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Native Plants
Q. Who is Mr. Smarty Plants?
A: There are those who suspect Wildflower Center volunteers are the culpable and capable culprits. Yet, others think staff members play some, albeit small, role. You can torture us with your plant questions, but we will never reveal the Green Guru's secret identity.
Did you know you can access the Native Plant Information Network with your web-enabled smartphone?
Ask Mr. Smarty Plants is a free service provided by the staff and volunteers at the Lady Bird Johnson Wildflower Center.
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Saturday - September 20, 2008
From: Austin, TX
Region: Southwest
Topic: Propagation
Title: Propagation by seed of Texas Mountain Laurel
Answered by: Barbara Medford
QUESTION:I just harvested a grocery bag full of Texas Mountain Laurel seed pods. I want to cast them out in a field and wooded area near NW Austin. Is it best to leave the seeds in the pods or remove the pods? Should I put them out now(September) or spring? I do not intend to put in pots, just cast them out on the ground. any recommendations? Thanks, George
Here are the propagation instructions as found on our webpage on Sophora secundiflora (Texas mountain-laurel):
Description: Sow scarified seed after the soil has warmed in spring or fresh seed still swollen in pod in fall. Lightly cover the seed in a pot large enough to allow good root development the first year. A light dusting with a general fungicide is a good precaution to prevent a fungal infection. Mountain laurel seedlings grow slowly the first two years. Cuttings from juvenile trees may root.
Seed Collection: Fruit maturation occurs mid to late summer, but the fruit will remain on the plant through the winter, finally releasing the seed the next summer. Young fruit are large thick, leathery pods that appear brownish gray because of a layer of silky pubescence, which gives the pods a silvery luster. In their second year, the pods weather to become black and thin walled, and soon fall from the plant and deteriorate, eventually releasing the seed. The seed are usually deep red but can be orangish red to almost maroon. They are also very hard. Collect seeds when the pod begins to dry and the seeds turn red
Seed Treatment: Separate seeds from pod and store in bags or containers in a cool dry place. Soaking the hard pods in warm water will soften them and make seed removal easier. Seeds must be filed or mechanically scarified with a knife.
More information on the propagation and care of this plant can be seen on this USDA Forest Service website Sophora secundiflora.
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December 08, 2009 - I am a 5th grade student at Crestwood Elementary School; and one of my classmates came up with an excellent question that I can not answer. Here it is: Why do some plants (like bulbs) resprout in ...
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Bring a buddy.4.53 / 5.00 7,987 Views
Yugi and Kaiba have a duel to the death!4.48 / 5.00 29,839 Views
Vampire Pixel in the Neon City
A sinister deal is underway in the underbelly of Neon City4.36 / 5.00 9,897 Views
This is screwed up and awesome at the same time! I don't know if it was art or an animation of insanity... I think I'll settle on both...
Truely Unique
I love your animation man, my favorite style of flash and art is when it's creepy or morbid, go figure it's in my name. Anyway keep up the the amazing work and amazing animation.
Nice flash
Very good, nicely made flash! The story is great, and the sound fits in it.
whats happened in your childhood to image such those things? :P
Still great works
You have a very strange mind... I love it!!!!
Not my normal kind of video but it worked perfectly for you

subset: commoncrawl


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Home > Terms > English (EN) > cost outlier
cost outlier
A case that is more costly to treat compared with other patients in a particular diagnosis related group. Outliers also refer to any unusual occurrence of cost, cases that skew average costs or unusual procedures.
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East Ascensions stable of running backs could pose problem for Tigers
October 12, 2009 at 9:10 am | Mobile Reader | Print
Tiger running back Alfred Blue rushed for three touchdowns and quarterback Brian Ensminger tossed two more to lead the offensive attack. On defense, lineman Shea Majoria scooped up three fumbles while linebacker Ben Taylor and cornerback Jake Noel both had interceptions.
