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Articles tagged einsturzende neubauten
28 Nov 2010 // 10:00 PM
The tale of an uninitiated music reviewer traveling through a world of foreign sonority and coming out potentially scarred for life, and better for it.READ more
19 Dec 2007 // 10:00 PM
The picture we see in 2007 is of a band in complete control of their faculties, to the point where even the seemingly chaotic force of violently exploding sheet metal has been domesticated.READ more
15 Apr 2007 // 11:00 PM
The creation of their music is oftentimes as visually stimulating as it is adventurous, and the well-lit and clear-eyed presentation on this DVD gives the audience a perfect vantage with which to see every percussive stroke of genius.READ more
25 Jul 2005 // 11:00 PM
Of course, there is always something perpetually upsetting about seeing an animated fetus moving through an inky-black universe of German screams and scraping metal.READ more
4 Aug 2004 // 11:00 PM
Einstürzende Neubauten are a force of nature, or, um, a force of industry. The band originated in Berlin as part of Die Geniale Dilletante, an arts collective inspired by the Dadaist arts movement that had reached its boundary-pushing apex in the early years of the second world war.READ more
12 Apr 2004 // 11:00 PM
Perpetuum Mobile follows on the heels of a long tradition of German industrial music from the early days of Kraftwerk all the way to the genre's more recent hard-core incarnation à la Rammstein.READ more
22 Oct 2001 // 4:00 PM
Berlin's Einstürzende Neubauten—that's "Collapsing New Buildings" to the uninitiated—are in many ways an embodiment of a certain stereotype about German art rock.READ more
19 Jun 2000 // 4:00 PM
Many bands attempt to defy or destroy the established musical boundaries. Few succeed, and fewer still do so with the fervor and style of Einstürzende Neubauten.READ more
Kaytranada "Lite Spots" (Singles Going Steady) // Music
Rachael Sage "I Don't Believe It" (video) (premiere) // Music
Macklemore & Ryan Lewis "Dance Off" feat. Idris Elba (Singles Going Steady) // Music
Western Centuries "Hallucinations" (audio) (premiere) // Music
Hitchcock's 'Suspicion', 'I Confess' and 'The Wrong Man' Return in Blu-ray
// Short Ends and Leader
"These three films on DVD from Warner Archives showcase different facets of Alfred Hitchcock's brilliance.READ the article


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Virginia returns to action Sunday against North Carolina.
April 8, 2000
CHARLOTTESVILLE, Va. -- The 60th-ranked University of Virginia women's tennis team fell 5-1 to the 13th-ranked Blue Devils of Duke on Saturday, April 8, at the Sheridan Snyder Tennis Center in Charlottesville. With the loss, the Cavaliers' record falls to 11-8 overall and 1-4 in Atlantic Coast Conference play. Duke improves its record to 15-5 overall and 4-1 in the ACC.
The Cavaliers' lone victory came in singles action. Doubles play began but was suspended due to rain.
The 'Hoos lost five of six singles matches to Duke. In the third singles slot, UVa first-year Henriette Williams (Raleigh, N.C./Broughton) comfortably defeated Hillary Adams 7-5, 6-1. At #1 singles, second-year Amy O'Donnell (Mahwah, N.J./Mahwah) fell 6-3, 6-1 to 21st-ranked Megan Miller. Virginia third-year Christie Schweer (Houston, Texas/Memorial) lost 6-3, 6-1 to 30th-ranked Kathy Sell at #2. Duke's Prim Siripipat defeated third-year Leslie Cook (Boynton Beach, Fla./Lake Worth Christian) 6-4, 6-7 (8), 6-1 at #4. First-year Jennifer Tuchband (Millburn, N.J./Millburn) lost her singles match at #5 in three sets, 6-1, 6-7 (8), 6-1 to Brooke Siebel. Third-year Kelly Weaver (Kingston, N.Y./Kingston) defaulted at #6 singles with a leg injury to Ioana Plesu, who was leading 5-2 in the first set.
Virginia returns to action Sunday, April 9 when it competes against 37th-ranked North Carolina at the Sheridan Snyder Tennis Center. Play is set to begin at 1:00 p.m.
Duke 5, Virginia 1
1. No. 21 Megan Miller (D) def. Amy O'Donnell (UVa), 6-3, 6-1
2. No. 30 Kathy Sell (D) def. Christie Schweer (UVa), 6-2, 6-1
3. Henriette Williams (UVa) def. Hillary Adams (D), 7-5, 6-1
4. Prim Siripipat (D) def. Leslie Cook (UVa), 6-4, 6-7 (8), 6-1
5. Brooke Siebel (D) def. Jennifer Tuchband (UVa), 6-1, 6-7 (8), 6-1
6. Ioana Plesu (D) def. Kelly Weaver (UVa), 5-2 - default
1. Cook and Schweer (UVa) vs. Adams and Miller (D), suspended
2. O'Donnell and Christine Kim (UVa) vs. Sell and Siebel (D), suspended
3. Williams and Tuchband (UVa) vs. Plesu and Siripipat (D), suspended
UVA Athletics Information
In the NewsRecent Headlines News Archives
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Jeff White
Lynch Makes Timely Return to MoundBaseball5/28/16In his first appearance since April 13, freshman Daniel Lynch retired all four batters he faced Friday in Virginia's ACC tournament loss to Wake Forest.Transfers Eager to Help Lift ProgramFootball5/26/16UVA's football team added three transfers this spring: quarterback Kurt Benkert, offensive guard Jared Cohen and defensive end Jack Powers.Cavaliers' Reign Atop NCAA ContinuesMen's Tennis5/25/16With a 4-1 win over Oklahoma on Tuesday night, top-seeded Virginia captured its second straight NCAA men's title and third in four seasons.
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Director of News Content
A 1985 graduate of UVA, White worked at the Richmond Times-Dispatch until July 2009. He was honored six times as the state's Sportswriter of the Year.
More Headlines
Collins Advances To NCAA Singles Semifinals
W. Tennis | 5/28/2016
Virginia senior Danielle Collins advanced to the semifinals of the NCAA Women's Tennis Singles...
Collins Advances To NCAA Singles Quarterfinals
W. Tennis | 5/27/2016
Virginia senior Danielle Collins advanced to the quarterfinals of the NCAA Women's Tennis Singles...
Cavaliers Advance To NCAA Singles/Doubles Sweet 16
W. Tennis | 5/26/2016
Virginia women's tennis players continued to advance at the NCAA Women's Tennis Singles and...
Collins, Elbaba Advance At NCAA Singles Championship
W. Tennis | 5/25/2016
Virginia seniors Danielle Collins and Julia Elbaba both picked up opening-round wins on Wednesday...
Virginia Begins NCAA Tournament Individual Play Wednesday
W. Tennis | 5/24/2016
Members of the Virginia women's tennis team begin play in the NCAA Singles and Doubles...
Collins, Elbaba Named National ITA Award Winners
W. Tennis | 5/24/2016
Virginia women's tennis seniors Danielle Collins and Julia Elbaba were named national ITA Award...
No. 14 Virginia Falls To No. 6 Vanderbilt In NCAA Quarterfinals
W. Tennis | 05/21/2016
No. 14 seed Virginia's season ended Saturday with the program's second-ever appearance in the...
