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Reliable and warm, the Mountain Hardwear Pinole 20 Sleeping Bag makes the perfect companion for sleeping through chilly shoulder-season nights. Take this synthetic-insulated bag backpacking or camping when you're going out into the woods during dry or mild weather. Offset quilt construction eliminates cold spots, the nylon shell makes this sleeping bag highly packable, and the roomy footbox ensures comfort all the way down to your toes.
- Mummy shape allows you to move comfortably at the shoulders and tapers towards the footbox to maximize heat retention throughout
- Nylon shell is durable and highly packable and it features a water-repellent DWR coating for moisture and light precipitation
- Polyester lining feels soft and wicks moisture away from your skin so you feel comfortable while you sleep
- Thermic MX synthetic insulation is soft, lofty, lightweight, and easily compressed
- Footbox is shaped for natural foot position that allows for maximum warmth and comfort
- Face gasket and tailored hood comfortably block drafts so you retain more heat inside this sleeping bag
- Reviews
What do you think about this product?
Have questions about this product?
Sleeping Burrito.
- Familiarity: I've used it several times
If you want something that is light and comfortable, this is it. This sleeping bag is great because on the warm nights it doesn't feel like a sauna inside, but still keeps you nice and warm on the colder nights. I don't know how they do it, but it is awesome. Has the warmth of a mummy bag but you can still move around without having to roll over the entire bag when you want to move. The detail that they made it so it can attach to the ladies version of this bag is great and I haven't had any problems with this bag. The stuff sack is good quality and it makes it small enough to keep in a backpack.
Amazingly Breathable and Comfortable
- Familiarity: I've used it several times
One freezing cold night was all it took to know that I'd purchased a great bag for chilly desert outings, but a few weeks later, when the temps began to climb, and I still never got stuffy or sweaty in this bag - that's when I knew I made the right decision. I've slept in it down to around 20, and up to the low 60s, in total comfort. In fact, I hadn't slept as well in a tent for years until I slid into this bag. Great materials and construction, too. Love it.
Pinole is GREAT!
The Mountain Hardware PINOLE 20 degree Sleeping Bag is my favorite sleeping bag. The Mountain Hardware PINOLE 20 degree Sleeping Bag is decent on weight, 3.3 lbs, and provides great warmth down to 25 degrees. Packs up well. Cannot go wrong with the Mountain Hardware PINOLE 20 degree Sleeping Bag. Berz Rating 95/100