I thought we played well in the first half, and in the second half we played a lot of backups and they looked good too, Hahnville head coach Lou Valdin said. Vanderbilt Catholic did come out with something new on offense, and managed to pick up some yards on us, but the defense came up with some turnovers.
It was a good game to go into district on because we played better than we did against O.P. Walker the previous week and hopefully we will make more improvement against East Ascension.
East Ascension, the Tigers first district opponent, started out the season with a 35-0 win against Reed, but then dropped their next two contests to Denham Springs and LaGrange. However, Valdin said that East Ascension has been impressive in their last two games, both victories against Ellender and Bonnabel.
They have four really good running backs and they all play, which means they have fresh legs all game, Valdin said. One of them, Avery Williams, runs really hard, and another, Jamarkus Jarvis, is lightning fast and burns people on the outside. He's so fast that he is a lot like Lutcher quarterback Gavin Webster.
On defense, Valdin said that East Ascension has a stout front seven, but if they have a weakness, it would be their secondary.
Turnovers and field position are going to be big, he said. We want to force them to drive the length of the field on us, and to do that, we can't have any turnovers.
Though district play has lost some importance due to the decision to use power rankings to determine playoff teams, Valdin said that it's still a big deal because a district championship is on the line.
We still approach everything the same once we enter district since the power rankings are used now, but a district championship is still on the line, he said. We haven't won one since 2004.
And a win in the first district game is important if that championship is to be achieved.
Half the teams will lose and they still have to play us, Valdin added.
This year, the Tigers will have to win their first district game with the potential distractions of homecoming hanging over their heads.
Hopefully, homecoming will have a positive impact because the players will want to win the game and not get distracted by all the festivities that surround it, Valdin said.
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University study shows UK still prefers face-to-face communication
A study has been conducted by the University of Cambridge that suggests one in three people feel overwhelmed by communications technology.
The study, commissioned by BT to promote the BT Balanced Communications Diet, looked into the impact of texting, email and social networking on the individual and family life; it found that although the majority saw comms technology as having an overall positive effect, for some the wave of innovation in this area seemed to be getting a bit much.
"A survey conducted as part of the study revealed that feeling overwhelmed by communications technology is similar for adults and children, with 38% of 10-18 year olds claiming to feel this way and 25-34 year olds not far behind, with 34% of that age group feeling overwhelmed.
"Furthermore, the survey of 1,269 people and in-depth interviews with families in the UK revealed that those people who have frequently felt overwhelmed are also more likely to feel less satisfied with their life as a whole."
This feeling of being overwhelmed, perhaps, stems not so much from its use - as the majority of people will no doubt manage to find a balance between their real and online worlds - but from the weight of new technology and gadgets being marketed. Often it seems that no sooner have you figured out how to use a swanky new gadget, we're then being lured into buying another, even swankier, one.
However, perhaps the most interesting statistic came through information stating that 64 per cent of children and 65 per cent of adults in the UK still prefer to communicate face-to-face.
Although this could be seen as "dispelling the myth that they [children] only communicate via technology or are losing the desire and ability to participate in in-person interactions", it still hints of the possibility that 35 per cent of the survey preferred some other method of communication other than face-to-face.
However, we still need to be cautious as although it is possible to infer that 35 per cent do not like face-to-face communication, it's important to take into account variables such as inherent shyness, or specific conditions, like aspergers. There's also the fact that there are many alternatives, so it may not mean that 35 per cent do not like face-to-face communication, but that people prefer many different types of communication.
The study remains food for thought, as it's one thing using communication technology because it's convenient and quite another using it because we are uncomfortable with the alternative.
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سکس تهرانی سکس مادر So before he leaves her again she properly wants to say, See you later. سکسی.نغمہ An animatic typically consists of pictures of the storyboard synchronized with the soundtrack. Computers and video cameras in traditional cel animation can also be used as tools without affecting the film directly, assisting the animators in their work and making the whole process faster and easier. سکس تهرانی e. Editing the film at the animatic stage prevents the animation of scenes that would be edited out of the film; as traditional animation is a very expensive and time-consuming process, creating scenes that will eventually be edited out of the completed cartoon is strictly avoided.
