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Is there such a thing as life after death?
While this was once a question pondered by philosophers and religious groups, scientists are now seriously considering the possibility that consciousness can continue after a person has been clinically diagnosed as dead.
A large-scale study involving 2,060 patients from 15 hospitals in the UK, USA and Austria has found that patients experience real events for up to a three-minute period after their heart has stopped beating.
Dr Sam Parnia, assistant professor of critical care medicine and director of resuscitation research at the State University of New York and a former research fellow at the University of Southampton, said it was previously thought only hallucinatory events were experienced in these circumstances.
These are normally described as out-of-body experiences (OBEs) or near-death experiences (NDEs).
The Awareness during Resuscitation (Aware) study, sponsored by the University of Southampton, used objective markers to establish whether the experiences were real or hallucinatory.
The results showed that 39% of patients who survived cardiac arrest described a perception of awareness but did not have explicit recall.
A total of 46% experienced a broad range of mental recollections, 9% had experiences compatible with NDEs and 2% exhibited full awareness compatible with OBEs with explicit recall of "seeing" and "hearing" events.
One man in particular, a 57-year-old social worker from Southampton, was reported to have given a "very credible" account of what was happening in the room during the three minutes that he was "clinically dead".
The man could describe the movements of the doctors and nurses in the room with uncanny accuracy. He told the researchers he felt he was standing in the corner of the room, observing the medical staff resuscitate him.
Dr Parnia said: "This is significant, since it has often been assumed that experiences in relation to death are likely hallucinations or illusions, occurring either before the heart stops or after the heart has been successfully restarted, but not an experience corresponding with 'real' events when the heart isn't beating.
"In this case, consciousness and awareness appeared to occur during a three-minute period when there was no heartbeat.
"This is paradoxical, since the brain typically ceases functioning within 20-30 seconds of the heart stopping and doesn't resume again until the heart has been restarted.
"Furthermore, the detailed recollections of visual awareness in this case were consistent with verified events.
"Thus, while it was not possible to absolutely prove the reality or meaning of patients' experiences and claims of awareness, (due to the very low incidence - 2% - of explicit recall of visual awareness or so called OBEs), it was impossible to disclaim them either and more work is needed in this area.
"Clearly, the recalled experience surrounding death now merits further genuine investigation without prejudice."
Story continues below slideshow...
The actress has described her near-death experience on several occasions, including during an interview with Larry King in 2005. An allergic reaction caused Seymour to go into anaphylactic shock. Though she told King she doesn't belong to a specific religion, Seymour said she saw light and believes "there is some spiritual entity that's greater than us." In another interview with BeliefNet, Seymour said she lives life to its fullest and tries her best to make a difference.
Since his own near-death experience in 2008, during which he was virtually brain dead for a week, academic neurosurgeon Eben Alexander has been studying the relationship between science and spirituality, the results of which are set to be published in a 2012 book. "I do not believe that there is a good neuro-physiologic explanation for what happened to me," said Alexander on an episode of "Through the Wormhole with Morgan Freeman."
Clinical psychologist Mary Jo Rapini appeared on "TODAY" in 2010 to talk about the near-death experience she had following a brain aneurysm. She is only one of the more than 1,000 patients studied by Dr. Long. In a YouTube video, Rapini said God -- who was more of a voice and had no real face or shape -- said to her: "Have you loved any person the way you've been loved in this short time here? ... You can do better."
In 1991, singer-songwriter Reynolds underwent hypothermic cardiac surgery. Once the surgery was complete, Reynolds woke and described the near death, out-of-body experience she had while unconscious. According to articles on the subject, including one published in Progress in Brain Research in 2005, Reynolds said she watched the surgery from outside her body, listened to music playing in the operating room and met with her deceased relatives. Medical professionals have argued over exactly what happened to Reynolds, such as on a 2009 National Public Radio segment. Cardiologist Michael Sabom, M.D., who researched near-death experiences (including Reynolds'), discusses her case in his book "Light and Death." Reynolds passed away in 2010.
Though he didn't have a near-death experience himself, Lommel spent significant time researching the topic, the results of which appeared in a 2001 paper published in The Lancet. On his website, Lommel describes some of the typical experiences of his research subjects. A 44-year-old man, for instance, described accurately everything that happened to him while he was brought to the hospital comatose. Others reported a disappearance of the fear of death, seeing their entire life in perspective and knowing "the thoughts of everyone involved in the event, as if I had their thoughts within me" and encounters with deceased relatives.
