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Breastfeeding and Working: You Can Do It
Breastfeeding comes with many health benefits for both you and your baby. The American Academy of Pediatrics (AAP) recognizes human milk as the preferred nutrition source for infants. For women who choose to breastfeed, doing so may prove more difficult when it is time to go back to work. Returning to work outside the home during the first year after birth can create barriers to breastfeeding and may cause women to stop nursing when they return to work.
Why Breastfeed After Returning to Work?Studies have found evidence that breastfeeding decreases your child's risk of ear infections, lung infections, diarrhea, obesity, sudden infant death syndrome, and many other conditions. For these reasons, the AAP now recommends breastfeeding for the first year of a child's life. Breastfeeding also benefits mom by reducing the risk of postpartum depression, speeding the return to pre-pregnancy weight, and possibly reducing the risk of several serious diseases including ovarian and breast cancers, high blood pressure, diabetes, and heart disease. Breastfeeding also provides health benefits to infant. Since breastfed infants tend to be sick less often, working mothers who breastfeed avoid lost days at work. By breastfeeding, you will also strengthen the mother-infant bond, which will help boost your confidence. A woman's confidence in herself as a mother may be vulnerable when she becomes separated for her infant for long periods of time. Continuing to breastfeed serves as a constant reminder of the mother-infant bond despite the pressures a new mother may encounter during the workday. Finally, mothers may find breastfeeding convenient, since they avoid the preparation and expense that formula feeding requires.
The ChallengesThere are several challenges you may encounter when you return to work as a breastfeeding mom. Working mothers who breastfeed usually have concerns about producing enough milk for their babies. Long work hours and stress can decrease their milk supply. In addition, working moms who breastfeed may be more fatigued and may need to plan their time more carefully than bottle-feeding moms.Other challenges faced by breastfeeding moms at work include finding time and/or space to breastfeed their baby (or pump breast milk) during the workday, or an overall lack of support at the workplace. How can you overcome these potential barriers and make a smooth transition to becoming a breastfeeding mom who works outside the home?
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Definitions for vivency
This page provides all possible meanings and translations of the word vivency
Webster Dictionary
manner of supporting or continuing life or vegetation
Origin: [L. vivens, p. pr. of vivere to live.]
Translations for vivency
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- 350Promote
Throw Pillow
Emergedby Agnes-cecile
Throw Pillow made from 100% spun polyester poplin fabric, a stylish statement that will liven up any room. Individually cut and sewn by hand, each pillow features a double-sided print and is finished with a concealed zipper for ease of care. Sold with or without faux down pillow insert.
Amazing work! :)
Loving it Agnes. Respect ;-)
Great detail, beautiful piece!
beautiful work!
wow beautiful!
beautiful work
Wonderful work!
Beautiful color composition countered with very nice detailed illustration. Really nice work.
wow! :)
Pastel rainbow, amazing
Loving the watercoloring! Keep up the amazing work!
Gorgeous work
Why is it that when I'm out walking the dog late at night, the only things that ever 'emerge' from the trees or lake are big and scary and have lots of teeth :) Really nice. Really like.
So beautiful!
This piece is just stunning, love it!
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text: US Census Census Data: Fewer People Living In Poverty In N.H. By Associated Press • Sep 18, 2014 The U.S. Census Bureau is releasing new data that shows about 17,000 fewer people were living in poverty in New Hampshire in 2013 compared to the year before. The 1.3 percent decline from about 128,500 people in poverty in 2012 to roughly 111,500 last year is the second sharpest drop in the nation, trailing only Wyoming's 1.7 percent decrease. In New Hampshire, 8.7 percent of the population lives in poverty, compared with 15.8 percent nationally. The national rate in 2012 was 15.9 percent. N.H. Population Grew Slowly Last Year By Ryan Lessard • Jan 3, 2014 The U.S. Census Bureau estimates New Hampshire's population grew by only .1% last year. That's fewer than 2000 people added since 2012. That's among the slowest rates of growth in the nation. Listen Listening... / 1:08 Minority Birth Rates In NH Don't Match National Trends By Sarah Ashworth • May 17, 2012 For the first time in U.S. history, more babies are being born to minority parents than to non-Hispanic white parents. Thirty Years Of Low Child And Senior Poverty In NH By Amanda Loder • Feb 24, 2012 We're all about cool maps at StateImpact, and we just couldn't resist sharing this one on the changing face of child and senior poverty over the past 30 years. Demographer Kenneth Johnson at the Carsey Institute recently crunched some 2010 Census data, and working with a team at


