text:
Editor: Craig Shoemaker
ISPsa2, the first mobile genetic element to be described and characterized in the bacterial facultative intracellular pathogen Piscirickettsia salmonis
Article first published online: 12 NOV 2010
DOI: 10.1111/j.1574-6968.2010.02132.x
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 314, Issue 1, pages 18–24, January 2011
Additional Information
How to Cite
Marshall, S. H., Henríquez, V., Gómez, F. A. and Cárdenas, C. (2011), ISPsa2, the first mobile genetic element to be described and characterized in the bacterial facultative intracellular pathogen Piscirickettsia salmonis. FEMS Microbiology Letters, 314: 18–24. doi: 10.1111/j.1574-6968.2010.02132.x
Publication History
- Issue published online: 3 DEC 2010
- Article first published online: 12 NOV 2010
- Accepted manuscript online: 13 OCT 2010 08:47AM EST
- Received 28 June 2010; revised 22 September 2010; accepted 27 September 2010.Final version published online 12 November 2010.
- Abstract
- Article
- References
- Cited By
- Piscirickettsia salmonis;
- insertion sequence;
- inverted repeats;
- transposase
Piscirickettsia salmonis is a novel, aggressive, facultative Gram-negative bacterium that drastically affects salmon production at different latitudes, with particular impact in southern Chile. Initially, P. salmonis was described as a Rickettsia-like, obligate, intracellular Alphaproteobacteria, but it was reclassified recently as a facultative intracellular Gammaproteobacteria. This designation has prompted the independent growth of the bacterium to a pure state for detailed study of its biology, genetics and epidemiology, properties that are still relatively poorly characterized. The preliminary sequence analysis of a 992-bp fragment of pure P. salmonis DNA allowed us to characterize a novel and complete 863-bp insertion sequence in the bacterial genome (named ISPsa2), which has a novel 16/16 bp perfectly inverted terminal repeat flanking a 726-bp ORF that encodes a putative transposase (Tnp-Psa). The coding sequence of the enzyme shares similarities to that described in some Bacillus species and particularly to those of the IS6 family. ISPsa2 carries its own promoter with standard −10 and −35 sequences, suggesting an interesting potential for plasticity in this pathogenic bacterium. Additionally, the presence of ISPsa2 was confirmed from three isolates of P. salmonis collected from different epizootics in Chile in 2010.
The sequencing of bacterial genomes from newly discovered species provides exciting opportunities to understand genome organization and evolution. In addition, it provides novel putative ORFs or potential coding sequences (CDSs) as well as signals for gene expression (Siguier et al., 2006). Most bacterial genomes are composed of a core minimal species backbone, but generally and for purposes of plasticity, they are complemented with other features such as mobile genetic elements (MGEs), which include bacteriophages, conjugative transposons, integrons, composite transposons and insertion sequences (ISs). These elements form part of an extensive gene pool that serves to promote gene exchange and reassortment (Craig et al., 2002). The IS elements are small, mobile, non-self-replicating DNA regions that specify only the gene(s) required for their transposition. In accordance with the features involved in the transposition process and the phylogenetic relationship between different transposases, they have been grouped into different families (Gartemann & Eichenlaub, 2001).
Among MGEs, bacterial ISs constitute the largest group, and they have been observed in most bacterial genomes and plasmids, where they may be present in large numbers (Mahillon & Chandler, 1998; Chandler & Mahillon, 2002). Indeed, in a large virulence plasmid of Shigella flexnery, an astonishing 153 (53%) ORFs are related to known and putative IS elements; no known bacterial plasmid has been described previously with such a high proportion of IS elements, and four new IS elements have been definitively identified (Venkatesan et al., 2001). Additionally, metagenomic sequencing has yielded a flood of bacterial genome data that confirm the presence of increasing numbers of mobile elements in all analyzed bacterial genomes. This has naturally led to the development of evolutionary studies where consistent IS annotation across many different genomes has become necessary, and several alternatives are now available for comparison and enhanced understanding of their evolutionary and functional roles (Siguier et al., 2006; Wagner et al., 2007).
Piscirickettsia salmonis is the etiologic agent of salmonid rickettsial septicemia, or piscirickettsiosis (Fryer et al., 1990), which is an aggressive infectious disease that has affected salmonid fish since the late 1980s (Bravo & Campos, 1989; Graggero et al., 1995; Marshall et al., 2007). Piscirickettsia salmonis is a facultative intracellular Gram-negative bacterium (Mauel et al., 2008; Mikalsen et al., 2008; Gómez et al., 2009) that was initially described as a Rickettsia-like obligate intracellular Alphaproteobacteria. Recently, it was reclassified as a Gammaproteobacteria that closely resembles Legionella and Francisella species (Fryer & Hedrick, 2003). This ambiguity misled researchers for more than a decade; therefore, its biology, epidemiology and genetics are almost totally unknown. Nevertheless, it is known that this bacterium persists in sea water (Olivares & Marshall, 2010), maintaining its infective potential under rough environmental conditions (Lannan & Fryer, 1994). This vitality suggests that its genetic background should be sufficiently versatile to adapt easily to changing stressful conditions. In fact, our laboratory has demonstrated that under limiting in vitro conditions, morphological and genetic changes are consistently observed (Rojas et al., 2008). Thus, the report of the first IS sequence in this genome strengthened the belief that the genome of P. salmonis might show a surprising degree of complexity and plasticity. As our laboratory can successfully grow this bacterium in liquid media (Gómez et al., 2009; E. González, F. Gómez, V. Henríquez, S. H. Marshall & C. Altamirano, unpublished data), based on increasing evidence of the adaptive potential of this bacterium (Rojas et al., 2008, 2009, 2010), we decided to evaluate the quality of the bacterial genome to determine whether the observed morphological changes and adaptability have a genetic background. This report constitutes the first evidence of a putatively larger complexity and potential for fluidity in the genome of P. salmonis.
Materials and methods
Growth conditions of P. salmonis
Piscirickettsia salmonis strain LF-89 (ATCC VR-1361) was maintained routinely on BCG agar plates (10 g L−1 tryptone, 5 g L−1 peptone, 5 g L−1 yeast extract, 5 g L−1 NaCl, 10 g L−1 glucose, 5% sheep blood and 1%l-cysteine) at 23 °C (modified from Mauel et al., 2008). A single bacterial colony was used to inoculate 25 mL of MC5 medium, and the inoculated medium was incubated at 23 °C and 100 r.p.m. of agitation. The composition of the MC5 culture medium will be published shortly (patent pending).
Three isolates of P. salmonis collected from Atlantic salmon (Salmo salar) during 2010 from different epizootics in Puerto Montt (Chile) were maintained on the CHSE-214 cell line (ATCC CRL-1681) as been described previously (Rojas et al., 2009). Monolayers of CHSE-214 cells were routinely propagated at 17 °C in 25 cm2 culture flasks containing minimal essential medium (MEM; Gibco), supplemented with 7.5% heat-inactivated fetal bovine serum and adjusted to pH 7.2 with 10 mM sodium bicarbonate and 15 mM HEPES.
DNA purification
Two-day-old P. salmonis LF-89 liquid cultures were centrifuged at 6000 g for 20 min, and genomic DNA was extracted using the AxyPrep™ Multisource Genomic DNA Miniprep Kit (AxyGen Bioscience) according to the manufacturer's instructions.
To obtain DNA from the three isolates of P. salmonis, 1 mL of 15-day-old supernatants from CHSE-214 infected cell line was centrifuged at 20 000 g for 15 min. The DNA from the resultant pellets was extracted using the Chelex-100 resin (BioRad) as described previously (Walsh et al., 1991).
The DNA concentration from all samples was determined by spectrophotometry using a Nanodrop-1000 and the DNA was kept at −20 °C until use.
Genomic library construction
A genomic DNA library of P. salmonis was constructed in the plasmid pBluescript SK (+) (Fermentas). Bacterial genomic DNA (3 μg) was partially digested with Sau3AI (New England Biolabs) for 30 min at 37 °C. The digestion reaction was stopped at 65 °C for 10 min. Following phenol : chloroform extraction and ethanol precipitation, the DNA was resuspended in 15 μL of nuclease-free water (IDT DNA Technologies). The pBluescript SK (+) vector was completely digested with the BamHI restriction enzyme (New England Biolabs) for 12 h at 37 °C and treated for 1 h with alkaline phosphatase (Promega) according to the protocol supplied by the manufacturer. Both digested DNAs were visualized by 1% agarose gel electrophoresis and stained with GelRed™ (Biotium). Finally, 600 ng of digested bacterial DNA and 300 ng of linearized pBluescript SK (+) vector DNA were ligated with T4 DNA ligase (Promega). The ligation mixture was used to transform Escherichia coli TOP10 cells by electroporation. The selection of transformants was performed on Luria–Bertani (LB) agar containing 50 μg mL of kanamycin (Sigma-Aldrich) in the presence of X-Gal (Promega). In total, 200 white colonies were analyzed by PCR using the M13-forward and M13-reverse primers. Colonies with an insert size greater than 500 bp were selected and grown in 5 mL of LB broth. They were purified using a Plasmid Mini Kit (Qiagen) and submitted to sequencing by Macrogen Inc. (Korea).
Sequence analysis
The DNA sequence data were analyzed with softberry server software (http://linux1.softberry.com/berry.phtml) using the FgenesB and Bprom algorithms. FgenesB is a suite of bacterial operon and gene prediction programs and is based on Markov chain models of coding regions and translation and termination sites (Tyson et al., 2004). Bprom is an algorithm that recognizes possible promoters in bacterial DNA sequences. The clc main workbench 5 is a versatile software for analyzing DNA, RNA and proteins with a graphical user interface (http://clcbio.com/); the software was used to complement the sequence analysis, specifically for alignments and to locate the different elements [ORF, promoters, inverted repeat sequences (IRs)].
