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After arriving from a week in Istanbul with all the carpet sales people constantly approaching us I was a bit skeptical when at check in, Mustafa sat down with us to discuss and plan our time in Goreme. He told us of the various tours, respected our budget, and shared pros and cons of each for me as ALL require... More
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subset: commoncrawl


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President Obama promised that the IRS would fully cooperate with Congress in its investigation into the IRS targeting of conservatives.
Now, in a bipartisan letter the Chairman and Ranking Member of the House Ways and Means Committee are complaining that the IRS is dragging it heels with Congress.
From Reuters:
Two lawmakers probing the U.S. Internal Revenue Service for its scrutiny of Tea Party and other conservative groups complained on Wednesday the IRS had handed over just a fraction of documents needed for their investigation.
Republican Dave Camp, chairman of the Ways and Means Committee in the U.S. House of Representatives, and Sander Levin, the senior Democrat on the committee, said they had received just 13,000 pages of the 65 million pages of information the IRS had identified as possibly relevant.
"We are concerned that, at this rate, the full production will take months," the lawmakers said in a letter to acting IRS Commissioner Daniel Werfel. They asked Werfel to explain how the IRS was sorting through the documents.
The IRS's response? The report continues, "The IRS said on Wednesday it had received the letter and planned to have more information available soon."
Just like their response the first time. Don't worry, it's coming. Just like don't worry, the targeting ended years ago or don't worry it was just some rogue agents in Cincinnati or don't worry senior IRS officials in let's say the Chief Counsel's office had nothing to do with this.
President Obama says the IRS targeting is just another one of those "phony scandals" that we should ignore.
Mr. President, if it's a phony scandal, prove it. Produce the documents. Lay out once and for all how the targeting began, continued for years, and forced conservative groups to turn over their donor lists.
The fact is the longer this drags out, the more evidence emerges that this scandal goes up, up, up the chain of command.
It's time for the truth.
If you agree, join the over 100,000 Americans in sign the petition to end the IRS abuse.
Today is traditionally known as Tax Day. (It was moved to Monday, April 18 this year to accommodate a federal holiday.) As millions of Americans work to beat the deadline to file tax returns, it's important to note that numerous legal challenges to hold a corrupt IRS accountable for targeting...
Today, the ACLJ presented oral arguments before the D.C. Circuit Court of Appeals in one of the largest lawsuits against the IRS for the targeting and discrimination against conservative non-profit organizations. Representing thirty-eight conservative grass roots organizations, the ACLJ opened its...
It's astonishing – truly mindboggling – a gross abuse of power. The Obama Administration is actually blaming conservative groups for complying with abusive and unconstitutional demands for information. To make matters worse, the Administration is threatening to publicly release reams of unlawfully...
It's one of the most stunning judicial opinions I've ever read. It is as clear in its scathing retort of the Obama Administration's IRS and DOJ as it is precise in its legal acumen. In what can only be described as a judicial takedown, the Sixth Circuit Court of Appeals unanimously delivers a...


text:
If you have any feedback or suggestions for our Editorial Team, please contact us at Editorial
Posted by Cbucks123 2 years ago
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The San Jose Sharks and the Pittsburgh Penguins will meet in the Stanley Cup Finals, and Game 1 goes Saturday. These are the two highest scoring teams in the playoffs, and because of that we see a higher than usual total (5.5). I expect both teams to have a more cautious and conservative approach in the series opener. Here are my keys to the game: 1. Previous History - Pittsburgh won the last meeting by a score of 5-1, but the total went under in each of the previous three meetings. Goals have been few and far between when these teams play in Pittsburgh, going under at a rate of 5-1-1 in the last seven. 2. Martin Jones - The Sharks netminder stopped 24-of-26 shots in a big win in Game 7 of the Western Conference Final, and he's 12-6 with a 2.12 GAA in the post-season. Jones has registered three shutouts in his last seven starts. 3. X-Factor - The under is 8-3-2 in Penguins last 13 Stanley Cup Finals games.Selection - This is a play on the Sharks@Pens to go UNDER the total (Free)
Copyright © 1995-