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Are you Kunihiro Hongo?Claim your profile
Publications (30)96.48 Total impact
Article: Structural basis of Cu, Zn-superoxide dismutase amyloid fibril formation involves interaction of multiple peptide core regions[Show abstract] [Hide abstract]
ABSTRACT: Cu, Zn-superoxide dismutase (SOD1), an enzyme implicated in the progression of familial amyotrophic lateral sclerosis (fALS), forms amyloid fibrils under certain experimental conditions. As part of our efforts to understand ALS pathogenesis, in this study we found that reduction of the intramolecular disulfide bond destabilized the tertiary structure of metal free wild-type SOD1 and greatly enhanced fibril formation in vitro. We also identified fibril core peptides that are resistant to protease digestion by using mass-spectroscopy and Edman degradation analyses. Three regions dispersed throughout the sequence were detected as fibril core sequences of SOD1. Interestingly, by using three synthetic peptides that correspond to these identified regions, we determined that each region was capable of fibril formation, either alone or in a mixture containing multiple peptides. It was also revealed that by reducing the disulfide bond and causing a decrease in the structural stability, the amyloid fibril formation of a familial mutant SOD1 G93A was accelerated even under physiological conditions. These results demonstrate that by destabilizing the structure of SOD1 by removing metal ions and breaking the intramolecular disulfide bridge, multiple fibril-forming core regions are exposed, which then interact with each another and form amyloid fibrils under physiological conditions. © The Authors 2015. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.
Article: Anthocyanin suppresses the toxicity of Aβ deposits through diversion of molecular forms in in vitro and in vivo models of Alzheimer's disease[Show abstract] [Hide abstract]
ABSTRACT: Objectives The pathogenesis of Alzheimer's disease (AD) is strongly correlated with the aggregation and deposition of the amyloid beta (Aβ1-42) peptide in fibrillar form, and many studies have shown that plant-derived polyphenols are capable of attenuating AD progression in various disease models. In this study, we set out to correlate the effects of anthocyanoside extracts (Vaccinium myrtillus anthocyanoside (VMA)) obtained from bilberry on the in vitro progression of Aβ fibril formation with the in vivo effects of this compound on AD pathogenesis. Methods Thioflavin T fluorescence assays and atomic force microscopy were used to monitor Aβ amyloid formation in in vitro assays. Effects of Aβ amyloids on cellular viability were assayed using cultured Neuro2a cells. Cognitive effects were probed using mice that simultaneously expressed mutant human Aβ precursor and mutant presenilin-2. Results Addition of VMA inhibited the in vitro formation of Aβ peptide fibrils and also reduced the toxicity of these aggregates toward Neuro2a cells. A diet containing 1% VMA prevented the cognitive degeneration in AD mice. Curiously, this diet-derived retention of cognitive ability was not accompanied by a reduction in aggregate deposition in brains; rather, an increase in insoluble deposits was observed compared with mice raised on a control diet. Discussion The paradoxical increase in insoluble deposits caused by VMA suggests that these polyphenols divert Aβ aggregation to an alternate, non-toxic form. This finding underscores the complex effects that polyphenol compounds may exert on amyloid deposition in vivo.
Article: Bilberry Anthocyanins Neutralize the Cytotoxicity of Co-Chaperonin GroES Fibrillation Intermediates[Show abstract] [Hide abstract]
ABSTRACT: The co-chaperonin GroES (Hsp10) works with chaperonin GroEL (Hsp60) to facilitate the folding reactions of various substrate proteins. Upon forming a specific disordered state in guanidine hydrochloride, GroES is able to self-assemble into amyloid fibrils similar to those observed in various neurodegenerative diseases. GroES therefore is a suitable model system to understand the mechanism of the amyloid fibril formation. Here, we determined the cytotoxicity of intermediate GroES species formed during fibrillation. We found that neuronal cell death was provoked by soluble intermediate aggregates of GroES, rather than mature fibrils. The data suggest that amyloid fibril formation and its associated toxicity toward cell might be an inherent property of proteins irrespective of their correlation with specific diseases. Furthermore, in the presence of anthocyanins that are abundant in bilberry, we could inhibit both fibril formation and the toxicity of intermediates. Addition of bilberry anthocyanins dissolved the toxic intermediates and fibrils, and the toxicity of the intermediates was thus neutralized. Our results suggest that anthocyanins may display a general and potent inhibitory effect on the amyloid fibril formation of various conformational disease-causing proteins.
Article: Role of C-terminal negative charges and tyrosine residues in fibril formation of α-synuclein[Show abstract] [Hide abstract]
ABSTRACT: α-Synuclein (140 amino acids), one of the causative proteins of Parkinson's disease, forms amyloid fibrils in brain neuronal cells. In order to further explore the contributions of the C-terminal region of α-synuclein in fibril formation and also to understand the overall mechanism of fibril formation, we reduced the number of negatively charged residues in the C-terminal region using mutagenesis. Mutants with negative charges deleted displayed accelerated fibril formation compared with wild-type α-synuclein, demonstrating that negative charges located in the C-terminal region of α-synuclein modulate fibril formation. Additionally, when tyrosine residues located at position 125, 133, and 136 in the C-terminal region were changed to alanine residue(s), we found that all mutants containing the Tyr136Ala mutation showed delays in fibril formation compared with wild type. Mutation of Tyr136 to various amino acids revealed that aromatic residues located at this position act favorably toward fibril formation. In mutants where charge neutralization and tyrosine substitution were combined, we found that these two factors influence fibril formation in complex fashion. These findings highlight the importance of negative charges and aromatic side chains in the C-terminal region of α-synuclein in fibril formation.
Article: Suppression of Sup35 amyloid fibril formation by group II chaperonin from Thermoplasma acidophilum
Article: Varied effects of Pyrococcus furiosus prefoldin and P. furiosus chaperonin on the refolding reactions of substrate proteins[Show abstract] [Hide abstract]
ABSTRACT: Prefoldin is a molecular chaperone found in the archaeal and eukaryotic cytosol. Prefoldin can stabilize tentatively nascent polypeptide chains or non-native forms of mainly cytoskeletal proteins, which are subsequently delivered to group II chaperonin to accomplish their precise folding. However, the detailed mechanism is not well known, especially with regard to endogenous substrate proteins. Here, we report the effects of Pyrococcus furiosus prefoldin (PfuPFD) on the refolding reactions of Pyrococcus furiosus citrate synthase (PfuCS) and Aequorea enhanced green fluorescence protein (GFPuv) in the presence or absence of Pyrococcus furiosus chaperonin (PfuCPN). We confirmed that both PfuPFD and PfuCPN interacted with PfuCS and GFPuv refolding intermediates. However, the interactions between chaperone and substrate were different for each case, as was the final effect on the refolding reaction. Effects on the refolding reaction varied from passive effects such as ATP-dependent binding and release (PfuCPN towards GFPuv) and binding which leads to folding arrest (PfuPFD towards GFPuv), to active effects such as net increase in thermal stability (PfuCPN towards PfuCS) to an active improvement in refolding yield (PfuPFD towards PfuCS). We postulate that differences in molecular interactions between substrate and chaperone lead to these differences in chaperoning effects.
- [Show abstract] [Hide abstract]
ABSTRACT: The Escherichia coli chaperonin GroEL subunit consists of three domains linked via two hinge regions, and each domain is responsible for a specific role in the functional mechanism. Here, we have used circular permutation to study the structural and functional characteristics of the GroEL subunit. Three soluble, partially active mutants with polypeptide ends relocated into various positions of the apical domain of GroEL were isolated and studied. The basic functional hallmarks of GroEL (ATPase and chaperoning activities) were retained in all three mutants. Certain functional characteristics, such as basal ATPase activity and ATPase inhibition by the cochaperonin GroES, differed in the mutants while at the same time, the ability to facilitate the refolding of rhodanese was roughly equal. Stopped-flow fluorescence experiments using a fluorescent variant of the circularly permuted GroEL CP376 revealed that a specific kinetic transition that reflects movements of the apical domain was missing in this mutant. This mutant also displayed several characteristics that suggested that the apical domains were behaving in an uncoordinated fashion. The loss of apical domain coordination and a concomitant decrease in functional ability highlights the importance of certain conformational signals that are relayed through domain interlinks in GroEL. We propose that circular permutation is a very versatile tool to probe chaperonin structure and function.
Article: Covalent structural changes in unfolded GroES that lead to amyloid fibril formation detected by NMR: Insight into intrinsically disordered proteins[Show abstract] [Hide abstract]
ABSTRACT: Co-chaperonin GroES from Escherichia coli works with chaperonin GroEL to mediate the folding reactions of various proteins. However, under specific conditions, i.e. the completely disordered state in guanidine hydrochloride, this molecular chaperone forms amyloid fibrils similar to those observed in various neurodegenerative diseases. Thus, this is a good model system to understand the amyloid fibril formation mechanism of intrinsically disordered proteins. Here, we identified a critical intermediate of GroES in the early stages of this fibril formation using NMR and mass spectroscopy measurements. A covalent rearrangement of the polypeptide bond at Asn45-Gly46 and/or Asn51-Gly52 that eventually yield β-aspartic acids via deamidation of asparagine was observed to precede fibril formation. Mutation of these asparagines to alanines resulted in delayed nucleus formation. Our results indicate that peptide bond rearrangement at Asn-Gly enhances the formation of GroES amyloid fibrils. The finding provides a novel insight into the structural process of amyloid fibril formation from a disordered state, which may be applicable to intrinsically disordered proteins in general.
Article: Covalent Structural Changes in Unfolded GroES That Lead to Amyloid Fibril Formation Detected by NMR[Show abstract] [Hide abstract]
ABSTRACT: Co-chaperonin GroES from Escherichia coli works with chaperonin GroEL to mediate the folding reactions of various proteins. However, under specific conditions, i.e. the completely disordered state in guanidine hydrochloride, this molecular chaperone forms amyloid fibrils similar to those observed in various neurodegenerative diseases. Thus, this is a good model system to understand the amyloid fibril formation mechanism of intrinsically disordered proteins. Here, we identified a critical intermediate of GroES in the early stages of this fibril formation using NMR and mass spectroscopy measurements. A covalent rearrangement of the polypeptide bond at Asn45-Gly46 and/or Asn51-Gly52 that eventually yield β-aspartic acids via deamidation of asparagine was observed to precede fibril formation. Mutation of these asparagines to alanines resulted in delayed nucleus formation. Our results indicate that peptide bond rearrangement at Asn-Gly enhances the formation of GroES amyloid fibrils. The finding provides a novel insight into the structural process of amyloid fibril formation from a disordered state, which may be applicable to intrinsically disordered proteins in general.
Article: Isolation of short peptide fragments from α-synuclein fibril core identifies a residue important for fibril nucleation: A possible implication for diagnostic applications[Show abstract] [Hide abstract]
ABSTRACT: alpha-Synuclein is one of the causative proteins of the neurodegenerative disorder, Parkinson's disease. Deposits of alpha-synuclein called Lewy bodies are a hallmark of this disorder, which is implicated in its progression. In order to understand the mechanism of amyloid fibril formation of alpha-synuclein in more detail, in this study we have isolated a specific, ~20 residue peptide region of the alpha-synuclein fibril core, using a combination of Edman degradation and mass-spectroscopy analyses of protease-resistant samples. Starting from this core peptide sequence, we then synthesized a series of peptides that undergo aggregation and fibril formation under similar conditions. Interestingly, in a derivative peptide where a crucial phenylalanine residue was changed to a glycine, the ability to initiate spontaneous fibril formation was abolished, while the ability to extend from preexisting fibril seeds was conserved. This fibril extension occurred irrespective of the source of the initial fibril seed, as demonstrated in experiments using fibril seeds of insulin, lysozyme, and GroES. This interesting ability suggests that this peptide might form the basis for a possible diagnostic tool useful in detecting the presence of various fibrillogenic factors.
Article: A Potentially Versatile Nucleotide Hydrolysis Activity of Group II Chaperonin Monomers from Thermoplasma acidophilum[Show abstract] [Hide abstract]
ABSTRACT: Compared to the group I chaperonins such as Escherichia coli GroEL, which facilitate protein folding, many aspects of the functional mechanism of archaeal group II chaperonins are still unclear. Here, we show that monomeric forms of archaeal group II chaperonin alpha and beta from Thermoplasma acidophilum may be purified stably and that these monomers display a strong AMPase activity in the presence of divalent ions, especially Co(2+) ion, in addition to ATPase and ADPase activities. Furthermore, other nucleoside phosphates (guanosine, cytidine, uridine, and inosine phosphates) in addition to adenine nucleotides were hydrolyzed. From analyses of the products of hydrolysis using HPLC, it was revealed that the monomeric chaperonin successively hydrolyzed the phosphoanhydride and phosphoester bonds of ATP in the order of gamma to alpha. This activity was strongly suppressed by point mutation of specific essential aspartic acid residues. Although these archaeal monomeric chaperonins did not alter the refolding of MDH, their novel versatile nucleotide hydrolysis activity might fulfill a new function. Western blot experiments demonstrated that the monomeric chaperonin subunits were also present in lysed cell extracts of T. acidophilum, and partially purified native monomer displayed Co(2+)-dependent AMPase activity.