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Picaud, S., Strocchia, M., Terracciano, S., Lauro, G., Mendez, J., Daniels, D.L., Riccio, R., Bifulco, G., Bruno, I. and Filippakopoulos, P.
Notes: The authors used bioluminescence resonance energy transfer (BRET) to test the ability of a bromodomain 9 ligand to disrupt binding to histone. HEK 293 cells were cotransfected with a histone H3.3-HaloTag® fusion vector and either NanoLuc®-BRD9 bromodomain or NanoLuc®-full-length BRD4 fusion vector. After 24 hours, the transfected cells were trypsinized, diluted in phenol red-free DMEM with or without 10nM of HaloTag® NanoBRET™ 618 Ligand and dispensed into a 96-well plate. One of two potential BRD-disrupting compounds, 7d or 11, was adding to a final concentration of 0.005–33μM, cells were incubated for 18 hours and NanoBRET™ Nano-Glo® Substrate (final concentration 10µM) was added. Fluorescence was measured and a corrected BRET ratio calculated. Cytotoxicity was assessed after the NanoBRET™ assay by incubating the cells with the CellTiter-Glo® Reagent for 30 minutes and measuring luminescence. To examine histone H3.3 localization, HEK 293 cells were transfected with the histone H3.3-HaloTag® fusion vector using FuGENE® HD Transfection Reagent. After 24 hours, cells were labeled with 5μM HaloTag® TMR ligand for 15 minutes before washing with complete medium, incubated for 30 minutes and imaged with a confocal microscope. (4568)
Expand Full Notes »
NanoBRET™ Nano-Glo® Substrate
NanoBRET™ Nano-Glo® Detection System
HaloTag® NanoBRET™ 618 Ligand
FuGENE® HD Transfection Reagent
CellTiter-Glo® Luminescent Cell Viability Assay
HaloTag® TMR Ligand
Expand Products »
Clark, P.G.K., Vieira, L.C.C., Tallant, C., Fedorov, O., Singleton, D.C., Rogers, C.M., Monteiro, O.P., Bennett, J.M., Baronio, R., Müller, S., Daniels, D.L., Méndez, J., Knapp, S., Brennan, P.E. and Dixon, D.J.
Notes: To characterize the effectiveness of LP99, a potential bromodomain inhibitor, BRD7 and BRD9 were fused with NanoLuc® luciferase and histones H3.3 and H4 were fused with HaloTag® protein for use in BRET. The two proteins were expressed in HEK 293 cells, and the histone-HaloTag® fusions were fluorescently labeled with the HaloTag® NanoBRET™ 618 Ligand. Once the NanoBRET™ Nano-Glo® Substrate was added, NanoBRET™ ratios were assessed in the presence of varying concentrations of LP99. (4567)
Nath, N., Godat, B., Benink, H. and Urh, M.
Notes: This paper describes an improved method for on-bead conjugation of antibodies that overcomes the limitations of solution-based protocols (requirement for purified, high-concentration antibodies and the need for multiple buffer changes). The method uses HaloTag technology to immobilize protein A and G onto high-capacity magnetic beads. Antibodies are then captured and labeled on-bead. The authors demonstrate that the method is compatible with amine- and thiol-based chemistries as well as with mouse and human antibody isotypes, both purified and in cell media. Protein G and Protein A-HaloTag fusion proteins were constructed by cloning the coding sequences for the Fc-binding domains between N-terminal HQ and C-teminal HaloTag sequences. To create Protein A and G beads, the purified Protein G-HaloTag and Protein A-HaloTag constructs were covalently attached to Magne HaloTag Beads--magnetic cellulose beads activated with HaloTag ligands. (4586)
pHAb Amine Reactive Dye
pHAb Thiol Reactive Dye
Magne® Protein G Beads, 20% Slurry
Magne® Protein A Beads, 20% Slurry
pF1K T7 Flexi® Vector
Single Step (KRX) Competent Cells
Machleidt, T, Woodroofe, C.C., Schwinn, M.K., Méndez, J., Robers, M.B., Zimmerman, K., Otto, P., Daniels, D.L., Kirkland, T.A., and Wood, K.V.
Notes: This paper introduces NanoBRET technology, which provides an improved alternative to conventional BRET protein interaction assays. NanoBRET assays combine the extremely bright NanoLuc luciferase with a means for tagging intracellular proteins with a long-wavelength fluorophore (HaloTag). The greater light intensity and improved spectral resolution of the NanoBRET assay results in increased detection sensitivity and dynamic range over current BRET technologies. Performance of the assay is demonstrated using several model systems, and the ability to image BRET in individual cells is illustrated. The authors also demonstrate the application of NanoBRET in a novel assay developed for analyzing the interactions of bromodomain proteins with chromatin in living cells. (4575)
NanoBRET™ PPI MCS Starter System
NanoBRET™ PPI Flexi® Starter System
NanoBRET™ BRD4/Histone H3.3 Interaction Assay
NanoBRET™ BRD9/Histone H3.3 Interaction Assay
NanoBRET™ CBP/Histone H3.3 Interaction Assay
NanoBRET™ BRPF1/Histone H3.3 Interaction Assay
NanoBRET™ cMyc/MAX Interaction Assay
NanoBRET™ KRas/BRaf Interaction Assay
NanoBRET™ BRD4/Histone H4 Interaction Assay
NanoBRET™ BRD9/Histone H4 Interaction Assay
NanoBRET™ BRPF1/Histone H4 Interaction Assay
Ohana, R,F., Kirkland, T.A., Woodroofe, C.C., Levin, S., Uyeda, H.T., Otto, P., Hurst, R., Robers, M.B., Zimmerman, K., Encell, L.P., and Wood, K.V.
Notes: This paper describes a new method for capturing and characterizing the cellular targets of small molecules identified during phenotypic screening of compound libraries. The method uses a novel chloroalkane tag that has minimal effect on compound potency or cell permeability, allowing capture of protein targets in living cells. Interacting proteins are captured onto immobilized HaloTag® protein. The rapid, selective nature of this process enables capture of low-affinity or low-abundance targets. Exchanging the chloroalkane tag for a fluorophore, putative targets identified by mass spectrometry can be verified for direct binding to the compound using resonance energy transfer. The authors used the interaction between histone deacetylases (HDACs) and the inhibitor Vorinostat (SAHA) as a model system, and were able to identify and verify all the known HDAC targets of SAHA as well as two previously undescribed targets. (4578)
Wang, J., Ren, J., Wu, B., Feng, S., Cai, G., Tuluc, F., Peränen, J. and Guo, W.
Notes: To investigate whether protein conformation of Rabin8 plays a role in autoinhibition, the authors created a Rabin8 fusion construct with NanoLuc® luciferase at the N terminus and HaloTag® protein at the C terminus so that they can use BRET as an indication of protein conformation. A t-SRARE protein, syntaxin-4 (STX4), which is known to have a closed conformation, was constructed with the same NanoLuc® luciferase-STX4-HaloTag® protein configuration for use as a positive control. Both the control STX4 protein and Rabin8 were expressed in E. coli, the NanoBRET™ Nano-Glo® Substrate added and fluorescence measured. As a negative control, TEV protease was used to cleave the HaloTag® sequence from the protein fusions, eliminating the NanoBRET™ signal. NanoBRET™ signals were determined from experiments comparing Rabin8 with a gain-of-function Rabin8 mutant, exposing Rabin8 to constitutively active ERK2 or a kinase-dead ERK2 and assessing wildtype Rabin8 versus Rabin8-4D, where the aspartates acted as phosphorylation mimics. (4566)
Demont, E.H., Bamborough,P., Chung,C., Craggs, P.D., Fallon, D., Gordon, L.J., Grandi, P., Hobbs, C.I., Hussain, J., Jones, E.J., Le A., Michon, A., Mitchell, D.J., Prinjha, R.K., Roberts, A.D., Sheppard, R.J, and Watson, R.J.
Notes: In this paper the authors report on the discovery, binding mode, and structure:activity relationship of the first potent, selective series of inhibitors of the BRPF1 (bromodomain and PHD finger-containing) bromodomain. Bromodomains are specific protein modules present in a group of chromatin-regulator proteins responsible for "reading" acetylated lysine residues. Although some bromodomain-containing proteins (BCPs), such as those in the BET subfamily, are well characterized and have been identified as potential therapeutic targets, other BCPs, including those in the BPRF subfamily, are less well understood. These authors set out to generate selective inhibitors of the BRPF1 domain in order to better understand the functional role of this specific bromodomain region. Using an inhibitor discovery strategy based on other known compound-bromodomain interactions, a potent, selective inhibitor of the BRPF1 bromodomain was identified, synthesized, and characterized using in vitro methods. To demonstrate the function of this compound in live cells, the NanoBRET™ assay for protein:protein interactions (PPI) was used. The NanoBRET™ PPI assay enabled the authors to demonstrate both the cell permeability of the newly identified compound and also the ability of the compound to disrupt chromatin binding of the BRPF1 domain. NanoLuc® Luciferase-tagged BRPF1 bromodomain and HaloTag®-labeled Histone H3.3 were used for the NanoBRET™ assay in HEK293 cells. Dose-response curves performed with the NanoBRET™ assay enabled calculation of the cellular IC50 of the newly identified compound. A less active control analog compound was unable to inhibit the BRPF1 bromodomain:Histone H3.3 interaction, demonstrating assay specificity. Finally, the newly identified compound was inactive in NanoBRET™ assays using a second BRPF1 isoform containing a natural insertion, a result that was consistent with the proposed compound mode of action. Confirmation that the new identified compound can enter cells and disrupt the BRPF1 bromodomain:chromatin interaction in a cellular environment suggests that it may be a useful compound for studying the physiological role and therapeutic potential of BCPs containing the BRPF1 bromodomain. (4514)
Locatelli-Hoops, S., Sheen, Fangmin C., Zoubak, L., Gawrisch, K. and Yeliseev, A.A.
Notes: The HaloTag® Protein Tag was used to tag, immobilize and purify functional cannabinoid receptor CB2, a GPCR, after induction of expression in E. coli. (4263)
pFC20K HaloTag® T7 SP6 Flexi® Vector
pFN18K HaloTag® T7 Flexi® Vector
pFN19K HaloTag® T7 SP6 Flexi® Vector
Black, J.C., Manning, A.L., Van Rechem, C., Kim, J., Ladd, B., Cho, J., Pineda, C.M., Murphy, N., Daniels, D.L., Montagna, C., Lewis, P.W., Glass, K., Allis, C.D., Dyson, N.J., Getz, G. and Whetstine, J.R.
Notes: The HaloTag® protein tag was used in experiments to identify protein partners of KDM4A that involved in site-specific copy number gain in tumors, specifically at the 1q12h region. Expression constructs were transfected into HEK293T cells using the FuGENE® HD Transfection Reagent. The HaloTag-KDM4A (Cat.# FHC00602) and HaloTag-Suv39h1 (Cat.# FHC09879) expression constructs were obtained from the Kazusa DNA Research Institute (Kisarazu, Japan). Interacting proteins identified included DNA polymerase subunits and members of the minichromosome maintenance (MCM) complex. (4408)
pHTN HaloTag® CMV-neo Vector
HaloLink™ Resin
RQ1 RNase-Free DNase
Deplus, R., Delatte, B., Schwinn, M.K., Defrance, M., Méndez, J., Murphy, N., Dawson, M.A., Volkmar, M., Putmans, P., Calonne, E., Shih, A.H., Levine, R.L., Bernard, O., Mercher, T., Solary, E., Urh, M. Daniels, D. and Fuks, F.
Notes: These authors set out to determine how TET2 and TET3 proteins are involved in epigenetic regulation. To characterize proteins that interact with TET, the authors expressed full-length TET1, TET2 and TET3 as HaloTag® fusion proteins and performed protein pull-downs. They identified novel interactions between all three TET proteins and O-GlcNAc transferase (OGT), which catalyzes the addition of N-acetylglucosamine (GlcNAc) to numerous transcription factors, regulatory proteins and histones to activate or inhibit the target protein or recruit additional proteins. In this paper, they focused on TET2 and TET3, which showed the strongest interaction with OGT. They mapped TET2, TET3 and OGT binding throughout the genome by expressing these proteins as HaloTag® fusion proteins in HEK293T cells, crosslinking the proteins and DNA, then capturing the fusion proteins and associated DNA fragments and performing high-throughput sequencing to show that TET2/3 and OGT colocalize at active gene promoters and were tightly clustered near transcription start sites.
For expression of HaloTag® fusion proteins and controls, HEK-293 cells were plated at 12 ×106 cells in a 150mm dish and grown to 70–80% confluency before transfection with 30µg of plasmid using the FuGENE® HD Transfection Reagent.
To assess whether TET2/3-OGT activity affects the interaction of SET1/COMPASS with chromatin, the authors used bioluminescence resonance energy transfer (BRET). They created a fusion protein consisting of the H3K4 methyltransferase SETD1A and NanoLuc® luciferase as the energy donor and a fluorescently labeled histone H3.3-HaloTag® fusion protein as the energy acceptor. These BRET experiments confirmed that TET2/3-OGT activity is necessary for SET1/COMPASS complex function and showed that TET and OGT activities promote binding of SETD1A, a component of the SET1/COMPASS complex, to chromatin. This binding increases H3K4me3 levels. Thus, the authors' data support a TET2/3-OGT-mediated mechanism for regulating the SET1/COMPASS complex and thus H3K4me3. (4262)
HaloTag® Protein Purification System
Protease Inhibitor Cocktail, 50X
Galbraith, M., Allen, M., Bensard, C., Wang, X., Schwinn, M., Qin, B., Long, H., Daniels, D., Hahn, W., Dowell, R., and Espinosa, J.
Notes: These authors identified a previously unknown interaction between the transcription factor HIF1A and the cyclin-dependent kinase CDK8 (a component of the Mediator complex) in the regulation of genes associated with cellular survival under low-oxygen conditions. As part of the study, HaloTag technology was used to identify proteins interacting with CDK8 in a colorectal cancer cell line. Specifically, cells were transfected with CDK8 and CDK19 HaloTag fusion constructs obtained from Kazusa Institute. The cell lysates were then used in pulldown assays to capture interacting proteins. The results showed that CDK8 and CDK19 are present in mutually exclusive Mediator complexes. Details of the transfection are as follows: HCT116 cells were plated in 150 mm dishes and grown to 70%–80% confluence before transfection with 30 μg of plasmid DNA using FuGENE HD Transfection Reagent. (4355)
HaloTag® Mammalian Pull-Down System
Neunuebel, M.R., Mohammadi, S., Jarnik, M., and Machner, M.P.
