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Activity from in the news
Swagbucks Is On The News Again!
Posted by Team Swagbucks on Wed, March 3rd, 2010 at 8:00 am
We've had a lot of excitement around here as of late and this blog will just feature more of the same. Every now and again, Swagbucks will end up on the news. With the launch of Swagbucks 3.0, me and the rest of the team at Swag Central were wondering how long it'd take for us to be on the news again with the new design and everything we had introduced.
Turns out, not long at all! ABC affiliate, KMBC 9 in Kansas City ran a piece on Swagbucks as part of reporter and anchor, Dion Lim's 'Dion's Daily Deal' segment. A big thanks to Dion Lim as well as Swagbucks member and CouponMom, Chrissy Pate!
See the video of the segment here!
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The Official Report is the full and authoritative written report of proceedings in the Scottish Parliament and its committees.
This includes:
The Official Report is substantially verbatim. Shortly after the Parliament's first meeting in 1999, the Presiding Officer explained that 'substantially verbatim' means that repetitions and redundancies should be omitted and obvious mistakes should be corrected while maintaining the flavour of the speech. Inevitably, some editorial judgment has to be exercised; for example, the speaker's tone of voice or use of physical gesture may add to or even contradict what he or she is saying, and this must somehow be communicated in the written text.
Members may read the transcript of speeches
that they have made in the chamber or in a committee prior to publication. Speeches
made in the chamber are generally ready an hour and a half after they were made.
To inquire about speeches made in committee, members should contact the
Official Report office on x85447.
Speeches may be read in person, by visiting us
in room T2.20, or we are happy to email them to you. Please email email@example.com or call us on 85447 to request this.
We are happy to provide draft speeches in larger font sizes and to make appropriate arrangements with members or their staff who have specific access requirements.
If a member or a witness is concerned that what has been reported is not an accurate reflection of what they said, they may contact us to ask us to review the report of that meeting. They may suggest corrections in person, by email, or by sending us a marked-up printout. Please do not send a track-changes file.
The editor will consider suggested corrections in the light of our terms of reference and house style. Speakers may not change the sense of what they said or make substantial amendments if what has been reported is an accurate reflection of what was said.
If approved, suggested corrections will be incorporated in the final archive edition, which is published in due course on the Parliament's website.
If a member supplies incorrect information that the Official Report is unable to correct under its terms of reference, they may take advantage of the members' corrections mechanism recommended by the Standards, Procedures and Public Appointments Committee's 7th report (session 3).
The members corrections guidance gives more detail about the mechanism and how to request a correction.
View members' corrections to the Official Report

subset: commoncrawl


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I hope you all had a shitty Valentine's Day. I mean that, in the nicest way possible.
I hope it was depressing and filled with regret. It would tickle me the appropriate pink if the rose you bought wasn't red enough, the words in the sappy card rang false and that the chocolates you ate gave you both diarrhea. Maybe if you spent your Valentine's Day bitter and pissing out of your rear end you'll rethink going along with the charade next year. Maybe you'll remember to treat those you love with the kind of adoration the rest of the year expected of you on a day Rite Aid's around the world decided they needed a boost in sales.
If its worth the effort when you're told it matters most, its worth the effort the rest of the fucking time.
A lesson the Kings could learn. Monday against the St. Louis Blues the Kings played like they did in the playoffs. Perhaps staring down Ken Hitchcock's chin waddle swathing over the opposing bench fooled the team into thinking it was the playoffs again, back when every game was really damn important. But even on the days that the National sponsors aren't singing your praises, the same tenacity and dedication to every moment is required. Even against boring, useless franchises like the Columbus Blue Jackets the game must be played like these guys are what stands between us and the Stanley Cup.
I'm excited the Kings are home again. It's been too long. Three day interims between games at this point is longer than a half season lockout is after a summer. As I expect the Kings to play tomorrow, so shall I be in the stands. I'm ready for this crappy start to the season to succinctly be snuffed out like so many kittens beneath an drum of oil tumbling down a hill. Loud, rowdy and raucous. Let team and fan be a reciprocal echo.
Friday night games are rare. You ain't working Saturday. You don't have to pretend you care about Sunday church in the morning. Get there early. Stay late. Burn it all to the ground.
I'm feeling it. The engine is revving and the team I saw on Monday is waiting to be unleashed again. Cascading waves of aggression towards the net. Stubborn sticks in narrow lanes. Attack, defend, recovery, no recoil. Swift and decisive plays.
I've got nothing coherent to say. I'm a snarling fiend.
Tonight the leash comes off.
Categories: L.A. Kings News


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Painting over mould and mildew
Most homeowners know what bathroom mildew looks like. It's that dark and mouldy looking stuff that appears around shower curtains, tiles or above a shower wall. This is not only something that is an eyesore, but is also unhealthy. Not many people have an idea on how to paint over mildew when redecorating their bathroom. It is actually possible with the use of zinsser paint.
Mildew is a fungus that continues to grow on moist surfaces unless it is removed. It also causes peeling paint. It this is what's happening to the bathroom, then the owner must remove the mildew and peeling paint prior to repainting. The mildew is removed by mixing a solution of water and bleach, and then spraying on the affected areas. The spray should be applied in every place that has black mildew marks. This ensures the mildew is dead before the bathroom walls are painted. The affected area has to be allowed to dry before proceeding to the next step.
The next step involves scraping all the peeling and loose paint and filling any dents or cracks with a spackling compound. As soon as the compound has dried, it should be sandpapered until smooth. If necessary, it can be re-coated with additional compound as it often shrinks after drying. Any spackle dust is removed using a damp sponge. The walls are then primed using a high-quality primer that should be allowed to dry. They are then painted with an eggshell finish latex paint.
Some homeowners wonder why a flat paint cannot be used. This is because the walls will get exposed to water, facilitating the growth of more mildew in the future. Most probably the mildew will return as it thrives in dark and damp environments. To help stop the mildew returning buying new, brighter bathroom chandeliers or a high watt bulbs willl light up the area. As mildew does not enjoy being exposed to bright lights, having them will discourage the growth of them. If the homeowner observes the mildew returning, he or she should wipe the walls using waterbleach solutions and a damp sponge. This prevents it from doing a lot of damage to the walls. A high-quality paint that is mould-resistant not only retards growth of mildew, but also holds up to repeated washing extremely well. Such paint is also low odour and is available in a wide range of colours.
More information on bathroom chandeliers
The problem may be severe and persistent. If so, the bathroom owner should ask paint store to add a packet of mildewcide to the paint before shaking the can. This way, the paint and the mildewcide will be evenly mixed. For those using at true kitchen or bathroom paint, they contain mildewcide in them already, but more cannot hurt.
When treating bathroom mould, it is vital to ensure that tiles and grout are clear of it. In some cases, the homeowner may have to replace grout or tiles. These are the cases when a beach treatment is used, and one finds that the mould does not seem to respond to the bleach. A probably reason for the lack of response is that the bathroom mould has grown under the tiles, meaning the homeowner will have to dig it out and treat it in order to eliminate it.
Find our more about how paint such as anti graffiti coating can be used to keep your property looking clean.


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Slovenia's Economy Continued to Shrink in First Quarter
Slovenia's economy continued to shrink this year as austerity measures in Europe cut demand for its exports, the central bank said.
Monthly indicators suggest that Slovenia's gross domestic product was lower in the first quarter, Banka Slovenije in the capital Ljubljana said today in its monthly report. The euro-region nation's economy expanded 2.1 percent in the first quarter a year ago, before entering its second recession in three years in the last quarter of 2011, when GDP contracted 0.7 percent from the previous three months.
"Weak activity in the euro region is affecting export demand, as well as manufacturing and we are seeing lower revenue growth in services, such as transport and storage," the central bank said.
GDP is forecast to drop an annual 1.2 percent this year on weak demand in Europe and in its home market, according to the central bank.
The shrinking economy may complicate efforts by the government of Prime Minister Janez Jansa to cut public spending and follow the German-inspired austerity program in Europe that is meant to allay investors' concern over the euro-area's debt load. The government pledged to reduce the spending by about 800 million euros ($1.04 billion) to below 3 percent of GDP by year's end.
The government needs to avoid "any extraordinary expenditures, which last year contributed 1.3 percent of GDP to the shortfall," in order to meet this year's task of narrowing the gap, the central bank said.
Last year, the government deficit surged to 6.4 percent of total output from 6 percent a year earlier as the previous administration of premier Borut Pahor boosted the capital of Nova Ljubljanska Banka d.d. and helped other state-owned companies.


