text:
[ { "catentry_id" : "399866", "partnumber" : "11920973", "Attributes" : { "Color_Black":"1" , "Size_Standard":"2" }, "ItemImage" : "/wcsstore/ExtendedSitesCatalogAssetStore/PINGBLCKOUTGRIP", "ItemImage467" : "/wcsstore/ExtendedSitesCatalogAssetStore/PINGBLCKOUTGRIP", "ItemThumbnailImage" : "/wcsstore/ExtendedSitesCatalogAssetStore/PINGBLCKOUTGRIP" } ]
PING Blackout Putter Gripby PING SKU:PINGBLCKOUTGRIP PINGBLCKOUTGRIP
PING Blackout Putter Grip is rated 4.5 out of 5 by 8.
- bvseo_cps, prod_bvrr, vn_cps_3.2.1
- cp_1, bvpage1
- co_hasreviews, tv_1, tr_7
- loc_en_US, sid_PINGBLCKOUTGRIP, PRD, sort_relevancy
- clientName_golfgalaxy
- bvseo-188.8.131.52
- bvseo-true
- bvseo-j
- bvseo-CLOUD
- bvseo-34ms
- bvseo-REVIEWS
- bvseo-PRODUCT
- bvseo-getAggretateRating
Pre-Order Product:
This product will ship after . By continuing with your order, you accept these terms and conditions.
1. Color:The price of the product might be updated based on your selection
2. Size:
Add to Cart
if(typeof(shoppingListJSPRODUCT) == "undefined" || shoppingListJSPRODUCT == null || !shoppingListJSPRODUCT) { shoppingListJSPRODUCT = new ShoppingListJS({storeId: '10701',catalogId: '10051',langId: '-1'}, {id: '1325852', eCode: '16CWYUCLHYBRDLSRGRNG', name: 'Callaway Hybrid Laser-GPS Rangefinder ', image: '//s7d2.scene7.com/is/image/dkscdn///16CWYUCLHYBRDLSRGRNG', type: 'ItemBean', components: {}, skus: [], partNumber:''}, {}, "shoppingListJSPRODUCT"); }
- Sign In or Register to access your lists.
- Sign In / Register
Save for Later
In Stock
Select attributes to see availability
A favorite model of Tour professionals.
- .580" Core
- 58 gm
- Brand: PING
- Country of Origin: Imported
Rated 5 out of 5 by Madaja from My go to grip when things need to get dialed in I like small grips, which this is, they allow for the greatest amount of feel. I always seem to try different grips throughout the year but end up with this one for 75% of the time. I usually add two layers of tape just to give it a bit more size. These last forever and are the cheapest grips you can find, so value wise its the best deal by far.
Date published: 2014-10-23
Rated 1 out of 5 by Wildbanana from Nice and thin for smaller hands I like a short putter. Had this grip installed after shortening the shaft. Gripe was installed with me not being present. Unfortunately I have a non traditional grip so the flat spot was off for me by 5 degrees. I have to have redone but be there to set the flat spot. Will defenately have another ping installed
Date published: 2015-04-17
Rated 5 out of 5 by kevin from Great Feel! Awesome look. You can't beat it. Just changing your putter grip in general will improve your game, but this grip has a great feel and response. Plus, many of the best golfers in the world use this grip. (ie. Tiger Woods) You just can't go wrong.
Date published: 2011-04-19
Rated 5 out of 5 by Pwjackson from Good Product - Great Service Purchased a new grip for my 30 year old chipper. Staff replaced the grip with a new one. Once again I was very happy with the product and service. Staff at Golf Galaxy in Ft Myers Fl are terrific
Date published: 2015-08-13
Rated 5 out of 5 by dpgolf from An Excellent Grip! I just replaced my vintage Ping Anser 2 putter grip with this classic Ping Blackout Grip! I love the feel and look of this grip!
Date published: 2015-04-23
Rated 5 out of 5 by Marathon Man from Ping Putter Grip I bought this grip to replace my grip on a Ping putter. It match the original one. It feels great and the price was good.
Date published: 2015-04-17
Rated 5 out of 5 by Chaplain of the Green from The feel of the grip. Ping grips have always give to me confidence in playing the game.
Date published: 2015-05-14
- bvseo_cps, prod_bvrr, vn_cps_3.2.1
- cp_1, bvpage1
- co_hasreviews, tv_1, tr_7
- loc_en_US, sid_PINGBLCKOUTGRIP, PRD, sort_relevancy
- clientName_golfgalaxy
- bvseo-188.8.131.52
- bvseo-true
- bvseo-j
- bvseo-CLOUD
- bvseo-0ms
- bvseo-REVIEWS
- bvseo-PRODUCT
- bvseo-getReviews
*Price Promotions
Due to manufacturer restrictions, select new release and other specified products are excluded from price promotions. Additionally, there are restrictions on the use of coupon codes. Select products/brands include: Adams, Callaway, Cleveland, Cobra, ECCO, FootJoy, Mizuno, Nike, Odyssey, PING, Pinnacle, Scotty Cameron, SkyCaddie, TaylorMade, Titleist, Top Flite, Tour Edge Golf, Under Armour and Zero Restriction. Promotions are limited to one code per transaction. Cannot be combined with any other offers or coupons or used on previously purchased merchandise. Coupon valid on golfgalaxy.com purchases only. Not redeemable for cash, gift cards or store credit. No reproductions or rain checks accepted.
- © 2016 Golf Galaxy
- Order by phone: (800) 287-9060


text:
Taking a look at the television copy of Thursday night's preseason game against the Buccaneers and offering some second quarter observations:
1) Coming into the game in place of Dane Fletcher, MLB Gary Guyton looked stronger against the run than he did in his preseason debut against the Jaguars. On the second play of the quarter, Guyton met FB Erik Lorig at the line and quickly shed the block, wrapping up RB LaGarette Blount for a short gain. RDE Andre Carter also read the pump-fake draw play well to not get caught upfield and help force Blount back into Guyton. This set-up a third-and-long where a blitzing WLB Jerod Mayo came in free to combine on a sack of QB Josh Freeman with RDE Mark Anderson and LDE Rob Ninkovich, who both beat their blockers around the edge.
2) The Patriots' pass protection began to falter early in the second quarter, with pass rushers getting at Brady after two consecutive throws. The first came from RDE Adrian Clayborn, who stunted inside on LG Logan Mankins before beating Mankins back to the outside, falling into Brady's right leg. The second came from LDT Gerald McCoy, who beat RG Dan Connolly on a swim move to the outside and hammered Brady after a pass attempt to WR Chad Ochocinco. On the ensuing third-and-long, QB Tom Brady was flushed from the pocket after some stunting action from the Buccaneers' defensive line broke down the pass protection; Brady, on the run, was essentially forced to throw it away.
3) RB BenJarvus Green-Ellis' touchdown run that was negated by a holding call on C Dan Koppen was set-up on solid blocking from LT Nate Solder and a pulling Connolly, as well as some strength shown by Mankins on an inside seal block. When Green-Ellis officially scored two plays later, it was a solid outside seal block by TE Will Yeatman and a poor angle played by RCB Elbert Mack that allowed Green-Ellis to walk into the end zone.
4) The Patriots' defensive line continued to generate pressure into the second quarter, with QB Josh Johnson flushed out of the pocket on a third-and-20 by a stunting RDE Ninkovich, but still able to gain the first down on a 23-yard completion to WR Sammie Stroughter. On the next play, Andre Carter was the one to create the pressure, beating LT James Lee to the outside and hitting Johnson as he threw; the pass was nearly intercepted by Ninkovich.
5) Even using a four-man line, the Patriots weren't afraid to bring linebackers on blitzes throughout the game. This paid dividends in the pass rush, such as a third-and-15 late in the second quarter. Both LBs Mayo and Tracy White blitzed out of the nickel package, creating a 6-on-6 rush where Johnson had to avoid rushers before delivering a wobbly pass into the secondary.

subset: commoncrawl


text:
STI 7501D Polycarbonate Enclosure with Open Conduit Back Box, Surface Mount Applications and Interior Thumb Lock
Custom Order
Custom orders usually ship in 1-2 weeks.
This multipurpose lockable enclosure is available to protect a wide range of larger size devices, components and sensitive equipment, such as keypads, annunciators, volume controls and lighting controls that require physical or environmental protection.
The STI-7501D guards sensitive devices from vandalism, accidental damage as well as damage caused from exposure to the elements. Spacer, hinges and cover are formed from super tough polycarbonate material. The polycarbonate cover is backed by a three year guarantee against breakage in normal use. This STI Enclosure is lockable with a thumb lock (key lock models also available - see STI-7500 series).
Also known as
On Sale:E-mail a Friend


text:
It's a pretty little inn really calm. The place it's very exotic and the owners work very hard to keep it clean and nice. We loved the landscape, especially a palm tree leaning over the sea. It makes great pictures and souvenirs. We sat on it to admire the endless expanse of the sea. And the beach is just ..... More
What is Certificate of Excellence?
TripAdvisor gives a Certificate of Excellence to accommodations, attractions and restaurants that consistently earn great reviews from travellers.


text:
Facelift Q&A
6 Months Post-op + Facelift
Six Months After Full Face Lift and Eyes Surgery...
I did my full face lift and eyes lift surgery 6 months ago in KL. Happy with the results. I went to see my surgeon 4 months ago to make sure... READ MORE
Why Didn't My Facelift Work? (photo)
Hi There. I am 44 years old, athletic, non smoker or drinker, healthy and have never had a problem healing. Six months ago I had a mid facelift,... READ MORE
Need a Redo After MACS Face Lift Jowls Not Lifted? (photo)
I had a macs face lift six months ago.I have healed well but my chin is not defined.My surgeon would llke to redo in order to lift the jowl and... READ MORE
Bruising After 6 Months Post Op Facelift and Neck?
It has been 6 months since facelift and neck. There is still briusing along the jawline will this resolve itself and if so how long? READ MORE
Facial and Neck Pain Are Severe 6 Months After Facelift, Necklift, Chin PImplants, What Should I do?
I had a facelift,necklift,chin implant and fat injections. The pain in my face and neck are growing worse. In addition, my gums and inner lips have... READ MORE
Marks That Look Like Persistent Bruising After Bleph, Browlift and Mini-face Lift?
I had upper and lower eyelid, browlift and mini face lift approximately 6 months ago. I've noticed I have what looks like shadow/bruise rings below my... READ MORE
Face Lift and Still Looks the Same? (photo)
I had a face lift and face fat removable six months ago,three months out i look the very same.The Dr was not as happy with face but only say wait and... READ MORE
Can a Lower Facelift & Platysmaplasty Cause or Exacerbate TMJ Dysfunction?
I had a lower facelift 6 months ago and I have a lot of uncomfortable tightness around ears, jaw and back of head. Previous to the surgery I had a... READ MORE
Why Do I Still Have Extreme Pulling, Pressure and Tightness 6 Months After Facelift Surgery?
I am 6 months post mid facelift, neck lift, upper and lower blephoplasty, endoscopic brow lift and cheek implants. I am still experiencing extreme... READ MORE
Is my Result Reasonable or a Total Failure? (photo)
I am re submitting my question of 2 weeks ago with better photos. After shots are 6 months post op. Wish I could put more but only seems to be option... READ MORE
In your opinion how successful is the closed platysma band technique which requires only a local anesthesia?
Since having a mini face lift and neck liposuction 6 months ago, I have very visible platysma banding. I would like to avoid general anesthesia for a... READ MORE
I Had a Face & Brow Lift 6 Months Ago. I Have Bumps Under my Eyes & in the Corners. What Causes Thus & What Can Be Done? (photo)
I have submitted a photo to help you see the problem. Thank You! READ MORE
White patch behind ears 5.5 months after facelift?
I am 5.5 months post-facelift and have noticed a white bleached patch behind my ear. I am worried about skin necrosis because I am a smoker, although... READ MORE
What is the best treatment for scarring and creping after SOOF lift? (photo)
A friend of mine had a SOOF lift performed in June this year (roughly 6 months ago) she has quite bad scars on each side of the eye which are uneven... READ MORE
I had a facelift done 6 months ago and have irregularities on the left side of my face. What can I do? (photo)
It looks like malar bags under left eye dr. Said Its your cheek! Left cheek falling in under cheekbone. Dr. Did say he would do some filler around eye... READ MORE
I have scar tissue that developed after a face lift and neck band surgery. What can be done? (Photo)
The surgeon saw me after six months and agreed that there was scar tissue and to try massage. He agreed with me that a moderate level dermapen... READ MORE
Will face return to its pre-suture lift state over time?
I had a Silhouette suture lift about 6 months ago. The sutures were anchored to my deep fascia, but I had the knots (tension) cut at 6 weeks because I... READ MORE
I Had a Facelift 6 Months Ago & I Have Bumps Under my Eyes & in the Corners. What Can Be Done to Correct These?
I had a full facelift, but did not have my eyes done. I did have a brow lift, also. I had bumps immediately after surgery & they have gotten worse... READ MORE
I have excessive blinking after full face lift. Is this normal?
I had a full face lift six months ago including eyes and brow lift. I am blinking excessively and as it gets worse I tend to make facial movements. My... READ MORE
When will it be safe & Where do I go to fix my botched surgery? (photos)
6 months ago I had facelift surgery by a Board Certified Plastic Surgeon in Dallas, TX. I am unhappy because the jowls are still there. I posted... READ MORE


