text:
PG-13 | | Drama, History
A Salem resident attempts to frame her ex-lover's wife for being a witch in the middle of the 1692 witchcraft trials.
22 September 2006 | sugar_n_spice
10 | I am not someone to randomly give out a perfect score for a movie...
...And I also happen to be a very critical person of most films. With that being said, The Crucible completely blows me...
Jennifer Love Hewitt was considered for the role of Abigail Williams.
Judge Danforth:
The pure in heart need no lawyers.
In the scene where John Proctor goes out into the pond, when the camera cuts back to the crowd onshore, an airplane condensation trail can clearly be seen in the sky.
$62,995 (USA) (29 November 1996)
$7,340,103 (USA) (21 February 1997)


text:
5 things to do in Teluk Intan
Teluk Intan is a small town that is just 2-hours away from Kuala Lumpur. Once known as Teluk Anson, (Anson Bay) it was named in honour of Major-General Sir Archibald Edward Harbord Anson who planned for its modern township in 1882. A century later in 1982, the name was finally cemented as Teluk Intan which […]
All You Need To Know (And Other Stuff) About Malaysian Airports
By Lloyd Green Travelling to Malaysia soon? Well, you're bound to stopover at one of it's many airports. If you're nervous about flying, are a stickler for safety and cleanliness, or just interested in random facts about planes, here's a list of some useful (or not so useful) information that will help make your trip […]
What do expats say about Malaysia?
By Emma Richards Well, depending on where you come from, 'Gosh, it's hot!' is quite often the first thing any expat will say about Malaysia. Stepping off the plane for the first time, feeling the humid air fill my lungs and the stifling warmth envelope me as I emerged from the cool into the tropical […]
Where Kids Rule
DARYL YEP CHECKS OUT KIDZANIA, THE NATION FOR KIDS, AND IS FASCINATED BY THIS AWARD-WINNING THEME PARK Kai! That's probably the first word you'll hear as you step into KidZania, Kuala Lumpur's latest indoor family edutainment centre. Here's how greetings are exchanged instead of hello. It's personal; giving kids an instant sense of belonging and […]
Putrajaya, the making of a city
As a young capital, Putrajaya may not have the character and soul of the great cities of the world, but it is well on its way there with innovative architecture, community-centric town planning and long term ambitions. In relation to many of Malaysia's other cities like Kuala Lumpur and Melaka, the garden city of Putrajaya is like a new kid on the old block. Granted, it lacks the dramatic history of the former and the age-old culture of the latter but what it has in excess is youthfulness, a modern vision and a spirit to embrace the new.
A Hundred Years in Ipoh
Being married to a man from Perak, it was inevitable that as I learned to love this person, I also had to learn to love his home state. Fortunately, neither task was too difficult to accomplish!
Perak has been fantasized, romanticized and idealized by all sorts of people from all walks of life throughout Malaysian history. In the days when tin prices were sky high, the Kinta Valley in Perak, possessing the world's richest alluvial tin deposits, held promise of great fortunes for already-wealthy businessmen, small-time speculators and the average dreamer.

subset: commoncrawl


text:
Local Sports
Cards Top Cubs After Big Ninth
7/15/2013 @ 8:48 AMCHICAGO (AP) - Yadier Molina hit a three-run homer in St. Louis' four-run ninth inning, and the Cardinals beat the Chicago Cubs 10-6 on Sunday night in the majors' final game before the All-Star break.
Allen Craig lined a tie-breaking RBI single into left field before Molina drove an 0-2 pitch from Kevin Gregg over the wall in left for his seventh homer. Craig and Molina tuned up for Tuesday's game in New York with four hits apiece, helping St. Louis to a season-high 21 hits overall.
Chicago went up 4-3 on Darwin Barney's three-run homer in the sixth and pinch hitter Cody Ransom had a tying two-run double in the eighth, but the Cubs' bullpen was unable to hold off the NL's highest-scoring team.
See other LocalSports news:RLC Softball Advances to Final Four
Werth's bat, Ross' arm lead Nationals past Cardinals
Kauling Leaving Centralia For Wesclin
Witzel Steps Down At Woodlawn
Holst To Coach One More Time


text:
My aim is always to help our readers understand how to evaluate tools within the context of their particular needs and goals. One of the ways we do that is through EContent's annual Trendsetting Products list. Each year, we turn to our inboxes, our coverage, and to our contributors to find out what products are helping content creators of all kinds stay on top of their game. I hope that you find these tools as interesting and as potentially helpful as I do!
Oct 14, 2010
IP video technology developer VBrick Systems announced a partnership with LifeSize, a division of Logitech, to extend high-definition IP video delivery within the enterprise. The combined VBrick Systems and LifeSize solution enables capabilities that neither IP video streaming nor video conferencing provide alone. The partnership translates into major advantages in the form of increased participation in content creation, scalable distribution of content across the enterprise and over the internet.
The combination of VBrick VEMS and LifeSize Video Center solution allows organizations to deliver a wide variety of IP video applications, from executive and event broadcasts to training and education sessions, digital signage, webcasting, distributing television content, or surveillance. The content can also be viewed using a PC, Mac, mobile device, VBrick IP Set Top Box or Digital Signage player.
The combined LifeSize and VBrick Systems capabilities is expected to be available in the coming months.
(www.vbrick.com, www.lifesize.com)