Research psychologist at Nottingham Trent University Dr David Wilde, is currently gathering data on out-of-body experiences in an attempt to discover a pattern.
Speaking to The Telegraph, he said: "Most studies look retrospectively, 10 or 20 years ago, but the researchers went out looking for examples and used a really large sample size, so this gives the work a lot of validity.
"There is some very good evidence here that these experiences are actually happening after people have medically died.
"We just don't know what is going on. We are still very much in the dark about what happens when you die and hopefully this study will help shine a scientific lens onto that."
The study was published in the journal Resuscitation.
Scientists Find Hints for the Immortality of the Soul
Near-Death Experiences May Not Be Evidence Of An Afterlife, Rather A Surge In Brain Activity


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CSI: Crime Scene Investigation 2000 | TV Show Watchlist
Season 12, Episode 4 Maid Man
First Aired: October 12, 2011
A former mayor of Las Vegas is shot at the Mob Museum and the CSIs embark on a Vegas history lesson to find clues to the crime.
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Phillip Rhys Phillip Rhys Prince Hamad Al Maktoum
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Monique Gabriela Curnen Monique Gabriela Curnen Xiomara Garcia
Frances Fisher Joanna Sapphire
Vincent Corazza Vincent Corazza Stanley Gant
Barbara Eve Harris Sheriff Sherry Linson
Sheeri Rappaport Sheeri Rappaport Mandy Webster
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subset: commoncrawl


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Victoria s firefighting capabilities have received a boost with the Metropolitan Fire Brigade unveiling its new Fireboat 1, Acting Premier Peter Ryan said today.
What does MFB stand for?
MFB stands for Metropolitan Fire Brigade (Australian non-volunteer)
This definition appears frequently and is found in the following Acronym Finder categories:
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- Maryland Farm Bureau
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- Mittelstand und Freiberufler (category of small businesses in Austria)
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Samples in periodicals archive:
Melbourne, July 23 (ANI): The metropolitan fire brigade in Melbourne has urged men to be more careful while dealing with stainless steel washers and rings, and not risk getting them stuck on their penises.
ESTA is responsible for dispatching calls for the following agencies: Country Fire Authority (CFA); Metropolitan Ambulance VictoriaService (AV); Metropolitan Fire Brigade (MFB); Victoria Police (VicPol); and, Victoria State Emergency Service (VICSES).
We mention this because it gives us a chance to pass on the following passage from the Melbourne Age newspaper, who report that a Metropolitan Fire Brigade spokesman said 18 firefighters "were tied up for some time, but disciplined and controlled firefighting contained the blaze to one room on the second floor".
Last night the Office of the Deputy Prime Minister, which sets the grant, said compared to other metropolitan fire brigades, Tyne and Wear's grant was "not below average".
It is supported by a number of private businesses and organisations including Nexus, Marks & Spencer, Woolworths, Northern Electric, Tyne and Wear Metropolitan Fire Brigade and Northumbria Police.
I took command of the West Midlands Fire Service, the second largest Metropolitan Fire Brigade in the country early in 1998, having served over 30 years in the Fire Service in other parts of the country.
Metropolitan fire brigades will receive slightly less - an average of 4.


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Throwback Thursday: Hellcat Challenger Battles Viper on the ½ Mile
This week's Throwback Thursday video features a series of head to head battles between the newest Dodge Viper and the mighty Dodge Challenger SRT Hellcat. According to the details, both of these cars are factory stock, so the Challenger has 707 horsepower and 650lb-ft of torque while the Viper has 640/645hp (depending on the model […] More »
Hellcat Challengers and a Charger Make High Speed Blasts
We have featured plenty of Hellcat Challengers and Chargers in action on the quarter mile, but today we bring you a look at a handful of the supercharged Mopar muscle cars taking on the longer tracks – and laying down big speed numbers. Below, I have attached four videos – three showing a Dodge Challenger […] More »
2016 Roadkill Nights by Dodge Coming to Kansas City, Detroit
The Chrysler Group has announced that there will be a 2016 Roadkill Nights by Dodge, following up on the successful 2015 campaign with a pair of events – one in Kansas City and another in Detroit. Last August, Dodge teamed up with the crew from the popular internet series Roadkill to introduce Roadkill Nights by […] More »
Worlds Quickest Manual Hellcat and a Hot Girl
When the Chrysler Group rolled out the Dodge Challenger SRT Hellcat, they should have done so in a similar fashion to the video below – with dramatic music, burnouts and a very hot blonde model. This white 2015 Dodge Challenger SRT Hellcat might look familiar, as it is one that we have featured before here […] More »
Ride Along with Justin Bell in the Viper ACR
