text:
Mashups using the same API (6)
Printed by Moo, powered by Dopplr. Gives you Moo stickers using your city colours and trips from Dopplr.
Moomazon lets you create business cards for a book, using details from Amazon.com.
A simple app that receives an MMS sent to a shortcode and posts that into the MOO card creation service. It then SMS the user back with a easy URL that they can visit to continue to design and order...
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The Most Popular API Pairings May Surprise You
The most popular API, in terms of mashup count, is far and away the Google Maps API, which accounts for 41% of all mashups. But when it comes to the most popular pair of APIs, Flickr and YouTube mashups are the most common. Not to be outdone, Google Maps joins Flickr in a near second place.
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The Stipple API is free, has no request limits as of yet, and OAUTH 2 will be supported in the future. According to its website on the API, it is "inspired by REST". Stipple announces its rare downtime, which is usually used to migrate data, via its twitter account.
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Sparco Products Heavy-duty Punch
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Universal Office Products 12-Sheet Deluxe Two- and Three-Hole Adjustable Punch, 9/32" Holes, Black
$ 5.69 Original Price$ 17.74
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Accentra Compact Three Hole Punch
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Swingline 10-Sheet Precision Pro Desktop Two- and Three-Hole Punch, 9/32" Holes
$ 13.60 Original Price$ 24.90
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Product Information for Carl Brands HC-240 Medium-Duty Hole Punch
Highlights for Carl Brands HC-240 Medium-Duty Hole Punch
- Product Type:Manual Hole Punch
- Number of Punch Heads:2Adjustable
- Punch Size:0.28"
- Paper Punch Capacity:40 Sheet of 20 lb Paper
Marketing Information
Medium-Duty Two-hole Punch drills up to 40 sheets of standard 20 lb. paper. Preset to punch Two-hole pattern for Two-ring binders. Built-in paper guide ensures perfect positioning. Oversized handle allows easy open-handed punching. Hole-punch offers large capacity waste tray and heavy-gauge steel construction.
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Adjustable centering guides make sure that sheets are properly positioned for punching
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Kelly's Reviews > My Life in France
My Life in France
by Julia Child, Alex Prud'Homme
by Julia Child, Alex Prud'Homme
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message 1: by Nicole (new) - rated it 5 stars
Loved this so much. It literally changed my pal Molly's life- and after I reading it I now refuse to apologize for failed kitchen attempts. HA.


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US Secretary of State Hillary Clinton has arrived in Jerusalem for talks with Israeli Prime Minister Benjamin Netanyahu as expectations rose of a ceasefire soon to end a week of fighting around the Gaza Strip.
However, Gaza's rulers, the Palestinian Islamist movement Hamas, revised a statement that a truce would start overnight, saying it was still waiting for an Israeli response to proposals and did not now expect an announcement until late tonight.
An official in the Egyptian government, whose new, Islamist leadership has been playing peace broker in Cairo, had also said a ceasefire could begin today.
But Israeli officials continued to say that discussions were still continuing.
Israel pressed on with its strikes in the coastal enclave on the seventh day of its offensive and Palestinian rockets still flashed across the border as Clinton arrived in Jerusalem. She meet Netanyahu this morning.
One Hamas official had said a truce might start at 9pm local time. But after that moment passed, a senior figure in the movement, Ezzat al-Rishq, told Reuters in Cairo: "The truce is now held up because we are waiting for the Israeli side to respond.
"We ... must wait until tomorrow."
The Jewish state launched the campaign last week with the declared aim of halting the rocketing of its towns from the Palestinian enclave, ruled by the Hamas militant group that does not recognise Israel's right to exist.
Medical officials in Gaza said 27 Palestinians were killed in the latest violence. An Israeli soldier and a civilian died when rockets exploded near the Gaza frontier, police and the army said.
Gaza medical officials say 134 people have died in Israeli strikes, mostly civilians, including 34 children. In all, five Israelis have died, including three civilians killed last week.
Netanyahu said earlier that Israel was open to a long-term deal aimed at ending Palestinian rocket attacks that have plagued its southern region for years.
Khaled Meshaal, the exiled leader of Hamas, said yesterday that Israel must halt its military action and lift its blockade of the Palestinian coastal enclave in exchange for a truce.
Both Netanyahu, favoured to win a January national election, and US President Barack Obama have said they want a diplomatic solution, rather than a possible Israeli ground operation in the densely populated territory, home to 1.7 million Palestinians.
Israel's military has targeted more than 130 sites in Gaza, including ammunition stores and the Gaza headquarters of the National Islamic Bank. Israeli police said more than 150 rockets were fired from Gaza by the evening.
"No country would tolerate rocket attacks against its cities and against its civilians. Israel cannot tolerate such attacks," Netanyahu said with UN Secretary-General Ban Ki-moon, who arrived in Jerusalem from talks in Cairo, at his side.
"If a long-term solution can be put in place through diplomatic means, then Israel would be a willing partner to such a solution," he said. "But if stronger military action proves necessary to stop the constant barrage of rockets, Israel will do what is necessary to defend our people."
After nightfall, Israel stepped up its Gaza bombardment. Artillery shells and missiles fired from naval gunboats slammed into the territory and air strikes came at a frequency of about one every 10 minutes.
In an attack claimed in Gaza by Hamas's armed wing, a longer-range rocket targeted Jerusalem overnight for the second time since Israel launched the air offensive.
The rocket, which fell harmlessly in the occupied West Bank, triggered warning sirens in the holy city about the time Ban arrived for truce discussions. Another rocket damaged an apartment building in Rishon Lezion, near Tel Aviv.
Rockets fired at the two big cities over the past week were the first to reach them in decades, a sign of what Israel says is an increasing threat from Gaza militants.
In the Gaza Strip, Hamas executed six alleged collaborators, whom a security source quoted by the Hamas Aqsa radio said "were caught red-handed" with "filming equipment to take footage of positions". The radio said they were shot.
Militants on a motorcycle dragged the body of one of the men through the streets.
Along Israel's sandy, fenced-off border with the Gaza Strip, tanks, artillery and infantry massed in field encampments awaiting any orders to go in. Some 45,000 reserve troops have been called up since the offensive was launched.
A delegation of nine Arab ministers, led by the Egyptian foreign minister, visited Gaza in a further signal of heightened Arab solidarity with the Palestinians.
Egypt has been a key player in efforts to end the most serious fighting between Israel and Palestinian militants since a three-week Israeli invasion of the enclave in the winter of 2008-9.
Egypt has a 1979 peace treaty with Israel seen by the West as the cornerstone of Middle East peace, but that has been tested as never before by the removal of US ally Hosni Mubarak as president last year in the Arab Spring uprisings.
Mohamed Mursi, elected Egyptian president this year, is a veteran of the Muslim Brotherhood, spiritual mentors of Hamas, but says he is committed to Egypt's treaty with Israel.
Mursi has warned Netanyahu of serious consequences from an invasion of the kind that killed more than 1,400 people in Gaza four years ago. But he has been careful so far not to alienate Israel, or Washington, a major aid donor to Egypt.