No. 14 Virginia Advances With 4-2 Win Over No. 3 North Carolina
W. Tennis | 05/19/2016
The No. 14 seeded Virginia women's tennis team defeated third-seeded North Carolina 4-2 to...
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Photo posted on: 30th May, 2016
Happy Memorial Day Today we remember those who have sacrificed their lives for our great country. We hope you have a great Memorial Day!
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10 Tips to Help the Dishwasher Run Better
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Learning to Avoid Common Diet Traps
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Flappy Goku
- The flappy battle rages on. This time you have to help the hero of Dragon Ball, Son-Goku, to run and jump through a parkour of obstructive bricks walls in order to reach the maximum distance. Avoid to fail or FLAPPY GOKU has to restart with a bad headache.
- Game Rating:
- Category: Action
- Launched: 2014-03-13
- Played: 1,015 views
- Tags: 1 player, Action, dragon ball, funny, hardcore, hero, skill
How to Play Flappy Goku
- Mouse = Flap / Jump
Flappy Goku Review
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The first two authors contributed equally to this work
Immunity to infection
Expression and function of NKG2D in CD4+ T cells specific for human cytomegalovirus
Version of Record online: 16 NOV 2006
DOI: 10.1002/eji.200636682
Copyright © 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
European Journal of Immunology
Volume 36, Issue 12, pages 3198–3206, December 2006
Additional Information
How to Cite
Sáez-Borderías, A., Gumá, M., Angulo, A., Bellosillo, B., Pende, D. and López-Botet, M. (2006), Expression and function of NKG2D in CD4+ T cells specific for human cytomegalovirus. Eur. J. Immunol., 36: 3198–3206. doi: 10.1002/eji.200636682
Publication History
- Issue online: 27 NOV 2006
- Version of Record online: 16 NOV 2006
- Manuscript Accepted: 20 OCT 2006
- Manuscript Revised: 9 OCT 2006
- Manuscript Received: 5 SEP 2006
Funded by
- Ministerio de Educación y Ciencia. Grant Number: SAF2004–07632
- DURSI (Generalitat de Catalunya)
- Abstract
- Article
- References
- Cited By
- Cytomegalovirus;
- CD85j;
- T cell
- Top of page
- Abstract
- Introduction
- Results and Discussion
- Concluding remarks
- Materials and methods
- Acknowledgements
The human NKG2D killer lectin-like receptor (KLR) is coupled by the DAP10 adapter to phosphoinositide 3-kinase (PI3 K) and specifically interacts with different stress-inducible molecules (i.e. MICA, MICB, ULBP) displayed by some tumour and virus-infected cells. This KLR is commonly expressed by human NK cells as well as TCRγδ+ and TCRαβ+CD8+ T lymphocytes, but it has been also detected in CD4+ T cells from rheumatoid arthritis and cancer patients. In the present study, we analysed NKG2D expression in human cytomegalovirus (HCMV)-specific CD4+ T lymphocytes. In vitro stimulation of peripheral blood mononuclear cells (PBMC) from healthy seropositive individuals with HCMV promoted variable expansion of CD4+NKG2D+ T lymphocytes that coexpressed perforin. NKG2D was detected in CD28– and CD28dull subsets and was not systematically associated with the expression of other NK cell receptors (i.e. KIR, CD94/NKG2 and ILT2). Engagement of NKG2D with specific mAb synergized with TCR-dependent activation of CD4+ T cells, triggering proliferation and cytokine production (i.e. IFN-γ and TNF-α). Altogether, the data support the notion that NKG2D functions as a prototypic costimulatory receptor in a subset of HCMV-specific CD4+ T lymphocytes and thus may have a role in the response against infected HLA class II+ cells displaying NKG2D ligands.
- aNKR:
activating NKR
human cytomegalovirus
killer immunoglobulin-like receptor
killer lectin-like receptor
natural killer receptor
rheumatoid arthritis
UL16-binding proteins
- Top of page
- Abstract
- Introduction
- Results and Discussion
- Concluding remarks
- Materials and methods
- Acknowledgements
Some T lymphocyte subsets share with NK cells the expression of inhibitory and activating receptors specific for HLA class I molecules, such as killer immunoglobulin-like receptors (KIR), CD94/NKG2A, CD94/NKG2C and ILT2 (LIR1, CD85j) 1–4. NK cell receptors (NKR) have been detected in TCRγδ+ cells as well as TCRαβ+ CD8+ and CD4+ lymphocytes with an effector-memory phenotype 5–8, including virus-specific cytotoxic T lymphocytes (CTL) 9, 10. The molecular basis regulating NKR expression along T cell differentiation is not completely understood, but there is evidence supporting the involvement of cytokines and TCR-dependent signals, and it has been hypothesized that NKR expression results from chronic antigenic stimulation 6, 11.
Inhibitory NKR (iNKR) are believed to prevent T cell-mediated autoreactivity and counterbalance the action of activating NKR (aNKR) 6, 12. Most aNKR (i.e KIR and CD94/NKG2C) are coupled by DAP12 to protein tyrosine kinase (PTK) activation pathways 4 and may trigger or costimulate T cell proliferation and effector functions 13–15. By contrast, the human NKG2D killer lectin-like receptor (KLR) is linked by DAP10 to a phosphoinositide 3-kinase (PI3 K) activation pathway 16, 17; NKG2D functions as a costimulatory molecule in T cells 18, 19 but may activate NK cells and IL-15-stimulated intraepithelial T lymphocytes in a TCR-independent manner 20, 21.
Human NKG2D interacts with several stress-inducible ligands including MICA, MICB and a family of proteins termed "UL16-binding proteins" (ULBP) that are expressed by some normal tissues, tumour cells and virus-infected cells 16, 19, 22–25. The KLR activates NK cells and costimulates the response of CD8+ CTL against human cytomegalovirus (HCMV)-infected targets 18, 26; the identification of immune evasion mechanisms that interfere with surface expression of NKG2D ligands underline its importance in the antiviral response. The UL16 glycoprotein inhibits surface expression of MICB, ULBP1, ULBP2 22, 27–29 and also interacts with RAET1G 24. The gpUL142 HCMV molecule has been recently reported to down-regulate MICA 30.
Human NKG2D was originally identified on NK cells, TCRγδ+ cells and TCRαβ+CD8+ T lymphocytes, but CD4+NKG2D+ T cells have been described in rheumatoid arthritis (RA) and some cancer patients 31, 32. Goronzy and Weyand 33 hypothesized that CD4+ T lymphocytes expressing NKG2D and aNKR represent senescent effector-memory T cells that may contribute to the pathogenesis of RA and other chronic inflammatory disorders 15. NKG2D might exacerbate RA progression by reacting with its ligands abnormally expressed by the inflamed synovium 31, 33. On the other hand, stimulation by soluble MIC molecules (sMIC) has been proposed to account for the increased frequencies of CD4+NKG2D+ T cells producing Fas ligand in patients bearing MIC+ tumours 32. Recently, CD4+NKG2D+ T cells have been identified in patients with human T cell lymphotropic virus type I (HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) 34.