Notes: In this study, the L. pneumophila protein Lem3 was expressed as a HaloTag® fusion protein and purified using HaloLink™ Resin. Lem3 was first cloned into the pFN22K HaloTag® Vector and the resultant HaloTag-Lem3 protein was expressed in Single-Step (KRX) competent cells before purification using the HaloTag® Protein Purification System. Lem3 was cleaved from the HaloLink™ Resin using TEV protease.
HaloTag® Protein Purification System Sample Pack
HaloTEV Protease
pFN22K HaloTag® CMVd1 Flexi® Vector
Urh, M., and Rosenberg, M.
Notes: This paper provides an overview of the many applications of HaloTag® Technology. The authors describe the development of the technology, focusing on it's multifunctional utility for protein imaging, protein isolation and display, and in the study of protein complexes and interactions. They also discuss it's potential to facilitate proteomics research studies across complex biological systems at the biochemical, cell-based and whole animal level. (4325)
HaloTag® Mammalian Protein Detection and Purification System
HaloTag® Mammalian Pull-Down and Labeling System
HaloTag® diAcFAM Ligand
HaloTag® Biotin Ligand
Yuanzheng, H., Xu, Y., Zhang, C., Gao, X., Dykema, K.J., martin, K.R., Ke, J., Hudson, E.A., Khoo, S.K., Resau, J.H., Alberts, A.S., Mackeigan, J.P., Furge, K.A. and Xu, H.E.
Notes: Yuangheng He and colleagues asked how the weak alkaline compound chloroquine (CQ) enhances the anti-inflammatory effects of synthetic glucocorticoids like dexamethasone, which are used to treat a host of inflammatory and autoimmune diseases. In the process they explored the intersection of lysosomal degradation pathways and glucocorticoid receptor signaling. They used Dual-Glo® Luciferase Assay System to look at glucocorticoid receptor-mediated (GR) activation and repression of reporters in AD293 cells under a variety of conditions (presence or absence of CQ; stripped serum, loss of lysosome synthesis, inhibition of V-ATPase, etc). They also used HaloTag® protein fusions to observe the fate of GR populations in the presence or absence of CQ and in the presence or absence of compounds that impair proteasome function. Live-cell imaging of GR-HaloTag® protein fusions revealed a dynamic association of the GR with lysosomes. The authors showed that glucocorticoid signaling is regulated by lysosomes. (4203)
Dual-Glo® Luciferase Assay System
Maruyama, A., Nishikawa, K., Kawatani, Y., Mimura, J., Hosoya, T., Harada, N., Yamamato, M. and Itoh, K.
Notes: These authors first used a FLAG-tagged protein (nfr2) with a HeLa Nuclear extract and captured interacting proteins via SDS-PAGE and in-gel digests of bands to identify (Krüppel-associated box)-associated protein 1 (KAP1) as a potential interacting partner. Human KAP1 was purchased as a HaloTag® CMV Flexi® Vector from Kazusa and used in a Mammalian PullDown scenario (with HaloLink™ Resin) to demonstrate interaction between the two proteins. A reporter assay was used to show that KAP1 facilitates Nrf2 transactivation in a dose-dependent manner. The authors defined the interaction sites using GST-tagged nrf2 and various forms of KAP1-HaloTag® Fusions expressed in TNT® SP6 High-Yield Wheat Germ Extract. GST-tagged proteins were expressed in E. coli and bound to glutathione-Sepharose beads. These bound proteins were mixed with the KAP1 from the cell-free expression system, incubated for 4 hours at 4°C, washed and stained with the HaloTag® TMR Ligand for 30 minutes. The proteins from the pull-down assay were subjected to SDS-PAGE and the HaloTag® proteins detected by phosphorimaging and the GST proteins by Coomassie Brilliant Blue Staining. A two-hybrid system consisting of the pRL-TK Vector with a firefly luciferase reporter with Gal4 UAS, mouse Nrf-2 N-terminal domain and KAP1 was also used. The vectors were transfected into Nrf2 knockout MEFs for 4 hours then incubated for 36 hours before luciferase expression was determined using the Dual-Luciferase® Reporter Assay System. (4123)
Anti-HaloTag® pAb
Dual-Luciferase® Reporter Assay System
pFN21A HaloTag® CMV Flexi® Vector
pGEM®-T Easy Vector System I
pGEM®-T Easy Vector System II
pGL3-Basic Vector
pRL-TK Vector
Neklesa, T.K., Tae, H.S., Schneekloth, A.R., Stulberg, M.J., Corson, T.W., Sundberg, T.B., Raina, K., Holley, S.A., and Crews, C.M.
Notes: These authors investigated whether HaloTag® technology could be used to specifically target and degrade intracellular proteins. They developed a method to degrade specific proteins of interest using a small molecule by tagging the HaloTag® protein with an adamantyl moiety. The hypothesis was that appending a hydrophobic residue to a protein surface domain would mimic partial denaturation and induce proteasomal degradation. They first used a HaloTag®-luciferase fusion protein to determine the biological activity of various small molecules that enhanced hydrophobicity. After selecting the small molecule that reduced luciferase activity in HEK293 cells, they went on to test their hypothesis in various in vitro and in vivo models. They were able to demonstrate degradation of various HaloTag®-linked transmembrane proteins expressed in HEK293 cells, to show degradations of target proteins in Zebrafish, and to show degradation of the HRAS oncogene product both in NIH3T3 cells and in a mouse tumor model. (4125)
Steady-Glo® Luciferase Assay System
Ohana, R.F., Hurst, R., Vidugiriene, J., Slater, M.R., Wood, K.V. and Urh, M.
Notes: The authors of this paper demonstrate the utility of the HaloTag® protein purification system for purifying functional proteins from mammalian cells. To this end five kinases were cloned into HaloTag® vectors, expressed in and purified from HEK293T cells. To demonstrate functionality of the purified recombinant kinases, activity was measured using the appropriate ADP-Glo™ Assay Kinase Enzyme Systems. (4145)
ADP-Glo™ Kinase Assay
ADP-Glo™ Kinase Assay + EGFR Kinase Enzyme System
ADP-Glo™ Kinase Assay + PKCγ Kinase Enzyme System
ADP-Glo™ Kinase Assay + SRC Kinase Enzyme System
Hattori, T., Müller, C., Gebhard, S., Bauer, E., Pausch, F., Schlund, B., Bösl, M.R., Hess, A., Surmann-Schmitt, C., von der Mark, H., de Crombrugghe, B. and von der Mark, K.
Notes: To study the role of the transcription factor Sox9 in the transition from cartilage to bone in newborn mice, the authors performed chromatin immunoprecipitation using the HaloCHIP™ System. Primary rib chondrocytes were transfected with a vector expressing full-length Sox9 with a HaloTag® protein tag, then proteins and DNA were cross-linked. DNA was isolated and sonicated, and the Sox9:DNA complexes were precipitated using the HaloLink™ Resin. The precipitated DNA then was amplified by PCR to determine that SOX9 binds to the SRY sites in the Vegfa gene. (4054)
HaloCHIP™ System
Kei Yamaguchi, Shinichi Inoue, Osama Ohara and Takahiro Nagase
Notes: The authors used the Halotag® Labeling Technology in pulse-chase experiments. They pulse labeled proteins in cultured mammalian cells. Using the HaloTag® Technology, they were able to monitor the degradation of the labeled protein, Smad1, that was induced by coexpression of Smurf1. They conclude that the HaloTag® Technology could be used to monitor the regulation of SMAD1 degradation. (4055)
HaloTag® Succinimidyl Ester (O4) Ligand
HaloTag® TMRDirect™ Ligand
Ohana, R.F., Encel, L.P., Zhao, K., Simpson, D., Slater, M.R., Urh, M., Wood, K.V.
Notes: The authors note that while many protein fusion tags are available to assist in purifying functional, recombinant proteins in soluble form and adequate amounts, none of the available tags are ideal when applied to the diversity of proteins studied. Available tags are often best-suited to specific aspects of the overall process, be it expression, solubilization, protein capture, etc. HaloTag was designed to address multiple features. The authors demonstrated that HaloTag provided enhanced expression and solubility in E. coli, with efficient protein purification and labeling for screening and quantitation. Enhanced solubility coupled with covalent capture gives HaloTag advantages over conventional affinity fusion tags, demonstrated by the successful purification of a wide variety of proteins, including low expressers, with higher yield and purity compared to the other tested tags. Covalent capture coupled with efficient tag removal to release the target protein, provides higher purity by eliminating tag contaminants. Using full-length cDNAs encoding 23 human proteins of varying size and function, the authors compared the efficacy of HaloTag as an expression and purification tag to the frequently used solubility enhancers MBP and GST. As reported previously, the three larger tags (HaloTag, MBP and GST) provided higher total and soluble expression compared to the smaller His6tag. In comparing soluble protein expression levels for the three larger tags, it was found that HaloTag solubilized 74% of the 23 human proteins examined, compared to 52% for MBP and 39% for GST. G2681 was one of the T7, bacterial promoter-based Flexi vectors used. (4005)
Schröder, J. Benink, H., Dyba, M. and Los, G.V.
Notes: Traditionally light microscopy resolution has been limited by the diffraction of light. However several new technologies have emerged that partially overcome that limitation. One of these stimulated emission depletion (STED) microscopy is now commercially available and has been integrated into confocal microscope platforms. Because STED depends on fluorescent markers that fulfill specific spectroscopic needs, its uses have been limited. The authors of this paper demonstrate successful high resolution of β1-integrin-Halotag®-fusion protein distribution using STED microscopy. Use of the HaloTag® technology allows researchers to create a reporter that can be labeled with STED-optimized fluorescent tags. (3955)
HaloTag® Alexa Fluor® 488 Ligand
Hurst, R., Hook, B., Slater, M.R., Hatrnett, J., Storts, D.R., and Nath, N.
Notes: These authors compared 6 different detection strategies for protein-protein interactions on protein arrays. They expressed HaloTag® labeled bait proteins in a cell-free expression system, and captured these bait proteins onto coated glass slides using the HaloLink™ Array System. They then compared detection strategies using prey proteins labeled as follows: 1)35S methionine, 2) fluorescence (BODIPY-FL) and 3) biotin labeling of lysine residues using modified Lys tRNA, 4) chemical labeling after expression, 5) HaloTag® fusion, and 6) N-terminal FLAG tag. The authors evaluated signal:background ratios, adaptability to high-throughput screening, and ease of use. (3999)
pF1A T7 Flexi® Vector
HaloLink™ Array (TnT® SP6 Wheat Germ) Two Slide System
TnT® SP6 Quick Coupled Transcription/Translation System, Trial Size
FluoroTect™ GreenLys in vitro Translation Labeling System
Transcend™ tRNA
Kardon, J.R., Reck-Peterson, S.L. and Vale, R.D.
Notes: These authors expressed recombinant dynactin and dynein in Saccharomyces cerevisiae and investigated their interactions in motility assays. They created a c-terminal Halotag-Dynactin fusion, and were able to site-specifically label the fusion protein with the fluorescent dye tetramethylrhodamine (TMR). They studied the effect of the purified dynactin fusion protein on the motility of dynein complexes using total internal reflection fluorescence microscopy. Dynactin alone did not interact with microtubules. However, when coincubated with recombinant dynein, the TMR-labeled dynactin moved processively along microtubules. The authors then used truncation mutants of dynactin to identify the region of the dynactin molecule required for localization and enhanced processivity of dynein. (3960)
Hasegawa, Y., Honda, A., Umezawa, K., Niino, Y., Oka, K., Chiba, T., Aiso, S., Tanimoto, A., Citterio, D., and Suzuki, K.
Notes: These authors describe a method for cell-surface labeling with two genetically expressed tags, a biotin acceptor peptide (BAP) and HaloTag (HT). BAP and HT were fused with the N-terminus of the mouse Ig κ-chain leader sequence to direct them to the secretory pathway, and the c-terminus of the platelet-derived growth factor receptor transmembrane domain to anchor them to the plasma membrane. Fluorescent proteins were fused with BAP and HT to create the two distinct fluorescent transmembrance proteins BAP-YFP-TM and HT-CFP-TM. The constructs were introduced into HeLa cells and imaged simultaneously. The authors then demonstrated in vivo imaging by injecting the doubly-tagged HeLa cells into nude mice and imaging with near infrared fluorescent probes, and with magnetic resonance probes. (3998)
Yamakawa, Hisashi
Notes: The authors use the Flexi® Cloning System to convert their cDNA clones to expression-ready clones. They wanted clones that could be used for comprehensive analysis with the HaloTag® Technology. They also describe a method of transferring ORFs between Flexi® Vectors in a 96-well plate format. They also used Wizard® SV 96 Plasmid DNA Purification, Wizard® SV PCR Clean-Up, and Wizard® SV Gel and PCR Clean-Up Systems. (4056)
Flexi® System, Entry/Transfer
Flexi® System, Transfer
Wizard® SV 96 PCR Clean-Up Start-Up Kit, 110V Electrical (8 x 96 preps, manifold and free vacuum pump)
Wizard® SV 96 PCR Clean-Up Start-Up Kit, 220V Electrical (8 x 96 preps, manifold and free vacuum pump)
Wizard® SV 96 PCR Clean-Up Start-Up Kit, 100V Electrical (8 x 96 preps, manifold and free vacuum pump)
Wizard® SV 96 PCR Clean-Up System
Wizard® SV 96 Plasmid DNA Purification System
Wizard® SV Gel and PCR Clean-Up Start-Up Kit, 110V Electrical (500 preps, manifold and free vacuum pump)
Wizard® SV Gel and PCR Clean-Up Start-Up Kit, 220V Electrical (500 preps, manifold and free vacuum pump)
Wizard® SV Gel and PCR Clean-Up Start-Up Kit, 100V Electrical (500 preps, manifold and free vacuum pump)
Wizard® SV Gel and PCR Clean-Up System
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text:
70147 Sir Fangar's Ice Fortress
Worriz and Gorzan, with his fire CHI power vehicle, must battle their way into Sir Fangar's Ice Fortress to reclaim the CHI. The Saber-tooth leader is forging weapons for the other awoken ice warriors in the weapons room, so they will need all of their skills to succeed. Move fast before the Saber-tooth warriors flip the bridge or activate the stairs trap. Evade shots from the ball shooter and avoid capture in the ice prison. Seek out Sir Fangar on his throne and grab the CHI! Includes 5 minifigures with assorted weapons: Sir Fangar, Strainor, Voom Voom, Worriz and Gorzan.


