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Article: Evidence of the Direct Involvement of the Substrate TCP Radical in Functional Switching from Oxyferrous O-2 Carrier to Ferric Peroxidase in the Dual-Function Hemoglobin/Dehaloperoxidase from Amphitrite ornata[Show abstract] [Hide abstract]
ABSTRACT: The coelomic O2-binding hemoglobin dehaloperoxidase (DHP) from the sea worm Amphitrite ornata is a dual function heme protein that also possesses a peroxidase activity. Two different starting oxidation states are required for reversible O2 binding (ferrous) and peroxidase (ferric) activity bringing into question how DHP manages the two functions. In our previous study, the co-presence of substrate 2,4,6-trichorophenol (TCP) and H2O2 was found to be essential for the conversion of oxy-DHP to enzymatically active ferric DHP. Based on that study, a functional switching mechanism involving substrate radicals (TCP•) was proposed. To further support this mechanism, herein we report details of our investigations into the H2O2-mediated conversion of oxy-DHP to the ferric or ferryl ([TCP] < [H2O2]) state triggered by both biologically relevant [TCP, 4-bromophenol (4-BP)] and non-relevant (ferrocyanide) compounds. When [H2O2] < 50 μM, all of these conversion reactions are completely inhibited by ferric heme ligands (KCN, imidazole), indicating an involvement of ferric DHP. Furthermore, the spin-trapping reagent 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) effectively inhibits the TCP/4-BP (but not ferrocyanide)-triggered oxy- to ferric DHP conversion. These results and [O2]-dependent conversion rates observed in this study demonstrate that substrate TCP triggers the conversion of oxy-DHP into a peroxidase by TCP• oxidation of the deoxyferrous state. TCP• is cumulatively generated, with increasingly produced ferric DHP, upon H2O2-oxidation of TCP catalyzed initially by trace amounts of ferric DHP present in the oxy-DHP sample. The data presented herein further address the mechanism of how the halophenolic substrate triggers the conversion of hemoglobin DHP into a peroxidase.
Article: Influence of Heme Environment Structure on Dioxygen Affinity for the Dual Function Amphitrite ornata Hemoglobin/Dehaloperoxidase. Insights into the Evolutional Structure-Function Adaptations.[Show abstract] [Hide abstract]
ABSTRACT: Sea worm, Amphitrite ornata, has evolved its globin (an O2 carrier) also to serves as a dehaloperoxidase (DHP) to detoxify haloaromatic pollutants generated by competing species. A previous mutagenesis study by our groups on both DHP and sperm whale myoglobin (SW Mb) revealed some structural factors that influence the dehaloperoxidase activities (significantly lower for Mb) of both proteins. Using an isocyanide/O2 partition constant measurement method in this study, we have examined the effects of these structural factors on the O2 equilibrium constants (KO2) of DHP, SW Mb, and their mutants. A clear trend of decreasing O2 affinity and increasing catalytic activity along with the increase in the distal His N(ε2)-heme iron distance is observed. An H93K/T95H Mb double mutant mimicking the DHP proximal His positioning exhibited markedly enhanced O2 affinity, confirming the essential effect of proximal His rotation on the globin function of DHP. For DHP, the L100F, T56G and M86E variants showed the effects of distal volume, distal His flexibility and proximal electronic push, respectively, on the O2 affinity. This study provides insights into how DHP has evolved its heme environment to gain significantly enhanced peroxidase capability without compromising its primary function as an O2 carrier.
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ABSTRACT: It is generally accepted that the inactive P420 form of cytochrome P450 (CYP) involves the protonation of the native cysteine thiolate to form a neutral thiol heme ligand. On the other hand, it has also been suggested that recruitment of a histidine to replace the native cysteine thiolate ligand might underlie the P450→P420 transition. Here we discuss resonance Raman investigations of the H93G myoglobin (Mb) mutant in the presence of tetrahydrothiophene (THT) or cyclopentathiol (CPSH), and on pressure-induced cytochrome P420cam (CYP101), that show a histidine becomes the heme ligand upon CO binding. The Raman mode near 220 cm-1, normally associated with the Fe-histidine vibration in heme proteins, is not observed in either reduced P420cam or the reduced H93G Mb samples, indicating that histidine is not the ligand in the reduced state. The absence of a mode near 220 cm-1 is also inconsistent with a generalization of the suggestion that the 221 cm-1 Raman mode, observed in the P420-CO photoproduct of inducible nitric oxide synthase (iNOS), arises from a thiol-bound ferrous heme. This leads us to assign the 218 cm-1 mode observed in the 10 ns P420cam-CO photoproduct Raman spectrum to a Fe-histidine vibration, in analogy to many other histidine bound heme systems. Additionally, the inverse correlation plots of the νFe-His and νCO frequencies for the CO adducts of P420cam and the H93G analogs provide supporting evidence that histidine is the heme ligand in the P420-CO bound state. We conclude that, when CO binds to the ferrous P420 state, a histidine ligand is recruited as the heme ligand. The common existence of a HXC-Fe motif in many CYP systems allows the C→H ligand switch to occur with only minor conformational changes. One suggested conformation of P420-CO involves the addition of another turn in the proximal L helix so that, when the protonated Cys ligand is dissociated from the heme, it can become part of the helix and the heme is ligated by the His residue from the adjoining loop region. In other systems, such as iNOS and CYP3A4 (where the HXC-Fe motif is not found) a somewhat larger conformational change would be necessary to recuit a nearby histidine.
Article: Characterization of Heme Ligation Properties of Rv0203, a Secreted Heme Binding Protein Involved in Mycobacterium tuberculosis Heme Uptake[Show abstract] [Hide abstract]
ABSTRACT: The secreted Mycobacterium tuberculosis (Mtb) heme binding protein Rv0203 has been shown to play a role in Mtb heme uptake. In this work, we use spectroscopic (absorption, electron paramagnetic resonance, and magnetic circular dichrosim) methods to further characterize the heme coordination environments of His-tagged and native protein forms, Rv0203-His and Rv0203-notag, respectively. Rv0203-His binds the heme molecule through bis-His coordination and is low-spin in both ferric and ferrous oxidation states. Rv0203-notag is high-spin in both oxidation states and shares spectroscopic similarity with pentacoordinate oxygen-ligated heme proteins. Mutagenesis experiments determined that residues Tyr59, His63, and His89 are required for Rv0203-notag to efficiently bind heme, reinforcing the hypothesis based on our previous structural and mutagenesis studies of Rv0203-His. While Tyr59, His63, and His89 are required for the binding of heme to Rv0203-notag, comparison of the absorption spectra of the Rv0203-notag mutants suggests the heme ligand may be the hydroxyl group of Tyr59, although an exogenous hydroxide cannot be ruled out. Additionally, we measured the heme affinities of Rv0203-His and Rv0203-notag using stopped flow techniques. The rates for binding of heme to Rv0203-His and Rv0203-notag are similar, 115 and 133 μM(-1) s(-1), respectively. However, the heme off rates differ quite dramatically, whereby Rv0203-His gives biphasic dissociation kinetics with fast and slow rates of 0.0019 and 0.0002 s(-1), respectively, and Rv0203-notag has a single off rate of 0.082 s(-1). The spectral and heme binding affinity differences between Rv0203-His and Rv0203-notag suggest that the His tag interferes with heme binding. Furthermore, these results imply that the His tag has the ability to stabilize heme binding as well as alter heme ligand coordination of Rv0203 by providing an unnatural histidine ligand. Moreover, the heme affinity of Rv0203-notag is comparable to that of other heme transport proteins, implying that Rv0203 may act as an extracellular heme transporter.
Article: Amphitrite ornata Dehaloperoxidase (DHP): Investigations of Structural Factors That Influence the Mechanism of Halophenol Dehalogenation Using "Peroxidase-like" Myoglobin Mutants and "Myoglobin-like" DHP Mutants[Show abstract] [Hide abstract]
ABSTRACT: Dehaloperoxidase (DHP), discovered in the marine terebellid polychaete Amphitrite ornata, is the first heme-containing globin with a peroxidase activity. The sequence and crystal structure of DHP argue that it evolved from an ancient O(2) transport and storage globin. Thus, DHP retains an oxygen carrier function but also has the ability to degrade halophenol toxicants in its living environment. Sperm whale myoglobin (Mb) in the ferric state has a peroxidase activity ∼10 times lower than that of DHP. The catalytic activity enhancement observed in DHP appears to have been generated mainly by subtle changes in the positions of the proximal and distal histidine residues that appeared during DHP evolution. Herein, we report investigations into the mechanism of action of DHP derived from examination of "peroxidase-like" Mb mutants and "Mb-like" DHP mutants. The dehalogenation ability of wild-type Mb is augmented in the peroxidase-like Mb mutants (F43H/H64L, G65T, and G65I Mb) but attenuated in the Mb-like T56G DHP variant. X-ray crystallographic data show that the distal His residues in G65T Mb and G65I are positioned ∼0.3 and ∼0.8 Å, respectively, farther from the heme iron compared to that in the wild-type protein. The H93K/T95H double mutant Mb with the proximal His shifted to the "DHP-like" position has an increased peroxidase activity. In addition, a better dehaloperoxidase (M86E DHP) was generated by introducing a negative charge near His89 to enhance the imidazolate character of the proximal His. Finally, only minimal differences in dehalogenation activities are seen among the exogenous ligand-free DHP, the acetate-bound DHP, and the distal site blocker L100F DHP mutant. Thus, we conclude that binding of halophenols in the internal binding site (i.e., distal cavity) is not essential for catalysis. This work provides a foundation for a new structure-function paradigm for peroxidases and for the molecular evolution of the dual-function enzyme DHP.
Article: The H93G Myoglobin Cavity Mutant as a Versatile Scaffold for Modeling Heme Iron Coordination Structures in Protein Active Sites and Their Characterization with Magnetic Circular Dichroism Spectroscopy.[Show abstract] [Hide abstract]
ABSTRACT: Preparation of heme model complexes is a challenging subject of long-standing interest for inorganic chemists. His93Gly sperm whale myoglobin (H93G Mb) has the proximal His replaced with the much smaller non-coordinating Gly. This leaves a cavity on the proximal side of the heme into which a wide variety of exogenous ligands can be delivered. The end result is a remarkably versatile scaffold for the preparation of model heme adducts to mimic the heme iron coordination structure of native heme proteins. In this review, we first summarize the quantitative evidence for differential ligand binding affinities of the proximal and distal pockets of the H93G Mb cavity mutant that facilitates the preparation of mixed-ligand derivatives. Then we review our use of magnetic circular dichroism and electronic absorption spectroscopy to characterize nitrogen-, oxygen-, and sulfur-donor-ligated H93G Mb adducts with an emphasis on species not easily prepared by other heme model system approaches and those that serve as spectroscopic models for native heme proteins.
