text:
Estimation of data-specific constitutive exons with RNA-Seq data
Sequence analysis (methods)
RNA-Seq has the potential to answer many diverse and interesting questions about the inner workings of cells. Estimating changes in the overall transcription of a gene is not straightforward. Changes in overall gene transcription can easily be confounded with changes in exon usage which alter the lengths of transcripts produced by a gene. Measuring the expression of constitutive exons— exons which are consistently conserved after splicing— offers an unbiased estimation of the overall transcription of a gene.
We propose a clustering-based method, exClust, for estimating the exons that are consistently conserved after splicing in a given data set. These are considered as the exons which are "constitutive" in this data. The method utilises information from both annotation and the dataset of interest. The method is implemented in an openly available R function package, sydSeq.
When used on two real datasets exClust includes more than three times as many reads as the standard UI method, and improves concordance with qRT-PCR data. When compared to other methods, our method is shown to produce robust estimates of overall gene transcription.
The development of high throughput sequencing technologies has made it possible to sequence the transcriptome at a much higher resolution and coverage than was previously available. Sequencing of cDNA samples (RNA-Seq) has a dynamic range larger than that of microarrays
Alternative splicing
A gene is commonly seen as a fundamental unit in mRNA biology. While the term gene is commonly used, its usage and meaning has changed over time as our knowledge of the genome, its transcription and regulation has increased. We see it appropriate to use the definition that
Alternative splicing is a biological mechanism to expand the protein diversity from the limited gene pool
Alternative splicing needs to be be taken into consideration when analysing RNA-Seq data as it occurs ubiquitously within mammalian transcriptomes
Next generation sequencing
In the last decade, many studies of mRNA expression studies have been completed using microarray technology. Now there are many sequencing platforms including those of 454 Life Sciences, Illumina, Applied Biosystems SOLiD and Helicos Biosciences. There are many uses of these technologies, addressing various types of problems such as de novo genome sequencing, transcriptome sequencing, sequencing of microRNAs, chromatin immunoprecipitation sequencing
A typical RNA-seq data analysis workflow consists of many steps
Sequencing technologies produce reads of limited length, so each read is of a limited interval of a fragmented transcript. Sequencing only fragments of transcripts creates a significant bioinformatics burden in both the mapping and summarisation steps of the data analysis workflow. The longer an observed read, the higher the likelihood that it will span a splice junction. Identifying and aligning such reads is both computationally and statistically difficult as the number of possible splice junctions is large
There are many biological questions that may be addressed with RNA-seq data. A typical focus of RNA-seq analysis is to identify differential expressed transcripts or isoforms
Gene expression levels in RNA-seq experiments reflect the number of (or the amount) of mRNA that is within the samples. In a typical RNA-Seq experiment we can count the number of reads that map back to any given gene and associate this count with the amount of mRNA that gene produced. For a given gene, this read count is a function of the abundance of its transcripts in the cell and the length of those transcripts. Our main interest is in the abundance of transcripts created from a gene not the number of reads produced by gene. This subtle difference is driven by the fact that a longer isoform will produce more reads than a shorter isoform if both are expressed at the same abundance. Due to alternative splicing, a gene can produce isoforms of different lengths. Thus if the overall transcription of a gene does not change between conditions but the splicing does, this can result in a change of count (see Figure
Figure 1
Effect of differential alternative splicing on gene counts
Effect of differential alternative splicing on gene counts. In this toy example a gene with two isoforms is considered. Observing only the exon counts it may be reasonable to assume that sample one and two only contain transcripts from isoform one, while sample three only contains transcripts from isoform two. If the expression of a gene is measured as the sum of its exon counts then here sample three would generally be considered as differentially expressed from sample one or two. However, if this gene's expression were measured only using the counts from the first exon, this gene would not be considered differentially expressed. It would be reasonable to assume that samples one, two and three all contain a similar number of transcripts for this gene.
Estimation of constitutive exons
In order to focus on the overall expression of a gene, rather than isoform-specific expression, the Union-Intersection (UI)
● the annotation may include isoforms which are not present in the current samples, and
Figure 2
Processing exon annotation
Processing exon annotation. A graphic describing how the annotation of two overlapping genes is processed into an exon annotation appropriate for the use of exClust. The isoform annotation can be used to define a set of disjoint exon regions that could be rejoined to describe any of the known isoforms of the gene. It is these disjoint exon regions that are used as the exon annotation in exClust. Exon regions which overlap multiple genes are ignored. The set of UI exons are also shown for these two genes and are simply the exons that are present in all the annotated isoforms.
● the current samples may include isoforms which are not present in the annotation.
In the first case, the UI definition selects exons which are conserved across the isoforms present in the data but may exclude some exons which are also conserved across isoforms present in the data but not across all isoforms in the annotation. This is an issue as the number of reads summarised for a gene can affect the sensitivity of tests for differential expression
To address these issues we propose a new method, exClust, inspired by work on exon arrays
This paper develops a methodology for identifying the DSC exons within a gene between two or more conditions. These methods are then evaluated on two publicly available datasets
Processing exon annotation
We assume that, for the organism of interest, at least one set of transcript annotation exists (it may be derived de novo or a combination of multiple annotations) and that annotation source has been selected for use in the analysis. From this annotation, we define for each gene what we call
Estimate data-specific constitutive exons
Here G stands for gene, E for exon, T for treatment and S for sample. Exons are nested with genes, and samples within treatments. The variables
Assuming the count data,
The Anscombe transform will stabilise the variances if the data is Poisson and make
We expect the diagonal elements of
Following a similar method described for exon arrays
1. Apply Ward's linkage hierarchical clustering
2. Cut the clustering dendrogram, determining the cut-off height as below.
3. Evaluate all the resulting clusters using a scoring metric—again, see below.
4. Identify the cluster with the highest score. The exons in this cluster are the DSC exons for gene
This process is illustrated in Figure
Figure 3
Identifying constitutive exons
Identifying constitutive exons. Plot of exons selected by exClust for a particular gene. A clustering dedrogram of the exons is formed by apply Ward's linkage hierarchical clustering to the distance matrix
Deciding at what height to cut the clustering dendrogram is not a trivial choice. As we are analysing well annotated organisms we would like our method to perform similarly to the UI definition. To this end we choose to cut the dendrogram at a value that maximises the correlation of the exClust log fold changes with the UI log fold changes. A value of two maximised this correlation for the Bullard dataset following a grid search and may be a reasonable choice for poorly annotated data where a similar strategy would not be appropriate.
There are also many potential choices of scoring metric that could be used to select the subcluster of DSC exons. As DSC exons should be present in all isoforms of a gene, the DSC exons of a gene should hence have the highest number of reads mapping to them per base pair relative to the non DSC exons. Choosing the subcluster of exons with highest average coverage (the average number of reads mapped per base pair to each exon) may then be an appealing scoring metric. However this scoring metric can be affected detrimentally if a subcluster has a lowly expressed exon that was included by chance. An alternative scoring metric may then be to choose the subcluster that has the single exon with the highest coverage. However the efficiency of the sequencing and mapping process can be influenced by artefacts such as exon length, GC content or whether the exon is an initial, internal or terminal exon
Detection of differential alternative splicing
For the purpose of evaluating our method it would be useful to know if the relative abundances of gene isoforms has changed in two conditions. It is in this situation that comparing the overall expression of a gene in two conditions will be confounded by the changes in lengths of the isoforms. In comparisons across samples and/or conditions, it is standard to test for changes between the samples or conditions in total gene expression; that is, to test for "differential expression" of each gene. When we consider alternative splicing and the multiple isoforms this can produce, it is also of interest to test a gene for changes between the samples or conditions in the relative abundances of its isoforms. We will adopt the terminology used in
We will evaluate our method for identifying constitutive exons on two publicly available datasets (MAQC and Wang Data). These were chosen as both were well studied and clearly annotated. Both datasets have a relatively high amount of replication. The MAQC data also has qRT-PCR for a selected set of genes which aids in our evaluation by providing an accurate alternate estimation of gene expression.
MAQC data
The data consists of two mRNA-Seq datasets from the MicroArray Quality Control Project
Wang data
The Wang data
Evaluation study
In the following study we will primarily use the MAQC data to evaluate the effectiveness of our method for identifying constitutive exons. To do this, we will assess the concordance between the qRT-PCR data and the RNA-Seq data when summarising the RNA-Seq data using four different methods:
● Union the union of the exons,
● UI the UI definition
● Cufflinks sum of the FPKM values of all isoforms estimated by Cufflinks for each gene
● exClust the union of the exons selected by the clustering method.
The Union and exClust methods always select at least one exon for each gene. The UI method can fail to produce any exons, we refer to these genes as empty. In these cases no summarisation is possible. Log fold change values are calculated as follows. For each gene and summarisation method, when at least one exon is deemed to be constitutive, counts are summed over the set of selected exons and over replicates to produce a total count for each of the two tissue types. The log ratio between the totals for each tissue type is then used as the log fold change estimate for each gene and method. Any gene with a log fold change of positive or negative infinity for any method is ignored. Cufflinks was implemented following a standard pipeline
QRT-PCR is often considered a gold standard for gene expression measurement, even though it is highly reliant on primer choice. If the primer probes for the qRT-PCR data were generally chosen in DSC regions of the genes, we expect that a better summarisation method will show higher concordance with the qRT-PCR results. In particular, as the quantification of the qRT-PCR is independent of transcript lengths, a summarisation method that removes the bias of differing transcript lengths should offer improved concordance with the qRT-PCR data. We will use two criteria to assess this concordance. Both methods rely on the detection of differential alternatively spliced (DAS) genes. A gene will be called DAS if it has a Bonferroni corrected DASI p-value less than 0.05
Criterion 1: Log fold change values from the given method are regressed against corresponding qRT-PCR values. Residuals for all genes against this fitted line are then computed. The top 20 DAS genes are ordered by log qRT-PCR fold change, and their residuals are plotted. An effective summarisation method should be unaffected by the length bias produced from differential alternative splicing and hence changes in residuals should be seen with the Union summarisation for these DAS genes but not the UI and exClust summarisations.
Criterion 2: In this second criterion we compute the Pearson correlations between log fold change values from the Union, UI and exClust summarisations, the sum of the isoform FPKM of Cufflinks and the qRT-PCR value. This is done separately for
● the DAS genes, and
● the non-DAS genes,
where only genes with a non-empty UI definition are used. An effective method will produce a high Pearson correlation score in all cases.
For the Wang dataset, qRT-PCR data is not available. For this data set we computed Pearson correlations between the three summarisation methods.
Results and discussion
We developed a novel clusting algorithm, exClust, for the estimatation of data-specific constitutive exons and implement it in R language
We begin by examining the MAQC data. The Poisson assumption appears to hold for the data as seen in Additional file
● 62,850,300 reads for the Union summarisation,
● 49,191,469 for the exClust summarisation, and
● 15,249,893 for the UI summarisation.
Additional file 1
Includes additional figures demonstrating the validity of the Poisson assumption and the performance of UI and exClust on two genes.
Click here for file
There is a successive loss of reads as each method makes increasingly stricter assumptions.
Before we evaluate the effectiveness of these different summarisation methods, we examine the conceptual links between differential alternative splicing and differential expression. In Figure
Figure 4
Concordance plot
Concordance plot. Concordance plot with the RNA-Seq log fold changes on the y-axis and qRT-PCR log fold changes on the x-axis. For the RNA-Seq data we use the union of all exons within a gene to summarise our counts where a value of one is added to the count of every gene. The black circles are those genes for which the UI definition is non-empty. The blue triangles are the 386 genes for which the UI definition is empty. The red dots are those genes that our method identified as having a change in isoforms and had a non-empty UI definition.
In Figure
Figure 5
Residual plot
Residual plot. After fitting a straight line through the plot in Figure
We now consider Criterion 2. As quantification by qRT-PCR is independent of transcript lengths, a summarisation method that removes the bias due differing transcript lengths should offer improved correlation with the qRT-PCR data. A numerical summary of these correlations is presented in Table
Additional file
Similar outcomes were found with the Wang dataset. First there are large differences between the number of reads summarised by each method:
● 13,949,371 reads for Union summarisation,
● 10,892,133 for exClust summarisation, and
● 4,138,796 for UI summarisation.
Correlations for the three summarisation methods and Cufflinks can be found in Table
We have implemented the exclust method in the R language
When working at a gene level, between-treatment differential alternative splicing could cause problems with an expression analysis. The concept of constitutive exons helps to resolve these problems by finding exons which are common to all isoforms of a gene. We have proposed a novel approach to estimating the constitutive exons in a gene, using both empirical and annotated data. Importantly, we allow constitutive exons to be data-specific. That is, we define data-specific constitutive exons as exons which are common to all the isoforms of a gene which are present (in significant abundance)
For simplicity, in the development of these methods we have modelled the read count data using standard Poisson assumptions. While the technical variability between samples should be Poisson, most experiments have an element of biological variability as well and hence RNA-Seq data is often modelled as an overdispersed Poisson. Modelling this overdispersion is beyond the scope of this paper. A more sophisticated methodology would model this overdispersion and standardise accordingly
Our approach for empirically estimating the data-specific constitutive exons within a gene can be seen to perform favourably when compared with the current alternative. Our method provides the performance benefits of the UI definition without the dramatic decrease in total read count.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
EP developed the method, implemented the algorithm and drafted the manuscript. MB and YY participated in all aspects of the study and helped to draft the manuscript. All authors read and approved of the final manuscript.
The authors wish to thank Denis Bauer for her useful suggestions. This work was supported in part by ARC through grants FT0991918 (YY) and Australian Postgraduate Award (EP).
The publication costs for this article were funded by the corresponding author's institution.
RNA-Seq: a revolutionary tool for transcriptomics
Mapping and quantifying mammalian transcriptomes by RNA-Seq
What is a gene, post-ENCODE? History and updated definition
Activation and repression of gene expression by POU family transcription factors
Alternative pre-mRNA splicing and proteome expansion in metazoans
Alternative splicing of pre-mRNA: developmental consequences and mechanisms of regulation
Exonic splicing enhancers: mechanism of action, diversity and role in human genetic diseases
Mechanisms of alternative pre-messenger RNA splicing
Different levels of alternative splicing among eukaryotes
Genome-wide detection of alternative splicing in expressed sequences of human genes
Genome-wide survey of human alternative pre-mRNA splicing with exon junction microarrays
Initial sequencing and analysis of the human genome
Alternative isoform regulation in human tissue transcriptomes
From RNA-seq reads to differential expression results
Optimal spliced alignments of short sequence reads
TopHat: discovering splice junctions with RNA-Seq
Supersplat–spliced RNA-seq alignment
MapSplice: accurate mapping of RNA-seq reads for splice junction discovery
Statistical inferences for isoform expression in RNA-Seq
RNA-Seq gene expression estimation with read mapping uncertainty
Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation
Comparative systems biology of human and mouse as a tool to guide the modeling of human placental pathology
KEGG for integration and interpretation of large-scale molecular data sets
Evaluation of statistical methods for normalization and differential expression in mRNA-Seq experiments
Transcript length bias in RNA-seq data confounds systems biology
Probe selection and expression index computation of Affymetrix Exon Arrays
The MicroArray Quality Control (MAQC) project shows inter- and intraplatform reproducibility of gene expression measurements
A scaling normalization method for differential expression analysis of RNA-seq data
Effects of collapsing multidimensional contingency tables
The Transformation of Poisson, Binomial and Negative-Binomial Data
Hierarchical grouping to optimize an objective function
Modelling and simulating generic RNA-Seq experiments with the flux simulator
MADS: a new and improved method for analysis of differential alternative splicing by exon-tiling microarrays
Prediction of alternative isoforms from exon expression levels in RNA-Seq experiments
Ultrafast and memory-efficient alignment of short DNA sequences to the human genome
Ensembl 2009
Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks
edgeR: a Bioconductor package for differential expression analysis of digital gene expression data
Differential expression analysis for sequence count data