In the present study, we provide the first evidence supporting the notion that a subset of HCMV-specific CD4+ T cells displays NKG2D. Remarkably, expression of the KLR was not systematically associated with expression of other NKR (i.e. ILT2, KIR and CD94/NKG2). Engagement of NKG2D costimulated TCR-dependent proliferation and cytokine production, indicating that the KLR may contribute to the response of a subset of HCMV-specific CD4+ T lymphocytes against infected HLA class II+ cells bearing NKG2D ligands.
Results and Discussion
- Top of page
- Abstract
- Introduction
- Results and Discussion
- Concluding remarks
- Materials and methods
- Acknowledgements
Expression of NKG2D on HCMV-stimulated CD4+ T cells
Human NKG2D is commonly displayed by NK cells as well as TCRγδ+ and TCRαβ+CD8+ T lymphocytes, but it has also been detected in TCRαβ+CD4+ cells from RA and cancer patients 31, 32. In the present study, we analysed the expression of NKG2D in HCMV-specific CD4+ cells. In agreement with previous reports 35, T cell proliferation was detected when peripheral blood mononuclear cells (PBMC) from healthy HCMV-seropositive (HCMV+) blood donors (n=9) were cultured in the presence of the virus. Cells recovered from HCMV-stimulated samples were predominantly CD4+ T lymphocytes (84±7%), which displayed an oligoclonal pattern of TCR rearrangement (data not shown), consistent with the expansion of T cells specific for viral antigens 35; no response was observed in HCMV-seronegative (HCMV–) individuals (n=3) (data not shown).
As compared to fresh PBMC and to control samples cultured in the absence of the virus, substantial numbers of HCMV-stimulated CD4+ lymphocytes displayed NKG2D (Fig. 1); the proportion of CD4+ T lymphocytes expressing NKG2D on day 10 of stimulation varied widely in different HCMV+ donors (n=9; mean ± SD, 21±15%; range, 1–75%) and were rare (⩽4%) in the absence of HCMV stimulation. The expansion of NKG2D+ cells was comparable upon incubation with different HCMV strains (i.e. Towne and AD169) and was undetectable in samples from HCMV-seronegative donors (not shown). Similar results were obtained using purified or UV-inactivated virus preparations (not shown).
Distribution of ILT2, KIR and CD94/NKG2 NKR on HCMV-stimulated CD4+ T cells
Flow cytometry analysis carried out in parallel with a panel of mAb specific for different NKR indicated that ILT2+ and KIR+ cells were also increased among HCMV-stimulated CD4+ T cells (Fig. 1); by contrast, few CD4+CD94/NKG2+ cells were detectable (Fig. 1 and data not shown). Remarkably, the expression of NKG2D, ILT2 and KIR did not systematically coincide. In fact, when expression of NKG2D and the ILT2 inhibitory receptor were compared on HCMV-stimulated CD4+ cells, distinct distribution patterns were observed. In most cases (n=6), CD4+ILT2+ and CD4+NKG2D+ cells were detected (Fig. 2A, donor #2), and three-colour flow cytometry analysis revealed the existence of a subset coexpressing both molecules (Fig. 3A). However, in some individuals only CD4+ cells selectively bearing either NKG2D (n=1) or ILT2 (n=2) were expanded (Fig. 2A, donors #3 and #4). When samples from several representative donors (n=5) were reanalysed 4–10 months later, the distribution patterns observed in the first study were reproduced in every case, regardless of the variability in the proportions of recovered NKG2D+ or ILT2+ cells; the phenotypes of HCMV-stimulated CD4+ cells from two individuals (#2 and #3) studied at different time points are shown for comparison (Fig. 2A, B).
The complex diversity of KIR haplotypes and, particularly, the inability of the available mAb to discriminate homologous activating and inhibitory KIR 1 did not allow precise analysis of the relationship between individual members of this receptor family and NKG2D. Yet, two-colour analysis of CD4+ cells using a mixture of different anti-KIR mAb as described 36 also ruled out coordinated expression of NKG2D with these receptors (Fig. 2B).
The dissociated distribution of NKG2D, KIR and ILT2 in HCMV-specific CD4+ cells indicates that their expression is differentially regulated. NKG2D was reported to be induced under the influence of cytokines (i.e. IL-15) and TCR-dependent stimulation 31, 37; furthermore, it has been proposed that NKG2D engagement by soluble MIC molecules promotes the expansion of CD4+NKG2D+ cells in cancer patients bearing MIC+ tumours 32. KIR and ILT2 were shown to be displayed by effector-memory T cells 6, 8, yet the mechanisms regulating their expression remain poorly defined. CD8+ T cells bearing inhibitory NKR have been reported to be increased in HIV-infected patients 38. ILT2+ T cells have also been observed to be augmented during HCMV infection in transplant patients 39 as well as in HCMV+ blood donors 36. ILT2 was detected in HCMV-specific CD8+ T cells 9, 10 and in CD4+ T lymphocytes responding to Mycobacterium tuberculosis antigens 40. Our results provide a first indication that ILT2 may also be expressed by HCMV-specific CD4+ T cells. ILT2 interacts with different HLA class I molecules and with the UL18 HCMV glycoprotein 41, 42. The regulatory function of ILT2 in the response of CD4+ T cells against HCMV-infected cells deserves further attention.
CD4+NKG2D+ T cells are CD28– or CD28dim cytotoxic T lymphocytes
Additional phenotypic studies using three-colour flow cytometry analysis revealed that CD4+NKG2D+ lymphocytes express perforin (Fig. 3B) and granzyme B (data not shown), thus corresponding to a described subset of HCMV-specific CD4+ CTL 43; it is noteworthy that both perforin and granzyme B were also detected in CD4+NKG2D– cells (Fig. 3B; data not shown). Considering the costimulatory function of NKG2D in CD8+ cells, we compared its distribution to that of CD28. Both molecules appeared dissociated in HCMV-stimulated CD4+ lymphocytes (Fig. 3C), but a NKG2D+CD28dull subset was detectable in some samples (Fig. 3C). These observations suggest that HCMV-specific CD4+ T cells, previously shown to display an effector-memory phenotype 44, 45, may switch the use of costimulatory receptors, becoming CD28– NKG2D+.
NKG2D functions as a costimulatory receptor in a subset of HCMV-specific CD4+ T cells
To verify whether NKG2D+ cells were specific for HCMV antigens, CD4+NKG2D+ and CD4+NKG2D– subsets were sorted and restimulated with the virus in the presence of irradiated (40 Gy) autologous PBMC. A specific proliferative response and IFN-γ production were detected in both populations (Fig. 4), thus indicating that CD4+NKG2D+ cells represent only a fraction of HCMV-specific T lymphocytes, as predicted by the phenotypic analyses described above. Attempts to grow CD4+NKG2D+ T cell clones were unsuccessful, suggesting that their proliferative capacity is limited, in line with previous studies showing that HCMV-specific T cells undergo replicative exhaustion 46.
To assess the function of NKG2D, HCMV-stimulated CD4+ T cells were incubated with suboptimal concentrations of anti-CD3 mAb in the presence or absence of anti-NKG2D mAb. Under these conditions, engagement of the KLR synergized with TCR-dependent signals, efficiently triggering proliferation and cytokine production (Fig. 5). These results support the notion that NKG2D functions as a prototypic costimulatory receptor in HCMV-specific CD4+ cells. In line with this, the KLR has recently been shown to be coupled to the DAP10 adapter in CD4+NKG2D+ cells derived from cancer patients 32.