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ABSTRACT: Cytochrome P450-mediated monooxygenation generally proceeds via a reactive ferryl intermediate coupled to a ligand radical [Fe(IV)═O]+• termed Compound I (Cpd I). The proximal cysteine thiolate ligand is a critical determinant of the spectral and catalytic properties of P450 enzymes. To explore the effect of an increased level of donation of electrons by the proximal ligand in the P450 catalytic cycle, we recently reported successful incorporation of SeCys into the active site of CYP119, a thermophilic cytochrome P450. Here we report relevant physical properties of SeCYP119 and a detailed analysis of the reaction of SeCYP119 with m-chloroperbenzoic acid. Our results indicate that the selenolate anion reduces rather than stabilizes Cpd I and also protects the heme from oxidative destruction, leading to the generation of a new stable species with an absorbance maximum at 406 nm. This stable intermediate can be returned to the normal ferric state by reducing agents and thiols, in agreement with oxidative modification of the selenolate ligand itself. Thus, in the seleno protein, the oxidative damage shifts from the heme to the proximal ligand, presumably because (a) an increased level of donation of electrons more efficiently quenches reactive species such as Cpd I and (b) the protection of the thiolate ligand provided by the protein active site structure is insufficient to shield the more oxidizable selenolate ligand.
Article: Alkylamine-Ligated H93G Myoglobin Cavity Mutant: A Model System for Endogenous Lysine and Terminal Amine Ligation in Heme Proteins such as Nitrite Reductase and Cytochrome f[Show abstract] [Hide abstract]
ABSTRACT: His93Gly sperm whale myoglobin (H93G Mb) has the proximal histidine ligand removed to create a cavity for exogenous ligand binding, providing a remarkably versatile template for the preparation of model heme complexes. The investigation of model heme adducts is an important way to probe the relationship between coordination structure and catalytic function in heme enzymes. In this study, we have successfully generated and spectroscopically characterized the H93G Mb cavity mutant ligated with less common alkylamine ligands (models for Lys or the amine group of N-terminal amino acids) in numerous heme iron states. All complexes have been characterized by electronic absorption and magnetic circular dichroism spectroscopy in comparison with data for parallel imidazole-ligated H93G heme iron moieties. This is the first systematic spectral study of models for alkylamine- or terminal amine-ligated heme centers in proteins. High-spin mono- and low-spin bis-amine-ligated ferrous and ferric H93G Mb adducts have been prepared together with mixed-ligand ferric heme complexes with alkylamine trans to nitrite or imidazole as heme coordination models for cytochrome c nitrite reductase or cytochrome f, respectively. Six-coordinate ferrous H93G Mb derivatives with CO, NO, and O(2) trans to the alkylamine have also been successfully formed, the latter for the first time. Finally, a novel high-valent ferryl species has been generated. The data in this study represent the first thorough investigation of the spectroscopic properties of alkylamine-ligated heme iron systems as models for naturally occurring heme proteins ligated by Lys or terminal amines.
Article: Probing the Oxyferrous and Catalytically Active Ferryl States of Amphitrite ornata Dehaloperoxidase by Cryoreduction and EPR/ENDOR Spectroscopy. Detection of Compound I[Show abstract] [Hide abstract]
ABSTRACT: Dehaloperoxidase (DHP) from Amphitrite ornata is a heme protein that can function both as a hemoglobin and as a peroxidase. This report describes the use of 77 K cryoreduction EPR/ENDOR techniques to study both functions of DHP. Cryoreduced oxyferrous [Fe(II)-O(2)] DHP exhibits two EPR signals characteristic of a peroxoferric [Fe(III)-O(2)(2-)] heme species, reflecting the presence of conformational substates in the oxyferrous precursor. (1)H ENDOR spectroscopy of the cryogenerated substates shows that H-bonding interactions between His N(ε)H and heme-bound O(2) in these conformers are similar to those in the β-chain of oxyferrous hemoglobin A (HbA) and oxyferrous myoglobin, respectively. Decay of cryogenerated peroxoferric heme DHP intermediates upon annealing at temperatures above 180 K is accompanied by the appearance of a new paramagnetic species with an axial EPR signal with g(⊥) = 3.75 and g(∥) = 1.96, characteristic of an S = 3/2 spin state. This species is assigned to Compound I (Cpd I), in which a porphyrin π-cation radical is ferromagnetically coupled with an S = 1 ferryl [Fe(IV)═O] ion. This species was also trapped by rapid freeze-quench of the ambient-temperature reaction mixture of ferric [Fe(III)] DHP and H(2)O(2). However, in the latter case Cpd I is reduced very rapidly by a nearby tyrosine to form Cpd ES [(Fe(IV)═O)(porphyrin)/Tyr(•)]. Addition of the substrate analogue 2,4,6-trifluorophenol (F(3)PhOH) suppresses formation of the Cpd I intermediate during annealing of cryoreduced oxyferrous DHP at 190 K but has no effect on the spectroscopic properties of the remaining cryoreduced oxyferrous DHP intermediates and kinetics of their decay. These observations indicate that substrate (i) binds to oxyferrous DHP outside of the distal pocket and (ii) can reduce Cpd I to Cpd II [Fe(IV)═O]. These assumptions are also supported by the observation that F(3)PhOH has only a small effect on the EPR properties of radiolytically cryooxidized and cryoreduced ferrous [Fe(II)] DHP. EPR spectra of cryoreduced ferrous DHP disclose the multiconformational nature of the ferrous DHP precursor. The observation and characterization of Cpds I, II, and ES in the absence and in the presence of F(3)PhOH provides definitive evidence of a mechanism involving consecutive one-electron steps and clarifies the role of all intermediates formed during turnover.
Article: Functional Switching of Amphitrite ornata Dehaloperoxidase from O-2-Binding Globin to Peroxidase Enzyme Facilitated by Halophenol Substrate and H2O2[Show abstract] [Hide abstract]
ABSTRACT: Amphitrite ornata dehaloperoxidase (DHP) is the first heme-containing globin possessing a native peroxidase enzymatic activity. DHP catalyzes the H(2)O(2)-dependent dehalogenation of halophenols. By possessing this detoxifying enzymatic activity, these organisms are able to thrive in an environment contaminated with toxic haloaromatics. It has been proposed that DHP evolved from a dioxygen carrier globin protein and therefore possesses dual physiological roles of O(2) carrier and dehaloperoxidase. Although DHP is isolated in the catalytically inactive oxyferrous state (oxy-DHP), we find that the combination of H(2)O(2) and the substrate 2,4,6-trichlorophenol (TCP) brings about facile switching of oxy-DHP to the enzymatically active ferric state via a process likely involving substrate radicals (TCP*). In contrast, in the absence of TCP, H(2)O(2) alone converts oxy-DHP to an inactive state (compound RH) instead of oxidizing the enzyme to the ferric state. Further, although the rate of autoxidation of oxy-DHP is somewhat enhanced by the presence of TCP, the effect is too small to be the functional switch. Instead, both substrate and H(2)O(2) are needed to convert oxy-DHP to the catalytically active ferric state. These observations provide a physiological link between the O(2) carrier role of the ferrous protein and the peroxidase activity of the ferric enzyme in this bifunctional protein.
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ABSTRACT: The mammalian circadian clock synchronizes physical and metabolic activity with the diurnal cycle through a transcriptional-posttranslational feedback loop. An additional feedback mechanism regulating clock timing has been proposed to involve oscillation in heme availability. Period 2 (PER2), an integral component in the negative feedback loop that establishes circadian rhythms in mammals, has been identified as a heme-binding protein. However, the majority of evidence for heme binding is based upon in vitro heme-binding assays. We sought to ascertain if these largely spectral assays could distinguish between specific and nonspecific heme interactions. Heme-binding properties by a number of other well-characterized proteins, all with no known biological role involving heme interaction, corresponded to those displayed by PER2. Site-directed mutants of putative heme-binding residues identified by MCD were unable to locate a specific heme-binding site on PER2. Protein film electrochemistry also indicates that heme binds PER2 nonspecifically on the protein surface. Our results establish the inability of qualitative in vitro assays to easily distinguish between specific and nonspecific heme binding. We conclude that heme binding to PER2 is likely to be nonspecific and does not involve the hydrophobic pocket within the PER2 PAS domains that in other PAS proteins commonly recognizes cofactors. These findings also question the significance of in vivo studies that implicate heme interactions with the clock proteins PER2 and nPAS2 in biological function.
Article: The proximal and distal pockets of the H93G myoglobin cavity mutant bind identical ligands with different affinities: Quantitative analysis of imidazole and pyridine binding[Show abstract] [Hide abstract]
ABSTRACT: His93Gly sperm whale myoglobin (H93G Mb) has the proximal histidine ligand removed to create a cavity for exogenous ligand binding, making it a versatile template for the preparation of model heme complexes. In this study, we have measured the first and second ligand binding affinities of imidazole and pyridine to form mono- and bis-ligated ferric and ferrous H93G Mb complexes. Electronic absorption spectroscopy has been utilized to determine the binding affinities for the proximal (Kd1, first ligand) and distal (Kd2, second ligand) pockets of H93G Mb. Magnetic circular dichroism spectroscopy has been used to confirm the identity of the complexes. The binding affinities for the first ligand are one hundred- to one thousand-fold higher than those for the second ligand (Kd1 « Kd2) for the same exogenous ligand. This is entirely opposite to what is seen with free heme in organic solvents where Kd1 » Kd2. Thus, the proximal pocket is the high affinity binding site. The lower affinity for the distal pocket can be attributed to steric hindrance from the distal histidine. This report provides quantitative evidence for differential ligand binding affinities of the proximal and distal pockets of H93G Mb, a unique property that facilitates generation of heme iron derivatives not easily prepared with other heme model systems.
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University of South Carolina
Columbia, South Carolina, United States
- Department of Chemistry and Biochemistry


