text:
|Accession Numbers: EG12083 (EcoCyc), b2287, ECK2281
Component of:
NADH:quinone oxidoreductase I, peripheral arm (summary available)
NADH:quinone oxidoreductase I (extended summary available)
NuoB is part of the connecting fragment of NADH dehydrogenase I [Leif95].
This subunit contains the N2 4Fe-4S cluster [Weidner93, Friedrich98a, Flemming03], which may play a role in proton translocation activity of NDH I [Hellwig00]. The Glu67 residue is most likely protonated by oxidation of the N2 cluster, and is involved in proton translocation [Flemming06]. A mechanism by which the redox reaction at the N2 cluster induces conformational changes leading to proton translocation has been proposed [Friedrich10].
Point mutations in NuoB have been analyzed; the Tyr114 and Tyr139 residues appear to be protonated upon reduction of the 4Fe-4S cluster, and a double mutant retains only 20% activity [Flemming03a]. Mutagenesis of the conserved acidic residues E67, D77, and D94 abolishes electron transfer activity of NDH I [Flemming06]. Enzymes containing these mutant subunits have been characterized by FT-IR difference spectroscopy to evaluate conformational changes [Friedrich10].
In the presence of NADH or reducing agents, crosslinking between NuoB and NuoI in the intact Complex I is abolished, indicating a conformational change involving the hydrophilic subunits in the presence of NADH [Berrisford08].
Null mutants of all individual nuo genes have a growth defect under aerobic conditions in rich medium [Erhardt12].
NuoB: "NADH:ubiquinone oxidoreductase" [Calhoun93]
Review: [Flemming05]
Gene Citations: [Bongaerts95, FalkKrzesinski98, Archer95, Choice95]
Locations: inner membrane
|Map Position: [2,403,951 <- 2,404,613] (51.79 centisomes, 186°)
|Length: 663 bp / 220 aa
Molecular Weight of Polypeptide: 25.056 kD (from nucleotide sequence)
pI: 5.83
Unification Links: ASAP:ABE-0007551, CGSC:32676, EchoBASE:EB2008, EcoGene:EG12083, EcoliWiki:b2287, ModBase:P0AFC7, OU-Microarray:b2287, PortEco:nuoB, PR:PRO_000023430, Pride:P0AFC7, Protein Model Portal:P0AFC7, RefSeq:NP_416790, RegulonDB:EG12083, SMR:P0AFC7, String:511145.b2287, Swiss-Model:P0AFC7, UniProt:P0AFC7
Relationship Links: InterPro:IN-FAMILY:IPR006137, InterPro:IN-FAMILY:IPR006138, Panther:IN-FAMILY:PTHR11995, Pfam:IN-FAMILY:PF01058, Prosite:IN-FAMILY:PS01150
In Paralogous Gene Group: 404 (3 members)
Gene-Reaction Schematic
Genetic Regulation Schematic
|Biological Process:
|Molecular Function:
|Cellular Component:
|MultiFun Terms:
|metabolism → energy metabolism, carbon → aerobic respiration
|metabolism → energy metabolism, carbon → anaerobic respiration
|metabolism → energy production/transport → electron donors
|transport → Primary Active Transporters → Oxidoreduction-driven Active Transporters
Essentiality data for nuoB knockouts:
|Growth Medium
|Growth Observations
|LB enriched
|Yes [Gerdes03, Comment 1]
|LB Lennox
|Yes [Baba06, Comment 2]
|M9 medium with 1% glycerol
|Yes [Joyce06, Comment 3]
|MOPS medium with 0.4% glucose
Comment 2]
Yes [Feist07, Comment 4]
Last-Curated 30-Nov-2011 by Keseler I, SRI International
Subunit of: NADH:quinone oxidoreductase I, peripheral arm
Synonyms: soluble NADH dehydrogenase fragment, connecting fragment of NADH dehydrogenase I
Subunit composition of
NADH:quinone oxidoreductase I, peripheral arm = [NuoB][NuoC][NuoE][NuoF][NuoG][NuoI]
NADH:ubiquinone oxidoreductase, chain B = NuoB (extended summary available)
NADH:ubiquinone oxidoreductase, chain CD = NuoC (extended summary available)
NADH:ubiquinone oxidoreductase, chain E = NuoE (summary available)
NADH:ubiquinone oxidoreductase, chain F = NuoF (extended summary available)
NADH:ubiquinone oxidoreductase, chain G = NuoG (extended summary available)
NADH:ubiquinone oxidoreductase, chain I = NuoI (extended summary available)
Component of: NADH:quinone oxidoreductase I (extended summary available)
The soluble NADH dehydrogenase fragment consisting of the NuoE, NuoF and NuoG subunits represents the electron input part of NADH dehydrogenase I [Leif95, Braun98, Bungert99].
The NuoB, NuoCD and NuoI subunits were thought to connect the soluble fragment of NADH dehydrogenase I to the inner membrane components [Leif95].
Subunit of: NADH:quinone oxidoreductase I
Synonyms: NDH-1, NADH dhI, complex I, NADH dehydrogenase I
Subunit composition of
NADH:quinone oxidoreductase I = [NuoA][NuoH][NuoJ][NuoK][NuoL][NuoM][NuoN][(NuoB)(NuoC)(NuoE)(NuoF)(NuoG)(NuoI)]
NADH:ubiquinone oxidoreductase, membrane subunit A = NuoA (extended summary available)
NADH:ubiquinone oxidoreductase, membrane subunit H = NuoH (extended summary available)
NADH:ubiquinone oxidoreductase, membrane subunit J = NuoJ (extended summary available)
NADH:ubiquinone oxidoreductase, membrane subunit K = NuoK (extended summary available)
NADH:ubiquinone oxidoreductase, membrane subunit L = NuoL (extended summary available)
NADH:ubiquinone oxidoreductase, membrane subunit M = NuoM (extended summary available)
NADH:ubiquinone oxidoreductase, membrane subunit N = NuoN (extended summary available)
NADH:quinone oxidoreductase I, peripheral arm = (NuoB)(NuoC)(NuoE)(NuoF)(NuoG)(NuoI) (summary available)
NADH:ubiquinone oxidoreductase, chain B = NuoB (extended summary available)
NADH:ubiquinone oxidoreductase, chain CD = NuoC (extended summary available)
NADH:ubiquinone oxidoreductase, chain E = NuoE (summary available)
NADH:ubiquinone oxidoreductase, chain F = NuoF (extended summary available)
NADH:ubiquinone oxidoreductase, chain G = NuoG (extended summary available)
NADH:ubiquinone oxidoreductase, chain I = NuoI (extended summary available)
NADH:ubiquinone oxidoreductase I (NDH-1) is an NADH dehydrogenase that catalyzes the transfer of electrons from NADH to the quinone pool in the cytoplasmic membrane and is able to generate a proton electrochemical gradient. It is part of both the aerobic and anaerobic respiratory chain of the cell. The study of this enzyme is of great interest, because it is considered to be a structurally minimal form of a proton-pumping NADH:ubiquinone oxidoreductase and serves as a model for the more complex mitochondrial enzyme.
NDH-1 is one of two distinct NADH dehydrogenases in E. coli. In contrast to NDH-2 (encoded by ndh), NDH-1-catalyzed electron flow from NADH to ubiquinone generates an electrochemical gradient. Depending on the strain, NDH-2 utilizes NADH exclusively, while NDH-1 can utilize both NADH and d-NADH, which enables specific assays of the enzyme [Matsushita87, Hayashi89, Calhoun93].
Crystal structures of the membrane domain of NDH-1 have been solved at 3.9 Å resolution [Efremov10] and later at 3 Å resolution [Efremov11]. The striking placement within the NDH-1 complex of NuoL's long amphipathic α-helix suggested that proton translocation may be facilitated by a piston-like movement of this helix [Efremov10, Ohnishi10]. However, analysis of an extensive set of NuoL mutants and analysis of movement of the amphipathic helix of NuoL indicate that it does not function as a mechanical piston and may instead function like a clamp for the NuoLMN subunits during catalytic turnover [Belevich11, Steimle15, Zhu15]. The exact number of protons translocated across the membrane remains unknown; the H+/e- stoichiometry is at least 1.5 [Bogachev96]. Recent experiments argue for at least two coupling sites for proton translocation, with NuoL being essential for the translocation of 2H+/2e- [Steimle11]. A crystal structure of the membrane component at higher resolution has allowed identification of possible proton translocation pathways and argues for a purely conformation-driven pathway of proton translocation [Efremov11]. Zn2+ inhibits Ndh-1, possibly by blocking the entry or exit of a proton translocation pathway [Schulte14].
Based on a stoichiometry of 4 H+ translocated per NADH oxidized (2e-), a mixed model for proton translocation using both direct (redox-driven) and indirect (conformation-driven) mechanisms for proton pumping has been presented [Treberg11]. However, a lower ratio of 3H+/2e- has recently been proposed [Wikstrom12]. The Ndh-1 catalytic cycle has been followed in real time, revealing an essentially biphasic reaction [Belevich14].
The purified enzyme can be separated into three components: a soluble fragment composed of the NuoE, F and G subunits which catalyzes the oxidation of NADH, representing the electron input part of the enzyme [Braun98]; an amphipathic connecting fragment composed of the NuoB, CD and I subunits; and a hydrophobic membrane fragment composed of the NuoA, H, J, K, L, M and N subunits [Leif95]. The soluble subunits contain all iron-sulfur clusters and the FMN cofactor; the redox properties of those cofactors have been studied [Euro08], and their intrinsic redox potential was modeled [Medvedev10]. Electron transfer from NADH via FMN to the iron-sulfur centers has been measured in real time by [Verkhovskaya08] and later by [deVries15], showing different results potentially due to methodological differences. Results from crosslinking analysis suggest that the ubiquinone-binding site of the enzyme is located on the membrane subunit NuoM [Gong03], but it has also been modeled to the interface between NuoB and NuoCD based on its location in the T. thermophilus enzyme [Baranova07]. Site-directed spin labeling is being used for localization of the ubiquinone binding site [Pohl10]. There may be two ubiquinone binding sites [Verkhovsky12], and NDH-1 purified using a new procedure contained two molecules of ubiquinone per complex [Narayanan13]. A tightly bound ubiquinone found by [Verkhovskaya14] has a very low midpoint potential of < -300 mV, while two quinone radicals found by [Hielscher13] had midpoint potentials of -37 and -235 mV. EPR signals from three semiquinone intermediates have been detected, and their properties were characterized [Narayanan15].
