text:
- Simon's blog
- 1124 reads
So this morning in church we looked at 1 Corinthians 16, and, in what I think is probably a first when teaching on stewardship, everyone there decided to increase their giving. How did I do it?
Well, of course, having a small church helps if you want to get everyone on board. But there's another thing. I've written before about the dichotomy that comes about when people reify the church. I had a long screed here explaining this but it can basically be summed up in a picture. Most people think of Christian giving like this:
Nobody likes the idea, it causes tension, and pastors get embarrassed talking about it since they're the representatives of the receiving party, not the giving party.
The impression you get from Paul's writing, however, looks like this:
People don't give to the church, they are the church, and the church gives the money away outside of itself.
Yes, I'm aware that one interpretation of this passage is that Paul was describing one particular collection to be taken up to Jerusalem, not a general pattern of giving. I think the Περὶ δὲ construction and the use of present imperatives renders this unlikely.
I was amazed looking over this passage about how delicately Paul handles the issue. First, note that he does not stipulate how much is to be given. Then in v2 he says that giving is to be decided and set aside at home (παρ᾿ ἑαυτῷ), and so that he does not embarrass people by making a fund drive when he arrives. Next, he does not handle the church's money himself, but he asks them to arrange for trustworthy people from within the church to administer it. They are to take it directly to the recipients. Only if they ask him to will he travel with them. Everything is done and handled by the people giving the money away and there is complete transparency in how it is handled.
So we tried it. As the church planter, I tried to model Paul's role; I explained all that and a bit about the theology behind giving, and then I left the members to make a decision about what they would do. "I think we should go for it!" was the instant response. After some discussion about what to do with the money, they decided to make a weekly contribution to a charity which runs a local food bank for children's and old people's homes and local people who are struggling; one person from the church (not me, of course) would collect the contribution and take it directly to the food bank.
But the real encouragement for me was not what we decided but that, by shifting the way people think about giving, people became excited about and committed to doing more of it.


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Rose Ann Humphrey's Award Winning New England Interior Design Firm, Home Life, offers full service interior design; delivering quality and value to New England and worldwide clientele. Passionate and devoted to lifestyle, Rose Ann Humphrey has spent twenty-five years listening, sharing and helping her clients "discover their own visual voices." Home Life strives to translate feelings and need into interior design that expresses, supports and enables clients to live in their dreams.
Boston, MA and Cape and the Islands | 617.360.1008

subset: commoncrawl


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Announcing Cotton Spandex Jersey!
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We're creating a scheduling application and we need to represent someone's available schedule during the day, regardless of what time zone they are in. Taking a cue from Joda Time's Interval, which represents an interval in absolute time between two instances (start inclusive, end exclusive), we created a LocalInterval. The LocalInterval is made up of two LocalTimes (start inclusive, end exclusive), and we even made a handy class for persisting this in Hibernate.
For example, if someone is available from 1:00pm to 5:00pm, we would create:
new LocalInterval(new LocalTime(13, 0), new LocalTime(17, 0));
So far so good---until someone wants to be available from 11:00pm until midnight on some day. Since the end of an interval is exclusive, this should be easily represented as such:
new LocalInterval(new LocalTime(23, 0), new LocalTime(24, 0));
Ack! No go. This throws an exception, because LocalTime cannot hold any hour greater than 23.
This seems like a design flaw to me---Joda didn't consider that someone may want a LocalTime that represents a non-inclusive endpoint.
This is really frustrating, as it blows a hole in what was otherwise a very elegant model that we created.
What are my options---other than forking Joda and taking out the check for hour 24? (No, I don't like the option of using a dummy value---say 23:59:59---to represent 24:00.)
Update: To those who keep saying that there is no such thing as 24:00, here's a quote from ISO 8601-2004 4.2.3 Notes 2,3: "The end of one calendar day [24:00] coincides with [00:00] at the start of the next calendar day ..." and "Representations where [hh] has the value [24] are only preferred to represent the end of a time interval ...."


text:
Translation of cowslip in Spanish:
- (variedad de prímula)Example sentences
- Banks of primroses, drifts of bluebells and clusters of cowslips are now found only in secret, out-of-the-way places.
- I know a bank whereon the wild thyme blows, where oxlips and the nodding cowslip grow.
- The P. veris of Linnaeus's time is still to this day known as the cowslip.
Definition of cowslip in:
- British & World English dictionary
- US English dictionary
What do you find interesting about this word or phrase?
Comments that don't adhere to our Community Guidelines may be moderated or removed.
Grammar and usage help for Spanish:
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= trending


text:
Hi we are running a story about the celebrations of St Piran's Day in March next year in BBC Countryfile Magazine March issue. Would it be possible to get the hires of the photograph on your website of the parade zig zagging with flags up the hill. We will of course credit you.
Many thanks for your help,
Hilary Clothier
0117 3143372


text:
Thousands of people manage their DNS using EntryDNS
We are two geeks who built a free DNS service to fill a void in this space.The service is free and we hope to support it on either a freemium model or some sort of affiliate sales.
Note that we just started it, and there are still things to be done, it's still a work in progress. Nevertheless the service is at this point functional and there are people already using it.
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text:
Vets Need Help In Healing
Iraq war veteran Trevor Banks, pictured in the photo held by his brother, Malcolm, beside his mother, Felicia, could not overcome the post-traumatic stress disorder he suffered and as a result took his own life. (Photo by DN-R File)
To See This and Every Local Story from The Daily News-Record, Click Here to Become a Subscriber!
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text:
FISH & WILDLIFE SERVICE HIDING INFLUENCE OF REFUGE DRILLERSubmitted by Ted Williams on Fri, 07/25/2008 - 07:51.
Federal Court Asked to Force Government to Release Records
Contact: Ceal Smith [Citizens for San Luis Valley Water Protection Coalition] (719) 256-5780;Travis Stills [Energy Minerals Law Center] (970) 375-9231; Jeff Ruch [PEER] 202-265-7337
ALAMOSA, Colo. – The federal government
Oil secrets' nasty side effectSubmitted by Ted Williams on Fri, 07/25/2008 - 07:47.
Future of Trout?Submitted by Ted Williams on Fri, 07/25/2008 - 07:46.
BigOil drools over Arctic potentialSubmitted by Ted Williams on Fri, 07/25/2008 - 07:41.
That sinking feelingSubmitted by Ted Williams on Fri, 07/25/2008 - 07:39.
EPA Issues Landmark DecisionSubmitted by Ted Williams on Fri, 07/25/2008 - 07:38.
Decision Will Improve Human Safety and End Poisonings of Birds
(Washington, D.C. – July 24, 2008) The U.S. Environmental Protection Agency (EPA) has announced a proposed decision that residues of carbofuran, a toxic pesticide that is used on a variety of crops, will no longer be allowed
Iowa turtle poaching arrestsSubmitted by Ted Williams on Fri, 07/25/2008 - 07:32.
Ontario Proects Boreal ForestSubmitted by Ted Williams on Fri, 07/25/2008 - 07:30.
Nobel Prize Winning Climate and Conservation Scientists Call Visionary Plan to Protect Vast Canadian Boreal Forest Unprecedented.
One of World's Largest Conservation Commitments: Ontario to preserve one-half of its Boreal Forest, more than 55 million acres
(Seattle, Wash.) –
Major Victory for Fish and WildlifeSubmitted by Ted Williams on Fri, 07/25/2008 - 07:26.
This great news just in from Tom France of the National Wildlife Federation. Way to go NWF!
Colleagues: As you know, Judge John Coughenour held last week that FSA had violated NEPA in approving the Critical Feed Use Program. Since then, we've gone back and forth with the government's lawyers
The First Annual TripSubmitted by Austin McPherson on Tue, 07/22/2008 - 07:29.
A couple of my friends and I decided that after college we wanted to stay in touch and still fish together occasionally. As we moved around the country, we decided the best way to maintain our friendship was to start an annual fishing trip where one person would pick the place and we would all meet


text:
- Share
Crikey takes a Commodore for a spin: is it worth saving? Beware, ABC journalists: there's another bias test coming. Has Australia caved in on the Trans-Pacific Partnership? Victorian Labor goes backwards on female representation. What they're saying about spying in Timor-Leste. And Guy Rundle weighs in on the surveillance state.
We decided to let our business editor indulge his childhood fantasies and take a V8 Commodore — the last car to be manufactured in Australia? — for a spin. Talk about kid in a candy store …
"It is stupidly fast, the gear-shift short, the steering precise, and it sits effortlessly flat through bends at speeds way over the advisory limit. The Redline's 6L V8 motor — made in Mexico by GM — was prodigious. I can't speak for the other luxury makes, but this is an awesome car — the best I've ever driven …"
But then Paddy Manning had to fuel this beast up:
"After some 402 kilometres of city and highway driving, however, my first tank takes 61.7 litres costing $102.40, and I've got a bit over 15L/100km. Fun, but clearly ridiculous in an age of inexorably rising petrol prices and worsening climate change. The clincher: at $51,490 recommended retail price, on a modest journo's wage, I could never buy one."
Can Australians afford this car? At the pump, or out of their taxes in government support?
We've long argued taxpayers shouldn't have to prop up manufacturers rejected by consumers. But Manning makes an alternate case: the subsidies are cheap in comparison, and the real waste would be in letting Holden leave …
"Are we so committed to yanking subsidies to the car industry that we are prepared to outlay more money in foregone income tax revenue and welfare benefits and subsidies like payroll tax concessions for other industries that will be needed to prop up the economy, especially in South Australia? Is it rational to spend more money destroying an industry than it would cost to save it?"
We're a broad church. With at least one rev-head.
1paddyPosted Wednesday, 11 December 2013 at 1:09 pm | Permalink
LOL and that was only your business editor.
He's an absolute sceptic compared to Possum Comitatus, who puts his money where his heart is and actually drives a Holden V8 mistress.
2ARPosted Wednesday, 11 December 2013 at 2:05 pm | Permalink
P76 redux - no Bourbon like a biz journo., forget nowt & learn less.
3klewsoPosted Wednesday, 11 December 2013 at 2:06 pm | Permalink
How much is the resultant unemployment benefits (of an Abbott government) and the domino effect of this going to cost? Not just monetarily.
4klewsoPosted Wednesday, 11 December 2013 at 5:15 pm | Permalink
2017? Damn that Carbon Tax…..?