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Great little take out place in the middle of Palmyra. Order here all the time and the food has always been goodread full review »
Great Taste Chinese Food is located at 111 E Broad St, Palmyra, NJ. This business specializes in Restaurants and has 3 review(s) with a star rating of 3.0.
Great Taste Chinese Food can be found at E Broad St 111. The following is offered: Restaurants. The entry is present with us since Sep 9, 2010 and was last updated on Nov 14, 2013. In Palmyra there are 4 other Restaurants. An overview can be found here.

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2 Corinthians 11:12New International Version (NIV)
12 And I will keep on doing what I am doing in order to cut the ground from under those who want an opportunity to be considered equal with us in the things they boast about.
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Synonyms of trader in English:
a market trader
dealer, merchant, buyer, seller, salesman, saleswoman, buyer and seller, marketeer, merchandiser, broker, agent;
shopkeeper, retailer, wholesaler;
British stockist
informal runner, pusher
dated pedlar, hawker
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Between train station and sights of Lecce and host very helpful and professional. Provided a nice map and great suggestions about where to purchase food and other places to go. Rooms were spotlessly clean. Happy to let us leave our bags and pick up later on the day of checkout. Do keep in mind this is a B&B so you... More
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Speed Drawing Competition (TrotCon 2012) by wildtielWatch
©2012-2016 wildtiel
This is just a page of randomness from the Speed Drawing competition at TrotCon 2012. I didn't win any prizes, but I had a blast regardless!
Art © Chris Behrendt
Speedily Drawn on July 22, 2012
Art © Chris Behrendt
Speedily Drawn on July 22, 2012
Hey sweet. Glad you enjoyed the Impromptu hosts you had!
Hahaha! Oh that's right! I completely forgot that the real hosts of that panel were no shows. Seriously, I loved that competition. Hope there is one at Trotcon this year!
I didn't even try :V I take an hour to draw decently LMAO
lol the scootaloo cutie mark is hilarious. I swear if that was her cutie mark she'd go crazy.
Lyra, you silly filly XD


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Analysis: Grimace
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Eclectic Institute, Mycetobotanicals, Cordyceps, 2 fl oz (60 ml)
By Eclectic Institute12 Reviews
Expiration Date:?May 2020
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Our receiving department does its best to verify and then enter the correct expiration dates for all incoming products. However, discrepancies do occur from time to time. This being said, the exceptionally high turnover at iHerb ensures that our inventory is among the freshest in the industry.
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Product Overview
- Fresh Mycelia
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15-30 drops 1-2 times per day, 30 drops represent approximately 1 gram of mycelia.
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Cordyceps (Cordyceps sinensis) fresh mycelia - Manufacturing strength 1:1 (Fresh weight to volume)
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iHerb Customer Reviews
About one dropperful of this, combined with 30-50 mg Germanium, is a good boost for stamina and oxygen use. I use it for exercise and to help my body cope with sleep deprivation. Takes about 30 minutes to be effective - good for hiking and other aerobic exercise. I take it as I get ready to sleep if I know I'm going to bed way too late, or when I get up if my sleep is cut short. Also good as a more general tonic if taken daily.
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He's a soon-to-be award-winning music video director extraordinaire - Director of Fun's "Why Am I The One", Grouplove's "Tongue Tied", and Meg Myers' brand new "Desire" vid (check it out!), JORDAN BAHAT came on and discussed shooting his first booty vid, why his mom gave him a (maybe?) loaded gun at the age of 15, and how a tan penis holds new meaning in his life after a trip to Spain. Enjoy!
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i co-host a cool podcast with my friends at twb.cool


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21 Brooklyn Beauties Give Us This Week's Hairstyle Inspiration view
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Naturalistas converged at the Design Essentials booth at the World Natural Hair Show. Be inspired by these curly cuties!
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Michele Harris stops by the Design Essentials booth at the World Natural Hair Health & Beauty Show in College Park, GA.
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Rory Mcilroy: 2012 in pictures
October 21, 2012
A member of the media holds a bobblehead of golfer Rory McIlroy before a baseball game between the San Francisco Giants and the Houston Astros in San Francisco, Tuesday, June 12, 2012. McIlroy was scheduled to throw out the ceremonial first pitch. The bobbleheads were given out to fans.
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I am graphic designer with more than eight years of professional experience. I have excellent skills, through knowledge of programs from Adobe Creative Suite including Photoshop, Illustrator and InDesign. I'm creative, hard-working and reliable graphic designer. I love challenges and I always strive to find a solution for every problem.
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text: Corrupt FDA Approves CLONED MEAT, MILK, CHEESE AND OTHER DAIRY Posted by UFOHUNTERORGUK on June 23, 2011 Posted in: GLOBALIST-NWO-NEOCON AGENDA. Tagged: 2012, 21, abduction, agenda, air, alien, aliens, appeaser, ASTRA, bilderberg, BILL, BLACK-OPS, britain, CLONED MEAT, close encounter, CODENAME, conspiracy, cooper, cover, coverup, disaster, ELENIN, et, EU, extraterrestrial, fema, flying, force, geo engineering, geocom, GOVT, haarp, history, illuminati, ISLAM, islamification, Jew, jidl, law, lies, media, modification, moon, morgellons, mosque, mossad, mothership, MUSLIM, NASA: Never A Straight Answer, new, NIBIRU, NWO, OBAMA, order, police, saucer, shapeshifting, superdarn, SUPERSTATE, T3RB, truth, truthers, tyche, ufo, up, uprising, weather, william, world, ZION, ZIONIST, ZOG. Leave a comment Uploaded by MaryGreeley on 22 Jun 2011 Share this:TweetEmailPrintShare on TumblrPocketLike this:Like Loading... Related Posts navigation ← Fox News Tries To Bribe Reporters : Media Blackout – FDA Approves Monsanto Poison Milk. 6.7M & 6.8M Rocks Japan. Tsunami Alert Issued →


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Mathematics & Statistics (Sci) : Taylor series, Taylor's theorem in one and several variables. Review of vector geometry. Partial differentiation, directional derivative. Extreme of functions of 2 or 3 variables. Parametric curves and arc length. Polar and spherical coordinates. Multiple integrals.
Terms: Fall 2010, Winter 2011, Summer 2011
Instructors: Wilbur Jonsson, Neville G F Sancho (Fall) Wilbur Jonsson (Winter)


