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Publications (49)166.47 Total impact
Article: Bionano Interactions of MCF-7 Breast Tumor Cells with a Transferrin Receptor Targeted Nanoparticle[Show abstract] [Hide abstract]
ABSTRACT: Although transferrin receptor (TfR) is widely accepted as a target for cancer therapy, few studies have elaborated on delivery efficiency of TfR upon interactions with TfR-targeted nanomedicine. Here, a micellar system employing TfR-specific 7peptide (Histidine-Alanine-Isoleucine-Tyrosine- Proline-Arginine-Histidine, HAIYPRH, 7pep) as the targeting moiety was constructed; and its endocytosis, intracellular trafficking as well as influence on TfR expression and in vivo tumor targeting were explored in the MCF-7 tumor model. In contrast to unmodified micelles, 7pep modification enhanced the cellular uptake of micelles without altering endocytic pathways, and slowed down the trafficking of micelles to lysosomes without changing the final intracellular co-localization. Interestingly, cellular TfR level was increased by 7pep-modified micelles. Furthermore, receptor saturation and recovery was observed in vivo. In conclusion, this study comprehensively investigated the bio-nano interaction between TfR positive tumors and 7pep-modified micelles, and provided scientific information for cancer therapy with receptor-mediated nanomedicines.
Article: Synergistic inhibition of breast cancer by co-delivery of VEGF siRNA and paclitaxel via vapreotide-modified core-shell nanoparticles[Show abstract] [Hide abstract]
ABSTRACT: A somatostatin analog, vapreotide (VAP), can be used as a ligand for targeting drug delivery based on its high affinity to somatostatin receptors (SSTRs), which is overexpressed in many tumor cells. RNA interference plays an important role on downregulation of vascular endothelial growth factor (VEGF), which is important for tumor growth, progression and metastasis. To improve tumor therapy efficacy, the vapreotide-modified core-shell type nanoparticles co-encapsulating VEGF targeted siRNA (siVEGF) and paclitaxel (PTX), termed as VAP-PLPC/siRNA NPs, were developed in this study. When targeted via somatostatin receptors to tumor cells, the VAP-PLPC/siRNA NPs could simultaneously delivery siVEGF and PTX into cells and achieve a synergistic inhibition of tumor growth. Interestingly, in vitro cell uptake and gene silencing experiments demonstrated that the targeted VAP-PLPC/siRNA NPs exhibited significant higher intracellular siRNA accumulation and VEGF downregulation in human breast cancer MCF-7 cells, compared to those of the non-targeted PEG-PLPC/siRNA NPs. More importantly, in vivo results further demonstrated that the targeted VAP-PLPC/siRNA NPs had significant stronger drug distribution in tumor tissues and tumor growth inhibition efficacy via receptor-mediated targeting delivery, accompany with an obvious inhibition of neovascularization induced by siVEGF silencing. These results suggested that the co-delivery of siRNA and paclitaxel via vapreotide-modified core-shell nanoparticles would be a promising approach for tumor targeted therapy.
Article: Inhibition of Metastatic Tumor Growth and Metastasis via Targeting Metastatic Breast Cancer by Chlorotoxin-Modified Liposomes[Show abstract] [Hide abstract]
ABSTRACT: A liposome system modified with chlorotoxin (ClTx), a scorpion venom peptide previously utilized for targeting brain tumors, was established. Its targeting efficiency and anti-metastasis behavior against metastatic breast cancer highly expressed MMP-2, the receptor of ClTx, were investigated. 4T1, a metastatic breast cancer cell line derived from a murine breast tumor, was selected as the cell model. As the results, the ClTx-modified liposomes displayed specific binding to 4T1 as determined by flow cytometry and confocal imaging. The cytotoxicity assay revealed that the ClTx modification increased the toxicity compared with non-modified liposomes. In addition, the modified liposomes also exhibited high in vivo targeting efficiency effect in the BALB/c mice bearing 4T1 tumors. Importantly, this system inhibited the growth of metastatic tumor and prevented the incidence of lung metastasis in mice bearing 4T1 tumors with only low systemic toxicity. The data obtained from the in vitro and in vivo studies confirmed that the ClTx-modified liposomes increased the drug delivery to metastatic breast cancers. This study proved that the ClTx-modified liposomes had the targeting ability to metastatic breast cancer in addition to brain cancer, and displayed obvious anti-metastasis effect. Generally, it may provide a promising strategy for metastatic breast cancer therapy.
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ABSTRACT: Our previous study had reported that cholesterol-grafted poly(amidoamine) (rPAA-Chol polymer) was able to self-assemble into cationic nanoparticles and act as a potential carrier for siRNA transfection. In this study, the core-shell type lipid/rPAA-Chol hybrid nanoparticles (PEG-LP/siRNA NPs and T7-LP/siRNA NPs) were developed for improving in vivo siRNA delivery by modifying the surface of rPAA-Chol/siRNA nanoplex core with a lipid shell, followed by post-insertion of polyethylene glycol phospholipid (DSPE-PEG) and/or peptide (HAIYPRH, named as T7) modified DSPE-PEG-T7. The integrative hybrid nanostructures of LP/siRNA NPs were evidenced by dynamic light scattering (DLS), confocal laser scanning microscope (CLSM), cryo-transmission electron microscope (Cryo-TEM) and surface plasmon resonance (SPR) assay. It was demonstrated that the T7 peptide modified LP/siRNA NPs (T7-LP/siRNA NPs) exhibited uniform and spherical structures with particle size of 99.39 ± 0.65 nm and surface potential of 42.53 ± 1.03 mV, and showed high cellular uptake efficiency and rapid endosomal/lysosomal escape ability in MCF-7 cells. Importantly, in vitro gene silencing experiment demonstrated that both of pegylated and targeted LP/siEGFR NPs exhibited significantly stronger downregulation of EGFR protein expression level in MCF-7 cells, compared to that of the physical mixture of siRNA lipoplexes and rPAA-Chol/siRNA nanoplexes. In vivo tumor therapy on nude mice bearing MCF-7 tumors further confirmed that the targeted T7-LP/siEGFR NPs exhibited the greatest inhibition on tumor growth via transferrin receptor-mediated targeting delivery, without any activation of immune responses and significant body weight loss following systemic administration. These findings indicated that the core-shell type T7-LP/siRNA nanoparticles would be promising siRNA delivery systems for in vivo tumor-targeted therapy.
Article: Synthesis and Biological Evaluation of Novel Folic Acid Receptor-Targeted, β-Cyclodextrin-Based Drug Complexes for Cancer Treatment[Show abstract] [Hide abstract]
ABSTRACT: Drug targeting is an active area of research and nano-scaled drug delivery systems hold tremendous potential for the treatment of neoplasms. In this study, a novel cyclodextrin (CD)-based nanoparticle drug delivery system has been assembled and characterized for the therapy of folate receptor-positive [FR(+)] cancer. Water-soluble folic acid (FA)-conjugated CD carriers (FACDs) were successfully synthesized and their structures were confirmed by 1D/2D nuclear magnetic resonance (NMR), matrix-assisted laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS), high performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR), and circular dichroism. Drug complexes of adamatane (Ada) and cytotoxic doxorubicin (Dox) with FACD were readily obtained by mixed solvent precipitation. The average size of FACD-Ada-Dox was 1.5-2.5 nm. The host-guest association constant K a was 1,639 M(-1) as determined by induced circular dichroism and the hydrophilicity of the FACDs was greatly enhanced compared to unmodified CD. Cellular uptake and FR binding competitive experiments demonstrated an efficient and preferentially targeted delivery of Dox into FR-positive tumor cells and a sustained drug release profile was seen in vitro. The delivery of Dox into FR(+) cancer cells via endocytosis was observed by confocal microscopy and drug uptake of the targeted nanoparticles was 8-fold greater than that of non-targeted drug complexes. Our docking results suggest that FA, FACD and FACD-Ada-Dox could bind human hedgehog interacting protein that contains a FR domain. Mouse cardiomyocytes as well as fibroblast treated with FACD-Ada-Dox had significantly lower levels of reactive oxygen species, with increased content of glutathione and glutathione peroxidase activity, indicating a reduced potential for Dox-induced cardiotoxicity. These results indicate that the targeted drug complex possesses high drug association and sustained drug release properties with good biocompatibility and physiological stability. The novel FA-conjugated β-CD based drug complex might be promising as an anti-tumor treatment for FR(+) cancer.
Article: Self-assembly cationic nanoparticles based on cholesterol-grafted bioreducible poly(amidoamine) for siRNA delivery[Show abstract] [Hide abstract]
ABSTRACT: In this study, a series of bioreducible poly(amidoamine)s grafting different percentages of cholesterol (rPAA-Ch14: 14%, rPAA-Ch29: 29%, rPAA-Ch57: 57% and rPAA-Ch87: 87%) was synthesized and used for siRNA delivery. These amphiphilic polymers were able to self-assemble into cationic nanoparticles in aqueous solution at low concentrations. The nanoparticle formation was evidenced via cryo-transmission electron microscope (Cryo-TEM) and dynamic light scattering analysis. The average hydrodynamic size of rPAA-Ch blank nanoparticles was about 80-160 nm with zeta potential of 50-60 mV. Also, the effects of different percentages of cholesterol grafted onto rPAA on physicochemical characteristics, in vitro cytotoxicity, cellular uptake, VEGF gene silencing efficacy and translocation mechanism of rPAA-Ch/siRNA complexes were investigated. The results showed that rPAA-Ch57 polymer was not only able to form stable nanocomplexes and possess high cell uptake, but also to exhibit the best in vitro VEGF gene silencing efficacy and the best in vivo tumor growth inhibition effect when it was formulated with VEGF-siRNA. Moreover, the observations of confocal laser scanning microscope (CLSM) and the study of cholesterol competitive inhibition demonstrated that endosomal/lysosomal escape and cytoplasmic dissociation of rPAA-Ch57/siRNA complexes were dependent on the "proton sponge effect" and disulfide cleavage, following internalization with cholesterol-related endocytosis pathway and subsequent transportion into endosomes/lysosomes. These findings indicated that the rPAA-Ch57 polymer should be a promising and potent carrier for siRNA delivery.
Article: A comparative study of three ternary complexes prepared in different mixing orders of siRNA/redox-responsive hyperbranched poly (amido amine)/hyaluronic acid[Show abstract] [Hide abstract]
ABSTRACT: In this study, a novel redox-responsive hyperbranched poly(amido amine) (named PCD) was synthesized and used as a cationic polymer to form a ternary complex with small interfering RNA (siRNA) and hyaluronic acid (HA) for siRNA delivery. Here, it is hypothesized that different mixing orders result in different assembly structures, which may affect the siRNA delivery efficiency. To investigate the effects of mixing orders on siRNA delivery efficiency in two human breast cancer cell lines, three ternary complexes with different mixing orders of siRNA/PCD/HA were prepared and characterized: mixing order I (initially prepared siRNA/PCD binary complex further coated by negatively charged HA), mixing order II ( initially prepared HA/PCD binary complex further incubated with siRNA), and mixing order III ( initially prepared siRNA/HA mixture further electrostatically compacted by positively charged PCD). With an optimized siRNA/PCD/HA charge ratio of 1/20/16, the particle sizes and zeta potentials of these ternary complexes were 124.8 nm and 27.3 mV (mixing order I), 147.5 nm and 29.9 mV (mixing order II), and 128.8 nm and 19.4 mV (mixing order III). Also, the effects on stability, cellular uptake, and gene silencing efficiency of siRNA formulated in ternary complexes with different mixing orders were investigated. The results showed that mixing orders I and III displayed better siRNA transfection and protection than mixing order II in human breast cancer MCF-7 and MDA-MB-231 cells. More interesting, at the siRNA/PCD/HA charge ratio of 1/20/16, the gene silencing effects on vascular endothelial growth factor expression in MDA-MB- 231 cells were as follows: mixing order III > mixing order I > mixing order II. Based on these results, a likely explanation for the difference in functionality dependent on mixing orders is the formation of different assembly structures. These results may help future optimization of siRNA ternary complexes for achieving better delivery efficiencies, especially for target-specific siRNA delivery to cells with HA receptor overexpression.
Article: Enhanced anticancer activity of gemcitabine coupling with conjugated linoleic acid against human breast cancer in vitro and in vivo[Show abstract] [Hide abstract]
ABSTRACT: Gemcitabine (GEM) is a nucleoside analog agent against a wide variety of tumors. To overcome its limitation of rapid metabolism in vivo that results in short circulation time and poor antitumor efficacy, a novel prodrug (CLA-GEM conjugate) has been developed through the covalent coupling of conjugated linoleic acid (CLA) to N(4)-amino group of GEM. The chemical structure of CLA-GEM conjugate was identified by NMR, FTIR and other methods. From in vitro tests, it was demonstrated that the linkage with CLA increased the plasma stability of GEM as well as the antitumor activity against human breast tumor cells (MCF-7). Importantly, it also altered the transport pattern of GEM across cell membrane (MCF-7 and MDA-MB-231), evidenced by the little effect of nucleoside transporter inhibitors (NBMPR and dipyridamole) on the IC(50) values of CLA-GEM, instead of the great effect on that of unmodified GEM. In vivo pharmacokinetic study showed that the CLA-GEM conjugate had a longer plasma half-life and a higher bioavailability compared to that of unmodified GEM. Significant stronger antitumor activity was observed in the nude mice xenografted MCF-7 breast tumor after treated with CLA-GEM than that of unmodified GEM, while no significant body weight loss was found in all treatments. In conclusion, the novel CLA-GEM conjugate prepared in this study would be a promising prodrug of gemcitabine for future clinical use.
Article: Self-assembly nanomicelles based on cationic mPEG-PLA-b-Polyarginine(R-15) triblock copolymer for siRNA delivery[Show abstract] [Hide abstract]
ABSTRACT: Due to the absence of safe and effective carriers for in vivo delivery, the applications of small interference RNA (siRNA) in clinic for therapeutic purposes have been limited. In this study, a biodegradable amphiphilic tri-block copolymer (mPEG(2000)-PLA(3000)-b-R(15)) composed of monomethoxy poly(ethylene glycol), poly(d,l-lactide) and polyarginine was synthesized and further self-assembled to cationic polymeric nanomicelles for in vivo siRNA delivery, with an average diameter of 54.30 ± 3.48 nm and a zeta potential of approximately 34.8 ± 1.77 mV. The chemical structures of the copolymers were well characterized by (1)H NMR spectroscopy and FT-IR spectra. In vitro cytotoxicity and hemolysis assays demonstrated that the polymeric nanomicelles showed greater cell viability and haemocompatibility than those of polyethyleneimine (PEI) or R(15) peptide. In vitro experiments demonstrated that EGFR targeted siRNA formulated in micelleplexes exhibited approximately 65% inhibition of EGFR expression on MCF-7 cells in a sequence-specific manner, which was comparable to Lipofectamine™ 2000. The results of intravenous administration showed Micelleplex/EGFR-siRNA significantly inhibited tumor growth in nude mice xenografted MCF-7 tumors, with a remarkable inhibition of EGFR expression. Furthermore, no positive activation of the innate immune responses and no significant body weight loss was observed during treatment suggested that this polymeric micelle delivery system is non-toxic. In conclusion, the present nanomicelles based on cationic mPEG(2000)-PLA(3000)-b-R(15) copolymer would be a safe and efficient nanocarrier for in vivo delivery of therapeutic siRNA.
Article: Comprehensive studies on the interactions between chitosan nanoparticles and some live cells[Show abstract] [Hide abstract]
