today because of her sponsorship and commitment to small shipyards," remarked Ron Rasmus, President, The Great Lakes Group. Kaptur has championed the passage and funding of the Assistance to Small Shipyards Grant Program which makes grants for capital and related improvements for qualified small shipyard facilities to foster efficiency, competitive operations, and quality ship construction and repair. Great Lakes Shipyard has been the recipient of such grants.
The HANDY-THREE is the third of a class of Handy Size tugboats designed by Jensen Maritime Consultants, Seattle, Washington and built at Great Lakes Shipyard. Specifically for harbor and coastal towing, the "HANDYSIZE" Class fills the market niche in the 2,000 - 4,000 horse power tug market for harbor work, fireboats, and construction operations as well as for coastal barge towing. All three boats have been sold to Caribbean and Central American buyers in Great Lakes Shipyard's competition with other international shipyards.
Maritime Today
The Maritime Industry's original and most viewed E-News Service
Last December, MAN Engines introduced its latest generation in-line six-cylinder engines for workboats. Now the first MAN D2676 LE443 engine off the production
Two Damen ASD 3212 tugs for towage and salvage specialist Multraship have been named on June 24 at Terneuzen, the Netherlands.
The Multratug 29 and Multratug 30,
Silver Ships, Inc. has recently delivered a 34-foot custom designed and purpose-built all-welded aluminum fire/rescue boat to the Islip Fire District on Long Island, New York.
The Red River was closed Sunday from mile marker 40 to mile marker 42, after a barge reportedly ran aground and was protruding into the channel near mile marker 41, according to the U.
Intermarine, LLC, transporter of project, breakbulk and heavylift cargo, said it has transported four coke drums on the MV Ocean Freedom from Gijon, Spain, to New Orleans, La.
Cathelco have supplied a ballast water treatment (BWT) system for the C.C. Pacifique, a steel barge which is being converted into a cable laying vessel for Coastal Carriers BV of the Netherlands.
The U.S. Coast Guard is responding to a report of a sheen in Lake Ontario near the Fitzpatrick Nuclear Power Plant in Scriba, New York, approximately 10 miles northeast of Oswego, New York, Sunday.
The Interlake Steamship Company's M/V Lee A. Tregurtha sailed today from Fincantieri Bay Shipbuilding Company in Sturgeon Bay, Wisc., becoming the shipper's third
U.S.-flag Great Lakes freighters (lakers) moved 9.5 million tons of cargo in May, a decrease of 12.5 percent compared to a year ago, the Lake Carriers' Association (LCA) reported.


text:
Echo Park Reviews
Dennis Schwartz
Ozus' World Movie Reviews
Ozus' World Movie Reviews
January 21, 2004
Offers sympathy for the outcasts and freaks living on the fringe of American society.
Full Review | Original Score: C+
Philip Martin
Arkansas Democrat-Gazette
Arkansas Democrat-Gazette
November 1, 2002
| Original Score: 3/5
Top Critic
Roger Ebert
Chicago Sun-Times
Chicago Sun-Times
January 1, 2000
Full Review | Original Score: 3/4
Scott Weinberg
Apollo Guide
Apollo Guide
February 18, 2002
There's a lot of good humour here and you're left feeling as if you've been eavesdropping on some particularly fascinating (and very pleasant) next-door neighbours.
Full Review | Original Score: 75/100
Frederic and Mary Ann Brussat
Spirituality and Practice
Spirituality and Practice
August 26, 2004
Presents vignettes of yearning individuals in Hollywood who dream of making it in show biz.
Brandon Judell
March 3, 2003
| Original Score: 4/5
Emanuel Levy
July 14, 2005
| Original Score: 3/5
Top Critic
Dave Kehr
Chicago Reader
Chicago Reader
January 1, 2000
Top Critic
Vincent Canby
New York Times
New York Times
May 20, 2003
Top Critic
AV Club
August 16, 2001
May 24, 2003
Top Critic
Time Out
January 26, 2006


text:
Never miss a job. Get new jobs emailed to you daily.
We have removed 3 job postings very similar to those already shown. To see these additional results, you may repeat your search with the omitted job postings included.
$102,829 per year
$8,569 per month$2,057 per week$411 per day$51.41 per hour
1.4% higher than national average
Estimated salary from 132 jobs


text:
This member hasn't filled in their description.
kkerner's FML badges
You are connected to FML via the mobile site or an app. How modern.
It's in the can
Hey, you uploaded your photo, and you're cute as a kitten!
kkerner's favorite FMLs
Today, feeling lonely after my recent breakup, I put on my nicest clothes and went out clubbing with a few friends. I brought a guy back to my place, and we got intimate. It was going well, until he took off my push-up bra, then panicked and drunkenly asked, "Where'd they go?!" FML
by chase / 01/24/2013 at 7:54pm / New Zealand (Auckland) / Intimacy
Today, my boyfriend of three days met up with me at the movie theater, sporting a crude tattoo of my face on his cheek, along with a love heart and the word "forever." Looks like I'm single again. FML
by maybe dead in a day / 01/20/2013 at 2:28pm / United States (Minnesota) / Love
Today, at a restaurant, I happily watched as my boyfriend of three years got down on his knees and proposed to me. Before I could say yes and hug him, a girl flung herself at him, kissed him and shouted, "Yes!" With us still highly confused, she then ran away. FML
by Anonymous / 12/30/2012 at 1:29am / Canada (Alberta) / Love
Today, my fiancé and I were fooling around when his foreskin ripped and bled all over the place. After a trip to the emergency room, the doctor told us no sex for 6 weeks to let it heal. We're getting married and going on our honeymoon in 2 weeks. FML
by bleh / 11/23/2009 at 5:29pm / United States (Massachusetts) / Intimacy
- 1
- « Previous page
- Next page »
- Today, I found out at the ripe age of 24, I may never have children due to what my doctor said were… Today, while changing my 10 week old baby's nappy and everything was going fine as usual. Just as I… Today, after getting out a low security psychiatric unit two weeks ago and returning to work after…