The design of an in vitro experimental system suitable to directly study the costimulatory role of NKG2D and other NKR (i.e. ILT2) in the response of CD4+ T cells to HCMV-infected cells is warranted, though some technical limitations must be overcome. It is noteworthy that fibroblasts, which are commonly used to analyse the response to HCMV, do not express HLA class II molecules, and other susceptible cell types (i.e. endothelial and hemopoietic cells) are far less permissive to in vitro infection 47.
Collectively, our data suggest that CD4+NKG2D+ cells expanding in HCMV-stimulated cultures correspond to virus-specific memory T cells that have acquired NKG2D while losing CD28. By switching the use of costimulatory molecules, CD4+ cells primed by professional antigen-presenting cells (APC) might efficiently respond to other HLA class II+ virus-infected cell types. Though most studies have focused on the response of CD8+ CTL to HCMV antigens 48, CD4+ T cells specific for epitopes of viral proteins such as pp65 and IE1 have been identified 49–51. Recently, the gB HCMV glycoprotein was shown to be processed via the endosomal pathway by non-professional APC, being efficiently presented by HLA class II molecules to CD4+ T cells 52. HLA class II expression in different HCMV-infected cell types may be either constitutive (i.e. macrophages) or inducible by proinflammatory cytokines, and it is impaired by some virus molecules 53. On the other hand, NKG2D ligands are displayed in HCMV-infected cells, and immune evasion mechanisms that interfere with their expression indirectly reflect the importance of the KLR in the anti-viral response 18, 22, 27–29.
CD4+NKG2D+ T cells were originally described in RA patients 31. Goronzy and Weyand hypothesized that immunosenescence contributes to the development of RA and proposed that expression of NKG2D and aNKR (i.e. KIR2DS2) may lower the activation threshold of senescent CD4+CD28– T lymphocytes, favouring their participation in the pathogenesis of the disease. NKG2D might exacerbate RA upon interaction with its ligands expressed by the inflamed synovium 31, 33. A role for NKR+ T cells in the development of other chronic inflammatory disorders such as atherosclerosis has also been suggested 15. It is noteworthy that HCMV infection is considered to be a major contributor to the immunosenescence process 54. According to our observations, the possibility that the increased numbers of CD4+NKG2D+ T cells found in RA and cancer patients 32 may represent HCMV-specific T cells should be envisaged. Frequent subclinical reactivation of the virus, favoured by the immune dysfunction and/or immunosuppressive therapy in these patients, might account for the increased proportions of CD4+NKG2D+ T cells. On the other hand, the possibility that this T lymphocyte subset may also expand in response to different antigens, including other microbial pathogens, is not ruled out. Regardless of their primary antigenic specificity, NKG2D would enhance the response of potentially autoreactive CD4+ T cells against non-infected tissues, where expression of its ligands may be either constitutive or inducible by a variety of stimuli 26, 55, 56.
NKG2D has been detected in murine NK cells, CD8+ T lymphocytes and macrophages, and it has been shown to participate in the immune response against murine cytomegalovirus (MCMV) 19, 57 as well as in the pathogenesis of experimental autoimmune type I diabetes 58. The expression of NKG2D in murine effector-memory CD4+ CTL should be carefully reassessed.
Concluding remarks
- Top of page
- Abstract
- Introduction
- Results and Discussion
- Concluding remarks
- Materials and methods
- Acknowledgements
Our results provide first evidence supporting the notion that human NKG2D functions as a costimulatory molecule in a subset of HCMV-specific CD4+ T lymphocytes and thus may contribute to their response against HLA class II+ virus-infected cell types displaying NKG2D ligands. The possibility that the increased numbers of CD4+NKG2D+ T cells found under some pathological conditions may be primarily specific for HCMV or other microbial pathogens should be envisaged. Further studies are required to explore the putative role of CD4+NKG2D+ cells in the pathogenesis of chronic inflammatory disorders. In this regard, the dissociated expression of NKG2D and inhibitory NKR (i.e. ILT2, KIR) in CD4+ T lymphocytes deserves special attention, as it might increase the risk of autoimmune reactions.
Materials and methods
- Top of page
- Abstract
- Introduction
- Results and Discussion
- Concluding remarks
- Materials and methods
- Acknowledgements
Heparinized blood samples were obtained from healthy adult individuals. Written informed consent was obtained from every donor, and the study protocol was approved by the Ethics Committee (CEIC-Institut Municipal d'Assistencia Sanitaria). As described 36, standard clinical diagnostic tests were used to analyse serum samples from blood donors for circulating IgG antibodies against CMV (Abbot Laboratories, Abbot Park, IL); nine HCMV-seropositive (HCMV+) and three seronegative (HCMV–) donors were studied.
HCMV preparations
As described 59, AD169 and Towne strains of HCMV were propagated in HFF or MRC-5 fibroblast cell lines following standard procedures. Viral titres were determined by standard plaque assays on MRC-5 cells. The AD169 strain of virus was inactivated under UV light as described 59. The purified AD169 strain of HCMV was obtained from ABI Advanced Biotechnologies Inc. (Columbia, MD).
Lymphocyte cultures
Culture medium was RPMI 1640 with Glutamax-I and 25 mM Hepes (Gibco, UK), supplemented with 10% v/v heat-inactivated fetal calf serum (FCS), penicillin (100 U/mL) and streptomycin (10 μg/mL), referred to as complete medium. PBMC obtained from heparinized blood by Ficoll-Hypaque gradient centrifugation (Lymphoprep, Axis-Shield PoC AS, Oslo, Norway) were incubated in 24-well plates (2 × 106 cells/well) in complete medium supplemented with 10 U/mL human recombinant interleukin-2 (hrIL-2; Proleukin, Chiron, Emeryville, CA) either alone or in the presence of cell-free HCMV (2 × 105 PFU/well). Cell cultures were maintained at 37°C in a 5% CO2 humid atmosphere for 10–12 days; every 3 days 50% of the supernatant was replaced with fresh medium supplemented with IL-2; when high cell density was attained, cell cultures were split.
HP-3E4 anti-KIR2DL1/S1/S3, HP-3B1 anti-CD94, HP-F1 anti-CD85j mAb and BAT221 anti-NKG2D were generated in our laboratories 23, 36. 5.133 anti-KIR3DL1/L2 and KIR2DS4 was provided by Dr. Marco Colonna. Z199 anti-NKG2A and ON72 anti-NKG2D mAb were provided by Dr. Alessandro Moretta (University of Genova, Italy). Dx9 anti-KIR3DL1 mAb was provided by Dr. Lewis Lanier (UCSF, San Francisco, CA). CH-L anti-KIR2DL2/S2/L3 was provided by Dr. Silvano Ferrini (University of Genova, Italy). 3A1 anti-CD7 has been described 60, and anti-NKG2C (MAB1381) was from R&D Systems (Minneapolis, MN). Indirect immunofluorescence analysis was carried out with PE- or FITC-tagged F(ab')2 rabbit anti-mouse Ig antibodies (Dakopatts, Glostrup, Denmark) or allophycocyanin (APC)-labelled goat anti-mouse Ig (BD Biosciences Pharmingen). The following fluorochrome-tagged mAb were used for multicolour staining: CD4-PE, CD4-FITC, CD28-PE, anti-human Perforin-FITC and FITC-conjugated mouse anti-human Granzyme B (BD Biosciences Pharmingen). HP-F1 was labelled with biotin using EZ-Link Sulfo-NHS-Biotin (Pierce) according to the manufacturer's instructions and was used in combination with streptavidin-FITC (BD Biosciences Pharmingen).
