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advertising jobs near Jackson Heights, NY
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Advertising Agency Program Manager, North America
Veeva Systems - New York, NY
Three years or more experience in account, digital strategy, technology, or project management at a healthcare advertising/marketing agency....
Sr. Paid Search Specialist
True North Inc. - New York, NY
Advertising agency experience:. Digital advertising and media agency is looking for a technically proficient paid search specialist to support a growing media...
Partner-level employment litigator sought for NY area practice
Superior Legal Recruiting - New York, NY
The right attorney will have extensive experience with advertising, intake, initial demand and settlement, and litigation, and will have been in a senior role...
YBN Sales Director, NYC
Yodle - New York, NY
Advertising sales experience. Since 2005, Yodle has grown to 1,400+ employees, and we're not slowing down....
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Tremor Video - New York, NY
Experience with programmatic advertising is highly preferred. Awareness of online video advertising and programmatic platforms....
Business Development - Staffing Manager
Onward Search Inc. - New York, NY
Is the world of digital marketing and creative technology exciting to you? Here's what your career path could look like if you excel in this role:....
Regional Sales Manager - Chicago/NY/LA/Las Vegas/Philadelphia
ZLiving Inc. - New York, NY
An effective communicator and focused on advertising sales brand activiations. Specialize in Network TV Ad Sales and Digital Ad Sales experience....
Sales Director Mobile Programmatic
AdtechNation - New York, NY
2+ years' experience selling digital advertising. A leading VideoMobile Advertising company, having won several MMA (Mobile Marketing Association) Awards, is...
Account Executive Digital Media Sales
AdTechNation - New York, NY
Media, Agency or Brand advertising experience great to have but not required. Digital Media Ad Sales:. Strong working knowledge of Microsoft Office and CRM...
Wax Specialist
Plainview European Wax Center - Plainview, NY
Hourly wage, commission and bonus program. To encourage loyal guests, we offer incentives (packages and memberships) and use local and national advertising to...


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Coolest Firefox About:Config Tricks
You may have installed countless add-on in Firefox to enhance your using experience, but if you want to get the most out of Firefox, you really have to hack your way into the about:config.
The about:config page contains most (if not, all) of Firefox configuration options. It is so far the most effective, and the most powerful way to tweak and enhance your Firefox performance. Here are 28 of the popular tweaks.
Accessing your about:config page
In your Firefox, type about:config in the address bar.
about-configYou will be shown a warning page. Click the "I'll be careful, I promise!" button to proceed.
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Review: Linux Mint 18 (Sarah)
If you were looking to jump the Ubuntu ship completely, then we recommend taking a look at our recent Review of Fedora 24. It's equally as good as Mint 18 and equally worthy of your consideration. Between Linux Mint 18 and Fedora 24, we reckon it's exciting times in the Linux world. With the exception and onset of the boring world of vanilla Ubuntu releases, Linux feels reinvigorated and fresh once again. Jump on board, because it can only get better from here.
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More petroleum storage facilities to be built in Sri Lanka
Fri, Nov 16, 2012, 10:48 pm SL Time, ColomboPage News Desk, Sri Lanka.
Nov 16, Colombo: Sri Lanka Ministry of Petroleum Industry has handed over a project proposal to the National Planning Department to build a system of petroleum storage tanks.
The new tanks are to be constructed in Kolonnawa and Muthurajawela facilities. Seven tanks are to be built in Kolonnawa while three will be constructed in Muthurajawela.
The Ministry has estimated a cost of Rs. five billion for the project. The construction of the tanks is to be completed in 2015.
Ceylon Petroleum Storage Terminals Limited manages the Kolonnawa and Muthirajawela petroleum storage facilities.
ColomboPage - Recent 10 Stories
Sri Lanka trade deficit widens by 20.2 percent in March 2016-- [29 minutes ago]
New Governor for Sri Lanka Central Bank after Finance Minister returns from overseas -- [1 hour and 6 minutes ago]
Sri Lankan parliamentarian Udaya Gammanpila released on bail-- [8 hours ago]
Tamil Nadu coastal police bust Sri Lankan refugee trafficking racket-- [8 hours ago]
Japan provides Rs. 2.4 billion grant aid for implementation of the Maritime Safety Capability Improvement Project-- [8 hours ago]
FCID instructed to make arrests over investigations into companies owned by MP Namal Rajapaksa-- [9 hours ago]
Sri Lanka's Colombo inflation rises to 6.0 percent in June 2016
Sri Lankan Prime Minister to attend the third South Asian Diaspora Convention in Singapore on July 18-19
External debt of Sri Lankan government stood at US$ 23.1 billion by the end of April 2016
Sri Lanka exports 78 tonnes of fish in a week after EU ban lifted


text:
English Literature in Contextby Paul Poplawski
Pub. Date: 11/30/2007
Publisher: Cambridge University Press
Supporting the study of English literature from the Middle Ages to the present, this book is designed as an introductory text AND a helpful reference tool for an entire English Literature degree. Its key mission is to help students understand the link between the historical context in which the literature developed, how this has influenced the literature of the period and how subsequent periods in literature have been influenced by those that precede them. The book is carefully structured for undergraduate use, with a rich range of illustrations and textboxes that enhance and summarise vital background material. The seven chronological chapters are written by a team of expert contributors who are also highly experienced teachers with a clear sense of the requirements of the undergraduate English curriculum. Each analyses a major historical period, surveying and documenting the cultural contexts that have shaped English literature, and focusing on key texts. In addition to the narrative survey, each chapter includes a detailed chronology, providing a quick-reference guide to the period; contextual readings of select literary texts; and annotated suggestions for further reading.