text:
Family Asks North Korea To Release 85-Year-Old American
The family and friends of an 85-year-old California grandfather have appealed to North Korean officials to release the man, who reportedly has been held by authorities in the communist state since Oct. 26.
Merrill Newman, a veteran of the Korean War who traveled with a friend to North Korea last month, was "removed from a plane three weeks ago" just minutes before he was to depart on the first leg of his trip home, the San Jose Mercury News writes.
Jeffrey Newman, the missing man's son, tells The Associated Press it appears that a North Korean military officer boarded the plane, asked the elder Newman for his passport and then said the American had to leave the aircraft. "My dad got off, walked out with the stewardess, and that's the last he was seen," Jeffrey Newman says.
The scene was witnessed by Merrill Newman's friend, Bob Hamrdla. The two men had traveled to North Korea together. Hamrdla was allowed to depart.
The Mercury News adds:
"The day before he was to depart, Newman met with North Korean officials, who discussed his Army service in the Korean War more than a half-century earlier ... Jeffrey Newman said Wednesday night.
"Merrill Newman was slightly unnerved, his son said, but went to dinner and thought nothing of it until the next day ... when he was escorted off the plane. ...
"Hamrdla released a statement Wednesday afternoon, calling Newman's detention 'a terrible misunderstanding.' "
"We don't know what this misunderstanding is all about," Jeffrey Newman also tells the AP. "All we want as a family is to have my father, my kids' grandfather, returned to California so he can be with his family for Thanksgiving."
He says his father had for years wanted to visit the Korean peninsula to see some of the places where he served during the war decades ago.
The wire service writes that:
"North Korea has detained at least six Americans since 2009, often for alleged missionary work, but it is unusual for a tourist to be arrested. ...
"Speaking Thursday to reporters in Beijing, U.S. Special Representative for North Korea Policy Glyn Davies wouldn't confirm Newman's detention but said, generally, that Washington was working with the Swedish Embassy in Pyongyang, which acts as America's protecting power because Washington and Pyongyang don't have official diplomatic relations, 'to try to move this issue along and of course calling on North Korea ... to resolve the issue and to allow our citizens to go free.' "
You must be signed in to leave a comment. Sign In / Register
Please keep your community civil. All comments must follow the NPR.org Community rules and terms of use, and will be moderated prior to posting. NPR reserves the right to use the comments we receive, in whole or in part, and to use the commenter's name and location, in any medium. See also the Terms of Use, Privacy Policy and Community FAQ.


text:
Discover a selection of top forums rider blisset on the internet. Come to share your passion on forums rider blisset and meet other fans of rider blisset. Then, why don't you create your own forum of rider blisset ?
An Unofficial Line Rider Community, previously known as TechDawg's I Ride the Lines
line, #rider, ride, lines, irtl, wrtl


text:
John Piecuch was appointed non-executive chairman of Houston-based U.S. Concrete Inc. He has been a member of the board since 2007 and most recently was president and CEO of MMI Products Inc., a manufacturer and distributor of building and construction materials. Piecuch succeeds Vincent Foster, who remains on the board. For more, visit www.us-concrete.com.
Building Materials
Now there's a greener alternative to disposable plastic sheeting.
Houston-Sugar Land-Baytown, TX
As employment grows, 25 percent of firms report labor shortages are forcing them to...
Federal Housing Administration
As part of these transactions, CEMEX paid €45 million in cash to Holcim. CEMEX expects...
Insteel Industries, Inc. announced that its wholly-owned subsidiary, Insteel Wire...
Please read our Content Guidelines before posting.
Copyright ©
2016 Hanley Wood Media, Inc. All rights reserved.


text:
After the Hurricane: What's Up with the Reporter Who Took Down Jayson Blair?
Anders, Gigi, American Journalism Review
"I'm just a brown girl down here in the valley, trying to figure things out and make sense of them," Macarena Hernandez says. It's mid-afternoon on a sweltering summer day in Edinburg, Texas, and the Rio Grande Valley brown girl and unsweetened iced-tea addict who inadvertently became a journalistic mega-catalyst is talking from her cell phone. "Hold on a second," Hernandez says, pulling up to Whataburger, a fast-food drive-thru Texas staple. "Yeah, I'll have the unsweet tea. Extra-large. And extra, extra ice. Thanks. Sorry. I go out at least once a day for unsweet tea. I looove it. I won't make it at home 'cause if I did I'd just drink the whole thing in one sitting. This way I have to take it one gulp at a time."
Taking it one gulp at a time is how the San Antonio Express-News special-projects reporter has always approached her vida loca--and it has been loca, particularly since April of last year, when Hurricane Jayson blew through. The erstwhile and disgraced New York Times reporter Jayson Blair garnered most of the media attention. First for his serial plagiarism, fabrications and lies, which brought down the Times' leadership, and later for his nonstop book-hawking blitz.
Though Blair was an equal-opportunity thief, stealing from everyone, it was ultimately the theft of one of Hernandez's articles that sealed his doom. "He stole my story and my voice," says Hernandez, 29. "It pissed me off. I was like, 'You know what? You can't do that."
A brief recap: The story Blair stole had originally run in the Express-News on Friday, April 18, 2003. It was about Juanita Anguiano, a Los Fresnos single mother whose firstborn and only son, Edward, was the last American soldier MIA in Iraq. Hernandez spent hours with the anguished and vulnerable woman, who sadly showed the reporter the final gifts her son had given her. A tennis bracelet. A set of Martha Stewart patio furniture. A ceiling fan.
Eight days later, early on Saturday, April 26, Hernandez read online a New York Times story by Blair about Anguiano in which he described her pride in her "Martha Stewart furniture out on the patio." When Hernandez had been there, the furniture, like the bracelet, was still in its original box. A mother with a missing son halfway across the world having the energy and interest to assemble patio furniture by herself? And be proud of it?
Hernandez didn't think so, and told her editors so. (See "All About the Retrospect," June/July 2003.) The next day, Sunday, Edward Anguiano's family was notified that his body had been recovered. Monday, the family wasn't speaking to the media, who by then were swarming the house's front yard. Among the reporters there were Hernandez and the Washington Post's Manuel Roig-Franzia. The two struck up a conversation.
"We talked and I told him what Jayson had done," Hernandez says. "I'd torn his story apart, diagramming the similarities. There was no doubt about it. It was my story. The plagiarism was extremely obvious, stealing lines and phrases that I had written verbatim.... Manuel contacted the Post and four hours later [Washington Post media writer] Howard Kurtz was calling my cell."
Hernandez then called her old New York Times mentor Sheila Rule, who had hired both her and Blair five years earlier as interns. "I told her, 'I don't know how big of a deal this is going to be, but I want to give you a heads-up. Jayson Blair stole big chunks of one of my stories and Howard Kurtz is already on it.'"
Later that afternoon, Blair called Hernandez, supposedly to double-check a quote the mother had given him in Spanish that had been translated into English by the daughter. He asked for a copy of Hernandez's original story, which he claimed never to have seen.
"At that moment I knew he had never been there," Hernandez says. "Because the mother spoke English. I thought, 'Oh my God, this is bad.'"
The denouement, like the build-up, was fast and fierce, disturbing and then dominating Hernandez's life. …


text:
Shop faster, shop smarter with Curbside Express. Order online & pick up your groceries!
Pizza, Thin Crust, Bacon, Spinach & Artichoke A fusion of flavors. Artichoke hearts, spinach, bacon and mozzarella cheese with garlic white wine flavored sauce on a thin crust. US inspected and passed by Department of Agriculture. Experience the fusion of flavor. We're taking fresh taste to new heights, fusing together creative combinations of flavorful vegetables and signature sauces for exciting new taste experiences. Spinach, artichokes, and sundried tomatoes are topped with finer cheeses like asiago, feta and provolone on a thin and crispy crust. It's the Fresch idea in frozen pizza. Made in USA.
Please enter the UPC found on the product package.