text:
Kevin Spacey may be about to come out
Post your comment
Comments on this article are now closed.
Reader comments
Thanks for the correction.
Give it a rest please. Did it ever occur to anyone that Mr. Spacey doesn't talk about his sex life because he's a gentlemen and doesn't want to embarrass his lady friends? Gentlemen don't; hounds do.
What a person does in their bedroom is their busness, as long as he/she has never done anything to hurt someone intentionally. So I would suggest to you, do yourself a favour and get a real job,one in which your family can be proud of your job discription instead of aspersing comments about someone who has never done you any harm – Remember bad Karma!
These comments are un-moderated and do not necessarily represent the views of PinkNews. If you believe that a comment is inappropriate or libellous, please contact us.
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Search for "Warrior Queen" on Amazon.com
The story of Chesley Sullenberger, who became a hero after gliding his plane along the water in the Hudson River, saving all of his 155 passengers.
A man's attempt at a peaceful, quiet family vacation in the Caribbean is marred by the chatty guy living next door.
An American attorney loses her job and finds work as a housemaid in England.
Life for New England realtor Hildy Good begins to unravel when she hooks up with an old flame of hers from New York. Based on Ann Leary' 'The Good House.'
Two detectives look to solve a series of armored car robberies; the one hitch being the one who catches the crooks will receive a promotion.
The story of Green Bay Packers coach Vince Lombardi and how he lead his team to victory during the 1967 National Football League Championships. Based partly on the memoir "Instant Replay" by team member Jerry Kramer and Dick Schaap.
A business mogul puts his career aside to run his wife's presidential campaign.
A young lawyer joins forces with a veteran attorney to help exploited migrant workers in Southern Florida's sugar industry.
A mob hit man recalls his possible involvement with the slaying of Jimmy Hoffa.
A look at the life of an aging insult comic.
Find showtimes, watch trailers, browse photos, track your Watchlist and rate your favorite movies and TV shows on your phone or tablet!


text:
The Disney Marine Role-Playing Faire attraction, which recently celebrated its disguised anniversary
This is the place to hang out if you have a marine-related disorder. Here you can drool at marine clothes, read along with marine anecdotes, take a "How to Be a Marine" class, buy limited-edition marine baubles, and be bitten by an in-the-flesh marine!
Run from Princess Mode
© copyright 2011–2016
A Pants Aflame production


text:
Geoacoustic inversion by mode amplitude perturbation
Poole, Travis L.
MetadataShow full item record
Citable URIhttp://hdl.handle.net/1912/1584
This thesis introduces an algorithm for inverting for the geoacoustic properties of the seafloor in shallow water. The input data required by the algorithm are estimates of the amplitudes of the normal modes excited by a low-frequency pure-tone sound source, and estimates of the water column sound speed profiles at the source and receiver positions. The algorithm makes use of perturbation results, and computes the small correction to an estimated background profile that is necessary to reproduce the measured mode amplitudes. Range-dependent waveguide properties can be inverted for so long as they vary slowly enough in range that the adiabatic approximation is valid. The thesis also presents an estimator which can be used to obtain the input data for the inversion algorithm from pressure measurements made on a vertical line array (VLA). The estimator is an Extended Kalman Filter (EKF), which treats the mode amplitudes and eigenvalues as state variables. Numerous synthetic and real-data examples of both the inversion algorithm and the EKF estimator are provided. The inversion algorithm is similar to eigenvalue perturbation methods, and the thesis also presents a combination mode amplitude/eigenvalue inversion algorithm, which combines the advantages of the two techniques.
Submitted to the Department of Mechanical Engineering in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Applied Ocean Sciences at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution February 2007
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Baby First TV Shows
The Baby First channel is a station that provides a huge resource for parents that may be interested in helping their toddlers learn new concepts. The channel has a plethora of different shows that will entice the young minds of babies. Parents that want to stimulate their children with cartoons and shows that are designed specifically for children will appreciate this channel.
It is a station that provides a large number of shows that are designed for children between the ages of 1 to 5. The shows here provide many avenues for learning for children that may be interested in music. Parents can tune in and help their children learn about numbers and words with these different shows. The various cartoons will also provide an opportunity for children to learn about things like shapes, sounds and colors.
This is a channel that is designed to help parents that want to help their children with cognitive skills at an early age. This channel has become a resource that some teachers will use in classrooms to improve problem-solving skills. The shows found on this channel will be able to help children build a foundation in basic numbers and words before attending school. The channel provides shows that will give many toddlers a head start. The channel provides a learning platform in a fun way. It is a show that manages to merge a number of shows the promote learning. The shows here also encourage children to use their imagination and become creative.
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text:
Super Nintendo Games
Super Mario RPG: Legend Of The Seven Stars Questions for Super NintendoHome / Super Nintendo /
Game Questions & Answers (Q&A) service lets you ask questions about video games for game consoles or PC games. So ask your Super Mario RPG: Legend Of The Seven Stars question for Super Nintendo and get answers from real gamers or answer questions and share your insights and experience with the rest of the gaming community.
Boxshot & Details
- Developer: Squaresoft
- Publisher: Nintendo
- Genre: Role-Playing Console-style RPG
- Release: May 13, 1996
- ESRB: Not Set
Sponsered Links
Stuck in this game? Set a specific, clearly defined question and let other gamers answer your question or view answers to previously asked questions. If you think you are an expert then please try to help others with their questions.
The arrow boss in the forest maze
From: Hamoudy
From: Hamoudy
Total 1 Answers
Posted On: 08-04-2012
Posted On: 08-04-2012
How to beat capitain culamari
From: the real gamer
From: the real gamer
Total 0 Answers
Posted On: 06-04-2012
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Getting pass marry more
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From: ash
Total 1 Answers
Posted On: 02-03-2012
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Super Mario RPG: Legend Of The Seven Stars - Video Walkthrough - Part 1 of 2
- Aug 3, 2014, Views: 111
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- Aug 3, 2014, Views: 107
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- Aug 3, 2014, Views: 120
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Games You May Like


