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2011-08-16 10:27:00
Local police say a medical problem may have triggered a fatal crash involving a 71 year old La Pine man yesterday morning. Deschutes County Sheriff's officials say Robert Currin, 71, died when his SUV ran off of Burgess Road in La Pine. Witnesses said Currin's explorer suddenly left the road, going off the north side and coming to rest in dense willow bushes, less than two feet from the river. Currin was pronounced dead at the scene of the crash. He was wearing his seat belt at the time of the crash and the airbag did deploy.
King Solomon Lane closed between Ferguson Road and King David. (7/25)
Jones Road closed between Butler Market Road and Bennington Lane. (8/18)
Round-About Construction:
Murphy Road and Parrell Road closed with detours. (9/30)
Powell Butte Highway at Neff and Alfalfa Market Roads (8/31)
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The Early Offer is RecruitingNation's regular feature, giving you a daily dose of recruiting in the mornings. Today's offerings: With the newest edition of the BCS rankings released, we take a look at the biggest headlines of the top five teams from the recruiting trail.
Early Offer: Texas Tech springs surprise
835dESPN.com staff
Stidham pledge huge for Red Raiders
840dDamon Sayles
Video: Recruit comparison, Murray to Allen
838dESPN.com staff
Video: Teams expecting 2014 boost
840dESPN.com staff
Video: Comparing Garrett to Clowney
843dESPN.com staff
2015 OT Lanfear flips to Texas A&M
847dSam Khan Jr. and Max Olson
Early Offer: 2014's biggest climbers
848dJeremy Crabtree
Early Offer: Texas-sized recruiting battle
850dJeremy Crabtree
Texas lands DE Omenihu following visit
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Top position classes: Big 12
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Early Offer: The Dinkelmann dilemma
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Strong first impressions at junior day
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Strong, Texas hosting first junior day
851dMax Olson
Top overall position classes in 2014
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Video: Aggies' 2015 class starts fast
855dESPN.com staff


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Axel-In-Charge: Facing the 'Divided' Marvel NOW! Future
Comic Books
Comic Books
The Buy Pile
The Buy Pile
In Your Face Jam
Robot 6
Comics Should be Good
Robot 6
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Robot 6
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Jun 25, 09:15am
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Cupertino, CA
Unless you've been hiding under a rock, you might have heard about a little movie opening up tomorrow called "Iron Man 3." "Kiss Kiss, Bang Bang" writer-director takes control of the franchise's third installment with Robert Downey Jr. returning as Tony Stark, and expectations are very high after a strong marketing campaign and some very nice trailers.
With that in mind, today's CBR INK post is dedicated to all things Iron Man, and is nothing less than a massive gallery of Iron Man tattoos. Most of these were amassed via a Google Image search, so I don't have credits or stories for them, but hopefully the numbers will make up for the lack of details. We have everything from movie-inspired ink to tattoos based on War Machine and Adi Granov's covers — plus an Arc Reactor chest tattoo! See the full gallery after the jump.
Whenever I feel low, I shall look at these and go, "At least I didn't do that."
@Ajay. Die in a fire cunt.
Pedro Bear,
Really?! Ajay has a right to his opinion. I'm not a fan of tattoos. I look at these articles to see how well some inkers adapt comic book characters.
And yes, I would never do such a thing. I'm glad I didn't. That said, good for those that have such interesting and crazy homages.
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Now Michael Jackson's HOLOGRAM is hit with legal woes: British billionaire behind technology which wowed Billboard Music Awards claims it was used without his permission
- Hologram wowed audience singing Slave To The Rhythm in Las Vegas
- Hologram USA, owned by David Alki, says it was used without permission
- Company has now launched a lawsuit against Pulse, who put on the show
The moonwalking hologram of Michael Jackson that rocked the Billboard Music Awards last night has landed the show's organisers in a legal row after the British billionaire behind its technology claimed it was used without his permission.
The ghostly apparition of the King of Pop wowed spectators with a rendition of Slave To The Rhythm, a track taken from Jackson's posthumous new album Xscape, dancing about the stage in a golden jacket and red trousers as fireballs shot around him.
But despite the gimmick's apparent success at Las Vegas' MGM, Alki David - who owns Hologram USA - says his holographic technology was stolen, a claim organiser Pulse Entertainment's CEO John Textor denies.
Hologram USA holds the patents for the state-of-the-art technology with Musion Das Hologram Ltd.
Scroll down for video
Still rocking the house: A hologram of Michael Jackson performed at the Billboard Music Awards on Sunday
Judge Nancy J. Koppe of the Nevada court granted Alki last minute emergency access to the show - sparking angry backstage scenes as he and his team of lawyers clashed with MGM bosses.
During the heated confrontation, Alki alleged that a former Musion employee was discovered backstage and
that the court had been misled.
It is also understood that Musion believe the quality of the show would have been far more spectacular had they been involved.
The British billionaire: The controversial holographic representation of Jackson at the Billboard Music Awards at the MGM in Las Vegas went ahead despite a request for an injunction by media mogul Alki David's company Hologram USA
Mr David said: 'It is no surprise that Pulse was stealing our technology. I partnered with the inventor Uwe Maas to bring the magic back to entertainment and it's just such a shame that people like John Textor and his cronies felt it necessary to behave this way.'
Pulse were not immediately available for comment.
The onstage resurrection garnered a standing ovation by the massive star-studded audience, who were clearly moved by the performance nearly five years after his death.
However, only on Friday did a federal judge rule that the Billboard Music Awards could use the hologram, rejecting efforts from tech companies seeking to block the digital performance.
Judge Kent Dawson said there wasn't enough evidence to show the planned 3-D image would violate patents held by Hologram USA Inc. and Musion Das Hologram Ltd.
The companies own rights to technology known for digitally resurrecting deceased rapper Tupac Shakur at the 2012 Coachella music festival.
'The court's decision is not surprising,' attorney Howard Weitzman, who represented Jackson's estate and dick clark productions, wrote in an email. 'The request to stop this extraordinary Michael Jackson event was ludicrous.'
Plans to use the hologram during the show on Sunday emerged with the lawsuit, but were not confirmed until the hearing Friday afternoon. Show producers had been promoting only a 'history-making performance' at Las Vegas' MGM Grand Garden Arena that would promote Xscape.
Mesmerising: The incredible performance resulted in a standing ovation from the star-studded audience
Hologram USA and Musion said in their emergency lawsuit on Thursday that one of their products was being used without authorisation by a competitor to create a segment that depicts Jackson performing Slave to the Rhythm.
Attorney Michael Feder, representing the show and Jackson estate, filed a response on Friday, saying the holographic performance had been planned for months and was discussed with Alki David, who owns the rights to the technology that creates and projects lifelike images to appear alongside live performers through Hologram USA and Musion.
