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Will & Grace 1998 | TV Show Watchlist
Season 2, Episode 5 Polk Defeats Truman
First Aired: November 16, 1999
Grace teaches Karen about budgeting by taking her outlet shopping, while Will sells himself short to land a fickle, filthy rich client (Gary Grubbs). But when the bottom drops out, a vindicated Grace feels bad about coming out on top. Jack: Sean Hayes. Shopper: Elaine Bromka. Grace: Debra Messing.
Guest Cast
Elaine Bromka Elaine Bromka Shopper
Gary Grubbs Gary Grubbs Harlin Polk
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- Premiered: September 21, 1998
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- Premise: Snappy hit about a straight woman and a gay man who are best friends. She's a decorator with a snippy, self-absorbed assistant; he's a lawyer with a flamboyant buddy who has trouble holding a job. The scripts are laced with pop-culture references and in-jo… (more)
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By Running Press Miniature EditionsBuy New: $5.95
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Orbitz To Rent More Hybrid Vehicles
Orbitz Worldwide Inc. announced an agreement with Fox Rent A Car, a rental company that specializes in airport rentals of hybrid and fuel-efficient vehicles. Orbitz Worldwide will offer Fox rentals across all the company's brands, including Orbitz for Business.
The company says that year-over-year, hybrid rentals through Orbitz.com increased 80 percent in July 2008. In addition, a recent Orbitz study found that 35 percent of those surveyed say they are now renting smaller and more economical vehicles as a result of rising gas prices.
The company says that through its own corporate initiatives and eco-travel microsite, it is aiming to offset more than 12 million pounds of CO2 in partnership with CarbonFund.org in 2008.
Last year, the company launched a corporate citizenship initiative to raise environmental awareness, and an eco-travel microsite with environmentally-focused travel tools and content.
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Tuesday, 28 June 2016
Last updated 4 hours ago
Jan 3 2012 | 4:56am ET
AQR Capital Management is ringing in the New Year with three new hedge funds.
The $41 billion quantitative specialist revealed the planned funds in Securities and Exchange Commission filings on Thursday, the second-to-last business day of 2011. It is unclear when the new vehicles will actually launch.
AQR's new funds include a so-called "black swan" tail-risk fund and a global total return fund. The third fund is named for former British Prime Minister Winston Churchill—AQR founder Clifford Asness' favorite historical figure—although its strategy is unclear.
The minimum investment requirement for the three new funds is $5 million.
Jun 16 2016 | 4:19pm ET
Jun 21 2016 | 9:01pm ET
Contrary to concerns that Wall Street isn't as appealing to new graduates as it...
Jun 17 2016 | 1:05pm ET
Over the past year, a large portion of the financial services industry has awakened...


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Product Information for Nintendo The NINJA GAIDEN 3: Razor's Edge
Highlights for Nintendo The NINJA GAIDEN 3: Razor's Edge
Marketing Information
Ryu Hayabusa + Ayane + Wii U = Ninja Awesomeness
The solitary ninja, Ryu Hayabusa, must find a way to lift the deadly curse on his right arm before it destroys him. NINJA GAIDEN 3: Razor's Edge has been reworked to bring to Wii U the truly intense, high-speed challenge and action NINJA GAIDEN fans demand. And this time, the female ninja, Ayane, will join the battle as a new playable character in her own exclusive chapters within story mode. With more weapons and Ninpo types, a new character progression system, a redesigned battle system, a new character, two-player online co-op game play using Ryu and Ayane, and the return of dismemberment, NINJA GAIDEN 3: Razor's Edge improves upon the original NINJA GAIDEN 3 in every way and offers Wii U exclusive features and functionalities.
General Information
License Information
Physical Characteristics
Product Information
- New Playable Character: Ayane - NINJA GAIDEN 3: Razor's Edge adds a new playable character: the female ninja, Ayane, who will be available in her own exclusive new chapters within the story mode.
- Chapter Challenge Mode - In this separate mode, players can progress through any of the chapters in the story mode using either Ryu Hayabusa or Ayane.
- Online Co-op Multiplayer mode with Ryu and Ayane - In addition to an up to eight-player multiplayer battle mode, the game now offers Ryu and Ayane as playable characters in the two-player online co-op multiplayer mode.
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- Faster, More Intense Battles - Improved enemy AI, the return of dismemberment, as well as new enemy types and battle areas have been added for greater variety in battles and increased replay value.
Service Information
Safety Warning


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1. Curiosity's First Wide Panorama
Curiosity is having a siesta of sorts this weekend while she receives a "brain transplant" of sorts that will tweak how Curiosity drives, better image processing and how its robotic arm operates. The goal with Curiosity is to get it to create its own path based on the surface of Mars, rather than relying on maps transmitted from back home. We should be receiving more and better images next week, but in the meantime NASA has released this panoramic view stitched together by thumbnails.
2. Fantastic Lego Model of the Rover
Jet Propulsion Laboratory engineer Stephen Pakbaz helped to design the Curiosity rover in real life, but he didn't stop there. He built this detailed Lego model for fun, but you can support it at the Cuusoo Project — where, if it garners enough support, it could become a new Lego set!
4. Mohawk Guy At NPR!
NPR sat down with our favorite Mohawk Guy and Adam Steltzner, the engineer in charge of Mohawk's landing, to play a little "Not My Job" and answer questions about the Mars candy company. Listen to the interview and the game here to find out who won.
- #Brexit fallout: Markets around the world fell again Monday, and analysts expect the U.K. to enter a "mild recession" by early 2017.
- The U.S. Supreme Court has ruled that abortion provider restrictions in Texas are unconstitutional.
- It's #TurnUpToVote week at BuzzFeed. Ready for November? You can register right here 🇺🇸


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Mac: Airfoil streams audio from any app to AirPort Express
Rogue Amoeba Software has announced the immediate availability of Airfoil (originally announced as Slipstream), its new Mac software that enables users to send audio from any application (instead of just iTunes) directly to an AirPort Express base station. "Everyone who owns one of Apple's popular AirPort Express devices will be thrilled to be able to send audio from applications like RealPlayer, Windows Media Player, QuickTime, and Safari right to their remote speakers," says the developer. "In just seconds, Airfoil can send audio from almost any application on OS X directly to the AirPort Express." Airfoil is available at an introductory price of $20.
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Neat—and you can try it free—but there's a lag (the nature of AirTunes) and it works with "almost any" one app at a time, not your total sound output.
So it's not necessarily the ideal universal answer for gaming, DVDs, system alerts, iChat AV, etc.
But it still sounds like a great new use for AirPort. (And they mention that some players like VLC offer a workaround to help keep audio and video in synch.)
Posted by Nagromme on March 1, 2005 at 10:41 AM (CST)
sounds cool.. I'm sure it will evolve as faster wifi standards are released. can't wait till "n" is released!
Posted by apple juice on March 1, 2005 at 10:47 AM (CST)
I use their Audio Hijack Pro, which very easily diverts the sound from any single application (as noted by Nagromme) to a recorded file. This program should work equally well.
Posted by thenightfly42 on March 1, 2005 at 11:38 AM (CST)
Awesome idea…sux that password protected AirportExpress speakers are not supported.
