text:
Former Seattle Seahawks quarterback Tarvaris Jackson has been arrested in central Florida after authorities say he pulled a gun on his wife. An Osceola County Sheriff's Office arrest report says the 33-year-old Jackson had been visiting family in Kissimmee when the incident occurred early Friday. Jackson's wife told the deputy that Jackson had returned to their rental home drunk and began yelling at her, eventually pulling the gun and threatening to kill her.
An attorney handling Johnny Manziel's domestic violence case expressed doubts about the Heisman Trophy-winning quarterback's ability to stay clean and said he was given a receipt that shows Manziel may have spent more than $1,000 at a drug paraphernalia store just 15 hours after he was involved in a hit-and-run crash, according to a lengthy text message accidentally sent to The Associated Press. Defense attorney Bob Hinton's text indicated Manziel's legal team was seeking a plea deal with prosecutors, but suggested that could be tricky. Hinton also wrote that he had been emailed a ''heads up'' receipt ''which purports to reflect'' that Manziel made a purchase of $1,018.77 at a Gas Pipe store at 12:03 p.m. on Tuesday, less than a day after his crash.
Brandon Marshall is serving up some rather interesting, if maybe not particularly palatable, food combinations for his offseason diet. The New York Jets star wide receiver is trying to lose a few pounds, at coach Todd Bowles' request, before the team begins training camp late next month. The 6-foot-4 Marshall, who turned 32 in March, said Bowles wants him to play at under 230 pounds - his weight last season - this year after averaging around 234-235 the previous few years.
Clay Matthews, Julius Peppers and James Harrison will be interviewed next month by NFL officials in connection to a media report that linked them to the use of performance-enhancing drugs. A letter from NFL executive Adolpho Birch that was obtained Friday by The Associated Press says Green Bay linebackers Matthews and Peppers and Pittsburgh linebacker Harrison will be interviewed when training camps open. The Packers begin practicing on July 26, the Steelers on July 29.
PITTSBURGH (AP) -- Shaun Suisham's lengthy run with the Pittsburgh Steelers is over. It appears the longtime kicker's career may be in jeopardy, too.
LOS ANGELES (AP) -- Ben Affleck stands by his expletive-filled rant against the NFL and commissioner Roger Goodell over ''Deflategate.''
NASHVILLE, Tenn. (AP) -- Tennessee Titans linebacker Derrick Morgan wants the NFL to study how medical marijuana could help players.
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Moult cycle specific differential gene expression profiling of the crab
Crustacean moulting is a complex process involving many regulatory pathways. A holistic approach to examine differential gene expression profiles of transcripts relevant to the moulting process, across all moult cycle stages, was used in this study. Custom cDNA microarrays were constructed for
A total of 556 clones were sequenced from the cDNA libraries used to construct the arrays. These cDNAs represented 175 singletons and 62 contigs, resulting in 237 unique putative genes. The gene sequences were classified into the following biological functions: cuticular proteins associated with arthropod exoskeletons, farnesoic acid
The expression data presented here provide a chronological depiction of the molecular events associated with the biological changes that occur during the crustacean moult cycle. Tracing the temporal expression patterns of a large variety of transcripts involved in the moult cycle of
Moulting is a cyclic process that occurs in all arthropods, from insects to crustaceans, and is essential for growth, reproduction and metamorphosis. The crustacean moult cycle encompasses the period between two successive moults and has been subdivided into 4 major stages; intermoult, pre-moult, ecdysis, and post-moult
Moulting is regulated by an elaborate interplay of hormones, including those which promote, and those which negatively regulate moulting. Among the hormones involved in the induction of moulting are two families of nonpeptidergic hormones: the steroids (ecdysteroids), and the sesquiterpenoids (represented mainly by insect juvenile hormone (JH) and crustacean methyl farnesoate (MF))
The negative regulatory centre in crustaceans is the sinus gland/X-organ complex, a neurohaemal organ located in the eyestalk
Moulting is a complex process that is affected by a range of external and internal factors including temperature, photoperiod, nutritional state and eyestalk integrity. In order to explore the molecular events associated with the moulting process, microarray technology has been implemented to investigate differential gene expression in
Overview of
A total of 556 clones were sequenced from the cDNA libraries used to construct the
Figure 1
Distribution of sequenced cDNA transcripts isolated from the entire microarray study, grouped according to biological function
Distribution of sequenced cDNA transcripts isolated from the entire microarray study, grouped according to biological function.
The largest group of transcripts depicted here (31%) represents cDNAs that could not be annotated via the GenBank database. Transcripts encoding mitochondrial proteins such ATP synthase, cytochrome oxidases and NADH dehydrogenase make up 24% of the total cDNAs isolated in this study. Cuticular protein transcripts constitute 14%, while transcripts of the hemocyanin gene family and those related to muscle function and development comprise 6% each, of the total cDNA population. Phenoloxidase (PO) activators such as serine proteases, antimicrobial and clotting protein transcripts contribute to 5% of all sequenced cDNAs. Other transcripts encoding diverse proteins not classified into the other groups include ovary development-related protein, opsin, ferritin, heat shock protein, tubulin, notch protein, arginine and pyruvate dehydrogenase kinase, and transcripts that contained CT, GT or AC repeats, represent 5% of the total population. Lectins, such as the C-type lectin receptor and mannose-binding protein, as well as ribosomal proteins, each contributed to 3% of all sequenced cDNAs. Fatty acid binding protein and diazepam binding inhibitor transcripts, that are associated with lipid metabolism, constitute 2% of the overall transcript population, while FaMeT transcripts represent the smallest group that form 1% of all cDNAs sequenced within the scope of this microarray study.
Gene expression profiles across the moult cycle of
Moult stage-specific differential gene expression analysis was carried out on
Figure 2
Experimental design for moult stage hybridisations
Experimental design for moult stage hybridisations. Each rectangle represents an array with 3 individuals pooled from each stage; each array was repeated 3 times with different individuals. The green colour represents the label Cy3 while orange represents Cy5 (M = moult, P = post-moult, I = intermoult, E = early pre-moult, L = late pre-moult).
Analysis with GeneSpring using K-means clustering revealed seven main subsets of transcripts that displayed differential expression profiles across the moult cycle of
The transcripts assigned to Cluster A are relatively down-regulated at the time of moulting, expression subsequently increases at each consecutive stage, peaking in early pre-moult then plateauing or declining slightly in late pre-moult in preparation for ecdysis. In this subset, four clusters (A1-A4) were deemed to have similar expression profiles and hence collectively termed Cluster A; this group is shown in Figure
Figure 3
K-means clustering maps of the collective clusters featuring time series expression profiles which vary according to moult stage
K-means clustering maps of the collective clusters featuring time series expression profiles which vary according to moult stage. Fold change in expression is depicted on the vertical axis and moult stages are depicted on the horizontal axis whereby M = moult, P = post-moult, I = intermoult, E = early pre-moult, L = late pre-moult.