ABSTRACT: As more and more oral formulations of nanoparticles are used in clinical contexts, a comprehensive study on the mechanisms of interaction between polymer nanoparticles and live cells seems merited. Such a study was conducted and the results were compared to the polymer itself in order to demonstrate different kinds of effects that are brought into the cell by polymer and its nanoparticles, especially the effects on the biomembrane. Several techniques, including surface plasmon resonance (SPR), Fourier transformed infrared spectroscopy (FTIR), Raman spectroscopy, fluorescence polarization spectroscopy (FP), flow cytometry (FCM) with quantitative analysis, and confocal images with antibody staining were employed toward this end. The cytotoxicity in vitro was also evaluated. Chitosan (CS), a polycationic polymer, was used to prepare the nanoparticles. We demonstrate that chitosan nanoparticles (CS-NP) induce strong alterations in the distribution of membrane proteins, fluidity of membrane lipids, and general membrane structure. Furthermore, the uptake of CS-NP into Caco-2 cells was found to have a similar mechanism to that of CS molecules, but the differences in degree were noted. These results indicate that positive charge and nanoscale size were the factors that most significantly affected the interactions between the nanoparticles of polycationic polymers and live cells. However, no difference in cytotoxicity toward the Caco-2 cells was found between CS and CS-NP. This supports the idea that CS-NP is an effective and safe carrier for oral drug delivery. KeywordsNanoparticles–Polycationic polymers–Thymopentin–Polymer interactions–Caco-2 Cells–C6 Cells–Nanomedicine
Article: LC Determination of Icariside II in Rat Plasma and Tissues: Application to a Tissue Distribution Study[Show abstract] [Hide abstract]
ABSTRACT: A sensitive and reliable reversed-phase liquid chromatography (RP-LC) with ultraviolet (UV) detection has been developed and validated for the quantification of Icariside II in rat plasma and tissues using Fermononetin as the internal standard. Protein precipitation and liquid–liquid extraction were utilized for plasma and tissue sample preparation, respectively. The analysis was successfully carried out on an Agilent SB-C18 column (5 μm, 4.6 × 250 mm) with the implementation of the following conditions: a mobile phase of phosphoric acid solution (0.1%, v/w)–Acetonitrile (55:45, v/v), a flow rate of 1 mL min−1, a column temperature of 25 °C and a detection wavelength of 270 nm. Good linear relationships of calibration curves were obtained (r 2 > 0.9906) over the investigated concentration range with plasma and tissue samples. The lower limit of quantification (LLOQ) and the limit of detection (LOD) were 0.1 and 0.02 μg g−1, respectively (for plasma sample, they were 0.05 and 0.1 μg mL−1, respectively). The developed method which was embodied with good precision, accuracy, recovery and stability was corroborated to satisfy the requirements for biomedical sample analysis. This method has been successfully applied to tissue distribution study of Icariside II in rats after a single intravenous dose at 12.5 mg kg−1. Results suggested that Icariside II was distributed to rat tissues rapidly with greater initial concentrations in kidney, lung and liver. Moderate initial distributions were obtained in rat muscle, heart, bone, spleen and plasma. Low amount of Icariside II was detected in testes, and no Icariside II could be detected in the brain.
Article: Preparation of the albumin nanoparticle system loaded with both paclitaxel and sorafenib and its evaluation in vitro and in vivo[Show abstract] [Hide abstract]
ABSTRACT: Paclitaxel and sorafenib loaded albumin nanoparticles (PTX-SRF-BSA-NPs) were prepared and studied here to avoid the toxicities from the excipients in the Taxol® and explore the effect of such combination on the antitumour efficacy and toxicity. PTX-BSA-NPs and so on were used as controls. The particle size, zeta potential, encapsulation efficiency and morphology were evaluated. Less than 70% of each drug released within 24 h. PTX and SRF existed as molecular or amorphous form in the PTX-SRF-BSA-NPs. The particle size did not change much after 2-month storage in freeze-dried form or 24 h in suspension. The treatment with PTX-SRF-BSA-NPs (7.5 mg kg(-1) PTX + 7.5 mg kg(-1) SRF) exhibited lower myelosuppression than PTX-BSA-NPs (15 mg kg(-1) PTX) while it remained or increased the antitumour effect in mice tumour models. Compared with the solution containing the same level of PTX and SRF, PTX-SRF-BSA-NPs demonstrated significantly lower haemolysis and myelosuppression effect.
Article: A Lipid Nanoparticle System Improves siRNA Efficacy in RPE Cells and a Laser-Induced Murine CNV Model[Show abstract] [Hide abstract]
ABSTRACT: To explore the possibility of the PEGylated liposome-protamine-hyaluronic acid nanoparticles (PEG-LPH-NP) loaded with siRNA (PEG-LPH-NP-S) in ARPE19 cells and a laser-induced rat model for the treatment of choroidal neovascularization (CNV). PEG-LPH-NP-S was characterized by dynamic light scattering and transmission electron microscopy (TEM). The encapsulation efficiency of siRNA in PEG-LPH-NP was analyzed by ultracentrifugation, whereas the protection of siRNA by PEG-LPH-NP was evaluated by electrophoresis. Human RPE cells (ARPE19) were used as the cell model for the studies of cellular uptake and the inhibition of VEGFR1 expression, visualized by a laser scanning confocal microscope. The area of CNV in the laser-induced rat model after intravitreous injection was measured. The distribution of the lipid nanoparticles in the retina after intravitreous administration was investigated by fluorescence microscopy. Finally, the TUNEL test and morphologic observation of the retina were conducted. It was indicated that PEG-LPH-NP-S was approximately 132 nm in particle size with a positive charge of approximately 20 mV, whereas the encapsulation efficiency of siRNA in PEG-LPH-NP was >95%. PEG-LPH-NP could protect the siRNA load and could facilitate the intracellular delivery of fluorescein-labeled siRNA to ARPE19 cells. VEGFR1 expression in ARPE19 cells could be inhibited, and the CNV area in the murine model could be reduced more effectively by PEG-LPH-NP-S compared with naked siRNA and by PEG-LPH-NP with negative siRNA. It seems that the toxicity of PEG-LPH-NP-S on the rat retina is low, based on the results of TUNEL testing and morphologic observation. PEG-LPH-NP may be a promising lipid nanoparticle system for the siRNA treatment of CNV.
Article: Sequential treatment of drug-resistant tumors with RGD-modified liposomes containing siRNA or doxorubicin[Show abstract] [Hide abstract]
ABSTRACT: Tumor targeting drug delivery systems are being the ideal carriers of systemic administration for tumor therapy. We have reported previously that RGD peptide (arginine-glycine-aspartic acid)-modified liposomes containing drugs could increase targeting to tumor by binding with the integrin receptors overexpressed on tumor cells. RNA interference plays an important role on down-regulation of P-glycoprotein (P-gp), which is a drug efflux transporter overexpressed on multi-drug-resistant (MDR) tumor cells. To improve MDR tumor therapy, sequential treatment strategy with RGD-modified liposomes containing P-gp targeted small interference (siRNA) or doxorubicin (DOX) was reported in this study. When targeted via RGD to tumor-cell-surface and tumor neovasculature endothelial cell receptors, cationic liposomes could specifically deliver siRNAs to tumor cells and thus reverse drug resistance by down-regulation of P-gp, following administration of targeted liposomes containing DOX that inhibit formerly drug-resistant tumors. From the current results, the combination use of DOX and P-gp targeted siRNA showed significantly higher in vitro cytotoxicity in tumor cells than liposomal DOX alone. In vivo studies in a mouse model of drug-resistant MCF7/A tumor demonstrated significantly greater inhibition of tumor growth followed by the sequential treatment of RGD-modified liposomes containing siRNA or DOX when compared to liposomal DOX alone. Also, ex vivo tissue imaging studies have shown the accumulation of siRNA and DOX in tumors at same site-specific manner. These results suggested that the sequential treatment of P-gp gene silencing and cytotoxic drug with RGD-modified liposome drug delivery system could be a promising clinical treatment for drug-resistant tumors.
Article: Targeted delivery of RGD-modified liposomes encapsulating both combretastatin A-4 and doxorubicin for tumor therapy: In vitro and in vivo studies[Show abstract] [Hide abstract]
ABSTRACT: Arg-Gly-Asp (RGD) modified doxorubicin-loaded liposomes could improve anticancer effect, and vascular disrupting agents (VDAs) could induce a rapid and selective shutdown of the blood vessels of tumors. We propose that RGD-modified liposomes for co-encapsulation and sequential release of vascular disrupting agent combretastatin A-4 (CA-4) and cytotoxic agent doxorubicin (Dox) could enhance tumor inhibition responses. In this study, we encapsulated Dox and CA-4 in RGD-modified liposomes. The release rate of Dox was proved to be much slower than that of CA-4 in vitro. Flow cytometry and laser confocal scanning microscopy clearly showed that RGD-modification promoted intracellular uptake of liposomal drugs by B16/B16F10 melanoma tumor cells and human umbilical vein endothelial cells (HUVECs). Cytotoxicity assay showed that the IC(50) of RGD-modified liposomes was lower than that of the corresponding unmodified liposomes. Therapeutic benefits were examined on B16F10 melanoma tumors subcutaneously growing in C57BL/6 mice. In vivo study demonstrated that RGD-modified liposomes exhibited the most pronounced tumor regression effect when both CA-4 and Dox were co-encapsulated. These results suggest that the targeted drug delivery system for co-encapsulation of vascular disrupting agents and anticancer agents may be a promising strategy for cancer treatment.
Article: Preparation and evaluation of docetaxel-loaded albumin nanoparticles for intravenous administration
Article: Effects of pH-sensitive nanoparticles prepared with different polymers on the distribution, adhesion and transition of Rhodamine 6G in the gut of rats[Show abstract] [Hide abstract]
ABSTRACT: To investigate the effect of different enteric polymers on the characteristics of pH-sensitive nanoparticles, Rhodamine 6G (Rho) was incorporated in various pH-sensitive nanoparticles. The different patterns of pH-dependent release profiles were observed, although some polymers have the same dissolving pH. The distribution, adhesion and transition of different nanoparticles in rat gut showed significant difference, closely related to the release characteristics of nanoparticles, and their release behaviour are dependent on the dissolving pH and the structure of the polymers, as well as the drug property.Most nanoparticle formulations decreased the distribution and adhesion of Rho in the stomach but increased these values in the intestine. The nanocarriers also control the drug release sites and release rate in the GI tract. In conclusion, pH-sensitive nanoparticles seem favourable for drug absorption and it is important to choose the proper materials to obtain the suitable characteristics for the oral pH-sensitive nanoparticles.
Article: Targeted Treatment of Choroidal Neovascularization Using Integrin-Mediated Sterically Stabilized Liposomes Loaded With Combretastatin A4[Show abstract] [Hide abstract]
ABSTRACT: The objective of this study was to develop an efficient vasculature-targeted liposomal combretastatin A4 (CA4), by the modification of the sterically stabilized liposomes (SSL) with a ligand of integrins and to explore the possibility of such system for the treatment of choroidal neovascularization (CNV). CA4-loaded liposomes were prepared by thin-film dispersion method. The linear arginine-glycine-aspartic acid tripeptide (RGD) with affinity for integrins such as alphavbeta3 expressed on rapidly proliferative vascular endothelial cells was coupled to the distal end of polyethylene glycol (PEG) connected on the surface of SSL. The liposome delivery system was characterized in terms of size and size distribution profiles by dynamic light scattering method, entrapment efficiency, and leakage properties by high-performance liquid chromatography (HPLC). The uptake efficiency by human umbilical vein endothelial cells (HUVECs) was evaluated by confocal microscopy. The therapeutic efficacy was quantitatively assessed by choroidal flat mounts. CA4-loaded RGD-SSL (RGD-SSL-CA4) was obtained with an entrapment efficiency over 70% and an average diameter of approximately 120 nm. The leakage property of RGD-SSL-CA4 was similar with SSL-CA4, both were slower than CA4 ethanol solution. Confocal microscopy studies revealed that RGD-SSL could facilitate the liposomes' uptake into HUVECs. Rats treated with two intravenous injections of 7 mg/kg RGD-SSL-CA4 resulted in a significant reduction in the area of CNV compared with control group (P < 0.05). RGD-modified SSL loaded with CA4 can be successfully prepared, and the vasculature-targeted liposome system would increase the uptake of HUVECs and improve the therapeutic efficacy of CA4 on CNV compared with the control formulations.
Article: Dual-Targeting Topotecan Liposomes Modified with Tamoxifen and Wheat Germ Agglutinin Significantly Improve Drug Transport across the Blood-Brain Barrier and Survival of Brain Tumor-Bearing Animals[Show abstract] [Hide abstract]
ABSTRACT: Chemotherapy of brain tumors remains a big challenge owing to the low drug transport across the blood-brain barrier (BBB), multidrug resistance (MDR), and poor penetration into the tumor tissue. We developed a novel dual-targeting liposomal carrier that enabled drug to transport across the BBB and then target the brain tumor. In the dual-targeting liposomal carrier, tamoxifen (TAM) was incorporated into the lipid bilayer membrane of liposomes and wheat germ agglutinin (WGA) was conjugated to the liposomes' surface. Topotecan was then loaded into the above liposomes. In vitro, topotecan liposomes modified with TAM and WGA were applied to the glioma cells, BBB model, and avascular C6 glioma spheroids, respectively. In vivo, they were systemically administered via vein to brain C6 glioma-bearing rats. In view of the microtiter tetrazolium (MTT) results, topotecan liposomes modified with TAM and WGA exhibited a significant inhibitory effect compared to unmodified topotecan liposomes, suggesting that TAM plus WGA contributed strong drug delivery effects into the brain tumor cells after direct drug exposure. In the experiments of drug transport across the BBB model following drug exposure to tumor cells, topotecan liposomes modified with TAM and WGA exhibited the most robust dual-targeting effects: crossing the BBB and then targeting brain tumor cells. Similar strong activity was found in the reduction of C6 glioma tumor spheroid volume and in the apoptosis of the spheroids. In the brain tumor-bearing rats, the dual-targeting effects of topotecan liposomes modified with TAM and WGA could be evidently observed, resulting in a significant improvement in the overall survival of the brain tumor-bearing rats compared with free topotecan and topotecan liposomes. Moreover, results from an extended treatment group indicated that the survival could be further significantly enhanced, indicating that an extended chemotherapy with topotecan liposomes modified with TAM and WGA would be beneficial for treatment. The dual-targeting effects in vivo of topotecan liposomes modified with TAM and WGA could be related to an enhanced effect by TAM via inhibiting efflux of MDR proteins in the BBB and the brain tumor, and an enhanced effect by WGA via endocytosis in the BBB and in the brain tumor. In conclusion, topotecan liposomes modified with TAM and WGA significantly improve topotecan transport across the blood-brain barrier and the survival of brain tumor-bearing animals, showing dual-targeting effects. These findings would encourage further developments of noninvasive therapy for brain tumor.
Article: RGD-based strategies for improving antitumor activity of paclitaxel-loaded liposomes in nude mice xenografted with human ovarian cancer[Show abstract] [Hide abstract]
ABSTRACT: Tumor-targeting drug delivery systems are being the ideal carrier for systemic administration of antiproliferative drugs. RGD peptide (arginine-glycine-aspartic acid) modified liposomes containing paclitaxel (RGD-SSL-PTX). The arginine-glycine-aspartic acid tripeptide (RGD) modified sterically stabilized liposomes (SSL) containing paclitaxel (PTX) (RGD-SSL-PTX), which could increase targeting to tumor by binding with the integrin receptors overexpressed on tumor cells. The encapsulation efficiency was more than 90% and the mean particle size was of 120 nm with a narrow size distribution. It was indicated that significant cytotoxicity (3.5 times lower IC(50)) was found in the SKOV-3 human ovarian cancer cells treated with RGD-SSL-PTX preparation, as well as the intracellular uptake of liposomes (a 6.21-fold increase in fluorescence intensity), when compared to those of non-targeted liposomes (SSL). For in vivo antitumor activity, it was shown in the present study that RGD-SSL-PTX preparation had the strongest tumor growth inhibition among the test formulations (P < 0.05) in BALB/c nude mice xenografted with SKOV-3 solid tumor. Meanwhile, there was no significant change in the body weight of the animals treated with RGD-SSL-PTX for intravenous injection at a dose of 12.5 mg/kg. It was suggested that the RGD-SSL-PTX preparation might have a great advantage over present-day chemotherapy with Taxol in curing those tumors overexpressing integrin receptors.
Top Journals
Peking University
Peping, Beijing, China
- • State Key Laboratory of Natural and Biomimetic Drugs
- • School of Pharmaceutical Sciences
Peking University Health Science CenterPeping, Beijing, China