The ORFs predicted by FgenesB were used in blastp, with the search limited to bacterial sequences (http://blast.ncbi.nlm.nih.gov), to determine their possible identities. A comparison with the most similar ISs from the IS6 family found in the ISFinder database (http://www-is.biotoul.fr/) was performed.
Determination of the presence of IS from fish isolates of P. salmonis
In order to determine the prevalence of the IS sequence in natural isolates, oligonucleotide primers were designed to amplify the putative IS already predicted by the sequence analysis. All PCR primers were designed as shown in Table 3, using the Oligo Calc tool (http://www.basic.northwestern.edu/bio-tools/oligocalc.html).
|Primer name
|IR right
|Tnp-Psa nt 1–24
|Tnp-Psa nt 682–704
|IR left
The PCR reaction for the three fish isolates was performed using the following primer set: (1) IR1-F and Tnp-PsaR2 yielded a PCR product of 427 bp and (2) Tnp-PsaF and IR2-R yielded a PCR product of 704 bp. The PCR conditions used were: 94 °C for 5 min, 35 cycles of 94 °C for 30 s, 58 °C for 30 s and 72 °C for 45 s, and a final extension of 72 °C for 5 min. The PCR products were visualized on a 1% agarose gel stained with GelRed™.
Genomic library construction
Piscirickettsia salmonis DNA was partially digested with Sau3AI endonuclease. Because this enzyme has a 4-bp recognition site, excision occurs, on average, every 250 bp, thus generating DNA fragments smaller than 2000 bp (Fig. 1). Fragmented DNA was cloned into the vector pBluescript KS (+) and electroporated into E. coli, resulting in 4750 recombinant clones. PCR analysis of the cloned P. salmonis inserts yielded 200 clones with inserts larger than 500 bp, which were subsequently sequenced (data not shown).
Sequence analysis
Sequence analysis of the 992-bp insert resulted in a unique 726-bp ORF with a putative in-frame protein of 242 amino acids, an upstream putative promoter containing the expected −10 and −35 regions, and two identical 16-bp IRs flanking the 726-bp ORF (Fig. 1).
According to Blastp analysis, the new ORF encodes a putative transposase (Tnp-Psa) with high similarity to Bacillus thuringiensis IS240 protein. The alignment with IS240 and other transposases showed a high percentage of identity (almost all >45%) (Table 1) and also indicated the presence of amino acids involved in the DDE motif (Fig. 2), an acidic aminoacid triad present in many phosphoryltransferases, important in catalysis reactions possibly involved in metal coordination; these residues are conserved in ISs of the IS3 and IS6 families (Mahillon & Chandler, 1998) (Table 1 and Fig. 2).
|GenBank accession no.
|Bacillus thuringiensis
|Bacillus cereus CER484
|Bacillus weihenstephanensis
|Bacillus thuringiensis ssp. israelensis (p112 kb)
|Bacillus thuringiensis ssp. israelensis (p112 kb)
|Staphylococcus aureus (pSH6)
A comparison with similar ISs, such as those of the IS6 family reported in ISFinder, showed a close relationship with some insertion elements from the genera Bacillus and Staphylococcus, even at the nucleotide alignment level (data not shown). Our new IS, ISPsa2 (GenBank accession number: HM563000), shares key features with these sequences (Table 2).
|GenBank accession no.
|Piscirickettsia salmonis
|Bacillus thuringiensis
|Bacillus cereus CER484
|Bacillus weihenstephanensis
|Bacillus thuringiensis ssp. israelensis (p112 kb)
|Bacillus thuringiensis ssp. israelensis (p112 kb)
|Staphylococcus aureus (pSH6)
Prevalence of ISPsa2 from fish isolates
In order to determine the prevalence of the ISPsa2 sequence in fish isolates, we tested its presence in three fresh isolates, amplifying the IS by PCR using two sets of ISPsa2-specific primers (Table 3). The ISPsa2 sequence was found in the genome of all three isolates from fish (Fig. 3).
The genomes of a large number of bacterial species have been sequenced in the last decade, generating important data for comparative analyses. Comparisons of the sequences and organization of these different genomes reveal interesting biological and evolutionary information. The recent development of an open-source software package called iscan has enabled the identification of a wide array of bacterial ISs and their sequence elements (Wagner et al., 2007) as well as their systematic classification (Siguier et al., 2006). Such analyses substantially expand upon previously available information and suggest that most ISs have entered bacterial genomes recently. By implication, the persistence of their populations may depend on horizontal transfer, a highly important issue in salmon rearing, where fish confinement and stress are commonplace situations at critical times before harvesting. Under such conditions, ISs and other MGEs associated with pathogenesis could become particularly active as part of a bacterial strategy to maintain its virulence. Additionally, the presence of ISs might also very well be the starting point to generate more complex mobile units, such as transposons, which undoubtedly provide advantageous conditions for survival to pathogenic bacteria. Indeed, as supportive evidence, bacterial genomes are known to be remarkably fluid (Boucher et al., 2003). A fluid genome represents a huge advantage for all prokaryotes, more so for pathogens, enabling quick adaptation to harsh ecological niches and to diverse environmental selective pressures. Most of these sudden changes are generally mediated by lateral gene transfer strategies in which MGEs play a pivotal role, reinforcing the notion that a substantial portion of the bacterial genome is not inherited from the parental cells, but is instead acquired horizontally by lateral gene transfer (Doolittle, 1999; Boucher et al., 2003). The presence of ISs should favor the acquisition of new traits through this mechanism as a way to allow bacteria to rapidly adapt to new environmental conditions, a proinfective behavior that fits perfectly with the rough natural environment faced routinely by P. salmonis. In this context, and considering that key virulence genes that distinguish pathogenic bacteria are generally carried on transmissible genetic elements (Hacker et al., 1997), it would not be surprising if the genomic complexity of P. salmonis included other types of MGEs, a feasible alternative that our laboratory is currently investigating.
In summary, this is the first description of a putatively functional IS in the genome of P. salmonis. Our results reveal that ISPsa2 shares high similarity to previously described ISs – specifically to IS240 elements, which are members of the IS6 family. As shown in Table 2, our new IS shares the key features that distinguish the IS6 family elements, such as length, IR size and END sequence. The putative transposase encoded within ISPsa2 (Tnp-Psa) carries conserved motifs that are also found in other transposases (Fig. 2). The presence of a putative promoter region in frame with Tnp-Psa in ISPsa2 strongly suggests a regulated self-expression for the IS and may represent a preliminary indication of the high genomic plasticity of this fish bacterial pathogen. Additionally, the ISPsa2 sequence appears to be in other strains of the pathogen, or at least in three isolates obtained from epizootics in 2010 (Fig. 3).
This work was supported by Innova Corfo grant 05CT6IPD-22 to S.M., C.C. and V.H. and by Conicyt (Beca Nacional de Doctorado) to F.G.
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Original Research ARTICLE
Efficient foot motor control by Neymar's brain
- 1Center for Information and Neural Networks (CiNet), National Institute of Information and Communications Technology, Suita, Japan
- 2Graduate School of Medicine and Graduate School of Frontier Biosciences, Osaka University, Suita, Japan
How very long-term (over many years) motor skill training shapes internal motor representation remains poorly understood. We provide valuable evidence that the football brain of Neymar da Silva Santos Júnior (the Brasilian footballer) recruits very limited neural resources in the motor-cortical foot regions during foot movements. We scanned his brain activity with a 3-tesla functional magnetic resonance imaging (fMRI) while he rotated his right ankle at 1 Hz. We also scanned brain activity when three other age-controlled professional footballers, two top-athlete swimmers and one amateur footballer performed the identical task. A comparison was made between Neymar's brain activity with that obtained from the others. We found activations in the left medial-wall foot motor regions during the foot movements consistently across all participants. However, the size and intensity of medial-wall activity was smaller in the four professional footballers than in the three other participants, despite no difference in amount of foot movement. Surprisingly, the reduced recruitment of medial-wall foot motor regions became apparent in Neymar. His medial-wall activity was smallest among all participants with absolutely no difference in amount of foot movement. Neymar may efficiently control given foot movements probably by largely conserving motor-cortical neural resources. We discuss this possibility in terms of over-years motor skill training effect, use-dependent plasticity, and efficient motor control.
Keywords: Neymar da Silva Santos Júnior, football brain, foot movement, medial-wall motor region, functional magnetic resonance imaging, efficient motor control, long-term physical training
Citation: Naito E and Hirose S (2014) Efficient foot motor control by Neymar's brain. Front. Hum. Neurosci. 8:594. doi: 10.3389/fnhum.2014.00594
Received: 14 May 2014; Accepted: 16 July 2014;
Published online: 01 August 2014.
Edited by:Sven Bestmann, University College London, UK
Reviewed by:Eva Feredoes, University of Reading, UK
Joseph M. Galea, University of Birmingham, UK
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*Correspondence: Eiichi Naito, Center for Information and Neural Networks (CiNet), National Institute of Information and Communications Technology, 2A6, 1-4 Yamadaoka, Suita, 565-0871, Japan e-mail: firstname.lastname@example.com