The NuoJ [Kao05], NuoK [Kervinen04, Kao05a], NuoM [TorresBacete07] and NuoN [Amarneh03] subunits are implicated in the ability to generate an electrochemical gradient. Molecular dynamics simulations of the membrane domain have established a possible coupling mechanism for energy transduction within NDH-1 [Kaila14].
Three-dimensional reconstruction and 2-D crystals of the NDH-1 complex based on cryo-electron microscopy showed an L-shaped form with an integral membrane and a peripheral arm [Guenebaut98, Holt03]. A model of the spatial arrangement of the subunits and the possible functional mechanism of proton pumping has been proposed [Holt03]. Under low ionic strength conditions, the complex appears to adopt a horseshoe-like conformation [Bottcher02]. Cryo-electron microscopy of the membrane domain allowed calculation of a projection structure at 8 Å resolution [Baranova07]; later, a cryo-EM 3D structure of the intact NDH-1 complex was obtained [Morgan08]. Binding of NADH induces a conformational change in both the membrane and peripheral arm of NDH-1 [Mamedova04, Pohl08]. A mechanism by which the redox reaction of the N2 Fe-S cluster induces a conformational change that may lead to proton translocation has been proposed [Friedrich10].
Heterooligomers of NDH-1 and NDH-2 have been identified by electrophoresis and sucrose gradient centrifugation suggestive of a supramolecular organisation in the membrane [Sousa11].
NDH-1 is required for the anaerobic respiration of NADH using fumarate or DMSO as the terminal electron acceptors, thus implying that the enzyme can transfer electrons to menaquinone [Tran97]. The comparative energy efficiency of utilization of the various components of the aerobic respiratory chain has been examined [Calhoun93a, Unden97].
Stolpe and Friedrich [Stolpe04] showed that NDH-1 is primarily an electrogenic proton pump which may have secondary Na+/H+ antiport activity. However, contrary to the generally accepted view, Steuber et al. [Steuber00] suggested that NDH-1 functions primarily as a Na+ pump, a function that can be conveyed by a truncated form of the NuoL subunit alone [Steuber03, Gemperli07].
NDH-1 produces reactive oxygen species, mainly in the form of H2O2, at the NADH dehydrogenase active site, involving the FMN cofactor [Esterhazy08]. NADH-dependent production of hydrogen peroxide is increased in a NuoF E95Q mutant [Knuuti13]. The rate of O2 reduction is dependent on the NAD+/NADH ratio [Esterhazy08].
Purified NDH-1 is activated by detergent and phospholipids [Sinegina05, Stolpe04]. A tightly bound metal, most likely Ca2+, is required for activity [Verkhovskaya11]. Exposure to tellurite damages NDH-1 [DiazVasquez15].
Mutants lacking NDH-1 can not compete with wild type in stationary phase [Zambrano93]. Expression of the nuo operon is regulated by oxygen, nitrate, fumarate, and other factors including C4 dicarboxylates [Bongaerts95, Tran97]. Transcription and activity of aerobic respiratory chain components in the different phases of aerobic growth have been measured [Sousa12].
Reviews: [Friedrich15, Sato14, NakamaruOgiso14, Sazanov13, Kanjee13, Ohnishi10, Schneider08, Sazanov07, Friedrich04, Yagi03, Friedrich01a, Friedrich00, Ohnishi98, Friedrich98a, Unden97, Neijssel94, ECOSAL]
Citations: [Wong14, Kriegel14]
Locations: inner membrane
Molecular Weight: 550.0 kD (experimental) [Spehr99]
Relationship Links: PDB:Structure:3M9C
|Biological Process:
|Molecular Function:
|Cellular Component:
Last-Curated 21-Dec-2015 by Keseler I, SRI International
Enzymatic reaction of: NADH:ubiquinone oxidoreductase (H+-transporting) (NADH:quinone oxidoreductase I)
Synonyms: ubiquinone reductase, NADH dehydrogenase, respiratory-chain NADH dehydrogenase
EC Number: 22.214.171.124
In Pathways: NADH to cytochrome bd oxidase electron transfer I, NADH to cytochrome bo oxidase electron transfer I
The reaction is reversible in vitro [Poole74], but physiologically unidirectional. The physiological electron acceptor is expected to be ubiquinone-8.
The Km for ubiquinone-2 is 2 µM [Braun98], and for decylubiquinone, 10 µM [Spehr99].
The Ki for piericidin A is 45 nM [Spehr99].
Cofactors or Prosthetic Groups: a [2Fe-2S] iron-sulfur cluster [Braun98, Spehr99, Uhlmann05], a [4Fe-4S] iron-sulfur cluster [Braun98, Spehr99], Ca2+ [Verkhovskaya11], FMN [Hayashi89, Spehr99]Activators (Unknown Mechanism): K+ [Euro09] Inhibitors (Competitive): NAD+ [Euro09a] Inhibitors (Mixed): Zn2+ [Schulte14] Inhibitors (Unknown Mechanism): La3+ [Euro09], piericidin A [David02, Matsushita87, Spehr99], myxothiazol [Matsushita87], o-phenanthroline [Finel94], 3-undecyl-2-hydroxyl-1,4-naphthoquinone [Matsushita87], capsaicin [Satoh96, Yagi90]Kinetic Parameters:
|Km (μM)
Enzymatic reaction of: NADH:menaquinone oxidoreductase (H+-transporting) (NADH:quinone oxidoreductase I)
EC Number: 1.6.5.-
In Pathways: NADH to trimethylamine N-oxide electron transfer, NADH to dimethyl sulfoxide electron transfer, nitrate reduction VIII (dissimilatory), NADH to fumarate electron transfer
|Feature Class
|Pfam PF01058
|64 -> 170
10/20/97 Gene b2287 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12083; confirmed by SwissProt match.
Amarneh03: Amarneh B, Vik SB (2003). "Mutagenesis of subunit N of the Escherichia coli complex I. Identification of the initiation codon and the sensitivity of mutants to decylubiquinone." Biochemistry 42(17);4800-8. PMID: 12718520
Archer95: Archer CD, Elliott T (1995). "Transcriptional control of the nuo operon which encodes the energy-conserving NADH dehydrogenase of Salmonella typhimurium." J Bacteriol 1995;177(9);2335-42. PMID: 7730262
Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554
Baranova07: Baranova EA, Holt PJ, Sazanov LA (2007). "Projection structure of the membrane domain of Escherichia coli respiratory complex I at 8 A resolution." J Mol Biol 366(1);140-54. PMID: 17157874
Belevich11: Belevich G, Knuuti J, Verkhovsky MI, Wikstrom M, Verkhovskaya M (2011). "Probing the mechanistic role of the long α-helix in subunit L of respiratory Complex I from Escherichia coli by site-directed mutagenesis." Mol Microbiol 82(5);1086-95. PMID: 22060017
Belevich14: Belevich N, Belevich G, Verkhovskaya M (2014). "Real-time optical studies of respiratory Complex I turnover." Biochim Biophys Acta 1837(12);1973-1980. PMID: 25283488
Berrisford08: Berrisford JM, Thompson CJ, Sazanov LA (2008). "Chemical and NADH-induced, ROS-dependent, cross-linking between subunits of complex I from Escherichia coli and Thermus thermophilus." Biochemistry 47(39);10262-70. PMID: 18771280
Bogachev96: Bogachev AV, Murtazina RA, Skulachev VP (1996). "H+/e- stoichiometry for NADH dehydrogenase I and dimethyl sulfoxide reductase in anaerobically grown Escherichia coli cells." J Bacteriol 178(21);6233-7. PMID: 8892824
Bongaerts95: Bongaerts J, Zoske S, Weidner U, Unden G (1995). "Transcriptional regulation of the proton translocating NADH dehydrogenase genes (nuoA-N) of Escherichia coli by electron acceptors, electron donors and gene regulators." Mol Microbiol 16(3);521-34. PMID: 7565112
Bottcher02: Bottcher B, Scheide D, Hesterberg M, Nagel-Steger L, Friedrich T (2002). "A novel, enzymatically active conformation of the Escherichia coli NADH:ubiquinone oxidoreductase (complex I)." J Biol Chem 277(20);17970-7. PMID: 11880370
Braun98: Braun M, Bungert S, Friedrich T (1998). "Characterization of the overproduced NADH dehydrogenase fragment of the NADH:ubiquinone oxidoreductase (complex I) from Escherichia coli." Biochemistry 37(7);1861-7. PMID: 9485311
Bungert99: Bungert S, Krafft B, Schlesinger R, Friedrich T (1999). "One-step purification of the NADH dehydrogenase fragment of the Escherichia coli complex I by means of Strep-tag affinity chromatography." FEBS Lett 1999;460(2);207-11. PMID: 10544236
Calhoun93: Calhoun MW, Gennis RB (1993). "Demonstration of separate genetic loci encoding distinct membrane-bound respiratory NADH dehydrogenases in Escherichia coli." J Bacteriol 1993;175(10);3013-9. PMID: 8387992
Calhoun93a: Calhoun MW, Oden KL, Gennis RB, de Mattos MJ, Neijssel OM (1993). "Energetic efficiency of Escherichia coli: effects of mutations in components of the aerobic respiratory chain." J Bacteriol 175(10);3020-5. PMID: 8491720
Choice95: Choice E, Masin D, Bally MB, Meloche M, Madden TD (1995). "Liposomal cyclosporine. Comparison of drug and lipid carrier pharmacokinetics and biodistribution." Transplantation 1995;60(9);1006-11. PMID: 7491673
David02: David P, Baumann M, Wikstrom M, Finel M (2002). "Interaction of purified NDH-1 from Escherichia coli with ubiquinone analogues." Biochim Biophys Acta 1553(3);268-78. PMID: 11997136
deVries15: de Vries S, Dorner K, Strampraad MJ, Friedrich T (2015). "Electron tunneling rates in respiratory complex I are tuned for efficient energy conversion." Angew Chem Int Ed Engl 54(9);2844-8. PMID: 25600069
DiazVasquez15: Diaz-Vasquez WA, Abarca-Lagunas MJ, Cornejo FA, Pinto CA, Arenas FA, Vasquez CC (2015). "Tellurite-mediated damage to the Escherichia coli NDH-dehydrogenases and terminal oxidases in aerobic conditions." Arch Biochem Biophys 566;67-75. PMID: 25447814
ECOSAL: "Escherichia coli and Salmonella: Cellular and Molecular Biology." Online edition.