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Topic: G5 or Old Bonnie? (Read 2806 times)
Posts: 3
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G5 or Old Bonnie?
May 05, 2009, 04:17:44 pm »
Guys, Im looking for some advice. I can either outright buy a 71 Bonneville, good condition, for 4ish grand (still negotiating) which will wipe me out, or lay down the 2 grand for a new G5, and make payments. The plus side for the G5 of course is that its new, has almost limitless accessories at reasonable prices, and has a 2 yr. warrenty. The con side is that my Dad has always said not to buy anything its 1st yr. out. And im worried about the reliability issues since im not a mechanic. (Have read many scary Enfield stories) Also the break in time required.
The plus for the Bonnie is that I can afford it outright, and its already broken in of course. The cons are expensive accessories, very limited at that (has anyone ever seen a solo seat for a Bonneville?) and its a very old bike, which I like, but....
As far as shops to work on them, theres a Triumph dealership across town, and a Enfield dealership about an hour and 1/2 away. I really respect you guys opinions.
Thanks alot.
Grand Gearhead
Posts: 11,726
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World leaders in performance/racing Bullets
Re: G5 or Old Bonnie?
Reply #1 on:
May 05, 2009, 04:55:46 pm »
If you're "not a mechanic", you will be soon, with an old Triumph.
They are not "trouble-free" bikes.
But they are very nice.
You'll be doing wrenching on it. They have their known issues.
I'd say the G5 would be a far more reliable bike, but not as fast as the Bonnie.
The old Bonnies are great bikes. I love them, and they are real classic Brit-iron..
Just don't expect them to not need wrenching, because they will.
It's a tough choice.
My bet is the G5 will need less mechanic work.
C5 Pilot
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R.I.P Papa Juan, Uncle Ernie
Re: G5 or Old Bonnie?
Reply #2 on:
May 05, 2009, 05:01:24 pm »
Yep, Ace is right.
On the eighth day God created the C5, and it was better looking than anything on the planet.
Iron Butt Association
Grand Gearhead
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Re: G5 or Old Bonnie?
Reply #3 on:
May 05, 2009, 06:04:35 pm »
There will always be room in my garage for both a Trump and a Enfield or 3.
My Trump is kinda like my sport bike. I take it out maybe a few times a month.
My limit is about 100 miles on it and my hands and butt are numb. Maybe I should have left the motor alone eh?
I can ride my 99 Bullet for 12 hours straight and get up the next morning and do it again.
A real pleasure to ride.
To me the Enfield handles much better than the Trump. After riding the Bullet the Trump feels a bit heavy in the corners. 71's would be a bit heaver feeling than my 68 because the seat was higher because of the then new oil frame.
They fixed that in 73 I believe. 4K eh? 20 years ago you couldn't give a oil frame away. Funny how things change.
Every maintenance task is easier to do on the Enfield than it is on the Trump and both bikes are user interactive meaning you ride = you wrench. The better the wrench the better the ride.
My G5 is too new to tell but in 500 miles it hasn't had any issues yet. I bought it to be my "touring bike" for the next few years so I hope it stays that way. Either way I'm going to find out. (watch, I'll get grief for not riding a "real" Enfield to the REunions)
Maintenance and engine parts are easy to find for both the Enfields and the Trumps but if your looking for aftermarket accessories the Enfield wins hands down.
You got a tough decision to make pilgrim.
1999 Enfield 500 Black Deluxe "Silver"
2012 Concours 14 (no name yet)
2013 Royal Star Venture S "Jelly Roll"
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Re: G5 or Old Bonnie?
Reply #4 on:
May 05, 2009, 08:31:14 pm »
Wow, I knew I could get some great advice, thanks alot.
So now im thinking, if I could turn a wrench, I'd get the Bonnie, if I had more $$$ I'd go with the G5. So now my last question for you fine Gentlemen is, what is your opinion on late 70's, early 80's Hondas, Suzuki's? Im finding alot on Craigs for less than 3 grand? I appreciate everyones advice and i'll let ya'll know what I decide on.
bob bezin
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I ride therefore I am.
Re: G5 or Old Bonnie?
Reply #5 on:
May 05, 2009, 08:45:50 pm »
i have a 82 650 suzuki. the thing sat a while and no solvent could un gum the 4 carbs. so it sat some more . and because i had just put new tiers on it before this. i thought i have a shop clean and synk the carbs . something i could'nt do . well by this time the breaks were a little stuck and of course it needed a new battery. so i sunk $8oo. into a bike that would sell on craiglist for $1000. of course. it runs good .but with a triumph,norton, and endfield who wants to ride a suzuki? now i must ride it to keep it from gumming up again.
2000 RE classic , 56 matchless g80
2006 RE delux fireball 86 yamaha SRX 600
2015 indian chief vintage
65 500cctriumph
04 bonnie black
71 750 norton.
48 whizzer
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The world isn't beautiful, therefore it is.
Re: G5 or Old Bonnie?
Reply #6 on:
May 05, 2009, 09:23:59 pm »
I'd get the Enfield, hands down. Nothing beats having something new that you know is "yours." Now, if for that same 2k you got something like a Yamaha 650, well, that's another story.
All hail Sir Lucas, Prince of Darkness.
When an idiot thinks it's the same as not thinking at all!
Grand Gearhead
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Ride In Paradise Cabo, Don and Ernie
Re: G5 or Old Bonnie?
Reply #7 on:
May 06, 2009, 04:06:03 am »
I would like another 78 Suzuki GS 1000E. Parts and accessories can be problematic though. IF I could swing it I would get both. but that's just me.
If I were forced to choose one or the other I would get the Bullet first and continue to save and watch the classifieds for a <insert bike of choice here>.
I can break it better,,,,at night, in the rain, on the trail,, 20 miles from nowhere.
"TIMEX", the '06 Iron Barrel Military that takes me everywhere I want to go... and some places I shouldn't.
Blue Ridge Wheeltor
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Re: G5 or Old Bonnie?
Reply #8 on:
May 06, 2009, 04:55:35 am »
I have a '71 Bonneville. Beleive me, you'll want the Enfield.
2008 Royal Enfield Deluxe (Blue)
2006 Ural Patrol
1978 BMW R 100s--SOLD--
1971 Triumph Bonneville
Grand Gearhead
Posts: 3,613
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Looking for the next rebuild project....
Re: G5 or Old Bonnie?
Reply #9 on:
May 06, 2009, 06:01:18 am »
I'll ditto everyne elses feelings my first real bike was a basket case 71 750 Triumph. It was never a dull moment. You got good at repairing Prince of Darkness electrics in the middle of the night. Had to have aset of Whitworth tools (pretty expensive right now) and at times the patience of Mother Theresa. Cool bikes but probably far less reliable than the new Enfields.
As far as Japanese used it depends on how well they were maintained. Most cheap used are rode till they puke, rode some more and sold when they need major repairs. How about finding a used RE with low mileage? Decent cheaptransportation that won't break the bank. Check your local dealer first then Ebay for prices..you might be surprised. Will.
Will Morrison
2007 500 Military
2000 Kawasaki Drifter 1500
2000 Victory V92SC
1976 Suzuki GT185 Rebuilder Special..AKA (Junkyard Dog)
Many, many other toys.
The garage is full.
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Re: G5 or Old Bonnie?
Reply #10 on:
May 06, 2009, 07:45:21 am »
Not riden for over 40 years, I always wanted an old classic bike, in particular a Triumph, but then I had to decied if I wanted to ride or "tinker". After reading all the classic bike mags., and reading about Enfields, in particularly the new versions, my mind was changed. I wanted to ride.
I have a new Efi (DL) with only just 400 miles on the clock. I'm a bit slow at getting bit run in, but I'm nearly there, this is because I'm a fine weather rider, and I'm just waiting for the lousey weather to improve. I've had no problems with the bike as yet , but then its a new bike. With the Enfield Efi there is a minimum of maintenance to do, so its a case of cocking the leg and riding of into the sunset- eventually!
So ask yourself what do you want to do, ride or tinker.
Grand Gearhead
Posts: 11,726
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World leaders in performance/racing Bullets
Re: G5 or Old Bonnie?
Reply #11 on:
May 06, 2009, 08:02:54 am »
A good point was raised a few posts previous, about considering a low-mileage used Bullet. You can find them around $3k or less.
It will likely need some wrenching and/or fiddling, perhaps about what the Bonneville might need, but possibly less.
I bought a used 2000 Bullet, and after I repaired the initial broken part that the seller didn't know how to fix(which was why I got the good deal on it), it's been fine ever since.
Actually a 500 Bullet Iron Barrel model can be pretty reliable.
If you could fine one that does not have the Electric Start, you'd be ahead of the game. The Electric Start system is one of the major headaches on a Bullet, and the earlier models like mine didn't have any e-start.
Since I got mine sorted after I bought it, it's needed nothing. I've done some things to it, but not because they were broken. I just wanted to do some modifications for my own preference. The bike has actually needed no "repairs" since I got it on the road. It always starts, always rides, and has never left me stranded anywhere.
If you ask me, a used 500 Bullet can be a damn fine motorcycle. There may be some things you have to sort out that the previous owner screwed-up, or didn't know what to do. But once it's sorted, it's a good bike.
Last Edit: May 06, 2009, 08:05:47 am by ace.cafe
Posts: 154
Karma: 0
Re: G5 or Old Bonnie?
Reply #12 on:
May 06, 2009, 08:09:06 am »
Yes some good previous advice. I too have looked at the Bonnie option I was going to get a more recent example.
I guess the sensible option would probably to get a new UCE (if you can stretch to the intial extra cost)
I also agree with your Dad (as its how I used to feel) that you should give the new stuff (e.g. UCE) a year & let them identify & sort out some of the initial teething problems.
To counter that argument the new UCEs come with a 2 year warranty, Im fairly sure the dealers will be doing all they can to ensure the UCE customers are happy with their choice (you should get looked after & well supported). In Europe & the States the only new RE bikes that can now be sold are UCE(EFI). If these bikes fail & suffer a lot of faults there will be lots of bad stuff from unhappy owners all other the forums that aint going to do anything for RE dealers.
The good news so far is that the UCE (E5) bikes have been around for about 6 months (in UK) & there hasnt been any real issues (besides initial kick start probs) or gripes from people on any of the forums (the dealers seem very happy as well) the C5 has been around a couple of months now. I guess thats a good start for the new bike I guess the proof will be long term (you can get a fair few miles in on one over 2 years!)
Also remember that the UCE engine has been well tested & developed over the last few years.
Anyway from wanting an old iron RE to potter around on Ive really come round to the idea of the new C5.
Good luck with your choice anyway.
Grand Gearhead
Posts: 2,246
Karma: 0
Re: G5 or Old Bonnie?
Reply #13 on:
May 06, 2009, 08:44:18 am »
Initially you said you had $4K, but to buy an G5 you put 2 down.?? Put the 4 down and payments would be very low and you would have a very good machine. These bikes are manufactured using the newest technology, CNC. They've been tested for several years. I really don't expect any major problems with them.
I don't think the AVL, like I have, was tested as much and it's been 100% reliable, using almost the same new manufacturing technologies, for the last 10K + miles. To me it's a no brainer you won't regret it.
Last Edit: May 06, 2009, 08:46:24 am by PhilJ
Grand Gearhead
Posts: 2,567
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Yeah - I get it ....
Re: G5 or Old Bonnie?
Reply #14 on:
May 06, 2009, 04:26:30 pm »
Got a REAL nice old ('68) Turnip .... got an Enfield. Which one is held up by cobwebs and which one goes to work every day .... Hmmmmm! The Enfield would win, hands down, if I were making that decision. No Japper-Knees stuff either - not because they aren't DANDY bikes - but you just can't get many parts for the older ones beyond minor tuning stuff. I've had my share of those - and when they were about five years old and no one cared any more - HISTORY! Then I'd go out and find an old Brit to ease my pain ... I've kept a pile of them - but the Enfield get the nod for the daily driver.
Getting a gently used Enfield WOULD be a great compromise for you, I'd think. Then you could be one of US!! Hahahaha!!!
Spare the pig iron - spoil the part!
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Halloween Hunting at Birkdale Village
Birkdale Village hosted a Halloween Themed Scavenger hunt that was a HUGE hit with the kids and shoppers. Clue Sheets were available for participants to find items in the plaza's stores. Special Thanks to Birkdale for providing a great family friendly and safe event for our local families to participate in. Photos by Roger Benskin.
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Little Dragon's Ritual Union
Little Dragon is an electronic music group made up of four high school friends from Sweden. Their music is definitely modern, but you can hear influences of the past. The initial draw is definitely singer Yukimi Nagano's unique voice, but the music is the perfect match to her vocals. In this interview, Nagano describes her band mates as "sound nerds" and you can tell that they care about the final product.
I heard about them awhile back and borrowed Machine Dreams from the library, but for some reason it didn't grab me right away. When I heard their most recent album, Ritual Union, I was hooked. I loved a few songs instantly and then the rest of the album just kept growing on me. I worked my way back to Machine Dreams (which I now love) and also their self-titled debut album (which has some really great songs, but is not quite as solid as their follow-up albums).
Guest Appearances
Yukimi does guest vocals on a few albums that you can find at the library, including the Gorillaz Plastic Beach album, Raphael Saadiq's Stone Rollin album, DJ Shadow's The Less You Know, The Better album (great song and cool video), and the self-titled SBTRKT album. Her song "Wildfire" is definitely the highlight of the SBTRKT album. If you're really a super fan, you can dig even deeper and check out some of her earlier jazzy works, like "Summer Sun" from Koop's Waltz for Koop album. The Koop stuff is pretty different from her work with Little Dragon, though.
As evidenced by quite a few interviews on YouTube, they seem to be pretty down to earth and likeable people. There is a really great video (listed below) of them doing "Summertearz" with nothing but tuned bottles. They recently did some songs on the new Big Boi album (not out yet, but check out his great Sir Luscious Left Foot Son of Chico Dusty album and of course all of the Outkast). They have jokingly referred to themselves as a "no-guitar" band, but their sound is hardly lacking.
Little Dragon - Ritual Union
Little Dragon - Never
Little Dragon - My Step
Little Dragon - Twice
Little Dragon – Summertearz (with tuned bottles)
Little Dragon - Crystalfilm
Little Dragon - Runabout
SBTRKT – Wildfire
DJ Shadow – Scale It Back
Photo credit: Little Dragon
Author Bio:
Tommy M is excited to have discovered a new band that he likes so much.