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Defensive End
Height: 6-foot-4
Weight: 265 pounds
Class Year: 2V
Hometown: Hawthorne, CA
Prior School: El Camino JC
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The Latest: Grading the defense — 10/23/2010
Utah's defense has been outstanding through the first six games, despite entering the season with the most questions and the most to prove. Utah is ninth nationally in scoring defense, allowing just 14 points per game. Utah's defense ranks in the top 15 in run defense, pass defense, total defense,...[details]
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2009 (Junior): Saw action in nine games ... nine total tackles ... two tackles with a half-sack vs. UNLV ... a three-yard sack against Wyoming ... also had two tackles vs. Air Force and Cal in the Poinsettia Bowl.
2008 (Sophomore): Played in four games ... two tackles included an assisted tackle for loss ... lettered.
Personal: Played for El Camino JC in
originally signed with Idaho out of high
first-team all-conference tight end
lettered three years at Leuzinger High (Calif.)
also lettered in volleyball, basketball and
all-area in volleyball
Leuzinger High
MVP led team to the Ocean League volleyball
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sociology major
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Five things we learned — 9/3/2010, from UteZone.com
Red, white, black and ... yellow? — 8/25/2010, from UteZone.com
Shifting gears — 8/18/2010, from UteZone.com
Cox talks leadership — 8/18/2010, from UteZone.com
Fighting through the wall — 8/12/2010, from UteZone.com
Utah defense looks to fill holes — 8/5/2010, from UteZone.com
Position battles: front seven — 8/3/2010, from UteZone.com
Preseason 120 Countdown: No. 26 Utah — 7/25/2010, from Rivals.com College Football
Breaking down the defensive line — 5/25/2010, from UteZone.com
Utah defense looks to finish spring strong — 3/22/2010, from UteZone.com
Day One Notes: Defense — 3/9/2010, from UteZone.com
Spring Guide to the Defensive LIne — 3/8/2010, from UteZone.com
Spring Preview: Defense — 2/11/2010, from UteZone.com
Utes employ new strategy — 8/20/2009, from UteZone.com
Ute defense alive and kicking — 8/12/2009, from UteZone.com
Offense shows signs of life — 8/8/2009, from UteZone.com
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White Papers propose research-based solutions to emerging issues in English age teaching and learning in contexts around the world.
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This is a printed book. It is also available in PDF format .Grammar is a component in all language skills: reading, writing, speaking, and listening. Teachers need to know rules of grammar (teacher knowledge) as well as techniques that help students use grammar effectively and effortlessly (teaching knowledge). Using reflective practice to help teachers become comfortable with teaching grammar, this easy-to-follow guide defines grammar from a teacher perspective, shows how corpus linguistics can be a useful tool, covers basic concepts of grammar research, and addresses important components of teaching grammar. Teaching Grammar is a valuable addition to the literature in our profession.
About the English Language Teacher Development Series, Thomas S. C. Farrell, Series Editor: What is the latest research, and how do I use it in my classroom? The pressure is intense for language teachers to update their knowledge in curriculum trends, research, theory and practice, technology, or assessment. The need to update is certainly not a reflection of inadequately trained teachers but is due to the nature of a profession that continuously reinvents itself. The English Language Teacher Development Series is a set of short resource books written in a jargon-free and accessible manner for all types of teachers of English (native and nonnative speakers of English, experienced and novice teachers). The Series offers a theory-to-practice approach, a variety of practical methods, and time for reflections that allow teachers to interact with the materials presented. The books can be used in preservice settings, in-service courses, and by individuals looking for ways to refresh their practice.
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The CCSS in Mathematics for Grades K-8
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This is a printed book. It is also available in an eBook format for Kindle and Apple and Android.
The CCSS have been adopted by most states in the country and we as mathematics educators must attend to this widespread adoption and implementation.
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This is a printed book. It is also available in an eBook format for Kindle and Apple and Android.This book accelerates the process of learning English language teaching by offering a nontechnical introduction to ELT geared toward native-English-speaking teachers working outside their home countries.
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- 3 Things To Do Today
- » Gain the right amount of pregnancy weight
- » Learn the differences between boys & girls
- » Find the best potty seat
Rainbow-Themed Birthday Party Ideas
When WhatToExpect.com community member, mama odyssa, was considering first birthday party ideas for her daughter, Kate, she knew she wanted a day filled with bright color, cheerfulness, and plenty of food that would delight her pint-sized party guests.
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Rainbow Jello dessert requires patience — but it's definitely worth it in the end.
This Rainbow M&M birthday cake is exploding with color and makes for the perfect "smash cake."