Efremov10: Efremov RG, Baradaran R, Sazanov LA (2010). "The architecture of respiratory complex I." Nature 465(7297);441-5. PMID: 20505720
Efremov11: Efremov RG, Sazanov LA (2011). "Structure of the membrane domain of respiratory complex I." Nature 476(7361);414-20. PMID: 21822288
Erhardt12: Erhardt H, Steimle S, Muders V, Pohl T, Walter J, Friedrich T (2012). "Disruption of individual nuo-genes leads to the formation of partially assembled NADH:ubiquinone oxidoreductase (complex I) in Escherichia coli." Biochim Biophys Acta 1817(6);863-71. PMID: 22063474
Esterhazy08: Esterhazy D, King MS, Yakovlev G, Hirst J (2008). "Production of reactive oxygen species by complex I (NADH:ubiquinone oxidoreductase) from Escherichia coli and comparison to the enzyme from mitochondria." Biochemistry 47(12);3964-71. PMID: 18307315
Euro08: Euro L, Bloch DA, Wikstrom M, Verkhovsky MI, Verkhovskaya M (2008). "Electrostatic interactions between FeS clusters in NADH:ubiquinone oxidoreductase (Complex I) from Escherichia coli." Biochemistry 47(10);3185-93. PMID: 18269245
Euro08a: Euro L, Belevich G, Verkhovsky MI, Wikstrom M, Verkhovskaya M (2008). "Conserved lysine residues of the membrane subunit NuoM are involved in energy conversion by the proton-pumping NADH:ubiquinone oxidoreductase (Complex I)." Biochim Biophys Acta 1777(9):1166-72. PMID: 18590697
Euro09: Euro L, Belevich G, Wikstrom M, Verkhovskaya M (2009). "High affinity cation-binding sites in Complex I from Escherichia coli." Biochim Biophys Acta 1787(8);1024-8. PMID: 19261245
Euro09a: Euro L, Belevich G, Bloch DA, Verkhovsky MI, Wikstrom M, Verkhovskaya M (2009). "The role of the invariant glutamate 95 in the catalytic site of Complex I from Escherichia coli." Biochim Biophys Acta 1787(1);68-73. PMID: 19061856
FalkKrzesinski98: Falk-Krzesinski HJ, Wolfe AJ (1998). "Genetic analysis of the nuo locus, which encodes the proton-translocating NADH dehydrogenase in Escherichia coli." J Bacteriol 180(5);1174-84. PMID: 9495756
Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909
Finel94: Finel M, Majander A (1994). "Studies on the proton-translocating NADH:ubiquinone oxidoreductases of mitochondria and Escherichia coli using the inhibitor 1,10-phenanthroline." FEBS Lett 339(1-2);142-6. PMID: 8313963
Finn14: Finn RD, Bateman A, Clements J, Coggill P, Eberhardt RY, Eddy SR, Heger A, Hetherington K, Holm L, Mistry J, Sonnhammer EL, Tate J, Punta M (2014). "Pfam: the protein families database." Nucleic Acids Res 42(Database issue);D222-30. PMID: 24288371
Flemming03: Flemming D, Schlitt A, Spehr V, Bischof T, Friedrich T (2003). "Iron-sulfur cluster N2 of the Escherichia coli NADH:ubiquinone oxidoreductase (complex I) is located on subunit NuoB." J Biol Chem 278(48);47602-9. PMID: 12975362
Flemming03a: Flemming D, Hellwig P, Friedrich T (2003). "Involvement of tyrosines 114 and 139 of subunit NuoB in the proton pathway around cluster N2 in Escherichia coli NADH:ubiquinone oxidoreductase." J Biol Chem 278(5);3055-62. PMID: 12446673
Flemming05: Flemming D, Stolpe S, Schneider D, Hellwig P, Friedrich T (2005). "A possible role for iron-sulfur cluster N2 in proton translocation by the NADH: ubiquinone oxidoreductase (complex I)." J Mol Microbiol Biotechnol 10(2-4);208-22. PMID: 16645316
Flemming06: Flemming D, Hellwig P, Lepper S, Kloer DP, Friedrich T (2006). "Catalytic importance of acidic amino acids on subunit NuoB of the Escherichia coli NADH:ubiquinone oxidoreductase (complex I)." J Biol Chem 281(34);24781-9. PMID: 16807239
Friedrich00: Friedrich T, Scheide D (2000). "The respiratory complex I of bacteria, archaea and eukarya and its module common with membrane-bound multisubunit hydrogenases." FEBS Lett 479(1-2);1-5. PMID: 10940377
Friedrich01a: Friedrich T (2001). "Complex I: a chimaera of a redox and conformation-driven proton pump?." J Bioenerg Biomembr 33(3);169-77. PMID: 11695826
Friedrich04: Friedrich T, Bottcher B (2004). "The gross structure of the respiratory complex I: a Lego System." Biochim Biophys Acta 1608(1);1-9. PMID: 14741580
Friedrich10: Friedrich T, Hellwig P (2010). "Redox-induced conformational changes within the Escherichia coli NADH ubiquinone oxidoreductase (complex I): An analysis by mutagenesis and FT-IR spectroscopy." Biochim Biophys Acta 1797:659-63. PMID: 20214873
Friedrich15: Friedrich T, Dekovic DK, Burschel S (2015). "Assembly of the Escherichia coli NADH: Ubiquinone oxidoreductase (respiratory complex I)." Biochim Biophys Acta. PMID: 26682761
Friedrich98a: Friedrich T (1998). "The NADH:ubiquinone oxidoreductase (complex I) from Escherichia coli." Biochim Biophys Acta 1998;1364(2);134-46. PMID: 9593861
Gemperli07: Gemperli AC, Schaffitzel C, Jakob C, Steuber J (2007). "Transport of Na(+) and K (+) by an antiporter-related subunit from the Escherichia coli NADH dehydrogenase I produced in Saccharomyces cerevisiae." Arch Microbiol 188(5);509-21. PMID: 17583799
Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938
GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."
GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."
Gong03: Gong X, Xie T, Yu L, Hesterberg M, Scheide D, Friedrich T, Yu CA (2003). "The ubiquinone-binding site in NADH:ubiquinone oxidoreductase from Escherichia coli." J Biol Chem 278(28);25731-7. PMID: 12730198
Guenebaut98: Guenebaut V, Schlitt A, Weiss H, Leonard K, Friedrich T (1998). "Consistent structure between bacterial and mitochondrial NADH:ubiquinone oxidoreductase (complex I)." J Mol Biol 276(1);105-12. PMID: 9514725
Hayashi89: Hayashi M, Miyoshi T, Takashina S, Unemoto T (1989). "Purification of NADH-ferricyanide dehydrogenase and NADH-quinone reductase from Escherichia coli membranes and their roles in the respiratory chain." Biochim Biophys Acta 977(1);62-9. PMID: 2679883
Hellwig00: Hellwig P, Scheide D, Bungert S, Mantele W, Friedrich T (2000). "FT-IR spectroscopic characterization of NADH:ubiquinone oxidoreductase (complex I) from Escherichia coli: oxidation of FeS cluster N2 is coupled with the protonation of an aspartate or glutamate side chain." Biochemistry 39(35);10884-91. PMID: 10978175
Hielscher13: Hielscher R, Yegres M, Voicescu M, Gnandt E, Friedrich T, Hellwig P (2013). "Characterization of two quinone radicals in the NADH:ubiquinone oxidoreductase from Escherichia coli by a combined fluorescence spectroscopic and electrochemical approach." Biochemistry 52(50);8993-9000. PMID: 24279322
Holt03: Holt PJ, Morgan DJ, Sazanov LA (2003). "The location of NuoL and NuoM subunits in the membrane domain of the Escherichia coli complex I: implications for the mechanism of proton pumping." J Biol Chem 278(44);43114-20. PMID: 12923180
Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394
Kaila14: Kaila VR, Wikstrom M, Hummer G (2014). "Electrostatics, hydration, and proton transfer dynamics in the membrane domain of respiratory complex I." Proc Natl Acad Sci U S A 111(19);6988-93. PMID: 24778264
Kanjee13: Kanjee U, Houry WA (2013). "Mechanisms of acid resistance in Escherichia coli." Annu Rev Microbiol 67;65-81. PMID: 23701194
Kao05: Kao MC, Di Bernardo S, Nakamaru-Ogiso E, Miyoshi H, Matsuno-Yagi A, Yagi T (2005). "Characterization of the membrane domain subunit NuoJ (ND6) of the NADH-quinone oxidoreductase from Escherichia coli by chromosomal DNA manipulation." Biochemistry 44(9);3562-71. PMID: 15736965
Kao05a: Kao MC, Nakamaru-Ogiso E, Matsuno-Yagi A, Yagi T (2005). "Characterization of the membrane domain subunit NuoK (ND4L) of the NADH-quinone oxidoreductase from Escherichia coli." Biochemistry 44(27);9545-54. PMID: 15996109
Kervinen04: Kervinen M, Patsi J, Finel M, Hassinen IE (2004). "A pair of membrane-embedded acidic residues in the NuoK subunit of Escherichia coli NDH-1, a counterpart of the ND4L subunit of the mitochondrial complex I, are required for high ubiquinone reductase activity." Biochemistry 43(3);773-81. PMID: 14730982
Knuuti13: Knuuti J, Belevich G, Sharma V, Bloch DA, Verkhovskaya M (2013). "A single amino acid residue controls ROS production in the respiratory Complex I from Escherichia coli." Mol Microbiol 90(6);1190-200. PMID: 24325249
Kriegel14: Kriegel S, Uchida T, Osawa M, Friedrich T, Hellwig P (2014). "Biomimetic Environment to Study E. coli Complex I through Surface-Enhanced IR Absorption Spectroscopy." Biochemistry 53(40);6340-7. PMID: 25225967