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A meerkat enclosure has been turned into a graveyard to celebrate Halloween.
The eleven mammals at Edinburgh Zoo have been treated to spooky treats to mark the haunted holiday.
Tombstones stuffed with food and carved pumpkins filled with insects and mealworms greeted the meerkats as they ventured into their transformed enclosure on Tuesday.
The animals arrived at the visitor attraction in February and have been a popular sight ever since.
Michael Livingstone from Edinburgh Zoo said: "Halloween is a great time for us at the zoo, as we're constantly thinking of new ways to add to and enrich the lives of all our animals here, so we really enjoy getting into the Halloween swing of things.
"This is the first Halloween for our mob of meerkats, so we came up with a zombie graveyard theme for them to explore. They've never had anything like this before, so it will be exciting to see what they make of it.
"As their food will be hidden in the pumpkins and the tombstones they'll have to work to get their food, the clan will need to forage and problem solve – which are all natural skills and behaviours they would use in the wild when it came to hunting for food.
"They are an incredibly lively, feisty and curious bunch, as we see anytime we add something new into their enclosure as they all come running towards it tails in the air ready to investigate."
More About Strange Scotland
- Funding sought to fix Glenfinnan's leaning monument near Fort William
- Oldest tree in the UK is undergoing a sex change, botanists say
- 'Neglected' donkey left tied to fence was actually a garden ornament
- Excess seaweed forces partial shut down of nuclear power station
- Unusual weather phenomenon caught on camera in Western Isles
- T in the Park contractors unearth ancient roundhouse at new site
- Passenger plane swerved to avoid 'house-sized' weather balloon
People who read this story also read
- Cannabis worth £100,000 seized in police raid on city centre flat
- Thousands of pounds of jewellery and cash stolen in sneak thefts
- More than 50 companies want to offer axed Hall's staff new jobs
- Man discovered dead on remote East Lothian beach identified
- Man tried to kill friend after he saw him kiss his girlfriend


text:
Tags: fetish, submission, domination, kinky, slave
Pornstars: Winter Sky, Mark Davis
Submited: 7 years old
Views: 103779
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Section 2257
Redhead slave blindfolded and tortured in this Bdsm - Poor redhead slut takes all the pain here and screams loudly enduring this twisted fetish rituals.She craves domination and now shes all hogtied and satisfied,Check out this brutal video from SexandSubmission.Enjoy guys.


text:
ivy by godfreyescotaWatch
©2011-2016 godfreyescota
chick fanart #7 poison ivy
sexy sweet
Love it. Ivy is deadly and sexy and the pic capture that feeling!
Wow! Never saw that pose before.
Ivy with tan lines? Me gusta
galing idol!!!!!!!!
Shiiiiieeee....I would catch poison oak for that!! Fantastic job!!
Awesome work, I love that snide "Want me, can't have me" expression she's sporting.


text:
Welcome to the
Center for Instructional Development and Technology!
Our mission is to assist Southeastern faculty and staff in achieving their instructional, research, and other professional objectives by providing support for commonly used and emerging technologies. The CIDT works closely with faculty and support service providers to coordinate and promote campus-wide, technology-related services.
CIDT is located on the main campus of Southeastern on the third floor of the Russell Building, Room 317A. Our mailing address is 1405 North 4th, PMB 2755, Durant, OK 74701.
Services offered
- BlackBoard Training (Schedule an individual or group session!)
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Request a Blackboard course
Christala Smith, M.A. (email@example.com)
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Office: 580-745-3185
Cell: 580-380-1766
Justin Wallace, B.S. (email@example.com)
Instructional Technologist
Office: 580-745-3185
Kirby Shannon – Student Worker
Elementary Education Major


text:
Legendary Super Saiyan Bust, Brolly 2012 by cg-sammuWatch
©2012-2016 cg-sammu
So I grew up watching dbz when I was young back in the day..long before it was ruined by english dub..LOL anyways, I totally loved this series, just thought I make a tribute to my childhood cartoon and make the mighty Legendary Super Saiyan, Brolly in Dynamesh
Whoa that looks... solid. Well done.
Amazing and spooky at the same time
Who made this? This is so amazing, just made by a talentous person! Good job :3
Lol..I made this hahaha and thank you! XD
Lol, so epic! you have the talent on your blood, you're a true artist. This looks like Akira Toriyama's art xD I was thinking that he made this before I saw you hahahahaha congratulations! そして、あなたを知ってうれしい!╯ω╰
oh really? Wow! that a huge compliment thank you! Man, I wish some day..these big companies can offer me a toy creation deal...and I'd just do that all day LOL
I wish they call you one day too! What a amazing talent. And you made my favorite character, Broly I hope they call you, man (>^ω^<). Well, if u are from Japan or is in Japan, I think is more easy, don't you think? xD man, I really want to back to Japan one day, haha. Cool job, keep that talentous artist!
I want to own a real bust of this!
wow! beautiful!
Love this! Really appreciate the detail that went into making this!
Would love to see more.
Would love to see more.
Magnificent work!! He kind of reminds me of Dio Brando from JoJo's Bizarre adventure!
Dbz+Asura's wrath
Oh I mean Brollysan
Gokusan really likes to focus on those traps lol
Awesome piece dude. Totally bad ass!
The more blonde and spiky my hair gets the better I become at beating the shit out of people. That's logic, my friends. USE IT WISELY.
Epic! Really well done!
If only the hair could shine, it would make an awesome night light!
sooo want that in my room
amazing !
Where can I buy this!
Perfectly sculpted the details on Broly!.. Truly amazing! Kudos cg!
Just curious to know, are you working on the full version of the same piece?
Just curious to know, are you working on the full version of the same piece?
SUPREMELY sculpted! Those traps alone look like they could wipe out a galaxy or two!
simply epic i must say!
im interested to turn this nto paper craft... what do you say?
im interested to turn this nto paper craft... what do you say?
nice brolly man!! awesome
This is the best bust what i've ever see in my life!
this is absolutely amazing. can i buy one from you?
Amazing Brolly.
nice !
Very cool!
seriously... even though I've long given up on DBZ YEARS AGO... this kicks some serious ASS.
OMG. TELL ME HOW TO GET THOSE TRAPS. lol. but srsly, holy shit you got some mad skills
wow... this is so good i cant come up with a good metaphor to explain how good it is
Broly... Isn't it a shame he's evil?
if you grew up with DBZ, you had a good child hood.


text:
- Support for Industry-Leading Hardware Vendors Through Enhanced Hardware Enablement. Red Hat Enterprise Linux 5.9 showcases the strong relationships Red Hat has with leading hardware vendors by including support for some of the latest CPU, chipset and device driver enhancements.
- Continued Commitment to Security, Standards and Certifications. Red Hat Enterprise Linux has always been built with security in mind – a commitment that Red Hat Enterprise Linux 5.9 helps solidify. This update features tighter security controls, the ability to verify and check the robustness of new passwords and support for the latest government password policy requirements. It also adds support for using Federal Information Processing Standard (FIPS) mode with dmraid root devices. FIPS mode now supports RAID device discovery, RAID set activation, and the creation, removal, rebuilding and displaying of properties.
- New Developer Tools. Red Hat Enterprise Linux 5.9 includes several new developer-friendly features and tools, including the ability to develop and test with the latest version of open source Java available through OpenJDK 7. Many new SystemTap improvements have been added to Red Hat Enterprise Linux 5.9, including compile-server and client support for IPv6 networks, smaller SystemTap files, faster compiles, and compile server support for multiple concurrent connections.
- Enhanced Application Support. Red Hat Enterprise Linux 5.9 includes a new rsyslog5 package, which upgrades rsyslog to major version 5 and is faster and more reliable than existing rsyslog packages available in previous RHEL releases. Samba has also been updated to version 3.6 with several new features, including SMB2 support, a reworked print server and security default improvements for all versions.
- New Virtualization Capabilities and Flexibility in Multi-vendor Environments. Red Hat Enterprise Linux 5.9 enhances the operating system's usability in multi-vendor environments by introducing Microsoft Hyper-V drivers for improved performance. This enhances the usability of Red Hat Enterprise Linux 5 for guests in heterogenous, multi-vendor virtualized environments and provides improved flexibility and interoperability for enterprises.
- Better Subscription Management. Red Hat Enterprise Linux 5.9 uses Red Hat Subscription Management as the default, allowing customers to effectively and more easily manage their Red Hat Enterprise Linux subscriptions locally or with tools such as Subscription Asset Manager which was enhanced to easily run reports on subscription distribution and utilization and an improved user interface. For more detail on how Red Hat customers can manage their subscriptions, please visit the Subscription Management section of the Red Hat Customer Portal.
Red Hat Announces General Availability Of Red Hat Enterprise Linux 5.9
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text:
Brief comments on a garden variety of issues in the news....
Does the Constitution authorize the federal government to compel you -- or anyone -- to buy an electric toothbrush? What about a hula hoop? Rutabagas or Twinkies? A robotic vacuum cleaner? No? Then where do Congress and the Obamians get off saying the Constitution authorizes them to compel everyone to buy health insurance?
Pollsters at the Pew Research Center asked 1,500 adults to use one word to describe their current view of Congress, now with about a 25 percent approval rating -- "just the first word that comes to mind." The most frequently volunteered response: dysfunctional. Others with a strong frequency rating: corrupt, self-serving, self-centered, selfish, self-absorbed, inept, confused, incompetent, ineffective, lazy, bad, suck(s), crook(s), crooked, disappointing, gridlock, deadlock, idiots, idiotic, slow, mess, messed-up, messy, lousy, terrible, disorganized, unorganized, divided, good, not good, stupid, children, childish, childlike, do-nothing, failure, inadequate, greedy, joke, jokers, partisan, socialist, useless, worthless, bull(expletive), chaos, clowns, frustrating, horrible, inefficient, liberal, liars, money-hungry....
Newly-elected pols tend to do some good things, some bad. Among the more intelligent actions by Virginia's new governor, Bob McDonnell: he deleted from public employee health plans several million dollars in state funding for coverage of erectile dysfunction drugs.
A leading Iranian mullah has located the cause of "earthquakes" in the promiscuousness of "many women who do not dress modestly (and) lead young men astray." So should we dismiss, as well, the blandishments offered by similar Iranian lofties about their peaceful intentions for the nuclear weapons they are now (the CIA says) capable of building?
Andy Stern recently announced his retirement (after 14 years) as president of the Service Employee International Union (SEIU), government's leading union by membership. Visitor logs released by the White House in October showed Stern to have visited 22 times in the first nine months of the administration, clearly making him a favored Obamian ideological soulmate. So the President has named Stern to a federal commission weighing how to reduce the fast-rising federal deficit. He's an apt choice, having told Congress he believes "we need more...deficit spending."
Ross Mackenzie lives with his wife and Labrador retriever in the woods west of Richmond, Virginia. They have two grown sons, both Naval officers.
Be the first to read Ross Mackenzie's column. Sign up today and receive Townhall.com delivered each morning to your inbox.
Trump: If Ted Cruz doesn't stop cheating and attacking me, I have standing to sue him over his natural-born status | Allahpundit