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South Glengarry to vote on sign language bylaw
The township of South Stormont passed a similar motion in September of this year.
The Canadian Press
Published Tuesday, November 13, 2012 11:47AM EST
Last Updated Tuesday, November 13, 2012 12:30PM EST
SOUTH GLENGARRY, Ont. -- The Township of South Glengarry will vote tonight on a bylaw allowing all businesses to put up signage in whatever language they chose.
The township east of Cornwall is following in the footsteps of another eastern Ontario municipality when it comes to language rights.
South Stormont passed a similar resolution in September.
The largely symbolic bylaw is meant as a push back to other municipalities passing laws requiring all businesses to have bilingual signage.
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The ADB Economics Working Paper Series is a forum for stimulating discussion and eliciting feedback on ongoing and recently completed research and policy studies undertaken by the Asian Development Bank (ADB) staff, consultants, or resource persons. The series deals with key economic and development problems, particularly those facing the Asia and Pacific region; as well as conceptual, analytical, or methodological issues relating to project/program economic analysis, and statistical data and measurement. The series aims to enhance the knowledge on Asia's development and policy challenges; strengthen analytical rigor and quality of ADB's country partnership strategies, and its subregional and country operations; and improve the quality and availability of statistical data and development indicators for monitoring development effectiveness.
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Apr 7, 15:40
The supporting technologies associated with energy storage systems include power conversion (primarily focused on inverters), system-level software and controls, and systems integration...
China 2014 PV export totals US$16.2 billion, says CCCME
Apr 7, 12:28
China-based PV makers recorded total export value of US$16.2 billion in 2014, with Japan accounting for 28% of the value, Europe 16%, the US 12%, India 3% and South Korea 2%, according...
Advanced distribution management systems expected to exceed US$3.3 billion in annual revenues by 2024, says firm
Apr 2, 13:55
Offering the potential to unify utility distribution IT systems into a single, more efficiently managed platform, advanced distribution management systems (ADMSs) have emerged over...
LG Chem to invest US$100 million to expand China polarizer facilities, says report
Mar 31, 20:43
LG Chem will reportedly invest US$100 million to expand polarizer facilities in China in view of growing demand for the technology in the nation as the LCD panel industry continues...
Smart building technology spending forecast to grow to US$17.4 billion in 2019, says IDC
Mar 30, 16:00
An increasing number of case studies are emerging to demonstrate the business case and benefits associated with developing smart buildings. According to a new IDC Energy Insights...
China-based MLS expects 2015 LED lighting revenues from US to hike 5-10 fold
Mar 30, 10:58
China-based LED packaging service provider MLS has extended production to own-brand LED lighting and began to sell LED light bulbs and tubes in the US market in 2014, and expects...
Global smart wearable entertainment devices and services market will top US$9.4 billion by 2019, says Technavio
Mar 27, 15:44
As the popularity of entertainment devices increases, the global smart wearable entertainment devices and services market is expected to grow at a CAGR of 37.09% from 2014-2019, according...
Epson estimates US$1 billion market for various printing technologies in 2015
Mar 27, 13:15
The printer market, consisting of inkjet printers in the residential and commercial segments, is expected to reach 70 million in shipments while laser printer shipments are estimated...
LED light bulb prices to fall to US$5 in 2015, says Epistar chairman
Mar 26, 15:07
The lowest price for an LED light bulb will drop from US$8 in 2014 to US$5 in 2015 and ex-factory prices will slip to US$2-3 in 2015, according to chairman Lee Biing-jye for Taiwan-based...
US fab tool book-to-bill ratio stays above parity, says SEMI
Mar 24, 21:02
The SEMI book-to-bill ratio for US manufacturers of semiconductor equipment came to 1.02 in February 2015, staying above parity for the second consecutive month.
Utility outage management system spending expected to total nearly US$11.8 billion from 2014-2023, says Navigant
Mar 24, 15:01
Historically, outage management systems (OMSs) have been configured to respond to signals that indicate outages, manage restoration, and communicate outage information. However, with...
Global single-axis tracker revenues expected to reach nearly US$2 billion in 2019, says IHS
Mar 24, 14:39
Global single-axis tracker revenues are expected to grow 120%, to reach nearly US$2 billion in 2019, according to IHS. For the first time, single-axis trackers will be the preferred...
Taiwan attracts foreign direct investment of nearly US$749 million in January-February, says MOEA
Mar 23, 11:53
Taiwan's Ministry of Economic Affairs (MOEA) approved 499 foreign direct investment projects (except from China) with a total value of US$748.85 million in January-February 2015,...
LED industrial lighting market to reach US$2.366 billion in 2015, says report
Mar 17, 15:47
The LED industrial lighting market scale will be worth US$2.366 billion in 2015, and by 2018 will reach US$3.935 billion, according to findings from LEDinside.
Solar cell pricing may drop if US finalizes reduced tariffs on China-made PV modules
Mar 12, 11:56
The US Department of Commerce earlier this year preliminarily lowered an average anti-dumping and anti-subsidization tariff rate imposed on China-made PV modules in 2012 to 17.5%...
Worldwide revenues from small and medium wind power systems expected to reach nearly US$2.4 billion by 2023, says firm
Mar 11, 15:05
Fueled by the spread of innovative financing programs centered around the leasing model, growth in the installation of small and medium wind power systems is accelerating. In the...
Inotera buys equipment from Hermes Microvision
Stockwatch - Mar 10, 20:22
DRAM chipmaker Inotera Memories has announced the acquisition of a batch of production equipment from Hermes Microvision (HMI) for NT$934.63 million (US$29.7 million).
Vehicle-grid integration service revenues expected to reach nearly US$21 million by 2024, says Navigant
Mar 10, 15:10
Today's plug-in electric vehicles (PEVs) represent a significant increase in electricity demand that, if unmanaged, could cause problems with distribution-level transformers and could...
Commercial building automation system revenues expected to total more than US$713 billion from 2015-2023, says Navigant
Mar 6, 14:11
The opportunities presented by analyzing the vast amounts of data that building automation systems (BASs) can collect are accelerating their adoption across the commercial building...
ASMedia appoints lawyers to defend patent lawsuit against VIA
Stockwatch - Mar 4, 22:16
Commenting on its lawsuit against VIA Technologies, which VIA has accused ASMedia for infringing its intellectual property rights concerning USB 3.0 in the US, ASMedia has issued...
Revenues from energy efficient building products and services expected to total more than US$4.3 billion from 2015-2023, says Navigant
Mar 4, 15:39
Current building stock accounts for a substantial amount of global energy use, putting increased pressure on governments and corporations alike to reduce their carbon footprints and...
- MediaTek rolls out 4G solutions for vehicle recorder market
- Largan expected to keep lead in smartphone dual-lens module
- Getac expects strong 2H12
- Neo Solar Power unveils 3 high-efficiency solar cell models
- Foxconn chairman expects channel retail business to grow strongly in 1 year
- CPT at full capacity with order visibility through 3Q16, says president
- Acer achieves breakthroughs in non-PC businesses, says chairman
- Taiwan sets 2025 target renewable energy installation capacity at 27.423GW
- Digitimes Research: Samsung Display, LG Display to keep expanding AMOLED capacities
- Chenbro to expand capacity to meet growing demand from data centers
- China makers count on government subsidies for profits, says report
- Shenzhen Stock Exchange questions NavInfo acquisition bid for MediaTek China unit
- Apple developing Micro LED panels for Apple Watch, say Taiwan makers
- Sonix 2Q16 revenues to rise 50-60%, says report
- Taiwan market: 641,000 handsets sold in May, says CNA
- Everlight plays down impact of Japan patent infringement ruling
- Digitimes Research: Tablet AP shipments in China to decrease 2.9% in 2016
- Canadian Solar, Flextronics to jointly set up PV module factory in Brazil, says Absolar
- Foxconn chairman looks at LeEco ecosystem for possible cooperation
- DoC imposes tariffs on China-made SolarCity PV modules
- Acer expects Europe operation to be affected by UK vote
- Power transistors to see less volatility in second half of decade, says IC Insights
- Taiwan market: APT to launch VoWiFi services
- Compal chairman expects Taiwan exports to benefit from rising Japanese Yen
- Beijing to build new IC industrial park with total investment of CNY4 billion, says report
- Releases
- White papers
- Bulletin
- Taiwan Pavilion features innovative smart Taiwan at MWC Shanghai
- Open innovation platform helps shape the landscape of IoT applications
- Institute for information industry and open connectivity foundation join forces to enable global IoT market opportunities for Taiwan-based vendors
- USB-IF promotes USB Type-C connectors to enhance performance and user experience
- CSO: Heavy duty fuse clip designed for voltages up to 1500 VAC / VDC and currents to 32 A
- Unisys IT Implementation of Unisys Stealth Solution for Data Center Segmentation
- AccuTouch Five Wire Resistive Touchscreens
- Getting the Most Out Of Your Next Generation Digital Security Surveillance System
- Embracing the New Generation Intel Atom Family with DDR3 Memory Support
- Got The Message? Ensuring efficient and reliable delivery of content across a mission critical digital signage network
- Perforce Software Wins Develop Industry Excellence Award for the Second Year in a Row
- Perforce Teams with Leading Git Client Developer fournova
- Perforce Introduces Helix GitSwarm: Flexible Git Ecosystem Mirrored to a Powerful Mainline Repository
- Perforce Helix Distributed Version Control System Brings Greater Flexibility While Maintaining Enterprise-class Security, Auditability and Control
- Perforce Powers Development of Legacy Quest for iOS and Android
24-Jun-2016 markets closed
|TSE electronic
|OTC electronic
- Brexit: UK votes to leave EU in historic referendum (Jun 24) - BBC News
- Why you should care about the Brexit vote (Jun 21) - CNNMoney
- Tesla makes offer to acquire SolarCity (Jun 21) - Company release
- Ericsson faces US corruption probe: Swedish newspaper (Jun 16) - Reuters
- Counterfeits often better quality, says Alibaba's Jack Ma (Jun 15) - BBC News
- Siemens CEO says not interested in robot maker Kuka (Jun 12) - Reuters
- Foreign investors unload Japanese stocks as disenchantment grows (Jun 9) - Wall Street Journal
- Chinese fund buys Italian robot toolmaker (Jun 12) - The Financial Times
- Digitimes Research: 4 million 9.7-inch iPad Pros to ship in 1H16
- Digitimes Research: Global all-in-one PC shipments to drop 3.9% on year in 2015
- Mentor Graphics Veloce VirtuaLAB adds protocols for networking designs
- Mouser Electronics undergoing expansion
- Digitimes Research: iPad Pro shipments to be less than 3 million units in 4Q15
The gaming notebook competition: Q&A with Jone Chang from Gigabyte notebook department
Gaming notebooks have become the main focus of Gigabyte Technology's notebook department and despite...
Powering the cloud: Intel embraces new identity
The next wave of computing is now beginning to define the IT industry, where the boundaries of computing...
A new cycle begins for desktop motherboards: Q&A with Gigabyte technical marketing manager, Leon Chen
The launch of virtual reality headsets for the consumer market and the increasing demand for ultra...
IoT, Big Data and biological sciences: Q&A with Dassault Systemes president Bernard Charles
Dassault Systemes, a European multinational software company that develops 3D design, 3D engineering,...
- Smartphone and tablet industry: The view from Taiwan - May 2012
Taiwan-based companies have a long and successful history of involvement in the supply chain for mobile devices, which for the purposes of this article consist...
- Taiwan motherboard industry overview - May 2012
The year 2011 saw the motherboard industry reverse its trend of declining shipments year-on-year, to post a slight 0.2% growth. This upturn in fortunes was expected...
- Notebook market overview - Jun 2011
The notebook industry in 2010 experienced several major events that significantly changed the industry's ecosystem for the year and the industry is also believed...
- LCD TV overview - Jun 2011
Growth of the global LCD TV market was not as strong as expected in 2010 as the market continued to feel the aftershocks of the economic recession that had hit...
- Wireless broadband developments in Southeast Asia markets
As of 2013, the 10 ASEAN nations had a total of over 700 million mobile subscriptions, with the CAGR from 2003-2013 reaching 24%. This Digitimes Research Special Report analyzes the various mobile broadband markets in ASEAN and looks at the respective trends in 4G LTE development for those markets.
- 2015 global tablet demand forecast
This Digitimes Research Special Report provides a 2015 forecast for the global tablet market and analyzes the strategies of key market players such as Google, Apple, Intel, and Microsoft.
- 2015 China smartphone panel trend forecast
This Digitimes Research Special Report analyzes the strategies of key China-based major panel makers BOE, Tianma and IVO for attacking the different market segments through technology and pricing, and their relationship to local vendors Huawei, Lenovo, ZTE, Xiaomi and Coolpad.