Its promotions appear between and near the end of programmes but not in the middle of them, much like the Public Broadcasting Service PBS in the United States and the Australian Broadcasting Corporation ABC in Australia. سکس تهرانی The content of television programs may be factual, as in documentaries, news, and reality television, or fictional as in comedy and drama. سکس تهرانی By contrast, being publicly funded, the BBC in the United Kingdom does not run advertisements, except to advertise its own programmes. سکس ترکی Plot is not still used to develop character and setting, but most of the time, the ultimate goal is not to show scenes of sexuality with few exceptions. سکس مادر This allows the animators and directors to work out any script and timing issues that may exist with the current storyboard.
Adult manga, or hentai manga, is not manga designed for purely pornographic purposes.


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Option Taxation And Margin Requirements - Option Contract Margin Requirements
Investors who purchase option contracts must meet the margin requirements for the position promptly. The investor's margin requirement for the position varies with the type of option position established by the investor. Traditional options have no loan value and investors are required to deposit 100% of the option's premium under Regulation T on T+3 or two business days after settlement. Because option contracts settle the next business day, most brokerage firms require that the investor deposit the required funds by T+1. An investor may purchases a LEAP with an expiration exceeding nine months by depositing 75% of the option's premium in a margin account.
Margin Requirements When Exercising a Call
When an investor exercises a call, the underlying stock transaction will settle regular way on T+3. For settlement and payment dates, the exercise date is considered the trade date. If an investor exercises a call in a cash account, the investor must deposit the entire exercise value of the contract by the payment date. If an investor exercises a call option in a margin account, the required Reg. T deposit must be met by the payment date. An exception to these deposit requirements would be if an investor exercises a call and sells the stock on the same day. This would result in a cashless exercise and would not require additional funds to be deposited.
Margin Requirements When Exercising a Put
When an investor exercises a put, the underlying stock transaction will settle regular way on T+3. If an investor exercises a put in a cash account, the investor must be long the stock in the account or must deposit the stock by settlement date. If an investor is not long the underlying stock and exercises a put option in a margin account, the required Reg. T deposit must be met by the payment date to hold the established short position.
Margin Requirements When Writing a Call
When an investor writes a call, the investor's margin requirement will depend greatly on whether or not the short call is covered. For margin purposes a call is considered covered if the investor is long:
- The underlying stock
- Securities convertible into the underlying stock
- A call option with a lower exercise price and equal or longer expiration
- A warrant with an exercise price lower than the option's exercise price
- An escrow receipt from a bank or trust company showing the stock is on deposit and will be delivered in the case of an assignment.
If the short call is covered by any of the above positions, the option is considered covered and no additional margin deposits will be required. If the investor is long a warrant with an exercise price that exceeds the exercise price of the call, the investor will be required to deposit the difference in the exercise prices in cash to hold the position. If an investor establishes a covered call position with the underlying stock on the same day, the amount of the required deposit is reduced by the option's premium.
An investor establishes the following position in a cash account:
Buy 100 TRY @ 49
Sell 1 TRY Nov 50 call @ 3
The required cash deposit to hold 100 shares of TRY is reduced by the option's premium of $300 from $4,900 to $4,600. Alternatively, if the same position was established in a margin account, the Reg. T required deposit would also be reduced by the option's premium of $300. The Reg. T required deposit to hold the position would be reduced from $2,450 to $2,150. If an investor writes uncovered options, the transaction must be executed in a margin account. The required margin deposit will be subject to the premium received plus a percentage of underlying stock price.
Final thoughts
You have completed the series 4 exam review available on Investopedia. This is only a portion of what you will need to know to for the test. The series 55 can be a challenging exam for many people. This is especially true for people who have not sat on a trading desk. We recommend that you read your textbook two to three times. In addition you should review your notes carefully and take as many series 4 practice questions as you can.