- Reuters
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Derek Lewis
Pre-Owned Car Sales Representative
Koons Baltimore Ford
6970 Security Boulevard
Baltimore, MD 21244
Derek started in auto industry in 2001, all with The Great Organization at Koons. Derek says, "Car Sales is second to breathing to me." He is Master Certified with several awards and honors that mirrors his dedication. He has a beautiful family with his wife of 15 years and two children and a aussie named "Quincy." His hobbies include building model cars, reading and watching old action movies. He also assists on his son's AAU Basketball team with plays and strength training.Read more
17 Reviews of Derek Lewis
June 14, 2016
June 07, 2016
June 03, 2016
"Wonderful friendly experience!"- Richnbaltimore
I usually keep my cars between five and seven years. My last three cars I purchased at Carmax. However, due to an unsatisfactory Non-welcoming a local location, I went to Koons Ford of Baltimore from a coworker's recommendation. I researched the car online, and the next day received an email. I went to the store the following day, and was greeted by my car waiting near the show room . That was awesome! Derek Lewis was my salesman. I absolutely loved the car, and needed the weekend to think about it. No pressure from Koons. I went back two days later, and purchased the car. Everyone has been wonderful, from Derek, the financial advisor, down to the service center employees. I have never experienced this genuine outstanding customer service from an auto dealership. Highly recommend this store!Read More
April 12, 2016
"Best experience of purchasing a vehicle "- Tina Lott
We purchased a Ford Mustang from Derek at Koons Ford Baltimore and he was the nicest, hardest car dealer we have ever worked with. The team of financial people were wonderful. We will never go anywhere else to purchase a vehicle again.Read More
Other Employees Tagged: Tom Snyder
March 09, 2016
"Professional "- sturg
Derek was my salesman was very professional and attentive to my needs I am a small business owner and was in need of a dual purpose vehicle work/pleasure as I have to little ones . In a matter of days he called me with the sweetest Truck I have seen in a long time with super low miles at a great price . Then also had a very pleasant experience with James Moore also got me a great loan at a reasonable interest rate . JUST WANT TO GIVE KOONS FORD A HUGE SHOUT OUT FOR OUTSTANDING SERVICE AND IMPECCABLE CUSTOMER RELATIONS THANK YOU FOR A PLEASANT CAR BUYING EXPERIENCE. John W SturgillRead More
Other Employees Tagged: JAMES MOORE
February 27, 2016
"They were up for the Challenge"- What the Truck
I was looking to replace my 2002 Ford F-350 Dually. It was a Great truck, but for me it was overkill. I was looking for a 2010 F-150 with a long bed. I searched the web and saw many and drove many. All wanted a price way higher than I wanted to pay. The truck is going to be used to pull my motorcycle trailer and not driven every day. I can't take credit for finding Koons in Baltimore. That was my wife's find. She saw it and said it was a Great looking truck and priced well. I called and spoke to Derrick Lewis who from start to finish was nothing short of Great. I made a appointment to drive over from Reston, Va and meet wit him to see the truck. We all love it when a person has the answer and doesn't have to go find it. He was courteous his knowledge was great and he delivered as promised. I told him I'm sold on the truck but I'm married and in our house when buying cars it is a WE thing not a ME thing so he would have to convince us both. Well Derrick, Ray & Steve in finance were up for the challenge and now I have a Great Truck. I'm starting to like it more than my other car (Audi Q7 TDI w/euro sport pkge) I would buy from them again easily. Love that place with it's very cool owner Who fed us on the day we were there.Read More
Other Employees Tagged: Dennis Koulatsos, Ray Baldwin, Steve Ageloff
February 27, 2016
"Friendly, courteous, professionals"- Friendly, courteous, professionals!
My wife and I both agree we had a wonderful sales experience. We initially met with Derek Lewis who stood in the rain while holding an umbrella over my wife and I so that we would not get wet. Who does that these days? He was very knowledgeable and kept us in the loop through the whole process of service that our new "used" car was going through. We worked with Carol Walker for the financing portion of our experience and it was as if we were meeting with an old friend. She was very engaging and personable while maintaining professionalism. I will definitely recommend Koons Baltimore Ford to my family and friends and look there first for my future vehicle needs.Read More
Other Employees Tagged: Carol Walker
December 09, 2015
October 31, 2015
"Always awesome!"- Sivleone
We just purchased our second vehicle from Koons Ford and the experience just as the first time was nothing but enjoyable. The staff is great and easy to deal with. They are attentive listen to your wants and needs and work with you in every aspect of the sale process. Couldn't ask for a better all around dealership experience.Read More
Other Employees Tagged: Carol Walker
June 01, 2015
"Koons Ford WOWED ME!!! "- Kayden
I wanted to say I had the BEST experience at Koons Ford of Baltimore because of my salesman Derek Lewis was soooooo FANTASTIC!! He assisted me to find the perfect truck and on top of me finding my perfect truck I wasn't there long at all. If you go to Koons Ford of Baltimore ask for Derek Lewis because he knows Ford! Derek demonstrated EXCELLENT customer service!!! On top of his busy schedule Derek followed up with emails to make sure when I came to the dealership that they had the truck that I wanted. I also felt like Koons of Baltimore found and offered me the best deal! Thank you Koons & Derek!!!!Read More
Dealership Inventory (1097 Vehicles)
2015 Ford C-MAX Energi
2016 Ford F-350
2016 Ford F-350
2016 Ford F-250
2016 Ford F-250
2015 Ford Flex
2015 Ford C-Max Hybrid
2016 Ford Transit Connect


text:
|Publication number
|Publication type
|Application number
|Publication date
|Jul 20, 2004
|Filing date
|Nov 1, 2002
|Priority date
|Nov 16, 2001
|Fee status
|Also published as
|Publication number
|John Gerard Whateley
|Original Assignee
|Amersham Biosciences Uk Ltd.
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (1), Referenced by (3), Classifications (8), Legal Events (5)
|External Links: USPTO, USPTO Assignment, Espacenet
This invention relates to an apparatus and a method for the measurement of the heat generated in a chemical or biochemical reaction, by detecting and measuring a change in the conformation of a polymer transducer responsive to a heat change and being bound to the surface of a waveguide of an interferometer. The conformational change is detected by optical means and is compared with a control.
Assays involving biochemical reactions have hitherto generally required the use of a labelled reagent, for example a radioactive label or a fluorescent probe. Fluorescent labels have the disadvantage that they are generally bulky molecules that can change the structure and biological activity of the biochemical reagent that they are used to label. Radioactively labelling a molecule has the advantage that the molecule usually remains in its "native" conformation. However, there are other drawbacks to this method, including: i) the radioactive decomposition of the molecule; ii) the radioactive label can generally only be inserted synthetically into the molecule; iii) if the radioactive label is added as part of a labelling reagent, it has the same disadvantage as fluorescent probes; and iv) handling and disposal of radioactive material. In those cases where no labelled reagent is used, it is usually necessary to develop methods for the separation of the reactants from products, prior to analysis of the amounts of reactant consumed, or product formed.
Ideally, a method that would use "native" reagents and could monitor the progress of biochemical processes would be more suitable for the identification of enzyme inhibitors, antagonists, etc.
Essentially all biochemical and chemical reactions are associated with a change in enthalpy, and either take up or give off heat to the environment during the reaction. Since these reactions are typically performed in aqueous or organic solvent, the solvent changes temperature during the reaction. If this temperature change can be measured, then the rate and extent of the reaction can be measured directly. The ability to measure enthalpy changes through temperature changes in the solvent would obviate the need for labelling reagents, or the isolation of substrates or products to measure the extent of the reaction.