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There are no Upcoming Shows listed for this artist.
Dan Frigolette
Dan Frigolette seems to be infiltrating the industry in a unique way, by his own hand. A self-starter with a plethora of impressive resume bullets, Frigolette is slowly working his way into our hearts and televisions.
Frigolette was recently featured in Time Out NY garnishing the coveted "Joke of the Week" honors. His appearances across comedy clubs in NYC initially caught the attention of Stonehenge LLC, a multi-billion dollar real estate firm based in New York, whose relationship spawned the comedic webicom The Apartmentship which stars Frigolette who co-wrote and co-produced alongside friend and fellow comedian Andrew Schulz from MTV2's Guy Code.
An avid writer with a Journalism Degree from Syracuse University, Frigolette has used his background to promote many long running shows in Manhattan and outlying boroughs. The creator of the Hoboken Comedy Festival which celebrated its 3rd successful year in 2012 helped to: spread comedy into Sinatra's place of birth, support local charities, and expand the careers of burgeoning comedians; Frigolette loves honoring the town where he currently resides.
Originally hailing from Syracuse NY, Dan's stage prose is energetic, full of story telling cues, and an honest depiction of his intricate neuroses. A consummate professional mixing between sharp crowd work and clever joke writing. Frigolette gets around the country doing tours and colleges all throughout the east coast, consistently frequenting Central New York to bring love and laughs to those who watched him become a man.
Dan Frigolette's Upcoming Events
Dan Frigolette's Videos
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Tweet Feed
- RT @traecrowder: NYC we will be at @GothamComedy on June 30 and need your help spreading the word! @averagedrew @coreyfcomedy Tix: https:… Jun 22
- #NYC! This Thurs 6/23 is the last episode of the season #axsgotham @AXSTV w/ @chrisdelia hosting! Get tix NOW bit.ly/28R9ry9 Jun 22
- RT @AXSTV: It's been quite a season of @GothamComedy. This week @chrisdelia hosts an All New episode! amp.twimg.com/v/a4803b33-f67… Jun 21
- RT @JessimaePeluso: NYC-Come to my fundraiser 2MORO @GothamComedy 6/21 6pm 💋ALL proceeds got to my friend Leah who's terminal w/cancer! htt… Jun 20
- On 7/3 come see Quincy Jones @TheEllenShow @HBO! A gr8 nite of comedy not 2 B missed. Tix @ bit.ly/2612jaG pic.twitter.com/HYz5FFZaPS Jun 16