text:
Latest: Microscope Stage for Precision Mapping of Large Samples
Latest: Modern Sample Preparation Solutions Bring Mass Spec to the Clinical Research Lab
Latest: Merck Expands Industry-Leading Emprove ® Program to Include Filtration and Single-Use Pr...
Latest: New Software and Cloud Solutions Provide Users with Advanced Customization
Latest: Learn How the Thermo Scientific™ Virtuoso™ Vial Identification System Can Improve Laborat...
Latest: Flexible Format Radiation Resistant Lens
Latest: Hydraulic Mini-Pellet Press for 7mm Pellets
Modern Sample Preparation Solutions Bring Mass Spec to the Clinical Research Lab
New Centrifuge Technology Streamlines In Vitro Studies at University of Florida College of Veterina...
Microscope Stage for Precision Mapping of Large Samples
New Cell Culture Plate Eliminates Edge Effect During Extended Incubation
Latest Clinical Downloads Available from the SelectScience® Library
Exclusive Videos from analytica 2016
Protein Fibrillation Studied with QCM-D using Q-Sense Instruments
Analysis of Nanotoxicity: Particle Characterization & Centrifugation
Automated Target Enrichment using SeqCapEZ DNA Kits on ACSIA NGS Capture Edition
Detection of Dioxins in |Food and Environmental Samples using APGC-MS/MS
Sulfite Content in Shrimp
Active Pharmaceutical Ingredient Analysis of Ibuprofen According to USP 38
Clinical Chemistry Analyzers
Next Generation Sequencing
Microplate Readers
DNA and RNA Purification
Clinical Laboratory Automation
Immunoassay Analyzers
AACC (American Association fo...
31 Jul 2016-04 Aug 2016
18 Oct 2016-22 Oct 2016
PEGS Europe
31 Oct 2016-04 Nov 2016
Neuroscience 2016
12 Nov 2016-18 Nov 2016
Oxidized oils cause foul odor and rancidity in food products. Phenolic antioxidants and ascorbyl palmitate are commonly used in food to prevent the oxidation of oils. This application note from PerkinElmer presents a UHPLC method for the analysis of edible oils to determine the type and amount of ten different antioxidants. The Flexar FX-15 UHPLC and Restek Ultra II C18, 1.9 µm 50 x 2.1 mm column resolved all antioxidants in about three minutes.
Download this Article
Flexar FX-20 UHPLC


text: We're sorry, Personalised Tweet Me Mug is no longer available Why not try one of these instead? Ceramic Letter Dish by Letteroom £12.95$26.30€17.23$19.08 Personalised Map Mug With Choice Of Styles by British and Bespoke £14.95$30.36€19.89$22.03 Spotty China Letter Mug by Letteroom £13.95$28.33€18.56$20.56 Family Cereal Bowls by Letteroom £9.95$20.21€13.24$14.66 Personalised Tweet Me Mug by When I Was a Kid £12.50$25.39€16.63$18.88 This item is no longer available This item is not available for delivery to United States. twitter name? @________ (required) we're sorry we're sorry, This item is no longer available buy a gift voucher related categories home > kitchen > tableware > crockery & chinaware by When I Was a Kid Location: Northants, GB read seller's profile see seller's complete range more items from this seller Personalised Wooden Name Train by When I Was a Kid £5$10.15€6.65$7.37 Canvas Tipi Play Tent by When I Was a Kid £69$140.15€91.82$101.71 Personalised My First Money Box by When I Was a Kid £18$36.56€23.95$26.53 Personalised Wooden Train Track by When I Was a Kid £39.50$80.23€52.56$58.22 Traditional Rag Doll Poppy by When I Was a Kid £14$28.43€18.63$20.63 Canvas Tipi Play Tent Extra Parts Only by When I Was a Kid £6$12.18€7.98$8.84 view all you may also like PRODUCT DETAILS CUSTOMER REVIEWS DELIVERY RETURNS Made in Britain can be personalised ask seller a question save for later Do you know someone that loves to tweet? These fantastic personalised mugs are sure to bring a smile to any tea break. Hand finished at our studio in Northanys, this mug features TWEET ME in scrabble letters but the twist comes from having 'YOUR' twiiter name on the mug as the feature 8cm diameter, 9cm tall and 325ml capacity the mugs are dishwasher safe product code: 69101 1 customer review read about our product rating system ratings guide trusted independent customer feedbackforum excellent bad good poor This product is dispatched to you by When I Was a Kid. These mugs are made and hand finished to order so please take care to get the spelling of the name correct. MAINLAND UK order now and receive this item by price Standard Delivery Monday 4 July £4.75$9.64€6.32$7.17 NON-MAINLAND UK estimated delivery price Northern Ireland 2-5 working daysfrom dispatch £4.75$9.64€6.32$7.17 Other UK Islands 2-5 working daysfrom dispatch £4.75$9.64€6.32$7.17 INTERNATIONAL DELIVERY estimated delivery price Not currently available for this region You have 28 days, from receipt of cancellable goods, to notify the seller if you wish to cancel your order or exchange an item. Please note: goods that are personalised, bespoke or made-to-order to your specific requirements, perishable products and personal items sold with a hygiene seal (cosmetics, underwear) in instances where the seal is broken are non-refundable, unless faulty. Read more about our returns policy.Should you choose to return or exchange your order you will need to deliver the item(s) to the UK, where this seller is based. When I Was a Kid's return policy Please see notonthehighstreet.com's returns policy.


text:
Combo Discount:
Savings of: $3.77 (36% off)
Sorry, this product is no longer in stock.
Posted by Reviewer2515622 on Mar 22, 2008
i dont kno its for what reason..but i had been taking the fenugreek supplement for a week and then i started taking blessed thistle along with it...soon after 4-5 days i had red rashes on some parts of my body...i m not sure of the reason...but as i m breastfeeding my baby did not wanted to take any chances,so i stopped both for somedays.....although my milk suppy has increased considerably,i think i will again start up with both the supplements..and give another review and also rate the herb accordingly.
Was this review helpful to you?


