text:
Update: We've now written our full and in-depth Canon EOS 550D review
Canon unveiled its latest DSLR to shoot Full HD video, the Canon EOS 550D, at a swanky event in London this week and TechRadar were on hand for a touchy feely session with the camera.
Acting like an upgraded 500D, the camera totes a bigger, better sensor in the guise of the APS-C CMOS. Essentially this means brighter images due to the amount of light which hits the sensor.
The chassis is, like the 500D, nice and lightweight – great for those who are fed up with the cumbersome size of some DSLRs on the market.
Those familiar with the 500D will feel right at home with the 550D – the enlarged buttons on the back of the camera make another appearance, including a few other option designed to bring the movie elements of the camera to the fore.
One of these is the Movie Crop function which is good for those who can't quite get close enough to the action.
We would never recommend cropping too much into a movie frame but the results we got were more than passable.
Back to the camera elements of the 550D, there's a 9-point AF system on-board which locked us on to subjects nice and fast and the ability to expand, with a bit of trickery, the ISO to 12800.
It's the movie aspect of the camera which is standout in the 550D, however; something which Canon is heavily promoting.
This was originally seen in the invitation to the launch which was titled 'Canon At The Movies' and the press conference took place at BAFTA's prestigious headquarters in Piccadilly, London.
The filmmaking market is one which Canon eagerly wants to snap up and one which it already semi-controls with its mix of prosumers and professional film cameras. Such a pedigree then means that if you were to treat the EOS 550D as nothing but a movie making machine, you wouldn't be disappointed.
Canon has certainly upped the ante when it comes to filming modes. Now there's a choice between 30, 25 and 24fps (on the Full HD option) and 60 and 50fps (on 720p). This patches up a major bug-bear users had with the 500D's limited filming options which were poor for fast-moving images.
There's one caveat that the Canon and the rest of the DSLR crowd has to overcome, though. Due to tax restrictions in the UK, filmmaking on the DSLR is confined to around 12 minutes for a take – any more and the device would be subject to import restrictions.
This isn't a major problem, unless your movies resemble Hitchcock's Rope but something to bear in mind.
This does bring home the fact that what you are using is a camera first and a camcorder second. Saying that, the lines are certainly blurring with the 550D which is sure to pique the interest of photographers and videographers alike.
The Canon EOS 550D has a UK release date of 24 February. Price-wise, the camera will be available body-only for a penny under £799.99.
To show off the camera, Canon has released a promo video for the EOD 550D which we have posted below.
Article continues below


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Battery is good! When you turn the key there is nothing-no panel lights and you can hear a very rapid clicking sound coming from under the steering wheel near the fuses and relays. have checked the fuses and all seem to be fine. WHATS UP??
by haatfinn in Sidney, MI on March 21, 2009

subset: commoncrawl


text:
An unboxing of the innovative Samsung Galaxy Camera. A mix of smartphone and compact camera running Google Android operating system yet this device can not make phone calls like a conventional smartphone. For more information on product shown pleas...
SMARTPHONES Reviews
Huawei Honor 4X review: Battery life is good, but there are better options under Rs 10,000
Lenovo A6000 review: A good entry level phone with a great display but average camera
HTC Desire 526G+ Review: A good budget smartphone, but competition has the price advantage
May 30, 2016
May 30, 2016
May 30, 2016
NOTEBOOKS Reviews
Asus EeeBook X205TA review: For students on a budget; but low storage and no USB 3.0 port are downers
Asus ROG G551JK review: At Rs 82,999 offers a good package, but there is scope for improvement


text:
The Russian Federation if one of the largest suppliers of wild marine mammals to marine parks around the world and has several of its own captive dolphin and whale facilities.
With an ever expanding market in the Far East, especially China, there is continued demand for cetaceans, especially beluga whales and orcas. Each year Russia are given a capture quota allowing animals to be taken from the wild "for scientific, cultural and educational purposes" and Russia takes full advantage of this quota, and more! And it's not just the Far East market who are showing interest. A US facility, representing a breeding consortium of five other public display facilities including the three SeaWorld parks, applied to import 18 beluga whales which were being held in Russia as part of their live-capture and beluga fisheries programme. To date, this import has not been authorised.
There are plans for yet more facilities in Russia featuring captive dolphins and whales and yet still there are no laws in place to protect the welfare of these animals – despite the fact that this country still has travelling dolphins/whale shows (currently there are around 10 travelling shows in total.) The animals are moved from city to city in trucks, spending days in tiny tanks with no ability to move around – touring for sometimes up to 6 weeks at a time. Many die en-route.
In the wild orcas can live for decades, in captivity they may not even reach 10 years of age. This continued demand means that, certainly in Russia, even more cetaceans will be sentenced to a life in captivity, confined to barren tanks, devoid of stimulation and face a shortened lifespan, all for public entertainment.


text:
Forster backs Saints in Europa campaign
Southampton keeper Fraser Forster says they have the quality to mount a strong Europa League challenge next season.BBC Sport » 51m
Southampton keeper Fraser Forster says they have the quality to mount a strong Europa League challenge next season.BBC Sport » 51m
Mesut Ozil is happy at Arsenal and will discuss a new contract with the club after Euro 2016.SkySports.com » 1h
Connor Randall admits Liverpool's Wembley friendly against Barcelona in August is a huge incentive for Reds youngsters to impress boss Jurgen Klopp when they report back for pre-season training.SkySports.com » 1h
Middlesbrough are set to complete the signing of Ajax winger Viktor Fischer on Thursday, Sky sources understand.SkySports.com » 1h
Cristiano Ronaldo says Jose Mourinho can restore Manchester United's "identity" when he takes over - but insists he won't be joining his fellow Portuguese at Old Trafford.SkySports.com » 2h
Bayern Munich say Mario Gotze will have to decide for himself whether he wants to move away from the club.SkySports.com » 2h
Everton striker Romelu Lukaku has revealed that he wants to leave the club over the summer in order to further his career.SkySports.com » 3h
Striker Mario Balotelli appears likely to make a return to Liverpool from AC Milan for pre-season training.SkySports.com » 3h
Our history pages are complete for English, Scottish & Welsh leagues, all cups and international competitions.
For all other leagues, we show the recent history.
|Final Details


text:
Jackson County, FL - In the month of January, 2010 a burglary occurred on Neel Road in the Grand Ridge, Florida area. The victim reported that the rear door of his residence was found kicked open and several items were stolen. Among the items listed as stolen was a .44 caliber revolver.
During the month of June, 2011 the Jackson County Sheriff's Office discovered that the revolver had been sold at a local Pawn Shop.
Investigators learned that the revolver had been sold by Timothy Eugene Davis.The investigation revealed that Timothy Eugene Davis and his brother David Wayne Davis had commited the burglary and sold the stolen property at multiple area pawn shops.
Timothy Eugene Davis was arrested and charged with armed burglary of a dwelling and grand theft. David Wayne Davis was arrested and charged with being a principal to armed burglary, principal to grand theft and dealing in stolen property.
The investigation is ongoing and further charges are pending. Anyone with information regarding this incident is asked to contact the Jackson County Sheriff's Office's Criminal Investigation Division at (850)482-9664.


text:
Julio Iglesias
Tour Dates 2016, 2017
Julio Iglesias concert tickets are on sale. You can find the list of Julio Iglesias tour dates here.
Julio Iglesias is a Spanish singer and songwriter who has sold over 300 million records worldwide in 14 languages and released 80 albums, and more than 2,600 gold and platinum records certified. According to Sony Music Entertainment, he is the best-selling Latin music artist in history.
Questions about Julio Iglesias Tour?
Julio Iglesias Past Concerts
Julio Iglesias New Album
Romantic Classics (2006)
- Everybody's Talking
- How Can You Mend a Broken Heart?
- Careless Whisper
- Always on My Mind
- Waiting for a Girl Like You
- Drive
- I Want to Know What Love Is
- Right Here Waiting
- This Guy's in Love With You
- The Most Beautiful Girl
- It's Impossible
tour dates, concerts, concert ticket, tour dates 2016, concert dates, ticket
Tour Dates
tour dates , concerts , concert ticket


text:
Images: A prayer breakfast with character [Image 3 of 10]
Photo by Sgt. 1st Class E. L. Craig
U.S. Army Sgt. Eliza Balisacan listens during the first Forward Operating Base Gamberi prayer breakfast Dec. 6, 2013. Balisacan, a native of the Philippines, serves as the supply noncommissioned officer in charge for Headquarters and Headquarters Company, 4th Brigade Combat Team, 10th Mountain Division. The prayer breakfast's topic was the "Character of a Leader." (U.S. Army Photo by Sgt. 1st Class E. L. Craig, Task Force Patriot PAO)
This work, A prayer breakfast with character [Image 1 of 10], by SFC E. L. Craig, identified by DVIDS, is free of known copyright restrictions under U.S. copyright law.
Date Taken:12.06.2013
Date Posted:12.08.2013 10:08
Photo ID:1067891
Size:877.23 KB
Location:FORWARD OPERATING BASE GAMBERI, AF
Gallery Images
Associated News
A prayer breakfast with character