Posted by Xenious on March 1, 2005 at 12:30 PM (CST)
From what I read the other day, 'n' won't be available until 2007, so video transfer will be a while away. Might check this out when I get airport, so I can play online radio and films through my CD player without having to move my laptop. Anyone found where the workaround in VLC is? I couldn't see it.
Posted by GWARREN on March 1, 2005 at 1:06 PM (CST)
pre-n hardware is already out. those companies who decided not to wait on standards have already implemented the technology
Posted by whs on March 1, 2005 at 1:31 PM (CST)
This is an obvious improvement over being limited to iTunes.
Posted by dewarner on March 1, 2005 at 1:57 PM (CST)
The VLC workaround seems to be to check the Advanced box at the bottom of Preferences, and under Audio set a manual "audio deynchronization compensation value." With a little trial and error, you should get the sound in synch.
I don't have Airfoil, so I'm just guessing looking at VLC.
Posted by Nagromme on March 1, 2005 at 10:54 PM (CST)
Any chance of a windows version?
Posted by JT on March 2, 2005 at 3:10 AM (CST)
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Hillary Clinton clinches Democratic Party nomination
The former Secretary of State clinched the required number after a strong showing in Puerto Rico's Democratic primary on Sunday and additional support from super delegates.
BJP's challenge will be met democratically: Left parties
Describing BJP's demonstration as a part of its fascist functioning, Raja said the L...
Indian-American Neera Tanden appointed to Democratic party's policy panel
Indian-American Neera Tanden has been appointed by Democratic party...
US President Barack Obama is 'fired up' for Hillary Clinton as Democrats seek to unify party
Clinton, the presumptive Democratic nominee, said it "means the world" to her that Obama had her back in a bruising campaign for the Nov. 8 election.
Arrogant liberals are doing a big disservice to liberalism
Over the past two years an unhappy divide has grown, something we did not expect when the nation elected Prime Minister Modi on the promise of 'sabka saath, sabka vikas'.
Brexit: The sensational outcome is a slap in the face of liberals
Influx of job stealing migrants from eastern and southern Europe and the portrayal of Britain as a victim of the Brussels-based, power-hungry EU bureaucracy touched raw nerves.
FDI reforms in aviation: Experts hail 'liberal' move
Under the new FDI norms, foreign investors can now have up to 100 per cent stake in local carriers, while the equity holding was has remained restricted at 49 per cent.
Banks set to lobby RBI for liberal loan recast norms
To avoid another set of bad loan pile up, the central bank has said that banks could split the debt in to two for struggling companies – sustainable and unsustainable.
North East Democratic Alliance meets for Cong-free region
BJP's grouping of non-Congress parties, North East Democratic Alliance (NEDA), which was formed last month, held its first meeting in Guwahati.
As Democrats nudge Sanders, GOP tries to rein in Trump
Republican nominee, has made only transient efforts to impose discipline on his campaign and turn toward courting mainstream general election voters.
Culturally, Rajan is more Indian than many of our liberals. My objections are about his policies: S Gurumurthy
The RSS ideologue says that Indian experts, whose learning curve has been abroad, should experience India for a period like Mahatma Gandhi did.
Bernie Sanders vows to stay in Democratic nomination race
"We are going to fight hard to win the primary in Washington, DC," Sanders told supporters in California, referring to the final nomination contest next Tuesday.
Hillary Clinton claims historic victory in Democratic primary
Clinton had already secured the delegates needed for the nomination, according to an Associated Press tally. She added to her totals with victories in New Jersey and New Mexico.
Hillary Clinton has delegates to win Democratic nomination
Clinton's rise to presumptive nominee arrived nearly eight years to the day after she conceded her first White House campaign to Barack Obama.
Hillary Clinton aims to secure Democratic nomination this week
Voters in California, Montana, North Dakota, South Dakota, New Jersey and New Mexico will vote in nominating contests on Tuesday. Clinton has scheduled rallies in Los Angeles.
Bernie Sanders setting terms for closing out democratic race
Bernie Sanders is negotiating an end to his fight with Hillary Clinton and staking a claim on directing the future of the Democratic Party.
Japan's Liberal Democratic Party sets date for leadership vote, PM Shinzo Abe bound to win
Yomiuri Shimbun, Japan's major daily said that all seven factions within the party supported Shinzo Abe and there were no signs he would face a contender in the September 20 vote.
India's real elites: If the Constitution is his only holy book, liberals will stand with Narendra Modi
India's liberals are not concerned with Modi's choice of clothing or Mayawati's handbag, and in fact applaud the PM's courage at speaking in a language foreign to him.
Democrat Hillary Clinton postpones campaign rally after Orlando massacre
Clinton was scheduled to appear with President Barack Obama, who endorsed her bid for president last week.
US Democrats want 'major role' for Bernie Sanders: Poll
His challenge to Clinton, one of the best-known figures in American politics, lasted far longer than expected, as he racked up strong results in a number of state nominating contests.
Hillary Clinton would be Democratic nominee: Dianne Feinstein
Feinstein claimed that the recent report of the Inspector General of the State Department does not say that she violated the law.
Bernie Sanders under pressure to quit as Democrats look to unite
Sanders to keep his campaign alive in some form, his new found political currency into policies to change the Democratic Party, the Senate or even the country itself.
Hillary Clinton declares victory in Democratic race, hails a milestone
Clinton hailed a historical "milestone" for women Tuesday as she claimed victory over rival Bernie Sanders in the Democratic White House nomination race.
Hillary Clinton wins Puerto Rico's Democratic presidential primary
Clinton overwhelmed Sanders in Puerto Rico's Democratic presidential primary on Sunday, putting her within striking distance of capturing her party's nomination.
I will be Democratic presidential nominee: Hillary Clinton
"I will be the nominee for my party. That is already done in effect. There is no way that I won't be," Clinton told CNN in an interview.
Battling to hold off Sanders, Democrat Clinton to assail Trump on foreign policy
Republicans see weakness in Clinton's foreign policy as Obama's first-term secretary of state, given foreign policy problems such as the Syrian civil war.
NIA special court convicts Meghen chairman of militant outfit United National Liberation Front (UNLF)
Those convicted included Meghen alias Sanayaima Singh, chairman of Manipur based proscribed militant outfit, outfit United National Liberation Front (UNLF).
Japan ruling Liberal Democratic Party wins big
It won a large majority on its own, and appeared to be headed for a two-thirds majority together with its coalition partner, the Buddhist-backed Komei Party.
Progressive Democrat Front announces its own candidate for Rajya Sabha
Tamta's nomination by Congress had not gone down well with the conglomerate, says PDF stood steadily by the Congress during the recent political crisis in the state.
BJP readies Mission Northeast, forms Northeast Democratic alliance
BJP leader Himanta Biswa Sarma is made convenor of the NEDA. Political analyst view this development as preparing Himanta for a larger role.
Is Aam Aadmi Party losing steam in Punjab?
The AAP leadership in the state is now grappling with problems like finding a face to project as its chief ministerial candidate.
Aam Aadmi Party suspends Alka Lamba as party spokesperson
Reacting to her suspension, Lamba said that she is a disciplined worker and will abide by the party's decision.
Aam Aadmi Party suspends Alka Lamba as party spokesperson
Aam Aadmi Party has suspended MLA Alka Lamba from the post of party spokesperson for two months.