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Oracle To Support 'Asianux,' But Not UnitedLinux
Another Vendor Turns From UnitedLinux
Oracle Corp. plans to support only three Linux distributions right now. The trio includes Red Hat; Novell's recently acquired SUSE Linux; and "Asianux," a new distribution being co-developed by Oracle, Red Flag of China, and Miracle Linux, Oracle's own Japanese-based subsidiary.
Oracle has no intentions of supporting Turbolinux, a founding member of UnitedLinux that is also based in Japan, according to Wim Coekaerts, principal of Oracle's Linux Kernel Group,
Red Flag and Miracle Linux have been combining their development operations, making it easier for Oracle to collaborate with Asia-based developers, Coekaerts noted, during an interview at LinuxWorld.
The development efforts are going on at Oracle's China Development Center in Beijing, China. Previously, the Miracle Linux developers worked at a facility in Japan. Oracle holds 54 percent ownership in Miracle Linux.
Why isn't Oracle supporting Turbolinux? "The Turbolinux distribution is UnitedLinux 1.0, and Oracle is not supporting UnitedLinux 1.0," Coekaerts contended. Other founding members of the currently floundering UnitedLinux organization included SUSE, SCO, and Latin America-based Conectiva.
Coekaerts also maintained that Oracle doesn't want to support more than three or four Linux distributions. "We're not going to be supporting 20 different distributions. That'd be too many."
Meanwhile, about 5,000 of Oracle's 8,000 developers are now doing their day-to-day work directly on the Linux platform. Oracle is on an "aggressive track" for migrating the remaining developers to Linux, according to Coekaerts.
"Linux will make a huge jump in 2004," predicted Coekaerts. Now that the 2.6 Linux kernel is out the door, he said, Linux kernel developers are starting to discuss possible enhancements to desktop Linux for the next kernel, which might be dubbed either 2.7 or 3.0. Some of the talk centers on VideoDirect.
- 1Linux Top 3: Alpine Linux 3.4, deepin 15.2 and Linux Lite 3.0
- 2Linux 4.7 Set to Boost Live Patching, Security and Power Management
- 3Linux 4.6 Charred Weasel adds USB 3.1 Support
- 4Linux Top 3: OpenIndiana 2016.04, Ubuntu 16.04 and Debian's New Leader
- 5Linux Top 3: KaOS 2016.04, TurnKey 14.1 and pfSense 2.3


