text:
Watch 540 dancing robots celebrate Chinese New Year
There's also drones making it rain glitter.
Foxconn likely to become Sharp's new owner
The firm that manufactures the iPhone is putting a $5.5 billion bet on being able to rescue Sharp.
Two major visual effects studios join forces for VR push
The animators behind 'Transformers' acquire their Chinese counterpart.
Tesla wants a factory in China, and fast
Model X will be headed to Hong Kong as well later this year.
The US is looking into exploding self-balancing skateboards
America's CPSC has launched a priority investigation into the devices after a spate of explosions.
China's Twitter will expand text limit to 2,000 characters
Weibo is launching expanded messages as soon as February.
China plans to visit the dark side of the moon in 2018
We've got a music recommendation for the control room.
Has the 'Bitcoin experiment' failed?
Prominent developer exits the community, saying the cryptocurrency is a failed experiment.
Amazon is getting into the oceanic freight shipping game
It's more exciting than it sounds. Really.


text:
He had one of the best TV character names of all time. No, not that one. The other one – "Bob Loblaw."
One of the questions I frequently ask in the Dozen is "What cancelled show would you put back on the air?" For me, it would definitely be the television home of Bob Loblaw, Arrested Development. I'm excited that I had the opportunity to interview one of my favorite actors from the show, Scott Baio.
Baio is best known for his starring roles in popular sitcoms, including Happy Days, Joanie Loves Chachi and Charles in Charge, as well as two seasons on VH1's Scott Baio is 45… and Single. After the end of the first season, his then-girlfriend now wife, Renee, announced that she was pregnant, which lead to a name-change for the show – Scott Baio is 46… and Pregnant.
Following the birth of their daughter, Bailey, they started the Bailey Baio Angel Foundation to build awareness for newborn screenings for organic acidemia metabolic disorders. They also raise funds to help support families and children affected by these disorders. (For more information and to donate, visit www.baileybaioangelfoundation.com).
Baio is a conservative Republican who often tweets funny cartoons and observations. A couple of them have raised the ire of the Left. After tweeting an unflattering photo of Michelle Obama, people accused him of being racist and issued death threats against him and his family.
By contrast, this week the tolerant Left showed their respect for the office of the Presidency. The President invited several gay leftists to the White House to recognize their leadership in the gay community. They took it as an opportunity to post photos of themselves flipping off portraits of Presidents Reagan and George W. Bush. These so-called leaders behaved like immature teenagers on Facebook. (My apologies to teenagers for the comparison).
Those in Hollywood frequently like to pat one another on the back and call themselves brave for speaking out against Republican leaders, America or even the military. However, real bravery in Hollywood is being a conservative. Last year Baio was a guest speaker at the Hollywood Congress of Republicans meeting. He told the crowd, "I was never afraid to speak my mind. But I do remember times on a set where I'd hear my liberal friends talking and I didn't speak out. I stepped back and did not comment because there is a stigma [within the industry] that if you talk [as a conservative], you don't work."
Be the first to read Lisa De Pasquale's column. Sign up today and receive Townhall.com delivered each morning to your inbox.
Bush mocks Rubio and his fans: Stop whining that it's "unfair" that you're being attacked by another candidate's Super PAC | Allahpundit

subset: commoncrawl


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The Kansas Firefighters Museum has coordinated a free fire camp for graduating 8th graders. We could use your help in getting this information out to the public. This is our 1st Annual Kansas Firefighters Museum Fire Camp designed to give students a glimpse into the world of firefighting.
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Burt's Bees Rosemary Mint Shampoo Bar [DISCONTINUED]
81 reviews
33% would repurchase
Package Quality: 2.9
Price: $$
Package Quality: 2.9
Price: $$
Not tested on animals
Burt's Bees is a Cruelty-Free Subsidiary.
INGREDIENTSVegetable soap base, rosmarinus officinalis (rosemary) leaf oil, yucca vera extract, avena sativa (oat) kernel protein, urtica dioca (nettle) leaf powder, persea gratissima (avocado) oil, mentha piperita (peppermint) oil, rosmarinus officinalis (rosemary) leaf powder, cocos mucifera (coconut) oil, coconut acid.
Age: 30-35
Skin: Combination, Other, Not Sure
Hair: Blond, Other, Other
Eyes: Blue
I've used JR Leggets shampoo bar and found it effective, but a little drying. I was hoping this woud be gentler and that the peppermint would be refreshing in the morning. It was and rubbing it directly on my scalp banished a nagging case of soreness from an overly tight ponytail.
However, it didn't wash out the BB's Avacado Butter Hair Treatment (which I love). This left my hair greasy and stringy. I tried washing my hair again with the bar and my hair was cleaner, but dry and coated with film.
This would be a good environmentally friendly item for a camping trip (where beauty is secondary), but I wouldn't recommend it for everyday.
Age: 19-24
Skin: Combination, Other, Not Sure
Hair: Brown, Other, Other
Eyes: Blue
This is an absolutely dreadful shampoo in every sense-- it makes my hair dry and yet leaves a heavy build-up, it's difficult to work through my hair and difficult to store because it comes in bar form. However, when I'm between cleansers, I use it as a facial soap and it works very, very nicely in that capacity! (Although does not remove make-up.)
Age: 25-29
Skin: Very Dry, Other, Not Sure
Hair: Blond, Other, Other
Eyes: Blue
I really enjoy this as a shampoo and as a body cleanser. It has a strong scent, that is natural smelling. A little goes a long way. Great for oily hair/scalp. Others seem to dislike this, but I love it, so to each her own. I would buy this again, it's great.
Age: 36-43
Skin: Normal, Other, Not Sure
Hair: Brunette, Other, Other
Eyes: Hazel
I would definitely not use this on my hair, no way. I've heard it's very drying and bar soap forms of shampoo never really grabbed me. However, as a body soap I might reconsider, being that I love the smell and idea of this. Don't know if my initial impressions however, would score a repurchase. Luckily, I tried this in the trial pack size and would recommend anyone who's curious about this line to get the trial pack first. Burt's Bees take some getting used to for those of us who are used to pretty packaging and fancier products! Jury 's still out on this one.
Age: 25-29
Skin: Sensitive, Fair-Medium, Neutral
Hair: Brunette, Wavy, Medium
Eyes: Hazel
I know it's mean but I just have to laugh at anyone who really thought that a bar SOAP would be better than an actual shampoo- yes, Burt, I am laughing at YOU! This stuff is crap; and I mean crap. It's drying and the actual bar itself gets caught and tangled in hair! There must have been a cannibis scented soap nearby when this was formulated.
Maybe I'll use this stuff to bathe my cat.
Age: 25-29
Skin: Sensitive, Fair, Cool
Hair: Brunette, Wavy, Fine
Eyes: Hazel
I got this in the Burt's Bees sampler pack. A shampoo bar sounds like a neat idea, but this stuff is no good! It felt very drying in my hair, and made it a tangled mess in the shower, plus it took like 15 minutes to rinse out. Not so good for those with hair longer than an inch. No thanks, Burt.
Age: 19-24
Skin: Combination, Fair, Neutral
Hair: Brunette, Wavy, Fine
Eyes: Hazel
my bf loves this too, but i hate it on my hair, it was super dry and tangled after i used this. so i guess its ok for people with really short hair (mine is chin length)
Age: 19-24
Skin: Combination, Other, Not Sure
Hair: Black, Other, Other
Eyes: Black
My boyfriend LOVES it!!! He swears by it!!! It is a great Valentine gift to buy for a health-conscious guy, especially since sulfactants kill hair follicles. It helps hair to grow fuller.
Age: 25-29
Skin: Combination, Other, Not Sure
Hair: Black, Other, Other
Eyes: Brown
This dried my hair out and my hair didn't feel any cleaner after using it. It's also too easy to confuse it for your regular soap if it's a similar color.
Age: 30-35
Skin: Very Oily, Fair-Medium, Neutral
Hair: Brunette, Straight, Fine
Eyes: Grey
I was fully prepared to hate this product, seeing as I disliked almost everything else in the starter pack. It's hard to get onto your hair at first. You have to put it directly onto your hair--forget about trying to create a lather in your hands. However, it really cleaned my oily hair. Your hair will feel strange after rinsing, but a conditioner will help. I like the idea of a bar shampoo, but I would be more likely to buy it if there were a version that had a more feminine fragrance.
Popular Shampoos
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text:
Derrick Brooks Gets Quizzed On Super Bowl XXXVII
The Buccaneers linebacker had a big game when Tampa hammered the Raiders to win the title. How well does he fare
Kel Mitchell Recites 'Orange Soda Rap,' Reminisces On 'Kenan & Kel'
The Nickelodeon legend has a new show on the network. How 'All That' was written
Mean Joe Greene Reunites With Coca-Cola Commercial Kid
The Steelers great meets with his co-star in a classic ad. Who gets credit?
Throwback: 'Send It In, Jerome!'
Panthers star Jerome Lane unleashed one of the most memorable dunks in college basketball. Relive the moment
'Super Bowl Shuffle' Turns 30
The recording session takes place the day after the team's only loss of the season. Relive the magic
One Year Ago Today, OBJ Broke The Laws Of Physics
The Giants receiver's back-bending, fingertip grab might be the greatest of all-time. Birth of a star
Watch Stephen Curry, Blake Griffin Face Off In College
NBA superstars put on an instant classic seven years ago. Not much has changed
Hook'Em Horns Turns 60
When the Longhorns hosted TCU on Nov. 12, 1955, it was the first time that the famous hand sign was flashed at a Texas football game. Meet the creator
When Packers Considered Putting Dome On Lambeau
Green Bay has the ultimate home-field advantage, but back in the early 80s, the team considered following Minnesota and Detroit by going indoors. See the design
Flashing Back To '85 After Royals Finally Win Again
See what else was happening besides Michael Jordan being NBA rookie of the year. Tech developments