DoT against liberal payment terms
At this base price, each block would fetch the government overRs 57,000 crore, which is more than half the entire proceeds of the 2015 sale of spectrum.
SP most liberal party, you can take a selfie with me: Akhilesh Yadav
In an apparent reference to Prime Minister Narendra Modi, Akhilesh also took a dig at him saying the BJP has spread a new disease of clicking selfies.
Five states get 428 crorepati MLAs: Association for Democratic Reforms
In 2011, Assam had 39 per cent of crorepati MLAs, while Kerala had 25 per cent, Puducherry (63 per cent), Tamil Nadu (51 per cent) and West Bengal (15 per cent).
Rahul Gandhi holidays abroad, party crumbles in Karnataka
Congress walks a tightrope in Karnataka after a major reshuffle in the Siddaramaiah led state government, that culminated in protests on the streets by disgruntled leaders.
Samajwadi Party revokes merger with Mukhtar Ansari's party, Akhilesh Yadav has his way
The party also decided on reinduction of Secondary Education Minister Balram Yadav, who was sacked by Akhilesh apparently for facilitating the merger.
Government formation wait continues in Jammu and Kashmir; People Democratic Party hints alliance with BJP on
BJP has maintained that they have no problems with PDP and were waiting for alliance partner's decision on government formation.
Political parties observe 'Telangana Liberation Day'
Parties hoisted the tricolour at their offices to observe the day as 'Telangana Liberation Day' marking the merger of Hyderabad state with the Indian Union.
Donald Trump aide dismisses audio tape as Democrats raise character issue
A top aide to Donald Trump said on Sunday he did not believe the Republican presidential front-runner posed as his own spokesman to brag about his personal life.
Fearing Donald Trump, some Democrats up pressure on Bernie Sanders to exit
Sanders soldiers on, frequently telling the thousands of supporters who attend his rallies that he still has a narrow path to the nomination.
Parties in Karnataka vie for seats
The legislative assembly will elect four RS and seven Legislative Council members and the existing strength of political parties will ensure victory of their candidates
IRNSS: Desi GPS to liberate India from dependence on US, Russia
The scientists who were till then glued to their monitors burst into cheers. India's mini GPS, a regional positioning system, was ready to be rolled out.
Whole world knows which party is responsible for parliamentary logjam: PM Modi
In an exclusive interview with Times Now, Prime Minister Narendra Modi said that there is one party which has problems and the whole world knows that party.
UK's Labour party leader defies revolt over Brexit strategy
Eleven members of the veteran socialist's top team resigned, blaming him for failing to rally the party's core working-class vote base to support the "Remain" campaign.
US President Barack Obama's loss may be political win for Democratic Party
Latinos are a crucial constituency in states including Florida, Nevada and Colorado that are battlegrounds in the presidential election.
Hillary Clinton wins New York Democratic primary
Hillary Clinton won her home-state New York primary, shutting down one of the few remaining chances for rival Bernie Sanders to change the direction of their Democratic presidential contest and putting the front-runner on a likely irreversible path to the nomination.


text:
Call for checks on stadium engineer's other work
Relevant offers
An institute is calling on people who had work undertaken by a disgraced city design engineer to get it checked.
Institution of Professional Engineers New Zealand (IPENZ) this week announced it had expelled Anthony Stanley Major, of Invercargill, as a member because he breached their code of ethics.
Major designed and observed the construction of the original Stadium Southland, which collapsed in 2010 after a snowstorm.
Yesterday, IPENZ president Kevin Thompson said the institution encouraged anyone who had work done by Major to get it checked.
IPENZ believed Major had confined his work to Southland and Otago, but was unaware of how many structures he had worked on.
Councils had approved building consents for the structures on the basis they were designed by a qualified engineer, and this was an issue that needed to be looked at, Thompson said.
A spokesman for Major said he was a registered engineer and had met IPENZ's qualification standards to be a member but declined to comment further.
On Tuesday, the spokesman said Major was disappointed with the IPENZ disciplinary committee's decision and felt it was not a fair reflection of his culpability in this matter.
The Invercargill City Council released a statement saying it was now concerned about buildings in the city. Council chief executive Richard King said he had asked IPENZ for advice on the actions that needed to be carried out on work undertaken by Major.
In the meantime, if building owners believed there was an immediate safety issue they should contact an engineer and inform the council's building section, King said.
Council staff were now searching property records to identify projects Major had been involved with.
Once the council had received more information from IPENZ, it would communicate further with the community as to what steps building owners should take, he said.
Yesterday, IPENZ released the written decision of the disciplinary committee.
Its investigation began in July 2012 after a Building and Housing Department report into the collapse of Stadium Southland was completed.
"At the hearing he still failed to take responsibility, contradicted his earlier written submissions and tried to lay the blame on anyone other than himself," the decision says.
In describing his previous experience, Major told the committee about the Farmers Buildings and a shopping arcade.
But under questioning he said he did not feel that there was any different duty of care for public buildings over any other, the decision says.
Major had to pay IPENZ costs of $10,720.
- The Southland Times
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Star Tracks
The new parents root for Cruise's son Connor in Beverly Hills. Plus: Jennifer Garner, Halle Berry and more
Credit: Carmen Valdes / Photo Image Press
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App of the day: Tiny Thief review (Android)
If you were one of those Daily Mail sorts, you'd have a fit about the current "morality" of both gaming and TV. On TV, for example, you've got serial killers as heroes, motorcycle outlaws as role models and drug dealers as father figures. Now, in its latest, Rovio wants us to control a Tiny Thief as he goes about his nicking.
Tiny Thief is at least, supposed to be a hero in a world of corruption. Although this neither matters nor is especially clear from the narrative, which very much follows the standard Rovio model of giving you a splash screen with some explanatory drawings on it. It's simple, effective and must cut down on its translation costs.
Tiny Thief
- Format
- Android and iPhone
- Price
- £1.99
- Where
- Google Play and iTunes
The first thing we loved about Tiny Thief is the visual style. We played on Android, but there is an iOS version too. We can't help but think the game would look good on a bigger, higher-resolution screen than our Galaxy Note II's 720p. The style is very cartoon-like, and there are lots of bright colours, which we always find very soothing.
Moving around is simple enough: you tap where you want to go, and your tiny criminal moves to where you tap. Moving up ladders is achieved after a prompt appears on screen, and there are other things you can interact with. For example, sometimes you might need to hide in a barrel, or move something around to get rid of a guard.
Each level has one objective - at least early on, we haven't finished the game yet - namely, to steal something. This might be a bit of cheese, or it might be some "wanted" posters, featuring your very own compact kleptomaniac. There are also sub missions, like cooking something. There are also squirrels to find and release. Early on, these are obvious; later, they're more concealed, but you just tap where you think they are to set them free.
The early tutorial is very clear and helpful. This gets you into the swing, with loads of tips about where to press. It releases you into the game gently, letting your own ingenuity take over reasonably quickly. It's quite a good way of teaching you the game.
Our main criticism of Tiny Thief is probably that at £1.99 it feels a bit expensive. We'd say this game is more akin to Disney's "Where's My..." series, and its £0.70 price range, but it's still not a big sack of money. Other than that, the game is fun and we'll likely be enjoying it for some time to come.