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Guayaki, Organic Yerba Mate, 25 Tea Bags, 2.6 oz (75 g) (Discontinued Item)
By Guayaki102 Reviews
Shipping Weight:?0.64 lbs (0.29 kg)
Shipping Weight
The Shipping Weight includes the product, protective packaging material and the actual shipping box. In addition, the Shipping Weight may be adjusted for the Dimensional Weight (e.g. length, width & height) of a package. It is important to note that certain types of products (e.g. glass containers, liquids, fragile, refrigerated or ice packed) will often require protective packaging material. As such, these products will reflect a higher Shipping Weight compared to the unprotected product.
- Product Code: GSR-96201
- UPC Code: 632432962016
- Package Quantity: 25 Tea Bags, 2.6 oz (75 g)
- Dimensions: 3.2 x 3.4 x 6 in, 0.3 lbs (0.14 kg)
Product Overview
- Vitality · Clarity · Well-Being
- Organic
- 25 Traditional Yerba Mate Tea Bags
- Naturally Caffeinated
- Dietary Supplement
- Rich, Robust, and Balanced
- Fair for Life
- Social & Fair Trade
- Certified by IMO
- Certified Fair Trade
- USDA Organic
- Gluten-Free
- Kosher
- Fair Trade Federation Member
- Certified Organic by CCOF
- Certified Corporation
- Non GMO Project Verified
Traditional Yerba Mate
Guayakí (gwy-uh-kee) Yerba mate (yer-bah mah-tay) is made from the leaves of the celebrated South American rainforest holly tree (ilex paraguariensis).
Of the six commonly used stimulants in the world, (yerba mate, coffee, tea, kola nut, cocoa and guarana,) yerba mate triumphs as the most balanced, delivering both energy and nutrition.
For centuries, South America's Aché Guayakí tribe have sipped yerba mate daily for its powerful rejuvenative effects. These rainforest people find tremendous invigoration, focus and nourishment in yerba mate and revere it as the "drink of the gods".
- Helps stimulates focus & clarity
- Boosts physical energy
- Aids Elimination
- Contains antioxidants
- Traditionally used to support weight loss program that include a balanced diet and exercise
Suggested Use
How to Prepare
|Hot Mate
|Mate Lattè
|Iced Mate
|Place tea bag in a cup of hot (not boiling) water. Steep 5-10 minutes.
|Steep the Mate, then add your favorite milk and sweetener.
|Steep hot, then add ice and lemon to taste.
To experience the full health benefis of Yerba Mate daily, we recommend steeping 2-4 tea bags.
Other Ingredients
100% organic, sustainably harvested, rainforest-grown yerba mate.
Each mate tea bag contains the caffeine equivalent of 1/3rd cup of coffee.
Do not drink mate above 150 degrees fahrenheit.
Store in a cool, dry place.
|Supplement Facts
|Serving Size: One tea bag (3 grams)
|Servings Per Container: 25
|Amount per serving
|%Daily Value*
|Calories from fat
|Total Carbohydrate
|1 g
|Organic Yerba Mate (leaf and stem)
|3 g
|*Daily Value based on a 2000 calorie diet
†Daily Value not established
iHerb Customer Reviews
I discovered Yerba Mate about ten years ago and drink it without fail every single morning. Personally, I don't know what I'd do without it!While coffee can ruin your system and make one nervous, Yerba Mate delivers a clarity, awakeness and balanced aliveness. Supposedly, it contains caffeine, but a nutritionist once told me that the compounds in Yerba Mate are "a cousin" of caffeine, but the FDA requires it to be called caffeine. If that's true, it would explain why it stimulates but IS NEVER HARSH on the nervous system or adrenals like coffee is. I think it's the PERFECT COFFEE SUBSTITUTE. I confess I drink more than a single bag - up to four bags a day sometimes. It is great for waking up, before meditation, before working out and super great for writing, reading and studying. While, Yerba Mate is fine by itself, I highly suggest trying a little chocolate flavored (or your favorite flavor) Almond Breeze, Soy Milk or Rice dream for flavoring and sweetness, or adding a little stevia. For us, Guayaki Yerba Mate tea is the most incredible tea on the planet!
What, this has something like 24 vitamins and minerals, and 15 amino acids...gives you energy, clarity and focus, and yes, ladies, can help with weight loss b/c can help curb your appetite...awesome stuff!! And good price on iherb!!


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Rico Rodriguez (born July 31, 1998) is an American actor. He is best known for playing Manny Delgado in the ABC sitcom Modern Family. He also wrote a book that was published in 2012.
List of links to Rico Rodriguez fansites:


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Categories: Handjob, Hardcore, Teens
Submited: 6 years old
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On Friday, April 15, the U.S. House of Representatives is scheduled to vote on Republican Congressman Paul Ryan's 2012 budget proposal, which seeks to shred the Pell Grant program. These grants have enabled millions of economically disadvantaged Americans to realize their dreams of a college education and brighten their prospects for the future.
Rep. Ryan's proposal wouldn't just skim a little off the top. Experts anticipate that it would reduce the maximum Pell Grant award by up to 60 percent – from $5,500 per year to as low as $2,100. And since colleges across the country are raising tuitions, such cuts would be catastrophic for many students. Consider this: In 1980, the maximum Pell grant covered most of the cost to attend a four-year public college. By 2007, it covered less than one third – an historic low.
Tell your congressional representative that these are cuts America can't afford. Don't delay!
Education Trust started this petition with a single signature, and now has 136 supporters. Start a petition today to change something you care about.
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Richard T. Jones, Jazsmin Lewis, Aaron D. Spears, Victor Williams, Mia Morrow, Marlon Young in Traci Townsend
Movies.com, the ultimate source for everything movies, is your destination for new movie trailers, reviews, photos, times, tickets + more! Stay in the know with the latest movie news and cast interviews at Movies.com.
Copyright © 2016 FANDANGO MOVIES.


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Obama press conference on surveillance starts at 3pm ET (wwws.whitehouse.gov)
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Smolensk Petycja! (wwws.whitehouse.gov)
submitted by pezGato to /r/poland
/r/politics [removed] Current Pledge Hypocrisy:Sign this petition not only reaffirming your rights but, Human Rights of all men;the rights of everyone including the very one that is included in the pledge; a renaissance of rebirth for America if passed, we can start out "Impartial" adhering only to (wwws.whitehouse.gov)
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Obama Administration response to NASA petition: not much of substance. (wwws.whitehouse.gov)
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Life Extension Research is not officially funded in the US - even though, apart from making us biologically immortal, it will also cure most existing terminal diseases & massively improve quality of life for billions of people. (wwws.whitehouse.gov)
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BAZIC 8-Digit Pocket Size Calculator w/ Flip Cover (Case of 144)Item#: BAD-102126086 | Model#: 3004-144
Currently Out Of Stock!
Product Details
Manufactured by: Bazic Products
Warranty provided by: Bazic Products
Limited Warranty: 1 months parts; 1 months labor
Mfg Part No: 3004-144
UPC No: 20764608030046
Box Size: ( Length:22, Width:15, Depth:11)
Shipping Weight: 24.5000 pound(s)
Click here for full warranty and support information
- Limited Warranty: A full text version of the limited warranty
may be obtained by mailing a self addressed, stamped envelope to the
address below and requesting the warranty for item number: BAD-102126086
Warranty Information
7795 W. Flagler St. Suite 35
Miami, FL. 33144
- Bazic Products logos are registered trademarks of Bazic Products. All others trademarks and copyrights mentioned herein are the property of their respective owners.


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April 29, 2014
Jason Howell
Rivals.com Football Recruiting
Joseph Prevost has picked up a few offers over the last few months. On Monday he received his latest and this one had a unique family tie. ...More... To continue reading this article you must be a member. Sign Up Now!
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text:
If difficulty in use is to be blamed on the designer, and interfaces get better over time, the challenges that humans face in society will not as often require us to use our analysis and problem-solving skills. Of course there are higher-level problems such as poverty, disease, the environment -- the list goes on and on -- but it is the lower-level problems that we encounter every day that train our brain and our consciousness to engage in, and succeed at synthesis and solutions. Have society and the human condition gotten better from making the world easier to navigate? I'm not sure about this one, but the cranky old men of the world sure don't think so.
The Slow Death of Nuance
If difficulty in comprehension is to be blamed on the writer, then writers, bloggers and content strategists will push themselves to get more simple in their use of written words. In the same way that good user experiences and interfaces render complex series of actions simple through a visual interface (or through an awesome API for the API geeks in the house), writers will then be pushed to bury nuance and distinction in simpler language to be accessible to the masses. As articles, blogs and other written forms are driven to accessibility, will public discourse be far behind? I would argue that public discourse has actually been in the lead, given the ideological narratives, espoused by both sides, that abound on television and radio.
As we continue to bury nuance and distinction, I fear we will lose the ability to describe, see or understand the distinction between different ideas, concepts and artifacts. Many of the great philosophers and writers throughout history have claimed that the ability to identify, understand and communicate distinction is the basis for all knowledge, progress and wisdom in society. The more that nuance and distinction are buried, the more we tread down the road to anosognosia -- a condition in which a person who suffers from a disability seems unaware of or denies the existence of their disability.
The Silver Lining
What will take us off this path to destruction? Where will people find challenges that hone their problem-solving skills, raise their awareness of nuance and distinction? A recent outcome of the UX movement that many people are familiar with is gamification. Many UX practitioners have just discovered the value that games and game mechanics can add to their products and and services and the application of these principles and ideas vary widely (articulated quite fabulously by the ever-brilliant Kathy Sierra).
I do believe that the rising popularity of games will help people in developing their analysis and problem-solving skills and I have to hope that some brilliant game designer with a passion for language will eventually turn their head toward making a game that helps people develop their skills for identifying and communicating distinction. If only it were as simple as "Draw Something!" Or maybe it is. Anyone up for developing "Write Something!"?