While attending the SRT Experience at Bondurant Racing School a few weeks back, I had a chance to climb aboard the new Dodge Viper American Club Racer with endurance racing champion Justin Bell. If the name Justin Bell doesn't ring a bell, shame on you. Bell was one of the drivers who helped pilot the […] More »


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Millennials Not Thrilled with their Bank or its Services
Millennials would rather use their mobile phone than do their banking at a traditional branch by an overwhelming margin, according to a new survey from Chime, a mobile banking company. The research showed 88% of Millennials would rather use a bank app or website than perform a banking service at a branch. The country's largest living
Millennials Not Thrilled with their Bank or its Services
October 13, 2015
Apple Pay to Expand to Starbucks, KFC and Chili's
Dow Jones the Latest Victim of Data Breach
October 12, 2015
Bank Fees Continue to Rise
Cash Becoming Obsolete as Digital and Contactless Payments Increase
Chase Slate vs. Citi Double Cash
LowCards.com Weekly Credit Card Update–October 9, 2015
October 9, 2015
Companies Not Doing Enough to Protect Consumer Information
October 8, 2015


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Integrating Google AdWords and Google Analytics makes for a data rich experience. You can use this data to go into a second level of PPC advertising analysis beyond the impressions, clicks, conversions, etc., found in AdWords to see more deeply how campaigns are impact other media and paid search user behavior.
Here are three examples of how combining Google AdWords and Analytics can help improve the performance of your paid search campaigns.
1. End to End Paid Search Report
This custom report for Google Analytics contains all the goodies needed to get a great snapshot of paid search in AdWords. It includes info like percentage of new visits and revenue per click, and ties into Google Analytics goals and ecommerce tracking.
Use this data to view reporting with multiple goals and revenue reporting for ecommerce tracking. Customize the paid search report to each unique account by adding different metric groups or filters. Google Analytics offers a wider variety of data than AdWords can offer alone.
Download and read more in the Google Analytics Solutions Gallery.
2. Multi-Channel Funnels Reporting With Top Conversion Paths
Google Analytics will give credit to conversions and ecommerce transactions that occurred via last click or last campaign, search, or ad that referred the visitor when he or she converted.
For most searchers, there are multiple contact points with other advertising, searches, or websites. This report will show how different marketing channels contribute to the conversion path. The conversion path will include data from paid search, but also organic, email, direct visits, and more
You can find it in Google Analytics in the left navigation under Conversions > Multi-channel Funnels > Top Conversion Paths
Use this data for paid search to see how PPC is contributing to the overall channel mix for sales.
In the reporting example above, we can see in #4 that two transactions were made by directly going to the website, but they initially clicked on a paid search ad that led them to that end.
3. Matched Search Queries
In the left navigation under Acquisition > AdWords > Matched Search Queries, you will find a list of actual search queries that triggered impressions of your AdWords ads. Adding a secondary dimension takes the keyword query report to the next level.
For example, combine query data with behavior, conversions, social, and more. To analyze user behavior and how the user interacts with the website, examine the bounce rate, pages per visit, and average visit duration, which all speak to the engagement of the user with the content. Next, conversion columns show how goals set in Google Analytics were reached.
Using Visitors data in the secondary dimension opens to the door to in-depth information including demographic like age, gender, and location (must have demographic reporting enabled.) Advertisers can use this information, for example, to build personas or optimize targeting in AdWords.
There are endless possibilities to use Google Analytics data to gain insights and optimize the performance of AdWords PPC. These three examples are a great starting point to dig and start improving results today.
What's your favorite way to use Google AdWords and Google Analytics together?
Related reading
Tie your web, and mobile properties together in Search Console, plus three more recent changes
Since writing our comprehensive and barn-stormingly popular complete guide to Search Console a couple of weeks ago, Google has since released a few updates to make webmasters lives a darn sight easier.
Google launches Firebase Analytics for mobile apps
Google has launched Firebase Analytics, a new analytics solution for mobile apps, at this year's I/O 2016 developer conference.
Google Search Console: a complete overview
The Search Console (or Google Webmaster Tools as it used to be known) is a completely free and indispensably useful service offered by Google to all webmasters to help optimise their sites for search.
Do bounce rates affect a site's search engine ranking?
Bounce rates and how they affect a website's ranking on Google has been discussed, dissected, and dismembered over and over again.