text:
An all-level class based on Ashtanga Vinyasa Flow; it initiates "breath to movement" awareness and emphasizes safe alignment to keep joints stable and muscles working in order to strengthen and increase flexibility. This flow will help you detoxify, heal and rejuvenate, as well as still your mind to leave you present, connected and on purpose. […]MORE INFO
A spiced up Vinyasa Flow that improves circulation, balance and mindfulness. This class will help you experience the many benefits of Power Vinyasa: detoxifying, burning calories and building a lean, firm and strong body. You will have the opportunity to encounter an overwhelming sense of empowerment as you build endurance, muscle and confidence. 60-75 Min […]MORE INFO
A practice that adapts to every individual's needs. Postures are approached in gradual steps, utilizing props for support and plenty of time for breathing. Great for people with movement limitation and new practitioners. 60 Min classMORE INFO
Yoga Therapy is for people who have injuries, chronic pain, or just want to stabilize muscles, joints and improve posture. Perfectly healthy people who want to create and maintain more balance, are welcome as well. This class is slow paced and healing and recommended for ALL Yogi's. as well as those who have never tried […]MORE INFO


text:
EnglishDeutschFrançais Español PortuguêsDansk SvenskaNederlands Ελληνικά Русский языкItali
World of Warcraft EU
World of Warcraft US
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Dark Runescape
Deadman Mode
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Diablo III EU
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Final Fantasy XI
Ever Quest 2
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Tera US
Rift Planes of Telara US
Rift Planes of Telara EU
Eden Eternal
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ZYY.com has passed the test and certification of McAfee, which is the most famous network secure vendor. This daily test will insure you a safety shopping
The Best PvP Class Guide in Rift
Should you be looking for the best PvP Rift class, then look no further than the Warrior calling. Whether you want to play solo or perhaps in groups, these kinds ought to be your top pick for PvP play. The rift gold is not useful in PvP class.
In particular, the Rift blade and Void Knight create a powerful combination that may beat any class in the game in a 1v1 match-up. It literally doesn't have weakness, despite a number of nerfs which have been made to get this to class easier to defeat.
This class is so strong since the Void Knight is an anti-caster specialist and the Riftblade is definitely an anti-armored specialist. Whenever you place the two classes together, you've got a class that takes little damage from physical and spell attacks, can drain mana, and do quite a lot of elemental damage.
It is the best class versus Clerics and Mages because it has a number of magic-damage shields and can drain mana while dealing quite a lot of damage. No class hanging around has the same degree of mana drains this ultimate Rift PvP calling has.
Additionally, the Riftblade class does great damage while using the plate along with a shield. This is a problem because not only does it result in the Warrior the anti-caster it causes it to be the anti-melee. Additionally, it allows Warriors to equip large amounts of stamina and mitigation gear.
Due to the decent, sustained damage creation of the Riftblade (despite recent nerfs), players simply are unable to compete with this class in a one-on-one match up. The Riftblade will easily complete a player well before the ball player has the capacity to put a dent in their massive HP pool.
Consequently, no class is capable of doing beating the Warrior in a 1-on-1 fight. This is exactly what makes the Warrior the best PvP class in Rift.
Many players prefer to say the Bard is overpowered and that it is the best class in PvP. The only reason someone could arrived at this type of conclusion is that if they have not yet literally game past level 19.
Sure, in the first tier of Warfronts (level 10-19), Cadence seems to be very, excellent. It allows Rogues to dish out decent damage while healing the whole group for 10% of their max healthy every 2-3 seconds.
However, fast-forward to level 40, and everyone's HP pool has grown by 3-4 times, however the damage of cadence has only doubled. Before you would obtain a 10% heal from Cadence and now you only obtain a 5% heal. By cutting this ability's effectiveness, the Bard's healing quickly goes from overpowered to hardly noticeable.
However, not everyone is a Warrior or wants to play one. Check out rift planes of telara guide net and check out articles featuring the very best Rift Cleric Builds or Rift Rogue builds for additional PvP builds and concepts, regardless of what your chosen calling is within Rift!
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Montrail Badwater Hybrid Shoe - Men'sView Larger Image
- Detail Images
If Mondays find you on the road, followed swiftly by Trail Tuesdays, then lace up the Montrail Badwater Hybrid Shoe before your next run. A multi-use design offers the versatility you need to transition seamlessly from pavement to pines.
- Variable Fit System features variable width lacing and seamless support frame for optimal medial support and a fit custom to your foot
- EVA foam specifically targets proven impact zone for both durability and additional cushioning
- Multi-surface outsole design and Gryptonite sole provide traction on a variety of surfaces
- Open mesh upper encourages ventilation


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GameWorks, GameWorks Expert Developer, Vulkan
Vulkan offers another key difference to OpenGL with respect to memory allocation. When it comes to managing memory allocations as well as assigning it to individual resources, the OpenGL driver does most of the work for the developer. This allows applications to be developed, tested and deployed very quickly.
Read more
GameWorks, GameWorks Expert Developer, NVIDIA Shield
2016 is going to be a very exciting year for NVIDIA at GDC. We'll be demonstrating the most advanced real-time rendering for VR and video games, as well as the NVIDIA SHIELD platform.
Between our booth, developer labs, presentations, meetings, and other activities, this is going to be our biggest, most exciting GDC yet.
We can't wait to see you at the show!
In this blog post we will go into further details of one of the most common state changes in scene rendering: binding shader resources such as uniform- or storage-buffers, images or samplers.
GameWorks, Android, Developer Tools, CodeWorks, Nsight Visual Studio Edition, DirectX 12, Linux Graphics Debugger, Nsight Tegra Visual Studio Edition, Tegra Graphics Debugger, Tegra System Profiler, PerfKit
The latest NVIDIA GameWorks developer tools helps you optimize your applications for a variety of platforms. Both desktop and mobile developer tools have updated releases with many new features – check them out!
Many of this year's most exciting PC games feature GameWorks technologies. We worked closely with developers to put ten different GameWorks
modules including HBAO+, HairWorks and FleX into more than a dozen games.
These high-octane titles feature amazing particles, realistic hair, and cinematic post processing to rival Hollywood.


text:
Format: PC, PS3, iOS
Release Date: October 2009, Q4 2012(PS3)
Publisher: Amanita Design
Developer: Amanita Design
Price: $9.99
ESRB Rating: E
Machinarium has to be one of the more special indie games ever released. With its imminent release for PS3, I had to go back and finish the PC version of the game, and I loved every minute of it.
Presentation and Story:
You play as young Josef, a small robot that gets exiled to the junkyard. An evil corporation, the Black Cap Brotherhood, has taken over his city and he must get it back. Josef is also going into Machinarium to rescue his robot girlfriend from the evil BCB as well. Its a charming story, to say the least, even though its pretty simplistic in its limited scope. The one thing that takes this game to the next level, has to be its sublime 2D visuals. Everything in the world looks hand painted and hand crafted from the ground up. I used to call games cutesy, but I now know that developers don't particularly approve of anybody calling their games cute, but I digress. The word charming will come up frequently when discussing this game. The robots themselves show and possess a lot of emotion, even though there isn't a single lick of dialogue in the game. You'll come across robots expressing deep levels of boredom, frustration and even a bit of anxiety. Surrounding these robots are a number of highly detailed environments that really engrosses you into this depressing but quirky world. Since there isn't any dialogue in the game besides the random sound effects certain robots exude, the mechanical soundtrack definitely adds what the lack of voice takes away.
Core Gameplay:
The gameplay is very simple, but a number of challenging puzzles will keep you engaged throughout your playthrough. Its a simple point and click adventure that really won't take you that long to finish. If you're not as apt to solve some of the more daunting puzzles, like myself, then the game utilizes a cool intuitive hint system. Clicking a certain button will display a visual hint for what you need to do, if this still doesn't help you in any way, you hit that button again and the game will show you a step-by-step visual guide needed to solve the puzzles. Its a bit of a cop out, but even though it tells you the steps, it doesn't show you everything you need to know, so it'll still require some brainpower to actually finish the game. You won't be able to just randomly click across the entire screen to find what you need to advance, the game's excellent level design makes that impossible. Any puzzle fan will come to appreciate the game's challenging yet fair puzzles and its intuitive puzzle-solving mechanics as well.
Final Thoughts:
Even though the game isn't a long by any means, for $10 bucks, you're getting a quality indie title, which is very hard to come by nowadays. Some truly mesmerizing visuals and great puzzles to complete, this one was an easy purchase.
S&S Rating: 8.5/10


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Need to Know: What to look for at today's OTA practice including RG3's progress and who's playing strong safety.
Although nothing has been announced as of now, it looks like the reported joint practices between the Redskins and Houston Texans are on.
Last year, the Washington Redskins held joint practices with the New England Patriots at Bon Secours Training Center in Richmond, Virginia.
Three reasons why signing Matt Schaub made sense for the Ravens:
1. The Ravens can't expect Joe Flacco's luck to last forever.
The Ravens signed quarterback Matt Schaub to a one-year deal worth $3 million.


text:
Amidst a flurry of interest by newspaper and magazine publishers in e-books as a new revenue stream, the biggest player in e-books–Amazon–isn't resting on its laurels.
The Seattle-based e-commerce company on Monday morning said it is holding another press conference Wednesday in New York, the scene of past announcements about its Kindle e-reader. The event will once again be hosted by the company's chief executive (and chief e-book enthusiast) Jeff Bezos.
Amazon hasn't said what the event is about, but the periodicals industry is buzzing with anticipation for the announcement of a larger Kindle. As the Journal reported on April 11, Amazon has been preparing a larger-screen Kindle for some time. Such a device could have several advantages, including better fitting the size and layout of newspaper pages (including advertising) and academic textbooks, which often feature large charts and diagrams.
A story in the New York Times on Monday mentioned that newspapers including its own could be involved in a Kindle announcement as soon as this week.
The press conference will be held at Pace University in New York. Our colleagues at All Things Digital point out that Pace is located on the site of the New York Times's 19th century headquarters.
For the latest news and analysis, follow @wsjd.
And like us on Facebook to get our news right in your feed:
Get breaking news and personal-tech reviews delivered right to your inbox.
More from WSJ.D: And make sure to visit WSJ.D for all of our news, personal tech coverage, analysis and more, and add our XML feed to your favorite reader.