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Results 4991 — 5,000
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Web Design and Devloper Internship
goGetter Growth Partners — Fairlawn, OH
We are a full-service advertising agency that is seeking a Web Devloper Intern. This individual should be majoring in Computer Science, Web Development, Graphic Design or similar. You will be asked to…
Application Deadline: Available Year-round
Promotions/Event Internship
Townsquare Media Inc. — Grand Rapids, MI
If you're enthusiastic about the radio and digital media industries, Townsquare Media might be the right place for you. You'll get hands-on experience and have fun in the process. Are you responsible?…
Application Deadline: Available Year-round
Community Manager (Females Only)
iGirl Tech News — Virtual
iGirl Tech News is a brand new online media platform that empowers, inspires, and supports females in technology with 100% female created content. Our mission is to significantly increase the female v…
Application Deadline: Available Year-round
Research Department Internship
NephCure Kidney International — King of Prussia, PA
NephCure Kidney International (NKI) is the only organization committed to supporting research seeking the cause of the potentially debilitating kidney disease Focal Segmental Glomerulosclerosis (FSGS)…
Application Deadline: Available Year-round
Community-Focused Real Estate Project to help Economically Empower Artists and Community Residents
ArtCondo — New York, NY
ArtCondo (www.ArtCondo.com) is a community-focused real estate initiative that helps creative groups purchase property collectively, in partnership with neighborhood residents. ArtCondo's mission is t…
Application Deadline: Available Year-round
Editorial Internship
EmpowHER — Virtual
Description: EmpowHER.com, an award-winning womens health website based out of Scottsdale, is looking for an editorial intern to join their team. As an editorial intern, you will have the opportunity …
Application Deadline: Available Year-round
Community Internship
EmpowHER — Virtual
Description: EmpowHER.com, an award-winning womens health website based out of Scottsdale, is looking for a community intern to join their team. As a community intern you will help grow EmpowHERs user…
Application Deadline: Available Year-round
Tech Writers
29thTech — Virtual
We are an established iOS blog looking for journalism students with a keen interest in technology, specifically Apple products. If you are passionate about writing and are able to tell a good story wi…
Application Deadline: Available Year-round
Branding and Image Consulting and Strategy Intern
Dolce Belletto — Virtual
Dolce Belletto is a boutique image consulting and personal branding firm located in Nashville, TN. We specialize in brand development, brand management, brand recognition, brand cohesiveness and visua…
Application Deadline: Available Year-round
Gallery Assistant Internship
ZuCot Gallery — Atlanta, GA
ZuCot Fine Art Gallery is located in the Castleberry Hill District of Atlanta. We are flexible with the days the Intern would like to work and require 8-20 hours a week for at least 3 moths. This can …
Application Deadline: Available Year-round


text:
By Tolly Taylor and Shane Monaghan
Ryan Pace and the Chicago Bears front office were not apprehensive during the 2016 NFL Draft. The team made multiple trades en route to drafting nine players, seven of which were defenders.
ESPN NFL staff writer Matt Bowen spoke with Medill Reports about why he felt the Bears addressed numerous needs and became faster and more physical on defense. Continue reading


text:
Setting: Egyptian
Group DescriptionGames that either take place, partially or entirely, in ancient Egypt or a fantasy country with a strong resemblance to Egypt, featuring typical elements of the Egyptian culture. Might contain supernatural themes, usually based on Egyptian legends.
Note: Games set in Egypt after it was conquered by the Arabs in 7th century do not belong to this group, and should be part of the Middle Eastern one.
|Viewing Page 1 of 8
|(items 1-25 of 179)
Selected Covers
Front cover for Cleopatra's Fortune
Front cover for Pyramid of Doom
Front cover for Tomb of Syrinx
Front cover for Fighting Warrior
Front cover for Crypts of Egypt
Selected Screenshots
Screenshot from Cleopatra's Fortune
Screenshot from Pyramid of Doom
Screenshot from Tomb of Syrinx
Screenshot from Fighting Warrior
Game group created by chirinea (36304), additional games added to group by Spenot (8437), Cantillon (13784), marley0001 (1413), Rola (7752), Patrick Bregger (151087), POMAH (36913), Slik (888), Corn Popper (69507), piltdown_man (104860), Ƒreddƴ (5673), Heliophage (351), Victor Vance (6051), Flapco (5689), Sciere (358002), Daedolon (3268), Depeche Mike (17187), Kabushi (153766), Opipeuter (16493), jaXen (25428), vileyn0id_8088 (15572), Scaryfun (13306), Multimedia Mike (18029), BdR (4375), JudgeDeadd (14925), LepricahnsGold (115048), Yearman (19607), vedder (20584), Jeanne (75483), Big John WV (25494) and Macs Black (77759)
Screenshot from Crypts of Egypt
Screenshot from Crypts of Egypt


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text:
|Name: _________________________
|Period: ___________________
This quiz consists of 5 multiple choice and 5 short answer questions through "Black Bark in Winter".
Multiple Choice Questions
1. What happens to the mother bear that is attempting to protect her cub from Inman?
(a) Inman is forced to kill her.
(b) She simply walks away, leaving her cub behind.
(c) She is killed by a passing Indian.
(d) She charges him and falls from a cliff.
2. What happens to Veasey and Inman after they leave the farmer's home?
(a) They are shot by the Home Guard.
(b) They are put in jail.
(c) They are forced to pledge allegiance to the Union.
(d) They are placed in a new army regiment.
3. What map does Inman follow on the next leg of his journey in the chapter, The Doing of It?
(a) A map given to him by the yellow slave.
(b) A map given to him by the Federals.
(c) A map given to him by Lila.
(d) A map given to him by Veasey.
4. Why does Inman believe the person he sees at Deep River is up to no good?
(a) The person blushes when questioned.
(b) The person has a weapon.
(c) There is a person bundled up on the back of his horse.
(d) The person appears nervous and incoherent.
5. Who rides up and begins asking Strobrod and Pangle questions?
(a) Some Federals.
(b) An Indian scout.
(c) A Confederate scout.
(d) The Home Guard led by Teague.
Short Answer Questions
1. Why does Inman walk into town?
2. Who joins Ada and Ruby for supper as they enjoy what will most likely be their final dinner outside that fall?
3. What is the name of the spell Inman speaks after the fight he is forced into outside the settlement?
4. Why does Ada decide not to return to Charleston after her father's death?
5. Which of Teague's men argues that they need to bring in a living prisoner?
This section contains 359 words
(approx. 2 pages at 300 words per page)