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This weekend's rainfall led to flooded roads across New Jersey.
It also led to road closures, including the Blackwell Mills Canal Causeway, right off Canal Road in Somerset.
The rain-swelled Delaware and Raritan Canal spilled over its banks and flooded out a portion of the park along the canal that connects the Delaware and Raritan rivers.
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Barrel Sample. This is a powerful wine, with firm tannins that are well embedded in the ripe blackberry fruits. There is freshness, but the wine also has density and richness. It is a wine for medium-term aging.
This unfolds on your palate with a oyster, raspberry and stone character. It slowly opens and builds. Chewy yet polished. Reserved LMHB.
Taut, with a tangy edge to the red currant, damson plum and blackberry notes, framed with plum skin and blood orange accents and backed by a solid roasted apple wood edge on the finish. This has a rather obvious tannic spine but is not woody in feel. Merlot, Cabernet Sauvignon and Cabernet Franc. Tasted non-blind.
The 2013 La Mission Haut-Brion is intriguing. At first relatively immediate, the 2013 turns darker and more intensely mineral as it sits in the glass. Plum, black cherry, smoke and licorice wrap around the palate with serious density for the year. Bright, floral notes finesse on the finish. The 2013 captures an attractive combination of power tempered by the understated personality of the year. There is a lot to like here. It will be interesting to see if the 2013 gains depth in elevage. The 2013 is 65% Merlot, 32% Cabernet Sauvignon and 3% Cabernet Franc.
(65% merlot, 32% cabernet sauvignon and 3% cabernet franc): Bright ruby-red. Perfumed aromas of floral red berries and spearmint. Refined on the palate, with red berry, mint and mineral elements carrying through to the long, vibrant, but somewhat austere finish. A leaner style of La Mission than usual, but also very refined. If this puts on more flesh it will turn out to be outstanding, but right now it's a little tough, offering no easy sweetness. As with its stablemate Haut-Brion, the percentage of new oak will be cut from the usual 75% to 65% in an attempt to preserve the wine's fruit.
Its big sister, the 2013 La Mission Haut-Brion (65% Merlot, 32% Cabernet Sauvignon and 3% Cabernet Franc) represents 3,000 cases. This very good to excellent 2013 exhibits a deep plum/ruby color as well as notes of subtle barbecue spice, black currants, incense and wet rocks. With good fruit on the attack, medium body and a short finish, it has the potential to be outstanding if it fleshes out. Drink it over the next 10-15 years.
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2013 Lagrange, St-Julien (Pre-Arrival)
2013 Cantemerle, Haut-Médoc (Pre-Arrival)
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2013 Domaine de Chevalier Blanc, Pessac-Léognan (Pre-Arrival)