text:
Catholic Church Threatens to Withhold Social Services if City Passes Same-Sex Marriage Law
Well, this is really shameful.
For most of the Fall, we've been reporting on the latest strains on homeless services around the city: First, it was Central Union Mission struggling to find space for a new shelter; Next, we focused on budgetary concerns crippling shelters around the city.
Well, forget all that. Now, we have a new reason to worry key social services will soon evaporate: The Catholic Archdiocese of Washington claims that it will discontinue contracts with the city if it is forced to extend employee benefits to same-sex married couples if the D.C. Council's gay marriage bill passes into law.
So the Archdiocese's priority list apparently places "helping people with desperate needs" below "not recognizing same-sex marriages."
The Washington Post has a front page article on this topic in this morning's paper.
"If the city requires this, we can't do it," Susan Gibbs, spokeswoman for the archdiocese, said Wednesday. "The city is saying in order to provide social services, you need to be secular. For us, that's really a problem."
According to the story, "city leaders"—particularly At-large Councilmember David Catania—are claiming that Catholic Charities, which serves 68,000 residents, isn't the end-all, be-all of social services in the District. (Catholic Charities also has contracts providing health care and adoption services, according to the Post.) But last month, as the Council and the Department of Human Services went back and forth on budgetary matters, Catholic Charities was one of the loudest and most pressing voices arguing for more funding to keep open full-time shelters during the winter.
The Post article quotes Ward 3 Councilmember Mary Cheh saying "she hopes the Catholic Church will reconsider its stance."
"'Are they really going to harm people because they have a philosophical disagreement with us on one issue?' Cheh asked. 'I hope, in the silver light of day, when this passes, because it will pass, they will not really act on this threat.'"
Yeah, let's hope so, for the love of god—and I guess, in this case, I mean that quite literally.
Image by TheChristianAlert.org, Flickr Creative Commons Attribution License.


text:
The votes are in!
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text:
Envy on Instagram – Everyone's life seems better on the Internet. Social media is full of posts that we decide are worthy for others to see. Is this causing us to be envious of other people's lives by comparing the snippets we see on social media to our own lives? (Relevant Magazine)
Brennan Manning Passes Away – Brennan Manning, best known for his book, The Ragamuffin Gospel, passed away on April 12 at the age of 78. After a life of sharing the gospel of grace, Manning passed after many years of declining health. (Christian Post)
Americans Love the Bible..In Theory – According to a new survey, more than half of Americans think the Bible has too little of an influence on current culture, yet only one in five Americans said they read the Bible on a regular basis. (Religion News Service)


text:
Sancho Wrestler Costume
Be wear as this is not "Sancho Panca", this is "Sancho El Luchador"! Fear him as he will do a Full Nelson on you. that is, if there is no burrito shop's around for Sancho cannot concentrate on anything else if there is a good burrito in the premises.
Available in a standard size only. Check size guide for more details regarding this size.


text:
Little Big Mission by YazmoogleWatch
©2009-2016 Yazmoogle
Mike bought him from ebay :] And later that night LBP sent him a plush for winning something. Its. Adorable.
Woo Sackboy!
this picture is awsome!! wtg! one of my favorite pics now!
Haha, love it!
I can't get over how cute this is!
hahahahahahaa that is cute!
Sackboy adventures, awesome lol
D'awww.... You should do a series of photos with this guy.
That this awesome. I want one to keep company on my study room.
they're only £6! I took him to work with me the other day and.... honestly? You can't be sad whilst looking at that little guy, you just can't.
... I think he could bring peace on earth, I really do!
... I think he could bring peace on earth, I really do!
great photo
i love sackboy
i love sackboy


text:
ADA Single Light Wall Sconce from the Revolution Collection
Simple, clean, contemporary and energy efficient, this ADA Single Light Wall Sconce from the Revolution Collection features Transitional, yet Classic Design and a Etched Clear Glass to accentuate the glass shade to offer a clean contemporary look and feel.
- Transitional, yet Classic Design
- Etched Clear Glass
- Requires (1) 25w Halogen G9 Base Bulbs (Included)
- Fixture may be mounted as an up light or down light.
- UL Listed for Damp Locations
- Height: 5.25"
- Width: 5"
- Extension: 2.75"
Additional Forecast Lighting Links
- View the Manufacturer Warranty
- Browse All Forecast Lighting Products
- Forecast Lighting Revolution Collection
This Forecast Lighting item can also be referenced by the following color/finish specific model #:
- Forecast Lighting F156468*
Deep Bronze
- Forecast Lighting F156470*
Merlot Bronze
- Forecast Lighting F156436*
Satin Nickel
*Discontinued model or finish
More Than Just a Store - Your Reasons to Shop
Discover the best online deals for the F1564 and everything else for your home from the leading online home improvement store.
We're committed to providing you with the best assortment of Forecast Lighting products with over 400 warehouses across the country.
Our knowledgeable product specialists work to bring you the best prices on every product and provide unmatched customer service. Your satisfaction is our highest priority!
Manufacturer Resources
Product Videos
Do It Yourself: Installing an Indoor Wall SconceFollow Kyleen Bromley of Build.com as she shows you the step-by-step process of replacing and installing an indoor wall sconce.
Installation Resource Center
Whether you are installing the product yourself or hiring a professional, our installation Resource Center has everything you need to get your project done. Visit the Installation Resource Center to get started.


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SIXTY SoHo
60 Thompson Street, SoHo, New York City, NY 10012, United States of America – This neighborhood is a great choice for travelers interested in restaurants, food, and shopping – Check location Show map
Excellent location – rated 9.5/10! (score from 522 reviews)
Rated by guests after their stay at SIXTY SoHo.
|Room Type
|Room Selected at Check In
|Superior Queen Room
|Superior King Room
|Deluxe King Room
|Deluxe Double Room
|King Suite
|Studio King
|Deluxe Queen Room
|Sixty Suite
|Sixty Loft
Our hotel mirrors the stylish surroundings of one of NYC's mot vibrant neighborhoods: SoHo. Stay in big city style, relax in newly-renovated guestrooms and suites, and enjoy stunning spaces at this upscale SoHo hotel.
SIXTY SoHo takes special requests – add in the next step!
6 Reasons to Choose SIXTY SoHo
Low rates
710 properties in New York City
Manage your bookings online
522 independent reviews
They speak 5 languages
Booking is safe
Facilities of SIXTY SoHo Great facilities! Review score, 8.4
- Sun Deck
Pets are not allowed.
Food & Drink
- Restaurant
- Bar
- Breakfast in the Room
Free! WiFi is available in all areas and is free of charge.
Private parking is available on site (reservation is not needed) and charges apply.
- Room Service
- 24-Hour Front Desk
- Tour Desk
- Baggage Storage
- Concierge Service
- Laundry
- Dry Cleaning
- Shoeshine
- Fax/Photocopying
- VIP Room Facilities
- Newspapers
- Safe
- Non-smoking Rooms
- Facilities for Disabled Guests
- Family Rooms
- Elevator
- Heating
- Hypoallergenic room available
- All Spaces Non-Smoking (public and private)
- Air Conditioning
Languages Spoken
- Italian
- French
- Spanish
- English
- German
From 3:00 PM
Until 12:00 PM
Cancellation and prepayment policies vary according to room type. Please enter the dates of your stay and check what conditions apply to your preferred room.
Children and Extra Beds
All children are welcome.
Up to two children under 18 years stay free of charge when using existing beds.
Extra beds are not available.
One child under 2 years stays free of charge in a crib.
The maximum number of cribs in a room is 1.
Pets are not allowed.
Cards accepted at this property
Hover over the cards for more info.
1 Appealing Option:
- 1 Hotel Central Park has a review score of 9.1
Guests are required to show a photo ID and credit card upon check-in. Please note that all Special Requests are subject to availability and additional charges may apply.


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Protests and Demonstrations
Million Mask March Demonstrators Clash With London PoliceThe anti-capitalist protesters are expected to gather in more than 600 cities worldwide.
Black Lives Matter Activists Disrupt Jeb Bush RallyThe Republican presidential candidate's town hall meeting in North Las Vegas ended amid chants of "Black lives matter."
South Carolina Gov Haley: Take Down Capitol's Confederate FlagAngry crowds marched on the Statehouse to demand the flag be taken down on Saturday.
Protests in Wisconsin After Police Officer Cleared in Shooting of TeenA prosecutor ruled that a Madison police officer's fatal shooting of unarmed biracial teenager Tony Robinson was justified.
Photos: Baltimore Erupts: 12 Arrested in Protests Over Death of Freddie GrayGray died April 19 after suffering an unexplained spinal injury while in police custody.
Revisiting the Violent, Sometimes Wacky World of America's 1970s Radical UndergroundBryan Burrough reconsiders the left's 1970s revolutionary underground in his new book, 'Days of Rage.'
Ferguson: When Cops Become Cash CowsWhen police departments become profit centers, justice goes out the window.
On MLK Day, Police Reform Advocates Take Message to UNActivists in New York City organized a '#Dream4Justice' march Monday to advocate for police reform.
Protests, Celebrations Planned For Martin Luther King Jr. DayA list of events across the country that will commemorate the civil rights leader Monday.
Martin Luther King Jr.'s Dream LivesToday's protests and the release of 'Selma' show the continued relevance of civil rights struggles.
Thousands Attend Anti-Islam Protests in GermanyChancellor Angela Merkel and other senior German politicians have called for people to stay away from rallies organised by Patriotic Europeans Against the Islamisation of the West.
50 Prominent Germans Condemn Anti-Muslim RalliesAnti-Muslim rallies have become a weekly event in Dresden, with some 18,000 people at a rally Monday
More People Detained For Threats Against NYPDSix people have been detained so far for threats against NYPD officers since the fatal shooting of two officers in New York on Saturday.
Protests in Missouri After Gas Station ShootingOfficials in Missouri were at pains to distinguish the death of the suspect, whom they noted was holding a gun, from cases of unarmed black men who had been killed by police officers.
De Blasio to Protesters: 'Politics and Protest Can Be for Another Day'New York City Mayor Bill De Blasio asked protesters to suspend their protests in light of the recent killings of two NYPD officers.
Man Protests on Ledge of St. Peter's in VaticanMarcello di Finizio is protesting against laws he says caused him to lose him business
Can Big Data Help Build Trust in the Police?Obama's Task Force should stress transparency in hiring to increase diversity.
France to 'Ban Uber' After Taxi Driver ProtestUber's general manager dismissed the ban as a matter of opinion, saying it would continue to operate
How to Avoid Another Ferguson or GarnerCameras on uniforms and guns, more diversity for starters.
Merkel Condemns Racism as Dresden Anti-Islam Marches GrowThis week Merkel's conservatives debated banning the burka, the full body covering worn by some Muslim women
Bay Area Protests Dwindle, Looting ReportedWitnesses report an undercover police officer marched with the demonstrators and pointed his pistol at protesters after he and his partner were attacked.
Obama on Brown, Garner and Race in America"I want my grandsons to be treated like anyone else's grandsons," Obama said during a recent interview on BET.
After Ferguson: Life Through Black EyesBlack stereotypes of whites are just as scary as white stereotypes of blacks.
UK Police Arrest 76 at London Eric Garner 'Die-In' ProtestLondon's Metropolitan Police said a small group of protesters had fought with security
Anonymous Targets Oakland City and Police SitesActivist hackers from the Anonymous collective claimed responsibility for taking the Oakland police department and city websites were offline on Wednesday.
The Black Male Experience from the C-Suite to the StreetEven top business executives of major companies suffer racial insults -- if they're black.
Eric Schneiderman Seeks To Investigate Police Killings of Unarmed CiviliansNew York State's top prosecutor on Monday sought the power to probe all police killings of unarmed civilians in his state, following sometimes violent U.S. protests.
Rapper Turned Cop Tells Story From Both SidesOakland rapper, actor and playwright Jinho "The Piper" Ferreira didn't just complain about the cops: He became one.
Protesters Block Verrazano-Narrows Bridge in NYC over Deaths of Eric Garner, Michael BrownProtesters blocked the main thoroughfare between the boroughs Staten Island and Brooklyn in New York City.
California Protesters Clash With Police Again Over NYC 'Chokehold' DeathWest Coast cities had braced for trouble after clashes in Berkeley, Seattle and Washington on Saturday