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I am on H1B visa in US. I came to california,US on Dec 2013. I have filled the tax through turbo tax e-file. When I checked it is showing 1040EZ for fedral and 8453-OL for state. I am not sure if it is correct. Please tell what should be correct form for me. What should I do now?
This is my stay break up in USA
20 Sep 2012 to 14 Dec 2012 -- makes this 29 days in 2012
and 12 Dec 2013 to till date --- makes this 20 days in 2013
hence i have neither of the below conditions
31 days during the current year, and
183 days during the 3-year period that includes the current year and the 2 years immediately before that,
Nor have i passed the green card test .
So i am a Non Resident alien and should have filled Form 1040NR or 1040NR-EZ.
but for me the form is 1040EZ for fedral which is for Resident Aliens. And is incorrect.
So please tell me if.
1. This has no impact
2. Or that i need to fill up another form
3. Or can this be taken care of from Turbo tax side.
Thanks and Regards
Deepika Kapoor
Unfortunately, TurboTax does not support the 1040NR. So if you need to fill out the form 1040NR you will not be able to fill this form out with TurboTax.


text:
Daniel 5:3New King James Version (NKJV)
3 Then they brought the gold vessels that had been taken from the temple of the house of God which had been in Jerusalem; and the king and his lords, his wives, and his concubines drank from them.
New King James Version (NKJV)
Scripture taken from the New King James Version®. Copyright © 1982 by Thomas Nelson. Used by permission. All rights reserved.
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text:
Michael Basil Caroline Schooler Byron Reeves Stanford University
Political candidates attach increasing importance to negative television advertising. In recent campaigns, the number of negative ads has increased dramatically ( Merritt, 1984). During the 1988 presidential campaign, the popular press asserted that negative political advertising had reached an all-time high ( McLeod, 1989; Shapiro & White, 1988; Stengel, 1988; Taylor & Broder, 1988). The press also felt that Dukakis' failure to respond to Bush's attacks in the 1988 presidential campaign affected the election ( Beatty, 1988; Colford, 1988). That is, negative ads appeared to tip the balance in the election.
The increase in negative ads is partially attributable to proprietary research that shows negative messages have powerful effects ( Sabato, 1981). Candidates hope that attacking ads will lead voters to dislike or fear opponents, and consequently, negative ads are seen as more damaging to the attacked candidate than the attacker ( Kaid & Boydston, 1987). This may be a good political strategy because feelings about candidates have been shown to be important determinants of voting decisions ( Abelson, Kinder, Peters, & Fiske, 1982; Brody & Page, 1973; Foti & Lord, 1982; Lau, 1986; Markus & Converse, 1979).
An important factor in political advertising is the context or sequencing of advertisements. Two important decisions that political strategists face are whether to initiate attacks, and whether to respond to attacks from other candidates. Another context effect is whether running a collection of negative ads provides a cumulative advantage or a backlash against the candidate who sponsors them.


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Taryn flashes to show whats up her skirt.
more of these! PLEASE!!
nice, pose pretty model, scene, just caught my eye, nice thanks
Dear god! Wait, how old is she?
whats her full name
Great POV and slight CT from the panties. Perfect!
Very nice
aw sweet
Nice flash! Such innocent naughtiness!
Thanks for the show, Taryn! You're awesome!
Shes one naughty girl
She is such a pretty tease.
What i wouldn't give to be there! ! !
Cute peek!
anyone hungry?? I am...
wow..what a shot. Wonder what she's UP to???
I like your sexy panties.
Love it !
See, Mr. Principal, I'm not wearing thongs like you said I was.
Wow nice


text:
So with the Democrats divided and in disarray on Capitol Hill, and a significantly weakened White House sending confused and mixed messages about where it is headed next, GOP leaders decided this was not the best time for a meeting and cozy upstairs dinner with the president and Democratic leaders.
Obama needs this meeting more than they do. He was the one who promised to change the partisan tone in Washington, then led a sharply partisan attack on the Republicans in the final weeks of the midterm elections.
His economic stimulus schemes have been colossal and costly failures, and he and his party have run out of ideas about what to do next. Economic forecasters at the Fed and elsewhere see slower growth next year and beyond, and still the White House is calling for higher taxes on an anemic, jobless economy.
His trade trip to Asia, immediately after his policies and his party suffered a massive rebuke from the voters, was seen as a complete failure. He has refused to submit three negotiated trade agreements for ratification and done little to open up new markets for U.S. exports because of deep opposition from organized labor.
The White House suffered a further setback this week after Sen. Jon Kyl of Arizona, the second-ranking Republican, said there was no time to consider the new START nuclear arms treaty in this lame-duck session. Obama needs 14 Republicans to reach the 67 votes for approval, and Kyl's decision gives the GOP the 14 that they needed to postpone action until next year.
So, this lame-duck session that made a mountain of unfinished budget bills is shaping up as an uncomfortable experience for Obama and his party. The biggest battle will be whether to extend all of the Bush tax cuts, as Republicans want, or raise the two top tax rates on businesses and employers while the U.S. economy is still struggling to lift itself out of this recession.
This will be Topic A between Obama and the Republicans when they meet on Nov. 30. The betting in this corner is that Obama will seek some kind of a temporary extension on the grounds that this is no time to be raising income taxes on anyone. But right now the House Republicans want a permanent extension.
Donald Lambro is chief political correspondent for The Washington Times.
Rush Limbaugh defends Rubio's debate answer: He's absolutely right that Obama knows what he's doing | Allahpundit
Time for Team Hillary to break out the gender card for young liberal women who prefer Sanders | Allahpundit


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Application to the Psychology Graduate Program:
Select Application link and complete the Graduate Application, then complete the Supplemental Application posted below. (Both are required.)
General Experimental Letter of Recommendation Form:
Three letters of recommendation are needed. Send completed forms to the Graduate Assistant: Jessica Worland.
University Graduate Admission Application Forms:
Admissions and Records Student Forms includes change of objective, diploma application, graduation date change, change of address, name change, and more.
Apply for your graduate diploma in Psychology:
Application for a Masters Degree and Diploma
Additional Graduate Studies Information and Forms
Graduate Studies Information includes graduate degree information and evaluation services.
Download a copy of the Guidelines for the Preparation of Theses and Graduate Projects.
Option Chairs
General Experimental Psychology: Dr. Andrew Ainsworth and Dr. Mark Otten


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Seriously....I can not believe this didn't get more attention. I mean, yeah, cv is nothing new, but seriously!!!!!!! this is an amazing plugin. one I intend to buy. I mean, i can't believe anyone who has a analog modular, or pre midi synth isn't going coo coo for coa coa puffs over this. at last, someone has taken the obvious and made it brilliant!! good job MOTU!!!
22-Jan-09 01:54 AM
From soft and subtle to insanely brutal this osc can provide the lot


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Urban Decay 24/7 Glide On Eye Pencil in Stash
153 reviews
88% would repurchase
Package Quality: 4.2
Price: $$$
Package Quality: 4.2
Price: $$$
Not tested on animals
Urban Decay is a Cruelty-Free Subsidiary.
INGREDIENTSCyclopentasiloxane, C11-12 Isoparaffin, Paraffin, Polybutene, Simmondsia Chinensis (Jojoba) Seed Oil, Hydrogenated Cottonseed Oil, Ceresin, Ozokerite, Cera Microcristallina (Microcrystalline Wax), Tocopherol, Phenoxyethanol, Ascorbyl Palmitate
Age: 25-29
Skin: Combination, Other, Not Sure
Hair: Other, Other, Other
Eyes: Other
this is only eyeliner that looks good on me and it is the only one i wear. people with dark brown eyes, you NEED this eyeliner! i have fair skin with dark brown eyes and hair, i cannot wear black eyeliner because its too harsh. but i swear this color makes my brown eyes glow. without stash, my eyes just look plain 'dark', but once i apply it, my eyes turn into a beautiful shade of warm chocolate brown.
like others have said, stash is an olive green with just enough shimmer and no giant specks of glitter. it is so soft and easy to apply for make-up beginners such as my self. suitable for day or evenings because it does not look harsh or too dark once applied. i have oily lids and this does smudge a little, but a little primer keeps it locked into place for 12 hours. i have such love for stash. one of the few makeup items i've actually repurchased over and over. if this color is ever discontinued i will cry. there's nothing else like it.
Age: 18 & Under
Skin: Combination, Medium, Not Sure
Hair: Black, Other, Other
Eyes: Brown
I'm really mad at myself for using this eyeliner for so long. I'm sorry to be the downer here, but it really isn't very long lasting. It does feel really nice on my skin, and doesn't irritate it as much. It also admittedly looks nice if you're just going out for a bit. The thing is, the lead breaks off so easily, the makeup doesn't last long enough, and the pencil itself sharpens away in no time.
Age: 30-35
Skin: Other, Other, Not Sure
Hair: Other, Other, Other
Eyes: Other
Urban Decay 24/7 liners are the BEST!! I LOVE this product because it stays on yet will blend easily, doesn't transfer to my oily upper eyelid, and comes in fabulous colors. I really do love the Stash color. It's a nice change from basic black or brown. The metallic is great for everyday--not as dramatic as glitter. Easy to apply and seems to come off pretty easily with a basic facial cleanser.
Age: 30-35
Skin: Normal, Tan, Warm
Hair: Black, Straight, Fine
Eyes: Brown
I love this eyeliner. I've been using this as a base under my green and brown eyeshadows and the combinations are beautiful. I'm so glad I got this during the 30% off urban decay sale.
Age: 19-24
Skin: Oily, Fair-Medium, Not Sure
Hair: Blond, Other, Other
Eyes: Blue
Very nice! A great universal liner color that goes on smooth and lasts all day.
Age: 19-24
Skin: Normal, Fair, Not Sure
Hair: Brunette, Other, Other
Eyes: Brown
Colors works perfect for brown eyes
Age: 30-35
Skin: Combination, Medium, Not Sure
Hair: Brown, Other, Other
Eyes: Hazel
It is a great eyeliner. I also recommend this for hazel eyes. However I find this eyeliner a little heavy for daytime. But it is gorgeous for a nighttime look. Worth the price.
Age: 19-24
Skin: Normal, Fair, Neutral
Hair: Red, Wavy, Medium
Eyes: Hazel
this is my favorite eye liner!! i first off love urban decay e/ls anyways but when i saw this color, i fell in love...my eyes are hazel, so this e/l really brings out the green in my eyes and draws very good attention to them...i would recommend this e/l to anyone!! this color is really easy to put on when you need to give a little color to your eyes without over doing it...and of course, ud always stays on a long time for me
Age: 25-29
Skin: Combination, Fair, Not Sure
Hair: Red, Other, Other
Eyes: Blue
Love it. Most perfect eyeliner ever. Need I say more?
Age: 19-24
Skin: Combination, Fair, Not Sure
Hair: Blond, Wavy, Medium
Eyes: Green
This is a great eyeliner. The colour definitely doesn't look green when you put it on-more like a blackish-brown. I'm personally not a fan of the sparkles, but they aren't too noticeable either. I avoid the harshness of black liners, since I have pale skin and light hair, but this is softer and better for everyday use. Finally, great lasting power.
Popular Eyeliners
380 Reviews
658 Reviews
381 Reviews
2179 Reviews