text:
France is one step closer to banning emails outside office hours
New legislation calls for companies to set their own email limits in a bid to reduce employee stress.
New legislation calls for companies to set their own email limits in a bid to reduce employee stress.
buying guideIn the market for a new set of noise-cancelling headphones? Don't take the plunge without checking out our favorites.
PC Gaming WeekOn its 20th anniversary, we take a look back at the Alienware's history and the gaming PC makers' innovations.
PC Gaming WeekIt's incredible how much the Total War rendition of Warhammer feels like the miniatures game – only more animated.
PC Gaming WeekBlizzard dishes big on its upcoming team-based shooter days ahead of launch.
Think you're up to speed with the Internet of Things? Using these recipes you can control your devices in ways you'd never dreamed of...
A recently uncovered Samsung patent suggests the next wave of smartwatches will put a few tricks up your sleeves.
Week in GamingSorry Popplio, but nobody likes you.
OpinionTesla's battery idea could put it on a highway to Hell.


text:
Issue 5 came out today. Click here to read.
Community Consultation Evening
The Consultation will include a number of guest speakers who will share their knowledge,
and give an overview of their input to the Health Curriculum at Wakari School. These will
include a representative from the Fire Service, Laurie Mahoney our Public Health Nurse and Constable Ross Greer.
Where: School Hall
When: Thursday 19 May at 4.30pm
Who: Any families interested
Why: This is an opportunity for the wider community to respond, and to give their input and ideas to the way Health Curriculum is taught at Wakari School.
For more information, click here.
Junior Syndicate Newsletter- term 2
What is happening in the Junior Syndicate this term? Click here for details.
Heritage Photo Orders
Heritage photo order forms will be going home with your eldest child this week. Please remember these points when ordering:
- Please use the correct change, either cash of cheque (NO EFTPOS) Cheques are to be out to "Heritage Productions Ltd"
- Deadline for orders is Friday 3 June
- Year 6 photo can be viewed in office
- The school receives 10% of total orders placed.
Thank you for keeping these points in mind.
Playball – term 2
Enrol now in Playball for term 2 at Wakari school on Wednesday's start date of 11th May in the school Hall 3-4pm 5-9 year olds only. For more info visit us on facebook, at our website www.playball.co.nz, email firstname.lastname@example.com or phone/text 027 554 8859.


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Artwork of Brij Mohan Anand adds a new chapter in Indian art h...Filmmaker-writer Imtiaz Ali, who inaugurated the May 12-22 exhibition, said BM Anand owned a great sense of dignity by laying uncompromising emphasis on the what he wanted to say rather than how Filmmaker-writer Imtiaz Ali while launching the ... Tehelka, 1 week ago
Committee to submit verfication report on seizure of cash of Rs 570 crore in TN
Salute to the unsung Maverick Illustrator Brij Mohan Anand Hiring centres to introduce high-tech machinery to farmers .... Shashi Kapoor made significant contribution to art cinema in the 70s & 80s: Biography .... Gendethur village shows ...United News of India, 1 week ago
Ghaziabad Authority to extend Indirapuram metro up to Mohan Nagar
Officials of the Ghaziabad Development Authority (GDA) on Wednesday said they have asked the Delhi Metro Rail Corporation to prepare estimates for a metro extension from Indirapuram to Mohan Nagar. The move is part of the authority's plans to ...Hindustan Times, 2 days ago
Anand Gajapathi Raju passes away
Scion of Vizianagaram royal family, former State Minister and MP Pusapati Anand Gajapathi Raju passed away here on Saturday. He was 65 and is survived by wife and a daughter. Union Civil Aviation Minster Ashok Gajapathi Raju is his younger brother. ...The Hindu, 2 months ago
Exhibition to throw light on remarkable works of rebel artist B.M. Anand
Communist Party of India New Delhi, May 11 Three decades after the passing away of a painter-illustrator whose independent works mirrored his fiercely anti-establishment spirit, Brij Mohan Anand is making a return of sorts to the art circuit with ...ProKerala.com, 2 weeks ago
More from: , Yahoo! India...and 1 other sources
Scramble for RS in Cong: Chidambaram may get wild card, Sharma to return
The deputy leader of the Congress in Rajya Sabha Anand Sharma has resigned from the Upper House of the Parliament. Nominated from Rajasthan, Sharmas term ended on 9 March. He would now be the Congress candidate for the lone seat from Himachal Pradesh, which ...catchnews.com, 2 months ago
Jungle raj: Stop targeting Bihar, Nitish Kumar, other states aren't models of mangal raj
So there is Jungle Raj in Bihar? Here is a tale from Ram Rajya, or rather Ram Raje in Rajasthan. Two days ago, a group of around 25 youth attacked with knives two students in the middle of a busy road in India's coaching hub Kota. One of them ...Firstpost.com, 1 week ago
THRC chief unhappy with dropout rate at school level
CPI(M) seeks EC to hold Aravakurichi Polls on May 17 or 18 .... Army officer injured in scuffle after jawan dies during training Thomas Pikety's international bestseller to become documentary film .... India's greying population to be ...United News of India, 1 week ago
MPs Flag Judicial Encroachment As RS Bids Farewell to 53 Member
The issues of alleged "encroachment" by judiciary on the rights of legislature and media accountability were today highlighted by MPs in Rajya Sabha as it bid an emotional farewell to 53 retiring members. Samajwadi party leader Ram Gopal Yadav ...Outlook India, 2 weeks ago
Merging ideology with art
12 May 2016, New Delhi, Team MP Art reflects one's ideologies and inclinations which might be otherwise inexpressible via any other form of communication. To showcase the best of the late artist Brij Mohan Anand, India International ...Millennium Post, 2 weeks ago
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Some Parisians want just a little bit more of their city to go to the dogs.
Some Parisians want just a little bit more of their city to go to the dogs.
At least 100 pooches – with owners in tow, holding leashes – marched near the Louvre at a demonstration to demand more park space and access to public transportation for the four-legged friends.
Tongues and tails wagged Saturday under a warm Paris sun as collies, pugs, boxers, bulldogs, Labradors and other breeds paraded around the famed Paris museum.
Organizers of the canine-citizen march dubbed "My Dog, My City" estimate about 200,000 dogs live in Paris. They say other cities like New York, London, Montreal and Brussels offer more dog-oriented public spaces.
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Most Read Stories
According to the city's website, two of Paris' 20 sections have only one reserved public park space for dogs. Leashes are required.