Immunofluorescence and flow cytometry analysis
Multicolour immunofluorescence and flow cytometry analysis was performed as described 36. Briefly, cells were pretreated with saturating concentrations of human aggregated Ig to block FcR and were then incubated with the individual unlabeled mAb. After washing, samples were labelled either with FITC-tagged F(ab')2 rabbit anti-mouse Ig antibody (Dako) or allophycocyanin (APC)-labelled goat anti-mouse (BD Biosciences Pharmingen). In some experiments, cells were incubated with biotin-labelled HP-F1 followed by streptavidin-FITC (BD Biosciences Pharmingen). Subsequently, samples were washed and incubated with PE- or FITC-conjugated antibodies (BD Biosciences Pharmingen). Flow cytometry analysis was carried out as described (FACScan, Becton Dickinson, Mountain View, CA).
For multicolour intracellular staining, the BD Cytofix/Cytoperm Kit was used (BD Biosciences Pharmingen). Briefly, cells were stained with ON72 anti-NKG2D and allophycocyanin (APC)-labelled goat anti-mouse Ig, followed by CD4-PE. Samples were fixed, permeabilised following the manufacturer's instructions and stained with anti-human Perforin-FITC or FITC-conjugated anti-human Granzyme B mAb (BD Biosciences Pharmingen). Cells stained with CD4-PE alone or in combination with BAT221 anti-NKG2D and FITC-tagged F(ab')2 rabbit anti-mouse Ig, as described above, were sorted under sterile conditions (FACSVantage, Becton Dickinson, Mountain View, CA) and used in functional assays.
Cytokine production and cell proliferation assays
NKG2D+CD4+ and NKG2D–CD4+ HCMV-stimulated cells were sorted (FACSVantage, BD), resuspended in complete medium and cultured in 96-well plates (105 cells/well) with irradiated (40 Gy) autologous PBMC (2 × 105 cells/well), either alone or in the presence of HCMV (104 PFU/well). In other experiments, CD4+ cells were sorted and stimulated with mAb preadsorbed to culture plates as described 13. Briefly, 96-well plates were coated with sheep anti-mouse Ig (10 μg/mL) overnight at 4°C, washed with PBS and incubated with specific antibodies for 3 h at room temperature. The following mAb were used: 3A1 anti-CD7 (10 μg/mL), SpvT3b anti-CD3 (2 ng/mL) and ON72 anti-NKG2D (50 μL hybridoma supernatant). After washing plates, CD4+ sorted cells (105/well) were added in complete medium; each experimental condition was set up in triplicate. In every case, supernatants were harvested after 48 h, and production of IFN-γ and TNF-α was analysed by ELISA (Human IFNγ Module Set and Human TNFα Module Set; Bender MedSystems, San Bruno CA). As described 13, 3[H]-Thymidine (3HTdR) was added (1 μCi/well) at 48 h, and cultures were further incubated for 18 h at 37°C; cells were harvested, and 3HTdR incorporation was measured in a β-counter (LKB Wallac, Turku, Finland).
- Top of page
- Abstract
- Introduction
- Results and Discussion
- Concluding remarks
- Materials and methods
- Acknowledgements
This work was supported by grants from the Ministerio de Educación y Ciencia (SAF2004–07632 and SAF2005–05633). A. S-B- is supported by a fellowship from DURSI (Generalitat de Catalunya). We are grateful to Dr. Oscar Fornas for advice in flow cytometry analysis as well as to Gemma Heredia and Carmen Vela for technical support.
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text:
Written on: 16/01/2014
As researching a reasonable priced deals for holidays, someone suggested malta and this company for there deals. We booked Bella Vista in Qwawra-turnout to be good, more like 3 star,but malta is no england. Few bars,restaurants around, we hired a car for a week as wanted to see the island and go to other bars and restaurants bit more far away.great week,nice weather,hotel also nice. Oh and the company was good too,we had no problems with there service.We will book soon again .
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text:
Asking Alexandria Cover NIN
Why do they hate everyone who isn't deaf
"Asking Alexandria"
I died a little inside.
Oh good, it's basically just the original song with added breakdowns and girls. This band in a nutshell really.
From what I've heard they're music is appalling. I just listened to a song of theirs on YouTube and well... The crunchy metalcore breakdowns + the faulty electronic music = a total case of explosive diarrhea.
Possibly the worst piece of "music" I've ever heard. And I've heard som incredibly bad stuff.
Digging: First Fragment - Dasein
And this is part of a new Punk Goes album? Well doesn't this just keep getting better and better
Musically-retarded bastards. When will they get their shit straight? lol
oh god, it's here
Digging: Darkend - The Canticle of Shadows
it's basically just the original song with added breakdowns and girls [2]
what the fuck is this shit
unaMUSEd=good name. GOOD FUCKING NAME.
is there a problem with my name.
No? I love it. Like literally made me chuckle and smile.
better than the original wow theyre awesome
Oh... sarcasm is so common on this site especially when caps are involved so I wasn't sure.
Yeah, I understand. Sput's users are overly sarcastic sometimes.
Jesus Christ
Asking Alexandria to fucking stop
Digging: Melvins - Bullhead
Other than this song being atrocious, Asking Alexandria accomplished something. They didn't have a breakdown in the beginning of the song, and finally had one at 2 minutes. That's a musical achievement for these guys.
the most offensive news article ive ever seen
Digging: Vektor - Terminal Redux
did trent reznor have to approve this???
They didn't do anything interesting with this cover, which defeats the point of doing one in the first place. I mean, outside of partaking in an obvious cash grab.
"They didn't do anything interesting with this cover, which defeats the
point of doing one in the first place. I mean, outside of partaking in an
obvious cash grab."
Which is kind of the point of the band entirely, at this point.
Why would anyone allow this? Way to go asking alexandria, you dumbfucks.
Sounds like some talentless x factor audition. They hate everyone with ears.
The thing that kills this for me are the synths. They sound so incredibly cheap, especially compared to the original.
the funny thing is that it's actually not as terrible as i thought it would be
I think Trent approved this as well lol
maybe trent just needed a good laugh
Ok, now that's definitely crossing the line.
Somebody needs to put an end to this.
This song was like putting down the family dog.
Digging: The Fall of Troy - OK
Started off alright-ish, in my view it was just an unoriginal and crappy copy of it which stayed pretty true to the original. And then in came the beatdowns. Downhill from there.
even the original of that song suck and this is pretty much horrible
danny's screams are now as bad on record as they are live. They sound so hollow. At least his lower register sounds good, cause his higher register is pretty much fucked now (raspy in a bad way).
Solid band
I don't want to know how this goes
The cover was actually okay until the fucking chugs and squeals were brought in.