Table 1
Summary of the genes contained within Cluster A.
Transcript ID - Annotation
Accession #
Transcript #
Cluster A1
cytochrome b & cytochrome c oxidase
Cluster A2
Cluster A3
ATP synthase, cytochrome b & cytochrome c oxidase, NADH dehydrogenase
40 S ribosomal protein S12 [
CT repeat
Cluster A4
ATP synthase, cytochrome b & cytochrome c oxidase
putative elongation factor 1 beta' [
Transcript # indicates the number of copies of each gene present within the cluster.
Cluster B consists of transcripts which are down-regulated in the moult and post-moult stages, expression then increases dramatically in intermoult, remains high in early pre-moult and begins to decline in late pre-moult. This group is depicted in Figure
Table 2
Summary of the genes contained within Cluster B.
Transcript ID - Annotation
Accession #
Transcript #
Cluster B1
Cluster B2
Cluster B3
Transcript # indicates the number of copies of each gene present within the cluster.
Cluster C is a relatively small group of transcripts (ten in total), in which expression is down-regulated in the moult and post-moult stages, increases substantially in intermoult, then drops dramatically in early and late pre-moult (Figure
Table 3
Summary of the genes contained within Cluster C.
Transcript ID - Annotation
Accession #
Transcript #
Cluster C
Transcript # indicates the number of copies of each gene present within the cluster.
The transcript profile that is represented in Cluster D displays expression that peaks in the post-moult stage, decreases dramatically in the intermoult and early pre-moult stages, then begins to increase again in late pre-moult and ecdysis. Figure
Table 4
Summary of the genes contained within Cluster D.
Transcript ID - Annotation
Accession #
Transcript #
Cluster D1
Cluster D2
Cluster D3
Cluster D4
Transcript # indicates the number of copies of each gene present within the cluster.
The group of transcripts represented in Cluster E display expression profiles which are relatively low during the moult, post-moult and intermoult stages, then increase in the early pre-moult stage and remain high in late pre-moult. This group is depicted in Figure
Table 5
Summary of the genes contained within Cluster E.
Transcript ID - Annotation
Accession #
Transcript #
Cluster E1
Cluster E2
Transcript # indicates the number of copies of each gene present within the cluster.
Cluster F is depicted in Figure
Table 6
Summary of the genes contained within Cluster F.
Transcript ID - Annotation
Accession #
Transcript #
Cluster F
Transcript # indicates the number of copies of each gene present within the cluster.
The expression profiles of the transcripts comprising Cluster G show relatively low expression levels in the moult stage, an increase to peak levels in the post-moult and intermoult stages, then a dramatic decrease in the early pre-moult stage which begins to increase again in late pre-moult. The gene expression pattern for Cluster G is presented in Figure
Table 7
Summary of the genes contained within Cluster G.
Transcript ID - Annotation
Accession #
Transcript #
Cluster G
Transcript # indicates the number of copies of each gene present within the cluster.
Figure 4
Overview of the temporal gene expression profiles observed for each cluster across the moult cycle
Overview of the temporal gene expression profiles observed for each cluster across the moult cycle.
A holistic approach to gene expression profiling was employed in order to gain a greater understanding of the molecular events associated with the crustacean moulting process. A
Genisphere's 3DNA dendrimer labeling technology, which overcomes some difficulties associated with direct or indirect labelling methods, was used in this study. This technology allows the labelling of cDNA via a target capture sequence oligonucleotide rather than incorporating the fluorescent dye directly during cDNA preparation, thus avoiding inefficient synthesis and hybridization of the cDNA to the array that results from the incorporation of fluorescent dye nucleotide conjugates into the reverse transcript. Additionally, the signal generated from each message will be independent of base composition or length of the transcript (Genisphere 3DNA Array 900MPX protocol).
The sequencing of 556 clones from two libraries (whole crab and crab organ) generated from
Cellular energy requirements across the moult cycle
Transcripts representing mitochondrial proteins, such as ATP synthase, cytochrome oxidase, and NADH dehydrogenase form the second largest group (24%) of cDNAs isolated during this microarray study (Figure
A similar expression profile occurs for further transcripts of ATP synthase, arginine kinase and fumerase (found in Cluster B, Figure
Cuticular protein expression
Transcripts encoding cuticular proteins represent 14% of the total cDNAs isolated in this microarray study. Several patterns of moult cycle differential expression were observed for cuticular proteins, implying that each group has a specific but varying function depending on which stage of the moult cycle up-regulation is detected. For instance a peak in expression during intermoult was found to occur for cuticle proteins CUT7 and CUT8 (EF101999, EF102000) (Cluster C, Figure
A similar pattern is seen in Cluster F which contains 13% cuticle protein transcripts, composed of
Additionally, two transcripts that displayed similarity to a low-density lipoprotein receptor class A (LDLa) domain containing chitin binding protein from
Differential expression of the hemocyanin gene family
Transcripts belonging to the hemocyanin gene family represented 6% of all sequenced cDNAs (Figure
Cryptocyanin is structurally related to hemocyanin however it lacks the ability to bind oxygen
Phenoloxidase activity
PO activators such as the serine proteases trypsin, chymotrypsin, and trypsinogen, in addition to antimicrobial and clotting proteins, made up 5% of the total distribution of sequenced cDNAs (Figure
Trypsin and chymotrypsin expression correlates strongly with hemocyanin expression (Cluster B), and may be involved in activation of the PO pathway and the stimulation of hemocyanin into an active phenoloxidase-like enzyme, that is associated with melanin synthesis and sclerotization in the newly developing cuticle in
Genes involved in cuticle hardening
Lectins, which include the calcium dependant lectin group (C-type lectin) receptor, mannose-binding protein, mucin and a proline rich protein, represent 3% of the cDNAs isolated in this study (Figure
Glycoproteins, such as the mannose rich variety found in the calcified cuticle of
Lectins (carbohydrate binding proteins) are also involved in immune function through the lectin-complement pathway, in which the mannose-binding lectin recognises infectious agents and triggers PO activation
Muscle formation
Muscle-related cDNAs such as actin, myosin and thymosin, constituted 6% of all the transcripts isolated during the moult cycle-related microarray experiments (Figure
Lipid metabolism
Transcripts encoding the lipid metabolism proteins diazepam-binding inhibitor and fatty acid binding protein constituted 2% of all sequenced transcripts (Figure
Tracing the temporal expression patterns of genes involved in the crustacean moult cycle provides a platform for gaining a greater understanding of gene function, interaction, and regulation with respect to the moulting process. The expression data presented here provide a chronological depiction of the molecular events associated with the biological changes occurring during the crustacean moult cycle.