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Originally posted on Football Nation | Last updated 12/27/12
It looks as though the Ravens are going to proceed with playing their starters this week against the Bengals. With the exception of Haloti Ngata and Marshal Yanda added to the injury list, the Ravens seem to be recovering nicely. Analysts had expressed concern about losing momentum heading into the playoffs and that is the last thing anyone wants. Ray Lewis has already been ruled out for Sunday's game and Terrell Suggs most likely won't play because of the biceps tear. After suffering what was labeled a 'shoulder bruise' on Sunday against the Giants, Anquan Boldin was limited in practice on Wednesday. For the first time in three weeks Bernard Pollard practiced fully. He had been hindered by a chest injury that had kept him out of two straight games. Head Coach John Harbaugh had this to say about Pollard's return. "It was great. Bernard went through the full practice, so he was a full participant in practice. That was great to see. Obviously going forward, he is a big, big part of wh...
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Luke 24:21King James Version (KJV)
21 But we trusted that it had been he which should have redeemed Israel: and beside all this, to day is the third day since these things were done.
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Richard O'Dwyer is heading for trial in the United States. David Cameron must act – now
With so many warm words taking up space in Barack Obama and David Cameron's joint opinion piece published in the Washington Post, there obviously wasn't any room for a mention of the controversial extradition treaty by which British citizens are being handed over faster than quarter-pounders at a McDonald's drive-thru emporium in New Jersey.
Now another Briton, 23 year-old Richard O'Dwyer, a Sheffield Hallam student, will soon be heading for the US in handcuffs. He is accused of running a website which infringed copyright in America. A British court has found that there is no reason for him to avoid extradition under the treaty, and the Home Secretary has given the go-ahead. It follows the recent Christopher Tappin case, and the long-running Gary McKinnon hacking scandal.
This is becoming chilling. What on earth is wrong with ministers? Why are the Conservatives in the coalition so frightened of taking on the Americans? In opposition they indicated that they would be demanding a renegotiation of the treaty but now they shrug their shoulders apologetically.
The Attorney General says in a very lawyerly way that this is all very difficult to resolve. I'm sure it is. But for once I'm with Vince Cable, who says that the treaty is lop-sided in America's favour and that it is imperative that the imbalances are corrected quickly.
Part of the difficulty is the terrible crawling that British governments nowadays feel they must do towards American presidents and governments. They don't want to cause even the slightest fuss or create any unpleasantness in dealings with a much bigger ally who really doesn't think about us very much, if at all.
I say that as a robust Atlanticist. It is no good being starry-eyed about our – what's the word? – "special" relationship. We have interests in common. We have historical ties. We have values in common. But our interests are not always the same as America's interests.
Margaret Thatcher actually understood it. While she supported the US on most of the big questions of her age, she realised that she had far more clout if she were prepared to swing her handbag at key moments (as she did on nuclear disarmament in Europe when Reagan was ready to give too much away).
Tony Blair, being ahistorical and basing his approach to foreign policy on a naive misreading of the Thatcher years, didn't grasp how to do it. The result was disastrous. He said nothing about the lack of preparations for the aftermath in Iraq when he had the ear of the President. Even when it was just Blair, Bush, Condi and Sir Christopher Meyer for dinner at the White House, the British PM didn't realise he had chips to cash in. With the Pentagon running wild, a statement of extreme concern by Blair is about the only thing one can imagine prompting Bush to start asking proper questions of his colleagues.
It should be easier for David Cameron, who is in the US now to visit the President. He's a pragmatic sort and so is Barack Obama.
He must explain that the extradition treaty is of growing concern in Britain. The Government's fundamental duty is to protect its citizens. This treaty does not treat them fairly and, as a result, it is going to have to be overhauled urgently or scrapped.
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We will be using Tisha as the diminutive.
Tuesday, 6 May 2008
Letitia Joy Coles.
5 comments:
She is beautiful!! Congratulations Angela!!ReplyDelete
little tisha is beautiful, angela! i hope you are recovering well and getting enough rest. i can't wait to see more photos!ReplyDelete
Baby cheeks! I love baby cheeks!ReplyDelete
She's beautiful Angela! Congradulations again!ReplyDelete
- Cynthia15 May 2008 at 01:54
...she is sooo beautiful! Congratulations!ReplyDelete
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Hey, I'm Victor Alejandro!
I am an ethical person, with a deep love for the arts. I love learning and discovering all sorts of new things that I can provide people. I studied automotive mechanics, as well as programmer analyst at the technical level, self-taught in electronics. I worked as a mechanic, electronic and data entry. Study for long with intel corp. to get certified as an integrator of Intel products. I consider myself an expert on Intel architecture, I love the compression, cryptology and coding of audio and video. Passionately collect music, movies and digital books. I like to teach others what I know and learn all you can. Long ago I wrote poetry, I consider it very bad. Study piano and marimba chiampaneca at the Mexican Institute of Fine Arts, never finished because I did not have a piano. Stunned dropping out of music, without losing the passion for music. Study theater during high school and be part of two theater groups. I was part of a small group of television production. Collaborate in the creation of one of the first internet radios in Mexico as a personal project. I was the creator of a Japanese anime website in Spanish and English, with material provided by my friends, all for my taste to create multimedia content on the Internet, was the largest Spanish speaking later when a company wanted to buy it, I closed it.
Work as basic technical support and form a true technical support area. Work as a manager and staff only member of the informatics area in a company, to resign, leaving an operational infrastructure that is used today in 3 states (I'm proud), I worked as hacker, cracker, trainer and developer of structures for a business operation VoIP traffic, in order to improve the structure and make it more stable, reliable and practical . Working in a company where we reinvent what you do online, innovating, developing and promoting the use of new technologies. I enjoy the company of my girlfriend and my dog Kyra, a golden retriver. I learn from my girlfriend, this new way to travel and see places starting today. (When most are afraid of change, means that it is necessary).