Microcalorimeters are useful for analytical measurements of biological or chemical reactions but are not currently useful in high throughput screening (HTS) applications for pharmaceutical screening purposes. Currently, microcalorimeters are capable of measuring enthalpy changes of 1 μJ or greater and usually require test volumes of 1-1.5 ml. Most biochemical reactions have enthalpy changes of the order of magnitude ˜100 kJ/mol. In a typical biochemical assay volume of 100 μl and a substrate concentration of the order of 1 nanomolar, the maximum amount of heat released by a sample and the corresponding temperature increase (assuming no heat is lost to the environment), is of the order 10−8� K. Thus, the temperature change associated with such assays is below the detection limits of current microcalorimeters.
Integrated optical Mach-Zehnder interferometers (IO-MZI) have been employed as sensors for the detection and measurement of biological and biochemical interactions.
For example, Brosinger et al (Sensors and Actuators, (1997), B 44, 350-355) describe an IO-MZI for use as an affinity sensor in which one branch of the device was coated with antigen and a phase change caused by specific interactions of immunoglobulins with the antigenic surface.
The present invention relates to an improved method and an apparatus for the measurement of heat changes in a chemical or biochemical reaction that increases the sensitivity of measurement compared with conventional methods, while concurrently reducing assay volumes and the quantity of reagents compared with those conventionally used in such assays. In addition, as discussed by Brosinger et al (loc cit), a major disadvantage of affinity biosensors is the difficulty in distinguishing unwanted non-specific reactions of protein-containing solutions with the sensor surface. The present invention overcomes this problem by preventing the sensor surface making contact with the sample.
In a first aspect of the invention there is provided a method for measuring a heat change in a reaction to be studied, the method comprising:
i) contacting a surface of a waveguide with a liquid including one or more components of said reaction to be studied said surface having a transducer responsive to a heat change in said reaction and being coated on and bound thereto;
ii) directing a beam of electromagnetic radiation through said waveguide in the absence and in the presence of an initiator of said reaction to produce a fringe pattern including a plurality of spaced light bands whose positions shift in response to changes in the heat of said reaction;
wherein the positional shifts of said light bands in said fringe pattern measured in the absence and in the presence of said initiator are used to calculate the heat change in said reaction.
In a second aspect of the invention, there is provided a method for measuring a heat change in a reaction to be studied, the method comprising:
i) contacting at least a portion of the surface of a sample waveguide and of a reference waveguide of an interferometer with a liquid including one or more components of said reaction, each said surface having a transducer responsive to a heat change in said reaction and being coated on and bound thereto;
ii) directing a beam of electromagnetic radiation simultaneously through said sample waveguide and said reference waveguide such that a fringe pattern is produced said fringe pattern including a plurality of spaced light bands whose positions shift in response to changes in the heat of said reaction;
iii) adding to said one or more components in contact with said sample waveguide an initiator of said reaction; and
iv) determining the heat change in said reaction by measuring the positional shifts of said light bands in said fringe pattern.
In a third aspect of the invention, there is provided an interferometric system, said system comprising a sample waveguide, a reference waveguide, and an outgoing waveguide;
wherein said sample and reference waveguides are formed of a material exhibiting an index of refraction, said sample and reference waveguides being joined at a first junction to an inlet waveguide and at a second junction to the outgoing waveguide such that electromagnetic radiation is allowed to pass simultaneously in parallel through said sample and reference waveguides;
characterised in that each of said sample waveguide and said reference waveguide comprises a surface that is coated with a transducer responsive to a heat change in a reaction and is bound thereto.
In a fourth aspect of the invention, there is provided use of an organic polymer or a biological macromolecule as a transducer for the measurement of a heat change generated in a chemical or a biochemical reaction to be studied.
The present invention therefore relates to the indirect determination of the heat of a reaction to be studied, suitably a biological or biochemical reaction, by monitoring a change in the conformation of molecules of a transducer that is in close proximity with a liquid reaction mixture, the transducer being coated onto and bound to the surface of the sample and reference waveguides of an interferometer. The change in conformation of the transducer molecules results in a change in the refractive index of the transducer, thereby producing a phase change in the electromagnetic radiation being transmitted through the waveguide.
Suitably, the interferometer is a Mach-Zehnder interferometer.
Suitably, the transducer is a material that is responsive to a heat change in the sample of the reaction to be studied, when the sample is in close proximity, or is in contact with, the transducer. The transducer is suitably a polymer, including an organic polymer or a biological macromolecule, which may be coated onto the surface of a region (the sensor region) of the sample and the reference waveguides. The polymeric transducer should be inert, that is, it must not interact chemically with the reagents taking part in the chemical or biochemical reaction under study.
Suitable polymers for use in the method according to the present invention include oligo- and polypeptides of greater than 10 amino acid monomer units, proteins, nucleic acids, oligo- and polynucleotides of greater than 10 nucleotide monomer units, and poly-olefinic compounds.
Preferred polymers for use in the method of the invention may be selected from the group consisting of: trypsin, myoglobin, ribonuclease A, fibrin, poly-L-glutamate, poly-α-benzyl-L-glutamate, salmon DNA, sea urchin DNA, polyadenylic acid (double-stranded), poly adenylic acid (single-stranded), polyadenylic acid-polyuridylic acid, poly-methacrylic acid, poly-methyl methacrylate and poly-allylamine.
Suitably, the optical waveguides are formed of a material that exhibits a refractive index to the electromagnetic radiation that will pass through the waveguide. The design and fabrication of a suitable optical waveguide will be well known to the skilled person. See, for example, Heideman and Lambeck, Proc. IEEE/LEOS Symp. (1997), pp 29-32). Suitable materials for forming the waveguide are disclosed in U.S. Pat. No. 5,465,151. Suitable polymeric materials for forming the optical waveguide are disclosed in WO 01/06240 (Gharavi, A.), for example, polyimide-alkoxy sulphone diazo stilbene, polyimide-dialkylamino nitro azo and polyimide-dialkyl amino diazo. Examples of other materials that may be used for fabricating optical waveguides include, but are not limited to, silicon oxide, silicon nitride, gallium arsenide, as well as glass and other siliceous materials. Particularly preferred materials are selected from silicon oxide and silicon nitride.
The electromagnetic radiation that is transmitted through the waveguide is suitably light radiation in the visible to near-infra-red region, preferably in the visible region from 400 nm to 1000 nm. The visible light is suitably supplied by a laser or a laser diode source, for example a VCSEL at 850 nm or helium neon laser at 632.8 nm.
Suitably, the said one or more components of said reaction are components of a chemical or biochemical reaction. In principle, any chemical or biochemical reaction may be studied using the apparatus and method according to the present invention. Examples of reactions that may be studied include, but are not limited to, enzyme assays, immunoassays, hydridization assays (for example DNA/DNA, RNA/RNA, RNA/DNA), protein/nucleic acid interactions and protein/protein binding assays. The apparatus and method is suitable for the measurement of the heat generated or taken up in any chemical reaction (in which ΔH≠0), for example catalytic conversions, adduct addition and cellular biochemical processes in which changes in the metabolic status of an organism may be accompanied by changes in the heat output from the organism.