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Boston College suspends Troy Flutie after arrest (Update)
Flutie played in eight games at quarterback in 2015 and recently switched to wide receiver.Dr. Saturday
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Correction: Baylor-Sexual Assault-Timeline story
In a story May 24 about sexual assault cases involving Baylor University, The Associated Press misspelled the name of a Baylor spokeswoman on second reference. WACO, Texas (AP) - Baylor University, the largest Baptist school in the U.S., has been contending for the past year with cascading reports…The Associated Press
C-USA, ESPN agree to deal to air 6 football games
BRISTOL, Conn. (AP) -- Conference USA has agreed to a deal with ESPN to televise five regular-season football games and the league championship in 2016 and '17.The Associated Press
Reports: Under Armour and UCLA officially agree to largest apparel deal
The deal is reportedly worth $280 million over 15 years.Dr. Saturday
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Mark Richt donating $1 million to Miami's indoor facility
The Hurricanes recently announced plans to build an indoor practice facility. Richt went to Miami.Dr. Saturday
beIN Sports to broadcast 10 C-USA football games (Update)
C-USA's new television deal reportedly spans four networks, but its value is expected to significantly decline.Dr. Saturday
Oklahoma WR Dede Westbrook arrested for trespassing
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Reports: Michigan RB was run over by forklift last month
ANN ARBOR, Mich. (AP) -- Michigan running back Drake Johnson was run over by a forklift in a bizarre accident last month at the Wolverines' indoor track building, according to reports Tuesday.The Associated Press
Auburn defensive back Tim Irvin announces transfer
AUBURN, Ala. (AP) -- Auburn defensive back Tim Irvin is transferring to another school.The Associated Press
Duke brings in walk-on's parents for scholarship surprise (Video)
Duke DE Danny Doyle worked his way to the top of the team's depth chart and now he has a scholarship.Dr. Saturday
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Satellite camps, recruiting, hoops hot topics around the SEC
Southeastern Conference coaches and athletic directors will convene next week with familiar topics on the agenda, including football recruiting in general and satellite camps in particular, along with strengthening men's basketball across the league. The much-publicized issue of camps clearly isn't…The Associated Press
Former FCS standout Adams lands in CFL
Former Eastern Washington quarterback Vernon Adams Jr. hopes to do the same. Adams signed a three-year contract with the Montreal Alouettes over the weekend. The 5-foot-11, 200-pound Adams had a prolific career at Eastern Washington, finishing as the runner-up for FCS offensive player of the year…The Associated Press
Correction: Baylor-Sex Assault Investigations story
In a story May 24 about sexual assault cases involving Baylor University, The Associated Press misspelled the name of a Baylor spokeswoman on second reference. AUSTIN, Texas (AP) - Baylor University and its president faced mounting pressure Tuesday over how the school has handled reports of rape…The Associated Press
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Charges dropped against Georgia DL Jonathan Ledbetter
Ledbetter was arrested in March for underage possession of alcohol and false identification.Dr. Saturday
North Texas AD Rick Villarreal stepping down after 16 years
North Texas athletic director Rick Villarreal is stepping down after 16 years, a tenure marked by persistent instability in the football program despite the building of a new stadium. Villarreal said Monday the resignation was a ''mutual agreement'' with school President Neal Smatresk, who plans to…The Associated Press
Pac-12 agrees to limit late football games, court storming
The Pac-12 CEO Group has approved provisions to reduce night football games, add fines for court and field storming, and begin eSports competitions in 2016-17. The conference modified its TV agreements with Fox and ESPN to allow football games on the Pac-12 Networks to start at 2:30 p.m. or 6 p.m.…The Associated Press
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Social Hour: Frank Beamer gets a pedicure, mascot news and more
Miss something that happened on Dr. Saturday's social media presence? Well, we've got your roundup.Dr. Saturday
Forklift driver thought he hit starting block, not Drake Johnson
The former Michigan running back was hit by a forklift while stretching in April.Dr. Saturday
Pitt RB James Conner grateful as cancer goes into remission
James Conner's spring - much like that of former Pittsburgh teammate Tyler Boyd- included a phone call that changed his life. Months of worry and wearying cancer treatments that left the bruising running back exhausted at times ended for the Pitt running back on Monday, when Dr. Stanley Marks told…The Associated Press
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Auburn, Rutgers lose defensive backs to transfer
Auburn's Tim Irvin and Rutgers' Davon Jacobs will pursue other opportunitiesDr. Saturday