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Will USC win a game in the Pac-12 Tournament?
It doesn't look good. I examine the sorry state of the Trojans in this preview of today's opening-round game against UCLA.
For coverage of pro day and the Pac-12 Tournament, be sure to check back here later today. And don't forget to follow me on Twitter: @LevOnUSC.
More USC basketball posts:
[rssfeedme cat="usc" feedpath="category/hoops/feed/" max="5"]
More from the USC blog:
[rssfeedme cat="usc" feedpath="category/football/feed/" max="5"]


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Insect Shield ® Hiker-style Sun Hat with Chin Cord
Introducing an extraordinary line of headwear that not only protects you from the sun but also repels biting insects. Insect Shield's active ingredient is Permethrin, a man-made form of a natural insect repellent, to provide protection against biting insects such as mosquitoes, ticks, flies and chiggers. These insects are known to carry dangerous diseases including West Nile virus and Lyme disease. Insect Shield products are EPA registered and offer convenient, comfortable and long-lasting insect protection through 70 washes. It creates a barrier while you work, reducing the need for messy sprays and oily repellents.
This Insect Shield hiker-style sun hat features side snaps allowing it to be worn in several different configurations. A large 3" brim provides ample protection from the glaring sun. Four large screened eyelets help to circulate air to keep you cool. Crown features a removable thin foam pad which keeps the hat afloat if dropped in the water. Adjustable chin cord keeps your hat secure in the windiest of conditions. Moisture-wicking sweatband. Imported. Specify color and size: M (7 to 7-1/8), L (7-1/4 to 7-3/8) or XL (7-1/2 to 7-5/8) from the selection box above. See drop down for colors and sizes currently available.
NOTE: Size XL in Tan color is seasonally out of stock until approximately March 2016.


text:
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Germantown playground fire
Police are asking for your help finding the person who set fire to a Germantown playground. The fire was set at the playground in the 21300 block of Seneca Crossing Drive next to Sally Ride Elementary, say Montgomery County Fire officials. The damage is estimated at more than $100,000, say fire officials.
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IV Therapy
To Be Announced
Cost: $249.00
This is a 16-hour class and clinical featuring an overview of Intravenous Therapy. Professionals providing IV therapy must continually keep current as IV Therapy continues to change. The purpose of the class will be to prepare health care professionals for introductory knowledge of Intravenous Therapy, including roles and responsibilities, and legal and ethical issues.
During this course, students will receive instruction on performing IV therapy with a focus on anatomy and physiology, asepsis, the equipment and supplies necessary, review of drug calculations, pharmacology as it related to IV therapy, and the practice of starting and running an IV. This class will involve hands-on opportunities with equipment and supplies, artificial and real IV Placement.
To register, contact:
Anita Fischer Administrative Associate Workforce Development, Corporate Training & Community Education
Work Phone: 304.424.8271work Work Email: email@example.com


text: The former home of Ferrazzano Florists will officially re-open Thursday as Advance Auto Parts. The store is at 582 Metacom Ave. (north of Fat Belly's). Carter Petter, the new general manager, said the nationwide retailer chose Metacom because it is close to customers and also nearby a host of garages where people take their cars for repair. The store may be new to Warren, but Advance has been around. The company currently operates 3,692 stores in 39 states, Puerto Rico and the Virgin Islands. Warren's store hours are Monday through Sunday, 7:30 a.m. to 9 p.m.


text:
Church Video Illustrations about Commitment
Media Collection of Church Videos, Sermon Video Illustration, Mini-Movies and Films
PRO member? Sign in and download now.
Not a PRO member? Start a free trial for immediate & unlimited download access.
Results: 36 Video Illustrations
Colorful, time lapsed chalk drawings interwine with bible verses to illustrate powerful song lyrics in this fast paced, upbeat video. The result is a passionate, prayerful declaration to seek God and put him above all other pursuits. The song More by Todd Vaters (CCLI Song #4575784) is available on iTunes.
Scripture: Psalms 63:1, James 4:8, 1 Chronicles 16:11
There are so many small parts to a mother's life that it can seem ordinary, but there is nothing ordinary about the role of "mother." This will connect with and empower every mom to remain strong. Text from a blog post by Lisa-Jo Baker who believes motherhood should come with its own superhero cape and blogs about it at lisajobaker.com.
Scripture: Exodus 20:12, Deuteronomy 5:16, Proverbs 31:10-31:31
Tags: Perseverance, Parenting, Comfort, Women, Commitment, [more]
Instead of focusing on human willpower for New Year resolutions, this video brings the focus back to God and his commitment to us. It's a great theme for a January sermon series and can even be shown later in the year when New Year's resolutions are long forgotten but God is still keeping his promises.
Scripture: Hebrews 13:5-13:6, Psalms 32:8, Jeremiah 29:11
Tags: New Year's Day, Goals, God's Faithfulness, God's Promises, Hope, [more]
The time to worship is now. The day to surrender is here. The moment to lay our lives down, as a sacrifice of praise, is upon us. This upbeat worship intro is a great way to call your congregation to worship and set the tone for your next Sunday service.
Scripture: Romans 12:1, Philippians 1:20-1:21, Romans 6:4
By: Beamer Films
Make the story of Hosea personal to your congregation. This video takes the principles from that story and applies them to a present day couple. This parable shows that God's love is unconditional, his grace unceasing, and his forgiveness readily available for each of us. This video was formerly titled "Wounded Bride."
Scripture: Hosea 3:1, Romans 6:8-6:18, Isaiah 55:1-55:5
Fall is here! Time to put away the barbecue, ditch the sunglasses and bust out the sweater collection. It's time to taxi the kids to school, practice, rehearsals and recitals. Fall can be exhausting. It's tempting to skip church just so we can catch a break. But, we serve a God who has never once taken a day off from loving us. We belong in community, we belong in accountability, we belong together as the body of Christ. This weekend, make church a priority. Hey, Fall is here…we'll see you at Church!
Scripture: Hebrews 10:25, Hebrews 13:17, Acts 2:42
How often do we stop and think about the worth of God? How often do we worship Him. How often do we sing His praises? The reality is, He never stops being worthy. He deserves to be honored and worshipped every moment of every day. How much is God worth to you?
Scripture: Psalms 68:4, Isaiah 25:1, Psalms 71:8
By: Shift Worship
"For my comrades who have fallen . . ." Colonel Lester L. Aerosmith, USAF, ret., urges us to join him by standing, remembering and renewing our commitment to freedom "for they have died to make it last."
Scripture: John 15:13
Tags: America, Patriotic, Memorial Day, Veterans Day, Holidays Civic, [more]
Worship is about living every moment of every day as a sacrifice of praise. It's about giving God the glory He deserves, no matter your circumstance. We were created to give praise to God and to live lives of worship.
Scripture: Psalms 150:6, John 4:24, Psalms 103:1
Tags: Surrender, Praise, Worship, Discipleship, Church Attendance, [more]
Life, at its very core, is about worship. It's about living out what we were created to do. It's about taking each moment of each day and laying it at the feet of Jesus as a sacrifice of praise. God has placed us here to worship.
Scripture: Psalms 150:6, John 4:24, Psalms 103:1
Tags: Prayer, Surrender, Worship, Praise, Commitment


