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Bergamotto Marino Cologne
By Gianfranco Ferre for Men
Bergamotto Marino Cologne by Gianfranco Ferre, Introduced in 2005 by gianfranco ferre its described as a sophisticated mens fragrance . Containing notes of: musk, seaweed, egyptian jasmine, oakmoss, mahogany, and tunisian orange blossom. Works well for both formal and casual occassions. All products are original, authentic name brands. We do not sell knockoffs or imitations. ... Read More
Customers Who Bought This Item Also Bought
About Gianfranco Ferre
History and background of Gianfranco Ferre. A native of Legnamo, Italy, Gianfranco Ferre studied architecture from Politechnico Milan. After graduation, he was a freelance jewelry designer for four years, until he launched his own label in 1974. Read more about Gianfranco Ferre
More Gianfranco Ferre fragrancesfor Women: In The Mood For Love Pure , Genny , Ferre (new) , Gf Ferre , Gieffeffe , more
for Men: Ferre , Gff Cologne , Gf Ferre , Pontacio 21 , Gieffeffe , more
See all Gianfranco Ferre products
Customer Comments
I wondered what the catch was...my fragrance in a large quantity for a very inexpensive price. I received my order on time, and it was exactly what I wanted. I would order from FragranceX again.
M. Phillips (Middleville, MI)
REVIEW SNAPSHOT®by PowerReviews
- Clean & fresh (5)
- Doesn't hold (3)
Best Uses
- Daily use (4)
- Fragrance Type:
- Citrus/fruity (3), Fresh/aquatic (3)
Reviewed by 6 customers
Sort by
Displaying reviews 1-5
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- Clean & Fresh
- Draws Compliments
- Great Smell
- Great Value
Best Uses
- Daily Use
Comments about Bergamotto Marino By Gianfranco Ferre:
Very Nice. Not gonna Say too much,Others May Try it!
For This Reason.....I'm Not gonna Tell ANYONE
- Fragrance Type:
- Fresh/Aquatic
- Clean & Fresh
- Doesn't Hold
Best Uses
- Anytime
- Daily Use
Comments about Bergamotto Marino By Gianfranco Ferre:
the basenotes of this fragrance are really unique. the jasmine notes are wonderful. however, it's eau de cologne, so it doesn't hold. the citrus is notable on the top notes, but it fades to a beautiful smell.
- Fragrance Type:
- Citrus/Fruity
Comments about Bergamotto Marino Cologne:
I like this cologne
- Clean & Fresh
- Crisp and Clean
- Long-Lasting
- no complaints at all
Best Uses
- Evening Use
- Everywhere
Comments about Bergamotto Marino Cologne:
all people commented about this smooth / fresh eveninig fragance, especially me
- Fragrance Type:
- Fresh/Aquatic
- Clean & Fresh
- Great Value
- Sexy
- Doesn't Hold
Best Uses
- Daily Use
- Work
Comments about Bergamotto Marino Cologne:
I have this one on daily use. I use it in the morning and add it after work day is done. Light and fresh and that's the way I like it! Unfortunately the smell doesn't hold very well, but after all it's "only" a cologne...
- Gift:
- No
- Fragrance Type:
- Citrus/Fruity, Floral, Fresh/Aquatic
Displaying reviews 1-5
Previous | Next »


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Capital Markets
Company Overview of Palmetto Partners, Ltd.
Executive Profile
Robert M. Dussler
Chairman and Chief Executive Officer, Palmetto Partners, Ltd.
|Total Calculated Compensation
|This person is connected to 2 Board Members in 2 different organizations across 1 different industries.
See Board Relationships
Mr. Robert M. Dussler, Bob, is the Chairman and Chief Executive Officer at Palmetto Partners, Ltd. He joined the firm in 1994 and is responsible for development, execution and monitoring of investment strategy. Previously Mr. Dussler was a Principal at PCI Group. Prior to that, he was the President and Chief Operating Officer at First American Bank of Georgia. Mr. Dussler was the President at Florida National Bank and the Chairman and Chief Executive Officer at Texas ...
Corporate Headquarters
711 LouisianaPhone: 713-336-7822
Houston, Texas 77002
United States
Fax: 713-336-7828
Board Members Memberships
Chairman and Chief Executive Officer
Unknown/Other Education
University of Texas - Dallas
Unknown/Other Education
University of Houston
Other Affiliations
Annual CompensationThere is no Annual Compensation data available.
Stocks OptionsThere is no Stock Options data available.
Total CompensationThere is no Total Compensation data available.
|There is no Competitor Compensation data available.
Sponsored Financial Commentaries
Sponsored Links
Request Profile Update
Only a company representative may request an update for the company profile. Documentation will be required.
To contact Palmetto Partners, Ltd., please visit . Company data is provided by Capital IQ. Please use this form to report any data issues.
Please enter your information in the following field(s):
Your requested update has been submitted
Our data partners will research the update request and update the information on this page if necessary. Research and follow-up could take several weeks. If you have questions, you can contact them at firstname.lastname@example.com.

subset: commoncrawl


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Four-inch helix tree anchor provides extra holding power
This screw-in style anchoring device is ideal for anchoring larger trees, but can also be used to anchor tents, shelters and portable sheds. The 1/2"-diameter rod on the tree anchor has 4" blades that thread quickly into the soil by inserting a bar into the 1"-diameter eye. Up to 2,500 lbs. of holding capacity under normal soil conditions. Tree anchors are sold in packages of 12. USA made.
2016 Master Catalog: Page 418


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WCB co-sponsor for Dr. Michael Watkins as part of Joseph-Beth Business Speaker Series
The First 90 Days: Proven Strategies for Getting Up to Speed Faster and Smarter
When a new leader fails to thrive in the first few months on the job, it can be a severe, perhaps career-ending blow to the individual. Even worse — missteps in those first few months can often jeopardize and derail success for an entire organization.
At this "Must Attend" event, leadership transition expert Michael D. Watkins will address today's demanding professional landscape, where managers face not only more frequent transitions but also steeper expectations once they step into their new jobs. He will offer new tools to help anyone get up-to-speed quickly.
Michael D. Watkins is the cofounder of Genesis Advisers, a leadership development consultancy that specializes in the design of onboarding and transition acceleration solutions, workshops, and coaching. He is on the faculty of the International Institute of Management Development (IMD) in Lausanne, Switzerland, and former professor at Harvard Business School and the JFK School at Harvard.
July 18, 2013 l 5:00pm | The Madison Event Center | 700 Madison Avenue l Covington, Kentucky 41011
RXVP at http://ctomasetti.wix.com/the-first-90-days


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Bjorn Borg, who's been traveling Asia on the whirlwind Champions Tour, has just launched an online dating site "Love For All" which lives on his eponymous underwear website.
The tennis legend is promoting the new effort through a provocative viral video featuring the marriage of two priests.
Love for all? Hell yeah.
Check it:
(via Towleroad)
Wednesday, November 26, 2008
VIDEO VAULT: Even two priests can marry in Bjorn Borg's world
Labels: Bjorn Borg, tennis, video
3 comments:
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Most lodgings should be submitted to the locality where they are physically located. To find the proper locality and submit there, navigate to the list of localities found under the name of this state.Places to stay throughout the county.
Note: Each lodging facility may receive one listing. Attempts to circumvent this policy by multiple submissions of the same or alternate URLs can result in being banned from the directory.
Tips for Faster Acceptance:
- Location must be shown, preferably by address or local phone number.
- For title, submit actual name of the lodging, park or attraction that is the subject of the site.
- Make description factual, avoiding promotional language and Unnecessary Capitalization.
- Compare existing listings for best results.


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- Solid design
- Optically bonded display minimizes glare
- Lacks a MicroSD card slot
At A Glance
The best 7-inch Android tablet you can buy today, the Nexus 7 delivers solid performance and usability at a value price; but it also has at-times funky text rendering and its lack of a MicroSD card slot makes the 32GB model the better option.
Manufacturer's Description
Simple. Beautiful. Beyond Smart.
The Nexus brand represents a premium Android-based device that delivers the best Google experience available on tablet. It's the confluence of the latest software and state-of-the-art hardware and represents a literal nexus of digital life.
Nexus 7 is Google's latest Nexus experience device and the first tablet to feature Android™ 4.1 Jelly Bean. It combines the best of both Google and ASUS design, with the most advanced software from Google paired with cutting edge ASUS hardware.


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I created a Ubuntu Server 8.04 VM just for Zimbra, and during the installation I created the "root" user called Zimbra.
Is that why I am getting this error during installation?
I type sudo ./install.sh
it goes through and detects that no zimbra's are installed, then it gives the error in the subject of this post.
If I have to re-install ubuntu its no problem, just wondering if that is the cause or if i'm doing something else wrong.
I am trying to run the ./install.sh from a CD rom mounted from the client my VM is accessed through (but does not reside on)
Thanks for any help!!