Transcripts associated with energy production, such as mitochondrial and ribosomal genes, increased in expression as the moult cycle progressed. ATP synthase catalyses the synthesis of ATP (the fundamental means of cell energy production) via a proton gradient
A number of genes likely to play an important role in the formation and hardening of the crustacean exoskeleton, such as cuticle proteins, PO activators, lectins, fatty acid-binding proteins and members of the hemocyanin family, have been identified by virtue of protein domain annotation and differential gene expression data. These genes display expression profiles specific to function across the moult cycle. Temporal variation in expression has even been observed between individual cuticular protein transcripts containing the same protein domains (such as chitin or calcium binding). This suggests a difference in functionality for each gene, indicating that transcripts from a similar group may play a distinct and different role in the formation of the crustacean exoskeleton.
Glycosylation of cuticular proteins in the crustacean exoskeleton has been implicated in the regulation of cuticle calcification
A large diversity of genes representing many important biological functions related to moulting in crustaceans were able to annotated, and their expression profiles mapped across consecutive stages of the moult cycle of
Animal selection
cDNA library construction
Two cDNA libraries were constructed using various source tissues, selected in order to provide a diverse collection of transcripts, and representing a broad range of tissue functions and physiological states in all moult stages. One of the cDNA libraries was synthesised from whole animals in order to obtain transcripts from each tissue type. For this library, six small crabs, from each of the following five moult stages; moult, post-moult, intermoult, early and late pre-moult stages, were selected, snap frozen and individually ground under liquid nitrogen. The second cDNA library was derived from organs previously identified as being important to the moult cycle of crustaceans and served to enrich the array with sequences particularly relevant to crustacean moulting. The tissues represented in the
Total RNA was purified from each sample using TRI
A commercial cDNA library synthesis system (SMART cDNA library construction kit, Clonetech, Mountain View, CA, USA) was used for the construction of each library according to the manufacturer's instructions. Only the final cloning step was modified so that instead of using the λ TriplEx2 vector supplied with the kit, the size fractionated cDNA was ligated into pGEM-T Easy (Promega, Madison, WI, USA) according to the manufacturer's instructions, and transformed into XL10 Gold ultracompetent cells (Stratagene, La Jolla, CA, USA) according to the manufacturer's protocol. 80 clones, randomly selected from each library, were then sequenced and analysed using BLAST
Screening for redundant clones
Upon examination of the sequences of 160 clones, from the cDNA libraries of both whole crab and crab organ, redundancies for 16 S ribosomal RNA (rRNA) transcripts were found to be as high as 30%. To remove 16 S rRNA carrying plasmids, all of the clones were first screened for the 16 S rRNA sequence, using a colony hybridisation method
Construction of custom
5000 unsequenced clones, that had been pre-screened for 16 S rRNA, were randomly selected for spotting onto the microarray slides. 2400 were selected from the whole crab library and 2600 from the crab organ library. These were grown overnight in LB containing 50 μg/ml ampicillin. The clones were sent to the AgGenomics (Bundoora, Vic, Australia) microarray printing facility. The clones were PCR amplified using kit-supplied primers (Clontech) and contact-spotted (in duplicate) using pins, onto amino silane coated glass slides, in a 50% DMSO buffer. Known crab genes, that were identified at the initial sequencing stage, such as actin (GenBank accession
Experimental Design
In order to identify differential gene expression across moult stages, two consecutive moult stages were compared on each array in a dual colour (Cy3 and Cy5) experiment. RNA samples (isolated from individual crabs) were pooled across subjects in order to reduce the effect of biological variation. A formula, that dictates the total number of subjects and arrays required for the pooled experiment to obtain gene expression estimates and confidence intervals comparable to those obtained from a non-pooled experiment
Spatial variation within each array was addressed through spot duplication. Two identical blocks of grids consisting of each amplified cDNA and including the controls described above were printed onto the left and right sides of each horizontally orientated array, thus affording spatial separation between duplicate spots, to allow for the normalisation of potential hybridisation anomalies.
Microarray Hybridisations
Nine small crabs (six of these were also used in the above described whole crab cDNA library construction) were snap frozen, individually ground under liquid nitrogen and RNA was isolated from each ground crab using TRI
Data analysis
Microarray slides were scanned using a white-light ArrayWorx Biochip Reader (Applied Precision, LLC, Issaquah, Washington, USA). ImaGene (BioDiscovery Inc., El Segundo, CA, USA) was then used to process images and create spot intensity reports, while CloneTracker (Biodiscovery Inc.) was used to generate gene ID mapping files and assign gene identification. Final intensity reports were retrieved as raw spot intensities in tab-delimited files. The data set is deposited in the Gene Expression Omnibus (GEO) database (accession no. GSE6997) at the following site:
Microarray data analysis was performed using GeneSpring GX 11.0 (Agilent Technologies Inc., Santa Clara, CA, USA). The single colour workflow feature of GeneSpring GX was used in order to split the two channel array into 2 single colour experiments to enable the analysis of multiple samples across different arrays. Using the loop design depicted in Figure
The two colour data was normalised using the robust scatter plot smoother LOESS (also known as "LOWESS" for locally-weighted regression and smoothing scatter plots)
Sequence and phylogenetic analysis
Following hybridisation experiments, clones that displayed differential expression (P ≤ 0.05) patterns across moult stages were sequenced. Overlapping sequences (contigs), that likely represent the same cDNA, and clones without sequence identity to other cDNAs (singletons) were identified by comparing all sequences against one another in Sequencher (Gene Codes Corporation, Ann Arbor, MI, USA). The genes were annotated with the name of the highest basic local alignment search tool (BLAST)
Authors' contributions
AK performed the moult staging, animal husbandry and sampling, library preparation, sequencing, bioinformatic analysis of the ESTs, microarray construction, microarray hybridizations, and interpretation of the data, contributed to the experimental design and drafted the manuscript. TH contributed to the construction of the microarrays, the microarray hybridizations and analysis. BP contributed to the conception and design of the study and provided the experimental animals. AE contributed to the conception and design of the study, coordinated the work, data interpretation and drafting of the manuscript. All authors read and approved the final manuscript.
We are grateful to the Bribie Island Research Centre crab group for providing the experimental animals used in this study, Tim Sawbridge for advice concerning microarray analysis and Hazra Thaggard for laboratory management.
The project was funded by the Queensland Government Aquaculture Industry Development Initiative grant to AE. This funding source covered the costs of a PhD stipend for AK and all the project costs. AE (0.5), BP and TH were funded by the Queensland government Department of Employment, Economic Development and Innovation, and the University of the Sunshine Coast (AE, 0.5).