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"The NJC has meant a great deal to me....There are relatively few opportunities for young, aspiring journalists like myself who value free-market principles and believe in American Exceptionalism."
- Jarrett Stepman, Spring 2011Human Events intern
There is no better place to be for hard-hitting news than in Washington, D.C., and there is no better way to get your foot in the door than with an internship with the National Journalism Center. The 12-week program combines weekly seminars, assigned reporting drills, and on-the-job reporting experience through internships with newspapers, magazines, broadcast outlets, and other media.
Weekly seminars include a blend of writing assignments, journalism exercises and quizzes, lectures with prominent public policy experts and journalists, and current events discussions. Interns typically work three to four days per week at a news outlet and spend one day per week at the National Journalism Center participating in journalism skills workshops.
NJC interns have recently been placed with media outlets around the Washington, D.C. area, including:
If Washington is where you want to be this upcoming spring, summer, or fall, fill out our online application. If you have additional questions about NJC, please e-mail Program Director Summer Ratcliff
CLICK HERE to apply today!
NOTE: Many media outlets in Washington require taking the internship for college credit. These placements also have earlier application deadlines. If you are interested in receiving college credit for your internship, please apply four weeks in advance of the listed deadline. Applications are also processed on a rolling basis, so it may be to your advantage to submit your completed application early. The Center will work with interested students to arrange for academic credit.


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Traci Townsend
Not Yet Rated| 1 hr. 28 min.
Plot Summary
A successful and attractive journalist interviews three previous boyfriends to see why they never proposed.
Cast: Jazsmin Lewis , Richard T. Jones , Victor Williams , Mari Morrow , Aaron D. Spears , Marlon Young , Jay Acovone ,
Director: Craig Ross Jr.
Genres: Comedy


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Every day, come rain or shine or internet tubes breaking, Film School Rejects showcases a trailer from the past.
The management of this theater is proud to suggest this movie for every member of every family everywhere.
It's fascinating to think of how dark and frightening this movie is despite all the musical interludes, the trademark Disney animation, and all the fairies prancing around making mops do their cleaning work for them.
Yes, it's the story of Princess Aurora, Prince Phillip, and a kiss that can break a coma.
Check out the trailer for yourself:
Let the film marketers of the past sell you on their movie by checking out more VTOTDs
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text:
Hnossa: EED 11/04/13 - Welcome Fern Elizabeth 10/30/13
JW82: EDD 11/9/13 - Welcome Tristan 11/9/13!
Mominheels: EDD 11/12 - Welcome Baby Hunter Kane 10/13/13
Mirracle34: EED 11/16/13 - Welcome Baby Boy 11/16/13
BarbRT: EDD 11/18/13 - Girl
MommyofJD10: EDD 11/18/13 Welcome Quinn Olivia 11/05/13
HopeReflected: EDD 12/4/13 Girl
Rdebellis: EDD 12/9/13 Girl
Meggo1: EDD 12/10/13 Welcome Ethan 11/27/13
Madcurlz: EDD 12/13/13 Girl
Baby220131: EDD 12/16/13
Angel2mommy78: EDD 12/16/13 Boy
Hopeafterheartbreak: EDD 12/20/13 Girl
Varela11: EDD 12/20/13 Girl
JennaLKay: EDD 12/22/13
Northy13: EDD 12/23/13 Welcome Charlie and Olivia 11/22/13!
Kmjd2: EDD 12/25/13 Boy
LillyBelle813: EDD 12/31/13 Girl
Raneeisme: EDD 1/2/14 Welcome Bronson 11/22/13!
Lizzythorn: EDD 1/4/14 Surprise
A-tomka: EDD 1/5/14 Girl
M&MinMay: EDD 1/7/14 Girl
RainbowsOneDay: EDD 1/8/14 Girl
Lola&Gertie: EDD 1/11/14 Surprise
Law80: EDD 1/12/2014 Boy
mbmays: EDD 1/12/2014 Surprise
Seplowla: EDD 1/16/14 Boy
Newbieadie: EDD 1/22/14 Girl
AlwaysLoveG: EDD 1/23/14 Girl
Cleareyesfullheart:EDD 2/10/14 Boy
hotpeppers17: EDD 2/10/14 Boy
ktozzi1130: EDD 2/14/14
Patiekmm0305: EDD 2/18/14 Welcome Josh 11/26/13
stressfreepregnancy: EDD 2/20/14 Girl
Meghnat1: EDD 2/21/14 Boy
RobinNYC5: Edd 2/21/14 Boy
Megsme: EDD 2/23/14 Girl
Peanutll: EDD 2/25/14
50208: EDD 3/5/14 Boy
MLC4684: EDD 3/7/14 Girl
Velvetcaress: EDD 3/12/14 Boy
Devastated15: EDD 3/14/14 Girl
Sunnnnaaayy: EDD 3/16/14Girl
emilytufte: EDD 3/16/14 Boy
mtmoose: EDD 3/20/14 Girl
haake426: EDD 3/21/14 Two Surprises
Porcelina1: EDD 3/24/14 Girl
MrsBlack423: EDD 3/31/14 Boy
maryis: EDD 4/5/14 Surprise
tfancey: EDD 4/5/14 Girl
TinyE2: EDD 4/5/14 Boy
rhd07: EDD 4/8/14 Boy
TweezerReprise: EDD 4/11/13 Girl
NICU Rainbows- Thinking of you and cheering you on!:
Northy: Charlie and Olivia
Raneeisme: Bronson
Patiekmm0305: Josh
Hi everybody! Figured I'd take a turn hosting this week :) How is everybody doing?
QOTW: You know I've got to ask how everyone's nursery is coming along- I swear it's all I can think about anymore!! I'm starting to get furniture assembled and decorations up, but it still looks a mess! I always wanted to do a picture wall in the nursery of family baby pictures, but when I started getting things framed I felt bad that my angel wasn't included (I suspect this will be a recurring theme throughout my life :( ) so I just did a simple framed piece that says "LOVE" with my angel's footprints as the V.


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Regional Workshops: Detroit
Michigan Historic Preservation Network
Conserve Energy – Preserve Resources – Create Sustainable Jobs
FREE Plaster Repair–Preservation Trades Training
March 23–March 27, 2015
Grand Army of the Republic (G.A.R.) Building
1942 Grand River Avenue, Detroit, MI 48226
In an intensive five-day workshop, students will learn the fundamentals of traditional plaster repair. This workshop seeks to expand the skills of workers with some existing trades knowledge rather than introduce individuals with no previous trade experience. Trainees will work with traditional building materials under the guidance of trained preservation craftspeople. The training will be conducted in partnership with Integrity Building Group.
Prospective trainees need to:
1. have an interest in pursuing historic preservation work as a vocation or avocation.
2. possess appropriate fine motor skill abilities and physical strength to successfully complete the work of the class.
3. agree to follow all safety instructions, and use all available safety and personal protection clothing and equipment as instructed.
4. sign a release of liability form and a model release—photos and video from the training may be used to promote preservation trades training both within Michigan and elsewhere.
5. commit to the full five-day workshop and be on-site by 9:00 am each day.
6. be at least 18 years old.
7. bring a lunch each day.
The workshop will provide:
1. all necessary tools – trainees may bring their own tools but they should be clearly marked.
2. EPA RRP Lead Renovator Initial Course, qualifying the trainee as a Certified Renovator (if trainee is not currently certified).
3. safety gear as needed.
4. refreshments each day.
Application must be received by: Tuesday, March 10, 2015 at 5:00 p.m.
Applications may be e-mailed, faxed, or mailed.
By e-mail: with an attachment to email@example.com
By fax: Attn: "Detroit Plaster Workshop" to 517.371.9090
By mail: Allow time for mailing to: MHPN,
Attn: "Detroit Plaster Workshop," 313 E Grand River, Lansing, MI 48906
Space is limited. Trainees will be chosen by Friday, March 13th, 2015.
A waiting list will be kept. Questions? Please address inquiries to "Detroit Plaster Workshop" at MHPN: email@example.com or 517.371.8080.
The workshop is free thanks to our sponsors:
Michigan State Historic Preservation Office (SHPO), Michigan State Housing Development Authority (MSHDA), the Michigan Historic Preservation Network, and Integrity Building Group


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By Guest Contributor Tami Winfrey Harris, cross-posted from What Tami Said
Hmmm … I'm just digesting Joan Walsh's analysis of the Cornel West v. President Obama controversy. Now, I think West's attack on Obama was petty, personal and, perhaps worst of all, an example of destructive policing of blackness from within. So, I was with Walsh until she went here:
But there's a way in which this whole controversy looks like progressives devouring their own tail. From the left, West attacks Obama for not being black enough; I've written about being attacked as a clueless, entitled white progressive for criticizing Obama; in a pro-West backlash, black Obama supporters are being dismissed as "elitist" fronts for white liberals and that half-white guy in the White House. It's crazy. Read more…
Whoa … whoa … whoa there, Joan! In her article, Walsh goes from pointing out the silliness of "not black enough" charges to using West's foolishness to imply that analysis of political opinion through the lens of race and other identities is without merit–particularly when leveled at, well, Joan Walsh.
Read the Post No, Joan Walsh, racial criticism does not equal 'identity politics'