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Kitchen-safe and made for use with messy hands. Includes Chef Stand, Stylus, and Stylus Holder.
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Wrap your HP Desktop Mini PC in the HP Desktop Mini Security/Dual VESA Sleeve to securely mount your PC behind your display, position your solution on a wall, and lock it down with the optional HP Ultra-Slim Cable Lock.
Lift your laptop to a comfortable viewing height and reclaim your desktop.
mStand provides better ergonomics, air flow and space management for laptop users, in Apple-matching looks and made of solid aluminum that matches Apple's MacBook Pro's silver-anodized finish.
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CTA Anti-Theft Security Case with Stand - Case for tablet - rugged - ABS plastic - for Apple iPad Air
Designed to support the HP Color LaserJet CM3530 Multifunction and CP3520 Printer Series. The sturdy, matching HP Printer Cabinet lets users optimally position the printer so it is highly accessible while adding storage for extra paper and supplies.
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The i360° 24-27" aluminum turntable provides an elegant and dynamic way to turn your iMac 24"/27" so that you can share ideas with others spontaneously.
Reclaim your space. mTower stand takes up only about a third of your notebook's footprint. Its solid single piece design gives the notebook the illusion of floating for a slick desktop setup (when using an external monitor as the single display).
Symbol CR0078-PC1F007WR - Docking cradle - Bluetooth - for Motorola DS6878, DS6878-DL, DS6878-HD, DS6878-SR
Introducing the mStand360 for businesses and professionals who require an elegant way to share the screen while enjoying stability.
The HP Adjustable Dual Display Stand allows you to use two displays to maximize your productivity and optimize efficiency.
This secure and sturdy shelf supports projectors, printers and other small peripherals. It easily attaches to the rear of the HP Presentation Station.
Ergotron - Cable management kit - gray - for Mobile WorkStand
Unitech - Bar code scanner desk mount stand - for MS 120
Enovate LITE is perfect in any environment – patient, emergency & operating rooms, labs, radiology, nurse stations - wherever patient data or a simple mobile worksurface is needed.
The NEW VX mobile computing cart line offers maximum flexibility and a superior features / value relationship to make sure you get the most out of your computer cart program budget.
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Working in a home office and regularly using a laptop doesn't mean you have to work uncomfortably or risk overheating your laptop. IR1360 notebook stand provides you with an secure, easy way to take advantage of limited desk real estate.
Increase the usability and convenience of your iPad, Galaxy or other table device with the Universal Tablet Stand from Laptop BuddyT.
Tablet Mount from Crane Hardware uses a specially designed extruded aluminum clamp and a secure tablet mounting claw that enables you to attach your tablet to a table surface, rigid gear case, mic stand, and of course your Crane Stand.
Ultra-portable workstations built around Apple's new MacPro and Thunderbolt 2.0, provide the power of the post-house in a single carry-on sized box.
The MagConnect Wall/Cabinet Mount from The Joy Factory attaches securely to flat surfaces for permanent installation. Experience the joy of worry-free mounting with a Wall/Cabinet mount with a rugged, water-resistant case from The Joy Factory.
Heavy Duty Universal car seat headrest mount specially designed for your tablet. Bracket fits most 7in to 10in tablets. Mounts easily between passenger and drivers seat.
Luxor LAMC's Adjustable Height Mobile single person computer desk are made from heavy-duty gray wood laminate.
Black Box - Cabinet unit for 20 tablets / 20 notebooks - steel - gray
Enjoy the most adjustable mount in the industry with a MagConnect Clamp Mount. This impressive mount attaches to flat surfaces, and extends two carbon fiber arms to mount your tablet in a wide variety of settings.
More and more artists are experiencing the benefits of using a tablet for creating their artwork. It is perfect for both indoor and outdoor mobile photography and videography.
When you buy selected software from MacMall, you have two delivery options: you can have it shipped, or you can download it straight to your PC. Downloadable software consists of two parts: 1) a product key and 2) the software files. Both are provided once your order has been processed. Download time will be dependent on your connection speed.
Applies only to items marked as "Same Day", and which do not require configuration, and which are available for delivery in the contiguous United States. The "Same Day" indicator is not a guarantee that your order will ship the same day you place it. This only indicates that the item in question was located in a warehouse at the time the website was last updated, and that we may be able to ship the product to you today if it is available pending credit approval and receipt of your order at the warehouse-if all applicable guidelines are met
This indicates that the product was located in a warehouse at the time the website was last updated, and that the warehouse is closed for shipping at this time, and that we may be able to ship the product to you on the next business day. Your order will usually be shipped the next business day if it is available, pending credit approval and receipt of your order at the warehouse-if all applicable guidelines are met.
This product is not currently available for shipment from any of our warehouse locations based upon information available to us at the last time the website was updated. Please call us at (800) 622-6255 for an estimated arrival date.
Product availability information presented on our Web site is the best, most current information we have, but errors do occur and this information does not represent a guarantee or promise of availability.
If a product is not in stock when you place your order, we will do our best to let you know. We will also provide you with our best estimate of when the product will be available. Anticipated delivery dates are dependent on our suppliers and other factors beyond our control, and are subject to change.
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MacMall is the #1 Apple Direct Reseller. We sell more Apple-branded products than any other direct Apple reseller. Since 1987, MacMall has been supplying Mac computers, iPad, Mac software, iPods, iPad accessories, and more Apple brand products and consumer electronics to consumers, businesses, and creative professionals. At MacMall, we understand the increasing demand for the best quality consumer products at huge discounted prices. That's why we are proud to offer the lowest prices on our extensive selection of best-selling products, and hard-to-find Mac and iPod products you crave. Get yourself the new iPad or the new Apple TV for your family. Looking to upgrade your MacBook? Or just want to find out the best suited iPod/iPhone accessories for your hot gadget? Keep your iPad up-to-date with function and technology with our line of top-brand iPad accessories. Compare brands. Equip your Apple products. More than the innovative products, MacMall goes beyond commerce and into affordable, high-quality business solutions. From customized business sites to financing & leasing to software licensing, technology certifications, configuration services, packaged service programs, and more. Enjoy easy management, easy installation, and easy implementation. Discover a whole new way to create and re-create your business. Plus, with our 24/7 customer service hotline, we're always just a phone call away no matter when you want to reach us. Get the MacMall Advantage. Don't miss out on the best deals, special prices, rebate savings, exclusive bundles, and dedicated service you won't find anywhere else!


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God I hate Sony. So I just bought Tokyo Jungle, yet I can't download it!
It said "activating...please wait" for a minute, then gave me the 80029780 error. After that it just gave me that error after 1 second of clicking download.
I transferred the account to another email address, which is the common solution. Sadly same thing happened, 1 minute of wait then an error.


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Two emergency room nurses and an emergency room receptionist at the Grand River Hospital District have teamed up to promote safer driving habits among Garfield County teens.
With support from the hospital, Lisa Domer, Lynda McFarland and Darnelda Counts have created a comprehensive, ongoing program, "Don't Get Wrecked," which addresses multiple aspects of safe driving and encourages teens to sign a pledge to practice safe driving techniques: Wearing a seat belt, not drinking and driving, not texting while driving, etc.
"It's been proven that programs like driver's ed decrease the number of accidents," McFarland said, but noted driver's education is a short-term program. Other programs, like the Colorado State Patrol's "Click-it or Ticket" program are ongoing, but only address one topic.
The district's program, which offers education and information, encourages teens to sign a pledge that they will not participate in any unsafe driving practices, and that they will promote safe driving as passengers.
"Thirty-five percent of teen deaths are related to car accidents," McFarland said. Tasks an adult driver with more experience might be able to manage can prove fatal for a teen driver.
In 2010, nearly 3,100 people were killed in crashes involving a distracted driver in the U.S., and another 416,000 people were injured in crashes that involved a distracted driver, according to figures from the district's program.
Working together with the Rifle Police Department, and with educators and students at Rifle High School, the program has also received support from local businesses, which have provided prizes, such as gas cards, for teens who sign the pledge.
"We're hoping that the more prizes we can offer, the more kids will participate," McFarland said.
Parents are asked to sign the pledge, too.
"Kids augment their parents' bad driving behaviors. If dad goes 80 mph, his son will go 82 mph," McFarland said.
"I really appreciate that this program gives the kids an opportunity to make this pledge on their own, it's not just somebody nagging at them to put on their seat belt and not text and drive," said Counts.
The group has created one informational video about the effect of texting on one's driving ability, with help from Spruce Creek Multimedia and Altitude Driving School. The video, the first of several they plan to produce, will be shown at Rifle High School in the spring, and eventually posted on local television and the Internet.
McFarland said they hope to take the program to students at Coal Ridge High School as well.
"If we can reduce the number of accidents in Garfield County with this program, if we can save one life, it's worth it," McFarland added.
For more information on the "Don't Get Wrecked" program, call Grand River Hospital District at 625-6451.


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NYC Condom Media Campaigns
Using a condom every time you have anal, oral or vaginal sex protects you and your partners from getting HIV and other sexually transmitted diseases (STDs) such as chlamydia, genital herpes, hepatitis B and syphilis, and prevents unplanned pregnancies.
Although nothing except not having sex provides 100% protection, condoms are approximately 95% effective in preventing STDs and provide protection against pregnancy. But better safe than sorry—use another form of birth control (such as the pill, IUD, diaphragm, Depo-Provera) and a condom for greater prevention against both pregnancy and STDs.
Birth control methods such as the pill, IUD and diaphragm alone do
not protect you from getting HIV and other STDs.
Take the worry of disease and pregnancy out of your sex life - using condoms can make sex more relaxed and enjoyable. You can get NYC Condoms free of charge.