Mue et cycle d'intermue chez les crustacés décapodes
Moulting and Regeneration
A remarkable, precisely timed release of hyperglycemic hormone from endocrine cells in the gut is associated with ecdysis in the crab Carcinus maenas
Early pattern of calcification in the dorsal carapace of the blue crab, Callinectes sapidus
The structure and calcification of the crustacean cuticle
A new mechanism for the control of phenoloxidase activity: inhibition and complex formation with quinone isomerase
Comparative endocrinology of molting and reproduction: insects and crustaceans
Insect Hormones
Methyl farnesoate controls adult male morphogenesis in the crayfish,
Role of ecdysteroids and methyl farnesoate in morphogenesis and terminal moult in polymorphic males of the spider crab
Effect of methyl farnesoate on late larval development and metamorphosis in the prawn
Vitellogenesis in the Red Crab,
Vitellogenesis in the red crab
Effect of methyl farnesoate, alone and in combination with other hormones, on ovarian growth of the red swamp crayfish,
Stimulation of ovarian maturation in the crayfish
Methyl farnesoate stimulates ecdysteroid secretion from crab Y-Organs
Regulation of crustacean moulting
Mandibular Gland of the Blue Crab
Crustacean Endocrinology, A Review
Effects of bilateral eyestalk ablation on molting and ovarian development in the giant freshwater prawn,
Ecdysteroid Biosynthesis in the Crustacean Y-Organ and Control by an Eyestalk Neuropeptide
Crustacean neuropeptide genes of the CHH/MIH/GIH family: implications from molecular studies
Biochemical and functional aspects of crustacean hyperglycemic hormone in decapod crustaceans: Review and update
Molecular, cytological and physiological aspects of the crustacean hyperglycemic hormone family
Crustacean hyperglycemic hormone (CHH) neuropeptides family: Functions, titer, and binding to target tissues
Purification, characterisation and amino acid composition of the putative moult-inhibiting hormone (MIH) of Carcinus maenas (Crustacea, Decapoda)
Neuropeptides Inhibiting Growth and Reproduction in Crustaceans
High-affinity binding of putative moult-inhibiting hormone (MIH) and crustacean hyperglycaemic hormone (CHH) to membrane-bound receptors on the Y-organ of the shore crab
A Neurohormone Regulating both Methyl Farnesoate Synthesis and Glucose Metabolism in a Crustacean
Structure and significance of mandibular organ-inhibiting hormone in the crab,
Function and cellular localization of farnesoic acid O-methyltransferase (FAMeT) in the shrimp,
Hemolymph ecdysone and electrolytes during the molting cycle of crayfish: A comparison of natural molts with those induced by eyestalk removal of multiple limb autotomy
The mamalian mitochondrial respriatory chain
Search for hepatopancreatic ecdysteroid-responsive genes during the crayfish molt cycle: from a single gene to multigenicity
Direct regulation of mitochondrial RNA synthesis by thyroid hormone
Ecdysteroid Titers During the Molt Cycle of the Blue Crab Resemble those of other Crustacea
Evolution and physiological roles of phosphagen systems
On the role of arginine kinase in insect flight muscle
Purification and characterization of arginine kinase from the mantle muscle of the squid, Symplectoteuthis oualaniensis : Role of the phosphagen/phosphagen kinase system in a highly aerobic muscle
Expression of arginine kinase enzymatic activity and mRNA in gills of the euryhaline crabs Carcinus maenas and Callinectes sapidus
Arginine Kinase Expression and Localization in Growth Cone Migration
The distribution of guanidine-adenosine triphosphate phosphotransferases and adenosine triphosphatase in animals from several phyla
Gene expression profiling of cuticular proteins across the moult cycle of the crab Portunus pelagicus
Exoskeletal proteins from the crab,
Classes of integument peptides
A gastrolith protein serving a dual role in the formation of an amorphous mineral containing extracellular matrix
A crustacean Ca2+-binding protein with a glutamate-rich sequence promotes CaCO3 crystallization
Cloning and expression of a cDNA encoding a matrix peptide associated with calcification in the exoskeleton of the crayfish
Identification of two new peritrophic membrane proteins from larval Trichoplusia ni: structural characteristics and their functions in the protease rich insect gut
Recent advances in crustacean genomics
Evolution of arthropod hemocyanins and insect storage proteins (hexamerins)
Conversion of crustacean hemocyanin to catecholoxidase
Hemocyanin in the exoskeleton of crustaceans: enzymatic properties and immunolocalization
Binding proteins for an ecdysone metabolite in the crustacean hepatopancreas
Spider hemocyanin binds ecdysone and 20-OH-ecdysone
Evolution of novel functions: cryptocyanin helps build new exoskeleton in
Hemolymph proteins and molting in crustaceans and insects
Role of a copper-specific metallothionein of the blue crab,
MTPA: A crustacean metallothionein that affects hepatopancreatic mitochondrial functions
Effects of molting and environmental factors on trace metal body-burdens and hemocyanin concentrations in the American lobster, Homarus americanus
Cloning, tissue expression and metal inducibility of an ubiquitous metallothionein(1) from Panulirus argus
Anatomy and physiology of digestive tract of Crustaceans Decapods reared in aquaculture
Digestion physiology and proteolytic enzymes of crustacean species of the Mexican Pacific Ocean
Molecular cloning and sequencing of trypsin cDNAs from Penaeus vannamei (Crustacea, Decapoda): Use in assessing gene expression during the moult cycle
Differential expression of trypsin mRNA in the white shrimp (Penaeus vannamei) midgut gland under starvation conditions
Functional and phylogenetic analyses of phenoloxidases from brachyuran (
Regulation of insect hemolymph phenoloxidases
Unified mechanism for sclerotization of insect cuticle
Phenoloxidase specific activity in the red swamp crayfish Procambarus clarkii
Tyrosinases from crustaceans form hexamers
Hemocyanin-derived phenoloxidase activity in the spiny lobster Panulirus argus (Latreille, 1804)
Postecdysial cuticle alteration in the blue crab,
Purification of a soluble glycoprotein from the uncalcified ecdysial cuticle of the blue crab Callinectes sapidus and its possible role in initial mineralization
Differential expression profiling of components associated with exoskeletal hardening in crustaceans
How C-type lectins detect pathogens
The lectin-complement pathway--its role in innate immunity and evolution
Melanogenesis and the generation of cytotoxic molecules during insect cellular immune reactions
Actin molecular structure and function
Myosins: a diverse superfamily
Regulation of muscle growth and sarcomeric protein gene expression over the intermolt cycle
Integument and Exoskeleton
The lipid composition of hypodermal membranes from the blue crab (
Characterization of a hemocyte intracellular fatty acid-binding protein from crayfish (
The molecular mechanism of ATP synthesis by F1F0-ATP synthase
The respiratory-chain NADH dehydrogenase (complex I) of mitochondria
Cuticular proteins from the blue crab alter in vitro calcium carbonate mineralization
The crustacean molt cycle and hormonal regulation: It's importance in soft shell blue crab production
Isolation and expression analysis of multiple isoforms of putative farnesoic acid O-methyltransferase in several crustacean species
The efficiency of pooling mRNA in microarray experiments
Normalization of cDNA microarray data
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
The Pfam protein families database

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March 24, 2014 - 12:01pm
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Mass held for Merle & Lawrence Klug, former business owners, community servants
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March 18, 2014 - 4:39pm
Sen. Vinehout column: Lobbyists and Corporations 2; Voters 0
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March 18, 2014 - 10:49am
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Bill Parks for Pierce County Sheriff fundraiser
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March 13, 2014 - 10:11am
February 18, 2014
'Call to Caucus' for GOP March 1
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February 18, 2014 - 10:03am
February 17, 2014
River Falls mayoral candidate Mike Wharton: The rest of his response
The Feb. 13 print edition of the River Falls Journal accidentally left off some final comments by Mike Wharton who is running in the Feb. 18 mayor's primary in River Falls. The following are the omitted portions of Wharton's response about his candidacy: There is an opportunity...