text:
Apply for a work permit
Follow these steps to apply for a work permit.
1. Determine if you will apply online or on paper
Apply online
You must have:
- access to a scanner or camera to make electronic copies of your documents to upload, and
- a valid credit card to pay with.
Determine your eligibility and documents required to apply online.
Note: If you provide an email address on your application, please check your email account often for automated emails from CIC regarding your case. Some spam filters block these emails. Make sure that emails from CIC are not blocked. If you are not sure whether emails from CIC are being sent, please check your myCIC account often.
Apply on paper
Follow steps 2 to 5 below.
Note for IEC applicants: If you are an applicant under the International Experience Canada program, you can only submit your work permit application online. Paper applications will not be accepted.
2. Get the documents from your employer
Before you apply for a work permit, you will need:
- the positive Labour Market Impact Assessment (LMIA) your employer received from Employment and Social Development Canada if you do need an LMIA, and
- your job offer,
- the ID number your employer received when they submitted the offer of employment through the Employer Portal if you do not need an LMIA and you are not applying for an open work permit
Note: As of November 21, 2015, we no longer accept the IMM 5802 Offer of Employment to a Foreign National Exempt from the Labour Market Impact Assessment form. If your employer has given you a completed copy of this form, you must ask your employer to resubmit the offer of employment online through the Employer Portal.
You will also need:
- proof that you meet the requirements of the job, such as:
- proof of education, or
- work experience.
3. Get the application package
The instruction guide has instructions on how to fill out the application. It also tells you which documents you will need to submit to get your work permit.
There are different ways to apply for a work permit, depending on where you apply from:
- inside Canada
- outside Canada, or
- as you enter Canada.
Find out the requirements for where to apply in order to choose the best option for you.
Passport and entry into Canada
To work in Canada, you must meet the requirements for a work permit. You may also need an Electronic Travel Authorization (eTA) or a visitor visa, depending on your nationality. The eTA or visa will be issued at the same time as your work permit. You do not need to make a separate application or pay an additional fee.
4. Pay the application fees
In many countries, you can pay the fee in the local currency. More information about this and the payment methods available to you can be found by visiting the pay your fees tool.
Please note that if you apply on paper at a VAC, you will have to pay the VAC services fees. These fees are not included in your CIC application fees.
5. Submit your application
Note: Families should send their applications in one package to the location where the principal applicant will be processed (based on their type of application).
Related links
How-to videoA Step-by-step look at biometrics
Where to send your applicationFind out where you should send your application
- Date Modified:


text:
Carli Lloyd
Position: Setter
Height: 5-11
Hometown/Residence: Bonsall, Calif.
Birth Year: 1989
College/Major/Grad Year: University of California-Berkeley/Social Welfare/July 2011
Joined Team: January 2011
- Set Team USA to the 2015 Pan American Cup gold medal and was the leading setter in running set average at 6.27 per set
- Named the 2015 Most Valuable Player at the Pan American Games
- Started four of five matches at 2011 Pan American Games in helping the U.S. win the bronze medal…averaged 4.67 assists, 1.17 points and 0.50 blocks per set while converting six of nine errorless attacks for kills
2015 - USA Volleyball Cup vs China...Pan American Cup (Gold)...Pan American Games (Gold).
2012 - Pan American Cup (Gold).
2011 – Pan American Games (Bronze).
- Her uncle Galen Tomlinson, who was Turbo in the 1980s television series American Gladiator, was her mentor and coach in both volleyball and track and field in her youth. After the passing of Lloyd's father when she was four years old, Tomlinson moved in shortly afterwards and came to be a father figure for her. To read more about the relationship, click here
- Led U.S. Women's National A2 Team to the gold medal at the 2010 USA Volleyball Open National Championships and selected to the all-tournament team
- Became first player to become a three-time selection for the U.S. Women's National A2 Program
- Named all-tournament at the 2009 USA Volleyball Open National Championships after leading her USA A2 squad to the bronze medal.
- Helped her USA A2 squad to the gold medal at the 2008 USA Volleyball Open National Championships, earning all-tournament in the process.
- Majored in Social Welfare at the University of California-Berkeley
- Named American Volleyball Coaches Association (AVCA) Division I Player of the Year and All-America First-Team after leading University of California-Berkeley to a runner-up finish in the NCAA Division I Women's National Championship
- Became second-player in school history to receive three AVCA All-America honors
- Captured three all-tournament honors in final three seasons of competing in the USA Volleyball Girls' Junior National Championships
- Grandfather Doug Tomlinson was a former Major League Baseball pitcher for the Cleveland Indians
Related Links
- TeamUSA.org: Lloyd Leads Team USA to First Pan Am Games Gold in 48 Years (July 26, 2015)
- ESPN.com: U.S. Women Ready for Pan Am Games Final Versus Brazil (July 24, 2015)
Last Updated: Sept. 2, 2015
U.S. Women's Update: Gibbemeyer Near Perfect Week
Bill Kauffman April 08, 2014
Team news from around the world
U.S. Women's Update: Glass Gets Golden
Bill Kauffman April 01, 2014
Team USA news from around the globe
U.S. Women's Update: Hildebrand Honored in Brazil
Bill Kauffman March 25, 2014
Team USA news from around the world
U.S. Women's Update: Hooker Signs Book Deal
Bill Kauffman March 18, 2014
Team news from home and abroad
U.S. Women's Update: Past Meets Present
Bill Kauffman March 11, 2014
U.S. Women's Update: Red-Blue Match Friday
Bill Kauffman March 04, 2014
Red-Blue Match Review of Feb. 28 and Preview of March 7
U.S. Women's Update: Larson-Burbach to CEV Final 4
Bill Kauffman Feb. 12, 2014
Team news from around the world
U.S. Women's Update: Hagglund Interviews Davis
Bill Kauffman Feb. 04, 2014
U.S. Women's Update: Haneef-Park Earns Honor
Bill Kauffman Jan. 28, 2014
Team notes from around the world
U.S. Women's Update: See Who is Excited
Bill Kauffman Jan. 21, 2014
Latest news on the U.S. Women's National Team
Related Articles
More Stories ›


text:
Latest News
Street Style
Trending news
Top 10! Celebrities Who Once Were Poor And Homeless!
10 Celebrity Couples With Huge Height Differences!
Where Are They Now? America's Next Top Model Winners!
Here's How Vanessa Paradis Reacted On Johnny Depp's Engagement!
Shopping Mood in Hollywood
Copyright © 2016 Posh Media Group. All Rights Reserved.


text:
Artist Collection: (40 of 90)< Previous | Next >
Tags: nude, oil, painting, bird, yellow, woman, figurative, relism.decor,
- + MOUSE OVER image to zoom in
- + CLICK to enlarge
descanso 2by jose de la barra
- Medium
- Traditional
- Artist Notes
- Painting original on canvas available in rolled tub or streched, free shipping.
- Contact Artist Send Artist an Email
More about jose de la barra
- Location
- lima, per"∫
- Artist Biography
- Profesional peruvian artist, degree Fine Arts Schools 1984, Lima, Peru.  Work inspirid in the realism imaginary with special  woman and man in the fantasy world. Prize: International Art Contest SOHO 1999, nEW yORK usa.


text:
Affirmative Action Settlement; Excerpts From Statement By School Board Lawyer On Lawsuit's Settlement, N.Y. TIMES, Nov. 22, 1997, at B4.
B. Drummond Ayres, Jr., University Regents in California Battle Over Affirmative Action, N.Y. TIMES, July 21, 1995, at A1.
Barry Bearak, Affirmative Action Settlement: The Reaction; Settlement Ends High Court Case On Preferences: Rights Groups Ducked A Fight, Opponents Say, N.Y. TIMES, Nov. 22, 1997, at A1.
Joan Biskupic, Rights Groups Pay To Settle Bias Case; High Court Affirmative Action Ruling Avoided, WASH. POST, Nov. 22, 1997, at A1.
William Booth, U. of Calif. Ends Racial Preferences: Pioneer in Diversity Adopts Stance Urged by Gov. Pete Wilson, WASH. POST, July 21, 1995, at A1.
Ethan Bronner, U. of Washington Will End Race-Conscious Admissions, N.Y. TIMES, Nov. 7, 1998, at A12.
DeNeen L. Brown, Gray in the Debate on Color: Many See Both Sides of Affirmative Action, WASH. POST, June 5, 1995, at A1.
Tom Brune, Now That I-200 Is Law, What's Next?—UW Alters Admission Policy, SEATTLE TIMES, Nov. 5, 1998, at A1.
Stephen Buckley, Voting Rights Ruling Called Death Knell for Exclusion; Ex-Clinton Nominee Hails Order in Maryland, WASH. POST, Apr. 7, 1994, at B1.
William Claiborne, Affirmative Action Ban Is Upheld; California Proposition Constitutionally Valid, U.S. Appeals Panel Says, WASH. POST, Apr. 9, 1997, at A1.
Ruth Colker, Anti-Subordination Above All: Sex, Race and Equal Protection, 61 N.Y.U. L. REV. 1003 1986).
Ellis Cose, After Affirmative Action: Proposition 209 May Become Law, But Californians Are In No Rush To End All Racial Preferences, NEWSWEEK, Nov. 11, 1996, at 43.
Maura Dolan, U.S. Panel Upholds Prop. 209 Affirmative Action: Three 9th Circuit Justices Rule That Measure To Eliminate Preferences For Women And Minorities In College Admissions And Government Employment Is Constitutional; Opponents Will Appeal, But Some Analysts Believe They Face An Uphill Battle, L.A. TIMES, Apr. 9, 1997, at A1.


text:
Troy Brosnan: 3:17.84
Tracy Hannah: 3:28.14
Added a comment about feature WIN A ROCKSHOX BOXXER - Vital OTB, Lourdes World Cup 4/9/2016 6:42 AM
Troy Brosnan: 3:17.84
This feature has 771 comments.
Liked a comment on the item First Look: 2016 YT JEFFSY 4/7/2016 7:18 AM
JEFFSY Suspension analysis :D
Added a comment about slideshow Enduro World Series Race Action from Argentina, Stages 1-3 4/3/2016 5:05 PM
always the best content/race coverage.
Members who like this comment
- 8186 judgerider68 http://p.vitalmtb.com/photos/users/8186/avatar/c50_IMG_20160426_093426_1463195019.jpg?1463194567 http://www.vitalmtb.com/community/judgerider68,8186/all 05/18/11 1 1 17 145 2
- 33279 CuddlyToast /images/default/avatar/c50.png http://www.vitalmtb.com/community/CuddlyToast,33279/all 11/12/14 http://www.vitalmtb.com/community/CuddlyToast,33279/setup 2 28 8
This slideshow has 2 comments.
Added reply in a thread What Are YOU Most Picky About Regarding Setup? 2/28/2016 5:24 PM
Just a little deadband in my levers before they engage. That and noise free drivetrain. Can't stand noise from the BB & cranks specifically.
Added reply in a thread MTB Advice I'd Give Myself Five or Ten Years Ago 2/10/2016 2:55 PM
1. Never leave a buddy behind. not ditching a buddy is more important than who can get to the bottom or top fastest (unless your road riding ... because that's all that matters). 2. Make fun of Lycra as much as possible because at some point you'll own ... more »
Added a comment about photo 17 Bikes Tested - 2016 Vital MTB Test Sessions 1/19/2016 8:38 PM
Solid price for that weight and frame size.
This photo has 17 comments.
Added reply in a thread What Would You Do for MTB if You Won the $1,300,000,000 Powerball Jackpot? 1/12/2016 2:33 PM
1. Start a fund for riders who are injured competing. Kind of like Aflac but they don't have to pay in to it. 2. Disappear
Added reply in a thread dinged rim tubeless tire 1/11/2016 8:24 AM
admittedly I did not read all of the comments however I have experience with this so I figured I'd share. I dented my i9 enduro rim badly in two places and the tire would not seal. I took the tire off then put it in a truing stand and started bending ... more »
Updated bike check Banshee Darkside 1/7/2016 7:34 AM
Updated bike check Banshee Darkside 1/7/2016 7:34 AM
Added reply in a thread 2016 Racing Rumours 12/30/2015 1:14 PM
Zink had a pretty obvious tweet today
Added reply in a thread 2016 Racing Rumours 12/28/2015 8:33 AM
YT is a proven winner, just not in DH.
Added a comment about video All Stories End // Trek World Racing's Final Chapter 11/17/2015 7:47 AM
Grammar police: 3:34
This video has 1 comment.
Liked a comment on the item Opinion: When Does Risk Outweigh the Reward? How Red Bull Rampage Changed Our Perspective 10/19/2015 6:51 AM
What about charging $5 for every live feed? Have that money go straight to rider safety and secondarily to better purses. Red bull gets to splash their logo on everything and we pay the riders something for their risk. Honestly, I'd feel better watching...more
Added a comment about feature Opinion: When Does Risk Outweigh the Reward? How Red Bull Rampage Changed Our Perspective 10/19/2015 6:50 AM
The guy that started it seems engaged in our community so most likely it is but you never know. I read that Pauls family is going to put a fund together.
This feature has 88 comments.
Added a comment about photo WINNING BIKE - Kurt Sorge's Polygon Collosus 10/17/2015 7:43 AM
No bars?
This photo has 5 comments.
Added a comment about photo Tyler McCaul's El Presidente Step Down - 57 feet lip to landing 10/15/2015 2:01 PM
This photo has 7 comments.
Added a comment about feature First Ride: Commencal Supreme DH V4 9/9/2015 10:12 PM
Pretty interested in the 160mm mule
Members who like this comment
- 109 bturman http://p.vitalmtb.com/photos/users/109/avatar/c50_twitter3_1382472127.jpg?1382471385 http://www.vitalmtb.com/community/bturman,109/all 08/01/09 1156 92 5848 1058 http://www.vitalmtb.com/community/bturman,109/setup 252 1090 2071 124 40
- 74 Primoz /images/default/avatar/c50.png http://www.vitalmtb.com/community/Primoz,74/all 08/01/09 126 312
- 30616 bblackyeti1 http://p.vitalmtb.com/photos/users/30616/avatar/c50_IMG_1628_1463705145.jpg?1463704201 http://www.vitalmtb.com/community/bblackyeti1,30616/all 04/18/14 9 2 3 12 128 1
- 33466 schuster19 http://p.vitalmtb.com/photos/users/33466/avatar/c50_SST_head_shot_1454125139.jpg?1454124974 http://www.vitalmtb.com/community/schuster19,33466/all 12/02/14 3 20
This feature has 5 comments.
Added a comment about photo SRAM Wireless Shifting and Derailleur 9/4/2015 9:19 AM
only for components really needed to make the switch
no special compatible frame needed
no wire
modular batteries
45min charging
simple shifting
home run
This photo has 2 comments.
Added a comment about slideshow RACE REPORT: Oregon Enduro Series, Mt. Bachelor 8/28/2015 12:29 PM
Rattlesnake is a super cool and unique trail. Big time fun was had there.
This slideshow has 1 comment.