Product Details
- 9780521839921
- Publisher:
- Cambridge University Press
- Publication date:
- 11/30/2007
- Pages:
- 686
- Product dimensions:
- 6.85(w) x 9.72(h) x 1.77(d)
Table of ContentsList of illustrations; Notes on contributors; Preface; Acknowledgements; Preface Paul Poplawski; 1. Medieval English, 500-1500 Valerie Allen; 2. The Renaissance, 1485-1660 Andrew Hiscock; 3. The Restoration and eighteenth century, 1660-1780 Lee Morrissey; 4. The Romantic period, 1780-1832 Peter J. Kitson; 5. The Victorian age, 1837-1901 Maria Frawley; 6. The twentieth century, 1901-39 Paul Poplawski; 7. The twentieth century, 1939-2004 John Brannigan; Index.
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When might one take Tram in Paris ? Is there an advantage to taking a tram vs a bus, and where would I go to board a tram ? Would I swipe a Navigo Decouverte Pass or insert it somewhere? Is there a route map for trams?
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Trams in Paris
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Here is some information from RATP, the mass transit operator, on its new(ish) tramlines.
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The trams only follow a circular course around the outskirts of Paris near the périphérique. Unless you're on the outskirts you won't encounter them.
To learn how to get around Paris by métro/bus/RER/tram use www.ratp.fr. Here is an interactive map of the system:
Since parts of this website are only in French you can use www.transilien.com or www.vianavigo.com. A good website to learn how to use public transport in Paris is www.parisbytrain.com.
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Most of Europe's new wave of trams are essentially overground mini-metros, connecting bits of suburbia with each other. Usually (as in Paris, London or Florence) they link places bus don't link, or link only by a series of life-destroying connections.
Though there are tram networks in many cities (like Milan) that are essentially unchanged from the 19th century and operate like spokes from the heart of the city, programmes to reintroduce such systems elsewhere aren't looking too promising. Much as tramophiles like to pretend otherwise, successful new tram plans aren't about bringing them back into the centre (there usually just isn't room): they're about linking outlying areas together better than other alternatives (including private cars) can.
As a general rule the places they link are those visitors just won't be interested in. Manchester is the one exception: Europe's best black puddings are a few minutes' walk from the new Bury tram stop, the famous Eccles Cake originated close to Eccles' new tram link and trams are the best way to get to the Etihad Stadium.
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*In an emotional interview with television station WWOR, Cissy Houston shared some intimate memories and moments about her daughter.
It was her first interview since Whitney Houston's death.
Although she's proud of her girl, the mourning mother said her daughter was in a good place at her death.
"I'm very proud of my daughter; she accomplished a whole lot in the short time that she was here," Cissy said, "She's not broke. She's not anything, none of that crap."
"The media are awful. People have come from here and there, [and they] don't know what they're talking about. People I haven't seen in 20 years…. Here they come, [they] think they know everything, but that's not true," she said. "They really chopped on her, chopped on her…kept, kept, kept."
Overall, however, the matriarch of the family has no regrets about how she raised Whitney and that she made her proud, despite the dark days.
"I don't blame myself, I know I did the best I could for everything."
By the way, Cissy has returned to her gospel roots, and is back to singing. The Grammy Award winning songstress is ready to debut her newest song, "Walk On By Faith," and she shared some of the song's very personal lyrics during her interview.
"You can't see in the future, you can't see through dark clouds. You can't see all the pitfalls, so walk on by faith each day."
Watch the interview:
CISSY HOUSTON'S FIRST INTERVIEW SINCE WHITNEY'S DEATH: My9TV.com
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The International Wine Investment Fund (IWIF) has upped its stake in the French wine producer Group Laroche and says it is prepared to take its investment furhter under the right conditions. The IWIF, controlled by Berren Asset Management, has almost doubled its stake from 4.1% to 8.1%, an investment worth A$1.1m. The Fund has already invested some $1.3m in Laroche.
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N.J. Gov. Chris Christie is upset about the attack on LGBT nightclub Pulse in Orlando. In response, he wants the U.S. to respond with military action overseas.
"You've got to get over there and make them pay where they live," Christie said yesterday on WFAN's Boomer & Carton Show (yes, a sports talk show). He was filling in for Boomer Esiason. "It is unacceptable to allow this type of stuff in our country and for us to not fight back. We've got to fight back."
Christie is a top adviser to presumptive Republican presidential nominee Donald Trump and the head of Trump's White House transition team if he wins. Earlier in the day at a public event, Christie said people need "to get serious" at "every level in government and in the private sector" about the threat of "radical Islam." Read more »


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Early applied demonstrations of new analytical methods with clear societal impact
Impact Factor 1.915 48 Issues per Year Indexed in Web of Science
Effect of oxidative treatment of carbon black on electrochemical activity of
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text: The Ice Cube Conspiracy By The Message Is Ice Cube a clone? Published June 13, 2007 9.2k views More Info » Full Credits The Message Uploader Stats & Data 249 Funny Votes 122 Die Votes 9,199 Views Published: June 13, 2007 Categories: Sketch Keywords: Ice Cube nwa yet rap conspiracy movie hip hoplaugh Up Next: Summer of Fear! AUTOPLAY Up Next In: 10 Summer of Fear! Black Love is Dead American Apparel's Next Top Model The Race Card Meets Its match Follow Facebook Twitter Tumblr Instagram Browse Related Comments 20+ more View All ▶ The Message Vaguely Related Videos Vaguely Related Pics & Words Advertisement Tags: Ice Cube nwa yet rap conspiracy Advertisement


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Learn how to make authentic Indian cuisine in this hands-on workshop with Nimita of Nimita's Cuisine, followed with a lovely multi-dish dinner together. It will be fun to cook together and enjoy blending the aromatic and flavorful spices!
Nimita has been teaching since 1994 in the Canary Islands, Spain and here in Santa Barbara since 1996. She uses the freshest vegetarian ingredients, blending spices to create nourishing Ayurvedic meals.
This special workshop is a fund-raiser for our Santa Barbara Culinary Arts group which supports local culinary students with our Endowment of the Scholarship in Honor of Julia Child at SBCC. The cost is only $50 for SBCA members, $60 for non-members for the workshop, delicious meal and leftovers! Please RSVP to secure your spot: [masked].
