text:
Hi everyone
So I'm a pretty big noob to shifting and gears since most of my bikes have been SS but now I am having a shifting issue. The bike is only a couple months old so I'm sure it's probably due for a little tweaking...
Anyway, the rear is indexed shifting, and I find when Im switching to a couple, but not all of the sprockets, that I have to slightly adjust my shifter up front to keep it quiet. It's just a minor inconvenience but didn't always do this.
I can't figure out if this is a front or rear derailleur issue or both. Is this a common issue for indexed shifting?
would appreciate any input!


text:
Our Second Fundraising Campaign
Dear Friend—
Hope you're doing well!
The Shakespearean Jazz Showhas had quite an exciting year! Since we've last been in touch, our ensemble presented at the inauguration of Emerson... Read more
Our Performances in New Orleans Went Very Well!
I am proud to report that after a lot of hard work (and a rigorous 3-day rehearsal schedule) our performances in New Orleans went very well. We had packed... Read more
Opening Next Week in NOLA
Hello Friends,
Greetings from New Orleans!
On Sunday the cast convenes in New Orleans from all over the country to do The Shakespearean Jazz Show at the New Orleans Shakespeare... Read more
Kickstarter Concluding Today
Hello Friends,
Today at 11pm EDT we shall conclude our fundraising campaign for The Shakespearean Jazz Show's summer production in New Orleans.
This Kickstarter campaign has had quite an exhilarating ride:
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A Video Thank You from the Team
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Just wanted to share this video of The Shakespearean Jazz Show team saying "thank you" from all over the country. We still have 11 days left on our... Read more
Thank You!
We did it! We reached our goal in less than a week. With 14 days to spare!
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We've Reached our Halfway Goal
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Within 24 hours, we've raised $2,500—half of our intended goal. What a remarkable achievement! Thank you. Thank you. Thank you. None of this would be possible without your... Read more

subset: commoncrawl


text:
Having a problem with MS Access 2007 refusing to display a form in design mode. I can see the code for the form (if I go through the event properties of a button that displays the form), but I cannot see the form laid out as a GUI. And, when I try and invoke this form from the application's main window, it does not display and locks up the whole GUI.
Now, I tried putting debug before and after the display of this form, and the debug does get invoked.
Anyone have any ideas? I tried looking at the Trust Center, and I set "Enable all Macros", but that didn't seem to matter.