Lasserre06: Lasserre JP, Beyne E, Pyndiah S, Lapaillerie D, Claverol S, Bonneu M (2006). "A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis." Electrophoresis 27(16);3306-21. PMID: 16858726
Leif95: Leif H, Sled VD, Ohnishi T, Weiss H, Friedrich T (1995). "Isolation and characterization of the proton-translocating NADH: ubiquinone oxidoreductase from Escherichia coli." Eur J Biochem 230(2);538-48. PMID: 7607227
Mamedova04: Mamedova AA, Holt PJ, Carroll J, Sazanov LA (2004). "Substrate-induced conformational change in bacterial complex I." J Biol Chem 279(22);23830-6. PMID: 15037611
Matsushita87: Matsushita K, Ohnishi T, Kaback HR (1987). "NADH-ubiquinone oxidoreductases of the Escherichia coli aerobic respiratory chain." Biochemistry 1987;26(24);7732-7. PMID: 3122832
Medvedev10: Medvedev ES, Couch VA, Stuchebrukhov AA (2010). "Determination of the intrinsic redox potentials of FeS centers of respiratory complex I from experimental titration curves." Biochim Biophys Acta 1797(9):1665-71. PMID: 20513348
Morgan08: Morgan DJ, Sazanov LA (2008). "Three-dimensional structure of respiratory complex I from Escherichia coli in ice in the presence of nucleotides." Biochim Biophys Acta 1777(7-8);711-8. PMID: 18433710
NakamaruOgiso14: Nakamaru-Ogiso E, Narayanan M, Sakyiama JA (2014). "Roles of semiquinone species in proton pumping mechanism by complex I." J Bioenerg Biomembr 46(4);269-77. PMID: 25077450
Narayanan13: Narayanan M, Gabrieli DJ, Leung SA, Elguindy MM, Glaser CA, Saju N, Sinha SC, Nakamaru-Ogiso E (2013). "Semiquinone and cluster N6 signals in His-tagged proton-translocating NADH:ubiquinone oxidoreductase (complex I) from Escherichia coli." J Biol Chem 288(20);14310-9. PMID: 23543743
Narayanan15: Narayanan M, Leung SA, Inaba Y, Elguindy MM, Nakamaru-Ogiso E (2015). "Semiquinone intermediates are involved in the energy coupling mechanism of E. coli complex I." Biochim Biophys Acta 1847(8);681-9. PMID: 25868873
Neijssel94: Neijssel OM, Teixeira de Mattos MJ (1994). "The energetics of bacterial growth: a reassessment." Mol Microbiol 13(2);172-82. PMID: 7984099
Ohnishi10: Ohnishi T (2010). "Structural biology: Piston drives a proton pump." Nature 465(7297);428-9. PMID: 20505714
Ohnishi98: Ohnishi T (1998). "Iron-sulfur clusters/semiquinones in complex I." Biochim Biophys Acta 1364(2);186-206. PMID: 9593887
Park06: Park YJ, Yoo CB, Choi SY, Lee HB (2006). "Purifications and characterizations of a ferredoxin and its related 2-oxoacid:ferredoxin oxidoreductase from the hyperthermophilic archaeon, Sulfolobus solfataricus P1." J Biochem Mol Biol 39(1);46-54. PMID: 16466637
Pohl07: Pohl T, Uhlmann M, Kaufenstein M, Friedrich T (2007). "Lambda Red-mediated mutagenesis and efficient large scale affinity purification of the Escherichia coli NADH:ubiquinone oxidoreductase (complex I)." Biochemistry 46(37);10694-702. PMID: 17722886
Pohl08: Pohl T, Schneider D, Hielscher R, Stolpe S, Dorner K, Kohlstadt M, Bottcher B, Hellwig P, Friedrich T (2008). "Nucleotide-induced conformational changes in the Escherichia coli NADH:ubiquinone oxidoreductase (complex I)." Biochem Soc Trans 36(Pt 5);971-5. PMID: 18793172
Pohl10: Pohl T, Spatzal T, Aksoyoglu M, Schleicher E, Rostas AM, Lay H, Glessner U, Boudon C, Hellwig P, Weber S, Friedrich T (2010). "Spin labeling of the Escherichia coli NADH ubiquinone oxidoreductase (complex I)." Biochim Biophys Acta. PMID: 20959113
Poole74: Poole RK, Haddock BA (1974). "Energy-linked reduction of nicotinamide--adenine dinucleotide in membranes derived from normal and various respiratory-deficient mutant strains of Escherichia coli K12." Biochem J 144(1);77-85. PMID: 4156832
Sato14: Sato M, Torres-Bacete J, Sinha PK, Matsuno-Yagi A, Yagi T (2014). "Essential regions in the membrane domain of bacterial complex I (NDH-1): the machinery for proton translocation." J Bioenerg Biomembr 46(4);279-87. PMID: 24973951
Satoh96: Satoh T, Miyoshi H, Sakamoto K, Iwamura H (1996). "Comparison of the inhibitory action of synthetic capsaicin analogues with various NADH-ubiquinone oxidoreductases." Biochim Biophys Acta 1273(1);21-30. PMID: 8573592
Sazanov07: Sazanov LA (2007). "Respiratory complex I: mechanistic and structural insights provided by the crystal structure of the hydrophilic domain." Biochemistry 46(9);2275-88. PMID: 17274631
Sazanov13: Sazanov LA, Baradaran R, Efremov RG, Berrisford JM, Minhas G (2013). "A long road towards the structure of respiratory complex I, a giant molecular proton pump." Biochem Soc Trans 41(5);1265-71. PMID: 24059518
Schneider08: Schneider D, Pohl T, Walter J, Dorner K, Kohlstadt M, Berger A, Spehr V, Friedrich T (2008). "Assembly of the Escherichia coli NADH:ubiquinone oxidoreductase (complex I)." Biochim Biophys Acta 1777(7-8);735-9. PMID: 18394423
Schulte14: Schulte M, Mattay D, Kriegel S, Hellwig P, Friedrich T (2014). "Inhibition of Escherichia coli Respiratory Complex I by Zn(2+)." Biochemistry 53(40);6332-9. PMID: 25238255
Sinegina05: Sinegina L, Wikstrom M, Verkhovsky MI, Verkhovskaya ML (2005). "Activation of isolated NADH:ubiquinone reductase I (complex I) from Escherichia coli by detergent and phospholipids. Recovery of ubiquinone reductase activity and changes in EPR signals of iron-sulfur clusters." Biochemistry 44(23);8500-6. PMID: 15938640
Sousa11: Sousa PM, Silva ST, Hood BL, Charro N, Carita JN, Vaz F, Penque D, Conrads TP, Melo AM (2011). "Supramolecular organizations in the aerobic respiratory chain of Escherichia coli." Biochimie 93(3);418-25. PMID: 21040753
Sousa12: Sousa PM, Videira MA, Bohn A, Hood BL, Conrads TP, Goulao LF, Melo AM (2012). "The aerobic respiratory chain of Escherichia coli: from genes to supercomplexes." Microbiology 158(Pt 9);2408-18. PMID: 22700653
Spehr99: Spehr V, Schlitt A, Scheide D, Guenebaut V, Friedrich T (1999). "Overexpression of the Escherichia coli nuo-operon and isolation of the overproduced NADH:ubiquinone oxidoreductase (complex I)." Biochemistry 1999;38(49);16261-7. PMID: 10587449
Steimle11: Steimle S, Bajzath C, Dorner K, Schulte M, Bothe V, Friedrich T (2011). "Role of subunit NuoL for proton translocation by respiratory complex I." Biochemistry 50(16);3386-93. PMID: 21417432
Steimle15: Steimle S, Schnick C, Burger EM, Nuber F, Kramer D, Dawitz H, Brander S, Matlosz B, Schafer J, Maurer K, Glessner U, Friedrich T (2015). "Cysteine scanning reveals minor local rearrangements of the horizontal helix of respiratory complex I." Mol Microbiol 98(1);151-61. PMID: 26115017
Steuber00: Steuber J, Schmid C, Rufibach M, Dimroth P (2000). "Na+ translocation by complex I (NADH:quinone oxidoreductase) of Escherichia coli." Mol Microbiol 2000;35(2);428-34. PMID: 10652103
Steuber03: Steuber J (2003). "The C-terminally truncated NuoL subunit (ND5 homologue) of the Na+-dependent complex I from Escherichia coli transports Na+." J Biol Chem 278(29);26817-22. PMID: 12740360
Stolpe04: Stolpe S, Friedrich T (2004). "The Escherichia coli NADH:ubiquinone oxidoreductase (complex I) is a primary proton pump but may be capable of secondary sodium antiport." J Biol Chem 279(18);18377-83. PMID: 14970214
TorresBacete07: Torres-Bacete J, Nakamaru-Ogiso E, Matsuno-Yagi A, Yagi T (2007). "Characterization of the NuoM (ND4) subunit in Escherichia coli NDH-1: Conserved charged residues essential for energy-coupled activities." J Biol Chem 282(51);36914-22. PMID: 17977822
Tran97: Tran QH, Bongaerts J, Vlad D, Unden G (1997). "Requirement for the proton-pumping NADH dehydrogenase I of Escherichia coli in respiration of NADH to fumarate and its bioenergetic implications." Eur J Biochem 244(1);155-60. PMID: 9063459
Treberg11: Treberg JR, Brand MD (2011). "A model of the proton translocation mechanism of complex I." J Biol Chem 286(20);17579-84. PMID: 21454533
Uhlmann05: Uhlmann M, Friedrich T (2005). "EPR Signals Assigned to Fe/S Cluster N1c of the Escherichia coli NADH:Ubiquinone Oxidoreductase (Complex I) Derive from Cluster N1a." Biochemistry 44(5);1653-8. PMID: 15683249
Unden97: Unden G, Bongaerts J (1997). "Alternative respiratory pathways of Escherichia coli: energetics and transcriptional regulation in response to electron acceptors." Biochim Biophys Acta 1320(3);217-34. PMID: 9230919
UniProt15: UniProt Consortium (2015). "UniProt version 2015-08 released on 2015-07-22." Database.
UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."
UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."