About the class:
"The class lasts approximately three hours in order to cover a full menu. Recipe booklets will be provided so that the participants can have their full hands on experience as they learn to master the use of spices. All vegetables are locally grown. The ingredients used are of the highest quality since this will enhance the meal to be healthy, nourishing, and healing at the same time. After the meal is prepared, we will sit together to enjoy our makings and company together. I encourage you to bring Tupperware for leftovers to share with your family and friends since we tend to make extra."
"Cooking class unlike anything you've done before. Instead of being recipe driven, it's about how to cook: learning what spices to use, how to use them, and how to honor the food you're preparing as well as the people eating it. Can't wait to go again!" --Rose Pizzorno (SBCA member)
Please join us! To secure your spot, please RSVP to Karyn: [masked] or call 895 • 6750
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Jim Breuer (Saturday Night Live)
Aladdin Theater, at SE Powell Blvd 3017 Southeast Milwaukie Avenue Portland, OR 97202
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Comedian Jim Breuer came to fame on Saturday Night Live, where he was known for characters like Goat Boy and impressions of Joe Pesci, among others. Along with a successful film career -- including a co-starring role with Dave Chappelle in stoner cult-comedy Half Baked -- his high-energy stand-up has been showcased on The Late Late Show with Craig Ferguson, Jimmy Kimmel Live, Late Night with Conan O'Brien, as well as on NBC's The Marriage Ref and on his hour-long Comedy Central special, Let's Clear the Air. He's a frequent guest on radio's The Opie & Anthony Show, and has his own weekly show on Sirius Satellite Radio. Comedy Central has named him one of the 100 greatest stand-ups of all time, and he continues to tour the world today, cracking up audiences with his skewed observational comedy.
There are currently no offers for Comedian Jim Breuer.
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Justin Willman (The Tonight Show): Magic and Comedy
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- $15.00 - $25.00
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Not just the host of Food Network's Cupcake Wars and Last Cake Standing, Justin Willman is also an… More
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I heard it was going to be hard to park, but we arrived about 45 minutes before the show and found street parking right across the street. Then just got a glass of wine inside and drank it at our seats, relaxed while waiting for the show.star this tip starred
There was beer, wine and some food there. There is a variety of places to eat within walking distance, too.star this tip starred
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About the Ticket Supplier: True West
True West is the premier live music promoter for shows in the Northwest.


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Army of Darkness Video Comparison
There are now seven versions of Raimi and Campbells epic, Army of Darkness on DVD and therefore a lot of people are interested in ...
If you wish to download the larger images then please click here. They are in JPEG form to reduce the size slightly, but with little compression (15% - Paint Shop Pro 7 JPEG) used. The file is a little over 1Mb in size. You will require WinRAR to uncompress it (you can download WinRAR from here).As you can see from the above, unfortunately the director's cut versions of the film are quite soft with a lot lighter colouring. Also, some of the print is cropped out for some reason. This would make the theatrical version the way forward with its superior transfer, however you then miss out on the extra footage and the alternative ending. This leaves one option: the R2 Anchor Bay 2 disc since both versions are included, its relatively cheap when compared to the R1 versions which is supposedly the same except the R2 has a DTS-ES 6.1 audio track on the theatrical version. Look for a full review in the next day or two.
Editorial by David Beamish
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|Publication number
|Publication type
|Application number
|PCT number
|Publication date
|Dec 20, 2011
|Filing date
|May 15, 2003
|Priority date
|May 16, 2002
|Fee status
|Also published as
|Publication number
|Christian Bergaud, Matthieu Guirardel, Pascal Belaubre, Benoît Belier, Jean-Bernard Pourciel
|Original Assignee
|Centre National De La Recherche Scientifique
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (11), Non-Patent Citations (2), Referenced by (2), Classifications (24), Legal Events (2)
|External Links: USPTO, USPTO Assignment, Espacenet
This application is a national stage application filed under 35 U.S.C. 371 of International Application No. PCT/FR03/01481, filed May 15, 2003, which claims priority from French Application No. FR0206016, filed May 16, 2002.
The subject of the present invention is a device for the actively controlled, localized deposition of at least one biological solution in the form of microdrops.
In the pharmaceutical industry, the capital invested in research for developing new medicines occupies a considerable portion of an enterprise's budget.
New assay methods are needed to reduce the cost of such research.
The arrival of microchips in the biomedical sector has revolutionized the fields of medicinal product development and of bioassay.
The advantages of these microchips are the following:
they allow new, more sensitive detection methods to be developed;
However, the tools that are currently operational for distributing small volumes of biological material in solution allow the deposition, on glass slides or on membranes, of drops with a diameter of the order of a hundred microns (which corresponds to a drop volume of the order of a nanoliter). These systems rely:
Other deposition techniques that have formed the subject of laboratory studies are known, these making it possible to achieve smaller volumes than those obtained with the abovementioned operational tools.
One of these techniques is dip-pen lithography, which is a technique derived from atomic force microscopy and makes it possible to form features on a surface using a molecular transport diffusion effect at the water meniscus that forms between the tip of an atomic force microscope and the surface on which the deposition takes place. The operating principle relies on the difference in hydrophilicity or wettability properties between the tip and the surface. The surface must in fact be more hydrophilic than the tip in order to cause molecular diffusion from the tip toward the surface. The resolution obtained may be less than 1 micron and it is also possible to envisage the deposition of different biological molecules, but this means changing the tip (which will have been immersed beforehand in the solution to be deposited) for each solution. This deposition technique is therefore extremely time consuming if it is desired to carry out several tens of different depositions. Moreover, changing the tip of the microscope does not make it possible to maintain the alignment precision between two changes. Finally, this approach can be implemented only under high humidity conditions in order for the water meniscus to form.