New track: The hologram performed Slave To The Rhythm, a new song off the posthumous album Xscape
Amazingly realistic: The hologram even did the moonwalk while dancers were beside it, and fire shot out around it
Plaintiff's attorney Ryan G. Baker said his clients were disappointed with the ruling, but the lawsuit will continue.
'It's only the very beginning of a case that will continue to be prosecuted by my clients, and ultimately they are confident that they will prevail and will recover all available damages for the defendants' infringing conduct,' Baker said.
Hologram USA obtained the rights to the patents after the bankruptcy of Florida effects house Digital Domain, which created the Shakur image to wide acclaim two years ago.
The lawsuit also named John C. Textor, the chairman of Florida-based Pulse Entertainment Corp. who was the former head of Digital Domain. Pulse is accused of using the hologram techniques without a proper license. A phone message left for Textor was not immediately returned.
Back in the day: Michael is seen here in February 1988. He died nearly five years ago, in June 2009
The lawsuit names Atlanta-based Pulse Entertainment Inc. as a defendant. Spokesman Ken S. Johnson said the company was listed incorrectly because it had no connection the Billboard Music Awards.
In March, Hologram USA sued Cirque du Soleil and MGM Resorts International over its show, Michael Jackson ONE at Mandalay Bay Hotel & Casino. The show features a performance by a digital rendition of Jackson, which the company also contends is an unlicensed use of its technology.
The case is being handled in a Los Angeles federal court and Cirque du Soleil and MGM Resorts have been granted an extension until May 23 to respond to the lawsuit.
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For his seventh album, Deadmau5 has turned from an electro-house polymath into the world's most unnecessary Nine Inch Nails tribute act. With a running time longer than the new X-Men movie, the two-disc While(1<2) gives Mau5 plenty of room to spiral downward into many of Trent Reznor's favorite tricks – the moody minor-key melodies, the Erik Satie-derived piano miniatures, the flickering static and machine fuzz, the bent and tortured notes, the plodding chug on acoustic guitar. Twice you can hear him end a song by taking his foot o the piano's sustain pedal, just like NIN collaborator Aphex Twin's "Avril 14th." There's even a remix of a seven-year-old Nine Inch Nails song ("Survivalism") that makes it sound like it came from 1999. However, erase the Reznor fan fiction from this 141-minute behemoth, and there's a solid 64-minute house record hidden in there, trading in night-cruise synths and walloping throb. Highlights like "Infra Turbo Pigcart Racer" and "Mercedes" are revving engines that gleam with vintage textures: Deadmau5 shines brightest, it seems, when he turns off The Social Network and puts on Drive.
- While(1<2)
- Deadmau5
- Mau5trap/Astralwerks
From The Archives Issue 1212: July 3, 2014
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Dino Morea tweeted using "# CelebratingJio" so that people think he is also a celebrity. Lol...
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"Chasing Eternity"...
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This week, it's time to get a little wicked at BlogAdda! No, we're not suddenly turning into a grey character, it is just because we have Bhakti Menon of Wicked Spoon Confessions for company! A food and travel blog with a twist, WSC celebrates the two biggest joys of life - food and travel - with a twist! It is a blog where burgers and beer live happily together, a place which you leave with knowledge, recipes and above all, a love for food! We caught up with Bhakti Menon for some confessions, and this is what we came up with during this fun and foodilicious interview!
Travelling to a spiritual destination is like embarking on two journeys in one go.
When one decides to take the road less travelled by. Every turn and every project brings new challenges.
"A Murder She Saw"
"Why wings?"
"Lucky Dad, Lucky Us"
Blog about how to take the message of #ShareTheLoad to the next generation! Tell us ways in which you can raise the next generation with the right values of gender equality in the household, and no scope of gender stereotypes.
All of us are in an invisible race today, running towards money, fame, stability and trying to check off items in our 'must want' list. But as they say, when you look back at your life, you will remember the time you spent with your loved ones, and not the figure you've accumulated in your bank account. Memories that you make with your loved ones are the true treasure that you accumulate through your life. And we want you to share these #MemoriesForLife with us through a blog post!
It's that time of the year again, where we will scream and shout, "Holi Hai"! As India's most colourful and fun-filled festival is back, gulaal will blast from pichkaris around every corner, delicious gujiya will be washed down with thandai, and everyone will jump and dance with 100% masti!
For E-commerce bloggers, it is essential to define your audience and take worthy steps to reach out to them. This will be the sole determinant of your marketing plan.
Picture talk: Use of images attracts more eyeballs. It is humanitarian. Even if it is a data centric post or a theoretical one, have an image that speaks significantly for your post.
Content is the king - In the blogging world so diverse and big, you need to establish yourself with a distinction. the only road to it is good, original and valuable content. Be creative, different and surprise your readers once in a while.
The clouds have finally dropped their anchors over the nation and waking up to a musical pitter patter is a beautiful routine now. The sun seems to be enjoying a vacation after months of overwork.. Overall, the days have gotten prettier, work doesnÆt seem that frustrating, walks on sidewalks seem to be fun and evenings have taken on a mushy tone. When such a beautiful time coincides with an impending weekend, perhaps thatÆs heaven on earth. As the raindrops on the roof create their own symphonies, we are sure you might be thinking of sitting down with a steaming pakoras and chai, writing a monsoon inspired post.
Our favourite friend, Friday, is back after what seems like eons, and we couldn't be happier! Life is back to being beautiful with long spells of time to check things off your weekend wishlist. As you finally enter your own world of fantasies breathing in serenity around you, we are here to rev up your thoughts a little.
During our time in this world, there are unnoticed moments that actually hold the power to drive our lives into a completely different course, opening doors that we might not have ever thought of. Yes, this might be a little dose of philosophy, but how many of you have actually tried connecting the dots to the minuscule events that catalyze the biggest events? A journey, a meeting or a random encounter - all of these have the power to change our lives, even when we don't see the hand of destiny behind such things.
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Why isn't there a better trans role model? Caitlyn Jenner is nothing more than an attention whore, and she doesn't even believe in gay marriage! Plus,


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We usually meet on the first Sunday of the month but because of schedule conflicts we are going to meet on June 8 at my house. I will have bagels and cream cheese, maybe some donuts, some fruit, and juice. If you need coffee bring it. We have a coffee maker but I don't know how to use it.
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Just create a new account—we'll guide you to the flip side.
Hi there! Thanks for visiting PokéCommunity. We're a group of Pokémon fans dedicated to providing the best place on the Internet for discussing ideas and sharing fan-made content. Welcome! We're glad you're here.
In order to join our community we need you to create an account with us. Doing so will allow you to make posts, submit and view fan art and fan fiction, download fan-made games, and much more. It's quick and easy; just click here and follow the instructions.
So much blue in your VM wall.
You, my friend, are a genius.