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Random PNGs - 2001 by MissesglassWatch
©2012-2016 Missesglass
Random PNGs
Comments and faves are very much appreciated
Hope you enjoy this set!
Comments and faves are very much appreciated
Hope you enjoy this set!
Lovely! Thanks!
-ขอบคุณมากๆจ้า ^^
thx dearoo so cute
SOOOOOOO cute
Graciias *me gustaron muucho*
I love it
muy buenos
very cute!
take it...
take it...
nice !!!
so cute ^^
i love it <33
i love it <33
OMG So Cute! *--*
I Love!
I Love!
so cute .. lovely ^^
how you cut your pngs so good? i make pngs too but I can't completely remove the background. remains an ugly border ... yours are perfect instead *-*
First of all, thank you! I don't know which program you use, but I use Photshop. I cut my images by using the Polygonal Lasso Tool and it works perfectly.
It is very cute
Thank you for sharing
Thank you for sharing


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Accepts Credit Cards
Price Point
$ $ - Cheap Eats (Under $10)
$$ - Moderate ($11-$25)
$$$ - Expensive ($25-$50)
$$$$ - Very Pricey (Over $50)
Good for Kids
Outdoor Seats
E & L Barbeque ($)
American, Barbecue, Chicken, Steak, Burgers
Boston Fish Supreme ($)
American, Seafood, Chicken
Dixon's Diner ($)
Diner, American, Burgers, Sandwiches, Soups
Bonsai Japanese Steakhouse ($$)
Japanese, Sushi, Steakhouse
Is this your restaurant? Click to add your description here.
Accepts Credit Cards
Price Point
$ $ - Cheap Eats (Under $10)
$$ - Moderate ($11-$25)
$$$ - Expensive ($25-$50)
$$$$ - Very Pricey (Over $50)
Good for Kids
Outdoor Seats
06/12/2009 - Louis Bernhardt
June 10, 2009 *

 Located Downtown near the Mississippi State Coliseums: 929 High Street * Jackson, Ms. 39202
 *For Take Out Orders call: 601-944-0024

 Open Daily Monday-Saturday from 7:00 A.M. until 2:00 P.M.

 * For the best in breakfast and lunch time meals fixed to perfection; come to the Famous Farmers Market Grill of Jackson, Mississippi located downtown at 929 High Street. Home of Southern Style Home Cooking, just like Grandmother fixed on the special occasions. When you are hungry and desire a great breakfast or lunch come on down and enjoy a special meal! If you have a company meeting going om and can not leave, then just give us a call. We will have you food ready in just a short time for pickup. Complete catering for all events. Serving 25-500 people with quality food of your choice.

 * Call 601-944-0024 for complete catering details */h3>

 Daily Lunch Menu

 * Monday: Fried Chicken, Meatloaf, or Roast Beef with 3 veg tables.
 *Tuesday: Red Beans & Rice, Country Sausage, or Fried Chicken with 3 vegetables.
 *Wednesday: Spaghetti with Meat Sauce, Chicken & Dumplings, or Fried Chicken with 3 vegetables.
 * Thursday: Pork Chops, Hamburger Steak with onions, or Fried Chicken with 3 vegetables.

 * Friday: Catfish, Baked Fish, or Fried Chicken with 3 vegetables. Saturday: Fried Chicken, Hamburger Steak, or Pork Chops with 3 vegetables.

 * Daily Lunches only $7.25 Includes Tea, Cola, or Bottle of water plus bread. *

 Daily Vega table include: Rice & Gravy, Potatoes (fried, baked, or mashed with gravy), Sweet Corn, small green salad, green peas, green beans, baked beans, okra, asparagus, and sweet potatoes. Daily Breakfast Served Starting at 7:00 AM -11:00 AM. Bacon & Eggs or Ham & Eggs with grits and toast only $5.95! Free coffee or tea! Take Out Specials: 601-944-0024 Jackson, Mississippi, Farmers Market Grill, downtown,daily lunch specials,country breakfast, hot gravy,sausage gravy, ham and eggs,bacon and potatoes, sandwiches, BLT sandwich, Louisiana Po-Boy Sandwiches to go or eat here. Read more: Community-Announcements-Breakfast-Lunch-Takeout-Services-Downtown-Jackson-Mississippi * Menu * Fast Meal & Breakfast Delivery *

 EVENT CATERING * CATERS * FOOD to GO : Grill & Deli * Location: Service area includes : MISSISSIPPI: Jackson : Pearl : Clinton : Raymond : Vicksburg; Hinds Warren Rankin County. Our location is on the Mississippi State Fairgrounds & Coliseum * We are inside the Farmer's MARKET Building! 921 High Street : Jackson, Mississippi 39202. TELEPHONE: (601) 944-0024. (Orders to Go * Delivery)

 DELIVERY AREA* Limited to Downtown Area.
 MINIMUM DELIVERY ORDER= $20.00 * $4.00 DELIVERY FEE
 SERVICES OFFERED: Restaurant - Grill - Deli -Event - Wedding - Corporate Events - Church & Social Events - Outdoor Bar-B-Q - Catering - Catered - Caterers - Deli & Sandwich Trays - Vegetable Platters & Trays - Shrimp Platters & Trays - Deli, Sub, & Poor Boy Sandwich Trays - Events catered from 25 - 1000.
 EXPERIENCE: Official Caterer for the Mississippi Coliseum and having the experience of serving groups over 1100 people at one meal. These groups include the World Famous Bands and Entertainers performing at the Coliseum. Catered events have also included Rodeo & Dixie National Events.
 FOOD OFFERED: Our Menu includes full breakfast & dinner meal preparation. We cater ANYTHING on our menu! We cater anywhere and anytime within our service area. Menu Includes: Southern Fried, Bar B Q, or baked chicken. Chicken fried steaks with Southern White Gravy, Southern Fried Catfish, Mamma's Recipe Meatloaf, Grandma's Recipe Southern Style Chicken & Dumplings, Baked White Fish, and many others.
 SANDWICHES: Deli, Sub, & Louisiana Style Po-boy Sandwiches. Meatball Po-boy with our tasty Italian Red Sauce. Made to order, Our famous Catfish and Shrimp poboys are most excellent. The ORIGINAL Beef & Ham Poorboy Sandwich comes with savory brown gravy for covering or dunking the poorboy while eating a New Orleans specialty! Our sandwich trays and platters are very affordable yet outstanding in appearance, quality ingredients, and sized to your personal requirements. FOR DETAILS or to place an order call:

 (602) 944-0024

05/16/2009 - Louis Bernhardt
At the Farmer's Market Grill in Jackson, Ms. one finds Southern Cooking at it's best! Most excellent fried chicken, chicken & dumplins, home-made hamburger steaks, Louisiana Style Po-Boy Sandwiches with gravy, & delicious meatloaf served with vegatables and bread. I enjoy this type of cooking and finding a great place to eat like this is special to me. The owner Gary E. "PETE" Clark is a true Southern Gentleman with a great staff of friendly folks.
Horrible experience, every time I go to Peace Street in Canton, I have to wait on my order for at least 15 minutes, because the grill cooks are outsid... read more2. The Blue Rooster (Flora, MS)
Today was my first time in you great little resturant - all I can say is DARN GOOD - was there with my friends Charlie & Cathy - hope to dine with... read more3. JC's General Store (Flora, MS)
I went in too late for lunch, but was told "sure I'll fix you a burger". Everyone in the store from the cashier to the cook were super nice,... read more4. Caporales Mexican Grill (Byram, MS)
The food was nasty was nasty ass hell, and let's not talk about the drinks. They need to take their ass to bartender school. How you gonn... read more5. El Sombrero Mexican Restaurant (Clinton, MS)
I've been going to El Sombrero since approximately 2002...Never had a bad experience..always had friendly service. The food is reasonably priced as w... read more
Jackson Restaurant Guide: See Menus, Ratings and Reviews for Restaurants in Jackson and Mississippi


text:
We are currently designing a website that will be accessed from kiosks in a museum. Users will need to be able to browse through a number of different web pages that will currently contain static HTML content as well as go through various interactive presentations that will be based on flash and run in flash player. Users may need to input some information into a simple registration form from the kiosk as well.
One reason that we are currently designing this to run in a browser is because the same site and interactive presentations that are being accessed from the kiosks will also be available on the web.
Does anyone have recommendations on whether this design will even work or whether we should simply build two versions of the content, one for web and one for kiosk? What experiences has anyone had with building a website that is available on touchscreen and via the web?
This is a first time effort both for myself and for my company so any warnings, suggestions, recommendations at all would be greatly appreciated.
Note: We are currently working with Flash to build our interactive content and a LAMPP stack for the server-side everything so SilverLight is not really an option.


text:
Definition of mark time in English:
1(Of troops) march on the spot without moving forward.
Example sentences
- I had them mark time and started them off marching down the trail that led to the football field.
- He blew his whistle, signaling for the band to mark time.
- Still, some steps are better than just marking time in place, right?
1.1Pass one's time in routine activities until a more interesting opportunity presents itself: we're all just marking time, waiting for Wednesday
More example sentences
- But he's only marking time until he can return to New Orleans.
- In the short term the markets are still nervous and will mark time until the outlook for the US becomes more certain.
- The secondary has to find out and the kids with a high-level D have to mark time until those without catch up.
Definition of mark time in:
- US English dictionary
What do you find interesting about this word or phrase?
Comments that don't adhere to our Community Guidelines may be moderated or removed.
Subscribe to remove adverts and access premium resources
= trending


text:
Marine Hotel2 Self rated
2-6 Adelphi Road Paignton, Devon, TQ4 6AP
Price FromView Rooms
- Very Good
- 3 guest reviews
"all staff very pleasant and helpful. Rooms could do with some TLC......."
"Staff very helpful, hotel clean, spacious, although decor a little outdated, good breakfast would stay again...."
"Such a lovely place to stay, it was beautiful rooms with beautiful views, the staff where very friendly and attentive and the food was nice..."
About the hotel
A warm, friendly, family run hotel with home cooked meals situated on a level position, directly opposite the seafront and a short walk to the town centre, you couldn't ask for a better located hotel on the Riviera. With some level access rooms and a lift to all four floors the hotel is very accessible, some rooms require steps after the lift. The hotel is airy, modern and light yet highly comfortable.
After just one visit, you'll want to adopt the hotel as your second home by the sea.
Read MoreLess
Room Availability
Showing Tuesday 9 February 2016 to Wednesday 10 February 2016Change Search
Facilities Show/hide
- Main
- Hotel Non-Smoking Throughout
- Family friendly
- Cots available , Pets Allowed
- Parking
- Car parking available onsite
- In room entertainment
- TV , Radio
- In room facilities
- Tea/Coffee making facilities in each room , Hairdryer in each room , Telephone In Each Room
- Hotel services
- Wake Up Call Available
- Other
- Ground floor bedrooms , Lift to all floors
- Dining
- Bar
Useful Info Show/hide
- From 14:00 hours
- Until 10:00 hours
- The price shown is based on the occupancy and dates you have searched for. Prices and availability are subject to change based on the arrival date, number of nights and occupancy searched for. All prices are inclusive of all local taxes.
- Pets are allowed
Customer Reviews
Our reviews are from genuine LateRooms.com customers. We verify that the customer has stayed before leaving a review.
Very Good
100% would recommend it to a friend
Customer service
Food quality
Room quality
all staff very pleasant and helpful.
Rooms could do with some TLC....
Read More Less
Staff very helpful, hotel clean, spacious, although decor a little outdated, good breakfast would stay again.


text:
Expedia.ca offers a selection of 118 hotels in Perigord. You are sure to find cheap deals and discount rates among them with our Best Price Guarantee. No matter what type of hotel strikes your fancy, we will let you search for your preferred one to accommodate your travel needs.
Expedia.ca will not only save you time, but also money when booking your stay at a hotel in Perigord. Making reservations so you can stay within your budget is easy with us.
Expedia.ca even provides you with a map so you may find hotels in the Perigord neighborhood you prefer.
Go ahead. Enter your travel dates to view the best offers on hotel rooms in Perigord, France now!