text:
Time Person of the Year: Barack Obama
December 19, 2012
Time had dozens of contenders for Person of the Year, as it does every December.
Apple CEO Tim Cook, who succeeded the late Steve Jobs and kept the company on track following the late founder's vision.
Malala Yousafzai, the schoolgirl shot in the head by the Taliban because she dared to want to learn.
The discoverers of the "God Particle," a concept so complex that most of us don't really understand it.
But in the end Time picked the most obvious choice: Barack Obama, who was elected to a second term as president despite the snail-like pace of the economic recovery.
Time editors announced the choice this morning on NBC's "Today."
In explaining the choice, Time wrote in its introduction: "There has been much talk of the coalition of the ascendant — young people, minorities, Hispanics, college-educated women — and in winning re-election, Obama showed that these fast-growing groups are not only the future but also the present."
Tags: America, barack obama, economic recovery, following, God Particle, magazines, Malala Yousafzai, person of the year, steve jobs, the choice, time, time inc, time person of the year
Related News
Salt Lake City: Revving up for the Olympics
Weekend TV: Oodles of Olympic qualifying
Yes, hate watching is a thing and it's important
BET Awards take a tumble on Twitter
Hispanic TV: 'El Señor de los Cielos' flies high
NBC wins Tuesday with 'Talent' and trials
This week's broadcast ratings
Programming blog: What's canceled and renewed
This week's cable ratings
Univision and Copa America Centenario: Olé
Cable overnights: Shark Week bites off big numbers
'Game of Thrones': The bigger ratings story
What you need to know about black radio
- Karen Mawhinney, Kat Shafer and Jeff Hoffman rise at Erwin Penland
- Michael Angelovich and Danika Kirvin rise at Zimmerman Advertising
- Chris Watling becomes head of production at Droga5 London
- Nina L. Diaz rises to head of reality programming at VH1
- Paul Ricci becomes head of alternative at VH1
- Rory Levine and Tessa Jordan join CMT
- Mark Cuban hosting AXS TV summer concert series
- Mario Lopez launching Awestruck channel on YouTube
- 'The Daily Show' correspondent Jessica Williams exiting
This week's broadcast ratings
This week's cable ratings
This week's top-rated movies, songs and books
This week's daypart ratings
This month's digital traffic data: May 2016
This month's new media traffic data
Associate media director position in LA
Media assistant opening in Northern Virginia
Freelance broadcast planner/buyer available
Assistant media buyer job in Fort Worth
Needed in Louisville: In-house media buyer


text:
World Bulletin / News Desk
The commander of US special forces in Afghanistan has suspended training for all new Afghan recruits until Afghan soldiers are re-investigated for ties to insurgents, The Washington Post reported late on Saturday.
The suspension comes in response to the killing of at least 45 foreign troops this year by their Afghan colleagues.
The newspaper said the re-vetting process will affect more than 27,000 Afghan troops.
"We have a very good vetting process," the paper quotes an unnamed senior special operations official as saying. "What we learned is that you just can't take it for granted. We probably should have had a mechanism to follow up with recruits from the beginning."
NATO has some 130,000 troops occupying Afghanistan.
Most of the NATO troops are set to withdraw by the end of 2014 in a US-designed transition process that will put Afghan security forces in charge of security.
Police say insurgents suspected in latest fatal attack to hit troubled region
UN says progress slow on Sri Lanka's post-war reconciliation
Over 3,800 turn themselves in to police in southern Mindanao alone ahead of President-elect Rodrigo Duterte's inauguration
North Korea responds aggressively to 'military provocation,' as major parliamentary gathering gets underway in Pyongyang
55 Cambodia National Rescue Party lawmakers boycotting assembly since police failed attempt to arrest their deputy leader
7 Indonesians among 13 crew travelling on tugboat when attacked by Filipino gunmen last week
PM Abe's gov't discusses how to lessen UK referendum's fallout on Japanese economy as Tokyo prepares for July 10 polls
Junta leader insists won't follow example of UK's Cameron by resigning if Thais reject draft charter in Aug. 7 referendum
- Police believe suspects behind Sunday's car bombing in Muslim south were involved in blast at mall car park last year
Second finance minister resigns for 'personal reasons' as PM announces 3rd cabinet reshuffle since 2013 election
Activist calls for transparency over why officials were transferred in province notorious for refugee rights abuses, graft
SKorean leader addresses concerns on internal division as government highlights importance of firm handling with North Korea
The southern area of Thailand is plagued by insurgency, although the shooting not thought to be related to the insurgency
A Singapore Airlines plane en route to MIlan turned back to Singapore two hours into journey after reported engine problem
It has been one year since the financial scandal has rocked Malaysia with Prime Minister Najib Razak standing taller than despite an an avalanche of investigations, outmanoeuvering opponents, and boosting his control with a pair of recent election wins.
Officials said the strikes began in the last week and were against extremist targets in the southern part of the country.


text:
Archive of: Blog
State Senate committee meets at Port of Tacoma
Yesterday, the Washington State Senate Government Operations and Security Committee met at the Port of Tacoma to consider in depth the challenges facing the state's ports, as well as how those challenges are affecting the job picture.
|June 2016
New CFO at Saint Martin's University
Edward J. Barton has been named the new chief financial officer at Saint Martin's University in Lacey.
|June 2016
Back to court for State Auditor
A new trial awaits State Auditor Troy Kelley, who escaped a six-week trial last month with a hung jury.
|May 2016
42-year-old Tacoma Towing adding U-Haul service
Tacoma Towing, which has been operating out of Lakewood since 1974, will be adding a new service: U-Haul.
|May 2016
Olympic Panel Products closing soon with 217 layoffs
The long awaited news that Olympic Panel Products in Shelton would be closing down has finally come.
|May 2016
Good Sam Hospital adding 66 beds
According to a Certificate of Need Application with the State of Washington Department of Health, the hospital is looking to add 66 acute care beds to the hospital.
|May 2016
Olympia state park could see private development
In the near future, Millersylvania State Park in Olympia, could see private development.
|May 2016
Proposal would accelerate light rail to South Sound
After receiving almost 35,000 survey responses and 2,300 written comments, Sound Transit rolled out proposed changes that would dramatically speed expansion of light rail.
|May 2016
State Farm commits another $20K to Summer Jobs 253
State Farm has awarded a $20,000 grant to the City of Tacoma for the Summer Jobs 253 program for the second consecutive year.
|May 2016
McMenamins almost ready to transform old Elks Lodge
Soon, the McMenamins brothers will begin transforming the 101-year-old building into their vision: a 46-room hotel.
|May 2016


text:
1135 Terminal Way # 509
Reno , Nevada
United States , 89502-2121
Be sure to check out the pet businesses near you!
Be sure to check out the dog parks near you!
These beautiful pewter leash hooks are perfectly designed for the hallway of a pet lover. You will never misplace your keys or leash again.
Pet podcast #15 is a short podcast that offers 2 easy photo tips to improve your pet photography regardless of your photography experience or the camera you have.
You can download this pet podcast directly by clicking the preceding link …
Copyright © 2000-2016 Pets.ca. All Rights Reserved. | Copyright & Legal Statement | Privacy Policy & Security


text:
Lives in Detroit / / midwest / USA, MI, United States
Works as a Web Design / Photography
Has a website at http://www.ScapeFilms.com
Joined on Aug 18, 2002
About me:
Hey everyone I've been shooting since 1995 and love it. I now do it semiprofessionally and that's great fun. Check out my galleries for some of my work.
roxics has not submitted any challenge entries yet.