text:
The Members of Chambers at 1kbw are delighted to announce the appointment of Nigel Scott to the new Chambers post of Chief Executive. Nigel comes to Chambers with a wide ranging background of military service, management consultancy, business development, executive … Continue reading
Category Archives: News
1kbw raises over £1,200 for Anthony Nolan Trust following Bake Off Competition
Last Friday evening saw members and staff eagerly await the outcome of the 2nd 1kbw Great Bake Off. A mix of enthusiastic efforts and win-at-all-costs entries faced the judges; His Honour John Altman, formerly Senior DFJ for London now an … Continue reading
Deborah Eaton QC and Stephen Jarmain attract huge turnout for seminar on Re. C presented by Resolution
Following the judgment on Re. C (Internal relocation), Deborah Eaton QC and Stephen Jarmain of 1kbw presented a very well attended seminar for Resolution. Robert George joined them in the presentation.
Last night's event, hosted by Mishcon de Reya, attracted … Continue reading
Richard Harrison QC, Madeleine Reardon, Jennifer Perrins & Katy Chokowry appear in landmark Supreme Court case concerning the habitual residence of children
The Supreme Court has allowed an appeal by the non-biological mother of a child who had been taken to live in Pakistan by the appellant's ex-partner, who was the child's biological mother. The Supreme Court allowed the appeal by a … Continue reading
1kbw's James Turner QC and Nicholas Anderson appear in Court of Appeal decision concerning the recognition and enforcement of overseas judgments under the Brussels II Revised Regulation
James Turner QC and Nicholas Anderson appeared before the Court of Appeal in the Matter of D (A Child) (International Recognition)
This appeal engaged fundamental principles concerning children's litigation and the full decision may be read here
Stewarts Law & 1kbw present joint seminar on internal relocation
A seminar was held on 12 January 2016 to consider the landmark case of Re C (Internal Relocation), in which Deborah Eaton QC and Stephen Jarmain instructed by Stewarts Law acted for the respondent mother.
The well attended event heard from … Continue reading
Philip Marshall QC, commences Chairmanship of the FLBA
Following his election in November 2015, Philip Marshall QC commenced his two year term as Chairman of the FLBA on 1 January 2016.
He is the latest in a long line of distinguished members and former members of 1kbw to … Continue reading
Deborah Eaton QC and Stephen Jarmain succeed in landmark Court of Appeal case clarifying the law on internal relocation
Judgment was today handed down in the case of Re C (Internal Relocation), in which Deborah Eaton QC and Stephen Jarmain instructed by Stewarts Law acted for the respondent mother.
The case concerned the law relating to the issue … Continue reading
His Honour John Altman on "ENE" in children cases
His Honour John Altman, Associate member of 1kbw and former Designated Family Judge for London, has written an article on Early Neutral Evaluation in disputes concerning arrangements for children, a service offered by the 1kbw DRS Unit. The Child … Continue reading
Supreme Court gives judgment in Re J (Morocco)
The Supreme Court has handed down judgment in Re J, the first ever case about the 1996 Hague Convention to reach the highest court.
James Turner QC acted on behalf of the Respondent mother. Richard Harrison QC acted on behalf … Continue reading


text:
I'm really beginning to think that I should turn in my Bengal's "fan card". it's more a casual card anyway.
Once again...no Buckeyes selected. You need a WR? Lets pass on Michael Thomas.
You want to draft a late round safety? Let's pass on Powell.
Oh...you want a big strong back...let's pick up Hill over Carlos Hyde. Nice to see how that worked out in the playoffs hehe.


text:
We expose slave masters and people smugglers in suburban Britain.
Is there more to life than money?
16 Oct 2012 18:54 GMT | Argentina, Ghana
The commander of Argentina's navy has resigned after the government punished two naval officers over Ghana's seizure of a three-masted tall ship over a dispute with creditors.
The resignation on Monday came in response to the "Libertad" training ship being retained in the Ghanaian port of Tema near the capital Accra since October 2.
A spokesperson from President Cristina Kirchner's office issued an abrupt statement on Monday to confirm that navy commander Admiral Carlos Alberto Paz had been replaced, without stating the reason for the decision.
A defence ministry statement said the former organisational chief of the navy, Alfredo Mario Blanco, was being investigated for changing the ship's itinerary, citing "operational reasons".
The navy's secretary general, Admiral Luis Gonzalez, was also suspended and is the subject of the investigation.
Earlier, two officers had also been suspended by the Argentine defence ministry over the decision to have the Libertad stop over in Accra with more than 300 people on board.
The vessel is being held due to investors filing a lawsuit in Accra to hold the Libertad as collateral pending payment of debts on Argentinian bonds which the government has defaulted on.
A supposed "vulture fund" called NML Capital had purchased Argentine bonds at a discount when the country's economy was in free fall in 2000.
NML Capital has since filed a claim in a Ghanaian court claiming it is owed more than $370 million, including the outstanding principle plus the interest. The majestic square-rigged ship used now by graduating Argentine naval cadets, established the world record in 1966 for a transatlantic crossing by a sailing ship.
According to media sources, in 2011 the ship specifically made stops in the Latin American region, a move aimed at a dodging any lawsuits over unpaid debt.
However in 2012 the ship toured Latin America, Europe and several ports in Africa before being seized in Ghana.
Alfredo Astiz, dubbed Blond Angel of Death, is accused of human rights abuses during 1976-1983 military rule.
Politics, Argentina
Some say they extort money, but franeleros insist they are just trying to make a living in Mexico's informal economy.
Will a plastic bag levy be enough to fix Indonesia's rubbish problem?
For ethnic Hausa, the traditional form of boxing known as dambe is more than a sport - it is part of their identity.
Nigeria, Sport
Rape is Malawi's most reported crime. Ujamaa initiative is helping schoolkids defend themselves against perpetrators.
Malawi, Africa, Women
Civil defence says clinic in Aleppo hit by air strike, the second time this week a medical facility has been targeted.
Syria's Civil War, Middle East, War & Conflict
UN humanitarian chief laments situation in Syria, where people are facing "appalling" desolation, hunger and starvation.
War & Conflict, Humanitarian crises, Middle East, Europe, Syria's Civil War
A Pakistani cotton farmer travels to Dubai to make his fortune but soon discovers he wants more from life than money.
Pakistan, Immigration, Dubai
The South African opposition leader says the EFF would fight if the ANC responded violently to peaceful protests.
Julius Malema, South Africa, ANC
As the Doha oil summit fails to reach a deal to freeze production, we examine the state of the global oil industry.
Business & Economy, Oil Price, Saudi Arabia
We explore how the work of medieval chemists has impacted the evolution of modern chemistry.
Science & Technology, Middle East, Islam