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Hybrid Theatres
It feels great to be back on board www.HospitalDesignTips.com after a while as we have been extremely busy with our assignments on greenfield healthcare projects and despite our best endeavours we had to keep all our energies focussed on our clients and best protecting their interests.
A big Welcome back to all our regular visitors and readers of www.HospitalDesignTips.com, we hope to keep you abreast of the latest development in the healthcare industry through informative new articles regularly.
I have been meaning to expand my article on Operating Theatres to include Hybrid Theatres and this article will focus on that subject.


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Reviews - The Pendle Inn4
Barley Village, ,, Burnley, BB12 9JX
Price From 133.13 AUDView Rooms
Customer Reviews
Our reviews are from genuine LateRooms.com customers. We verify that the customer has stayed before leaving a review.
Very Good
89% would recommend it to a friend
Customer service
Food quality
Room quality
Very friendly staff, well-appointed rooms, comfy bed - just a shame there was no breakfast on the premises.
And that we weren't staying longer.
Read More Less
great room and facilities
really impressed with the rooms - much more than a hotel room.
a great little suite with kitchenette and lounge area. Very friendly staff and fab food at the inn. would definitely come again and would recommend !
Read More Less
Excellent place to stay
We really enjoyed the Pendle Inn.
Lovely and cosy, plenty of room and very clean. Friendly staff at the adjoining pub. Perfect if you are a walker but just as good for us going to a family get together nearby. We would definitely book again. Thank you.
Read More Less
Necessary journey for funeral, inn recommended by friend
A lovely Inn in a beautiful area with 6 terraced cottages in yard.
Very clean and comfy and warm, with some nice touches. We didn't eat there but had a drink in the bar, which was very pleasant. No breakfast provided but there was a tiny kitchen in the room for snacks. Main fault was the thinness of the walls- only there for one night but we could hear TV very clearly in the adjoining cottage, and when I was kneeling next to our TV, working out how to switch it on, I realised that the connecting door behind TV had a big keyhole where you could clearly see next door's room and TV. Had someone walked past, you would have seen them too. There was also a connecting door on the opposite wall with a similar big keyhole, but this cottage wasn't occupied.
Similarly I noticed when I was in the bathroom - opposite front door - there was a big key hole there too, and looking through it from outside, you could see straight into the bathroom and toilet. Very embarrassing if you had door ajar. The downstairs bathroom was inconvenient too, as my husband's illness necessitates him getting up several times a night, so he had an uncomfortable night on the settee, while I had a lovely bed to myself upstairs. But the couple who run it were very nice and helpful, allowing us access earlier than the stated 2pm, to enable us to change and get to funeral in Burnley. The staff also assisted us with help to negotiate the TV, coming across in the pouring rain. Sadly we would probably not come here again because of the above reasons. We accept nothing can be done about the bathroom, but the sound carrying and 'spy-holes' need to be addressed, as I have noted other comments on your web site about the sound through the walls.
Read More Less
Amazing little place
Lovely little place, very clean and very friendly
Self catering rooms - low in kitchen utensils
no breakfast in charge
The Pendle Inn
It were very nice and not haunted at all
Pendle Inn, Barley - Customer Comments
The accommodation in the chalet was acceptable but a good night's sleep was not really an option as there are skylights with only flimsy blinds fitted which meant that the room was flooded with daylight from about 05:00 hrs.
Perhaps the proprietors would consider fitting blinds made from more substantial fibre such as 'black out' material. The food in the restaurant was good as was the service there. Re the so called friendly pub itself, namely 'The Pendle Inn' which my companion and I only visited on the first night of our stay...........this was, I am very sorry to say, not a very welcoming establishment and any friendliness and warmth on the part of the proprietors and bar staff was very pointedly reserved solely for the locals of the area and my overall impression was that it is cliquish and to be avoided by visitors who are made to feel like interlopers.
Read More Less
Good accommodation for walkers and touring would use again
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The CMMS product line is built upon standard off-the-shelf commercial hardware and core software infrastructure that decreases the time required to develop new unmanned control systems and enhances future technical upgrades because the system architecture is based upon well defined industry standards. Additionally, with the CMMS product line, pilots will be able to operate a variety of dissimilar unmanned platforms using the same informational displays and control features, thereby improving mission effectiveness while reducing training requirements."This demonstration validates our approach to common, modular, multiplatform mission control systems," said Doug Valenzuela, Northrop Grumman's program manager for the Ground Station Technical Refresh program. "We were able to reuse components from proven programs and integrate them into a common standards-based infrastructure to establish a baseline that will meet the requirements of multiple programs. This is truly a huge step toward meeting the objective of a common UAS mission control solution."
CONTACT: Carissa Kwan 310-813-3811 email@example.com


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Oxbow Sunrise by StevenDavisPhotoWatch
©2012-2016 StevenDavisPhoto
Sunrise at Oxbow Bend in Grand Teton National Park
Another beautiful scene with fantastic light/colours Nice work
Woooaaahhhh this is so pretty! I love the way you took the photo, everything looks so sharp and clean. And great composition!
Beautiful light. I have downloaded it for a desk top too. Hope that's ok with you.
Beautiful shot!
Amazing nature capture! The warm color tones are great. The mist gave it a fantastic mood.
I do believe this is THE most stunning autumn photo I have ever seen! Thank you for allowing downloads - it is my new wallpaper for the season
Amazing scape!
Incredibly beautiful. Thank you for making this downloadable, so I can view it later!
Perfect, nice pic..
Wow, that is something special.Superb work!
Brilliant color my eyes are burning!
Awesome capture!!! We were there last year at this time... what a great photographic location.
What a great shot! And Location...well done
Fantastic light!
How brilliant is this????????????
Simply amazing. The detail is outstanding and the colors bring serenity to mind. Love this!
Stunning shot!
Stunning work
Nice Steve! Never seen a wide shot of Oxbow, very cool!
The colors are so gorgeous, bright and warm! What a great capture. That's why I like autumn so much
Fabulously beautiful colors and light!
Sunrise serenity ~ a lovely atmosphere in this gorgeous capture! Coco
beautiful colors....
A wonderful golden and beautiful autumn picture
it loooksss awsommmm
That was worth getting up for. Great shot! Fav.
wow. what a view!
very nice - good to see a different perspective on this
You are the luckiest man to see such beautiful things : )
Superbe photo ! les couleurs sont extraordinaires.
This is just fantastic!
Wow! The color and the low lying clouds are amazing.


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AnalAcrobats Chastity Lynn Gets Ass Fucked by BBC
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that is a very large piece of meat. A+ for the effort.
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Chastity Lynn any Lex Steele two of the best. She did a great job trying
to take that all of monster in her ass.
to take that all of monster in her ass.
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Recipe by: user0220
Original recipe yields 16 servings
Based on a 2,000 calorie diet
Sort stores by
Ready In
This simple, creamy appetizer takes its cue from English tea sandwiches.
Sugar, cinnamon, and chili powder give roasted almonds an addictive kick.
A zesty marinade takes store-bought pretzels to the next level of snack heaven.
Have made this a couple of times and the hubby and I love it :)
I made it exactly how the recipe was written. Most who tried it said it was the best they've ever had! Nice job to the original poster!!!
I added an extra avocado to cut down on the spiciness. My family loved it!
Best guacamole I've had yet!