text:
List all words ending with gillies
Only the word gillies was found, nothing longer.
Some random words: graal feal jiao eel rape kirigami wo
This is not a dictionary, it's a word game wordfinder.
- Help and FAQ - Examples - Home
Privacy and Cookies Policy - Share - © Copyright 2004-2016 Morewords.com - 83.806mS


text:
Proteomic assessment of a cell model of spinal muscular atrophy
Deletion or mutation(s) of the survival motor neuron 1
When ES cells were primed with Noggin/fibroblast growth factors (bFGF and FGF-8) in a more robust neural differentiation medium for 2 days before differentiation induction, the efficiency of
SMN-deficient ES cells provide a cell-culture model for SMA. SMN deficiency activates cellular stress pathways, causing a dysregulation of energy metabolism, protein degradation, and cytoskeleton stability.
Spinal muscular atrophy (SMA) is an autosomal recessive disorder with a prevalence of 1 in 6000 live births and a carrier incidence of 1 in 40-50
Genetic analyses of familial SMA indicate that the vast majority of SMA is caused by deletion or mutation(s) of the telomeric copy of the survival motor neuron 1 (
Reduced levels of SMN in SMA patients and animal models result in selective death of motor neurons, indicating that SMN plays a more critical role in motor neurons. Consistent with this indication, SMN has been shown to localize to granules that are actively transported into neurites and growth cones
Several SMA mouse models have been developed in the past decade
Embryonic stem (ES) cells are unique cells derived from the inner cell mass of the mammalian blastocyst. These cells are pluripotent, immortal, and can be differentiated into mature cell types in response to specific cues
In this study, we report on the effects of the differentiation of ES cells derived from the severe SMA mouse model into motor neurons. We developed a new protocol to improve differentiation efficiency from ~25% to ~50%. The differentiated motor neurons were characterized by immunofluorescence and western blotting of neuronal and motor neuron markers. Using proteomic techniques, we determined that multiple signaling pathways are dysregulated in SMA cells.
To establish a cell-culture model of SMA, we initially used the control ES line (HBG3; HB9::eGFP) to optimize
Figure 1
Differentiation of murine ES cells into motor neurons
Differentiation of murine ES cells into motor neurons. The scheme outlines the process of motor neuron differentiation from ES cells (A). Murine ES cells (HBG3) were expanded on a layer of primary mouse embryonic fibroblasts. Undifferentiated stem cells form round clusters (arrows) as they proliferate on top of a feeding fibroblast layer, and they have weak GFP expression (B-B'). Stem cells were separated from the fibroblast layer and plated in low attachment dishes to allow for differentiation. Two-day-old stem cell aggregates (called embryoid bodies, EB, arrows) were induced to differentiate with 1 μM retinoic acid and 1 μM sonic hedgehog agonist SAG for 5 days. Differentiated ES cells have strong GFP expression (C-C'). When plated on poly-DL-ornithine/laminin/matrigel coated plates, the differentiated ES cells extend long neurites after 2 days in culture (D-D'). Representative phase contrast images for 1B-1D are shown on the top and corresponding fluorescent images are on the bottom. Scale bar = 200 μm except as indicated. MN = motor neuron.
The differentiated cells were characterized by the expression of neuronal and motor neuron marker proteins. Western blotting shows that a pan-neuronal marker (neurofilament) and two motor neuron specific markers (Hb9 and ChAT) are expressed in differentiated ES cells but not in the undifferentiated ES cells (Figure
Figure 2
Characterization of motor neuron differentiation
Characterization of motor neuron differentiation. HBG3 cells were induced to differentiate following the procedure as in 1A. Lysates from undifferentiated and differentiated cells were resolved on an SDS-PAGE and transferred to a membrane. The blot was probed with antibodies against pan-neuronal (neurofilament, NF) and motor neuron markers (Hb9 and ChAT). The same blot was stripped and re-probed with anti-ERK antibodies for protein loading control (A). Differentiated ES cells were dissociated and processed for indirect immunofluorescence staining for neurofilament (NF, in red), ChAT (in red), Islet-1 (in red), and DAPI (for nuclei in blue). Scale bar = 50 μm. U = undifferentiated cells and D = differentiated cells.
Proteomic analyses reveal altered protein expression in SMN-deficient ES cells
The SMA ES cells were derived from a severe SMA transgenic mouse that lacks the murine
Figure 3
Reduced expressions of SMN and Gemin2 in SMN-deficient ES cells
Reduced expressions of SMN and Gemin2 in SMN-deficient ES cells. Control (C) and SMA (S) ES cells were separated from feeding fibroblasts and allowed to differentiate
Figure 4
Characterization of differentiation of control and SMN-deficient ES cells
Characterization of differentiation of control and SMN-deficient ES cells. Control (C) and SMA (S) ES cells as described in 3A were processed for western blotting using antibodies against neurofilament (NF), Hb9, and ChAT respectively. The same blot was stripped and re-probed with anti-ERK antibody for protein loading control (A). Signals of three independent experiments were quantified, normalized to ERK, and plotted as for 3B. Differentiated control and SMA ES cells show similar levels of expression for neuronal and motor neuron marker proteins (B). Undiff. = undifferentiated cells and Diff. = differentiated cells.
To identify proteins that are up- or down-regulated in SMN-deficient ES cells, comparative two-dimensional SDS-polyacrylamide gel electrophoresis (SDS-PAGE) analyses of control and SMA ES cells were performed. The differences between control and SMN-depleted protein pools were analyzed using four separate control and SMA ES cell extracts and quantified using DeCyder software (Figure
Table 1
Proteomic identification of dysregulated proteins in SMN-deficient ES cells
Protein Name
Gene Symbol
Fold Change
Chaperonin 6a
Protein folding
FK506 binding protein 4
Protein folding and trafficking
Peroxiredoxin 6
Redox regulation
Stress-induced-phosphoprotein 1
Adaptor protein for Hsp70 and Hsp90
Lactate dehydrogenase B
Brain creatine kinase
Cellular energy homeostasis
Glyoxalase 1
Detoxification of glucose degradation products
Tropomyosin 3
Stabilization of actin filaments
Annexin A5
Cell signaling, Inflammation; Growth/differentiation
Ubiquitin carboxy-terminal hydrolase L1
De-ubiquitination; Ubiquitin ligation; Mono-ubiquitin stabilizer
Cytoskeletal protein
Aldehyde dehydrogenase
Aldehyde oxidation
14-3-3 gamma
Cell signaling; Check point control; Apoptosis
Heat shock protein 90B
Chaperone protein
Heat shock protein 70K
Chaperone protein; Cell proliferation
Figure 5