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It was interesting to follow the tweets of supporters of various political parties during the last few weeks of election campaign. One of the most interesting, funny and somewhat arrogant tweets were of a Rock Star Salman Ahmed of Junoon band, who was campaigning for PTI.
There is nothing wrong to be enthusiastic and positive for the victory, but one should not cross the lines of decency and should not become self-appointed spokesperson for majority of Pakistani nation to save himself from embarrassment at later stage.
Here are some of selected tweets from Salman Ahmed:
36 Comments
we Pakistanis have given up isla,,so our culture is this ghaleez hindu culture like sulman khan.arrogant and kammena culture
PTI has taken the wrong route by giving key ministries to JI.
Not being on good terms with maulana could be icing on the cake. Cherry on the icing is giving up on the NA-1 seat.
So much to say for the political acumen.
I do recall IK preferring young blood over experience in all his campaign slogans. Maybe he didn't remember that while preferring khattak over asad qaiser.
Well, what can i say?
Its not the first time ive seen pti supporters cross their limits, like leader like followers.
Their leader called maulana fazlu munafiq and to worry about his akhirat on may 11th. The man who called ye balla circus ke sher ke 11 may ko phenti laga de ga, 'oye nawaz sharif', whose followers abuse and attack personally pml n supporters forget that these were the same brothers who postponed their jalsa, who got ijtimai dua done for his health, who forgave him after winning elections and even apologized for any mistake on their part and the same brothers who are giving him a chance in KPK while they could have teamed up with JI and formed government there.
Carry on, pti followers, and then you question why pti lost elections. Allah is Knows and Sees all.
I feel pity on administer. If discussion has to be generated, must be on noble man. Do this KANJAR is worth enough to be discussed. Shame on who created this thread!
Pakistan will never succeed until we start thinking like one nation. We have more hatred and contempt for our brothers than for our enemies. Elections are over and we are still wasting our time in either doing chamchay bazi or insulting others.
Many regulars on this forum want PTI to fail and ruin KP. It seems, they will get a lot of satisfaction if Allah forbid that ever happens and our people suffer more than they already have.
Clearly this forum is showing to the world Pakistan is a fragmented nation of very sick people.
Pasha Tyrian Investment (PTI) is disgracing Pakistan though their BURGER CLUB. They do not have evidence and making manufactured video based on previous elections, particularly by election. FAFEN was fixed today by ECP. See profile of this NGO, sponsors mean WEST sponsors are same who want technocrat Government in Pakistan, later invested on Abdul Shakoor Qadri (joker already fixed by SCP, Mubasher Luqman paid Malik Raiz employee is giving him life)and PTI to block entry of LION into Pakistan politics. I always bow head to Allah that He the kind saved us from evil change. Even PTI sister sponsored NGO FAFEN submitted apology to ECP, said out of 9000 polling stations they monitored irregularities were found in only 49, which comes to .06%. Mean election were fair by 99.94%. Other international media also agree with it including EU. They consider their effort to block LION failed as PTI BURGER CLUB is impotent. Look their CHEERLEADER is feeling ashamed to come in open, refuging in SKMH, whereas on poor people in fall are at their homes. Malik Riaz paid channels and anchors are also degrading Pakistan on direction of PTI key investor (one of among many) Malik Raiz. PTI is so impotent that did not have courage to accept Saad Rafique challenge and a clown who win after tempering in posh ladies polling stations Shafqat Loota, even said their is no fun in thumb impression, as ballot paper did not contain it. It will be hard to know, who is voted for by whom.
Losers are asking BAKSHISH on Lalak Chowk Lahore and Karachi using BURGER CLUB and SKMH employees. Kanwer Dilshad (master temper of election in 2008), who is Election Cell employee of PTI is giving comments on various channels as neutral expert. Government to take action, as if it is right of BURGERs to protest, it is also human right of commoner that his life is not disturbed.
Stop disgracing Pakistan and earn with respect. In BAKSHISH (charity) you even can not get burger from KFC/MacDonald. You sell your JUNOON at Bhatti gate Lahore and Boat Basin Karachi to earn it. So stop thinking for BAKSHISH seat in our Pakistan Parliament.
Can some one send this Kid @Hassan1995 to school. He need to grow up from the conspiracy mentality. Elections are over .
Now from Ethnic division, we have Burger club and poor/middle class classes.
MQM is also saying same that middle class is with them and Burger/educated class with PTI , i am trying to comprehend if KPK is Burger class as they voted PTI.
Even Saad Rafique on Hamid Mir sais teh samethat these English speaking Women of PTI etc..
m khan.its true that only burgers mummy daddys and secular youth are with pti.thats why its a fact that in kpk the pti,s mandate is fake,they were given majority their by agencies so kpk people also become secular and stop doing jihad against America….this is a fact and writing is on the wall!do fair elections in kpk today and pti wont win more than 5 seats I assure u,IT WAS AGENCIES WILL THATS WHY N LEAGUE ALSO DID not dare to form govt in kpk with jui f.though they could have of they wanted!its so so clear!
This pkpolitics site has been developed and maintained by Nawaz League's TUTTPOONCHIAYs…. Jitnee marzee Bakwaas ker loo our Noonion ke tarifain ker loo… Atlast it will be our loving country, Pakistan, which will suffer more due to these corrupt Sharifs…..
for all pml nooras,
I'm not really PTI member nor voter, but now i'm a supporter. You guys are just avoiding change. You finding no problem with thieves, because at least they have an experience of the theft. Those ideals who are committing themselves against the evil, you find them novices. Remember, you are doing what kufaar-e-makkah used to do with the holy prophet.
I know you can't understand, but please try – of course, miracles do happen!
what frigging change?pti also has low level knjers meerasis and looters and corrupt liberal fascists like other parties,which change pti morons are talking about?america gave you p[pl seats in kpk so you can change culture of kpk into meerasi cultre so jihad can end their,but you will fail and like anp Taliban will make you inshalah extinct
I agree with you.
so now youre down to comparing Holy Prophet PBUH with imran khan?
if you read your sentence again, youll find out what i mean to say.
also, youre saying that those who didnt vote or support imran khan are wrong because imran khan is committing himself against evil? isi liye wo LA court me pesh honay se chup gaya, isi liye he wrote in his book about him gambling, isi liye he calls others munafiq, isi liye he personally attack others? isi liye he has admitted of being a play boy? isi liye he has his kids being raised by a jew, becoming nn-muslims? itna he accha aur against all evils hai to pehle apne aap pe lage sub ganday aur ghatya ilzam ko prove wrong keray ya accept keray aur phir logon ko preach kernay niklay about change
for all pml nooras,
Regarding IK's preference on experienced people over the young, I think IK is just trying to learn from Nawaz Sharif. After all Nawaz Sharif has all the evils that he (and you stupids) criticize(s) IK against.
Don't preach what IK shoudl do, first and foremost try yourself to understand that what YOU must do. talk thereafter. Otherwise, dont' burry head in your own arse (or just do it ;)).
Salmn Mersai and PTI Inqelabiyo
Mian Muhammad Nawaz Sharif, next PM of Pakistan Inshallah, went to SKMH to meet IK. He said to IK, " Hun Araam ee?"
Your only treatment is that you dip yourself in BURNOL and may be the burning willl go.
Otherwise there is no treatment for you. Keep burning we welcome it.
And the language you use is typical of your leader Chanda Khan. So he drowned and so will you.
its true that only burgers mummy daddys and secular youth are with pti.thats why its a fact that in kpk the pti,s mandate is fake,they were given majority their by agencies so kpk people also become secular and stop doing jihad against America….this is a fact and writing is on the wall!do fair elections in kpk today and pti wont win more than 5 seats I assure u,IT WAS AGENCIES WILL THATS WHY N LEAGUE ALSO DID not dare to form govt in kpk with jui f.though they could have of they wanted!its so so clear!
Kanjar is always Kanjar
We always supported PMLN but this time we supported pti. It won election in oir village, i didn't notice any ridging in my town. Almost 90% in my area voted pti. I like their manifesto. They want to fix the system.
So now you must be eating Burgers.. per Hassan1995 and Deevav…:)
who supports PTI, please refere to the picture posted by BAWA on the same blog.
Atleat i do not belong to this group Alhamdulillah. However you may do as you wish.We are only watch this fan club silently but keenly.
@Deevav, I only said what Hassan1995 (Kid) and you guys are saying, that only Burgers voted and belong to this party.
This you woulld have to admit that PTI is followed by a special class. There are two types here. One in Karachi. Here people who were supporters of MQM and after failing to even acknowledge that they belonged to or supported MQM due to madness of Altaf, they switched the sides. They needed a break and they got it. In fact they did not want to support PMLN due to their hate for punjabl based party.
Others all over the country are those (majority) who have nothing to do with religion. They are of seculer school of thought. They follow west and want Pak like west. i mean in giving liberation to women like west has done. Else who can even think of sending their sisters or daughters to whole night long Dherna? These are special brand of people who do not care if these girls were actually facing threat in whole night dherna along with Lahori youth.
As for PTI and Karachi, I told you b4. It is not the Punjabi based party. NS was a coalition partner once with MQM. I attend that biggest combined opposition Jalsa in Katachi, where JI, NS, Altaf all were there. The Military action by NS cut off his roots from Karachi. You will disagree with reasons that is your choice.
PTI are new, people think this is an alternative given most young want to jump ship and join PTI. You cannot Stop.
As for Liberal, I am thinking why JI join PTI in Govt taking Education ministry.
As i know all PTI, NS, JI, JUI-F are pro Taliban , thats the common thread.
Think over , may be i am missing something about your analysis of Liberal, Girls etc…
There criterion of judging how Islamic a society is to count how many girls are wearing jeans and how many are in shalwar qameez.
Narrow-minded, fickle or just simply ignorant.
yar pakistan mein azzadi hai, sab faida uthatai hein, ager salman nai kuch kaha hai to kissi kon takleef kiyon.
Now that the dust has settled to some an extent. PTI leadership should seriously meditate over what went wrong. They lost steam in the final overs and and continuing to do so. They unrealistically reaised the hopes of their supports to skies, rather to the fartherst galaxy in the universe. They sincerely believed their immature leader that he would change Pakistan in one night.
Please wake up, this is a long struggle. If you do not realized your mistake, the party will vanish in the coming five years. IK said nations are not build by constructing roads but by education and now he has handed over the education ministry to already tested mullahs. The next blunder is handing over the most important finance ministry to the same mullah. It is yet to be seen what other ministries will go to other coalition groups. Allah knows what "hashar" they will make of KPK.
Now for God sake calm down and stop nonesense false propaganda. Enough of making people and yourselves fools.
This Salman Ahmed is a fool, he tried to make people fool and at the end made himself a fool. Our great leader his excellency Imran Khan Niazi, also did exactly the same.
This enthusiasm shows that people of Pakistan are fed up of the old corrupt politicians.
He was absolutely right in his arrogance..I would have been even worse if people start question my religion just in political combat!!
I wonder if Salman knows he actually sounds little a "stupid little brat"…this is why Nawaz won the elections and not Imran. Nawaz has civilized, smart, educated and a practical team with him, not some emotional misguided hollywood inspired fools. but then again, Salman was never our mentor or someone we ever looked up to. I guess he plays guitar and now he is learning to speak and socialize and very soon he will be forgotten!
Bey sura rock star…..Rock star my foot……Lanaat hai is per jis ko sur lee tameez nahi hai aur TV per bakwas kar ta phir ta hai…….He makes me laugh. What an idiot.,