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If you have a website of your own for either business purposes or just for fun and information, you still have the main goal of trying to draw as many people as you can to your site to see what you are doing or what you have to offer. Over the years, the technology involved in marketing on the Internet… read more →
Twitter is a very large social network which is being used by over 130 million people. This is a wonder why it is so difficult to ignore the site. I have being questioned by so many people as to why there is no main strategy for tweeting effectively. But, to prove them wrong, I have decided to write this article… read more →
Pretty much a big question for all the internet marketers these days, is not it? Small micro niche sites focused on a single long tail keyword (With moderate search volume and little content) used to work pretty good earlier. These are never high earners, but it's pretty easy to make one and hence, you can create many such sites and earn quite a residual income on absolute passive mode.
Now, the last line may be tempting and is probably the most used one in sales copies of micro niche site sellers. Things like "micro niche" sites may have worked earlier, but the big G is even more vigilant these days. Google hates thin content sites and micro niche sites are anything but content rich. Just a few articles are thrown all over the site and a micro niche site is made for you. Google does not like those anymore, mate!
Look at your blog as a seed and then a plant. It needs to be planted in good soil (profitable niche) and near a good stream of water (traffic sources).
Please use your imagination with me for a bit.
Good soil – Profitable niches
Lets talk about the soil. I'm referring to the soil as being the profitable niche you may be interested in
Introduction Blogging can be a great way to make money in very little time, simultaneously achieving a stable and secured career. This may sound fantastic however in reality blogging requires a lot of effort and patience. When you are new and have just started your career as a blogger, you will face great challenge for coming up with new ideas… read more →
The beauty about affiliate marketing is that you don't need your own products to sell to be successful, and when you do start making money online you will realise that online marketing is not a scam despite many 'get rich quick' schemes making it appear so. It is a fact that money can be made online as long as you… read more →
In this article we will discuss the basics of writing quality content. Nowadays, the internet is flooded with blogs that are to a large degree pointless and do not add any value to the table (let's not take into account those blogs that are being used as diaries), and it has become hard for people to get the information they… read more →
Everyday new blogs are created under different niches to provide information about products and product reviews, online services, marketing and so on, to their valuable readers. However, no matter how good your blog content is, no one will find you if you don't send tons of people to your site. In this article, I will show you 4 ways to… read more →
Making money from your site, for some may sound like a dirty business, for the Mommy bloggers out there who just want to share their thoughts, opinions and ideals with the world making money from their site may be inconceivable, but the fact of the matter is that even though they are blogging for the love of it, their hosting… read more →
Ok, you have launched your Blog, you have made it content rich writing useful information and guesting high quality posts, you have done great SEO work and now you have a good amount of traffic from the SERPs. Now it's time to monetize your Blog! You may ask: How much traffic should I have to get started monetizing my blog?… read more →


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Pistol Storage
View Single Post
November 13, 2012, 08:47 AM
Senior Member
Join Date: January 22, 2005
Location: Kentucky
Posts: 1,733
Yep...I do that with mine...a good wipe down with a heavier oil...30 wt car oil works well, then into the safe. No fingerprints to start the rusting process after the wipe-down.
Be careful what you lay your guns down on...some fabrics can and do hold moisture...leather has acids in it due to the tanning process...sheepskin "gun sleeves" hold moisture...
I use a plane wood rack, finished in two coats of poly....and I make sure that the gun room does suffer from extreme temperature variations to avoid condensation issues. In that regard, a garage is not a good place for storage., but your closet might be good, no matter what your wife says! Rod
Our Flag is unfurled by the dying breath of our patriots in uniform. Cherish and honor it, defend what it stands for or get the hell out. Our Freedoms are not free, they've been paid for many times over.
USAF Forward Air Controller, 5th Spl Forces,
An Loc, lll Corps, RVN, 69-70, Vietnam Vet '69-'73
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I struggled for years as a child (in school and at home) and as a adult (in college and graduate school, at work and at home) with ADHD. Many people thought I was messy, lazy and unfocused. I kept missing deadlines. I was properly diagnosed this past January and, with medication, counseling and skills coaching, I finally feel that I'm performing up to my abilities. It's still a battle everyday, but I know now what I'm up against and how to deal with it.
Does anyone know if diet and exercise can be as effective as drugs in the control of ADHD? Do you always have to have drugs involved to control? thanks
My 32 year old son took Ritalin and excelled in school, even after he quit taking it at puberty (which is the usual procedure). In college and later he has continued to have attention problems which frustrate him. He did graduate Phi Beta Kappa - but forgot to meet friends at the proper time when he was studying in Europe. ADD is very hard on relationships.
On the food (sugar & dyes, etc.) - we tried him on that special diet for a month when he was 6, but it made no difference. Caffeine and sugar still make him sleepy, not hyper.
There is no substitute for a well qualified doctor - they do not prescribe indiscriminately.
What is missing from the clinical evidence for treating ADHS with drugs (or anything else, for that matter) is meaningful, long-term evidence that treatment actually improves "life" outcomes. Sure, the kids sit down in school, and the parents have a more harmonious household, but do children treated for ADHD turn out to be more successful as adults? That is the question that remains to be answered. Anecdotes, however tear-jerking, are not evidence.
My son, 7 years old, has been diagnosed with ADHD over a year ago.
We stabilized the home life, got him the right meds (Strattera), he received support from the school and is attending family therapy. In my opinion, the meds and a stable home life go hand and hand. It is tough to make progress if there are significant stresses at home. This goes for all kids but especially mine who is ADHD. We have seen great progress over the last five months.
Regarding Strattera, it is a non-stimulant unlike Adderall XR. Adderall was a bad fit for him and I had concerns over the stimulant properties. Strattera takes 3-6 weeks to get into the system fully but we have found it to be very good for us. Just on Dad's take.
Wake up people, ADHD is real. My son is a gifted, 6 yr old with ADHD and the medicine (Concerta) improves his life by 100%.
The "special diet" or "counseling", etc.. Will NOT help if your child really has ADHD then meds are required. Would you keep medicine from your child if he had cancer?
Many parents hate giving their kids drugs, and I can understand that, but if your doctor recommends it, DO IT. If your too stubborn to get your son/daughter tested, then you've got a problem.
The WSJ Health Blog offers news and analysis on personal health, new research and health news.
It includes contributions from staffers at The Wall Street Journal, WSJ.com and Dow Jones Newswires. Write to us at email@example.com.


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Chicken Salad Bake Recipe
4 5
This amazing twist on the classic chicken salad is sure to impress guests. And at around 300 calories per serving, this main dish is one you can have seconds of without feeling one bit of guilt. Darla Germaux - Saxton, Pennsylvania
- 4 cups cubed cooked chicken breast
- 2 celery ribs, thinly sliced
- 1 small green or sweet red pepper, chopped
- 1/2 cup sliced water chestnuts, halved
- 1/2 cup sliced fresh mushrooms
- 1/4 cup finely chopped onion
- 1 cup Miracle Whip Light
- 1/2 teaspoon garlic powder
- 1/2 teaspoon pepper
- 1 cup soft bread crumbs
- 1 tablespoon butter, melted
- 1/4 cup shredded cheddar cheese
- In a large bowl, combine the first six ingredients. Combine Miracle Whip Light, garlic powder and pepper; stir into chicken mixture.
- Transfer to a 2-qt. baking dish coated with cooking spray. Bake, uncovered, at 350° for 20 minutes.
- Toss bread crumbs with butter; sprinkle over chicken mixture. Top with cheese. Bake 7-10 minutes longer or until heated through and top is lightly browned. Yield: 6 servings.
Originally published as Chicken Salad Bake in Healthy Cooking October/November 2008, p47
Reviews for Chicken Salad Bake
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Respond to Review of Estragon and Rosmarin
by wildfang
Review: We went to the Estragon und Rosmarin in Weimar on our tour through central Germany and really enjoyed it. The staff was very friendly and helpful about vegan options which weren't marked on that day. We had a very tasty corn tomato soup and salad as well as organic juice and hot chocolate and even got a little discount. There is an organic shop attached to the cafe aswell but since it was Sunday, it was closed unfortunately. The cafe is also conveniently located right on Herderplatz so you can marvel at the church and watch people walking by.
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RealNetworks, Inc. Releases Update to Address Security Vulnerabilities
Updated October 25, 2007
RealNetworks has issued a fix for a vulnerability identified as a malicious web page which affects the import method of an Active X control to cause a stack overflow in the Realplayer. CVE-2007-5601. This posting is applicable to versions of the product downloaded before October 25th, 2007.
RealPlayer 10.5 and RealPlayer 11 beta users should install the patch per the instructions below to address this security vulnerability that aims to cause buffer overflow that could provide the potential for an attacker to run arbitrary or malicious code on a users PC.
RealOne Player, RealOne Player v2 and RealPlayer 10 users should upgrade immediately to RealPlayer 10.5 or RealPlayer 11 beta following the instructions below.
For Windows XP, Windows 2000, Windows 98, Windows ME:
Please click here to install the patch for RealPlayer 10.5 and RealPlayer 11 beta.
Please click here to upgrade your player for RealOne Player and RealPlayer 10.
For Windows Vista:
Please click here to go to download a new player from the web.
Macintosh and Linux versions of RealPlayer are not at risk for this vulnerability. In addition, RealPlayer 8 and earlier versions of RealNetworks software for Windows are not affected.
We are committed to providing our customers with timely and comprehensive information about our software. As such, we encourage users to check this site periodically for the latest updates.
Windows Players:
If you are on Windows Vista, please click here to go to download a new player from the web.
RealPlayer 10.5 and RealPlayer 11 beta customers can get a patch to correct this issue. Please click here or to update your Player.
RealOne Player (English only), RealOne Player V2 and RealPlayer 10 customers require a full download to correct this issue. Please click here or use the following steps to upgrade your Player:
Details for Potential Vulnerabilities:
The identified vulnerability is a malicious web page which affects the import method of an Active X control to cause a stack overflow in the Realplayer. CVE-2007-5601
RealNetworks would like to acknowledge cert.org as well as those who subsequently worked with RealNetworks to correct the vulnerabilities.
RealNetworks Inc. endeavors to provide you with the highest quality products and services, but cannot guarantee, and does not warrant, that the operation of any RealNetworks product will be error-free, uninterrupted or secure. Please see your original license agreement for details of our limited warranty or warranty disclaimer.