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Are you Matti Sarvas?Claim your profile
Publications (97)295.37 Total impact
- [Show abstract] [Hide abstract]
ABSTRACT: A gram-negative obligate intracellular bacterium, Chlamydia pneumoniae, is a common respiratory pathogen. Here, we examined the invasion and attachment of C. pneumoniae K6 into nonphagocytic HL epithelial cell line by manipulating host plasma membranes by using cholesterol-depleting methyl-beta-cyclodextrin (MβCD) and cholesterol-loading MβCD complexed cholesterol (chol-MβCD). The invasion was attenuated by MβCD-treatment while chol-MβCD augmented the attachment and invasion. In addition, the invasion was inhibited by cholesterol sequestering reagents, nystatin and filipin. Furthermore, exposure of host cells to sphingomyelinase inhibited the invasion. RNA interference was used to assay the role of clathrin and human scavenger receptor B, type I (SR-BI) in the entry of C. pneumoniae into A549 lung epithelial adenocarcinoma cells. In contrast to Chlamydia trachomatis L2, the entry of C. pneumoniae was found to be independent of clathrin. In addition, the entry was found to be SR-BI-independent, but interestingly, the chlamydial growth was attenuated in the SR-BI-silenced cells. These findings suggest that the attachment and invasion of C. pneumoniae into nonphagocytic epithelial cells is dependent on the formation of cholesterol- and sphingomyelin-rich plasma membrane microdomains, and the entry is a clathrin-independent process. In addition, our data indicate that SR-BI supports the growth of C. pneumoniae in epithelial cells.
Article: The density of negative charge in the cell wall influences two-component signal transduction in Bacillus subtilis[Show abstract] [Hide abstract]
ABSTRACT: The Dlt system modulates the density of negative charge in the cell wall of Gram-positive bacteria by substituting anionic polymers (wall and lipoteichoic acids) with d-alanine. The htrA and htrB genes, regulated by the CssRS two-component system (TCS) and encoding membrane-associated protein quality control proteases, were expressed at strongly decreased levels in a mutant with defective Dlt (dltD : : miniTn10) as compared to the dlt(+) wild-type strain under a secretion stress condition (hypersecretion of AmyQ alpha-amylase). The level of HtrA protein in the extracellular proteome of the dltD mutant was decreased consistently. Expression from the promoter of the liaIHGFSR (yvqIHGFEC) operon (P(liaI)) is dependent on the LiaRS TCS. The Dlt defect increased the expression from P(liaI) under two stress conditions, AmyQ hypersecretion and treatment with a cationic antimicrobial peptide (LL-37), but decreased the expression in vancomycin-treated cells. Furthermore, Dlt inactivation enhanced the expression of the YxdJK-regulated yxdL gene in LL-37-treated cells. The increased net negative charge of the cell wall seems to cause varied and opposite effects on the expression of CssRS-, LiaRS- and YxdJK-regulated genes under different stress conditions. The results suggest that TCSs which sense misfolded proteins or peptides are modulated by the density of negative charge in the cell wall. The density of negative charge on the outer surface of the cell membrane did not have a similar effect on TCSs.
Article: Intranasal administration of chlamydial outer protein N (CopN) induces protection against pulmonary Chlamydia pneumoniae infection in a mouse model[Show abstract] [Hide abstract]
ABSTRACT: Chlamydia pneumoniae is an intracellular pathogen that grows inside a vacuole, referred to as an inclusion. C. pneumoniae possess a type III secretion system (TTSS), which allows them to secrete effector molecules into the inclusion membrane and to the host cell cytosol. Proteins such as chlamydial outer protein N (CopN) that associate with the inclusion membrane are potential targets for the host's MHC-dependent antigen presentation, thereby representing ideal antigen candidates for T cell-based vaccination. The results of this study showed that intranasal immunization of BALB/c mice with heat-aggregated CopN protein and an Escherichia coli heat-labile toxin (LT) induced a strong immune response, detected as antigen-specific antibody production, lymphocyte proliferation and IFN-gamma production. Furthermore, the immunization induced statistically significant protection against intranasal C. pneumoniae challenge, the level of which correlated with the magnitude of CopN-specific lymphocyte proliferation. Both heat-aggregation of the antigen and the presence of LT adjuvant were required for maximal protective effect.
Article: Systemic and mucosal antibody response in experimental Chlamydia pneumoniae infection of mice[Show abstract] [Hide abstract]
ABSTRACT: Chlamydia pneumoniae is a common human respiratory pathogen, and sera from infected individuals recognize several proteins of C. pneumoniae. We produced C. pneumoniae-specific proteins in a Bacillus subtilis expression system. We then used these recombinant C. pneumoniae proteins and purified C. pneumoniae elementary bodies as antigens in enzyme immunoassays to assess the kinetics and protein specificity of the systemic and mucosal antibody responses induced by C. pneumoniae intranasal infection in BALB/c mice. The systemic antibodies in mice recognized strong 'key' immunogens of Chlamydia, Omp2 and Hsp60, but weakly targeted the MOMP protein, the major immunogen in chlamydial species other than C. pneumoniae. The IgA antibodies in bronchial secretions specifically recognized the putative surface protein of C. pneumoniae, Omp4. Our preliminary observations point to the necessity of further characterization of the mucosal antibody response during C. pneumoniae infection.
Article: NMR solution structure and characterization of substrate binding site of the PPIase domain of PrsA protein from[Show abstract] [Hide abstract]
ABSTRACT: PrsA is a peptidyl-prolyl isomerase (PPIase) from Bacillus subtilis belonging to the parvulin family of PPIases. It is a membrane bound lipoprotein at the membrane-wall interface, involved in folding of exported proteins. We present the NMR solution structure of the PPIase domain of PrsA, the first from a Gram-positive bacterium. In addition we mapped out the active site with NMR titration experiments. A high degree of conservation with other members of the parvulin family was revealed in the structure and binding site. Interactions with substrate peptides were also characterized by mutated domains revealing that H122 is indispensable for overall correct folding.
Article: Increased secretion of α-amylase from Bacillus subtilis caused by multiple copies of α-amylase gene from B. amyloliquefaciens is not further increased by genes enhancing the basic level of secretion
Article: P fimbriae of Escherichia coli: Molecular cloning of DNA fragments containing the structural genes
Article: Bacillus subtilis α-amylase: the rate limiting step of secretion is growth phase-independent[Show abstract] [Hide abstract]
ABSTRACT: When Bacillus subtilis-amylase was expressed under the control of sacR in a degU32(Hy) strain, the production of exoenzyme occurred during both the exponential and stationary phases of growth. In each phase, pulse-chase experiments showed that the rate-limiting step of the secretion process was the release of the processed form of the protein in each physiological context. The rate of this event was slightly slower (t1/2=3.2 min) during the stationary phase than during the exponential phase (t1/2=2 min). The effectors which possibly control the efficiency of the release stage, the level of PrsA or the calcium binding properties of the cell wall, remained unchanged throughout growth phases.
Article: Production of proteins in Bacillus subtilis can be improved by engineering components affecting posttranslocational protein folding and degradation
Article: Clearance of Chlamydia pneumoniae Infection in H-2 Class I-/- Human Leucocyte Antigen-A2.1 Monochain Transgenic Mice[Show abstract] [Hide abstract]
ABSTRACT: CD8+ T cells have been suggested to play an important role in protective immunity against pulmonary Chlamydia pneumoniae infection in mice. Moreover, several classical major histocompatibility complex class I - restricted cytotoxic CD8+ T lymphocytes (CTL) specific for C. pneumoniae- derived peptides have been identified. Here, we studied the outcome of C. pneumoniae infection in human leucocyte antigen (HLA)-A2.1 transgenic mice (HHD mice) that are only able to express a classical human class I molecule (HLA-A2.1). C. pneumoniae infection was self-restricted in HHD mice which were able to develop specific immune responses and a protective immunity against a subsequent rechallenge in a manner comparable to wildtype mice. Furthermore, accumulation of functional and C. pneumoniae-specific T cells to the site of infection was detected after challenge. Antigen processing and HLA-A2.1-dependent presentation was studied by immunizing the HHD mice with chlamydial outer protein N (CopN). Isolation of a peptide-specific CTL line from the CopN-immunized mice suggests that the HLA-A2.1 molecule can support the development of CTL response against a chlamydial protein in mice. These findings suggest that the transgenic mouse model can be used for further characterization of the HLA-A2.1-restricted CD8+ T-cell response during C. pneumoniae infection and for identification of CD8 epitopes from chlamydial antigens.
Article: Cationic antimicrobial peptides elicit a complex stress response in Bacillus subtilis that involves ECF-type σ factors and two-component signal transduction systems[Show abstract] [Hide abstract]
ABSTRACT: Stress responses of Bacillus subtilis to membrane-active cationic antimicrobial peptides were studied. Global analysis of gene expression by DNA macroarray showed that peptides at a subinhibitory concentration activated numerous genes. A prominent pattern was the activation of two extracytoplasmic function sigma factor regulons, SigW and SigM. Two natural antimicrobial peptides, LL-37 and PG-1, were weak activators of SigW regulon genes, whereas their synthetic analogue poly-L-lysine was clearly a stronger activator of SigW. It was demonstrated for the first time that LL-37 is a strong and specific activator of the YxdJK two-component systems, one of the three highly homologous two-component systems sensing antimicrobial compounds. YxdJK regulates the expression of the YxdLM ABC transporter. The LiaRS (YvqCE) TCS was also strongly activated by LL-37, but its activation is not LL-37 specific, as was demonstrated by its activation with PG-1 and Triton X-100. Other strongly LL-37-induced genes included yrhH and yhcGHI. Taken together, the responses to cationic antimicrobial peptides revealed highly complex regulatory patterns and induction of several signal transduction pathways. The results suggest significant overlap between different stress regulons and interdependence of signal transduction pathways mediating stress responses.
- [Show abstract] [Hide abstract]
ABSTRACT: Transcription profiling of all protein-encoding genes of Bacillus subtilis was carried out under several secretion stress conditions in the exponential growth phase. Cells that secreted AmyQ alpha-amylase at a high level were stressed only moderately: seven genes were induced, most significantly htrA and htrB, encoding quality control proteases, and yqxL, encoding a putative CorA-type Mg(2+) transporter. These three genes were induced more strongly by severe secretion stress (prsA3 mutant secreting AmyQ), suggesting that their expression responds to protein misfolding. In addition, 17 other genes were induced, including the liaIHGFSR (yvqIHGFEC) operon, csaA and ffh, encoding chaperones involved in the pretranslocational phase of secretion, and genes involved in cell wall synthesis/modification. Severe secretion stress caused downregulation of 23 genes, including the prsA paralogue yacD. Analysis of a cssS knockout mutant indicated that the absence of the CssRS two-component system, and consequently the absence of the HtrA and HtrB proteases, caused secretion stress. The results also suggest that the htrA and htrB genes comprise the CssRS regulon. B. subtilis cells respond to secretion/folding stress by various changes in gene expression, which can be seen as an attempt to combat the stress condition.
Article: Secretion of heterologous proteins in Bacillus Subtillis can be improved by engineering cell components affecting post-translocational protein folding and degradation[Show abstract] [Hide abstract]
ABSTRACT: To explore the potential to enhance secretion of heterologous proteins in Bacillus subtilis by engineering cell factors affecting extracytoplasmic protein folding and degradation. Bottleneck components affecting the extracytoplasmic phase of protein secretion were genetically engineered and their effects on the secretion of 11 industrially interesting heterologous proteins were studied by Western blotting and enzymatic assays. Overproduction of PrsA lipoprotein enhanced the secretion of alpha-amylase of Bacillus stearothermophilus (fourfold) and pneumolysin (1.5-fold). Increasing the net negative charge of the cell wall because of lack of the d-alanine substitution of anionic cell wall polymers enhanced the secretion of pneumolysin c. 1.5-fold. Decreasing the level of HtrA-type quality control proteases caused harmful effects on growth and did not enhance secretion. Pertussis toxin subunit, S1 was found to be a substrate for HtrA-type proteases and its secretion was dependent on these proteases. Secretion of heterologous proteins can be enhanced by engineering components involved in late stages of secretion in a protein-dependent manner. The study revealed both possibilities and limitations of modulating the post-translocational phase of secretion as a means to improve the yield of heterologous proteins.
- [Show abstract] [Hide abstract]
ABSTRACT: The transport of proteins from their site of synthesis in the cytoplasm to their functional location is an essential characteristic of all living cells. In Gram-positive bacteria the majority of proteins that are translocated across the cytoplasmic membrane are delivered to the membrane-cell wall interface in an essentially unfolded form. They must then be folded into their native configuration in an environment that is dominated by a high density of immobilised negative charge-in essence an ion exchange resin. It is essential to the viability of the cell that these proteins do not block the translocation machinery in the membrane, form illegitimate interactions with the cell wall or, through intermolecular interactions, form insoluble aggregates. Native Gram-positive proteins therefore have intrinsic folding characteristics that facilitate their rapid folding, and this is assisted by a variety of folding factors, including enzymes, peptides and metal ions. Despite these intrinsic and extrinsic factors, secretory proteins do misfold, particularly if the cell is subjected to certain types of stress. Consequently, Gram-positive bacteria such as Bacillus subtilis encode membrane- and cell wall-associated proteases that act as a quality control machine, clearing misfolded or otherwise aberrant proteins from the translocase and the cell wall.
- [Show abstract] [Hide abstract]
ABSTRACT: Vaccination against Chlamydia pneumoniae would be a beneficial strategy for either preventing or controlling infection by this human respiratory pathogen that also causes persistent infections. In the present study, we used recombinant Semliki Forest virus (rSFV) particles for delivering C. pneumoniae antigens major outer membrane protein (MOMP) or outer membrane protein 2 (Omp2) to the mice or applied the prime-boost technique, where mice were first primed with naked DNA and then boosted with the viral vector coding for the same proteins. Partial protection suggested by the reduced number of cultivable bacteria from the lungs of the challenged mice was seen in mice immunized by either method with MOMP expressing constructs. A significant protection was also achieved after DNA/rSFV immunization with Omp2. DNA priming followed by rSFV boosting induced a more prominent IFN-gamma production after challenge at the site of the infection in pulmonary and mediastinal cells.
- [Show abstract] [Hide abstract]
ABSTRACT: The transport of proteins from their site of synthesis in the cytoplasm to their functional location is an essential characteristic of all living cells. In Gram-positive bacteria the majority of proteins that are translocated across the cytoplasmic membrane are delivered to the membrane–cell wall interface in an essentially unfolded form. They must then be folded into their native configuration in an environment that is dominated by a high density of immobilised negative charge—in essence an ion exchange resin. It is essential to the viability of the cell that these proteins do not block the translocation machinery in the membrane, form illegitimate interactions with the cell wall or, through intermolecular interactions, form insoluble aggregates. Native Gram-positive proteins therefore have intrinsic folding characteristics that facilitate their rapid folding, and this is assisted by a variety of folding factors, including enzymes, peptides and metal ions. Despite these intrinsic and extrinsic factors, secretory proteins do misfold, particularly if the cell is subjected to certain types of stress. Consequently, Gram-positive bacteria such as Bacillus subtilis encode membrane- and cell wall-associated proteases that act as a quality control machine, clearing misfolded or otherwise aberrant proteins from the translocase and the cell wall.
Article: Structure-Function Analysis of PrsA Reveals Roles for the Parvulin-like and Flanking N- and C-terminal Domains in Protein Folding and Secretion in Bacillus subtilis[Show abstract] [Hide abstract]
ABSTRACT: The PrsA protein of Bacillus subtilis is an essential membrane-bound lipoprotein that is assumed to assist post-translocational folding of exported proteins and stabilize them in the compartment between the cytoplasmic membrane and cell wall. This folding activity is consistent with the homology of a segment of PrsA with parvulin-type peptidyl-prolyl cis/trans isomerases (PPIase). In this study, molecular modeling showed that the parvulin-like region can adopt a parvulin-type fold with structurally conserved active site residues. PrsA exhibits PPIase activity in a manner dependent on the parvulin-like domain. We constructed deletion, peptide insertion, and amino acid substitution mutations and demonstrated that the parvulin-like domain as well as flanking N- and C-terminal domains are essential for in vivo PrsA function in protein secretion and growth. Surprisingly, none of the predicted active site residues of the parvulin-like domain was essential for growth and protein secretion, although several active site mutations reduced or abolished the PPIase activity or the ability of PrsA to catalyze proline-limited protein folding in vitro. Our results indicate that PrsA is a PPIase, but the essential role in vivo seems to depend on some non-PPIase activity of both the parvulin-like and flanking domains.
Article: Probing the ability of the coat and vertex protein of the membrane-containing bacteriophage PRD1 to display meningococcal epitope[Show abstract] [Hide abstract]
ABSTRACT: Bacteriophage PRD1 is an icosahedral dsDNA virus with a diameter of 740 A and an outer protein shell composed of 720 copies of major coat protein P3. Spike complexes at the vertices are composed of a pentameric base (protein P31) and a spike structure (proteins P5 and P2) where the N-terminal region of the trimeric P5 is associated with the base and the C-terminal region of P5 is associated with receptor-binding protein P2. The functionality of proteins P3 and P5 was investigated using insertions and deletions. It was observed that P3 did not tolerate changes whereas P5 tolerated changes much more freely. These properties support the hypothesis that viruses have core structures and functions, which remain stable over time, as well as other elements, responsible for host interactions, which are evolutionally more fluid. The insertional probe used was the apex of exposed loop 4 of group B meningococcal outer membrane protein PorA, a medically important subunit vaccine candidate. It was demonstrated that the epitope could be displayed on the virus surface as part of spike protein P5.
Article: Production of Chlamydia pneumoniae Proteins in Bacillus subtilis and Their Use in Characterizing Immune Responses in the Experimental Infection Model[Show abstract] [Hide abstract]
ABSTRACT: Due to intracellular growth requirements, large-scale cultures of chlamydiae and purification of its proteins are difficult and laborious. To overcome these problems we produced chlamydial proteins in a heterologous host, Bacillus subtilis, a gram-positive nonpathogenic bacterium. The genes of Chlamydia pneumoniae major outer membrane protein (MOMP), the cysteine-rich outer membrane protein (Omp2), and the heat shock protein (Hsp60) were amplified by PCR, and the PCR products were cloned into expression vectors containing a promoter, a ribosome binding site, and a truncated signal sequence of the α-amylase gene from Bacillus amyloliquefaciens. C. pneumoniae genes were readily expressed in B. subtilis under the control of the α-amylase promoter. The recombinant proteins MOMP and Hsp60 were purified from the bacterial lysate with the aid of the carboxy-terminal histidine hexamer tag by affinity chromatography. The Omp2 was separated as an insoluble fraction after 8 M urea treatment. The purified proteins were successfully used as immunogens and as antigens in serological assays and in a lymphoproliferation test. The Omp2 and Hsp60 antigens were readily recognized by the antibodies appearing after pulmonary infection following intranasal inoculation of C. pneumoniae in mice. Also, splenocytes collected from mice immunized with MOMP or Hsp60 proteins proliferated in response to in vitro stimulation with the corresponding proteins.
Top Journals
National Institute for Health and Welfare, FinlandHelsinki, Southern Finland Province, Finland
National Public Health InstituteHelsinki, Southern Finland Province, Finland
Institut Jacques MonodLutetia Parisorum, Île-de-France, France
University of Helsinki
Helsinki, Uusimaa, Finland
- Recombinant DNA Laboratory
European Molecular Biology LaboratoryHeidelburg, Baden-Württemberg, Germany
University of California, BerkeleyBerkeley, California, United States
Statens kriminaltekniska laboratoriumLinköping, Östergötland, Sweden