text:
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text:
SAO PAULO With 1 billion posts, likes and comments in just the first half of the World Cup, the soccer tournament is already the most talked-about event in Facebook Inc's decade-long history, data obtained by Reuters showed.
The soccer conversation measured between June 12 and June 29 involved 220 million people and 1 billion interactions, the Facebook data showed. And since the ball will be rolling for another two weeks, the tournament is set to break new records as the biggest social media event to date.
"People are having conversations on Facebook about what they watch in a really unprecedented scale," Nick Grudin, the company's director of partnerships, told Reuters.
"In addition to sharing and connecting with friends, people are engaging in real time with the media and the public voices they care about most."
Facebook is the latest social media company to capitalize on TV-related traffic around big events like the World Cup, a trend started years ago by the microblog website Twitter Inc.
People use Facebook to comment about things they watch live, an interaction that could turn into a source of ad revenue for the company.
Facebook's record numbers were possible because of widespread mobile penetration. Seven out of 10 users globally connect to the network from mobile devices, which represent roughly 60 percent of the company's ad revenue.
There is also soccer's global appeal. The first week of the World Cup alone saw 459 million interactions on Facebook, more than this year's Super Bowl, the Sochi Winter Olympic Games and the Academy Awards combined.
The 1 billion mark was reached after traffic accelerated as the World Cup moved into the knockout round. On Saturday, more than 31 million people put up 75 million posts, likes and comments about Brazil's nail-biting victory over Chile, which propelled the home team to the quarter-finals.
"This Cup has been a catalyzing cultural moment for people around the world," Grudin said, "and we see it reflected on Facebook."
(Editing by David Holmes and Lisa Von Ahn)
Trending On Reuters
Radwanska suffers Paris meltdown in defeat by Pironkova
World number two Agnieszka Radwanska suffered a monumental meltdown at a soggy French Open as her Roland Garros hopes fizzled out with a 2-6 6-3 6-3 defeat by Bulgarian Tsvetana Pironkova in the fourth round. Story
Reuters Showcase
Reuters India App
Download the Reuters app from the Apple App Store, Google Play and Amazon. Select the India edition from settings. Full Coverage


text:
Stimol: The crown of thorns
Sport without pain
When somebody goes in for sports seriously, he learns the proverb "No pain – no gain". With Stimol pain stops being an indication of toughness.
Advertising Agency: Rosst, Moscow, Russia
Creative Director: Andrey Kiselev
Art Director: Emilian Olaru
Copywriter: Oleg Malikov
Illustrator: New Folder
Photographer: Dan Vezentan
Additional credits: New Folder
Published:September 2013


text:
ELD : Analysis & Opinions
3 Best High-Yielding Blended Bond ETFs (ELD, EMAG)May 3, 2016Learn how blended bond exchange-traded funds (ETFs) mitigate risk by incorporating multiple fixed-income strategies within ...
Emerging Market Bond ETFs: Look Under The HoodSeptember 29, 2014Interested in diversifying your portfolio with some emerging market debt? Have a look at these key ETFs.
Invest In Emerging Market Bonds With These ETFsSeptember 11, 2014With the variety of emerging market bond ETFs now on the books, it's become far easier for investors to diversify. Here's ...
The Better Bet: Emerging Market Debt Or Equity?May 15, 2014Want the returns of emerging markets with less of the volatility and risk? Have a close look at corporate and sovereign debt ...
Market Vectors Completes Fund Conversion: EM Aggregate Bond ETF ...December 12, 2013Equity markets continue to oscillate this week as an upward bias mixed with uncertainty ahead of the highly anticipated FOMC ...
The Fed Gives The OK To Load Up On Emerging MarketsSeptember 24, 2013Continued quantitative easing by the Fed suggests emerging markets may still be a buy. Here are some ETFs to consider.
Find Income and Growth In Emerging Market BondsApril 10, 2013Offering both growth and income potential, emerging market debt could be one of the best plays for your portfolio.
Emerging Markets Bonds ETF Cheat SheetJanuary 17, 2013As the U.S. market slowly wanders towards the road to recovery, many investors who had avoided the short term effects of ...
The Attractive Play In Emerging Market BondsJanuary 10, 2012The strong fiscal conservatism of emerging market bonds makes them an ideal candidate for a fixed income portfolio.
Spice Up A Bond Portfolio By Going GlobalSeptember 30, 2011In order to get extra income, investors may want to open their portfolios up to international bonds.
BlackRock's Bold ETF PlaySeptember 9, 2011BlackRock looks to launch ETFs that do not have to make daily disclosures.
Trading Center


text:
92,494 shares
I didn't get a great sleep last night.
12,613 shares
Socially awkward penguin strikes again!
12,096 shares
I should just drop out of college now...
12,494 shares
Oh God Why
12,532 shares
Guys I messed up bad...
12,446 shares
Remembers joke at urinal looks like I'm laughing at my own penis
19,073 shares
Reads SAP memes Identifies with most
15,892 shares
Tries to imagine interviewers naked gets boner
17,985 shares
Tells coworker she's lost weight as a compliment Miscarriage
292,278 shares
Person walking in front opens door for me 4 times in a row "Thanks, Thanks, Thanks, Thank You"
200,241 shares
Laugh more daily


text:
Hello all!
I recently bought an Florida Resident Annual Pass. I live in Jacksonville which really isn't very far from the Mouse at all and have relatives in Deltona which is even closer.
One of my uncles is a HUGE Disneyworld fan and has probably visited over 80 times. He's always taking his granddaughters and after a few tag alongs buying single day tickets, it just made more sense to get a pass.
I love going with family and friends however, I've recently started considering a multi-day solo trip. I'm into photography and it would be nice to take my time and take as many park pictures as I wanted without being hurried along to the next attraction.
I've also been thinking about springing for the Backstage Magic Tour for my uncle and myself. As many times as he's been, he's never done a tour and I think he'd love it.
Anyway, forums such as this are always a great source of information. I'm looking forward to future visits.
~Hoodat Whatzit


text:
'Jewel In The Palace' became popular in Cuba
Because Cubans have found out K-dramas, they can't enjoy all of the K-drama. At the beginning of last year, they first watched K-drama goodness with the comedy "Queen of Housewives" which was published in 2009 on the Canal Habana, winning ratings of 70 percent. After that, Cubans continued to see "My Fair Lady" which featured Yoon Eun Hye as the spoiled rich girl and Yoon Sang Hyun as her chauffer. The romantic comedy made Yoon Sang Hyun a sought-after star in Cuba. Next aired was "Secret Garden," the body swap comedy that stars Hyun Bin and Ha Ji Won. Now the historical drama "Jewel in The Palace," also known as "Dae Jang Geum" is airing.
Censors are currently reviewing "Boys Over Flowers," which stars Lee Min Ho. But many Cubans are not waiting for the official review. Street vendors are selling about 60 different pirated as yet unrated k-drama DVDs. Dramas with Lee Min Ho are high in demand.
The dramas were provided for free by the Korea Trade Investment Promotion Agency (KOTRA) and they have done their part in promoting the image of Korea on the island nation. Oh Young Ho, KOTRA"s CEO received an award in Cuba on Nov. 4 for his role in promoting economic and cultural exchanges between Korea and Cuba.