Article: Gly192 at hinge 2 site in the chaperonin GroEL plays a pivotal role in the dynamic apical domain movement that leads to GroES binding and efficient encapsulation of substrate proteins[Show abstract] [Hide abstract]
ABSTRACT: The subunit structure of chaperonin GroEL is divided into three domains; the apical domain, the intermediate domain, and the equatorial domain. Each domain has a specific role in the chaperonin mechanism. The 'hinge 2' site of GroEL contains three glycine residues, Gly192, Gly374, and Gly375, connecting the apical domain and the intermediate domain. In this study, to understand the importance of the hinge 2 amino acid residues in chaperonin function, we substituted each of these three glycine residues to tryptophan. The GroEL mutants G374W and G375W were functionally similar to wild-type GroEL. However, GroEL G192W showed a significant decrease in the ability to assist the refolding of stringent substrate proteins. Interestingly, from biochemical assays and characterization using surface plasmon resonance analysis, we found that GroEL G192W was capable of binding GroES even in the absence of ATP to form a very stable GroEL–GroES complex, which could not be dissociated even upon addition of ATP. Electron micrographs showed that GroEL G192W intrinsically formed an asymmetric double ring structure with one ring locked in the 'open' conformation, and it is postulated that GroES binds to this open ring in the absence of ATP. Trans-binding of both substrate protein and GroES was observed for this binary complex, but simultaneous binding of both substrate and GroES (a mechanism that ensures substrate encapsulation) was impaired. We postulate that alteration of Gly192 severely compromises an essential movement that allows efficient encapsulation of unfolded protein intermediates.
Article: Identification of the Functionally Critical Amino Acid Segment and its Role in the Flexible C-Terminal Region of the Chaperonin GroEL
Article: Functional Characterization of the Recombinant Group II Chaperonin from Thermoplasma acidophilum[Show abstract] [Hide abstract]
ABSTRACT: The functional characteristics of group II chaperonins, especially those from archaea, have not been elucidated extensively. Here, we performed a detailed functional characterization of recombinant chaperonin α subunits (16-mer) (Ta-cpn α) from the thermophilic archaea Thermoplasma acidophilum as a model protein of archaeal group II chaperonins. Recombinant Ta-cpn α formed an oligomeric ring structure similar to that of native protein, and displayed an ATP hydrolysis activity (optimal temperature: 60°C) in the presence of either magnesium, manganese or cobalt ions. Ta-cpn α was able to bind refolding intermediates of Thermus MDH and GFP in the absence of ATP, and to promote the refolding of Thermus MDH at 50°C in the presence of Mg2+-, Mn2+-, or Co2+-ATP. Ta-cpn α also prevented thermal aggregation of rhodanese and luciferase at 50°C. Interestingly, Ta-cpn α in the presence of Mn2+ ion showed an increased hydrophobicity, which correlated with an increased efficiency in substrate protein binding. Our finding that Ta-cpn α chaperonin system displays folding assistance ability with ATP-dependent substrate release may provide a detailed look at the potential functional capabilities of archaeal chaperonins.
Article: Fibril Formation of hsp10 Homologue Proteins and Determination of Fibril Core Regions: Differences in Fibril Core Regions Dependent on Subtle Differences in Amino Acid Sequence[Show abstract] [Hide abstract]
ABSTRACT: Heat shock protein 10 (hsp10) is a member of the molecular chaperones and works with hsp60 in mediating various protein folding reactions. GroES is a representative protein of hsp10 from Escherichia coli. Recently, we found that GroES formed a typical amyloid fibril from a guanidine hydrochloride (Gdn-HCl) unfolded state at neutral pH. Here, we report that other hsp10 homologues, such as human hsp10 (Hhsp10), rat mitochondrial hsp10 (Rhsp10), Gp31 from T4 phage, and hsp10 from the hyperthermophilic bacteria Thermotoga maritima, also form amyloid fibrils from an unfolded state. Interestingly, whereas GroES formed fibrils from either the Gdn-HCl unfolded state (at neutral pH) or the acidic unfolded state (at pH 2.0-3.0), Hhsp10, Rhsp10, and Gp31 formed fibrils from only the acidic unfolded state. Core peptide regions of these protein fibrils were determined by proteolysis treatment followed by a combination of Edman degradation and mass spectroscopy analyses of the protease-resistant peptides. The core peptides of GroES fibrils were identical for fibrils formed from the Gdn-HCl unfolded state and those formed from the acidic unfolded state. However, a peptide with a different sequence was isolated from fibrils of Hhsp10 and Rhsp10. With the use of synthesized peptides of the determined core regions, it was also confirmed that the identified regions were capable of fibril formation. These findings suggested that GroES homologues formed typical amyloid fibrils under acidic unfolding conditions but that the fibril core structures were different, perhaps owing to differences in local amino acid sequences.
Article: Hydrophilic Residues 526KNDAAD531 in the Flexible C-terminal Region of the Chaperonin GroEL Are Critical for Substrate Protein Folding within the Central Cavity[Show abstract] [Hide abstract]
ABSTRACT: The final 23 residues in the C-terminal region of Escherichia coli GroEL are invisible in crystallographic analyses due to high flexibility. To probe the functional role of these residues in the chaperonin mechanism, we generated and characterized C-terminal truncated, double ring, and single ring mutants of GroEL. The ability to assist the refolding of substrate proteins rhodanese and malate dehydrogenase decreased suddenly when 23 amino acids were truncated, indicating that a sudden change in the environment within the central cavity had occurred. From further experiments and analyses of the hydropathy of the C-terminal region, we focused on the hydrophilicity of the sequence region (26 KNDAAD 531 and generated two GroEL mutants where these residues were changed to a neutral hydropathy sequence (526 GGGAAG 531) and a hydrophobic sequence (526 IGIAAI 531), respectively. Very interestingly, the two mutants were found to be defective in function both in vitro and in vivo. Deterioration of function was not observed in mutants where this region was replaced by a scrambled (526 NKADDA 531) or homologous (526 RQEGGE 531) sequence, indicating that the hydrophilicity of this sequence was important. These results highlight the importance of the hydrophilic nature of 526 KNDAAD 531 residues in the flexible C-terminal region for proper protein folding within the central cavity of GroEL.
Article: Mechanical unfolding of covalently linked GroES: Evidence of structural subunit intermediates[Show abstract] [Hide abstract]
ABSTRACT: It is difficult to determine the structural stability of the individual subunits or protomers of many proteins in the cell that exist in an oligomeric or complexed state. In this study, we used single-molecule force spectroscopy on seven subunits of covalently linked cochaperonin GroES (ESC7) to evaluate the structural stability of the subunit. A modified form of ESC7 was immobilized on a mica surface. The force-extension profile obtained from the mechanical unfolding of this ESC7 showed a distinctive sawtooth pattern that is typical for multimodular proteins. When analyzed according to the worm-like chain model, the contour lengths calculated from the peaks in the profile suggested that linked-GroES subunits unfold in distinct steps after the oligomeric ring structure of ESC7 is disrupted. The evidence that structured subunits of ESC7 withstand external force to some extent even after the perturbation of the oligomeric ring structure suggests that a stable monomeric intermediate is an important component of the equilibrium unfolding reaction of GroES.
Article: Structural Stability of Covalently Linked GroES Heptamer: Advantages in the Formation of Oligomeric Structure[Show abstract] [Hide abstract]
ABSTRACT: In order to understand how inter-subunit association stabilizes oligomeric proteins, a single polypeptide chain variant of heptameric co-chaperonin GroES (tandem GroES) was constructed from Escherichia coli heptameric GroES by linking consecutively the C-terminal of one subunit to the N-terminal of the adjacent subunit with a small linker peptide. The tandem GroES (ESC7) showed properties similar to wild-type GroES in structural aspects and co-chaperonin activity. In unfolding and refolding equilibrium experiments using guanidine hydrochloride (Gdn-HCl) as a denaturant at a low protein concentration (50 microg ml(-1)), ESC7 showed a two-state transition with a greater resistance toward Gdn-HCl denaturation (Cm=1.95 M) compared to wild-type GroES (Cm=1.1 M). ESC7 was found to be about 10 kcal mol(-1) more stable than the wild-type GroES heptamer at 50 microg ml(-1). Kinetic unfolding and refolding experiments of ESC7 revealed that the increased stability was mainly attributed to a slower unfolding rate. Also a transient intermediate was detected in the refolding reaction. Interestingly, at the physiological GroES concentration (>1 mg ml(-1)), the free energy of unfolding for GroES heptamer exceeded that for ESC7. These results showed that at low protein concentrations (<1 mg ml(-1)), the covalent linking of subunits contributes to the stability but also complicates the refolding kinetics. At physiological concentrations of GroES, however, the oligomeric state is energetically preferred and the advantages of covalent linkage are lost. This finding highlights a possible advantage in transitioning from multi-domain proteins to oligomeric proteins with small subunits in order to improve structural and kinetic stabilities.
Article: Multiple Structural Transitions of the GroEL Subunit Are Sensitive to Intermolecular Interactions with Cochaperonin and Refolding Polypeptide[Show abstract] [Hide abstract]
ABSTRACT: In this study we attempted to determine the specific roles of the numerous conformational changes that are observed in the bacterial chaperonin GroEL, by performing stopped-flow experiments on GroEL R231W in the presence of a refolding substrate protein. The apparent rate of one kinetic phase was decreased by ∼25% in the presence of prebound unfolded malate dehydrogenase while another phase was suppressed completely under the same conditions, reflecting different effects of the unfolded protein on multiple structural transitions within GroEL. The addition of cochaperonin GroES counteracts the effect of the bound substrate protein in the former case, but had no effect on the latter, more extensive suppression. Using a chemically modified form of GroEL R231W which is incapable of releasing substrate proteins at low temperatures, we identified a conformational transition that is implicated in the release of substrate proteins. Parts of the actual process of substrate protein release were also observed through fluorescence resonance energy transfer experiments involving GroEL and labeled substrate protein. Analysis of the energy transfer data revealed an interesting relationship between substrate protein displacement and a specific structural transition in the GroEL apical domain.
Article: A novel ATP/ADP hydrolysis activity of hyperthermostable group II chaperonin in the presence of cobalt or manganese ion. FEBS Lett[Show abstract] [Hide abstract]
ABSTRACT: A novel ATPase activity that was strongly activated in the presence of either cobalt or manganese ion was discovered in the chaperonin from hyperthermophilic Pyrococcus furiosus (Pfu-cpn). Surprisingly, a significant ADPase activity was also detected under the same conditions. A more extensive search revealed similar nucleotide hydrolysis activities in other thermostable chaperonins. Chaperonin activity, i.e., thermal stabilization and refolding of malate dehydrogenase from the guanidine-hydrochloride unfolded state were also detected for Pfu-cpn under the same conditions. We propose that the novel cobalt/manganese-dependent ATP/ADPase activity may be a common trait of various thermostable chaperonins.
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Tottori University
TTJ, Tottori, Japan
- • Department of Chemistry and Biotechnology
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text:
Carrie v3.0 by AlexPascenkoWatch
©2012-2016 AlexPascenko
so thats carrie from castlevania 64 again ,redesign, 3rd version , it´s sweet to see how i evolved from my first version i did a year ago . i think i´ll go with that one.
my name's fred, i'm a bailbondsman... I'd, i'd like to talk to carrie
Looks nothing like Carrie, quite good though.
Nice one!
The way you drew those boots look really cool.
Don't know who she is, but good work
Compared to your other ones this one retains more elements of Carrie's designs. I could buy this being a grown up Carrie. She looks a bit like a gypsy, you retained the cloak from her old outfit, but made it more ragged from the years. That helps make her identifiable, and while the hairstyle has changed I think her expression has that troubledlook that speaks of Carrie's personality.
thank you very much for that insight full comment. personally i am not too happy with her outfit. but that drawing was a good lesson to see how far and long i can spend with rendering on an outfit.
Very nice!!
very cool
really nice. i dig it all and got a chuckle from her coin purses being so close to her genital area im sure u didnt mean that though. personally i would have moved them just a bit more over to her left, but thats not important i guess. if thats the only critique i have, then this piece is super cool. i love the rendition
Not familar with the character, but this looks good, nice details.
Grown up? o.o
Hah, that was my favourite game for N64! I remember Carrie a little differently though with a couple of ribbons and definitely more shy than this. But if you think of this as a grown-up version - totally possible Very nice outfit and I love the not 100% clean pencils.
Awesome drawing!
Stellar design! Great detail work. Keep it up!
beautiful character design.
Well, this is just awesome, love it!
She's beautiful! Great work!
Nice! I love it!
just remove ze balls
omg, those little pouches look like she got balls O_o
(love her face. classic beauty!)
(love her face. classic beauty!)
i agree . special bags of testosterone make her more badass. well, thats what i hoped for. no more balls though.
REALLY REALLY AMAZING... great picture