Sorry about that, Drew! D;
Well, it's cute and green. Making it super awesome. :3
I approve of anything green XD
I thought you'd approve~ :D
Cute :D
I KNOW WHAT YOU HAVE TO TALK TO ME ABOUT. I await the conversation. :>
God, it needs to go away! I'll talk sternly to it when you come back and try to make it go away. XD
Is what you have to talk to me about good or bad? .. Byeeee~
Well, I'm good around here, still with a bit of a cold but well XD
I have to talk to you about something. Maybe later when I come back, I have to go now.
Weeeeeeeentley. It's going fine; everything at school has cooled off. :>
How are things with you?
Fiiiiiii how is it going? :D
B-b-but, I didn't mean anything by it! D:
Mhm. Private and independent. >>;
Actually my fear is that I'll get demoted for that, precisely XD
That's why these VM's make me feel bad :x
All-girls school?
Oh, don't get het up, Wentley. I was only jokin'. D:
You can deal with reports. But tell me, 'cause I thought I'd been demoted or something. XD;


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Not Just Shirts Online Store > Egghead Critters > Pirate Egghead > Pirate Egghead Stein
An egghead wearing a pirate hat and an eye patch and holding a flag is a funny gift design for pirate lovers, cooks and kitchen raiders at birthdays and holidays.
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J.Wong's Thai & Chinese Bistro
Conventions and trade shows can be a great time and a welcome change of pace from the office, but they can also be exhausting—the result of countless conversations, over stimulation, and spending too many hours on your feet. After such a day, delicious comfort food in a relaxed setting is exactly what you need, and that's what you can expect at J.Wong's Thai & Chinese Bistro.
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Software Engineering Jobs in West Yorkshire
Jobs 1 to 15 of 40
Contract Java Developer Leeds 6 months (with likely extension)
Contract Java Developer: Key Skills and Background. 5 + years software engineering experience across multiple industries. Java with experience of at least one other language... Experience of working in an Agile environment... Ideally degree educated in an software engineering related discipline or equivalent proven working experience... Contract Java Developer: About the...
Leeds, West Yorkshire -
Hotfoot Recruitment
Rate: From £350 to £450 per dayPosted: 20 hours ago
Software Engineer
C# / .NET / VB.NET / T-SQL / SQL Server... You will need to have previous development experience and strong skills in C# / .NET / VB.NET / T-SQL / SQL Server / IIS... To join our Leeds office we require a driven Software Engineer with existing skills in C# / .NET / VB.NET / T-SQL /...
Leeds, West Yorkshire -
Salary: £35000 - £45000 per annum + BonusesPosted: Yesterday
Software Developer/Software Engineer/ Software Programmer 30k - 40K
You will be a technically minded hands on developer with a passion for technology and mentoring junior developers... Whether it be dealing with external/internal websites, web applications or even mobile app's you will have a great variety of work. You will have the following skills: ASP.Net. C#...
Leeds, West Yorkshire -
Evolution Recruitment Solutions
Posted: 2 days ago
Lead Full Stack Software Engineer
Our client, a Software Consultancy based in Leeds are looking for a Full Stack Software Engineer to join their rapidly growing team... As well as being a strong, hands on software developer you will also be comfortable working with Lean delivery methods in an Agile environment and within a Devops...
Leeds, West Yorkshire -
Hotfoot Recruitment
Salary: £60,000 per annum + 10% BonusPosted: 2 days ago
Contract PHP Developer
As well as being a strong, hands on software developer you will also be comfortable working with Lean delivery methods in an Agile environment and within a Devops culture... Primarily focused on hands-on engineering of consumer and business related applications with an expert understanding of software engineering best practices.....
Leeds, West Yorkshire -
Hotfoot Recruitment
Rate: From £350 to £450 per dayPosted: 22 hours ago
Graduate Software Developer
C# / Object Orientated Developer / Software Engineering / Maths... * Open to Maths, Computer Science or Engineering Grads... Using your C# / Object Orientated Developer / Software Engineering / Maths experience you will be a valued member of our team... WE NEED YOU TO HAVE… * Degree in Maths or Computer Science or Engineering... * Knowledge of C#,...
Leeds, West Yorkshire -
Salary: £22000 - £23000 per annumPosted: Yesterday
Principal Developer / Development Manager
Are you a talented leader of software engineers who wants to work for an innovative and progressive company? Do you have a passion for applying lean and agile ways of working to continually improve product development teams? We`re developing integrated and innovative software solutions which are helping NHS clinicians...
Leeds, West Yorkshire -
EMIS Group
Salary: CompetitivePosted: 17 days ago
.Net Developer - C# and/or VB.net - Leeds
The Software engineer / .Net Developer will; Design and develop software solutions for Xuber's core products... The Software Engineer / .Net Developer should have experience in the following; C# and/or VB.NET development... Ideally 2-3years or more commercial SQL experience (either SQL Server or Oracle)... Experience of developing multi-...
Leeds, West Yorkshire -
Salary: CompetitivePosted: 7 days ago
Senior/Lead .NET Developers (C#) Home Working/Flexible Hours
C#.NET Developers required for prestigious Leeds company... You will ideally already be a Senior/Lead C#.NET Developer, but what is key to my client is not that this person has every single technology that they use currently, but that they have the right attitude, know where they have...
Leeds, West Yorkshire -
Hotfoot Recruitment
Salary: From £30,000 to £50,000 per annumPosted: 2 days ago
Ruby on Rails Developer - Tech Start Up - Remote Working
This role will see you lead a talented team of software engineers as the company deliver a new web platform using Ruby on Rails... ** Agile / Test Driven Development. ** Experience of leading / managing software engineering teams. ** Software Architecture & Design experience using Ruby. ** Experience of Sinatra or associates Ruby frameworks would looked...
Leeds -
Cathcart Associates
Salary: £35000 - £55000 per annum + benefits + remote workingPosted: 16 days ago
Software Developer/Software Engineer/ C#.Net Developer 30K-40K+Bens
Software Developer/Software Engineer/ Software Programmer - C#, .Net Developer £35,000 - £41,000 + excellent benefits including private healthcare and generous pension based in Wetherby. A fantastic opportunity in Wetherby for a software house and Microsoft Partner Company in a leading business dealing in procurement services are currently hiring... Essential Skills:...
Wetherby, West Yorkshire -
Evolution Recruitment Solutions
Salary: 30,000 - 40,000Posted: 2 days ago
Java Software Engineer
My client based in Central Leeds are currently recruiting for a Java Software Engineer... - Strong Object Oriented skills. - Good database knowledge of SQL or NoSQL... logic, data structures, algorithms, low-level architecture, systems infrastructure, network stack, etc. - Exposure to continuous integration, build tools and scripting. - Use of source code version...