This technique is described in particular in the following articles:
Other Microsystems have also been proposed for carrying out depositions for the fabrication of biochips. These apply in general to microfluid structures, for example that described in the following article:
These are micromachined silicon structures having microfabricated channels, and their use is altogether comparable to that of an ink jet system. These "closed" structures, in the form of tubes, are very difficult to clean, which represents an obstacle to the same device being used to deposit droplets of different liquids.
International patent application WO 02/00348 illustrates a deposition system that allows microdroplets with a volume of between 10 picoliters and 200 nanoliters to be deposited. Such a system consists of at least one lever, made of silica or quartz, equipped with a capillary channel and with a reservoir. The liquid is picked up and deposited purely passively, by capillary effect and by the difference in wettability between the device and the deposition surface.
Micropipettes allowing contactless deposition, by means of a field effect, are described in particular in the following documents:
These devices exploit the electrospray effect in order to deposit in a controlled manner, by means of an adjustable electric field, very small amounts of organic molecules. However, the electrospray method consists in applying an electric field high enough to ionize and atomize the liquid to be deposited. The droplets thus produced have submicron dimensions and evaporate before they reach the deposition surface; in this way, thin films are produced. This is therefore a different problem from that facing the present invention, that is to say the deposition of droplets with a volume of the order of 1 picoliter or 1 femtoliter. In addition, the electrospray devices consist of micropipettes containing a needle-shaped electrode; they cannot therefore be effectively washed and have to be replaced each time the liquid is changed.
Studies on surface wetting under the effect of an electric field and the displacement of a liquid by actively controlling the wettability of a surface have been published in the following articles:
These articles describe the physical principles of electrowetting and dielectrophoresis, and also their application for handling droplets of liquids such as water. Although these effects have been known for several decades, they have never been applied to the deposition of liquid droplets.
In conclusion, no deposition system has yet been proposed that allows microdrops with a diameter of less than 10 microns, that is to say with a volume of less than 1 picoliter (p1), to be deposited in an actively controlled and precise (relative to a reference) manner.
A fortiori, no known deposition system allows such drops to be deposited in a precise and actively controlled manner on microstructures of the bridge, beam or membrane type.
The present invention makes it possible to achieve these objectives by the use, as deposition system, of one or more silicon microlevers having at least one electrode for handling the liquid to be deposited by electrostatic effects.
One subject of the invention is a deposition device for precise localized and actively controlled deposition of microdrops, in particular with a diameter of less than 10 microns, and more particularly with a diameter of the order of 1 micron.
Another subject of the invention is a deposition device for precise localized and actively controlled deposition of microdrops on microstructures such as bridges, beams or membranes.
Another subject of the invention is a deposition device for depositing different biological molecules.
Another subject of the invention is a deposition device for depositing microdrops without any contact with the structure or the microstructure on which the deposition takes place.
Another subject of the invention is a deposition device for depositing microdrops by contact with a structure or microstructure, under conditions that maintain the integrity of the structure or microstructure.
At least one of the aforementioned objectives is achieved by means of a device for depositing biological solutions, comprising at least one flat silicon lever having a central body and an end region that forms a tip in which a slit or groove is provided, characterized in that it has at least one metal track that is provided on one face of the central body and that runs at least partly alongside a said slit or groove.
Advantageously, said slit or groove extends from said tip as far as a reservoir provided in the central body.
Advantageously, said metal track or tracks run at least partly alongside said reservoir.
According to one embodiment of the device, the reservoir is a non-emergent cavity provided in one main face of the central body.
According to another embodiment, the reservoir consists of an emergent opening provided between two opposed main faces of the central body.
A said slit or groove and/or a said reservoir and/or a said metal track is/are optionally coated with SiO2.
Advantageously, the lever has at least one hydrophobic region made of silicon or else made of silicon oxide coated with a hydrophobic silane.
Advantageously, the device has at least one implanted piezoresistor.
Advantageously, the or each lever has at least one integrated actuator for controlling its bending.
According to a preferred embodiment, said actuator comprises a piezoelectric layer deposited on a surface of said lever.
According to another preferred embodiment, said actuator comprises a bimetallic strip and a heating resistor that is deposited on a surface of said lever.
The invention also relates to a process for fabricating a device as defined above, characterized in that it involves:
The process may be characterized in that b) also includes:
The process may be characterized in that c) comprises chemical or ion etching down to the buried insulating layer in order to define, in addition to the outline of the levers, a slit and/or an emergent opening constituting a reservoir for at least one lever.
The process may be characterized in that c) comprises first chemical or ion etching of the substrate, this operation being stopped before the buried insulating layer in order to define at least one groove and/or a non-emergent cavity forming a reservoir, for at least one lever, and second chemical or ion etching of the substrate down to the buried insulating layer in order to define at least the outline of the levers.
The first chemical or ion etching may be carried out in such a way that the outline of the levers is defined over part of their thickness.
Advantageously, before a), a step of implanting at least one piezoresistor is provided.
Advantageously, the process also includes a step of depositing an integrated actuator.
According to a preferred embodiment, said step of depositing an integrated actuator comprises the deposition of a piezoelectric film of PbZrO3/PbTiO3 by sputtering.
Advantageously, said piezoelectric film is isolated from the liquid by a layer of a material chosen from the following: silicon oxide, "Teflon" PTFE, a polymer.
According to another preferred embodiment, said step of depositing an integrated actuator comprises the low-pressure chemical vapor deposition (LPCVD) of an Si3N4 layer followed by deposition, by evaporation, of a Cr layer and of an Au layer in order to produce a heating resistor, thus forming a bimetallic strip.
The invention also relates to a method of sampling at least one biological solution using a device as defined above, characterized in that the sampling and the retention of said biological solution are assisted by an electric field effect by applying a potential difference between said metal tracks.
If a device having a piezoresistor is used, advantageously a measurement of the variation in the electrical resistance of said piezoresistor is made after the sampling, in order to determine the amount of biological solution taken.