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Milk Splash by TamerlanaWatch
©2010-2016 Tamerlana
I love milk. And this is the best milk splash I saw the last time
The milk looks awesome! ;D
awww I love that photo !
I saw all the pictures you took of this kind, I think clearly this is the prettiest, but the coffee gave it a brighter effect
I love the angle and the fact that if you can see the droplets to fly
Good work
I love the angle and the fact that if you can see the droplets to fly
Good work
Amazing colors
Very well captured Image
This photo is amazing! *__*
mm creamy
really great picture! love it!
so cool.
ha, great!
Got milk?
This is lovely. I love it.
oy tochno) i ne podumal by chto D5000
Na chto snyato? otlichno poluchilos'!
woah you take really good pictures with this cup
great work!
Did you just drop something down it?
really nice splashes
oh this is really a great capture (:
amazing art!
Perfect! I luv that *-*
The colors are adorable~
The colors are adorable~
cool ^^
It looks like an epic flower.
классная фотография)


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What does AWGN mean in Networking?
This page is about the meanings of the acronym/abbreviation/shorthand AWGN in the Computing field in general and in the Networking terminology in particular.
Additive White Gaussian Noise
Find a translation for AWGN in other languages:
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Discuss this AWGN abbreviation with the community:
Use the citation below to add this abbreviation to your bibliography:
"AWGN." Abbreviations.com. STANDS4 LLC, 2016. Web. 8 Feb. 2016. <http://www.abbreviations.com/term/335884>.


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Elf Face Painting by BruceCollinsArtWatch
©2011-2016 BruceCollinsArt
Water Based Face Painting
Awww, nice! ^^
hehe thats freaking awesome i love the vibrant colours i use snazaroo but it dosent have the same brightness, what brand do you use? x
Thank you ...I mix Kryolan cakes with Mehron liguid...the day glow colors work well
hhhhmmmm kryolan i think ive got the bronze cake paint by them which was good but the silver wasnt that great which put me off but after seeing thi ill give it a second go, cheers x
Haha that's really cool
that is just amazing - thought you had an actual hat on
I love this!! It's so creative- and I'm curious to see what the perspective from the other side was. ^_~
i ma so very amazed at how much I thought you were wearing a hat and glasses when I first glanced at this :')
lol yes! i'm glad this happened, very well done


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Introduction to Microbiology With Infotrac A Case-Study ApproachAuthor:
Ingraham, John L., Ingraham, Catherine A.
Pub Date: 2003
Publisher: Thomson Learning
Summary: John Ingraham, a professor of microbiology, and Catherine Ingraham, his daughter and a practicing physician, use their experience within a case study approach complemented by a CD-ROM and Infotrac.
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Pub Date: 2003
Publisher: Thomson Learning
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Publisher: Thomson Learning
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Stories tagged: Niles Walmart
Latest stories tagged: Niles Walmart
Police: Walmart arsonist visited store previous day
Police say the person responsible for starting a fire at the Niles Walmart Sunday morning was mostly likely at the store the previous morning attempting ... Read more
Fire in Niles Walmart considered arson
A fire that broke out inside the Niles Walmart early Sunday morning is being called arson, according to authorities with the Berrien County Sheriff's Department. ... Read more
Walmart hosts Facebook campaign against hunger
Walmart is giving away $2 million and is asking consumers to visit its Facebook page between now and April 30 to vote for one of ... Read more
Local cops play Santa
Buchanan police officers conducted a "raid" on the Niles Walmart Saturday. Read more


text:
"More Americans work for the government than work in construction, farming, fishing, forestry, manufacturing, mining and utilities combined," Stephen Moore reported in the Wall Street Journal recently. "We have moved decisively from a nation of makers to a nation of takers.
Nearly half of the $2.2 trillion cost of state and local governments is the $1 trillion-a-year tab for pay and benefits of state and local employees."
Yet even with all those government employees on the job, important things such as bridges and highways (generally a government responsibility) are falling into disrepair. "The original architects of such systems are now mostly dead, and we, their replacements, often lack their education and respect for civilization's protocols," historian Victor Davis Hanson wrote recently about California's formerly-great freeway system. "The result is that millions of Americans are simply enjoying a system built for them by others which they are not quite able to use, repair, expand -- or understand."
Hanson could just as well have been talking about our national power grid. It manages to provide enough juice to air condition every home and office building, even though those who designed it could never have imagined it would need to deliver so much electricity.
Meanwhile, as Steven Hayward writes, some are even working to remove a source of clean electricity. "Today's environmental Progressives see dams as high on the list of humanity's crimes against nature, and want to remove as many of them as they can."
From the railroad barons through Bill Gates, most great American fortunes were based on building things people needed and used. The owner profited handsomely, but we all benefited, and continue to benefit, from the owner's work.
Contrast that with the Bernie Madoff pyramid schemes and heavily subsidized industries that aren't held to the same standards for providing value-added products and services at a price people (and societies) can afford. If we want to save American jobs we need to reward those who create them. We get what we pay for.
Rich Tucker is a communications professional and a columnist for Townhall.com.
Rush Limbaugh defends Rubio's debate answer: He's absolutely right that Obama knows what he's doing | Allahpundit
Time for Team Hillary to break out the gender card for young liberal women who prefer Sanders | Allahpundit