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Budget Moto X crosses FCC desk, packing familiar design
The Moto X was thought to be a budget-priced handset before it launched, but the mid-range specs come at the same $199 price we've come to know with most handsets. Now it looks like Motorola is preparing a cheaper version of the handset for the budget crowd, dubbed the DVX.
We know a few details about the Moto DVX, thanks to photos from a regulatory filing with the FCC. The DVX keeps a similar look to the Moto X, except it has swapped front-facing camera and sensors on the front and a swapped speaker grille and camera on the back.
The filing doesn't reveal if the DVX will include Moto Maker-like colours like the Moto X. It does say there is a GSM version (AT&T and T-Mobile) and a Sprint-compatible CDMA version, for the carrier's budget networks, in the plans. A leaked photo from budget carrier Republic Wireless slated the DVX for an October launch.
The Moto X hasn't made its way over to the UK as of yet. Motorola told Pocket-lint in August that it does have plans to launch a line of handsets outside of North and South America.
Does a budget version of the Moto X entice you to jump on board with the Motorola brand?
Best smartphones 2016: The best phones available to buy today
Samsung Galaxy S7 and Galaxy S7 edge: Release date, specs and everything you...
LG G5: Release date, price, specs and everything you need to know
Apple iPhone 7: What's the story so far?
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Robert Pattinson Denies 'Indiana Jones' and 'Star Wars' Casting Rumors
Robert Pattinson set the record straight on the rumors suggesting he's in talks for upcoming "Indiana Jones" reboot and "Star Wars" spin-off. The former "Twilight Saga" actor denies both casting reports while promoting his newest film "The Rover".
At a recent red-carpet event in Los Angeles, the British star said of speculation he could play the whip-wielding adventurer in "Indiana Jones V", "I don't know why. Why is that coming out?" He added, "I honestly don't understand what it's all about. Man, I wish!"
He was also clueless about a rumor saying he was eyed to play the younger version of Han Solo, a character made famous by "Indiana Jones" star Harrison Ford. "I didn't even know there was a Han Solo spin-off coming out," he said. "Sounds like a cool spin-off. I'll watch it."
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Sam Wilson is the New Captain America
Our New Favorite Director and Star Duo for Captain Marvel
The 10 Most Promising TV Shows of Fall 2015
Kaley Cuoco Shows Off Her Tennis Skills
6 TV Finales That Failed Spectacularly
Netflix is About to Spend $50 Million On Something Awesome
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15 Big Questions Left Unanswered by Star Wars: The Force Awakens
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Your Guide to the 20+ Superheroes and Villains Joining The CW-Verse This Fall
Civil War Featurette Explores Black Panther, Scarlet Witch and More!
Civil War Returns to Marvel Comics in 2015
Tom Hiddleston Reveals Why Loki Was Cut from Avengers: Age of Ultron
Will The Hulk Come Back?
Star Trek: New Series Could Run Many Seasons
The Walking Dead Executive Producer: What Actually Died in Sunday's Shocking Episode
Game Of Thrones Season 6 Delayed!