text:
|Publication number
|Publication type
|Application number
|PCT number
|Publication date
|30 Dec 2003
|Filing date
|13 Jul 1998
|Priority date
|11 Jul 1997
|Fee status
|Also published as
|Publication number
|Günter Schmidt, Andrew Hugin Thompson
|Original Assignee
|Xzillion Gmbh & Co.
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (8), Non-Patent Citations (3), Classifications (10), Legal Events (9)
|External Links: USPTO, USPTO Assignment, Espacenet
The present invention concerns a method for categorising nucleic acid. In particular, the invention concerns a method for sorting nucleic acid, which method permits reduction in the complexity of a nucleic acid population of approximately one order of magnitude, or more. The invention also relates to a kit for carrying out the above method.
Analysis of nucleic acids is fundamental to much of modem molecular biology. A particular feature of nucleic acids derived from living organism is that they are almost invariably complex populations of sequences present in widely varying quantities. In order to characterise these populations of nucleic acids it is usual to attempt to reduce the complexity of the population of nucleic acids in some way. Traditionally the approach has been to clone complex nucleic acid molecules into vectors to allow them to be isolated and either sub-cloned further or analysed directly. Cloning requires the use of biological hosts and these are often difficult to use and require a great deal of specialist knowledge for the cloning procedures to be successful. The traditional processes of cloning to generate libraries of sequences are also only partially automatable.
A problem which cloning does not address is how to isolate sequences which are present only at low copies in backgrounds of sequences present at high copy numbers. Various techniques have been developed to 'normalise' complex nucleic acid populations prior to cloning in order to increase the quantities of sequences at low copy numbers relative to those at high copy numbers. Subtractive hybridisation methods have been used to try and normalise cDNA populations.
PCT/GB93/01452 describes methods of molecular sorting which uses restriction endonucleases that generate ambiguous sticky-ends in the nucleic acid sanple to be sorted. Adapters are designed with sticky ends complementary to a single sticky-end sequence or a subset of the these ambiguous sticky ends such that the individual sticky end or subset thereof is coupled to a distinct sequence in the double stranded region of the adapter. This allows subsets of the adaptored nucleic acid to be amplified using specific primers corresponding to sequences within the adapter which in turn relate to the sequence of the sticky end of the adapter. U.S. Pat. No. 5,508,169 (issued Nov. 7, 1995) describes methods very similar to those disclosed in PCT/GB93/01452.
A problem with the above method is that the nucleic acids can be sorted only according to the sequence present on the sticky-ends of the nucleic acid. The sticky-end sequence is of limited length, as determined by the choice of restriction enzyme, thus the basis for sorting is limited.
It is an object of the present invention to provide a method which overcomes the above problems, and provides a wider basis on which sorting of nucleic acid populations can be carried out, not limited by the sticky-end sequence. It is also an object of this invention to provide methods to reduce the complexity of nucleic acid populations by allowing them to be sorted into sub-populations without cloning and to permit normalisation of these populations. This invention describes methods of sorting nucleic acid molecules that have a variety of applications including gene expression profiling, preparation of templates for sequencing, linkage analysis, etc. This invention provides methods of generating sorted libraries. In many applications it is preferable that these sorted nucleic acids be captured on a solid phase support.
Accordingly, the present invention provides a method for categorising nucleic acid, which method comprises producing a nucleic acid population by action of an endonuclease on double-stranded nucleic acid, such that each nucleic acid in the nucleic acid population has a double-stranded portion, contacting the nucleic acid population with one or more oligonucleotide sequences, and isolating nucleic acid which correctly hybridises to an oligonucleotide sequence, wherein each oligonucleotide sequence has a pre-determined recognition sequence, the nucleic acid being categorised by its ability to correctly hybridise to oligonucleotide sequences having the recognition sequence, the recognition sequence being situated such that it recognises a sequence in the double-stranded portion of the nucleic acid, one or more different recognition sequences being represented in the oligonucleotide sequences.
The present invention also provides kit for categorising a nucleic acid, comprising one or more adaptors and one or more sets of oligonucleotide sequences, wherein the adaptors comprise nucleic acid having a double-stranded primer portion of a known sequence and a single-stranded portion of a pre-determined length, either each single-stranded portion of each nucleic acid in the adaptors having the same pre-determined sequence or all possible sequences of the single-stranded portion being represented in the adaptors, and wherein each oligonucleotide sequence comprises a first sequence, a second sequence attached to the first sequence and a third sequence attached to the second sequence, in which the first sequence is complementary to the sequence of the primer portion of the adaptor, the second sequence is the same sequence as the single-stranded portion of the adaptors or all possible second sequences of the same length as the single-stranded portion of the adaptors are represented within the set of oligonucleotides, and the third sequence comprises a pre-determined recognition sequence.
The invention will now be described in further detail by way of example only, with reference to the accompanying drawings, in which:
FIG. 1 shows a schematic of the treatment of a genomic DNA clone with a frequent cutting restriction endonuclease, such as Sau3A1, followed by ligation of adaptors to restriction fragments bearing specific primer sequences—all fragments are dealt with simultaneously, but for simplicity only one is shown;
FIG. 2 shows a schematic of an amplification step, following the steps of FIG. 1, in which fragments are amplified by PCR using adaptor primers;
FIG. 3 shows a step following the step of FIG. 2, in which amplified fragments are subdivided into 10, wells, each well being identified by a pair of primers used to sort added molecules, each well initially containing one of the pair of primers, there being 4 primers each with one base probe sequence and each well having 1 of 10 possible pairs generated by a combination of the four primers, the second primer being added after one cycle of synthesis of the first;
FIG. 4 shows a schematic of a differential amplification step, following the step of FIG. 3, in which the contents of a well containing a primer terminated with AC followed by a probe terminated by AG is amplified and then one cycle of synthesis is performed with the first primer and double strands captured with avidinated beads;
FIG. 5 shows a schematic of steps subsequent to those of FIG. 4, in which the non-immobilised strand is melted off and washed away and the reaction residue polymerised, a second primer then being added and a second cycle of synthesis performed; and
FIGS. 6A and 6B show a schematic of steps subsequent to those of FIG. 5, in which the non-immobilised strand is melted off and transferred to a fresh reaction vessel, and both primers are then added to the fresh free strand to amplify by PCR.
In the present invention, the nucleic acid population is not isolated (such as by capture onto a solid phase) prior to contacting it with the oligonucleotide sequence(s). Thus each nucleic acid in the population may initially move freely in the suspension or solution in which it is contained. After contacting the nucleic acid population with the oligonucleotide sequence(s), preferably only the nucleic acid(s) which have correctly hybridised to the oligonucleotide sequence(s) are isolated (preferably by capture onto a solid phase).
In more detail, the method of this invention may comprise the following steps:
1. Restricting a large nucleic acid or population of large nucleic acids to generate fragments with known termini.
2. Ligating adaptors or linkers to the termini of these nucleic acid molecules. The ligated adaptor provides a known sequence at the termnini of a population of nucleic acids which can be used to design primers which extend beyond the terminal adaptor sequence into unknown sequence adjacent to the known adaptor sequence allowing the unknown sequence to be probed.
3. Optionally amplifying the adaptored fragments using primers complementary to the whole or part of the adaptor sequences at the termini of the adaptored fragments.
4. Optionally normalising the population of adaptored nucleic acids.
5. Selectively amplifying subsets of the nucleic acids through the use of pairs of primers which partially overlap into the unknown sequence. The overlapping primer will hybridise to a subset of the whole population. The size of the subset is determined by the length of overlap of the primer into the adjacent sequence.
The methods of this invention may be applied cyclically to sub-populations of sorted nucleic acids generated by the methods of this invention. Each cycle further reduces the complexity of the population. If necessary the cycles can be repeated until unique nucleic acid is obtained.
In a preferred embodiment the step of restricting nucleic acid is coupled to the ligation of adapters. Preferred restriction endonucleases for use with this invention cleave within their recognition sequence generating sticky-ends that do not encompass the whole recognition sequence. This allows adapters to be designed that bear sticky ends complementary to those generated by the preferred restriction endonuclease but which do not regenerate the recognition site of the preferred restriction endonuclease. This means that if the restriction reaction is performed in the presence of ligase and adapters, the ligation of restriction fragments to each other is reduced by continuous cleavage of these ligations whereas ligation of adapters is irreversible so the presence of adapters drives the restriction to completion and similarly the restriction endonuclease drives the ligation reaction to completion. This process ensures that a very high proportion of restriction fragments are ligated to adaptors. This is advantageous as ligation of adapters to restriction fragments is a relatively inefficient process. This is due to random ligation of restriction products to each other if these are phosphorylated. In this embodiment the adapters used are preferably not phosphorylated at their 5′ hydroxyl groups so that they cannot ligate to themselves. GB 9115407.0 describes a method of normalising a population of nucleic acids comprising the following steps:
1. Combining a mixture of heterogeneous DNA fragments with oligonucleotide primers compatible with some nucleic acid amplification system and denaturing the double stranded heterogeneous DNA.
2. Altering the conditions, i.e. reducing the temperature, to allow the more common species to re-anneal while preventing the primers from annealing to the DNA. The temperature for re-annealing at this stage must be higher than the melting temperature of the PCR primers.
3. Altering the reaction conditions further to allow the PCR primers to anneal to the remaining single stranded DNA which should represent the rarer species.
4. Performing strand extension of the primed species.
Advantageously, the above steps are applied cyclically a number of times to amplify the rarer species to a significant extent.
Application of this method to sequences with known termini permits the design of primers with very specific melting temperatures allowing the method to be used generically. Use of this method is particularly advantageous in reducing the complexity of genomic DNA as a significant proportion of most genomic DNA is repetitive sequence.
The advantage of providing a known sequence adjacent to probe sequence allows one to design libraries of probes, where all the probes in a library have the same melting temperature. This is advantageous as hybridisation of the entire library can be performed simultaneously at a single temperature whilst retaining the stringency of hybridisation.
Consider a large DNA fragment such as a mitochondrial genome or a cosmid or a microbial genome. To perform steps 1 to 4 of the method described above, such a large molecule can be cleaved with a frequently cutting restriction enzyme to generate fragments of the order of a few hundred bases in length. If a restriction endonuclease like Sau3A1 is used fragments with a known sticky end are left, to which double stranded adaptors can be ligated. These adaptors will bear a known primer sequence, and a sticky end complementary to that produced by the restriction endonuclease to permit ligation. A combined restriction and ligation protocol as described above is appropriate.
The majority of properly restricted fragments as a result bear an adaptor at each of their termini. This permits amplification of the adaptored restriction fragments at this stage if that is desired. After adaptoring and any non-selective amplification and normalisation, the nucleic acids can be differentially amplified to generate specific subsets of the starting population. The method of differential amplification preferably comprises the following steps:
1. Dividing the adaptored population of restriction fragments into separate wells. If, for example, primers with an overlap of a single base are used then the adaptored fragments would be divided into 10 or 16 wells.
2. Adding to each well one type of biotinylated primer of a predetermined set. The primer bears a sequence complementary to that provided by the adaptor and restriction site. The primer additionally bears an overlap of a predetermined number of bases beyond the known sequence into the unknown sequence immediately adjacent to the restriction site. Primers with different overlaps are added to different well. Four primers are need if a 1 base overlap is used. If 16 wells are used each of the 4 primers are added to 4 wells.
3. Denaturing the amplified fragment population that was subdivided into each well by raising the temperature. The temperature is then reduced to permit the primer sequences to anneal. Primers preferably have equalised melting temperatures so that conditions for use of all primers are the same.
4. Adding thermostable polymerase and nucleotides to extend annealed primers.
5. Capturing the biotinylated strand extension products from (4) onto a solid phase substrate derivitised with avidin. This may be effected through the addition of avidinated beads. These may optionally be magnetic beads.
6. Melting off the non-biotinylated complementary strand and washing this away. This leaves a single stranded copy of the selected fragments immobilised on the solid phase support.
7. To each of the separate pools is added one of the same set of primers as used in step (2) but not biotinylated, such that each pool receives a different combination of primers from this step and step (2). The primers should anneal to the single stranded capture molecules from (6). If 16 pools are used, to each is added one of the same 4 primers, but not biotinylated such that each of the 16 pools carries one of the possible different combinations of pairs of the 4 primers.
8. Extending the primed captured strands with polymerase and nucleotide triphosphates.
9. Denaturing the free strand from the captured strand by raising the temperature. The 'selected' free strand is thus released into solution. The liquid phase can be transferred to fresh reaction vessel or the solid phase support bearing the captured strands from (5) can be removed. This is very easy if the support used are magnetic beads as these can be removed by electromagnetic attraction to a probe.
The isolated free strands from (9) are thus isolated. At this stage the selected strands can be captured onto a solid phase support or amplified or the process of differential amplification can be repeated on the isolated subsets generated to further sub-sort these populations. This would be effected by using primers which overlap further into the unknown sequence adjacent to the known sequence of the adapter and the selected fragment. The sorted fragment could equally be cloned into a biological vector at this stage if desired.
Generating a captured library is advantageous in that it facilitates easy manipulation of the library of fragments. Such manipulations include copying, amplification and probing of the library for particular sequences. A captured library dispenses with any requirement for biological cloning vectors to maintain the library as such a library can be readily copied using polymerases and nucleotide triphosphates. The captured library can be readily washed and can very easily be stored in a refrigerated environment.
It should be noted in the example of primers that overlap by a single base, that the amplification products from the well containing a primer terminated by A followed by the primer terminated by G gives the complement of the well where G is followed by A. It might therefore be desirable to pool the reactions of where the same pair of primers are present but used in a different order to ensure that both strands of each DNA molecule are present and captured on the solid phase support. This would thus give 10 different pools. This is a convenient number as one can reduce the complexity of a library by one order of magnitude with four primers. Each sorted library of fragments can be further sub-sorted to an arbitrary degree.
An alternative embodiment of this method uses primers already immobilised on a solid phase support, preferably covalently linked to the support instead of biotinylated primers in step (2) of the differential amplification process. Such solid phase supports can be magnetic beads, as described in EP-A-0 091 453 and EP-A-0 106 873, or the support could be polymer beads. PCT/GB92/02394 describes a solid phase polymer support in a micro-column where the solid phase support are beads of silica gel. The beads are retained between two frits in the column through which solvents and reagents can flow. Such apparatus is also applicable with this invention.
One can clearly repeat the sorting process starting from a captured library that has been previously sorted.
One can also clearly use just 10 wells to generate sorted populations as all of the sequence information in a series of 16 wells will be present if just the 10 different pairs of primer combinations are used.
It should also be clear that labels can be introduced into sorted molecules by the primers used as part of the sorting process. Methods of introducing labels into primer oligonucleotides are well known in the art. Biotin has been discussed above, but many others are applicable.
One can also use probes which overlap beyond the provided adaptor sequence to any extent. It becomes more difficult, however, to ensure the stringency of hybridisation as the number of bases extending into the unknown sequence from the adaptor is increased.
To effect higher degrees of sorting one can either sort a sorted library with a set of four primers that overlap beyond the known terminal sequences by a single base or one can use primers with a longer sequence overlap. To sort an adaptored population of nucleic acid fragments using primers with a 2 base overlap beyond the adaptor sequence, the adaptored population of restriction fragments is sub-divided into 256 wells. In each well is one of 16 biotinylated primers which bear a sequence complementary to that provided by the adaptor and restriction site. The primers additionally bear an overlap of 2 bases beyond the known sequence into the unknown sequence immediately adjacent to the restriction site. The amplified fragment population subdivided into each well is denatured by raising the temperature and cooled allowing the primer sequences to anneal. Primers, again, preferably have equalised melting temperatures so that conditions for use of all primers is the same. Thermostable polymerase and nucleotides are added to extend annealed primers. Biotinylated fragments are captured onto a solid phase substrate via avidin and the complementary strand is melted off and washed away. To each of the 256 pools is added one of the same 16 primers, but not biotinylated such that each of the 256 pools carries one of the possible different combinations of pairs of the 16 primers. Again, AT followed by GC gives the complement of the reaction of GC followed by AT so it might be desirable to pool these pairs to give a total of 136 pools. For an overlap of n bases, one can distinguish 4n distinct sequences. If both termini of a molecule are used to select fragments then one can distinguish fragments into (4n×(4n+1)/2) distinct sets, since the orientation of each fragment is unknown.
Sorting a library resolves fragments from a large, complex population into defined sets whose size will be statistically regular and determinable as long as the size of the parent library is known, even if only approximately. The composition of the sorted library will be less complex than that of the parent library. This allows for useful manipulations of a large library without loss of information as all the sequences present in the starting library should be present in one of the sub libraries as long as long as all of the possible sub-libraries are generated. This method offers greater ease of manipulation of complex nucleic acid libraries and greater precision of manipulation than cloning into biological vectors.
To put this invention into practise requires the construction of probe oligonucleotides (ONs). Precise control over hybridisation conditions will be required to ensure clean results in differential amplification.
Details and reviews on the construction of labelled and modified ONs are available in numerous up-to-date texts, see references 1 to 6 below. A brief discussion of preferred design possibilities is given below.
There are major differences between the stability of short oligonucleotide duplexes containing all Watson-Crick base pairs. For example, duplexes comprising only adenine and thymine are unstable relative to duplexes of guanine and cytosine only. These differences in stability can present problems when trying to hybridise mixtures of short oligonucleotides to a target RNA. Low temperatures are needed to hybridise A-T rich sequences but at these temperatures G-C rich sequences will hybridise to sequences that are not fully complementary. This means that some mismatches may occur, and specificity can be lost for the G-C rich sequences. At higher temperatures G-C rich sequences will hybridise specifically but A-T rich sequences will not hybridise.
It is desirable that probes within a library behave in a similar manner, i.e. they should have similar melting temperatures and preferably also binding kinetics. In order to normalise these effects, modifications can be made to nucleic acids. Modifications fall into three broad categories: base modifications, backbone modifications and sugar modifications.
Numerous modifications can be made to the standard Watson-Crick bases. The following are examples of modifications that should normalise base pairing energies to some extent but they are not limiting:
The adenine analogue 2,6-diaminopurine forms three hydrogen bonds to thymine rather than two and therefore forms more stable base pairs.
The thymine analogue 5-propynyl dU forms more stable base pairs with adenine.
The guanine analogue hypoxanthine forms two hydrogen bonds with cytosine rather than three and therefore forms less stable base pairs.
These and other possible modifications should make it possible to compress the temperature range at which short oligonucleotides can hybridise specifically to their complementary sequences.
Nucleotides may be readily modified in the phosphate moiety. Under certain conditions, such as low salt concentration, analogues such as methylphosphonates, triesters and phosphoramidates have been shown to increase duplex stability. Such modifications may also have increased nuclease resistance. Further phosphate modifications include phophodithirates and boranophosphates, each of which increase the stability of ONs.
Isosteric replacement of phosphorus by sulphur gives nuclease resistant ONs (reference 7). Replacement by carbon at either phosphorus or linking oxygen is also a further possibility.
Various modifications to the 2′ position in the sugar moiety may be made (references 12 and 13). The sugar may be replaced by a different sugar such as hexose or the entire sugar phosphate backbone can be entirely replaced by a novel structure such as in peptide nucleic acids (PNA). For a discussion see reference 8. PNA may be the ideal choice as it forms duplexes of the highest thermal stability of any analogues so far discovered.
One major source of error in hybridisation reactions is the stringency of hybridisation of the primers to the target sequence and to the unknown bases beyond. If the primers designed for a target bear single artificially introduced mismatches the discrimination of the system is much higher (Zhen Guo et al., Nature Biotechnology 15, 331-335, April 1997). Additional mismatches are not tolerated to the same extent that a single mismatch would be when a fully complementary primer is used. Thus this can be exploited in the method disclosed above. If the probe used to extends beyond the provided sequence by 1 base, an artificial mismatch, 1 helical turn away from the probe base destabilises the double helix to a considerable degree if there is a second mismatch at the probe site.
Details on effects of hybridisation conditions for nucleic acid probes can be found in references 9 to 11.
Mass labels for use in the present invention are disclosed in patent application PCT/GB98/00127. Further labels for use in the present invention are discussed in the UK applications of Page White & Farrer file numbers 87820, 87821, 87900.
(1) Gait, M. J. editor, 'Oligonucleotide Synthesis: A Practical Approach', IRL Press. Oxford, 1990
(2) Eckstein. editor, 'Oligonucleotides and Analogues: A Practical Approach', IRL Press, Oxford, 1991
(3) Kricka, editor. 'Nonisotropic DNA Probe Techniques', Academic Press, San Diego, 1992
(4) Haugland, 'Handbook of Fluorescent Probes and Research Chemicals', Molecular Probes. Inc., Eugene, 1992
(5) Keller and Manack, 'DNA Probes. 2nd Edition', Stockton Press, New York, 1993
(6) Kessler, editor, 'Nonradioactive Labelling and Detection of Biomolecules', Springer-Verlag, Berlin, 1992.
(7) J. F. Milligan, M. D. Matteucci, J. C. Martin, J. Med. Chem. 36(14), 1923-1937,1993.
(8) P. E. Nielsen, Annu. Rev. Biophys. Biomol. Struct. 24, 167-183, 1995.
(9) Wetmur, Critical Reviews in Biochemistry and Molecular Biology, 26, 227-259, 1991
(10) Sambrook et al, 'Molecular Cloning: A Laboratory Manual, 2nd Edition', Cold Spring Harbour Laboratory, New York, 1989
(11) Hames, B. D., Higgins, S. J., 'Nucleic Acid Hybridisation: A Practical Approach', IRL Press, Oxford, 1988
(12) C. J. Guinosso, G. D. Hoke, S. M. Freier, J. F. Martin, D. J. Ecker, C. K. Mirabelle, S. T. Crooke, P. D. Cook, Nucleosides Nucleotides 10, 259-262, 1991.
(13) M. Carmo-Fonseca, R. Pepperkok, B. S. Sproat, W. Ansorge, M. S. Swanson, A. I. Lamond, EMBO J. 7, 1863-1873, 1991.
|Cited Patent
|Filing date
|Publication date
|13 Jul 1990
|21 Apr 1992
|Life Technologies, Inc.
|Amplification of nucleic acid sequences using oligonucleotides of random sequences as primers
|28 Oct 1994
|16 Apr 1996
|Queen's University At Kingston
|Indexing linkers
|14 Feb 1996
|16 Sep 1997
|Becton Dickinson And Company
|Solid supports for nucleic acid hybridization assays
|24 Oct 1995
|16 Feb 1999
|Curagen Corporation
|Method and apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing
|13 Jul 1998
|2 Oct 2001
|Brax Group Limited
|Categorising nucleic acids
|16 Nov 1989
|30 May 1990
|Eastman Kodak Company
|Diagnostic kit and method using a solid phase capture means for detecting nucleic acids
|26 Mar 1996
|2 Oct 1996
|Research Development Corporation Of Japan
|Method for molecular indexing categorising of expressed genes using restriction enzymes
|13 Jul 1993
|20 Jan 1994
|Medical Research Council
|Process for categorising nucleotide sequence populations
|Dynal, Technical Handbook Second Edition, 1995, p. 116-119.*
|Guilfoyle et al., Nucleic Acids Research, vol. 25, No. 9, May 1, 1997, pp. 1854-1858, XP 002076198.
|Wong et al., Nucleic Acids Research, vol. 19, No. 9, May 11, 1991, pp 2251-2259, XP000204316.
|U.S. Classification
|435/6.12, 435/91.1, 536/23.1, 536/24.33, 435/91.2
|International Classification
|Cooperative Classification
|European Classification
|10 Apr 2000
Owner name: BRAX GROUP LIMITED, UNITED KINGDOM
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:SCHMIDT, GUNTER;THOMPSON, ANDREW HUGIN;REEL/FRAME:010828/0408
Effective date: 20000215
|14 Nov 2001
Owner name: XZILLION GMBH & CO., GERMANY
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:AVENTIS RESEARCH & TECHNOLOGIES GMBH & CO. KG;REEL/FRAME:012306/0120
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Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:BRAX GROUP LIMITED;REEL/FRAME:012306/0341
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|11 Apr 2002
Owner name: XZILLION GMBH & CO., GERMANY
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:AVENTIS RESEARCH & TECHNOLOGIES GMBH & CO. KG;REEL/FRAME:012784/0356
Effective date: 20010510
|11 Jul 2007
|Maintenance fee reminder mailed
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|28 Dec 2007
|Fee payment
Year of fee payment: 4
|8 Aug 2011
|Maintenance fee reminder mailed
|30 Dec 2011
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|21 Feb 2012
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Effective date: 20111230