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February 17, 2014 - 8:42am
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Administrator, mayor 'state' what's up for city
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January 23, 2014 - 10:32am
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Only one race set for Pierce County Board
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January 8, 2014 - 11:05am
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Playstation 2 Games
Unlock Theater Option for Shadow Hearts: CovenantHome / Playstation 2 /
This page contains Shadow Hearts: Covenant Unlockables for Playstation 2 called "Unlock Theater Option" and has been posted or updated on Jan 2, 2008 by overlord15.
Boxshot & Details
- Developer: Nautilus
- Publisher: Midway
- Genre: Role-Playing Console-style RPG
- Release: Sep 27, 2004
- ESRB: Teen
Unlock Theater Option
Get true the game once.
Report Problem
Please Submit a Problem for any incomplete, non-working or fake cheat listed above.
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From: sboy120
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From: mooda
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Trailers & Gameplay Videos
Shadow Hearts: Covenant - Dark Seraphim Battle
- May 31, 2014, Views: 95
Shadow Hearts: Covenant - Grand Papillon the Bandit
- May 31, 2014, Views: 120
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- May 31, 2014, Views: 125
View All Videos
Shadow Hearts: Covenant - The Weak Points of a Hero
- May 31, 2014, Views: 46
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OasiMaremma Village, Grosseto (province)
- Free WiFi in lobby and free parking
Main amenities
- 48 guestrooms
- Restaurant and bar/lounge
- Outdoor pool
- Breakfast available
- Free supervised kid's activities
- Free shuttle
- Air conditioning
- Garden
- Computer station
- Snack bar/deli
- Self-serve laundry
- Multilingual staff
What's around
- Castello di Scarlino (1.9 mi / 3 km)
- Scarlino Marina (3.7 mi / 5.9 km)
- Naturalistic Mining Park of Gavorrano (4.8 mi / 7.7 km)
- Church of San Leopoldo (5.7 mi / 9.2 km)
- MAGMA Museum of Cast Iron Arts of Maremma (5.7 mi / 9.2 km)
For families
- Cribs/infant beds (surcharge)
- Supervised childcare/activities
- Playground on site
- Supervised childcare/activities (free)
- Kitchen
- Private bathroom
Good establishment and well located. Go there.
Perhaps this would be a better ratingif it was presented differently, but expectations…
OasiMaremma Village, Grosseto (province)
Hotel Info: 866-539-8117from $97
- Standard Apartment, View
- Apartment, View
- Deluxe Apartment, 1 Bedroom
- Standard Studio, View
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- Deluxe Apartment, 2 Bedrooms
Also popular with travelers from the USA
At a glance
Key facts
Hotel size
- This hotel has 48 rooms
- Check-in time 4 PM-7:30 PM
- Check-out time is 10 AM
- Express check-in/out
Required at check in
- Credit card deposit required
- Government-issued photo ID required
- Minimum check-in age is 18
Travelling with others
- Children (2 years old and younger) stay free when occupying the parent or guardian's room, using existing bedding
- Pets not allowed
- Free WiFi in public areas
- Free self parking
- Free beach shuttle
Nearby One Star Grosseto (province) hotels
In the hotel
- Supervised childcare/activities (free)
- Buffet breakfast daily (surcharge)
- Restaurant
- Bar/lounge
- Snack bar/deli
- Poolside bar
- Free beach shuttle
- Outdoor pool
- Playground on site
- Sailing nearby
- Scuba diving nearby
- Snorkeling nearby
- Windsurfing nearby
- Computer station
- Front desk (limited hours)
- Tours/ticket assistance
- Laundry facilities
- Luggage storage
- Wedding services
- Multilingual staff
- Number of buildings/towers - 22
- Year Built 2002
- Garden
In the room
- Air conditioning
- Minibar
- Balcony or patio
- Private bathroom
- Shower only
- Satellite TV channels
- Kitchen
- In-room safe
Top Grosseto (province) hotels
Recently reviewed hotels in Grosseto (province)
- Pian dei Mucini Resort Hotel VittoriaFrom
"overall a good resort. dinner and breakfast buffet are good. two big pools are nice too. downsides: room was a little smelly and the WiFi in the resort area is very weak."Jun 4, 2016
- Fattoria San Lorenzo - ApartmentsFrom
"No hot water"Nov 7, 2015
- Hotel I CrespiFrom
"The hotel is very modern, clean, and confortable. Be aware that the front desk closes in the evenings and thus it is very important to call in advance if arriving past 10pm."Nov 1, 2015
- Grand Hotel BastianiFrom
Nearby hotels
Special features
DiningOasiMaremma - This poolside restaurant serves breakfast and dinner. Guests can order drinks at the bar and enjoy alfresco dining (weather permitting). A children's menu is available.
OasiMaremma Village, Grosseto (province)'s small print
Also known as
- OasiMaremma Village
- OasiMaremma Village Inn
- OasiMaremma Village Inn Scarlino
- OasiMaremma Village Scarlino
Cash transactions at this property cannot exceed EUR 2999.99, due to national regulations. For further details, please contact the property using information in the booking confirmation.
Only registered guests are allowed in the guestrooms.
This property does not have elevators.
The name on the credit card used at check-in to pay for incidentals must be the primary name on the guestroom reservation.
Mandatory fees
You'll be asked to pay the following charges at check in or check out:
- Towels/sheets: EUR 7 per accommodation, per stay
Optional extras
Buffet breakfast is offered for an extra charge of EUR 8 per person (approximately)
Cribs/Infant beds are available for EUR 40.00 per week
We have included all charges provided to us by this hotel. However, charges can vary, for example, based on length of stay or the room you book.