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I know people always brag on this forum about how they passed a guy on a $10,000 carbon fiber bike wear a full kit, aero helmet, shoe covers, shaved legs, going up a hill on their 45 pound extracycle carrying a trailer without sweating or losing their breath and how good they feel and what not.
I had a funny incident yesterday where I was the roadie! I generally feel like I cross the line between many types of cyclist. I commute to work on a cyclocross bike with rack, lights, panniers, etc. I have a relatively nice road bike and a really enjoy riding road. I also do triathlon, cyclocross, and am starting to get into mountain biking.
Yesterday I rode a bike for the first time in over a week because I've been home sick with the flu. I just got my caad-10 road frame warrantied because it cracked and I really wanted to try out my new bike! Early in the ride I passed a dude on an old 10 speed in baggy pants and a hoodie with no helmet. I said hi, and passed him going approx 16mph up a slight but long grade. For the next 5-10 minutes I could tell he was trying to re-pass me (and of course it made me think of this forum). Eventually we came to a stop light and I slowed to a stop just as the light turned green. The timing was perfect for him to catch up and zoom past me (without saying hi!), ha ha. Right after the light was a long downhill and he was pedaling like crazy. I caught up to him at the next traffic light and we had a nice conversation riding side by side for the next few miles before our routes went different ways.
Roadies and Commuties can be Friendsies guys!!


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KLEM Local Schedule (week of July 1)
MONDAY RESULTS - July 1
(high school softball)
(#1-4A) Le Mars 4, Estherville/Lincoln Central 0 (RECORD: 29-and-0 / 12-and-0)
(#2-1A) Akron-Westfield 12, Gehlen Catholic 2 -- 6 innings (RECORD: 11-and-16 / 7-and-11)
(ESTHERVILLE) -- Top-rated (4A) Le Mars won their school-record 29th win of the season and in so doing wrapped up the Lakes Conference title with two games to play in the regular season, winning 4-0 over Estherville/Lincoln Central Monday night in Estherville. The Lady Bulldogs took a 3-0 lead in the first inning on a two-run single by Mary Kate Eckstaine and a RBI double by Molly Renken. Holly Schneider's sacrifice fly in the sixth completed the scoring. The rest of the night belonged to senior pitcher Molly Renken. A night before she celebrates her 19th birthday, Renken struck out eleven and threw her 17th shut out of the season while improving to 23-and-0. A fourth inning single to center by Emily Kirchner was the only hit for E-L-C. Le Mars improves to 29-and-0 for the season and 12-and-0 in the Lakes Conference. The Lady Bulldogs host Emmetsburg Wednesday and Spencer next Monday to close out the regular season.
(LE MARS) -- Kayla Tindall checked Gehlen Catholic to three hits in four innings and combined with Dana Hedlund on a six-hitter as number-two (1A) Akron-Westfield beat Gehlen Catholic 12-2 in six innings Monday night at the Riverview Complex. The victory wraps up the War Eagle Conference title for the Westerners, who go to 18-and-0 in the conference and 29-and-2 for the season. Kourtney Hedlund led a ten-hit attack with three hits and two RBI's. Bailey Davis had two hits and two RBI's. Shannon Smith had two hits for Gehlen. Abigail Krienert drove in both Gehlen runs in the sixth to avoid a shutout. The Lady Jays fall to 11-and-16 for the season and 7-and-11 in the War Eagle.
(high school baseball)
(#6-3A) Le Mars 11, Estherville/Lincoln Central 10 (RECORD: 20-and-3 / 11-and-1)
Gehlen Catholic 9, Akron-Westfield 6 (RECORD: 10-and-11 / 8-and-9)
(ESTHERVILLE) --The sixth-rated (3A) Le Mars Bulldogs rallied with five runs in the sixth inning to pull out an 11-10 Lakes Conference road victory over Estherville/Lincoln Central Monday night. The win clinched at least a share of the Lakes Conference title and the Bulldogs can wrap up their third straight conference championship at home Wednesday against Emmetsburg. Jacob Lamoreux and Dayton Rand paced a nine-hit attack with two hits each. Rand drove in three runs. Caleb Cline, Jacob Lamoreux and Colan Borchers pitched for Le Mars. Lamoreux earned the win and Borchers got a save. The Bulldogs are 20-and-3 for the season and 11-and-1 in Lakes Conference play.
(LE MARS) -- Gehlen Catholic won their fourth straight game, 9-6 over Akron-Westfield Monday night to improve to 10-and-11 for the season and 8-and-9 in the War Eagle Conference. A four-run fourth inning helped the Jays break a 3-3 tie after the first three innings. Blake Wiltgen won his first game of the season after an 0-and-6 start, scattering seven hits through five innings with five walks and five strike outs. Michael Whitehead finished the final two innings to earn a save, striking out four batters. Mitchell Flack led the offense with a pair of doubles among his three hits and three RBI's. Austin Herbst had two hits, including a triple, and drove in two runs.
TUESDAY RESULTS - July 2
(high school softball)
Hartley-Melvin-Sanborn at Gehlen Catholic - POSTPONED
(high school baseball)
Hartley-Melvin-Sanborn at Gehlen Catholic - POSTPONED
(American Association baseball)
Gary Southshore Railcats at Sioux City Explorers - POSTPONED
WEDNESDAY RESULTS - July 3
(high school softball)
(REMSEN) -- Miranda Staab and Katie Arens combined to no-hit Gehlen Catholic as number-eight Remsen St. Mary's beat the Lady Jays 11-1 in six innings Wednesday in a War Eagle Conference softball game at Remsen. The Hawks tagged Jill Kessenich and Brittany Krienert for eleven hits with Mackenzie Ricke, Paige Harpenau, Miranda Staab, and Alyssa Trejo collecting two hits each. Staab and Trejo drove in three runs apiece. St. Mary's scored seven runs in the third inning to break the game open early and brought an end to the contest with a run in the bottom of the sixth for a ten-run rule verdict. Bailey Wiltgen's RBI ground out in the fourth inning provided Gehlen's lone run to avoid the shutout. Gehlen ends the regular season at 11-and-17 and 7-and-12 in the War Eagle Conference. Gehlen hosts River Valley Monday in the 1A Regional opener at the Riverview Complex starting at 7 p.m. Coverage on KLEM begins at 6:50. Remsen St. Mary's takes a 24-and-6 season mark into the 1A Regionals next week and finished in second place in the War Eagle Conference race at 17-and-3. The Hawks have a first round bye and open tournament play next Wednesday at home.
(#8-1A) Remsen St. Mary's 11, Gehlen Catholic 1 -- 6 innings (RECORD: 11-and-17 / 7-and-12)
Emmetsburg at (#1-4A) Le Mars - CANCELLED
(high school baseball)
Emmetsburg at (#6-3A) Le Mars - POSTPONED TO TUESDAY, JULY 9th
(American Association baseball)
(game one) Sioux City Explorers 4, Gary Southshore Railcats 2
(game two) Gary Southshore Railcats 4, Sioux City Explorers 0
THURSDAY RESULTS - July 4
(American Association baseball)
Sioux City Explorers 5, Gary Southshore Railcats 3 -- 12 innings
FRIDAY RESULTS - July 5
(American Association baseball)
Sioux City Explorers 6, Gary Southshore Railcats 1
SATURDAY RESULTS - July 6
(high school baseball)
Sergeant Bluff-Luton 12, (#5-3A) Le Mars 4 (RECORD: 20-and-4)
(LE MARS) -- Unrated Sergeant Bluff-Luton took advantage of nine errors to score eleven unearned runs and the Warriors beat number-five Le Mars 12-4 Saturday night at the Bulldog Sports Complex. Le Mars committed five errors in the first two innings to allow the Warriors to jump out to a 6-1 lead. Jacob Lamoreux had a RBI single in the fourth followed by a two-run hit by Hunter Rowe to pull the Bulldogs to within 7-4. But four more errors in the fifth led to five more runs for S-B-L and the game was never in doubt again. Sergeant Bluff-Luton roughed up Le Mars ace Colan Borchers for ten hits and twelve runs, although only one of the runs was earned. Matt Ellefson and Jacob Lamoreux had two hits each to lead a seven-hit attack for Le Mars. The Bulldogs fall to 20-and-4 for the season and return to Lakes Conference action Monday night at home against Spencer.
(little league)
Le Mars girls Junior All-Stars finished 2-and-1 in the Spirit Lake tournament. Le Mars lost 6-5 to Sergeant Bluff ... then won 5-4 over Denison ... and beat Spirit Lake 12-2.
Le Mars girls Major All-Stars finished 3-and-1 in the Spirit Lake tournament. Le Mars beat SWAG 17-4 ... then lost 9-7 to Crush ... won 13-1 over Okoboji and finished with a 9-6 win over Emmetsburg to finish in second place.
(American Association baseball)
Fargo-Moorhead Redhawks 8, Sioux City Explorers 2
SUNDAY RESULTS - July 7
(little league)
Sergeant Bluff 15, Le Mars 11 & under boys All-Stars 2
Sergeant Bluff 5, Le Mars 12-year old boys All-Stars 1
(American Association baseball)
Sioux City Explorers 6, Fargo-Moorhead Redhawks 4