text:
Hey BMD619
Glad to hear you're looking for a clan. I am the CoD game officer for the League of Casual Gamers. We are a tight clan of about 120 members located all across the globe and we play all kinds of games. We are currently ranked 16 but we are working hard towards getting that red tag and we would love to have you on aboard.
For a quick view you can check out our recruitment video: https://www.youtube.com/watch?v=h7s36K0cO7I
You can also check out our webpage for even more information: http://locg.enjin.com/
If you have any questions you can get in touch with me right away through PSN: jnwrotc
Hope to see you online soon mate!


text: I am looking for the opinion of an expert in economics on the Rolling Jubilee project. If you do not have any formal education in economics, I would kindly request you to please make clear that your opinion is layman speculation. Website: http://rollingjubilee.org/ Warning: layman speculation below I don't understand how this could work. How do you just erase debt? Who is losing money here? The banks? How can the banks just afford to erase the debt? Isn't this just encouraging the banks to give more bad loans?


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text: Browning Citori 12 ga 31/2" Listed in category: Guns > Shotguns > Browning Shotguns > Over Unders > Citori > Hunting /UserImages/140930/914981422 3868698.jpg Item Details Condition: Used, Minor Wear Item #: 914981422 Caliber: 12 Gauge Location: WI SKU: Have one to sell? Sell Now! Sell it yourself, Ship it yourself, or use a GA DropOff Location! Classified Ad Details This listing is no longer available or has been sold. Price: $1,000.00 Login/Register to Buy Shipping: $30.00 Payment Methods: Return Policy: See Description Seller Details (More items from MikeH9568) Name: MikeH9568 Seller Since: Feb. 2012 User Level: Basic FFL Dealer: No Positive feedback: 100% View Seller Security Verification (What's this?) This seller has completed 38 transactions Address has been verified and will be sent on request at completion of transaction Click here to request seller's GunsAmerica Verified Address Description Shipping and Payment Additional Information This 12 ga 3 ½" chamber Browning Citori has a few very minor marks in the stock and bluing from some light use but other than that is in great shape – Both barrels have been ported and the forcing cones lengthened. -- The gun has 30" barrels that have removable invector plus choke tubes – Comes with a 4 and a 6 has marked tube – Bore is bright and clean. – If you have questions or would like additional pictures call or e-mail me. -- I will ship promptly to your FFl - Shipping includes insurance - Returns accepted for 7 days but only if the item is not as described. -- Payment to be made by Certified Check or Money Order (Postal Money Orders Preferred) -- Trades Accepted: No


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Forests & Fire
Forest ecosystems are dynamic and complex. A disturbance to any part of the network can alter the balance of relationships and affect the entire ecosystem either positively or negatively. Fire is unique in that it can be either a beneficial natural process or a devastating catastrophe. For species like lodgepole pine, fire is necessary to help reduce competition and help the species release its seeds. However, climate change, drought and other conditions have caused occurrences of intense wildfire to increase, which can damage forests so badly that it takes years for them to naturally recover. Wildfire is necessary for forests, but also a threat to them, so strong policies and management are imperative to make sure wildfire is working for our forests instead of against them.
Our Strategy
American Forests partners with other organizations to protect the surviving trees on burned lands and to restore forests, educating the public and key decision makers about the importance of these trees. Over the last 10 years, roughly 26 percent of our Global ReLeaf projects have restored forests damaged by fires.
In 2007, ConocoPhillips pledged $2.8 million to fund American Forests' wildfire restoration project in this area, as part of a carbon offset settlement between the energy company and California Attorney General Jerry Brown. The ongoing Cuyamaca project has laid the groundwork to encourage forest restoration throughout the region.
At the federal level, American Forests has long advocated that decision makers in the U.S. Forest Service, the U.S. Department of the Interior and Congress should address wildfire threats and develop plans and policies for wildfire mitigation and prevention. This resulted in the establishment of the Federal Land Assistance, Management and Enhancement Act (FLAME Act) Coalition, which supported the passage of the FLAME Act that would allow the federal government to enforce funding for larger emergency wildfires without taking away from other important projects.
Take Action
Visit American Forests' Action Center to send pre-written letters to Congress and other representatives to support sound wildfire policy. Letters available now include:
- Asking Congress to support the Collaborative Forest Landscape Restoration program, which restores forests and reduces fire risk in U.S. communities.
to American Forests to support our work, like wildfire restoration projects and wildfire prevention policy action.
Forest Fire News from our Loose Leaf blog
A Tale of Two Mountains: Part One
by American Forests
By Michelle Werts
It's called the Land of Enchantment. From mountains to desert, from national forests to historic monuments, New Mexico is indeed a land of many wonders. Keeping all of its wonders healthy and intact, though, is proving a bit complicated.
Southwest of Albuquerque lies Cibola National Forest, and within Cibola loom the Zuni Mountains. Here, delicate forest restoration is at work. Wildfires i... (Read More)
Wanted: Wildfire
by American Forests
By Michelle Werts
Wildfires are one of the most prolific forces affecting our nation's forests. They are often intense and unpredictable — and are only likely to become more so in the coming decades thanks to climate change. They threaten homes and communities, which is why American Forests supports projects like the U.S. Forest Service's Collaborative Forest Landscape Restoration program, designed to reduce... (Read More)
Tree News
by American Forests
By Tacy Lambiase
Over the past week, we've been tracking several interesting stories related to forests and trees around the world. From wildfire season predictions to victories over an invasive insect species, here are several stories from the world of trees.
Arlington Cemetery Proposes Plan That Would Cut Down Almost 900 Trees


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Marcello Mastro Barebangs Kevyn Luss
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The world may be on the brink of another financial collapse, this time with the Chinese economy as the likely catalyst, and with Britain holding $500 million in Chinese debt, the "sceptered isle" may bear a major portion of the impact.
One thing is certain: The world's economy can't continue with an agenda of unlimited development and endless consumption of throwaway goods, given that limits to extractive resources may have already peaked.
British radical economist James Meadway has been tracking the state of the globalized economy, and he sees major shifts already underway as China shifts from its economic roles.
Meadway, formerly an economist with the New Economics Foundation, described his concerns in a September essay for the Guardian:
Has globalisation peaked? Two fundamental factors suggest it may have. First, the financial crisis itself revealed the systemic weaknesses inherent in an over-extended financial system. Major financial institutions, banks chief among them, are now significantly more wary about reaching beyond their home bases. In the event of a future crisis, they will require strong, supportive states ready to back them up. This has drawn banks and states closer together, with weak states and weak banks propping each other up, as in the eurozone's "sovereign-bank nexus" (the strong links between government debt and banks).
Second, states themselves are acting strategically. Globalisation was associated with a belief in the supreme merits of government inaction on the economy, but governments are increasingly strategic economic actors.
China is attempting an immense shift away from its decades-old role as low-cost exporter to the world, expanding both its domestic market, and seeking to create a new, regional trading block around the new Silk Road. The collapse of its stock market, naturally, necessitated a huge (if deeply flawed) government intervention. Protectionism is on the rise, whilst yuan devaluation has raised the spectre of "currency wars". The German state, meanwhile, is an assiduous defender of its own interests as a manufacturing exporter.
Failure to address the looming crisis will only make the crisis worse, he explains.
In this, the latest edition of Taiq Ali's Telesur English series, The World Today, Meadway explains his concerns as well as possible reforms to adjust the world's economy to the new realities of the 21st Century:
The World Today: The State of the Economy
Program notes:
Tariq Ali talks to James Meadway, radical economist, about the global economy, the failure of world leaders to effectively resolve the financial crisis in 2008, and the probability of another crisis occurring in the future.


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Run run Mirko by EltasiaWatch
©2012-2016 Eltasia
Male Amur Tiger | Mirko
Shot through glass
★ Location: ZOO Sauvage de Saint-Félicien [link]
Lens: Canon 70-200mm f/2.8L IS II USM
★ Copyright © Eltasia. All rights reserved
You may NOT use, replicate, manipulate, or modify this image.
Amazing capture!
Another great shot, I`d really love to see a whole excibition only with your tiger photos, they are awesome!!!
Awesome shot, beautiful. I always love it when Tigers run, rather than just lying around, it gives them that wild-vibe.
Tigers are one of the most beautiful creatures on the planet. I love these shots! :3
This is amazing and just stunning. Wow.


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Ondawa Greens is a hilly, scenic course that owners Steve and Sue Greene are continually improving on. The 9 hole executive length course is fun for every generation of golfer. We welcome families, teams and […]
Hours & Pricing
Hours Ondawa Greens is open daily from sun up to sun down, 7 days a week. We open as soon as the weather clears in the spring and stay open daily until the snow threatens to […]
Pro Shop
Located in the big barn is the Pro Shop where various clubs and other golf accessories are for sale. Broke a club or need new grips? Leave them at the Pro Shop where Steve will […]
Come all for Cheese!
For those who missed it Tracy will again be cutting the Cheese!! Something not to be missed!!!
WE ARE OFFICIALLY OPEN Saturday April 11, 2015
Wednesday Night League 2015-2016 will start April 29th….Sign up or email me if you are interested.
Wednesday Flag Tournament
If it is not thunder and lightening plan on playing…but bring your umbrella…Pray for sun!!
Longest Drive AM
Jen Burk and Joel Millard AM
In 2005 Steven and Susan Greene, native Washington County residents purchased a Golf Course and Driving Range in desperate need of TLC. The first few years were tough as there was no equipment, running water, nor electricity. Each season Steven and Susan strive to make some form of improvement either to the course or structure so their patron's would have a better golfing experience. Within the last two years a beautiful Club House and Pro Shop were added to offer amenities to golfers to make their golfing experience more memorable. Steven and Susan are committed to making improvements at Ondawa Greens, each year and encourage their clients to keep coming back to see what improvement they have made!
Steven and Susan, graduates of Greenwich Central School, reside in Jackson, NY with their golden retriever Lily and cat No. 2. Family is important to the Greene's business philosophy with two daughters both graduates of Salem Central School, Rebecca '09 and Cassandra '12. They are committed to cultivating a culture that highlights Washington County's beautiful landscape while offering a memorable family experience. So whether you are a seasoned golfer or just starting out, Ondawa Greens offers terrain to meet all levels of golf.