While Tiny Thief might be on the wrong side of the law, we still love him. But then, we also love crystal meth dealers, members of motorcycle gangs, Miami-based murderers, so what do we know.
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text:
This page contains Mario Kart 7 Hints for 3DS called "Temporarily boost" and has been posted or updated on Jun 17, 2012 by Terminator 5000.
- Developer: Retro Studios
- Publisher: Nintendo
- Genre: Racing
- Release: Dec 4, 2011
- ESRB: Everyone
Temporarily boost
Its annoying when you fall off the course is'nt it well when Lakuti puts you back on track as you touch the ground touch the acceleration button ( A or Y ) to get a temporarily boost.
On Wuhu Island Loop on the 3rd Section near the gliding point go on the grass and go straight across the boost and into the sea. You should end up in front of the finish line. ( This has been patched on online play, it still works on local and singal play.)
Thats it from me Terminator 5000 today! Expect to hear community news soon OOSH!!!
Please Submit a Problem for any incomplete, non-working or fake cheat listed above.
If you know other secrets, hints, glitches or level guides, then please Submit your Cheats and share your insights with other gamers.
Stuck in this game? Set a specific, clearly defined question and let other gamers answer your question or view answers to previously asked questions. If you think you are an expert then please try to help others with their questions.
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Posted on: 01-25-2016
From: Rosalina14
Posted on: 01-06-2015
From: Ubscal99
Posted on: 08-23-2014
From: Mieto Marrio!
Posted on: 01-19-2014
From: gollum969youtub
Posted on: 06-17-2013
From: falchion
Posted on: 05-11-2013
From: kobster
Posted on: 04-19-2013
Mario Kart 7 - Trailer
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- Aug 21, 2014, Views: 186
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- Aug 21, 2014, Views: 128
Mario Kart 7 - Overview Trailer
- Aug 21, 2014, Views: 135
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text:
Photo Special10 Best Celeb Quotes This Week
The Wolf of Wall Street's Margot Robbie reveals why her sweaty love scenes with Leonardo DiCaprio weren't so hot. Plus, more from President Barack Obama, Olivia Wilde and other stars
Credit: Michael Becker/FOX
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Erin Foster and Sara Foster Out and About in NYC
- Erin Foster, Sara Foster
Daddy Yankee Performs at Chicago Mega Fest featuring Latino Artists
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Beginner Salsa (NY Style)
7pm - 8:30pm
Level 2 Salsa (NY style)
8:30pm - 9pm
Where: Dance Etc., 15757 Coit Rd, Dallas
We are back to our regular 1.5hour format! Join us for a beginner salsa class at 7-8p:30m at Dance Etc (15757 Coit Rd, at the corner of Arapaho, Dallas.) You can sign up for the whole 4 weeks for $75 or drop in one class at a time for $20. Don't worry if you miss the first class, we'll catch you up for the second! Come as an absolute beginner, repeat the beginner course, or move up to level 2 at 8:30pm.
RSVP here or just sign up at the door. I can't wait to meet new faces! You don't need a partner, just come as you are. (Note, we can't take cc at the door, so please bring cash.)
We also have specials... like sign up for any second course (concurrently) for $25 more (for 4 weeks)! Are you a student or school teacher with a current ID? Take the course for $60! Are you bringing a partner with you? Join the course for $130.
See you on the dance floor! Call me with questions![masked]
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BradsDirty30 by Denise Clarke - 8 days ago
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Morristown Used Mobile Homes For Sale
1. Morristown House & Mobile Home
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Bluff City, TN 37618
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149 Livingston RdBean Station, TN 37708
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5570 E Andrew Johnson HwyRussellville, TN 37860
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1210 State StWhite Pine, TN 37890
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3429 Beth Carr RdWhite Pine, TN 37890
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680 Obe Kirk LoopMohawk, TN 37810
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15. Norris Mobile Homes Doublewide
1160 Highway 11WBean Station, TN 37708
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982 Wheeler RdWhite Pine, TN 37890
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From Business: Lease to own on some homes. Lots available for lease. Affordable rates.
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From Business: Woods Mobile Homes in Sweetwater, TN has been a family owned and operated dealership since 1973. We are located in Monroe County of the beautiful East Tennessee and…
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...I am certified and licensed to teach mathematics in Massachusetts, where I previously lived, as well as run a mathematics department. I'm very confident in my skills to tutor students and have been tutoring math and SAT prep for many years. Contact me if you (or your student): - are struggling...
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Great Air fares between USA/Canada and Colombo
Wed, Jan 11, 2012, 10:20 pm SL Time, ColomboPage News Desk, Sri Lanka.
Jan 11 (NJ-USA), Sri Lanka's national carrier, SriLankan Airlines has introduced attractive, special fares, from USA and Canada to Colombo. These fares offered in combination with Air Canada, American Airlines, Virgin Atlantic Airlines and US Airways, are valid from January to March.
Sri Lanka, nestling at the tip of the Indian subcontinent, is an island of vast bio diversity, history, culture and cuisine. Often called the Paradise Island its 65,610 square kilo meters mass of land, is a journey of discovery for visitors offering everything from pristine beaches, turquoise ocean, to lush green hills in the central country dominated by tea plantations, wild life parks, heritage sites, bargain shopping and a tropical mix of spicy cuisine.
Sri Lanka, once called the "finest island of its size in all the world" by Marco Polo, offers its visitors an amazing travel experience, enhanced with warmth and hospitality inherent in its people, which is the hallmark of service on board SriLankan Airlines.
The country's natural water ways, from its cascading waterfalls, to its natural lakes and rivers, are popular for rafting, canoeing and a surf point in the south east of the country, called Arugam Bay, is rated one of the top ten surfing destinations in the world, drawing many competitors from North America to participate in international surfing events organised by SriLankan Airlines.
The airline has also used its abundant water ways to operate domestic flights to many parts of the country. SriLankan Air Taxi, which only lands on water, takes passengers to any part of the country, within an hour which provides a panoramic view of the breath taking landscape of the country.
National Geographic Traveller magazine recently rated Sri Lanka among its six best destinations to travel in 2012.
SriLankan Airlines has partnerships with several US and Canadian carriers, to ensure seamless connections, across continents, the most recent code share agreement being with Air Canada, in October last year.
The airline operates wide-body aircraft with luxurious interiors, flat bed seating, and state of the art audio video on demand (AVOD) entertainment systems, to all its long haul destinations.
SriLankan Airlines operates daily to London and is the only carrier to have a non-stop service to Colombo from London.
SriLankan flies to 60 destinations in 33 countries.
For reservations, Passengers and agents can contact the nearest SriLankan Airlines office or Travel agency. Passengers can also book seats on: www.srilankan.com
For more details please call SriLankan Airlines - USA on 732 205 0017 or visit www.srilankanusa.com
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Mother's Day
Moms' Best Moves
These ladies have skills! From Denise Richards to Heidi Klum, see the signature ways these moms juggle playtime
Credit: Lawrence Schwartzwald/Splash News Online (2)
Updated: Friday May 11, 2007 | 06:00 PM EDT
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Secrets of Success
Good sunday morning run and faulty scale
Sunday, May 12, 2013
I planned a 10K today, but I decided to add some distance, because weather is ideal today for running : 14 oC / 57 oF, overcast and windy.