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Burrowing owls call Phoenix office home
Western's customer service territory shares the land with a great diversity of wildlife. Sometimes we even share the borders of our office buildings' grounds with fascinating and interesting creatures. In the Desert Southwest region's Phoenix office, employees work while 10 to 15 burrowing owls live just outside their door.
The convenience of being near wildlife brings some happiness to employees. "We all enjoy having [the owls] here at DSW. We have this great opportunity to be close to the wildlife and observe their living habits," said Supply Technician Mary Bergeron.
As a result of an increasing human population leading to more residential and commercial development on agricultural land and prairies, wildlife species like the burrowing owl lose their natural nesting areas. For safety, health and conservation reasons, displaced owls are often relocated to man-made nesting structures like the one at the DSW yard.
In 2004, the Arizona Game and Fish Department conducted a study to determine the effectiveness of relocation efforts of burrowing owls to artificial burrows. They also wanted to establish relocation guidelines and recommendations for future management plans. As part of this study, AGFD monitored up to 50 relocation clusters throughout Arizona, including the one at DSW. On a regular basis, AGFD biologists would collect data on owl behavior, confirm the new burrow system was working, place bands on young birds to track their movements and make sure the site was still suitable for the owls. DSW's artificial owl burrows are constructed of a PVC pipe system that is buried underground.
Unfortunately, the funding for this project ended after 2007, and the AGFD has not monitored the DSW birds for several years. Even so, DSW's feathery neighbors are living happy lives today. If a funding source is secured in the future, the AGFD would like to continue monitoring the birds.
Learn more about burrowing owls.


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Whether you've injured your back playing golf or wake up in the morning with mysterious neck pain, injuries and activity-related conditions can be annoying and often debilitating.
Sports Medicine and Injury Care at Carolinas HealthCare System, with locations throughout the Charlotte, NC, metropolitan area, will schedule an appointment within one business day at one of our eight locations.
Carolinas HealthCare System offers a dedicated network of experts who treat a range of spine, muscular, or sports-related conditions for adolescents, adults and seniors. Our integrated team combines the services of board certified sports medicine, interventional spine and physical medicine specialists into one comprehensive program with eight convenient locations.
We want to make sure you regain your quality of life as quickly as possible. That's why our patients are always treated by a board-certified specialist - no matter the condition.
A full range of experts are available in convenient locations so access to care is never far from home. Same day appointments are available. Call 704-863-HURT (4878) to make an appointment today.
Common sports/spine injuries and conditions:
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zmcontrol -v
Release 7.2.0_GA_2669.RHEL6_64_20120410002025 CentOS6_64 FOSS edition
I have a weird issue on my zimbra server. I have a setup with multiple backend servers and a seperate proxy and LDAP master. For 2 years everything has been working fine, but lately I've noticed some users having issues with spell check in zimbra web. I am having a hard time pinning down the problem. The users affected seem to be a random collection from different servers and different domains. So for example email@example.com can spell check, but firstname.lastname@example.com can not spell check even though they are on the same backend server and in the same domain/COS
If anyone has any ideas where i need to look to troubleshoot this issue, that would be great.
Let me know if you need anymore information from my side.


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M-212S - TUSA Ceos Pro Mask
The M-212 Ceos is a low-profile low volume two window mask that features the newly developed and revolutionary Freedom Technology delivering superior fit, comfort and performance. The Ceos is one of the first next generation TUSA masks offering this innovative new technology combined with superior design, build quality, materials and frame construction.
In addition to Freedom Technology, the M-212 features TUSA's patented Quick-Adjust Buckle System, 3-D Anatomical Strap, Round-Edge skirt technologies and is available in a PRO version (M-212S) with CrystalView optical glass lenses treated with a proprietary anti-reflective (A/R) and ultraviolet (U/V) coating.
> Low profile, low-volume 2-window teardrop design
> Equipped with Freedom Technology
> Skirt mounted Quick-Adjust Buckle System
> Round-edge skirt for maximum fit and comfort
> 3-D strap for ideal anatomical fit
> Accepts MC-7500 Corrective Lenses and ML-7500S AR/UV lenses
> PRO Version (M-212S) with CrystalView AR/UV lenses
> Colors: Black (BK), Cobalt Blue (CBL), Flash Yellow (FY), Pearlescent Pink (PP), Siesta Green (SG), Translucent/Black (T/BK)
> Black Silicone: Black (QB-BK), Metallic Dark Red/Black (QB-BK/MDR), Siesta Green/Black (QB-BK/SG)
> PRO Version (M-212S) with CrystalView AR/UV: Black (SQB-BK), Metallic Dark Red/Black (SQB-BK/MDR)
Freedom technology is a set of revolutionary features offering superior fit, comfort and increased performance in TUSA masks. Freedom model masks feature a dimpled skirt surface with varied silicone thicknesses and stability ridges in addition to a proprietary low-friction skirt surface on the fitting line.
CrystalView Optical Glass
CrystalView optical glass lenses provide superior clarity, color and transmission of light over standard glass lenses used by the competition. Up to 15% of available light can be lost with a standard glass lens due to impurities and reflectivity. TUSA's CrystalView lenses transmit 92% of available light to the diver for clear crisp vision. CrystalView lenses are used in all PRO models masks and also in the M-41 Freedom Quad.
UV Lens Treatment
Increased exposure to UV light can damage the eyes especially in marine environments where reflectivity is high. TUSA's UV Lens Treatment provides 100% protection from harmful UVA and UVB rays. Blocking 100% of the ultraviolet spectrum (under 400nm) provides greater eye protection without sacrificing light transmission or vision.
Anti-Reflective (A/R) Lens Treatment
Standard glass lenses can reflect up to 15% of available light reducing vision underwater. TUSA's Anti-Reflective (A/R) Lens Treatment decreases internal and external reflected light. This reduction increases light transmission to 95% resulting in significantly enhanced color, clarity, contrast and better overall vision underwater.
Skirt Mounted Quick-Adjust Buckle System
The Quick-Adjust Buckle System incorporates a newly-designed low-profile buckle into the crystal silicone skirt of the mask. The result is a compact, lightweight, technologically advanced mask design that can be easily and quickly adjusted for a perfect fit.
Round Edge Skirt
TUSA's Round Edge Skirt features a proprietary round edge cross-sectionally shaped skirt. This rounded edge results in a soft fit that suppresses pressure and reduces creasing left on the face. The 'planar fit, instead of linear fit' silicone skirt maintains great fit and unprecedented comfort.
3-D Strap
TUSA's patented 3-D strap is a true three-dimensional design that snugly fits against the head's natural curvature. The 3-D strap offers unprecedented comfort and fit unlike standard flat mask straps.
Mask, Snorkel and Fin Care
Q: Should I clean my new mask?
During the production process silicone film can form on the glass lenses which can cause increased fogging. A toothpaste or a very mild abrasive liquid can be used to gently scrub the inside of the lens to remove this silicone layer. This will help to reduce excessive fogging on a new mask.
Q: What causes my mask to fog, is something wrong?
As mentioned above when new it is typically the silicone film from production. However, over time oils and contaminants can build up on the lenses causing a layer on top of the lens that will increase fogging. We recommend cleaning the mask occasionally with a mild liquid soap or detergent to remove this build up. This can help to reduce fogging that may occur with a dirty lens.
Q: How do I stop my mask from fogging?
Aftermarket anti-fog solutions are available and can be used on TUSA masks. Companies such as JAWS, McNett, and 500psi amongst others offer suitable products. Please contact your local TUSA retailer for their recommendation.
Q: I have CrystalView AR/UV lenses in my mask, any additional care required?
Masks such as the M-211 Freedom One PRO and M-28S Geminus PRO are equipped with CrystalView AR/UV lenses. These optical glass lenses are treated with a multi-layer proprietary anti-reflective and ultraviolet coating that is hardened for durability. Although not likely, care should be taken when using these lenses as the coating could possibly be scratched or removed. The coating is on the outside of the mask lens, which allows cleaning and use of an anti-fog solution on the inside without issue. Take care to read the included care and maintenance information with each CrystalView AR/UV equipped mask.
Q: How should I care for my mask, snorkel and fins?
After use rinse your equipment in fresh clean water and dry thoroughly before storing. Take care that any purge valves or snorkel tops are free from sand and debris that can cause leakage. Store in a cool dry place away from heat and sun. Do not dry your equipment in direct UV light for extended periods of time.
Q: Can I get parts for my Mask, Fins or Snorkel?
TUSA offers a variety of straps, buckles and accessories for replacement if a part is broken or damaged. These are available as TUSA CARE genuine accessories and must be purchased from your authorized retailer.


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Ned Yost media session: June 17
Georgia on my mind
Yost thinks it will be fun to play in Atlanta and looks forward to seeing his former boss, Bobby Cox. Yost spoke very highly of the Braves manager, saying that Cox is a very special person who doesn't really even compare to anyone else.
Hey now, you're an all-star
Having been to four All-Star games himself, Yost thinks the 2012 All-Star game will be a wonderful time that all of Kansas City can enjoy. He believes that a top-notch ballpark like Kauffman will without a doubt be the perfect venue for such a once in a lifetime experience–both for the spectators and the players.
Get your roll on
Yost also discussed the bullpen's improvements, which he credits to the players' confidence in and understanding of their rolls. He is especially pleased with Kyle Farnsworth who has pitched "spectacular" and has been on a "real nice run." Yost would, however, like to get Joakim Soria in more games.
Hurt so good
Gil Meche is making progress and threw 10-15 pitches off the mound for the first time since being put on the DL due to bursitis in his pitching shoulder. Additionally, Luke Hochevar, who was placed on the 15-day DL retroactive to June 12, feels much better and was symptom free yesterday.