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CHARITY: Chief Executive Sarah Lindsell, Chairman of the Board Andy Foote and Jane Hutt
/ News
TO welcome new support services for brain tumour patients and their families in Wales, Assembly Member Jane Hutt hosted an event at the Senedd.
The Brain Tumour Charity funds research and works to raise awareness and offer support and information, services now being provided throughout Wales.
Jane Hutt said: "I was delighted to be able to show my support for the charity's work and to meet some of those whose lives have been touched by brain tumours."
Michelle Herbert, operations manager for The Brain Tumour Charity in Wales, said: "The key thing for patients and families affected by a brain tumour is that we are here for them, wherever they are in Wales and whatever the support they need.
"We are providing services throughout Wales, from a base in Wales."
Promoted Stories
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"National Geographic's 'The 10 Best Chocolatiers in the World' " Katrina Markoff named, "THE innovator in chocolate to lead the US through the next 30 years" byFood & Wine Magazine
Vosges Haut-Chocolat's intriguing chocolate creations are made with the finest ingredients from around the world, sourced by Founder and Chocolatier Katrina Markoff. Katrina's fusion of indigenous spices, flowers, roots, herbs and liqueurs with premium chocolate creates a sensory experience that nurtures awareness of and appreciation for the world's diverse cultures.
Vosges Haut-Chocolat harnesses the power of storytelling to open minds, spirits and palates through esteemed chocolate, inspiring us all to interact with the world in a new and creative way. With 14 years of chocolate innovation, Vosges Haut-Chocolat offers an array of exotic delicacies from truffles, marshmallows, and caramels, to chocolate bars, and many seasonal offerings.
Katrina is the recipient of numerous accolades including Fortune Magazine's 40 under 40, the Bon Appétit Food Artisan of the Year Award, and being named "THE innovator in chocolate to lead the US through the next 30 years" by Food & Wine Magazine. She was also honored as Woman Entrepreneur of the Year by OPEN American Express and Entrepreneur Magazine. Most recently, Vosges Haut-Chocolat was named one of the 10 Best Chocolatiers in the World by National Geographic.
Vosges Haut-Chocolat's latest endeavor is the construction of a Platinum LEED-certified green manufacturing and chocolate lifestyle house in Chicago, another step in expanding Katrina's mission of spreading Peace, Love, and Chocolate®.
Explore an array of chocolate selections and find a boutique near you at www.vosgeschocolate.com
This 6 inch cube is brimming with a myriad of chocolates – 16pc Exotic Truffle Collection, ½ lb Bapchi's Caramel Toffee, 3oz Super Dark Coconut Ash & Banana Bar, 3oz Super Dark Pomegranate & Goji Bar, 3oz Reishi Mushroom & Walnut Bar, 4pc Blush Caramels, 4pc Peanut Butter Bonbons.
An array of haut-chocolat, offering something to assuage every chocolate desire. Includes 9 Exotic Truffles, 4 Caramel Marshmallows, 4 Peanut Butter Bonbons, 8 Exotic Caramels, ¼ lb Bapchi's Caramel Toffee.
Exclusive 16pc Golden Truffle Collection, featuring Katrina's handpicked favorite parfums, ½ lb Bapchi's Caramel Toffee, 9pc Caramel Marshmallow.
Katrina's handpicked favorites!
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Valentine declarations
Warnings/notes : Seto/Joey, slightly weird, slight fluff, slight hints at Yami/Yugi and Bakura/Ryou.
Disclaimer : I don't own Yu-Gi-Oh.
written at 14th november 2003, by Misura
"Kaiba, you may be a bit of a bastard, but I still have this strange wish for you to kiss me. And since today's Valentine and we only live once, I was wondering if maybe you'd oblige me," Joey sincerely told his bathroom-mirror, which obliged by making him look quite handsome, even if he did say so himself.
He sighed, hoping it would be enough. Writing declarations of love wasn't something he considered himself to be very talented at. In fact, this was his first attempt at one. Still, Kaiba probably hadn't received that many either, considering the way he tended to treat people.
In fact, maybe it would be better to scrap the 'bit of a' part in front of 'bastard'. It would certainly be closer to the truth, even if it didn't sound too nice. But then again, Yugi tried to be nice to Kaiba all the time, and it wasn't as if the CEO seemed to like him any better for the effort.
Yugi ... Joey chuckled, remembering the frantic phone-call he had received from Yami the previous evening. It appeared Yugi had 'forgotten' to explain the special significance of the fourteenth of february to his yami, which had resulted in Yami not having a clue. Until he had met Bakura, that was, on a shopping-spree to make sure Ryou was never going to forget this year's Valentine.
Bakura being Bakura had of course immediately inquired about Yami's plans for Yugi, ready to criticize and ridicule them, only to find out that Yami had none. Which, in a way, was even better. The grand, wise Pharaoh being fully ignorant about such a widely known holiday, tsk, tsk.
It was fortunate there had still been a mall to shop in after the ensuing argument. And even more fortunate that Yugi had just happened to walk by and asked what the yamis were fighting about, so that Bakura could make vague hints and smirk, while Yami evaded the question and tried to distract Yugi from pursuing the matter further, for fear of ruining the surprise.
Yugi and Ryou were lucky, Joey mused. At least they knew they were loved back. Bakura might not be the easiest person in the world to live with, but judging by the blissful expression on Ryou's face whenever he spoke about his yami, he definitely cared about Ryou. Even if he showed so little of it in public.
"Joey! You're going to be late for school!" His father's voice interrupted Joey's musings, bringing his thoughts back to the present. Valentine's day. Grabbing the corrected speech, he read it one more time.
"Kaiba, you may be a bastard, but I still have this strange wish for you to kiss me. And since today's Valentine and we only live once, I was wondering if maybe you'd oblige me." His reflection stared back at him goofily, like an idiot. Ah well, he'd just hope for the best. Kaiba'd probably kill him regardless of his expression, simply for making the statement.
By the end of this day, he'd either have been kissed by the person he loved, or he'd be dead and never have to worry about Kaiba returning his feelings ever again. A win-win situation, in other words.
[meanwhile, elsewhere]
"Joey Wheeler, you are a mutt and a lousy duelist but I am deeply in love with you. I would like you to consider my invitation to have dinner with me tonight, so that we may get to know each other a little better and ... This is utter nonsense." Seto glared at Mokuba, who shrugged.
"You might put a little more enthusiasm in it," Mokuba suggested.
"That's not the problem," Seto shook his head. "The problem is this stupid speech! How can you even *think* this would work? It's no use."
"Well, I admit that you might have written a better one, since you're the one in love and all," Mokuba snapped, slightly peeved, "but no, you had to complete your project last night."
"It's not that I'm not grateful for your effort to help me in this - " Seto began quickly, not wanting to start the day with an argument.
"All right, then," Mokuba grinned. "Try it once more, with feeling this time. Oh, and you got the first part of it wrong. My text said 'Joey Wheeler, you are a handsome guy and a pretty good duelist' not what you made of it."
"But he's *not* a good duelist! He - " Seto protested, cut off by an impatient gesture from Mokuba.
"He got in the finals of two big tournaments, so he's not that bad. Plus, it'll warm him up to you if you flatter him a bit. Consider it a little white lie for the greater good." Mokuba smirked. "Does this mean you *do* agree that he's handsome?"
"No. The 'pretty good duelist'-part just irked me more," Seto grumbled.
"Suuuuure." Mokuba rolled his eyes. "I so believe that, big brother."
"I don't have time for this." Seto stared pointedly at the clock. Mokuba was unimpressed.
"Valentine's only once a year, so you have to grab this opportunity and make the best of it."
Yugi looked slightly panicked, Joey noted as he walked in the classroom, not late for once. Yami was nowhere in sight, as usual at school. After being explained the benefits and the drawbacks of school in the present day, both Yami and Bakura had quickly declared the knowledge they had acquired from their hikaris was enough for their needs.
That Ryou was missing as well *was* unusual, since the quiet, soft-spoken boy was usually one of the first ones to arrive. Perhaps Bakura's Valentine-celebration had something to do with it. Maybe he ought to give Ryou a call later, just to ask if everything was all right.
"Have you seen Kaiba this morning?" Joey inquired, putting his mind back to his own plans for Valentine.
"He arrived pretty early, with a scowl on his face that was even worse than his usual one," Anzu supplied, sourly holding a big teddy-bear in her arms. Joey wondered whose it was.
Yugi smiled. "Maybe he just didn't sleep very well, because he's nervous."
"Kaiba being nervous?" Joey chuckled, shaking his head incredulously. "That's hard to imagine."
"Perhaps because you simply have no imagination to mention, mutt," Kaiba sneered, brushing past without looking at either him or Yugi and Anzu.
"Speaking of the devil ... " Anzu murmured.
"Well, at least you know where he is now," Yugi remarked.
~to be concluded in the second part?~