So on the way back, I added another 1.5 mile segment on the other side of the hill (steep and very narrow and bumpy trail).
I found the right comfortable pace, that allowed me to breath slowly and deeply all the way,
so I finished 7.5 miles at 11:23 min/mile.
I felt wonderful during the entire run, and the bonus: I saw a fox!
This is the first time in my life I saw a free fox alive!
It was coming up on the hillside, and probably couldn't smell and hear me because of the wind, and it was quite surprised when saw me.
Something must have been wrong with the scale in the past few weeks, because now it shows about 2-3 pounds less for everybody.
Now I "calibrated" the scale, measuring 8 kg of weights and it is correct now.
So, my high weight was not so high after all : )
I suspected this, because my weight was up about 5 pounds, and my waist-hip-thigh measurements hardly changed at all.
But the false alarm helped me develop good dinner eating habits so it was a good thing : )
PS: oh, I forgot to mention the change happened after replacing the batteries, so that's in line with what ARCHIMEDESII wrote!
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My SparkPage
The fox must have been amazing! I envy you!
For the scale:
I had a similar issue with my bathroom scale. I didn't know it's not correct anymore. However, when I bought my Omron scale about 2 years ago, they didn't show the same values, always 1-2 kg difference. Then I checked both scales in the factory where I was working, they had official Mettler Toledo big scale used to weigh raw materials. Then I could verify how precise they are.
I have the impression that many of the scale out there are just simply not showing the right values. But if they show consistent error, they can still help in tracking changes.
1140 days ago
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My SparkPage
Yay! Good run! You've got a GREAT pace! Happy for your good news about the scale! Hope you had a good Monday! I opted to soak in some sunshine during my lunchtime today. Gotta love Vitamin D! Keep up the good work! Thanks for the encouragement! Spark on!
1144 days ago
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My SparkPage
Nice run! It is always fun to see wildlife. We regularly see foxes and a large herd of turkeys. Now that it is warmer, we have seen 4 snakes recently too.
Keep up the Spark!
1144 days ago
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My SparkPage
I love those kind of runs! They are always the best.
1145 days ago
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My SparkPage
Foxes are shy, and they hide from humans pretty well. I occasionally see a fox, typically crossing a road I'm driving on. Why does the fox cross the road? I dunno, maybe it's looking for a chicken.
1145 days ago
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My SparkPage
Congrats on your run.
1145 days ago
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Sounds like quite an interesting, enjoyable run! I'm sorry your scale's not working well. That's so frustrating.
1145 days ago
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My SparkPage
We have foxes (and coyotes) in our neighborhood. And I live in town (Austin). I have seen coyotes in the ravine behind my house. And once while I was running on a neighborhood street, a fox emerged from the underbrush on the hill next to me and ran across the road right in front of me. I'm not sure which of us was more surprised.
My cats are definitely indoor cats. It's not safe out there and not just because of cars. In fact my neighbor saw a coyote tree a cat on our street. The coyote was back the next day looking for him. He (the cat) is a wily old devil. I'm not sure how he has survived this long running free.
1145 days ago
Comment edited on: 5/12/2013 11:33:06 AM
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My SparkPage
Always a good idea to take advantage of good weather, and to run if you feel like it!
1145 days ago
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so funny........about the scale. We have one of those old doctor's scales that have the weights you move on them. They refuse to lie.
We have foxes or at least one fox in the neighborhood. It seems to take a regular route and I've seen it go down my neighbor's driveway at least 3 times. My dogs always go nuts when they see/smell him. What a lovely sight!
Yesterday was a 2 deer, 3 bunny day for me!
Happy Sparking!
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Doing more than you planned, weighing less than you thought, seeing your first fox in the wild- Maybe good things do come in threes.
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Well done!
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Great run! I'd love to see a live fox, we have plenty of deer, though. Sounds like the both of you were plenty surprised! Glad the scale got ironed out, too. Hope you have a great day!
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You might also want to check the batteries. If your scale has been acting a little wonky recently, it might need new batteries.
It's really nice here too. I'm planning on taking a short walk in a little while. I need to make a run to the grocery store.
Very cool that you got to see a fox. I've never seen one. Although, I have seen cotton tail bunnies. I've seen wild turkeys too. I've seen them a bunch of times. They seem to enjoy living in the city, go figure. LOL !
1145 days ago
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|Showing 1-20
Vijay Hazare Trophy 2009-10, 2nd round
Karnataka and Tamil Nadu make it two in two
Feb 11, 2010: A round-up of the action from the second round of matches in the 2009-10 Vijay Hazare Trophy
Ranji Trophy Super League, 6th round, 4th day
Delhi keep quarters hopes alive
Dec 11, 2009: A round-up of the fourth day's action from the sixth round of the Ranji Trophy Super League
Ranji Trophy Super League round-up, 2nd round, 1st day
Vinay Kumar hands Karnataka the advantage
Nov 10, 2009: A round-up of the first day's play of the second-round matches of the Ranji Trophy Super League 2009-10
Chandigarh Lions v Delhi Giants, ICL, Panchkula
Delhi pip Chandigarh via bowl-out
Nov 5, 2008: Delhi Giants beat Chandigarh Lions 2-0 in a bowl-out in a high-scoring ICL encounter in Panchkula where both teams scored 200
Chandigarh Lions v Royal Bengal Tigers, ICL, Gurgaon
Oct 28, 2008: A sparkling all-round performance from TP Singh ensured two crucial points for Chandigarh Lions against Royal Bengal Tigers
India XI v World XI, Indian Cricket League, Hyderabad
Martyn eases World XI to victory
Apr 9, 2008: Damien Martyn steered the World XI to a comfortable eight-wicket win in the inaugural match of the fourth and latest tournament of the Indian Cricket League
Chandigarh Lions v Kolkata Tigers, ICL, Gurgaon
Jhunjhunwala and Nechim power Tigers' win
Mar 26, 2008: The Lions stumbled for the second consecutive day in a run-chase of 160-odd as they fell to a 22-run loss against the Kolkata Tigers
Chandigarh Lions v Delhi Giants, 6th match, ICL
TP Singh's all-round show hands Lions win
Mar 15, 2008: Chandigarh Lions posted an easy 34-run win on the back of Tejinder Pal Singh's brisk 38 and two wickets in an over which snuffed out Delhi Giants' challenge in Panchkula
Chandigarh Lions v Chennai Superstars, 9th match
Harvey shines in Superstars win
Dec 8, 2007: Ian Harvey played anchor for nearly the entire innings as Chennai Superstars chased down 147 with one ball to spare
Orissa v Railways, Group A, Twenty20, Mumbai
TP Singh spurs Railways to easy win
Apr 17, 2007: Orissa and Railways are two teams unlikely to attract crowds at the best of times
Ranji One-Day Trophy, 2006-07
Sharma stars in thrilling win for Rajasthan
Feb 18, 2007: A round-up of the ninth day in the 2007 Ranji One-Day Trophy
Ranji Trophy Plate League, Semi-finals, 1st day
Vishal Bhatia derails Railways
Jan 10, 2007: Round-up of the first day of the Plate Group semi-finals in the 2006-07 Ranji Trophy
Ranji Trophy Plate League, 3rd round, 1st day
Tejinder and Khanolkar put Railways in command
Dec 10, 2006: A round-up of the first day's play of the third round Ranji Trophy Plate Division matches
ICC Champions Trophy, 2006
'I was expecting the call-up' - Rohit Sharma
Aug 9, 2006: Rohit Sharma and Tejinder Pal Singh talk about their selection and their dreams for the future