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OneNote for one special wedding day
One bride-to-be, Ambir, had an unexpected obstacle come up during her wedding plans–her fiancé, George, was transferred 300 miles away. Luckily, Ambir was using OneNote for her wedding planning so she was able to stay organized and keep George involved throughout the process. Here are some of her suggestions for planning your big day!


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i was inspired by the hobbit movie. and i love galadriel. so yeah >.<
love the colors in this!!! ♥
she looks so beauty! >u<
I love it. Any chance I could buy it ?
I really like the colours! :3
it looks amazing! >3<
it looks amazing! >3<
Great color choices!
Stunning work. I love her too.
Omg! Wonderful >.<
I love the Hobbit
I love the Hobbit


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Corvallis 104.3 FM • Hood River 91.9 FM • Portland 90.7 FM
For years, people used to migrate into the united states through the cities.
Then in the early 1990's trade policies began to effect patterns of immigration, . The US began building walls, and sealing urban areas, pushing immigration routes to isolated and more desolate areas, like the desert.
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Play is the building block of childhood. It teaches kids about their world. Here, learn what activities inspire and motivate, and which toys are not only safer, but smarter, too.
Note: All information is for educational purposes only. For specific medical advice, diagnoses, and treatment, consult your doctor.
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Since the day his family's ranch was stolen by Fletcher Austin, Ramon de la Guerre vowed to find a way to reclaim his birthright. Riding the California hills as the mysterious, avenging "El Dragon," he and his men strike out at the Americanos.
Caralee (Carly) McConnell had come to California believing that here she could begin a new and wonderful life free from poverty. But once she met the handsome Ramon and sensed the hostility between her Uncle Fletcher and the virile young Spaniard, Carly knows she is in the middle of a war.
When fate places Carly in Ramon's hands, he plans to make her life a living hell, but her innocence and sweetness soon melt his resolve and in his mountain hideout passion begins to grow.
Though Ramon sets her free, Carly can never forget the love she has for the desperado and rather than being forced into marriage, she weds Ramon, pledging to help him find a way to regain his home and his freedom.
Drawing on the colorful history of her native California, Kat Martin weaves a marvelous western romance that sizzles with unbridled passion and a heated battle of wills. Readers will be enraptured by the heart pounding adventure and warmed to their toes by the sensuality of MIDNIGHT RIDER. VERY SENSUAL (Mar., 372 pp., $5.99)


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Coronary Artery Disease Medications
Definition of Coronary Artery Disease:
Coronary Artery Disease is a narrowing or blockage of the small blood vessels that supply blood and oxygen to the heart. It is caused by atherosclerosis,a buildup of plaque in the arteries to your heart.
Drugs associated with Coronary Artery Disease
The following drugs and medications are in some way related to, or used in the treatment of Coronary Artery Disease. This service should be used as a supplement to, and NOT a substitute for, the expertise, skill, knowledge and judgment of healthcare practitioners.
|Drug Name (View by: Brand | Generic)
|Norvasc (Pro, More...)
generic name: amlodipine class: calcium channel blocking agents
|0 reviews
|Not rated
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|Aceon (Pro, More...)
generic name: perindopril class: angiotensin converting enzyme inhibitors
|0 reviews
|Not rated
Be the first
|Voxsuprine (More...)
generic name: isoxsuprine class: peripheral vasodilators
|0 reviews
|Not rated
Be the first
|Vasodilan (Pro, More...)
generic name: isoxsuprine class: peripheral vasodilators
|0 reviews
|Not rated
Be the first
See 6 generic medications used to treat this condition
Alternative treatments for Coronary Artery Disease
The following products are considered to be alternative treatments or natural remedies for Coronary Artery Disease. Their efficacy may not have been scientifically tested to the same degree as the drugs listed in the table above. However there may be historical, cultural or anecdotal evidence linking their use to the treatment of Coronary Artery Disease.
Topics under Coronary Artery Disease
- Acute Coronary Syndrome (7 drugs in 2 topics)
- Coronary Arteriography (13 drugs in 3 topics)
Learn more about Coronary Artery Disease
Micromedex® Care Notes:
Symptoms and treatment for:
Drugs.com Health Center:
Mayo Clinic Reference:
Synonym(s): Arteriosclerotic Heart Disease; CAD; CHD; Coronary Heart Disease


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K Swiss Spliced Crew
Every male tennis player needs a go-to crewneck and this Spliced Crew by K-Swiss is just that. It features mesh fabric at heat-prone areas, lightweight fabric that wicks moisture away from the skin, and material that offers SPF 50+ UV protection. K-Swiss logo at left chest.
Color: White/Stingray Fabric: 100% polyester pique Mesh: 100% polyester


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Hard Candy So Baked Bronzer - Tiki
125 reviews
88% would repurchase
Package Quality: 4.1
Price: $$
Package Quality: 4.1
Price: $$
INGREDIENTSMica, Talc, Mineral Oil, Dimethicone, Magnesium Aluminum Silicate, Phenoxyethanol, Methylparaben. May Contain ( / -): Titanium Dioxide (CI 77891), Iron Oxides (CI 77491, CI 77492, CI 77499)
Age: 25-29
Skin: Oily, Medium, Warm
Hair: Brunette, Wavy, Medium
Eyes: Brown
Nc 30-35, warm and yellow undertones
This color is frickin gorgeous!!! It's a shimmery golden-beige color that looks gorgeous on any skin tone. I've used this when I was pale and tan, and it looked lovely either way. I don't know why it's listed as a bronzer because this shade is sooooo light. This has been my favorite highlighter lately. On lazy days, ill use this as an eyeshadow; it brightens up my eyes!
Age: 30-35
Skin: Very Dry, Fair-Medium, Warm
Hair: Blond, Wavy, Fine
Eyes: Green
Like Reserve Your Cabana bronzer from Wet n Wild, everyone uses this bronzer as a highlighter as well, and again, I don't quite get it. It's just a bit too matte for a highlighter for me. The shade is just a bit too golden as well and ends up looking obvious and unnatural on me. Oh well, to each his own.
Age: 25-29
Skin: Oily, Other, Not Sure
Hair: Brown, Other, Other
Eyes: Hazel
this is most definitly not a bronzer, it works extremely well as a highlighter though. I wanted something that didn't have any obvious shimmer in it. this goes on easily and blend well. it looks really natural on the skin. it's something that I have used almost every time I wear makeup and it has lasted me about 5 months. I had to repurchase once bc the packaging broke (I hadn't dropped it that badly either so that's why I'm giving it four). this is better then the more expensive brands I have tried (benefit, urban decay, ect)
Age: 18 & Under
Skin: Combination, Medium, Not Sure
Hair: Brunette, Other, Other
Eyes: Brown
HG product! Nc 40 skin makes it looks golden and dewy love it.
Age: 25-29
Skin: Acne-prone, Fair, Warm
Hair: Brunette, Wavy, Fine
Eyes: Brown
This is my absolute HG highlighter.
As others have said, it is *definitely* not a bronzer. I am light and this doesn't make me look remotely tan lol
What it *does* do is add am amazing glow to my skin, it is everything I wanted the much more expensive Nars copa, or Benefit moonbeam to be.
For starters I prefer that it is in powder form, I find it way easier to work with. Also I know this product will last forever. I have been wearing it daily for over a month and it still looks new. It is *not* shimmery, which is great. It doesn't draw attention to my large pores, make my skin look oilier, or make me look like a disco ball. It just blends into my skin, giving it this healthy glow. It's super natural, easy to work with, and also very forgiving.
When I apply it, I use a small blush brush, and I make little "c"s to highlight my cheekbones, then i apply to my nose as well. After I may apply blush or bronzer, but if I want to look natural I just leave it as is. And the look is so natural and flawless.
The only con, is that I wish the compact had a mirror. Other than that it's perfect.
Age: 19-24
Skin: Combination, Fair, Neutral
Hair: Blond, Wavy, Medium
Eyes: Hazel
Works perfect as a highlight color, especially in the summer with a bit of a tan
Goes a long way since you just need a small swipe on your brush - it is a bit shimmery, but perfect for summer paired with a matte bronzer
Age: 30-35
Skin: Combination, Medium, Not Sure
Hair: Black, Other, Other
Eyes: Brown
Works better as a highlighter than a bronzer. I use Samantha Chapman's real techniques setting brush to apply the powder onto my cheekbones. It's reasonably priced and available at Walmart.
Age: 19-24
Skin: Combination, Fair, Not Sure
Hair: Brown, Other, Other
Eyes: Blue
I've bought this a few times now and will continue to buy. I love it! I prefer a more sunkissed/golden look over blush, but being as I'm soo light skinned it's always been a little harder to find something that could do that for me without looking just awful. It also gives a nice glowy look without looking shimmery and silly. I definitely grab for it every morning; it just gives the perfect finishing touch.
Age: 30-35
Skin: Combination, Fair-Medium, Warm
Hair: Blond, Straight, Fine
Eyes: Hazel
I love this as a golden highlighter. In the summer I really like to shelf my silvery highlights and play with the sunny golden ones. This is a great affordable highlighter. I sweep this over my cheekbones, down the bridge of my nose and across my cupids bow. Love the glimmer it leaves. The shimmer is not chunky, you are just left with a nice glow. Will repurchase if I ever run out.
Age: 19-24
Skin: Dry, Fair, Not Sure
Hair: Black, Other, Other
Eyes: Green
Love this product as a highlighter, definitely not a bronzer. I'm an NC15 and its a beautiful color for me. I sometimes reach for this more than my Mary lou-manizer from the balm. Would definitely repurchase, this product will last me for a very long time! Definitely worth the price.
Popular Bronzers
1286 Reviews
448 Reviews
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1274 Reviews


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good job
в промзонах есть какое-то очарование...
Could be a TinTin scene. I like it
Way to go with the perspective and the industrial feel. I find both very tricky!
Simple it may be, but very easy in the eyes nevertheless.
Reminds me of the illustrations in some older manuals and guides. Simple but direct. Well done.
sweet job!