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Switch Showtimes and Tickets
Read Movie Reviews about Switch Write a Movie Review for Switch
Release Date: 2010-08-20
Genre: Romance, Comedy
Rating: PG-13
Duration: 1hr 40min
Also available in: 3D
An unmarried 40-year-old woman turns to a turkey baster in order to become pregnant. Seven years later, she reunites with her best friend, who has been living with a secret: he replaced her preferred sperm sample with his own.
Cast and Crew
- Starring
Jennifer Aniston, Jeff Goldblum, Juliette Lewis, Patrick Wilson, Jason Bateman
- Directed by Josh Gordon, Will Speck
- Produced by Bona Fide Productions
- Written by Allan Loeb, Jeffrey Eugenides
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The Blog
Report: White House Upset With Joe Biden
10:55 AM, May 10, 2012 • By MARK HEMINGWAY
The president has already admitted Biden 'got out a little bit over his skis,' on the issue. But Politico reports that the White House is fuming that Biden's errant remarks in support of gay marriage forced the president's hand. Not only that, Biden's getting the credit for the president's change of heart when he had argued behind the scenes to hold off on making the decision for fear of alienating key segments of the electorate:
Who could have forseen that Joe Biden's legendary verbal incontinence would eventually prove to be a political liability for Barack Obama?
The Weekly Standard Archives
Browse 20 Years of the Weekly Standard


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The Nikon D7000 is the first new mid-level Nikon DSLR camera that features full 1080p HD video with full-time autofocus. This full-color guide is packed with expert advice and full-color photos to both educate and inspire as you get better acquainted with your Nikon D7000. The handy trim size allows you to take it with you wherever you go so you can frequently refer to the top-notch tips, tricks, and techniques for getting unique and memorable photos and handling problematic shooting situations. Practical examples take you beyond the basics of the functions of each button and lavish photos exemplify the professional-level photographs that you can achieve.
Nikon D7000 Digital Field Guide goes beyond the owner's manual and is as an essential sidekick when using your D7000!
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Photo : San José Atoyatenco
A picture of 'San José Atoyatenco', this photo is taken in the destination of Santa Ines Tecuexcomac in the state of Tlaxcala in the country of Mexico. You can click on the image below to see a higher resolution version of this picture, or on the links below that to see more photos by this photographer. This picture is displayed according to Panaramio Copyright Policies.
Photographer : mral23 [17 December 2012]
Photos provided by Panoramio are under the copyright of their owners


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Humpty Dumpty?
Proverbs 27:2 gives us very wise words of advice,
Let another man praise you, and not your own mouth.
There are so many people, including Christians, who think God cannot get along without them. They think they are the reason they experience so much success.
It has been said that a man wrapped up in himself makes a mighty small package.
When God grants you success and blesses you, you have to remain humble if you are going to retain your usefulness to God. The Scripture says pride goes before destruction and a haughty spirit before a fall.
There are plenty of men and women who at one time or another were on top, but today are on the bottom. I know examples inside and outside ministry. One story in particular comes to mind when I think of this principle.
There was a minister who once said, while making a plea for money, "No one is doing what we're doing throughout the world, and God needs us to carry on this work. The world cannot be reached without our ministry."
Granted, he was doing an incredible work in a lot of different countries. But the moment he said, "God can't get along without me," I thought of Humpty Dumpty. I just thought, "Oh, no! Oh, no! Oh, no!" I knew he was headed for a fall!
And you know what? That man is no longer in the position of prominence that he once was. And God seems to have gotten along fine without him.
Do not sing your own praises. Let others congratulate you if they will, but at the end of the day offer those praises to the One who really deserves it—the Lord.
Visit the Answers with Bayless Conley website for more ways to Connect with God
click here to view today's Answers with Bayless Conley broadcast at LightSource.com.
You don't have to fully understand the things that happen to know God is good. Even in your most dire hardships and pain, He knows your frustrations and He promises to show Himself good in your life! You can learn to deepen your trust in God's goodness during your storms when you listen to Lessons from My Journey – the very first message Bayless Conley gave after undergoing months of healing and rehabilitation after a near-fatal boating accident. This resource is our gift to thank you for your gift to help bring a living Jesus to our dying world.


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N.E. Tailgate: Patriots-Bengals preview
N.E Tailgate: Patriots-Bengals preview
Another week, and another test upcoming for the New England Patriots' defense.
After facing Atlanta's high-powered offense last week, the Patriots will now set their eyes on stopping Andy Dalton, A.J. Green and the rest of the Bengals.
Glenn Ordway, Fred Smerlas, and Steve DeOssie previewed the week five match against 2-2 Cincinnati on New England Tailgate.
"You can bet that they are going to do a lot with [Aqib] Talib on [Green]," DeOssie said. "They are going to do some of that over-under coverage they did on Julio Jones, where they have a cornerback underneath. He's also a guy, just like Julio Jones, he can go up and catch a pass that sometimes defensive backs can't reach."
Dalton will lead the Bengals from under center, and even though he has never thrown an interception in the red zone in his career, he is seen more as a game manager than a game changer.
"He doesn't have the intangibles," Smerlas said. "He doesn't anticipate the breaking of receivers a lot of times. He doesn't make dynamic throws. He plays it really safe, that's why they don't have a lot of interceptions in the red zone. But when you are a quarterback sometimes you have to be a gun-slinger, and he is not. He is not a great quarterback. He is in the middle of the pack."


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Who's in the Room: How Great Leaders Structure and Manage the Teams Around Them
By Bob Frisch
Is your company run by a team with no name?
At the top of every organization chart lies a myth--that a Senior Management Team makes a company's critical decisions. The reality is that critical decisions are typically made by the boss and a small group of confidants--a "team with no name"--outside of formal processes. Meanwhile, other members of the management team wonder why they weren't in the room or even consulted ahead of time. The dysfunction that results from this gap between myth and reality has led to years of unproductive team building exercises. The problems, Frisch shows, are ones of process and structure, not psychology.
In "Who's in the Room?" Bob Frisch provides a unique perspective to this widely misunderstood issue. Flying in the face of decades of organizational psychology, he argues that the solution lies not in addressing behaviors, but in unseating the senior management team as the epicenter of decision making. Using a broad portfolio of teams--large and small, permanent and temporary, formal and informal--great leaders match each decision to the appropriate team in a fluid, flexible approach that you won't find described in management textbooks.
"Who's in the Room?" is based on interviews with CEOs at organizations ranging from MasterCard to Ticketmaster to The Red Cross. Understand and embrace the way decision-making actually happens in their organizations Use these "teams with no names" to best advantage Engage the Senior Management Team in the three critical tasks for which it is ideally suited
Organizations will get better decisions and superior results by unleashing the full potential of their Senior Management Teams. And bosses will see a dramatic drop-off in people coming into their offices asking, "Why wasn't I in the room?"
EXCERPTS: Simple Sabotage: A Modern Field Manual for Detecting and Rooting Out Everyday Behaviors That Undermine Your Workplace
STAFF PICKS: Who's in the Room?
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Digital Set-Top Box Industry Growing Rapidly
Riding on the wave of growing popularity worldwide for set-top boxes which enable television viewers to enjoy the Internet, Korean set-top box makers like Samsung Electro-Mechanics, Humax and Dae Ryung are busy meeting the rising overseas demand.
A digital set-top box enables an analog television set to become a user interface to the Internet and receive and decode digital television (DTV) broadcasts.
The VCR-sized devices are classified by the broadcasting method they receive: cable TV, satellite broadcasting or Internet TV.
In the first half of this year, sales for the major domestic set-top box makers increased between 100 percent and 600 percent from the same period last year.
Set-top boxes are expected to be a great contributor to the nation's exports, along with other products like liquid crystal displays (LCD)), as the global digital broadcasting market will keep expanding rapidly for five or more years in the future.
The domestic market is still in its nascent stages, as full-fledged services for digital broadcasting will not be started until next year, and most of the set-top box makers are focusing on overseas markets.
But the domestic market's prospects are not bad, as digital broadcasting companies are expected to lend set-top boxes to subscribers free of charge for marketing purposes. Airwave and cable TV broadcasting will be digitalized in the latter half of 2001 and in 2002, respectively.
The expansion of intelligent apartments will also activate the market, as many builders will equip every household with digital set-top boxes in the future.
Industry officials expect the number of digital set-top box manufacturers to increase from the present 30 to 100 if digital broadcasting is launched.
Domestic demand for Internet TV set-top boxes is estimated at 200,000- 600,000 units this year, 1 million next year and 2 million in 2002.
The global digital set-top box market has grown rapidly since 1998 and will likely continue to do so for a few years.
The United States and Europe already launched digital cable TV services, as well as satellite broadcasting services, triggering the full-fledged production of set-top boxes for digital cable TV broadcasting. …


text:
shlomi-fish-homepage / t2 / open-source / bits.html.wml
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#include '../template.wml'
#include "toc_div.wml"
<latemp_subject "Open Source Bits and Bobs" />
<toc_div />
<define-tag bit_link whitespace="delete">

GnuPG Insta-Party Signature List Verification Script

Some key-signing parties are conducted in a way that the
a file containing the fingerprints is distributed to everybody, and is then
verified to be correct by its SHA1 hash. Then, the keyring is made available
to the public. Now the question is whether the signatures in the keyring are
the same as those in the file.
To resolve it, I wrote <a href="<bit_link "gpg-verify.pl" />">a script
that checks the signatures in the file and verifies that they are the same
as those reported by GPG in the key-ring. You can freely use it under
the terms of the MIT X11 license.
Using it I signed the keys, by first running it, and then using kgpg (part
of kdeutils) to sign all the keys at once. Truly an improvement over the
time, where I signed each key individually, using my password like a zillion

TWiki Installation Scripts

This is <a href="<bit_link "shlomif-twiki-installation-automation-2004-09-10.tar.gz" />">a collection of bash scripts I wrote to automate the installation
of TWiki - a popular Wiki implementation.

<a href="<bit_link "open-inventor-bsd-daemon/" />">Open Inventor
3-D BSD Daemon

An OpenInventor-based three-dimensional model of Beastie the BSD Daemon,
created as a Technion project in the Computer Graphics course.

<a href="<bit_link "gringotts-patch/" />">Patch for Gringotts

This is a patch for
gringotts that
allows it to compile with the latest Gtk+, and fixes some other annoyances.