Comparative 2D DIGE analysis of protein expression in control and SMA ES cells
Comparative 2D DIGE analysis of protein expression in control and SMA ES cells. Control and SMA ES cells were harvested and lysed. Equal amounts of total proteins from wild-type or SMA ES cells were trace labeled with either Cy3 or Cy5. The dyed proteins indicated by the labeled colors were mixed and separated by isoelectric focusing in 7 cm pH 3-10 linear gradient IPG strips, and further separated in a second dimension of 10% SDS-PAGE. Fluorescently labeled proteins were visualized by Typhoon Trio scanner and difference between samples analyzed by the accompanied software. Samples shown in the left and right panels were from different preparations of ES cells and labeled with opposite dyes to demonstrate the reproducibility of the assay. A few abnormally expressed corresponding proteins were marked by arrows. MW = molecular weight.
Figure 6
Abnormal expression of peroxiredoxin 6, tropomyosin, and lactate dehydrogenase in SMN-deficient ES cells
Abnormal expression of peroxiredoxin 6, tropomyosin, and lactate dehydrogenase in SMN-deficient ES cells. Control (C) and SMA (S) ES cells as described in 3A were processed for western blotting using antibodies against peroxiredoxin 6 (PRDX6), tropomyosin (TPM1/3), and lactate dehydrogenase (LDH), respectively. The same blot was stripped and re-probed with anti-ERK antibody for protein loading control (A). Signals of three independent experiments were quantified, normalized to ERK, and plotted as for 3B. Western blotting confirms that SMA ES cells show significantly higher levels of peroxiredoxin 6, tropomyosin, and lactate dehydrogenase expression (**
Figure 7
Western blotting profile of α-tubulin and chaperone-related proteins
Western blotting profile of α-tubulin and chaperone-related proteins. Control (C) and SMA (S) ES cells as described in 3A were processed for western blot analyses using antibodies against α-tubulin, chaperone-related proteins as indicated, and protein loading control ERK. Undiff. = undifferentiated cells and Diff. = differentiated cells.
Western blot analysis reveals specific cell stress-response pathways activated in SMN-deficient ES cells
Our proteomic study indicates that reduced levels of SMN increase expression of stress-response proteins (Table
Figure 8
Elevated expressions of stress-related proteins in SMN-deficient ES cells
Elevated expressions of stress-related proteins in SMN-deficient ES cells. Control (C) and SMA (S) ES cells as described in 3A were processed for western blotting using antibodies against phosphorylated p38 (pP38), p38, phosphorylated JNK (p-JNK), JNK, and TIA-1. The same membranes were stripped and re-probed with anti-ERK antibody for protein loading control (A). Signals of three independent experiments were quantified, normalized to corresponding ERK signals, and plotted as for 3B. SMA ES cells express significantly higher levels of phosphorylated p38, phosphorylated JNK, and TIA-1 proteins (**
Figure 9
Western blotting profile of cell death-related proteins
Western blotting profile of cell death-related proteins. Control (C) and SMA (S) ES cells as described in 3A were processed for western blot analyses using antibodies against Bax, Bad, Puma, p53, and protein loading control ERK. Undiff. = undifferentiated cells and Diff. = differentiated cells.
Cellular stress-response protein p21 is up-regulated in both SMN-deficient ES cells and in spinal cords of SMA transgenic mice
The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell-cycle progression. It has been shown that p21 is induced in response to a variety of stress signals, including genotoxins, oxidants, and metabolic perturbation
Figure 10
Up-regulation of p21 in SMN-deficient ES cells and spinal cord tissues derived from SMA mice
Up-regulation of p21 in SMN-deficient ES cells and spinal cord tissues derived from SMA mice. Control (C) and SMA (S) ES cells as described in 3A were processed for western blotting using antibodies against p21 and p27. The same membrane was stripped and re-probed with anti-ERK antibody for protein loading control (A). Spinal cord tissues from three control (C) and three SMA (S) mice were collected and lysates were prepared. Equal amount of protein lysates were separated on a SDS-PAGE and transferred to a membrane. The blot was probed with antibodies against p21 or SMN. The same blot was stripped and re-probed with anti-ERK antibody for protein loading control (B). Undiff. = undifferentiated cells and Diff. = differentiated cells.
In this study, we have developed an improved protocol for
Differentiation efficiency of control and SMA ES cells were similar, but our study shows that several pathways involved in cell stress-response, energy metabolism, protein turnover, and cytoskeleton stability are affected by low expression of SMN (Table
Although motor neurons are the primary cells affected in SMA, most studies use alternative tissues or cell cultures because of difficulties in obtaining motor neurons from affected animals. Antibodies detect proteins with higher specificity and sensitivity, but such studies are restricted by availability of suitable antibodies for blotting or antibody arrays. By comparison, comparative two-dimensional SDS-PAGE difference gel electrophoresis and iTRAQ analyses can be used to provide unbiased analysis of protein changes. For iTRAQ expression, differences are determined from alterations in quantities of the tags used to label discrete populations of peptides derived from proteins isolated from control and affected cells or tissues. This requires all peptides to be identified by LC/MS/MS. For gel systems, differences in quantities of dye-tagged proteins are determined, and only differentially expressed proteins need to be processed for identification by MS/MS. We detected 1900 spots using two-dimensional SDS-PAGE compared to an iTRAQ study with SMA fibroblasts that detected 2171 proteins
In our proteomic analyses, we also found up-regulation of tropomyosin in differentiated SMA ES cells (Table
Up-regulation of peroxiredoxin 6 in SMA ES cells (Table
Our analyses show a number of pathways that are dysregulated in SMA ES cells but the most important may be induction of cell stress. This cell stress might explain why we see significantly reduced numbers of SMA ES cells compared to control ES cells 5 days after differentiation (C. Wu, D. Whye, W. Wang, unpublished data) and why motor neurons are particularly sensitive to SMN deficiency in SMA patients. Stress-induced cell death is more pronounced in fibroblasts derived from SMA patients compared to age-matched controls
We have derived SMN-deficient ES cells from a severe transgenic SMA mouse which represent a cell-culture model of SMA. Our work indicates that lower expression of SMN activates cellular stress pathways and causes a dysregulation of energy metabolism, protein degradation, and cytoskeleton stability.