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Ormet Workers Watch and Wait
Monday was final day for comment on pending case
September 10, 2013
HANNIBAL — Only the five members of the Public Utilities Commission of Ohio can decide whether Ormet Corp....
The school tax issue should not be an issue. It was stated recently that Monroe Co. has the most productive gas well in the state. Producing gross money exceeding $300,000 per day. I'm sure the school systems can tap into some the gas well money to make up for any lost money from ormet property tax. the property tax on all the gas lines alone should be enough.
1 Agrees | 1 Disagrees | Report Abuse »
Truth is there is a billion dollar processing plant being built now on Monroe co. And many wells being drilled. Big, big money floating around. Big, high paying jobs also. But, but the employees must Work long hrs. If ormet's costs are too high, then cut them. Or raise price of products, or close doors. Or do what GM did. Get Obama money?
1 Agrees | 1 Disagrees | Report Abuse »
I say give them a break...let them run!
1 Agrees | 1 Disagrees | Report Abuse »
Where is this billion dollar processing plant being built LogHog? Provide us with some details.
2 Agrees | 1 Disagrees | Report Abuse »
LogHog showed his knowledge on the matter... raise the price of the products (he obviously knows nothing about the metal exchange or how prices change due to global demand). Also, cutting costs (that's what the hearings are designed to do... cut costs). And this Billion dollar processing plant is being built where??? Oh yeah, he never responded to that question. A natural gas fired power plant is being planned to be built... On Ormet property utilizing the gas that is being drilled... on Ormet property. And AEP is going to continue to raise rates on it's customers because they have been granted the authority by PUCO so they can meet obligations to their stockholders. Don't forget, they made their stockholders $1.3B last year and $1.9B in 2012.
2 Agrees | 2 Disagrees | Report Abuse »
AEP has "donated" 5.6 million dollars to politicians since 2004, 93% of which has been given to the incumbent! Keep on thinking this is the "desired" political system that the counties of the World want.......
2 Agrees | 0 Disagrees | Report Abuse »
That is correct. AEP made a profit for its' shareholders (owners) like all businesses should.
It is not AEP's fault that Ormet cannot run their business. Blaming the power company? C'mon!
Maybe I can try that. Open a car wash and then demand that the power and water come to me at dirt cheap costs, with my neighbors making up the difference.
If you cannot sell your product for more than it costs you to produce it, you lose money. If you lose enough money, you go out of business.
Remember, Ormet wasn't exactly churning out big profits when they were getting electricity at a massive discount.
If you can't make it in business, get out of business.
1 Agrees | 2 Disagrees | Report Abuse »
Since 1998, the United Steelworkers of America has spent more than $6 million on lobbying.
In the 2011-2012 election cycle, the USWA donated $3.12 million to Democrat politicians and PACS.
More than 60,000 steelworkers lost their jobs while Clinton was President yet, Bush gave them the tariffs that they demanded and they STILL give 100% to Democrats?
Since 2004, AEP has donated $66,828 to political causes, with $0 spent on lobbying.
In 2012, AEP paid $604 million in income taxes and $915 million in dividends, in addition to employing 18,000 Americans in good paying jobs AND buying millions of tons of coal, thus supporting coal jobs.
AEP's shareholders are widows, pension funds, retirement funds, mutual funds and investment banks, along with tens of thousands of small time investors who like the security and 4% yield.
If Ormet goes kaput, it will cause a little pain.
If AEP went out of business, it would affect millions of Americans.
1 Agrees | 2 Disagrees | Report Abuse »
Why does everyone want ormet to shut down? If ormet gets this help...they will create jobs when they build the power plant. Aep won't have to worry about ormet then.seems everyone thinks that ormet is causing the increase in power rates but when ormet shuts down. ..watch the rates go up anyway.I know they will sell the power somewhere else but untill then I guarantee the rate will still go up.probly more than if ormet would run.people we need to keep these jobs! Sheesh!
1 Agrees | 2 Disagrees | Report Abuse »
rangar, AEP has a multiple PAC's! That is the "lobbying" arm. My point we have the best goberment that money can buy. I doubt that the Union/Company workers in any business have a clue who they support with dues paid......
0 Agrees | 1 Disagrees | Report Abuse »
I don't think anybody realizes that Ormet is paying A LOT MORE for electricity than ANY other aluminum smelter in the country and ANY other residential customer. The problem is in 2009 the rates started to increase. Every time AEP "gives" a reduction to any customers, AEP has the PUCO charge somebody else for it. There is a 5 million dollar charge per year to big industry for AEP's hot water tank program. Ormet is not asking for a favor, they are simply asking for the going rate - plain and simple.
1 Agrees | 2 Disagrees | Report Abuse »
jessie, What is the going rate?
1 Agrees | 0 Disagrees | Report Abuse »
I for one wonder how AEP is going to ask for rate relief when Ohio is going deregulated. Full dereg by June 1, 2015
1 Agrees | 0 Disagrees | Report Abuse »
Jessie, If Ormet wants the going rate why don't they just get it? The going rate today was in excess of $260 per mwh. Check out PJMs website. Then check your "facts".
1 Agrees | 0 Disagrees | Report Abuse »
From the energy collective: "Wholesale electricity prices briefly touched as high as $230 per megawatt-hour in New England. Wholesale "nodal" prices across the PJM system, which spans from the mid-Atlantic states westward through Ohio, West Virginia and parts of Indiana, Kentucky and Illinois, also ranged from $150 to $200 per megawatt-hour. For comparison, typical wholesale prices frequently range from $35 to $60 per megawatt-hour." Check your electric bill and tell me what your rate is. Ormet is paying well above the highest typical rate indicated. Check YOUR facts!
1 Agrees | 1 Disagrees | Report Abuse »
Summerfield, Ohio... easy to see from the old school grounds
0 Agrees | 0 Disagrees | Report Abuse »
AEP owes nothing to Ormet. Ormet wants to get reduced rates from AEP until Ormet builds it's own power plant to then cut out buying from AEP. Does this sound like a sound business plan for AEP?
0 Agrees | 0 Disagrees | Report Abuse »
I have nothing against Ormet. If they can pull a rabbit out of the hat and keep running while loosing money, go for it. Just don't make my grandma pay any of their electric bills... none... she is on a fixed income
0 Agrees | 0 Disagrees | Report Abuse »
thanks nightmare...maybe I should buy some AEP stock... is that your 1st name or your real name?
0 Agrees | 0 Disagrees | Report Abuse »
Loghog writes: " I'm sure the school systems can tap into some the gas well money to make up for any lost money from ormet property tax. the property tax on all the gas lines alone should be enough."
I'm not going to do your research for you, so you'll need to justify your statements with some facts"
0 Agrees | 0 Disagrees | Report Abuse »
Ormet, when (if) it can be profitable does something that many local business's cannot do. That is, since it is directly involved in the global metals market, it can transfer global wealth to the Ohio Valley. That is not a bad thing, since it benefits the region as a whole. Stop, and think about this for a minute.
Loghog writes about the development of the gas industry in the region, and that is a positive development. In fact, Ormet is seeking to use that natural resource as the fuel to power its smelter. It seeks to free itself from ratepayer subsidies and be self sufficient, as it should be. In my opinion, I believe we should support those industries that, in the past made America great. We have seen the demise of the steel industry and the attacks against the coal industry, weakening a once great nation. Without them we could not have defeated the Axis in World War II. The Ohio Valley along with Pittsburgh, Cleveland, Detroit and many other cities and towns built this nation,
0 Agrees | 0 Disagrees | Report Abuse »
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I have a feeling that if she gets this job we'd likely live in Colorado Springs area. I may have a lead on a place to look for a job for myself, but her getting the position she needs is the tough part and we could end up almost anywhere in the country and will find out in December.
I've been dreaming of doing more western hunts but the cost and distance are limiting factors right now. I know archery hunting would be vastly different but I'm looking forward to it. I tried to draw CO archery mule deer this year and didn't get it. Was debating ID or CO for OTC archery, but my dad is older and I'd rather take the time to go fishing with him than hunting by myself so MT fly fishing it is in September.
If we did end up there I think I know the waterfowl hunting wouldn't be as good, but maybe some upland hunting? My lab is hitting her prime years and I like to get out with her when I can. We've been to SD for pheasant the last two years as a family.
The deal is if we move it'd likely only be for the 3 years of her training and then unless she likes it we are likely to move again. Pueblo may be hot but that might be one of the things that sells her. She wears a sweatshirt at room temperature. And she considers an area rural unless the nearest town has a Macy's (don't ask how we met and got married, sometimes I wonder myself). Haha what can you do she was raised in north jersey.
All I know is the housing has to be more affordable than here. About the only areas worse are NYC metro, DC, and LA.
I'd sure be excited to move west but it'll be hard to learn all new areas and frustrating to know we'd likely move again in 3 years and I won't be around long enough to take advantage of the resident status for some of the premium draws.
I may ask some more questions. We'll see how her interview goes.