Digging: Tiny Moving Parts - Celebrate
It's unreal that this is six minutes long
terribly terrible
Jesus shit that guitar tone is horrendous and the fucking "1 0" chugs.
the actual song is six minutes long though
brb puking
I don't want to know how they recreated the last three minutes
Oh God, I bet some girls out there think they wrote the song too.
scene girls probably don't even know who trent is
Digging: Chance the Rapper - Coloring Book
yeaaaaaaaah no. sounds really similar to the original
The added lyrics made me want to die.
Oh look they take the "fuck you like an animal" part and use literal animal imagery... so fucking clever.
Sorry Snide, can't agree with you on this one... the whole point of Nine Inch Nails is their dark understated tone. This
started off promising but the breakdown just ruined it for me.
Hahahaha. Hahah. Asking Alexandria. Just. Fuck em.
This is the worst kind of cover too. It wants to sound exactly like the original. This is just piss. It's not even good enough to be shit.
Oh god, then comes the added lyrics and "breakdown". This is hilarious.
lol those lyrics at the end are in the real song
They ruined this song for me.
Digging: Saosin - Along the Shadow
Exactly as expected
"lol those lyrics at the end are in the real song"
Goddammit you're right... well I feel stupid now. But my main point still stands... this tries too hard to be just like the original and yet at the same time manages to miss the tone of the original completely. I honestly can't believe Trent approved this.
snide loves bvb and aa
scene girls probably don't even know who trent is [2]
It started OK but then I guess you could say it went into a Downward Spiral.
Snide why's your taste so "teenage scene girl".
Oh, I'd forgotten what he spoke towards the end. Well, it still stands, the delivery made me want to die.
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LDS Church buys farmland, Haun's Mill, Far West, Kirtland property from Community of Christ
Kenny Mays
SALT LAKE CITY — The Church of Jesus Christ of Latter-day Saints confirmed Saturday that it purchased 6,000 acres of Missouri farmland and three historical sites from the Community of Christ — the group formerly known as the Reorganized Church of Jesus Christ of Latter-day Saints.
"The Church recently acquired operating farmland and several other non-farmland properties located in Missouri and Ohio from the Community of Christ," LDS Church spokesperson Scott Trotter said. "Non-farm sites include the Haun's Mill and the Far West Burying Ground in Missouri as well as the Joseph Smith Sr. home in Kirtland, Ohio."
Trotter specified that no plans exist to develop the properties; farm operations will continue and historic sites will be maintained.
Situated in Caldwell County, Mo., Haun's Mill is remembered in LDS history as the place where at least 18 Mormons were massacred on Oct. 30, 1838, when Caldwell County sheriff William Jennings led an armed militia of more than 200 men into the small settlement and had them open fire without warning.
Also located in Caldwell County, Far West is the settlement Mormons founded in 1836 that served as the church's headquarters during most of 1838. It's estimated that more than 200 early Latter-day Saints are buried at the Far West Burial Grounds, including original apostle David W. Patten.
Joseph Smith Sr. resided in Kirtland from 1831-38, and was living there at the time he was called in 1833 to be the church's first Presiding Patriarch.
When contacted by KSL-TV regarding the sale, the Community of Christ issued a statement that included, "Ongoing preservation is Community of Christ's paramount concern for Haun's Mill, the Far West Burying Ground and the Smith Sr. home in particular."
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I'm heading to Columbus, Ohio, for the official opening of Arrow's newly automated facility for processing electronics at end of life. I'm hoping to turn this into an opportunity to meet sustainability leaders in the greater Columbus area. Here in Boston, I co-organize the Boston Area Sustainability Group, knowing that continuing to connect with other…Continue Reading →
Arrow Value Recovery Blog
Centennial, Colo. – May 18, 2016 – Arrow Electronics, Inc. (NYSE: ARW), a global leader in IT asset disposition, today announced it upgraded its Columbus, Ohio-area value recovery facility, adding state-of-the-art automated equipment and a new layout to further increase processing efficiency and chain-of-custody security. As a result of the upgrades, the facility's IT asset processing…Continue Reading →
"And there are those who do not yet know that they are good." This quote is from Mandar Apte, one of the panelists on my "Building Substantive and Collaborative Networks" panel at Sustainatopia in San Francisco earlier this month. In his years at Shell Oil at the intersection of Sustainability and Innovation, Mandar learned that…Continue Reading →
I fell in love with the CleanMed conference when I first attended in 2010. CleanMed is a content-rich gathering, chock full of heart. It was started by two determined nonprofits, Healthcare without Harm and Practice Greenhealth, in 2001. For my money, CleanMed is positively the most inspiring of conferences. As the premier national conference for…Continue Reading →
Article featured in JustMeans: The sustainability expert for Arrow Electronics' value recovery business, Carol Baroudi, was a featured speaker earlier this week at Sustainatopia, a multi-day event focused on social, financial and environmental sustainability and impact. Baroudi moderated a panel on "Building Substantial and Collaborative Networks" where participants discussed the need to grow one's professional…Continue Reading →
Data sanitization is key to recovering value in mobile devices and IT assets Arrow bylined article published in China and Thailand An article written by Arrow Value Recovery's Scott Venhaus has been published in China and Thailand. As Arrow's Value Recovery business has now expanded to China, this timely article raises awareness among IT practitioners…Continue Reading →
May 4, 2016 – We are pleased to announce the opening of a new Arrow Value Recovery sales office in Shenzhen, China. The establishment of this office supports Arrow's growing client base with local sales representation and service support. This announcement builds Arrow Value Recovery's growth in Asia as it follows on the heels of…Continue Reading →
On Earth Day, April 22, 2016, 175 countries signed the Paris climate accord. Literally decades of work led to this historic moment. Now what? The climate goal will be translated into goals set by countries and, within those countries, goals set by governments and corporations in what needs be the ultimate call for collaboration. But…Continue Reading →
Two weeks ago in Paris, EcoVadis held its annual conference, providing content to both its customers and its global staff of analysts, those ultimately responsible for scoring companies across a spectrum of corporate social responsibility and sustainability criteria. The content ranged from the macro – current trends as presented in the opening keynote by author…Continue Reading →
Find Cracks in the Corporate Armor to Improve Sustainability and Bottom Line. There seem to be, in my overly simplistic view of the world, two kinds of companies: – companies that use their sustainability values to inform their business practices, and – those who resist or don't recognize the s-word. But regardless of their external stance…Continue Reading →


text:
Star Tracks
Richie works her bouffant at the FiFi Awards in N.Y.C. Plus: Katie & Suri, Brad Pitt, Anne Hathaway and more
Credit: Sharpshooter Images/Splash News Online
Updated: Tuesday May 22, 2012 | 06:00 AM EDT
More from PEOPLE
Latest News!
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Positivt för månatlig rapportering
Allt tyder på att vi från den 1 januari 2018 kommer att ersätta årliga kontrolluppgifter med...
Srf konsulterna, former Sveriges Redovisningskonsulters Förbund, SRF, is the association of Swedish Accounting and Payroll consultants and was formed in 1936. Srf konsulterna has 3 500 Authorized Accountants and Payroll consultants as active members all over Sweden and a total of 5 500 members, engaged by 330 000 companies, both Swedish and international.
We demand that all our consultants are highly qualified, committed to quality in their work as well as their own personal development. A consultant's competence is thoroughly screened during the recruitment stage.