text:
Feel like home at ringtone download service Audiko.net, where you can search for the best ringtones for your iPhone. Today we have a ringtone of "Du schreibst Geschichte" by Madsen as well as 81 other ringtones by Madsen. "Du schreibst Geschichte" is one of the best tracks from the "Goodbye Logik" album that was recorded by Madsen at their label together with other 5 tracks. You can listen to all 7 Madsen – "Du schreibst Geschichte" ringtone variations available here and download it for free to your PC. You can also listen to other 5 albums produced by Madsen for all time. "Du schreibst Geschichte" is referred to German, and we have a huge amount of German artists absolutely for free. And finally, we have collected lyrics for this track and you can view it here as well. So feel free to browse, listen, download ringtones at Audiko.net. Make your phone sound cool!
Madsen Du schreibst Geschichte Ringtone
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text:
Diskets - Is methadone a opiate blocker?
- Posted:
- 27 Sep 2011 by mudduck223
- Topics:
- diskets, methadone, opiate
Answers (1)
28 Sep 2011
No. Methadone is an opiate similar to morphine. It is used to prevent withdrawal symptoms in those trying to get off heroin and other narcotics. It is also used as a pain reliever.
Further Information
- Methadone Information for Consumers
- Methadone Information for Healthcare Professionals (includes dosage details)
- Side Effects of Methadone (detailed)
Search for questions
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... least 7-10 r's and if I can get it 4-5 opanas a day just to function. I am trying to take methadone and zanis to detox. Last night I took ...
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text:
------Bernina 630, Bernina embroidery module, Pfaff 2036, Bernina 1200DA serger, Unique Sewing Cabinet 450L
This one, that one, and a bunch of other ones!
(though the herd is getting more streamlined)
------"TOL machines = Too (much) Operator Learning"--poorpigling, January, 2016
------Bernina 560, Bernina 230 White Pearl, Pfaff 7550, Pfaff 1171, Babylock Imagine, Babylock Coverstitch
------🌸 Plenty of machines, mostly Berninas 🌸
------"Improvise, adapt and overcome." - Clint Eastwood/Heartbreak Ridge
"Isn�t it a shame in today�s world you can�t tell the truth. If you tell the truth you�re abrasive. If you lie you�re charming." - Bob Huggins/College Basketball Coach
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text:
Eric Davidson, Caltech's Norman Chandler
Professor of Cell Biology, has been awarded the 2011 International
Prize for Biology by the Japan Society for the Promotion of
Science. On November 28, Davidson received a medal at a ceremony in
Tokyo and an imperial gift, a silver vase from Emperor Akihito. The
award also includes ten million yen (more than $125,000 USD).
Davidson was recognized for pioneering the
concept of gene regulatory networks, which are networks of hundreds
of interacting regulatory genes that control the development of an
animal from embryo to adult. Regulatory genes are segments of DNA
that determine when and where other genes are turned on or off.
Ultimately, these regulatory networks dictate the expression of all
the genes that encode for the proteins responsible for an animal's
biological structure and function. Each year's International Prize
for Biology is awarded in a different area of biology; this year,
the prize recognized achievements in developmental biology.
"Eric has been a true pioneer in recognizing
that complexity is a fundamental attribute of biological systems,"
says Stephen Mayo, the Bren Professor of Biology and Chemistry at
Caltech, and chair of the Division of Biology. "His recognition for
achievements in developmental biology is both timely and well
Davidson is the fourth winner from Caltech
since the inception of the prize in 1985. Previous Caltech
recipients are Masakazu Konishi, the Bing Professor of Behavioral
Biology; Elliot Meyerowitz, the George Beadle Professor of Biology;
and the late Seymour Benzer.
The prize was established to commemorate the
60-year reign of Emperor Akihito's late father, Hirohito, who was
long interested in biological research. Akihito himself has
published papers on fish evolution, and his son, Prince Fumihito,
has a PhD in evolutionary bioscience. Crown Prince Naruhito
attended the ceremony and was joined by Empress Michiko for the
reception. A two-day symposium in Kyoto, called "Genetic Control of
Development," followed the ceremony. "The week was an unforgettable
experience," Davidson says.
PASADENA, Calif.—Over the past year, researchers at the California Institute of Technology (Caltech), and around the world, have been studying a group of potent antibodies that have the ability to neutralize HIV in the lab; their hope is that they may learn how to create a vaccine that makes antibodies with similar properties. Now, biologists at Caltech led by Nobel Laureate David Baltimore, president emeritus and Robert Andrews Millikan Professor of Biology, have taken one step closer to that goal: they have developed a way to deliver these antibodies to mice and, in so doing, have effectively protected them from HIV infection.
This new approach to HIV prevention—called Vectored ImmunoProphylaxis, or VIP—is outlined in the November 30 advance online publication of the journal Nature.
Traditional efforts to develop a vaccine against HIV have been centered on designing substances that provoke an effective immune response—either in the form of antibodies to block infection or T cells that attack infected cells. With VIP, protective antibodies are being provided up front.
"VIP has a similar effect to a vaccine, but without ever calling on the immune system to do any of the work," says Alejandro Balazs, lead author of the study and a postdoctoral scholar in Baltimore's lab. "Normally, you put an antigen or killed bacteria or something into the body, and the immune system figures out how to make an antibody against it. We've taken that whole part out of the equation."
Because mice are not sensitive to HIV, the researchers used specialized mice carrying human immune cells that are able to grow HIV. They utilized an adeno-associated virus (AAV)—a small, harmless virus that has been useful in gene-therapy trials—as a carrier to deliver genes that are able to specify antibody production. The AAV was injected into the leg muscle of mice, and the muscle cells then put broadly neutralizing antibodies into the animals' circulatory systems. After just a single AAV injection, the mice produced high concentrations of these antibodies for the rest of their lives, as shown by intermittent sampling of their blood. Remarkably, these antibodies protected the mice from infection when the researchers exposed them to HIV intravenously.
The team points out that the leap from mice to humans is large—the fact that the approach works in mice does not necessarily mean it will be successful in humans. Still, the researchers believe that the large amounts of antibodies that the mice were able to produce—coupled with the finding that a relatively small amount of antibody has proved protective in the mice—may translate into human protection against HIV infection.
"We're not promising that we've actually solved the human problem," says Baltimore. "But the evidence for prevention in these mice is very clear."
The paper also notes that in the mouse model, VIP worked even in the face of increased exposure to HIV. To test the efficacy of the antibody, the researchers started with a virus dose of one nanogram, which was enough to infect the majority of the mice who received it. When they saw that the mice given VIP could withstand that dose, they continued to bump it up until they were challenging them with 125 nanograms of virus.
"We expected that at some dose, the antibodies would fail to protect the mice, but it never did—even when we gave mice 100 times more HIV than would be needed to infect 7 out of 8 mice," says Balazs. "All of the exposures in this work were significantly larger than a human being would be likely to encounter."
He points out that this outcome likely had more to do with the properties of the antibody that was tested than the method, but adds that VIP is what enabled the large amount of this powerful antibody to circulate through the mice and fight the virus. Furthermore, VIP is a platform technique, meaning that as more potent neutralizing antibodies are isolated or developed for HIV or other infectious organisms, they can also be delivered using this method.
"If humans are like mice, then we have devised a way to protect against the transmission of HIV from person to person," says Baltimore. "But that is a huge if, and so the next step is to try to find out whether humans behave like mice."
He says the team is currently in the process of developing a plan to test their method in human clinical trials. The initial tests will ask whether the AAV vector can program the muscle of humans to make levels of antibody that would be expected to be protective against HIV.
"In typical vaccine studies, those inoculated usually mount an immune response—you just don't know if it's going to work to fight the virus," explains Balazs. "In this case, because we already know that the antibodies work, my opinion is that if we can induce production of sufficient antibody in people, then the odds that VIP will be successful are actually pretty high."
The study, "Antibody-based Protection Against HIV Infection by Vectored ImmunoProphylaxis," was funded by the Bill and Melinda Gates Foundation, the National Institutes of Health, and the Caltech-UCLA Joint Center for Translational Medicine. Caltech biology researchers Joyce Chen, Christin M. Hong, and Lili Yang also contributed to the paper, as well as Dinesh Rao, a hematologist from the University of California, Los Angeles.
PASADENA, Calif.—Although many mental illnesses are uniquely human, animals sometimes exhibit abnormal behaviors similar to those seen in humans with psychological disorders. Such behaviors are called endophenotypes. Now, researchers at the California Institute of Technology (Caltech) have found that mice lacking a gene that encodes a particular protein found in the synapses of the brain display a number of endophenotypes associated with schizophrenia and autism spectrum disorders.
The new findings appear in a recent issue of The Journal of Neuroscience, with Mary Kennedy, the Allen and Lenabelle Davis Professor of Biology at Caltech, as the senior author.
The team created mutations in mice so that they were missing the gene for a protein called densin-180, which is abundant in the synapses of the brain, those electro-chemical connections between one neuron and another that enable the formation of networks between the brain's neurons. This protein sticks to and binds together several other proteins in a part of the neuron that's at the receiving end of a synapse and is called the postsynapse. "Our work indicates that densin-180 helps to hold together a key piece of regulatory machinery in the postsynaptic part of excitatory brain synapses," says Kennedy.
In mice lacking densin-180, the researchers found decreased amounts of some of the other regulatory proteins normally located in the postsynapse. Kennedy and her colleagues were especially intrigued by a marked decrease in the amount of a protein called DISC1. "A mutation that leads to loss of DISC1 function has been shown to predispose humans to development of schizophrenia and bipolar disorder," Kennedy says.
In the study, the researchers compared the behavior of typical mice with that of mice lacking densin. Those without densin displayed impaired short-term memory, hyperactivity in response to novel or stressful situations, a deficit of normal nest-building activity, and higher levels of anxiety. "Studies of mice with schizophrenia and autism-like features have reported similar behaviors," Kennedy notes.
"We do not know precisely how the molecular defect leads to the behavioral endophenotypes. That will be our work going forward," Kennedy says. "The molecular mechanistic links between a gene defect and defective behavior are complicated and, as yet, mostly unknown. Understanding them goes to the very heart of understanding brain function."
Indeed, she adds, the findings point to the need for a better understanding of the interactions that occur between proteins at synapses. Studies of these interactions could provide information needed to screen for new and better pharmaceuticals for the treatment of mental illnesses. "This study really reinforces the idea that small changes in the molecular structures at synapses are linked to major problems with behavior," Kennedy says.
Caltech coauthors of the paper, "Deletion of Densin-180 Results in Abnormal Behaviors Associated with Mental Illness and Reduces mGluR5 and DISC1 in the Postsynaptic Density Fraction," include Paul Patterson, the Anne P. and Benjamin F. Biaggini Professor of Biological Sciences; Holly Carlisle; Tinh Luong; Andrew Medina-Marino; Leslie Schenker; Eugenia Khorosheva; Keith Gunapala; and Andrew Steele. The paper's other authors are Tim Indersmitten and Thomas O'Dell of the David Geffen School of Medicine at the University of California, Los Angeles. The work was supported by the National Institutes of Health, the Gordon and Betty Moore Foundation Center for Integrative Study of Cell Biology, the Howard Hughes Medical Institute, the National Science Foundation, the McGrath Foundation, and the Broad Fellows in Brain Circuitry program.
PASADENA, Calif.—If the changing seasons are making it chilly inside your house, you might just turn the heater on. That's a reasonable response to a cold environment: switching to a toastier and more comfortable state until it warms up outside. And so it's no surprise that biologists have long thought cells would respond to their environment in a similar way.
But now researchers at the California Institute of Technology (Caltech) are finding that cells can respond using a new kind of pulsating mechanism, instead of just shifting from one steady state to another and staying there. The principles behind this process are surprisingly simple, the researchers say, and could drive other cellular processes, revealing more about how the cells—and ultimately life—work.
In their experiment, the researchers studied how a bacterial species called B. subtilis responds to a stressful environment—for example, one without food. In such conditions, the single-celled organism activates a large set of genes that help it deal with hardship, by aiding cell repair for instance. Previously, biologists had thought the bacteria would handle stress by turning on the relevant genes and simply leaving them on until the stress goes away.
Instead, the researchers found that B. subtilis continuously flips these genes on and off. When faced with more stress, it increases the frequency of these pulses. The pulsating action is like switching your heater on full blast for a brief period every few minutes, and turning it on and off more frequently if you want the house to be warmer.
"It's a very different view of how a cell can respond to a particular stress," says James Locke, a postdoctoral scholar at Caltech. Locke and graduate student Jonathan Young are the lead authors on a paper describing this work, which was published in the October 21 issue of Science.
To make their finding, the researchers introduced a chemical to B. subtilis that inhibits the production of ATP, the energy-carrying molecules of cells. The team found that the stress induced by this chemical triggers interactions within a set of genes—collectively called a genetic circuit. This circuit, which contains a set of positive and negative feedback loops, generates sustained pulses of activity in a key regulatory protein called σB ("sigma B"). The researchers attached fluorescent proteins to the circuit, causing the cells to glow green when σB was activated. By making movies of the flashing cells, the team could then study the dynamics of the circuit.
The key to this pulsating mechanism is the variability inherent in how proteins are made, the researchers say. The number of copies of any specific protein in a given cell fluctuates over time. The bacterial gene circuit amplifies these molecular fluctuations, also called noise, to generate discrete pulses of σB activation. The stress also activates another key protein that modulates the pulse frequencies.
By turning a steady input (the stress) into an oscillating output (the activation of σB) the genetic circuit is analogous to an electrical inverter, a device that converts direct current (DC) into alternating current (AC), explains Michael Elowitz, professor of biology and bioengineering at Caltech, Howard Hughes Medical Institute investigator, and coauthor of the paper. "You might think you need some kind of elaborate circuitry to implement that, but the cell can do it with just a few proteins, and by taking advantage of noise."
This work provides a blueprint for how relatively simple genetic circuits can generate complex and dynamic behaviors in individual cells, the researchers say. "We're excited to think that similar mechanisms may occur in other cellular processes," Locke says. "It'd be interesting in the future to see which aspects of this circuit architecture also appear in more complex systems, such as mammalian cells."
"With this work and recent work in other systems, we're starting to get a glimpse of just how dynamic cellular control systems really are," Elowitz adds. "That's something that was very difficult to see in the past."
The other authors of the Science paper, "Stochastic pulse regulation in bacterial stress response," are research technicians Michelle Fontes and Maria Jesus Hernandez Jimenez. The research was funded by the National Institutes of Health, the National Science Foundation, the Packard Foundation, the International Human Frontier Science Organization, and the European Molecular Biology Organization.
PASADENA, Calif.—Many meat-eating animals have unique ways
of hunting down a meal using their senses. To find a tasty treat,
bats use echolocation, snakes rely on infrared vision, and owls
take advantage of the concave feathers on their faces, the better
to help them hear possible prey. Leeches have not just one but two
distinct ways of detecting dinner, and, according to new findings
from biologists at the California Institute of Technology
(Caltech), their preferred method changes as they age.
Medicinal leeches, like many aquatic animals, use water
disturbances to help them find a meal. Juvenile leeches eat the
blood of fish and amphibians, while adults opt for blood meals from
the more nutritious mammals. Since it was known that leeches change
their food sources as they develop, the Caltech team wanted to know
if the way they sense potential food changed as well. Their
findings are outlined in a paper now available online in
the Journal of Experimental Biology.
The group set up experiments to test how much leeches rely on
each of the two sensory modalities they use to find food: hairs on
their bodies that can note disturbances in the water made by prey
moving through it and simple eyes that can pick up on the passing
shadows that those waves make. They monitored both juvenile and
adult leeches as they reacted to mechanical waves in a tank of
water or to passing shadows, as well as to a combination of the two
stimuli. The leeches in both age groups responded in similar ways
when only one stimulus was present. But when both waves and shadows
existed, the adult leeches responded solely to the waves.
"We knew that there was a developmental switch in what kind of
prey they go after," says Daniel Wagenaar, senior author of the
paper and Broad Senior Research Fellow in Brain Circuitry at
Caltech. "So when we saw a difference in the source of disturbances
that the juveniles go after relative to the adults, we thought
'great—it's probably matching what we know.'"
However, the team was very surprised to see that the individual
sensory modalities aren't modified during development to help
decipher different types of prey. The leech's visual system doesn't
really change as the animal matures; neither does the mechanical
system. What does change, however, is the integration of the visual
and mechanical cues to make a final behavioral decision.
"As they mature, the animals basically start paying attention to
one sense more than the other," explains Cynthia Harley, lead
author of the study and a postdoctoral scholar in biology at
Caltech. She says that the team will now focus their studies on the
adult leeches to learn more about how this sensory information is
processed both at the behavioral and cellular levels.
Paper coauthor Javier Cienfuegos, now a freshman at Yale,
contributed to the study while a high school student at the
Polytechnic School, which is located next to Caltech's campus. He
ran about half of the experimental trials and was "instrumental in
the success of the study," says Harley.
The research outlined in the paper, "Developmentally regulated
multisensory integration for prey localization in the medicinal
leech," was funded by the Burroughs Wellcome
Fund and the Broad Foundations.