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Ghost Billboard On U.S.- Canadian Border
Created by an art studio called Lead Pencil Studio on a U.S. government commission. The billboard stands on the U.S./Canada border and is intended to highlight the proliferation of signage on that part of the border. More info here.
This post was created by a user and has not been vetted or endorsed by BuzzFeed's editorial staff. BuzzFeed Community is a place where anyone can post awesome lists and creations. Learn more or post your buzz!
- Protests outside a Donald Trump rally in New Mexico turned violent Tuesday night as demonstrators threw rocks and bottles at police officers.
- The Afghan Taliban has picked an extremist scholar as its successor to leader Mullah Mansour, who was killed in a U.S. drone strike last week.
- Twitter will no longer count links, @names, and GIFs toward its 140-character limit. You can also retweet yourself now.


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I publish these three novenas which I have recited for nine days.
O most holy apostle, St. Jude, faithful servant and friend of Jesus -- People honor and invoke you universally, as the patron of hopeless cases, of things almost despaired of. Pray for me, for I am so helpless and alone. Please help to bring me visible and speedy assistance. Come to my assistance in this great need that I may receive the consolation and help of heaven in all my necessities, tribulations, and sufferings, particularly (I ask that you make Chris change his mind and come back to me so we can be together as a couple again.) and that I may praise God with you always. I promise, O blessed St. Jude, to be ever mindful of this great favor, to always honor you as my special and powerful patron, and to gratefully encourage devotion to you by publishing this request on the ninth day. Amen.
Novena to St. Anthony of Padua
O wonderful St. Anthony,
glorious on account of the fame of your miracles,
and through the condescension of Jesus
in coming in the form of a little child to rest in your arms,
obtain for me of His bounty the grace
which I ardently desire from the depths of my heart...
(I ask that you make Chris change his mind and come back to me so we can be together as a couple again)
You who were so compassionate toward miserable sinners,
regard not the unworthiness of those who pray to you,
but the glory of God that it may once again be magnified
by the granting of the particular request...
(I ask that you make Chris change his mind and come back to me so we can be together as a couple again)
which I now ask for with persevering earnestness.
Novena to St. Claire
Ask for 3 favors 1 business, 2 impossible. Promise to publish or circulate. Your request will be granted after the 3rd day. Never known to fail. Say 9 Hail Mary's and the following prayer for 9 days in front of a lighted candle.
1. I ask to find a new, better paying job
2. I ask that you make Chris change his mind and come back to me so we can be together as a couple again
3 I ask for good health for my parents.
God of mercy you inspired St. Claire with the love of poverty by the help of her prayers. May we follow Christ in poverty of spirit and come to the joyful vision of your glory in the kingdom of heaven.We ask this thru our Lord Jesus Christ your Son, Who lives and reigns with the Holy Spirit, One God forever and ever.Amen. Recite 9 Hail Marys
Published Novena
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Having 1 admirer is not alot.. do we really need to be making a thread about him and how he is still in the friend zone? Just remember before you hit create topic, LOL @ your 1 admirer!
I have been playing Fallout 4 but i got bored of it because i was expecting something like New Vegas , filled with lots of quest not just an open world with nothing I wan to play Mas effect Andromeda, Kingdom come deliverance , Mount and blade 2
Went out to a lounge to meet a female friend -Sat next to her and next to me was this Libra -My female friend and I were conversing -Libra kindly found his way into the conversation -I asked him his name after a while and introduced him to my female f
Went out to a lounge to meet a female friend -Sat next to her and next to me was this Libra -My female friend and I were conversing -Libra kindly found his way into the conversation -I asked him his name after a while and introduced him to my female f
Do you guys remember this cool little device? You'd put the tiny cassette thing into the thingy and it would play 30 seconds of the song. OH MY GOD MY CHILDHOOD. your 90's things now. xoxox
I have a relationship with a gemini man. And one day we have a fight. And I don't know how, he turns my words against me, and turn me into a bad guy. He texted me: "I don't want to think & fight right now." Then I replied: "If you don't want to think &
Right now I hate myself and my life for not being able to leave a woman who fucking doesnot even love me anymore. Bitch has no respect no fucking boundaries .... These are type of woman who will led men to cheat,,,,,,,,
Thoughts on this??? I agree with most of it, especially; Never assume that the person you are dealing with is weaker or less important than you are. Some people are slow to take offense, which may make you misjudge the thickness of their skin, and
So I'm talking to this guy Who's born Dec 15 that's all I know so far ... We instantly clicked .. I'm a Taurus and I don't usually get along with sag men but I'm willing to try this guy out he seems so much more mature then the other sags I've talked to .