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50/50 (2011)
50/50 SynopsisBased on an incredible real-life experience, 50/50 is a funny, touching and original story of friendship, love, and survival starring Joseph Gordon-Levitt, Seth Rogen, Anna Kendrick, Bryce Dallas Howard, and Anjelica Huston.
50/50 Release Date
In Theaters September 30, 2011
50/50 Credits
Starring: Joseph Gordon-Levitt, Seth Rogen, Anna Kendrick, Bryce Dallas Howard, Anjelica Huston
Written by: Will Reiser
Directed by: Jonathan Levine
Produced by: Evan Goldberg, Ben Karlin, Seth Rogen
MPAA Rating: [ R ]
Distributor: Summit Entertainment
External Info: 50/50 Official Website
Subscribe To 50/50 Updates
if you're excited for 50/50
The 10 Greatest Movie Potheads, Ranked
Things kind of got off to a bad foot between cinema and marijuana with Reefer Madness in the 1930s, but since then weve seen pot-addled characters make us laugh, solve mysteries, and go on all kinds of crazy adventures. Now theres a new stoner action-fest hitting theaters this weekend, so what better time to celebrate this grand history?
by Eric Eisenberg August 20, 2015 comments
The Writers Guild Nominates 50/50, Girl With The Dragon Tattoo And More For Top Awards
As usual, the Original Screenplay lineup is especially wide-ranging and interesting, with every film on the list qualifying as some kind of comedy despite being hugely different from each other. The category is also missing several ineligible powerhouse films, including The Artist
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The Top 10 Movies Of 2011: Mack's List
This list started as an unfilled Word document saying Best Movies on January 1st , and it ended with over one hundred films that have been obsessively tweaked. Theres no ulterior motives and no efforts to impress behind the choices Ive made. These are just the ones I liked a little bit more than the rest.
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The Top 10 Movies Of 2011: Eric's List
As both a film critic and reporter, I have to sit through a lot of terrible movies. This year I spent hours of my life watching movies like New Years Eve, Spy Kids: All The Time in the World, Abduction and Waiting For Forever. But its the good movies that make it all worth it, and this year there were some truly brilliant films in release. But among those great titles, what were my favorites? Well
by Eric Eisenberg December 30, 2011 comments
Josh's Top 10 Movies Of 2011
A decade spent working as a professional film critic has brought me here, to my tenth annual obligatory best of the year list. What you're getting here is a list of the 2011 movies which I think were the best. This list has the soul of a hero.
by Josh Tyler December 29, 2011 comments
8 Indies You Must See Before They Leave Theaters
But if you spent your Thanksgiving holiday seeing The Muppets, Arthur Christmas, Hugo and all the rest, you're either seeing Shame in limited release this weekend or totally stuck without options. Or are you?
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Photoshopped 50/50 Poster Says Absolutely Nothing About Cancer, The Movie
Marketing always was going to be the greatest challenge, the uphill battle, facing Jonathan Levines 50/50. Once the dramedy received the designation of being the cancer comedy, it didnt matter how many reviews sang the praises of the ensemble (virtually everyones fantastic) or the power of Will Reisers personal screenplay, all audiences would think about standing at the ticket counter was The Big C.
by Sean O'Connell October 18, 2011 comments
A Final Plea For 50/50: See It Because It'll Make You Stronger
Unlike any other kind of cancer movie youve ever sat through, youll walk out of 50/50 less afraid of the disease than you were when you came in. Not because the film makes it seem any less horrible than it is, but because 50/50 makes it clear that no matter how bad the cancer is, you can be stronger.
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Weekend Box Office: No One Wins When Hollywood Tanks This Badly
America took one look at the new offerings in movie theaters this weekend and passed, instead heading straight for the leftovers table. Despite four brand spanking new options hitting screens it was last weekend's top three who remained on top. After two weekends at number one, The Lion King traded places with last week's number three entry Dolphin Tale.
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50/50 Getting Warm Viewer Response
As anyone who follows Hollywood box office figures knows, the divide between critical acclaim and warm public reception is often very vast. Michael Bay has bought a lot of houses on the back of that conundrum, but heres why 50/50 is a fascinating case. Moviegoers actually seem to be loving it.
by Mack Rawden October 1, 2011 comments
What's Your Number? May Be Weekend's Biggest Failure
The early numbers are in and its turning into a bad weekend for nearly everything being released by Hollywood. Three new movies opened this weekend in wide release: Dream House, Whats Your Number?, and 50/50. While all three movies have received very different reactions from critics and audiences, all three are headed for instant failure at the box office.
by Josh Tyler October 1, 2011 comments
Great Debate: Does 50/50 Actually Have A Shot At Winning Oscars?
Now normally when you get a movie released in the fall about a main character who gets cancer, and when the critics falling all over themselves to praise it, you get oodles and oodles of Oscar buzz. But 50/50, a drama with heavy doses of comedy related to drugs, girls and other tawdry things that interest 20-something males, isn't exactly the kind of movie that automatically gets invited to the big awards show
by Katey Rich and Eric Eisenberg September 28, 2011 comments
This Rotten Week: Predicting 50/50, Dream House And What's Your Number Reviews
The Rotten Watch is coming off a stunningly terrible week and looking for a little rebound. It got ugly around here. Real depressing. Like crying naked in my bathtub, with a glass of Beaulieu Pinot Noir, listening to Damien Rice type of situation. Scary stuff. But this week weve got a bunch of stuff to cheer me up.
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Watch 8 New Clips From September Must-See 50/50
Come September 30th, if you're not in a theater getting ready to watch Jonathan Levine's 50/50 then you are doing yourself a serious disservice. Funny, touching, beautiful and filled with absolutely stellar performances, 50/50 is easily one of the best films of the year.
by Eric Eisenberg September 13, 2011 comments
Exclusive Interview: 50/50 Director Jonathan Levine
If youre not already excited to see Jonathan Levines 50/50, you need to remedy that problem right now. Though the film wont be released until September 30th, I was invited to go to a very early screening back in April and I was absolutely floored by it. Everything about it, from its blend of drama and humor to the performances by the entire cast, is stunning and last week I was given the chance to talk with the filmmaker on the phone about the upcoming release.
by Eric Eisenberg August 26, 2011 comments
We Can't Wait Until September: 7 Movies To Look Forward To Next Month
September, with its promise of cool weather and great movies, is just around the corner, and here at Cinema Blend we're already marking our calendars for the best of what's to come. We all picked one movie coming up in September that we're really looking forward to, whether they're festival hits we've already gotten to see, like Eric and Drive, or promising comedies that seem to make the most of our beloved stars, like Mack and What's Your Number
by CB Staff August 24, 2011 comments
New 50/50 Trailer Shaves Levitt And Showcases Anna Kendrick
Ive been told by those who have seen it that 50/50 should be my most anticipated movie for whats left of this year. Im pretty excited to see what Jonathan Levine is up to. He was the writer/director of a Sundance movie I loved called The Wackness and hes directing Joseph Gordon-Levitt, Seth Rogen, and Anna Kendrick in 50/50 as well.
by Josh Tyler August 11, 2011 comments
Joseph Gordon-Levitt Shaves His Head In Three New Stills From 50/50
Last week we posted the first trailer for 50/50 and I couldn't sing the movie's praises loud enough. It is easily the best film that I've seen thus far in 2011 and, though it's still early, it'll be a challenge for any other title the rest of the year to surpass it.
by Eric Eisenberg May 30, 2011 comments
Joseph Gordon-Levitt Has Cancer In The First Trailer For 50/50
Though the film isn't due out until September, a few weeks ago I was invited to a screening of the film 50/50, starring Joseph Gordon-Levitt, Seth Rogen, Anna Kendrick, Bryce Dallas Howard, and Anjelica Huston. After the credits began to roll, I was certain that I had just watched one of the best films that will be released in 2011.
by Eric Eisenberg May 27, 2011 comments
Seth Rogen's Cancer Comedy Retitled 50/50, Set For September 30 Release
It was hard to predict a release strategy for the film, given that it's a drama with comedic overtones-- always hard to market-- and stars Rogen and Gordon-Levitt, both of them successful in various ensemble films but not necessarily up to the task of carrying movies on their own. The Green Hornet's remarkable $200 million worldwide gross probably gave the studio a lot more faith in Rogen
by Katey Rich February 18, 2011 comments