Verkhovskaya08: Verkhovskaya ML, Belevich N, Euro L, Wikstrom M, Verkhovsky MI (2008). "Real-time electron transfer in respiratory complex I." Proc Natl Acad Sci U S A 105(10);3763-7. PMID: 18316732
Verkhovskaya11: Verkhovskaya M, Knuuti J, Wikstrom M (2011). "Role of Ca(2+) in structure and function of Complex I from Escherichia coli." Biochim Biophys Acta 1807(1);36-41. PMID: 20840839
Verkhovskaya14: Verkhovskaya M, Wikstrom M (2014). "Oxidoreduction properties of bound ubiquinone in Complex I from Escherichia coli." Biochim Biophys Acta 1837(2);246-50. PMID: 24216024
Verkhovsky12: Verkhovsky M, Bloch DA, Verkhovskaya M (2012). "Tightly-bound ubiquinone in the Escherichia coli respiratory complex I." Biochim Biophys Acta 1817(9);1550-6. PMID: 22580197
Weidner93: Weidner U, Geier S, Ptock A, Friedrich T, Leif H, Weiss H (1993). "The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I." J Mol Biol 1993;233(1);109-22. PMID: 7690854
Wikstrom12: Wikstrom M, Hummer G (2012). "Stoichiometry of proton translocation by respiratory complex I and its mechanistic implications." Proc Natl Acad Sci U S A 109(12);4431-6. PMID: 22392981
Wong14: Wong KS, Snider JD, Graham C, Greenblatt JF, Emili A, Babu M, Houry WA (2014). "The MoxR ATPase RavA and Its Cofactor ViaA Interact with the NADH:Ubiquinone Oxidoreductase I in Escherichia coli." PLoS One 9(1);e85529. PMID: 24454883
Yagi03: Yagi T, Matsuno-Yagi A (2003). "The proton-translocating NADH-quinone oxidoreductase in the respiratory chain: the secret unlocked." Biochemistry 42(8);2266-74. PMID: 12600193
Yagi90: Yagi T (1990). "Inhibition by capsaicin of NADH-quinone oxidoreductases is correlated with the presence of energy-coupling site 1 in various organisms." Arch Biochem Biophys 281(2);305-11. PMID: 2118334
Zambrano93: Zambrano MM, Kolter R (1993). "Escherichia coli mutants lacking NADH dehydrogenase I have a competitive disadvantage in stationary phase." J Bacteriol 175(17);5642-7. PMID: 8366049
Zhu15: Zhu S, Vik SB (2015). "Constraining the Lateral Helix of Respiratory Complex I by Cross-linking Does Not Impair Enzyme Activity or Proton Translocation." J Biol Chem 290(34);20761-73. PMID: 26134569
Kumar11: Kumar R, Shimizu K (2011). "Transcriptional regulation of main metabolic pathways of cyoA, cydB, fnr, and fur gene knockout Escherichia coli in C-limited and N-limited aerobic continuous cultures." Microb Cell Fact 10;3. PMID: 21272324
Lynch96: Lynch AS, Lin EC (1996). "Transcriptional control mediated by the ArcA two-component response regulator protein of Escherichia coli: characterization of DNA binding at target promoters." J Bacteriol 1996;178(21);6238-49. PMID: 8892825
Marzan13: Marzan LW, Hasan CM, Shimizu K (2013). "Effect of acidic condition on the metabolic regulation of Escherichia coli and its phoB mutant." Arch Microbiol 195(3);161-71. PMID: 23274360
Salmon03: Salmon K, Hung SP, Mekjian K, Baldi P, Hatfield GW, Gunsalus RP (2003). "Global gene expression profiling in Escherichia coli K12. The effects of oxygen availability and FNR." J Biol Chem 278(32);29837-55. PMID: 12754220
Salmon05: Salmon KA, Hung SP, Steffen NR, Krupp R, Baldi P, Hatfield GW, Gunsalus RP (2005). "Global gene expression profiling in Escherichia coli K12: effects of oxygen availability and ArcA." J Biol Chem 280(15);15084-96. PMID: 15699038
ShalelLevanon05: Shalel-Levanon S, San KY, Bennett GN (2005). "Effect of oxygen, and ArcA and FNR regulators on the expression of genes related to the electron transfer chain and the TCA cycle in Escherichia coli." Metab Eng 7(5-6):364-74. PMID: 16140031
Wackwitz99: Wackwitz B, Bongaerts J, Goodman SD, Unden G (1999). "Growth phase-dependent regulation of nuoA-N expression in Escherichia coli K-12 by the Fis protein: upstream binding sites and bioenergetic significance." Mol Gen Genet 262(4-5);876-83. PMID: 10628873
Zhang04: Zhang J, Zeuner Y, Kleefeld A, Unden G, Janshoff A (2004). "Multiple site-specific binding of Fis protein to Escherichia coli nuoA-N promoter DNA and its impact on DNA topology visualised by means of scanning force microscopy." Chembiochem 5(9);1286-9. PMID: 15368583
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Cordillera Blanca > Chopicalqui > Climber's Log
Chopicalqui Climber's Log
[ Sign the Climber's Log ]
|SW Ridge
Date Climbed: Jul 1, 2007
|Great climb to acclimatize, with some good exposure across the bergschrund to the summit pyramid. Not as technically difficult as the guide book suggested, but there are some sections where pitching it out could be helpful (or prudent). Had a few issues with bottlenecks and guided groups/inexperienced climbers - made things a little more dangerous than i would have liked.
|Posted Oct 20, 2009 1:21 pm
|SW Ridge
Date Climbed: Jun 13, 2008
|A long route to a high peak without much (if any) technical climbing. Great views of Huascaran and the rest of the Blanca. Conditions were very good, nice to find after reports of heavy snow and no prior ascents during the season.
|Posted Sep 30, 2009 5:34 pm
|Normal route
|Reached the summit together with my peruvian friend Julver. The night before we brought a mexican couple down to basecamp because of sorroche, slept 2 hours & started the climb. Some sketchy climbing on the summit mushroom (around 80 degree) with bad protection. Rappeled on a snow bollard and descended to the low camp. Long but unforgetable day!
|Posted Sep 1, 2009 6:12 pm
|Brad F
|South West Ridge
Date Climbed: Jun 25, 2009
|Amazing climb! It snowed every other day except for our summit day :)
|Posted Jul 7, 2009 1:55 pm
|SW Ridge
Date Climbed: Jun 18, 2006
|Climbed with Peruvian guide, Willaim.
|Posted Jul 4, 2009 1:24 am
|SW ridge
Date Climbed: Jun 12, 2009
|Great climb, good snow conditions in the early morning. A couple of steep snow pitches and an exposed passage on the summit mushroom but nothing too bad.
Day 1: trailhead (4200m) to Moraine camp (5000m), skipping BC. Pretty awful load carrying on a steep trail, maybe a porter would have been a good idea. 4h30 total.
Day 2: Moraine camp (5000m) to High Camp (5600m). Glacier terrain but quite easy, unroped travel possible. Pretty grueling as I was still tired from the previous day. 4h instead of the 2/3 usually recommended.
Day 3: High Camp (5600m) to Summit (6354m) and back to Moraine Camp. Left at 2am, summited at 9am along with two other parties. Snow was in good condition and we didn't see any ice at all. Tracks were already made from the past week. It took us forever to descend the technical parts at the top due to merging with the Columbian party, and 4 persons abseils are very slow. 7h to summit, 5h back to high camp, 45 minutes to moraine camp.
Day 4: Moraine camp to BC (1h30) to trailhead (15 minutes) and taxi to Huaraz.
|Posted Jun 13, 2009 7:10 pm
|SW Ridge
Date Climbed: Jun 26, 2007
|Spent an extra day at the Moraine Camp to wait out weather. Summited from Moraine Camp and down in a day. long day... Climbed with Peter (see below, I'm one of the Toms..) and a couple of Brits we met at the Moraine camp.
|Posted Nov 27, 2008 1:39 pm
|Route climbed: SW ridge
Date Climbed: Jul 9, 2007
|Summited in a whiteout after a hurried ascent to avoid getting stuck away from Huaraz during the strike. Beautiful mountain and approach, wish we could have seen something from the summit though! With Derek, Oscar, and Carlos.
|Posted Jul 22, 2008 6:25 pm
Date Climbed: May 16, 2007
|Were first up for 2007 season according to guides, definetly no signs of others. Plodded steps through deep powder, luckily were well acclimatized from two months trekking and climbing. Made it to summit shoulder but could not climb final pitch, extreme snow wall, no ice, lots of crumbly snow. Hard one to swallow but went down safely to climb again.
|Posted Dec 12, 2007 1:18 am
|sw ridge
Date Climbed: Jul 5, 2007
|high point so far, super fun except for the guided cluster disaster ahead of us
|Posted Oct 24, 2007 1:22 am
|The SW Ridge
Date Climbed: Aug 3, 2007
|Climbed with T. Haines. After being a little confused about where high camp was supposed to be and placing the highest high camp ever on the normal route at 5950 m. we got to the summit in no time! Last 400 meters of the mountain are very interesting and included a short section of climbing over an overhanging serac! This route is not in the best condition and right now is probably the grade of D. We got to the summit, broke camp, and got all the way to the road in the same day. 12 hours total.
|Posted Aug 8, 2007 6:47 pm
|SW ridge
Date Climbed: Jun 26, 2007
|Great outing with 3 Americans I met in Cebollapampa (John, Tom and Tom)
day 1 - hike into moraine camp (sketchy getting water from glacier)
day 2 - snow on and off all day, wait out bad weather in camp
day 3 - wake up at midnight, 7-8 hours to top from moraine camp, return, pack up and descend all the way to base camp. cold and windy at the top, but not terrible. long day
The weather was sketchy for the past 2 weeks and we seemed to have hit the first good window... surprisingly clear night!
|Posted Jun 29, 2007 2:03 pm
|alsmost there
Date Climbed: Jun 19, 2005
|Fifth Peak (and highest)iin two weeks and I ws pooped. Two many people as Huascaran and Alpamayo were shut down
|Posted Aug 15, 2006 9:16 pm
|Tom Fralich
|SW Ridge
Date Climbed: Jul 11, 2006
|Climbed with Dave Greenhouse in 4 hours from Col Camp at 18,500 ft. The route was in very good condition with well consolidated snow and no major crevasse issues. Traversing through the seracs at 20,000 ft was a bit scary, with lots of debris already on the ground. In total we spent 6 days on the mountain.
Day 1...Trailhead (4200m) to Moraine Camp (5000m)
Day 2...Carry to Col Camp (5600m), return to Moraine Camp
Day 3...Move to Col Camp
Day 4...Summit (6354m), return to Moraine Camp
Day 5...Rest in Moraine Camp, an extra day in case of bad weather, illness
Day 6...Moraine Camp to Trailhead and taxi to Huaraz
Unfortunately, Dave suffered some reasonably bad frostbite to three of his fingers on summit day and had to return to the US after completing this climb.
|Posted Jul 13, 2006 10:27 pm
|S/W Ridge
Date Climbed: Jul 30, 1998
|It was a long day. We started from morain camp at 16,860 in the dark and finished in the dark 15hrs. latter
|Posted Apr 14, 2006 4:39 pm
|Route Climbed: SW Ridge Date Climbed: July 21, 2005
|Very enjoyable and interesting route.
|Posted Aug 8, 2005 12:37 am
|Route Climbed: South West Ridge Date Climbed: July 1, 2005
|Summited with my Peruvian guide, Aldo on a gloriously clear and calm morning just after sunrise with the moon just above the summit. 65-70 degree 100 meter pitch above the bergschrund The panarama of the Cordillera Blanca was fantastic. Five minutes after we summited, Brad Johnson (author of Classic Climbs of the Cordillera Blanca) summited with 2 clients. Spent an enjoyable 20 minutes on top.
|Posted Jul 5, 2005 5:44 pm
|Route Climbed: NW Ridge Date Climbed: August 15-22, 2002
|Our old guidebook said, "Allow 3 days to return to the road." We packed enough food for 4 days, and it took us 7. Fortunately, we greatly overestimated how much fuel we would need, and this was a life saver. The NW ridge involved lots of scary, loose, honeycombed snow. I thought I was going to die in a few places, but the position and exposure are absolutely spectacular!