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Top Video Game Agent Leaves CAA for UTA
Ophir Lupu represents game developers and is the former co-head of CAA's video game department.
Ophir Lupu, the former co-head of CAA's video game department and an agent there, has joined UTA. He will head UTA's video games practice.
At CAA, Lupu represented video game designers and creators such as Ken Levine (BioShock), Patrice Desilets (Assassin's Creed) and Cliff Bleszinski (Gears of War). He also represented gaming companies Valhalla Studios (Devil's Third), Crytek (Homefront 2) and Klei Entertainment (Shank).
In January, Lupu orchestrated the acquisition of indie video game studio Area/Code by Zynga, the creator of hits FarmVille and CityVille.
"Ophir's accomplishments are tremendous, and his vision of where the games business is going makes this a very exciting opportunity for UTA," said Jeremy Zimmer, UTA co-founder and managing director in a statement. "We believe he and his clients will thrive in our collaborative culture."
UTA's video game department represents game developers, publishers such as Electronic Arts, and technology companies including Nyko and Playcast.
Email: firstname.lastname@example.com
Twitter: @DanielNMiller
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- Dwayne Johnson Shares First Look At Hobbs In 'Fast 8' And It's All Kinds Of Intense
- John Carpenter Has Some Choice Words About Friday The 13th, Specifically, 'It Just Doesn't Rise Above Its Cheapness'
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- How Mindy Kaling's Subtle, Cunning Lies Affected B.J. Novak's Psyche While Filming The Office
- Italian Star of Stage and Screen Giorgio Albertazzi Dead at 92


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Gifts for the World Traveler
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Community colleges as relevant to all.
May 27, 2016
John Fea, a history chair, describes what he learned on his daughter's college tours about the presence or absence of a liberal arts ethos.
May 26, 2016
Timothy V. Kaufman-Osborn takes issue with arguments that the erosion of faculty members' participation in shared governance is entirely due to forces beyond their control.
Recent Articles
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Let's hope they take good care of it.
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Learning from dead malls and big malls.
October 23, 2007
Everyone knows that rock and roll is all about kicking out the jams: ditching uptight squares, taking long rides in the dark of night, and being a street fightin' man -- or woman. As The Who put it, it's about hoping to die before you get old. But what does rock mean to a new generation of uptight (if updated and wired) squares, afraid of the open road, who have little fight in them? What does rock mean for a generation that has never been allowed to be young -- let alone hope to die before they get old?
October 22, 2007
Discussions of how to diversify the research world and of how to work beyond the boundaries of traditional fields should be combined, write Diana Rhoten and Stephanie Pfirman.
October 19, 2007
Carolyn Segal considers the impact of an essay -- and its unexpected afterlife in the world of plagiarism Web sites.
October 18, 2007
Stephen Joel Trachtenberg, a president-turned-presidential search consultant, considers why many candidacies are needlessly ruled out, and suggests that the questions asked may be too narrow.
October 17, 2007
They long for a clash of civilizations that would destroy our liberties. Scott McLemee worries about the fanatics among us.
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The top 10 players most likely to be traded
Leave it to the Ducks and Toronto Maple Leafs to jump start the trade market with a deal last week that presumably will help both sides.
Both teams are far from done, and there figures to be plenty of other movement with the Feb.28 deadline looming as teams are tightly packed in the playoff race.
There are only a handful of teams that are truly out of contention, specifically Toronto, Ottawa, Edmonton and New Jersey, which means they are the identified sellers.
Toronto general manager Brian Burke and Ottawa GM Bryan Murray have let it be known that they are not done dealing, while the Ducks need to cut loose some extra defensemen.
Take a look here as the Register brings you the top 10 players most likely to be traded at the deadline.
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Join Date: May 2009
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Hey ladies!
My husband is going to be stationed at Campbell, and we are coming at the 30th of this month. We want to live in the country, cheap, and be able to have animals and garden. It's always hard for us to find a place since we live as green and off the grid as possible! Does anyone know of an area that is less than an hour from Ft. Campbell where we could look into? Thanks in advance!
Kourtney, happily married to my soldier and raising ds 7/08 .... dd 7/10..... and ds 11/11
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text:
Let's take a look at some money idioms, starting with 'lucky penny'. The OED describes a 'lucky penny' as usually one that is bent or perforated, or sometimes an old or foreign coin. In the early nineteenth century, a 'luck-penny' was defined as 'the cash which the seller gives back to the buyer after the latter […]
Glasses, shenanigans, and other words that are always plurals. (Also: cats!) oxford.ly/1PtoHkz pic.twitter.com/zNp7zOu6vx
Word of the Day: diachronic - concerned with how something develops through time... ... ow.ly/eYgy500LrR0
You can now vote for your favourite language learning blog @babla: bit.ly/20FmLXi
The Great British Debate: how do YOU pronounce 'scone'? oxford.ly/1gJCLDh pic.twitter.com/3ecSlUuLdn
Word of the Day: tweeps - a person's followers on Twitter…... ow.ly/mVjR500J81j