text:
I did my first race today since I got back on the bike about a year ago. It was the WV state time trial championship. Unfortunately I had to race cat 5 since I didn't renew my license this year, their rule, defiitely not mine. If I had my choice I would race cat 6.
The course was 15.2 miles, out and back course with a 5 mph(ish) cross wind. All in all I am pretty happy with my time. I still have a sinus infection that is making it tough for me to breath, my second day on the time trial bike, and I'm still fat and as aero as a brick. Certainly not making excuses, just noting what is going on.
15.18 miles
38:24 (minutes:seconds)
271 feet elevation gain
23.8 avg moving speed
291 watts avg (296 watts on WKO+)
311 nom watts
753 max watts
95 avg cadence
671 kilojoules
Please click on lap 1 for the numbers. http://connect.garmin.com/activity/113343628
Strava data - http://app.strava.com/rides/1574628


text:
STARKSBORO — The struggles of a Starksboro man in trying to navigate the Vermont Health Connect website are a snapshot of what the Shumlin administration faces while implementing the state's new health care platform.
Jeff Keeney first attempted to sign up for health insurance for his family in early November, and he still hasn't been able to complete his application. Exasperated, he fired off an email to Gov. Shumlin last week and copied a number of local lawmakers.
The full text of this article is only available to online subscribers.
Are you an online subscriber? Click to login.
Click here to learn more about our subscription options.
If you have a print subscription, you can now add an online subscription at no additional charge! Just email us with your name and mailing address to set it up.
Mount Abraham Union High School counselor Deb Van Schaack hiked the 2,200-mile Appalachian Trail this spring and summer. She began her journey in Georgia on March 8 and completed the trek in Maine on July 31. Van Schaack shared with the Independent some of the photos she took along the way.
BRISTOL — Of the people who begin hiking the Appalachian Trail with the intention of covering the entire thing, only one in four complete it. Most are young, recent college graduates, students taking a gap year, all with young joints and plenty of energy.
Not many 56-year-old women attempt the nearly 2,200-mile, 14-state trek, let alone complete it.
STARKSBORO — In 1965, Lyndon Johnson was president, Apollo 11 had yet to land on the moon, a first-class stamp cost a nickel and a gallon of gas 31 cents.
It was also the year Joyce Shepard started working as a cook at Starksboro's Robinson Elementary School. She retired in this past summer, after 48 years.
"I just liked working with the kids," Shepard said. "It kept me young."
Principal Patrick Hartnett likened Shepard's tenure to that of a baseball Hall of Famer.
STARKSBORO — Before this year, Eric Rozendaal had never been to Brooklyn, N.Y. Now, he's visited a dozen times — not as a tourist, but as a vendor of authentic Vermont food at an increasingly popular New York City market.
Rozendaal and his wife, Keenann, own Rockville Market Farm in Starksboro. Every weekend, he travels to "Smorgasburg," a weekly outdoor food market held in Brooklyn. Smorgasburg hosts 75 to 100 food vendors every week at two locations in the city's second-most populous borough.
STARKSBORO — Unbound Grace, a Starksboro-based nonprofit, will host a community benefit on Saturday, Sept. 21. Its fourth annual Community Big Barn Dance will include a country potluck with chicken BBQ, family field days, a silent auction, and all-ages square and contra dancing with a little bit of rock-and-roll. Music is provided by talented local band Gumbo YaYa. The event will be held at Sentinel Farms, 4118 VT Route 116 in Starksboro, from 3 to 9:30 p.m. Attendees may want to bring lawn chairs or a blanket.
STARKSBORO — Twelve years ago the property on Robert Young Road purchased by Price and Marijke Niles for their retirement was full of grass, brambles and brush.
"It was so bad, the real estate agent told us not to buy it," Marijke Niles, 67, recalled. "But we fell in love with the big rock, the view and the pond, and I wanted a big open space where I could garden."
STARKSBORO — Those who have unhappily watched the trends of young people leaving the state and farms closing up shop have reason to celebrate the launch of Footprint Farm in Starksboro this year.
Three young farmers — St. George native Jake Mendell, 25, and California natives Taylor Hutchison, 25, and Nathan Hammer, 32 — started work in April on their diversified, 9-acre farm at Starksboro's Common Ground Center.
And already the trio says they have found a warm welcome for their mix of vegetables, flowers and animals.
STARKSBORO — After more than two decades at Robinson Elementary School, art teacher Vera Ryersbach is retiring this month. She leaves quite a legacy behind — when Ryersbach came on board as a volunteer in 1989, Robinson was the last school in the Addison Northeast district that did not have an art teacher.
Subscribe to Online Edition
Online PDF Print Edition
Copyright 2011 Addison Independent | 58 Maple Street, Middlebury, Vt. 05753 | All Rights Reserved
Site Development by Surf New Media