text:
Patrick Stewart and Ian McKellen recently did a press interview for X-Men together and the result is, as usual, a complete and utter delight. While the interview is fairly serious (the two discuss why the X-Men appeals to minorities as well as the current response to homosexuality in the UK), Professor Xavier and Magneto also spend some time coming up with hashtags for their bromance, giggling at bathroom jokes, and discussing their feelings about old age.
Image via Getty


text: London fashion week Inside Paul Smith's 'department of silly' Sorry, your browser is unable to play this video. Please install Adobe Flash™ and try again. Alternatively upgrade to a modern browser. Fashion designer Paul Smith, the master of classic but quirky clothes, allows Simon Chilvers into the off-limits areas of his London design HQ Rebecca Lovell and Simon Chilvers, Source: Staff and agencies Wednesday 24 February 2010 05.20 EST Share on Facebook Share on Twitter Share via Email Share on Pinterest Share on LinkedIn Share on Google+ Share on WhatsApp Topics London fashion week Fashion weeks Reuse this content


text:
Having spent the past decade forecasting the economy, I am accustomed to being wrong about my predictions. So, I ask readers to forgive my gloating a bit when I correctly foretell the future.
I am talking, of course, about the recent school referenda in Indiana. Let me explain.
Since the passage of property tax caps in 2008, I have prophesied a day in which local governments became more adept at their fiscal management and local taxpayers more supportive of higher taxes. The May 2014 elections suggest that day is coming.
In this election cycle, only one of 10 school corporations saw its referendum fail. Nine other districts won additional property taxes for a variety of construction and operations efforts. This loss was understandable.
Of the 10 school corporations asking for referenda, seven had raw performance or student growth scores that were above average in the most recent year. All of these won their referenda. Of the three school districts with below-average achievement and student growth, two school corporations asked for operating funds, primarily for transportation. They both won.
Madison Consolidated Schools asked for a large tranche to build a new gymnasium and choral rooms along with other infrastructure improvements.
Unfortunately, Madison had arguably the worst school performance of the 10 seeking referenda in this election, and there's not much to suggest they are improving. The measure lost badly, with three out of four voters opposing the measure.
We should draw two lessons from these recent referenda.
First, the vigorous reform efforts of 2007 through the present, including the tea party movement and numerous small constituencies across the state, were never really an anti-tax movement. They were something altogether bigger and more important. What we have seen in Indiana and elsewhere is broad frustration over the value of government, not simply its cost.
Second, those school corporations that won their referenda were on average better performing than those who lost. I also think they did a better job of explaining their needs to taxpayers, but this is only a hypothesis because we cannot directly measure that.
These two lessons have potentially enormous implications for the future of Indiana local government. We must think them through carefully, but I think a few issues are clear.
The much better state and local tax burdens we have since 2007 are not the end to grassroots efforts to improve the value of state and local government.
Anti-tax zeal will (hopefully) be replaced with the far more difficult effort of holding state and local leaders accountable for value in public goods and services.
This is critical because the gravest challenges to prosperity in many Indiana communities are not high taxes but low-quality public services. Any effort to foster growth through public policy will have to come primarily from better public services, not from tax cuts.
I have long argued Hoosier taxpayers are willing to spend more in places where they can see results. The results of the recent election suggested I am right about that.
Michael J. Hicks is the director of the Center for Business and Economic Research and an associate professor of economics in the Miller College of Business at Ball State University. Send comments to email@example.com.