Leeds, West Yorkshire -
The Bridge Ltd
Salary: Up to £55,000 per annum plus benefitsPosted: 3 days ago
Software Developer/Software Engineer/ .Net Developer ASP C# 35K-40K
Software Developer/Software Engineer/ Software Programmer - C#, .Net Developer £35,000 - £40,000 + excellent benefits based in Leeds... The company is Microsoft certified partner, so great for working with latest technologies!! Essential Skills: ASP.Net MVC. HTML CSS JavaScript jQuery. C# SQL... The also offer technical and project management consultancy.
Leeds, West Yorkshire -
Evolution Recruitment Solutions
Salary: 35,000 - 40,000Posted: Yesterday
C#.Net Software Developer
An exciting opportunity has arisen for a C#.Net Software Developer, based at my client's Bradford Head Office... As a Software Developer you will be responsible for: - .Net development, using mainly C#, but other languages where appropriate... - Degree in relevant subject e.g. Computer Science, Software Engineering etc or...
Bradford, West Yorkshire -
The Bridge Ltd
Salary: Up to £40,000 per annum plus benefitsPosted: 3 days ago
Software Developer
Software engineer: C#, VB.net, Winforms, SQL paying £350 a day... - Excellent knowledge of using database systems (SQL Server, MySQL, SQLite etc) - Working knowledge of backend / web applications development in ASP.Net or PHP... - Good understanding of web services (SOAP, XML), e-commerce payment/ticketing systems and SSL... Software engineer/...
West Yorkshire -
Premier IT
Posted: Yesterday
Software Engineering Jobs in West Yorkshire


text:
"Come to me all you who are burdened
and I will give you rest"
Here I am, Lord.
I come to seek your presence.
I long for your healing power.
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"Come to me all you who are burdened
and I will give you rest"
Here I am, Lord.
I come to seek your presence.
I long for your healing power.
Your death on the cross has set me free.
I can live joyously and freely
without fear of death.
Your mercy knows no bounds.
I remind myself that I am in the presence of the Lord.
I will take refuge in His loving heart.
He is my strength in times of weakness.
He is my comforter in times of sorrow.
But one of them, Caiaphas, who was high priest that year, said to them, 'You know nothing at all! You do not understand that it is better for you to have one man die for the people than to have the whole nation destroyed.' He did not say this on his own, but being high priest that year he prophesied that Jesus was about to die for the nation, and not for the nation only, but to gather into one the dispersed children of God. So from that day on they planned to put him to death. Jesus therefore no longer walked about openly among the Jews, but went from there to a town called Ephraim in the region near the wilderness; and he remained there with the disciples.
How has God's Word moved me?
Has it left me cold?
Has it consoled me or moved me to act in a new way?
I imagine Jesus standing or sitting beside me,
I turn and share my feelings with him.
I thank God for these few moments we have spent alone together and for any insights I may have been given concerning the text.
If you appreciated the daily prayer or have any suggestions or insights we will be glad to hear from you.


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Chicago, ILUSA
January 21, 2014
I enjoy live theater and all forms of make-believe, but have trouble motivating myself to choose what to see.
Playing around on line.
Previous lives as linguist, ESL teacher and immigration specialist; now either retired or unemployed (not sure yet).
Great Thanks Scott
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text: AdChoices Terms of Use Privacy Policy Hide TOP 10 Top 10 Better Man New York vs. Los Angeles Share Tweet 0 Shares 1. Employment Prospects USACE HQ / Flickr I admire the struggles and sacrifice of New Yorkers. But I didn't need 10 years of it. If you're not working long hours, you're hustling. If you're not hustling, you're unemployed. I have a NYC friend with a master's degree in architecture, and she manages those guys who scan your tickets at the Empire State Building. In L.A., you can struggle and still make a shi*load of money (actor/waiter) or struggle and still have a nice apartment (with pool). But best of all, the economy here is recession-proof. While major industries in NYC are getting hit by a recession bus (publishing, finance, etc.), L.A.'s notorious entertainment industry has hardly taken a licking. L.A. has also been getting some love from the tech industry (powerhouses like Google and YouTube recently opened up major offices), creating more jobs for those looking for opportunity. TOP 10 9 8 7 6 5 4 3 2 1 Comments Share your opinion Your name More Like This Top 10 Better Man 10 Bedroom Lessons Your Younger Self Can Still Teach You Read More Top 10 Better Man We Debunk The 10 Most Ridiculous Myths People Believe Read More Top 10 Dating Don't Consider Yourself A Real Man Unless You Can Do These 10 Things Read More Top 10 Entertainment Drones Are About To Change Your Life In Ways You Never Imagined Read More Top 10 Travel 10 Honeymoon Destinations That Will Win You Brownie Points For Life Read More Top 10 Dating Sex Can Help You Live Longer, According To Science Read More Around the Web Special Features Dating Tips A Guide To Hooking-Up Online Read More AskMen Deal Alerts Get Daily Deals You Actually Want Read More


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IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards,
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A generic procedure can be specialized, by compilation through views, to operate directly on concrete data. A view is a computational mapping that describes how a concrete type implements an abstract type. Clusters of related views are needed for specialization of generic procedures that involve several types or several views of a single type. A user interface that reasons about relationships between concrete types and abstract types allows view clusters to be created easily. These techniques allow rapid specialization of generic procedures for applications
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UD: A 'Best Kept Secret' No Longer
The Irving Community Television Network recently featured UD and President Thomas W. Keefe.+ Read More
Launched in 2002, the Dallas Medieval Texts and Translations series is engaged in an ambitious long-term project: to build a library of medieval Latin texts, with English translations, from the period roughly between 500 and 1500, which will represent the whole breadth and variety of medieval civilization. Thus, the series is open to all subjects and genres, ranging from poetry and history through philosophy, theology, and rhetoric to treatises on natural science. It will include, as well, medieval Latin versions of Arabic and Hebrew works. Placing these texts side by side, rather than dividing them in terms of the boundaries of contemporary academic disciplines, will, it is hoped, contribute to a better understanding of the complex coherence and interrelatedness of the many facets of medieval written culture.
Dallas Medieval Texts and Translations is made possible through a transatlantic collaboration between the University of Dallas, which houses the editorial offices of the series, and Peeters of Louvain, which produces it in handsome volumes that are designed to last.
As of June 2013, nineteen volumes have been published; about twenty more are under contract.
For more information, please go to www.dallasmedievaltexts.org.
The Irving Community Television Network recently featured UD and President Thomas W. Keefe.+ Read More
The mosaic seal in Haggar University Center. The stone sign on Northgate. The marble statue of Mary outside the Church of the Incarnation. These various pieces that enrich and beautify our campus, largely taken for granted by everyone who comes after as things that must always have been here, were actually gifts from graduating classes through the years.+ Read More
The University of Dallas has announced the appointment of two new members to its board of trustees. Terry O'Halloran, MBA '83, and Julie Weber, BA '91, who recently attended their first full meeting in May, are both University of Dallas alumni.+ Read More


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Phone: (919) 549-8411
The International Society of Automation (www.isa.org) is a nonprofit professional association that sets the standard for those who apply engineering and technology to improve the management, safety, and cybersecurity of modern automation and control systems used across industry and critical infrastructure. Founded in 1945, ISA develops widely used global standards; certifies industry professionals; provides education and training; publishes books and technical articles; hosts conferences and exhibits; and provides networking and career development programs for its 40,000 members and 400,000 customers around the world.