In order to clarify the principle and the function of the invention, reference is now made to the accompanying drawings in which:
FIG. 1 shows a plan view of the optical system according to the invention and is based on a Mach-Zehnder interferometer;
FIG. 2 is a schematic diagram showing, in cross-section, the sensor region of the apparatus according to the present invention;
FIG. 3 is a schematic diagram showing, in cross section, a sample chamber for receiving a sample of a chemical or a biochemical reaction to be studied;
FIG. 4 is a schematic diagram illustrating, in cross section, an alternative sample chamber whereby channels or depressions are formed in the transducer layer to contain samples of reactants;
FIG. 5 is a schematic diagram showing, in cross section, a sample chamber provided with a cover or "lid" so as to insulate the chamber;
FIG. 6 is a schematic diagram shown in cross section, whereby the sensor surface is contained within a flow cell.
Referring to FIG. 1, a Mach-Zehnder interferometer (10) is shown and is constructed having an inlet waveguide (11), an outgoing waveguide (14), a first channel (12) comprising a sample waveguide (15) and a second channel (13) comprising a reference waveguide (16). The two channels (12, 13) are joined in parallel at a first junction (17) to one end of the inlet wave-guide (11), and at a second junction (18) to one end of the outgoing waveguide (14). A beam (19) of electromagnetic radiation enters the inlet waveguide (11) and is guided down the inlet waveguide toward the first junction (17). At the first junction (17), the waveguide splits the beam (19) into both channels so as to allow a reference portion of the beam (19 ref) to be transmitted into the reference waveguide (16) and a sample portion (19 samp) to be transmitted into the sample waveguide (15). After passing through the reference and sample waveguides, the beams (19 ref, 19 samp) are reunited at the second junction (18) and exit through the outgoing waveguide (14). Any difference in the interference patterns between the first channel and the second channel will generate interference in the outgoing waveguide (14) that will be detected as a change in the transmitted intensity of the electromagnetic radiation.
The external surface of the sample waveguide and the reference waveguide are each suitably equipped with a sensor region which comprises a coating of a transducer material that is responsive to changes in heat in the reaction (either exothermic or endothermic) under study. Suitably, the transducer is a polymer layer, preferably an organic polymer or a biological macromolecule. It is known that biological macromolecules undergo a conformation change when they are subjected to a temperature change. Such conformational changes are accompanied by corresponding changes in refractive index. When the polymer is formed as a layer bound to the external surface of the sample waveguide and the reference waveguide, any change in the refractive index of the polymer will affect the refractive index of the sample and reference waveguide, thereby causing a change in the interference fringe pattern produced as a result.
FIG. 2 is a schematic diagram showing, in cross-section, the sensor region (20) of the apparatus according to the present invention. An optical waveguide system is known. See for example, the system described in WO 98/58285 (Heideman et al), which comprises a light guiding layer supported by a carrier support (21), preferably composed of SiO2 having a thickness of about 500 nm. The light guiding layer comprises layers of Si3N4 (passive light guiding layer (22) and having a thickness, for example, of about 20 nm) and ZnO (electro-optical light guiding layer (23) having a thickness of about 500 nm). A transducer layer (24) is attached to the light guiding layer.
Suitably, the transducer polymer will be coated as a layer to a depth of between 50 nm and 250 nm, preferably about 100 nm. The polymer will be coated as a layer on both the sample and reference waveguides of the MZI.
A number of methods have been described to couple polymers to silicon surfaces. Generally they may be divided into methods which involve passive absorption or covalent linkage. Thus, the polymer may be dissolved in an organic solvent and the required volume applied to the surface of the sensor. The solvent is then driven off either passively or actively and the resulting polymer layer is left on the sensor surface. Alternatively, covalent coupling methods can be used to couple the polymer to the surface of the sensor. For example, Halliwell, C. M. and Cass, A. E. G., Analytical Chemistry, (2001), 73(11), 2476-83) describe the coupling of nucleic acids to surfaces using (3-mercaptopropyl)-trimethoxysilane. The silanization of surfaces has been extensively reviewed in the literature, for example, Kallury et al, Analytical Chemistry, (1988), 60, 169-172; Leyden in Silanes, Surfaces and Interfaces, Gordon and Breach, New York, 1986.
In practice, the surface of the sensor is cleaned using, for example, pirhana acid or oxygen plasma. The surface may then be silanised by treatment with 0.1% 3-aminopropyl triethoxysilane in toluene for approximately 1 hour, followed by heating at 110� C. for 2 hours. Following this treatment, the amino-functionalised surface is activated by 1% cyanuric chloride and 1% tribenzylamine for 4 hours at room temperature. The surface is then washed with toluene and petroleum ether, and then dried. Solutions of polymer in aqueous buffers such as PBS can now be added and incubated for 1 hour at room temperature. The surface is washed with 0.01% Tween to remove unbound material, and then with water.
The sample may be brought into contact with the sensor region in various ways as shown schematically in FIGS. 3 to 6.
FIG. 3 shows a sample chamber for receiving a fluid sample of a chemical or a biochemical reaction to be studied. The sample chamber may be held in place over the sensor surface by mechanical means such as a clamp, or alternatively the sample well may be permanently attached to the sensor surface using adhesive. Suitably, the sample well (25) is of a one-piece moulded construction and may be formed from rigid materials, which are resistant to the aqueous, and/or aqueous/organic media used in the chemical or biochemical reactions under study. Suitable materials are selected from rigid plastic or polymeric materials. Alternatively, the reservoir may be formed by machining methods in the surface of a plastic or polymeric block. Preferred plastics are selected from polystyrene, polycarbonate, polypropylene, or poly-tetrafluoroethylene (PTFE; Teflon™).
An alternative approach is to form the sample chamber into the surface of the transducer polymer layer, as shown in FIG. 4, as depressions (26) (or channels) to which samples of the chemical or biochemical reaction may be added directly.
Suitably, the sample chamber (25) and the depressions (26) for holding the reaction components may be of any cross-sectional shape, such as square, rectangular, circular, trapezoid and triangular and are sized to give a floor area between 100 μm2 and 10,000 μm2, preferably between 500 μm2 and 1,000 μm2. The plastic or polymer surface of the sample chamber may be selectively treated or modified to permit cell attachment and/or growth for the study of cellular reactions. Treatment preferably involves exposure to intense UV light to modify the polymer surface or alternatively the use of high voltage plasma discharge using known techniques (see Amstein, C. F. and Hartmann, P. A., J. Clin. Microbiol., (1975), 2(1), 46-54) to create a negatively charged surface suitable for cell growth and attachment. Cell attachment may be further improved by the application of additional coatings to the surface of the cell growth chamber, eg. polylysine, collagen, fibronectin.
In a further embodiment, as shown in FIG. 5, the sample chamber (25) is provided with a cover or "lid" (27) so as to insulate the sensor surface from the environment, thereby minimising any loss of heat from the reaction under study. Samples may be introduced into the sample chamber and applied directly to the surface of the transducer polymer layer by injection, for example, by means of a syringe (28) through the lid (27). Furthermore, the lid (27) may be used to control the environment of the chamber by the passage of a coolant or heating fluid, such as water, through tubing (not shown) attached to a hollow lid.