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Those are some awesome pics. Never been there but definitely on my list. Thanks for sharing
twp1224, Thanks for sharing the pictures of Yosemite. I've never been there but its on my bucket list to go. Last year we took a family trip to northern CA and visited the redwoods along the coast. It was one of the prettiest places I've been. I couldn't believe the amount of elk along the coast up by crescent city.
Great pics, thanks for sharing! How difficult did you find the cables section of the hike? I have been looking into doing that one, but it will mostly likely not go off this summer for me.
I did the Fairy Loop, the Virgin, and Angel's Landing last year in a 3 day period in Bryce/Zion and while there were some difficult spots, I'd just consider those a good challenge and not super difficult.
To be honest, I'm not one for heights and the last ascend up those cables was tough. It's wasn't physically challenging, but mentally, for me, it was tough. There was a father taking his two young children, 5 and 7ish, up the cables when I was there. I figured if they could do it, I couldn't wuss out It was a long day. We started at sun up and didn't make it back to the truck until dark. If I were to do it again I would camp 2/3 the way there and start fresh in the morning. A lot of people were doing that. The views and terrain are awesome.
Great Pics !!


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New coffees, A new RSS feed
We added a new RSS feed to notify about new coffees. The link is feed://www.sweetmarias.com/rss/rss.php and it's easy to add to any browser (ie Firefox) or other device. Here's the new lots: Costa Rica Violeta -Don Teófilo Estate A clean, bright coffee with honey toast aromatics, bright lemon tea liveliness, hints of raspberry and strawberry, nutty roast tones, medium body, crisp aftertaste. El Salvador Los Luchadores Pacamara Tropical fruit aromas, lychee and passionfruit, with chocolate bittersweetness, baked peaches, mango, papaya, and anise seed. Kenya AA Auction Lot - Ndaroini A balanced Kenya with restrained acidity, grape, berry aroma and flavor with winey hints in the finish, syrupy sweetness, creamy body. Brazil Carmo de Minas - Fazenda do Serrado Lighter roasts have nut, caramel and orange rind hints with creamy body. FC+ roasts have chocolate, caramel, dense mouthfeel, minerals in the long finish. Great as SO espresso!


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Lab Assistants
All UMUC facilities are staffed by lab assistants during the posted hours. They can help you with general system problems, error codes, and matters of program syntax. If they cannot solve a problem, they will refer it to a member of the UNIX systems staff for further study. Lab assistants cannot interpret your assignments, debug your programs, or do your projects for you. These types of problems should be referred to your teacher or teaching assistant.


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Class AA-A: Catholic boys break through
CHARLESTON, W.Va. -- It took a little longer than Charleston Catholic would have liked, but the Irish ended up right where they wanted, and expected to be — on their way to another state championship game after a 2-1 victory against Bridgeport.
Catholic dominated possession for most of the first half of the Class AA-A boys soccer state semifinal at Friends of Coal Trace Fork Soccer Complex, but didn't score until senior Sam Revercomb curled a shot around Bridgeport goalkeeper Jonah James in the 32nd minute. That gave Catholic a lead it never reliquished.
The Irish (22-0-2) will play Fairmont Senior for its fourth state championship at 10 a.m. today.
That initial goal began with a free kick that first found the head of Dominic Cipollone before landing at Revercomb's feet. The senior took a touch and fired a shot that, according to him, he mishit. James dove to his left but could not get a hand on the ball and was left to watch it cross the goal line.
"We're always frustrated in our games because it always takes us a little while to get the first one," Revercomb said. "Usually once we get the first one we get the second and third but today they played well and we're just happy to get the win."
Charleston Catholic had plenty of opportunities, especially in the first half, but sometimes appeared to have trouble placing its shots on the goal. Irish head coach Sam Fox said taking better advantage of their opportunities was a point of emphasis at halftime.
"I wish we'd finished a few more than we had," Fox said. "I've told them all year that I think we're a better team than we've been playing. We played well today but I've seen these boys play since they were 4 and 5 years old, and I know these boys can play better than they have been.
"Don't get me wrong, I'm happy with how they played today but there's always room for improvement."
Charleston Catholic increased its lead in the 52nd minute and, like the first goal, it came on a set piece. Freshman Joey Trupo struck a corner kick into the penalty area that Revercomb was able to get his head on. A Bridgeport defender turned that shot away on the goal line, but the ball rebounded to the feet of Catholic's Tanner Whipkey who was able to poke it into the goal.
Bridgeport (15-5-2) and Charleston Catholic played during the regular season, with the Irish taking a 5-1 victory. Indians' senior defender Trevor Moon said defending Revercomb was a point of emphasis in practice, and that he thinks the team did a much better job of that Tuesday than they did in August.
"It's difficult, he's a very talented player," Moon said. "We did what we needed to do and did the best we could. For the most part we did very well containing him compared to the first game."
Bridgeport cut into Catholic's lead late in the game when senior Logan Lang beat Catholic goalkeeper Mitchel Thorstienson in the 77th minute to make the score 2-1.
But it was too little, too late for Bridgeport, as the Irish clamped down in the final minutes and punched their ticket to today's state final, with the chance to become the first boys team in state history to win four consecutive state championships.
It was fitting that on Election Day the Bridgeport girls soccer team applied the old cliche about voting "Chicago style" to their offense.
The Indians scored early and they scored often in their 7-0 win against Charleston Catholic in the second AA-A girls semifinal.
Bridgeport opened the scoring just three minutes into the game when freshman Mikayla Oliverio put the ball in the net, and Lauren McCarthy put Bridgeport ahead 2-0 in the ninth minute.
The Indians kept scoring, right up to Tori Coulter's tally in the 78th minute.
Catholic freshman Caroline Dundervill said that nerves were a factor for the young Irish.
"I think the freshmen were kind of scared being our first time out there," Dundervill said. "We really wanted to pull it off for the seniors."
In the other Class AA-A boys semifinal, Fairmont Senior easily dispatched Bluefield 5-1.
The Polar Bears struck first on a penalty kick by Justin Sloan in the 20th minute and added another goal two minutes later when senior Fletcher DeVaul was able to get a touch on a long free kick by Grant Holbert.
"The first goal is always a relief," Sloan said. "At the beginning we weren't exactly clicking but once we got that goal everything seemed to fall into place."
The Polar Bears (14-5-4) added a goal by junior Brent Pacoe in the 36th minute and another in the 43rd minute by senior Alec Wadsworth. Two minutes later, a corner kick found the head of Logan Demyon, who made it 5-0.
Bluefield (10-4-2) avoided the shutout several minutes later when Matthew Sabo beat Fairmont Senior goalkeeper Matthew Bartrug, but the Beavers could not get any closer.
Reach Tom Bragg at firstname.lastname@example.com


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Kansas City, MO (Sports Network) - The Kansas City Royals signed veteran
outfielder Endy Chavez to a minor league contract on Monday.
Chavez hit .203 with a pair of homers and 12 RBI in 64 games for the Orioles
in 2012.
In 974 career games with Kansas City, Montreal/Washington, Philadelphia, the
New York Mets, Seattle, Texas and Baltimore, Chavez has hit .269 with 26 home
runs, 229 RBI.
The Sports Network


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The skin around the eyes is extremely delicate. It is vulnerable to damage caused by aging, UV rays, fatigue, and hormonal changes. As a result, fine lines, known as crow's feet, and dark circles can form at the corners of the eyes. These symptoms can make an individual appear prematurely aged. CosMedix Eye Products combat these visible signs of aging. Potent plant extracts combine with powerful peptides and growth factors to strengthen the skin and stimulate the body's own collagen production processes. Used regularly, CosMedix Eye Products deliver measurable results, leaving the skin around the eyes firmer and more radiant.
By utilizing chiral technology similar to that in use in the pharmaceutical industry, CosMedix Eye Products deliver the benefits of biological ingredients direct to the delicate eye area. These chemically-pure skin care products allow high concentrations of active ingredients to be applied to the skin without irritation. At the cutting edge of cosmeceutical skin care technology, CosMedix Eye Products protect and nourish the skin around the eyes. The effects of aging are diminished, lines are reduced, and dark circles are lightened.
Incorporate CosMedix Eye Products in to your daily skin care routine and you will soon see visible improvement in the condition of your skin. These fantastic cosmeceutical products deliver high concentrations of active ingredients direct to the areas that need them most. The potent ingredients are well received due to their chirality, which enables them to work with the skin's cell structure. A highly effective delivery system allows beneficial molecules to penetrate the skin, to work in the lower layers of the epidermis. The production of collagen is stimulated, which strengthens the thin skin around the eye area. With increased elasticity, skin is better able to resist the development of fine lines and wrinkles. Eyes are brightened and appear more youthful.
Rejuvenate your eyes with CosMedix Opti Crystal. This delightful serum combines liquid crystals, alpha lipoic acid, and growth factors with natural extracts of coconut and beetroot. This unique formulation stimulates collagen production and strengthens the thin, fragile skin around the eyes. Each ingredient is chirally correct, ensuring that this product works in harmony with your skin structure to deliver visible results. The appearance of fine lines and wrinkles is diminished and dark circles are lightened. By gently patting a little of this potent serum around the orbital area each morning and evening, you will nourish, hydrate, and protect the skin. This serum can also be used on the skin around the lips, reducing the depth of the fine lines and wrinkles that can form around the mouth.