ISA owns Automation.com, a leading online publisher of automation-related content, and is the founding sponsor of The Automation Federation (www.automationfederation.org), an association of non-profit organizations serving as "The Voice of Automation." Through a wholly owned subsidiary, ISA bridges the gap between standards and their implementation with the ISA Security Compliance Institute (www.isasecure.org) and the ISA Wireless Compliance Institute (www.isa100wci.org).
Membership Benefits
Full ISA Membership
Automation Affiliate Membership
Student Membership
Earned Membership Grades & Reciprocal Memberships
Monday, 27 Jun 2016 - Friday, 01 Jul 2016
Charlotte, NC,
Friday, 01 Jul 2016
Charlotte, NC,
Monday, 01 Aug - Thursday, 04 Aug, 2016
Lake Buena Vista, FL,
ISA members have access to dozens of unique benefits. Update your member profile, set your communications preferences, find products and services that are right for you, and more – all from the convenience of your ISA website.
Featured Training: ISA Boot Camps
ISA System Wide Awareness Training (S.W.A.T.)
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ISA to develop a new certification program as part of a $23 million grant to fund degree programs and prepare workers for careers in "Mission Critical Operations"
Cover story: Industry 4.0: Intelligent and flexible production
Monday, 01 Aug - Thursday, 04 Aug, 2016
Wyndham Lake Buena Vista Resort,
Thursday, 18 Aug 2016
Space Center Houston,
Saturday, 24 Sep - Monday, 26 Sep, 2016
Newport Beach Marriott Hotel & Spa,
Wednesday, 12 Oct 2016
ISA - International Society of Automation,
Early bird registration deadline for ISA's 2016 Water/Wastewater and Automatic Controls Symposium extended to 1 July
ISA releases preliminary program for its 59th Power Industry Division (POWID) and EPRI Symposium
Steven D. Drew announced as keynote speaker for ISA's 2016 Water/Wastewater and Automatic Controls Symposium
ISA100 Wireless Compliance Institute evaluating nominations for the 2016 Excellence in Automation Award
Yokogawa Electric Corporation has certified the F9092RB, an updated and improved ISA100 Wireless stack implementation
Honeywell Launches ControlEdge Programmable Logic Controller
Honeywell Introduces Uniformance Suite as Industrial Internet of Things (IIoT) Analytics Platform
Honeywell Launches Industrial Internet of Things-Ready Gas Measurement and Data Management Solutions for Natural Gas Utilities
Honeywell Introduces Matrikon OPC UA Software Development Kit
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ISA has the resources and expert networks that you need – no matter what position you hold in our industries. Browse the products and services designed to meet the needs of these common industry roles:
ISA gives me the chance to work with science and engineering students in my community. Each year, my section presents an award to a middle school team in the area who compete in a national engineering competition. I feel it's my responsibility to encourage young people to explore careers in automation and engineering.
-Peter Baker, ISA Twin Cities Section member
ISA's products and services are organized by technical topic – a selection of technologies, trends, and areas of focus that are relevant to your on-the-job performance:
ISA Certified Automation Professional® (CAP®) certification is a mark of career excellence that affirms your commitment to quality and demonstrates your expertise and knowledge of state of the practice. Most important, it is acceptance and recognition from your peers.
The CAP program requires that you recertify every three years to maintain your certification. The purpose of recertifying is to ensure that as a CAP-certified professional you maintain the same level of knowledge originally demonstrated when you passed the written examination. If you do not recertify within four (4) months after your expiration date, your certification is considered lapsed and you must reapply and retest.
Send your completed Renewal Application and renewal fee to ISA 60 days before your expiration date to keep your CAP certification active.
You will receive notices (these may come in the form of mail, email, and phone) from ISA when your renewal date approaches, but it is your responsibility to submit your renewal on time. Your certification expires on the date printed on your certificate. Make sure to keep your contact information up-to-date in your ISA account.
If you do not send in your renewal application showing your 150 PDPs within 4 months after the end of your three-year certification period, your certification will expire.
If your certification has been expired for four months or longer, regaining Active Status is only possible by reapplying as a first-time applicant and meeting the work, education, and exam requirements.
If you choose to renew by exam, you must complete the Renewal Application and submit payment ($330 ISA Members; $445 Affiliate Members/Community Members/ List). Once your application is processed, you will receive instructions for scheduling your exam with Prometrics.
To renew by Professional Development Points (PDPs), you must 1) complete the Renewal Application and submit payment ($205 ISA Current Members; $320 Affiliate Members/Community Members/ List) and 2) submit your CAP CDP Activity Record.
On 1 March 2012, ISA implemented the new ISA CAP Continuing Professional Development Program. If your CAP certification renewal date is before 1 March 2013, you can continue using the Original PDP requirements for renewal or opt to use the new ISA CAP CDP Program requirements for renewal. For more information on the Original PDP requirements, click here.
Please note that if you are following the Original PDP requirements you do not need to submit a submit a CAP CDP Activity Record with your renewal application. You should submit the completed renewal application with payment. If you choose to follow the new CAP CDP Program, you must submit a CAP CDP Activity Record along with a completed renewal application (do not complete page 2 of the application) with payment.
The ISA Certified Automation Professional® (CAP®) Continuing Professional Development (CDP) Program and its Professional Development Points (PDPs) requirements are designed to be as dynamic as the automation profession the CAP certification program represents and credentials and to assure and enforce continued, relevant professional development in our dynamic professional context.
Professional Development Points (PDPs) are used as a common denominator because there is such a broad range of educational and professional opportunities relating to automation/instrumentation in which credits for professional development activities can be obtained. The following text will define the PDP equivalencies of the various accepted activities.
The CAP CPD Program institutes a PDP reporting process that includes minimum professional development benchmarks. These minimum requirements do not constitute nor do they imply a competency level that is adequate for all certified individuals. To maintain relevancy and competency, it is recommended that you assess your own professional development needs while planning and executing your unique CPD program as you practices in the automation profession. In assessing your individual needs, input from mentors, supervisors, and colleagues may be helpful.
Download the ISA CAP CPD Program Document
Your CAP CPD Activity Record must include the following information for each CPD activity submitted for certification renewal:
Note: For audit purposes, you must have and be able to provide upon request documentation for all recorded activities claimed for renewal.
Active professional practice in automation is a significant factor in maintaining and improving your knowledge and skills. You may be an actual automation practitioner or one that is "influencing" the practice of the automation. In this context, "influencing" means having some effect on how the automation profession is practiced without necessarily performing technical work (i.e., management, teaching).