text:
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Wanna-be photographer from Berlin/Germany //D Plus I love to cosplay!! <3 Feel free to follow me Twitter: https://twitter.com/Free_zing__ Facebook: https://www.facebook.com/freezingphoto/ Instagram: freezing_cosplay
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ESTimating plant phylogeny: lessons from partitioning
While Expressed Sequence Tags (ESTs) have proven a viable and efficient way to sample genomes, particularly those for which whole-genome sequencing is impractical, phylogenetic analysis using ESTs remains difficult. Sequencing errors and orthology determination are the major problems when using ESTs as a source of characters for systematics. Here we develop methods to incorporate EST sequence information in a simultaneous analysis framework to address controversial phylogenetic questions regarding the relationships among the major groups of seed plants. We use an automated, phylogenetically derived approach to orthology determination called OrthologID generate a phylogeny based on 43 process partitions, many of which are derived from ESTs, and examine several measures of support to assess the utility of EST data for phylogenies.
A maximum parsimony (MP) analysis resulted in a single tree with relatively high support at all nodes in the tree despite rampant conflict among trees generated from the separate analysis of individual partitions. In a comparison of broader-scale groupings based on cellular compartment (ie: chloroplast, mitochondrial or nuclear) or function, only the nuclear partition tree (based largely on EST data) was found to be topologically identical to the tree based on the simultaneous analysis of all data. Despite topological conflict among the broader-scale groupings examined, only the tree based on morphological data showed statistically significant differences.
Based on the amount of character support contributed by EST data which make up a majority of the nuclear data set, and the lack of conflict of the nuclear data set with the simultaneous analysis tree, we conclude that the inclusion of EST data does provide a viable and efficient approach to address phylogenetic questions within a parsimony framework on a genomic scale, if problems of orthology determination and potential sequencing errors can be overcome. In addition, approaches that examine conflict and support in a simultaneous analysis framework allow for a more precise understanding of the evolutionary history of individual process partitions and may be a novel way to understand functional aspects of different kinds of cellular classes of gene products.
Higher order Spermatophyte phylogeny: an unresolved systematics problem
In this paper, we discuss the utility of incorporating EST data to address one of the more important plant phylogenetic questions concerning the hierarchical relationships of the several major seed plant lineages (angiosperms, Cycadales, Gingkoales, Gnetales and Coniferales). Phylogenetic relationships among seed plant groups have remained controversial, despite attempts to resolve Spermatophyte phylogeny using numerous character sources, both morphological
Figure 1
Conflicting Phylogenetic Hypotheses involving the seed plants
Conflicting Phylogenetic Hypotheses involving the seed plants. Morphological evidence (synapomophic characterstics shared between angiosperms and Gnetales) have shaped the anthophyte theory, where these two taxa form sister groups (Panel A; [1, 2, 5]). In contrast, most molecular studies postulate gymnosperms as a monophyletic group sister to all angiosperms, and place the Gnetales as a sister group to the conifers (Panels B and D; [7–9, 15, 16]). Adding to the controversy, a recent study involving phytochrome genes ([13]; Panel D) has placed the Gnetales as basal gymnosperms, with
In contrast, the majority of molecular phylogenies have postulated the gymnosperms to be a monophyletic group sister to all angiosperms. Most molecular studies place the Gnetales as a sister group to the conifers (Fig.
It is clear from the literature on seed plant phylogenetics that the addition of information relevant to the seed plants may be a viable way to solve this difficult problem. In addition, many studies on other taxa have demonstrated that the simultaneous analysis of multiple data partitions can result in an increase in overall branch support, despite conflict among the characters, due to emergent properties not evident in the separate analyses of individual data partitions
The (phylogenetic) trouble with ESTs
Genome level analyses have expanded our view of phylogenetics in many areas of the tree of life. With the production of whole genome DNA sequences of several taxa and large-scale EST databases as well as the incorporation of other genome enhanced technologies
Figure 2
Recent surge in gymnosperm sequence availability
Recent surge in gymnosperm sequence availability. Increase in number of available nucleotide sequences in the last five years. Contributions from EST sequencing projects at the New York Plant Genomics Consortium (NYPGC), involving
Sequencing errors and orthology determination pose challenges to the use of ESTs as a source of characters for systematics. There can be a high rate of sequencing error in raw EST data, since it is derived from single pass reads. A strategy to minimize this problem is contig assembly and EST clustering using several reads at every region (e.g.
Why partition? Hidden support and phylogenetic inference
Studies with large numbers of process partitions in a dataset exist
Implementing a different node support measure
Phylogenetic analyses and support
We constructed a matrix composed of 42 gene regions consisting of mitochondrial (6), chloroplast (16) and nuclear (20, including 19 ESTs) protein and DNA (18S rDNA) sequences. In addition, we included a morphological partition with 167 characters scored for the taxa in our study. The morphological matrix was developed for this study by coding morphological characters for
Additional File 1
Accession numbers for sequences used in the analysis. Accession numbers, by partition name, used to assemble the data matrix. Database of origin is indicated before each number. In some cases, more than one EST was used (i.e. clustered) to assemble a single partition.
Click here for file
Additional File 2
Table 2 – Data matrix used for analyses in this paper in NEXUS format. This file contains the raw data matrix of characters used in the phylogenetic analysis.
Click here for file
Figure 3
Simultaneous analysis (SA) tree of 43 data partitions
Simultaneous analysis (SA) tree of 43 data partitions. Single Most Parsimonious tree of the Spermatophyta, generated through the simultaneous analysis of 42 gene partitions, plus a morphological partition. Unboxed numbers on branches are bootstrap values. Node numbers are in white boxes. All other values are Partitioned Branch Support (PBS) values, as follows: Pink = total branch support (PBS); Yellow = apparent branch support (BS); Blue = hidden support (PHBS) with % total BS given below the PHBS. Branch support analysis for the cellular compartments is given to the right of the total support measures. Small boxes on right side of node indicate annotation of the total (on top) and hidden (on bottom) branch support for the three major cellular compartments. From left to right; Green = chloroplast; Blue = mitochondrion; Red = nuclear. Bootstrap values (2000 replicates) are shown on each node. The matrix has 15325 characters, 1085 phylogenetically (parsimony) informative. Tree Length: 6899 (exhaustive search); CI [90]: 0.918 (CI excluding uninformative characters: 0.739), RI [91]: 0.566.