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I waited with my 71 year old father outside the heavy gates on Via Margutta and phoned Miguel to let us in. Before he arrived, someone walked out so we stepped in. (Note - bring a mobile phone that works!) It's hard to describe this place. I've stayed in a lot of joints in Rome over the years and admit... More
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Monday, February 8, 2016
Sergio, thirty, works in a big supermarket. His wife Angela, born in Spain, is expecting a child the doctors have diagnosed as having a serious malformation. The child is not expected to live. When the baby is born, he's baptised Miguel Juan, in honour of an inhabitant of Angela's home village who had received a miracle. Angela prays for her child. Sergio, lacking his wife's faith, is plunged into desperation. This is compounded by his implication in the investigation into the rape of a young woman who had taken her own life after the violence. While gathering information about Sergio, police Commissioner Sironi gets involved in the events concerning Miguel Juan: the fate of the newborn baby catalyses existential tension and anguish, breaking down both Sergio and Angela. ~ Baseline StudioSystems


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Barefield, Ennis, Co Clare
Phone: 065 6827151
Email: email@example.com
Web: www.elmdalehouse.com
Elmdale is a modern Georgian house which is very convenient to castles caves Cliffs of Moher the Burren and Shannon Airport. Meals are on request and there is a resident harpist present. Farm walks are a speciality in this property.
Experience Irish hospitality at it's best at Elmdale Farmhouse B&B with a resident harpist and Irish Step Dancing in our home. We are located 8km from Ennis town on the N18 Route. Elmdale is a perfect base for touring the delights that County Clare has to offer including the Cliffs of Moher, the Burren region and the Aran Islands. You can enjoy country living at it's best, on our farm with 120 acres with impressive surroundings. A delightful welcome awaits you here with home cooking daily.


text:
We have also tripper from trip, a dialect word for flock, probably related to troop.
A week-end tripper, or somebody with a flourish at each end of his name?
tripper (who is proud of his superior acquaintance with a foreign language).
tripper was standing beside him, and pointing at something broad away on the beam.
The best example of the cultured, but common, tripper is the educated Englishman on the Continent.
The day tripper, with his Arriet, is unknown here, for the simple reason that there would be nothing for him to do.
To a mere outsider—a mere summer tripper hurrying through—Yarrow is little different from others of the southland valleys.
But the tripper is not welcome and the envious tangle of trees and shrubbery denies to the wayfarer even a glimpse of the house.
In the twilight the blush caused by being taken for a Plymouth tripper was not perceived. '
tripper—none too clean in appearance—charters bathing machine.
An area in Manhattan being developed as an artists' and residential neighborhood: They've traipsed all over Lower Manhattan, from Greenwich Village to Tribeca
[1980s+ New York City; fr triangle below Canal Street]


text:
Tyrone Keith McGriff
Position: G
Height: 6-0 Weight: 270 lbs.
Born: January 13, 1958 in Vero Beach, FL
Died: December 9, 2000 in Melbourne, FL
High School: Vero Beach
College: Florida A&M (school history)
Drafted by the Pittsburgh Steelers in the 12th round (333rd overall) of the 1980 NFL Draft.
Weighted Career AV (100-95-...): 8 (7844th overall since 1950)
Promote your website or business by sponsoring this page for $5 on Pro-Football-Reference.com. Your message will replace this ad.


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Welcome to KIDS U!
Summer fun and learning on the University campus with seven weeklong camp sessions, June 13-July 29, 2016!
Registration opens March 19!
For Parents
NEW Registration Instructions
Online Registration:
• Grades 2-4 opens at
8 a.m., March 19
• Grades 5-8 opens at
10 a.m., March 19
2016 KIDS U Brochure
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Health Form (mandatory)
Pledge Form (mandatory)
Parking Permit and Map
Payment Schedule
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To view and fill PDF files, you will need to use Adobe Acrobat Reader, a free program. Click to download:
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University of Nevada, Reno
Extended Studies
Redfield Campus
Nell J. Redfield Building A
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Reno, NV 89511
(775) 784-4062 or 1-800-233-8928
Please note: Our offices are now located on the Redfield Campus in south Reno. The 2015 KIDS U sessions are held on the main university campus.


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Animal Love, adorable but contagious!
Photo courtesy David Brough Watch out! Love in the animal world is going around... and it's catching! Mild symptoms of contagion might just be a smile on your face, but in worst case scenarios you may experience uncontrollable laughing, or even guffawing. And smiles, too, are contagious to other people! Being an animal lover, however, is perhaps one of the best epidemics imaginable! Animals and pets are good for your mind, good for your body, and good for your spirit. Studies have shown that keeping pets relieves depression, improves marriages, and kids with pets tend to function better. Caring for a pet also ...
Fairy Wrasses, Creating an Aquarium Fantasy World
Animal-World proudly features: Fairy Wrasses, an aquarium fairyland at its best! These gorgeous fish came into my life shortly after I started into the saltwater hobby. Fairy Wrasses quickly became my personal favorite finned fish when they first began to appear many years ago. They have held a special place in my heart ever since! Initially Fairy Wrasses were not too expensive but then prices started to soar for a few years. Now they are at a moderate to very high price, depending on the species you want. Still they never disappoint and each species seems to have a little ...
The Spotted Snake Eels
Animal-World's Newest Featured Animals are: The Amazing Spotted Snake Eels! Out of all the salt-water fish I have ever owned, I have to say the Snake Eels of the Myrichthys genus have always been my favorites! Watching them bury under the sand, leaving just their head sticking out, is the funkiest thing you'll ever see. But the coolest part of owning them is that they will eat silversides right from your hand! And if they accidentally grab your finger, it never hurts becuase they grab and swallow their prey rather than biting it. I will refer to these as ...
The Chinchilla
Animal-World's Featured Pet for this week is: The Chinchilla! I have to admit, one of my all-time favorite pets has been the Chinchilla. I didn't start owning them until I was in high school, but after I got my first one, I was in love! I have owned 5 Chinchillas since that first one! Many people who keep chinchillas as pets will attest to their wonderful pet qualities. A niche market for chinchilla fur also exists and chinchilla breeders around the world abound for this very purpose. Chinchillas are believed by many to be wonderful and personable pets. Being very loving and ...
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Photo courtesy: Lisa Stanley Folly Guinea Pigs may come up when your kids want a pet, and of course, you'll want a good pet that's good natured and easy to care for! Still they can pose the question "What the heck are they?" Even though guinea pigs are familiar, they're neither pigs nor do they hail from Guinea. They are... [Read more of this article]Choosing a Kennel to Board your Pet
Alternative care for your adorable pet is stressful on many levels, but it helps to think ahead! It happens, you have to leave town! You have to be away on business, a family emergency, or even on vacation. You may be gone for a day, a week or two, or maybe even longer, so now comes the big question. Who will care for your kitty, dog, or other pet... [Read more of this article]Birdwatching delights, Atlantic to the Mediterranean
Beautiful and exotic birds can be seen from the coasts of Western Europe to the Mediterranean Sea! Gorgeous forests and wetlands from the eastern Atlantic to the Mediterranean, offer fantastic viewing opportunities for birdwatching enthusiasts and nature lovers alike! What could be more enchanting than a visit to the Formosa National Park! This protected... [Read more of this article]The Blue Devils, stunning but pugnacious damsels!
Many of the Chrysiptera damsels are lavishly colored, but the two Blue Devils top the list of favorites! The splendid, eye-catching appearance of these Blue Devil's simply can't be ignored. Despite having a bit of the "devil" in their personalities, these two damselfish are extremely popular with aquarists. The marvelous electric... [Read more of this article]Five Tips for taking care of your cat
How to take care of your cat properly, here's 5 great tips! Having a pet has many advantages and can help you develop a rewarding relationship with one or more animals, but they depend on you for grooming, feeding and taking care of them. Cats are among the most popular pets, and because they are rather independent, taking care of them is not... [Read more of this article]Best Dog Toys Of 2015
When it comes to keeping your furry family member happy and healthy, a dog toy provides hours of endless entertainment! Keep your pet safe by choosing the best and safest toys. The Humane Society notes to choose toys best suited for your dog's size and activity level. Furthermore, purchase toys especially made for dogs rather than giving your pet... [Read more of this article]View All Posts


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Kelly Rutherford and a Few of Her Favorite ThingsWhit Honea
It's beginning to look like Christmas. A LOT. Time to break out the sweaters and the Bing Crosby collection.
Then there is Kelly Rutherford and her daughter Helena who get seasoned up by paying a visit to the Saks Fifth Avenue Holiday Shop in NYC. Ah, autumn in New York.
By the way, this year 5% of all candy and food sold in the shop will benefit St. Jude's Children's Research Center, which is a nice touch. St. Jude's is good people.
Photos courtesy of Saks Fifth Avenue
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Article Posted 6 years Ago