text:
My recording of my snare hit with a higher tone.
Same as 14 but not as much tail.
Male vocal shout 'hah!'
Sound produced when hitting the ball on the floor. Recorded by a Zoom H2
ball tapping catch pass basketball
Basketball bounce, recorded in a small shed, Zoom H2
Reading from a piece made up entirely of questions composed on an autumn morning, by Joaquin Flipp from his book ...
This was a quote from a gogo dancer asking a question, but got mixed up halfway the sentence, making it ...
4chan reference in Russian accent
The question, "Are you experiencing severe mental anguish?"
My new friend Erico ( https://soundcloud.com/erico-wakamatsu ) is helping me with a project I've been working on & I asked ...
"I am Takahashi Hanako. Let me know if you want to change your look!" a request from Mearii23 (sorry it's ...
Japanese introduction for a request...with an American accent =P


text:
Foo Fighters Release Humorous Coloring Book Tour Rider
The rider was made by Gus Brandt, the Foo Fighters' tour manager. A section of the rider entitled Catering Visual Enhancement & Activities depicts the differences between a lame entree and an awesome entree, the differences between a good salad and a bad salad, what constitutes a great breakfast, a guide to making good ice cubes, and more. The document also includes a word hunt, among other fun games and visual comparisons.
The guidelines in that section include suggestions like "colored pencils work best," "remember to stay outside of the lines," and "originality, creativity, and pure sarcasm are what we're looking for." Most importantly, "don't cheat on the maze."
"Most of the rider is meant to make sure that the road crew are fed properly," Grohl said. "To be honest, that rider has more to do with them than anybody else. I could survive on microwaved burritos and Crown Royal." What a man.
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Judd Bruce "Slow Joe" Doyle (September 15, 1881 in Clay Center, Kansas – November 21, 1947 in Tannersville, New York) was a right-handed baseball pitcher who played from 1906 to 1910 for the New York Highlanders and Cincinnati Reds. He thre...w a sinker, a "raise ball" and a spitter ([1]).
Connect any celebrity with Slow Joe Doyle to see how closely they are linked... romantically!