Can anyone tell me how to be more Venusian? How do I activate my Venus? I've read that this placement is supposed to make you charming but I'm not seeing it.
Sometimes I feel like I had it, but then if I did, I probably would be dead by now. They say they usually don't make it to 20. The only time I've seen it was in that movie called "Jack" but that shit was kind of extreme lol.
...if you have pets and are trying to rent.
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PC U.S.A.'s Ban on Gay Clergy Ends Sunday
PC U.S.A.'s Ban on Gay Clergy Ends Sunday
PC U.S.A.'s Ban on Gay Clergy Ends Sunday
Presbyterian USA Close to Allowing Gay Clergy
Oklahoma lawmakers have approved a bill that gives church leaders the right to object to performing same-sex marriage ceremonies.
The Presbyterian Church USA has voted to change the definition of marriage to include gay marriage.
When Dann and Tracey's car was hit head-on by a drunk driver it burst into flames. With their lives...
700 Club Canada's Laura-Lynn Tyler Thompson shares about her experiences with brokenness. Plus, while Lee's parents were preoccupied with...
Scott can't believe his own success story. He and his wife struggled to survive on welfare for years. Scott...
Author John Eldredge shares his experience of the epic motorcycle adventure he took with his sons.


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Are You a Nail Polish Commitment-Phobe? Then These Polishes Are For You
Switching your nail polish as often as you change your clothes might seem like a tempting idea, but the whole process can discourage even the most git-'r-dun beauty junkies. That's why we love the idea of nail polishes that keep up with our whims, from top coats that you can layer over a lackluster manicure to polishes that peel off when you're ready to say goodbye. Here are our favorite nail polishes for the commitment-phobic.
Sula Paint & Peel Duos in Azure and Watermelon, $9, available at Sulabeauty—For the ultimate in quick-change options, try Sula's Paint & Peel Duos. The water-based polish doesn't smell and literally peels off—no remover necessary.
Nubar 2010, $7.49, available at Bynubar—Switch it up instantly by adding an otherworldly, iridescent effect to any shade with Nubar's sheer 2010 polish with reflective flakes. OPI Black Shatter, $9, available in stores in January—Paint this topcoat over any base color, and the polish will quickly dry in sections, creating a shattered effect that almost looks like lizard skin.
Orly Plastix Collection in Viridian Vinyl, Purple Pleather, Old School Orange, and Retro Red, $7.50 each, visit Orlybeauty.com for locations—One second you want matte, the next, shine. Why not go for both? Orly's Plastix collection creates a pleather-like finish that gives a rubbery, latexy look that fits in between full-on shine and flat-out matte.
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Tomasi, Gleason Talk the Death of Superman, "Truth, Justice & Family" in Rebirth
Comic Books
There's only one thing that could get me to miss FCBD today and it's LA Clippers Playoff Basketball! Here are the Avengers at LA Live across from Staples Center. Someone should have set up an FCBD booth down here.


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Travel Counsellors is a leading independent travel company with one of the highest customer service (NPS) scores in the world and a turnover at the end of its last financial year (Oct 31 2012) of £360 million. Founded in 1994 it currently has over 1,200 t ... more
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A modernised and charming, two bedroom stone built cottage. Situated in the popular village of Henllan, surrounded by beautiful countryside yet only a short 2.5 mile drive into Denbigh Town Centre where there is a vast amount of amenities from local shops, butchers, restaurants, schools and colla...
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The Alfried Krupp Fellows Programme
Each year the Alfried Krupp Wissenschaftskolleg awards 3 to 6 Alfried Krupp Senior Fellowships and 6 to 12 Alfried Krupp Junior Fellowships. The Institute enables the fellows to concentrate on a major academic project, free of extensive teaching duties. The Institute also offers the opportunity to discuss their discipline with colleagues and students at the Ernst Moritz Arndt University of Greifswald.
Academics who have proven themselves by outstanding research and teaching are appointed as Alfried Krupp Senior Fellows.
Alfried Krupp Junior Fellowships are awarded to particularly qualified young academics with doctorates.
A joint application by several people who wish to realize a project in Greifswald in interdisciplinary and international cooperation is possible.
Alfried Krupp Fellowships are awarded either for a semester (1st October to 31st March or 1st April to 30th September) or for a whole academic year. They may be awarded to academics from Germany or from abroad.
Alfried Krupp Senior Fellowships and Alfried Krupp Junior Fellowships are advertised publicly. Proposals and applications should be submitted to the academic director by the deadline stated in the respective advertisement.