How to Prepare for the Series 4 Exam
For most candidates, the combination of reading the series 4 textbook and taking practice questions proves to be sufficient to successfully complete the exam. It is recommended that the individual spend at least 50 hours preparing for the exam by reading the textbook, underlining key points, and answering as many practice questions as possible. Many series 4 test takers who have challenges can master the concepts with the help of a skilled series 4 tutor
The Securities Institute is a John Wiley & Sons partner company and publishes world class series 4 textbooks and exam prep software. All of us at Investopedia, The Securities Institute & John Wiley & Sons wish you the best of luck on your series 4 exam. Click here For more series 4 exam training materials
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Option MarginThe cash or securities an investor must deposit in his account ...
Call On A CallA type of compound option in which the investor has the right ...
Exercise PriceThe price at which the underlying security can be purchased (call ...
Early ExerciseThe exercise of an option prior to its expiration date. Early ...
Call OverWhen the buyer of a call option exercises the option. In options ...
American OptionAn option that can be exercised anytime during its life. American ...
How do I change my strike price once the trade has been placed already?Learn how the strike prices for call and put options work, and understand how different types of options can be exercised ... Read Answer >>
What are the different types of margin calls?Learn the differences between margin calls and fed margin calls while reviewing the definitions of each and how to satisfy ... Read Answer >>
What are my options when I get a margin call?Understand what a margin call means and the two primary options for meeting a margin call, such as depositing additional ... Read Answer >>
How are call options priced?Learn how aspects of an underlying security such as stock price and potential for fluctuations in that price, affect the ... Read Answer >>
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What does FCOA stand for?
FCOA stands for Fox Cities Officials Association (sports officials)
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- Financial Corporation of America (est. 1989; debt collections; Austin, TX)
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Culture & Religion
April Thu 18, 2013
This past Lent, I decided to do something different. I resolved to read Dante's "Purgatorio." While acquainted with the basics of the Divine Comedy thanks to a college survey course and some "Teaching Company" lectures, it seems I never could really get beyond the bizarrely compelling Inferno. And so, armed with my Cliff's Notes and a freshly loaded Longfellow translation on my iPad, I ventured forth with Virgil to tackle the Seven Story Mountain, catch a glimpse of Beatrice, and maybe even get my own small taste of redemption. The journey indeed proved fruitful. I was most moved at the end. After the arduous ascent and steady removal of the seven "P's" from his forehead, and then passing through the curtain of fire, endured only at Virgil's reminder that Beatrice awaited on the other side, the poet is led into the Earthly Paradise, that blessed land of innocence lost through Adam's sin. Dante sees the beautiful Matilda walking along the banks of a pure stream whose source is no less than the will of God. The stream "opens on two sides:" one, Lethe, takes away "all memory of sin;" the other, Eunoe, restores the memory of "every good deed done." Of the sweetness of these streams, Dante rather understatedly says, "This every other savour doth transcend." Dante must humbly submit to the painful yet just excoriations of the veiled Beatrice. You used to love me, and all the good I signified, says Beatrice... what happened? After I died, you forgot it. You lost your way and chased shadows, betraying your vast potential and your great destiny. A weeping and forlorn Dante can only respond, "The things that present were with their false pleasure turned aside my steps, soon as your countenance concealed itself." Absent Beatrice, he was too weak to resist the siren song of illicit earthly allurements. And so, "pricked with the thorn of penitence," Dante is utterly overpowered by the weight of his misery. He faints; but awakens to find himself up to his neck in the Lethe, Matilda gently ministering to him, tenderly adjuring him to hold onto her as she lowers him into the river, plunges him under, and makes him drink. He rises, his memory purged of all bitter sadness. The unveiled face of Beatrice is at last beheld. Dante gazes at her so fixedly that her maidens must cry, "too intently!" Dante is momentarily blinded by the intensity of the vision of Beatrice. I remember reading in von Balthasar once that Dante was the first Christian writer to theologize eros. I think I at last begin to understand. Dante then sees the mystical procession of the Griffin and the chariot, representing Christ and the Church, and is given an apocalyptic glimpse into the struggles to unfold in salvation history. Finally, he drinks from the Eunoe, has the memory of his good deeds restored, and is ready at last to follow Beatrice into Paradise.