In a still further embodiment, the sensor surface is contained within a flow cell as shown in FIG. 6. A liquid sample containing components of the reaction to be studied enters the sample chamber by means of a reagent inlet pipe (29 a) set into one side of the sample chamber. The sample leaves the sample chamber via an exit pipe (29 b) set into the opposite side of the chamber. In use, reagents or wash solutions may be added to the sample chamber by pumping, for example, by means of a peristaltic pump or vacuum pump.
To perform a measurement of a change in the heat generated in a reaction, the components of the chemical or biochemical reaction to be studied (the reactants), typically in a reaction volume of 50-90 μl, are placed in contact with the sensor surface of the sample waveguide and the reference waveguide, without the initiator of the reaction being present. Laser light is allowed to pass through the waveguides and the fringe pattern, (ie. a series of spaced light bands), is recorded. The reactants are allowed to achieve thermal equilibrium, as judged by no change in the fringe pattern from the MZI. At this time, an initiator is added to the reactants in contact with the sample waveguide in a typical volume of 1-10 μl and in a time of less than 0.1 sec. Any changes in the position of light bands of the fringe pattern are recorded continuously and the data stored by means of a computer.
For example, the enzyme β-lactamase may be assayed using the apparatus of FIG. 3 as follows:
i) prepare a 1 mM solution of penicillin G in PBS pH 7.0;
ii) add 50 μl of the 1 mM solution of Penicillin G to the sample wells located on both the sample and reference waveguides of the MZI;
iii) prepare a solution of the enzyme β-lactamase at 50 IU/10 μl in PBS pH 7.0;
iv) place 10 μl of the enzyme β-lactamase at 50 IU/10 μl in PBS pH 7.0 in the initiator addition device, such as a syringe;
v) record the fringe pattern from the MZI until a stable reading is obtained, continue to record this reading for 1 minute;
vi) add the enzyme to the penicillin solution in the sample well and simultaneously add 10 ul of buffer to the reference well in less than 0.1 seconds;
vii) record the fringe pattern for a period of 10 minutes;
viii) determine the rate of change of the fringe as fringes/second;
ix) repeat steps i) to viii) using a range of concentrations of Penicillin G;
x) using the data from step viii), plot the rate of change of the fringes (as fringes per second) versus the concentration penicillin G to obtain data for the kinetic properties of the enzyme such as Vmax and Km without the need for absolute knowledge of the temperature or ΔG involved in the reaction.
The correlation of changes in the fringe pattern of the MZI to energy changes in the chemical or biochemical reaction may be accomplished in a number of ways.
1. A platinum resistor may be placed within the sample chamber in the presence of a suitable quantity of water. Electric current may be passed through the resistor so that the amount of energy dissipated may be readily calculated. The resultant changes in the position of light bands in the fringe pattern may then be related to the energy passed through the resistor and the energy so received. This process may be repeated for a number of different current settings.
2. In an alternative and preferred method, the energy released from a chemical or biochemical reaction may be used. For example, Wadso, (Thermochemica Acta, (2000), 347, 73-77) describes the use of a number of chemical reactions which generate well-defined amounts of heat. This could include the aqueous dissolution of propan-1-ol.
Advantages of the Present Invention
i) The method of the invention may be used with sample volumes of 100 μl or less.
ii) The surface of the transducer polymer layer is non-functionalised and the method according to the present invention does not rely on specific binding of reactants to the surface of the waveguide. The apparatus may therefore be used for the measurement of heat generated with different reaction types.
iii) Many prior art calorimeters use direct detection of heat change, which is typically measured via a change in the signal from a metal or semi-conductor device and is related to the Seebeck coefficient of the material being used. The Seebeck coefficient of materials limits the sensitivity of such devices to about 10−4 to 10−5� C. and therefore requires relatively large sample volumes, typically greater than 1 ml. The present invention utilises an indirect optical detection system which is not limited by the Seebeck coefficient. The sensitivity will be of the order of 10−6 degrees change in temperature.
iv) The apparatus and method according to the invention may be used in real time measurements of heats of chemical or biochemical reactions. When a difference appears between the sample and reference arms of the MZI, this difference will be reported instantaneously.
It is apparent that many modifications and variations of the invention as hereinabove set forth may be made without departing from the spirit and scope thereof. The specific embodiments described are given by way of example only, and the invention is limited only by the terms of the appended claims.
|Cited Patent
|Filing date
|Publication date
|Apr 21, 1994
|Nov 26, 1996
|The Royal Institution For The Advancement Of Learning
|Homogeneous immunoassay system employing fourier transform infrared spectroscopy
|Citing Patent
|Filing date
|Publication date
|Oct 23, 2004
|Jul 24, 2007
|Gultekin David H
|Measurement of thermal diffusivity, thermal conductivity, specific heat, specific absorption rate, thermal power, heat transfer coefficient, heat of reaction and membrane permeability by nuclear magnetic resonance
|Sep 28, 2010
|Affymetrix, Inc.
|System, method, and product for generating patterned illumination
|Jul 13, 2007
|Jan 24, 2008
|Affymetrix, Inc.
|System, method, and product for generating patterned illumination
|U.S. Classification
|385/12, 436/524, 385/147, 250/227.14
|International Classification
|Cooperative Classification
|European Classification
|Dec 2, 2002
Owner name: AMERSHAM BIOSCIENCES UK LIMITED, GREAT BRITAIN
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Free format text: CHANGE OF NAME;ASSIGNORS:BATESON;AMERSHAM LIFE SCIENCE;AMERSHAM LIFE SCIENCE LIMITED;AND OTHERS;SIGNING DATES FROM 19970528 TO 20020124;REEL/FRAME:017325/0248
Owner name: GE HEALTHCARE UK LIMITED, UNITED KINGDOM
Free format text: CHANGE OF NAME;ASSIGNORS:BATESON;AMERSHAM LIFE SCIENCE;AMERSHAM LIFE SCIENCE LIMITED;AND OTHERS;SIGNING DATES FROM 19970528 TO 20020124;REEL/FRAME:017325/0248
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Mar 13, 2013
Starchild1952 Profile
Forums Owned
Recent Posts
Race in America: Why are blacks being seen as racists?
Q Where do you think you white people come from? Think again and listen to the DNA of your body. Ask the Berbers of Africa where you come from, blues and all. You are from the Ghetto, that will explain your attitude. See how smart you are. (Nov 26, 2013 | post #9556)
Race in America: Why are blacks being seen as racists?
I have been call all kinds of names by all kinds of people.I tell'em "get in line." To make these problems with our country go-away will take the effort of the good in us all. (Nov 22, 2013 | post #9535)
Race in America: Why are blacks being seen as racists?
The truth,45 million blacks,300 million white,here in America.And for pimping .Well, they were white slave owner. And go to Las Vegas and meet their decendents.I am ex-fire fighter who have saved many racist people, black and white,but I don't wear my hat backward like these kids on TV. It will take good people out here to try to put and end to the senseless killing.These John W Gacy are every where. (Nov 21, 2013 | post #9520)
Race in America: Why are blacks being seen as racists?