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Raz's Midnight Macabre Horror Review: Wrong Turn 6 – Last Resort
An inheritance leads a young man and his friends to an abandoned resort inhabited by two sketchy caretakers and a clan of mutant cannibals. By now you wondered why the series was still going. By now the well had seriously run dry and the only curiosity you had about it was what the hell they…Read More
1996 brought us some great movies. Independence Day, Twister, Jerry Maguire, The Rock, and Mars Attacks! 2016 brought us some great movies too. Deadpool, Captain America: Civil War, Eddie the Eagle. Oh yeah, a sequel to Independence Day came out as well. But that doesn't make the list of great movies. Here's my review. If any of you listened to episode 2 of my podcast Future Flicks…Read More
"Pixelated Memories" – Issue 5 – SWN Radio
The 5th Issue of the SomewhatNerdy Radio Podcast "Pixelated Memories!" In this week's Nerd Talk we have a talk about our earliest video memories. In this Issue's news, we talk about the new Netflix Voltron series and tons of news from E3! You can find and listen to Future Flicks with Billiam right here –…Read More
Raz's Midnight Macabre Horror Review: Wrong Turn 5 – Bloodlines
A group of college students, on a trip to the Mountain Man Festival on Halloween in West Virginia, encounter a clan of cannibals. After the fun of number four it didn't seem to be a bad idea to try again with another. Unfortunately after all the entertainment we had in "Wrong Turn 4" seemed to be the…Read More
Raz's Midnight Macabre Horror Review: Wrong Turn 4 – Bloody Beginnings
A group of college students gets lost in a storm during their snowmobiling trip and takes shelter in an abandoned sanitarium which, unbeknown to them, is home to three deformed cannibals. When all else fails, prequel. In all honesty what more could you do at this point, after the failure of both sequels the studio had…Read More
Raz's Midnight Macabre Horror Review: Wrong Turn 3 – Left for Dead
When their transfer bus crashes in a West Virginia forest, a group of convicts and a corrections officer meet a rafter who is on the run from cannibalistic hillbillies who have murdered her friends. After the lackluster sequel this third entry wasn't exactly highly anticipated. It was highly obvious from the trailer for this third…Read More
Ep. 2 – Future Flicks With Billiam
In this episode of Future Flicks, I give you some news and talk about all the movies coming out the week of June 24th. There's a sequel to a 90's movie in the mix, a documentary about a heavy metal band made up of 13 year olds, and a movie about wiener dogs. Also happy…Read More
Raz's Midnight Macabre Horror Review: Wrong Turn 2 – Dead End
A group of reality show contestants find themselves fighting for their survival against a family of hideously deformed inbred cannibals who plan to ruthlessly butcher them all. The first film was a massive hit, it was more a shock that it took so long for a sequel to arrive. I was a huge fan of…Read More
Raz's Midnight Macabre Horror Review: Wrong Turn
Six people find themselves trapped in the woods of West Virginia, hunted down by cannibalistic mountain men. You voted for it, you got it! This should be fun! I remember Horror fans getting quite excited for this release with comparisons being drawn with the likes of "The Hills Have Eyes" and "Texas Chainsaw". In a…Read More
SomewhatNerdy Radio Podcast – Toy Promo
A quick look at our Nerd Talk topic for SomewhatNerdy Radio Podcast Issue 4!!! Issue 5 comes out very soon. You can listen and download the Podcast on iTunes HERE, on the Sticher app HERE or right on Soundcloud HERE. You got questions or comments about the show? Feel free to email us at firstname.lastname@example.com….Read More
CI Jump Street
A few weeks ago on my Future Flicks blog, I had Teenage Mutant Ninja Turtles: Out of the Shadows as my pick of the week. That turned out to be the wrong choice and it was a terrible movie. My pick last week had been The Conjuring 2 which I still haven't been able to see. This weeks…Read More


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Marcia Stefanick, PhD
Director, WSDM
Sonoo Thadaney
Managing Director, WSDM
Terri Oppelt ("TO") Preising
Program Associate, WSDM
Marcia Stefanick, Susan Love, and Lynn Westphal
Women's Health Forum 2013
Welcome to the Stanford WSDM Center ("wisdom"), the Stanford Center for Women and Sex Differences in Medicine
WSDM aims to expand our knowledge of women's health and sex differences in biology and medicine, from conception through the lifespan, in every medical discipline, from basic science to clinical research and population health science.
2015 WSDM Seed Grants Awarded
Congratulations to the following investigators whose proposals received $20,000 each in seed grant funds from WSDM on December 18, 2015:
"Feasibility of Detecting Changes in Bone Microarchitecture and Body Composition from Cross Sex Hormone Therapy (CSHT) in Transgender Youth" Tandy Aye, MD, Assistant Professor, Pediatrics; Mary Leonard, MD, Professor, Medicine
"Reducing Reactive Aldehyde Levels to Treat Endometriosis-associated Pain" Eric Gross, MD, PhD, Assistant Professor, Anesthesiology; Stacy McAllister, PhD, Post-doctoral Scholar, Anesthesiology; Daria Mochly-Rosen, PhD, Professor, Chemical Systems Biology
"Defining the Molecular Pathways Responsible for Female-biased Airway Disregulation in Chronic Asthma" Maya E. Kumar, PhD, Instructor, Medicine; Pehr Harbur, PhD, Professor, Biochemistry
"Examining Sex Differences in the Human and Mouse Placental Transcriptome During a Vulnerable Neurodevelopmental Window" Theo D. Palmer, PhD, Associate Professor, Neurosurgery; Virginia D. Winn, MD, PhD, Associate Professor, Ob/Gyn; Amy Braun (Doctoral Student), Neurobiology
"Sex Differences in Macrophage Activation: Role of miR-204" Philip S. Tsao, PhD, Professor, Medicine (CV Med); Nicholas Leeper, MD, Associate Professor, Surgery
Upcoming WSDM Events
WISER Community Summer Event
WSDM is introducing a new initiative in 2016: the WISER Community (WSDM Interdisciplinary Science, Education, and Research). If you are interested in learning more about the WISER Community and its upcoming meetings and events, join our listserv.
Seed Grant Workshop 2016
Details will be available soon for the 6th annual WSDM Seed Grant Workshop-stay tuned.
Recent WSDM Events
7th Annual WSDM Women's Health Forum 2016
On May 14, 2016, WSDM partnered with Stanford Health Matters to host the Health Matters|WSDM Women's Health Forum 2016, which featured experts from the School of Medicine presenting on issues affecting women in cardiology, cancer, dermatology, sleep, and more. Attendance this year was a record high for both. Watch the videos of the WSDM Women's Health Talks. Many of the other Health Matters talks are available on the Health Matters website.
6th Annual Basic & Translational Research Symposium on Sex Differences in Cardiovascular Health and Disease
February 24, 2016 WSDM hosted its 6th Annual Basic & Translational Research Symposium on Sex Differences. The focus was on Cardiovascular Health and Disease, and was very well received by a capacity crowd in LKSC Berg Hall. Experts from cohosts the Stanford Cardiovascular Institute and Women's Heart Health at Stanford presented their cutting edge research. Read the Stanford Medicine SCOPE blog on the symposium here. Check out the videos of the presentations on our WSDM YouTube channel.
WSDM Seed Grant Workshop
October 23, 2015 WSDM hosted its third seed grant workshop. The event featured updates from past seed grant award winners. The group identified questions regarding sex/gender and women's health that need more study and facilitated collaborations across disciplines.
WSDM Leadership in the News
The August 22 issue of Lancet featured a profile of WSDM Director Maria Stefanick and her career promoting the study of sex differences. Read the article here.
In the April 28 issue of the Proceedings of the National Academy of Science (PNAS), Dr. Stefanick, WSDM Advisory board member Londa Schiebinger, and other experts discuss the importance of sex inclusion in basic research and the implications of the NIH's May 2014 policy change to require a balance of male and female cells and animals in studies. Click here to read the article.
Associate Professor Michael Greicius, a WSDM Advisory Board member, was featured on National Public Radio's "Science Friday" discussing his research regarding women and Alzheimer's Disease. Listen to the audio here.
Stanford WSDM Events Calendar
WSDM Partners
Social media
Like us on Facebook for updates on events, news, videos and more! Stanford WSDM Center
Visit our YouTube Channel: "WSDMatStanford" for talks and lectures on the wisdom of WSDM!