PASADENA, Calif.—Using highly potent antibodies isolated from HIV-positive people, researchers have recently begun to identify ways to broadly neutralize the many possible subtypes of HIV. Now, a team led by biologists at the California Institute of Technology (Caltech) has built upon one of these naturally occurring antibodies to create a stronger version they believe is a better candidate for clinical applications.
Current advances in isolating antibodies from HIV-infected individuals have allowed for the discovery of a large number of new, broadly neutralizing anti-HIV antibodies directed against the host receptor (CD4) binding site—a functional site on the surface of the virus that allows for cell entry and infection. Using a technique known as structure-based rational design, the team modified one already-known and particularly potent antibody—NIH45-46—so that it can target the binding site in a different and more powerful way. A study outlining their process was published in the October 27 issue of Science Express.
"NIH45-46 was already one of the most broad and potent of the known anti-HIV antibodies," says Pamela Bjorkman, Max Delbrück Professor of Biology at Caltech and senior author on the study. "Our new antibody is now arguably the best of the currently available, broadly neutralizing anti-HIV antibodies."
By conducting structural studies, the researchers were able to identify how NIH45-46 interacted with gp120—a protein on the surface of the virus that's required for the successful entry of HIV into cells—to neutralize the virus. Using this information, they were able to create a new antibody (dubbed NIH45-46G54W) that is better able to grab onto and interfere with gp120. This improves the antibody's breadth—or extent to which it effectively targets many subtypes of HIV—and potency by an order of magnitude, according to Ron Diskin, a postdoctoral scholar in Bjorkman's lab at Caltech and the paper's lead author.
"Not only did we design an improved version of NIH45-46, our structural data are calling into question previous assumptions about how to make a vaccine in order to elicit such antibodies," says Diskin. "We hope that these observations will help to guide and improve future immunogen design."
By improving the efficacy of antibodies that can neutralize HIV, the researchers point to the possibility of clinical testing for NIH45-46G54W and other antibodies as therapeutic agents. It's also plausible that understanding effective neutralization by powerful antibodies may be useful in vaccine development.
"The results uncover the structural underpinnings of anti-HIV antibody breadth and potency, offer a new view of neutralization by CD4-binding site anti-HIV antibodies, and establish principles that may enable the creation of a new group of HIV therapeutics," says Bjorkman, who is also a Howard Hughes Medical Institute investigator.
Other Caltech authors on the study, "Increasing the Potency and Breadth of an HIV Antibody by Using Structure-Based Rational Design," include Paola M. Marcovecchio, Anthony P. West, Jr., Han Gao, and Priyanthi N.P. Gnanapragasm. Johannes Scheid, Florian Klein, Alexander Abadir, and Michel Nussenweig from Rockefeller University, and Michael Seaman from Beth Israel Deaconess Medical Center in Boston also contributed to the paper. The research was funded by the Bill & Melinda Gates Foundation, the National Institutes of Health, the Gordon and Betty Moore Foundation, and the German Research Foundation.
PASADENA, Calif.—Mark E. Davis and David A. Tirrell of the California Institute of Technology (Caltech) have been elected to the Institute of Medicine (IOM), an honor that is considered among the highest in the fields of health and medicine. Both Davis and Tirrell are already members of the National Academy of Sciences and the National Academy of Engineering, making them two of only 13 living individuals who have been elected to all three branches of the National Academies.
"Both Mark and Dave have made important interdisciplinary contributions that span the fields of chemical and biomolecular engineering," says Jacqueline Barton, the Arthur and Marian Hanisch Memorial Professor of Chemistry and chair of the Division of Chemistry and Chemical Engineering at Caltech. "It is fitting that they be honored by all three academies."
Although election to all three branches of the academies is a rare distinction, Davis and Tirrell are not the first from Caltech to earn the honor—the Institute now has three faculty members and two alumni on the list of 13. Frances Arnold, the Dick and Barbara Dickinson Professor of Chemical Engineering, Bioengineering, and Biochemistry joined the ranks in 2008, and alumni Leroy Hood and Yuan-Cheng Fung are also on the list.
Mark Davis, the Warren and Katharine Schlinger Professor of Chemical Engineering, is a member of the experimental therapeutics program at the City of Hope Comprehensive Cancer Center. His research focuses on the design and synthesis of nanoscale materials that are designed to be combined with therapeutic molecules. These "nanomedicines" have the potential to change the way cancer is treated—by providing more targeted therapies with fewer side effects—and are currently being tested in clinical trials.
"I am honored to receive this recognition," Davis says, "as it gives us validation that the medical community appreciates our work on creating new cancer therapeutics."
Davis earned his BS, MS, and PhD at the University of Kentucky in 1977, 1978, and 1981, respectively. He joined the Caltech faculty as a professor in 1991, was named Schlinger Professor in 1993, and served as executive officer for chemical engineering from 1999 to 2004.
David Tirrell, the Ross McCollum-William H. Corcoran Professor and professor of chemistry and chemical engineering, is known for work that bridges chemistry, biology, and materials science. Tirrell has developed a method for getting bacterial cells to "read" artificial genes and then produce protein-like structures with unusual or desired properties. The new materials could be useful in biomedical applications.
"It's always nice when a group of colleagues indicates that they think the research going on in your laboratory is worthwhile," Tirrell says. "My students and postdocs work on fundamental problems in protein chemistry, usually without specific clinical objectives. But we hope that what we do might someday find its way into medical practice and into other areas of science and technology."
Tirrell received his BS from MIT in 1974 and his PhD from the University of Massachusetts in 1978. He joined Caltech's faculty in 1998 and served as chair of the Division of Chemistry and Chemical Engineering from 1999 until 2009.
The IOM was established in 1970 by the National Academy of Sciences and is recognized as "a national resource for independent, scientifically informed analysis and recommendations on human health issues."
The election of Davis and Tirrell brings Caltech's total representation in the IOM to six faculty members and two trustees. This year, 65 new members and five foreign associates were elected to the IOM, bringing the total active membership to 1,688 members and the total number of foreign associates to 102.
PASADENA, Calif.—The California Institute of Technology (Caltech) has been rated the world's number one university in the 2011–2012 Times Higher Education global ranking of the top 200 universities, displacing Harvard University from the top spot for the first time in the survey's eight-year history.
Caltech was number two in the 2010–2011 ranking; Harvard and Stanford University share the second spot in the 2011–2012 survey, while the University of Oxford and Princeton University round out the top five.
"It's gratifying to be recognized for the work we do here and the impact it has—both on our students and on the global community," says Caltech president Jean-Lou Chameau. "Today's announcement reinforces Caltech's legacy of innovation, and our unwavering dedication to giving our extraordinary people the environment and resources with which to pursue their best ideas. It's also truly gratifying to see three California schools—including my alma mater, Stanford—in the top ten."
Thirteen performance indicators representing research (worth 30% of a school's overall ranking score), teaching (30%), citations (30%), international outlook (which includes the total numbers of international students and faculty and the ratio of scholarly papers with international collaborators; 7.5%), and industry income (a measure of innovation; 2.5%) are included in the data. Among the measures included are a reputation survey of 17,500 academics; institutional, industry, and faculty research income; and an analysis of 50 million scholarly papers to determine the average number of citations per scholarly paper, a measure of research impact.
"We know that innovation is the driver of the global economy, and is especially important during times of economic volatility," says Kent Kresa, chairman of the Caltech Board of Trustees. "I am pleased that Caltech is being recognized for its leadership and impact; this just confirms what many of us have known for a long time about this extraordinary place."
"Caltech has been one of California's best-kept secrets for a long time," says Caltech trustee Narendra Gupta. "But I think the secret is out!"
Times Higher Education, which compiled the listing using data supplied by Thomson Reuters, reports that this year's methodology was refined to ensure that universities with particular strength in the arts, humanities, and social sciences are placed on a more equal footing with those with a specialty in science subjects. Caltech—described in a Times Higher Education press release as "much younger, smaller, and specialised" than Harvard—was nevertheless ranked the highest based on their metrics.
According to Phil Baty, editor of the Times Higher Education World University Rankings, "the differences at the top of the university rankings are miniscule, but Caltech just pips Harvard with marginally better scores for 'research—volume, income, and reputation,' research influence, and the income it attracts from industry. With differentials so slight, a simple factor plays a decisive role in determining rank order: money."
"Harvard reported funding increases similar in proportion to other institutions, whereas Caltech reported a steep rise (16%) in research funding and an increase in total institutional income," Baty says.
Data for the Times Higher Education's World University Rankings was provided by Thomson Reuters from its Global Institutional Profiles Project (http://science.thomsonreuters.com/globalprofilesproject/), an ongoing, multistage process to collect and validate factual data about academic institutional performance across a variety of aspects and multiple disciplines.
For a full list of the world's top 200 schools and all of the performance indicators, go to http://www.timeshighereducation.co.uk/world-university-rankings/.
# # #
The California Institute of Technology (Caltech) is a small, private university in Pasadena that conducts instruction and research in science and engineering, with a student body of about 900 undergraduates and 1,200 graduate students. Recognized for its outstanding faculty, including several Nobel laureates, and such renowned off-campus facilities as the Jet Propulsion Laboratory, the W. M. Keck Observatory, and the Palomar Observatory, Caltech is one of the world's preeminent research centers.
PASADENA, Calif.—The cameras in our cell phones have
dramatically changed the way we share the special moments in our
lives, making photographs instantly available to friends and
family. Now, the imaging sensor chips that form the heart of these
built-in cameras are helping engineers at the California Institute
of Technology (Caltech) transform the way cell cultures are imaged
by serving as the platform for a "smart" petri dish.
Dubbed ePetri, the device is described in a paper that appears
online this week in the Proceedings of the National Academy of
Sciences (PNAS).
Since the late 1800s, biologists have used petri dishes
primarily to grow cells. In the medical field, they are used to
identify bacterial infections, such as tuberculosis. Conventional
use of a petri dish requires that the cells being cultured be
placed in an incubator to grow. As the sample grows, it is
removed—often numerous times—from the incubator to be
studied under a microscope.
Not so with the ePetri, whose platform does away with the need
for bulky microscopes and significantly reduces human labor time,
while improving the way in which the culture growth can be
"Our ePetri dish is a compact, small, lens-free microscopy
imaging platform. We can directly track the cell culture or
bacteria culture within the incubator," explains Guoan Zheng,
lead author of the study and a graduate student in electrical
engineering at Caltech. "The data from the ePetri dish
automatically transfers to a computer outside the incubator by
a cable connection. Therefore, this technology can significantly
streamline and improve cell culture experiments by cutting down on
human labor and contamination risks."
The team built the platform prototype using a Google smart
phone, a commercially available cell-phone image sensor,
and Lego building blocks. The culture is placed on the image-sensor
chip, while the phone's LED screen is used as a scanning light
source. The device is placed in an incubator with a wire running
from the chip to a laptop outside the incubator. As the image
sensor takes pictures of the culture, that information is sent out
to the laptop, enabling the researchers to acquire and save images
of the cells as they are growing in real time. The technology is
particularly adept at imaging confluent cells—those that grow
very close to one another and typically cover the entire petri
"Until now, imaging of confluent cell cultures has been a highly
labor-intensive process in which the traditional microscope has to
serve as an expensive and suboptimal workhorse," says Changhuei
Yang, senior author of the study and professor of electrical
engineering and bioengineering at Caltech. "What this technology
allows us to do is create a system in which you can do wide
field-of-view microscopy imaging of confluent cell samples. It
capitalizes on the use of readily available image-sensor
technology, which is found in all cell-phone cameras."
In addition to simplifying medical diagnostic tests, the ePetri
platform may be useful in various other areas, such as drug
screening and the detection of toxic compounds. It has also
proved to be practical for use in basic research.
Caltech biologist Michael Elowitz, a coauthor on the study, has
put the ePetri system to the test, using it to observe embryonic
stem cells. Stem cells in different parts of a petri dish often
behave differently, changing into various types of other, more
specialized cells. Using a conventional microscope with its lens's
limitations, a researcher effectively wears blinders and is only
able to focus on one region of the petri dish at a time, says
Elowitz. But by using the ePetri platform, Elowitz was able to
follow the stem-cell changes over the entire surface of the
"It radically reconceives the whole idea of what a light
microscope is," says Elowitz, a professor of biology and
bioengineering at Caltech and a Howard Hughes Medical Institute
investigator. "Instead of a large, heavy instrument full of
delicate lenses, Yang and his team have invented a compact
lightweight microscope with no lens at all, yet one that can still
produce high-resolution images of living cells. Not only that, it
can do so dynamically, following events over time in live cells,
and across a wide range of spatial scales from the subcellular to
the macroscopic."
Elowitz says the technology can capture things that would
otherwise be difficult or impossible—even with
state-of-the-art light microscopes that are both much more
complicated and much more expensive.
"With ePetri, you can survey the entire field at once, but still
maintain the ability to 'zoom in' to any cells of interest," he
says. "In this regard, perhaps it's a bit like an episode of CSI
where they zoom in on what would otherwise be unresolvable details
in a photograph."
Yang and his team believe the ePetri system is likely to open up
a whole range of new approaches to many other biological systems as
well. Since it is a platform technology, it can be applied to other
devices. For example, ePetri could provide microscopy-imaging
capabilities for other portable diagnostic lab-on-a-chip tools. The
team is also working to build a self-contained system that would
include its own small incubator. This advance would make the system
more useful as a desktop diagnostic tool that could be housed in a
doctor's office, reducing the need to send bacteria samples out to
a lab for testing.
Seung Ah Lee, a graduate student in electrical engineering, and
Yaron Antebi, a postdoctoral scholar in biology—both from
Caltech—were also coauthors on the study, which is titled
"The ePetri dish, an on-chip cell imaging platform based on
subpixel perspective sweeping microscopy (SPSM)." Funding support
was provided by the Coulter Foundation.
Written by Katie Neith
PASADENA, Calif.—Responding to faces is a critical tool
for social interactions between humans. Without the ability to read
faces and their expressions, it would be hard to tell friends from
strangers upon first glance, let alone a sad person from a happy
one. Now, neuroscientists from the California Institute of
Technology (Caltech), with the help of collaborators at Huntington
Memorial Hospital in Pasadena and Cedars-Sinai Medical Center in
Los Angeles, have discovered a novel response to human faces by
looking at recordings from brain cells in neurosurgical
The finding, described in the journal Current Biology,
provides the first description of neurons that respond strongly
when the patient sees an entire face, but respond much less to a
face in which only a very small region has been erased.
"The finding really surprised us," says Ueli Rutishauser, first
author on the paper, a former postdoctoral fellow at Caltech, and
now a visitor in the Division of Biology. "Here you have neurons
that respond well to seeing pictures of whole faces, but when you
show them only parts of faces, they actually respond less and less
the more of the face you show. That just seems
The neurons are located in a brain region called the amygdala,
which has long been known to be important for the processing of
emotions. However, the study results strengthen a growing belief
among researchers that the amygdala has also a more general role in
the processing of, and learning about, social stimuli such as
Other researchers have described the amygdala's neuronal
response to faces before, but this dramatic selectivity—which
requires the face to be whole in order to elicit a
response—is a new insight.
"Our interpretation of this initially puzzling effect is that
the brain cares about representing the entire face, and needs to be
highly sensitive to anything wrong with the face, like a part
missing," explains Ralph Adolphs, senior author on the study and
Bren Professor of Psychology and Neuroscience and professor of
biology at Caltech. "This is probably an important mechanism to
ensure that we do not mistake one person for another and to help us
keep track of many individuals."
The team recorded brain-cell responses in human participants who
were awaiting surgery for drug-resistant epileptic seizures. As
part of the preparation for surgery, the patients had electrodes
implanted in their medial temporal lobes, the area of the brain
where the amygdala is located. By using special clinical electrodes
that have very small wires inserted, the researchers were able to
observe the firings of individual neurons as participants looked at
images of whole faces and partially revealed faces. The
voluntary participants provided the researchers with a unique and
very rare opportunity to measure responses from single neurons
through the implanted depth electrodes, says Rutishauser.
"This is really a dream collaboration for basic research
scientists," he says. "At Caltech, we are very fortunate to have
several nearby hospitals at which the neurosurgeons are interested
in such collaborative medical research."
The team plans to continue their studies by looking at how the
same neurons respond to emotional stimuli. This future work,
combined with the present study results, could be highly valuable
for understanding a variety of psychiatric diseases in which this
region of the brain is thought to function abnormally, such as mood
disorders and autism.
Other Caltech authors on the paper are Oana Tudusciuc, a
postdoctoral scholar in neuroscience and psychology, and Dirk
Neumann, visiting associate in biology. Medical collaborators on
the study include Adam Mamelak, a neurosurgeon at Cedars-Sinai; and
Huntington Memorial Hospital's Christopher Heller, neurosurgeon;
Ian Ross, neurosurgeon; Linda Philpott, neuropsychologist; and
William Sutherling, medical director of the Epilepsy and Brain
Mapping Program. The work in the paper "Neuronal responses
selective for whole faces in the human amygdala," was supported by
the National Science Foundation, the Pfeiffer Family Foundation,
and the Gordon and Betty Moore Foundation.