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this dress is really really pretty
adoro a blusa com a gola as bolinhas
wow, it's such a pretty dress, i want it
very delicate white dress. so lovely
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Optimization of Human Embryonic Stem Cell Derivation Techniques and Production/Distribution of GMP-Grade Lines
The government has strict rules for producing cells that will be transplanted into patients. For example, these regulations discourage the use of animal products that could transmit diseases to humans. In this context, the high-quality and tightly regulated procedures that govern other cell-based therapies, e.g., bone marrow transplants, will be applied to regenerative-type clinical applications that employ human embryonic stem cells (hESCs). We need to produce these cells now so that they will be ready to use when research findings are translated into patient therapies. Our goal is to supply researchers in California and outside the state with the highest-quality hESCs. To achieve this goal, we will build on our previous work, published in the scientific literature, which includes deriving hESCs from intact embryos and their single-cell components. We also study the basic properties of embryos and hESCs so that we can formulate theories about how to improve the derivation process, which we then test in our laboratory. For example, adult humans need very precise levels of oxygen. Our work shows that the same is true for embryos and hESCs. We have also developed novel culture conditions that use defined components such as those that are required by the governmental agencies that set the standards for the production of cells used in therapeutic applications. Accordingly, we propose a two-phase approach. During the first two years, guided by advances made by our group and others, we will derive hESCs from embryos in a biologically relevant oxygen environment using defined, high-quality materials. We will also derive hESCs from single cells removed by biopsy from embryos at specific stages of development and/or from particular regions. We think that these lines might have more predictable properties than hESCs that arise randomly, the current practice. Thus, at the end of the first phase we will have produced and banked the next generation of lines, which will be derived under defined conditions that more closely comply with government regulations regarding the production of clinical-grade cells. In the second phase, year 3 of the project, we will use the conditions that best support hESC derivation/propagation to produce lines that can be transplanted into patients. Our efforts will benefit greatly from the infrastructure of our institution, which includes a government-approved facility for doing this work, and from colleagues with the requisite specialized expertise. With the resources provided, we think that we can generate and bank 12 to 20 cell lines; one-third will be produced in a manner that complies with government regulations pertaining to cell-based therapies. All will be widely distributed, as we believe that the pace of translational and clinical research depends on the availability of the highest-quality hESCs and on the important information about their fundamental properties that will emerge from this work.
California citizens were overwhelmingly in favor of proposition 71, due in large part to the public's belief that laboratory scientists working together with their clinical colleagues could develop therapeutic approaches that utilize human embryonic stem cells and their derivatives in regenerative medicine applications. The research teams are equally excited about cell replacement strategies for treating a variety of medical conditions, as in many cases a cure might be feasible. However, we know from the collective experience of the biotechnology industry that filling the pipeline that leads from basic research to clinical applications inevitably takes time. How do we start this process and shorten the timelines for delivering cell-based therapies to patients? Much of the work in the individual pipelines that focus on specific diseases/conditions can happen in a nonlinear fashion. For example, we need not wait until safe and robust strategies are devised for differentiating human embryonic stem cells into specific cell types to develop the lines that meet government regulations regarding the production of cells for transplantation into patients. There are many reasons that deriving these human embryonic stem cell lines now is crucial. For example, it is likely that production of the cells that will eventually be used in clinical applications will be an iterative process. That is, we will continue to make key discoveries about the fundamental properties of human embryonic stem cells, about which basic information is still needed to improve conditions for growing and deriving lines. This is particularly relevant to the production of clinical-grade cells, as it will be much easier to meet Food and Drug Administration requirements if cells are produced using defined materials that contain only human and recombinant components. It will also be important to know if clinical-grade cells, which for regulatory reasons must be derived under streamlined conditions, have the same properties as other human embryonic cell lines that are used for research purposes. Thus, extensive preclinical testing will be required before these cells are approved by the Food and Drug Administration for use in humans. Thus, accomplishing the major goals set forth in this application will be of enormous benefit to California's citizens. We envision that production of the next generation of human embryonic stem cells that can be used in clinical applications will speed the delivery of therapeutic applications to patients. Along the way these cells will have many other valuable applications. For example, they can be used to screen pharmacologically active compounds for both beneficial and detrimental effects. They will also be valuable tools for understanding the molecular etiology of disease processes. Accordingly, accomplishing the goals of this project will greatly benefit the people's health and California's economy.
The goal of our work is to derive new human embryonic stem cell lines for distribution to stem cell researchers. During the past year, we produced 11 new lines that we think will be valuable tools for all phases of work toward regenerative medicine therapies including discovery, translational, and eventually, early clinical applications. Along the way, this work is teaching us a great deal about early embryonic development and the specialized processes that distinguish critical steps in humans as compared to animal models.
What is the source of the embryos for our derivations? We use embryos that are left over after the conclusion of fertility treatments such as in vitro fertilization. In all cases, we obtain written informed consent from both individuals whose reproductive material was used to make the embryos. Additionally, researchers in our group have no direct contact with the couple making the donation. This process is handled by the University of California San Francisco Gamete and Embryo Bank. It is important to note that the embryos that we use would otherwise be destroyed, a point that is sometimes overlooked.
What are the methods that we used to derive these new human embryonic stem cell lines? The work that was carried out in the last 12 months employed two techniques. One line was derived using standard methods that have been employed for many years to produce stem cell lines in many species including humans. In general, early-stage human embryos are thawed and grown for a few days in the laboratory until a cluster of tightly packed cells, termed human embryonic stem cell colonies, emerges. Stem cell researchers can spot these special cells based on their appearance under a microscope. The advantage of this technique is that it is a straightforward method for reliably producing human embryonic stem cell lines. The disadvantage is that the progenitors we seek are exposed for extended periods of time to other cell types in the culture, which could impact their genetic makeup and, subsequently, their developmental potential.
Accordingly, we used a different method to derive the other ten embryonic stem cell lines that we produced during this grant period. The overall approach was to grow human embryos donated from a single couple until they contained 8-12 cells. Then we used specialized equipment to remove single cells from five sibling embryos. Each cell was allowed to develop in isolation. Four cells from one embryo gave rise to cell lines as did three cells from another embryo. The remaining three embryos each produced one cell line. This unique set of lines will enable stem cell researchers to understand the role that genetics plays in specifying the basic characteristics of human embryonic stem cells as compared to environmental signals that are inevitably transmitted as the lines are propagated in the laboratory. We are also interested in the concept that single cells that are extracted from very early stage embryos, which have not received signals from other cell types that are found at later stages of development, are more likely to be truly pluripotent as evidenced by their genetic and molecular signatures.
Why do we need additional human embryonic stem cell lines? Many of the most commonly used stem cells have been in culture for many years. This creates several problems. First, the extended length of time that the most commonly studied human embryonic stem cells have been in culture means that they are likely to have accumulated errors that are the result of mistakes made during the self-renewal process. This is all the more likely to occur because we do not yet understand the optimal conditions for growing the cells in the laboratory. Accordingly, ongoing studies in many groups, including our own, are designed to improve the environment for maintaining human embryonic stem cells, which we think will improve the fidelity with which they make carbon copies of themselves. Second, nearly all of the existing lines have been exposed to animal products, which raises the possibility that they could contain disease vectors. For this reason, the Food and Drug Administration makes it difficult (but not impossible) for cell-based therapies that are used in clinical applications to be approved once they have experienced these types of exposures. Therefore, all of our derivation work has employed solely human reagents, which we think will streamline their approval for use in clinical applications.
Our goal is to derive human embryonic stem cell (hESC) lines using new and improved methods. During the first two years of this project, we produced eleven new hESC lines. One line was derived from an intact six day-old human embryo that consists of 50-100 cells. Nearly all existing hESCs were derived using this approach. The other ten lines were derived using a new method that we helped to pioneer. This technique entails removing a single cell from an earlier stage human embryo that is comprised of 8 cells. Under the right laboratory conditions the cell makes copies of itself, a process that produces an hESC line. The advantage of this method over the more conventional approach is that we know when the founding cell was removed and where it came from. Additionally, the founder is isolated from the signals of other embryonic cells, which could erase aspects of the stem cell state. Finally, we derived multiple lines from individual embryos so subsets of the lines have the same genetic makeup, which will allow scientists to study the influence of the environment on basic stem cell properties.
During the current grant period, we registered the cells with the California Institute for Regenerative Medicine (CIRM). The process entailed submitting the paperwork that includes proof that the couples who donated embryos to this project gave informed consent. We also described the conditions we used for deriving and propagating these cells. Overall our methods emphasized the use of human materials, which avoids exposure to animal products that could be contaminated with infectious agents. These cells are now available for use by CIRM investigators and other scientists who have nonfederal research funds.
This year the National Institutes of Health (NIH) initiated a parallel process for registering hESCs. The goal was to greatly expand the number of hESC lines that investigators would be allowed to use in experiments that were paid for by federal dollars. To make the cells we derived with CIRM funds more widely available, we attempted to register our cells with the NIH, which required submitting paperwork that was very similar to the documentation CIRM required. The line derived by conventional methods was quickly approved.
However, the lines that were derived from earlier stage embryos have not yet advanced through the approval process. There were two problems. The first was that the Federal government defined an hESC line as coming from an embryo that consists of 50-100 cells. Our lines that came from 8-cell human embryos did not meet this criterion. Accordingly, the Government notified the public of the intent to change this definition. A comment period, which has now ended, ensued. However, the lines were still not approved. It then became apparent that a lawsuit contesting the use of federal funds for hESC research had been reinstated. Thus, ten of our lines are still listed as pending on the Federal Registry and we (and other investigators) cannot apply for (or use) Federal dollars to study them.
Our scientific progress included establishing a bank of the hESCs that we derived. The purpose of a bank is to stockpile cells at a particular stage for future use. The need for banks acknowledges that the basic properties of hESCs can change as a function of the amount of time they are propagated in the laboratory. To bank our cells, we expanded them to the point where we were able to store approximately a thousand vials that contained a million cells each. All were frozen after they had self-replicated only twelve times, which reduces the chance that untoward changes occurred. Thus, we are now in a position to distribute our cells to other investigators who want to use them in their work. Importantly, the size of the bank ensures that we and other investigators can return to this source to get additional vials of the same cells that had been cultured for the same length of time.
In additional experiments, we compared the properties of the ten lines that were derived from early embryos with conventional hESCs. In one series of experiments, we took a global approach in which we analyzed the fundamental properties of the cells. The results yielded data that suggested that the new lines had unique properties. We also found that lines that were derived from different embryos were more similar than lines that came from the same embryo, possible evidence that differentiation begins much earlier during the beginning stages of human development than was previously thought.
Finally, we asked whether the fundamental differences we observed between our new lines and conventional cells were mirrored at a functional level. These experiments are still in progress, but we have interesting evidence that supports this possibility. For instance, we found lines that came from the same 8-cell stage human embryo differed in their ability to make the kinds of neurons that are envisioned as cell-based therapies for Parkinson's Disease.
The goal of our project is to improve methods for generating and banking human embryonic stem cells (hESCs), which are derived from human embryos. Since the federal government does not support work with human embryos, this research would be impossible without the funds we have received from the California Institute for Regenerative Medicine (CIRM). Additionally, our project has benefited greatly from CIRM Facilities Grants, which provided us with this specialized nonfederal laboratories that we need for experiments that employ human embryos.
Our project has three Specific Aims. The first is to derive hESC lines from intact human embryos that have been grown in the laboratory for approximately five days. This is the method that has been used to derive the overwhelming majority of the existing hESCs. In these experiments, cells that are destined to become hESCs emerge over several days. Thus, the precise origin and timing are unknown. Some investigators think that this element of randomness contributes to the significant differences that have been noted among hESCs. To date, we used this approach to derive one line, UCSF-4, which was approved by CIRM. Thus, other grantees of this agency can work with these cells. Since they were generated using standard techniques, UCSF-4 was approved by the National Institutes of Health. Therefore, researchers who have federal funding can also work with these cells.
The second Specific Aim proposed a new approach for deriving hESCs. We wanted to make lines from single cells that were removed from embryos at very precise stages of development. To date we have focused on the eight-cell stage, embryos that have been grown for three days in the laboratory. In these experiments, we used five embryos that were donated by a single couple. In all, we established 10 lines from individual cells. In other words, we derived multiple lines from the same embryo. Thus, this collection is unique because some of the lines are genetically identical and all have a high degree of genetic relatedness. This special property is important to stem cell biologists as we are trying to understand the changes that happened over time as the cells are maintained in the laboratory. Additionally, we have evidence that hESCs that are derived from embryos that have been grown for three rather than five days are more plastic in that they are better able to form the precursors of a broader range of cell types that comprise the human body.
Like UCSF-4, the ten lines that were derived from single cells removed from embryos were registered by CIRM. They were also submitted to the National Institutes of Health along with UCSF-4. However, we were notified that they do not fit the federal definition of an hESC line, which specifies derivation from an embryo that has been grown for five rather than three days in the laboratory. Accordingly, these lines are still pending approval, which will require changing the federal definition. Therefore, it is uncertain if they will ever be registered, a process that allows federally-funded scientists to work with hESC lines.
Our third Specific Aim was to derive a hESC line using Good Manufacturing Processes (GMP) as specified by the Food and Drug Administration. These guidelines have been established to make sure that cell-based therapies are safe for patients. For example, they are designed to prevent the spread of infectious agents that can contaminate animal cells to humans. One obstacle to producing GMP-grade cells is that hESC derivation requires a carpet of another cell type, which is thought to "feed" embryos the substances that are required for generation of hESC colonies. Thus these "feeder" cells must also be produced using GMP methods. To avoid this laborious step, we have developed methods for the "feeder-free" culture of hESCs. We plan to use the same approach to derive hESCs from intact embryos.
To date, with CIRM support, we have derived 11 hESC lines. Therefore, distributing these cells has become a significant part of this project. It is interesting to note that these lines are being used for a wide variety of experiments. For example, UCSF-4 is being studied by a Consortium that has been formed to understand how chemical modifications to genes control their expression. This is a particularly interesting question because the programs that specify the initial stages of human development are very poorly understood.
The other groups who are using our cells are focusing on a wide range of normal and disease processes. Several investigators are studying the mechanisms by which cells of the brain and spinal cord develop. One group hopes to use them as a therapeutic strategy for patients with amyotrophic lateral sclerosis or Lou Gehrig's disease. Other investigators are studying formation of cardiac muscle, pancreatic beta cells and the liver. Finally, our lines are also being used for training purposes in our CIRM Shared Research and Teaching Facility.
The goal of our work has been to derive new human embryonic stem cells lines from embryos that are donated by couples at the completion of in vitro fertilization cycles. Many laboratories including our own, derived lines using the method that was originally described. Embryos that were grown for 5-6 days in the laboratory were transferred to a lawn of cells that supported the eventual outgrowth of human embryonic stem cells. Using standard methods to perform derivations, we were struck by ways in which we thought this method could be improved. For example, the cells of the embryo that are destined to form the placenta die. Therefore, it seemed likely that the products they release could have untoward influences on the stem cells. In addition, we observed the formation of morphologically distinct cell types before tightly packed "islands" of stem cells finally emerged in their midst. We speculated that these extraneous cells could also have undue effects. Finally, we examined, at very high magnification, embryos that were grown for 5-6 days. Although the cells that give rise to the offspring are supposed to be nearly equivalent at this stage, we saw that they no longer look alike. We took this as possible evidence of specialization, meaning they might already be starting down the road toward becoming one of the cell types of the body. Therefore, we began to consider new approaches for producing human embryonic stem cells. In particular, we wanted to derive lines from single cells of earlier stage embryos that might be less specified and more plastic in terms of their ability to differentiate into progeny that are difficult to obtain from conventional human embryonic stem cell lines.
In preliminary experiments, we worked with other scientists from the biotechnology sector to develop methods for deriving lines from single cells (termed blastomeres) that were removed from embryos that were grown for approximately 3 days in the laboratory. From the work of other scientists we knew that around this time the intrinsic genetic programs of the egg fade and those of the embryo start to unfold. Therefore, it was quite possible that human embryonic stem cell lines derived at this stage would be less specified than their counterparts, which were established by conventional methods. However, our initial work showed that this new approach was not very efficient. Although a few blastomeres formed lines, the majority did not. Therefore, we had to make this a more robust procedure. We took our clues for improving this method from the architecture of the embryo. Specifically, the cells are always grouped together and never found in isolation. Thus, to mimic this arrangement, we surrounded each blastomere with cells and proteins to simulate the geometry and molecular environment of the early embryo. The result was a much more efficient derivation procedure. Accordingly, we had developed the tools to perform the desired experiments.
For the derivation procedure, we used 5 embryos that were donated by a single couple. From single blastomeres removed at the 3-day stage we derived a total of 9 human embryonic stem cell lines. Thus, the lines were either genetically identical or related. First, we had to prove that they had the characteristics that researchers think are common to all human embryonic stem cells. Specifically, they expressed a panel of markers that are associated with this state. Also, in a laboratory dish, they made descendants of the 3 lineages that give rise to all the cell types in the body. The most rigorous test of the stem cell state is transplantation into a mouse where these cells are able to differentiate further. Like those derived by conventional means, our collection formed many structures such as bone, muscle and glands. Therefore, we concluded that they had the same general properties as human embryonic stem cells that were derived from later stage embryos.
Thus, we turned to the interesting question of whether they had unique properties. We characterized the cells at the genetic, epigenetic and functional level. At the level of genes, we compared the lines that were derived from a single cell of a 3-day old embryo to human embryonic stem cell lines that were derived by conventional means. There were striking differences in the gene expression patterns between the two. Epigenetic marks decorate DNA designating functional from nonfunctional areas in terms of gene expression. The lines that were derived from single cells had many fewer of these modifications suggesting less specialization. Finally, at a functional level, we showed that a subset of the lines we produced could spontaneously form cell types that are found in both the offspring and the placenta. Since these lineages diverge early in development, we took this as evidence of a greater degree of plasticity than is associated with the conventional lines. We think this property could be very useful for devising regenerative medicine therapies.
- Semin Reprod Med (2013) Human trophoblast progenitors: where do they reside? (PubMed: 23329637)
- Biol Reprod (2011) Trophoblast stem cells. (PubMed: 21106963)
- Differentiation (2011) GROalpha regulates human embryonic stem cell self-renewal or adoption of a neuronal fate. (PubMed: 21396766)
- Stem Cells Dev (2009) Derivation of human embryonic stem cell lines from biopsied blastomeres on human feeders with minimal exposure to xenomaterials. (PubMed: 19222349)