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Welcome to Audiko, where you can aquire epic ringtones for your cell phone. Today we have a ringtone of "Numb / Encore" by Linkin Park as well as 8885 other ringtones by Linkin Park. "Numb / Encore" is the best track from the "Collision Course" album that was recorded by Linkin Park at their recording studio together with other 10 tracks. You can listen to all 4 Linkin Park – "Numb / Encore" ringtone variations available here and download it for free right to your phone. You can also select other 1 albums produced by Linkin Park for all time. "Numb / Encore" is referred to Rock, and we have a huge amount of Rock tracks absolutely for free. And finally, we have collected lyrics for this track and you can view it here as well. So feel free to browse, listen, download ringtones at Audiko.net. Make your phone sound cool!
Linkin Park - Numb / Encore
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Are you Torsten Unge?Claim your profile
Publications (85)407.47 Total impact
Article: Insights into the inter-ring plasticity of caseinolytic from the X-ray structure of Mycobacterium tuberculosis ClpP1[Show abstract] [Hide abstract]
ABSTRACT: Mycobacterium tuberculosis caseinolytic protease ClpP1 (Mt ClpP1) is a self-compartmentalized protease consisting of two heptameric rings stacked on top of each other, thus enclosing a catalytic chamber. Within the chamber, which can be reached through two axial pores, each of the 14 identical monomers possesses a serine protease active site. The unfolding and translocation of substrates into the chamber are mediated by associated hexameric ATPases covering the axial pores. Three crystal structures of Mt ClpP1, determined by molecular replacement, are presented in this study. Two of the models were refined to a resolution of 2.6 A and the third to 3.0 A. It was found that disorder in the handle domain affects the formation and configuration of the tetradecamer and results in condensed structures with larger equatorial pores when compared with ClpPs from other species. Additionally, this disorder accompanies conformational changes of the residues in the catalytic triad. The models also reveal structural differences within the N-terminal hairpin-loop domain, which possibly reflect the significant differences in amino-acid sequence between Mt ClpP1 and other ClpP homologues in this region.
Article: Erratum: Application of the use of high-throughput technologies to the determination of protein structures of bacterial and viral pathogens (Acta Crystallographica Section D: Biological Crystallography (2006) D62 (1196-1207))
Article: Application of the use of high-throughput technologies to the determination of protein structures of bacterial and viral pathogens. Corrigendum[Show abstract] [Hide abstract]
ABSTRACT: The Structural Proteomics In Europe (SPINE) programme is aimed at the development and implementation of high-throughput technologies for the efficient structure determination of proteins of biomedical importance, such as those of bacterial and viral pathogens linked to human health. Despite the challenging nature of some of these targets, 175 novel pathogen protein structures (approximately 220 including complexes) have been determined to date. Here the impact of several technologies on the structural determination of proteins from human pathogens is illustrated with selected examples, including the parallel expression of multiple constructs, the use of standardized refolding protocols and optimized crystallization screens.
Article: The 1.9 Å resolution structure of Mycobacterium tuberculosis 1-deoxy-D-xylulose 5-phosphate reductoisomerase, a potential drug target[Show abstract] [Hide abstract]
ABSTRACT: 1-deoxy-D-xylulose 5-phosphate reductoisomerase catalyzes the NADPH-dependent rearrangement and reduction of 1-deoxy-D-xylulose 5-phosphate to form 2-C-methyl-D-erythritol 4-phosphate, as the second step of the deoxyxylulose 5-phosphate/methylerythritol 4-phosphate pathway found in many bacteria and plants. The end product, isopentenyl diphosphate, is the precursor of various isoprenoids vital to all living organisms. The pathway is not found in humans; the mevalonate pathway is instead used for the formation of isopentenyl diphosphate. This difference, combined with its essentiality, makes the reductoisomerase an excellent drug target in a number of pathogenic organisms. The structure of 1-deoxy-D-xylulose 5-phosphate reductoisomerase from Mycobacterium tuberculosis (Rv2870c) was solved by molecular replacement and refined to a resolution of 1.9 A. The enzyme exhibited an estimated kcat of 5.3 s-1 and Km and kcat/Km values of 7.2 microM and 7.4x10(5) M-1 s-1 for NADPH and 340 microM and 1.6x10(4) M-1 s-1 for 1-deoxy-D-xylulose 5-phosphate. In the structure, a sulfate is bound at the expected site of the phosphate moiety of the sugar substrate. The M. tuberculosis enzyme displays a similar fold to the previously published structures from Escherichia coli and Zymomonas mobilis. Comparisons offer suggestions for the design of specific drugs. Furthermore, the new structure represents an intermediate conformation between the open apo form and the closed holo form observed previously, giving insights into the conformational changes associated with catalysis.
Article: Interaction Kinetic Characterization of HIV-1 Reverse Transcriptase Non-nucleoside Inhibitor Resistance[Show abstract] [Hide abstract]
ABSTRACT: To decipher the mechanism for non-nucleoside inhibitor resistance of HIV-1 reverse transcriptase, the kinetics of the interaction between wild type and drug-resistant variants of the enzyme and structurally diverse inhibitors were determined. Substitution of amino acid residues in the inhibitor binding site resulted in altered rate constants for the pre-equilibrium between two unliganded forms of the enzyme, and for the association and dissociation of the inhibitor-enzyme interaction. The Y181C, V108I, and P225H substitutions affected primarily the association and dissociation rate constants, while the K103N and the L100I substitutions also influenced the equilibrium between the two forms of the free enzyme. The K103N and the L100I substitutions were found to facilitate both the entry of the inhibitor into the binding pocket as well as its exit, in contrast to what has been reported elsewhere. Interaction kinetic-based resistance profiles showed that phenethylthiazolylthiourea compounds were relatively insensitive to the studied substitutions.
Article: Biosensor-Based Kinetic Characterization of the Interaction between HIV-1 Reverse Transcriptase and Non-nucleoside Inhibitors[Show abstract] [Hide abstract]
ABSTRACT: Details of the interaction between HIV-1 reverse transcriptase and non-nucleoside inhibitors (NNRTIs) have been elucidated using a biosensor-based approach. This initial study was performed with HIV-1 reverse transcriptase mutant K103N, the phenethylthioazolylthiourea compound (PETT) MIV-150, and the three NNRTIs licensed for clinical use: nevirapine, delavirdine, and efavirenz. Mathematical evaluation of the experimental data with several interaction models revealed that the four inhibitors interacted with HIV-1 RT with varying degrees of complexity. The simplest adequate model accounted for two different conformations of the free enzyme, of which only one can bind the inhibitor, consistent with a previously hypothesized population-shift model including a preformation of the NNRTI binding site. In addition, a heterogeneous binding was observed for delavirdine, efavirenz, and MIV-150, indicating that two noncompetitive and kinetically distinct enzyme-inhibitor complexes could be formed. Furthermore, for these compounds, there were indications for ligand-induced conformational changes.
Article: Microwave-Accelerated Synthesis of P1'-Extended HIV-1 Protease Inhibitors Encompassing a Tertiary Alcohol in the Transition-State Mimicking Scaffold[Show abstract] [Hide abstract]
ABSTRACT: Two series of P1'-extended HIV-1 protease inhibitors comprising a tertiary alcohol in the transition-state mimic exhibiting Ki values ranging from 2.1 to 93 nM have been synthesized. Microwave-accelerated palladium-catalyzed cross-couplings were utilized to rapidly optimize the P1' side chain. High cellular antiviral potencies were encountered when the P1' benzyl group was elongated with a 3- or 4-pyridyl substituent (EC50 = 0.18-0.22 microM). X-ray crystallographic data were obtained for three inhibitors cocrystallized with the enzyme.
Article: A New Class of HIV-1 Protease Inhibitors Containing a Tertiary Alcohol in the Transition-State Mimicking Scaffold[Show abstract] [Hide abstract]
ABSTRACT: Novel HIV-1 protease inhibitors encompassing a tertiary alcohol as part of the transition-state mimicking unit have been synthesized. Variation of the P1'-P3' residues and alteration of the tertiary alcohol absolute stereochemistry afforded 10 inhibitors. High potencies for the compounds with (S)-configuration at the carbon carrying the tertiary hydroxyl group were achieved with Ki values down to 2.4 nM. X-ray crystallographic data for a representative compound in complex with HIV-1 protease are presented.
Article: Structure of an Atypical Epoxide Hydrolase from Mycobacterium tuberculosis Gives Insights into its Function[Show abstract] [Hide abstract]
ABSTRACT: Epoxide hydrolases are vital to many organisms by virtue of their roles in detoxification, metabolism and processing of signaling molecules. The Mycobacterium tuberculosis genome encodes an unusually large number of epoxide hydrolases, suggesting that they might be of particular importance to these bacteria. We report here the first structure of an epoxide hydrolase from M.tuberculosis, solved to a resolution of 2.5 A using single-wavelength anomalous dispersion (SAD) from a selenomethionine-substituted protein. The enzyme features a deep active-site pocket created by the packing of three helices onto a curved six-stranded beta-sheet. This structure is similar to a previously described limonene-1,2-epoxide hydrolase from Rhodococcus erythropolis and unlike the alpha/beta-hydrolase fold typical of mammalian epoxide hydrolases (EH). A number of changes in the mycobacterial enzyme create a wider and deeper substrate-binding pocket than is found in its Rhodococcus homologue. Interestingly, each structure contains a different type of endogenous ligand of unknown origin bound in its active site. As a consequence of its wider substrate-binding pocket, the mycobacterial EH is capable of hydrolyzing long or bulky lipophilic epoxides such as 10,11-epoxystearic acid and cholesterol 5,6-oxide at appreciable rates, suggesting that similar compound(s) will serve as its physiological substrate(s).
Article: Rv0216, a conserved hypothetical protein from Mycobacterium tuberculosis that is essential for bacterial survival during infection, has a double hotdog fold[Show abstract] [Hide abstract]
ABSTRACT: The Mycobacterium tuberculosis genome contains about 4000 genes, of which approximately a third code for proteins of unknown function or are classified as conserved hypothetical proteins. We have determined the three-dimensional structure of one of these, the rv0216 gene product, which has been shown to be essential for M. tuberculosis growth in vivo. The structure exhibits the greatest similarity to bacterial and eukaryotic hydratases that catalyse the R-specific hydration of 2-enoyl coenzyme A. However, only part of the catalytic machinery is conserved in Rv0216 and it showed no activity for the substrate crotonyl-CoA. The structure of Rv0216 allows us to assign new functional annotations to a family of seven other M. tuberculosis proteins, a number if which are essential for bacterial survival during infection and growth.
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ABSTRACT: Carbonic anhydrases catalyze the reversible hydration of carbon dioxide to form bicarbonate. This activity is universally required for fatty acid biosynthesis as well as for the production of a number of small molecules, pH homeostasis, and other functions. At least three different carbonic anhydrase families are known to exist, of which the alpha-class found in humans has been studied in most detail. In the present work, we describe the structures of two of the three beta-class carbonic anhydrases that have been identified in Mycobacterium tuberculosis, i.e. Rv1284 and Rv3588c. Both structures were solved by molecular replacement and then refined to resolutions of 2.0 and 1.75 A, respectively. The active site of Rv1284 is small and almost completely shielded from solvent, whereas that of Rv3588c is larger and quite open to solution. Differences in coordination of the active site metal are also observed. In Rv3588c, an aspartic acid side chain displaces a water molecule and coordinates directly to the zinc ion, thereby closing the zinc coordination sphere and breaking the salt link to a nearby arginine that is a feature of Rv1284. The two carbonic anhydrases thus exhibit both of the metal coordination geometries that have previously been observed for structures in this family. Activity studies demonstrate that Rv3588c is a completely functional carbonic anhydrase. The apparent lack of activity of Rv1284 in the present assay system is likely exacerbated by the observed depletion of zinc in the preparation.
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ABSTRACT: HIV-1 protease is a pivotal enzyme in the later stages of the viral life cycle which is responsible for the processing and maturation of the virus particle into an infectious virion. As such, HIV-1 protease has become an important target for the treatment of AIDS, and efficient drugs have been developed. However, negative side effects and fast emerging resistance to the current drugs have necessitated the development of novel chemical entities in order to exploit different pharmacokinetic properties as well as new interaction patterns. We have used X-ray crystallography to decipher the structure-activity relationship of fluoro-substitution as a strategy to improve the antiviral activity and the protease inhibition of C2-symmetric diol-based inhibitors. In total we present six protease-inhibitor complexes at 1.8-2.3 A resolution, which have been structurally characterized with respect to their antiviral and inhibitory activities, in order to evaluate the effects of different fluoro-substitutions. These C2-symmetric inhibitors comprise mono- and difluoro-substituted benzyloxy side groups in P1/P1' and indanoleamine side groups in P2/P2'. The ortho- and meta-fluorinated P1/P1'-benzyloxy side groups proved to have the most cytopathogenic effects compared with the nonsubstituted analog and related C2-symmetric diol-based inhibitors. The different fluoro-substitutions are well accommodated in the protease S1/S1' subsites, as observed by an increase in favorable Van der Waals contacts and surface area buried by the inhibitors. These data will be used in the development of potent inhibitors with different pharmacokinetic profiles towards resistant protease mutants.
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ABSTRACT: Peptidyl-prolyl cis-trans isomerases (EC 22.214.171.124) catalyse the interconversion of cis and trans peptide bonds and are therefore considered to be important for protein folding. They are also thought to participate in processes such as signalling, cell surface recognition, chaperoning and heat-shock response. Here we report the soluble expression of recombinant Mycobacterium tuberculosis peptidyl-prolyl cis-trans isomerase PpiA in Escherichia coli, together with an investigation of its structure and biochemical properties. The protein was shown to be active in a spectrophotometric assay, with an estimated kcat/Km of 2.0 x 10(6) m(-1).s(-1). The X-ray structure of PpiA was solved by molecular replacement, and refined to a resolution of 2.6 A with R and Rfree values of 21.3% and 22.9%, respectively. Comparisons to known structures show that the PpiA represents a slight variation on the peptidyl-prolyl cis-trans isomerase fold, previously not represented in the Protein Data Bank. Inspection of the active site suggests that specificity for substrates and cyclosporin A will be similar to that found for most other enzymes of this structural family. Comparison to the sequence of the second M. tuberculosis enzyme, PpiB, suggests that binding of peptide substrates as well as cyclosporin A may differ in that case.
Article: Mycobacterium tuberculosis Ribose-5-phosphate Isomerase has a Known Fold, but a Novel Active Site[Show abstract] [Hide abstract]
ABSTRACT: Ribose-5-phosphate isomerases (EC 22.214.171.124) inter-convert ribose-5-phosphate and ribulose-5-phosphate. This reaction allows the synthesis of ribose from other sugars, as well a means for salvage of carbohydrates after nucleotide breakdown. Two unrelated types of enzyme are known to catalyze the isomerization. The most common one, RpiA, is present in almost all organisms. The second type, RpiB, is found in many bacterial species.Here, we demonstrate that the RpiB from Mycobacterium tuberculosis (Rv2465c) has catalytic properties very similar to those previously reported for the Escherichia coli RpiB enzyme. Further, we report the structure of the mycobacterial enzyme, solved by molecular replacement and refined to 1.88A resolution. Comparison with the E.coli structure shows that there are important differences in the two active sites, including a change in the position and nature of the catalytic base. Sequence comparisons reveal that the M.tuberculosis and E.coli RpiB enzymes are in fact representative of two distinct sub-families. The mycobacterial enzyme represents a type found only in actinobacteria, while the enzyme from E.coli is typical of that seen in many other bacterial proteomes. Both RpiBs are very different from RpiA in structure as well as in the construction of the active site. Docking studies allow additional insights into the reactions of all three enzymes, and show that many features of the mechanism are preserved despite the different catalytic components.
Article: Phenotypic drug susceptibility testing on HIV-1 reverse transcriptase recovered from human plasma[Show abstract] [Hide abstract]
ABSTRACT: To demonstrate the use of HIV-1 reverse transcriptase (RT) recovered directly from plasma for phenotypic drug susceptibility testing. Plasma from HIV-1 infected individuals with and without drug resistance-associated mutations were selected for the study. The blind coded plasmas were treated to inactivate cellular enzymes. The virions were immobilized on a gel and washed to remove antiretroviral drugs and RT activity blocking antibodies. The immobilized virions were lysed; the viral RT eluted and quantified, all according to the ExaVir Load procedure. The drug sensitivity profiles of each RT were determined using serially diluted drugs and modified Cavidi HS Lenti RT kits. The phenotypic drug sensitivity profiles of the RT and the patterns of drug resistance mutations were highly concordant. Plasma RT from virions devoid of mutations associated with drug resistance had average 50% inhibitory concentrations (IC(50)) of 1.5 +/- 0.93 microM for nevirapine, 0.21 +/- 0.099 microM for efavirenz, 7.1 +/- 3.2 microM for delavirdine, 0.42 +/- 0.15 microM for azidothymidine triphosphate and 0.059 +/- 0.018 microM for didehydrothymidine triphosphate. The increase in IC(50) value for RT with drug resistance associated substitutions was from 3- to more than 65-fold for non-nucleoside inhibitors and between 2- and 30-fold for thymidine analogue drugs. RT derived from virions recovered from the plasma of HIV infected individuals can be used for analysis of phenotypic drug susceptibility. The methods presented provide rapid alternatives for analysing phenotypic drug susceptibility especially when the therapy is based on non-nucleoside RT inhibitors and thymidine-analogue drugs.
Article: Evidence for a Mechanism of Recombination during Reverse Transcription Dependent on the Structure of the Acceptor RNA[Show abstract] [Hide abstract]
ABSTRACT: Genetic recombination is a major force driving retroviral evolution. In retroviruses, recombination proceeds mostly through copy choice during reverse transcription. Using a reconstituted in vitro system, we have studied the mechanism of strand transfer on a major recombination hot spot we previously identified within the genome of HIV-1. We show that on this model sequence the frequency of copy choice is strongly influenced by the folding of the RNA template, namely by the presence of a stable hairpin. This structure must be specifically present on the acceptor template. We previously proposed that strand transfer follows a two-step process: docking of the nascent DNA onto the acceptor RNA and strand invasion. The frequency of recombination under copy choice conditions was not dependent on the concentration of the acceptor RNA, in contrast with strand transfer occurring at strong arrests of reverse transcription. During copy choice strand transfer, the docking step is not rate limiting. We propose that the hairpin present on the acceptor RNA could mediate strand transfer following a mechanism reminiscent of branch migration during DNA recombination.
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ABSTRACT: HIV-1 protease is an important target for treatment of AIDS, and efficient drugs have been developed. However, the resistance and negative side effects of the current drugs has necessitated the development of new compounds with different binding patterns. In this study, nine C-terminally duplicated HIV-1 protease inhibitors were cocrystallised with the enzyme, the crystal structures analysed at 1.8-2.3 A resolution, and the inhibitory activity of the compounds characterized in order to evaluate the effects of the individual modifications. These compounds comprise two central hydroxy groups that mimic the geminal hydroxy groups of a cleavage-reaction intermediate. One of the hydroxy groups is located between the delta-oxygen atoms of the two catalytic aspartic acid residues, and the other in the gauche position relative to the first. The asymmetric binding of the two central inhibitory hydroxyls induced a small deviation from exact C2 symmetry in the whole enzyme-inhibitor complex. The study shows that the protease molecule could accommodate its structure to different sizes of the P2/P2' groups. The structural alterations were, however, relatively conservative and limited. The binding capacity of the S3/S3' sites was exploited by elongation of the compounds with groups in the P3/P3' positions or by extension of the P1/P1' groups. Furthermore, water molecules were shown to be important binding links between the protease and the inhibitors. This study produced a number of inhibitors with Ki values in the 100 picomolar range.
Article: Design and synthesis of HIV-1 protease inhibitors. Novel tetrahydrofuran P2/P2 '-groups interacting with Asp29/30 of the HIV-1 protease. Determination of binding from X-ray crystal structure of inhibitor protease complex[Show abstract] [Hide abstract]
ABSTRACT: A series of HIV-1 protease inhibitors having new tetrahydrofuran P2/P2' groups have been synthesised and tested for protease inhibition and antiviral activity. Six novel 4-aminotetrahydrofuran derivatives were prepared starting from commercially available isopropylidene-alpha-D-xylofuranose yielding six symmetrical and six unsymmetrical inhibitors. Promising sub nanomolar HIV-1 protease inhibitory activities were obtained. The X-ray crystal structure of the most potent inhibitor (23, K(i) 0.25 nM) co-crystallised with HIV-1 protease is discussed and the binding compared with inhibitors 1a and 1b.
Article: Structural basis for the inhibitory efficacy of efavirenz (DMP-266), MSC194 and PNU142721 towards the HIV-1 RT K103N mutant[Show abstract] [Hide abstract]
ABSTRACT: The K103N substitution is a frequently observed HIV-1 RT mutation in patients who do not respond to combination-therapy. The drugs Efavirenz, MSC194 and PNU142721 belong to the recent generation of NNRTIs characterized by an improved resistance profile to the most common single point mutations within HIV-1 RT, including the K103N mutation. In the present study we present structural observations from Efavirenz in complex with wild-type protein and the K103N mutant and PNU142721 and MSC194 in complex with the K103N mutant. The structures unanimously indicate that the K103N substitution induces only minor positional adjustments of the three inhibitors and the residues lining the binding pocket. Thus, compared to the corresponding wild-type structures, these inhibitors bind to the mutant in a conservative mode rather than through major rearrangements. The structures implicate that the reduced inhibitory efficacy should be attributed to the changes in the chemical environment in the vicinity of the substituted N103 residue. This is supported by changes in hydrophobic and electrostatic interactions to the inhibitors between wild-type and K103N mutant complexes. These potent inhibitors accommodate to the K103N mutation by forming new interactions to the N103 side chain. Our results are consistent with the proposal by Hsiou et al. [Hsiou, Y., Ding, J., Das, K., Clark, A.D. Jr, Boyer, P.L., Lewi, P., Janssen, P.A., Kleim, J.P., Rosner, M., Hughes, S.H. & Arnold, E. (2001) J. Mol. Biol.309, 437–445] that inhibitors with good activity against the K103N mutant would be expected to have favorable interactions with the mutant asparagines side chain, thereby compensating for resistance caused by stabilization of the mutant enzyme due to a hydrogen-bond network involving the N103 and Y188 side chains.
Top Journals
Uppsala University
Uppsala, Uppsala, Sweden
- Department of Cell and Molecular Biology
Max Planck Institute of Molecular PhysiologyDortmund, North Rhine-Westphalia, Germany
BMC SoftwareHouston, Texas, United States