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This is a great place to stay if you only need a studio. Some views are ski slope. The best part is it is considered ski-in, ski-out because it is so close to the slopes. I have stayed here for years and always slope-side for the view. Remember it is a studio but all usually sleep four. For the price,... More
Top-rated Attractions Nearby
Top-rated Restaurants Nearby


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Chris Colfer watercolor by mssirpercyWatch
©2012-2016 mssirpercy
my interpretation of the most exquisite face I've ever seen
color pencil
color pencil
I saw your lectures a lot before, but until today I know you like him too. What an amazing art!
I love your technique with the watercolor pencils! I am new to using the water colored pencils so how did you get the splatter effect while using the pencils?
love it!
did you use white colored pencils to draw the eyelashes before you painted so there would be white area left? It's beautiful by the way.
i believe i just made sure not to paint in those areas and then reinforced it with white paint. the shiny parts are done with gel pen. thanks for the comment!
" most exquisite face I've ever seen" no truer words have ever been spoken.
This is stunning, well done!
have you considered posting this on tumblr? people with FLIP over there
This is stunning, well done!
have you considered posting this on tumblr? people with FLIP over there
oh my, thank you!!! ahhh perhaps i will i just really don't have much of a footing on tumblr...it's just 99.9% reblogging other people's stuff with me :/
This is really gorgeous work. Good job
oh mah glob this is just a freakin mind blow!! 0o0
WOW! This is beautiful! Excellent work!
this is the best thing ever omg
i love this so so much
I just...this is one of the most beautiful things I´ve seen
that is incredible...
So. Effing. True.
He has the most beautiful place on the planet. It's seriously insane.
And also, GORGEOUS drawing!
He has the most beautiful place on the planet. It's seriously insane.
And also, GORGEOUS drawing!
yay thank uuu
This is absolutely gorgeous and I want it on a canvas and on my wall and in my house forever and ever. I was hoping I could get it printed on a bigger sized canvas than 12 x 18 in, though. Hmmf.
thank you! that means a lot ahh i wish i could upload a bigger image size, but I'm 400 miles away from home and all my original artwork hope you'll still consider!
This looks a lot like the style of Agnes Cecile's pieces!
Wonderful job, this is absolutely stunning
Wonderful job, this is absolutely stunning
just stunning O.o
This is amazing...
i love this one


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text: British ColumbiaThere are so many adventures to be had in British Columbia; from stunning mountain landscape to rivers chock-full of salmon and trout for world class fishing, crystal clear lakes to dense lush forests and abundant parks perfect for hiking and biking. Part of Canada's incredible experience is the wildlife. Plenty of opportunity for whale watching and bear watching, in particular during the Autumn when grizzly bears gather to fish for salmon, an awesome experience to behold. The province generally enjoys a temperate climate but where there are mountains, there is snow making for the amazing skiing. BC offers not just outdoor adventure but a cosmopolitan culture, world class exhibitions, shopping and fabulous dining making it the perfect diverse destination.


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Start a video visit, and find the birth control that's right for you.
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The two-year / graduate school scholarship is available for those who have two academic years of college remaining. If you are a Senior looking for a way to attend graduate school, Seattle University Army ROTC can provide you with an opportunity to reach your academic goals, and become an Army Officer in the process.
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According to local newspaper the Daily Mississippian, an estimated 20 Ole Miss football players were in attendance for the Tuesday evening performance of the theatre department's first show of the 2013-14 season — The Laramie Project, which continues through Oct. 6 at the Meek Auditorium. Several used derogatory slurs such as "fag" and heckled cast members and the characters they portrayed for their body types and sexual orientations.
The theatre department's performance report stated that house director Lyda Phillips, a theatre major and athletics ambassador, became aware of the football players' reactions and called a coach, who then contacted the Department of Athletics associate director of academic support, Drew Clinton, to report to the auditorium.
The football players, who were required to attend the show for a freshman-level theatre course, were asked to apologize to the cast following the performance. One undisclosed football player apologized on behalf of the group, causing two cast members to cry.
Cast member Garrison Gibbons — the only openly gay student in the production — shared his initial recounting of the incident with Playbill.com, which reads, "The audience was mocking the portions of the play that revolve around hatred and serious subject matters. Then, people laughed and mocked us as actors. I felt, every time I entered, I was being laughed at for being gay. In one of my monologues my character says, 'I'm 52 years old and I am gay,' and there is a pause. In that pause, I felt so much judgment and then came the laughter. Towards the end of my monologue, I realized people in the audience were using their iPhones to photograph me on stage with flash. Throughout the performance it got worse with comments overheard about the actors, and flat out laughing at the words 'fag' and 'queer.' Also laughing at the comments that Aaron McKinney, the murderer, said about the gay person he killed. It would be easy to generalize all the blame of this on the 20-30 football team members that were there, but they weren't the only ones. Many other people responded this way, too."
"As Chancellor Dan Jones has said, we have our good days and days that aren't as good. Tuesday night in Meek Auditorium was an example of the not as good. However negative these events have been, the Department of Theatre Arts hopes that the individuals involved, and all members of the campus community, can learn from this experience. Whenever hate speech is used on campus, we must all take time for reflection and endeavor to ensure that those who would use this type of language are educated in how hurtful and dangerous it can be. As The Laramie Project suggests, every time one uses hate speech, whether it be based on gender, race, sexuality or any other discriminatory measure, you are planting the seed of violence. This University must remain vigilant in its efforts to rid our campus of this bigotry and hatred.
"The Department of Theatre Arts has been in contact with the Chairs of the Bias Incident Response Team, members of the office of the Vice-Chancellor for Student Affairs and members of the Athletics Department. All units have been very supportive of our students and are treating the matter with the utmost seriousness."
"We certainly do not condone any actions that offend or hurt people in any way," tweeted Hugh Freeze, the head coach of the Ole Miss Rebels, Oct. 3. "We are working with all departments involved to find the facts."
The Ole Miss athletics department did not issue a comment, but Rory Ledbetter, the play's director and theatre faculty member, told the Daily Mississippian that he received an emailed apology from a member of the athletics department. Dean of students Sparky Reardon said, "I am extremely sorry to hear that this happened. We are still looking into the specifics to determine what happened."
In his recount of the evening, student actor Gibbons also wrote, "Ole Miss as a student body needs to have a better understanding of equality and a better understanding of the LGBTQ history since this is the LGBTQ history month. I don't want to walk around this campus in fear. I want to be able to be myself, be open, and not fear judgment. I hope one day that dream can become reality."
"For me, it was shocking," Gibbons told Playbill.com by phone Oct. 3. "I've never felt something like this on this campus."
Although Gibbons said that he was disappointed by the negative response from the athletics department and many others in the audience on Oct. 1, he told Playbill.com that he has experienced nothing but support since the news broke.
"I've gotten over 50 messages of support on Facebook," he said. "I've only received support. Last night, when we did the show, and we walked away, [audiences responded that they] 'loved it'… We [now] understand the hope and the pain that these [characters in The Laramie Project] were feeling."


text:
Link In Action by R-LegendWatch
©2012-2016 R-Legend
I thought he was wite
You make a badass Link!
Nice pose!
What an awesome photo! you look great!
this is awesome!
haha Oh wow nice!
You're expression is perfect!
is that a metal master sword?
It's made of wood.
huh not as cool but still cool...if only it was metal like mine
great cosplay and pose XD
Looks great!