text:
PubMed Submission Abstract PDF Cover Contents Editorial Board Count: 2812 Download Count: 371
Silvia Turroni, Beatrice Vitali, Marco Candela, Patrizia Brigidi, Department of Pharmaceutical Sciences, University of Bologna, via Belmeloro 6, 40126 Bologna, Italy
Paolo Gionchetti, Fernando Rizzello, Massimo Campieri, Department of Internal Medicine and Gastroenterology, University of Bologna, Polyclinic S. Orsola, via Massarenti 9, 40138 Bologna, Italy
Author contributions: Gionchetti P, Rizzello F and Campieri M directed patient recruitment and follow up; Turroni S, Vitali B and Candela M performed the research; all authors contributed to editing of the manuscript and approved the final version of the paper.
Correspondence to: Patrizia Brigidi, Professor, Department of Pharmaceutical Sciences, University of Bologna, via Belmeloro 6, 40126 Bologna, Italy. firstname.lastname@example.com
Telephone: +39-51-2099743 Fax: +39-51-2099734
Received: August 6, 2009 Revised: September 8, 2009
Accepted: September 15, 2009
Published online: January 7, 2010
AIM: To profile protein expression in mucosal biopsies from patients with chronic refractory pouchitis following antibiotic or probiotic treatment, using a comparative proteomic approach.
METHODS: Two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry were used to characterize the changes related to antibiotic therapy in the protein expression profiles of biopsy samples from patients with chronic refractory pouchitis. The same proteomic approach was applied to identify differentially expressed proteins in the non-inflamed pouch before and after probiotic administration.
RESULTS: In the first set of 2D gels, 26 different proteins with at least 2-fold changes in their expression levels between the pouchitis condition and antibiotic-induced remission were identified. In the second set of analysis, the comparison between mucosal biopsy proteomes in the normal and probiotic-treated pouch resulted in 17 significantly differently expressed proteins. Of these, 8 exhibited the same pattern of deregulation as in the pouchitis/pouch remission group.
CONCLUSION: For the first time, 2D protein maps of mucosal biopsies from patients with ileal pouch-anal anastomosis were provided, and differentially expressed proteins following antibiotic/probiotic treatment were identified.
© 2010 Baishideng. All rights reserved.
Key words: Chronic disease; Pouchitis; Antibiotics; Probiotics; Proteins; Gene expression
Peer reviewer: Dr. Sara K Lindén, Professor, Mucosal Immunobiology and Vaccine Center, Gothenburg University, Box 435, Göteborg, 405 30, Sweden
Turroni S, Vitali B, Candela M, Gionchetti P, Rizzello F, Campieri M, Brigidi P. Antibiotics and probiotics in chronic pouchitis: A comparative proteomic approach. World J Gastroenterol 2010; 16(1): 30-41 Available from: URL: http://www.wjgnet.com/1007-9327/full/v16/i1/30.htm DOI: http://dx.doi.org/10.3748/wjg.v16.i1.30
Total proctocolectomy with ileal J-pouch-anal anastomosis (IPAA) is the surgical treatment of choice for patients with refractory ulcerative colitis (UC) or UC with dysplasia. Although the surgery generally cures UC and has been shown to result in a significant improvement of health-related quality of life, complications can occur after IPAA[1].
The most common long-term complication is pouchitis, an idiopathic inflammatory disease of the ileal reservoir. The reported incidence of pouchitis is variable, largely because of differences in the type and duration of follow-up. However, studies have shown that as many as 15%-46% of patients with UC develop at least 1 episode of pouchitis within 5 years after surgery[2].
Clinically, pouchitis is characterized by variable symptoms, including increased stool frequency and fluidity, abdominal cramping, pelvic discomfort, bleeding, tenesmus, fever and weight loss, and extra-intestinal manifestations in more severe cases[3]. For an unequivocal diagnosis, endoscopic examination and histologic investigation are mandatory[4]. Pouchitis Disease Activity Index (PDAI) is the most commonly used diagnostic instrument and represents an objective and reproducible scoring system for pouchitis[5]. Active pouchitis is defined as a score ≥ 7 and remission is defined as a score < 7.
The etiology and pathophysiology of pouchitis are still poorly understood. However, the fact that pouchitis almost exclusively occurs in patients with underlying UC and that it generally responds to antibacterial therapy suggests a role for the gut microbiota and a genetic predisposition[6].
The disease activity of pouchitis can be defined as remission, mild-moderate or severe based primarily on symptoms. Duration can be classified as acute (< 4 wk) or chronic (≥ 4 wk). Disease pattern can be infrequent (1-2 acute episodes), relapsing (≥ 3 acute episodes) or chronic (a treatment-responsive form requiring maintenance therapy or a treatment-resistant form). Approximately 10%-15% of patients with pouchitis experience a chronic pouchitis, either treatment-responsive or treatment-refractory, and some of them require surgical excision or exclusion of the pouch because of impairment of reservoir function and poor quality of life[7].
Treatment of pouchitis is largely empirical. Broad-spectrum antibiotics have been widely used and represent the mainstay of treatment. Small randomized trials have shown that both metronidazole and ciprofloxacin, alone, sequentially or in combination, are effective in reducing the PDAI score and achieving a significant improvement in clinical symptoms and endoscopic and histologic findings. However, metronidazole is poorly tolerated and treatment with systemically active antibiotics is not ideal from the perspective of the development of antibiotic resistance. In addition, in chronic pouchitis antibiotic-induced remission periods are often short and the condition is complicated by frequent relapses[8].
Recently, several studies have suggested that altering the microbiota in the pouch by administering probiotic bacteria can be effective in maintaining remission and reducing the incidence of flare-ups in chronic pouchitis[9,10]. Moreover, the efficacy of probiotic therapy as prophylaxis to delay the first onset of pouchitis after pouch surgery, has been demonstrated[11,12].
Comparative proteomic analysis represents an effective tool to identify proteins critical for functional pathways in normal cells and phenotype changes that occur during disease development. Since biological and functional output of cells is governed primarily by proteins, the applications of proteomic technologies are beginning to have a profound impact on understanding of the molecular mechanisms underlying several disease processes, which, in turn, will help to reduce disease-related morbidity and mortality. However, despite their extensive use in proteomic profiling of gene expression in various diseases, the applications of such technologies in inflammatory bowel diseases are still in their infancy[13] and, so far, no proteomic study has been reported in IPAA research.
In the present study, we apply 2-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) to define the differential protein displays of mucosal biopsy samples from patients with chronic refractory pouchitis before and after antibiotic treatment. The same proteomic approach has also been applied to identify specific changes in protein expression in the non-inflamed vs probiotic-administered pouch in order to provide a picture of the intestinal mucosa protein modulation by probiotics.
Patients and biopsy collection
Six patients who underwent restorative proctocolectomy with IPAA were recruited for this study and routinely followed up by the Department of Internal Medicine and Gastroenterology, University of Bologna, Polyclinic S. Orsola. Patients were included if they had a chronic refractory pouchitis, defined as no response to at least 4 wk of standard antibiotic therapies (ciprofloxacin 1 g twice daily (bid) or metronidazole 400 mg 3 times daily). They were divided in 2 groups according to PDAI score at study entry and treatment received. In the first group, 3 patients with PDAI ≥ 7 were orally administered with a combination of metronidazole (500 mg bid) and ciprofloxacin (500 mg bid) for 1 mo. The second group, including the other 3 patients with chronic refractory pouchitis but with a total PDAI < 7 at study entry, received VSL#3 (VSL pharmaceuticals Inc., Ft. Lauderdale, FL, USA) 2 packets bid for 3 mo. VSL#3 contains 450 billion viable lyophilized bacteria per packet, comprised of 4 strains of lactobacilli (Lactobacillus acidophilus, L. casei, L. delbrueckii subsp. bulgaricus and L. plantarum), 3 strains of bifidobacteria (Bifidobacterium breve, B. infantis and B. longum) and one strain of Streptococcus thermophilus. Mucosal biopsies were collected during pouch endoscopy before and after antibiotic/probiotic therapy.
All samples were immediately snap frozen in liquid nitrogen. The institutional ethics committee approved all protocols and all enrolled subjects gave their informed consent.
Protein extraction
Frozen mucosal biopsies (about 10-20 mg) were washed in 200 mL of cold low salt washing buffer (3 mmol/L KCl, 1.5 mmol/L KH2PO4, 68 mmol/L NaCl, 9 mmol/L NaH2PO4), with Complete Protease Inhibitor (Roche Molecular Biochemicals, Mannheim, Germany). After centrifugation at 13 000 r/min for 2 min, tissue samples were homogenized in 1 mL of lysis solution (0.11 mol/L DTT, 0.11 mol/L CHAPS, 8 mol/L urea, 2 mol/L thiourea, 35 mmol/L Tris and Complete Protease Inhibitor) using an Ultra-Turrax® homogenizer (IKA Labortechnik, Staufen, Germany). Protein extraction was performed as previously described[14]. Total protein concentration of the cell extract was calculated using the PlusOne 2D Quant Kit™ (GE Healthcare, Uppsala, Sweden). The protein extract preparation was immediately used or aliquoted and frozen at -20℃.
Samples containing 100 mg of protein were diluted to 250 mL with rehydration solution (8 mol/L urea, 2% CHAPS, 10 mmol/L DTT, 2% (v/v) ampholine, pH 3.5-9.5 (GE Healthcare) and trace bromophenol blue) and applied to Immobiline DryStrips (13 cm, pH 3-10, GE Healthcare) for 12 h rehydration at 50 V. Isoelectric focusing was performed using IPGphor apparatus (GE Healthcare) to give a total of 19 kVh. IPG strips were then reduced and alkylated[15] prior to loading onto 15% acrylamide separating gels (20 cm long, 1 mm thickness). Electrophoresis was performed at 250 V for 7 h using Protean Ⅱ xi Cell (Bio-Rad, Hercules, CA, USA). Protein spots were visualized with a MS-compatible silver-staining procedure[16].
Image analysis
Protein patterns in the gels were recorded as digitalized images using a GS-800 imaging densitometer (Bio-Rad). Spot detection, matching and the examination of differentially expressed proteins were performed by PDQuest v6.2 software (Bio-Rad). Three technical replicates were made per patient and condition and formed 1 replicate group with average normalized spot intensities. The comparison was carried out for each patient before and after antibiotic/probiotic therapy. Proteins that showed at least 2 times enhanced/decreased expression were selected for identification along with a few spots that showed a similar expression pattern in all 2D gels.
Protein identification
Protein spots with conserved expression levels throughout the gels in all patients and conditions were identified. Two identification methods were employed: comparison of our reference proteome map with Swiss-2D PAGE (http://www.expasy.ch/ch2d/) and other published 2D proteome patterns[17-21] obtained under very similar experimental conditions, and MALDI-TOF MS analysis. Since both methods provided the same identification result for each spot, we used the gel matching method to identify the differentially expressed proteins in pouchitis/antibiotic-induced remission and normal pouch/probiotic-treated pouch groups. When gel matching produced an unreliable and doubtful identification, because of excessive deviations in pI and Mr values across gels, MALDI-TOF MS was employed.
Protein spots were manually excised from 2D gels, washed and in-gel digested as previously reported[22]. Crude digests were concentrated and desalted using mC18 ZipTips (Millipore, Bedford, MA, USA). Peptide extracts were mixed on the MALDI-TOF target (Applied Biosystems, Foster City, CA, USA) with an equal matrix volume of 5 mg/mL a-cyano-4-hydroxycinnamic acid (Sigma-Aldrich, St. Louis, MO, USA) saturated with 50% acetonitrile/0.2% trifluoroacetic acid, and analyzed using a Voyager-DE Pro Biospectrometry Workstation (Applied Biosystems). All mass spectra were obtained in a reflectron mode, with an accelerating voltage of 20 kV and a delayed extraction of 40 ns. Internal mass calibration with peptides arising from trypsin autoproteolysis was performed. Peptide masses were searched against Swiss-Prot, TrEMBL and NCBI non-redundant protein databases using ProFound (http://prowl.rockefeller.edu/prowl-cgi/profound.exe) and Aldente (http://expasy.org/tools/aldente) programs. Search parameters were set to allow up to one missed tryptic cleavage and a peptide mass tolerance of 50 ppm. Only protein hits with a significant probability score calculated by software and at least 3 matching peptide masses were considered.
Statistical analysis
Statistical analysis of protein expression was performed using the Student's t-test carried out with SigmaStat v3.5 software (Systat Software, Point Richmond, CA, USA). A P value < 0.05 was considered as statistically significant. Bibliometric analysis for co-citation was performed using Bibliosphere Pathway Edition from Genomatix (Genomatix Software, Munich, Germany).
Clinical outcome of antibiotic/probiotic treatment
All the enrolled subjects completed the study. In the first group of patients, after 1 mo of antibiotic therapy, clinical and endoscopic remission was achieved with a significant decrease in both PDAI and median stool frequency (data not shown). In the second group, no episodes of active pouchitis were recorded during the probiotic administration. Both treatments were well tolerated and no side effects were recorded.
Antibiotic administration-related effects on mucosal biopsy proteome in pouchitis
An example of 2D gels obtained from mucosal biopsies in
pouchitis and pouch remission is provided in
Figure 1A.
For each patient, 2D patterns of mucosal biopsies collected before and after antibiotic administration were compared by PDQuest. Because of the high intrinsic variability among individuals, a stringent criterion was applied whereby only those proteins with at least 2 times increased or decreased expression and deregulation in the same way in all patients were considered. Out of 40 differentially expressed protein spots, 26 (65%) were identified, of which 15 were upregulated and 11 downregulated in antibiotic-induced remission of pouchitis (Figure 1A and Table 1). In addition, 6 protein spots with a similar expression pattern in all 2D gels were selected and identified (Figure 1 and Table 2).
The altered proteins were classified in terms of their subcellular location and biological function by information from Swiss-Prot, HPRD (Human Protein Reference Database, http://www.humanproteinpedia.org), and COGs (Cluster of Orthologous Groups of proteins, http://www.ncbi.nlm.nih.gov/COG/) (Figure 2A). The majority of the identified proteins were located in the cytoplasm (38%), mitochondria (27%) and endoplasmic reticulum (11%). Twenty-seven percent of the altered proteins play a key role in post-translational modifications and protein turnover as chaperones, 15% are involved in energy production and conversion, and 11% are related to lipid transport and metabolism.
The results of a histogram data analysis carried out on the spot quantity values determined by PDQuest are displayed in Figure 3 together with representative gel images for each protein spot in each patient and clinical condition. A statistically significant increased expression in pouch remission was detected for tubulin b-2C chain (TUBB), ATP synthase subunit b (ATP5B) and calponin-2 (CNN2) in all patients, whereas calreticulin (CALR), 60 kDa heat shock protein (HSP60), heat shock cognate 71 kDa protein (HSPA8), and intestinal (FABP2) and liver fatty acid-binding proteins (FABP1) expression patterns showed an increase with statistical significance in only 1 or 2 out of the 3 patients enrolled. For ileal lipid binding protein (FABP6) and electron transfer flavoprotein subunit a (ETFA), p values of 0.07 and 0.06, respectively, near the threshold of significance were obtained. Among downregulated protein spots after antibiotic treatment, statistical significance was achieved in all patients for thioredoxin domain-containing protein 5 (TXNDC5), type I cytoskeletal keratin 20 (KRT20) and cathepsin D (CTSD). Pyruvate dehydrogenase E1 component subunit b (PDHB) showed a statistically significant decreased expression in only 1 patient.
Probiotic administration-related effects on mucosal biopsy proteome in non-inflamed pouch
Representative 2D gels obtained from mucosal biopsies in normal pouch and after probiotic therapy are shown in Figure 1B, confirming the protein maps reported in Figure 1A in terms of number, Mr and pI of the spots.
For each of the 3 subjects enrolled, the comparison of the 2D patterns of non-inflamed mucosal biopsies before and after VSL#3 administration was performed by PDQuest as reported above. Seventeen spots, which represented 75% of total proteins recognized as differentially expressed, were identified, of which 7 were upregulated and 10 were downregulated in the probiotic-treated pouch (Figure 1B and Table 1). In addition, it was possible to identify 6 protein spots that showed a similar expression pattern in all 2D gels (Figure 1 and Table 2).
Pie charts representing the subcellular location and the functional distribution of the probiotic administration-altered proteins are reported in Figure 2B. The majority of the identified proteins were in the cytoplasm (41%), mitochondria (35%) and endoplasmic reticulum (12%). The functional classification indicated that 29% play a key role in energy production and conversion, 17% are related to post-translational modifications and protein turnover as chaperones and 12% are involved in carbohydrate transport and metabolism.