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Phoenix Suns Blog
Summer League: Miles Plumlee stuffs Jabari Parker
Posted on
Jul 14, 2014
Miles Plumlee's block on fellow Dukie Jabari Parker on Sunday night wrapped up what was a telling game in his second outing of the 2014 Las Vegas Summer League. After an ugly first time out,...
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STARmeterSEE RANK
Up 2,310 this week
Savannah Guthrie
Savannah Guthrie was born on December 27, 1971 in Tucson, Arizona, USA as Savannah Clark Guthrie. She is an actress, known for Today (1952), 2014 Golden Globe Arrivals Special (2014) and Saturday Night Live 40th Anniversary Red Carpet Special (2015). She has been married to Michael Feldman since March 15, 2014. They have one child. She was ... See full bio »
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Ma'anit by BonvanelloWatch
©2014-2016 Bonvanello
This is brilliant!
I LOVE the landscape! It looks so realistic and the atmosphere is just perfect. Wonderful work!
Wow! These mountains you created are amazingly detailed and so very realistic. What a beautiful scene overall. I really love the hazy atmosphere, like there's a sand storm that had just blown through, clearing to reveal the mysterious planet in the sky. I want to explore this place -- it seems quite real. Great work, my friend!
Thanks buddy. Love your comment
I was trying to create something that looks somewhat realistic, lol
And you know I always have to add a planet to it.


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Luke 24:32New International Version (NIV)
32 They asked each other, "Were not our hearts burning within us while he talked with us on the road and opened the Scriptures to us?"
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AP Tennis Headlines At 11:30 p.m.
Updated: June 25, 2013, 12:32 AM ETAssociated Press
Nadal out of Wimbledon in 3-set stunner
Azarenka hurts knee in opening victory
Sharapova struggles but tops Mladenovic
Hewitt upsets 11th-seeded Wawrinka
Murray eases into 2nd round at Wimbledon
Isner wins Wimbledon opener in 3 sets
Puig, 19, stuns No. 5 seed Errani
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Edmond Memorial RB Warren Wand's recruitment remains open
Edmond Memorial running back Warren Wand isn't ready to say he's verbally committed to Arkansas State.
It was reported nearly two weeks ago by various media outlets that Wand had committed to the Red Wolves, but Memorial coach Justin Merideth told The Oklahoman on Friday his star running back hasn't made that decision, though a decision could come as early as next week.
"He's not ready to let anybody know," Merideth said.
Wand, who is No. 11 on The Oklahoman's Super 30, also has offers from New Mexico and Southern Mississippi.
He rushed for 1,769 yards and 15 touchdowns last season, leading the Bulldogs to the Class 6A playoffs for the second straight season.
But he's also recovering from a quad injury he suffered during track season. He has yet to practice this week for the Bulldogs, but is expected to be ready in time for the season.
Wand was unavailable for comment.


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Monkey see, monkey do your classroom decorations right with a Colorful Sock Monkey theme! Let these awesome resources swing you into action this school year with high energy and contagious excitement.
Find every decorating and organizing need for a colorful Sock Monkey makeover.
Create a bulletin board that will capture attention, promote conversation, and foster an atmosphere of learning with our collection of trimmers! From neutral to neon and everything in between, we have a selection to match every theme. And with our everyday low prices, you can update your boards as often as you like!
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Are there any Guardians that will be playing on PS4 that have a headset of some sort. The majority of people on PS4 never use a headset and I want to have Fireteams that I can talk with. If your going to play on PS4 and have a headset feel free to add me at jaypaw28
e7r5i1k10/13/2014 5:09:02 AM PermalinkAdding you, baby. Hunter 28 e7r5i1k Agree with everything you said and have been looking for bastards.
DaFisHMINaT0R9/15/2014 7:19:03 AM Permalinksick as - oldhttp://www.bungie.net/en/Clan/253059 CRIT HARD OR GO HOME. COME JOIN US MATES REALM OF CRAZY AUSSIES FOR PS4 destiny come join us Destiny REALM of CRAZY AUSSI3S [RCA] "CRIT-HARD OR GO HOME" Mission Statement Aussie casual clan starting with mates & looking to expand We will be doing pvp,raids,fireteams,strikes & events When these start they would be weekends or at night where we will make certain nights!!! ATM enjoy destiny & level up & chase ya gear once we have eough members n classes n gear we will start on raids !! We are looking for titans ATM But all esle are able to apply DaFisHMINaT0R Founder **** Australian ps4 players**** The REALM of CRAZY AUSSIES ARE Recruiting for raids,strikes,missions n pvp
Nusevad9/10/2014 2:21:21 PM PermalinkFeel free to add me Psn: Nusevad I have a mic I play on EST usually ard 8pm
Goose22629/10/2014 2:15:19 PM PermalinkMy puppy just chewed my mic so i will be replacing within the week. Add me
SPQR Soul3vans9/8/2014 9:48:44 PM PermalinkIf you're looking for a clan, SPQR Legion is always on the hunt for new blood. We're home to both serious and casual players alike, focused more on creating a positive community of gamers than anything else. If you feel the call to join mighty Rome in her conquest of a new world, you're welcome to message me. STRENGTH AND HONOR! -Centurion Soul3vans
TheBronzeBull9/8/2014 9:18:33 PM PermalinkPSN: TheBronzeBull Mic: Yes Time Zone: Eastern US Age: 21 Looking for a coordinated group of people with mics to have some fun!
Vindizzl39/8/2014 4:35:31 AM PermalinkVindiezl3. The more friends the merrier. Feel free to add me
CrimDelaCrimm9/8/2014 4:19:17 AM PermalinkYes I will feel free to hop on and play with me. I'll be on a lot at launch. PSN: CrimDelaCrimm
SteepHyperspaceEdited by SteepHyperspace: 9/6/2014 10:44:15 PM PermalinkSend me a PSN message or friend request (Username: SteepHyperspace) or join the [url=http://www.bungie.net/en/Clan/Detail/181659]'Keepers of the Jungle'[/url] group on bungie.net if you are considering enlisting. You will have people to play Story Missions, Strike or Patrol. If (in the unlikely event) we get 6 or more players to join, we could have Raid Missions or Crucible Matches organised. Also, I have devised a clever little, military grade (not really) fire-team system, applicable to a team of 6 players (if we get there). Anyway, can't wait to see you all on the 9th! GMT/BST only.
Doobiewan Kenobi9/6/2014 9:37:54 PM PermalinkPSN: doobwan - got headset read for raids and strikes
whisperingsloth9/5/2014 5:17:38 AM PermalinkPsn:whisperinsloth If anyone wants to join a small fairly social and laid-back clan with no pressure and just likes to have fun then Immortal Resistance has openings, we look forward to raids also, see you on the battlefield guardians :-) http://www.bungie.net/en/Clan/Forum/176500


text: RT Web Page DB /z-wcorg/ DS http://worldcat.org ID 439387983 LA English UL http://www.mitfordbooks.com/ T1 Jan Karon PB s.n. PP S.l. YR 199u AB This Web site is the official on-line resource of bestselling fiction author, Jan Karon. Come and explore Karon's talent at illuminating the struggles that creep into everyday lives. Visitors can read Karon's biography, gain insight into her writing style, and find out what she's currently working on.


text:
Knoxville (AP) -- Financially troubled Knoxville College has lost its president-elect.
The chairman of the college's board of trustees confirms the college last week agreed to end its contract with Earl Yarbrough, Senior.
Instead of coming to Knoxville, Yarbrough will take the presidency of Savannah State University, the oldest of Georgia's historically black colleges.
Yarbrough was to take office at Knoxville College this month after being selected by the board in December. He's leaving a post as professor and former provost at Virginia State University.
Yarbrough told the Knoxville News Sentinel the issue at Knoxville College wasn't just his salary. Yarbrough said he was he was concerned there wouldn't be enough money to pay faculty and staff.
In the spring semester, Knoxville College had about 200 students.
(Copyright 2007 by the Associated Press. All rights reserved.)


text:
- 81Promote
Throw Pillow
Breatheby Sandra Arduini
Throw Pillow made from 100% spun polyester poplin fabric, a stylish statement that will liven up any room. Individually cut and sewn by hand, each pillow features a double-sided print and is finished with a concealed zipper for ease of care. Sold with or without faux down pillow insert.
So lovely! Beautiful work :)
love ♥
very nice!
So pretty!!
Thank you Josrick, Christy, Sylvia and Kim!
beautiful work!
Breathe...beautiful Sandra!!!
love it!
Love this, Sandra! Beautiful!
Thanks everyone! :)
Oh Yeah! ✯ ✯ ✯ ✯ ✯ 👍
See More Comments