Professional Practice is further defined as:
Refer to the Professional Development Points (PDPs) Summary table below for PDPs allowed for activities in this category.
Formal Automation-related Activities should be included in your CPD program. Formal Automation-related Activities are typically educationally associated and may include an exam or other evaluation process. When no evaluation process is offered, credit may still be claimed in this category for activities that are over four hours in length. Non-evaluated activities of less than four hours should be categorized as Informal Automation-related Activities (see the next section, below).
Examples of Formal Automation-related Activities include:
Unlike Formal Automation-related Activities, Informal Automation-related Activities are shorter in duration and do not involve any evaluation process.
Examples of Informal Automation-related Activities include:
Participation activities are those that promote peer interaction and provide exposure to new ideas and technologies. These activities enhance the automation profession and serve the public interest.
Examples of Participation activities include:
Eligible presentations are those of a technical or professional nature that are discretionary (outside of your normal job functions). Presentations must be developed by the CAP receiving the PDPs. Presentations developed by others will not qualify for PDPs.
Examples of Presentations include:
Refer to the Professional Development Points (PDPs) Summary table below for PDPs allowedfor activities in this category.
Contributions to the Automation Body of Knowledge activities are those that expand or develop the technical knowledge base of the automation profession.
Examples of Contributions to the Automation Body of Knowledge activities include:
You must earn 150 PDPs total across three of the categories below during your three-year certification period. You may not exceed the maximums allowed in each category.
Instructor for an automation-related training course/seminar over four hours in length
2 PDPs x Number of Contact Hours with Students
Additional Certification/License in related areas
Each certification/license = 2 PDPs
General Informal Automation-Related Activities
1 Hour = 0.5 PDPs
Membership/Participation in a Professional Society
Each Membership = 2 PDPs
Each National Society Meeting Attended = 2 PDPs
Each Local/Regional Society Meeting Attended = 1 PDP
Each National Appointed or Elected Position Served = 2 PDPs
Each Local/Regional Elected Position Served = 1 PDP
Each Local/Regional Appointed Position Served = 0.5 PDPs
Delivery of a Paper to an Audince at a Scheduled Meeting
10 PDPs x Number of Deliveries (for published papers)
Delivery of a Paper to an Audience at a Scheduled Meeting
5 PDPs x Number of Delivieries (for non-published papers)
Development of automation-related codes and standards
1 Hour = 0.25 PDPs
Patents (claim once per patent)
Each Patent = 10 PDPs
Publication of reviewed papers
Each Paper = 10 PDPs
PhD/Master's Thesis
Each Thesis = 20 PDPs
Book Publication
Each Book = 40 PDPs over two years
Publication of non-reviewed papers (limit 2/year)
Each Paper = 5 PDPs
Article Review (limit 10 per year)
1 Article = 1 PDP
Paper Editing (limit 5/year)
Each Paper = 2 PDPs
Reviewing of Papers/Presentations for Technical Conference (limit 10/year)
Each Reviewed Paper/Presentation - 1 PDP
Serving in Program Committee for Technical Conference (limit 2 terms/year)
Each Term of Active Service = 5 PDPs
If you do not work/practice full time, you might not be able to meet the requirements of the CAP CPD Program as readily as those who are practicing full time. If this describes your situation, you can submit a written request to the CAP Steering Committee for special consideration to have your CPD PDP requirements reduced. If the Committee grants a reduced requirement, you must submit an Annual Reduced-PDP Submittal Form.
Recognizing that circumstances such as unemployment, illness, etc. cannot be anticipated, the request should be accompanied by a report of current PDPs acquired and anticipated PDPs for the remainder of your certification term. Your individual circumstances will influence what your reduced CPD Program requirements will be. If granted reduced CPD PDP requirements, you will generally be expected to accumulate at least 30 PDPs per year. Upon return to full-time professional practice, you must comply with the full requirements of the CAP CPD Program.
If you feel you were wrongly denied credit for a CPD activity, then you have the right to appeal. For information about the appeal process, please click here.


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Metabolomics, Metabonomics and Metabolite Profilingby William J Griffiths
The completion of gene sequencing has resulted in an intensified investigation of the proteome and metabolome. Metabolite profiling methods used for disease diagnosis have been expanded with the advent of new technology and are being applied extensively in the quest for the discovery of new markers for diseases. In this comprehensive resource, the Editor draws
- Overview
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The completion of gene sequencing has resulted in an intensified investigation of the proteome and metabolome. Metabolite profiling methods used for disease diagnosis have been expanded with the advent of new technology and are being applied extensively in the quest for the discovery of new markers for diseases. In this comprehensive resource, the Editor draws together experts from the field and provides an insightful introduction into the technology and methodology. Metabolomics, Metabonomics And Metabolite Profiling covers a broad range of topics including:
• Mass spectrometry and NMR in metabolite profiling
• Current applications of metabolite profiling for disease diagnosis
• Studies of specific metabolite classes
• Plant metabolites
• Metabolite data mining
• Global systems biology This book is a must have, up-to-date, reference which will appeal to academics, students, technicians and professionals working in, or joining this field.
Editorial Reviews
Product Details
- 9780854042999
- Publisher:
- Royal Society of Chemistry, The
- Publication date:
- 10/28/2007
- Series:
- RSC Biomolecular Sciences Series, #9
- Pages:
- 336
- Product dimensions:
- 6.40(w) x 9.30(h) x 1.00(d)
Read an Excerpt
Metabolomics, Metabonomics and Metabolite Profiling
By William J. Griffiths
The Royal Society of ChemistryCopyright © 2008 The Royal Society of Chemistry
All rights reserved.
Mass Spectrometry for Metabolite Identification
The School of Pharmacy, University of London, 29–39 Brunswick Square, London WC1N 1AX, UK
1.1 Introduction
Mass spectrometry (MS) and nuclear magnetic resonance (NMR) constitute the two major pillars upon which the disciplines of metabolomics and metabolite profiling are built. Both these techniques have their advantages and disadvantages, but the fundamental difference in the nature of their spectroscopy means that they provide complementary information to the analytical scientist. NMR spectroscopy is discussed in detail in Chapter 2, while this chapter will concentrate on mass spectrometry and associated methodologies appropriate in metabolomics research. The principles of mass spectrometry will be described and examples of metabolite analysis given. As the range of metabolite structures present in biology (almost) exceeds the imagination, we will take many examples from the class of biomolecules that we have been most intimately involved with, i.e. sterols and steroids. However, many of the references quoted are equally applicable to other area of metabolite profiling and metabolomics.