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text:
Upgrade SCCM 2007 SP1 to SP2
Step1: Uninstall the Windows Automated Installation Kit (AIK) version 1.1 from the SMS Provider computer and then restart the computer before beginning the upgrade process.
Step2: Insert SP2 Media on SCCM 2007 Server. And run Setup.exe
Figure 1
Step 3: Select Upgrade option
Figure 2
Step 4: Select the Patch where you want to download the Updates and click on next
Step 5: Click Next
Step 6: On Successful download of Updates Click on Next
Step 7: Click on Begin Install
Step 8: On Successful Installation click on Next
Step 9: Click on Finish to Complete SCCM 2007 SP2 Upgrade
On Successful upgrade to SCCM 2007 SP2 you can run R2 to get some additional functionality
The following features are new to Configuration Manager 2007 R2:
For more information, see About Unknown Computer Support for Operating System Deployment.
For more information, see About Multicast for Operating System Deployment.
Operating System Deployment Using SCCM 2007
Step 1: Install WDS on SCCM 2007 Server or on Different Server. On Successful Installation Configure WDS Server
Step 2:
Once WDS is Configured Click on the
Configure DHCP Server and If DHCP and WDS are in Same box then click on Check box marked in red. If DHCP and WDS are in different box uncheck both the box below and configure DHCP Option 66 and 67.
If WDS is installed on Different Server in SCCM 2007 Server Console Add the Server and Assign PXE Service Point.
If SCCM 2007 and WDS is running on the Same Server then Assign PXE Service point role on the SCCM 2007 Server.
To Confirm Successful Installation of PXE Service Point please confirm on PXE Setup.log c:\Program Files\Microsoft System Center Configuration Manager \logs
Enable Unknown Computer Support on PXE Service Point. Right Click on ConfigMgr PXE Service Point and click on properties
Injecting Drivers
Download Drivers and install on a folder. From SCCM 2007->Operating System Deployment->Click on Drivers ->Import
Click on Next
Select the Drivers if you want to inject in Boot Images.
Once completed you will be able to see drivers in Drivers Tab
To Inject Drivers in the boot Images Right Click on Boot Images->Properties->Windows PE tab.
To Categories the Drivers Click on Drivers->Select the drivers
Click On Categories and Create your Own Category.
Boot Images:
Add Distribution Point on the Boot Images. Click on the Distribution point Tab->New Distribution Point ->Server Share as Shown in Screen Shots Below.
Note: on Successful Configuration of PXE Service Point You will see the Server share in Distribution point List
Add Operating System Images
1) Right Click on Operating System Image ->Add Operating System Image->Select the Path for the WIM File.
2) On Successful addition of Image Close the Tab
3) Add Distribution Point to the OS package.
Create Package for SCCM 2007 Client
Add Distribution Point to the package
Create Task Sequence
In the Configuration Manager console, navigate to System Center Configuration Manager / Site Database / Computer Management / Operating System Deployment / Task Sequences.
2. On the Create a New Task Sequence page, select Install an existing image package, and then click Next.
3. On the Task Sequence Information page, specify a descriptive name for the task sequence, and then add an optional comment. To specify the boot image that will be associated with the task sequence, click Browse to open the Select a Boot Image dialog box, select the boot image, and then click Next.
The boot image you specify must match the chip architecture installed on the target computer or the task sequence will fail
4. On the Install the Windows Operating System page, specify the operating system image package that will be used to install the operating system image by clicking Browse button to open the Select an Operating System Image dialog box, and then selecting the operating system package. If there are multiple images in the image package, use the drop-down to specify the Image that should be associated with the image package. To partition and format the target computer's hard disk prior to installing the operating system image, select the Partition and format the target computer before installing the operating system check box.
Do not select the option to format and partition the target computer hard disk if you have saved the user state locally or the saved user state will be erased. For more information about saving the user state locally on the target computer for in-place operating system deployment scenarios
5. Additionally, on the Install the Windows Operating System page, specify the Windows Product key and Server license mode. If you specify Per server as the license mode, configure the Maximum server connections that will be permitted. You must also specify if the local administrator account should be disabled, or if you want to use the same administrator account for all target computers by selecting the appropriate option. Click Next
6. On the Configure the Network page, specify if the target computer will join a Workgroup or a Windows Domain.
7. On the Install ConfigMgr page, click the Browse button to launch the Select a Package dialog box and select the Configuration Manager 2007 package that will be used to install the Configuration Manager 2007 client. Specify the Configuration Manager 2007 client installation properties that will be used in the Installation properties window. Click Next
8. On the Include Updates in Image page specify how the target computer will install assigned software updates by selecting the appropriate option.
9. On the Install Software Packages page, click New to open the Program Select dialog box. Click Browse to open the Select a Package dialog box. Select the Configuration Manager 2007 packages you want to include, and then click OK. Use the drop down to select the associated programs that should be used.
10. To complete the creation of the new task sequence, on the Summary page, click Next.
To partition the hard drive select the partition disk tab in the task sequence. Select the highlighted section
Give partition name and partition type. And press OK
Task Sequence Advertisement
Right Click on Task Sequence and click on advertise
Please Tick on Make this Task Sequence Available to Boot Media and PXE
Click on Next to Finish
To verify the Advertisement click on the collection->Properties and select Advertisement Tab
Boot the Computer from Network
Great Link (Y)!!!
This is a good starter post, but how about adding some capability pertaining to computer naming and general customizations.
Example: How to integrate Windows System Image Manager. Its seems pretty straight forward, but in my testing, my unattend.xml file parameters are not taking affect.
Thanks, very good guide!
after installation, what will be the windows 7 user account and password?
excellent guide - thanks for creating!
Hi Zied
Thanks for your comment. You can set up Admin Password in Task Sequence.
Thanks Dan...
When adding the distribution point, why do you select the SCCM server and not the SCCM\PXEIMAGE?
can u email me OSD docs plz
I have been hitting a blank wall on this until I saw your post. I'm going to follow it and run some tests. Nice work! I have been wondering about the relationship between WDS and SCCM and I think you started me on the right path.
very helpful link, thanks for posting it !!!


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Prior to release, inmates will return all of the items issued at the time of booking. Inmates can be charged for any missing items. Release times will vary. If the inmate has a balance on their inmate account they will be returned in the form of cash up to $5.00. If there is an excess of $5.00 a debit card will be issued. If inmates have less than $2.00 in their account at the time of release a RTD bus ticket is available upon request. Inmates are allowed one local phone call upon release.
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We track four people on their journey.
A look at the history of France's black community
26 May 2010 09:26 GMT
She also said "the international community has a responsibility and the duty to respond" to the sinking.
Al Jazeera's Tony Birtley, reporting from Seoul, said Clinton's three to four hour stopover was to show US solidarity with South Korea and to brief officials there on what China - where Clinton had just visited - was saying about the situation.
On Tuesday, Pyongyang said "from now on it will put into force the resolute measures to totally freeze the inter-Korean relations, totally abrogate the agreement on non-aggression between the North and the South and completely halt the inter-Korean co-operation," according to the official Korean Central News Agency (KCNA).
However, despite its angry rhetoric North Korean authorities allowed South Korean workers to enter the jointly-run Kaesong industrial park that is a lucrative source of income for the North.
It also did not cut military communication lines which are necessary for the Kaesong operations, Seoul's unification ministry said.
A spokesman for the ministry said the North had approved the entry of South Korean workers to the Kaesong industrial park, where companies from the South have factories employing some 40,000 low-cost North Korean workers.
The move to let in workers suggested that behind its tough stance, the isolated North was being careful not to take steps that will undermine its already tattered economy.
Seoul's financial markets, battered the previous day partly due to rising tensions on the divided peninsula, looked stable in early trading on Wednesday.
Tuesday's comments from the North marked a new high in tensions on the divided peninsula after the March sinking of the South Korean warship, Cheonan, which Seoul blamed on a torpedo fired by the North.
Pyongyang has said the accusation was tantamount to a declaration of war.
Referring to the South's government as "military gangsters, seized by fever for a war", North Korea said if the South crossed into its side of the disputed sea border - the scene of deadly clashes in the past - the North would "put into force practical military measures to defend its waters".
On Monday, South Korea's president cut all trade with the North and blocked its commercial ships from sailing through the South's waters.
He also said he planned to take the Cheonan sinking to the UN Security Council and vowed that the South would forcefully defend itself should the North show aggression again.
A report by international investigators last week accused the North, already under international pressure over its nuclear programme, of torpedoing the Cheonan corvette on March 26, killing 46 sailors.
But the North accused South Korea's government of falsely blaming Pyongyang for the sinking, partly to help the ruling party in local elections due to be held next week.
'Appropriate' response
On Tuesday Hillary Clinton, the US secretary of state, said that Washington and Beijing would work together to come up with an "effective, appropriate" response to the sinking.
She said both sides should examine the issue over time, suggesting quick UN Security Council action was unlikely.
"[China] shares with us the goal of a denuclearised Korean Peninsula and a period of careful consideration in order to determine the best way forward in dealing with North Korea," she said.
But China, the North's only major ally who in effect bankrolls its economy, has studiously tried to keep out of the fray, urging calm and refusing to voice support for the international report on the Cheonan sinking.
On Wednesday, China's foreign ministry repeated calls for calm and restraint, with Zhang Zhijun, the vice-foreign minister, saying China had no first-hand information on the sinking.
"We have always believed that dialogue is better than confrontation," Zhang said.
In Washington, a spokesman for the state department called Pyongyang's approach "odd".
"I can't imagine a step that is less in the long term interest of the North Korean people than cutting off further ties with South Korea," PJ Crowley said.
The US has said it "fully supports" South Korea's move to impose sanctions on the North, calling it "entirely appropriate".
And the White House has directed the US military to work closely with South Korea "to ensure readiness and to deter future aggression".
US and Japanese defence chiefs also discussed the issue at the Pentagon on Tuesday with Robert Gates, the US defence secretary, thanked his Japanese counterpart Toshimi Kitazawa for Japan's strong response to the Cheonan sinking.
A Japanese defence official told the Reuters news agency that the two discussed "future North Korean possible activity" during the 40-minute meeting.
The Pentagon said US, Japanese and South Korean defence chiefs would hold trilateral talks in Singapore next month on the sidelines of a security conference.
Most analysts doubt either side would deliberately risk a war, but say there is a serious risk that small skirmishes along the heavily-defended border could turn into broader conflict.
Some worry pushing Kim Jong-il, the North Korean leader, too far may leave him little choice but to fight back to try to save his family's more than 60-year hold over the destitute country as he tries to secure the leadership succession for his youngest son.
Source: Al Jazeera and agencies
Joint military exercises announced as inter-Korean tension mounts over sinking of warship.
Seoul steps up war of words amid heightened tensions over sinking of navy ship.
Transport apps are revolutionising commutes for the middle classes, but poorer citizens still face daily gridlock.
Poverty & Development, Asia, India
Cubans get amnesty at the US border, but reaching it involves a risky journey along the Central American migrant trail.
Human Rights, Latin America, Cuba
In many ways, Michael is just like any other 11-year-old, but he has had four open heart surgeries.
Health, US & Canada
Follow a dancer through the exhaustion and jubilation of Peru's days-long Virgin of Candelaria festival.
Arts & Culture, Latin America, Peru
We travel to Chile to meet a group of young scientists determined to develop smart plant-based food to save the planet.
Science & Technology, Chile, Food
We follow the journey of a plastic bottle to find out what it takes to truly recycle the product creating zero waste.
Science & Technology, US & Canada, Environment
A couple working to protect LA communities from gang violence is caught in the crossfire of the US war on gangs.
Politics, Gang violence, US
South Koreans drink twice as much liquor as Russians, but is this national obsession with alcohol reversible?
Health, Asia, South Korea