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APP OF THE DAY - Taptu (Android)
Do you ever find yourself swamped with information? Too many sites and not enough time to read them all? Do you want to snack on the content of your favourite websites without having to actually visit them?
If this sounds like you, you're probably reading this via the RSS feed. If that's your thing, have you ever thought about repackaging all that content and reading it all via an app? Fear not, as today we're looking at just the thing.
- Format
- Android
- Price
- Free
- Where
- Android Market
Taptu is available on a number of platforms, but given the current love-in for Honeycomb tablets, we've been taking it for a spin on the Motorola Xoom. Taptu is optimised for Honeycomb and given it's status as an aggregator of content, it's well suited to the larger screen, and you'll find controls in the action bar as you would with other native Honeycomb apps.
We'll be honest, it isn't that different from Pulse that we looked at last month, which also collects and offers you up content from your favourite sites. If you're using Pulse, you'll probably be disinclined to swap to Taptu, but it does offers some nice features.
Content is arrayed on scrolling banners, picking up images and titles from your selected sources and giving you the option of clicking through to read more. You'll get as much content as the source site puts out in its feed, so the results are dependant on what you pick.
Taptu offer up a range of featured and categorised sources for you to pick from, with a link to Google Reader (although when we tested the app this resulted in an error). Once setup, you can rearrange content to your liking, including merging streams.
You could dump all your tech websites into one bucket of gadgety filth, or all your sport fan sites in to one pot. There are some curated feeds already and you can break those apart to get the individual sites if you want to.
You can also search easily for your favourite site to see if a feed can be found. If Taptu can't find it, you can ask Bing to have a look - often you'll get a positive result.
Adding a little more interest, you can also consume your own social news by linking up with Facebook or Twitter, as well as getting sharing options back to those networks - as well as any other apps you've got installed as it normal with Android - so it's easy to tell people what you've found interesting, or come back to it later.
Some sites work much better than others and we found some content provided simply offered up the advertising page that sits between a site's feed and the actual page. It's irritating because you then have to view it in the browser instead, or just dump the offending sites.
You also get a widget and you can create and customise as many widgets as you want, but we found it rather lacklustre and doesn't yet take advantage of Android 3.1 widget resizing, which could make it much more useful.
Overall it's a great little app with plenty of versatility that works nicely on Honeycomb and well worth checking out if you like your content from lots of different places.
Happy reading.
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Oh how the months speed by…
The times folks have paused along Lake Superior Spirit's shores since 2010
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The latest from Our Little House in the Big Woods
- Until we meet again, my friends…
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- Battened down, eating sweet squash pie & not blogging much
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Daily Archives: August 25, 2010
The magic of eagle feathers, amphibians and autumn leaves between Point A and Point B
The computer monitor of my desktop "work computer" has been slowly dying. It started before our Duluth trip. Suddenly the words and pictures looked smeary, unreadable. Almost like the viewer badly needed a new pair of glasses. Yesterday–when I noticed I … Continue reading
Posted in Uncategorized Tagged autumn, Baraga County, beach, computer, gratitude, Keweenaw Bay, Lake Superior, life, musings, nature, outdoors, peace, personal, philosophy, photography, spirituality, summer, technology, thoughts, travel, Upper Peninsula 29 Comments