text:
Ask the Experts
consequences of snorting cocaine (HEPATITIS C and SNORTING COCAINE, 2010)Hi,I snorted cocaine for the first time last weekend, using a shared dollar bill. My nose didn't bleed and I didn't notice any blood on the note. I definitely won't do it again, but I have become really anxious as a consequence of using it. My legs...
Response from Robert J. Frascino, M.D.
The Robert James Frascino AIDS Foundation...first to have these fears. See below. I have nothing new to add.Dr. BobShared Cocaine Snorting Straw With Someone, and Worried (HEPATITIS C and SNORTING COCAINE, 2008)Oct 27, 2008About two weeks ago I shared a straw to snort cocaine with a friend... Read more »
Dr. Bob, can i woohoo, please answer? Will donate. (HEPATITIS C AND DELAYED HIV SEROCONVERSION, 2010)Dr. Bob, I have had one time unprotected sex with a stranger in the past, she told me that she had... Read more »
Confused after reading some Hep C and HIV answers and questions!(HEPATITIS C AND DELAYED HIV SEROCONVERSION, 2010)Hi, Doctor, I had written to you previously and made a donation of $50, all I could afford, sorry. ... Read more »
California: Cal State-Long Beach Gets Grant to Study Faster HIV, Syphilis, Hepatitis C Tests...recently awarded California State University-Long Beach a four-year, $1.7 million grant to study experimental rapid tests for HIV, hepatitis C, and syphilis. The project will assess the accuracy and acceptability of the tests among groups at high... Read more »
Hepatitis C Laboratory Tests...Tests HCV Genotype Tests IL28B Genetic Test Blood Clotting Tests Several laboratory (blood) tests are used for hepatitis C (HCV). These include liver function tests, HCV viral load, genotype tests and blood clotting tests. Liver... Read more »
This Month in HIV: Update on Hepatitis C/HIV Coinfection... Welcome to This Month in HIV. I'm Terri Wilder . This month's topic is HIV and hepatitis C coinfection. Up to one third of people with HIV are estimated to also have hepatitis C, and living with both viruses is no picnic. Read more »
FAQs for the Public: Hepatitis C... Overview What is hepatitis? What is the difference between hepatitis A, hepatitis B, and hepatitis C? What is hepatitis C? Statistics How common is acute hepatitis C in the United States? How common is chronic Read more »
Connect With Others
Hepatitis C
Posted by hopefulindy, 1 Reply...I tested HIV- at 3.5 months post possible exposure. I have on going health issues and no answers. I am waiting on blood work to see if Read more »
Hepatitis C
Posted by capeac, 1 Reply...no tengo de nuevo hora para la consulta y me gustara saber que sabeis sobre la fiabilidad de los resultados en el test de la Hepatitis C. ?Es cierto que cabe la posibilidad de un falso negativo -ojo estoy hablando de la Hepatitis C no del VIH-... Read more »
When should I test?
Posted by Shauna99, 7 Replies...but there was one or two times when it just happened. We last had sex about 2 months ago. I need to know when I should be tested. There seems to be conflicting information on the internet about 3 months or 6 months. Is it better to wait for... Read more »
cocaine hiv and hepatitis c
Posted by Anonymous, 2 Replies...LOOKED AND IT HAD BLOOD IN AND AROUND THE STRAW. I HAD A COLD SORE IN MY NOSE. THE GIRL CALLED ME ;ATER AND TOLD ME HE HAS HEPATITIS C AND HIV CALLED CDC AND THEY SAID IT COULD BE POSSIBLE SHOULD I GET TESTED HAVE YOU EVER HEARD OF CATCHING HIV... Read more »


text:
Jaycees - Indianapolis
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text:
The Star Harpy Ep 3The Star Harpy Ep 3 by BluGail
Nick was holed up in the engine room. There were three guards outside taking pot shots at him, and one laid out cold on the floor inside. Nick had his rifle.
One of the guards called out, "You're just making this harder on yourself. Surrender now, and we'll do you quick and painless."
Nick called back, "That's not enticing me. How about we all stay here with the engines shut down, until more of my friends arrive, and then I do you, real quick and painless?"
A hand grenade bounced through the doorway and landed next to the unconscious guard's head. It was out of view of the corridor, so Nick stepped in and kicked it back through the door. A second later, there was a bright flash and flames shot up to the ceiling, a flame grenade. Nick peered into the corridor to see the guards bent over, covering their eyes. He took careful aim, and shot one in the leg.
"F**k!" The guard yelled out
"Are you okay?" Another guard asked.
"F**k no, he shot me in the leg! Motherf**ker, I'm gonna f**king kill
The Star Harpy Ep 2The Star Harpy Ep 2 by BluGail
The shuttle touched down on whichever random public dock happened to be open near the casino, so there wasn't much risk of being picked up by Demetri's men as long as they didn't linger.
Sydney felt naked stepping off the ramp. Not just because she barely had any clothes on, but because it was the first time she'd been out in public as a catgirl.
Human-animal hybrids weren't unheard of, most people had seen them on the net, and there were a few celebrities who had had it done, but it was still unusual to see them walking around on the street. Most were either exotic prostitutes, circus performers, or rich kids with too much money. As far as civil rights went, they were afforded all the same protections as full humans, but some people preferred not to interact with them, calling them perverts or deviants.
She kept watching to see if anyone was staring at her. A few people glanced her way, but just for a moment. She was near the casinos and in that kind of place, with shows, hookers, and
The Star HarpyThe Star Harpy by BluGail
It'd been a lousy night for poker. Tal Rommi was only up seventy bucks, and the only thing that made that remotely worthwhile were the free drinks she was getting. It was late, and with most of the waitresses on their way home, the service bots were coming out to take the night shift.
Sure the bots were made to look like drop-dead sexy girls covered in chrome, and some of the customers preferred them, but they were lacking the human element. Plus, the waitress Tal had been making eyes at for the last two hours, the one that kept bringing her stiff drinks, had disappeared.
Tal wondered if she was really as sexy as she thought she was. Maybe she should have worn something sluttier? but the old skin tight flight suit had always worked well for picking up little morsels like that waitress in the past. Confidence is the key, but showing off the goods couldn't hurt either.
She closed her eyes and accessed her bio menu. She left the hair alone, burgundy was her best color anyway, and shifted


text:
Jin Kazama by surberus666Watch
©2013-2016 surberus666
selfie that i took before our tekken shoot
DUDE are you sure your real. you are HOT
You nailed it perfectly Jin-sama! Your features did it right. If jin was a martial arts teacher I'd enroll myself just to see him everyday. Great Cosplay amazing
That hair O.O
oh wow amazing
omgg <3
thank you so much! i love your cosplays specially poison from street fighter
The first time I saw this pic, I was freaking screaming! XD My gosh!!!!! YOU LOOK AWESOME! XD I totally love you now! XD
Lets Fight TEKKEN at XBOX!
Your face is so perfect for the cosplay of Jin
You are good-looking man!!! Why is it that all the good man live halfway around the world????
I think I'm in love.
do you play the game?
i was addicted to tekken tag for 3 years i think
perfect mix of hot and cute
Very nice!
Dat sexiness...
Look sexy
STAPH being so cute <3
you are and adorable Jin <3


text:
FOUR HUNDRED + SEVENTY NINE by anita-trimburWatch
©2012-2016 anita-trimbur
Wonderful kitty capture!
So cute!
Also, updated to a higher resolution and made it into a print.
Beautiful shot and subject! You did a great job with the back ground, it's classy but has other colors/subjects that are complementry but not distracting. I feel like the cats face is a little too... sharp/detailed in comparison to everything else though. But it's really a great peice, good job.
Very interesting eyes...!
beautiful cat, beautiful photo :] I love it btw. thanks for fav
So freaking gorgeous


text:
Britney Spears
Britney Spears
With no head-shaving incidents to point to this year, the only Britney madness we have is the fact that she has a new album out which is underwhelming reviewers, and that she and K-Fed are possibly planning to give their relationship (and their marriage) another shot.
While this might signal a happy ending for Brit and K-Fed's two boys, we would like to urge our girl to really assess, through group and individual therapy if possible, whether she and K-Fed are right for each other, or if this is a partnership of convenience, so neither has to get back out there in the speculation spotlight. While angst often breeds better music, resolution of your issues raises healthier children in the end...at least until little Sean Preston's friends start making fun of his mom for kissing Madonna on TV.
Why Practicing 'The Jesus Prayer' Is So ImportantLord, Jesus Christ Son of God, have mercy on me a sinner.
4 Unnecessary Things You Don't Need to be Healthy...We are loaded with medical news and information every day. Some of the information...
4 Unbelievable Angel Sightings Caught on CameraEven the toughest skeptics would have a hard time denying that angels exist...
More from Beliefnet
Celebrity and Pop Culture News from Idol Chatter
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text:
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CPCF Faculty

SBH 310, x4145 or SBH 329, x6352
email@example.com
Winter 2016 office hours:

Nick Baxter-Moore (PhD Carleton University) focuses his research on popular culture and identity; class, nation, and popular music; local popular culture; and live performance. He teaches courses on popular culture, popular music, media industries, and research methods. Nick is also the Associate Dean for Undergraduate Studies for the Faculty of Social Sciences. Learn more about Nick.

SBH 338, x5364
email@example.com
Winter 2016 office hours: Mon. & Thurs. 10:00am -11:00 am

Jackie Botterill (PhD University of East London) explores cultural economy, consumer culture, advertising and promotion, and consumerism and lifestyle, as well as status, class, identity, and marketplace politics. She teaches courses on communication theory, organizational culture, cultures of consumption, and mass culture. Her most recent book, Consumer Culture and Personal Finance: Money Goes to Market, was published in 2010. Learn more about Jackie.

SBH 322, x3180
 firstname.lastname@example.com
Winter 2016 office hours: By appointment

Dale Bradley (PhD York University) teaches classes on technology and culture and the social history of information technology. Dale's research interests lie in the popular representation of cyberculture, the impact of information and communication technologies on office culture and workspaces, and political discourse on the open source software movement. Dale is on sabbatical until July 2015. Learn more about Dale.

SBH 314, x4658
email@example.com
Winter 2016 office hours: By appointment.

Marian Bredin (PhD McGill University) teaches courses on Canadian communications and cultural policy, media and minorities, media analysis, and Canadian television. Marian's research examines Aboriginal media, representations of cultural difference, and the connections between heritage, tourism, and popular culture. Marian is on sabbatical until July 2015. Learn more about Marian.

TA 348, x3898
 firstname.lastname@example.com
Winter 2016 office hours: Tues. & Thurs. 3:30-5 p.m. or by appointment

Sharon Broderick is a cross-appointed faculty member who also teaches in the Goodman School of Business and the International Masters of Accountancy. For CPCF, Sharon teaches courses in public relations and integrated marketing communications. Learn more about Sharon.