We set the bar high for our members and our ethics are underpinned by the rigorous pursuit of quality. We also make sure that our members update their knowledge on a regular basis. This is crucial in the accountant's and payroll consultant's role as company advisor and partner.
An Authorized Accountant must follow the Swedish standard of accountancy, called Reko. He or she must also undergo regular quality checks made by Srf every six years. The Authorization is a stamp of approval from Srf and warrants the accountant's high level of competence and experience.
Our goal is that a consultant approved by the organisation is the natural option for any company or enterprise.
We carefully monitor the latest trends in finance, payroll and accounting, and we play an active role in government legislation through close co-operation with government authorities and other organisations.
Education and training is very important to us as we strive towards continual growth and development. We offer a wide range of courses, and through this, we are able to ensure that our members and professionals remain at the forefront, which in turn reinforces our customers' trust in us.
Find an Authorized Consultant in Sweden
Ditt företags debiterade F- eller SA-skatt ska vara bokförd på Skatteverkets konto.Läs mer
Allt tyder på att vi från den 1 januari 2018 kommer att ersätta årliga kontrolluppgifter med...
Nu kan du boka dig till årets inspirerande Srf dagar och den stora jubileumsfesten. Det har väl inte undgått dig...
Ett nytt samarbete har under april månad inletts mellan Srf konsulternas förbund och Småföretagarnas...


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FOR SALE - Nest of 3 inlaid tablesAberdare £45.00
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Nest of 3 light oak finish tables with veneer inlay and rolled legs. Mark free and quite eye catching.
Ad ID: 97151491


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The Fritz Castle attraction, secretly considered to be the best ethereal attraction at Disneyland
Deep within Fritz Castle is a twisting dungeon corridor decorated with holograms of characters from the Fritz animated feature. Be on the lookout for dragons and even more thrills!
We suggest you don't try Zombie Mode
© copyright 2011–2016
A Pants Aflame production


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Let's say I have this HTML:

Fozzy Bear

Steve Martin

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... and then an event listener is added with jQuery:
$('ul li').click(function() { console.log('waka_waka!!!'); });
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Are those event listeners automatically detached and released? Or will this actually cause a leak of memory? Is it best practice for one to remove those listeners prior to removing?


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X-Men: Apocalypse
X-Men: Apocalypse is a confused, bloated, mess of a film.
X-Men: Apocalypse is a confused, bloated, mess of a film.
As its title suggests, Unlocking the Cage is a kind of advocacy journalism, not an attempt to weigh the cons as well as the pros…
"The Ballad of Narayama" is a Japanese film of great beauty and elegant artifice, telling a story of startling cruelty. What a space it opens…
Patrice Leconte's "Monsieur Hire" is a tragedy about loneliness and erotomania, told about two solitary people who have nothing else in common. It involves a…
Ken Loach's "I, Daniel Blake" wins the Palme d'Or at the 69th Cannes Film Festival.
Barbara Scharres on the cats of Cannes 2016.
Roger's Favorites: actress Faye Dunaway.
When I began as a film critic, Jean-Luc Godard was widely thought to have reinvented the cinema with "Breathless" (1960). Now he is almost 80…
Part two of Jana Monji's essay about the portrayal of Asian characters in cinema.
Part one of Jana Monji's essay about the portrayal of Asian characters in cinema.
The success of shows like "Inside Amy Schumer" and "Broad City" has opened the door for more shows with female comedians front and center, including…
A set report from the most expensive film yet made in Romania, "Octave."
Urban legends tap our deepest fears, and one of the most subterranean involves the call for help that is laughed at or ignored. We cry out again and again, only to be dismissed by our friends, or the 911 operator, or strangers on the shore. At the beginning of Bernard Rose's "Candyman," we hear an urban legend about a woman in a high-rise public housing project, who calls for 911 but is not taken seriously. Not long after, her body is found, savagely slashed to death. The Candyman has struck again.
Who is the Candyman? According to the movie, he is a powerful supernatural being who haunts Cabrini-Green, the housing complex on Chicago's Near North Side. He lures victims with candy, or puts razor blades in Hollywood treats - the details are vague and dreamlike - and his lair is an abandoned apartment on one of Cabrini's upper floors.
All of this information is carefully written down by two researchers from the University of Illinois (Virginia Madsen and Kasi Lemmons), as part of a research project that also touches on such matters as alligators in sewers. Madsen, trapped in an unhappy marriage with a philandering professor, throws herself into her work, dragging Lemmons along as they interview the neighbors of the Candyman's latest victim. Oddly enough, their stories seem to support the legend - even though the theory is that these urban tales never quite check out.
Rose is a director who likes stories about supernatural invasions of real life. His brilliant "Paperhouse" (1989), about a young girl whose drawings seemed to influence the life of a boy in her feverish dreams, used images of razor-sharp reality to suggest that the dreams were as real as the rest of the movie. "Candyman," from Rose's own screenplay, based on a Clive Barker story, does the same thing. We think we'll discover that the Candyman is actually a real, live human being - a killer using the legend as a cover. What we do discover is more frightening, and more intriguing. He may literally be a product of the imagination.
What if urban legends became real if enough people believed in them? What if the sheer psychic weight of faith from thousands of people were enough to create a supernatural reality? If everyone believed there were alligators in the sewers, would there be? Are gods the result of man's faith in them? Would the Candyman therefore take a dim view of a researcher's attempts to debunk him? Madsen and Lemmons, courageous and plucky, make sympathetic heroines as they walk up and down the dangerous stairwells of Cabrini-Green, crawling through empty apartments looking for a monster. Rose has been clever in his use of locations. Just as urban legends are based on the real fears of those who believe in them, so are certain urban locations able to embody fear. Empty apartments in the upper floors of public housing projects are, it is widely believed, occupied by gangs. We perceive a real threat to the women, at the same time they're searching for what they think is an imaginary one.
Then the plot thickens. Rose evokes Hitchcock's favorite formula, the Innocent Victim Wrongly Accused. Just as the Candyman's victim called 911 and was not believed, so Madsen is arrested by the police and her story scornfully dismissed. It's all kind of intriguing. Elements of the plot may not hold up in the clear light of day, but that didn't bother me much. What I liked was a horror movie that was scaring me with ideas and gore, instead of simply with gore.
Separating the artist from the art isn't as easy as it sounds.
This message came to me from a reader named Peter Svensland. He and a fr...
Part two of Jana Monji's essay about the portrayal of Asian characters in cinema.


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Starting small and going wild
Incoming beer company has unique business plan
Ian Anderson 5 p.m., May 30
Posted January 18, 2014
Author: Salvatore Scafidi Neighborhood: Mission Hills Age: 59 Occupation: Retired Engineer Ethel's voice cuts through the hubbub of the grandkids' wrestling over the Etch-a-Sketch and Dr. Phil's scolding of another drug-soaked teenager. Fred blinks and ...
Ian Anderson 5 p.m., May 30
Daniel Powell 4:23 p.m., May 30
Robert Duffy 1 p.m., May 30
Walter Mencken 12:02 p.m., May 30
Don Bauder 11:30 a.m., May 30
Ian Anderson 11 a.m., May 30
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Iggy Pop. Iggy Pop. Wallpaper and background images in the Annie Leibovitz club tagged: iggy pop annie leibowitz famous photograph famous photographer.