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5 Things To Know This AM
Karlie Kloss will have you wishin' for spring with her latest Lancaster campaign. (NY Daily News)
Turns out being 35 and single is less Sex and The City and more Eat, Pray, Love. (The Frisky)
Finally, a holiday-movie-themed outfit guide that doesn't involve A Christmas Story. (Glo)
Karl Lagerfeld might be showing in Dallas because he loves it, but Chanel's Texas history runs deeper than we think. (The Style Con)
Rejoice, for Apple is giving away a boatload of free tunes, flicks, and apps this (post) holiday season. (GeekSugar)
- Politics | Karlie Kloss Lancaster Paris Spring 2014 Campaign
More from Politics
Michelle Byrom, After 15 Years On Death Row
Update: You can also listen to Michelle Byrom's story on the newest episode of Refinery29's podcast, Strong Opinions Loosely Held. We've embedded it at the...
Vanessa Golembewski
May 31, 2016
Election 2016
What Celebs Think About Gun Issues
Gun control is a contentious issue in the United States. Mass shootings provoke conversations about whether the country should have tighter gun laws, but nothing seems to change. In a presidential election year, though, an issue like gun control is even more important. In Refinery29's Vote Your Values poll, conducted with...
Meghan DeMaria
Jun 23, 2016


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Flyer on the Wall
¡Adios, Culeros!
Looks like a Man About a Horse will be a-ridin' into the sunset on Saturday, Nov. 7. Also in the saddle for the farewell show are COLOR WAR (Brooklyn) and Sam Irons and the Blank Stares (Burque), all at Burt's Tiki Lounge (313 Gold SW). Free, 21+. (Laura Marrich)
Join the FOTW Poster Posse! Submit your flyers at least one week in advance to alibi.com/fotw.
Today's Events
James McMurtry • singer-songwriter at Dirty Bourbon
Metalachi • heavy metal, mariachi • Goddamn Son of A Bitch at LaunchpadMore Recommended Events ››
tartar control and more • 7.20.2016
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"It's not essential to have read the first book to enjoy this one, but those who enjoyed the witty wordplay and whacked-out humor of The Last Dragonslayer will find the same delights in this sequel." — Publishers Weekly, starred review
"Imaginatively energetic and made more so by a gloriously formidable female protagonist."
— The Horn Book Magazine
"The second volume in The Chronicles of Kazam is just as full of inspired silliness as the first . . . Fans will cheer this new installment, while readers who are new to the series can leap right into the fun."
"Jennifer is a fabulous character-smart, resilient, brave, willing to speak her mind and follow her heart, even when it absolutely does not seem like the right thing to do. . . The pace rarely slows. Nor does the silliness."
—School Library Journal


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Juventus vs Sampdoria Soccer Head to Head Statistics and Results
Head to Head Statistics : Juventus vs Sampdoria Year up to 2016The Soccer Teams Juventus and Sampdoria played 22 Games up to today.
In this common Games the Teams scored a average of 2.7 Goals per Match.
Juventus won all recent 7 Home Matches of the League in Streaks.
Overall Sampdoria lost all recent 3 Games in Streaks.
To comparison, on all remaining Matches against other Teams Juventus
made a average of 2 Home Goals Tore per Match and Team Sampdoria 1 away Goals per Match.
Detailed Result Comparisons, Form and Estimations can be found in the Team and League Statistics.
More H2H Statistics for this two Soccer Teams :
H2H Comparison Juventus - Sampdoria
H2H Goal Comparison Juventus - Sampdoria
Serie A Betting Tips and Predictions
Bet on next Soccer Matches live at Tipico and get a
100% Betting Bonus on first Deposit up to 100 € now. Tipico Bonus
Details for Tipico and more of best Bonus offers.
Results Juventus and Sampdoria
Cross Comparison Juventus and SampdoriaDirect Comparison of the Matches for Juventus and Sampdoria related
the recent Games and their Results of both Teams against common opponents.
Schedules Juventus and Sampdoria
The Soccer H2H Statistics updated on 27/06/2016 at 15:00.
Current Soccer live Results, Scores and Schedules for Juventus and Sampdoria
are also in the WP Result Service : Results Soccer and in the Schedules : Schedules Soccer.


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The Wintec Chafeless Girth has a non-chafe design and hidden elastic for horse comfort. A wipe-clean, sweat-resistant exterior makes taking care of this girth easy. A great value!
Keep your horse cooler and chafe-free with the Lettia Coolmax® Fleece Girth. Coolmax lining for breathability. Patented Clik buckles slide easily and smoothly for easy girthing.
The Tory Leather Neoprene Training Surcingle is so easy to clean that you can just hose it off. This practical surcingle has multiple ring placements and adjusts from both sides with a range of 66" to 76" overall.
Used by the best in Europe, Vienna Lunge Reins are gaining popularity in the U.S. They encourage the horse to round and release his back, working in a classically correct manner to develop properly.
Designed to our specifications, Dover's Training Surcingle has D-rings at appropriate points to allow the flexible use of training aids. Our surcingle is made of high-quality webbing with leather pads and reinforcements.
This quality Classic Leather Halter is triple stitched and made of soft and durable leather with a double buckle crown. Features an adjustable chin, rolled throat with snap and solid brass hardware.
The Medallion Super-Quilt® High-Profile saddle pad stays above the withers to provide a comfortable fit for your horse. Quilted non-slip 100% cotton underside keeps your horse cool. This pad seamlessly takes you from the schooling ring to the show ring.
Ideal for training, performance or competition, the Professional's Choice® SMX Girth is non-chafing, flexible and slip-resistant, making it ideal for horses with sensitive skin. The neoprene material is durable and can be washed with soap and water. A great buy!
The Professional's Choice® SMX Dressage Girth is an advanced girth featuring 4-way stretch neoprene as a shock absorber. A durable girth that is ideal for training, performance or competition.
The holes on the Collegiate® Half Hole Leathers are spaced 1/2" apart to provide a precise adjustment. These quality leathers perform well and look great.
This Crown Plain Raised Standing Martingale has a raised body strap and flat neck strap. This durable martingale made from quality leather is attractive and functional for schooling and showing.
Representing great value and a classic look, the Suffolk™ Dressage Bridle has a padded, raised flash caveson and brow. It's made of quality imported leather and comes with web reins.
Crafted of 3/4" wide leather, Tory Leather Dog Bone Side Reins adjust from 40" to 44" and attach with trigger snap ends. Rubber dog bone insert allows give.
Made of quality, easy-care materials, this Shires SupaFleece Half Pad has a classic look. The quilted top is paired with a luxurious synthetic fleece lining and edging that looks and feels like real sheepskin. It has a contoured cut and touch-close straps.
Dover's Shipping Boots combine great quality with a great price. This set of four economical shipping boots features durable outers, nylon linings and easily adjustable hook-and-loop closures. Protects your horse's legs from the knees and hocks to the heels and coronary bands.
Protect your horse's lower legs in the schooling ring or pasture with Dressage Sport Boots2. These horse boots are lined with fleece for comfort and feature a tough, Coverlight® shell with comfortable foam padding. Pair.
From Happy Mouth Bits®, the Jointed Pelham Bit features soft, flexible, durable, apple-scented plastic to encourage bit acceptance and softness.
This Lettia Clik Dressage girth is easy to adjust even when mounted. It has a chafeless, contoured design with a gel insert and elastic ends for horse comfort.
Professional's Choice® Ventech™ Dressage Girth features breathable neoprene. Helps girth area stay cool and comfortable. Non slip.