text:
Proper Winch Pulling Power ???
I was told that the winch on your vehicle had to have the power to pull 1.5 times the weight of your vehicle, because if your Jeep is stuck, that winch is pulling, not only the weight of the Jeep, but also has to overcome the mud that has the Jeep stuck.
Is 1.5 times the weight a good number to go by? Or, do you need more pulling power than this?
From what I've seen it's "at least" 1.5 times.. Everything I've seen says you need at minimum a 8k winch..
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Planning For Vacation
As soon as we hear the words "Summer Vacation", images of carefree fun stir our mind. But to make our vacations perfect and memorable, proper planning is required.
- Posted 6 years ago


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Good Hunting: Prey Client for Linux
Following the release of the Prey Demo, a Linux Client for the game is now available.
Game fans can download the Prey Linux Installer from the website Icculus.org. In Prey, the Indian Tommy and his family are abducted by aliens orbiting the earth in a spaceship. Tommy battles the aliens, his physical and mental capabilities becoming more powerful as the game progresses. His Indian ancestry gives him a couple of special possibilities; he can leave his body to scout the environment ahead. Interesting is that gravity on board the spaceship can be manipulated, allowing players to walk on ceilings and walls.
Along with a 2GHz processor, 512MByte RAM and a 64MByte graphic card, the 3 CD original PC version or the DVD Collectors Edition are necessary to run the game. The installer will also recognize the Steam download. In any case, players must be in possession of a unique and valid CD key.
The reasonably sized 43MByte BIN file makes the installer easily manageable. To install Prey, users must direct the installer to the mounted DVD or the NTFS disc:
./prey-installer-12072008.bin --from-install --media '/mnt/ntfsdisk/Program Files/Steam/steamapps/common/prey/base'
Issue 187/2016
Buy this issue as a PDF
Linux is the Largest Software Development Project in History
But if you are not using the latest Linux kernel, your system is insecure.
Linksys Says Yes to Open Source Firmware
Home routers will give room for custom firmware but still comply with FCC rules
OwnCloud Founder Resigns
Frank Karlitschek will continue to lead the open source ownCloud project
Ubuntu 16.04 LTS released
"Xenial Xerus" comes with a new packages format and several improvements for the enterprise.
MS Visual Studio Code Editor is Available for Linux
Linux users can now download and install the Windows code editor
Open Container Initiative announces Image Format Project
New initiative will address security and interoperability concerns around container technology.
Red Hat Lures Developers with Free RHEL and Container Development Kits
Developers can use RHEL as a development platform without a subscription fee.
Microsoft Brings the Linux Command Line to Windows
Windows users will soon have native access to the Bash shell.
Internet Research Group Proposes Better Email Standard
Improvements to SMTP will provide better guarantee of confidentiality
New Nvidia Driver Offers Wayland Support
Graphics vendor embraces new reality in Linux graphics