ICC Champions Trophy, 2006
Ganguly included in probables list
Aug 9, 2006: Sourav Ganguly and Zaheer Khan have been included in the Indian probables list for the ICC Champions Trophy in October. Anil Kumble and VVS Laxman have also been included
Australia A v India A, Top End Series, Darwin
Spinners star as India A win again
Jul 8, 2006: Led by their spinners, India A recorded their second win of the Top End Series, easing past Australia A by four wickets in a low-scoring match at Darwin
India A v NTCM XI, Four-day match, Darwin
Tejinder Pal Singh stars in easy win
Jul 2, 2006: India A completed a10-wicket win over Northern Territory Chief Minister's XI in their four-day match at the Marrara Cricket Ground in Darwin
Ranji Trophy one-day tournament, 2005-06
Railways and UP set up title clash
Mar 19, 2006: A round-up of the semi-final matches that took place on March 19
Deodhar Trophy 2005-06
Yusuf Pathan five-for seals West Zone win
Feb 26, 2006: A round-up of the Deodhar Trophy matches that took place on February 25
Ranji Trophy One-Day Tournament, 2005-06
Winning starts for Bengal and Jharkhand
Feb 12, 2006: A summary of the latest action in the Ranji Trophy One-Day Tournament
|Showing 1-20
How much is a century against Zimbabwe worth? (56)
Cricket stats need to take into account various contextual factors relating to players' and teams' performances if they are to be meaningful
'The dream is to come back and play for three to four years' (39)
Mohammad Asif is playing club cricket in Scandinavia as he strives for a Pakistan comeback and to rebuild his career in the wake of the spot-fixing scandal
What's the verdict on the pink ball in Indian conditions? (27)
Visibility is good, so is durability, and while it does swing a fair amount, it ought to spin as well
Give the World Test Championship a chance (27)
Test cricket needs to be given back to the people. Everybody must buy in to this bigger picture or the moment will pass us by
'I don't mind losing a few on the way to a win' (25)
Angelo Mathews talks about the challenges of leading an inexperienced team, and the possibility of giving up the T20 captaincy


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Dawson's Creek 1998 | TV Show Watchlist
Season 6, Episode 21 Goodbye, Yellow Brick Road
First Aired: April 30, 2003
Pacey steels himself to deliver bad investment news to an unsuspecting Dawson, who has re-created his high-school bedroom and maxed out three credit cards on equipment for his film. Also: Grams gets an unexpected visit from her daughter; and Joey returns to Capeside and reconnects with Dawson. Jack Osbourne appears as himself.
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- Premiered: January 20, 1998
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by Joe Romm Jun 23, 2016
China's government has put forth dietary guidelines to cut meat consumption in half. They have enlisted global celebrities like Arnold Schwarzenegger, to explain that eating less beef is good for your health AND for the climate.
by Alejandro Davila Fragoso Jun 23, 2016
Cities may be responsible for 75 percent of global CO2 emissions, but mayors are taking action.
by Natasha Geiling Jun 23, 2016
A pipeline in southern California has spilled 700 barrels of crude oil as of Thursday morning.
On June 3, a Union Pacific oil train derailed in Oregon, spilling thousands of gallons of oil and forcing the evacuation of a small town. This week, Union Pacific will resume shipping oil.
by Marlene Cimons -- Guest Contributor Jun 23, 2016
"This is unprecedented. No other country has tried to do what India is trying to do -- build a clean energy economy and bring millions of people out of poverty."
by Samantha Page Jun 22, 2016
Britain's exit could actually spell good things for environmental safety in the E.U.
by Sydney Pereira Jun 22, 2016
The regulations were a step forward in the effort to protect communities who live near oil and gas wells.
by Alejandro Davila Fragoso Jun 22, 2016
Tesla announced it wants to buy SolarCity, one of the largest solar energy companies in the world.
by Natasha Geiling Jun 22, 2016
There are more than 6,500 concentrated animal feeding operations in North Carolina, which means a lot of animal feces finds its way into the air and water.
by Katie Valentine Jun 21, 2016
The worst bleaching event in recorded history isn't likely to let up anytime soon.


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Tue., April 14, 2015 2:50pm
Georgia Officials Approve Tuition Increases At State Schools
Tuition is going up for Georgia students the state's 30 colleges and universities. The Georgia University System's board approved the rates at a meeting Tuesday. In-state students at Georgia Tech will see the biggest increase, up $405 per semester. That's a 9 percent increase.
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A study by the University of Georgia found the 35 schools in the University system generated $13.2 billion for Georgia's economy last year. That's mainly due to the growing number of students and the amount they spend..


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Identification and characterisation of a novel adhesin Ifp in
In order to identify new virulence determinants in
We termed YPTB1572 Ifp (Intimin family protein) and show that it is able to bind directly to human HEp-2 epithelial cells. Cysteine and tryptophan residues in the C-terminal region of intimin that are essential for function in EPEC and EHEC are conserved in Ifp. Protein binding occurred at distinct foci on the HEp-2 cell surface and can be disrupted by mutation of a single cysteine residue at the C-terminus of the protein. Temporal expression analysis using
A new
Within the genus
Pseudogenes in
In the same family of bacterial adhesion proteins as invasin, is intimin, an important adhesin expressed by enteropathogenic (EPEC) and enterohaemorrhagic (EHEC)
There is considerable sequence variation between the intimins from different
Analysis of
Strains used and culture conditions
Table 1
The strains and plasmids used in this study
Strain or plasmid
This study
This study
This study
This study
This study
This study
This study
This study
Construction of
PCR primers (Table
Table 2
Primers used in this study
Restriction sites are in bold and position of mutated cysteine to glycine residue is underlined.
Bioluminescence and optical density of Lux constructs
Bioluminescence and optical density were monitored simultaneously in 96-well microtitre plates using the Anthos Lucy1 combined photometer and luminometer controlled by the Stingray software (Dazdaq). Overnight 30°C cultures of
Construction of Maltose Binding Protein (MBP) fusion proteins
Primers YptbIntMBP-1 and YptbIntMBP-2 (Table
Purification of MBP-Ifp and MBP-IfpC337G
Analysis of MBP-fusion protein binding to HEp-2 cells by fluorescence microscopy
Binding of MBP-tagged Ifp was determined by fluorescence microscopy as described previously
FACScan analysis of MBP-fusion protein binding to HEp-2 cells
A similar methodology was used as for the fluorescence microscopy as described previously
Analysis of co-localisation of MBP-fusion protein and the receptors CD59 and β1 integrin on HEp-2 cells by fluorescence microscopy
A similar methodology was used as for the fluorescence microscopy described above with the following modifications. After the MBP-fusion protein incubation the cells were washed 5 times with PBS then incubated with 250 μl of a 1:20 dilution rabbit anti-Ifp (CovalAb, this study) and 1:1000 dilution of mouse anti-CD59 (Invitrogen) or a 1:1000 dilution of mouse anti-β1 integrin in binding solution for 30 minutes at 37°C/5% CO2. The cells were washed 5 times with 1 ml PBS then incubated with 250 μl of a 1:1000 dilution of goat anti-rabbit IgG Alexafluor 594 (Invitrogen) and 1:1000 dilution of goat anti-mouse IgG Alexafluor 488 (Invitrogen) for 30 minutes at 37°C/5% CO2. The cells were washed 5 times with 1 ml PBS then fixed for 30 minutes at 4°C with 250 μl 2% paraformaldehyde (w/v). The coverslips were removed from the wells, washed with PBS then mounted onto glass slides with Vectashield-DAPI mounting medium (Vector Laboratories). The slides were examined using an Axiovert 200 M confocal microscope (Zeiss). At least three areas of approximately 10 cells each were examined per sample and the experiment was performed on three independent occasions.