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In strangest Padres game of 2012, pitcher Richard unlikely offensive hero
Clayton Richard was hitless, but not hopeless. Overdue, but not overmatched.
The Padres pitcher was 0-for-16 with 10 strikeouts when he was pressed into service as a pinch-hitter Sunday afternoon and, as such, was an odds-on favorite to return to the dugout with his bat preserved in pristine condition.
He returned, instead, as the unlikely hero of the strangest game of the season — a 3-2, 13-inning, all-hands-on-deck affair that spanned four hours and 23 minutes, engaged all eligible position players and featured a rare quintuple shift by Los Angeles Angels manager Mike Scioscia. He returned as the shot-in-the-dark that struck the bull's-eye.
Richard started Sunday's decisive rally with a two-out single to left field and ended it by chugging around the bases when erstwhile second baseman Howard Kendrick bobbled Will Venable's subsequent single while attempting to impersonate a left fielder.
Welcome to baseball's Bizarro World, Petco Park edition.
"That thing that (Richard) did today, that's not what he does in (batting practice)," Padres third baseman Chase Headley said. "He just hits homers in BP. Bombs. I don't know where that (single) came from. I'm dead serious."
Even including Sunday's smash, Clayton Richard carries a career batting average of .116 and he had not scored a run since he trotted across the plate on an Adrian Gonzalez homer in 2010. But as both teams emptied their benches in search of a big blow, both managers were left to choose from lean alternatives.
His roster attrition accelerated by the loss of two left fielders to in-game injuries, Scioscia chose pitcher Dan Haren to lead off the Angels' 13th. Haren promptly struck out. Padres manager Bud Black settled on Richard by process of elimination. Scheduled to start Monday's game in St. Louis, Richard was ideally suited as a sacrificial offering to preserve the bullpen for additional innings.
To attach any additional insight or inspiration to the move was to confuse the random for the reasoned, to pretend this fluky chain of events could be imagined, much less foretold. Part of the charm of baseball games that go past 10 or 11 innings is watching managers try to make do with the pieces still at their disposal.
When Angels left fielder Ryan Langerhans injured his shoulder chasing an 11th-inning double by Padres pinch-hitter John Baker, the domino effect was a scorekeeper's nightmare.
Between batters, Scioscia dispatched Kendrick to left field, switched third baseman Maicer Izturis from third base to second, shifted first baseman Albert Pujols from first base to third, pulled catcher Bobby Wilson out from behind the plate to play first base, and inserted John Hester to catch.
After Venable bunted Baker to third base, recording the inning's first out, Kendrick changed gloves and returned to the infield. In an effort to cut off the winning run at the plate, Scioscia stationed five defenders in the infield and left left field vacant during Cameron Maybin's turn at bat.
Given Maybin's meager production, irregular contact and the Angels' oddball defensive alignment, this seemed like an excellent opportunity for a squeeze bunt and Black later acknowledged that he had considered that strategy. Instead, Maybin took three straight balls, fouled off the next three pitches, and then took a called third strike.
Scioscia looked like a genius. Maybin is looking more and more like another long-term commitment the Padres will live to regret. Still, since the Angels' reprieve proved only temporary, and the Padres moved past the Colorado Rockies into fourth place, the prevailing mood was mirthful.
"Almost all of the time as a starting pitcher, you have to sit and watch things happen," Richard said. "It was fun to be a part of it."


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We currently have hosted Mail Service from Rediff for our 5000 users. The management wants to move on to On-premise Zimbra setup. All users are on a single domain and currently access mails through web-mail or POP account configured on MS Outlook. We do NOT have iMAP access to our mails.
For migration, the number of users are too large, and I do not want to mess-up things on Split-Domain with a hosted server where we have very limited access, and sice we do not have iMAP access to the server, we cannot use imapsync. Please advice if the following approach should be fine for migrating mailboxes (and aliases and distribution lists) ONLY (no calendars / contacts / tasks):
For Web-mail users,
1> Install Zimbra Desktop in each of the end user's computer and configure POP account for existing mailbox on Rediff. Users continue to use Zimbra Desktop for their Rediff mail account for some time
2> Setup Zimbra Server and create all 5000 user accounts on the server
3> Communicate a go-live date to all users, on which we'll migrate the MX pointer to our on-premise setup. End users will need to create a NEW ACCOUNT on the Zimbra Desktop to connect to ZCS (just create the account, mails will still be seen on their Rediff Account)
4> On a friday, change the MX and A pointers to the Zimbra setup, so that all mails now land our Zimbra server. Company web-mail will also open up the Zimbra web-mail page (since the A pointer is changed)
5> Ask users to start using the new Zimbra account on the Zimbra Desktop, instead of using the Rediff Account
6> Using Zimbra Desktop, copy mails from Rediff Account Folders to New Zimbra Account Folders using drag-and-drop
7> Delete Rediff Account from Zimbra Desktop
For Outlook Users
1> On the date of go-live, create an account on their Outlook for ZCS
2> Continue to use Outlook as a client for the new ZCS for the moment
3> Send an IT Support Person to each of the Outlook users one-by-one, install Zimbra Desktop on their computer and migrate PST to Zimbra Desktop
4> Henceforth, use Zimbra Desktop instead of MS Outlook
I would need expert advice on this approach of migration, since it would be a large instantaneous migration for web-mail users that contribute to 70% (rest 30% use Outlook).
Also, can we migrate multiple PSTs to Zimbra? And what if there were some mails existing on ZCS Server, and we dump a PST on that account, will it overwrite Zimbra Mailbox? Or preserve mails on ZCS and just add PST to it?


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Alternative Dispute Resolution (3690)
This course is offered to students interested in acquiring knowledge of arbitration, mediation, conciliation, negotiation, client counseling and interviewing and other types of alternative dispute resolution systems and concepts distinct from traditional litigation. These systems and concepts are vital to a contemporary lawyer's practice.
Course Details
- Credits: 2, 3


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Stansport Apex Deluxe Arm Chair
|Eligible for Free Shipping on Orders $39 or More! (see promotion details)
|Members of The League get 5% back when you spend $100! (see promotion details)
Product Details
Kick back and relax in the Stansport® Apex deluxe arm chair, which is perfect for camping trips or tailgating fun. Adjustable arm rests feature a positive stop, cup holder and accessory bag, and the mesh back rest offers superior ventilation.
- Powder-coated steel frame
- UV-treated cross-woven polyester seat material
- Deluxe high padded back and arms for supreme comfort
- Mesh back rest for ventilation
- Adjustable arm rests with positive stop, cup holder and accessory bag
- Folds compactly and stores in polyester carry bag (included)
- Weight: 9.5 lbs
- Weight Capacity: 225 lbs
- Dimensions: W 22" x D 18.5" x H 35.5"
Product Recommendations
Product Reviews
Reviewed by 1 customer
Comments about Stansport Apex Deluxe Arm Chair:
Depth of the seat is listed as 18", but the seat back is sewn down 5" from the back so you only have 13" to sit on. Reaches to the middle of your thigh. Extraordinarily Uncomfortable!
Not worth buying.


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Seattle, WA (Sports Network) - Jordy Mercer's go-ahead RBI single in the top
of the ninth inning lifted the Pittsburgh Pirates to their sixth straight
victory, a 4-2 decision over the Seattle Mariners in the finale of a two-game
interleague set at Safeco Field.
With two runners on, Mercer ripped a two-out single back up the middle against
Yoervis Medina to put the Pirates in front. Travis Snider then scored an
insurance run when Medina's strikeout of Starling Marte went for a wild pitch.
Neil Walker had two hits and slugged a two-run homer for the Pirates, who
swept the West Coast part of the nine-game trip to finish the trek with a 7-2
record. They took all three from the Angels prior to their set with Seattle.
Vin Mazzaro (4-2) tossed a pair of scoreless innings and Mark Melancon worked
around two hits in the ninth to record his second save.
The Mariners have dropped five of their last seven contests. Charlie Furbush
(1-4) put the go-ahead run in scoring position and was hit with the loss.
The Sports Network


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You are here
Internship Statement Removal for Rules 46-47 or Prior Licenses Only
Submitted by Anonymous (not verified) on Fri, 09/23/2011 - 3:55pmPosted: Fri, 09/23/2011 - 3:55pm Updated: Tue, 01/06/2015 - 4:31pm
Rules 46-47 licenses and prior no longer have an internship statement required in the LVIS system. To view/print a copy of your license, go to the LVIS portal and create a profile. Once you are logged into your account, you may view/print your license by selecting the View/Print Licenses link on the right hand side under My Application.


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Worked With:
Kathy Griffin
Birth Name: Kathy Griffin
Born: 11/04/1960
Birth Place: Oak Park, Illinois, USA
Born: 11/04/1960
Birth Place: Oak Park, Illinois, USA
Born Kathleen Griffin on Nov. 4, 1960, Griffin was raised in the Chicago suburb of Oak Park. She was the youngest of five children of John, who managed an electronics store, and Maggie, a hospital administrator. Growing up, Griffin was taunted by schoolmates for her freckles and wiry red hair, but the sharp wit she developed as a defense proved to be the starting point of her career in comedy. Following her graduation from high school, Griffin spent a short period of time attending a local junior college before opting to move to California, where her parents wanted to retire and Griffin wanted to try to break into show business. One of her first jobs was as an extra in a 1984 Pepsi commercia... Continue Bio >>
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Body of missing oysterman found in Apalachicola
Published: Wednesday, July 24, 2013 at 9:54 a.m.
Last Modified: Wednesday, July 24, 2013 at 9:54 a.m.
APALACHICOLA — Authorities found the body of a missing 21-year-old oysterman about a mile from the spot his small boat sank in Apalachicola Bay.
The body was spotted Tuesday by crews in a Florida Fish and Wildlife Conservation Commission helicopter.
Brandon Wayne "Bruno" Creamer and 18-year-old Billie Murray were onboard the oyster vessel when it sank during a thunderstorm Monday morning. Murray was picked up minutes later by another boat.
The News Herald of Panama City (http://bit.ly/1bdIYq5 ) reports Creamer was clinging to oyster tongs for a while, but the current pulled him under.
Shrimp boats dragged the sea floor and divers searched for Creamer before his body was spotted by the helicopter crew.
Reader comments posted to this article may be published in our print edition. All rights reserved. This copyrighted material may not be re-published without permission. Links are encouraged.