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Originally posted by Praetorius
reply to post by SG-17
That's a novel idea. I hadn't heard about the DNA before, but I always thought they'd be more along the lines of gigantopithecus, and the lack of corpses probably due to a combination of their habitat, possible instinct of moving to remote or hidden 'graveyards' (like elephant graveyards?), and/or how quickly remains will break down in temperate climates.
Would only a single base-pair difference allow for such a different appearance, etc.?
Originally posted by SG-17
What if Bigfoot is an early human species, not quite homo sapien, but also not homo erectus, homo heidelbergensis, or homo neanderthalis? It could have crossed the Bering Land Bridge long before modern humans did, and if it is an early human species it would explain why they are so elusive.
Just look at gorillas, they are excellent hiders. It took hundreds of days of dedicated searching to find the first gorilla tribe. Imagine that but with higher intelligence. If Bigfoot is a member of the homo genus it should be smart enough to identify areas of likely human activity and avoid them during peak hours and only use them when humans are not likely to be there. They would also be likely to bury their dead, or place their dead in special locations like caves. This would explain why we haven't found a carcass. It would also explain why the DNA acquired from the screw board at the fishing cabin at Snelgrove Lake was human with the exception of a single base-pair, compared to a 35 base-pair difference in great apes.


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2008 Honors Faculty and Student Survey Report
The 2008 Honors Faculty and Student Surveys were constructed to assess the awareness and satisfaction of the Honors Program. The evaluation of the program included Honors faculty, Honors students and non-Honors students. The data was utilized to inform Honors Program Review.
Non-Honors Students Frequency Distributions
Alumni Frequency Distributions
Faculty Frequency Distributions


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It allows new & retrofit pipe work to be completed without draining the system. This kit will fit 1/4" through 2" diameter steel, copper, cast iron, plastic or aluminum pipe. For a single or double freeze.
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- VietnamFoodFunding via Thanh Hoa Microfinance Institution Limited Liability
White Sand ...VietnamFoodFunding via Thanh Hoa Microfinance Institution Limited Liability
All For Chi...VietnamFoodFunding via Thanh Hoa Microfinance Institution Limited Liability
040121 Giang Sơn - Hoằng Trường Group
She is the leader of a 4-member group, the 040121 Giang Sơn - Hoằng Trường solidarity group. Hạnh supports 3 dependents with her business in the Hoang Hoa district, a rural town in Thanh Hoa province. In 2010, Hạnh joined the Thanh Hoa Fund for Poor Women to improve her business. Hạnh has successfully repaid 3 loans from this fund. Her main hardship is the lack of capital. In the future, Hạnh hopes to maintain a successful business and to have her children do well in school.
Additional Information
About Thanh Hoa Microfinance Institution Limited Liability (Thanh Hoa MFI)Thanh Hoa MFI works to provide credit to women and their families in the central Vietnamese province of Thanh Hoa, one of the poorest regions of the country. The institution's target clients are poor and low-income women who have the ability to work but lack the capital. Accordingly, Thanh Hoa MFI prioritizes single mothers, low-income women with children who have dropped out of primary school, and Viet Nam's poorest women who cannot access any formal financial services.
This is a Group Loan
In a group loan, each member of the group receives an individual loan but is part of a larger group of individuals. The group is there to provide support to the members and to provide a system of peer pressure, but groups may or may not be formally bound by a group guarantee. In cases where there is a group guarantee, members of the group are responsible for paying back the loans of their fellow group members in the case of delinquency or default.
Kiva's Field Partners typically feature one borrower from a group. The loan description, sector, and other attributes for a group loan profile are determined by the featured borrower's loan. The other members of the group are not required to use their loans for the same purpose.
About Vietnam
136View loans »
Success!! The loan was 100% repaid
- Repayment Term
- 14 months (Additional Information)
- Repayment Schedule
- Monthly
- Pre-Disbursed:
- Feb 22, 2013
- Listed
- Mar 2, 2013
- Currency Exchange Loss:
- Possible
- Ended:
- Mar 17, 2014