Is the description of this hotel not correct? Tell us


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text: Pull a Record (Prod. by Still Soul) by App- published on 2011-08-24T18:19:38Z Genre hip hop Comment by Still Soul @the-apparition: Yea man I got kick and snare samples from real drums 2011/09/16 01:16:47 +0000 Comment by App- @sondericker: they were live drums originally, Still Soul just threw em in his beat machine to create the beat 2011/09/11 23:42:12 +0000 Comment by App- @sondericker: word up, glad u dig it 2011/09/11 23:38:15 +0000 Comment by Soundwrecker Live drums or synth? Sound live to me. ;) 2011/09/11 17:25:22 +0000 Comment by Soundwrecker Oh yeah, weird and beautiful. 2011/09/11 17:24:22 +0000


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D7100 and 16-85
Started Apr 13, 2013 | Discussions thread
Shop cameras & lenses ▾
ForeignerOnEarth • Regular Member • Posts: 223
Re: D7100 and 16-85
In reply to demarren 123 • Apr 13, 2013
16-85 is much better than 18-200 on D7100. I have 16-85 many years and I have bought D7100 now. I tried 18-200 on D300 and on 12 MPx is difference between 16-85 and 18-200, we can expect much bigger difference on 24MPx D7100.
ForeignerOnEarth's gear list:ForeignerOnEarth's gear list
Nikon D300 Nikon 1 J1 Nikon D7100 Nikon AF-S Nikkor 70-200mm f/2.8G ED VR Nikon AF-S DX Nikkor 16-85mm f/3.5-5.6G ED VR +16 more
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text:
Vuissa said the festival has always tried to "have a very broad approach" to the films that LDS filmmakers are producing. Although the festival has a policy not to show R-rated content, some subject matter addressed in the past, including topics such as drug abuse in Utah County, may not be suitable for broad audiences, he said.
There will be question-and-answer sessions following each film, and forums and presentations during the day on Friday and Saturday.
Vuissa is impressed with the advancement he sees in the industry. He doesn't have his own entry this year, but he isn't worried about that.
"I'm a peer, just as much a beginner as anybody else," he said. "I enjoy watching some of my students' work."
Vuissa said he's watched several successful filmmakers start out at the festival and go on to enjoy great success with films such as "Saints and Soldiers" and "The Saratov Approach."
"The future becomes more and more exciting," he said. "The industry is evolving."
If you go:
What: 2014 LDS Film Festival
Where: SCERA Center for the Arts, 745 S. State St., Orem
When: Feb. 5-8, with Wednesday night opening at 7 p.m., and feature films and competitions throughout Thursday-Saturday
Cost: $50 all-event pass, $20-$30 for individual day passes
Website: ldsfilmfestival.org
Sharon Haddock is a professional writer with more than 35 years' experience, 17 at the Deseret News. Her personal blog is at sharonhaddock.blogspot.com.
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Home health care costs hit $83.2 billion for patients in 2014, the vast majority of which was covered by Medicare and Medicaid plans. However, the Center for Medicare Services claims that approximately 60 percent of home health claims in 2015 were possibly filed improperly, which can sometimes be an indicator for fraud. The CMS plans to address this by reviewing every single claim for home health care rather than the 20 percent that has been standard in the past.]]> At face value, carefully reviewing each claim for possible indications of fraud might seem like a good idea, but it can have a profoundly negative impact on patients. A delay in processing due to reviews can leave patients unsure of their current statuses and even possibly delay some claims for extended time periods. Furthermore, increased scrutiny also raises the opportunity for home health providers to be wrongly accused of submitting false claims.
Almost 1,800 defendants have been convicted of committing felony health care fraud in the past 11 years. A conviction for this type of charge can result in years spent behind the bars of a federal prison, as well as hefty fines that can prove to be difficult to repay. Although handling a federal charge rather than one under the jurisdiction of a Texas state court can be complicated, taking the necessary time to review charges and formulate a strategic defense plan can be especially useful.
Source: The Dallas Morning News, "Texas home health care providers will soon face extra scrutiny to prevent fraud", Sabriya Rice, June 17, 2016]]>
In general, the amount that bail is set at is based upon an alleged criminal charge and not upon a person's actual ability to pay. Even though the law presumes that all defendants are innocent unless proven otherwise beyond a reasonable doubt, 6,800 individuals are currently incarcerated in a single Texas county simply for not being wealthy enough to pay. Advocates for these and other individuals claim that this system unfairly marginalizes the poorest citizens while giving otherwise wealthy alleged offenders a pass to pay up and walk free.]]> The impact of being held on bail can be seriously damaging. Many people who are unable to pay cite further financial strain on their employment, family and housing situation. In the case of a mother who was charged with failing to have a proper ID behind the wheel, her inability to pay a $5,000 bail separated her from her two young children -- one of whom has special needs and therefore requires specialized care -- for nearly a week.
While it might be difficult to understand how a mother can be legally separated from her children for an otherwise minor charge, the 55 people who could not pay bail and later died in jail from 2009 to 2015 might be even more disconcerting. An advocate group is currently taking action in an attempt to change how bail is set. For the time being, most Texas defendants must still contend with the realities of having bail set for state crime charges. However, a person's ability to pay does not necessarily indicate that they will proceed in a certain manner, and careful preparation alongside their respective counsels can help ensure that they achieve the most favorable outcome possible.
Source: salon.com, "Criminal injustice in Texas: Thousands stay jailed in just one county because they can't pay bail and it's happening all over the U.S.", Eesha Pandit, June 5, 2016]]>
Texas police claim that the graffiti went beyond a simple prank. One of the officers involved in the investigation claims that some of the messages were racially insensitive and featured offensive gender overtones. Not all of the graffiti contained words, and at least one image was supposedly in the shape of male genitalia. Another area that was spray painted eluded to the idea that the graffiti might have been some form of payback for another incident.]]> The teenage boys, all of whom are aged 17 to 18 years old, were arrested and held behind bars on $2,500 bail. Prior to their arrests, Texas police believe that one of the teens drove all of them to a neighborhood near the high school, from where they then walked to the building. However, the boys also claim that much of the graffiti located on the school's buildings was already present before they arrived.
Because of the boys' ages, all of the teens will have to face their charges as adults rather than juveniles. A felony charge for allegedly defacing a school can have serious consequences, including as long as two years behind bars and a $10,000 fine. This can be exceptionally daunting for young individuals who have their entire futures ahead of them, making it wise for defendants to undertake careful preparation for trial alongside their respective counsel.
Source: breitbart.com, "Alleged 'Senior Prank' Lands Five Texas Teens in Jail on Felony Charges", Merrill Hope, May 28, 2016]]>
A recent article details the arrest of three Texas law officers and a U.S. Border Patrol agent in a four-year federal drug investigation. Police allege that the four officers have been linked to a large-scale cocaine operation. Each defendant is accused of conspiracy to possess with intent to distribute greater than 5 kilograms of cocaine.]]> Additionally, they face charges of possession of cocaine with intent to distribute. According to the Texas Tribune, the border patrol agent is specifically accused of the intent to distribute 17 kilograms of cocaine. Each defendant could face up to life in a federal penitentiary and hefty fines if convicted of the alleged crimes.