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TMZ covers Rikishi
When Rikishi recently called out Kim Kardashian, a celebrity who is well known for his physical attributes, to compete in a big booty competition, the former WWE star was doing so, in hopes of generating media attention for his wrestling promotion. It looks like he has pulled that off rather successfully, as TMZ gave him some free coverage. The well known gossip website wrote an article about the challenge, and they even posted the video.
December 16, 2014


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Foxboro, MA (Sports Network) - The New England Patriots signed wide receiver
Michael Jenkins on Thursday. Terms of the deal were not disclosed.
A first-round draft pick of Atlanta in 2004, he spent the past two seasons
with Minnesota and 2012 saw him catch 40 passes for 449 yards and two
The Ohio State product has totaled 354 catches for 4,427 yards and 25
touchdowns over nine seasons in the NFL with Atlanta and Minnesota.
The Sports Network


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- Feliciano Lopez on cutting his lovely locks
Ah-ha! You see? Only good things can come from resolving a hairy situation even if it means growing it on your mug instead.
Lesson learned.
(Photo: Getty)
Labels: Feliciano Lopez, hair, player quotes, tennis
Change is always good. I say if he wants to actually start winning tournaments he should fix that backhand (read: play without a shirt.)ReplyDelete
Maybe Jelena and Ana can follow suit although they might need to shave their heads bald at the rate their Serbian slide is going.
Ps: The Serbian slide seems to be contagious, just look at Ana's beau Adam Scott who fell from No.3 to No.70+ in the rankings.
So anon above; you're saying the Serbian slide is...sexually transmitted, hmm.
Or maybe they're sharing needles. *gasp*ReplyDelete
Yeah the backhand is rough Sara but the skin is smooth. Yums.ReplyDelete
I'm not a fan of Feli's looks, he's not not a bad looking guy, he just doesn't move me. He should just shave the beard and grow the hair back. Now Nando....that's fine!!!ReplyDelete


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Video: Gym mat death: Scene pics point to foul play
Video: Two investigation into death of Valdosta teen
Video: New evidence may suggest Kendrick Johnson was murdered
VALDOSTA, Ga. -- When a doctor performed a second autopsy on 17-year-old Kendrick Johnson, he was shocked by what he found.
Dr. Bill Anderson determined the teen died from a blow to the neck, but he also made another discovery: some of Johnson's organs were missing. His lungs, heart and brain were not there, and the body was stuffed with newspaper.
The Georgia Bureau of Investigation claims Johnson's organs were placed back in his body after the first autopsy, but the Valdosta funeral home that enbalmed him said the organs were discarded before the body was sent to them.
RELATED | Second autopsy questions Johnson's death
RELATED | Groups demand answers in Johnson's death
For the local sheriff's department, Johnson's death is a closed book: A tragedy, but an accident.
State medical examiners concluded that Johnson suffocated in January after getting stuck in a rolled-up gym mat while reaching for a sneaker. That's a finding his family has never accepted, and one challenged by the findings of a second autopsy they commissioned.
Now, death scene imagery obtained exclusively by CNN has led a former FBI agent to question how the three-sport athlete died.
"I don't believe this was an accident. I think this young man met with foul play," said Harold Copus, now an Atlanta private investigator.
And Johnson's father, Kenneth Johnson, said he believes authorities aren't leveling with his family.
"They know something happened in that gym, and they don't want it to come out," he said.
A 15-minute video and nearly 700 photos taken by sheriff's investigators in Lowndes County documented the horrific scene. Johnson's body, clad in jeans and and layered orange and white T-shirts, was found wedged into a rolled-up wrestling mat in January. His face was bloated with pooled blood, some of which had poured out of his body, soaking his dreadlocks and spilling onto the floor.
There were more streaks of blood on a nearby wall -- but it wasn't Johnson's, according to investigators. Meanwhile, Copus said there appeared to be no blood on a sneaker that the teen supposedly was attempting to reach, located inches beneath him.
A pair of orange-and-black gym shoes found a few yards from the body had a substance that looked like blood on them, but investigators told CNN the stains weren't blood -- and so the shoes weren't collected as potential evidence. The same went for a hooded sweatshirt found a few feet away from the teen.
Copus said he can't explain how investigators handled items found around the gym.
"If you're running a crime scene, then you're going to say 'That's potential evidence. Obviously, we're going to check this out and find out who does it belong to,' " he said.
Lowndes County Sheriff Chris Prine refused to discuss the case with CNN, saying, "Our case is closed." But in June, an independent pathologist who conducted a second autopsy for the Johnson family found the teen suffered a blow to the right side of his neck that was "consistent with inflicted injury."
In May, sheriff's Lt. Stryde Jones told CNN that investigators tested the bloodstains on the nearby wall, "and it was not the blood of Kendrick Johnson." Investigators haven't determined whose blood it was, "but it doesn't appear to be involved in our crime in any way," he said.
"In the opinion of our crime scene personnel, after looking at it closely, the blood appeared as if it'd been there for an extended period of time. It didn't appear to be very fresh," Jones said.
But Copus said it's difficult to believe that old bloodstains weren't cleaned up.
"There is no way that they would allow whoever was supposed to clean this gym to leave that blood on that wall," he said.
In September, the Justice Department said it wouldn't open a civil rights investigation into Johnson's death. But federal prosecutors in south Georgia are reviewing the imagery to determine whether a separate investigation is necessary, the U.S. attorney's office in Macon told CNN.
For Johnson's father, the evidence is clear.
"Someone murdered him," he said. "They should be in jail."


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Moultrie Police say Jermaine Spradley turned himself in at about eight o'clock Monday night.
Investigators say 28-year-old Xavier Yates was shot after an argument. It happened at the corner of Third Avenue and Eighth Street Northwest on June 30.
"An officer was called to the emergency room. He had gone to the emergency room thinking he had gotten injured during the altercation that took place last week," says Moultrie Police Department's Corporal Dave Underwood.
Police issued warrants for the arrests of brothers Antonio and Jermaine Spradley who are now in police custody.
It happened late last week but Moultrie Police are still on the look-out for one more suspect, Jermaine Spradley.
Investigators say 28-year-old Xavier Yates, was shot after an argument. It happened at the corner of 3rd avenue and 8th street North West.
Investigators say one arrest has been made but anyone with tips can call anonymously and leave information at (229) 985-3131.
UPDATE 7-1-2011 AT 6:20PM by Caroline Gonzmart
Jerome Blakely lives down the street from the crime scene.
He says he's afraid to let his kids out of the house to play following the shooting because he never knows where gunfire could erupt next.
Says Blakely, "It's kinda hard to let your kids come outside and play ... somebody [could] just come out of nowhere and shoot."
The Moultrie Police Department says 28-year-old Xavier Yates was with a friend when they ran into brothers Antonio and Jermaine Spradley around 11:45 the morning of June 30, 2011.
MPD says after a disagreement, the Spradleys shot Yates in the head. They arrested Antonio Spradley and continue to hunt for his brother, Jermaine.
Blakely says his four-year-old daughter heard the noise of gunfire, but at the time he had no idea what it was.
He explains, "We heard a little 'pop,' and she asked me, 'What was that?' and I told her it was a firecracker."
But, the noise continued.
Explains Blakely, "About 15, 20 minutes later we heard another 'Pow, pow, pow,' and I told her, 'Well, now it sounds like they just lit a whole pack of firecrackers.' I found out it was gunshots."
The ground where the shooting happened still bears traces of victim Xavier Yates.
Blakely says if someone knows something, they should come forward -- and the Moultrie Police Department agrees.
Lead investigator Cpl. Dave Underwood says anyone with tips can call anonymously and leave information at (229) 985-3131.
UPDATE 7-1-2011 4:40pm
Moultire, GA - Police have arrested Antonio Spradley and are still seeking his brother Jermaine Spradley, who remains a suspect in the the shooting of Xavier Yates, 28.
Moultrie, Georgia - July 1, 2011 -
A Moultire man is in intensive care with a gunshot wound to the head. Police are searching for the suspects who pulled the trigger.
The incident happened around 11:45am on June 30th.
Investigators say Xavier Yates, 28, was shot after an argument.
It happened at the corner of 3rd avenue and 8th street North West in Moultrie.
Police have issued warrants for the arrests of brothers Antonio and Jermaine Spradley in connection with the shooting.
Police say they expect to make an arrest sometime today.
Stay with WCTV for details.


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ATLANTA -- About half the Georgians surveyed in a new statewide poll indicate that they would support public funding for a new retractable-roof football stadium. The new stadium would replace the Georgia Dome.
The survey of 500 adult Georgians conducted Wednesday night shows that 47 percent oppose using hotel-motel tax funds to help build a new stadium. 43 percent favor it. 10 percent are undecided.
When asked if they would support the project if it's "the only way to keep the Atlanta Falcons team playing in downtown Atlanta," 54% supported it and 37% opposed. The poll has a margin of error of plus or minus 4 and a half percent.
Previous polls have shown overwhelming opposition to the project. This is the first poll to ask specifically if respondents would support use of the hotel-motel tax, which is collected only from people paying for hotel rooms in metro Atlanta.
The billion-dollar stadium project would use $300 million in hotel-motel tax money. The remainder of the construction project would be funded by the Falcons, although the city of Atlanta would pay for expensive infrastructure improvements around the project. The city also collects a portion of the hotel-motel tax.
The stadium would be the property of the state, which would collect annual rent from the Falcons. The Falcons would reap concession revenue from all events at the new stadium.
Falcons officials have said that if the project isn't approved, the team will fund construction of an open-air stadium outside of downtown Atlanta.
The plan would need approval from the legislature. Current law allows the Georgia World Congress Center Authority to borrow up to $200 million from hotel-motel tax funds. The legislature would need to raise the cap to $300 million in order to complete the project.
Metro Atlanta respondents support the project slightly more than those outstate. Liberals and moderates support the project more than those identifying themselves as conservative.
To read the complete results, click here.


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Spring has sprung. When will winter end?
(Page 2 of 3)
In January 1821, the Hudson River froze solid, and people strolled between New Jersey and New York.
Many of us have suffered serious winter fatigue. One can admire the pristine mornings and dazzling, snow-laden trees for just so many weeks. We feel isolated and overweight. Cravings for carbs have driven our meal choices toward rice and pasta and bread and beans. Our skin is pasty, our muscles are atrophied.
Spring arrived last week, and the sun hovered above the equator, creating exactly 12 hours of sunlight and 12 hours of darkness. Spoiler alert: the Farmers' Almanac does not use the words "fair and dry" until its May forecast.
While we wait, let us turn to the poets and philosophers who offer their thoughts on the glories of spring.
April prepares her green traffic light and the world thinks Go. —Christopher Morley
Everything is blooming most recklessly; if it were voices instead of colors, there would be an unbelievable shrieking into the heart of the night. —Rainer Maria Rilke
It's spring fever. That is what the name of it is. And when you've got it, you want — oh, you don't quite know what it is you do want, but it just fairly makes your heart ache, you want it so! —Mark Twain
If we had no winter, the spring would not be so pleasant; if we did not sometimes taste of adversity, prosperity would not be so welcome. —Anne Bradstreet
April is a promise that May is bound to keep. —Hal Borland
Indoors or out, no one relaxes in March, that month of wind and taxes, the wind will presently disappear, the taxes last us all the year. —Ogden Nash
Every spring is the only spring — a perpetual astonishment. —Ellis Peters
Spring is sooner recognized by plants than by men. —Chinese proverb
The first day of spring is one thing, and the first spring day is another. The difference between them is sometimes as great as a month. —Henry Van Dyke
If you've never been thrilled to the very edges of your soul by a flower in spring bloom, maybe your soul has never been in bloom. —Terri Guillemet
Now every field is clothed with grass, and every tree with leaves; now the woods put forth their blossoms, and the year assumes its gay attire. —Virgil
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In collecting stories for today's Real Talk, I kept making this joke about how everybody's first time drinking was with screwdrivers, peach Schnapps or something really heinous like creme de menthe. But you know what? I was wrong. Everybody's first time drinking was fucking traumatizing. There's a few jolly drunk tales in here, but for the most part, the following gallery is full of 13 and 14-year-olds throwing up in painful and humiliating situations. Feel free to share equally horrible experiences in the comments.
Real Talk: What Happened The First Time You Got Drunk?
- 5 years ago by Ashley Cardiff
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