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Medical library
A health or medical library is designed to assist physicians, health professionals, students, patients, consumers, medical researchers and information specialists in finding health and scientific information to improve, update, assess or evaluate health care. Medical libraries are typically found in hospitals, medical schools, private industry and in medical or health associations. A typical health or medical library has access to MEDLINE, a range of electronic resources, print and digital journal collections and print reference books. The influence of open access (OA) and free searching via Google and PubMed has a major impact on the way medical libraries operate.
To become accredited, every American and Canadian college of medicine, nursing, dentistry, pharmacy, veterinary medicine or public health is required to have a health or medical library appropriate to the needs of the school, as specified by an accrediting body, such as the Liaison Committee on Medical Education (LCME)'s standards. These accreditation standards include having qualified library staff on hand to answer reference questions, and provide training in using electronic resources. Some academic medical libraries are located in the same building as the general undergraduate library but most are located near or in the medical college or faculty.
The United States National Library of Medicine (NLM) is the largest biomedical library in the world, and collects and provides access to some of the best health information in the world (due to its linkage to the National Institutes of Health). The NLM maintains numerous medical and genomic databases, searchable via its Entrez search system, including MEDLINE (PubMed) and OMIM (a genetic traits database).
In support of open access to the journal literature, the U.S. NLM established an online library of digital journal articles, PubMed Central (PMC), which will soon be supplemented by a UK version. NLM works with the National Network of Libraries of Medicine (NN/LM) to provide regional medical library support in the United States, while its consumer health information service MEDLINEplus offers free access to health information, images and interactive tutorials. Many countries like Australia, Canada and the United Kingdom have well-developed medical libraries, though nothing quite as evolved as the U.S. NLM.
The largest medical library in Europe is the German National Library of Medicine (ZB MED), which also has collections in the fields of nutrition, agriculture, and environmental science. ZB MED operates as the official European supplier of full texts in response to searches conducted in the NLM's bibliographic databases such as PubMed, and also operates its own search portals.[1]
Medical librarians[edit]
Medical librarians are skilled professionals who assist with resources and research in the medical professions. The focus of the medical librarian is to emphasize the use of evidence based research and practice. This can be for both medical research or medical practice. As well the medical librarian is expected be a resource for assisting with publishing and presentation of research. Medical librarians use web based resources to conduct research and help generate evidence based approaches to healthcare. Organizations such as the MLA set standards for what a medical librarian should follow in practice.[2]
The Medical Library Association (MLA) is a Chicago-based advocate for library professionals and health sciences libraries - primarily in the United States. MLA maintains an online list of ALA-accredited library school programs for those who would like to pursue a master's degree in library and information studies in the US and Canada(MLIS). It furthermore administers the U.S credentialing organization for medical librarians, the Academy of Health Information Professionals(AHIP).
The Special Libraries Association has a Medical Section of the Biomedical and Life Science Division, which serves as a forum for Division members who are engaged or interested in the exchange of information in the biomedical and health sciences, and the acquisition, organization, dissemination, and use of such information in all formats.
In Canada, health librarians and libraries are represented by the Canadian Health Libraries Association.
Australia have the Health Libraries Australia Group of the Australian Library and Information Association and the Victorian state based Health Libraries Inc. In 2013, HLA introduced a PD Specialisation in 2013 leading to Association post nominals on demonstration of ongoing professional development in the field of health librarianship. A list of health libraries in Australia may by found on the website of the National Library of Australia. In 2015, Australia is undertaking a Census of health libraries and librarianship aiming to identify the full industry, locations, organisations, roles and staff employed. The first specialist "Health Librarianship Essentials" tertiary training commenced in April 2015 at the Queensland University of Technology supported by HLA.
In the United Kingdom medical (or health) librarians are represented by the Health Libraries Group of the Chartered Institute of Library and Information Professionals. CILIP. Health Libraries Group.
The medical and health libraries of the German-speaking countries Germany, Austria and Switzerland are represented by the Medical Libraries Association Arbeitsgemeinschaft fuer Medizinisches Bibliothekswesen (AGMB) e.V..
There are similar, if smaller, national groups in many European countries and these groups and individual health librarians and libraries are represented by the European Association for Health Information and Libraries (EAHIL) since 1987.
For those librarians who work in very specialised subject areas there are associations and networks which focus on their subject areas, and an example of such an organisation is Elisad, the European Association of Libraries and Information Services on Addictions. Elisad
The International Federation of Library Associations and Institutions (IFLA) has a Health and Biosciences Libraries Section. The last International Congress on Medical Librarianship (ICML) was in Brisbane in 2009, the next ICML will be in Boston, 2013.
US & World Medical Library Links[edit]
- California Pacific Medical Center/Univ Pacific School Of Dentistry (San Francisco)
- Cedars-Sinai Medical Center (West Hollywood)
- Drew University Of Medicine and Science
- Loma Linda University
- Lane Medical Library
- Stanford Hospital Health Library
- St. John's Regional Medical Center (Oxnard)
- St. Joseph Hospital (Orange)
- University Of California/Berkeley Optometry Library
- University Of California/Berkeley Public Health Library
- University Of California/Davis
- University Of California, Irvine, Grunigen Medical Library
- University Of California/Los Angeles
- University Of California/San Diego
- University Of California/San Francisco
- UCSF Mount Zion Medical Center
- University Of Southern California
- USC Wilson Dental Library
- USC Gerontology Center Library
- Medical Library Group Of Southern California & Arizona
- Hartford Hospital
- University of Connecticut Health Center
- Yale University, Cushing/Whitney Medical Library
District Of Colombia
- Catholic University of America - Nursing/Biology Library
- George Washington University
- Georgetown University Medical Center
- Howard University
- Pan American Health Organization
- Florida International University
- Florida State University
- Nova Southeastern University Health Professions Division Library (Fort Lauderdale)
- University of Florida/Gainesville
- University of Miami
- University of South Florida
- Eisenhower Army Medical Center/SE Regional Medical Command (Fort Gordon)
- Emory University
- Georgia Health Sciences Library Association
- Mercer University
- Philadelphia College of Osteopathic Medicine - Georgia Campus
- American Dental Association
- Health Science Librarians of Illinois
- Loyola University
- Northwestern University
- OSF Saint Francis Medical Center (Peoria)
- Rosalind Franklin University (Chicago)
- Rush University (Chicago)
- Southern Illinois University
- University of Chicago
- University of Illinois/Chicago
- University of Illinois/Peoria
- University of Illinois/Rockford
- University of Illinois/Urbana
- Indiana University Medical Library
- Indiana University Dentistry Library
- Purdue University (Pharmacy, Nursing, Health Sciences)
- Ochsner Clinic Foundation (New Orleans)
- Louisiana State University Health Sciences Center/New Orleans
- Louisiana State University/Shreveport
- Tulane University Medical Library
- Harvard Medical Web
- Countway Library of Medicine
- Massachusetts General Hospital
- New England College od Optometry
- South Coast Health System (New Bedford)
- Tufts University
- University of Massachusetts
- Botsford General Hospital (Farmington Hills)
- Henry Ford Health System (Detroit)
- Providence Hospital (Southfield)
- Synergy Medical Education Alliance (Saginaw)
- University of Michigan
- Wayne State University
- Metropolitan Detroit Medical Library Group
- Kirksville College of Osteopathic Medicine
- Saint Louis University
- University of Missouri
- University of Missouri/Kansas City
- Washington University
New Hampshire
New Jersey
- Medical Center at Princeton
- Monmouth Medical Center (Long Branch)
- St. Peters Medical Center (New Brunswick)
- University of Medicine and Dentistry of New Jersey
New Mexico
New York
- Albany Medical College
- Albert Einstein College of Medicine
- Columbia University
- Columbia University Health Sciences Library
- Cornell University
- Kaleida Health Libraries (Buffalo)
- Mount Sinai
- New York Academy of Medicine
- New York Dept of Health (Albany)
- New York Medical College
- New York Methodist Hospital (Brooklyn)
- New York University
- Rockefeller University
- St. Luke's Cornwall Hospital (Newburgh)
- State University of New York/Buffalo
- State University of New York/New York (College of Optometry Vision Science Library)
- State University of New York/Stony Brook
- State University of New York/Upstate-Syracuse
- University of Rochester
- Winthrop University Hospital (Mineola)
North Carolina
- Duke University
- East Carolina University
- National Institute of Environmental Health Sciences (NIH)
- University of North Carolina
- Wake Forest University
North Dakota
- Cleveland Health Sciences Library, Case Western Reserve
- Northeast Ohio Medical University
- Ohio State University
- University of Cincinnati
- Drexel University (Philadelphia)
- Easton Hospital
- Fox Chase Cancer Center (Philadelphia)
- Pennsylvania State University (Hershey Medical Center)
- Thomas Jefferson University
- University of Pennsylvania
- University of Pittsburgh Health Sciences Library System
South Carolina
South Dakota
- East Tennessee State University
- St. Jude Children's Research Hospital (Memphis)
- University of Tennessee Medical Center, Knoxville
- University of Tennessee, Memphis
- Vanderbilt University (library)
- Baylor Health Sciences Library
- Houston Academy of Medicine-Texas Medical Center
- Texas A & M
- Texas Dept of State Health Services (Austin)
- Texas Tech University
- University of North Texas-Fort Worth Health Sciences Center
- University of Texas Health Center at San Antonio
- University of Texas Health Center,Tyler
- University of Texas M.D. Anderson Cancer Center, Research Medical Library
- University of Texas Medical Branch, Galveston
- University of Texas/Southwestern
- Overlake Hospital (Bellevue)
- University of Washington Health Sciences Library
- University of Washington Dept of Environmental Health
- Washington Medical Librarians Association
West Virginia
- Medical College of Wisconsin
- St. Joseph Hospital/covenant Library System, Milwaukee
- University of Wisconsin
US Territories
- Canberra Hospital
- Flinders University/Medical Library (South Australia)
- Monash University/Biomedical Library (Victoria)
- Royal Victorian Eye and Ear Hospital/Royal Australian College of Ophthalmologists
- University of Melbourne-Biomedical Library
- University of New South Wales/Biomedical Library
- University of Queensland/Biological & Health Sciences Libraries
- University of Sydney
- Women's and Children Health (Melbourne)
- Dalhousie University (Halifax, Nova Scotia)
- Health Science Information Consortium of Toronto
- McGill University
- McMaster University
- Memorial University of Newfoundland
- University of Alberta
- University of British Columbia
- Children's/Women's Hospitals
- University of Calgary
- University of Manitoba
- University of Montreal
- University of Ottawa
- University of Saskatchewan
- University of Toronto
- University of Western Ontario
- Freie Universitaet Berlin
- German National Library of Medicine, Koeln
- Universitaats- und Landesbibliothek, Muenster, Zweigbibliothek der Medizin
- Saarlaandische Universitaats-und Landesbibliothek-Medizinische Abteilung, Homburg/Saar
- Universitaat Magdeburg, Medizinische Zentralbibliothek
United Kingdom
- Bedford Hospitals NHS Trust
- British Medical Association
- Cardiff University
- Health Libraries Group
- National Health Service (London)
- Royal College of Nursing
- South West Health Care
- Hope Hospital / University of Manchester
Other Countries
- American University of Antigua (West Indies)
- Medical University of Sofia Central Medical Library (Bulgaria)
- Chinese University of Hong Kong (China)
- Zagreb University (Croatia)
- TerKko – National Library of Health Sciences(Finland )
- Helsinki University – Dental Library
- medicale.tr List of French Medical libraries, from Rouen University Hospital Medical Library
- SBC institute (Athens, Greece)
- National University of Ireland, Galway
- Hebrew University (Israel)
- Kuwait University
- Kuala Lumpur Hospital (Malaysia)
- Academic Hospital Groningen (The Netherlands)
- Hospital de Cruces (Bilbao, Spain)
- Hospital Ramon y Cajal Biblioteca (Madrid)
- University of the West Indies, St Augustine (Trinidad)
- Rashid medical library (United Arab Emirates)
See also[edit]
- Medical Library Association
- Medical Library Association of Great Britain and Ireland
- Canadian Health Libraries Association
- Margaret Ridley Charlton
- Fred Kilgour
- United States National Library of Medicine
- Osler Library of the History of Medicine
- Houston Academy of Medicine-Texas Medical Center Library
- Wellcome Library
- ^ German National Library of Medicine Partnerships (in English) retrieved 23-May-2012
- ^ Roper FW (Jan 2006). "The Medical Library Association's professional development program: a look back at the way ahead" (PDF). J Med Libr Assoc 94 (1): 8–18. PMC 1324767. PMID 16404465.
|Wikimedia Commons has media related to Medical libraries.
ais.ubc.ca/ HLWIKI International — A Knowledge-Base for Health Librarians]