In such stressful situations, those accused of a crime want to ensure that their rights are protected, and that they are treated fairly by authorities and legal system throughout the process. Those accused of possession with intent to distribute may want to see legal representation from an attorney who is experienced in criminal law and defending cases involving drug charges. A Texas attorney who focuses on this kind of law may be the best place to start in order to understand applicable laws that could affect the outcome of a trial.
Source: The Dallas Morning News, "Four Texas law officers, including Border Patrol agent, indicted on drug charges", Chris Siron, May 24, 2016]]>
According to a former patient, the main doctor at the clinic claimed to be a survivor of stage IV cancer that she had ultimately cured herself. That same patient claims that she shelled out $650 per treatment that cost hundreds of dollars less in a different state. Her partner allegedly researched her background on the internet and came to his own conclusion that she had possibly stolen another person's identity in order to cash in on cancer treatments.]]> Local police utilized a recently executed search warrant to further its investigation alongside the DEA. Little information from the investigation has been made available to the public. The patient who spoke out about the cost of her alternative cancer treatment also expressed disappointment in the fact that the clinic has thus far remained open, and made further assertions that she had almost died while in their care.
Posing as a doctor is an understandably serious allegation that might anger cancer patients currently undergoing treatment, whether in Texas or elsewhere. While an emotional reaction to this type of white collar crime is understandable, mere allegations are never proof of guilt. Each and every defendant in Texas has the right to the presumption of his or her innocence throughout criminal process, including the investigation and any subsequent trial court proceedings.
Source: ksat.com, "Boerne clinic allegedly run by fake doctor still open for business", Alec Schreck, May 11, 2016]]>
Several agencies teamed up to complete the multi-year operation that resulted in the 13-count indictment. Police seized over nine tons of synthetic drugs and millions of dollars during that period of time. The synthetic drugs were supposedly packaged in small bags under various names, including Kush and potpourri, and sold at places like convenience stores and gas stations. Authorities claim that synthetic drugs are causing deaths across the state, but they did not link any of those alleged deaths to the recent arrests.]]> One of those arrested includes a professor at an area university. The professor of finance is also accused of conspiring to launder money in addition to the distribution charges. It is not entirely clear if the other individuals listed in the indictment are also facing different charges in addition to the initial allegations of distribution.
Whether synthetic or not, the legal system in Texas treats allegations for drug distribution quite seriously, and the possible penalties for a conviction can affect a person for the remainder of his or her life. Defendants can typically achieve the best outcome for their unique cases by taking the time to thoroughly review their charges and related evidence. Completing this in a timely manner allows most people to determine whether it is in their best interests to negotiate a plea deal or to fight the charges to their fullest extent.
Source: abc13.com, "UH professor among those arrested in synthetic drug bust", May 18, 2016]]>
The FBI launched an investigation in Dec. 2010 after learning of a possible cheating scheme going on at the school district. Prior to the FBI's involvement, the district was allegedly already aware of a possible scandal after it was discovered that the transcripts of nearly 80 students had been falsified. The district denied any type of wrongdoing at that time.]]> The purpose behind the alleged altered transcripts was to apparently boost the school district's standardized test scores. The FBI contends that administrators took a number of steps in order to ensure that scores met the federal standards set out by the No Child Left Behind law. It accused the indicted administrators and educators of denying enrollment to certain students, promoting or holding back students to influence test outcomes and of encouraging some students to enroll elsewhere outside of the district.
Four of those indicted had their bond set at $30,000, while one other had his set at $20,000. Earlier investigation into similar mail fraud allegations at the same Texas school district resulted in separate charges and convictions for two other educators, perhaps bringing to light the seriousness of the situation. Still, prior charges related to the same investigation do not establish guilt on behalf of any of the current defendants. All five are still presumed to be innocent with the right to defend themselves in such a manner that will achieve the best outcomes possible.
Source: khou.com, "5 educators charged in connection with Texas school cheating scandal", Aaron Martinez, April 27, 2016]]>
The lawyer that you choose can directly impact the criminal proceedings, including the amount of your bail and the time, if any, that you will spend in jail. Upon arrest or the moment that you learn you may be under investigation, it is important to seek the assistance of a lawyer who is experienced in criminal defense. Our close knowledge of the Texas Penal Code and the criminal justice system gives our clients a distinct advantage during a complex and difficult time.]]> We have experience defending Texas clients against state and federal charges. From drug charges to fraud, we know how to build a strong case, including investigating and collecting evidence for use in court. We also handle cases related to drunk driving charges, juvenile crimes, sexual offenses, domestic violence and more.
No matter the type or nature of the charges against you, you have rights that must be recognized and protected. With an experienced lawyer on your side, it may be possible to avoid or mitigate some of the penalties you face. The best way to ensure that your rights are protected and that you have a full understanding of your legal options is to meet with an attorney experienced in criminal defense.]]>
The 23-year-old university student was apparently on his way home from a party in the early hours of the morning. Police believe he was speeding when he attempted to pass another vehicle, taking his pickup truck onto the sidewalk rather than the road for which he had been aiming. A couple happened to be walking along the sidewalk at the time and were hit by the truck. The girlfriend survived with injuries, though her boyfriend was killed.]]> Authorities took a sample of the driver's blood shortly after taking him into custody. They also claim he admitted to drinking alcohol before the accident. The results of the blood test could potentially affect his charges depending on the resulting blood alcohol content. As it currently stands, his charge of intoxicated manslaughter could land him behind bars for 20 years.
There is no denying the seriousness of drunk driving related charges. Even misdemeanors can result in jail time and hefty fines, while virtually all those charged with being intoxicated behind the wheel stand to lose their driving privileges. Whether a charge is backed up by a definitive blood alcohol content level or not, all Texas defendants have the right to the presumption of their innocence in the eyes of the law. Simply being investigated for DUI or facing trial does not indicate guilt, which can only occur if a prosecutor is able to prove that the full extent of the charges are true beyond a reasonable doubt.
Source: chron.com, "UH student accused of drunken-driving fatality denied bail", Brian Rogers, April 20, 2016]]>
Miller not only serves as the agriculture commissioner but also works as a calf-roper in rodeos. Officials claim that he used at least $1,000 of tax payer money to travel to another state in order to receive an injection of an anti-inflammatory that is only available from one doctor in the United States. The shot has been dubbed "the Jesus shot" for its supposed effectiveness at treating chronic pain.]]> A report in the newspaper brought Miller's out-of-state trip public and an advocacy group followed up by filing a complaint against him. He reimbursed the state following the complaint. However, it is still not clear if he actually received the injection during that trip, and Miller has refused to comment on the matter. A representative for the commissioner stated that the investigation is not necessary and is indeed an overreaction to what he claims is an otherwise simple complaint that has already been addressed and handled appropriately.