In our analysis, cycads appear basal to a grouping of
Total branch support on the simultaneous analysis tree in Figure
Figure 4
Topological incongruence of individual process partitions with the SA tree
Topological incongruence of individual process partitions with the SA tree. The figure is a summary of bootstrap consensus trees (> 75% support) of individual (42 genes + morphology on red background) and broader (cell compartments on blue background; functional categories on yellow background) data partitions. Wide topological disagreement exists, as only one of the individual gene partitions (5, ribosomal protein S7) agrees with the simultaneous analysis (SA) tree. In broader partitions, only the nuclear data analysed together agrees with the SA tree at all nodes. Numbers in boxes indicate data partitions (see Additional File
Figure 5
Hidden support from individual data partitions
Hidden support from individual data partitions. This histogram shows the amount of total hidden branch support for the various process partitions examined in this study. Black bars indicate positive hidden support and fray bars indicate negative hidden support. A key to all of the partition abbreviations are given in
Effect of different partitioning strategies on hidden support
We also examined broader groupings of the data in order to explore the effect of different partitioning schema on apparent and hidden support (Figure
Additional File 3
Table 3 – Proportion of missing characters per taxon and data partition. This file lists the proportion of missing characters used in the analysis.
Click here for file
Figure 6
Amount of missing data for each taxon for each of the broader scale groupings of the data (chloroplast, mitochondria and nuclear)
Amount of missing data for each taxon for each of the broader scale groupings of the data (chloroplast, mitochondria and nuclear).
In general, the broad nuclear gene data partition proved to be the most consistent with the simultaneous analysis tree. Not only is the nuclear gene tree topologically identical to the simultaneous analysis tree, but all simultaneous analysis tree nodes also receive positive branch support, both hidden and apparent, from the nuclear data set. While this might be seen as an argument for the preferential use of nuclear genes (or ESTs) in phylogenetic analyses, over the molecular characters from other subcellular compartments (such as chloroplast and mitochondria), these subcellular compartments did contribute character support to the simultaneous analysis tree despite topological conflict. In addition, the topological differences among subcellular gene partitions examined using the ILD test (implemented in PAUP*
Exploration of incongruence among data partitions
In order to explore the interaction among data partitions analyzed separately, we calculated ILDs
Additional File 4
Table 4 – Pairwise analysis of congruence among individual partitions. Table showing significance scores when the Incongruence Length Difference (ILD) test was applied to pairwise comparisons among each individual partition. Statistically significant numbers (i.e. equal or smaller than 0.05), showing phylogenetic incongruence among partitions, are shaded.
Click here for file
Effect of missing taxa
Several of the partitions we used had missing taxa due, for example, to the lack of available sequence data for a given taxon for a particular gene region [see Additional Files
In our analyses, we established a matrix with six ingroup taxa and one outgroup, and no taxa were removed from the matrix at any time in our analysis regardless of amounts of missing data in the various partitions. This approach allowed us to explore the effect of the inclusion of taxa with missing data by examining branch support values contributed to the simultaneous analysis tree by partitions with varying amounts of missing data. In this case, we compared the contribution to support provided by those partitions that contained at least some data (but not necessarily the complete dataset) for all seven taxa to the group of partitions that were lacking data for one taxon for an entire partition (an individual gene region); to those that were lacking data for two taxa and so forth. In this way we were able to examine the affect of incompletely taxonomically sampled partitions.
The result of our analysis is shown in Figure
Figure 7
Effect of missing data on branch support
Effect of missing data on branch support. The randomness of EST sequencing results in some partitions not being found for all taxa. This histogram shows the effect of missing taxa in process partitions on support for each node. Support decreases as less taxa are included in the analysis however, these results do not take into account the varied amount and distribution of data available for each taxon (see text). The shading of the bars indicates the node in the SA tree: black = node 1; darker gray = node 2; lighter gray = node 3 and white = node 4. Branch support is given on the Y-axis, and the number of taxa in a process partition are given in clusters on the X-axis.
Effect of different functional classes of genes
We also partitioned the data set into classes of genes based on their cellular function. Our functional partitions were MnoPSR (Non-Photosynthetic or Respiratory Metabolism: 7 gene partitions), photosynthetic (11 gene partitions), respiration (7 gene partitions), signalling (3 gene partitions), structural (8 gene partitions), transcription factors (2 gene partitions) and genes of unknown function (4 gene partitions).
Figure 8
Node support from functional partitions
Node support from functional partitions. Support for each node in the simultaneous analysis tree from the various cellular function process partitions. The cellular function partitions are given in the legend to figure 4. The Y-axis indicates the branch support value. Node information is in clusters of process partitions as indicated on the X-axis. For simplicity, branch support values larger than ten are depicted here as 10.
While our results would suggest that conserved structural proteins and signalling proteins might be better at defining both deeper nodes and tree tip nodes, and proteins in the transcription factor and respiration class of genes might be best for nodes nearer the tips of the tree; the sample size of genes in functional partitions for this study is small. Nevertheless, these results do suggest a potential method for categorizing functional gene classes with respect to their congruence with a simultaneous or organismal phylogeny. As more ESTs are added to the sequence database, the sample sizes of these functional classes will become larger and a more rigorous test of the role of functional class in phylogenetic analysis may be possible.