text:
Mobile price-war benefits customers
Relevant offers
National business
A mini price-war has broken out in the mobile market as the country's three mobile network operators offer sharper deals to pre-pay customers willing to make an upfront spending commitment.
Vodafone and 2degrees are both offering $19 monthly bundles for a limited time that provide 50 minutes of calls, 5000 texts and 500 megabytes of mobile data.
Telecom is offering the same deal at the same price, but with an extra 10 minutes of call-time thrown in.
Its discount Skinny brand improved its pricing yesterday, offering 60 minutes of calls, another 60 minutes of Skinny-to-Skinny calls, 100 texts and 60 megabytes of mobile data priced at $4 a week.
Skinny manager Paul Taylor said the market had gone "gangbusters" in the past week. "The value propositions are a lot stronger."
Telecommunications Users Association chief executive Paul Brislen agreed the deals were good and said he hoped they were here to stay.
Customers could in large part thank 2degrees for bringing more competition into the pre-pay market, he said.
"They have been so successful at this level of the market that the other telcos simply could not ignore it. The customers are the winners because suddenly we have got three or four really competitive offers there."
He hoped the improved offers would spread to the on-account and business market.
- BusinessDay.co.nz
Sponsored links
Whanganui tops first-home affordability ratings
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New global help desk for technology sector could create 300 jobs in Wellington
Laura Ashley closes NZ stores, owes creditors $2.3m
Combined effort to boost Manawatu's migrant workforce
Stuff is turning 16, and so is Ben
New era for Lotus in New Zealand and Australia
Fellow Christchurch developer goes into bat for $50m McKenzie and Willis development
Pattrick Smellie: Politics in an age of deep uncertainty
Crown goes it alone on Christchurch Convention Centre after breakdown
Waikato pushes for water council-controlled organisation, waits for friends to join the party
New Zealand team teaches Afghan villagers to manage their own electricity
Marlborough Civic Theatre Trust wants more council support for performing arts
Kiwis caught up in Ikea funiture recall after six children crushed
Police investigating claims unnecessary force was used in arrest of man hard of hearing
Electronic combat competition sets up for battle in New Plymouth
Giant puffball found near New Plymouth
Humpback whale pod spotted off Opunake coast
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Google turns NZ streets into a giant game of Pac-Man
'Dare to believe' speech aids Taranaki bowlers
Upsets in opening round of club rugby
Chaos the dog is back from the brink
Losing hair supporting loved-ones
South Taranki iwi receives Crown apology
Taranaki paracyclist chops wood to raise funds
Petitioner says DHB has 'ripped' out service
Police investigating claims unnecessary force was used in arrest of man hard of hearing
Giant puffball found near New Plymouth
Facebook increasingly a part of the Taranaki criminal's toolkit
Humpback whale pod spotted off Opunake coast
Electronic combat competition sets up for battle in New Plymouth
New Plymouth gets its first disk golf course at Merrilands Domain
Tasman Toyota's motor vehicle dealership celebrates 70 years
Ryan McCone keen to add his experience to CD bowling lineup
Chiton-crab a rare find in Taranaki
Convicted murderer Denis Luke's family appeal his release rejection
Shortage of women and young people standing in elections in Taranaki


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Appliances, cabinets pilfered from new homes
Irvine police are investigating four burglaries carried out between Jan. 25 and Jan. 27 in which thieves stole $5,600 worth of items from new, unoccupied houses.
Police said the houses are located along Portola Parkway between Jeffrey Road and Portola Springs; they were entered through unlocked doors and through doors that had their hinges removed by the thieves.
Two of the homes were stripped of new microwaves and refrigerators. Another was missing $300 worth of door hardware. In one case, a set of cabinets worth $5,000 was stolen.
Police said burglars struck six other times in the area earlier in the month. Other thefts also involved building materials such as flooring and tools.
Detectives are investigating the thefts and the possibility of their being related.
Anyone with information about the thefts is asked to call Irvine police at 949-724-7000.
Contact the writer: 714-796-7868
Contact the writer: email@example.com
Contact the writer: Twitter:@earleyOC
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text:
Jayme Moffatt and her dad, Chuck Saarlos, wiped away tears from their eyes as they watched the evergreen tree transform magically into a Tree of Lights.
Manteca filmmaker Jon Walkup credits his time in the video production class at Sierra High School – where he graduated from last year – as providing him with the supportive environment he needed to tackle his artistic endeavors.
The Manteca Pumpkin Fair folks have turned 30.
RIPON – For more than a decade the annual 'Taste of Ripon' has allowed residents to mingle with downtown merchants in a friendly and festive environment.
POPE VALLEY - Budge Brown spent 31 seasons spreading joy among hundreds of thousands of people who stopped by the Manteca Waterslides.
Sales tax collected within California's 768 local jurisdictions - including Manteca - were mostly flat for the July, September, and August.
A Doctors Hospital Christmas tree representing yuletide donations to the Hospice of San Joaquin will burst into a colorful and brilliant dedication tonight at 6 p.m. to loved ones who have passed away.
Kassy Menke found herself in the role of the bully.
Manuel Medeiros is a unique breed of politician.
The biggest thing in Manteca this time of year isn't the twilight parade, homes with 130,000-plus lights, or even Santa's arrival at his downtown hut.
There are 10 bands, hundreds of smiling kids doing everything from twirling to dancing, and fire engines bringing up the rear carrying the guest of honor.
RIPON – Keith Moore stood patiently alongside the gazebo at the Mistlin Fountain Monday night and waited for the Christmas tree lighting festivities to begin.
LATHROP - One can't talk about Lathrop's economic growth in the last decade or so without mentioning the non-profit San Joaquin Partnership at the same time.
The sounds of the season will fill churches and schools alike starting this week.
LATHROP - One of the biggest public works projects in the South County in 2011won't be undertaken or funded by any city.
Page 614 of 883
One of the three remaining undeveloped parcels in the 360-acre Spreckels Park project may soon have a 285,215-square-foot building for industrial uses.
Some 107 years after South County residents embraced the then bold move to form the South San Joaquin Irrigation District, secure what are now senior ...
Bill Cabral - a man who parlayed a trust in people, an outgoing personality, and a work ethic second to none to found Cabral Motors in ...
Is it really in the public's best interest to have police dispatch calls unencrypted so anybody can hear them?
The Ripon Unified school board will meet Monday in part to pass the proposed budget for the 2016-17 school year.
A Night of Hope is coming to Manteca.
Missed opportunities.
There was a time when a parent could take their child to the Library Park playground during the day and if he or she needed ...
Hope springs eternal.
Harshawn Singh and Nikolaus Heinen are thankful for the Science Technology Engineering Mathematic Summer Camp of the Manteca Unified School District.
It wasn't that anybody on the Manteca Unified Board of Education had anything against Roger Goatcher.
The news seemed good on the surface for Weston Ranch residents that are fed up with what they believe has been the turning of their ...
Jeff Zellner is living the American Dream.
Manteca's library has a whopping 91,000 books in circulation, but that's just the tip of the iceberg of what it provides.
There have been countless folks in Manteca who have touched my life and those of others in Manteca who are no longer with us.
Page 1 of 19
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6.13 out of 10 (what is this?)
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An education and training professional with over 20 years of corporate European/US Human Resource Development experience, coupled with a background in adult education. Professional memberships in UK and US HRD organizations. Current research interest - Technology tools for Education, Training and Development


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It only takes a minute to get started. Fill out our uncomplicated sign up form and login to the site.
This step is voluntary but will drastically raise your chances of people actually reading your profile and contacting you. Introduce yourself, your pasttimes and atach your nicest photo of yourself.
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Identified someone who appeals to you? Send a note to them or just a wink and see if anything happens. With a little luck you'll be on your way to locating your proper date.
And start dating


text:
Step Up TV Series Coming to YouTube
YouTube is stepping up to the original programming game!
The streaming service has given a straight-to-series order to Step Up, a drama based on the popular dance film franchise. READ MORE
YouTube is stepping up to the original programming game!
The streaming service has given a straight-to-series order to Step Up, a drama based on the popular dance film franchise. READ MORE
Freeform is making a big commitment to The Bachelor franchise.
ABC's sister cable net on Wednesday announced that it has ordered a spinoff of the dating juggernaut focused on former Bachelor Ben Higgins and fiancée Lauren Bushnell. READ MORE
Get ready to see more from Stevens and Lido on black-ish.
Peter Mackenzie, who plays Mr. Stevens aka Dre's boss at the aforementioned ad agency has been promoted to a series regular READ MORE
Nick Kroll (Kroll Show) and John Mulaney (Saturday Night Live) are headed back to high school by way of Netflix. READ MORE
Journalist David Carr's bestselling memoir The Night of the Gun is being developed by AMC and Sony Pictures Television as a six-part miniseries, written by Shawn Ryan (The Shield) and starring Emmy- and Golden Globe-nominated actor Bob Odenkirk (Better Call Saul). READ MORE
CBS has placed a 13-episode, straight-to-series order for Ransom, a dramatic thriller from X-Files/Man in the High Castle EP Frank Spotnitz starring Black Sails' Luke Roberts. READ MORE
Life just got really busy for Hunter King.
The Life in Pieces actress, who plays newlywed Clementine, has been promoted to series regular on the CBS comedy READ MORE
HBO has another hitman on its hands: The premium cabler has ordered to series Bill Hader's Barry READ MORE
Melissa McCarthy and Ben Falcone are bringing their nobody friends to TV Land. READ MORE
The hills are alive at Netflix with a new children's program starring prolific entertainer Julie Andrews.
The Sound of Music legend has teamed with the Jim Henson Company to launch READ MORE & WATCH VIDEO