What you must understand the race card was invented by our government.This was a way to make fool out of us all and have us divided.So we can be controled and I must say it has worked.How many of us kill our own kind and think nothing of it.How many of us black and white look up in the sky for God. Take a closer look at history my friends instead of your community. How many blacks speak their native tongue,and yet claim you know the truth.Black and White American don't know the real truth.You know what the government tell you.We have more prisons than any other country in the world.I wonder why? (Nov 20, 2013 | post #9515)
Whoops! Police Chief Decides Vigilantes like Zimmerman Ca...
Was John W Gacy a racist? How many times you shot him? That white man name Jesus really something. (Nov 18, 2013 | post #25)
The 25 Most Dangerous Cities in the U.S. Are Mostly Nice ...
From my experience in living in America, poor people love to blame other for their own action,no matter where they live.Infact they have more children then the rich.I saw a woman with 16 children on TV like she was a movie star and never been married a day in her life. You are responsible for your own action in life not other. (Nov 18, 2013 | post #20227)
What about the Mob in Detroit,Philly,Chi cago,and New York? Would you run your mouth-off at those good White folk? I know, I know, that White man name Jesus Love Ya! (May 12, 2013 | post #76)
I was born here in America.But I don't think of my self as Afro American That is a Greek- Roman term. Africa is a Euro term. I am from the blood line of the children of Cush. The first time I made Love to a woman was in England.She was a beautiful white woman. She did not really like black men.She just like me.I also had girlfriends in other countries including America. I plan to visit Norway. (Mar 13, 2013 | post #72)
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SUMMARY: This Case Study includes some useful no-nonsense tips on business-to-business marketing on a tight budget. Find out how software marketer Christine Smith saved money on seminar road shows; got influential analysts at firms such as Gartner to like her Company (hint: Use fewer than 10 slides); and doubled the number of white paper downloads from her Web site.
If you are trying to generate more sales leads for your company, we hope you get at least one great "stealable" idea from this.
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The Effortless Experience: Conquering the New Battleground for Customer Loyalty by Matthew Dixon, Nick Toman and Rick DeLisi
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text:
What It's Like To Get Eaten By A Grizzly Bear
Brad Josephs was capturing footage for BBC'S "Great Bear Stakeout" when one of the younger bears ate the GoPro camera. We are left with an unharmed camera, an unharmed bear and some awesome footage.
2. Let's watch that again.
3. And now here he is frolicking afterwards.
4. But don't forget he can do this:
- The men who attacked Istanbul's main airport on Tuesday, killing 44 people, were from Russia, Uzbekistan, and Kyrgyzstan.
- During the 2014 Scottish referendum, Trump said independence would be "devastating" for Scotland. Last week he celebrated Brexit.
- A federal judge halted Mississippi's anti-LGBT religious exemption law moments before it was set to go into effect.


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Cox Report
Only Available in Archive Formats.
This week the Cox report arrived on Capitol Hill, accusing the Chinese of old-fashioned spywork. News of the Cox investigation triggered a tightening of export regulations two months ago. Satellite builders say the new restrictions are crippling the $66 billion industry. NPR's David Kestenbaum reports.
Please keep your community civil. All comments must follow the NPR.org Community rules and terms of use, and will be moderated prior to posting. NPR reserves the right to use the comments we receive, in whole or in part, and to use the commenter's name and location, in any medium. See also the Terms of Use, Privacy Policy and Community FAQ.


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Posted in Press Releases on 08 July 2014
Yorkshire's Post War Glamour Girls have built up a huge reputation over the past year following the release of their acclaimed and outstanding album 'Pink Fur'. Now the four-piece are set to swiftly return with a brand new single 'Gustave / Lolong', released through the Too Pure label on September 1st.
Reminiscent of The Birthday Party with vocals evocative of Nick Cave crossed with Frank Black (if they both hailed from Leeds), the four-piece have spent the past two years building up an impressive body of work, showcasing an innate knack for creating intricately layered and intelligent compositions with an almost theatrical Arcade Fire-like build up.
And while the mainstream has noticed, with the band already receiving heavy support from Clash, DIY, The 405, The Line Of Best Fit, and being invited to perform on the NME/BBC Radio 1 stages at the Reading and Leeds festivals, it's the incredible amount of underground fan-driven support that has marked Post War Glamour Girls out as a major band to watch.
Last year also saw the band perform a series of shows at the likes of Live at Leeds and Long Division, tour supports with both PINS and I Like Trains, as well as performing to over 800 people at Leeds University Stylus.
The four-piece released their expansive and epic ten-track debut album, 'Pink Fur', this February. Recorded in break-neck speed with Lee Smith and Jamie Lockhart (The Cribs, Forward Russia) at Greenmount Studios, the new album has been winning widespread praise since its release.
Post War Glamour Girls continue their rise with a new single 'Gustave / Lolong', released through Too Pure on limited edition crocodile green vinyl on September 1st 2014.
25 July - SHEFFIELD - Tramlines Festival
26 July - TOPCLIFFE - Deershed Festival
08 Aug - SKIPTON - Beacons Festival
13 Aug - WAKEFIELD - Long Division Festival
The House actor has been entertaining audiences almost four decades
Ahead of 'Fantastic Beasts And Where To Find Them' JK Rowling has revealed the history of Ilvermorny, the 'American Hogwarts'.
The potential casting of DiCaprio has been called another example of Hollywood's 'whitewashing'.
Kanye West is causing friction among his peers again.
'Game of Thrones' star Kit Harington has revealed that got into a fight with a much bigger...


text:
I thought we might gather some useful information about the failure rates for Nvidia GPUs after all of the recent brouhaha over the known problems in Nvidia mobile GPUs and rumored problems with some of their other products by posting a poll and asking for comments from folks affected by the problem. However, it turns out that we didn't learn much that we didn't know.
The poll results, such as they are, indicate incredibly high failure rates for all of the Nvidia parts in question. Trouble is, I also asked people who voted about a failure to "please follow up by posting a comment explaining what failed on you and how." To date, we have 3156 votes, 438 of which indicate one or more GPU failures, and I count two posts that might be relevant to the problem: one from Hatting about a failed "8600 GTX" (wait, what?) and one from adisor19 about two failed Dell XPS 1330 laptops. Neither commenter provided any information about the nature of the failure.
That makes me think we've pretty conclusively proven that fanboys and corporate drones will indeed stuff the ballot box in an online poll (like I said, nothing we didn't know), but I'm in no way convinced we're seeing a phenomenon on the order of the GXP fiasco. Nvidia has already admitted the problem with laptop chips is a real one, and we have indeed seen some anecdotal evidence about that, but nothing we've seen here or elsewhere convinces me that desktop G84, G86, G92, or G94 chips are failing at abnormally high rates. Could they be? Sure. But our little exercise hasn't exposed anything shocking.
That was pretty much to be expected, I suppose, but eh. It turned out differently in the case of the GXP.
We'll keep watching this story for signs of life, of course, but I'm going to go ahead and close the poll. We'll replace it with this new one, which should be just as much fun.
This discussion is now closed.
|Here's an early look at DX12 "Inside the Second" benchmark data
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|Rumor: GeForce GTX 1060 specs leak
|Coolchip Technologies teases a low-profile "kinetic cooler"
|EKWB has a full-coverage water block ready for the RX 480


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Good Luck - Cross Stitch Pattern
Whispered by the Wind
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Item: WB71 - Type: Cross Stitch Patterns.