The spot quantity values determined by PDQuest are shown in the form of a histogram in Figure 4 together with representative gel images for each protein spot in each subject and condition. A statistically significant increased expression after 3 mo of probiotic administration was detected for HSPA8 and PDHB in all the subjects enrolled. p values of 0.06, near the threshold of significance, were obtained for vinculin (VCL) and phosphoenolpyruvate carboxykinase (PCK2) in 3 and 2 patients, respectively. Among protein spots with downregulated expression levels after VSL#3 therapy, statistical significance was achieved in all patients for KRT20 and in only 1 for TXNDC5.
Bibliometric analysis
On the basis of literature co-citation from NCBI PubMed, a protein-protein network tree using the data-mining program Bibliosphere software was generated. As shown in Figure 5, the network tree was compiled of 28 different proteins forming 2 network clusters. Group 1 consisted of 26 highly interrelated proteins including ATP5B, carbonic anhydrase Ⅰ (CA1) and Ⅱ (CA2), creatine kinase (CKMT1B), a-enolase (ENO1), PCK2, PDHB and triosephosphate isomerase (TPI1), associated with energy, carbohydrate and amino acid metabolism, as well as glycolysis/gluconeogenesis, oxidative phosphorylation and electron transport chain. The second group was formed by 2 linear co-cited proteins, NADH-ubiquinone oxidoreductase 75 kDa subunit (NDUFS1) and cytochrome b-c1 complex subunit 2 (UQCRC2), related to energy production and conversion. The residual 5 detected proteins, S-adenosylmethionine decarboxylase (AMD1), CNN2, tubulin a-1A chain (TUBA1A), TUBB and TXNDC5 were completely disconnected from the network tree.
In this study, we provided for the first time 2D protein maps of mucosal biopsy samples collected during pouch endoscopy in patients who underwent IPAA.
The comparison between mucosal biopsy proteomes in pouchitis and in antibiotic-induced remission enabled the identification of 26 different proteins with at least 2-fold changes in their expression levels. Statistical significance was achieved for ATP5B, CNN2, CTSD, KRT20, TUBB and TXNDC5. In addition, a statistically significant altered expression pattern was obtained for CALR, HSP60, HSPA8, FABP1, FABP2 and PDHB in 1 or 2 of the 3 patients enrolled.
Among the identified mitochondrial proteins, ATP5B, ETFA and PCK2 directly participate in the process of energy production. The decrease of their expression levels in the inflamed pouch suggests the decline of mitochondrial function with pouchitis onset. This assumption is consistent with a previous hypothesis that chronic intestinal inflammation represents an energy-deficiency disease with alterations in the oxidative metabolism of the epithelial cells[23]. Moreover, the low expression of FABP1, FABP2 and FABP6, involved in enhancing the uptake of fatty acids into cells and facilitating their transport to intracellular organelles, could reinforce the speculation that pouchitis-diseased enterocytes do not perform b-oxidation/oxidative phosphorylation owing to a lack of normal supply of fatty acids[24]. Combined with these results, the overexpression of ENO1 found in the inflamed pouch may reflect a shift toward anaerobic glycolysis to overcome the decreased ATP formation by a dysfunctional oxidative phosphorylation[21].
The hypothesis of cellular stress and hypoxic conditions in chronically inflamed tissues is supported by the induction of several chaperone proteins, including 75 (GRP75) and 78 kDa glucose-related proteins (GRP78), TXNDC5, voltage-dependent anion-selective channel protein 1 (VDAC1), CTSD and peptidyl-prolyl cis-trans isomerase A (PPIA)[25-27]. In addition, we detected a statistically significant altered expression pattern for TUBB, KRT20 and CNN2, suggesting changes in cytoskeletal architecture with potential alterations in signal transduction and cellular transcription profiles[27,28].
Next, we compared mucosal biopsy proteomes in the normal pouch before and after probiotic administration and we identified 17 different proteins with significant changes in their expression levels. Interestingly, 8 of the differentially expressed proteins exhibited the same pattern of deregulation as in the pouchitis/pouch remission group. Indeed, both antibiotic and probiotic therapy resulted in downregulation of GRP75, serotransferrin (TF), TXNDC5, KRT20, ENO1 and in upregulation of HSPA8, PCK2 and AMD1, suggesting profound structural and metabolic alterations in enterocytes. In particular, TXNDC5 is a newly identified member of the thioredoxin family of endoplasmic reticulum proteins[29], and it has been proposed as a promising biomarker for cancer diagnosis[30]. Because of its important role in redox regulation[31], the altered expression profile of TXNDC5 in IPAA may be related to the increased oxidative stress with significantly lower plasma concentrations of lipophilic antioxidants and higher free radical activity measured in patients with restorative proctocolectomy compared to normal subjects[32]. Furthermore, for KRT20 and ENO1, widely applied as diagnostic markers for colon adenocarcinomas and many other tumors[33,34], as well as for PCK2 and AMD1 a differential protein profile in inflammatory bowel disease has been already reported[21,35,36].
In addition to these results, in the VSL#3-treated pouch we found a statistically significant upregulation of VCL and an altered expression pattern for TUBA1A, supporting the assumption of a positive modulation exerted by probiotics at cytoskeleton level for cell morphology and integrity[37,38]. In addition, the dysregulated expression levels of NDUFS1, CKMT1B, UQCRC2, PDHB, CA2 and TPI1, directly involved in energy metabolism, strengthen the hypothesis of significant changes in the metabolic profiles of the host associated with probiotic administration[39,40]. Nonetheless, although the manipulation of the ubiquitin/proteasome pathway and the ability to intervene with the complex host system of detoxification of potentially harmful xenobiotics and endobiotic compounds may account for some of the cytoprotective effects of probiotics[37,41,42], we did not find any significant change in glutathione S-transferase P (GSTP1) and ubiquitin-conjugating enzyme E2 N (UBE2N) protein expression levels.
The bibliometric data analysis including all the 33 differentially regulated proteins from the pouchitis/pouch remission and non-inflamed/probiotic-treated pouch group comparison generated a complex network with 26 highly interrelated proteins. As expected, the majority of clustered proteins were associated with glycolysis/gluconeogenesis, oxidative phosphorylation and electron transfer chain pathways.
In conclusion, the identified proteins, both upregulated and downregulated, may be involved in pouchitis pathophysiology and participate in disease onset or in maintenance of the non-inflamed pouch.
Restorative proctocolectomy with ileal pouch-anal anastomosis (IPAA) is the procedure of choice for complicated ulcerative colitis. In the long-term, up to 50% of patients develop pouchitis, an idiopathic inflammatory disease of the ileal reservoir. The management of pouchitis is largely empirical and only few small placebo-controlled clinical trials have been conducted. Although antibiotics represent the mainstay of treatment, probiotics have recently gained more attention as an effective therapeutic option for pouchitis management.
Research frontiers
The etiology and pathophysiology of pouchitis are still not entirely clear but the bulk of the evidence points towards an abnormal mucosal immune response to altered microbiota patterns. By investigating the dynamic nature of protein expression, cellular and subcellular distribution, posttranslational modifications and protein-protein interaction networks, proteomic technologies could play a major role in unraveling the mystery of immunopathogenic mechanisms of pouchitis and in discovering novel biomarkers for disease activity, diagnosis and prognosis.
Innovations and breakthroughs
The current study is the first proteomic study to be reported in IPAA research. The authors provided the 2D protein maps of mucosal biopsy samples collected during pouch endoscopy in patients with chronic refractory pouchitis. The changes in the protein expression profiles following antibiotic or probiotic treatment were characterized.
The identified proteins, upregulated or downregulated following antibiotic/probiotic treatment, may be involved in pouchitis pathophysiology and participate in disease onset or in maintenance of the non-inflamed pouch. Future work will be focused in validating the list of proteins identified in larger patient cohorts.
Peer review
The results are well described and interesting, although the number of patients is a bit on the small side. Even though this manuscript does not give a clear understanding to the mechanistic differences, the results may aid other scientists in making a follow-up study.
1 Baumgart DC, Sandborn WJ. Inflammatory bowel disease: clinical aspects and established and evolving therapies. Lancet 2007; 369: 1641-1657 PubMed DOI
2 McLaughlin SD, Clark SK, Tekkis PP, Ciclitira PJ, Nicholls RJ. Review article: restorative proctocolectomy, indications, management of complications and follow-up--a guide for gastroenterologists. Aliment Pharmacol Ther 2008; 27: 895-909 PubMed DOI
3 Madden MV, Farthing MJ, Nicholls RJ. Inflammation in ileal reservoirs: 'pouchitis'. Gut 1990; 31: 247-249 PubMed DOI
4 Shen B, Achkar JP, Lashner BA, Ormsby AH, Remzi FH, Bevins CL, Brzezinski A, Petras RE, Fazio VW. Endoscopic and histologic evaluation together with symptom assessment are required to diagnose pouchitis. Gastroenterology 2001; 121: 261-267 PubMed DOI
5 Sandborn WJ, Tremaine WJ, Batts KP, Pemberton JH, Phillips SF. Pouchitis after ileal pouch-anal anastomosis: a Pouchitis Disease Activity Index. Mayo Clin Proc 1994; 69: 409-415 PubMed
6 Shen B. Diagnosis and treatment of patients with pouchitis. Drugs 2003; 63: 453-461 PubMed DOI
7 Sandborn WJ. Pouchitis: definition, risk factors, frequency, natural history, classification, and public perspectives. In: McLeod RS, Martin F, Sutherland LR, Wallace JL, Williams CN, editors. Trends in Inflammatory Bowel Disease 1996. Lancaster: Kluwer Academic Publishers, 1997: 51-63
8 Maser EA, Present DH. Pouch-ouch. Curr Opin Gastroenterol 2008; 24: 70-74 PubMed DOI
9 Gionchetti P, Rizzello F, Venturi A, Brigidi P, Matteuzzi D, Bazzocchi G, Poggioli G, Miglioli M, Campieri M. Oral bacteriotherapy as maintenance treatment in patients with chronic pouchitis: a double-blind, placebo-controlled trial. Gastroenterology 2000; 119: 305-309 PubMed DOI
10 Mimura T, Rizzello F, Helwig U, Poggioli G, Schreiber S, Talbot IC, Nicholls RJ, Gionchetti P, Campieri M, Kamm MA. Once daily high dose probiotic therapy (VSL#3) for maintaining remission in recurrent or refractory pouchitis. Gut 2004; 53: 108-114 PubMed DOI
11 Gionchetti P, Rizzello F, Helwig U, Venturi A, Lammers KM, Brigidi P, Vitali B, Poggioli G, Miglioli M, Campieri M. Prophylaxis of pouchitis onset with probiotic therapy: a double-blind, placebo-controlled trial. Gastroenterology 2003; 124: 1202-1209 PubMed DOI
12 Gosselink MP, Schouten WR, van Lieshout LM, Hop WC, Laman JD, Ruseler-van Embden JG. Delay of the first onset of pouchitis by oral intake of the probiotic strain Lactobacillus rhamnosus GG. Dis Colon Rectum 2004; 47: 876-884 PubMed DOI
13 Alex P, Gucek M, Li X. Applications of proteomics in the study of inflammatory bowel diseases: Current status and future directions with available technologies. Inflamm Bowel Dis 2009; 15: 616-629 PubMed DOI
14 Vitali B, Wasinger V, Brigidi P, Guilhaus M. A proteomic view of Bifidobacterium infantis generated by multi-dimensional chromatography coupled with tandem mass spectrometry. Proteomics 2005; 5: 1859-1867 PubMed DOI
15 Görg A, Postel W, Günther S. The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 1988; 9: 531-546 PubMed DOI
16 Shevchenko A, Wilm M, Vorm O, Mann M. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal Chem 1996; 68: 850-858 PubMed DOI
17 Melis R, White R. Characterization of colonic polyps by two-dimensional gel electrophoresis. Electrophoresis 1999; 20: 1055-1064 PubMed DOI
18 Mori Y, Kondo T, Yamada T, Tsuchida A, Aoki T, Hirohashi S. Two-dimensional electrophoresis database of fluorescence-labeled proteins of colon cancer cells. J Chromatogr B Analyt Technol Biomed Life Sci 2005; 823: 82-97 PubMed DOI
19 Lenaerts K, Bouwman FG, Lamers WH, Renes J, Mariman EC. Comparative proteomic analysis of cell lines and scrapings of the human intestinal epithelium. BMC Genomics 2007; 8: 91 PubMed DOI
20 Li M, Xiao ZQ, Chen ZC, Li JL, Li C, Zhang PF, Li MY. Proteomic analysis of the aging-related proteins in human normal colon epithelial tissue. J Biochem Mol Biol 2007; 40: 72-81 PubMed
21 Shkoda A, Werner T, Daniel H, Gunckel M, Rogler G, Haller D. Differential protein expression profile in the intestinal epithelium from patients with inflammatory bowel disease. J Proteome Res 2007; 6: 1114-1125 PubMed DOI
22 Shevchenko A, Tomas H, Havlis J, Olsen JV, Mann M. In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nat Protoc 2006; 1: 2856-2860 PubMed DOI
23 Roediger WE. The colonic epithelium in ulcerative colitis: an energy-deficiency disease? Lancet 1980; 2: 712-715 PubMed DOI
24 Esteve M, Navarro E, Klaassen J, Abad-Lacruz A, González-Huix F, Cabré E, Ramos E, Condom E, Fernández-Bañares F, Pastor C, Humbert P, Martí-Ragué J, Gassull MA. Plasma and mucosal fatty acid pattern in colectomized ulcerative colitis patients. Dig Dis Sci 1998; 43: 1071-1078 PubMed DOI
25 Abu-Hamad S, Sivan S, Shoshan-Barmatz V. The expression level of the voltage-dependent anion channel controls life and death of the cell. Proc Natl Acad Sci USA 2006; 103: 5787-5792 PubMed DOI
26 Menzel K, Hausmann M, Obermeier F, Schreiter K, Dunger N, Bataille F, Falk W, Scholmerich J, Herfarth H, Rogler G. Cathepsins B, L and D in inflammatory bowel disease macrophages and potential therapeutic effects of cathepsin inhibition in vivo. Clin Exp Immunol 2006; 146: 169-180 PubMed DOI
27 Werner T, Haller D. Intestinal epithelial cell signalling and chronic inflammation: From the proteome to specific molecular mechanisms. Mutat Res 2007; 622: 42-57 PubMed DOI
28 Keshavarzian A, Banan A, Farhadi A, Komanduri S, Mutlu E, Zhang Y, Fields JZ. Increases in free radicals and cytoskeletal protein oxidation and nitration in the colon of patients with inflammatory bowel disease. Gut 2003; 52: 720-728 PubMed DOI
29 Knoblach B, Keller BO, Groenendyk J, Aldred S, Zheng J, Lemire BD, Li L, Michalak M. ERp19 and ERp46, new members of the thioredoxin family of endoplasmic reticulum proteins. Mol Cell Proteomics 2003; 2: 1104-1119 PubMed DOI
30 Wang Y, Ma Y, Lü B, Xu E, Huang Q, Lai M. Differential expression of mimecan and thioredoxin domain-containing protein 5 in colorectal adenoma and cancer: a proteomic study. Exp Biol Med (Maywood) 2007; 232: 1152-1159 PubMed DOI
31 Edman JC, Ellis L, Blacher RW, Roth RA, Rutter WJ. Sequence of protein disulphide isomerase and implications of its relationship to thioredoxin. Nature 1985; 317: 267-270 PubMed DOI
32 El Muhtaseb MS, Talwar D, Duncan A, St J O'reilly D, McKee RF, Anderson JH, Foulis A, Finlay IG. Free radical activity and lipid soluble anti-oxidant vitamin status in patients with long-term ileal pouch-anal anastomosis. Colorectal Dis 2009; 11: 67-72 PubMed DOI
33 Durany N, Joseph J, Campo E, Molina R, Carreras J. Phosphoglycerate mutase, 2,3-bisphosphoglycerate phosphatase and enolase activity and isoenzymes in lung, colon and liver carcinomas. Br J Cancer 1997; 75: 969-977 PubMed
34 Stenling R, Lindberg J, Rutegård J, Palmqvist R. Altered expression of CK7 and CK20 in preneoplastic and neoplastic lesions in ulcerative colitis. APMIS 2007; 115: 1219-1226 PubMed DOI
35 Obayashi M, Matsui-Yuasa I, Matsumoto T, Kitano A, Kobayashi K, Otani S. Polyamine metabolism in colonic mucosa from patients with ulcerative colitis. Am J Gastroenterol 1992; 87: 736-740 PubMed
36 Vermeulen N, Arijs I, Joossens S, Vermeire S, Clerens S, Van den Bergh K, Michiels G, Arckens L, Schuit F, Van Lommel L, Rutgeerts P, Bossuyt X. Anti-alpha-enolase antibodies in patients with inflammatory bowel disease. Clin Chem 2008; 54: 534-541 PubMed DOI
37 Yang F, Wang J, Li X, Ying T, Qiao S, Li D, Wu G. 2-DE and MS analysis of interactions between Lactobacillus fermentum I5007 and intestinal epithelial cells. Electrophoresis 2007; 28: 4330-4339 PubMed DOI
38 Seth A, Yan F, Polk DB, Rao RK. Probiotics ameliorate the hydrogen peroxide-induced epithelial barrier disruption by a PKC- and MAP kinase-dependent mechanism. Am J Physiol Gastrointest Liver Physiol 2008; 294: G1060-G1069 PubMed DOI
39 Nerstedt A, Nilsson EC, Ohlson K, Håkansson J, Thomas Svensson L, Löwenadler B, Svensson UK, Mahlapuu M. Administration of Lactobacillus evokes coordinated changes in the intestinal expression profile of genes regulating energy homeostasis and immune phenotype in mice. Br J Nutr 2007; 97: 1117-1127 PubMed DOI
40 Martin FP, Wang Y, Sprenger N, Yap IK, Lundstedt T, Lek P, Rezzi S, Ramadan Z, van Bladeren P, Fay LB, Kochhar S, Lindon JC, Holmes E, Nicholson JK. Probiotic modulation of symbiotic gut microbial-host metabolic interactions in a humanized microbiome mouse model. Mol Syst Biol 2008; 4: 157 PubMed DOI
41 Tien MT, Girardin SE, Regnault B, Le Bourhis L, Dillies MA, Coppée JY, Bourdet-Sicard R, Sansonetti PJ, Pédron T. Anti-inflammatory effect of Lactobacillus casei on Shigella-infected human intestinal epithelial cells. J Immunol 2006; 176: 1228-1237 PubMed
42 Sauer J, Richter KK, Pool-Zobel BL. Products formed during fermentation of the prebiotic inulin with human gut flora enhance expression of biotransformation genes in human primary colon cells. Br J Nutr 2007; 97: 928-937 PubMed DOI
S- Editor Tian L L- Editor Cant MR E- Editor Zheng XM