text:
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text:
|Publication number
|Publication type
|Application number
|Publication date
|Apr 24, 1979
|Filing date
|Mar 16, 1977
|Priority date
|Mar 16, 1976
|Also published as
|Publication number
|Jiri Janata
|Original Assignee
|Imperial Chemical Industries Limited
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (2), Non-Patent Citations (1), Referenced by (24), Classifications (31)
|External Links: USPTO, USPTO Assignment, Espacenet
This invention relates to organic compositions, and the preparation and use thereof, and to devices comprising such compositions.
German OLS No. DT 25 41 308 Al describes a membrane comprising a hydrophobic polymer substrate having pendant from it essentially hydrocarbon chains carrying groups specifically reactive with a chemical compound, particularly a polypeptide. The aforementioned patent specification describes in particular the preparation of such a membrane by contacting a membrane having pendant hydrocarbon chains with a compound having appropriate reactive sites enabling it to bind the hydrocarbon to the specifically reactive group. Particularly exemplified therein is the use of a non-ionic material such as epichlorohydrin or a bis-epoxide as the binding compound.
The present invention provides a modification of the invention described in the aforementioned patent application in which, instead of a non-ionic compound we employ an ionic material to bind the specifically reactive group to the hydrocarbon chain.
Accordingly the present invention provides a specifically reactive composition comprising a hydrophobic organic polymeric substrate having pendant therefrom essentially hydrocarbon chains, said chains carrying groups specifically reactive with a chemical compound, and said groups being attached to the said hydrocarbon chains via an ionic grouping.
In a preferred composition according to the invention the linkage is an ionic protein binding linkage capable of binding a protein to the hydrocarbon chain of the composition. Any suitable ionic compound may be employed, the essential requirement being that it is capable of binding to at least a proportion of the pendant hydrocarbon chains of the composition and forming a linkage with the specifically reactive group and that where it forms a linkage with a protein it does not, under the conditions employed or to which the composition is exposed, cause undesirable denaturation of the protein.
Such materials have been described for example as mild cross-linking agents in protein chemistry (obviously most relevant when a protein component of the composition is contemplated.)
Our preferred ionic linkage is derived from an imido ester of the formula: ##STR1## where R is an alkyl group, conveniently a lower alkyl, e.g. methyl or ethyl, and preferably methyl, and X is HCl or HBr, preferably HCl.
A hydrocarbon chain containing such a linkage may thus be represented as the imido ester thus: ##STR2## where R' is a hydrocarbon chain, which may be saturated or unsaturated, and which may be branched or straight. R' will usually contain from 6 to 20 carbon atoms, although it may contain more carbon atoms particularly if it is branched unsaturated. Preferably it will contain 8 to 14 carbon atoms. The hydrocarbon will usually be aliphatic.
Preferred is the methyl dodecyl imido ester.
The composition of the invention may find many applications where linkage of a grouping to a hydrophobic substrate is desired, for example in chromatography or selective reaction systems, and the compositions of the membranes of the aforementioned German OLS may find similar application also.
However, the composition finds use particularly in the form of a membrane which may be employed in association with a suitable electrical system, for example an electrode system, for analysis, detection or concentration determination. Thus in a preferred embodiment of the invention there is provided a hydrophobic membrane having protein immobilising ability, in association, e.g. encapsulating an electrode, conveniently a highly conductive electrode for example platinum. If a protein e.g. an antibody, having a selectivity for reaction with a particular protein (antigen) is reacted at the protein-reactive site, immersion of such a coated electrode in association with a reference electrode into an aqueous solution containing the antigen provides an electrically sensitive system capable of measuring the change in electrical charge of the solution-polymer interface caused by the capture of a particular protein (antigen) by the electrode with an immunoreactive antibody. Alternatively, an antigen may be immobilised on the membrane to selectively capture a corresponding antibody.
Thus, according to a further aspect of the invention there is provided an electrode encapsulated within a sheath of specifically reactive membrane as described above.
According to a further aspect of the invention there is provided a method of detecting the presence, and optimally the concentration, of a compound in a mixture containing the compound together with other molecules, e.g. solvent molecules, by contacting the mixture with an electrode encased within a membrane as described above specifically reactive with the compound, and detecting any resulting change in the electrical charge on the membrane. Conveniently this may be accomplished by comparison with an appropriate reference electrode.
A hydrophobic polymeric membrane having selective immunochemical ability (i.e. a specifically reactive membrane) is prepared by forming a membrane of a hydrophobic polymer. The polymer is preferably one which is capable of being swollen with an organic solvent, or mixture of solvents, particularly aliphatic solvents. Particularly useful polymers in the practice of this invention are those hydrophobic polymers which contain no pendant polar groups. Typical polymers for this purpose include thermoplastic polymers such as polyvinyl chloride, polystyrene, polyethylene, polypropylene, silicone rubber, polyurethane, polycarbonate, polytetrafluoroethylene and the like. Thermosetting polymers such as epoxy resins and crosslinked polyesters may also be used. Preferred polymers are those which may be coated upon an electrode by dip-casting or shrink-fitting.
The polymeric membrane is then treated with a solvent system capable of swelling the membrane for a period sufficient to result in swelling of the membrane at least to an extent that the hydrocarbon chain may be incorporated into the membrane in sufficient concentration, as subsequently described. The solvent system contains, besides an appropriate solvent, a hydrocarbon compound having a reactive site thereon, preferably at or near one end of the hydrocarbon chain. The solvents used to swell the polymeric membrane are preferably those which may be readily removed by drying of the polymer. Thus, lower molecular weight solvents are generally preferred to higher molecular weight solvents. As indicated hereinafter, it is preferred that the solvent is of a lower boiling point and more easily evaporated than the hydrocarbon compound having a reactive site thereon. A typical solvent mixture for PVC comprises petroleum ether of a 30� to 60� C. boiling range and toluene. Other solvents or mixtures thereof may be employed for the swelling of PVC or other polymeric materials, but these will be known to the skilled man or they may be determined by simple tests.
After the polymeric membrane has been soaked in the solvent system for a period sufficiently long to effect the required degree of swelling, the membrane is dried at an appropriate temperature to remove the solvent without removing substantial quantities of the hydrocarbon compound having the reactive site. When petroleum ether, toluene and solvents of similar boiling point range are utilised a typical drying temperature is about 50� to 100� C., conveniently 50� to 60� C., preferably under vacuum. Removal of the solvent gives a membrane having hydrocarbon chains pendant therefrom, each of said chains having a reactive group thereon. Selection of the solvent (and thereby its boiling point) and pressures to be employed in its removal will be made with regard to the nature and properties of membrane and of the hydrocarbon. The polymers used herein have known solvents for swelling same.
Following attachment of the hydrocarbon chain to the polymeric membrane a series of treatments may follow to provide a specifically reactive membrane, i.e., a membrane having the ability to capture a specific compound, for example a protein, an enzyme, or a mono- or poly-saccharide, as in an immunochemical reaction.
It will be appreciated that the hydrocarbon chain may be provided with the specific reactive group before its introduction into the membrane (in which case it will of course be necessary to ensure that the group is free to display its reactivity--preferably by ensuring that it is not located less than about 4-6 carbon atoms from the membrane), or the group may be introduced into the chain after its attachment to the membrane.
We prefer to provide the hydrocarbon chain with its protein reactive linkage before incorporation into the polymer and the incorporation of an imido ester into the polymer and subsequent contact with a proteinaceous specific reactive group may be represented schematically as follows: ##STR3##