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Auckland to host Volvo Ocean Race again
Relevant offers
Auckland will again play host to sailing's greatest adventure after winning selection as a host port in the Volvo Ocean Race for the next two series.
Auckland Council controlled organisation Auckland Tourism, Events and Economic Development (ATEED) announced today it had signed a deal for Auckland to play host in both 2014-2015 and 2017-2018.
The council will invest $5.5 million into the even and an economic study saying the races will generate $7.49m for the region.
Crews and support teams will be based in Auckland for 15 days of each race.
Auckland, a world renowned sailing capital, returned to the race route for the first time in 10 years in 2011-12 and staged a spectacular stopover at Auckland's Viaduct, with spectators packing the race village and the Waitemata Harbour.
Results from Google showed Volvo Ocean Race was the second most searched term in New Zealand in 2012.
The 2014-15 route will again see Auckland play host to the start of the main Southern Ocean leg, sending the teams on their way around Cape Horn and on to the leg finish in Itajaí, Brazil. The dates of the stopover will be revealed next month.
"Sailing into Auckland after a 10-year gap felt like the race was coming home," Volvo Ocean Race chief executive Knut Frostad said.
"Tens of thousands of passionate fans packed the Race Village each day, and the crowds for all the arrivals plus the in-port race and leg start were among the best we've ever had.
"Auckland people know sailing and know the race. As soon as we arrived it was clear that we'd been away from this stunning city too long and it's hugely satisfying to be able to say that we're coming back straight away this time. Having an agreement in place for the next two editions is just the icing on the cake."
This will be the ninth time the race has stopped at Auckland.
Auckland Mayor Len Brown said the win was exciting for Auckland.
"New Zealand's fabled sailing heritage, and the affinity Aucklanders have with the sea which surrounds us, makes this city the perfect Volvo Ocean Race destination.
- Auckland Now
Sponsored links
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Kart Racer (2003)
Plot Summary
14-year-old Watts Davies is estranged from his dad, a former International Karting Federation (IKF) champion. Watts's resolve to race in the upcoming IKF Regional Championships rekindles their relationship as they pursue the dream together.- Written by Anonymous
Be the first to contribute! Just click the "Edit page" button at the bottom of the page or learn more in the Synopsis submission guide.


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Director {DI}
Kellner Capital LLC
Direct Phone: (212) ***-****
Email: b***@***.com
900 Third Avenue Suite 1000
New York, New York 10022
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Kellner Capital is one of Wall Street's most seasoned hedge fund managers with more than a 30 year continuous operating history. Originally founded as a merger arbitrage investment partnership, today the Firm offers a variety of alternative investment str... more
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Colleen Gravley
Fore!Kids Foundation
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Florida Gulf Coast University
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text:
Illinois legislators reach deal on state's $100 billion pension problem
M. Spencer Green, File, Associated Press
CHICAGO — After more than five months of work, Illinois' legislative leaders announced Wednesday they've reached a deal to help solve the state's $100 billion pension problem, considered the nation's worst.
Leaders announced the agreement, but no details, following a Wednesday meeting.
House Speaker Michael Madigan's staff is putting together an "explanatory memo" for lawmakers and will send details of the proposed legislation to them Friday, said Madigan spokesman Steve Brown.
The Illinois House and Senate will consider the measure in a special session, which has been set for Dec. 3.
Lawmakers have faced considerable pressure to act on pension reform following years of unheeded calls for them to pass a savings plan. Years of skipping or shorting payments have resulted in multiple bond rating downgrades, with key funds being diverted from schools and social services.
The committee tasked with finding a solution was formed in June when lawmakers reached an impasse on competing plans. Gov. Pat Quinn later halted lawmakers' pay until the issue was resolved— a move later found to be unconstitutional by the courts.
The four leaders of the House and Senate have been working with a proposal developed over the summer and autumn by the 10-member committee of lawmakers. The committee plan would have saved the state $138 billion over 30 years.
Despite no knowledge of the details, the state's major employee unions said Wednesday they were opposed to the deal, saying they were left out of negotiations and that they believe elements are unconstitutional.
"We have tried for three years now to work with legislative leaders and the governor to develop pension reform ... that is fair to workers and retirees," American Federation of State, County and Municipal Employees union spokesman Anders Lindall said Wednesday.
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text:
As 4G network deployments grow, nearly a quarter of device subscription revenues will come from suites of operator-branded premium services, according to ABI Research's new study "4G Mobile Consumer Services." Total 4G mobile consumer service revenue — including mobile Internet services — will grow rapidly, exceeding $70 billion worldwide in 2014.
"Operators of 4G networks will refuse to be marginalized as 'dumb data pipe' service providers," said ABI Research practice director Philip Solis. "Instead, they will offer suites of 'smart services' — some internally developed, others via partnerships with third-party suppliers — that will be provided over 'smart networks' enabled with all-IP technologies, IMS infrastructure and cloud-based storage."
Expect 4G services optimized to enable bouquets of mobile devices — smartphones, netbooks and PNDs — Solis said. Operators will be offering pooled device subscriptions where one user subscription activates many devices. Because networks are data-only, Internet access service will be the "killer 4G service."
However, a suite of premium services can collectively drive significant consumer adoption, revenues and profits. The report specifically cites location services, such as turn-by-turn directions and points of interest; multimedia services, such as VoD and P2P video sharing; media broadcast services, such as pay-per-view TV and digital radio; and gaming services, such as multiplayer and augmented reality games.
These Web 3.0 services will be integrated with Web 2.0 features like personalization, community, interactivity, presence and localization, and delivered simultaneously, seamlessly and transparently to three screens — PCs, TVs and mobile devices — over the Internet, cable networks and wireless networks.
"Operators will take advantage of this market opportunity by breaking down their walls and building open ecosystems," Solis said. "They will partner with third-party service providers from whom they can license and rebrand services; they'll work with network and handset OEMs to influence infrastructure and device specs; and they'll join ecosystem development organizations, such as Alcatel-Lucent's ng Connect program."
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Yorgos Lanthimos and Richard Ayoade Direct New Radiohead Vignettes
DP Natasha Braier Takes 'The Neon Demon' to the Extreme


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Property for sale near Craiglea, Stirling FK9
Property type
More options
- Pre-owned / New homes No preference
- Ownership type No preference
- Retirement No preference
- Buyer incentives No preference
£345,000 Offers over
5 bed semi-detached house for saleCausewayhead Road, Stirling, Stirlingshire FK9
Virtual tour. An outstanding traditional sandstone semi-detached villa which has been modernised, upgraded and extended by the current owners to an excellent standard and enjoys an enviable setting within a highly desirable locale. (contd...)
Note: Distances are straight line measurements
- Stirling (1.3 miles)
- Bridge of Allan (1.8 miles)
£105,000 Offers over
2 bed flat for saleAbbey Craig Court, Stirling FK9
Centrally located close to city centre, this well appointed two bedroom ground floor flat with en suite forms part of established modern development with private parking.
Note: Distances are straight line measurements
- Stirling (1.3 miles)
- Bridge of Allan (2.1 miles)
Latest Craiglea, Stirling FK9 property for sale
Zoopla is one of the UK's leading property portals, helping you to find property for sale and to rent and make smarter decisions when buying and renting homes in the UK. Discover information on homes in Craiglea, Stirling FK9 by researching Craiglea, Stirling FK9 property values, Craiglea, Stirling FK9 house prices paid, our Craiglea, Stirling FK9 property market overview and find Craiglea, Stirling FK9 agents.