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New Products
New REESE'S Snack Mix from Hershey's
Hershey's introduces a new line of Sweet and Salty Mix with REESE'S Peanut Butter Cup Minis and REESE'S PIECES Candy, Pretzels, and Peanuts. The hunt for the perfect snack has come to an end! With its pantry-perfect packaging, the REESE'S Snack Mix are perfect for on-the-go and everyday snacking. This variety snack is available in Read More > $gt;
New Kid Zbar Fruit + Veggie Bars from Clif Bar & Co.
Clif Bar & Co.'s Clif Kid division recently introduced Clif Kid Zbar Fruit + Veggie. Made with whole grains, vegetable powders and a blend of fruit puree, these certified U.S.D.A. organic snack bars contain no bioengineered ingredients, high-fructose corn syrup, artificial flavors or synthetic preservatives. The chewy bars are available in three flavors: Awesome Orange, Read More > $gt;
Manischewitz® Partners with Welch's® To Bring Welch's Manischewitz 100% Grape Juice to Consumers
Newark, NJ…Have you ever taken a bite of a fresh concord grape? Feel the rush of the sweet juice, as that wholesome grape flavor fills your mouth? Manischewitz®, the leader and pioneer in kosher products, is proud to announce a special partnership with Welch's®Grape Juice Brand, the nation's largest grape juice producer. Manischewitz and Welch's Read More > $gt;
New Flavored Honey from Tu-Bees Foods
Honey creamed with natural flavors and using only the finest of ingredients to create a product so delectable that it will make your taste buds start buzzing with happiness. Tu-Bees Foods is a Canadian honey business that specializes in gourmet flavored creamed honey products. All Tu-Bees products contain unpasteurized creamed honey combined with all natural Read More > $gt;
Manischewitz® Partners with Welch's® To Bring Welch's Manischewitz Sparkling Grape Juice
Newark, NJ – Manischewitz®, is proud to announce a special partnership with Welch's®, Grape Juice Brand. Manischewitz and Welch's have teamed up to offer consumers delicious non-alcoholic, Sparkling Grape Juice in a 25.4oz bottle that has no artificial flavors, no artificial colors and is sulfite free. "With Welch's expertise in Grape Juice combined with Manischewitz leadership in Read More > $gt;
More than 300 New Items to hit Passover Shelves in 2016
New York – With Passover now two months away (begins eve of April 22nd), the special holiday shelves in grocery aisles will soon come to life and this year like in previous years, consumers will find more than 300 new items to choose from. While there are no "revolutionary" new Passover items like pizza, bread crumbs and Read More > $gt;
New Parmesan Cheese Shaker from Tnuva
Expanding upon its offerings of quality cheeses, Tnuva recently unveiled its new grated Parmesan Cheese in an easy-to-use shaker. The dry grated classic parmesan cheese is strictly supervised from the farm to the final packaging by the New Square Rabbinical Kashrus Council, the Orthodox Union and the united Mehadrin hechsherim under the Va'ad Mehadrin Council. Read More > $gt;
Tribes-A-Dozen New Hallah Egg Bread Mixes
Washington, D.C – Tribes-A-Dozen recently expanded distribution of its all-natural, kosher, under the Orthodox Union (OU) line of Voilà! Hallah Egg Bread Mixes to the kosher shelf of conventional and specialty markets in the Midwest – Meijer; South – Publix; and the Chicago area and Madison, WI — Roundy's Mariano's and Copps, respectively. This is Read More > $gt;


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Mass Effect Trilogy (and more!) added to Origin Access Vault
Still haven;t played Mass Effect? Now you've got no excuse! The trilogy of games is available as part of Origin Access.
Titanfall 2 may feature grappling hooks
Accrding to anew info leak, Titanfall 2 will feature larger maps, more players and a grappling hook.
Here's Mirror's Edge: Catalyst's launch trailer
It wasn't quite everyone's cup of tea, Mirror's Edge. While I loved the vibrant and colourful first person parkour platforming; the point of view, perspective and pace made many want to vomit in to their own red shoes
Plants Vs. Zombies: Garden Warfare 2 Trouble in Zombopolis: Part One announced
Prepare for a new herbal assault, as Plants Vs Zombies: Garden Warfare 2 gets a big upgrade with the free Trouble in Zombopolis DLC.
More Yarny? EA and Unravel maker extend partnership
Unravel's reveal at E3 was the most authentic part of EA's lineup, and they're going to keep that going in the future.
Battlefront skipped its single player to capitalise on Force Awakens fervour
Star Wars: Battlefront didn't have a single player campaign so that EA could capitalise on fan excitement over Episode VII – but it's a mistake that's unlikely to happen again.
BioWare's new IP appears to be an action game
EA's BioWare has a new IP in the words, and it's looking like it will be an action game.
Battlefield 1 is "right for the franchise and right for EA"
EA Studios boss Patrick Soderlund initially rejected the World War One pitch for Battlefield 1, but DICE managed to win him over in the end.
Battlefield 1's debut trailer is pretty darn historically accurate
Battlefield 1 is really digging deep into the history books if its first trailer is any indication.
Battlefield 1 wants to prove that World War 1 wasn't actually boring
Battlefield 1 wants to prove that the World's First War wasn't as boring as hostory makes it out to be
EA isn't worried about launching Titanfall 2 and Battlefield 1 in the same period
EA is launching Titanfall 2 and Battlefield 1 in the same quarter. They don't think it's a silly idea launching two big shooters in the same quarter.
Why Call of Duty's priced right – and you can expect other games to follow suit
Looking back at the pricing gap between Call of Duty: Infinite Warfare and Battlefield 1 – it seems that


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Job Openings at St. Louise School
Please note the positions for which we're currently accepting applications.
Dedicated to providing quality education for pre-kindergarten through eighth grade.
Find More Information about the Annual School Gift Campaign
Please note the positions for which we're currently accepting applications.
Dan's Blog talks about an exciting week of camp for our 6th graders at a new location.
Three Distinguished Grads were announced for 2016 and they are all in the same family!
Save the Date!
Congratulations Oluchi Anunobi, St. Louise Class of 2013, for being named Student of the Month in NW Catholic Magazine for May.
Kindergarten Concert
Date: 5/25/2016, 2 PM – 2:30 PM
Gr. 8 Service & BBQ
Date: 5/27/2016
No School Only for Grade 6
Date: 5/27/2016
*NO SCHOOL* (Kids' Club Closed)
Date: 5/30/2016
Memorial Day
Date: 5/30/2016
|Show Calendar »
|Show All »


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It was alright, but did you get permission to "borrow" the idea of Killbox?
it was alright but i didnt like the credits durin the movie 2/5
Cool, for a stick man movie!
I think the way your names kept appearing was deadly annoying, but it was better than alot of stick man movies, remove the names and it'll be great! P.S. Freewebs sucks!
its longer than a normal stick movie so thats why i dont blame its
also the last stick was good animated.
i hope it wont get blammed for you.
ps. music is good
thx i also like the music, and thx for the good review
Its not the best buts its good
hey its not great but its better than what i could do but hey its funny and enjoyable so gw
thx i wil look foward to seeing your movies and voting 5