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Contact Information
277 Main St Belleville, NJ 07109
277 Main StreetUSNew JerseyBelleville07109
(973) 751-9400
Pet Supply Store
Honey Bee Pets islocated in Belleville, New Jersey. No matter what type of pet you have, you should always provide the best food and supplies. You don't have to spend a fortune to ensure the health and happiness of your pet. Come to Honey Bee Pets for great prices on all the brands that you're looking for. You can have the quality food and supplies you need for your pet delivered right to your door. Not only will you find the best products for your pet, but you will also receive superior service from our team of professionals who strive to exceed your expectations.
Google+ URL


text:
Question about HP Deskjet F4180 All-in-One InkJet Printer
Posted by jeje262 on
Hi Jeje,
The Printer has a fatal error. Please try the below steps, if it didn't work you have to service the printer.
Power Drain:
NVM Reset:
Posted on Jan 19, 2010
Tips for a great answer:
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text:
Liquor prices in Washington fell gradually through the summer, taking the edge off higher prices that surprised some drinkers — and voters — when private retailers began selling spirits June 1.
Year-over-year spirits sales increased in July and August after falling in June, according to the Washington State Department of Revenue.
Some thought private liquor sales would mean lower prices, but Washington's high liquor taxes remain in place, and the initiative voters passed last fall to privatize spirits sales includes fees assessed on retailers and distributors that help keep prices high. The intent of the fees was to keep money flowing to the state and local governments, which in fiscal 2011 received $416 million from the state-run liquor business.
The result on grocery shelves was an average 14.8 percent price hike for liquor sold in June, compared with June 2011. By July, the average price increase slowed to 12.4 percent. And in August, prices were 10.1 percent higher than August 2011.
- Seattle's vanishing black community
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The average price of a liter of liquor in August 2012 was $24.73, compared with $22.46 the previous August. Both prices are after taxes.
Prices could fall further because of competition and, in two years, a decrease in the fee paid by distributors from 10 percent to 5 percent of liquor sales.
Liquor sales, which fell 9.4 percent in June, the first month of privatization, have rebounded. They rose 15.4 percent in July and 11.8 percent in August.
That includes sales in restaurants, bars and retail stores. Sales in restaurants and bars alone fell in June and remain below last year's levels.
Melissa Allison: 206-464-3312 or email@example.com. Twitter @AllisonSeattle.