text:
Thousands of ringtones on Audiko.net! You can just download a free Sina Vodjani ringtones for your iPhone or mobile. Cut your favorite moments from your favorite audio track - Ave, Sthaight to the heart, Straight To The Heart, or any other track. You are welcome to obtain your tune both in the MP3 and m4a formats, compatible with both mobile phones and iPhones. Just select a track, and choose free Sina Vodjani ringtones download option.
Download Sina Vodjani Ringtones
Top free ringtones
|Straight To The Heart
|Sacred Buddah
|Sacred Buddha
Sina Vodjani
Sina Vodjani


text:
View is a collection of projects by the students and faculty of Harvard University's Graduate School of Design that were undertaken during the current academic year. Organized alphabetically, the book juxtaposes contributions from a diversity of design fields—including architecture, landscape architecture, and urban planning and design. The combination of design projects and scholarly research provides an impressive panorama of interests and investigations. Each submission is a form of speculation, in most instances supported by long periods of sustained study.
The random adjacenies and proximities of this publication will, I hope, help the reader to construct her/his own domains of cross-disciplinary activity, building on those already developed, whether implicitly or explicitly, within each of its pages. View, if used in this way, will become a catalyst for new forms of imagination in response to our changing concerns and care for design and the environment.
Mohsen Mostafavi
Alexander and Victoria Wiley Professor of Design
Harvard University Graduate School of Design
No Comments for "A View on Harvard GSD, Vol.3"


text:
Currency Displayed in
Hover over image to zoom
Copyright 2016 Moviestore Inc.. All Rights Reserved. Sitemap |
Bigcommerce Premium Themes by PSDCenter
All prices are in USD.
Compare Now
Click the button below to add the STACY FERGUSON SEXY ON STAGE PERFORMING BLACK EYED PEAS POSTER AND PHOTO 287534 to your wish list.


text:
+49 89 127695-100
Monday to Friday, from 8:00 am till 8:00 pm CET and Saturday, from 9:00 am till 6:00 pm CET (with the exception of German public holidays)
For more information, please refer to our customer care page.
Nina Ricci strikes the perfect balance of classic femininity and romantic soul with just the right touch of modernity.
It's been all change at Nina Ricci recently. During his five-year tenure at Nina Ricci, former creative director Peter Copping was successful in his work that was as confident as it was soft, "typically French – sexy but not vulgar", and in line with modern-day femininity. We waited with bated breath to see how Guillaume Henry would fill his shoes… we weren't wrong to be pretty damn excited.
Nina Ricci's latest looks are as easy to wear as they are elegant and irresistible. We're smitten with each of the little lace numbers, pleated pieces and that inherently luxe velvet. Demure cuts are tempered exquisitely by dainty detailing, making for something light, ladylike and in altogether rather sugar-sweet.
19 products
19 products


text:
Meal Type
Featured Videos
Featured Content
Featured Brand Sites
Product By Category
Tools & Info
Cooking Tips
Healthy Living
Your Kids
food&family Magazine
4 servings, 1 cup each
Read MoreRead Less
Tap or click steps to mark as complete
Prepare as directed, adding 1 pkg. (6 oz.) OSCAR MAYER CARVING BOARD Flame Grilled Chicken Breast Strips, chopped, to prepared Dinner along with the tomatoes and basil.
For added color and texture, serve with a mixed green salad tossed with your favorite KRAFT Lite Dressing.
* Nutrition information is estimated based on the ingredients and cooking instructions as described in each recipe and is intended to be used for informational purposes only. Please note that nutrition details may vary based on methods of preparation, origin and freshness of ingredients used.
Don't get confused! The "Old World Italian Sauce" refers to the FLAVOR of the Homestyle Mac and Cheese -- they have Four Cheese, Cheddar and Old World Italian. You DON'T add Italian flavored tomato sauce! I'm looking forward to trying this recipe!
Bought a full sized tomato, used only half. Used a 1/2 cup of the Italian Sauce (since the recipe didn't say) and I'd say it was borderline on TOO much tomato.
My family enjoyed this recipe tonight. Thank you Kraft!