1.2 Mass Spectrometry
1.2.1 Principles
Simplistically, a mass spectrometer consists of an ion source, a mass analyser, a detector and a data system (Figure 1.1). Sample molecules are admitted to the ion source, where they become ionised. The ions, which are now in the gas phase, are separated according to their mass-to-charge ratio (m/z) in the mass analyser and are then detected. The resulting signals are transmitted to the data system and a plot of ion abundance against m/z corresponds to a mass spectrum. In many cases, a separating inlet device precedes the ion source, so that complex mixtures can be separated prior to admission to the mass spectrometer. Today, the separating inlet device is usually either a capillary gas chromatography (GC) column or a high-performance liquid chromatography (HPLC) column, although capillary electrophoresis and thin-layer chromatography can be interfaced with mass spectrometry.
For metabolite analysis a number of different types of ionisation methods are used to generate gas-phase ions and these include: electron ionisation (EI), chemical ionisation (CI), electrospray (ES), atmospheric pressure chemical ionisation (APCI), atmospheric pressure photoionisation (APPI), and the recently introduced, desorption electrospray ionisation (DESI) technique. Other ionisation techniques used, but to a lesser extent, are liquid secondary ion mass spectrometry (LSIMS) and fast atom bombardment (FAB), or for more specific applications, matrix-assisted laser desorption/ionisation (MALDI) (see Chapter 9) and desorption ionisation on silicon (DIOS). The most widely used ionisation modes are discussed below, as are the chromatographic devices to which they are interfaced.
1.2.2 Ionisation
22.214.171.124 Electron Ionisation (EI) and Chemical Ionisation (CI)
Historically, the most important method of ionisation of small biomolecules (< ~500 Da) is EI. The effluent from a GC column is readily transferred to an EI source, thereby allowing the combination of the high separating power of a GC column with mass analysis. EI involves the bombardment of gas-phase sample molecules (M) with high-energy electrons (e-), usually of 70 eV energy; the result is the generation of [M]+ ions which are usually radical cations, and thermal energy free electrons (e-) (eqn 1).
In many cases the molecular ions, [M]+, are unstable and fragment to generate more stable products (eqn 2).
Fragmentation upon EI can be seen as both advantageous and disadvantageous. On the plus side, the fragmentation pattern resulting from decomposition of a molecular ion can provide structural information, allowing its identification. On the negative side, however, fragmentation may be so extensive that the molecular ion may not be observed, and thus the molecular weight of the compound of interest not determined. EI can be used to generate either positive ions [M]+or negative ions [M]+-. Negative ions are generated via an electron capture event, which involves the capture of secondary low-energy electrons generated by ionisation of a bath gas (e.g. Ar, N2) (eqn 3).
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A prerequisite of EI is that the sample to be ionised must be in the gas phase; this is also true for GC and has led to the extensive development of derivatisation chemistry to allow the vaporisation of many small biomolecules without their decomposition.
CI is a close relative of EI. It differs in that analyte ionisation is achieved via proton attachment rather than electron ejection (positive ion). In CI the ion source contains a reagent gas, often methane, which becomes ionised by EI and acts as a proton donor to the analyte (eqn 4).
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The resulting ion, [M + H]+, is an even-electron protonated molecule, which is more stable than the equivalent odd-electron molecular ion, [M] +, formed by EI, and thus fragments to only a minor extent.
Electron-capture negative ionisation (ECNI), also called electron-capture negative chemical ionisation (EC-NCI), exploits the electron capturing properties of groups with high electron affinities (eqn 5). The method often utilises fluori-nated agents in the preparation of volatile derivatives with high electron affinities. For example, trifluoroacetic, pentafluoropropionic or heptafluorobutyric anhydrides can be used to prepare acyl derivatives of amines and hydroxyl groups, perfluorinated alcohols can be used to generate esters of carboxylic acids, while carbonyl groups can be converted to oximes which can then be converted to pentafluorobenzyl oximes or pentafluorobenzylcarboxymethoximes, for example (Scheme 1.1). Ionisation proceeds with the capture of a secondary low-energy electron generated under CI conditions, by the high-electron affinity fluorinated groups. Ionisation may lead to the formation of stable [M]- ions, or it may be dissociative, depending on the analyte and the derivative used. The major advantage of EC-NCI is that ionisation is specific to compounds containing the electron capturing tag and provides excellent sensitivity in terms of signal to noise ratio when either the stable [M]- ion or a negatively charged fragment ion is monitored.
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It is of historical interest that HPLC has been combined with EI and CI, but as both of these ionisation modes require high vacuum, the necessary removal of HPLC solvent has made this combination difficult. The natural marriage for HPLC is with atmospheric ionisation (API) methods discussed later.
188.8.131.52 Liquid Secondary Ion Mass Spectrometry (LSIMS) and Fast Atom Bombardment (FAB) Ionisation
It can be argued that the introduction of the FAB method of ionisation by Barber and colleagues in 1981 initiated the revolution in biological mass spectrometry.Although rarely used today, FAB was widely used for metabolite analysis throughout the 1980's and into the early 1990's, for example in bile acid and steroid analysis, and protocols developed for metabolite analysis during this era are easily incorporated into analytical procedures using API methods.
FAB is most suitable for the ionisation of polar or ionic biomolecules. FAB ionisation is achieved by the generation of a fast atom beam of neutral atoms (6–8 keV kinetic energy, usually Ar or Xe atoms) in the FAB gun by a process of ionisation, acceleration and neutralisation, which impinges on a viscous solution of sample dissolved in a matrix, usually of glycerol. In the positive-ion mode proton transfer reactions result in the formation of protonated molecules, [M + H]+, while in the negative-ion mode deprotonated molecules are formed, [M - H]-. Usually, both protonated and deprotonated molecules are stable, and little fragmentation occurs in the ion source. LSIMS is very similar to FAB; however, a beam of Cs+ ions (20-30 keV), rather than a beam of neutral atoms, is used to bombard the matrix. LSIMS spectra are essentially identical to those generated by FAB, and in this chapter, for simplicity, both ionisation modes will be referred to as FAB.
Negative-ion FAB was found to be particularly suitable for the ionisation of bile acids and steroid sulphates, alleviating the need for hydrolysis, solvolysis and derivatisation reactions necessary for GC-MS analysis. Urine and plasma samples can be analysed by FAB following a simple C18 solid-phase extraction step, allowing the rapid diagnosis of certain liver diseases, e.g. cerebrotendinous xanthomatosis (CTX), and 7α-hydroxylase deficiency. FAB is a vacuum ionisation method and is not suitable for combination with regular HPLC. However, capillary column HPLC (250 µm i.d., 2µL/min flow-rate) has been successfully interfaced with FAB.
126.96.36.199 Atmospheric Pressure Ionisation (API)
(a) Electrospray (ES). Like FAB, ES is suitable for the analysis of polar and ionic biomolecules. ES occurs at atmospheric pressure and is readily coupled with HPLC. Fenn and colleagues were the first practitioners of ES mass spectrometry and by the turn of the last century, ES had almost completely replaced FAB for the analysis of polar and ionic metabolites.