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bus tweets
email: firstname.lastname@example.com
Recent Posts
- Remembering why I ride
- On buses and boundaries
- Art + buses + community = life (part II)
- Respect to those who came before, part V (Or, Why we need Indigenous People's Day)
- On cars and community
- Buses are for everyone, part IV
- Multimodal Monday: Greenway riders
- Power to the people
- Art + buses + community = life
- A beautiful, brief ride
In the Bus Bag
Spiritual Ecology: The Cry of the Earth, edited by Llewellyn Vaughn-Lee
Search Results for: "bus chick high season"
How the Bus Fam celebrates a sunny day
I am not a fair weather bus chick. I love my city (rain, clouds, and late-spring chill included) and my carfree life no matter the season. I'd be lying, though, if I said that I didn't prefer getting around during time of year when it's light both early and late and there's a high probability of sunshine.
During the months between May and October (aka, bus chick high season) life on the ground is lovely–far, far prefarable to life trapped inside an exhaust-spewing metal box stuck in baseball traffic. We walk more than we wait (truth be …
10:37 pm Comments (1)at
Category: busing with babies, living the life, reasons to ride Tagged 27, 56, Alki, caroline, Tahoma, Water Taxi, West Seattle
Category: busing with babies, living the life, reasons to ride Tagged 27, 56, Alki, caroline, Tahoma, Water Taxi, West Seattle
Fellow riders, take heart
We have rounded the bend!
I know the photograph ain't much (yet again, the bus was coming), so in case you can't tell: It's a cherry tree (near the westbound 27 stop at Yesler & Broadway), sporting actual buds.
Only 83 days until the beginning of bus chick high season.
10:54 pm Comments Off on Fellow riders, take heartat
Category: spotted! Tagged high season for bus chicks, Spotted!
Category: spotted! Tagged high season for bus chicks, Spotted!
On this, the last day of summer (known to some as the end of the bus chick high season), I came across a minor bus/truck fender bender in Pioneer Square. Luckily, the bus was headed to base, so no passengers were displaced–unless, that is, I got there after the riders had already been booted. If they were, I'm sure it only made them stronger. You haven't earned your true bus chick stripes until you've been kicked off a "disabled" bus in the rain.


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Our daughter Lydia attends a public Waldorf school. The principal begged parents last year to make their Christmas gifts, saying, "If you make a doll, you've taught your child that she has the resources to make what will please her. If you buy her a remote control race car, you've taught her that entertainment is expensive and supplied by a corporation."
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Scattered Trust
Fantasy, reality,
Borders fade between the two.
Where is the line
Between false and true?
Like an eternal, icy flame;
It has a solid grip
On your wary mind,
Always lurking in your thoughts,
As you wonder
If you can truly tell the difference,
Or if you feed on lies
And false truths.
Once burned
By the shearing hand of betrayal
Your trust is lost
And must be found
Once more,
Brought back from the
Dark abyss that is your fear;
Fear of breaking,
Fear of losing everything you know.
But how can you learn to trust
When the raw pain of heartache
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