Applicants and persons who wish to propose a researcher to the academic director of the Institute are requested to observer the guidelines for applicants and on submitting proposals, which can also be downloaded as a PDF file.
Where possible, Alfried Krupp Senior Fellows should arrange to be substituted at their respective home institution by a junior academic. The substitution costs will be borne by the Institute. Furthermore, Alfried Krupp Senior Fellows are provided with an apartment and an office, free of charge, in the Alfried Krupp Wissenschaftskolleg. Individual offers may be made by the Institute to Alfried Krupp Senior Fellows who cannot be granted paid leave by their home institutions.
The fellowships are funded according to the high expectations concerning the scientific achievements of the fellows. As a rule the grant is tax-free in Germany. Additionally, Alfried Krupp Junior Fellows are provided with an apartment and an office, free of charge, in the Alfried Krupp Wissenschaftskolleg.
Travelling and laboratory costs are not covered by the Institute.
During their stay Alfried Krupp Fellows are expected to complete, or at least to make decisive progress in, a significant research project.
Fellows are expected to live in the Institute.
The Alfried Krupp Wissenschaftskolleg would welcome it if Alfried Krupp Senior Fellows were to hold a class for the students of Greifswald University during their stay.
Further information for applicants and on submitting proposals may be found here.
29/05/16 Russland im Fokus
Russisches Filmforum im Rahmen der Russischen Kulturtage
30/05/16 baltisch
Musikalische Einführung in die 70. Greifswalder Bachwoche in Kooperation mit der Gesellschaft zur...


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Chartered Membership (CMILT) and International Advanced Diploma in Logistics and Transport will be one of the major programmes offered by CILT International at Wisdom Business School FZE.
What does Adv Dip stand for?
Adv Dip stands for Advanced Diploma
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Children and adults performed on stage and red lucky money envelopes were distributed by William Ho dressed in traditional costume WHO: Catherine Eccles, manager of Guisborough Funeralcare Hub, presents Julee Byrnes, an administrator from the Loftus Funeralcare Branch, with her Level 3 BTEC Advanced Diploma in Funeral Administration and Arranging WHAT: The Salvation Army has opened a new charity shop in Billingham.
A total of 39 students were awarded certificates during the Commissioning Service at Scapegoat Hill Baptist Church, with eight receiving advanced diplomas in counselling.
Students at Robert Gordon University (RGU), Aberdeen, will be able to study a newly created BA Accounting degree, including all the papers needed to achieve the CIMA Advanced Diploma, as a result of an innovative alliance.
Before 1996, for example, the advanced diploma midwives and obstetric doctor team in KZN rural hospitals had full authority to evaluate the care given to mothers in all clinics in their district, provide in-service education to correct defects, improve management protocols, and provide full feed-back on all referrals.
Students who successful passed the country's only International Diploma and Advanced Diploma in Hypnotherapy and Psychotherapy will be awarded certificates in a ceremony at the Elite Grand Hotel, Seef.
This year, more than 4,000 students achieved the Welsh Baccalaureate Advanced Diploma.
Typically students holding the ABE Certificate can gain direct entry onto the first year of a Bachelors degree, holders of the ABE Diploma can get credit from part or all of the first year of a Bachelors degree and holders of the ABE Advanced Diploma can fast-track to the final year of a Bachelors degree.


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UNIT 10.16 Preparation of Hydrogel Substrates with Tunable Mechanical Properties
Published Online: 1 JUN 2010
DOI: 10.1002/0471143030.cb1016s47
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Cell Biology
Additional Information
How to Cite
Tse, J. R. and Engler, A. J. 2010. Preparation of Hydrogel Substrates with Tunable Mechanical Properties. Current Protocols in Cell Biology. 47:10.16:10.16.1–10.16.16.
Publication History
- Published Online: 1 JUN 2010
- Published Print: JUN 2010
- Abstract
- Article
- Figures
- Tables
- References
The modulus of elasticity of the extracellular matrix (ECM), often referred to in a biological context as "stiffness," naturally varies within the body, e.g., hard bones and soft tissue. Moreover, it has been found to have a profound effect on the behavior of anchorage-dependent cells. The fabrication of matrix substrates with a defined modulus of elasticity can be a useful technique to study the interactions of cells with their biophysical microenvironment. Matrix substrates composed of polyacrylamide hydrogels have an easily quantifiable elasticity that can be changed by adjusting the relative concentrations of its monomer, acrylamide, and cross-linker, bis-acrylamide. In this unit, we detail a protocol for the fabrication of statically compliant and radial-gradient polyacrylamide hydrogels, as well as the functionalization of these hydrogels with ECM proteins for cell culture. Included as well are suggestions to optimize this protocol to the choice of cell type or stiffness with a table of relative bis-acrylamide and acrylamide concentrations and expected elasticity after polymerization. Curr. Protoc. Cell Biol. 47:10.16.1-10.16.16. © 2010 by John Wiley & Sons, Inc.