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Modest Medieval scholar's commentaries on Aristotle come to light after five hundred years
Tracking down a watershed moment for labor.
Manhattan harbors hotel gem from the 1820s.
Site on medieval history, broad in scope, ranges from the totally wacky to materials that are deep and philosophically chellenging.
Memphis preacher's message could be found in his photographs.
In Baltimore, the museum of industry charms.
Woodrow Wilson's Fourteen Points: Raising expectations of emerging national leaders.
Adolf Cluss lived through revolutionary times, first in his native Heilbronn in southwest Germany, where as a young man he got swept up in the popular uprisings of 1848, and then in Washington, D.C.,
A little rusty on the Dante you read as an undergraduate? Like to brush up a bit on some points in the Inferno, like that "mal" something?
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Humboldt in the New World
Journeying through South America, Alexander von Humboldt sought nothing less than "the unity of nature."
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Done with Tolstoy
Famed translators Pevear and Volokhonsky reach another milestone.
By Kevin Mahnken
A Workingman's Poet
Frankness and plain speaking made Carl Sandburg a celebrity.
By Danny Heitman
The Blue Humanities
In studying the sea, we are returning to our beginnings.
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Ralph Waldo Emerson
What accounts for Emerson's endurance as a writer?
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On May 19, 20, and 21, 2015, many of the foremost researchers in occupational injury convened in Kingwood, West Virginia at the Camp Dawson Training Center for the sixth National Occupational Injury Research Symposium (NOIRS 2015). The National Institute for Occupational Safety and Health (NIOSH), in partnership with the American Society of Safety Engineers (ASSE), the Liberty Mutual Research Institute for Safety (LMRIS), the National Safety Council (NSC), and the Society for Advancement of Violence and Injury Research (SAVIR), hosted NOIRS 2015 to provide a forum for researchers and others to share their findings and methods related to traumatic occupational injury.
NOIRS 2015 was the sixth such symposium focusing on research for the prevention of work-related traumatic injuries. The theme was "Advancing Occupational Injury Research through Integration and Partnership." The agenda included contributed oral presentations in concurrent sessions and poster presentations which described research studies, methods, and findings related to traumatic occupational injuries.
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Adopted a resolution calling upon the Legislature to pass NO law changing the status of local board of education elections. Motion carried 7-0. President Club Member Chloe Cudney gave a snap shot of activities that are happening in the classrooms at Marysville High School. Reviewed a budget report, cash balance report, and budget projections. Discussed technology infrastructure and bids from …Read More »
Tag Archives: board of education
Marysville USD 364 Board of Education Meeting Highlights
Marysville School Board Renews All Head Coaching Contracts
The Marysville School District Board of Education met on Thursday. The board confirmed extra duty assignments for 2011-2012 School Year coaching and extracurricular assignments. The contracts with all of the 2010-2011 head coaches were renewed by the board.Read More »
USD 115 Hires Certified and Office Staff
The Nemaha Central USD 115 temporary board of education has extended contract offers to certified staff for the 2011-2012 school year. Superintendent Darrel Kohlman said the action followed a closed door session during a special meeting Monday evening. The board also approved the terms and conditions of employment for the teaching staff and hiring of the central office staff.Read More »
USD 115 Searching for a New Name
The USD 115 temporary board of education met Monday. They approved having each building administrator in Nemaha Valley USD 442 and B & B USD 451 chose a person to serve on the committee to select the new name for USD 115. Superintendent Darrel Kohlman said the committee will present their recommendations during the board's next meeting on January 17th.Read More »


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Posts Tagged 'bookstores'
Tales from the Void
April 17, 2015 | by Dan Piepenbring
The art collective Le Gun (Steph von Reiswitz, Neal Fox, Chris Bianchi, and Robert Greene) has mounted "Tales from the Void," a stealthy takeover of the Shakespeare and Company bookstore, that Parisian mainstay. Their installation comprises hand-drawn "sculptural books"—many with fake but disarmingly plausible titles like Encyclopedia of Beatnikism and The Minotaur of Montmartre—hidden among the shop's shelves. You'll find some favorites below.
They've also completed the large-scale drawing above, a sprawling tribute to the history and culture of the bookshop that depicts various writer personages, including—count them all—George Whitman, Michael Smith, Ezra Pound, Gregory Corso, Olympia, Lawrence Ferlinghetti, James Joyce, Paul Auster, Frank Sinatra, Colette the Dog, Martin Amis, Henry Miller, Aaron Budnik, Richard Wright, Sylvia Whitman, Lawrence Durrell, Allen Ginsberg, Jack Kerouac, Ernest Hemingway, F. Scott Fitzgerald, Gertrude Stein, Alice B. Toklas, James Baldwin, Sylvia Beach, Ray Bradbury, William Burroughs, Dionysus, James Jones, Zadie Smith, the "generic spirit of Beatnikism," Anaïs Nin, and Kitty, the shop's resident cat. Read More »
$190,000 Birds, and Other News
February 6, 2015 | by Dan Piepenbring
- Latin, the most famous dead language, is enjoying another of its many posthumous lives: "A language can fall out of everyday use, its forms can cease to change, and yet writers will still use it to do new things. This happened to Sumerian and Hebrew—and it happened to Latin too. People all over the Mediterranean world and beyond continued to use Latin after Virgil and Cicero—and they did so in endlessly creative ways."
- The hazards of open endings: Why does so much literary fiction refuse to provide a real resolution? "An authorial strategy now so widespread to have almost become the norm in literary fiction was so 'unfamiliar' back in 1925 that Woolf suggested readers 'need a very daring and alert sense of literature to make us hear the tune.' "
- A 1765 book about ornithology has sold for $190,000: "Published in Florence in Italian in five volumes, it contains 600 beautiful hand-colored engraved plates of birds. Commissioned by Maria Luisa, the Grand Duchess of Tuscany, the book took ten years to complete … Some consider the book to be a commentary on 18th-century Italian high society because the bird poses are almost human."
- Technicolor turns 100: "We realize that color is violent and for that reason we restrained it," an early adopter once said. But today, Technicolor has developed "this very vibrant, saturated palette ... When these films started getting more colorful, that's what audiences reacted to. They loved this artificial, fantasy, over-the-top palette. And that's the way color shifted. It's idealized."
- Running a bookstore is hard. Running an anarchist bookstore is even harder. And not because of the anarchy, it turns out—because of the antianarchy. At San Francisco's Bound Together, "there've been plenty of adventures, like the time when the bookstore was threatened by Neo-Nazis in the eighties and members slept in the space nightly to protect it. There was also an attempted arson in the eighties, when someone dumped gasoline through the mail slot and tossed a lit match in to start a fire."
Good News
January 28, 2015 | by Sadie Stein
Today, fans of the Bonnie Slotnick Cookbooks store received a welcome e-mail. "Bonnie Slotnick Cookbooks—MOVED!!" read the triumphant subject line. After being forced to leave its longtime home on West Tenth Street, and facing an uncertain future, the beloved institution has landed safely in a new location in the East Village. Many who love the terrific antiquarian shop—stocked with centuries' worth of culinary history, lore, and recipes—and its knowledgeable owner have breathed a sigh of relief.
Especially in cities, we're all so used to seeing independent businesses and brick-and-mortar bookstores die—we're almost inured to it—that it feels strange to get good news. Usually, we give our heads a mournful shake and think, Well, it was too good to last. But, thanks to the generosity of a pair of siblings who are providing a great space at an affordable rent, the shop will not merely survive, but enjoy three times its old space, plus a garden. As Bonnie wrote in an earlier e-mail, "What Margo and Garth [the aforementioned siblings] have done is extraordinary in this day and age, and especially in this city."
The 28 East Second Street location promises to be up and running by early February; do go by if you can. As Bonnie writes, "I'm looking FORWARD. CHANGE IS GOOD! Repeat after me: CHANGE IS GOOD!" (Well, occasionally, anyway.)
Sadie Stein is contributing editor of The Paris Review, and the Daily's correspondent.
Inside Albertine
September 26, 2014 | by Dan Piepenbring
Nations of the world, take note: there are a number of benefits to running an embassy out of an historic mansion on Fifth Avenue. First, look around: you're in an historic mansion on Fifth Avenue! Second, go upstairs: you're still in that same historic mansion, on the same Fifth Avenue! Third, take stock of the fact that, because you don't pay rent, you can kiss off market forces and open any business you'd like … in your historic mansion on Fifth Avenue!
Antonin Baudry, the cultural counselor for the French Embassy, had such a realization a few years ago. For more than sixty years, the embassy has made use of the Payne Whitney House, an opulent Italian Renaissance–style home erected from 1902 to 1906 at Fifth Ave. and 79th St. It seemed a shame, he thought, to deny passersby the chance to see its tongue-lollingly gorgeous interior. It also seemed a shame that New York had lost its last French bookstore, the Librairie de France, in 2009 …
You may see where this is headed. Baudry and his staff are at this moment putting the finishing touches on Albertine, a new French bookstore housed in the embassy—it opens Saturday at eleven A.M. When I visited yesterday, Baudry showed me around its impressive two floors, which had already achieved—though the ladders and drop clothes were still in evidence, and the painters were still painting, the burnishers still burnishing—an enviable blend of new bookstore smell and old building smell. It resembles a magnificent private library of the sort you'd expect to find in a turn-of-the-century estate. Read More »
Crazy Heroines Should Stay That Way, and Other News
June 6, 2014 | by Dan Piepenbring
- It's the fiftieth anniversary of Joanne Greenberg's I Never Promised You a Rose Garden, a YA novel about her time as a schizophrenic teen. "Not long after I Never Promised You a Rose Garden became canonical, it also became a lightning rod … Greenberg claimed full recovery, and many psychiatric professionals worried that this would inspire a false and dangerous hope. Schizophrenics, they said, simply cannot recover."
- A new location (and ambiance) for Foyles, "London's temple of books": "Light streams down from rooftop windows on to a spacious white-walled atrium catching the edge of a 1930s dancefloor like a spotlight. It's a huge change of scene for Foyles … which was once famed for dark forbidding bookshelves and a payment system so arcane that many visitors chose to steal rather than buy their favored paperback."
- Chester Nez, the last of the original Navajo code-talkers, has died at ninety-three.
- What if it were your job to deliver bad news all day? You would succumb to a deep and enduring existential malaise, experts say.
- "The best way to think about the universe is as a multidimensional space-time loaf."
That's Van Gogh's Ear, and Other News
June 5, 2014 | by Dan Piepenbring
- Now on display at a German museum: a replica of one of Van Gogh's ears. (Hint: it's not the one he didn't cut off.) "Created using 3D printers and genetic material from a living relative of van Gogh, it was shaped to be the exact size of the Dutch painter's ear and is kept alive in a nourishing liquid."
- Yesterday's usage wars were every bit as fraught and irrational as today's: "'Dilapidated' was frowned upon by some because it comes from a Latin root, lapis, meaning stone, so it was thought that you should only refer to a dilapidated building if it was actually made out of stone … And it was considered that luncheon was the proper noun and that lunch was really only to be used as a verb."
- What chemical compounds produce the smells of new and old books? Vinyl acetate ethylene, alkyl ketene dimer, and 2-ethyl hexanol, of course!
- Tales from New York's bookstores: "One day a woman asked us which Jennifer Egan book she should read … We recommended Look at Me, and then suggested, 'If you'd like it signed, Jennifer Egan is right next to you and is quite nice.'"
- Centralia, Pennsylvania: still on fire. Has been since at least '62.