Where the hydrocarbon component is branched and of sufficient size incorporation into the polymer may occur at more than one location, i.e. two or more branches may form attachments to the polymer. When a branched hydrocarbon is employed branching will preferably occur at one or more carbon atom other than the α carbon so that the functioning of the ester link is not unduly interfered with.
It is desirable that there is no likelihood of cross-linking between a pair of pendant groups pending from the membrane surface.
After the protein-reactive linking compound (i.e. compound containing an immobilising group) is reacted with the chain pending from the membrane surface to produce the protein linking grouping, it is preferably washed and placed in a solution containing the protein to be immobilised. A preferred reaction temperature is room temperature and it is generally preferred to allow one or two days for the reaction to proceed. The reaction is generally conducted in a slightly basic medium.
After the protein is attached, it is treated to wash off residue of unreacted materials and further reacted with a compound to neutralise any unreacted protein-reactive, i.e. immobilising, groups which remained after reaction with the protein molecule.
In conducting the process according to the instant invention, it is generally preferred to use a hydrocarbon compound which has a sufficient chain length to permit the reactive group to be somewhat remote from the surface of the membrane. Generally the hydrocarbon compound employed according to the invention has at least six carbon atoms in the main chain length. A preferred length is one in which the main chain of the hydrocarbon compound contains about eight to fourteen carbon atoms, and in any case is preferably selected so that the specific reactive group is distant from the membrane surface by at least 4, and preferably at least 6 or even 8 carbon atoms. If a protein-reactive linking compound which is reacted therewith is of significant chain length, so that the ultimate protein-reactive group is pendant from the surface by at least six carbon atoms, then the chain length of the hydrocarbon need not be as long.
The novel membranes of the instant invention are particularly useful inasmuch as they can be utilised in devices for detecting the presence of a particular compound qualitatively and, preferably, quantitatively in a given mixture e.g. solution containing the compound.
A sheath of the hydrophobic polymeric membrane containing a hydrocarbon chain with a reactive group is formed on the measuring electrode (the `immunoelectrode`) in a thickness of about 10 to 50 microns, with a thickness of about 20 to 40 microns being particularly preferred. The membrane preferably does not exceed 100μ, nor is less than 5μ in thickness. A protein or other appropriate compound of an immunochemical pair is immobilised in the membrane. The measuring electrode is used in conjunction with a reference electrode. The two electrodes are immersed in a mixture, typically a solution which contains a protein or other compound of the type sought to be identified. The measuring electrode and the reference electrode are electrically connected to a meter sensitive to very slight changes in electrical potential. As the particular protein is captured by the measuring electrode, the electrical potential at the polymer-solution interface changes. The slight change is detected by the meter, which has a high impedence in electrode circuitry, thus indicating the presence of the compound. By calibration, the meter may be used to determine quantitatively the amount of compound present in the solution.
The reference electrode may be of any of a wide range of convenient electrode materials many of which are known, although we have found it advantageous to employ as the reference electrode a second immunoelectrode, substantially identical with the measuring electrode except that the specific reactive sites are blocked with a suitably reactive blocking agent so that the reference electrode is no longer responsive to the particular material being tested for. It does, however respond to non-selective adsorption of other molecules in the test solution as does the measuring electrode, so that the effect of non-selective adsorption, where it occurs, may be compensated for; measurement of the potential of an active measuring immunoelectrode against the potential of an identical (reference) immunoelectrode with blocked binding sites can effectively eliminate the effect of non-specific interactions.
Compositions and particularly membranes according to the invention also find application as preparative or reaction aids, facilitating immobilisation of one or more reactants with the potential of reversible immobilisation for example by changing the polarity of the membrane by passing an electric current through it.
The invention is illustrated by the following example.
5.0 ml of dodecyl nitrile was dissolved in 5.0 ml of dry methanol and 50 ml of dry ether contained in a flask fitted with a condenser. The mixture was cooled in an ice-bath and dry HCl slowly bubbled through it for 1 hour. A drying tube was attached at the end of the condenser. The flask was then sealed and held for 24 hours at 0� C. Ether was then evaporated under vacuum (at 30� C.) until the ester crystallised as white solid. This was washed with dry ether three times. The dry solid was stored at +4� C. in a desiccator.
PVC film is pre-swelled in dry toluene for 5 minutes and then placed in mixture of methanol:toluene:petrol ether (30-60) (1:2:1) containing 3% of the imidoester. After the three hour period the film is placed in a vacuum desiccator for 1 hour after which time the excess of the imidoester is washed off with dry methanol. Any traces of the swelling mixture are then removed in a vacuum drying pistol at room temperature for 12 hours. The film is then left in the solution containing 5% of protein to be immobilised (pH 8.4) for 48 hours at room temperature and at the end of this period washed in buffer/physiological saline solution.
This procedure can be used as a general procedure for immobilisation of proteins on hydrophobic polymers.
|Cited Patent
|Filing date
|Publication date
|Sep 16, 1974
|Jun 29, 1976
|The University Of Utah
|Novel protein-immobilizing hydrophobic polymeric membrane, process for producing same and apparatus employing same
|Sep 10, 1974
|Dec 28, 1976
|Palo Alto Medical Research Foundation
|Separation of proteins by hydrophobic adsorption
|Peter D. G. Dean, Enzyme Technology Digest, vol. 3, No. 1, pp. 2-22, (1974).
|Citing Patent
|Filing date
|Publication date
|Apr 16, 1979
|Nov 11, 1980
|Technicon Instruments Corporation
|Electrode for voltammetric immunoassay
|Mar 6, 1980
|Sep 1, 1981
|National Research Development, Corp.
|Ion-selective electrode and method of making said electrode
|Oct 6, 1980
|Mar 23, 1982
|Matsushita Electric Industrial Co., Ltd.
|Coenzyme immobilized electrode
|Nov 13, 1981
|Mar 15, 1983
|Matsushita Electric Industrial Co., Ltd.
|Coenzyme immobilized electrode
|Oct 21, 1981
|Sep 6, 1983
|University Of Delaware
|Antibody-selective membrane electrodes
|Jan 12, 1984
|Dec 25, 1984
|Molecular Devices Corporation
|Lipid membrane electroanalytical elements and method of analysis therewith
|Jan 15, 1982
|Jan 7, 1986
|Terumo Corporation
|Selectively permeable film and ion sensor
|Nov 22, 1983
|Jun 3, 1986
|The United States Of America As Represented By The United States Department Of Energy
|Field emission chemical sensor for receptor/binder, such as antigen/antibody
|Mar 8, 1983
|Oct 28, 1986
|Ajinomoto Company Incorporated
|Chemically modified electrodes and their uses
|Apr 12, 1985
|Feb 17, 1987
|Ionics, Incorporated
|Structure selective fibrous sorbents and method and apparatus for using the same
|Jan 14, 1985
|Dec 15, 1987
|Sensor Diagnostics, Inc.
|Measurement of ligand/anti-ligand interactions using bulk conductance
|Nov 19, 1987
|Jul 12, 1988
|Markwell Medical Institute, Inc.
|Biological fluid measuring device
|Jan 4, 1983
|May 9, 1989
|Rolf Siegel
|Materials and method for immobilizing biologically active substances
|May 20, 1988
|Apr 24, 1990
|General Electric Company
|Electrical detection of the immune reaction
|Jan 31, 1989
|May 22, 1990
|Board Of Regents, The University Of Texas
|Methods and apparatus using galvanic immunoelectrodes
|Sep 2, 1988
|Nov 27, 1990
|Sri International
|Immobilized valinomycin molecule for K+ sensor
|Jul 7, 1988
|Feb 19, 1991
|Markwell Medical Institute, Inc.
|Biological fluid measuring device
|May 30, 1997
|Nov 9, 1999
|Board Of Trustees, Leland Stanford, Jr. University
|Hybrid biosensors
|Jul 9, 1998
|Apr 18, 2000
|Board Of Trustees, Leland Stanford, Jr., University
|Hybrid biosensors
|Jan 15, 1997
|Jul 17, 2001
|Research Corporation Technologies, Inc.
|Peptides for altering osteoblast adhesion
|Jun 7, 1995
|May 21, 2002
|Aventis Pharmaceuticals Products, Inc.
|Activated and conjugated polystyrene substrate
|Nov 30, 1999
|Jun 24, 2003
|The United States Of America As Represented By The Department Of Health And Human Services
|Amperometric biomimetic enzyme sensors based on modified cyclodextrin as electrocatalysts
|Oct 16, 1989
|May 3, 1990
|Sepracor Inc
|Process for the covalent surface modification of hydrophobic polymers and articles made therefrom
|Jul 31, 2014
|Feb 26, 2015
|Yamamoto-Ms Co., Ltd.
|Plating apparatus and sensing device using same
|U.S. Classification
|205/775, 204/403.06, 204/418, 204/296, 435/817, 435/181, 204/403.1, 205/792
|International Classification
|Cooperative Classification
|European Classification