Model stitched on on 28 Ct. Ale Cashel Linen with Carrie's Creation Floss (Navy Blues, Gator Green, Gold, Raven, Olde World Red) or DMC Floss. Stitch Count: 93W x 93H. Variation was stitched on 28 Ct. Doubloon Cashel by Picture This Plus with Carrie's Creation floss (8 yards of Mojave Fire and 8 years of Rusty Nail).
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on 03/16/2015 - Another Winner
I like that there are two color variations available in this pattern. I am having trouble choosing which one I like the best. The chart is large enough and will be easy to stitch from. I think I am going to go with the "traditional" color format and start this one pretty soon.
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|DMC 310 Six Strand Floss by DMC
Black DMC floss. This quality embroidery floss is perfect for stitching on all types of fabric. Made with 100% long staple cotton. Double mercerized. Brilliant six-strand divisible thread. Colors are washable and fade resistant. Article 117.
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|DMC 3799 Six Strand Floss by DMC
Very Dark Pewter Gray DMC floss. This quality embroidery floss is perfect for stitching on all types of fabric. Made with 100% long staple cotton. Double mercerized. Brilliant six-strand divisible thread. Colors are washable and fade resistant. Article 117.
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Stagefright might help fix one of Android's biggest flaws
Last week, the Android world woke up to quite a scare. Imagine a vulnerability where you don't have to do anything at all. You just need to receive an MMS (multimedia message) and you're done for. That was Stagefright. And while Google was said to have responded quickly, revelation of the security hole also put one of Android's biggest warts under a spotlight once more. No matter how swiftly Google acts, updates don't come as fast. Now, however, Stagefright might ironically be helping change that, slowly but, hopefully, surely.


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Kobe Bryant takes baby steps on Instagram
Kobe Bryant is making good on his promise to return from a torn Achilles tendon as soon as possible and now he has a video to prove it.
The Lakers star used the new video feature on Instagram to show his first steps without a walking boot on his left leg. He's still using crutches, but it's progress.
He wrote:
June 1st. 7:30am. My first steps without the boot. Rockin the #medicalmambas Walkin like a baby giraffe. #achillesrupture #challenge #pray4bear #recovery
Bryant suffered the tear during the last minutes of a game April 13 and has periodically updated his fans about his rehabiltation through Instagram and Twitter.
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Draft and Trade Talk Message Board
Cano/Fielder/Hamilton/D. Wright -- Keep 3
Hey guys,
I'm worried about both Hamilton and Wright's consistency and health. I get to keep 3. What do you think?
I've also got Allen Craig and Kris Medlen (my pitching staff killed me last year, so I'd love a stud this time around).Sort: Newest | Oldest | Most Replied Expand all replies
Cano, Fielder for sure. Although I personally like Hamilton more than Wright, I'd probably keep Wright as my 3rd mostly due to how thin 3B is.
- 1 Reply to George
Thanks, guys! Any more thoughts. I'm really stumped here.
Hamilton had a weak second half last year -- but he's either hitting before or after Pujols with Trout on base.
Prince has a similar deal with Cabrera and seems to be much more consistent.
And David Wright is David Wright.
I'd throw back Fielder based on the depth at 1B. Medlen is no where near the value of your bats.
Cano and hamilton for sure. I would probably go fielder with the 3rd slot.
cano,wright and fielder, nice start, bob
And thanks in advance!
- 1 Reply to Jon Y.
I'd say let Wright go and just get the best 3B you can later on. Cano and Fielder are no brainers and eventhough Hamilton will be injured, he's still going to bat behind Trout and Pujols when he is healthy.
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text:
Bendis On War Machine's Death, She-Hulk's Fate & Raising the "Civil War II" Stakes
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Welcome to What Are You Reading? Today's special guest is writer and comedian James Asmus, who you know from Gambit, Thief of Thieves and the just-released The End Times of Bram & Ben.
To see what James and the Robot 6 crew have been reading, click below …
Typically, I'll spend most of Saturday in panels, but the first one I was interested in wasn't until later in the morning, so I killed time taking in some of the more offbeat exhibitors, like Ben the Bubble Guy, a businessman who hires himself out for birthday parties, corporate events, funerals. Okay, maybe not funerals.
When it was time, I headed up to the fourth floor for the AV Club's panel on the Future of Superheroes.
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Writer: SFC Forever
Date:Monday February 13 2012
Time: 7:43PM
Upton Park
Tues 14th Feb 2012
Table topping West Ham entertain second placed Southampton in what could be a very physical game with both sides desperate not to lose. West Ham can open a useful lead on the chasing pack with a win where as Southampton can overtake them if they manage a victory.
With so much at stake and with a fervent crowd roaring them on, West Ham are likely to start with a lot of gusto. Southampton looked to be more solid in their last couple of games and will be hoping to maintain a recent improvement of form.
The Hammers will be without Kevin Nolan through suspension and are likely to be without Papa Bouba Diop. This probably won't make much impact on the game as the squad has a host of quality players who can replace them when necessary.
Saints on the other hand are at full strength with the exception of Danny Butterfield and Dan Seaborne who are both out long term. The main problem facing Nigel Adkins will be who to select for the match day squad, and who will then be in the starting eleven.
With Nolan and Diop out it will be interesting to see who gets selected.
Green could be the difference between us scoring and not if he turns in one of his special performances.
Saints only have Butterfield and Seaborne injured so no problems for Nigel apart from who does he select to do the job.
Jos will need to be art his best to help keep our hosts away from our goal.
West Ham have a good record at home to Saints winning 24, drawing 11 and losing just the 9 matches.
The Hammers have won 5 of their last six home games drawing the other. Saints have collected 2 wins,3 draws and 1 loss from the last six.
Mr Lee Probert officiates Stuart Burt and Adam Watts assist Fourth Official Dean Whitestone
Hammers 2 - 2 Saints
Date:Monday February 13 2012
Time: 7:43PM
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iPhone - iPad Games
Stardom: Hollywood Answers for iPhone - iPadHome / iPhone - iPad /
Game Questions & Answers (Q&A) service lets you ask questions about video games for game consoles or PC games. So ask your Stardom: Hollywood question for iPhone - iPad and get answers from real gamers or answer questions and share your insights and experience with the rest of the gaming community.
Boxshot & Details
- Developer: Glu Games Inc.
- Publisher: Glu Games Inc.
- Genre: Role-Playing
- Release: Feb 13, 2013
- ESRB: Not Set
Question & Answers
Steal Newmanson Role
I'm meeting with Wilfred Watkinson about a movie and he's asking me to take over Newmanson's role. Should I do it? If I take it, will there be any negative repercussions later?