The invention also relates to a method of depositing at least one biological solution using a device as defined above, characterized in that the deposition of said biological solution is assisted by an electric field effect by applying a potential difference between said metal tracks, which are maintained at the same potential, and a deposition surface having at least one conducting layer.
If a device having a piezoresistor is used, advantageously a measurement of the variation in the electrical resistance of said piezoresistor is made after the deposition, in order to determine the amount of biological solution deposited.
The invention also relates to a method of depositing at least one biological solution using a row of devices as defined above, each having a piezoresistor and an integrated actuator, characterized in that the contact force of each lever with the deposition surface is determined by measuring the variation in the electrical resistance of each implanted piezoresistor that is actively controlled by each integrated actuator.
Other features and advantages of the invention will become more clearly apparent on reading the following description in conjunction with the appended drawings in which:
As may be seen in
The geometrical dimensions of the levers may be the following:
Lever length:
1 to 2 mm
100 to 300 μm, for example 210 μm
1 to 20 μm (depending on the thickness
of the initial SOI substrate)
Inter-lever gap:
450 μm (for example)
Channel length:
200 to 400 μm, for example 250 μm
(when a reservoir is drawn); 200 to
1000 μm, for example 550 μm (with
no reservoir)
Channel width:
2 to 20 μm, for example 5 μm
Reservoir length:
200 to 600 μm, for example 250 μm
Reservoir width:
50 to 150 μm, for example 80 μm
Width of the conducting tracks:
1 to 40 μm, for example 20 μm.
The channel may be a groove 4 provided over part of the thickness of the lever starting from a surface 11, or a through-slit 5 that extends between the faces 11 and 12. The channel may communicate with a non-emergent reservoir consisting of a cavity 6 provided in a main face 11 of the central body 1 of the lever, or else with an emergent reservoir 7 consisting of an opening 7 provided between the main faces 11 and 12 of the central body 1.
The metal tracks 8 and/or 9 run alongside the reservoir 6 or 7 (
An actuator may be integrated on the rear face of the levers, this consisting of a piezoelectric layer 38 (
A piezoresistor 31 may also be integrated on the rear face of the levers (
Both the piezoresistor 31 and the actuator 33-35-37 or 38 are isolated from the liquid by a passivation layer 32.
The device according to the invention allows in particular:
Deposition on microstructures, mentioned in item (g), constitutes a major advantage of the invention, since such devices can be used as integrated biomolecule detectors. In this regard, see the articles:
With regard to the actuator mentioned at point (i), this allows only part of the levers constituting a row, as illustrated in
The process for fabricating deposition levers is based on the collective fabrication techniques used in microelectronics. A series of technological steps is carried out on an SOI (Silicon On Insulator) substrate.
The first part of the process comprises a succession of thin-film formation steps (
The first step (
During the step shown in
The last step of the thin-film part is a second localized deposition 26 of silicon oxide (
To start the micromachining, front face photolithography in the silicon layer 27 allows the outlines of the levers to be defined. A first plasma etching operation (reactive ion etching or RIE) is then carried out on the silicon oxide, and then a second plasma etching operation is carried out on the single-crystal silicon (
Lastly, a final photolithography operation, starting from the rear face 28 of the wafer, and then a deep reactive ion etching (DRIE) operation on the silicon layer 29 are carried out in order to free the levers (
When etching the outlines of the levers, several options are possible depending on the desired outline. For levers with an emergent channel (a slit 5 passing through the entire thickness of the lever) with or without a reservoir, a single step (as shown in
However, to etch non-emergent structures (groove 4 or cavity 6), two photolithographic steps followed by etching have to be carried out in succession. The first, which defines the channel 4 and/or the reservoir 6, must be stopped before the intermediate oxide layer of the SOI substrate is reached. This step must therefore be supplemented with photolithography and etching of just the external outlines of the levers down to the intermediate oxide layer of the SOI substrate.
The optional implantation of at least one piezoresistor, placed for example longitudinally in the body 1 of the lever, may be carried out before the step shown in
One or more piezoresistors implanted on at least some of the levers make it possible for there to be one or more strain gauges, the resistance variation of which is used to detect, in particular, when the lever comes into contact with a surface. This makes it possible in particular to ensure control of the coplanarity of the levers during collective deposition.
Optionally, a piezoelectric film 30, for example consisting of a mixture of PbZrO3 and PbTiO3 in a 54/46 ratio may be deposited by sputtering, as described in:
The deposition may be carried out, for example, on the rear face of the lever, as illustrated by
Alternatively, the actuator may consist of a bimetallic strip.
The metal tracks constitute the core of the invention, as they make it possible to control the rise of the liquid into the slit or groove, when filling the device, and its descent during deposition, by field effect.
A first technique, called dielectrophoresis and proposed by Jones et al. (see the document mentioned above), consists in using an AC electric field to confine a polarizable liquid (for example water) in areas of high electric field (the use of a DC field is possible, but it may cause undesirable effects, such as electrolysis of the liquid or it may damage biomolecules). Since this field is created between two coplanar insulated electrodes, the liquid is literally "pressed" against the electrodes. A very similar effect, the physical origin of which is different, occurs in the case of conducting liquids. Moreover, it is important to consider that a liquid may be "conducting" or "dielectric" depending on the frequency of the electric field that is applied thereto. If, within a given frequency range, the liquid constitutes a dielectric, the electrodes need not be coated with an insulator. Another technique, known as electrowetting, allows the wettability properties of a surface (contact angle between the surface and the liquid) to be modified by applying a potential difference between said surface and the liquid, and thus the capillary effects may be controlled. If a potential difference of a few volts to 10 V is applied between the electrodes and a conducting surface, the field effect may cause contactless deposition. A higher potential difference (above 1 kV) may result in electrospraying.
Several surface treatments may be carried out on the levers in order to make them hydrophilic or hydrophobic, so as to optimize the behavior of the liquid deposited on the surface.