- extracellular matrix;
- elasticity;
- hydrogel;
- gradient;
- polyacrylamide


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|Hospital Quality
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10 Steps to Pick the Right Doctor
Whether you need a primary care doctor or a specialist, these 10 steps will ensure you find the right doctor for you. View Slideshow
Dr. Bahrainwala's Experience
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Medical School
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- Graduated in 1988
Languages Spoken by Dr. Bahrainwala & Staff
- English
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[quote:FRENCHY IS YOUR ENEMY 1351603:MV8yMDY2ODgxXzM0Nzc2NjYwXzk0OEIzQUEx] YOU ARE ABSOLUTELY MAD. THE MIDDLE EAST AS A WHOLE HAS BEEN MANIPULATED BY THE ROMAN CATHOLIC CHURCH THAT SOLD OUT TO THE ASIANS. ITS ALL FRENCH ROTHSCHILD'S MANIPULATIONS. ALWAYS WAS. LEAVE THE MIDDLE EAST ALONE. I WILL NO LONGER LET YOU LIE LIKE THAT. YOU NEED AN ENEMY? TRY MORMONISM. THAT IS WHERE THE SICKNESS AND I DO MEAN THE TRUE SADISM IS. IMAGINE HOW SICK YOU WOULD HAVE TO BE TO KEEP THE "DEAD" ALIVE. POST-HUMANISM/TRANSHUMANISM? WHAT IS THAT REALLY? THINK ABOUT THAT ONE LONG AND HARD. OH WELL THAT IS A JOKE SO YOU DIDN'T NOTICE THE ASIAN TAKEOVER. OBVIOUS TAKEOVER WAS OBVIOUS. OH WE GOT LOTS OF MORMONS IN ASIA. OH WAIT THEY ARE NOT IN ASIA ANYMORE. DAMN, THEY IN THE VERY HOUSE I LIVE IN. WELL, IF THAT IS NOT A COUP I DON'T KNOW WHAT IS. SO HAS YOUR FAMILY MEMBERS BEEN ON THE LEVEL IN APPEARANCE WITH YOU OR IS THERE A SLANT. NOW BE HONEST. [/quote]
Original Message
I have a close friend that worked in many secret black programs and was very knowledgable of many inside secrets. He recently told me a secret that's has been hidden for millennia. I was told that Islam was created in order to cause chaos and destruction on this world and other worlds throughout our quadrant of the galaxy. It was created by the reptilian race of beings who created the basis of Islam from bits and pieces of their own ideology of conquering and expanding their empire. I was told that this is how they infiltrate silently and watch from the shadows as worlds destroy themselves without lifting a finger. This has been done numerous times in the past 25,000 yrs on many worlds. He said once a worlds muslim population reaches more then 45 % it's on it's way to destruction. This happens becaus Islam destroys "infidels" (people who oppose islam) and when no infidels are left they begin to kill each other because of the different sects/factions. He also said that the reason their is a moon for their their flag is because that's where the reptilians control structure is and one of their main bases of operation is (aka their base/interdimensional spacecraft) it's also a powerful image that has certain Qualities to it that cause it to sync with the victims mind in a subliminal way.
He said that's why all Islamic countries have so much death and chaos, especially after the Islamic population reaches 70% and beyond. The reason they are able to do this is because of the negative/dark vibrational frequencies taken in by Muslims while praying, congregating/ or when reading the Koran. Their are certain words in the Koran that actually change chemical processes/DNA in the brain to more of a reptilian function. In which it's normal state is cold, calculating, and domination oriented. Certain words, ideas, and chants put out a frequency/vibrational energy that causes these internal changes.
They also benefit in many other ways. This includes feeding off of the hatred and fear caused in the wars that are created. Also the thousands upon thousands of bodies that are picked up as a food source.
Once a population is dwindled don to a few million, the reptilians come in and take over. They then take what resources they need and use the remaining inhabitants as slaves to mine gold. On that's complete they take the slaves with them on their ship to a new world and use them as a food source during travel and during the beginning phase of the new world they will conquer. The whole process begins again...