Trip Report and more photos here:
|Posted Mar 22, 2005 7:09 pm
|Route Climbed: sw-ridge Date Climbed: july 04
|great climb with perfect view...did some variant and climed airy ridge to avoid avalanche-danger in upper section
david bruder&niv scott
|Posted Sep 12, 2004 11:24 pm
|Route Climbed: southeast slopes / south ridge Date Climbed: 12 June 1971
|Tom Marron and I climbed this peak from our base camp near the Yanganuca Lakes in near perfect weather. For the tragic aspects of this climb see the trip report.
|Posted May 17, 2004 5:24 pm

subset: commoncrawl


text:
Oh, you didn't know Notes from HeL had a book club?
Yeah, well, neither did we -- until we started to write this blog entry.
But why not? Oprah's not the boss of book clubs. OK. maybe she is -- but I digress.
In case you missed it, our first pick was Junot Diaz' "The Brief Wondrous Life of Oscar Wao." It's a great read -- and Hartford's One Book this year. If you haven't read it yet, get yourself to the library or a bookstore and grab it -- Diaz is coming to Hartford on Oct. 23.
But here's the next must-read for the Notes from HeL Book Club members, of which there's a grand total of one -- so far.
It's a heart-wrenching story of a teen who commits suicide and the 13 reasons she decides to end her life.
Here's a little from the inside flap: "Clay Jensen returns home from school to find a strange packet with his name on it lying on his porch. Inside, he discovers several cassette tapes recorded by Hannah Baker -- his classmate and crush -- who committed suicide two weeks earlier."
It's a reminder -- to all of us, no matter what our age -- how powerful, and potentially devastating words and actions can be.
If you are a teen, have a teen, or know a teen, read this important book.
So there you have it NFH Book Club members -- this month's pick.
Tell me what you think. And feel free to offer suggestions for next month's pick.


text:
New Destinations in Japan 2012
New York, NY, November 3, 2011:Japan's recent new tourism landmarks will signify Japan's cultural and industrial richness. Cherishing the unique history as well as advancing the efficient technology, the new facilities showcase Japan's both past and future.
Nagasaki opened the 400 years old warehouse that was used by the East India Company in September 2011. Nagasaki was the first base in Japan for the European merchants and travelers, since the self-defensing samurai-run Japanese government only opened a small area in Nagasaki for the Westerners. The warehouse was originally built by the East India Company, and the September opening made the un-Japanese looking architecture into a museum of the European influence in the Hirado district, how the East India Company reached to Nagasaki, and Nagasaki's unique encounter with the European (particularly Dutch) culture in the era when Japan was closed to the rest of the world. (http://hirado-shoukan.jp/ Japanese only)
Michelin started releasing the 2012 guides with two more Japanese cities for the world famous restaurant guides. Nara, the very first capital of Japan, joined the Kyoto/Osaka/Kobe Restaurant Guide from the 2012 edition. Michelin has already given the most number of stars to the Japanese guides, and the 25 restaurants in total are added from Nara. Hokkaido, Japan's northern island with the Olympic quality powder snow and endless agricultural lands, will be showcased in the Michelin Restaurant Guide, which is exclusively released in Japanese.
Japan Airlines will begin nonstop service between Boston Logan and Tokyo's Narita International Airport starting April 22, 2012, and the airline will operate the route using its brand-new Boeing 787 Dreamliner aircraft. This first route to directly link Boston with Asia will launch with four weekly flights (Mondays, Wednesdays, Fridays and Sundays), and increase to daily service from June 1, 2012. The route will also be a code-share by American Airlines, who entered into a joint business agreement with JAL in April 2011.
Japan's capital city is getting a new digital transmission tower in the era of digital broadcasting and mobile devices. Named as Tokyo Sky Tree, the new transmission tower is 634 meter high (2,080 feet), and will be completed as the world's tallest free-standing tower. Equipped with Japanese high-tech structures and employed the traditional architectural method of center column vibration control from the five-story pagoda, the Tokyo Sky Tree is the latest seismic proof tower. The tower easily surpasses height of the current Tokyo Tower (250m, 820ft) and other skyscrapers in Tokyo, creating a new skyline of Tokyo. The tower's grand opening is on May 22, 2012.
A new commercial tower emerges near the iconic enormous crossing in Shibuya. Hikarié is in the final stage for the opening in summer 2012, in the vicinity of Shibuya train station. Housing theatres, galleries, event space and shopping floors, Hikarié will become the ground zero of Tokyo's art, culture, contents, and the information. In tandem with many other theatres, galleries, cafés, clubs and stores, Shibuya has been culturally influential, and Hikarié will absorb and produce pop culture trends.
International Monetary Fund (IMF) and World Bank will meet in Tokyo for the 2012 Annual Meetings' venue. The two largest international finance organizations picked Tokyo as an alternative venue for the 2012 event after Egypt, the original host country, gave up to host the meeting. Starting from October 9, 2012, the Japanese capital city expects more than 20,000 visitors related to the meeting. This is the first time to have IMF and World Bank meeting in Japan since 1964.
For more information contact:
Nori Akashi, Public Relations Manager
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Tags: Les Miserables, J. Mark McVey, Andrew Varela, Betsy Morgan, Chasten Harmon, Julie Benko, Max Quinlan, Richard Vida, Shawna M. Hamic, I Dreamed a Dream, On My Own, Glee, Susan Boyle, Hugh Jackman, Russell Crowe, Anne Hathaway, Samantha Barks, Next to Normal, The Normal Heart, Seth Rudetsky, Lea Michele, Sarah Silverman, Opera, Theatre, Theatre Reviews
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Stripe Duvet Cover + Shams - Lotus Pink
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Dating back to the 13th century, this property offers friendly and attentive service. The stylish bedrooms feature finest Italian cotton linens, flat-screen TVs and WiFi. There are five types of room ...More about BridgeHouse


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MFA in Applied Craft and Design Presents Zahid Sardar
MFA in Applied Craft and Design presents a lecture by design writer and editor Zahid Sardar on Thursday, October 22.
Sardar is the author and designer of New Garden Design and San Francisco Modern. He has written about architecture, interiors, craft and design for the San Francisco Examiner, and the San Francisco Chronicle where, for 20 years, he has held various titles including Architecture & Design Editor for the Chronicle magazine and most recently, San Francisco Chronicle Design Editor.
Sardar has lectured widely about architecture, interior design and garden design and has participated on design juries for various organizations including the American Institute of Architects. His current lectures at Bay Area schools about the history of design focus on the return and value of craft in industrial design. Sardar has also written for House & Garden, House Beautiful, Elle Décor, Metropolis, and Architecture, among others.
MFA in Applied Craft and Design Lecture | Zahid Sardar
6:30 p.m. October 22 | MFA in Applied Craft and Design Studios @ The Bison Building, 421 N.E. 10th Ave.
Pecha Kucha–Inspired Night at Museum of Contemporary Craft
Join the Museum of Contemporary Craft and Pacific Northwest College of Art's MFA in Applied Craft and Design program (a joint MFA offered with Oregon College of Art and Craft) for an energetic, casual Pecha Kucha-inspired Night.
"Pecha Kucha":http://www.pecha-kucha.org/ is a concept that grew out of the Tokyo design community, featuring a series of concise presentations. MFA in Applied Craft and Design students will present ideas and images in a modified format of roughly 3.5 minutes each. This lively program will introduce the audience to ideas, objects, and questions on the minds of the inaugural class of this innovative new program.
Special Event | Pecha Kucha–Inspired Night
5:30 p.m., Wednesday, October 21
Lab at the Museum of Contemporary Craft, 724 N.W. Davis St.
Faculty Biennial Closes Soon
Don't miss the last chance to view Pacific Northwest College of Art's centennial Faculty Biennial, showcasing the work of its faculty—the exhibition closes October 17.
Over the course of the last century, PNCA's faculty have been some of the leaders in visual thinking and making. That reputation remains and is showcased in the 2009 Faculty Biennial Exhibition, featuring the work of the College's current 85 faculty members.
Exhibition | Faculty Biennial
Now through October 17
PNCA Main Campus Building, Swigert Commons, 1241 N.W. Johnson St.
Watch and Listen: Etsy Slideshow and Podcast
Pacific Northwest College of Art welcomed Etsy's Matt Stinchcomb for the PNCA - Cyan/PDX Cultural Residency Series, including a public lecture, Digitally Building the DIY Community, and a range of interactions with the PNCA community.
Portland's DIY culture continues to be recognized internationally as a central hub of the American DIY movement, celebrated as a collective of creative talent with a progressive approach to the entrepreneurial spirit. Presented by the PNCA – Cyan/PDX Cultural Residency Series.
"Listen to a podcast of Digitally Building the DIY Community: A Conversation with Matt Stinchcomb of Etsy":http://homeroom.pnca.edu/download/586639
MFA in Visual Studies Presents Alix Pearlstein
New York-based artist Alix Pearlstein will discuss her wide range of work that includes conceptual sculpture, multi-channel installation, and performance-based video during a lecture at 12:30 p.m. Wednesday, October 14 in the College's Swigert Commons.
Pearlstein uses everyday objects, pop cultural artifacts, and art historical references to create narrative meaning through association. Characterized by deadpan humor and a minimalist aesthetic, Pearlstein's approach is direct and intimate. Her videotapes engage in wry, self-conscious renderings of icons of style, gender, media, and art historical Modernism.
Her works have been included in exhibitions at the Biennale de Lyon, France; Institute of Contemporary Art, London; Kunstverein, Cologne; S.M.A.K., Ghent, and The Museum of Modern Art, New York. One-person exhibitions of her work have been seen at The Kitchen, NYC; The MIT List Visual Arts Center, Cambridge MA; Museum of Contemporary Art, Chicago; Postmasters Gallery and Artemis Greenberg Van Doren Gallery, New York; and The Grossman Gallery, School of The Museum of Fine Arts, Boston.
MFA In Visual Studies Lecture| Alix Pearlstein
12:30 p.m. Wednesday, October 14 | PNCA Main Campus Building, Swigert Commons, 1241 N.W. Johnson St.
Photo courtesy of the artist.
MFA in Visual Studies Presents Artist Kate Gilmore
PNCA's MFA in Visual Studies program presents Kate Gilmore in a lecture, 12:30 p.m. Thursday, October 15 in the College's Swigert Commons.