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...as I've been around but it's been awhile, hehehe Just wanted to come here first since it's been sooo long since I was around just to say hello to everyone again & that I've missed ya but geesh, life has been busy. Plus, I guess what little free time I've had has been spent facebooking but that's getting old & I miss talking about WDW!
I sure hope everyone is doing well & can't wait to catch back up with the 'old gang' (not saying anyone here is OLD by any means :tongue.
I'm sure I'll be posting away now so I'll be seeing you in the threads or drop me a PM & lets catch up!!


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Ok….so this post is dedicated to our friends that showed us this trick….and we are so grateful for their great idea. It worked for us (I'm still surprised myself), and I hope it works for you too! Thanks Wood Family 🙂
Ok so last year at this time we embarked with a new year's resolution that honestly I didn't think we would be able to keep up….how's that for a resolution! This resolution was to read scriptures every day as a family….not a whole chapter, not even a whole ten verses, just at least one verse. We just wanted to make family scripture study a habit, whether it was one verse or 20. We had tried in the past to make this a habit with no real success or consistency. BUT WE DID IT THIS YEAR! We missed only 2 days! That's not to say that there wasn't fighting and wrestling and rough housing and crying on some or all of those days; but that's the beauty of it, that's just how it is, for us at least…heavens…my kids are 7, 6, 5, and 3. 🙂 Let's be honest, it's like herding cats. If we can do this, ANYONE can do this, trust me.
SECRETS TO OUR SUCCESS (isn't that an 80's movie?): There are definitely secrets, but they are "EASY PEASY LEMON SQUEEZY", as my kids would say.
- BE ACCOUNTABLE: This is the BEST SECRET of them all, I tell ya…use one page CALENDAR CHART that represents the whole year!!!!!! Dave and I said so many times throughout the year that just the thought that we were going to have a gap on our reading chart was enough to muster up the umph to read each night, and how we didn't anticipate that we would be motivated by that, but it worked.
- USE COLORED STICKERS & make it a game: This was also a main key for us. The child that was the most well-behaved got to choose the sticker for the chart that night. This was a major motivator for our kids.
- CHOOSE THE SAME TIME EACH DAY TO READ: For us, the time of day we chose wasn't exactly at a specific specific time, but right before bed which was around the same time each night. If you can choose the same time each day that works best for your family, you are really setting yourselves up for success.
- KEEP YOUR EXPECTATIONS LOW: Ok this is one of those times that low expectations are GREAT. The kids are going to be rowdy, mean, ornery, and not want to do it at times…hang in there :). There should be a level of respect while reading, from my point of view, but it's not going to be perfect. Sometimes the best things are HARD, until it's a habit. Just tonight as we were reading, the younger two got into trouble because they were being too loud, it happens still, but at least we are reading, right!
Boyd K. Packer, in a talk titled, Counsel to Youth (can be found there), said, "I say again that youth today are being raised in enemy territory with a declining standard of morality." This is so true. I believe that a loving Father in Heaven communicates to his children through prayer & scripture. I want my kids to have this open communication with their Savior. I know without a doubt that learning to be more like God will help them more in their lives than learning to be like any other person they could model themselves after. Since my job as a parent is to equip my children with the best tools possible for success, this is top priority.
(the thing that worked best for us is to print this out, front and back, on a piece of white card stock and laminate)
DOWNLOAD FRONT (8.5″x 11″): HERE
DOWNLOAD BACK: (8.5x 11″): HERE
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kellie Says: January 4, 2012 at 8:48 am
This is so great! I love how you just have the date, instead of certain verses you "should" be reading each day. I think I can manage this with my young children. Thank you!
Thank you for the cute printable. We are going to do our best this year to stick to it! We are way too off an on with reading scriptures at home. I love the saying on the back of the printable, sooo true!
What a great idea! Thanks for sharing.
tricia Says: January 4, 2012 at 9:34 am
I couldn't get the printable to print? Dp I have to sign into the box?
tricia Says: January 4, 2012 at 9:37 am
I figured it out…I had to sign out of my works vpn…
Thank you so much for posting this! It's something we've struggled with, too, but REALLY want make a habit of. Thanks for the printable and inspiration to keep at it! 🙂
jane Says: January 4, 2012 at 11:21 am
Scriptures, Prayers & Fhe ~ what is Fhe?
Family night 🙂 a night dedicated to just our family, one night a week where we have a gospel message and do things like games, service, make a treat, go out and do a fun activity, and just play together and spend time together :).
Deb Says: January 4, 2012 at 12:20 pm
love it! thank you 🙂 I love the temple box, too. brilliant!
Kim Says: January 4, 2012 at 1:47 pm
Boy, Mr. Packer is so right on. Thanks so much for the link to Counsel to Youth. I, myself have 7 children and out of them, the three oldest are teenage girls. It is so hard in this day and age for our youth, our teens. My dad told me the other day, "boy I am sure glad that I am not raising kids in this day and age." It is hard, and a constant thing to keep up with and maintain, but I do it. I love my children dearly and will keep on. The Good Lord gave them to me and it's my job to do my best. We have a scripture reading night on Sundays and the kids have enjoyed it. I get the rowdy and rambunctious like you do with my little ones and then the teens say sometimes, "ah, come on, we are tired, lets just say a quick prayer and off to bed." But, I maintain and keep on with it. Then, one night, I overheard one of the girls in their room, say, "ya know, I really enjoy our family time on Sundays and that mom and dad love us so much that they do this and have us read the scriptures, it gives me my weapon to go out and face the week." The other 2 agreed with her. So, they may moan, groan, get rowdy and loud, but the message is getting to them. I was always told by my parents to keep on talking, keep on preaching, that they hear you, even though you think they don't.
Thanks so much for the share and I am gonna bring it up and see if my bunch want to make it a daily thing or at least maybe 3 times a week thing, instead of just on Sunday night. But, whatever happens, I will keep on fighting the enemies out there and give my kids good morals, respect, kindness, and love, and have them pass that along in life.
So awesome! Great idea. Pinned it ; )
Dezi A Says: January 4, 2012 at 11:09 pm
I love this!! Thanks so much!
Brittany Says: January 6, 2012 at 10:12 am
What a great chart! Thanks for sharing. We had a similar struggle trying to figure out how to have daily family scripture study. We decided to start with reading just one or two verses a day before bedtime. It has been great! Our almost-3-year old won't let us end the night without reading from her scriptures.
This is a great idea! Thank you! We do the gather them on the couch version as well. But this worked excellent! What we did was print out a scripture for each month. We picked the primary 2011 scriptures last year, and the 13 articles of faith the year before, this year we did the 2012 primary scriptures (to learn more go to lds.org) . I printed them in 2×2 inch squares. Then I put them together and laminated them. Punch a hole in the top and put it on a key ring. I actually made two- one for the house- hung on our FHE board, or key rack, and one for the car, which actually wound up attached to my purse. Now we are never without scriptures! If we missed it in the morning, or we are traveling, we have no excuse! We say the scripture of the month every day- so that we memorize it. Once they have it memorized, we try to go back and do others. It is a great opportuinity to talk with kids about a specific scripture for an entire month. Hope this makes sense! it has been such a blessing, and such a non stress way to study the scriptures, even my 3 year olds memorized all the scriptures and articles of faith! Letha
That's such a great idea! We so often underestimate what our children are capable of, don't you think?? I love this idea, maybe we will incorporate this somehow :). THANKS!
Em Says: January 6, 2012 at 1:46 pm
Hi! I LOVE this, and I would love to print this a little bigger (like poster size), is there a way to get it at a higher resolution? Thanks!
shay Says: January 6, 2012 at 4:31 pm
printed it off, thanks so much i was just thinking about somehting like this last night! its great! thanks for sharing your talents and DITTO on the POSTER SIZE!!!!!???//
I am adding the poster size option right now! ENJOY! I am uploading it as a 16×20 and 20×24, 🙂
Jamey Says: January 31, 2012 at 11:18 am
Sometimes the only time we are together is at dinner time. So we have started to listen to the scriptures while we are eating dinner. We have it on our iPod and we will listen to about 10 minutes of scripture while we eat, and then we turn it off and talk about it. We told the kids that after so many days, we will have an ice cream sundae party while we listen to them for FHE night! It seems to work, the kids remind me if we forget.
Trina Says: May 31, 2012 at 12:25 pm
Thank you for sharing this! I just had my first year anniversary with my husband and with all the good that the first year brought we realized that we didn't read our scriptures as consistently as we would have liked to. We don't have any children yet, but I'm excited to use this just for us! I think this will help us get into a more consistent pattern of scripture study that will prepare us to be parents one day. Thanks again!
Sara Says: September 24, 2012 at 7:41 pm
Hello! I love this printable chart! I'm so sad that I just discovered it with only 3 months left in 2012–would you PLEASE consider making one for 2013?? (And I would absolutlely love an email if you do, so that I don't forget to check back! ) 🙂 Thank you!
Evelyn Says: June 23, 2013 at 4:19 pm
I just found this website and I LOVE the chart. Do you happen to have one for 2013?
Evelyn Says: June 23, 2013 at 4:24 pm
Never mind. I found the printable for 2013. THANKS!!!!!!
[…] can read Kirsten's experiences and advice for 2012, 2013, and 2014. If this is your first year using the chart, here are some tips to help it become […]