Embryonic stem cell culture
HBG3 ES cells were derived from a HB9::GFP (
Differentiation of ES cells into motor neurons
Embryonic stem cells on fibroblast feeder layers were dissociated with 0.25% trypsin/EDTA and plated onto 0.1% gelatin-coated tissue culture flasks. Fibroblasts were allowed to re-attach to the flask, and then the floating ES cell population was collected. Approximately 2 × 106 cells were plated into 100-mm petri dishes containing 10 ml of neural differentiation priming medium containing DMEM supplemented with 15% FBS, 1% MEM non-essential amino acids, 1% GlutaMax-I supplement, 1% penicillin/streptomycin, 1 mM monothio-glycerol, 50 ng/ml Noggin (Invitrogen, Carlsbad, CA, USA), 20 ng/ml bFGF (Invitrogen), and 20 ng/ml FGF-8 (Invitrogen) for EB formation. Media were replaced daily. After two days, aggregated EBs were re-suspended in motor neuron differentiation medium (NITSf) containing basal medium A supplemented with 10% knockout serum replacement (Invitrogen), 1% N-2 supplement, 1% ITS-B supplement (Stem Cell Technologies), 1% ascorbic acid, 1% penicillin/streptomycin, 0.1 mM β-mercaptoethanol, 0.5% GlutaMax-I supplement, 30% D-glucose, 20 μg/ml heparin (Sigma, St. Louis, MO, USA) and 50 μg/ml fibronectin (Stem Cell Technologies) in the presence of 1 μM RA (Sigma) and 1 μM SAG (Calbiochem, Gibbstown, NJ, USA). Media were replaced daily. After 5 days of induction with RA and SAG, EBs were collected, washed with phosphate-buffered saline (PBS), and dissociated in Accumax (Millipore) following the manufacturer's protocol. Cell aggregates were depleted from dissociated EBs by filtering through a BD cell strainer. Single cells were then suspended in NITSf medium supplemented with 10 ng/ml of each BDNF, GDNF, CNTF, and NT-3 and plated at a concentration of 1 × 105 cells per well of 24 well plates that contained poly-DL-ornithine hydrobromide (Sigma)/laminin (Millipore)/matrigel- (BD Bioscience, San Jose, CA, USA) coated coverslips.
Comparative two-dimensional SDS-polyacrylamide gel electrophoresis and mass spectrometry analysis
Undifferentiated and differentiated control and SMA ES cells were lysed in 30 mM Tris, pH 8.5 containing 7 M urea, 2 M thiourea, and 4% CHAPS. Lysates were cleared by centrifuging at 12,000 × g at 4ºC for 10 min. A portion of each sample supernatant was pooled together to create an internal standard. Individual samples were labeled with CyDye DIGE Fluor minimal labeling dyes (GE Healthcare, Piscataway, NJ, USA) at a ratio of 200 pmol Cydye per 50 μg protein according to manufacturer's protocols. For each experiment, two control and two SMA samples were labeled with Cy3, and another two control and two SMA samples were labeled with Cy5. This dye swap was performed to negate any dye preference artifacts in analysis. The internal standard protein pool was labeled with Cy2. Equal portions of control, SMA, and internal standard were mixed and loaded onto IPG strips (BioRad, Hercules, CA, USA) via rehydration in the presence of the same lysis buffer (see above) containing 0.5% Pharmalyte 3-10 (BioRad). Samples from undifferentiated ES cells were run on pH 3-10 linear IPG strips, while differentiated samples were run on pH 3-10 non-linear IPG strips. After isoelectric focusing, IPG strips were equilibrated for 15 min with 75 mM Tris, pH 8.8, 6 M Urea, 30% Glycerol, 2% SDS, and 65 mM DTT followed by 15 min with 75 mM Tris, pH 8.8, 6 M Urea, 30% Glycerol, 2% SDS, and 135 mM iodoacetamide (Sigma). The IPG strips were loaded onto 10% SDS-PAGE gels, and proteins were separated by electrophoresis and imaged using a Typhoon Trio Scanner (GE Healthcare). Changes in levels of individual protein spots were determined using Decyder 6.5 software (GE Healthcare). Statistical significance of the change was determined using a paired, two-tailed Student's
Peptide identities were determined by mass spectrometry. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI TOF MS) was carried out on a 4800 MALDI TOF/TOF Analyzer (Applied Biosystems, Atlanta, GA, USA). Peptide mass fingerprint (PMF) spectra were obtained in positive ion reflector mode with internal calibration for each sample. Four peaks from each spectrum were selected and subjected to further fragmentation for sequence information via tandem MS (MS/MS) with default calibration. Using the search program Mascot (v2.2, Matrix Sciences), PMF and MS/MS spectra were submitted for database searches with the following parameters: NCBInr database, trypsin enzyme, 1 maximum missed cleavage, oxidation of methionines, carbamidomethylation of cysteines, 75 ppm precursor mass tolerance, and 0.3 Da MS/MS fragment mass tolerance. Only protein matches with a probability score below the significance threshold (
Immunocytochemistry and western blot analysis
Immunocytochemistry and western blot analyses were performed as previously described
For western blotting, spinal cord samples were collected at P2-5 from the same colonies of transgenic mice used to derive control and SMA ES cells (see above). All procedures involving animals for collecting spinal cord tissues were performed in conformity with the guidelines of the National Institutes of Health and were approved by the Nemours/Alfred I. duPont Hospital for Children Institutional Animal Care and Use Committee (approved protocol number: NBR-2008-003). Prior to tissue harvesting, the genotype of SMA pups were determined by standard PCR (Jackson laboratories). Lysates from spinal cord tissues and ES cells were prepared, protein concentrations were measured using the BCA assay, and western blot analyses were performed as previously described
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
CW bred mice, collected and genotyped spinal cord samples, performed immunofluorescence studies of differentiated ES cells, and participated in writing the manuscript. DW developed the protocol for ES cell differentiation and participated in western blot analyses and manuscript writing. LG and RWM carried out proteomic work including 2D SDS-PAGE analyses, spot picking, and trypsin digestion. DK contributed ES cells. LC and KHL conducted mass spectrometry studies. WW participated in western blot analyses, overall design of the study, and the writing of the manuscript. All authors read and approved the final manuscript.
We are indebted to Dr. Jeffery Twiss for critical comments on this work. This work was supported by Nemours, a grant (2 P20 RR016472-10) under the INBRE program of the National Center for Research Resources (NCRR), and a COBRE grant award from the NIH (5 P20 RR020173-04) to support the Center for Pediatric Research at the Alfred I. duPont Hospital for Children, Wilmington, Delaware, USA.
Incidence, prevalence, and gene frequency studies of chronic childhood spinal muscular atrophy
Spinal muscular atrophy
The neurobiology of childhood spinal muscular atrophy
Classification of spinal muscular atrophies