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SPICE (Image 1)
A Science Partners for Inquiry-based Collaborative Education (SPICE) participant nets for macroinvertebrates.
Now in its second track of funding by the National Science Foundation (grant DGE 05-38407), SPICE was created to train University of Florida (UF) graduate students in teaching and to foster inquiry-based learning of science, technology, engineering and mathematics in Gainesville's under-resourced middle schools.
SPICE is a collaboration of individuals from different departments, colleges, centers and schools at UF, who are teamed with six middle schools and the public schools of Alachua County. Graduate students from UF work in the classroom with middle school teachers to guide students on an exploratory quest about ecosystem health. (Date of Image: 2003-2005) [One of 3 related images. See Next Image.]


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Friday, March 21, 2008
Light of the World
My oldest son came down with one of those icky stomach bugs, so we had to postpone our trip to Baltimore for a few weeks. The good news is the time I spent on extra laundry and steam cleaning carpets was rewarded with a little bit of unexpected stamping time last night and today.
The first stamp release from Our Daily Bread Designs, called Light of the World, came yesterday. This card didn't turn out exactly like the vision in my head (which was a little too big for my stamping ability!), but I do still like it. I had not done one of the shadow box cards before. I also tried a new-to-me technique, which is the salted background. SCS has some great tutorials on both. The links will take you right to those tutorials. For the salted background, I painted watercolor paper with Night of Navy and Bordering Blue reinkers, sprinkled with table salt, spritzed with water, and then dried it with a heat tool. I brushed off the dried up salt before stamping the Bible verse in Jet Black Staz-On. I drew inspiration for my card from this card that Laurie Schmidlin made using the same stamp set.
Tip: I cut my ovals with the Creative Memories cutting system. This cutting system (and most, it seems) only cuts mats in 1/8" increments. I much prefer the narrower 1/16" increment. I read this little tip on someone's blog several months ago (I wish I could remember whose so I could give credit). In this case, where you're cutting out a hole, cut your two inside layers the same size (if you're not cutting out a frame, but matting an image instead, you'll cut your two outside layers the same size). For the middle layer (white here) use scissors to trim away just a tiny amount. Of course, it will be uneven when it is hand-cut, so take the edge distresser and rough it up a bit. Now it's supposed to be messy, so the unevenness isn't a problem.
So, this is my first-ever offical design team creation. Isn't that fun?
Now, last but not least, would you like to win this stamp set? Check out the blog candy celebration, which is in the post right below!
Stamps: Light of the World (by Our Daily Bread Designs)
Paper: watercolor paper, Night of Navy, Bordering Blue, Whisper White
Ink: Night of Navy, Bordering Blue, Creamy Caramel, Close to Cocoa, So Saffron, Always Artichoke, Jet Black Staz-On
Accessories: aqua painter, table salt, water spritzer, paper piercer, sponge daubers, distressing tool, silver brads, corner rounder punch, pretties kit, three for you punch, 1/4" punch
14 comments:
This is such a great card again! :) I really love what you are making with this stamp set!ReplyDelete
- AnonymousMarch 21, 2008 at 7:31 PM
Fantastic card. Thanks for offering that tip about using the Creative Memories cutting system. I've got it, too, and am always wishing for the 1/16" edge.ReplyDelete
Happy Easter - Regina Davis
wowzers!!! you have set your bar very high as this being your forst design team submission...it is beautiful! great job!ReplyDelete
LOVE your card and am so glad we are on this design team together!ReplyDelete
WOW!!! At least I can say, "I knew you when..."ReplyDelete
Just beautiful Amy, your watercoloring is gorgeous along with the fun design. Have a great weekend, Jennifer :)ReplyDelete
Amy, this is breathtaking! Wonderful design! Thank you so muc for the tip on cutting and matting. I love that look!ReplyDelete
Stunning! I love the scallops along the top! Such a pretty card!ReplyDelete
Amy you did an awesome job on this!!!ReplyDelete
Absolutely fabulous! I love the design on the sides. The showdow box looks fabulous, as does the salted background. Beautiful colors too!!ReplyDelete
This is so beautiful!! I love the design..and your coloring is gorgeous!!!ReplyDelete
- Mary DavidsonMarch 23, 2008 at 11:01 AM
Beautiful card, Amy! Thanks for the tip on cutting shapes for matting! Love the set!ReplyDelete
Simply GORGEOUSReplyDelete
Oh, I just love shadowbox cards. all those brads on the sides give a fabulous feel to the card. Sorry I'm commenting so much all in one day, but this is the first time I've had in about a week to "blurf". Love everything I've seen! :)ReplyDelete


text: I have recently installed GeForce GT 430 video card and it has been installed without ant problem but then the problem that I am facing now is that the computer reboots automatically after 15 minutes into a blu ray. I am also having a HTPC has a 270watt PSU and I think that there is something wrong with it but then I am not sure about the same. I also wanted to know that if I want to keep it under powered than what will be the best PSU?? Any help regarding the same will be appreciated.


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ARO, Inc. provides leading-edge business process outsourcing (BPO) for insurance companies. Five years ago, our first step into the insurance field included theMore
$5 mil. - $10 mil.in Revenue
100 - 250EmployeesContact people at this company
missouribased call-centerfinancial services outsourcingMore
Call Centers & Business CentersBusiness ServicesMore
3100 Broadway Suite 100Kansas CityMissouri64111United Statesview map
(866) 587-8555
ProPub_Iteration_NT.411[04] SEIDXWK-23


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Olga's store is located on the community's main street and is the only grocery business in the community. She attends to many customers and plans to use the loan to improve her business so she can increase her income and live a better lifestyle.
Olga will use the loan to buy products such as rice, sugar, beans, lard, sausages, milk, cheese, butter, margarine, chicken, beef, pork, etc. Her goal is to expand her business and prosper together with her family, while providing a good education for her son.
María's business for the last 26 years has been selling clothing, cosmetics, shoes, catalogue products, fish and crab.
Danlí, Honduras - December 2012
Doña Olga tiene ubicado su negocio en la calle principal de su comunidad siendo este la única pulpería y atiende una gran cantidad de personas, el préstamo que reciba lo ocupa para mejorar su negocio y a su vez mejorar sus ingresos y cambiar un poco su condición de vida.
Doña Olga solicita el préstamo para la compra de producto como ser: arroz, azúcar, frijoles, manteca, embutidos, leches, quesos, mantequilla, margarina, carnes de pollo, res, cerdos, etc. sueña con que su negocio se mas grande y poder salir adelante con su familia dandole una buena educación a su hijo.
Doña María su fuente principal de ingresos proviene de su negocio venta ropa, cosméticos, zapatos productos por catalogo, pescado y jaiba, desde hace 26 años.
Danlí, Honduras - Diciembre 2012
Additional Information
Prisma Honduras, S.A.This loan is administered by Prisma Honduras. Prisma is one of Kiva's first field partners in Central America and has funded over 2,500 entrepreneurs through Kiva since 2008. Prisma funds smaller than average loans for micro-entrepreneurs to help them grow businesses in urban and rural areas. To improve living conditions in Honduras, the organization strives to empower women who lack access to traditional financial services, and promotes solar panels to deliver clean, affordable electricity to rural areas. Through its four branches in southern and central Honduras, Prisma provides access to financial products to some of the poorest and most isolated communities in the country.
This is a Group Loan
In a group loan, each member of the group receives an individual loan but is part of a larger group of individuals. The group is there to provide support to the members and to provide a system of peer pressure, but groups may or may not be formally bound by a group guarantee. In cases where there is a group guarantee, members of the group are responsible for paying back the loans of their fellow group members in the case of delinquency or default.
Kiva's Field Partners typically feature one borrower from a group. The loan description, sector, and other attributes for a group loan profile are determined by the featured borrower's loan. The other members of the group are not required to use their loans for the same purpose.
About Honduras
35View loans »
Success!! The loan was 100% repaid
- Repayment Term
- 10 months (Additional Information)
- Repayment Schedule
- Monthly
- Pre-Disbursed:
- Dec 15, 2012
- Listed
- Feb 1, 2013
- Currency Exchange Loss:
- Possible
- Ended:
- Jun 18, 2013