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Want a different start date or number of nights? View other dates available.
based on73 reviews
Superb spacious and contemporary beautifully furnished and well equipped lodge. One double (some with four poster bed) and one twin. Bath and separate shower cubicle. Outdoor hot tub. Fri and Sat starts.
Please call 0345 498 6060 (or +440345 498 6060 if calling from outside the UK) as we need to check this booking for you.
Superb spacious and contemporary beautifully furnished and well equipped lodge. One double (some with four poster bed) and one twin. Bath and separate shower cubicle. Outdoor hot tub. Fri and Sat starts.
Please select an available date from the calendar to proceed with your booking.
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out of 10
We stayed in the "hedgehog" retreat lodge which was private. some of the other lodges do have footpaths leading next to them and overlooking the hot tub although the owners do state that on the site. If you want a private lodge ask for the "Hedgehog" lodge.
Confirmed guest, 2015-10-23
The lodge was the highest standard I have stayed in and had everything and more to make your holiday perfect. Again all the little touches made it special. I can find. I fault with anything.
Confirmed guest, 2015-10-17
out of 10
lodge had everything you needed!
Confirmed guest, 2015-09-28
Fantastic !! Very clean! Comfortable
Confirmed guest, 2015-09-25
out of 10
Lodge was lovely and clean welcome pack was lovely. Hot water was abit temperamental. Bed was comfy covers where clean but had holes in them.
Not enough towels supplied, we brought some from home which was a good job. The light fitting in the bedroom was hanging down. There was a lot of dust up the walls it had stuck to the wood.
Confirmed guest, 2015-09-19
Our lodge was beautiful, really well equipped and a lovely place to spend our family holiday. It was also baby friendly, which made the holiday with our 18 month old toddler more relaxing!
Confirmed guest, 2015-09-12
out of 10
It was nice but there were lots of cobwebs and dead splattered spiders inside the lodge which could have been cleaned up and the main bedroom smelt damp but the lodge was still ok
Confirmed guest, 2015-09-05
Confirmed guest, 2015-08-29
Complimentary tray on arrival was a beautiful touch.
Lodge had everything you needed.
Confirmed guest, 2015-08-15
Lovely lodge, well stocked. Modern kitchen & bathroom. Excellent hot tub
The best lodges we have stayed in.
Confirmed guest, 2015-07-25
out of 10
Bed in main room is really hard and pillows overly soft a TV in the room would be a nice touch also no hoover
Confirmed guest, 2015-07-18
Clean, comfortable, well maintained. In the wet weather our lodge had a bit of a dmap feeling to it, but that was due to it's location on the site (shaded) and the poor weather. We were able to turn the heating on which was fine.
Hot tub is great
Confirmed guest, 2015-07-13
Again as above
Confirmed guest, 2015-07-10
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Superb beautifully furnished and well equipped lodge. One double with ensuite shower, one twin and one with bunks (suitable for children under 14). Bath and separate shower cubicle. Outdoor hot tub. Sat start.
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Modern and contemporary lodge. One double with flat screen TV/DVD and one twin. Bath and separate shower cubicle. Outdoor hot tub. Fri start.
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text: Gi - Halo 5 fans wondering what The Arbiter has been up to since signing a peace treaty with Admiral Hood and returning to Sanghelios (the Elite homeworld) need look no further than the Halo novels. Imagine that after thousands of years of training an alien race for military positions, the system that required those positions collapses leaving the majority of those aliens jobless. Then imagine that as a result of that collapse, most of that race's labor force, comprised of other alien races, dries up leaving no training manuals behind. So far not good right? Now imagine that these same aliens have been hard-core religious for thousands of years and they just found out their religion is bull. This sums up what has been happening with the Elites since the end of the Covenant war with the humans.


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Home > Restaurants >
773 Tenth Ave., New York, NY, 10019
at 52nd St.
By Damejayne on 4/2/2004
My friend from Ramallah feels that this is just like food his Mom used to make...only better. Order a bunch of the appetizers each one more delicious than the next and made for sharing. Only order the shrimp entree if there are three or more people at the table. Its been my experience that the person who gets that dish also stops talking till they're finished. Food here is so natural - each mouthful seems like the first with a fresh explosion of flavors. The deserts are simply unbelievable.
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In 1997, Robert's book Rich Dad, Poor Dad stunned listeners, stating, "Your house is not an asset." As howls of protest went up around the world, the book went on to become an international best seller, one of the longest-running best sellers in New York Times history.
Rich Dad, Poor Dad is not a book on real estate. It is a book about the importance of financial education. Rich Dad, Poor Dad was written to prepare you and your loved ones for the financial turbulence Robert's rich dad saw coming.
In 2007, as homes declined in value or were lost to foreclosure, millions of homeowners painfully discovered the wisdom in Robert's rich dad's words.
Today, we are all aware that a home can be a liability. Today, we all know a home can go up or down in value. Today, we all know a person can lose money investing in the stock market. Today, we all know our money can go down in value and even savers can be losers.
This is why your financial intelligence is more important today than ever before. In a world of financial turbulence, your best asset is your financial IQ.
"This book is about choosing new options, new directions, and a new financial future." (Robert Kiyosaki, author)
©2013 Robert T. Kiyosaki (P)2013 Brilliance Audio, Inc.
"Not as Good as Rich Dad Poor Dad"
Although an informative book this is mostly for people who are interested in starting their own businesses. Make sure you listen to his books in order as this one does make references to previous books.
"Should be required listening"
This is the type off book that is probably better in written form but only because of the need to stop regularly to write notes and examine diagrams. There is so much useful information in this book that I found myself having to stop the program to give myself reminders and write down ideas - a little bit annoying with the audio, but at least one can pick up right where one left off.
It is filled with "aha!" moments - times when something that Kiyosaki says just makes sense of something in the world. I lost count of the number of these as I listened to this book on a long drive.
Tim was clear and easy to understand and didn't become boring - even after nine hours of listening.
Become smarter about money.
This book should be required listening.
I listen to a lot of Audio Books while working :)
"Everyone should read this"
I can't stress enough how crucial this book will be in my future. This, and all of the other Rich Dad books I have read, have altered my life path permanently and for the better. Listen to this audiobook, it can only help.
"Good concepts."
The book has good basic information. The concepts are easy to understand, but they lack direct practical implementation. Would have been nice for him to provide more examples of how get got started making his millions.
"First half is good"
First half sparks that fire in you and gets your mind thinking. Second half feels redundant to the other books.
Excellent overall book, great advice, great stories and easy to follow. Sad when it ended. Recommend to everyone to read
Excellent book a must read for people looking to learn how currency works and separate themselves from the norm.
"Really Let Down"
Loved his first book, so I had high expectations here. However, this was nothing more than a few stories about things he had done, with no real points or advice. Certainly not on par with the original Rich Dad, Poor Dad.
"2nd time going through this book"
I knew I had to review this book, especially going into this year's presidential elections. Would highly recommend this book!
"Narration was great"
This book is good and simple. I wish it had less agenda in the back ground. Some times he teaches you how and other times he just rambles.
"Buy it now"
This is essential for anyone who wants a better life. Listen and start becoming an investor and stop buying liabilities. Really good and buy it now!
"Bull's eye"
Finished it in one day. Will start all over again! and again. Then in the future.
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Re: Starting out my credit rebuilding project Need advice
Shogun wrote:
I see those numbers are going up. Great job!
Thanks!! I cant wait till my OpenSkyy CC reports $0 balance in a few days (Currently at $289/$300 limit) as well as my CU $500 secured and applied bank $200 secured!!
Hopefully i can get close to 600 with those
Starting Score: Exp:513 | TU:541 | EQ:517 - 11/13/2012
Current Score: Exp:623 | TU FICO:639 | EQ FICO:743 - 01/24/2012
Wallet: Applied Bank $200, OpenSkyCC $300, 1st Tech CU Secured $2k, Discover IT $1.5k, CITI AA $2.8k, USAA AMEX $7.5k
Take the FICO Fitness Challenge
(1,299 Views)