Identification and characterization of a spinal muscular atrophy-determining gene
A single nucleotide in the SMN gene regulates splicing and is responsible for spinal muscular atrophy
A single nucleotide difference that alters splicing patterns distinguishes the SMA gene SMN1 from the copy gene SMN2
The SMN complex, an assemblyosome of ribonucleoproteins
Chaperoning ribonucleoprotein biogenesis in health and disease
Spinal muscular atrophy: why do low levels of survival motor neuron protein make motor neurons sick?
Gemin8 is a novel component of the survival motor neuron complex and functions in small nuclear ribonucleoprotein assembly
Unrip is a component of SMN complexes active in snRNP assembly
Ribonucleoprotein assembly defects correlate with spinal muscular atrophy severity and preferentially affect a subset of spliceosomal snRNPs
SMN deficiency causes tissue-specific perturbations in the repertoire of snRNAs and widespread defects in splicing
Active transport of the survival motor neuron protein and the role of exon-7 in cytoplasmic localization
Smn, the spinal muscular atrophy-determining gene product, modulates axon growth and localization of beta-actin mRNA in growth cones of motoneurons
Knockdown of the survival motor neuron (Smn) protein in zebrafish causes defects in motor axon outgrowth and pathfinding
Reduced U snRNP assembly causes motor axon degeneration in an animal model for spinal muscular atrophy
Neurofilament accumulation at the motor endplate and lack of axonal sprouting in a spinal muscular atrophy mouse model
Selective vulnerability of motor neurons and dissociation of pre- and post-synaptic pathology at the neuromuscular junction in mouse models of spinal muscular atrophy
Reduced SMN protein impairs maturation of the neuromuscular junctions in mouse models of spinal muscular atrophy
Embryonic motor axon development in the severe SMA mouse
Impaired synaptic vesicle release and immaturity of neuromuscular junctions in spinal muscular atrophy mice
SMNDelta7, the major product of the centromeric survival motor neuron (SMN2) gene, extends survival in mice with spinal muscular atrophy and associates with full-length SMN
The human centromeric survival motor neuron gene (SMN2) rescues embryonic lethality in Smn(-/-) mice and results in a mouse with spinal muscular atrophy
A transgene carrying an A2G missense mutation in the SMN gene modulates phenotypic severity in mice with severe (type I) spinal muscular atrophy
Spinal muscular atrophy: new and emerging insights from model mice
Embryonic stem cells and prospects for their use in regenerative medicine approaches to motor neurone disease
Stem cells: an overview of the current status of therapies for central and peripheral nervous system diseases
Axonal growth of embryonic stem cell-derived motoneurons in vitro and in motoneuron-injured adult rats
Directed differentiation of embryonic stem cells into motor neurons
Human neural stem cell grafts ameliorate motor neuron disease in SOD-1 transgenic rats
Human neural stem cell-derived cholinergic neurons innervate muscle in motoneuron deficient adult rats
Recovery from paralysis in adult rats using embryonic stem cells
Purmorphamine activates the Hedgehog pathway by targeting Smoothened
Directed differentiation of ventral spinal progenitors and motor neurons from human embryonic stem cells by small molecules
Fibroblast growth factor is a direct neural inducer, which combined with noggin generates anterior-posterior neural pattern
Early posterior neural tissue is induced by FGF in the chick embryo
A truncated FGF receptor blocks neural induction by endogenous Xenopus inducers
Noggin-mediated antagonism of BMP signaling is required for growth and patterning of the neural tube and somite
Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) regulates the level of SMN expression through ubiquitination in primary spinal muscular atrophy fibroblasts
SMN deficiency disrupts brain development in a mouse model of severe spinal muscular atrophy
From receptors to stress-activated MAP kinases
Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation
Protein expression changes in spinal muscular atrophy revealed with a novel antibody array technology
TIA-1: structural and functional studies on a new class of cytolytic effector molecule
Survival motor neuron protein facilitates assembly of stress granules
Fas-activated serine/threonine kinase (FAST) phosphorylates TIA-1 during Fas-mediated apoptosis
Examination of the expanding pathways for the regulation of p21 expression and activity
Valproate and bone loss: iTRAQ proteomics show that valproate reduces collagens and osteonectin in SMA cells
Stathmin, a microtubule-destabilizing protein, is dysregulated in spinal muscular atrophy
The metabolism of lactate
Different atrophy-hypertrophy transcription pathways in muscles affected by severe and mild spinal muscular atrophy
Human tropomyosin isoforms in the regulation of cytoskeleton functions
A role for polyproline motifs in the spinal muscular atrophy protein SMN. Profilins bind to and colocalize with smn in nuclear gems
A role for complexes of survival of motor neurons (SMN) protein with gemins and profilin in neurite-like cytoplasmic extensions of cultured nerve cells
Smn depletion alters profilin II expression and leads to upregulation of the RhoA/ROCK pathway and defects in neuronal integrity
SMN, profilin IIa and plastin 3: a link between the deregulation of actin dynamics and SMA pathogenesis
Plastin 3 is a protective modifier of autosomal recessive spinal muscular atrophy
1-Cys peroxiredoxin, a bifunctional enzyme with glutathione peroxidase and phospholipase A2 activities
Mitochondrial dysfunction in a neural cell model of spinal muscular atrophy
Dysregulation of stathmin, a microtubule-destabilizing protein, and up-regulation of Hsp25, Hsp27, and the antioxidant peroxiredoxin 6 in a mouse model of familial amyotrophic lateral sclerosis
SMN protects cells against mutant SOD1 toxicity by increasing chaperone activity
The role of p53 in neuronal cell death
Neuronal p38 MAPK signalling: an emerging regulator of cell fate and function in the nervous system
p21(WAF1/Cip1): more than a break to the cell cycle?
Transcriptional regulation of the p21((WAF1/CIP1)) gene
Increased susceptibility of spinal muscular atrophy fibroblasts to camptothecin-induced cell death
Inhibition of apoptosis by Z-VAD-fmk in SMN-depleted S2 cells
Involvement of survival motor neuron (SMN) protein in cell death
Knockdown of SMN by RNA interference induces apoptosis in differentiated P19 neural stem cells
Survival motor neuron protein regulates apoptosis in an in vitro model of spinal muscular atrophy
Abolishing Bax-dependent apoptosis shows beneficial effects on spinal muscular atrophy model mice
Restoring Bcl-x(L) levels benefits a mouse model of spinal muscular atrophy
Increased susceptibility of spinal muscular atrophy fibroblasts to camptothecin is p53-independent