Construction of
Construction of
Plasmid cured strains
Wild type, defined mutants and
Adhesion and invasion of HEp-2 cells
HEp-2 cells were cultured overnight at 37°C 5% CO2 on coverslips in 24-well plates at 2 × 105 cells/well in 1 ml tissue culture medium. The 10 ml LB broth cultures of IP32953 wild type (IPWT), defined mutants (IPΔIFP, IPΔINV, IPΔIFPΔINV) and mutant with complemented
Detection of the presence of invasin by western blot
Strains were cultured for 15 hours at 28°C and 37°C, the OD600 nm measured and cultures adjusted so all strains had equal quantities of bacteria/ml. Strains were run on a SDS-PAGE 12% Bis-Tris gel, blotted onto nitrocellulose membrane and blocked with 5% milk-PBS-0.1% Tween20. Invasin was visualised by staining with anti-invasin monoclonal antibody
Identification of an intimin and invasin-like protein in
The genome sequence of
Additional file 1
Amino acid alignment of Ifp from the four currently sequenced genomes of
Click here for file
Figure 1
Amino acid alignment of intimin, ifp and invasion
Amino acid alignment of intimin, ifp and invasion. The C-terminal 280 residues of EPEC 2348/69 intimin (Int280) and the corresponding regions from
Thermoregulated temporal expression of
The expression of
Figure 2
Temporal expression of
Temporal expression of
Additional file 2
Growth curves from the temporal expression of
Click here for file
Ifp binds to localised foci on HEp-2 cells
Invasin and intimin are able to bind directly to specific receptors on the surface of mammalian cells. We therefore investigated the ability of Ifp to bind directly to HEp-2 cells using a MBP tagged Ifp purified protein (MBP-Ifp). In addition, to determine if the terminal cysteine was as important in Ifp functionality (as it is in invasin and intimin), a MBP-Ifp recombinant protein with the terminal cysteine mutated to a glycine (MBP-IfpC337G) was constructed and tested. Utilising flow cytometry, FACScan analysis showed a shift in the peak of fluorescence of HEp-2 cells which had been incubated with MBP-Ifp (Figure
Figure 3
FACScan analysis of the binding of purified MBP-fusion proteins to HEp-2 cells
FACScan analysis of the binding of purified MBP-fusion proteins to HEp-2 cells. Cells were incubated with (A) MBP-Ifp, (B) MBP-IfpC337G, (C) MBP or (D) PBS and binding was visualised with anti-MBP and anti-rabbit Alexafluor 488 antibodies.
Figure 4
Binding of purified MBP-fusion proteins to HEp-2 cells
Binding of purified MBP-fusion proteins to HEp-2 cells. Cells were incubated with (A) MBP-Ifp, (B) MBP-IfpC337G, (C) MBP or (D) PBS and binding was visualised with anti-MBP and anti-rabbit Alexafluor 488 antibodies. Representative cells are shown and the 10 μm ruler is shown in red.
Interestingly, this binding appears to be localised to specific foci on the cell surface, rather than a random scattering of fluorescence across the entire cell surface. This suggests that the protein is binding to specific receptors on the cell surface which are localised in foci. In order to investigate if a putative receptor was localised in cholesterol and sphingolipid-enriched plasma membrane micro-domains (lipid rafts), we used co-localisation assays. In this instance the GPI-anchored protein CD59, which is known to localise to these microdomains
Figure 5
Fluorescence microscopy showing co-localisation of (A) CD59 and (B) β1 integrin with purified MBP-fusion proteins on HEp-2 cells
Fluorescence microscopy showing co-localisation of (A) CD59 and (B) β1 integrin with purified MBP-fusion proteins on HEp-2 cells. Cells were incubated with MBP-Ifp or MBP-IfpC337G. MBP-fusion proteins were visualised with anti-Ifp and anti-rabbit Alexafluor 594 antibodies. CD59 was visualised with anti-CD59 and anti-mouse Alexafluor 488 antibodies. β1 integrin was visualised with anti-β1 integrin and anti-mouse Alexafluor 488 antibodies. Representative cells are shown.
Adhesion and invasion assays
In order to confirm the role of Ifp as an adhesin, we constructed an insertion mutant in the
Figure 6
Adhesion and invasion of HEp-2 cells by wild type IP32953 and defined mutants
Adhesion and invasion of HEp-2 cells by wild type IP32953 and defined mutants. (A) adhesion, (B) invasion (C) adhesion with pYV cured strains, using differential staining assay. Wild type (IPWT) was compared to insertional mutants of
Figure 7
Survival of
Survival of
In this study we investigated the role of a novel
The C-terminal 280 amino acids of intimin are the functional domain in this adhesin
We demonstrated direct binding of Ifp using both flow cytometry and fluorescence microscopy, where the MBP-Ifp fusion proteins could bind to HEp-2 cells. By contrast the MBP alone did not bind and the specific cysteine residue mutant MBP-IfpC337G showed greatly reduced levels of binding (Figures
The invasion and adhesion assays confirmed the microscopy and flow cytometry results, in demonstrating a role for Ifp as an adhesin, as the levels of adhesion were reduced with IPΔIFP in comparison to wild type (Figure
We have shown the presence of a novel functional adhesin in
Authors' contributions
All authors read and approved the manuscript and contributed to experimental design. PS and BW contributed to manuscript preparation.