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March 27, 2008
Getty Images
As if there were any questions before, it now seems almost certain that Kristian Huselius will not be back with the Calgary Flames next season. Two of the Flames' three core players, goaltender Miikka Kiprusoff and defenceman Dion Phaneuf, will be in the first year of new, expensive multi-season contracts that feature big raises over their current salaries. With both Huselius and centre Daymond Langkow eligible for unrestricted free agency, there is little question which of the two general manager Darryl Sutter will actively pursue.
That leaves the little matter of what can Huselius provide the Flames in the time he has left in the organization, after coach Mike Keenan benched him for Tuesday night's pivotal game against the Vancouver Canucks, won 3-2 by Calgary.
Keenan didn't want to discuss the reasons behind his decision, but it was apparent that he didn't think Huselius was going to make much of a contribution in a game that Keenan was determined to make physical. Keenan could come back with Huselius Saturday against the Edmonton Oilers, a team that isn't quite in Vancouver's league physically, and that would give both a chance to keep the working relationship cordial. Huselius needs a decent finish, if he wants to cash in on a statistically good year (65 points in 74 games).
Source: Globe and Mail
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Edited By Doug Farrar
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Edited By Jay Busbee
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Artist 2006 by FealasyWatch
©2006-2016 Fealasy
Yeeee.. That's us from generation 1980 allright.
*snoorrtt* (fullview or die trying)
*snoorrtt* (fullview or die trying)
your work is amazing, girl!
muy bello!!
reminds me so much things...
wow yor style drives me crazy!
I love the little creatures you draw.
man i wish my room was this cool!!
I want that mutant alligator.
the draw remaind me and my room...
hah.. looks like my room.. only... needs more laundry all over the place X3
It's awesome how you can make the most simple scenes really interesting.
So fun!
Awesome tha's all ^^
Awesome tha's all ^^
the checkered lizard thing looks cool to have :B
is fuckin' amazing!!!
those characters! oh my god superious - just what i wanted to see.
That's just awesome, as usual.
Awsome, as always.
cute little critters!! you're so creative
love the details. and the skull armsocks!
Pretty damn kool image love the whole image, your style is so kool damn it! *needs to think of better comments*
I just adore all the little creatures you create.
It's so COOOOOL!!!
i like the black cat ^^
OMG THERES AN EDD DOLL~ and a doll that looks like.. it .. like.. ate the neighbor kid before setting up for the photo shoot O.o;;
This is truely awsome
This is truely awsome
XD I wish my room was like that
Just like my room
I enjoy it
I enjoy it
I love the way you did the window. Excellent work
great work
looks like my room
very very cool.
These creatures are so cute! and the style is so awesome <3
this is totally like the inside of my head.........and room.
totally awosome
Im from 1989 and proud of it Oo
that is really good, this is so cute I love the stuffed alligator and how some of the stuffies goes out of the frame awesome job
yo it's on motherfucker with that
Ooooh! Cool!
This would make a fabulous poster I'd hand in my room. : D
that shirt.. those sleeves.. so fantastiskull.
oli likes how you can see dusty bugs in the light!
small detail but smooper cool!
i like to catch dusty bugs
and then to eat them
small detail but smooper cool!
i like to catch dusty bugs
and then to eat them
omg teh bloody dolly o__o
kk im 90 generation >_>;
kk im 90 generation >_>;
:applause:!!!! 1983inthehaaauuuuse (full of monsters) i love +watching you, it makes my days just a little brighter. ^_^
its so lovely and creppy all at the sametime
i'm not sure we all look like that but i definitely draw sitting like that and surrounded with tons and tons of junk!!
like always lovely work


text: Only stayed for one night - The hotel was very shabby but bedding was crisp and clean and bathroom was clean. Location of the hotel was not ideal due to passing traffic. Noisy music being played until quite late and noise during the night due to families leaving in taxis. Probably a stop-off point for the airport. Wrecked car in... More


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- Bachelor of Arts
- MA in Clinical Psychology
- Graduate Education
- Master of Business Administration
MA in Clinical Psychology Faculty
- Ron Alexander
- Deborah Allen
- Lisa Aronson
- Jo-Anne Blatter
- Margaret DuCharme
- Wendy Elliott
- Jonathan Eymann
- John Galaska – MACP
- Linda J. Holder
- Louise Jansen
- Klint Jaramillo
- Jeffrey Jarrett
- Barbara M. Katz
- Jacqueline Kurta
- Diana Lambeth
- Stuart S. Light – MACP
- Rudi Lion
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- Albert Munoz-Flores – MACP
- Suzanne E. Rapley
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Suzanne E. Rapley, PhD
Adjunct Faculty
Suzanne E. Rapley, PhD, has been adjunct faculty with Antioch University 1983. She is a Licensed Psychologist, Licensed Marriage and Family Therapist, ASSECT Certified Sex Therapist Diplomate, as well as an ASSECT Certified Supervisor. Dr. Rapley has been in private practice in Santa Barbara for over 25 years. Her areas of interest include relationships, intimacy, sexuality issues, sex therapy, gender identity, addictions including internet and cybersex issues; other areas of treatment include anxiety, depression. She sees individuals, couples, families and occasionally offers speciality, time-limited groups. Dr. Rapley has been a professor and trainer for over 30 years. She currently holds adjunct positions with Pacifica Graduate Institute, University of California, Santa Barbara and in the past was affiliated with University of La Verne.


text:
How to Make Your Voice Hoarse
Making your voice hoarse is a pretty easy thing to do. But there endless ways to do it. The easiest and quickest ways will be seen here.
1SCREAM! Go ahead and scream! Shout at people unnecessarily when replying or trying to catch their attention.
2Try to convince somebody to have a shouting match with you. Give it all you got.
3Whisper a lot. Whispering is bad for the voice; it puts strain on your throat and makes the airways close and tense up, making the whispering sound, but also
4Don't speak for a long time. When you DO speak after a long time, it will be hoarse because your voice will be like a toned muscle that hasn't been at the gym in a long time: un-flexed.
Questions and Answers
Give us 3 minutes of knowledge!
- Keep going and don't stop screaming or whispering or not saying anything unless it's an absolute emergency
- If you feel a burning or aching in your throat, that means it's working!
- This usually works well if you are trying to fake sick from school.
- Sing.
- Sing in a MUCH higher pitch than your comfortable with.
- If you continue to use your voice or scream or shout or whisper while your throat is burning, callouses and little lumps will form on your vocal chords and your voice will transform into something like a smoker.
Article Info
Categories: Boredom Busters
In other languages:
Español: hacer que tu voz se oiga ronca, Italiano: Rendere Rauca la Tua Voce, Русский: сделать свой голос хриплым, Português: Deixar a Voz Rouca
Thanks to all authors for creating a page that has been read 148,320 times.


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Yahwist and the Promise
As a literary artist the Yahwist1 has been compared to Homer and as a theologian to St. Paul. These accolades may be true but they may also prove a smoke screen for the beginning student of the Pentateuch. He wants to see the evidence for a Yahwist source beyond the texts of Genesis 2–9. We could, of course, follow the lead of most introductions to the source hypothesis of the Pentateuch and list all proposed Yahwist style and theology. Such a method is comfortable. It adopts the findings of some great scholar and assumes that the evidence for identifying the Yahwist writer throughout, the Pentateuch is the same or similar to that provided in the preceding analysis of Genesis 1–9. The critical reader, however, will want to test these assumptions. He will want to know what kind of data, criteria, or evidence play a role in the identification and understanding of the Yahwist in the patriarchal, exodus, and wilderness traditions.
It is obvious that we will not have opportunity here to discuss each proposed Yahwist passage. Nor will we have a chance to treat many passages where the separation of Yahwist and Elohist sources is problematical. We shall therefore choose typical and normative materials to illustrate the literary character of the Yahwist subsequent to Genesis 2–9. In so doing we hope that convincing connections with the Yahwist texts of Genesis 2–9 will become apparent and the governing characteristics of the Yahwist's total literary work will be revealed. At the same time we do not want the bold outline of the Yahwist which follows to obscure the fact that many contours of the Yahwist literary source have become blurred in the course of textual redaction and transmission.
Genesis 18 provides a profitable example of Yahwist literary formulation. In this chapter we can discern many of
1. The writer of the Yahwist literary source is usually designated the
Yahwist as though he were a clearly identifiable individual. For the sake of
convenience we have preserved this traditional designation. See P. Ellis,
The Yahwist (Notre Dame, Ind.: Fides Publishers, 1968) for a recent anal-
ysis of Yahwist style and theology. This book provides a complete text of
the Yahwist.