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Home > Press > IAS Forges Nationwide Alliance With Crystal Fusion Technologies, Inc. To Offer Consumers Superior Windshield Protection Technology
IAS adding Crystal Fusion to superior F&I product portfolio in mid December
IAS, a leading provider of F&I aftermarket programs to automobile dealerships, today announced a formal alliance with Crystal Fusion Technologies Inc., a leading provider of windshield protection technologies.
Crystal Fusion is an aftermarket product that helps keep vehicle windshields cleaner and stronger while significantly improving hazards associated with night glare and windshield chips. Crystal Fusion may also increase driver reaction time in inclement weather because of a drastic improvement to overall visibility.
"Crystal Fusion is a perfect addition to the IAS portfolio. It adds substantial value to the vehicle sale while providing the car buyer with a tangible benefit," said Ray Doran, Founder and CEO of Crystal Fusion Technologies Inc. "IAS had all the components in place to expand distribution of Crystal Fusion and we took particular note of their superior reputation for administration and claims."
Under the alliance, IAS has chosen Crystal Fusion exclusively as part of their Windshield Repair or Replacement warranty program offerings. They currently offer a 5 year limited repair or replacement warranty and a general maintenance program. Product availability differs by state.
"Over the course of 2010, we have seen a robust increase in sales for our windshield repair and replacement warranty programs throughout the United States," said Bob Corbin, President and CEO of IAS. "Adding Crystal Fusion to our product offerings made sense for IAS because it's a quality product that will protect our customers as well as have a residual effect on a decrease in claims. Additionally, this opens up a new product for IAS' dynamic reinsurance program."
Crystal Fusion's three step process is simple to install and can take less than ten minutes from start to the delivery of a vehicle. The product is available on both new and used vehicles.
About Innovative Aftermarket Systems
Innovative Aftermarket Systems (IAS) is the leading provider of F&I aftermarket programs to automotive dealerships. IAS offers a variety of products including, GAP, Invisible Phantom Footprints, Theft Avert, Super Polysteel, The Preferred Advantage, R.O.A.D. InTire, among others. Additionally, IAS' flagship software suite, SmartDealerProducts, provides revolutionary F&I software solutions for dealerships and their customers. IAS' training department, SmartDealerUniversity, provides ongoing training and feedback to ensure continued penetration, ethical presentations and increased profits for IAS dealership customers. Headquartered in Austin, Texas, IAS has hundreds of certified agents throughout the United States and provides technology solutions and aftermarket products for many of the top-100 dealer groups. For more information, please visit www.iasdirect.com.
About Crystal Fusion Technologies
Crystal Fusion Technologies, Inc. (CFT) brings advanced nanotechnology to the US Automotive market. The simple 3-step process transforms the surface of windshields so that they easily shed water in the rain, resist chips and cracks, and stay cleaner and clearer longer. CFT and IAS go above and beyond with extended consumer protection, a Maintenance Kit, and bouquet of Performance, Repair and Replacement Warranties to back up the amazing chemical properties of Crystal Fusion Technologies. In addition, the unique Crystal Fusion Maintenance Program builds a bridge between F&I and Service departments while enhancing customer retention.
Headquartered in Long Island, NY, Crystal Fusion's staff includes chemists, glass technicians, and automotive experts, who are committed to making chemically enhanced auto glass available to drivers nationwide, so that safety, durability, clarity and cleanliness can become a mainstay on the American roadway. More at www.cftproducts.com.