<a href="<bit_link "nowplay-xchat.pl" />">X-Chat /nowplay command using XMMS

What this little Perl script does is register an
X-Chat command called <tt>/nowplay</tt>,
that whenever invoked displays a message of "/me is listening to My Artist -
My Song" in the current channel. Feel free to make any use of it whatsoever
under the terms of the MIT X11 license. It queries either
Amarok or XMMS 1.x.
You need to install the
Xmms-Perl distribution
and have the X-Chat Perl interface. To use, make it executable and put it
under your <tt>$HOME/.xchat2</tt> directory.

<a href="<bit_link "pmwiki-revert.pl" />">Revert
an (old-versioned) PMWiki Wiki to a previous state

This is a Perl script I wrote to revert a PMWiki Wiki to a previous state after
my Wiki has been spammed. Read the instructions at the top of the script. Note
that the format of the PMWiki pages has changed since the version for which
this script was intended.

<a href="<bit_link "greasemonkey/grease.html" />">Greasemonkey
Scripts for Greasemonkey.

<a href="<bit_link "linkvisitor-1.2.2.xpi" />">LinkVisitor for Mozilla Firefox 2.0.x and Below

Click on the Link to download or install the
LinkVisitor extension for Firefox
2.0.x and below. Here's
<a href="<bit_link "linkvisitor-firefox-2.0-patch.diff" />">the patch
against the CVS source.
Update: the package was modified to work on Firefox 2.0.x.

<a href="<bit_link "convert-kabc-dist-lists.pl" />">Forward Port the KAddressBook Distribution Lists to Version 3.5.4

The format used for the distribution lists has changed from earlier versions
of KDE's
KAddressBook to
the one supplied by kdepim-3.5.4. This Perl script can be used to do the porting
automatically. Read the instructions at its top. Note that it will skip
lists that have raw emails inside the distribution lists' directives. This
script is available under the MIT X11 licence, with absolutely no warranty.

Amarok Scripts

Amarok is a powerful and easy to use
music player for the KDE Desktop Environment.
Amarok supports
scripts which
allow one to customise the player using practically any language.
Here are links to the Amarok scripts I wrote:

Per-Song
Volume Script for Amarok 1.x,
Per-Song
Volume Script for Amarok 2.x - This script maintains a per-song volume
information. What it means is that a volume is associated with each song,
which is recorded whenever the volume changes, and is restored to its last
value when the song is played again. This is useful if you have several songs
which sound louder or softer than the rest.

<a href="<bit_link "qclam/" />">QClam-Shlomif

QClam is a small utility
to interface ClamAV scanning from within dot-qmail files. This is a version of
it that was improved by me to some extent.

<a href="<bit_link "sdl-flag-0.2.0.c" />">SDL
Fluttering Flag

This is a fluttering flag, not quite unlike the one presented at the
beginning of the
Future Crew's Unreal Demo, but still not as polished. It's one
self-contained ANSI C file, that requires the
SDL (Simple DirectMedia Layer) Library.
See this page for the
story of how I originally recreated the fluttering flag effect.

<a href="<bit_link "slidy-shlomif-0.2.0.js" />">JavaScript
Presentation Helper

This is a JavaScript script based on
HTML Slidy, but heavily
reduced and evolved. It supplies use of the left and right arrow keys for
moving between slides (<tt>
</tt> elements
somewhere inside an element with a class named "<tt>point</tt>"). It also
provides the "n" and "p" keys to follow the links with the appropriate
If the global variable <tt>rtl_keys</tt> is set to true, then the left and
right keys are reversed to be suitable for a language that is written
from right to left.
This file is distributed under the same terms as the original file, and I
disclaim any explicit or implicit ownership.

<a href="<bit_link "square-waves-decomposition/" />">Square Waves Decomposition of a Sine Wave

This is a program I wrote, after someone asked if it's possible to decompose a
sine wave using square waves.

du
Output Analyser

du is a UNIX command
that can recursively scan directory trees and output the space used by
them. After one has done <tt>\du . > du.txt</tt>, one can use this script
to analyse the file by filtering the sub-directories of a certain depth (and
under a certain sub-directory). (Licensed under the MIT/X11 License).

<a
href="<bit_link "xfce-set-virtual-workspaces.rb" />">Set the
Xfce Virtual Workspaces Number and Names

This command line Ruby script sets the names (and number) of the
Xfce desktop's virtual
workspaces (also known as virtual desktop. You can simply pass the names
as arguments in the command line (possibly inside quotes).
This is useful to restore them in case they were reset. Use this script while
Xfce is running. (See
a
discussion on the Xfce mailing list for more information.) Licensed
under the MIT/X11 License.
<define-tag camila_ron>
;;; Sponsored by Camila Ron
;;; Remove at 2013-10-24

#include "camila_ron.wml"
<camila_ron />


text:
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Catskills Articles
Showing 1-1 of 1 Holiday Resorts
Wolff's Maple Breeze Resort
Catskill, New York, United States
Wolff's offers great rates during the holidays of Memorial Day, Fourth of July, Labor Day, and Columbus Day. Enjoy a 4-day/3-night stay full of entertainment, fun, and relaxation. There's usually too much stress around holidays, but not when you come and enjoy them at Wolff's!... more
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text:
Hotel San Salvador - Venice
Free WiFi is featured at this hotel. Staff can provide tour/ticket assistance.
Steps away from the Rialto bridge, this hotel offered an oasis of calm in a convenient…
great location just off the Rialto Bridge...when you get to the square just look for the…
Enter dates to check prices
Hotel Info: 1-877-214-4288from $61
- Standard Double Room
- Single Room
Also popular with travelers from the USA
At a glance
Key facts
Hotel size
- This hotel has 10 rooms
- This hotel is arranged over 1 floor
- Check-in time 1 PM-11 PM
- Check-out time is 10:30 AM
Required at check in
- Credit card or cash deposit required
- Government-issued photo ID required
Travelling with others
- One child (4 years old and younger) stays free when occupying the parent or guardian's room, using existing bedding
- No cribs (infant beds)
- Pets not allowed
- Free WiFi in public areas
- Free WiFi in rooms
- No parking
Other information
- Smoke-free property
In the hotel
- Tours/ticket assistance
- Free newspapers in lobby
- Number of buildings/towers - 1
- Terrace
In the room
- Air conditioning
- Minibar
- Private bathroom
- Shower only
- Free toiletries
- Hair dryer
- Flat-screen TV
- Satellite TV channels
- Free WiFi
- Phone
- Daily housekeeping
- In-room safe
Hotel San Salvador - Venice's small print
Please note that the hotel perform a pre-authorization check on your credit card as a guarantee. This amount may correspond to the price of one night or to the total cost of your reservation.
Cash transactions at this property cannot exceed EUR 2999.99, due to national regulations. For further details, please contact the property using information in the booking confirmation.
In accordance with national/local laws, air conditioning may be restricted to certain hours from 20 September to 1 June.
This property does not have elevators.
Mandatory fees
You'll be asked to pay the following charges at check in or check out:
A tax is imposed by the city and will be collected at the property. This tax does not apply to residents of Venice City and children under 10 years of age. Further exemptions may apply, subject to submission of proper documents to the property, to patients, their companions and people staying in the city for specific purposes/duties. 30% reduction applies from 1-31 January.
- A tax is imposed by the city: EUR 2 per person, per night for adults; EUR 1 per night for guests aged 10-16 years old. This tax does not apply to children under 10 years of age.
We have included all charges provided to us by this hotel. However, charges can vary, for example, based on length of stay or the room you book.


text:
Wednesday Jan 27 2010
Hidden Falls expansion plans, pot shop ban on Placer County Planning Commission plate
By: Gus Thomson, Journal Staff Writer
Park plans and a ban on pot shops will be on the agenda at today's Placer County Planning Commission meeting. The commission is slated to consider environmental clearances and a conditional use permit that would add 979 acres to the existing 221-acre Hidden Falls Regional Park, between Auburn and Lincoln. Judy Owen, who lives adjacent to the park, said area resident concerns include the possibility of hunting on the land and potential fire dangers. Plans are to build a new access point and parking area off Garden Bar Road, expand the existing parking lot and construct a multi-use trail system on the new section of land. The commission is scheduled to start consideration of the permit and environmental impact report at 11 a.m. At 1 a.m., county Planning Division staff will be asking for a recommendation to the board of supervisors for approval of a zoning text amendment banning medical marijuana collectives, cooperatives or dispensaries in the county. The proposal would also clarify that production and composting of cannabis is not included in the definition of crop production and agricultural processing in the county code. The hearings will take place at the Planning Commission Chambers, 3091 County Center Drive, located at the corner of Bell Road and Richardson Drive in North Auburn.


text:
Problem with rear wiper
I need to remove the rear wiper motor from my 2005 Wrangler with hard top. The wiper only works sporadicly. I checked the connector at the motor and there is power coming from the switch.
How do I remove the wiper arm from the motor shaft?
I think its the same way as the front wipers. Near the base there is a retaining clip that slides back and then the arm will slide off the shaft.
Rear wiper
Great! Thanks for the help.
|All times are GMT -5. The time now is 10:19 AM.
User Alert System provided by Advanced User Tagging v3.1.0 (Pro) - vBulletin Mods & Addons Copyright © 2016 DragonByte Technologies Ltd.