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Kevin S.Twitter Stats
@kevin - Tracking since September 26, 2008
ceo & cofounder at instagram
San Francisco, CA.
Kevin S. Tweets
@kevin has posted 0 tweets in the last 30 days, which translates to an average of 0.0 tweets per day.
Kevin S. Followers
@kevin has 79,709 followers.
This account is #72,753 in the worldwide rank of the most popular Twitter users.
Kevin S. Following
@kevin is following 52 Twitter accounts.
Last month this account followed no users.
Kevin S. Predictions & Milestones
@kevin will hit 81,200 followers in the next 3 months, and 85,757 in one year.
You are on Kevin S.'s Twitter stats page
We track these Twitter stats since September 26, 2008 . You can see how many followers Kevin S. lost or gained and what the prediction is for tomorrow or the next 15 days, together with all kinds of other stats like rank compared to all Twitter users, tweets etc.
Why don't you give it a try and compare Kevin S. to yourself ? Or if you are Kevin S. get even more feature rich & up-to-date stats with Premium & Pro Twitter Stats.
Twitter Counter is the #1 Twitter stats site powered by Twitter. It all started in June 12, 2008 and we grew rather rapidly because of our Twitter Counter badge. The button is a nice small image that shows your visitors how many followers you have on Twitter. Within a year the button was on many many blogs around the world and we displayed more than 100 million buttons in one month. We track follower stats for over 180 million users now.


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FBI: March 2014 Was Biggest March in Gun Background Check History
Fish & Game News April 14, 2014 Elliott
FBI released March's background check numbers to reveal "it was the biggest March in the National Instant Criminal Background Check (NICS) system's history."
According to Guns.com, the raw data shows "2,488,842 total background checks were preformed in March 2014."
This is up 200,000 checks over March last year and 400,000 checks over the number that took place in February.
March contained 10 days that were each among the highest in NICS checks history, "and the first two weeks of the month ranked in the top 10 for highest weeks for background checks."
The National Shooting Sports Foundation points out that when the when the raw data is adjusted to show the exponential background check jump in North Carolina, March goes from being the biggest March ever to the second biggest.
Source: Breitbart


text:
- Sandra Day O'Connor College of Law
- ASU Home
- My ASU
- Colleges & Schools
- Map & Locations
- Directory
This course will identify the sources for privacy protections in tort, statutory and Constitutional law. It then focus upon contemporary challenges arising from (1) government policies and (2) emerging technologies, in particular the increased use and functionality of electronic communications, encryptography and rapidly improving biotechnologies.
Additional Information:
Credit Hours: 3
Grading Option: Letter Grade Only
Graduation Writing Requirement: Yes, With Instructor's Approval*
Flexible Writing Requirement: Yes*
Skills Requirement: No
Note: Only one of the above listed requirements can be fulfilled with this course.
Final Exam Given: No
Attendance Policy: Per Statement of Student Policies
Online Course Site: None


text: No wifi. No laundry facilities. No forks!!! (The rest of the kitchen okay). Basic furnishings. Clean. Countryside is nice. But far enough outside of town to require lots of planning about what all would be needed for the day since commuting back and forth was a time-eater and inconvenient. Walls between bedrooms/baths paper thin. Would not stay here again, even... More