He said there a whole lot more info and history to it and he would tell me at a later date. I was shocked at the insights he shared with me.
He's not the type of person to tell fibs, just unbelievable.. I'll update if and when I get new info.
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Gediz University Turkey High School Debate Championship which was held for the 3rd time this year brought together young students from 52 high schools.
Gediz University's University Basketball Team qualified for the Turkish Super League by becoming the undefeated team of the Inter University Basketball First League.
The spring festival of Gediz University, GedizFest attracted a high number of audience with its distinctive schedule which brought together the famous figures of Turkish music and entertainm ...
Gediz University's hybrid power plant which generates electricity from wind and solar energy started to function after an opening ceremony held on Seyrek Campus.
Held by the Gediz University Gender Development Studies, the International Conference on Migration and Gender 2016 brought together a wide range of scholars from 31 countries.
Kavuk: Izmir'in yildizi parliyor 'Gediz Üniversitesi Mütevelli Heyeti Baskani Abdullah Kavuk, "Sekiz bin yillik hosgörü ^^^kültüründen beslenen 41 yasam tarzi, dört mevsimli essiz iklimi, tarihi, dogal güzellikleri, kisacasi çogu buraya özgü tüm zenginlikleriyle Izmir'in yildizi egitimde de parliyor" diyor.
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The 4th annual academic meeting in international political economy will take place from Thursday 21 May to Friday 22 May at Gediz University in Izmir, Turkey. Jointly organized by Leeds Beckett University (Leeds, UK) and Florida International University (Miami, USA), this workshop aims to provide an intellectual forum for scholars of political economy, politics and international relations. We welcome participation from faculty, graduate students, independent scholars, and practitioners analyzing the issues confronting aspects of multilateralism from a variety of disciplines.


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Regulation of Securities - Introduction
Look Out!
The term "security" is vitally important to the USA; therefore, you will find the exact definition from the USA below. You are strongly encouraged to memorize this definition for the exam.
Definition: Stock
As defined by the Uniform Securities Act, asecurity is any of the following:
- a note;
- stock;
- treasury stock;
- security future;
- bond;
- debenture;
- evidence of indebtedness;
- certificate of interest or
- participation in a profit-sharing agreement;
- collateral trust certificate;
- pre-organization certificate or subscription;
- transferable share;
- investment contract;
- voting trust certificate;
- certificate of deposit for a security;
- fractional undivided interest in oil, gas, or other mineral rights;
- put, call, straddle, option, or privilege on a security,
- certificate of deposit, or
- group or index of securities, including an interest therein or based on the value thereof; put, call, straddle, option, or
- privilege entered into on a national securities exchange relating to foreign currency; or, in general,
- an interest or instrument commonly known as a "security"; or
- a certificate of interest or participation in,
- temporary or interim certificate for, receipt for, guarantee of, or
- warrant or right to subscribe to or purchase any of the foregoing.
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Bring a buddy.4.55 / 5.00 10,105 Views
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Vampire Pixel in the Neon City
A sinister deal is underway in the underbelly of Neon City4.34 / 5.00 11,529 Views
Batmans going to kick your ass
Walking around like LADADA!! soo great
i think it's funny. short but still funny
to be honest they weren't bad but the fact that there was only 2 outtakes.......a total waste of my time...
whos was the voice of the guy in the second outtake i forget
That was really nothing new and/or interesting
The first outtake uses a joke as old as cartoon humor goes, and the second is just using a famous movie quote but then with the stick figures. A really big 'meh' for me.


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"I think that nonreligious people respect other people's right to pray," she said. "I know there's plenty of religious people praying that Common Core will stay."
She said she was motivated to write the post out of a feeling of being completely outnumbered by the political power structure of the state and the national marketing power of Common Core supporters. The voices of concerned parents, she said, are being "drowned out" by policymakers and Core lobbyists.
"We're just moms and dads, we don't get a penny for fighting Common Core even through we're doing it daily," she said. "We're doing it because we feel that it is really wrong."
When asked about the PDK/Gallup poll, Swasey said the upside of people being unfamiliar with the Common Core is the potential of more parents becoming informed and joining the opposition.
"It is growing and growing and I predict that it's going to get bigger and louder before it gets quiet," she said.
While a call for prayers can imply a last-ditch effort after other attempts at success have failed, Burbank said that in the case of Utah's Common Core, an appeal to religion shouldn't be seen as evidence that the conversation is over.
"Given the nature of the debate, I suspect that this is much more about rallying the troops to this particular view than it is saying, 'We're getting close to the end and don't know what else to do,'" he said.
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