Firstly, it is possible to vary the materials derived from silicon, knowing their properties: silicon oxide is thus used as hydrophilic compound, and single-crystal silicon is used as hydrophobic material.
However, since silicon has a natural tendency to undergo surface oxidation (presence of a nascent oxide), it may be necessary to carry out a chemical surface treatment. Such a treatment consists for example in attaching a hydrophobic silane, for example a silane having a methyl or fluorine-containing group as end group, which silane is deposited on the silicon oxide. This compound is deposited on silicon oxide in the form of self-assembled monolayers and has the advantage of being highly hydrophobic.
Alternatively, it is conceivable to use techniques in which remanent charges are created in the oxide, by implantation or irradiation (for example using X-rays), in order to enhance the wettability or hydrophilicity properties of the passivation layer (for example, a cold oxide layer).
In a preferred embodiment of the present invention, the surface of the device is made highly hydrophobic and liquid is picked up by means of the abovementioned dielectrophoresis and electrowetting effects. This makes it easier to clean the device and makes it possible to deposit several different liquids without contamination.
A three-axis (X, Y, Z) microrobot allows the microlevers according to the invention to be used for the filling and deposition phases.
The pick-up phase entails dipping the microstructures into a reservoir containing the solution to be deposited and filling the microchannels by field effect, optionally assisted by capillary effect.
For the deposition phase, the microrobot is used to position the microstructures very precisely with respect to a surface intended to receive the deposit. Deposition then takes place by direct contact with the surface or by contactless field effect. The spray deposition technique can also be considered if the field applied is high enough to cause spray generation and atomization of the biomolecules.
The robot is, for example, a commercially available three-axis (X, Y, Z) robot with a 50 nanometer step, readily compatible with diameters of around 10 to 20 microns of the deposits to be produced. This precision allows fine control of the lever/deposition surface contact, thus giving better volume uniformity of the spots produced. Further improvement of the contact control is achieved by using an actuator, for example a piezoelectric or thermomechanical actuator, integrated into the microstructure. In addition, in the case of a row of levers, the integrated actuators allow the contact of each device with the surface to be individually controlled.
The integrated piezoresistors allow servocontrol of the robot and said actuators.
Displacement along each axis is provided by a stepper motor. Each motor, supplied with AC current, is associated with a linear position sensor, allowing closed-loop position control.
The angle of incidence, that is to say the angle of contact between the lever and the surface on which the deposition is carried out, has an appreciable influence on the size of the drops deposited. The most satisfactory results are obtained with an angle close to 60°. It should be noted that, during the contacting phase, this angle varies from 60° to 45° when lowering the lever after contact by 50 microns (i.e. the value of the distance through which the lever is lowered after contact, which hereafter will be termed the "depth of contact"). Thus, the volume of liquid deposited is varied by applying a higher or lower bearing force.
The angle can be varied by means of a movable part fixed to the Z axis and rotating with respect to the Y axis. It is possible to control this angle directly by means of microcontrollers connected to the drive system.
The deposition step may be carried out in the following. manner, as illustrated by
The first step (
We therefore have two options: either the robot moves the levers against the surface and the deposition takes place by contact (
In the case of contact deposition, the volume deposited depends on the depth, the contact angle and the contact time. The field effect may also be used to control the volume of the deposit—reducing the electric field between the conducting tracks increases the amount of liquid deposited, and vice versa. If a row of levers is used, deposition by each lever is individually controlled thanks to the integrated actuators, which act on the characteristics of the contact, and the electrodes.
In the case of contactless deposition, a potential difference of a few volts up to 10 V is applied between the metal tracks and the deposition surface, which has to be conducting or to have a conductive coating. Thus, the resulting field effect (dielectrophoresis) sucks up the liquid. A higher potential difference (above 1 kV) may result in an electrospray.
This procedure is repeated for each set of spots to be deposited, according to a program set up by the user, until the number of spots that can be produced without refilling have been reached. If this situation occurs, the robot interrupts the deposition task and resumes that of picking up liquid.
|Cited Patent
|Filing date
|Publication date
|Sep 12, 1975
|Sep 19, 1978
|Amp Incorporated
|Bimetal actuator
|Apr 4, 1990
|Feb 4, 1992
|Westonbridge International Limited
|Micropump having a constant output
|Nov 13, 1998
|Aug 15, 2000
|Telechem International Inc.
|Microarray printing device including printing pins with flat tips and exterior channel and method of manufacture
|Jun 10, 1999
|Jan 30, 2001
|Gesim Gesellschaft Fur Silizium-Mikrosysteme Mbh
|Microejector pump
|Feb 5, 1998
|Aug 28, 2001
|Hahn-Schickard-Gesellschaft Fur Angewandte Forschung
|Microdosing device and method for operating same
|Mar 26, 2001
|Mar 11, 2003
|Tecan Trading Ag
|Mar 16, 2001
|Jan 10, 2002
|O'connor Stephen D.
|Electrostatic systems and methods for dispensing liquids
|Jul 20, 1999
|Feb 1, 2001
|Max Planck Gesellschaft
|Nadel und Verfahren zum Transfer von Liquiden sowie Verfahren zum Herstellen der Nadel
|Nov 1, 1999
|May 11, 2000
|Biorobotics Ltd.
|Liquid transfer system
|Jul 12, 2000
|Jan 18, 2001
|Biorobotics Limited
|Liquid transfer pin
|Jun 26, 2001
|Jan 3, 2002
|Renne-Industries B.V.
|Device for metered collection and dispensing of liquids, method for manufacturing such a device and methods for collecting and dispensing liquids
|International Search Report for PCT/FR03/01481, filed May 15, 2003.
|Sen et al. A Novel Pump for MEMS Applications. Journal of Fluids Engineering, vol. 118, 1996.