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Israeli Raid Turns into Major Clash
A raid by Israeli troops to arrest three men inside Palestinian territory has turned into a large-scale armed confrontation, as Israeli troops, tanks and helicopters become involved. The raid inside Palestinian territory comes as US peace envoy Zinni is starting his talks with Israeli Prime Minster Sharon and Palestinian President Arafat.
The raid begun in the early morning hours as Israel Special Forces moved into the village of Til, southwest of Nablus, to arrest three Palestinians suspected of planning to carry out a terror attack. the Itim news agency reported. In similar raids elsewhere on Thursday, Israeli forces had already seized five suspected Hamas militants.
Tel Mayor Adnan Sefi told the Reuters agency by telephone that between 15 and 20 Israeli armored vehicles including tanks rumbled into the village, near the city of Nablus, at dawn. However, the Til village raid was different. Israel Army Radio said that a firefight broke out, during which one of the Palestinians was killed and the other two wounded.
The Jerusalem Post reported several hours later that Israeli soldiers were still taking part in the manhunt after several Hamas and Islamic Jihad activists, and that helicopters and tanks were also being used. The Israeli Army has declared the entire vicinity a closed military zone, preventing journalists from approaching. (www.albawaba.com)


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Four out of every 10 Mexican adults surveyed would migrate to the United States if they had the means and opportunity to do so, according...
WASHINGTON — Four out of every 10 Mexican adults surveyed would migrate to the United States if they had the means and opportunity to do so, according to a poll released yesterday.
And two in 10 Mexican adults surveyed say they would be willing to live and work illegally in the United States, the Pew Hispanic Center reported in what is believed to be the first snapshot by U.S. pollsters of Mexicans' views on migration.
With about one in every eight Mexican adults already living in the United States — and 40 percent of the nation's nearly 70 million adults willing to migrate if the opportunity presented itself — the findings could hold implications for U.S. policy-makers.
Congress and the Bush administration are considering various proposals for a temporary-worker program that would allow foreign workers to come here and let many of the more than 11 million illegal immigrants remain here for a number of years before returning home. Of the 10 million Mexicans living in the United States, about half are believed to be here illegally.
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While President Bush has said he wants to begin the debate this fall, the prospects for enacting a guest-worker plan — which has proven controversial among lawmakers who fear it would reward lawbreakers and spur new illegal immigration — remain unclear.
Two-thirds of the 2,400 adults surveyed in Mexico for the Pew Hispanic Center said their friends and relatives would be interested in participating in a temporary-worker program. The survey had a margin of error of plus or minus 3 percentage points.
The survey dispels the belief that Mexicans with the least opportunity at home are most likely to head north in search of better lives. One-third of college-educated Mexicans and half of those with a high-school education also voiced a desire to migrate.
Though Mexico's educational system has improved, its economy has yet to generate enough jobs sought by people with more education, said Roberto Suro, executive director of the Pew Hispanic Center.
"People with a high-school or even a college degree in Mexico believe that they have greater economic opportunity migrating to the U.S. — even illegally — than they would staying at home," he said.
Mexican government officials were examining the study, a Mexican Embassy spokesman said, and had no immediate comment.


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NewsHour Poetry Series
One million Syrian children have been ripped from the daily life they know and forced them into refugee camps. Margaret Warner speaks with Sarah Crowe of UNICEF for more on the impact of the war on the youngest Syrians and the burden the crisis has placed on the surrounding region. Continue reading
United Nations Secretary-General Ban Ki-moon initiated a formal request to the Syrian government to be able to investigate an alleged chemical weapons attack on civilians. Margaret Warner reports on how countries like France have responded and debate in the United States over the appropriate response. Continue reading
Newly released audio recordings offer a look inside the Nixon presidency as the Watergate scandal was unfolding. In one excerpt, President Richard Nixon has just announced the resignation of his two top aides. Judy Woodruff talks to veteran journalist Marvin Kalb and Ken Hughes of the University of Virginia's Miller Center. Continue reading
If United Nations investigators prove that the Assad regime has used chemical weapons in a recent attack on Syrian civilians, do Western nations have a moral obligation to act? Margaret Warner gets debate from Robert Zarate of the Foreign Policy Initiative and Joshua Landis of the University of Oklahoma. Continue reading
Amateur videos show scores of Syrian civilians suffering after what opposition leaders say was a deadly, poisonous gas attack by government forces outside the capital city of Damascus. As in past accusations, Syrian government officials are denying any use of chemical weapons. Ray Suarez reports. Continue reading
Will investigators find sufficient evidence of chemical weapon use by the Syrian government in this latest alleged attack on civilians? What will inspectors face on the ground? Ray Suarez asks Jeffrey White of the Washington Institute for Near East Policy and Amy Smithson of the James Martin Center for Nonproliferation Studies. Continue reading
Editor's note | Starting Sept. 7, the PBS NewsHour is expanding its family, adding a "PBS NewsHour Weekend" newscast on Saturdays and Sundays. The 30-minute show will be anchored by NewsHour senior correspondent Hari Sreenivasan. The above interview is a … Continue reading
The second largest refugee camp in the world, home to 120,000 people, is in Jordan, flocked to by Syrians displaced by the civil war. Lindsey Hilsum of Independent Television News spends a day in the camp known as Zaatari, where residents cope with difficult conditions and feel little hope for returning home in the near future. Continue reading
A rebel attack on an army-controlled district in the key city of Homs showed that the Syrian opposition is still on the offense in the face of recent gains by government forces. Meanwhile, President Bashar al-Assad issued a statement expressing confidence his forces will win the civil war. Margaret Warner reports. Continue reading
In Syria, the Assad regime has made major gains in the central part of the country while rebel forces remain strong in the north and south. Margaret Warner talks to NPR's Deborah Amos about how both sides are appealing to their bases with imagery, the resiliency of Syrian civilians and if there an end in sight for this conflict. Continue reading


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One week later and still no winner has come forward to claim that $57 million Mega Millions jackpot. The winning ticket was purchased at the Kangaroo Express at 1531 SE Greenville Boulevard in Greenville.
North Carolina Education Lottery spokesman Van Denton says it's not unusual for someone to take this long to claim a big jackpot because it is a life-changing event. That person has 180 days from last Friday to claim the prize at lottery headquarters in Raleigh.
The winning numbers were 3 - 19 - 21 - 44 - 45 with the Mega Ball 29.
The ticket is worth an estimated $57 million as an annuity or $42.2 million cash--minus state and federal withholdings--if the winner elects to take the lump sum.
So far there have been four big jackpot winners in North Carolina. Three of those were Powerball jackpot winners. Jackie Alston of Halifax who won $74.5 million in November 2006; Jeff Wilson of Kings Mountain who won $88.1 million in June 2009; and Frank Griffin of Asheville who won $141.4 million in February 2010. The first Mega Millions jackpot winner was retired Greensboro businessman Mike Manheim who won $12 million when all of his numbers came up May 2010.
That big $74.5 million winner took 2-1/2 months to claim the jackpot back in 2007.


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Kayla Tausche is an on-air reporter based at CNBC Headquarters in Englewood Cliffs, NJ. Tausche is also a member of the ensemble cast of CNBC's "Squawk Alley" (M-F, 11AM-12PM ET) where she focuses on the big money backing technology.
Tausche has covered the banking industry, as well as corporate finance and deals— and frequently breaks news. Since joining CNBC in 2011, she has reported on a wide variety of high-profile stories, including the Facebook and Twitter IPOs, the Occupy Wall Street movement, the MF Global bankruptcy and the UK phone hacking scandal.
She reports across NBC properties as a contributor to MSNBC, "TODAY" and "NBC Nightly News." In addition to reporting, Tausche serves as a substitute anchor for flagship CNBC programs "Squawk Box," "Squawk on the Street" and "Power Lunch."
Previously, Tausche was based in London as the assistant editor of DealReporter, a Financial Times-owned publication, covering mergers and acquisitions. Prior to DealReporter, she worked on the consumer and retail beat at Bloomberg News. She began her career in journalism at the Brussels bureau of The Associated Press, where her bilingual interview experience included Jacques Chirac and Peter Mandelson.
An Atlanta native, Tausche graduated with honors in business journalism and international politics from University of North Carolina at Chapel Hill, where she was an Ameel J. Fisher scholar. She sits on the alumni boards of the UNC journalism school and the Steamboat Foundation, where she was a fellow.
Follow Kayla Tausche on Twitter @kaylatausche .
Wells Fargo has banned its employees from loaning their own money on peer-to-peer lending platforms. CNBC's Kayla Tausche explains the risks involved with the lending strategy.
The winners from the fourth quarter are underdogs, companies that have for years been cleaning up messes. Find out who they are.
CNBC's Kayla Tausche reports how Goldman Sachs has become one of the top attractors for young talent on Wall Street.
A provision prohibiting banks from keeping investments in CDOs backed by hybrid securities called trust preferreds ignited a controversy.
Bank of America is on the rise after reporting stronger-than-expected earnings. CNBC's Kayla Tausche reports BofA is saving $1.5 billion a quarter on legal expenses and revenue rose across each business segment last quarter.
CNBC's Kayla Tausche reports Bank of America's litigation expense rose to $2.3 billion.
CNBC's Kayla Tausche reports on mixed earnings results from the big banks. Wells Fargo managed to beat the street and JPM did not have incredibly positive earnings.
CNBC's Kayla Tausche has the latest details from JPMorgan's media call. The company had to sell a bunch of assets to bolster the bottom line in light of some litigation expenses, reveals Tausche.
A revelation by Target showed its data breach spanned far wider than expected, raising questions about exactly how such an expansive hack took place.
Happy Thursday. Time to start getting those nonfarm payroll guesses together. It's only a day away.