In ES, the analyte is dissolved in a solvent (very often methanol, ethanol, aqueous methanol or ethanol, a mixture of acetonitrile and water, or of chloroform and alcohol), and sprayed from a metal or fused silica capillary (needle) of 20-100 µm i.d. at a flow rate of 1-500 µL/min (Figures 1.2 and 1.3). An electrospray is achieved by raising the potential on the spray capillary to ~4 kV (+ 4 kV in the positive-ion mode, and -4 kV in the negative-ion mode) and applying a back pressure to the contents of the capillary (e.g. via a syringe pump or HPLC pump). The resulting spray of charged droplets is directed toward a counter electrode which is at a lower electrical potential (Figure 1.2). As the spray of fine droplets travels towards the counter electrode, the droplets lose solvent, shrink and break up into smaller droplets. The small offspring droplets are derived from the surface of their predecessors, which contain the highest concentration of charge, and hence the offspring droplets are generated with an enhanced charge-to-mass ratio. Eventually, the droplets become so small that the charge density on the droplets exceeds the surface tension and gas-phase ions are desorbed (ion evaporation model), or alternatively very small droplets containing a single charged species completely lose solvent leaving the residual charged species free (charge residue model). Surface active compounds tend to be enhanced in the small droplets, and hence are preferentially brought into the gas phase. The counter electrode contains a circular orifice through which ions are transmitted into the vacuum chamber of the mass spectrometer. By traversing differentially pumped regions via skimmer lenses, the ions are transmitted to the high vacuum region of the mass spectrometer for subsequent analysis (Figure 1.2). Early ES experiments were performed at flow rates of 5-50µL/min, compatible with microbore HPLC columns, although today ES interfaces are compatible with 4.2 mm columns operating at 500 µL/min flow rates. It should, however, be remembered that ES is a concentration rather than a mass dependent process, which effectively means that maximum sensitivity is achieved with high concentration, low volume samples, analysed at low flow rates (e.g. 1 pmol/µL, 1 µL, 1 µL/min) rather with dilute, high volume samples, analysed at high flow rates (cf. 0.02 pmol/µL, 50 µL, 50 µL/min). So, theoretically, a combination of low flow rate HPLC with ES should provide better sensitivity than conventional flow HPLC with ES. This has been particularly exploited by the proteomics community, who combine low flow rate HPLC with micro-ES or nano-ES. For a more detailed discussion on the electrospray process the reader is directed to the excellent volume edited by Cole, and series of articles published in the Journal of Mass Spectrometry and Mass Spectrometry Reviews.
Today, the terms "micro-ES" and "nano-ES" are interchangeable, although the terms were originally coined to discriminate between two slightly different forms of ES. The term micro-ES was initially coined by Emmett and Caprioli and used to refer to a miniaturised form of pressure-driven ES (i.e. pumped flow) operated at sub µL/min flow rate. Alternatively, nano-ES was invented by Wilm and Mann and differs from micro-ES in that it is a pure form of ES, where sample flow is at nL/min rates and initiated by the electrical potential between the capillary tip and counter electrode (Figure 1.2), rather than being pressure driven. We prefer the term low flow rate ES to either micro-ES or nano-ES, and use it here to refer to ES operated at flow rates of 1 µL/min and below.
(b) Thermospray (TS). TS mass spectrometry predates ES. TS, like ES, is a technique which involves the spraying of analyte dissolved in solvent from a capillary into an ion source at atmospheric pressure. But TS differs from ES in that vaporisation and ionisattion are a result of thermall heating the spray, as opposed to raising the sprayer to a high potential. TS performs best with buffered aqueous mobile phase (0.1-0.01 M ammonium acetate), and droplets become charged by an uneven distribution of cations and anions between droplets, with the result that gaseous [M + H]+, [M + NH4] or [M + Na]+ ions are formed in the positive-ion mode in an ion evaporation process similar to that occurring in ES (ion evaporation). In the negative-ion mode [M - H]- ions are formed, and this is the ionisation mode of choice for the analysis of acidic metabolites. TS can be operated in two modes: "filament-on" mode or 'filament-off' mode (direct ion evaporation). By the incorporation of a filament in the ion source, analytes which are not readily ionised by direct ion evaporation can be ionised via a chemical ionisation process in the "filament-on" mode. Spectra of biomolecules, e.g. steroids and bile acids, generated by ES and TS are similar; however, dehydration of the protonated or deprotonated molecule tends to occur to a much greater extent in TS than in ES.
(c) Atmospheric Pressure Chemical Ionisation (APCI). APCI is a technique that has become popular for the ionisation of neutral biomolecules. It is very similar to TS described above. Analyte dissolved in solvent is sprayed into an atmospheric pressure ion source. Vaporisation of sample and solvent is achieved by the application of heat, and ionisation of analyte is achieved by a chemical ionisation event. The APCI source differs from the ES source in that it additionally contains a corona discharge needle. Analyte ionisation can be achieved by two processes.
(i) The first is a primary CI process. The nebulised spray results in small droplets of differing charge formed as a result of statistical random sampling of buffer ions (cf. TS). The charged droplets will shrink, as in the ES process, with the eventual formation of gas phase buffer ions, analyte molecules (M) and solvent molecules (Sv). If ammonium acetate [NH4]+ is the buffer gas phase, [NH4] and [CH3CO2]- ions will be generated. The buffer gas ions will be free to react with analyte molecules in a CI event to generate analyte ions (eqns 6 and 7).
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In the positive-ion mode the exact products of the CI event will depend on the gas-phase basicity of the analyte, solvent and buffer. Adduct ions can also be formed (e.g. [M + NH4] + and [M + CH3CN + H]+ in aqueous acetonitrile buffered with ammonium acetate). When the ion source is operated in the negative-ion mode, the products of the CI event will depend on the gas-phase acidity of the sprayed components.
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(ii) APCI can also be achieved in a secondary process, in which electrons from the corona discharge ionise nitrogen gas in the APCI source, leading to the eventual CI of the analyte. In the positive-ion mode, again the eventual products depend on the proton affinity of the components (eqn 8), while in the negative-ion mode the gas phase acidity of the components will define which deprotonated molecules are generated.
Excerpted from Metabolomics, Metabonomics and Metabolite Profiling by William J. Griffiths. Copyright © 2008 The Royal Society of Chemistry. Excerpted by permission of The Royal Society of Chemistry.
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Final council vote tally confirms: Hamm, Baker win
More than half of San Clemente's 41,488 registered voters turned out for Nov. 6 municipal elections that awarded City Council seats to Chris Hamm and incumbent Bob Baker.
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Ok, if you are gun news junkie, like we are, then you know that the web has been all worked up, talking about how New Republic Magazine was duped by a fake Twitter photo of the anointed one, President Obama, skeet shooting.
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