Answer from: Aj:) Btw a girl
I took it and my friend dumped me
Answer from: STArodm
I took the oppotunity to work in the movie.she dumped me and told me tat she would destroy my career .nothing really happens actually
Answer this QuestionAsk / All Questions
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- Poll of the Day
- Partners
Jun. 23, 2016, 12:41 pm EDT – As the 2016 election nears, a possible Clinton presidency weighs on Big Pharma. Profit Scanner identified three biotech stocks in particular. More
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Jun. 15, 2016, 8:54 am EDT – Rent-A-Center has a new downtrend in store — one with 31%–37% further downside. More
Jun. 8, 2016, 9:13 am EDT – Chuy's Holdings turned in a solid Q1 earnings report on May 3. Afterwards, CHUY consolidated those gains…then, on Monday, it broke higher again. That move confirmed a bullish Symmetrical Continuation Triangle on CHUY's chart. More
Jun. 8, 2016, 6:00 am EDT – Commodities have had it tough lately, but with a bottom in for oil, energy stocks EPE, JPEP and GNE are looking better and better. More
Jun. 1, 2016, 9:28 am EDT – The beleaguered retail stock, is starting to show signs of life. On Friday, it gave off three bullish technical signals, then in Tuesday's session it completed a Head and Shoulders Bottom. More
May. 25, 2016, 10:55 am EDT – Are prevailing concerns about big pharma stocks taking their toll, or are LLY, BMY and ABBV showing their strength anyway? More
May. 25, 2016, 9:16 am EDT – On Monday, Profit Scanner confirmed a bullish Continuation Diamond pattern on QLGC's chart — suggesting that, within roughly the next month and a half, the stock should see up to 19% further upside. More
May. 19, 2016, 12:05 pm EDT – There's always a bull market somewhere, and we've found these three bullish stock picks -- REGN, RTEC and TIS -- for your consideration. More
May. 16, 2016, 8:46 am EDT – Caterpillar shares had rocketed higher as investors saw opportunity once the downtrend in place since early 2011 finally gave in to upside pressures. More


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text:
Ikechi Anya starred for Scotland as they recorded an impressive 2-1 win over Macedonia in Tuesday's World Cup qualifier.
The Watford winger was the best player on the pitch in his first international start, and gave Scotland a deserved lead in the 59th minute.
The visitors had dominated the first half and were well worth their lead, but they looked like only getting a draw when Jovan Kostovski netted with seven minutes to play for Macedonia.
There was still time for the Scots to win it, though, as an 88th-minute free-kick from Shaun Maloney curled into the top corner.
Macedonia coach Chedomir Janevski made four changes to the side that beat Wales 2-1 on Friday, with Barcelona youngster David Babunski and attacker Adis Jahovic among the inclusions.
Anya made his first start for Scotland, who also brought in Barry Bannan and Steven Naismith in place of James Forrest, Leigh Griffiths and the suspended Robert Snodgrass.
Gordon Strachan's decision to include Anya looked a wise one, with Scotland dominating the first-half play in Skopje.
The pace and trickery of Anya caused Macedonia several problems, and he created the first chance of the game, crossing for Naismith, who could only head over.
The pair should have linked up again moments later, but Naismith agonisingly failed to get anything on a terrific cut-back.
The chances kept coming for Scotland, as Scott Brown was denied by a good save from Macedonia goalkeeper Tomislav Pacovski, while Anya had a late chance of his own, only to see his curling effort tipped over.
Scotland were nearly made to pay for their profligacy on the stroke of half-time, but Jahovic wasted a good chance.
Jahovic initially ignored Goran Pandev, who was waiting for a cut-back, before firing into the side netting, as the two sides went to the break on level terms.
Macedonia were improved in the second half, and saw much more of the ball, but Scotland - who were forced to substitute goalkeeper David Marshall at half-time with a hip injury - were still more threatening going forward.
Anya went close again with a curling effort, before the 25-year-old broke the deadlock in the 59th minute, racing on to a through ball from Brown.
Anya's shot had to be perfect, given the angle, and that it was, as his first-time finish nestled into the far corner.
Macedonia pressed for an equaliser, with substitute goalkeeper Matt Gilks making good saves to deny Aleksandar Trajkovski and Pandev as the hosts gathered momentum.
And they were lucky to have a penalty appeal turned down when Pandev fell under the attention of Brown.
Macedonia's pressure eventually told, as Pandev chipped a wonderful through-ball into the path of Kostovski, who finished well past Gilks.
But just as the hosts thought they had won a point, Scotland grabbed a dramatic late winner.
Maloney was the scorer, curling a brilliant free-kick into the top corner to give Scotland a deserved victory.
0 - 1
J. Kostovski 84'
(assist by G. Pandev)
1 - 1
1 - 2
89' S. Maloney
|T. Pachovski
|N. Noveski
|V. Shikov
|D. Georgievski
|S. Ristovski
|I. Tričkovski
|O. Stjepanovic
|D. Babunski
|G. Pandev
|A. Jahović
|A. Trajkovski
|Coach: Č. Janevski
|D. Marshall
|A. Hutton
|S. Whittaker
|C. Mulgrew
|R. Martin
|G. Hanley
|S. Maloney
|S. Brown
|B. Bannan
|I. Anya
|60' 75'
|S. Naismith
|Coach: G. Strachan
for D. Babunski 42'
for A. Trajkovski 57'
for A. Jahović 83'
for D. Marshall 46'
for B. Bannan 78'
for S. Whittaker 80'
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For many things in life, newer is better, but when it comes to books, there are a number of situations in which you may want to make books look old, or even antique. Antique books do not just look good...
While restoration of antique books often requires a passion or love for books in general, there are many particular reasons for restoring an antique book, from sentimental value to a proclivity for a particular...
Perhaps a set of rare dictionaries have been passed down to you, or you ve stumbled upon one of only a few remaining copies of a classic novel. Depending on your circumstances, selling rare books online...


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Otolaryngologist (ear, nose, throat)
41 years of experience
41 years of experience
251 Kukuau St
Hilo, HI 96720 Phone number & directions
Hilo, HI 96720 Phone number & directions
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Dr. Hamasaki graduated from University of Missouri at Columbia.
Home > Find an Otolaryngologist > HI > Hilo Otolaryngologists > Dr. Chalmers Hamasaki, MD
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- Additional Information
- Dr. Chalmers Ken Hamasaki MD is a male Otolaryngologist, has 41 years of experience and practices in Otolaryngology.
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Dr. Chalmers Hamasaki practices Otolaryngology near Hilo, HI.
See Hilo Otolaryngologists.


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Portland May Tax 'Non-Green' Building Projects
In Portland, new buildings that are not LEED Silver-certified or better may face a punitive tax, if a recent proposal goes through.
New commercial buildings 20,000 square feet or larger would fall under a so-called "feebate" program, in which developers that merely comply with Oregon's state building code would be charged as much as $3.46 a square foot. For buildings that achieve LEED Silver certification or better, the fee would be waived, and some developments would even qualify for a rebate of $1.73-17.30 per square foot depending on the certification level.
Portland's Bureau of Planning and Sustainability also may require buildings to achieve specific LEED credits, such as for energy efficiency and reducing water use.
The proposal has been met with some criticism, but it has earned the backing of the Portland Oregonian newspaper's editorial board.
Portland has landed atop a list of America's greenest cities.
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Forum indexRetouchingChange forum
Color Change and sports team logo
Started Apr 4, 2013 | Discussions thread
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Brock9911 • New Member • Posts: 1
Color Change and sports team logo
Apr 4, 2013
I happened to stumble across both these photos, but i can only seem to find this for the Steelers. I was looking to change the theme to the New York Rangers which is Red White and Blue, figuring the black could be the blue and the gold could be the Red and obviously put their logo where the Steelers one is. I am wondering how or what is the best way to go about doing that. Thanks!
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Face & Neck Revitalize - 2 oz
Home > Health & Beauty > Skin Care > Face & Neck Revitalize - 2 oz
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