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RECIPE: Margarita Lime Soap Loaf
This soap looks just like a margarita - even down to the salt on top of the glass. The fragrance of margarita lime is very light and refreshing.
Project Level:
Estimated Time:
1 Hour
40-44 oz. loaf
• 62.5 Ounce(s)
Premium Extra Clear MP Soap Base - 2 lb Tray
• 2 Ounce(s)
Glycerin - Natural
• 1/4 Teaspoon(s)
Neon Yellow Hello Powder
• 1/4 Teaspoon(s)
Matte Woodland Green Pigment Powder
• 2 Ounce(s)
Margarita Lime Fragrance Oil 472
• 2 Tablespoon(s)
Salt Blend - European Sea (Pure Salt)
• 1 Tablespoon(s)
Iridescent Super Sparkle Glitter
• 1/4 Teaspoon(s) White Sparkle Mica Powder
• 1 Piece(s)
Round Guest Silicone Mold 1607
• 1 Piece(s)
Regular Loaf Silicone Mold 1501
• Large and Small Glass Measuring Cups
• Measuring Spoons
• Microwave or Double Boiler
• Mixing Spoons
• Spray Bottle with alcohol(for spritzing)
Recipe Directions
NOTE: If you are making these products for sale, good manufacturing practices recommend you wear a hairnet, gloves and a mask. We also recommend a clean apron.
For Limes: In 2 small glass bowls or plastic beakers, mix 1 oz. of glycerin with 1/4 teaspoon of Matte Woodland Green pigment powder. Mix 1 oz. glycerin and 1/4 teaspoon Neon Laser Lemon dye powder.
Before turning on the mini-mixer, gently blend powder into glycerin by hand. Once powder is wet, turn on mini-mixer and blend until all powder dye is dissolved. This will prevent powder from becoming airborne.
Weigh 2 oz. of Extra Clear soap base and place in glass measuring cup. Heat soap in microwave until it has melted. Add 1/4 teaspoon Whie Sparkle White and stir.
To white soap, add a small amount of matte woodland green. Add enough lemon color to get a nice lime shade. Pour soap into bottom of loaf mold. Spritz with alcohol to dissolve surface bubbles. Allow to set up.
Once soap has set, carefully remove from mold. Cut into strips that are 5" long and 3/4" wide. These will be the rind and peel for the lime slices. You will only need 4 lime slices which are cut into 6 thinner slices per lime.
Melt about 2 oz. of Extra Clear soap base in microwave.
Taking the Round-Guest-Silicone mold, working one cavity at at time, pour just enough clear soap to cover the bottom.
Working quickly, take one lime rind strip and spritz an edge of the strip with alcohol. Place the strip (alcohol-side down) into the melted clear soap, lining the circumference of the cavity with it. Spritz again with alcohol.
Repeat this step with 3 additional soap cavities.
Melt 4 oz. of clear soap. Add same mixture of woodland green/lemon yellow color to make lime shade. Blend in well.
Add 2.5 ml of Margarita Lime fragrance oil and mix. Spritz inside of each cavity and pour melted lime green soap into each. Spritz tops of each to release air bubbles. Allow to set.
Once limes have set up, remove from mold. Cut each slice in half diagonally. Taking one half, stand on end (flat side down) and slice into thirds. Repeat with other half.
Continue until all limes have been cut into 6 slices. There should be a total of 24 lime slices.
For Ice Cubes: Cut up 16 oz. of extra clear MP soap and place in glass measuring cup. Heat in microwave until melted. Add just a pinch of White Sparkle Mica to give ice cubes an opaque look.
Add .5 oz. of Margarita Lime fragrance oil and mix well. Pour into Silicone Loaf mold and spritz top with alcohol. Let soap set up.
Once soap has completely hardened, carefully remove from mold. Cut soap up into small pieces to look like crushed ice. Reserve about 1/2 cup for top of soap.
Weigh and cup up 25 ounces of clear soap. Heat in microwave until melted.
To melted soap, first add Neon Laser Lemon mixture. Then add small amount of Matte Woodland Green mixture to create a lime color. Add .75 oz. of Margarita Lime fragrance and mix well.
Into bottom of loaf mold, pour enough lime soap to cover bottom and spritz with alcohol.
Generously spritz ice cubes with alcohol and place into mold. Pour lime green soap over chopped ice. Do not pour all the way to the top. You will need to leave room for the lime slices and salt on top of loaf.
When soap sets up a bit and starts to thicken, place lime slices into soap. They should suspend in the top of loaf. Be sure part of the slice is sticking out of the top.
While soap is setting up, mix 2 tablespoons of European Salt Blend with 1 tablespoon of iridescent glitter. Spritz salt with alcohol generously while mixing with glitter.
Once loaf has hardened, melt 1 oz. of clear soap in microwave. Pour a thin layer over the top of loaf.
Sprinkle crushed ice over the top and follow with the salt/mica mxture. If topping is too loose, sprinkle a little more melted clear soap on top.
Once completely hardened, remove from mold. Using mitre box, if available, slice soap with soap cutter. Loaf should yield 8-1 inch slices.
Wrap soap tightly with plastic wrap or shrink bags. Label accordingly.
INCI Ingredient Label:
Propylene Glycol, Sorbitol, Water, Sodium Stearate, Sodium Laureth Sulfate, Sodium Myristate, Sodium Cocoyl Isethionate, Triethanolamine, Glycerin, Titanium Dioxide, Fragrance, Polyester-3, Yellow 7, Chromium Oxide, Sea Salt, Polyethylene Terephthalate Copolymer
Wholesale Supplies Plus is not responsible for the products you create from our supplies. You alone are responsible for product and recipe testing to ensure compatibility and safety.
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In these straightened times a main concern for many companies is how to raise finance for acquisitions, refurbishments or new builds.
We advise on debt and equity, steering you through the funding options to make sure you choose the right source for your needs. We can also help with refinancing and restructuring loans, claiming capital allowances to strengthen your business cash flow position, supporting your needs in challenging market conditions.
We also specialise advising on building consent applications, claims for business property relief (BPR) and compliance issues. Our team have built a niche expertise analysing and advising on complex rules concerning VAT planning in relation to the acquisition, sale and development of land and buildings. We're now recognised by property developers, construction professionals and HM Revenue & Customs as market leaders in this specialist field.
We can help with:
- Accounting
- Internal and external audit
- Fundraising
- Investment
- VAT planning for property development or renovations
- Advising on tax allowances for commercial property
- Reviewing business structures
- Establishing joint ventures
- Maximising Business Property Relief and Capital Allowances
- Feasibility studies
- Tax planning
- Compliance
- Corporate finance
- Exit strategies
- Business insolvency
International capabilities
To serve clients operating overseas we have an International Tax team with over twenty years' experience. We're also independent members of PKF Limited giving us access to a global network of organisations supporting your expansion plans.
In order to maintain continuity of the specialists you will work with, Johnston Carmichael has appointed a Head of Construction & Property, Michael Murray. Michael has over 15 years' experience in the sector acting for owner-managed businesses, single asset investors, property companies and life funds and leads the Construction & Property team for the firm.
- Alistair BlackConsulting Director
- Brian MainGeneral Practice Partner
- Colin McKelvieTax Partner
- Craig MacPhersonGeneral Practice Partner
- Graham ReidAudit Director
- Irvine SpowartAudit Director
- James CampbellGeneral Practice Partner
- Jamie WaughAudit Partner
- Jim PorterGeneral Practice Partner
- Lynne WalkerAudit Director
- Michael MurrayTax Director - Capital Allowances
- Mike PitmanGeneral Practice Partner
- Robert WintersTax Senior Manager - Capital Allowances
- Roddy AndersonGeneral Practice Partner
- Scott HolmesAudit Partner
- Shaun MillicanGeneral Practice Partner
- Stephen DickieGeneral Practice Partner
Changes in Property Taxation
27th January 2016
In the space of six months there has been a sea of changes annouced in property taxation including a particular attack on the private landlord. The changes will shortly be ...read more
Land and Buildings Transaction Tax (LBTT) – what do you need to know?
21st April 2015
Land and Building Transaction Tax (LBTT) is replacing SDLT for land transactions in Scotland. The framework of LBTT follows that of SDLT and there are many similarities.read more
Sharp Minds on Construction and Property
9th April 2015
In these straightened times a main concern for many companies is how to raise finance for acquisitions, refurbishments or new builds. Download our Construction & Property brochure to find out how we can help you.read more
Stamp Duty Land Tax is being stamped out!
23rd January 2015
Remember that from the 1st of April land and buildings transaction tax (LBTT) will replace stamp duty land tax (SDLT) for all Scottish land transactions. This will result in significant changes to the amount of tax payable.read more
Johnston Carmichael Corporate Brochure
9th September 2014
Our business and your business combined opens a world of opportunity – it's that sense of partnership that has defined us since 1936. For a full overview of our services and our sector expertise please download a copy of our Corporate brochure below.read more


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This content has been marked as final. Show 1 reply
1. Re: DG4MSQL to connect 2 seperate database instances of MSSQLKgronau-Oracle Jul 9, 2013 6:38 AM (in response to 1022342)
You have to duplicate the whole configuration which means you have to create a new listener SID entry with its own, dedicated SID. This SID now needs its own gateway init file where you specify the new SQL Server instance. Finally on the database side you have to add a new tnsnames.ora alias where you make sure the SID refers to the newly created gateway SID.
when you add a new SID to the listener.ora file, please make sure to STOP and START the gateway listener.
- Klaus


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SAN RAFAEL - Dominican University of California is receiving a $989,798 grant from the 2008 Farm Bill to establish the National Ornamentals Research Site at Dominican University.
Scientists from the national and international research community will conduct studies focused on understanding and controlling Phytophthora ramorum, the plant pathogen known to cause Sudden Oak Death and ramorum blight on nursery stock.
The research site will be the first in the United States dedicated to the study of diseases of ornamental plants in a simulated nursery setting.
The grant is administered through the United States Department of Agriculture, the Animal and Plant Health Inspection Service, Plant Protection and Quarantine, and the Center for Plant Health Science and Technology. The grant is the largest that the Center for Plant Health Science and Technology has ever awarded to a single organization in one year. While the grant provides one-time funding, Dominican can re-apply each year through the 2008 Farm Bill for additional funds.
"The positive economic impact that research conducted at this site will have on the nursery industry cannot be overstated," said Dr. Sibdas Ghosh, associate dean for academic development in Dominican's School of Health and Natural Sciences. "This site will provide valuable data that will aid in reducing long-range spread of Phytophthora ramorum through infested nursery stock shipments."