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Trying to conceive after mc? Pregnant after MC and seeking somewhere safe to hide? Recently graduated from the mosh pit? Come on down to the mosh pit for some serious metalling and cake part 5(315 Posts)
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DailyDirt: Getting Unwanted Carbon Dioxide Out Of The Atmosphere
from the urls-we-dig-up dept
- Carbon dioxide from the air can be sequestered into carbon nanofibers with electricity (preferably from renewable sources or some kind of nuclear). This development could help spur carbon nano-materials AND reduce carbon dioxide levels in our atmosphere. Win-win! (But it'll take a LOT of energy to make a dent in the existing amount of CO2 in the air.) [url]
- Removing carbon dioxide from the atmosphere might not be sufficient to reverse some of the damage already done to oceans and the ecosystem. Carbon dioxide levels have surpassed 400 ppm in the atmosphere, and some scientists have estimated that levels beyond 420 ppm would be detrimental to global ecosystems, as well as changing the climate around the world. [url]
- The Center for Carbon Removal wants to remove carbon dioxide from the atmosphere to clean up our environment. Carbon dioxide levels were at about 280 ppm before the Industrial Revolution, and they could continue to rise if no one changes our current energy infrastructure. [url]
- Turning carbon dioxide into useful materials isn't a new idea, but doing so in an economical way that actually produces materials that people can use has been tricky. Chemists are looking into catalysts that might fit the bill, but it's a pretty hefty bill. [url]


text:
Landmines and Human Security : International Politics and War's Hidden Legacy
AUTHOR: Bryan McDonald, Richard Anthony Matthew, Kenneth R. Rutherford
PUBLISHER: State University of New York Press
Also available at Amazon.com
This book is not guaranteed to come with supple-
mental materials (access codes, CD's, DVDs, etc.)
mental materials (access codes, CD's, DVDs, etc.)
21 Day Unconditional Guarantee
any book, any reason


text:
[Video] V-MODA M-100 Custom Shield Install
The V-MODA one-the-ear and over-the-ear headphones can be customized by ordering a new set of metal shields (faceplates) for the outside of the earcups to express your individual style. These new shields can be ordered for the V-80/M-80, the Crossfade LP2, and the newly announced Crossfade M-100's. For a limited time, users who pre-order the new M-100's before they are released in December can get a custom set of shields for only $10. Existing V-Moda headphone users can get custom shields for $25. Check out my installation video on the M-100's then head on over to V-Moda.com to order a set for yourself!
HeadphonesPro AudioV-modaVideos
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This profile was last updated on 8/26/14 and contains information from public web pages and contributions from the ZoomInfo community.
Debra R. MazzucaWrong Debra R. Mazzuca?
Key Account Manager
Phone: (610) ***-**** HQ PhoneEmail: m***@***.com
Local Address: Los Angeles , California , United States
Air Products and Chemicals , Inc.
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Company Description: Air Products & Chemicals, Inc. serves technology, energy, industrial and healthcare customers globally with a portfolio of products, services and solutions that... more
ProPub_Iteration_NoTests.312[01] SEIDXWK-7


text:
Top Definition
used to immitate another language...any language
i saw this asian nigga down the street and he was all like "coulda mun jarde"...then we be chillin in a french bar and everyone be like "coulda mun jarde"
by schwen February 03, 2004
Type your email address below to get our free Urban Word of the Day every morning!
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Minnie Mouse
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There are a few buzzword-foods that we all know are biggies in the no no department when it comes to keeping our cholesterol in check. These foods are chock full of unhealthy saturated fats, and they can play havoc on your cholesterol levels – your bad cholesterol levels, that is, if you consume them too much.
One of the biggest offenders, if consumed in more than moderation, is eggs. Of course, the egg white is purely nutritious and has plenty of health benefits because it is nothing but pure, fat free protein, and extremely low in calories as well. However, the egg yolk, which itself has some great nutrients and vitamins in it, can be bad when you consume too many of them. This means that you should either eliminate or reduce the size of the yolk if you are consuming eggs, when you scramble or prepare them, and limit your consumption of this part of the egg if you can.
Another food that's bad for your cholesterol regulation is bacon. Not only is bacon high in saturated, cholesterol unfriendly fat, but it is also incredibly salty, which can add to hypertension (high blood pressure), and increase water weight gain, put more pressure on the heart. It's a good thing that trans fats are being reduced or banned in many foods or many locales also, because trans fats were a major offender in the battle against bad cholesterol.
Trans fats are hydrogenated, which means they go through a chemical process that allows them to have a longer shelf life, and also makes them a little tastier, however eating trans fats really has no health benefits whatsoever, and in fact has so many detrimental effects to cholesterol levels and contributing to obesity that it has been banned in many cases by local governments in cities, and also has been banned in several foods by the makers voluntarily, like McDonald's, Burger King, Wendy's and other fast food joints.


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Most incandescent lamps operate by heating a special filament inside a glass bulb filled that is usually filled with an inert gas. The descriptive name "incandescence" comes from the surface physics governed by the material used as a filament. Early lamps used steel and more modern lamps use Tungsten, but for most materials used today most of the energy released by the lamp is in the form of heat and not light. This is why incandescent lamps are so inefficient. Incandescent lamps have a low efficacy (10-17 lumens per watt) compared with other lighting options and a short average operating life (750–2,500 hours).
The three most common types of incandescent lamps are:
- Standard incandescent lamps, which are no longer manufactured but are still in use
- Energy-saving Incandescents (or halogen)
- Reflector lamps
Visit Energy Saver to learn more about the types of incandescent lamps that may be found in homes, as well as efficiency standards that affect the types of lighting you will find in stores.


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A free man after years in prison, Carlito Brigante intends to give up his criminal ways, but it's not long before the ex-con is sucked back into the New York City underworld. Reconnecting with his dancer girlfriend, Gail, Carlito gets entangled in the shady dealings of his friend Dave Kleinfeld, who also serves as his lawyer. When Carlito and Kleinfeld run afoul of shifty gangster Benny Blanco, it sets them on a dangerous path.
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text:
Re: Should I close a credit card I just opened?
HiLine wrote:
BOFA at least takes the highest daily balance (may be up to the minute even) so there's no need to even let that balance report if you're worried about a near-term application.
This is interesting. You're saying that BofA reports to the credit bureaus the highest daily (or minute-ly) balance as the highest balance? I've learned something new today!
Anyway, closing a no-annual fee credit card from a prime lender is almost always a bad idea.
Yeah, I found that out explicitly over the past year; some lenders will only report the high balance if it's an end of statement balance; however, BOFA is not one of them. I had a near maxxed card which I was going to let report before I got the heeby jeebies and simply paid the balance... turns out that the top balance of the month (may not have included pending charges though) was what wound up in the high balance line for my BOFA credit card, rather than the statement balance.
Actually did this twice over the last year and was identical behavior each time. I don't know if it's a daily sweep though or if it's just instant in time highest balance, for most people it doesn't matter too much I suspect unless you're trying to goose the high balance near the end of a reporting cycle and you have a near-term application coming up. Personally I do this on my cards almost as soon as I actually get them and I don't apply for much anyway so it's never been an issue for me.
Current Score: EQ 04 700, TU 04 731, EX 98 725 (05/24/15)
Goal Score: 700 on EQ 04 (01/01/16)
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Good morning, friends!
One of my readers reached out to me about doing a guest post. Since I'm not too active on the blog these days, I loved the idea, and thought you would too.
Holiday Recipes with a Twist
During this festive time of year, it's not just Santa who watches his waistline expand. Between Christmas cookies, gingerbread houses, office potlucks and ugly sweater parties, there is no shortage of delicious, calorie-laden temptations. Indulging in a few holiday treats is a must; indulging for a couple months could be mildly hazardous. In order to help keep the circle of life in balance, consider working these waist-friendly recipes in with your traditional table-fare.
1. Paleo "Knock –You-Right-Off-Your-Feet" Sweet Potato Brownies
This delicious recipe (found here) is courtesy of The Healthy Foodie. She has managed to make the brownie even better than it was by adding mouthwatering melody of sweet potato puree, apple sauce, date paste and an avocado! At a mere 189 calories (including the icing!) this could be the new go-to brownie recipe of the year!
2. Slow Cooker Garlic Sweet Potato Mash
Any recipe that uses a slow cooker gets automatic bonus points, especially during the holidays when oven space is in high demand. This mashed potato recipe (found here) has only 151 calories and 1 gram of sugar per serving. 1% milk and light sour cream help make this dish a bit kinder on the waist. You won't even miss the cream in these yummy yams!
3. Vegan Stuffing
Stuffing is a staple at many holiday tables, and with good reason. The savory combination of flavors trapped inside each nugget of bread is enough to make most guests weak in the knees! This year, consider subbing your usual recipe for this tasteful ensemble (found here). Cranberries, carrots, and some fresh sage help take this vegan dish over the top.
4. Coconut Almond Cranberry Chicken
The flavors in this recipe (found here) make the perfect addition to any fall or winter table. The tart cranberries and sweet coconut milk create a tender, flavorful chicken dish. At only 252 calories per serving, this chicken masterpiece would pair perfectly over stuffing or a dinner roll. If you're looking for a new entrée this season, consider mixing it up with this recipe!
5. Eggnog Holiday Smoothie
Seasonal beverages may be one of the greatest parts of this time of year. From coffee to cocktails, flavorful holiday spirit abounds! To avoid drinking a few meals worth of calories, try a healthy twist on eggnog (found here). Greek yogurt and a banana help substantiate the beverage and hold the calorie count steady at 256/serving.
This year, do yourself a favor and serve your guests a healthy mix of naughty and nice dishes. Life is all about balance, so indulge (smartly) this season and your health (and your jeans!) will thank you.
Kimber is embracing the holiday season with open arms and an abundance of ugly sweaters. When she's not writing, you can find her building a gingerbread house or watching The Year Without A Santa Claus.