text:
Click on a thumbnail to go to Google Books.
Duck & Goose Go to the Beach
by Tad Hills
No current Talk conversations about this book.
A story that promotes crossing boundaries and limits! A great story about it being "okay" to push yourself past your comfort zone.
Another outing for the two charming feathered friends. Fans of the other books will certainly enjoy this one.
This book was about two friends that go to the beach. It was fantasy, because animals were talking. They get tired of the meadow they are in, and so they decide to visit the beach. They spend some time there, but end up not liking it. So they go back to the meadow, and are happy again.
Duck and Goose go the beach in this adorable children's book. Duck wants to go the the beach, but unfortunately, Goose does not want to go the beach. But, eventually, Goose gives in. They go on several adventures and see the biggest tree they have ever seen and the biggest hill. On that hill, they finally see the OCEAN!! Once they finally get to the beach, duck decides that he doesn't like the beach. What?! Duck was the one that wanted to go to the beach to begin with. Sure enough, Goose decides that he LOVES the beach! This short book goes through the adventures that Duck and Goose have at the beach and the kind friendship that the two of them share.
Genre: Fantasy
This book is about friendship. It is about a duck and a goose who want to go on an adventure. Goose doesn't seem the point of it and doesn't want to go. They then take a hike to the beach and they book love the ocean, sand, etc. This is a good book for young children because maybe they have a best friend and they enjoy going on adventure or doing something with that friend. No matter what you choose to do, more than likely everybody will be happy in the end. Goose wasn't thrilled to go an adventure but when he saw how happy duck was Goose didn't think it was that bad after all.
References to this work on external resources.
Wikipedia in English
Amazon.com Product Description (ISBN 0385372353, Hardcover)In this delightful follow-up to the New York Times bestselling Duck & Goose and Duck, Duck, Goose, Duck wants to go on an adventure. Goose doesn't. He doesn't see the point. After all, why would they go anywhere when they're happy right where they are? But then Goose sees the ocean and loves it. Who doesn't? Well, Duck, for one!
(retrieved from Amazon Thu, 12 Mar 2015 18:14:26 -0400)
"Duck wants to go on an adventure. Goose doesn't. He doesn't see the point. After all, why would they go anywhere when they're happy right where they are? But then Goose sees the ocean and loves it. Who doesn't? Well, Duck, for one"--
Amazon Kindle (1 edition)
Audible (0 editions)
CD Audiobook (0 editions)
Project Gutenberg (0 editions)
Google Books — Loading...
Is this you?
Become a LibraryThing Author.


text:
What are you doing this weekend? Just the usual right? Getting groceries, paying bills, catching up with "Top Chef"... But if you're Quentin Tarantino, and you find out that Bong Joon-ho will be appearing at the Busan International Film Festival, well you can just get on a plane and drop by. And tha...Read More »
While The Weinstein Company outlines exactly what manner of compromised cut they will inflict upon Bong Joon-ho and his upcoming "Snowpiercer," we can't blame the director for wanting to move swiftly on to his next project and away from frustrating negotiations. Luckily, the talented filmmaker is fl...Read More »
Back in August some news surfaced to the horror of Bong Joon-ho fans everywhere; that The Weinstein Company were looking to slice and dice his upcoming "Snowpiercer" by upwards of twenty minutes. While many collectively freaked, the director himself at first remained diplomatic on the matter, callin...Read More »
As the fate is still yet to be determined as to which version of Bong Joon-ho's "Snowpiercer" we'll get stateside, France is gearing up to get the movie next month in its original form, and thus the promo gears are turning abroad. And today brings with it a couple more trailers for the movie.Read More »
Jumping to conclusions before the facts are in is par for the course these days. As is the blind leap to vilify Harvey Weinstein over anything mildly controversial that he has even dared look sideways at (though yes, his track record is poor, but generally the dude knows what he's doing), but at the...Read More »
So far, the reviews for dystopian thriller "Snowpiercer" are raves. But that doesn't mean that Harvey Weinstein won't tinker with the Bong Joon-ho film before releasing it to the territories he controls, which are most of the English-speaking world. Australia trade reporter Don Groves reports that W...Read More »
While we continue to wait for a U.S. release date for Bong Joon-ho's post-apocalyptic "Snowpiercer," it's smashing records at home. It reached 1 million viewers in less than two days in release, breaking a previous record set by the director's "The Host," and becoming the first film to do so in Sout...Read More »
To date, we've seen two international trailers, a featurette, an R-rated animated prequel, and even a handful of positive reviews for Joon-ho Bong's science fiction film, "Snowpiercer." Today brings us another glimpse at the highly-anticipated film in the form of a third international trailer, yet t...Read More »
The first reviews have landed for Bong Joon-ho's dystopian sci-fi entry "Snowpiercer," starring a sprawling cast headed by Tilda Swinton, Chris Evans, Song Kang-ho, Jamie Bell and Octavia Spencer. Variety calls the film "visually stunning and richly satisfying," while Twitch writes that the film's s...Read More »
While The Weinstein Company continues to keep Bong Joon-ho's "Snowpiercer" on a still undetermined place on their future slate, the movie is gearing up to open in South Korea. We've already seen a good handful of teasers, pics and previews of the movie, but finally some folks seen the film in its en...Read More »


text:
LeBron and Heat Muscle Through
MIAMI—Miami Heat coach Erik Spoelstra could sense that it was warmer than usual at AmericanAirlines Arena on Tuesday night. It might have been the pregame pyrotechnics (lots of fire) or the towel-waving crowd (lots of bodies) or simply the thermostat (lots of degrees), but it felt hot for Game 4 of the NBA Finals.
There are convenient metaphors here, about pressure and sticky situations and the friction created by two teams vying for a championship. But forget all that—it was just plain hot, and the conditions had real-world repercussions. The most obvious example was LeBron James crawling around on all fours in the fourth quarter, the victim of leg cramps.
James has been cementing his status as the greatest player of his generation throughout this enthralling series with the Oklahoma City Thunder, and he probably left no doubt after he and the Heat willed themselves to a 104-98 victory here that pushed them to the cusp of a title.
The Daily Fix
James, a three-time MVP in search of his first championship, returned to the game just long enough to sink a late 3-pointer that gave Miami the lead for good. The Heat took a 3-1 lead in the best-of-seven series and will have a chance to close things out back at home Thursday night in Game 5.
"Of course it's there to think about," said James, who finished with 26 points, 12 assists and nine rebounds—just one shy of a triple-double. "But I'm not taking our preparation for Game 5 for granted. We're going to prepare the same way we've been preparing all series."
The Heat survived a tour de force from Thunder guard Russell Westbrook, who scored 43 points on 20-of-32 shooting. He also grabbed seven rebounds to go along with five assists, keeping his team close for long stretches by contorting his body for acrobatic layups and off-balance jumpers.
But a consistent problem for the Thunder has been inexperience, which has revealed itself at inopportune times—fourth quarters, for example. Considering how well Westbrook played, it seemed almost unfortunate that he was the most blatant culprit this time around. With the shot clock about to expire late in the fourth quarter and the Thunder trailing by just three points, Westbrook made the inexplicable decision to foul the Heat's Mario Chalmers. Chalmers stepped to the free-throw line with 13.8 seconds left and calmly sank both foul shots to seal the win.
"Just miscommunication on my part," Westbrook said. "Nothing I can do about it now."
As coach Scott Brooks put it, the Thunder still have "one more crack" at sending the series back to Oklahoma City. But things look pretty bleak for his team. After scoring 28 points in the loss, Kevin Durant spent much of his postgame press conference resting his face on his palm. He kept talking about how he and his teammates need to keep "believing" they can win, but his body language suggested otherwise.
And then there was power forward Kendrick Perkins, who groused about Brooks's decision to abandon the lineup—featuring Perkins, of course—that built a 17-point lead in the first quarter. Brooks opted to play small-ball against the Heat for the remainder of the game.
"I just don't understand why we start out the first quarter the way we did, with the lineup that we had, and all of a sudden we change and adjust to what they had going on," Perkins said. "So, they won the last three quarters, and that's what happened."
This is Oklahoma City's maiden trip to the finals, and all of the confidence and youthful exuberance it carried into the series has evaporated. The Heat can relate. One year ago, James and his teammates had their own meltdown in the finals against the Dallas Mavericks, a team that had a knack for making the big play at the right time and wound up winning the title. Heat center Chris Bosh said the team grew from that experience.
"We were the ones who had the lead last year and couldn't capitalize," he said. "We really learned that we have to play through it until the end."
Against the Thunder, the difference hasn't been the gap between LeBron James and Kevin Durant, or between Dwyane Wade and Russell Westbrook. In many ways, this series has been defined by each team's supporting cast, for better or worse. On Thursday, it was the Heat's Chalmers—a role player unfazed by pressure—who scored 25 points. The Thunder's James Harden, meantime, looked confused at times, downright lost at others. He finished with eight points, five fouls and four turnovers.
For James, one more win would silence many critics who have questioned his mettle in championship moments. He said his legs felt zapped in the fourth quarter, and he was on the bench in the closing seconds. But he contributed when he could, however he could, and he expected his teammates to do the same. It was enough.
Write to Scott Cacciola at firstname.lastname@example.com


text:
Sign in now to view, license and download this image or contact us to see this image without a watermark. Want to embed this image on a non-commercial website or blog? Learn more.
Similar imagesView all
- Cloud - Sky,
- Color Image,
- Day,
- Distant,
- High Angle View,
- Horizon Over Water,
- Horizontal,
- Incidental People,
- Island,
- Mayreau Island,
- Nature,
- Nautical Vessel,
- On The Move,
- Outdoors,
- Photography,
- Sailboat,
- Saint Vincent And The Grenadines,
- Scenics,
- Sea,
- Sky,
- Transportation,
- Travel Destinations,
- Turquoise Colored,
- Yacht,
Sailing Yachts Stock PhotoCloud - Sky,Color Image,Day,Distant,High Angle View,Horizon Over Water,Horizontal,Incidental People,Island,Mayreau Island,Nature,Nautical Vessel,On The Move,Outdoors,Photography,Sailboat,Saint Vincent And The Grenadines,Scenics,Sea,Sky,Transportation,Travel Destinations,Turquoise Colored,YachtPhotographer Adina TovyCollection: Lonely Planet Images Adina TovyView of sailing yachts on Mayreau Island.