|Citing Patent
|Filing date
|Publication date
|Oct 22, 2012
|Mar 22, 2016
|Cornell University
|Multiplexed electrospray deposition apparatus
|Feb 21, 2013
|Cornell Univeristy
|Multiplexed Electrospray Deposition Apparatus
|U.S. Classification
|417/413.2, 427/2.1, 239/696, 417/413.3
|International Classification
|Cooperative Classification
|European Classification
|Nov 29, 2005
Owner name: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, FRAN
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:BERGAUD, CHRISTIAN;GUIRARDEL, MATTHIEU;BELAUBRE, PASCAL;AND OTHERS;REEL/FRAME:017080/0044;SIGNING DATES FROM 20050210 TO 20050310
Owner name: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, FRAN
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:BERGAUD, CHRISTIAN;GUIRARDEL, MATTHIEU;BELAUBRE, PASCAL;AND OTHERS;SIGNING DATES FROM 20050210 TO 20050310;REEL/FRAME:017080/0044
|May 29, 2015
|Fee payment
Year of fee payment: 4


text:
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I'd like to put shoes in perspective for the masses. In particular, the universal obsession women have with shoes. As one of those women, I feel that I have the... More
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Today we're going to talk about music. Music is a complex and sometimes very unappealing organization of noise that is written by a composer who will likely be poor... More
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This week is a special week. Not because school looms on the horizon, not because of the panicked scramble for on sale pencils and notebooks.
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WASHINGTON (AP) — Texas Rep. Pete Sessions says he's dropping bid for House majority leader.
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The University of South Australia's Aviation Academy will run a seven-day-a-week aerial shark patrol service this summer, starting on Saturday (December 1) through to the end of March.
Emergency Services Minister Jennifer Rankine has announced UniSA will provide the service, which this year includes an extra 170 hours of shark patrols along the metropolitan coast line.
Previously UniSA ran the week-day patrols but will now also provide the patrols on weekends and public holidays after winning the SA Government contract for the next three summer seasons.
UniSA's daily patrols will run from North Haven to Rapid Bay from 11am to 8pm, while in the school holidays patrols will be extended to include daily flights along the south coast between Victor Harbor and the Murray Mouth.
UniSA Head of Aviation Neil Hyland says the Aviation Academy is delighted to be deployed for more hours over the summer, giving young pilots valuable flying experience and providing an important community service.
"Shark patrol provides an invaluable opportunity for graduate pilots and crew to gain real operational experience," Mr Hyland said.
"The shark patrol is an example of the diverse career opportunities available for graduate pilots, who are in demand in areas like fish spotting, 'fly-in-fly-out' for mining, instructing, charter, and of course regional, national and international airlines.
"Flying the shark patrol provides them with an insight into the wide range of career prospects and the professional responsibilities of air crew."
UniSA uses fixed-wing aircraft for its patrols. When a shark is sighted, the aircraft will circle the shark, at the same time sounding a loud siren.
Shark patrol hours last summer reached 734. This summer there will be more than 900 hours.
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Marco Polo Plaza Cebu, Visayan Islands
- Free WiFi
Main amenities
- 329 guestrooms
- 3 restaurants
- Full-service spa
- Outdoor pool
- Breakfast available
- Internet in the lobby
- Health club
- Business center
- Airport shuttle
- Air conditioning
- Front desk safe
- ATM/banking services
What's around
- Heart of Cebu
- Gaisano Country Mall (1.5 mi / 2.5 km)
- Parkmall (1.5 mi / 2.5 km)
- Cebu IT Park (1.6 mi / 2.6 km)
- Banilad Towncenter (1.6 mi / 2.6 km)
- Cebu International Convention Center (1.9 mi / 3 km)
Marco Polo Plaza Cebu, Visayan Islands
Hotel Info: 866-539-8117from $74
- Superior Room, 2 Double Beds
- Continental Club Room
- Grand Deluxe Room
- Deluxe Double Room
- Grand Deluxe Room - Single
- Continental Club Room - Single
- Deluxe Single Room, 1 Double Bed
Also popular with travelers from the USA
At a glance
Key facts
Hotel size
- This hotel has 329 rooms
- This hotel is arranged over 24 floors
- Check-in time starts at 2 PM
- Check-out time is Noon
- Express check-in/out
Required at check in
- Credit card or cash deposit required
- Government-issued photo ID required
Travelling with others
- Pets not allowed
- Free WiFi in public areas
- Free WiFi in rooms
- Airport shuttle *
- Area shuttle *
Nearby Four Star Visayan Islands hotels
In the hotel
- Breakfast daily (surcharge)
- 3 restaurants
- 24-hour room service
- Outdoor pool
- Children's pool
- Health club
- Full-service spa
- Spa treatment room(s)
- Bicycle rentals on site
- Hiking/biking trails nearby
- Mountain biking nearby
- Sauna
- Business center
- Conference space
- One meeting room
- Dry cleaning/laundry service
- Hair salon
- Wedding services
- Multilingual staff
- Porter/bellhop
- Number of buildings/towers - 1
- Year Built 1979
- Elevator/lift
- ATM/banking
- Safe-deposit box at front desk
- Designated smoking areas
- Accessible bathroom
- In-room accessibility
In the room
- Air conditioning
- Minibar
- Coffee/tea maker
- Bathrobes
- Slippers
- Hypo-allergenic bedding available
- Blackout drapes/curtains
- Premium bedding
- Separate sitting area
- Private bathroom
- Shower/tub combination
- Bidet
- Free toiletries
- Hair dryer
- Cable TV channels
- Desk
- Free WiFi
- Phone
- Refrigerator
- In-room safe
Top Visayan Islands hotels
Recently reviewed hotels in Visayan Islands
- Azia Suites and ResidencesFrom
"Nice stay!! Very clean ....awesome staff .. Loving the location "Jun 12, 2016
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"Stayed at the Golden Peak before and had a nice stay. This year our room was bigger. However, the internet wifi was very poor. The water in the shower was just barely warm. The…"Jun 10, 2016
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Nearby hotels
Special features
The onsite spa has massage/treatment rooms.