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1936 Ford Roadster - Driving a WinnerWritten by Eric Geisert on November 13, 2009 Steve Legens' '36 Ford roadster
Frequent readers of STREET RODDER will no doubt recognize Steve Legens' name. The owner of Legens Hot Rod Shop in Martin, Tennessee (started in 1991), Steve has had his cars-both customer's as well as personal-featured on the cover of STREET RODDER four times (a '48 Chevy truck in May 1999, a '36 three-window in April 2002, a highly customized '41 Willys truck in October 2006, and a '36 Ford coupe in November 2007) as well as having many other stories and articles published about the shop's ability to build a fine hot rod.
But if you look at the entire award-winning body of work the shop has produced, you might recognize a theme (besides all the trophies and accolades): A reoccurrence of the number 36-as in '36 Ford.
Though a Model A coupe was his first hot rod, a '36 Ford roadster has always been Steve's dream to own, but he has always found it difficult to hang onto any of his cars, as customers always seemed to want to buy them!
Back in 2005, while manning his booth at the NSRA's Street Rod Nationals in Louisville, Kentucky, one of his employees happened to mention to him there was someone who had a '36 Ford roadster for sale and was trying to get into the swap meet.
Now in a dead run from the building to the swap meet area, Steve found the owner of the car in the vendor parking area because he wasn't able to get the car into the swap meet. Steve checked the car out (it was in bare metal) and made an offer, but it wasn't accepted.
The next year Steve was driving back from Georgia after picking up a '38 Ford Standard and thought he'd call the owner of the '36 to see if he still had it. He did, so Steve drove over to check it out one more time. All of the sheetmetal was on the car, and it had wide whites mounted to black steel rims, but no motor. This time, though, he didn't leave without sealing a deal and two days later the car was at his shop.
Having built some high-end cars in his time, Steve's thought for this car went the other way. All he wanted to do was get a coat of primer on it, get a motor in it, then drive the wheels off of it. He found a Hemi motor and a five-speed for it, but the guys in the shop were sensing he wasn't going to keep it primered for long, and they believed the car would be getting coats of gloss sooner rather than later. So once the bodywork was done, Steve found what he thought was a pretty unusual color and he was off and running. Soon after he got hooked up with a set of Schott wheels, which he knew would look great under those round '36 fenders.
Unbeknownst to Steve, he was now on the side of a slippery slope of building more than just a driver and, as it started to be assembled, it kept getting nicer. But the guys in the shop doubted Steve when he said he was going to drive his roadster.
As it turned out the hot rod was getting finished near the time of the '08 Goodguys Nationals in Columbus, Ohio, and Steve thought it would be a good place to debut the car and enter it in their Street Rod of the Year contest. What he didn't know is that Goodguys had recently changed their guidelines on when a car needs to be on site (Friday at 10 a.m.) so at midnight on Thursday the Legens team was still working on the car and by 2 a.m. they had finished sewing the top and got the car in the trailer.
They entered the show (with a broken rear brake fitting, so only the front brakes worked) and the car was chosen along with four others as a finalist for Street Rod of the Year. Steve was honored to be picked, and though it didn't win the top prize, the car went on to win a Top 25 at the Shades of the Past event as well as being picked as a representative car at the SRMA booth at the SEMA Show later that year.
With some of the car shows out of the way, Steve was ready to really get in the car and do some driving. Mid last year he took the car on its maiden voyage as he drove three of his friends over the Ozark Mountains, putting roughly 2,000 miles on the odometer. That mileage total was soon improved upon with a trip to Texas for a Vintage Air kickoff party and then on to the L.A. Roadster Show in Southern California. The trip was made that much better as Steve's oldest son was able to go with him and ride shotgun (a present from him for Father's Day). They drove for 10 days with the top down all the way, even going through a few rainstorms. The 49-year-old says it was the most fun he'd had with a car since he'd finished his first hot rod way back in the late '80s, and he got to combine the best of both worlds as his '36 is both a driver and a winner.
|Martin, Tennessee
|1936 Ford Roadster
|Frame / Manufacturer
|'36 Ford / TCI Engineering (Ontario, CA)
|Chassis plumbing
|SS lines by Legens Hot Rods (LHR)
|Rearend / Ratio
|9" Ford / 3.00:1
|Rear suspension
|triangulated four-bar
|Rear brakes
|Wilwood (Camarillo, CA) disc
|Front suspension
|Front brakes
|Wilwood disc
|Master cylinder
|Kugel Komponents (La Habra, CA)
|power rack
|Front wheel make, size
|Schott Revolver (Los Alamitos, CA), 18x7
|Rear wheel make, size
|Schott Revolver, 20x10
|Front tire make, size
|BFGoodrich, 255/45R18
|Rear tire make, size
|BFGoodrich, 265/50R20
|Gas tank
|steel by Tanks (Clearwater, MI)
|Chrysler Hemi
|Machining / Assembly
|Cooling fan
|Walker (Memphis, TN)
|Valve covers
|custom steel and aluminum by LHR
|Stainless Works (Chagrin Falls, OH)
|Exhaust / Mufflers
|custom by LHR / Magnaflow (Rancho Santa Margarita, CA)
|Other engine facts
|custom air cleaner by LHR
|Tremec (Farmington Hills, MI) five-speed
|Clutch disc
|Pressure plate
|custom by LHR
|Wiles Welding
|Body style / Material
|roadster / steel
|Body manufacturer
|Body mods
|shaved door handles, cowl vent, hinges, rumble handle
|stock with hidden hood latch and handle by LHR
|by LHR
|Paint type / Color
|Spies Hecker / Rustrel Ochre
|by LHR
|stainless steel by LHR
|All American by Classic Instruments (Boyne City, MI)
|Stereo / Speakers
|Air conditioning
|Vintage Air (San Antonio, TX)
|Dynamat (Hamilton, OH)
|Ron Francis kit
|Steering wheel
|Billet Specialties Outlaw (La Grange, IL)
|Steering column
|Flaming River (Berea, OH) tilt
|custom by LHR with heated seats
|by LHR
|Material / Color
|leather / distressed sepia
|brown wool
|Juliano's (Vernon, CT)
|Other interior items
|custom console
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It's a simple interior, with a custom console added around the shifter. The steering wheel came from Billet Specialties while the column is a Flaming River unit. The stock dash houses a set of All American gauges from Classic Instruments.Steve set his steel-bodied '36 on a TCI Engineering chassis, which was equipped with an independent front suspension and a triangulated four-link out back. In the body modification department, the door handles were shaved, as was the cowl vent, door hinges, and rumble seat handle. Even the folding top, which Legens Hot Rod Shop covered, was lowered a little in the rear.Legens Hot Rod Shop created the bench seat before they tastefully covered it in distressed sepia-colored leather. The seatbelts are from Juliano's. The seat color nicely contrasts the dark brown carpet.A 6.1L Hemi was left alone internally while Legens dressed up the exterior with custom valve covers and polished pieces. Walker supplied the radiator, and the engine is backed to a Tremec five-speed transmission.Though it looks like a special one-off piece, these mini bumpers are sold over the counter at Legens Hot Rod Shop.