text:
Review Details
SA Sports Fever Recurve MAGNUM 175 lb Crossbow with Cocking Rope 543
Product Review (submitted on November 1, 2012):
Good Bow, would buy again, Everthing came timely, and in good working order. Really takes 2 to load the string, but the starter string made it easy to get it done. Buy more arrows, ditch the aluminum and get carbon. Scope is fine for this bow. Overall quite satisfied!


text:
Stress does not help with weight loss. It could even make it harder. So instead of letting all that tension or anxiety build up, find ways of releasing stress through relaxation or meditation. Put some music on, close your eyes and clear your mind.
OverdriveOnline.com strives to maintain an open forum for reader opinions. Click here to read our comment policy.


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Political Space : Frontiers of Change and Governance in a Globalizing World
AUTHOR: Yale H. Ferguson, R. J. Barry Jones
EDITION: First Edition
PUBLISHER: State University of New York Press
Also available at Amazon.com
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text:
Willie Nelson's Sentence: Sing "Blue Eyes" In Court
Readers will recall that Willie Nelson, red bandana and all, was stopped last November and arrested on charges of marijuana possession. We wrote a little something about it. Let's try to forget that arresting Willie on possession charges of what officially amounts to under three ounces is ridiculous.
What's even more ludicrous about this case, now, is that the prosecutor, one C.R. "Kit" Bramblett, has offered Nelson and his lawyers a plea deal of appearing in court personally, paying a small fine - and performing "Blue Eyes Crying In The Rain" in the courtroom.
This is a clear case of favoritism and fandomonium, irresponsible, and should be decried as unacceptable. Hopefully, the fact that the County Judge, the honorable Becky Dean-Walker, has stated that her court is "not a jester court" will ensure that this circus move never unfolds.
It shouldn't have been proposed in the first place, however. This cowpoke move by Bramblett, the County Attorney for Hudspeth Coutnty, comes as a result of the possession charge being adjusted from the initial six ounces to less than three ounces.
That places the case back into the jurisdiction of this court, something that Bramblett has jokingly attributed to an instance of he and the Hudspeth County sheriff "[throwing] out enough of it or smok[ing] enough so that there's only three ounces, which is within my jurisdiction."
Second is the absolutely unfair arrangement that Bramblett proposes. While he has claimed that Dean-Walker "wants to meet Willie," it seems that Bramblett is the one trying to secure a ditty from the Red Headed Stranger. There's no way in hell that the court would allow or consider asking a rapper like Snoop Dogg or Devin The Dude, both well-known advocates of the green themselves, to perform a song in the courtroom as the extent of their "community service."
Neither would a court allow any normal person to perform the duties of his profession in the court in order to escape the charges and secure a deferred sentence. It's not like they'll be asking the next CPA to do taxes for the judge or having someone landscape the courthouse lawn as their act of community service.
The fact remains that the charges against Nelson carry a maximum 180 days in jail and/or $2,000 fine, and that common practice in these cases does not require the defendant to appear in court, but normally resolves such matters through the mail. So just let Willie quietly pay his fine, and maybe issue a private apology for arresting him in the first place, and let the whole thing fade away instead of churning it into a spectacle.
Besides, Mr. "Kit" Bramblett, what makes you think you've got any right to demand that the legendary Willie Nelson play the specific song you've selected?
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I wish someone had told me earlier on how fucking awesome it is to watch your kids grow up. Sure, people mentioned what a joy it is to teach them new things and how sitting around eyeing the baby take its first wobbly step made their uterus get all knotted up around their throat because OH MY! THE KID DOESN'T REALLY BELIEVE HE'S A KITTEN. HE CAN WALK ON TWO LEGS DAMMIT…..!! OR AT LEAST THINKS HE'S AN EVOLVED SPECIES OF SUPER CAT AND THAT MAKES HIM A FUCKING GENIUS!!!
No. What someone should have told me is how much of a delight it is when your children begin talking like a real adult. But not a very intelligent adult. More like a mentally challenged senior with wet brain. So you get to hear funny shit all day long and laugh to yourself at their expense.
Because even though toddlers say humorous things, they don't always understand why mommy thinks the phrase, "I don't want to put that bell pecker in my mouth! It's yucky looking…. I'll get the cooties!!!", is funny.
But just wait until they hit their mid-forties early twenties… That phrase will suddenly take on a whole new meaning. I'm pretty certain I remember uttering it at the drive-in theater to a hopeful young lad when I was in high school.
The girl was the winner today. Because I really didn't have a come-back for the bell pecker comment. This mama has seen some pretty unappealing peckers in her day. And I really didn't want to be responsible for forcing the girl to put a potentially cootie-coverd pecker in her mouth against her will. There's plenty of time for that later… After all, isn't that what college is for?
((In case you're wondering, semantics won the battle and we ate cookies.))


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I'd respect her; just as I would respect a man who can keep his mouth shut when he doesn't know what he is talking about.
Best Answer
Encourage her to speak up more since conversation is an organic process and evolves as it progresses.
Best Answer
This is like a dream
Best Answer
Well, you either sugar code stuff keep your mouth shut. Its better than complete BS coming out of her mouth.
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I would say she's a wise girl. :)
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Almost everything at Crocodile Park in the southern Philippines is centered around the sharp-toothed reptile – even the ice cream. Read More »
Apr 4, 2014
Economy & Business
Economy & Business
Almost everything at Crocodile Park in the southern Philippines is centered around the sharp-toothed reptile – even the ice cream. Read More »
A Thai crocodile farmer is donating meat to help feed victims of a district in Thailand that has been overwhelmed by flooding.
This week, Sri Ayutthaya Crocodile Farm began giving 50 kilograms of crocodile tail meat to feed flood victims in the Rua district of Ayutthaya province, which is located 70 miles north of Bangkok.
Fifty-nine out of 84 villages, or 70% of the district, have been submerged under water as deep as 1.8 meters. With more water expected to be released from a major dam upstream soon, flood-hit residents will likely remain at one of the nine shelters or live on their houses' upper floors for some time. Read More »
Scientists call it involuntary repopulation. In layman's terms, the floods that have inundated large tracts of Thailand in recent weeks are enabling hundreds of crocodiles to break free from their pens at rural "skin farms" and try to make new lives for themselves in the wild.
It's a frightening complication to the country's flood crisis, which has claimed nearly 270 people since late July. Local television footage has shown images of crocodiles basking on front porches as flood-waters race by while the owners of some crocodiles have put up a sizable bounty for the safe return of their animals. Read More »
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The good, the bad and the so-so policy moves and economic swings that impacted Indonesia this week.
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All that burning rubbish in Indonesia may be taking its toll, with nearly a quarter of people surveyed in a recent poll saying waste management was the most prominent environmental issue in the country.
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