DiningCafe Marco - This restaurant serves breakfast, brunch, lunch, and dinner.
BLU Bar & Grill - Onsite restaurant. Guests can order drinks at the bar and enjoy alfresco dining (weather permitting). Open select days.
El Viento - This restaurant is located by the pool. Guests can order drinks at the bar and enjoy alfresco dining (weather permitting).
Onsite venue #4 - lobby lounge.
Marco Polo Plaza Cebu, Visayan Islands's small print
Also known as
- Marco Polo Cebu Plaza
- Marco Polo Hotel Cebu Plaza
- Marco Polo Plaza Cebu
- Marco Polo Plaza Cebu Island/Cebu City
- Marco Polo Plaza Hotel Cebu City
Construction is underway at a neighboring building and there may be noise disturbance from the construction work.
Optional extras
Breakfast is offered for an extra charge of PHP 757 per person (approximately)
Airport shuttle service is offered for an extra charge
High-speed Internet access (wired) is available in guestrooms for an extra charge
High-speed Internet access (wired) is available in public areas for an extra charge
Area shuttle service is offered for an extra charge
We have included all charges provided to us by this hotel. However, charges can vary, for example, based on length of stay or the room you book.
Is the description of this hotel not correct? Tell us


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The reason for my post of this is because of my post on another thread last night. I was pretty angry at everything, so you'll have to excuse the F Bombs. If that offends you, then don't read it.
I swear to fucking god, how does someone deal with all this shit?
Aether, I apologize for ranting on your thread, but I don't know what or where to do/go to rant about this stuff.
Ah, fuck it. Nothing's helped and nothing will help. It would just be nice to have some modicum of understanding as to why people behave the way they do. Why they choose to forget what fucking scares them. Why the hell secrets make it ok to lie, ok to remain silent with knowledge. The damned 'ends justify the means' fucking BS. On and on.
It shouldn't be "Careful what you wish for." It should be, "Careful what you
wish for." Everything is so backasswards, it is amazing.
Fuck cliches. Fuck the ones that think they are awake, yet are beholden to their beliefs and biases, too fucking much of pansies to go experience and find out the truth themselves.
I am so angry right now. Fucking religions and sciences and capitalism and communism and fascism and tyrannies and oligarchies and all the fucking -isms.
God help us. It has saturated us all and has become a component of our souls. There is no magic revelation. There is no magic epiphany. There is no magic discovery. No freedom. No spiritual contentment. No future utopia. No future solution.
It is just us, and the assholes we are and will continue to be. Machinations. Usury. Politics. Corruption. That is power, and will continue to be. No catalyst, no change in philosophies, no magical mass spiritual awakening.
That is the truth of the human condition and truth is scalar. I'm so tired of people not seeing. And more tired of coddling just for usage of the one being coddled.
Please ignore this and thank you for letting me rant.
Septenary Man
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Today, my sister and her new husband invited our family and my fiancé over to look at their wedding pictures. Turns out, I caught the bouquet and flashed the world in the process. FML
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UW-Madison ranks No. 8 in Top-20 party school list
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Lili Answers for iPhone - iPadHome / iPhone - iPad /
Game Questions & Answers (Q&A) service lets you ask questions about video games for game consoles or PC games. So ask your Lili question for iPhone - iPad and get answers from real gamers or answer questions and share your insights and experience with the rest of the gaming community.
- Developer: Bitmonster Games
- Publisher: Bitmonster Games
- Genre: Role-Playing
- Release: Sep 21, 2012
- ESRB: Not Set
Question & Answers
Cannot find River spirit
How do you find the river spirit.
Answer from: B4mbi87
In rust grove. In the door that says 'Do not Disturb' go in there it's the stone fish looking over the bridge just to the left of the door that goes to Mill Hill.
Answer from: Sfcheri
I think I'm in the right place. I went through the Do Not Enter door. I've searched both sides of the bridge and the area near the door to Mill Hill. The only stone statue I found was to the right of the door and didn't respond to any taps. I think I have missed something. Can you be more specific about where to find the stone fish and what to do there?
Answer from: Sfcheri
Turns out there are two "Do not disturb" areas. Was in the wrong one. Once I went to the other one, the fishes obvious. thanks for the help.
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Shelli Steedman
member for 10 years, 9 months, 10 days
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This is me about 60 years ago. Add some gray hair and a couple of feet in height and it would be me today. I have been working on my family tree for about 30 years. I am a volunteer for two genealogical sites: RAOGK and GenGathering. If you would like genealogical help, email me at firstname.lastname@example.com.
Over the last few years, I have transcribed cemeteries in WA, OR, IA, and IN. I have also started taking headstone photos in many of the cemeteries in my area. If you need help w/ censuses, let me know.
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Contributions to
Find A Grave
23,175 Memorials Added
23,124 Memorials Managed
41 Memorials/Week
5,740 Photos
131 Photo Requests
12 Volunteer Photos Taken
11,282 Virtual Flowers
5 Fame Ratings
31 Sponsorships
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Messages left for Shelli Steedman (2)
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Angelic Long Wilmouth
RE: Ann Long George
Thanks Shelli! You have quite a few of my people but none are in the Find A Grave guidlines. Ann was my 3x great aunt. I really do appreciate how quick you are with all my edits lately. I have been doing genealogy over 25 years and now I do it professionally. I decided to completely re-do my tree and get as many on Find A Grave as possible and get the families connected.
Have a great weekend!
Added by
Angelic Long Wilmouth
on Jun 25, 2016 2:08 PM
RE: Mitchell City Cemetery
I tried to get the number from the phone book but can never find anything in them. The number for the city clerk is 812 849-3831. Perhaps they will direct you to the one you want. Best of luck to you.
Added by
on May 14, 2016 7:52 AM
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