For more information, please click here
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A Rose from the Dead (Flower Shop Mystery Series #6)by Kate Collins
View All Available Formats & Editions
In New Chapel, Indiana, flower shop owner Abby Knight worries about her business failing. She needs new customers and knows that flowers and funerals go together like bacon and eggs. Thus she decides to open up a "tasteful" floral booth at the Funeral Director Society's annual convention. On the down side, Abby will deal with some creepy souls who appreciate the DEARLY DEPOTTED as no one else can dig them. For instance, the Colonel who is president of the FDS is the self-anointed Master Mortician who insists he knows everything there is in converting a corpse into a living tribute and is willing to tell anyone who is not afraid to ask him for a consultation. Then there is the environmental ground-hugger Eli "Green Guy" Cotton who believes Kermit is right that grieving relatives should send their loved ones off in green. The lively gala turns morbid when cosmetician to the dead Sybil "the Diva of Death Masks" Blount is killed. Unable to resist, Abby investigates who was drumming up business.
Product Details
- 9781101211267
- Publisher:
- Penguin Publishing Group
- Publication date:
- 12/04/2007
- Series:
- Flower Shop Mystery Series , #6
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- Format:
- NOOK Book
- Pages:
- 304
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- 18 Years
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LEGO Block Party
Ages 4 and up, anyone that can create with the small LEGOS.
Come in on Wednesday afternoons monthly to build with LEGOS.
LEGO creations will be posted on Facebook and Instagram and (if space allows) in the library.
Come to make your own invention or follow our monthly challenge!
Please leave your food/drink and personal LEGOS at home.
Thank you to our generous patrons for the many LEGO donations that helped start this program.
We all have STUFF! Why do we aquire and save more possessions than we need? What happens when our stuff starts to own us? Why can't we just throw things away?
With his upbeat and unique approach, Dave Downs explores the many reasons that our drawers, closets and garages get filled with STUFF. Downs offers helpful hints for managing this difficult problem. Dave uses colorful stories throughout the talk to engage as well as inform the audience. You may recognize a part of yourself in these stories.
Participants will leave knowing that if we are thoughtful about what we acquire, what we keep and what we discard, we will start to reverse the tendency to bury ourselves in "treasures!"
Take Off Pounds Sensibly.
Board Meeting.
General Meeting
Teens in grades 7-12 are eligible to join the East Bridgewater Library TAB. Pick up an application at the Young Adult desk, then join us for our monthly meeting. We'll have food and drinks, meet new people, and help to plan programs for you and your friends at the library!
This grandparent/child gathering each month will include different color experiments, songs, related art projects,
movement with scarves and literature connection stories for you and your 3-5 year old grandchild.
All caregivers are welcome!
Facilitated by Carol Carver, Early Childhood Support Specialist
Self help Inc. CFCE
Artists from Suburbia was founded in 2015 as a new community-based arts venture and proud member of the Eastern Massachusetts Association of Community Theaters. The idea was simple; to take the kind of theatre company
that overruns our major cultural hubs like New York City and Boston, and bring it to the small town of East Bridgewater, Massachusetts. With generous funding from the East Bridgewater Arts Council, we will present three one act plays from local playwrites: The Werewolves, by Alex Moon; The Daffodil Girls by Amanda O'Donnell; and The Moon. And She, by Monica Giordano. We are overwhelmed by the generosity of local cultural councils who continue to fund and support our work, allowing us to reach out to even more communities and offer even more artistic and theatrical opportunities. We will continue to present unique theatrical performances that challenge local artists and audiences to try something new and question what truly defines a theatrical production.
Sign up and check out one of this month's cookbooks at the Main desk.
Pick out a recipe, prepare it, and bring it to the meeting - we'll discuss the books and sample the recipes.
Please let us know which recipe you have selected by June 11th so that we don't have duplicates.
Registration is required.
Fresh Start for Summer!
Come in, TODAY ONLY, with your library card and we will forgive any overdue fines.