text:
A definitive guide to what's recyclable in Philadelphia, plus how to handle the hazardous stuff.
Got it sorted out? Test your skills against these common recycling blunders.
Get notified about updates to your city's recycling program.
Get notified about updates to Philadelphia's recycling program.
This crazy little world of waste
© 2016 Civil Agents. All rights reserved.


text:
Recommended siteA-Z Lyrics Universe
Lyrics/song texts are property and copyright of their owners and provided for educational purposes.
Trapped Lyrics by Young Jeezy
Trapped by Young Jeezy
They still be running, grinding
It was like, you ain't really give a fuck
I'm right here and I'm saying that it's wack sauce
Everything that you do, tryna be the boss
I mean, honestly pursuing it?
It's a trap!
(That's what it's called by the way)
It's a trap night nigga, here we go again
Chill at the door, wide open so I'm going in
Through her bedroom, I could see my mama's tears
Please mama don't cry. you know I hate crying
Music blog
New soundtracks
Follow us on


text:
Late Late Show host Craig Ferguson is coming to the big apple.
Ferguson will be performing at Radio City Music Hall on October 6th. In an interview with the morning show, Ferguson claimed the show will be a bit more personal as well as raunchier than his late night TV show.
Since writing his own novel, screen play, and creating voice overs for television shows such as "Futurama," "American Dad," and "Hercules," Ferguson's career has skyrocketed. This is not Ferguson's first appearance in NYC. A few years back, Ferguson sold out two shows at Carnegie Hall. However, this is Ferguson's first time bringing his talent to Radio City.
Ferguson admitted to rarely having many New York shows on the schedule, so get your tickets while they last.
Tickets are currently on sale.
More from Dan Taylor in the Morning on WCBSFM.com!
Latest from WCBSFM.com >
- Barry Gibb Peps Solo Album 'In The Now'
- Win Tickets For The Beach Boys Coney Island Amphitheater
- Backstreet's Back! Here are the Boys' New (Country) Collaborators
- Seal Joins Montreal Street Performer for Surprise 'Stand By Me' Duet
- Sting and Peter Gabriel: The 'Tantric Twins' Stretch Out in New York


text:
I'm looking for the fastest way to replace a large number of sub-strings inside a very large string. Here are two examples I've used.
findall() feels simpler and more elegant, but it takes an astounding amount of time.
finditer() blazes through a large file, but I'm not sure this is the right way to do it.
Here's some sample code. Note that the actual text I'm interested in is a single string around 10MB in size, and there's a huge difference in these two methods.
import re
def findall_replace(text, reg, rep):
for match in reg.findall(text):
output = text.replace(match, rep)
return output
def finditer_replace(text, reg, rep):
cursor_pos = 0
output = ''
for match in reg.finditer(text):
output += "".join([text[cursor_pos:match.start(1)], rep])
cursor_pos = match.end(1)
output += "".join([text[cursor_pos:]])
return output
reg = re.compile(r'(dog)')
rep = 'cat'
text = 'dog cat dog cat dog cat'
finditer_replace(text, reg, rep)
findall_replace(text, reg, rep)
UPDATE Added re.sub method to tests:
def sub_replace(reg, rep, text):
output = re.sub(reg, rep, text)
return output
re.sub() - 0:00:00.031000
finditer() - 0:00:00.109000
findall() - 0:01:17.260000


text:
Lyrics Depot is your source of lyrics to Skin And Bone by kinks. Please check back for more kinks lyrics.
Skin And Bone Lyrics
Artist: kinks
Album: Muswell Hillbillies
Fat Flabby Annie was incredibly big
She weighed just about sixteen stone
And then a fake dietician went and put her on a diet
Now she looks like skin and bone.
Do the meditation and yoga
And she's thrown away the good food guide
And she's given up the alcohol and pizzas
And the pies and now she looks as if she's ready to die,
You can't see her walk by.
Don't eat no mashed potatoes,
Don't eat no buttered scones
Stay away from carbohydrates
You're gonna look like skin and bone.
Living on the edge of starvation
And she says she's got no appetite
And her father and her mother
And her sisters and her brothers
Couldn't see her when she walked by
She looked like skin and bone.
Don't eat no mashed potatoes,
Don't eat no buttered scones
Stay away from carbohydrates
You're gonna look like skin and bone.
She used to be so cuddly,
She used to be so fat,
But oh what a sin cos she's oh so thin
That she lost all the friends that she had,
She looks like skin and bone
If you look flabby
And you feel overweight,
And you wanna lose a couple of stone,
Take a crash course diet do your daily exercises
And you'll look like skin and bone.
Come on rattle them bones,
Put your hands up to the ceiling,
Bend your hips and touch your toes,
Do your daily exercises,
You're gonna look like skin and bone,
Don't eat no mashed potatoes, etc.
kinks Lyrics
kinks Muswell Hillbillies Lyrics
More kinks Music Lyrics:
kinks - I'm Not Like Everybody Else Lyrics
kinks - Rosemary Rose Lyrics
kinks - Rosy Won't You Please Come Home Lyrics
kinks - Running Round Town Lyrics
kinks - Sitting By The Riverside Lyrics
kinks - State Of Confusion Lyrics
kinks - Things Are Getting Better Lyrics
kinks - Yes Sir, No Sir Lyrics


text:
The Wauwatosa Public Library program, "Blue Dragon Tales: A Remarkable Conservation Story from the Caribbean," for kids ages 5 to 12 has been postponed until Aug. 2, to accommodate the presenter's trip to Grand Cayman Island to further study the iguanas that are the subject of the story.
Instead, children in grade four and higher can attend "Blue Dragon Tales: Fantasy, Myth and Today" from 6:30 to 7:15 p.m. tomorrow at the library. Wauwatosa resident Martin Tierney, a former co-owner of a fantasy board game company and avid reader of fantasy and science fiction, will present a program about dragons. He will trace the evolution of dragons from mythology to popular literature. Registration is not required, but questions can be directed to the Children's Library at (414) 471-8486.
More from News and Features
- Wauwatosa In Brief: Get set for Dozer Day in the Village, July 9
- Wauwatosa Meetings: June 30
- Wauwatosa Village businesses remain upbeat amid ongoing reconstruction
- East Tosa Gran Prix draws racers from across the continent
- Wauwatosa News and Notes: June 30
- Mystery Photo Contest: June 30
- Wauwatosa Senior Meals: Week of July 4
- Wauwatosa Ask Now: Yale Place was once known as Pickle Alley
- Wauwatosa Public Forum: Commercial vehicle parking ban is obsolete
- Wauwatosa Virtual Academy reports consistent enrollment numbers