text:
As unemployment has risen over the past couple of years, we've seen turnover fall to record lows. This really should come as no surprise-it's basic economics: the supply of workers increases as the demand for workers goes down. And when the demand for workers is low and there is little competition among employers for workers, wage growth is stagnant.
This, in a nutshell, is what we've just experienced. These were a few of the effects of "The Great Recession." But guess what---the recession's over. And while money may not be falling from the sky, it's important that we recognize that the tide has turned, and to quote an underappreciated British band, "It's gettin' better, man!"
Just as shifts in the economy brought about declining sales, rising unemployment, and falling turnover, as the overall economic outlook improves we are going to start seeing shifts in the other direction. Comp sales and guest counts are moving into positive territory after many months of negative sales. And since employment changes are generally a lagging economic indicator, it means workplace changes are on the horizon.
According to the recent People Report Economic Conditions Survey, 48% of restaurant companies reduced staff at the corporate level in 2009. Just 3% plan to make additional cuts in 2010. Restaurants posted positive job growth for the first three months in 2010, adding over 42,000 jobs. This was the first string of three consecutive months of job growth since the end of 2007 when the recession began.
And what's the outlook for the second quarter? More job growth ahead according to the People Report Workforce Index. Nearly all companies surveyed planned to maintain their current staffing levels or increase staff in the months ahead.
Changes to compensation may be on the horizon as well. After many companies froze salaries in 2009, the percentage of companies who have lifted this freeze has dropped considerably in 2010.
Just as we went through a period of considerable change in 2008 and 2009, we are going through a period of flux right now. It's called a recovery. If you think workplaces issues aren't a concern right now, consider this your warning. The issues you shelved for the last two years are on their way back, slowly but surely, and with an added dose of government intervention, addressing them will be even more challenging.
Topics: Human Resources
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text:
call 0844 264 2445
item - £0.00
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Browse our ready to print digital sheet music downloads OR try an advanced song search OR view the trending tags below:
We can offer UK and EU-based Music Teachers, Librarians, Orchestral Buyers and other Music Directors a 10% discount on all orders over £25.
Approval for our Educator's Account takes no more than one working day.
The educational and institutional discount is subject to Sendmemusic terms and conditions
In order to apply for the educational and institutional discount you must have a sendmemusic.com account.
Schools, Universities and Colleges will be given to the option to pay for their orders by invoice/purchase order.
You can unsubscribe anytime and we promise never to share your email address with anyone else.


text:
then and now..
☮& ♥ Becky
Proud single mommy to my 3 lucky ★'s.
"in the midst of our lives we must find the magic that makes our souls soar."
One day old
6 1/2 months:
Margie - wife to Mike and mommy to Claire Anne�born 11/24/08 and�Max Michael born 9/29/10.
Nina born 11/12/08 6 weeks early. This is her 4 days old:
Now at 6 and a half months:
❤Mom to D ('91) J ('92) D ('96) & H ('08) G-ma to K ('10) & H ('11) ❤
Kerstin wife to "N" since 1/23/4 & Mom to "L" 11/08 and "S" 08/10. MMC 2/15. Now cautiously expecting 3/16.
1 day old
2 weeks ago
wife to Chris (4/2001) and mommy to Maria (11/2008)
jmorrison: Wow! You have such a clean floor! How do you do it? My floor is the bane of my existance!
joannagum: I read what you wrote too quickly and thought it said "two weeks old" and I was like...wow...advanced child! Lol- sorry, mommy brain moment, I guess. They tried to get Faith to put her hands together sweet like that at her photo shoot, too, but she wouldn't have it.
Wife...nowamom: What a fantastic hat!!
Anyways :-) Birthday:
A few weeks ago, 6 1/2 months old:
Lover of God, my husband, and my dear daughter, born 11/10/08


text:
Blizzard is ready to go all out during the BlizzCon, showing the gaming world what the team has been working on lately. Among the games that will be in the spotlight is the first expansion for Diablo III called Reaper Of Souls.
The new trailer for the expansion which was supposed to be shown during Blizzcon has been leaked online a few hours ago, showcasing some of the new contents that will be introduced by the expansion. You can check out the trailer by going here.
Reaper Of Souls will focus on Malthael, the angel of death, who has obtained the black soulstone. The black soulstone houses the soul of Diablo himself so it's a truly dangerous artifact.
With Reaper Of Souls, Blizzard will deliver a darker tale, with Malthael acting as a true angel of death, stealing the souls from the people of Sanctuary. The expansion will be set in the city environment of Westmarch which will help making the new story feel darker than usual.
Among the new features included in Reaper Of Souls is the new Crusader class, a holy knight using shield skills as well as a new skill called the Falling Sword which turns the Crusader into a bolt of lightning to strike the enemies down. Added to the game is also a Mystic Artisan.
Diablo III: Reaper Of Souls will be released on PC sometime next year. A PS4 version of the expansion has been confirmed some time ago.


text:
Leading consulting group with a 360° approach
Providing technical leading edge innovation and specialized bridge engineering services to clients worldwide.
Specializing in the planning, design, and construction management of tunnels and underground structures.
Providing marine and waterfront engineering services to clients worldwide.
Visit our career site and meet Leona Smith, project co-ordinator from the North American COWI company, Buckland & Taylor.
2014 saw COWI achieve its best performance ever. At the same time, we worked on strengthening our high-growth areas through strategic acquisition.


text: Lely's couple make £38,000 11 January 2005Written by ATG Reporter The main talking point of Gorringes' (15% buyer's premium) December 7-8 sale in Bexhill-on-Sea was the remarkable £260,000 bid on the second day for the early 17th century pietra dura table top featured on the front page of Antiques Trade Gazette no. 1670 (25th December/1st January). But day two of the sale also yielded this impressive-looking Sir Peter Lely (1618-1680) double portrait of Sir John Rivers, 3rd Bart., and his wife Lady Anne Rivers, right, which had been bought by its vendors at Dreweatt Neate in October 1988 for £20,000. Although in reasonably good condition (albeit with an old lining) and with plenty of decorative appeal, this 4ft 71/2in x 5ft 111/4in (1.41 x 1.81m) canvas was rather too large for most people's living rooms and the sitters seemed not to have made a significant impact on English history. As a result, a West Country private buyer was able to secure it at £38,000, just under the pre-sale estimate of £40,000-60,000. Antiques Trade Gazette is the weekly bible of the fine art and antiques industry. Read articles like this every week in the Antiques Trade Gazette or ATG app. Click here to subscribe today. Back to top


text:
Safeco Auto Insurance
Since it was founded in 1923, Safeco has been a major insurer of cars and homes. Safeco – now a part of the Liberty Mutual Group and the fifth largest insurer in the country – is a company that prides itself on putting customers' needs first and pledges to works diligently to go above and beyond the call of duty.
If you're looking for a Safeco auto insurance quote, understand that you'll be able to choose yearly or six-month policies and have the benefit of around the clock claims service.
Find out more about Safeco auto insurance in your state today:
With a Safeco car insurance quote, you'll undoubtedly start with liability coverage – it's required in nearly every state. Liability coverage helps protect you if you cause a wreck resulting in injuries and property damage.
But you can customize your Safeco car insurance policy policy to fit your price range and coverage needs. Consider the following protections, some of which might be required in your state:
- Uninsured/underinsured motorist coverage. If your car is damaged in a wreck that's the fault of a driver without sufficient coverage, this facet of your policy can help pay for necessary repairs.
- Collision coverage. This comes into play if you strike something on the roadway and damage your vehicle. It can help cover repairs or replacement costs, up to your policy limit.
- Comprehensive coverage. If your car is damaged due to vandalism, theft, flood, fire, hail, or wind, this coverage can help handle the costs associated.
- Medical payments. This coverage tackles costs associated with medical needs of your passengers resulting from a wreck.
- Plus, every policyholder has the opportunity to qualify for diminishing deductibles and accident forgiveness.
For a complete rundown of coverage options and a free Safeco car insurance quote, call the number at the top of the page to speak with a licensed agent. AutoInsurance.us representatives are eager to answer any questions you may have and to find the perfect policy for you.
Finding a policy via AutoInsurance.us
By using the AutoInsurance.us service, you could get a free Safeco auto insurance quote as well as up to nine other quotes from leading carriers. All providers – including Safeco – that are represented by AutoInsurance.us have received a rating of 'Excellent' or better for financial strength by the A.M. Best Company. Armed with this knowledge, shopping for car insurance with our service will be a breeze as you can feel confidence that you're striking the right balance between coverage and cost.
Licensed agents with the AutoInsurance.us service will make sure you get every discount available as well, which can drive down your costs significantly.
Curious about how a Safeco car insurance quote stacks up against other providers? Give our representatives a call by dialing the number at the top of the page. Don't have time to call right now? No problem. You can get started online by filling in your ZIP code in the box provided.
Need information about Safeco Insurance in your state? Find it in the list below:
Make a move to Safeco
It's common knowledge that the roadways can be dangerous and that drivers are unpredictable, so it's vital to have an auto insurance policy in place before you put your keys in the ignition. It's also an inconvenience to overpay for car insurance – leaving many drivers constantly searching for the right balance between cost and coverage.
Call now, and see if a Safeco auto insurance quote is the answer, or if another provider would be a better fit. Our agents are eager to help you because we understand that you'd rather spend your hard-earned dollars on other things.


text:
Tech In-LawTwitter Stats
@techinlaw - Tracking since May 4, 2009
Bridge between old school & ubergeeks. Integrated marketer & consultant for hire. Tech In-Law on nights & weekends. Most tweets are related to sports & food.
Tech In-Law Tweets
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Tech In-Law Followers
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This account is #9,586,434 in the worldwide rank of the most popular Twitter users.
Tech In-Law Following
@techinlaw is following 1,533 Twitter accounts.
Last month this account followed 45 users.
Tech In-Law Predictions & Milestones
@techinlaw will hit 726 followers in the next 3 months, and 818 in one year.
You are on Tech In-Law's Twitter stats page
We track these Twitter stats since May 4, 2009 . You can see how many followers Tech In-Law lost or gained and what the prediction is for tomorrow or the next 15 days, together with all kinds of other stats like rank compared to all Twitter users, tweets etc.
Why don't you give it a try and compare Tech In-Law to yourself ? Or if you are Tech In-Law get even more feature rich & up-to-date stats with Premium & Pro Twitter Stats.
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text:
Ain't talkin bout jewelry when I give your bitches pearl necklaces
Lyric Meaning
He's giving nutting on deez hoes.
"Pearl Necklace" according to Urban Dictionary
The glorious culmination of tit-fucking, in which you blow your nuts out all over a girl's tits, shoulders, neck, and, with any luck, chin. one of the highest expressions of love and affection bestowable upon a woman by a man.
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