We are grateful to G. Frankel, Imperial College, London, UK for the intimin advice; E. Carniel, Institut Pasteur, Paris, France for the
Insights into the evolution of
Application of DNA microarrays to study the evolutionary genomics of
Pathoadaptive mutations: gene loss and variation in bacterial pathogens
Pathoadaptive mutation that mediates adherence of shiga toxin-producing
Pathoadaptive mutations that enhance virulence: genetic organization of the cadA regions of
Experimental evidence for negative selection in the evolution of a
Loss of a biofilm-inhibiting glycosyl hydrolase during the emergence of
Increased virulence of
A single genetic locus encoded by
Multiple β1 chain integrins are receptors for invasin, a protein that promotes bacterial penetration into mammalian cells
M-cell surface β1 integrin expression and invasin-mediated targeting of
Crystal structure of invasin: a bacterial integrin-binding protein
Identification of the integrin binding domain of the
Characterization of the C-terminal domains of intimin-like proteins of enteropathogenic and enterohemorrhagic
Structure of the cell-adhesion fragment of intimin from enteropathogenic
The eae gene of enteropathogenic
EspE, a novel secreted protein of attaching and effacing bacteria, is directly translocated into infected host cells, where it appears as a tyrosine-phosphorylated 90 kDa protein
Actin accumulation at sites of bacterial adhesion to tissue culture cells: basis of a new diagnostic test for enteropathogenic and enterohemorrhagic
The established intimin receptor Tir and the putative eucaryotic intimin receptors nucleolin and β1 integrin localize at or near the site of enterohemorrhagic
The cell-binding domain of intimin from enteropathogenic
Intimin types α, β, and γ bind to nucleolin with equivalent affinity but lower avidity than to the translocated intimin receptor
Mutagenesis of conserved tryptophan residues within the receptor-binding domain of intimin: influence on binding activity and virulence
Molecular characterization of a carboxy-terminal eukaryotic-cell-binding domain of intimin from enteropathogenic
A 76-amino acid disulfide loop in the
Structural basis for recognition of the translocated intimin receptor (Tir) by intimin from enteropathogenic
Detection of pathogenic
Functional interplay between the
Identification of inducers of the
Tight regulation, modulation, and high-level expression by vectors containing the arabinose pBAD promoter
A region of the
Genome sequence of
A general method applicable to the search for similarities in the amino acid sequence of two proteins
Analysis of expression and thermoregulation of the
Lipid rafts: elusive or illusive?
GFP plasmid-induced defects in
Development of
Recognizing the pseudogenes in bacterial genomes
Complete genome sequence of
Invasin production by
Bacterial pathogenomics
Functional rafts in cell membranes
Clustering transfers the translocated
Survival of FimH-expressing enterobacteria in macrophages relies on glycolipid traffic
Cholesterol binding by the bacterial type III translocon is essential for virulence effector delivery into mammalian cells
The YadA protein of
YadA, the multifaceted
Effect of pre-incubation temperature on susceptibility of


text:
Summer Soundstream: Justin Jackson Performs at The Troubadour
Wadada Leo Smith In Concert at The Hammer
[Video] The Animated Woman Symposium: How Do I Look?
Video: Gina Maune's film asks one question of the viewer. The Animated Woman: A Symposium on Gender Bias in Animation was held at CalArts in Dec. 2015. Students from The…Continue reading »
CalArts President Steven D. Lavine Joins LARB Board
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EventJune 21, 2016
CNP's Shelter at The Kennedy Center
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EventJune 9 - July 3, 2016
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EventJune 22 - 26, 2016
Exit Interview: CalArts 2016 MFA Art Graduates Show at Human Resources LA
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MARKET REPORT: Private jet management firm Hangar 8 soars to record high
Fans of that irritating TV series The X Factor will remember the opening episode of the current series when Gary Barlow, Nicole Scherzinger, Louis Walsh and Tulisa Contostavlos exited a jet aircraft on their way to an amphitheatre to judge the latest bunch of wannabee pop stars. The private jet they travelled in was rented by Hangar 8, the AIM listed private jet management company, which yesterday provided a bullish trading update.
Its shares jumped 35p to a record 176.5p and have more-than-doubled since June following news that profits in the first quarter were up 331 per cent to £461,000. This performance moved the board to forecast that full year 2013 results will now be materially above market expectations with profits of £1.56million.
Shop broker Seymour Pierce lifted its target price to £2, advising clients the shares are significantly undervalued.
Hangar 8 manages 19 aircraft based at Oxford airport. Its aircraft usually carry up to ten passengers and fly in Britain, Europe, the former USSR and Middle East.
Hiring a Challenger Jet for a journey from Oxford to the South of France and back for eight people would cost you around £20,000. Punters do not have to contend with crowded airports and are generally airborne within two hours.
Hangar 8 is run by founder, 62 per
cent-shareholder and pilot Dustin Dryden. It provides jets at any time
and rents them out mostly to big businesses.
Middle East oil Sheikhs are major
customers too, preferring private jets than easyJet, 5p better at
659.5p, to take them to out of the way places. Marauding bears dragged
the Footsie 64.24 points lower to 5,722.01.
Strikes against austerity in the
southern eurozone, continuing concern about the impending 'fiscal cliff'
in the US and doom and gloom Bank of England governor Sir Mervyn King's
downgrading of growth forecasts for the UK economy, prompted renewed
selling. Wall Street didn't help, falling 185.23 points to 12,570.95 by
the closing bell.
Nomura sent a note to clients
fingering 16 stocks as potential takeover targets. It reckons funding
conditions in terms of market access and prices have eased significantly
and this can only be good for companies/private equity firms wishing to
embark on aggresive growth investments.
Informa, the Swiss-based publisher of
shipping guide Lloyd's list, is included on the Japanese broker's list.
It edged up 0.3p to 385.85p, but is still well down on October's peak
of 453p, attained when rumours of a £3.3billion or 550p a share cash
offer from Germany's Axel Springer were rife.
It's no surprise to see perennial
takeover favourite Invensys, 2.6p easier at 216.1p, on the list. It
reports interims today and consensus expectations are for an increase in
revenue of 1 per cent to £1.2billion, a 2 per cent fall in operating profits to £100million, a 23 per cent decline in earnings per share to 7.5p but a 6 per cent increase in dividend per share to 1.75p.
Software group Fidessa jumped 43p to
1318p following director buying. Chief executive Chris Aspinwall
acquired 10,441 and John Hamer 2,500 shares at the common price of
Strong interim results announced by
property group Great Portland Estates were counterbalanced by news of an
opportunistic placing of up to 31.25million shares, or 9.9 per cent of
the equity, to raise £136million for acquisitions in its core West End
market with three properties currently in detailed discussions totalling
£110million. Shares closed 1.3p easier at 458p.
Profit-taking in the wake of
better-than-expected third-quarter figures left set-top box maker Pace
10.3p lower at 184.35p. The management expect second-half revenues to be
around £113million, some 16 per cent up on the same period in 2011.
Support services group SIG firmed
2.7p to 105.3p after saying trading is OK and on track to meet full year
expectations. News that chief executive Chris Davies is to step down
after 19 years to be replaced by Stuart Mitchell who ran Wilkinson
Hardware Stores left dealers underwhelmed.
Fastnet Oil & Gas touched 34p
before closing 0.75p easier at 30.38p on profit-taking. Early optimism
followed the announcement of its award of a licensing operation in the
North Celtic Sea covering part of blocks 49/7-13, offshore Ireland.
Broker n1Singer said it strengthens Fastnet's portfolio further offshore
Ireland, one of the hottest areas of the industry right now.
Caledonia Mining added 0.5p at 7.5p on record third-quarter production and profits. Gold produced at the Blanket Mine in Zimbabwe reached a new all-time high of 12,918 ounces, 12 per cent higher than the 11,560 ounces produced in the quarter ended June 30.
- Better-than-expected first-half figures helped video content search engine Blinkx rise 2.25p to 73p. Adjusted pre-tax profits, which excluded acquisition costs and other one-off items, jumped to £5.3million from £3.1million. Broker Numis reiterated its 104p target saying operationally, the group has integrated the PVMG and Burst acquisitions ahead of schedule and introduced a new site for mobile and social media.
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