Kate Gilmore received her MFA (2002) at the School of Visual Arts, New York and BA (1997) at Bates College, Lewiston, Maine. She currently has a solo exhibition at Franco Soffiantino Arte Contemporanea, Turin, Italy, and last year, at the Institute of Contemporary Art, Philadelphia, University of North Texas Art Gallery, Denton, Texas, Artspace San Antonio, San Antonio, Texas, Maisterravalbuena Galeria, Madrid, Spain, and CESAC Centro Sperimentale Per Le Arti Contemporanee, Caraglio, Italy. She is represented by Smith-Stewart Gallery in New York.
MFA In Visual Studies Lecture | Kate Gilmore
12:30 p.m. Thursday, October 15 | PNCA Main Campus Building, Swigert Commons, 1241 N.W. Johnson St.
Photo courtesy of the artist.
International Writing Program Engages with PNCA Campus
For one dynamic week, PNCA's campus was enlivened by the work and insight of five international writers from University of Iowa's "International Writing Program":http://iwp.uiowa.edu/index.html.
The five writers included: Fflur Dafydd, a novelist, literary critic and musician from Wales; Soheil Najm, a poet from Iraq; Osman Conteh, a novelist from Sierra Leone; Fedosy Santaella, a fiction writer from Venezuela; and Lijia Zhang, a nonfiction writer from China.
Along with engaging the community during a free and public lecture on October 5 at the College, the group participated in a range of literary events in the Portland area, including "Wordstock":http://www.wordstock.com.
PNCA would like to thank PNCA faculty member and writer-in-residence Barry Sanders for organizing the visit and the community as a whole for participating.
Faculty Member Nan Curtis and Alumnus Nicolaii Dornstauder '09 Reviewed in Artforum
Work by faculty member Nan Curtis and MFA in Visual Studies alumnus Nicolaii Dornstauder '09 was recently reviewed on "Artforum.com":http://artforum.com/picks/section=us&mode=past#picks23903. Curtis and Dornstauder are currently featured in a two-person show at "Fourteen30 Contemporary":http://fourteen30.com/ gallery in SE Portland.
Fourteen30 Contemporary is one the many Portland galleries featuring PNCA artists this October.
PNCA the Focus of OPB's Oregon Art Beat
In honor of the PNCA's Centennial academic year, the College is the focus of Oregon Art Beat's first episode of the season, airing October 8. Former faculty member "Lucinda Parker '66":http://www.pnca.edu/exposure/stories/18/embodying-exuberance, current BFA faculty member "Paul Missal":http://www.blackfish.com/Artist-Detail.cfm?ArtistsID=17 and alumnus "Ty Ennis '06":http://www.newamericanartunion.com/Artists/Ty_Ennis/ are featured.
"Watch the episode online now.":http://www.opb.org/programs/artbeat/episodes/view/1101
Sloppy Craft Features Anne Wilson and Panelists
A term coined by Anne Wilson at the School of the Art Institute of Chicago, "sloppy craft" is described by craft theorist Glenn Adamson as the "unkempt" product of a "post-disciplinary craft education."
Join Anne Wilson, Wilson's former student Josh Faught (University of Oregon), Nan Curtis (Pacific Northwest College of Art), local artist Jessica Jackson Hutchins and Namita Gupta Wiggers to discuss this rising—and controversial—approach to conceptual craft practice.
This conversation takes place in preparation for a related exhibition co-curated by Josh Faught, assistant professor and program coordinator of fibers, University of Oregon; and Namita Gupta Wiggers, curator, Museum of Contemporary Craft. Sloppy Craft is scheduled to exhibit at the Museum during the 2010–2011 exhibition season.
Lecture | Craft Dialogue: Sloppy Craft
Featuring Anne Wilson
1–3 p.m. Saturday, October 10
PNCA Main Campus Building, Swigert Commons, 1241 N.W. Johnson St.
Portland Galleries Celebrate PNCA
During October, Portland's vibrant gallery scene overflows with artists from the PNCA community including students, alumni, faculty and faculty emeriti. Thank you to all of the galleries for their continued support and for celebrating PNCA's Centennial, 1909-2009.
For complete show information; please contact individual galleries.
Download the "PNCA=100 Inside/Outside—All Around Town brochure":http://homeroom.pnca.edu/download/585127.
*23 Sandy Gallery*
623 NE 23rd Ave
Group Show: "Broadsided! The Intersection of Art and Literature"
Artist: Malini Gupta '05
*Augen Gallery*
716 NW Davis St
Group Show: "Honoring PNCA Alumni"
Artists: George Johanson '50; Sally Cleveland '81; Terry Waldron '92; Morgan Walker '93; Jef Gunn; Stan Wood '71
*Beppu Wiarda Gallery*
319 NW Ninth Ave
"New Work–Mixed Media on Canvas"
Artist: Deborah Gillis '95
*Blackfish Gallery*
420 NW Ninth Ave
Group Show: Work by PNCA Alumni & Faculty
Artists: Lynda Ater '01; Barbara Black '84; Tori Bryer Board Member; Jana Demartini, Former Faculty; Paul Missal, Faculty; Angela Passalacqua '88; Eric Rue '05; Sue Tower '82; Judith Wyss '81
*Butters Gallery*
520 NW Davis St
"PNCA at 100: Jeffrey Butters"
Artist: Jeffery Butters '90
*Charles A Hartman Fine Art*
134 NW Eighth Ave
"Hayley Barker: Chimeras"
Artist: Hayley Barker, Faculty
*Elizabeth Leach Gallery*
417 NW Ninth Avenue
"Written from Exile"
Artist: Ryan Pierce, Faculty
*Fourteen30 Contemporary*
1430 SE Third Ave
"Nan Curtis + Nicolaii Dornstauder: In conjunction with the Centennial Celebrations of PNCA"
Artists: Nan Curtis, Faculty; Nicolaii Dornstauder '09
*Froelick Gallery*
714 NW Davis St
"Tom Prochaska & Claudio Tschopp"
Artist: Tom Prochaska, Faculty
*Launch Pad Gallery*
534 SE Oak St
Group Show: "Family"
Artists: Justinian Frey '94; Carolina Medina-Dupaix '04; Jason Kappus
*Laura Russo Gallery*
805 NW 21st Ave
Group Show: "PNCA Alumni"
Artists: Jay Backstrand '61; Marlene Bauer '76; Michael Brophy '85; Louis Bunce, Faculty Emeritus; Sean Cain '94; Tom Cramer '82; Chris Gander '86; William Givler, Former Dean; Roll Hardy '02; Manuel Izquierdo '51, Faculty Emeritus; Jackie Johnson '74; Mary Josephson '86; Lucinda Parker '66; Jack Portland '71; Jan Reaves; Eric Stotik '85; Margot Voorhies Thompson '84; Sherrie Wolf '74
*Museum of Contemporary Craft–Exhibition Space*
724 NW Davis St
Group Show: "Call + Response"
Artists: David Eckard, Faculty; Anne Marie Oliver, Faculty
*NEMO Headquarters*
1875 SE Belmont St
"Off the Clock"
Artist: Andrea Paustenbaugh '06
*Onda Gallery – Lake Oswego*
Group Show: "Celebrating Art and Nature - National Wildlife Refuge Week Kickoff"
Artist: Susan Jensen '81
*PNCA Hybrid Gallery*
indigo @ twelve | west; 430 SW 13th Ave
Group Show: "Possible Progress in Body Relations"
Artists: Alexander Ian Smith '11; Chad Hinman '10; Taryn Tomasello '12; Lacy Davis '10; Robert Burns '10; Sokhun Keo '10; Sarah Johnson '09; and Brennan Broome '11
*PDX Contemporary Gallery*
925 NW Flanders St
"Across the Hall: In Honor of PNCA @ 100"
Artist: Derek Franklin '09; Molly Vidor '94
*Pulliam Gallery*
929 NW Flanders St
Artists: Kay French '86; Linda Hutchins '88; George Johanson '50; Paula Overbay '75; Yoshihiro Kitai, Faculty; Raul J. Mendez '97; G. Lewis Clevenger '73
*Tribute Gallery at Everett Station Lofts*
328 NW Broadway #117
"Se(xxy) Plastic"
Artists: Adrienne Butzer '07, Seth A. Leamer '07
*Mark Woolley and ANKA Gallery*
325 NW Sixth Ave
"All in the Family"
Artists: Melinda Thorsnes '71; Diane Avio-Augee '80; Emery Hinkley '90; Brigitte Dortmund '92; Nancy Franzino; Miles Cleveland Goodwin '07; Julia Stoops; Stuart Cornell '97; Tamara English '04; Ed King '04; Rachel Denny '97; Michael T. Hensley '95; Rachael Allen '03
PNCA the Focus of OPB's Oregon Art Beat October 8
In honor of the PNCA's Centennial academic year, the College is the focus of Oregon Art Beat's first episode of the season. Former faculty member "Lucinda Parker '66":http://www.pnca.edu/exposure/stories/18/embodying-exuberance, current BFA faculty member "Paul Missal":http://www.blackfish.com/Artist-Detail.cfm?ArtistsID=17 and alumnus "Ty Ennis '06":http://www.newamericanartunion.com/Artists/Ty_Ennis/ will be featured. View the episode Thursday, October 8 at 8pm on OPB TV. Episode repeats Sunday, October 11 at 6pm. "Read more.":http://www.opb.org/programs/artbeat/episodes/view/1101
MFA in Visual Studies Presents Martin Kersels
The MFA in Visual Studies program presents Los-Angeles-based artist Martin Kersels in a free, public lecture at 6:30 p.m. Thursday October 8, part of PNCA's Graduate Visiting Artists Lecture Series.
Los Angeles-based artist Martin Kersels has long used art as a forum for wrestling with the limitations and possibilities of being a fully embodied, sentient and emotional being.
He employs a range of media, from sculpture and photography to video and performance. He has worked on staff at the Los Angeles County Museum of Art, the UCLA Art Department, and currently serves as a faculty member and co-director of the art program at the California Institute of the Arts.
Kersel's work has been featured in exhibitions at the Institute of Contemporary Art in Philadelphia, the J. Paul Getty Museum and ACME in Los Angeles, Deitch Projects in New York, and the Galerie Georges-Phillipe and Nathalie Vallois in Paris. His pieces are in the public collections of the Los Angeles County Museum of Art and the J. Paul Getty Museum in Los Angeles, La Reina Sofia in Madrid, and the Musée National d'Art Moderne Centre Pompidou in Paris.
MFA in Visual Studies Lecture | Martin Kersels
6:30 p.m. Thursday, October 8 | The Lab at Museum of Contemporary Craft, 724 N.W. Davis St.