Where to from here?
The approach we describe here, where support for the SA tree is estimated for each process partition, will also pinpoint those partitions that disagree or conflict with the overall general pattern of divergence of the taxa in the analysis. If one assumes that the SA tree best represents evolutionary history of the taxa involved, then such partitions are in conflict with overall organismal history of the taxa in the analysis. This approach then would provide a method for detecting process partitions that might be selected for or have experienced drift and such partitions might be important in some of the more interesting organismal differences amongst the taxa in the analysis.
One final and important aspect of the present analysis highlights a problem that will be prevalent in future genomic level phylogenetic studies. This problem concerns the almost continual revision of the overall phylogenetic hypothesis for a set of taxa. For instance, as more and more EST data are added to the database, more and more process partitions can be added to an analysis. This will effectively create a growing matrix that might even expand daily. With the addition of each new process partition to an analysis, all support values and other tree metrics such as bootstrap values
• Simultaneous analysis using 42 gene partitions and a morphological partition yield a phylogenetic hypothesis with a monophyletic gymnosperms which is at odds with the Anthophyte hypothesis.
• Addition of short EST sequences to a data set can enhance a phylogenetic analysis, if the problems of sequence quality and orthology are overcome.
• The majority of support in this study is hidden support, meaning that the support is not immediately apparent in single gene partitions.
• Completeness of data partitions with respect to full complement of taxa had a large affect on levels of support in phylogenetic analysis. In our study example with seven taxa, support from partitions that had sequences for five or fewer taxa was nonexistent. However, variation in the amount and distribution of data within partitions may also play a role.
• When phylogenetic incongruence between a partitioned functional class of genes (such as transcription factors) and the organismal phylogeny is detected, this result suggests that the partition has experienced a unique evolutionary history relative to the organisms. This different evolutionary history can be used as a signpost of altered evolutionary pressure in a particular class of genes. In this way, incongruence of a particular class of genes (such as transcription factors) in a partitioned analysis allow us to establish hypotheses about the evolution and potential function of these gene classes.
Orthology determination and phylogentic analyses
Many studies use pairwise sequence comparison schemes, such as BLAST
Since we are ultimately interested in exploring the characters associated with particular evolutionary novelties, we use a character-based alternative to distance based methods for the identification of orthologous gene regions. The tree-building approach to orthology determination involves the generation of gene family trees in order to identify the orthologous gene family member for each EST sequence. Within a character-based parsimony framework, nodes are defined by shared derived characters.
Without automation, this approach would be prohibitively time consuming and labor intensive and would greatly restrict the use of genomic-scale EST data in parsimony based analyses since the placement of ESTs into orthology groups using this character-based approach would require manual rebuilding of gene family trees for each new EST to be classified. Therefore, during the course of this study, we developed automated methods for orthology determination within a parsimony framework: OrthologID
We use only completely sequenced genomes for constructing gene family guide trees with OrthologID in order to minimize the possibility of the erroneous placement of query sequences due to missing data. If gene family guide trees had been constructed using partially sequenced genomes, it is possible that some gene family members would be missing, in which case it could be possible that queries orthologous to these missing gene family members would be incorrectly placed. The current database of plant genomes will soon be expanded to include complete genomes from other phylogenetic lineages, including prokaryotes and non-plant eukaryotes.
OrthologID automatically searches the local database of completely sequenced plant genomes and performs an initial clustering of gene sequences into putative gene families, using NCBI BLAST
Once EST orthologs had been identified, we manually assembled a process partition matrix for each orthologous gene region for all of the seven chosen plant taxa; sequences for the moss
Authors' contributions
MGE and JEBdlT were responsible for the primary collection of information from the EST databases and for the construction of the phylogenetic matrix. EDB and JEBdlT generated many of the EST sequences for
Additional File 5
Table 5 – Key to partition names.
Click here for file
The authors acknowledge Rob Martienssen and W. Richard McCombie (CSHL), Indra Neil Sarkar (AMNH) and other members of the New York Plant Genomics Consortium (NYPGC) for stimulating discussion and comments on the manuscript. The work in this manuscript was supported by an NSF Plant Genome grant #DBI -0421604 to GMC, DWS, and RD. MGE, RD and DWS thank the continued support of the Lewis B and Dorothy Cullman Program in Molecular Systematics at the AMNH and the NYBG.
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Man-Portable Communication Systems Market to Grow at a CAGR of 5.56% to 2019
(M2 PressWIRE Via Acquire Media NewsEdge) RnRMarketResearch.com adds "Man-Portable Communication Systems Market by Platforms (Land, Airborne, Naval), by Application (Software Defined Radios, SATCOM, Encryption, Smartphones, Homeland Security, Commercial), & by Geography [North America, Asia-Pacific, Europe, the Middle East & ROW) -Forecast & Analysis (2014-2019) " report to its research database.
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- categoryCasual Pantstagged by SYSTEM, 7/20/11
- topicClothingtagged by SYSTEM, 7/20/11
- categoryDenimtagged by SYSTEM, 7/20/11
- categoryJeanstagged by SYSTEM, 7/20/11
- categoryPants Shortstagged by SYSTEM, 7/20/11
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I have a jean...(The Ultimate Jean Thread For Beginners) - ask questions here. - Page 1254post #18796 of 2136211/29/12 at 8:46pmpost #18797 of 2136211/29/12 at 8:51pm
post #18798 of 2136211/30/12 at 12:18am
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post #18799 of 2136211/30/12 at 9:38am
- Posts: 277
- Joined: 11/2012
- Select All Posts By This User
Ok.. i've lurked for a long time now but im finally going to ask for some help in this thread..
- Posts: 27