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National Urban League President Marc Morial
"Every Father's Day, I honor the memory of my father, Ernest 'Dutch' Morial, and the incredible example that he set for my siblings, me and many others," said NUL president and CEO Marc Morial, pictured left, with his parents and brother. "So many Black men raise their biological children and serve as father figures to countless more. We understand that our children don't just need our pocketbooks, they need our presence. Beyond our discipline, they need our direction. As important as the lessons that we teach them is the love that we give them. My father taught me these things through example, and I hope that one day my three children will say the same."
(Photo: Courtesy of Marc Morial)
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Black America Is in the Red: A Breakdown of the Stats04/16/2015
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Rocker Ozzy Osbourne has applauded British royal Prince Harry for injuring a photographer's lip last week (21OCT04).
The BLACK SABBATH star hopes the fracas outside a London nightclub will teach the paparazzi to respect the monarchy.
He says, "I couldn't agree more with what he's done. That boy and that family get such a hard time and he's been pushed too far and lashed out.
"The other bloke absolutely had it f**king coming to him. I think it's b**ody great what Harry did. It's all about getting respect and Harry wasn't getting it.
"In the past if a royal was caught up in a situation like that he'd say, 'Oh George, someone's f**king bothering me.' Not Harry, he just turned around and lamped the b**tard."27/10/2004 09:19


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Top Definition
Having a disagreement with your significant other about shopping at Target too much.
After shopping at Target for 3 days in a row, she suggested going again, that's when we got into a Targuement.
by Rodney Riddle February 17, 2012
Type your email address below to get our free Urban Word of the Day every morning!
Emails are sent from email@example.com. We'll never spam you.×


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The fallen tree at Aldersbrook Primary School has led to concern over security
Douglas Patient, Reporter, covering Waltham Forest. Call me on 07824 530 127 / / News
A headteacher has expressed concern over security at her school after a 20ft tree smashed through new fencing.
The tree at Aldersbrook Primary School, Northumberland Avenue, Wanstead, was blown over during storms on Thursday night.
The top of the tree was cut off by the council to unblock the road, but is caused significant damage to the fence and there are fears other trees could fall.
Aldersbrook Primary School headteacher, Miz Mann, said: "I have come into school to find a tree at the back of the large playground has fallen through the new fencing and into the road.
"Fortunately this happened with no children or staff on site.
"My concerns are the security of the site and that the trees were all assessed just before summer 2013 by the council before the works on the fence were due to commence.
"I appreciate the weather has been adverse. However, my concern is now that we need to ensure all our trees on site are not going to cause any further safety risks."
A council spokesman said: "We have a rolling programme of tree checks for Redbridge, but the trees in and around the school will be looked at sooner if they are believed to be dangerous."
Promoted Stories
Concern over safety and security of children after tree in Wanstead felled by high winds.
Is the height of it relevant? I hope they replace those original features. They have ruined many schools around Walthamstow and Leyton with modernisation.
Is the height of it relevant? I hope they replace those original features. They have ruined many schools around Walthamstow and Leyton with modernisation.


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How to Wear Chiffon in the Daytime
Hi Imogen, I would really love you to cover how to wear chiffon as day wear. Being a large busted H shape means that…Read More
Hi Imogen, I would really love you to cover how to wear chiffon as day wear. Being a large busted H shape means that…Read More
Frequently I'm asked "As an image consultant do you always have to dress up?". My answer is, "Well I still wear jeans and t-shirts,…Read More
There are a plethora of style rules to follow and sure they'll help you look fabulous and flatter you body and colouring. But doesn't…Read More
.Here is the lovely Jan Fisher, Image Coach again, we bought this dress (same shop as we bought the beads) on Monday. It's a…Read More
I was lucky enough to spend the day yesterday with Jan Fisher, AICI FLC a New Zealand based image consultant who you have met…Read More
.What are the features that an H Shape should avoid taking to the change room to stop depression setting in when on a shopping…Read More
..Reader Kanerou asks: Do you have any plans to do an entry on dressing for plus-sized Xs? I have a couple pairs of black…Read More
Here again is my gorgeous friend Jan Fisher, Image Coach, from New Zealand – who is heading fast to 60! Red is one of…Read More
I've had quite a few requests from my lovely readers to do some posts on 50+ dressing, so rather than me do up another…Read More
If I had to describe my personal style in 3 words it would be creative, dramatic, feminine. When I discovered my personal style preferences…Read More


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Nile crocodile by AngiWallaceWatch
©2012-2016 AngiWallace
A Young Nile crocodile
This is a very impressive and realistic depiction of a crocodile. Thanks for sharing!
Beautiful work!
Wonderful detail. A fine portrait.
Incredible shot!
Beautiful work, this is amazing !!
Very nice! It has a sureal sort of primeval look to it.


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This content has been marked as final. Show 2 replies
Re: Activate GPS on ArcGIS Online?tesla007 Apr 27, 2012 2:35 PM (in response to amalkawi)Try using Arcgis Mobile, or the Android & iPhone ESRI ArcGIS App. You can also use ArcMap, however I've never tried it.
Re: Activate GPS on ArcGIS Online?jrflannery Apr 29, 2012 9:08 AM (in response to amalkawi)Anas:
Try using the ArcGIS Explorer Desktop application. Click on the Add Content button and choose ArcGIS Online. Add your online map. Click on the Display tab in AGX Desktop and then click on the Receive Signal check box in the GPS Receiver options. We have found success using AGX Desktop with a Microsoft Trips & Streets GPS receiver and Verizon cellular card on laptops in the field.


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So like,I shot this movie for a student/friend of mine over the summer. He's finally got it all put together and will be releasing it Oct 21. Here's the trailer:http://www.youtube.com/forrestfire101
and ya get to see what I look like as a zombie bounty hunter.
hello,am a musician also a film editor i need a dslr camera with the best video quality specially for music video within this price rage $2000.
I shot with a DSLR and I have been looking into getting an xlr adapter box such as the one made by Beachtek so I can use xlr mics. After thinking, I was wonder what the difference would be if I just bought an xlr to 1/8 (1/8 goes into my dslr) cable as long as my mic is powered? Thats $10 vs $300...am I missing something here?
Some friends and I decided we'd like to try and do a short film. So we made an outline to write, score, film, and edit in 12 hours and this is the outcome! Shot entirely with a hacked GH1 and a 50mm canon fd lens
Any feedback is greatly appreciated.
Schizophrenic short film about a girl staying alone in the university dorm at a weekend. She starts to experience some inexplicable and mysterious phenomenons like time travels. There is also rumors about a guy who fell down the dorm stairs and died a few years ago.http://www.youtube.com/watch?v=MMGRPYA7cTkThis is my first DSLR short film and I'm new to this stuff. If you have any advice let me know, I'm open to constructive criticism.
Software:Adobe Premiere Pro CS3Adobe After Effects CS3
I hope you all don't mind viewing my blog to check out relevant film/videomaking information with me on this blog. It will help me as well as you(Learning never stops and I need to learn from the gurus here). I got some websites that may be of use to us all=]
Hello gentlemen and ladies...
I'm currently writing an article on capturing fluid on-screen movements as seen in action sequences and i'm pouring my brain juices out. Is there any feedback for how it can be done effectively using minimum equipment?
The article i wrote can be found here: http://fadlywychowvski.blogspot.com/2011/08/recording-fluid-action-movement-in-6.html
Perhaps I can include more tips and tricks into the article to edit it further. I did some research, but some is never enough. Gurus out there! help enlighten me please
Anyone of you guys watched the second webisode yet?http://www.zacuto.com/the-great-camera-shootout-2011/episode-two
I wrote an article about the shortcomings of DSLR filmmaking after watching Zacuto's 2011 Great Camera Shootout. Do check it out at http://fadlywychowvski.blogspot.com/2011/08/2011-zacutos-great-camera-shootout-part.html
and leave me comments and replies here=] Would be greatly appreciated to know what you guys think.
Hi Guys
I have a Sony Z1(E) but it's not something you have by your side all the time to shoot any unexpected/ interesting opportunities on the hoof. I've been looking for a camcorder that suits my budget to support my Z1 so I can take it on trips/ have in the car, etc.
Hey Everyone!!!
So sorry, if this is already posted somewhere or is under the wrong heading...
Okay so I'm an actor and photographer, but I am directing a music video for the first time. It's a group of 4 girls, one of which is a close friend of mine (kind of a pop feeling song). I don't doubt myabilitiesto execute it well, but some "experience" questions have come up in my mind and the answers are only in experience which I don't have so here I am...
Shoot Details:
Final Cut Pro X: File Management & Organization
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