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Traulsen G20010 Description
The Traulsen G20010 two-section refrigerator is 53 inches wide and 32 inches deep, offering 48.8 cubic feet of cold storage space for busy commercial kitchens. Like every G-Series "Dealer's Choice" commercial refrigerator, it is constructed with a stainless steel front and doors and anodized aluminum sides and interior finishes. The solid full-length doors are self-closing and come standard with locks and a 120 degrees stay-open feature. Three factory-installed, epoxy-coated shelves are included and adjustable by the user. This commercial refrigerator is ideal for busy kitchens due to its thermostatic expansion valve that offers quick and responsive temperature control.
Product Details
- Stainless steel exterior front and doors for durability and ease of cleaning
- Anodized aluminum interior and sides for durability and corrosion resistance
- Top-mounted refrigeration system is self-contained
- Solid full-length doors with locks for optimal insulation and safety
- Self-closing doors to ensure it shuts after use
- Door stays open at 120 degrees for easier inventory checks or stocking
- Cabinet and doors insulated with non-CFC, foamed in place polyurethane for better insulation
- Heated door perimeters to avoid condensation
- Left door is left-hinged and right door is right-hinged for better access
- Thermostatic expansion valve to for a faster refrigerator recovery time
- Incandescent interior lights that automatically activate for user's convenience
- Cam-lift hinges and horizontal door handles, guaranteed for life
- Stainless steel breaker caps that resist damage
- 3 epoxy-coated shelves per section, adjustable for convenience
- Automatic non-electric condensate evaporator that saves energy
- (4) 6-in. high casters with locks for mobility
- Microprocessor control system is easy to use and water-resistant
- Snap-in door gaskets are magnetic for a tight seal
- Anodized aluminum door liner protects gaskets
- One-piece louver assembly for easy maintenance
- 115 V/60 Hz/1-ph, 9-ft. cord and one NEMA 5-15 P plug provided
- Overall Dimensions: 52.125-in. W x 35-in. D x 83.25-in. H
- cULus, NSF, and ENERGY STAR listed
Dealer's Choice Refrigerator, Reach-in, two-section, self-contained refrigeration with microprocessor control, stainless steel front & full height doors (hinged left/right), anodized aluminum sides & interior, (3) epoxy coated shelves per section (factory installed), 6" high casters, 1/3 HP, cULus, NSF, ENERGY STAR
*The warranty will not be honored for residential or non-commercial use of any Commercial Equipment.
Traulsen G20010 SpecificationsPDF Spec Sheet
|Compressor Location
|Top Mount
|Cubic Feet
|Door Type
|Evaporator Type
|Expansion Valve
|Interior Material
|2 Section
|Thermometer Type
|3 year parts & labor; 5 year compressor
|Width (in)


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Police arrested a former Beech Grove schools wrestling coach after a short police chase.
Police arrested Nicholas Johnson Walpole, 25, 7536 Rooses Lane, Indianapolis, on Feb. 16 on two counts of resisting police and a charge of driving while intoxicated.
Walpole was an assistant coach and teaching assistant at Beech Grove High School. Superintendent Paul Kaiser said Walpole no longer is employed with the school corporation.
A Greenwood officer tried to stop a driver for speeding on Emerson Avenue. He continued driving, turned into a parking lot and ran his sport utility vehicle up a curb, according to the police report.
The driver got out of the car and tried to hide from police. Police used a Taser on him twice when he tried to run away, the report said.
Walpole was arrested and taken to the Johnson County jail, where he was released on $7,000 bond.
The Johnson County prosecutor will decide what, if any, charges to file.


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MS Office
Security methods News
March 12, 2015
Banking Trojans decline, but financial malware threat persists
A new Symantec report shows a significant fall in banking malware infections in 2014, but the company warns that malware remains a threat to financial firms.
February 06, 2015
Budget, breach law highlight growing federal cybersecurity awareness
News roundup: With the proposed 2016 federal budget and push for a national data breach law, Washington may finally care about cybersecurity. Plus: Coviello to retire; Flash patched again; Sony Pictures breached by Russians and loses its co-chair.
December 19, 2014
Active defense: The perils of cybervigilantism
Legally ambiguous active defense strategies are risky -- and costly -- for businesses, but could the benefits outweigh the drawbacks? In this #CIOChat recap, participants highlight the hazards of hacking back.
December 17, 2014
The future of active cyberdefense
Cyberthreats are becoming more advanced, with more companies turning to offensive security tactics to combat them. #GRCChat-ters predict how companies can navigate the unclear waters of active cyberdefense.
Security methods Get Started
Bring yourself up to speed with our introductory content
Ransomware protection is futile, but all hope is not lost
It's only a matter of time before a hacker infiltrates your system and holds your files hostage. But there are ways to blunt a Windows ransomware assault. Continue Reading
What are the most important security testing basics?
Knowing how to test for security flaws is vital, but it's a complicated and changing field. Expert Kevin Beaver offers security testing basics. Continue Reading
What's the difference between extortionware and ransomware?
Enterprise threats expert Nick Lewis explains the difference between extortionware and ransomware in terms of what they are and how to defend against them. Continue Reading
Evaluate Security methods Vendors & Products
Weigh the pros and cons of technologies, products and projects you are considering.
Why a cap-less cybersecurity budget could harm security
An unlimited cybersecurity budget may sound like a dream, but in reality it could do more harm than good for an enterprise. Expert Mike O. Villegas explains. Continue Reading
How can vaultless tokenization protect data in the cloud?
How do vaultless tokenization and standard tokenization differ, and what is the best way to use them for securing cloud data? Expert Dan Sullivan offers guidance and use cases. Continue Reading
Taming today's cyberthreat landscape: A CIO checklist
The cyberthreat landscape grows more dangerous by the day. Harvey Koeppel offers a 12-point cybersecurity checklist for CIOs. Continue Reading
Manage Security methods
Learn to apply best practices and optimize your operations.
How to use the ELK stack log monitoring tool
Open source log monitoring tools are a budget-friendly way for organizations to increase network visibility and improve incident response times. Continue Reading
Focus VDI security plans on malware prevention
Don't underestimate the security hazards associated with desktop virtualization. IT shops still need to protect all aspects of their VDI environment against malware. Continue Reading
Advanced malware detection is crucial to enterprise defense
Advanced malware is stealthy and deadly. Learn how defend your network and data against it with tools that provide advanced malware detection capabilities. Continue Reading
Problem Solve Security methods Issues
We've gathered up expert advice and tips from professionals like you so that the answers you need are always available.
Can thinking like cyberattackers improve organizations' security?
Getting in the minds of cyberattackers can help organizations mount better defenses against attacks. Here are some ways to accomplish this. Continue Reading
Offensive countermeasures: How they can slow down adversaries
Sometimes the best defense is a good offense. Expert Eric Cole explains the merits of offensive countermeasures in the enterprise. Continue Reading
In denial about DDoS: Defense planning falls short
Advanced distributed denial-of-service attackers are using a mix of techniques to hit targeted victims in ways all too similar to advanced persistent threats. DDoS defense planning is still lax at many enterprises, but if you do not have mitigation processes in place, it will cost you. Response, brand reputation and opportunity costs quickly add up in major attacks. The primary cost for victims, tied to the length of the outage, is revenue interruption says John Pescatore, director of emerging trends at the SANS Institute. The former Gartner analyst looks at the current denial-of-service landscape and associated costs in his article, "DDoS defense planning falls short." Adam Rice and James Ringold continue their series on APTs. In their article "Man versus machine data," the authors outline strategies to implement verbose logging across the enterprise and discuss the promise of big data security analytics to help security professionals track the APT lifecycle. The hard issues around intellectual property protection and the steps enterprises and vendors are taking to address them are also covered this month in Feature Editor Kathleen Richards' article, "Breaches reignite intellectual property protection." We also welcome back longtime columnist Marcus Ranum, who starts off the year chatting about metrics with IBM's Diana Kelley. Security 7 winner Frederick P. Chang, Ph.D., offers his take on what is needed to take cybersecurity education and science to the next level. Continue Reading