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I'm looking for advice on my first pair of nice jeans that will fit similar to a Levi 514 cut. I've never owned jeans that cost more than $50 and recently have only been wearing 514's in a size 31x32 (in dress slacks i'm more like 30x30, but i got them a little long for stacking and a tiny bit loose in the waist for room in the "box" area).
I'd like to keep it around $150 or under, but i'm always open to good deals and ultimately just want the most bang for my buck. Looking for something versatile in a not-too-dark indigo (unless they're black... dont own any black jeans).
If it matters i'm 5'8, 160 athletic build.
Thanks for any advice -- much appreciated.post #18800 of 2136211/30/12 at 11:44am
- notwithit
- Pullup laureate
^ I'm partial to the Uniqlo regular fit made-in-Japan model as far as 514 equivalents are concerned: http://www.uniqlo.com/us/store/made-for-all/men-made-in-japan-regular-fit-jeans/073554-68
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They don't have the indigo ones in stock right now, though.
My noob question: Where are all the cool kids getting their jeans repaired these days? My Ande Whall grifters need some work, and Self Edge doesn't take mail orders anymore.post #18801 of 2136211/30/12 at 1:07pm
post #18802 of 2136211/30/12 at 2:21pmQuote:
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What about the denim should I be looking for? Thanks. And yeah, they were 30% off. Still over priced, but made in the USA and they fit well.post #18803 of 2136211/30/12 at 2:33pmQuote:
glad they fit and as long as you got them on sale but sanforized denim has been treated so as not to shrink very much where unsanforized denim can shrink significantly. IIRC there isn't a surefire way to tell just by looking, but individual brands can tell you which make/model and what denim is used for that style. Since many people here are wearing/breaking-in raw denim, they most likely have not washed the jeans yet until the fading and creasing has reached a point that they like. Check the sales thread... so much on sale you're bound to find something.
I drove by this place the other day... they are just repair specialists?post #18804 of 2136211/30/12 at 2:38pm
post #18805 of 2136211/30/12 at 4:58pmQuote:Originally Posted by Kid Nickels
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glad they fit and as long as you got them on sale but sanforized denim has been treated so as not to shrink very much where unsanforized denim can shrink significantly. IIRC there isn't a surefire way to tell just by looking, but individual brands can tell you which make/model and what denim is used for that style. Since many people here are wearing/breaking-in raw denim, they most likely have not washed the jeans yet until the fading and creasing has reached a point that they like. Check the sales thread... so much on sale you're bound to find something.
I drove by this place the other day... they are just repair specialists?
Thanks, but I think you misunderstood. I already emailed the company, and the rep seemed kinda clueless. I understand a lot of labels actually do give certain info, whether about shrinkage or actually as to whether our not it is sanforized. I wasn't sure what the minimal amount of info I would find would be on the tag, or if that kinda thing is standard.post #18806 of 2136211/30/12 at 5:08pm
post #18807 of 2136211/30/12 at 5:13pmpost #18808 of 2136211/30/12 at 8:23pmI more or less knew what i was getting into with the 7s. they're only 11 oz, but i liked some of the stuff about them.
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But yeah, if they're unsanforized, it'll be really weird, because like I said...the only unsanforized stuff they've probably ever made, except maybe a jacket or something. I honestly do not think the sales rep knew what sanforization was, looked it up, saw it was a method of preshrinking, and, since they are raw and not shrunk from washing, assumed telling me they were not preshrunk was good enough. Which is pretty annoying, really.post #18809 of 2136212/4/12 at 11:58am
post #18810 of 2136212/4/12 at 1:44pm
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Goal: I'm looking for some well-fitting entry level selvedge - one mid-weight indigo and one light-weight gray/black - but I feel like a top-heavy freak trying on Uniqlo's MIJ, even in Regular Fit. Please help me find the perfect pair! If entry level is out of the question, I'll pay whatever it takes to make this work, but it's my first real pair of denim so I'd prefer to screw up with a less expensive pair. Please note that I live in NYC, so I can go window shopping if you think that's a better choice.
Fit: My best fitting pair is no longer made, and I'm not sure exactly what it is. From the label, I know these things: 100% cotton, "chuckin slim" converse by varvatos. It's very lightweight and feels a bit stretchy. My only complaint is that the rise is a tad short and I'd like the opening to be smaller.
Here are the measurements of the best fitting pair with some notes on fit:
Label Waist = 31.0" (also what I wear in Levi's true-to-size)
True Waist = 34.5" (hard to measure due to age / stretchiness)
Front rise = 10.5" but should go up to 10.75"
Thighs = 22.0" but they stretch.
Actual Body's Thighs = 24.25" with jeans on, 23.25" with jeans off
Knee 8.25"
Opening = 15.0" but want to come down to 14.5"
Inseam 32.5"
Style: I live in Williamsburg, Brooklyn and I'd self-describe as a Yupster. Denim is just one of those things I never quite got just right. I'd say the truly "skinny jean" look is a little beyond me, but slim and slim straight, or Levi 508 & 520-stretch work just fine in my style. The only real killer is that having my pants skin-tight against my thighs makes me look ridiculous.
Thanks for any guidance you can provide rawdenim pros!
Edited by bdud - 12/4/12 at 2:11pmReturn HomeBack to Forum: Streetwear and Denim
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KCLSU has become the first educational institution to openly move their money to ethical banks
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Kazakh President Regards Russia as a Major Partner
January 13, 2005
Kazakh President Regards Russia as a Major Partner RIA Novosti reported, President Nursultan Nazarbayev said at a meeting with his Russian counterpart, Vladimir Putin, that Russia was Kazakhstan's major partner on the international arena. Mr Nazarbayev said bilateral commodity turnover had risen 47% over 2004 and reached $7 billion. Russia accounts for 25% of Kazakhstan's foreign trade, according to the president. Mr Nazarbayev said the current border crossing arrangements would be preserved for both countries' residents after the borders had been delimited. "There are talks around the problem. However, we are discussing delimitation, not demarcation. There will be no broken borders, and the [current] border crossing regulations for Russians and Kazakhs will be preserved," said Mr Nazarbayev. 12 January 2005
Source: RIA Novosti
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text:
In this high speed video captured by a Phantom Flex, author Neal Stephenson slices up fruit and a water bottle with an Angus Trim longsword for a ChefSteps sous-vide course.
Thanks Benjy!
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