Being the subject of an ongoing criminal investigation can be quite unnerving. Some individuals in Texas might postpone seeking appropriate legal assistance out of fear that doing so will make them appear guilty of committing a crime. Whether being investigated for a white collar crime, drug possession or any number of allegations, each and every individual accused or suspected of violating the law is still presumed innocent with full access to their rights during the course of the investigation and any subsequent criminal proceedings.
Source: morganton.com, "Correction: Texas Agriculture Chief-Investigation story", Paul J. Weber, April 14, 2016]]>
In March 2013, police seized what they suspected to be cocaine from a taxi cab that had been abandoned. After being transported to a police facility, officers discovered that 4 of the 17 kilograms of possible cocaine had been removed. Four wooden blocks were found mixed in with the drugs to apparently make up for the lost weight.]]> According to a defendant, who is cooperating with police, the former-officer was apparently involved with a drug trafficking network and helped stage the seizure. The cocaine obtained during the seizure was actually diluted with other substances, although it still held enough of the drug to test positive if investigators went that far. Investigators believe that the event was staged in order to conceal the subsequent distribution of the drugs.
The ex police officer was allegedly compensated with $5,000 for taking part in the setup and follow through of the seizure, although authorities have not yet named the other defendant who has been providing them with information. Drug charges at the federal level can have severe consequences, including steep fines and time spent behind bars. However, defense preparations for both federal and state crime charges are largely similar. In general, most defendants in Texas can determine the best approach by conducting a thorough and careful review of the charges alongside their respective counsels.
Source: lmtonline.com, "Ex-South Texas cop faces drug trafficking charges", Aaron Nelsen, April 4, 2016]]>
The 29-year veteran French teacher gave one of her students an average of 90 percent for the grading period in question, a grade that apparently kept the student from being listed as one of the top in the class. With an eye on a $40,000 scholarship that is only available to those who graduate among the top of their class, the student's parents met with the teacher and apparently insisted that she had been unfair. They also requested that the student's grade be increased from 90 to 100 percent.]]> When the teacher failed to yield to this request, the parents complained to the school principal, who is student's uncle. The principal and teacher met with one another, and he allegedly threatened the teacher if she refused to comply with the grade-change request. The teacher still refused, but the student's grade was still changed so that she was listed as one of the top students in her class. According to the teacher, the principal's secretary forged her signature in order to make the change.
Afterward, the teacher complained to the human resource department for the school district. A local officer launched an investigation into the claims and questioned the teacher several times, during which she repeatedly denied signing the form that approved the grade change. In the end, she was arrested and charged with filing a false report. She also did not receive an arraignment despite being booked and released from jail, leaving her initially confused as to why she was even arrested.
The teacher insists that her misdemeanor charge for filing a false report is the result of inappropriate action taken by the principal and the district in an effort to cover up unprofessional behavior. It can be extremely frustrating for Texas defendants when law enforcement does not appear to take their concerns and complaints seriously to such a point that criminal charges are then filed. Typically, responding to these accusations in a timely manner can create an opportunity in which to develop the best defense strategy possible.
Source: courthousenews.com, "Texas Teacher Calls Arrest Bogus Bullying", Cameron Langford, March 31, 2016]]>
The pilot was under investigation for 8 months, and police say that he was responsible for several brothels that fronted as simple massage parlors. Up to 10 women were apparently living in each one, for which they were expected to pay several hundred dollars a week in order to work. This money apparently covered the costs of online advertising.]]> Police also arrested 19 different women, although their charges and alleged roles in the matter is much less clear. However, a 37-year-old woman is believed to have helped the pilot manage everything in Houston. She later posted $5,000 bond and was released from police custody. The pilot also posted bond, although his was set at $15,000. United Airlines released a statement saying that it has since removed the pilot from his previous flying duties, but he is apparently still an ongoing employee while the investigation continues.
While some in Texas might call for the end of his employment with the airline, it is vital to remember that being accused of state crime charges is not the same as being convicted. At all points throughout the investigation and criminal court proceedings, defendants are still innocent under the law with full access to their rights and privileges. During this time, most defendants choose to carefully review their charges alongside their counsel in order to create the best defense strategy possible that will help them achieve the most favorable outcome for their personal situation.
Source: insideedition.com, "Cops: United Airlines Pilot Arrested For Running A 'Prostitution Enterprise'", Johanna Li, March 25, 2016]]>
It is not clear where the Lyft driver's passenger was heading, although he did disclose that he had sold his vehicle and regularly used Lyft as a means to travel to and from work. The passenger was under the impression that his driver might have simply been inattentive when he began to drift out of his lane. Later, he reported that the driver hit the brakes at a green light.]]> An officer initiated a traffic stop shortly after the braking incident. When the driver went to retrieve his proof of insurance, the passenger claimed that he could smell some type of alcohol on him. The attending officer then administered a field sobriety test, and although there was no official disclosure as to how he performed, his passenger did not think that it went well. The driver was arrested shortly afterward.
The Lyft driver was previously charged with a DWI in 2004, but those charges were later dropped. From the length of time that a license is suspended to fines and jail time, the impact of a conviction on a person's life can be serious and long-lasting. In order to minimize the possible impact, most defendants in Texas are well advised to begin criminal defense preparations in as timely a manner as possible.
Source: khou.com, "Lyft driver arrested for DWI with passenger in vehicle", Ashley Goudeau, March 15, 2016]]>
The 20-year-old junior serves as president for the Phi Delta Theta fraternity. According to police reports, the woman who reported the apparent assault was given an unidentified drink while at the party. After drinking it, she supposedly became disoriented and was led outside by the fraternity president under the guise of getting some fresh air. The assault then allegedly occurred in a secluded area outside the house, where the woman claims she lost consciousness at one point.]]> Upon coming to, the woman located a friend, and the pair went to the hospital. Although it is unclear exactly what transpired at the hospital, it is possible that a rape kit was performed. The police were then notified sometime after the woman arrived at the hospital. Charges were quickly filed, and the arrest followed soon. The quick response is possibly related to the hefty criticism that Baylor University - - the college that both the alleged victim and defendant attend - - has faced for previous handling of sexual assault claims.
Concern for both potential victims of sexual assault and their alleged attackers is understandable, especially when an institution has not been entirely forthright in the past when it comes to handling such sensitive subjects. However, overreach of that concern can be dangerous. Regardless of what might have occurred in Texas in the past, each and every defendant has the right to a fair trial where his or her rights are maintained upheld to the letter of the law.
Source: wacotrib.com, "Baylor fraternity president charged with sexual assault", Kristin Hoppa, March 3, 2016]]>


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I have a background primarily in Java, along with some experience using JavaScript for front-end work, but am interested in transitioning to a full-time Ruby position, I do have some experience working in Ruby professionally from a past job and have also worked on my own projects using Ruby on Rails, I would appreciate if anyone can pass along information about any companies that may be open to my background. My LinkedIn profile is: http://linkedin.c... Thanks!
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