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Pashtun Pacifist
Khan Abdul Gaffar Khan, The Frontier Gandhi
1890 – 1988
Khan Abdul Gaffar Khan, proud Pashtun and devout Muslim from Charsadda in the Northwest Frontier Province of Pakistan, traveled the length and breadth of the subcontinent preaching the power of passive resistance and nonviolence.
Close friend of Gandhi's, his followers formed his non-violent army made up of Pashtuns, one of the most fierce warrior groups. The members of his army vowed to turn their backs on violence, revenge, and retaliation. They believed in the power of non-violence, education, and women's rights.
Jailed by the British numerous times, he said, "With love you can persuade a Pashtun to go to hell, but by force you can not take him even to heaven."
Nominated for a Nobel Peace Prize in 1985, his role in winning independence from British rule has been largely forgotten. His actions redefined the Pashtun concept of honor and courage.
Thousands of mourners attended his funeral, marching through the historic Khyber Pass from Peshawar to Jalalabad. A cease fire was announced in the Afghan war to allow safe passage to the funeral procession.
The world could use another giant of a man like Khan Abdul Gaffar Khan.


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Descriptionshine at your engagement party (and again at the rehearsal dinner) with our dazzling scatter necklace. it's crafted of crystal with a delicate dot motif, adding the perfect dose of sparkle to all the events surrounding your big day.
Shipping & Returns
- weight: 14g
- length: 16''
- lobster claw closure
- shiny 12-karat gold plated hardware
- faceted glass and rhodium
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I need to change my sight pin setup, its old and not very useful - especially for quick yardage adjustments. I have to estimate the yardage and use the same pin raising it over the target. I like to keep things simple, so glowing pins and dials etc... is avoided, plus I'm real cheap and don't like to spend money I don't have to.
Any of you out there have any suggestions?