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There are a lot of people who are going to lose a lot of money in the next few months. First off ZLCS tried to run up over $1 twice now in the past month and was not able to make it. With every day that passes it is one day clower to being de-listed from the Nasdaq for not meeing the $1 minimum. Once big Z gets to be 7 days out from de-listing with their stock price hovering in the 50's they will enact a reverse split for 8:1. After the split shares will drop another 20% by the beginning of November trading at around $3.40 / .42 cents pre split.
This is inevitable as there will be no news released between now and then. They had two news events in the last five days and the stock has gone down 14% as a result. It will be a slow 3% a day trickle with a few cliff drops in between now and early October.
Now to the failure of Z160. They have promised to report results in Q4. That is scam biotech speak for Friday afternoon December 27th. Look for them to report that while there were no significant safety issues, the expected endpoints were not met.
There will have been a small increase in share price in the month of December as some noobs catch wind of a stock with an end of the year catalysts so some buying will get this up to around $4.90 a share / 61 cents pre- split. After the fail news is announced you can expect Z's price to collapse to around $1.20 a share / 15 cents pre-split.
You will see that price drop even further as those who bought just a week before expecting good news run for the exits to take a tax loss for the year.
The best case scenario out of this is that after trading back below $1 they will come forward with news of some data mined sub groups that show positive results. That may get you a 20% increase in share price... good if you get in at those January 2014 levels... bad if you are invested as of September 2013.
not selling
My apologies, I called you an idiot because I thought you referring to "Global NASDAQ" which we have already been delisted. You're right if we don't comply we can be delisted all together from NASDAQ which is why by October 14th you'll see a R/S in an attempt to comply for ten consecutive days. The R/S was already approved by the board and voters so it's a done deal - it will happen. My guess we'll have a 6/1 split and open around a strike price of $4.50. It all depends on the pps at the time of the split.
Sentiment: Strong Buy
Never apologize to trolls.
we are not losing our money lol.going up
You are truly an idiot -- ZLCS is already delisted. ZLCS is trading under "NASDAQ Capital" and was delisted from "NASDAQ Global" back in April.....Now I know you knuckle heads are chumps.
Sentiment: Buy
Man, I remember when ACAD did something similar and promised to release results in Q4 last year. Total scam company screwed everybody. The stock was trading in the low $2 range, and now it's trading over $20. What d-bags! These scam artists area all the same.
You are so smart, jill. Are you single? I'm definitely interested.
Why you are loosing so much time for the poor masses?
Ok, Clown, now let's speak about the realist one:
"HOW TO PLAY THE POSITIVE preliminary DATA RESULTS OF 2 TRIAL OF PH.2 Z160 ON OCTOBER/EARLY NOVEMBER"
and then you can concentrate on :
Sentiment: Strong Buy
You know what's funny???
Thx idiots like him we're getting cheaper shares and possibility to make more by trading them!
Isn't it funny?
Yep, it is!
Sentiment: Strong Buy
if you want me to get you that vibrator,you better pray that i make money out of this stock
Sentiment: Strong Buy
seeking alpha article coming soon that will burn your a.s.s....wait n see..
Sentiment: Strong Buy
Just like NVAX there BOZO? LMAO. You are working it hard just like with NVAX and you failed there. Your masters do not get their money's worth out of you. $10 a day is too much for your poor bashing. Shorter ought to hire someone else. You are very poor at your job.


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One of the most common window treatments you will find are blinds, they are incredibly versatile and are available in a wide range of styles and colours to suit a multitude of home decors.
Since 1990 we have been installing Shutters. Manufactured from suitably grown timber or aluminium all our shutters are custom-made and offer the widest range of styles, choice and finishes to suit any door or window.
A Solatube Daylighting System (DS) is a highly effective solution to daylighting any area in your home. The Solatube DS allows customers to transform dark rooms into extraordinary spaces filled with pure natural light.
Roller Shutters offer security that no other product can provide. Manufactured from aluminium, The Shutters can be used internally or externally and can be operated manually or electronically with a Somfy Motor.
Executive Blind Manufacturers was established in 1982 by Ricky Miltz. At the time we were the only manufacturer in the Eastern Cape. Now, with a staff compliment of more than 20, we have grown to manufacture Vertical Blinds, Aluminium Venetian Blinds and Wooden Venetian Blinds..
Executive Blind Manufacturers also supply Roller Blinds, Bamboo Blinds, Retro Blinds, Cellular Shade Blinds, Skylight Blinds, Sliding Panel Blinds, Roman Blinds, Vision or Zebra Blinds, Sheerweave Blinds and Motorised Blinds. We also have the more specialised products; Timber Shutters, Awnings, Roller Shutters, Solatube, Aluminium Security Shutters and Outdoor Sheerweave Blinds.
Executive Blind Manufacturers have always prided ourselves on being a family establishment that gives the best quality of product and workmanship to our clients, having insight into the latest technology within our field of expertise and making our clients happy.
365 Govan Mbeki Avenue, North End, Port Elizabeth
Tel: 041 484 7428Fax: 041 487 3106


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Puss in Boots Movie Quotes
'Puss in Boots' movie quotes are hilarious, featuring great one-liners by the film's main character, Puss in Boots (voiced by Antonio Banderas). Some quotes from the 2011 animated movie are pretty simple ("Meow" comes to mind), but others are quite clever. All have one thing in common: They are funny. Dreamworks' 'Puss in Boots' movie promises to deliver some truly enjoyable comedy animation, as viewers get to see what Puss' life was like before he made his appearance in 'Shrek.' Enjoy these 'Puss in Boots' quotes, vote for your favorites and, if you don't see your personal fave quote listed ("Holy Frijoles!"), add it!
The 3D 'Puss in Boots' movie also features the voices of Zach Galifianakis as Humpty Dumpty, Salma Hayek as Kitty Softpaws, Puss' love interest (and professional kitty-thief), Billy Bob Thornton as Jack and Amy Sedaris as Jill. In the movie, Puss joins forces with Humpty and Kitty Softpaws to try and save his town from a band of outlaws looking to destroy it. With the help of his friends, and of course, his boots, Puss can't lose!
For more family and animated movie quotes, check out these lists for the best lines from 'The Smurfs' movie, 'Cars 2,' the original 'Cars' movie and other great Pixar movies!


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Our ferries to Dover - Boulogne page is no longer available as there are currently no routes offered.
As the world's largest independent ferry comparison site with more than 1500 routes available, it is possible that we offer a suitable crossing nearby so take a look at our port, route or destinations pages for further information or contact us for advice.
You can also look for alternatives by selecting from the menus in our fare search at the top of the page.
We get live Dover to Boulogne ferry prices directly from ferry company reservation systems and compare all options ensuring you find the best deal for your crossing. Getting a price and booking your ferry ticket to France couldn't be easier!Getting a quote or booking a ferry to France couldn't be easier. All you need to do is select Dover to Boulogne from the menus to the left, select the number of passengers and hit search!


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Although my birthday was last Thursday, work and weather conspired to prevent me from the obligatory Ride Your Age birthday ride until today. Since my birthday and my sober date are the same, I rode a mile for each, and 10% for good measure. Came in at just over a metric.
Did some climbing
And some more climbing (3,803 feet of it)
Saw lots of horsies
Got faked-out by this safety decoy guy. (There were surveyors all over the place. From a distance, I thought this guy was standing in the ditch holding holding that stick thing they use.)
Was not faked-out by the plastic palm trees and giant inflatable turtle in this farm pond.
Tied the Portland to the hitchin' rack at Sweet Solutions in Ionia, NY where
I had a delicious lunch--salami and provolone wrap with mac salad and wedge of watermelon (Ooops. Ate the melon before I remembered to take a picture.)
And topped it off with a root beer float. Mmmmm. Bike fuel!


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Hello everyone!
Welcome to the next installment of Lean Startup Helsinki bar discussion series. Grab a drink, a seat and bring all your inquisitionary questions to be answered.
It was my first meetup, and I liked it. We had a lively discussion with interesting people, only challenge was that maybe the venue is getting too small for this group. Will attend again.
0 · March 22, 2012
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the sell off not coming.
What a bagholder you are!
Sentiment: Strong Sell
If you do not think a sell off is coming....you are living in LALA LAND. GL.
Sentiment: Sell
Already trading at a 48x earnings. No one will upgrade from here.
you must be referred to the 1:2 reverse stock split
earnings were yesterday-there was an upgrade today with a price target of 37- only thing that is coming monday is downgrade for over value-maybe an increase in pps early from margin calls-motley fool already wrote an article saying so


text:
An e·bill is a monthly email notice sent to any student with a balance due or any activity on their student account notifying them that their latest billing statement is now available online at USCe.pay. These emailed notices are also sent to USCe.pay and OASIS guest user email addresses.PLEASE DO NOT REPLY as these email notices are sent from a notification-only email account that cannot accept incoming email.
The monthly e·bill notice includes a link to the CURRENT month's "Monthly billing topics" page with contains links to current information about upcoming deadlines and other useful information. In addition, there is an archive link at the bottom of the page to past billing topic pages.
* Email is the primary mechanism for sending official communications to USC students, see: http://scampus.usc.edu/.
If you need a billing statement with all of your fall/spring tuition & fees BEFORE the term due dateplease be sure to register at least 40 days before the term payment deadline.Summer tuition & fees are assessed at time of registration and due when billed.
USC generates billings statements every 4 to 5 weeks, after payment deadlines, 12 times per year (see monthly billing schedule). USC does not mail paper statements to currently enrolled students, however we do send e·Bills (billing notices) each month to students and their authorized guests notifying you that a new monthly billing statement is available online (if there is an outstanding balance or activity on their account during the billing period). For students who pre-register for the Fall or Spring terms in time (see Schedule of Classes for exact dates), your tuition and fees for that term will be included on the statement generated the month before the start of classes.
Additional Information:
Settlement Deadline (date payment is due)
5pm on the Friday before the start of classes in August
Due as billed (see USCe.pay billing statement for due date)
5pm on the Friday before the start of classes in January
Student Accounts | Tuition and Fees | Deferments | Past-due Accounts
Student Financial Services www.usc.edu/sfs | Office Locations | 213-740-7471 (Outside So Cal: 800-225-1222)