Does NOT include lost or damaged items.
Copies of
The Paris Architect
by Charles Belfoure
are available at the main circulation desk of the library.
Join us for an Introduction to Yoga with Kendra Tansey from Maha Yoga Center.
This class is perfect for beginners and will introduce you to the mental and physical benefits of yoga.
Registration is required.
Join Let's Gogh Art for a Creative Canvas Workshop - we'll be working on a Popart Canvas painting!
This workshop is open to kids ages 10-18 and registration is required!
Thanks to the Massachusetts Cultural Council and the East Bridgewater Arts Council for funding this event.
Register Now - Spots are Available!
Plymouth County 4-H Extension is offering an all day babysitting preparedness course.
Must be 11 years and older to attend.
You must pre-register with Plymouth County 4-H.
Copies of
Buddy: How a Rooster Made Me a Family Man
by Brian McGrory
are available at the main circulation desk of the library.
Copies of
Look Again
by Lisa Scottoline
are available at the main circulation desk of the library.
Copies of
All the Light We Cannot See
by Anthony Doerr
are available at the main circulation desk of the library.


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Endocannabinoid receptor CB2 in nonalcoholic fatty liver disease
Version of Record online: 14 DEC 2006
DOI: 10.1111/j.1478-3231.2006.01401.x
Liver International
Volume 27, Issue 2, pages 215–219, March 2007
Additional Information
How to Cite
Mendez-Sanchez, N., Zamora-Valdes, D., Pichardo-Bahena, R., Barredo-Prieto, B., Ponciano-Rodriguez, G., Bermejo-Martínez, L., Chavez-Tapia, N. C., Baptista-González, H. A. and Uribe, M. (2007), Endocannabinoid receptor CB2 in nonalcoholic fatty liver disease. Liver International, 27: 215–219. doi: 10.1111/j.1478-3231.2006.01401.x
Publication History
- Issue online: 14 DEC 2006
- Version of Record online: 14 DEC 2006
- Received 6 August 2006accepted 16 October 2006
- Abstract
- Article
- References
- Cited By
- endocannabinoids;
- liver;
- nonalcoholic fatty liver disease
Background and Aim: Fatty infiltration and fibrosis are major issues in chronic liver disease. Recent reports suggest a role for the endocannabinoid system in these processes.
Aim: To characterize localization and expression of CB2 in normal liver and nonalcoholic fatty liver.
Methods: We studied 64 liver biopsies: eight were considered normal; 56 had a diagnosis of nonalcoholic fatty liver disease (NAFLD); 32 with nonalcoholic steatosis and 24 nonalcoholic steatohepatitis (NASH). CB2 immunolocalization was studied in 38 samples in paraffin blocks using immunohistochemistry, and a computerized semiquantitative analysis was carried out. CB2 mRNA expression was assessed through RT-PCR in 26 frozen liver samples and the ratio CB2/β-actin was used to evaluate differences between groups. Statistical analysis was performed with central tendency measures and the Mann–Whitney U-test. We considered as significant differences those with a P-value <0.05.
Results: Neither parenchymal nor nonparenchymal cells in normal liver tissue react towards anti-CB2 antibodies. All the samples from patients with steatosis and nonalcoholic steatohepatitis showed hepatocellular immunoreactivity. Cholangiocytes were positive only in the NAFLD group. Normal liver tissue showed a normalized CB2/β-actin ratio of 0.001±0.01, steatosis 6.52±17.3 (P=0.05 vs normal) and NASH 6.49±12.2 (P=0.06 vs normal and P=0.6 vs steatosis).
Conclusion: CB2 receptors are expressed by hepatocytes in nonalcoholic fatty liver disease but not in normal liver.


text:
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Welcome to Mobile Add-ons.
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We've updated our Privacy Policy — read about the upcoming changes here. By continuing to use Kickstarter on or after June 9, 2016, you're agreeing to the new policy.
by Justin Reichman
Update #6
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