SBH 313, x5365
firstname.lastname@example.com
Winter 2016 office hours: Tues. 1:00 p.m. - 3:00 p.m. or Wed. by appointment

Tim Dun (PhD University of Iowa) teaches courses on interpersonal communication,communication theory, organizational communication, negotiation and conflict, gender and communication, and public speaking. His research areas include interpersonal conflict, communication theory, and intergenerational family relationships. Learn more about Tim.

SBH 311, x5366
firstname.lastname@example.com

Winter 2016 office hours: Currently on Sabbatical until July. Office hours by appointment only.

Derek Foster (PhD Carleton University) teaches courses in visual rhetoric, audience research, language and power, theories of the media, and the rhetorical communication of social issues. Derek is interested in visual rhetoric, television studies, discourse analysis of public culture, and material popular culture (including mediatized place promotion). Learn more about Derek.

 SBH 308, x3160

 firstname.lastname@example.com
Winter 2016 office hours:

Greg Gillespie (PhD University of Western Ontario) teaches and researches popular culture with a focus on non-mass mediated subjects (including role-playing games, sport culture, and Scottishness). His recent publication Barrowmaze II, placed second for the 2013 Three Castles Design Award for RPGs. Learn more about Greg.

SBH 342, x3707
firstname.lastname@example.com
Winter 2016 office hours: Tues. 1:30-2:30 p.m., Thurs. 2-3 p.m. and by appointment

Jennifer Good (PhD Cornell University) teaches classes on environmental communication, mass media effects, research methods, and communication theory. Her research focuses on the places where mass media (especially television), materialism, and our understanding of/relationship with the natural environment intersect. Learn more about Jennifer.

SBH 341, x3215
email@example.com
Winter 2016 office hours: By appointment.
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Barry Grant (PhD State University of New York at Buffalo) is interested in film genres, documentary film, horror and science fiction, popular music, and popular culture. He has published extensively in many areas of film and popular culture; his most recent publication is a British Film Institute Screen Guide entitled 100 Science Fiction Films. Learn more about Barry.

SBH 340, x4287
email@example.com
Winter 2016 office hours: Please contact for appointment.

Scott Henderson (PhD University of East Anglia) teaches classes in theories of popular culture, popular cinema, popular music, and film theory. Scott's research explores music and locality, youth culture, popular culture and identity, British cinema and popular culture, and Canadian popular culture. Learn more about Scott.

SBH 320, x3154
firstname.lastname@example.com
Winter 2016 office hours: Tues. 9-10:30 a.m.

As a researcher, Russell Johnston (PhD Queen's University) is most interested in media history and popular memory. He teaches classes on cultural industries, the history of advertising, sport media, and research methods. In 2012, he co-wrote the Canadian edition of Communication Research Methods. Learn more about Russell.

SBH 335, x6397
email@example.com
Winter 2016 office hours: Tuesdays, 11am-1pm.

Anthony Kinik (PhD McGill University) is a film studies scholar whose areas of specialization include documentary film, experimental film, theory, authorship, and Canadian and Québécois cinema. His most recent publication was titled "Celluloid City: Montreal and Multi-screen at Expo 67," Reimagining Cinema: Film at Expo 67 (McGill-Queen's University Press 2014). Together with his colleagues Steven Jacobs and Eva Hielscher, he is currently working on a manuscript with the working title Montage Metropolises: The City Symphonies Phenomenon (Routledge 2017).

SBH 307, x3822
firstname.lastname@example.com
Fall 2015 office hours: by appointment.

Peter Lester (PhD Concordia) studies Canadian film history, screen culture, film technology, exhibition studies, and non-theatrical film. He teaches courses in Canadian cinema and documentary film. Learn more about Peter.

SBH 332, x4395
 firstname.lastname@example.com
Winter 2016 office hours: Fri. 2-3 p.m.

Sarah Matheson (PhD University of Southern California) teaches courses in television studies, Canadian television, popular culture theory and research methods, and taste and cultural politics. Her research is in the area television studies with particular focus on Canadian television. Learn more about Sarah.

SBH 336, x4480
 email@example.com
Winter 2016 office hours: by appointment

Christie Milliken (PhD University of Southern California) researches sex and gender in cinema, sex education film and video, documentary studies, and pedagogy and film. She teaches classes in gender and film, documentary history and theory, American and international film history, cult cinema and fan culture, and melodrama studies. Christie is on sabbatical until July 2015. Learn more about Christie.

SBH 334, x5221
 email@example.com
Winter 2016 office hours: Currently on Sabbatical until July. Office hours by appointment only.

Bohdan Nebesio (PhD University of Alberta) teaches classes in film history, Hollywood cinema, classical film theory, and national cinemas. His research interests include film history and the history of film theory, silent cinema of the 1920s, cognitive approaches to film studies, cinemas of Eastern Europe, and Alexander Dovzhenko. Bohdan's most recent book, Historical Dictionary of Ukraine (2nd Ed.), was published in 2013. Learn more about Bohdan.

SBH 316, x4065
firstname.lastname@example.com

Joan Nicks is a retired faculty member whose main research interest is the local popular culture of Niagara Falls. She is currently the editor and project coordinator of a manuscript in progress entitled Niagara Movie Theatres and Motels: Cultural Loss and Decline in Tourist City.

SBH 316, x4065
email@example.com
Winter 2016 office hours: Wed. 1:30-3 p.m.

Jeannette Sloniowski (PhD University of Toronto) teaches courses on
television, popular narrative, film genre, film noir, Canadian detective fiction, and film and television of the 1950s. She has also been involved with Popular Culture Niagara, a departmental research group that studies various aspects of local popular culture. Her research focuses on crime fiction, cop shows, private eyes and documentary, as well as local popular culture and popular narrative. Learn more about Jeannette.

SBH 307, x6497
Winter 2016 Office hours:
email@example.com

Karen Smith (PhD University of Toronto) conducts research which explores the themes of openness, privacy and participation in digital society. Karen teaches courses related to social media, user experience, surveillance, and new media policy issues. Karen is published in journals such as Surveillance & Society, and collaborates extensively with Hive Toronto, a Mozilla stewarded digital literacy network.

SBH 343, x6705
email@example.com
Winter 2016 office hours: Tues. 10-11 a.m. or by appointment

Laura Wiebe (PhD McMaster University) teaches courses on popular music, popular culture, technocultures/digital cultures and cultural theory. Her current research investigates cultural identities in popular music scenes, focusing on the case of the Quebec metal scene of the 1980s. Laura has also researched and published in the area of science fiction and fantasy studies, examining representations and constructions of modernity, science, technology, gender and difference in speculative narratives across media.

SBH 337
firstname.lastname@example.com
Winter 2016 office hours: by appointment

Andrea Zeffiro (PhD Concordia) teaches classes on digital literacy, and communication and media studies. Her research intersects the cultural politics and practices of emerging technologies, contemporary media histories, and art activism. Learn more about Andrea.

Amanda Bishop's office hours: By appointment
email@example.com
SBH 318

Bill Boehlen's office hours: By appointment, booked via email
firstname.lastname@example.com

Greg Jackson's office hours: By appointment
email@example.com

Tracy Kennedy's office hours: By appointment
email@example.com
SBH 337

Patti Lemene's office hours: by email request
firstname.lastname@example.com
SBH 337

Glen Norton's office hours:
firstname.lastname@example.com
SBH 337

Sarah Posen's office hours:
email@example.com
SBH 320

Jane DeMan
Administrative Assistant
Scotiabank Hall 309
(905) 688-5550 ext. 4290
firstname.lastname@example.com

Penni Lafleur
Academic Administrator
Scotiabank Hall 344
(905) 688-5550 ext. 5308
firstname.lastname@example.com
Book an Advising Appointment:
1. Through Student Self-Serve
2. Email
3. Phone

Desirae Stack
Coordinator (Experiential Learning Course, MA Pop Culture, BUFS)
Scotiabank Hall 310
(905) 688-5550 ext. 4394
email@example.com

Source URL:


text:
Lester 4ladstone /ugbee
I N 1896 -at the beginning of the second term of the University
of Texas, I for the first time became a denizen of B Hall,
where L. G. Bugbee was also in residence under the supervision
and careful scrutiny of H. B. Beck, the first manager of that
famous hostelry. I am not sure whether I first met Bugbee in
B Hall or in the United States history class which he was then
teaching in lieu of Professor George P. Garrison, absent in
the University of Chicago to finish his work for the degree of
doctor of philosophy. But I do recall that from the first we
met on common ground: he had grown to manhood among the
post oaks and black jacks of the Cross Timbers of Johnson
County, and Johnson County is fringed on the southwest by
the Brazos River, just across which lies Bosque County, where
I grew to manhood. I think he was the first student from his
county to discover the University of Texas. I know I was the
second to enroll from Bosque County.
Bugbee, young and inexperienced as a teacher, had a difficult
role to play in following in this history course Professor
Garrison, a masterly teacher. The class was made up of juniors
and seniors. I remember how Bugbee's unassuming modesty,
his quiet competence, his friendly attitude quickly won the
confidence and esteem of twenty or more hypercritical upper-
classmen. Secretly perhaps, but with absolute sincerity, I
became his admiring friend. Perhaps in him I saw the possi-
bility of a country boy's achieving distinction even amid the rigid
standards of competence that characterized any respectable
so-called institution of higher learning. At any rate, growing
out of this contact and of my becoming registrar of the
University in 1897, Bugbee often talked to me of his plans for
seeing the University a depository of all forms of historical
material - letters, diaries, old and neglected annals like the
Bexar Archives (which I believe he secured)---any written
records that threw light on how Texas came to be or showed
how Texas had grown. Our acquaintance gradually ripened into
One day he came to me with a proposition that brought us
Texas State Historical Association. The Southwestern Historical Quarterly, Volume 49, July 1945 - April, 1946. Austin, Texas. The Portal to Texas History. http://texashistory.unt.edu/ark:/67531/metapth146056/. Accessed February 11, 2016.