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Where are the SATA 300 Cables?
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All I can say is,
#1 They are for REAL, unlike some other 'Ellusions.'
#2 - they are not 'BIRDING' around like the penguins. #3 BEST customer...
Read More ->
I have been buying from MJM for a very long time.
Their merchandise is excellent. This close to Christmas
I got my order in the time period they...
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MJM excellent service, buy best your money and fast shipped like MAGIC *~*~*~*~
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FASTERRRR........!!!! again and again, trust me and you will get surprise :))
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I "Must" thank everyone at MJM Magic for the "Outstanding" Customer Service they provide. I have had several orders placed with...
Read More ->


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Posted: 9/15/2015
Called at 5:56 p.m. and the telephone was answered by a staff member. I asked if they accepted my insurance and she said they did. I wanted to make an appointment and was told to call back in the morning as they were getting ready to walk out the door. Not great customer service. Will look elsewhere.


text:
Bring a buddy.4.55 / 5.00 9,767 Views
Yugi and Kaiba have a duel to the death!4.48 / 5.00 35,987 Views
Vampire Pixel in the Neon City
A sinister deal is underway in the underbelly of Neon City4.34 / 5.00 11,219 Views
Errr? Lol?
Okay, well, first of all, it's too bad more people didn't join this collab, I think it would have been better if they did.
Though, it was kind of funny and pritty random ;D
The sound on yours though iShrim, needed alittle more work maybe?
Could have even had some quiet background music in this ;)
Overall though, this was a good collab even though it was alittle short.
And look at that, I was a part of it :O
Good job.
yeah if more people joined then it would be more better and longer
Kinda, random.
Well, it doesn't really have a storyline. It was a bit confusing.
Like the guy before me sayed.
Could use some attention at the graphics and the sound.
The cursor was funny to play with though...


text:
Enhancement of the thermostability and the catalytic efficiency of Bacillus pumilus CBS protease by site-directed mutagenesis
The serine alkaline protease, SAPB, from Bacillus pumilus CBS is characterized by its high thermoactivity, pH stability and high catalytic efficiency (k(cat)/K(m)) as well as its excellent stability and compatibility with an alkaline environment under harsh washing conditions. Based on sequence alignments and homology-modeling studies, the present study identified five amino acids Leu31, Thr33, Asn99, Phe159 and Gly182 being putatively important for the enzymatic behaviour of SAPB. To corroborate the role of these residues, 12 mutants were constructed by site-directed mutagenesis and then purified and characterized. The findings demonstrate that the single mutants F159T, F159S and G182S and combined double substitutions were implicated in the decrease of the optimum pH and temperature to 8.0-9.0 and 50 degrees C, respectively, and that mutant F159T/S clearly affected substrate affinity and catalytic efficiency. With regards to the single L31I, T33S and N99Y and combined double and triple mutations, the N99Y mutation strongly improved the half-life times at 50 degrees C and 60 degrees C to 660 and 295 min from of 220 and 80 min for the wild-type enzyme, respectively. More interestingly, this mutation also shifted the optimum temperature from 65 degrees C to 75 degrees C and caused a prominent 31-fold increase in k(cat)/K(m) with N-succinyl-l-Ala-Ala-Pro-Phe-p-nitroanilide (AAPF). The L31I and T33S mutants were observed to improve mainly the optimum pH from 11.0 to 11.5 and from 11.0 to 12.0, respectively. Kinetic studies of double and triple mutants showed that the cumulative effect of polar uncharged substitutions had a synergistic effect on the P1 position preference using synthetic peptide substrates, which confirms the implication of these amino acids in substrate recognition and catalytic efficiency.
- CitationsCitations34
- ReferencesReferences52
- "One keratin azure unit was defined as the amount of enzyme causing an increase of 0.01 in absorbance at 595 nm in one min under the experimental conditions described. Keratinolytic activity was also measured using the Folin-Ciocalteu method and as previously described elsewhere [31] with keratin as a substrate. One keratin unit was defined as the amount of enzyme that hydrolyzed the substrate and that produced 1 µg of amino acid equivalent to tyrosine per min at 50 °C and pH 7 in buffer A. "
Novel serine keratinase from Caldicoprobacter algeriensis exhibiting outstanding hide dehairing abilities[Show abstract] [Hide abstract] ABSTRACT: The current paper reports on the purification of an extracellular thermostable keratinase (KERCA) produced from C. algeriensis strain TH7C1T, a thermophilic, anaerobic bacterium isolated from a hydrothermal hot spring in Algeria. The maximum keratinase activity recorded after 24-h of incubation at 50 °C was 21000 U/ml. The enzyme was purified by ammonium sulfate precipitation-dialysis and heat treatment (2 h at 50 °C) followed by UNO Q-6 FPLC anion exchange chromatography, and submitted to biochemical characterization assays. Matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF/MS) analysis revealed that the purified enzyme was a monomer with a molecular mass of 33246.10 Da. The sequence of the 23 N-terminal residues of KERCA showed high homology with those of bacterial keratinases. Optimal activity was achieved at pH 7 and 50 °C. The enzyme was completely inhibited by phenylmethanesulfonyl fluoride (PMSF) and diiodopropyl fluorophosphates (DFP), which suggests that it belongs to the serine keratinase family. KERCA displayed higher levels of hydrolysis and catalytic efficiency than keratinase KERQ7 from Bacillus tequilensis strain Q7. These properties make KERCA a potential promising and eco-friendly alternative to the conventional chemicals used for the dehairing of goat, sheep, and bovine hides in the leather processing industry.0Comments 0Citations
[Show abstract] [Hide abstract] ABSTRACT: Bacillus subtilis HK176 with high fibrinolytic activity was isolated from cheonggukjang, a Korean fermented soyfood. A gene, aprE176, encoding the major fibrinolytic enzyme was cloned from B. subtilis HK176 and overexpressed in E. coli BL21(DE3) using plasmid pET26b(+). The specific activity of purified AprE176 was 216.8 +/- 5.4 plasmin unit/mg protein and the optimum pH and temperature were pH 8.0 and 40 degrees C, respectively. Error-prone PCR was performed for aprE176, and the PCR products were introduced into E. coli BL21(DE3) after ligation with pET26b(+). Mutants showing enhanced fibrinolytic activities were screened first using skim-milk plates and then fibrin plates. Among the mutants, M179 showed the highest activity on a fibrin plate and it had one amino acid substitution (A176T). The specific activity of M179 was 2.2-fold higher than that of the wild-type enzyme, but the catalytic efficiency (k(cat)/K-m) of M179 was not different from the wild-type enzyme owing to reduced substrate affinity. Interestingly, M179 showed increased thermostability. M179 retained 36% of activity after 5 h at 45 degrees C, whereas AprE176 retained only 11%. Molecular modeling analysis suggested that the 176th residue of M179, threonine, was located near the cation-binding site compared with the wild type. This probably caused tight binding of M179 with Ca2+, which increased the thermostability of M179.0Comments 0Citations
- "Some intramolecular interactions among the aromatic 122 th residue, the active site (Asp 32 , His 63 , Ser 220 ), and substrate influenced to increase the catalytic efficiency [23], whereas other substitutions (N217S, A193P, and N160C), which were located far from the active site, did not significantly change the K m or k cat [23]. For a serine alkaline protease from B. pumilus CBS, the catalytic efficiency was increased 42-fold by mutations (L31I/T33S/N99Y) [10]. The substitutions occurred at residues surrounding the catalytic residue, Asp 32 , in serine alkaline proteases. "
0Comments 0Citations
- "However, structural analysis of high-alkaline proteases is still scantly reported. Determination of enzyme 3D structures is also of great importance to get the insights of biophysical and structural forces, which affect the enzyme properties and its function under changing environments [152,153]. "