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Most Downloads
Fiction » Adventure » Action
John Smith, World Jumper Book One: Portal to Adventure by E. Patrick Dorris
Price: Free! Words: 63,930. Language: English. Published: November 4, 2008. Categories: Fiction » Science fiction » Adventure, Fiction » Adventure » Action(4.27 from 15 reviews)
Waking, hospitalized with amnesia during World War I, "John Smith" quickly realizes he is not like other men. John can travel to parallel worlds, seemingly through the power of his own mind. In this first serial, he not only learns of his power when it is triggered in the stress of combat, but visits an Earth still trapped in an ice age.
La morte è soltanto il principio by Rita Carla Francesca Monticelli
Price: Free! Words: 53,600. Language: Italian. Published: March 2, 2012. Categories: Fiction » Adventure » Action, Fiction » Fantasy » Historical(5.00 from 1 review)
1926 d.C., Lord Robert MacElister muore in circostanze misteriose durante una campagna di scavi in Egitto. Di ritorno a Londra, la giovane vedova porta con sé una grande quantità di reperti da esporre al British Museum e uno strano egiziano di nome Assad, indossante il tatuaggio dei Med-Jai, antichi guardiani di Hamunaptra... la Città dei Morti scoperta da Evelyn, John e Rick.
Amanda Ackers and The Realm Of The Witches by GlennAndSasha Gabriel
Series: Amanda Ackers Adventure, Book 2. Price: Free! Words: 180,810. Language: English. Published: March 28, 2012. Categories: Fiction » Fantasy » General, Fiction » Adventure » Action(4.94 from 18 reviews)
Book Two of the Series. Fleeing for their lives after Amanda turns the wizard Blaine into stone, then fighting for her life in other battles, Amanda and her friends transport to The Realm of The Witches, in search of the mighty witch Bellinora - the only one who may be able to save Amanda. Will they survive the fierce battles against witches, wizards and hideous creatures that await them?
Amanda Ackers and The Deep Forest Elves by GlennAndSasha Gabriel
Series: Amanda Ackers Adventure, Book 1. Price: Free! Words: 244,300. Language: English. Published: January 3, 2012. Categories: Fiction » Fantasy » General, Fiction » Adventure » Action(4.52 from 25 reviews)
Book One. This series tells of the Greatest Adventures In Modern Elvish History. Challenges and battles abound. Join Amanda Ackers and elves, dwarves, witches, wizards, and other creatures, as she finds a long lost and powerfully Wizitched artifact, that she was never meant to find. She must learn to control the spirits trapped within it, or all the good within all realms, will perish.
John Gone by Michael Kayatta
Price: Free! Words: 100,820. Language: English. Published: February 25, 2013. Categories: Fiction » Science fiction » Adventure, Fiction » Adventure » Action(3.80 from 5 reviews)
After finding a mysterious wristwatch buried in the sand, sixteen-year-old John suddenly finds himself teleporting farther and farther away from his home each day when the hands strike 3:14. Unable to deactivate the device or remove it from his arm, John must do what he can to survive when its dangerous creator comes looking for his lost invention. John Gone is Book One of The Diaspora Trilogy.
The Caves of Etretat: Part One of Four by Matt Chatelain
Series: The Caves of Etretat, Book 1. Price: Free! Words: 108,090. Language: English. Published: April 25, 2012. Categories: Fiction » Thriller & suspense » Action & suspense, Fiction » Adventure » Action(5.00 from 4 reviews)
New version Jan 21 2016. Canadian bookstore owner Paul Sirenne heads to the town of Etretat, France, on the trail of a hundred year old mystery hidden in the pages of 'The Hollow Needle', by Maurice Leblanc. Together with Leblanc's great-granddaughter, Sirenne unearths puzzles, codes and historical mysteries, exposing a secret war for control of a cave fortress in Etretat's chalk cliffs.
Night of the Assassin by Russell Blake
Series: Assassin, Book 1. Price: Free! Words: 60,250. Language: English. Published: December 12, 2011. Categories: Fiction » Thriller & suspense » Action & suspense, Fiction » Adventure » Action(4.50 from 4 reviews)
The prequel to King of Swords, Night of the Assassin charts the early life of El Rey, the super-assassin who specializes in impossible-to-execute sanctions on behalf of his brutal narco-trafficking cartel clients. Night of the Assassin is a glimpse into the making of the monster, and is an intense, no-flinching, gritty epic that will leave even the most jaded thriller fan gasping.
Agent with a History by Guy S. Stanton III
Series: The Agency for Good, Book 1. Price: Free! Words: 59,490. Language: American English. Published: October 26, 2013. Categories: Fiction » Adventure » Action, Fiction » Romance » Contemporary(4.60 from 5 reviews)
It's been one long day for Lisa and her headache is living proof of it. Things always got crazy this time of the month, but this month was exceptional. She needed sleep bad and only hoped the nightmares that haunted her would leave her alone for at least one night. They don't. She wakes up as usual screaming only to find out that her nightmare has just begun, because she's not alone in her room.
Murderous Relations by Anna Drake
Series: Murderous Tales. Price: $2.99 USD. Words: 62,240. Language: English. Published: November 16, 2009. Categories: Fiction » Christian » Mystery & detective, Fiction » Adventure » Action(3.60 from 5 reviews)
A lost love and a murdered aunt push a shy teacher out of her shell and straight into trouble. The detectives on her aunt's case see Jessica as the possible killer. Her aunt's friend thinks Jessica and she should track the murderer down themselves. Meanwhile the killer sees Jessica as a serious risk to his freedom. He waits and watches, ready to strike if he must.
Quala - The Plush Planetary Takeover by Mark Richmond
Price: Free! Words: 9,970. Language: English. Published: August 2, 2010. Categories: Fiction » Adventure » Action, Fiction » Science fiction » Adventure
Armed only with squirt guns and water balloons, A 10 year old, orphan girl, and her Artificially Intelligent toy Koala, take on thousands of rogue Ai toys, deceitful humans, and gods to save the universe.
The Deadliest Menace by V Bertolaccini
Price: $4.95 USD. Words: 56,060. Language: English. Published: September 15, 2011 by CosmicBlueCB. Categories: Fiction » Thriller & suspense » Crime thriller, Fiction » Adventure » Action
A new proficiently intellectual action thriller that merges a contemporary global terrorist scenario with a fascinating collection of episodes. The execution of an atomic explosion on a South Pacific military base, the assassination of a leading UK politician, and the hijacking of a new space shuttle reveals someone has acquired plutonium and is executing the deadliest ever plot on civilization.
The Four Books of Etretat: Part Two of Four by Matt Chatelain
Series: The Caves of Etretat, Book 2. Price: $2.99 USD. Words: 96,610. Language: English. Published: May 5, 2012. Categories: Fiction » Thriller & suspense » Action & suspense, Fiction » Adventure » Action(5.00 from 1 review)
In Book 1, Canadian bookstore owner Paul Sirenne exposed a secret war for a fortress in Etretat's chalk cliffs. In Book 2, Sirenne discovers the reason for the hidden war: the secret of immortality. The Abbey, a thousand-year old organization, is preparing Sirenne for a confrontation against the immortal Greyman. Unfortunately, the serial-killer Weissmuller seems to be playing a game of his own.
Engraved on the Eye by Saladin Ahmed
Price: Free! Words: 38,510. Language: English. Published: September 13, 2012 by Ridan Publishing. Categories: Fiction » Fantasy » Short stories, Fiction » Adventure » Action(4.00 from 2 reviews)
A medieval physician asked to do the impossible. A gun slinging Muslim wizard in the old West. A disgruntled super villain pining for prison reform. A cybernetic soldier who might or might not be receiving messages from God. These short stories have been nominated for the Nebula and Campbell awards, and reprinted in The Year's Best Fantasy Anthologies.
Jet by Russell Blake
Series: JET, Book 3. Price: Free! Words: 81,860. Language: English. Published: December 23, 2012. Categories: Fiction » Adventure » Action, Fiction » Thriller & suspense » Action & suspense(4.89 from 9 reviews)
Code name: Jet Twenty-eight-year-old Jet was once the Mossad's most lethal operative before faking her own death and burying that identity forever. But the past doesn't give up on its secrets easily. When her new life on a tranquil island is shattered by a brutal attack, Jet must return to a clandestine existence of savagery and deception to save herself and those she loves.
Flight by Neil Hetzner
Price: Free! Words: 160,250. Language: English. Published: May 4, 2012. Categories: Fiction » Adventure » Action, Fiction » Science fiction » Adventure(4.50 from 2 reviews)
It's 2097 and teens can fledge and grow wings. Fifteen year old Prissi Langue loves flying, school and her friends. However, after Prissi finds pictures of her dead mother with the man who invented fledging, she begins an investigation that uncovers long hidden secrets. Suddenly, it seems like everyone wants Prissi dead. Can Prissi fly far enough and fast enough to escape her enemies?
Retribution by Nicholas Gill
Series: Fine Line Solutions, Book 1. Price: Free! Words: 133,250. Language: American English. Published: August 14, 2012. Categories: Fiction » Thriller & suspense » Action & suspense, Fiction » Adventure » Action(5.00 from 6 reviews)
A small survivor of a brutal massacre becomes the leader of a terrorist splinter group intent on attacking soft targets in the west. The novel develops into a detective story to find the brains behind the attacks and into a clandestine operation to exact "retribution" for the evil done, but the terrorist agenda is not complete, a much larger plot with grave effects for the western nations begins.
The Grin of Prophecy (Book 1 of the Death Incarnate Saga) by H. Lee Morgan, Jr
Series: The Death Incarnate Saga, Book 1. Price: Free! Words: 187,500. Language: English. Published: August 15, 2013. Categories: Fiction » Adventure » Action, Fiction » Fantasy » Epic(3.83 from 6 reviews)
On the run from exacting vengeance on a traitorous general for the loss of his team that was betrayed, Cage soon finds himself unable to escape. Then out of nowhere he is sucked to another world.
Henri Ville by M. Chris Benner
Price: $1.00 USD. Words: 73,430. Language: English. Published: September 17, 2012. Categories: Fiction » Adventure » Action, Fiction » Historical » Western & American frontier(5.00 from 3 reviews)
Relentlessly pursued by crooked marshals, idiot bounty hunters, and a psychotic pastor, infamous gunfighter Henri Ville takes the first steps in her epic journey to return home.
Trouble and Treasure (#1, Trouble and Treasure Series) by Odette C. Bell
Series: Trouble and Treasure, Book 1. Price: Free! Words: 67,930. Language: English. Published: July 16, 2012. Categories: Fiction » Adventure » Action(5.00 from 2 reviews)
An action adventure packed with danger, mystery, and just enough romance, the Trouble and Treasure series follows the adventures of Amanda and Sebastian as they try to track down one of the most valuable treasure maps in the world.
Sliding Void (Book 1 of the Sliding Void science fiction series) by Stephen Hunt
Price: Free! Words: 31,470. Language: English. Published: July 22, 2012. Categories: Fiction » Science fiction » Space opera, Fiction » Adventure » Action(5.00 from 3 reviews)
Captain Lana Fiveworlds has a hell of a lot of problems. She's sliding void in an ageing seven-hundred-year-old space ship, scrabbling around the edges of civilised space trying to find a cargo lucrative enough to pay her bills without proving so risky that it'll kill her.
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