Etsy Participates in PNCA–CYAN/PDX Cultural Residency Series
PNCA welcomes Etsy's Matt Stinchcomb for the PNCA-CYAN/PDX Cultural Residency Series, including a public lecture and a range of interactions with the PNCA community.
Portland's DIY culture continues to be recognized internationally as a central hub of the American DIY movement, celebrated as a collective of creative talent with a progressive approach to the entrepreneurial spirit. Presented by the PNCA–CYAN/PDX Cultural Residency Series.
Salon | Digitally Building the DIY Community
Buy, Sell, and Live Handmade
A Conversation with Matt Stinchcomb of Etsy
5:30 p.m. Wednesday, October 7
CYAN/PDX, 1720 S.W. Fourth Ave.
A Gerding Edlen Project with Pacific Northwest College of Art. Part of the PNCA– CYAN/PDXCultural Residency Series.
MFA in Visual Studies Lecture: Ward ShelleyBrooklyn-based artist Ward Shelley delivers the first lecture presented by the MFA in Visual Studies program. Shelley specializes in large-scale projects that freely mix sculpture and performance. Over the last five years, Shelley has concentrated on bizarre functioning architectural pieces in which he lives and works during the exhibition, monitored with live surveillance video equipment. His works are in the collections of the Museum of Modern Art, New York, the Brooklyn Art Museum, and The Elizabeth A. Sackler Center for Feminist Art. This lecture took place at Museum of Contemporary Craft on September 24, 2009. Listen to a Podcast of "Ward Shelley":http://homeroom.pnca.edu/download/585576.
PNCA Hosts University of Iowa International Writing Program
This year for the first time PNCA will be hosting five writers through the International Writing Program, which since its founding in 1967 at the University of Iowa, has brought together more than 1,000 rising and established literary stars from 120 countries to develop a community of writing to enhance international understanding.
The writers' week at PNCA is organized by PNCA faculty member and writer-in-residence Barry Sanders and includes both immersion in the campus curriculum along with collaborative public programming. The experience provides writers from around the world with a sense of American innovation and openness, and exposes U.S. readers, writers, and artists to international perspectives on the art and process of writing.
Participating writers include: Fflur Dafydd, a novelist, literary critic and musician from Wales; Soheil Najm, a poet from Iraq; Osman Conteh, a novelist from Sierra Leone; Fedosy Santaella, a fiction writer from Venezuela; and Lijia Zhang, a nonfiction writer from China.
Don't miss the first event, hosted by PNCA:
Reading + Reception | 6:30 p.m. Monday, October 5
PNCA Main Campus Building, Swigert Commons, 1241 N.W. Johnson St.
Download the "International Writing Program at PNCA schedule (PDF)":http://homeroom.pnca.edu/inline/584932.pdf for a full list of events and visiting writers' bios.
MFA in Visual Studies Presents Artist Ward Shelley
Join PNCA's MFA in Visual Studies Program for the kickoff of its lecture series with an intimate lecture by artist Ward Shelley, 6:30 p.m. Thursday, September 24 in the Lab at the Museum of Contemporary Craft, 724 N.W. Davis St.
Brooklyn-based artist Ward Shelley specializes in large-scale projects that freely mix sculpture and performance. Over the last five years, Shelley has concentrated on bizarre functioning architectural pieces in which he lives and works during the exhibition, monitored with live surveillance video equipment.
Shelley also works with the collaborative artist group BBS and talented young artists such as Douglas Paulson and Alex Schweder, with whom he realized the monumental Flatland project at New York's SculptureCenter in 2007. His works are in the collections of the Museum of Modern Art, New York, the Brooklyn Art Museum, and The Elizabeth A. Sackler Center for Feminist Art.
MFA in Visual Studies Lecture | "Ward Shelley":http://www.wardshelley.com/
6:30 p.m. Thursday, September 24 | The Lab at Museum of Contemporary Craft, 724 N.W. Davis St.
Craft Conversation: David Eckard and Anne Marie Oliver
Don't miss an exciting opportunity to discuss the dynamic work included in the Museum of Contemporary Craft's exhibit Call + Response with PNCA Faculty David Eckard and Anne Marie Oliver, 1 p.m. Saturday, September 26 at the Museum, 724 N.W. Davis St.
Anne Marie Oliver, Assistant Professor of Intermedia and contemporary art theory, Pacific Northwest College of Art, and David Eckard, Chair of the Sculpture Department and Instructor in Foundation and Intermedia departments, Pacific Northwest College of Art will discuss the collaborative process they engaged in for Call + Response.
Drawing on the musical concept of "call and response," this project provides a rare platform for artists and art historians to engage with each with other in vibrant conversation. A multi-layered exhibition, Call + Response features diverse works by eight pairs of art and art history faculty members from colleges and universities who have taught in Oregon for roughly ten years or less.
Through multimedia content, contextual writing, the presentation of studio works and public programs, this intriguing project celebrates and provokes the recent influx of ideas brought to Oregon by these faculty members – who are breaking ground within their fields nationally and internationally. In effect, Call + Response considers what role these individuals, and their institutions, play within the state's shifting creative landscape.
Learn more about Call + Response at the exhibition microsite:
Committed to the advancement of craft since 1937, Museum of Contemporary Craft is one of Oregon's oldest cultural institutions. Centrally located in Portland's Pearl District, the Museum is nationally acclaimed for its curatorial program and is a vibrant center for investigation and dialogue, expanding the defi nition of craft and the way audiences experience it. Visit the Museum online at www.MuseumofContemporaryCraft.org.
Special Event | Craft Conversation: David Eckard and Anne Marie Oliver
Saturday, September 26, 1 p.m. | Museum of Contemporary Craft, 724 N.W. Davis St.
Printmaker Zha Sai Exhibits New Work
Chinese printmaker Zha Sai exhibits her woodcuts in an exhibition entitled "Rhythm of the Shadows" at PNCA's Print/Photo Gallery from September 21 through October 9. Image: The Sway of the Shadow, 2006, reduction woodcut, 11.4" x 11.4"
Her work is strongly influenced by the natural environment. She lives in Wuhan, the capital of the Hubei Province in Central China. Although it is a large city with a population of nearly 14 million people, it is surrounded by forests and lakes and is still known as "The Place of a Thousand Lakes". She is also influenced by music, especially the music of Antonín Dvořák. From these inspirations she has made compelling work in the form of reduction woodcuts.
Zha Sai comes to the Northwest under the auspices of Art Partners, a two-artist collaboration comprised of Seattle artists Cathe Gill and Barbara Pitt that has promoted artist exchanges since 1999 with artists from China, Ireland, the Czech Republic, Slovakia and Kuwait. Her exhibition here is an initiative of the Printmaking Department at PNCA.
Lecture | Zha Sai
12:30 Monday, October 5
PNCA Main Campus Building, Swigert Commons, 1241 N.W. Johnson St.
Memorial for Manuel Izquierdo
A public memorial will be held in honor of former Faculty Emeritus Manuel Izquierdo on Saturday, September 26, from 4-6 p.m. at the Pacific Northwest College of Art, Swigert Commons, 1241 N.W. Johnson Street in Portland.
All are welcome to attend.
Learn more about "Manuel Izquierdo":http://www.pnca.edu/exposure/news/649/remembering-pnca-faculty-emeritus-manuel-izquierdo.
Read the Oregonian's "Manuel Izquierdo, 1925-2009: an appreciation":http://www.oregonlive.com/O/index.ssf/2009/07/manuel_izquierdo_19252009.html by D.K. Row.


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Topic: Habitats
Friday, September 7, 2012
The Earth's natural energy has been fueling ecosystems around the world for billions of years. What are some ways that we can tap into this renewable resource?
Topics Covered: Question Power, geothermal, Geothermal Energy, Watt, energy, Watt Wall, Global, Measuring Energy, power, Heat, earth, resources, RVA, Data, stem, science, technology, engineering, Math, Mathematics, Scientific Method, Force, Motion, Earth Resources, Earth Science, Phsyics, Earth Patterns, Cycles, Change, Habitats, gas, numbers, fuel, green, eco, renewable, Money, Planning, Accounting, Population, future


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There are 46 results - Show results on a map Print this page
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Very well located on Melvill Road, and with off-road parking, Eden Lodge boasts comfortable rooms and a swimming pool. The friendly hosts serve dinner by arrangement and do all they can to ensure a co...More about Eden Lodge
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Search for Captain Walbridge Called Off in HMS Bounty Sinking
The search has been called off for Captain Robert Walbridge of the HMS Bounty which sunk on Sunday, October 29th. He joins Claudine Christian as the two casualties from the Bounty's sinking. Captain Walbridge had captained the HMS Bounty for the last 17 years and, by all accounts, had grown quite attached to the ship. While the crew of the Bounty refused to speak about its sinking, Captain Walbridge's deep love for the tall ship most likely played a role in her sinking. What is known is that Captain Walbridge felt that in tough weather a ship stands a better chance of survival at sea rather than in port–a commonly held bit of conventional wisdom amongst sailors.
Many people are questioning why and how the HMS Bounty came to be at sea during a hurricane. Since the crew is not talking at this time, all one can do is speculate, but I believe that the story that will most likely emerge is that the Bounty was in dock in North Carolina and believed to be safely out of harm's way. However, as the superstorm developed and widened, it became apparent that the Bounty ran the risk of being smashed to pieces by the storm while in harbor. For the crew of the Bounty, the ship represented their unique livelihood. There simply weren't many tall ships to crew in the modern world. And so the captain and crew's concern for their ship had layers both of love and of commerce.
At some point the captain no doubt weighed the ship's chances of survival by staying in dock or taking to sea. The plan was then made to attempt to take the Bounty to sea and sail around the developing storm. Unfortunately, the 15-foot waves eventually overwhelmed the ship and then a distress call was sent out. The captain's priority was to get his crew safely into the lifeboats. Accounts from the sinking state that Claudine and Captain Walbridge were the last on board and were washed overboard while attempting to get into the lifeboats.
In many ways the decisions facing the captain and crew of the HMS Bounty were typical of those facing sailors constantly. The risks of death on the job are quite real, as was depicted in the movie about another superstorm that caused the loss of a ship and crew, the Andrea Gail in the movie "The Perfect Storm." These decisions are made with some frequency, and the vast majority of the time they work out well. It's only when these decisions go wrong that we get to ask "why on Earth were they out there in the first place?" from the safety of our living rooms.
One of the factors that the influence these decisions is simply the love of the ship on behalf of the captain and crew and their desire to protect her from harm. But if ever there were a case to help us understand their love, it must be the case of the one of a kind tall ship, the HMS Bounty.