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A recent Monday at 1:06 p.m.: Zed Shaw flexed his wrists to combat repetitive stress injuries common to computer programmers. Sitting on a ledge at Union Square, he was plugged in to his own world: silver headphones were securely fastened over his ears so that only he could hear his prized sparkle blue guitar. Few paid him any attention, but this silent performance was the highlight of his day. Two years ago, Shaw was writing computer code at a prominent financial institution in New York City, a regular job with a tie and cubicle. The bank failed, and Shaw took a lucrative severance package and headed to San Francisco. He traded the suit and tie for stocking feet and used his knowledge of computer languages to write online how-to books and start his own business. His living room serves as an office where he rattles away on his keyboard answering questions for online students who need help learning code. Shaw loves his new life and freedom to do what he wants when he wants. "The only thing that's missing from my day is people. It's just me. You become a cold weirdo if you don't go around them very much," he said. The solution: going to the square to practice scales and jazz chords. His eyes closed as he relaxed into his practice. Next to him a homeless man slept on a bench. A shirtless guy sunned himself in a chair. A dozen retail workers shared their lunch with a flock of scruffy pigeons. Shaw quietly watched, his headphones shielding him from any interaction. "It's almost like I'm in a fishbowl watching these people go by. I get to meditate and have my solitude but still be around people," he said. "It sort of rehumanizes me."
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