text:
Thibault Imbert (Sep 21, 2011)
Write fast GPU accelerated applications without using low-level Stage3D APIs.
Christian Cantrell (Sep 21, 2011)
Learn about the major new features in AIR 3, including API additions and support for iOS, Android, and Adobe AIR for TV.
Oliver Goldman (Sep 21, 2011)
Learn about the new captive runtime and custom installer options for desktop and mobile apps.
Adobe (Sep 21, 2011)
Extend the AIR runtime to achieve deeper integration with target devices and incorporate legacy native code in your apps.
Adobe (Sep 21, 2011)
Learn how minor changes in the 10.3.183 update improve security without affecting SWF content, and stay informed about future product updates.
Tom Green, Joseph Labrecque (Sep 21, 2011)
Stream media files using RTMP with the video-on-demand service and develop a simple video application using ActionScript.
Mixamo, Flare3D (Sep 21, 2011)
Take a character, automatically generate a rig, apply animations, and bring it into Flash with a few clicks.
Renaun Erickson (Sep 21, 2011)
Learn how Renaun Erickson developed the multi-screen mobile application for viewing the Caltrain schedules.
Renaun Erickson (Sep 21, 2011)
The Caltrain Times app is a train schedule viewer for the Caltrain commuter rail system in California.
Jacob Surber (Sep 21, 2011)
Get a high-level preview of what's coming in the new release.
Adobe (Sep 21, 2011)
Use this native extension in your AIR apps for iOS to get information for each network interface from ActionScript.
Adobe (Sep 21, 2011)
Use this native extensions to access the vibration feature of an Android or iOS mobile device from ActionScript.
Adobe (Sep 21, 2011)
Use this native extension to access the gyroscope data of an Android or iOS mobile device from ActionScript.
Artem Pecheny, Yuri Maltsev (Sep 21, 2011)
Animate 3D graphics with Alternativa3D.
Adobe (Sep 20, 2011)
Learn how Stage 3D will change the way developers use 3D on the web. (2:40)
Paul Trani (Sep 20, 2011)
Learn a few tricks for optimizing SWF file sizes by using the Text Container Manager. (4:32)
Ariel Nehmad, Jose Luis Ressia (Sep 18, 2011)
Use the provided assets to learn how to build a 3D sample project in Flash.
Ross Przybylski (Sep 16, 2011)
Hero Mages is a turn-based fantasy game built with Flash that combines strategic card play with battlefield tactics and supports cross-platform multiplayer on web browsers, Android, and iOS.
Paul Trani (Sep 13, 2011)
See how easy it is to manipulate entire sets of layers with a new feature in Flash Professional CS5.5. (2:42)
Tom Green, Joseph Labrecque (Sep 12, 2011)
Walk through the steps of a basic installation of the server for development purposes.
Jesse Freeman (Sep 12, 2011)
Learn how to configure and submit projects for iOS, Android, and PlayBook platforms.
Holly Schinsky (Sep 12, 2011)
Learn how to style the ActionBar and tabs of Flex mobile applications using CSS.
Kevin Towes (Sep 12, 2011)
Learn about the new features in Flash Media Server 4.5, including same-source video delivery to iOS and Flash Player, improved HTTP Dynamic Streaming, and more.
Jack Freudenheim, Benjamin P. Lyons (Sep 11, 2011)
Learn how to create a database table, bind the schema to a form, and store the returned data.
Carl Cargill (Sep 06, 2011)
Take a look at web standards from a tool maker's perspective.


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Cordon Bleu Stromboli
Total Time
Prep 35 mins
Cook 25 mins
This is an easy-to-make dinner or lunch sandwich that looks and tastes great. It is from Quick Cooking magazine and is credited to Diane Schuelke.
Ingredients Nutrition
- 1 lb loaf frozen bread dough, thawed
- 2 tablespoons butter (optional) or 2 tablespoons margarine, softened
- 8 ounces thinly sliced deli ham
- 1 cup shredded swiss cheese
- 5 ounces thinly sliced roasted deli chicken
- On a lightly floured surface, roll dough into a 10-in X 8-in rectangle; spread with butter.
- Top with ham, cheese and chicken.
- Roll up jelly-roll style.
- Starting with a long side; pinch seam to seal and tuck ends under.
- Place seam side down on a greased baking sheet.
- Cover and let rise for 20 minute.
- Bake at 375 for 25-30 minutes or until golden brown.
- Refrigerate leftovers.
Most Helpful
This was the recipe that my husband picked for our weekly dinner menu and it will definitely be made again! The only change that I made to the recipe was that I added mild banana peppers. The hardest part of making this recipe was getting the dough to roll out into the 10x8 square. We also made another stromboli with turkey and cheddar but the Cordon Blue boli was leaps and bounds more delicious than the turkey boli. I took the advice of other reviewers and I baked both bolis on a metal cookie sheet at 400 for 30 minutes and I had no problems with the inside being doughy. This fed our family of 5 (including a toddler) and we had about a quarter boli of each kind leftover. Thank you for posting.
HA! That's my mom! (Diane) It was funny when we saw this in the cookbook as we are MUCh more simple than that!! We simply use bread dough, left over chicken, ham, and what ever cheese is on hand. Then we simply bring the sides together and seal the ends then bake. It's a GREAT way to use up leftovers!!
This was excellent! I used frozen pizza dough instead and it worked beautiful. I think next time I will serve it with marinara sauce?


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"5 Star (Remix)" ft. Nicki Minaj, Trina and Gucci Mane)
The follow up single to Live from the Kitchen, the "5 Star (Remix)" kept it extra turnt up with an even balance between male and female rappers. Gotti's clever ode to the opposite sex wouldn't be complete without a well-constructed verses from Trina and Queen Nicki.
(Photo: RCA Records)
The Night's Big Winners10/14/2014
Cypher Revealed Remy Ma10/10/2014
10 Influential Marks Doug E. Fresh Left On Hip Hop10/02/2014
The Show: Brandy09/23/2014
Lyte Always Shines09/22/2014
Queen Latifah's 8 Best Stage Moments09/22/2014
Yo-Yo's Best Stage Moments09/22/2014
The Show: Young Thug09/19/2014
Cypher Revealed David Banner09/19/2014
Seat Check: Where Your Favorite MCs Are Sitting09/19/2014
The Warm Up: Go Inside Rehearsals09/19/2014
The Doug E. Fresh I Am Hip Hop Playlist09/19/2014
The Show: Jay Electronica09/19/2014
The Show: Rich Homie Quan09/19/2014
The Show: DJ Premier09/19/2014
The Show: YG09/19/2014
Common's 7 Best Stage Moments09/18/2014
Bobby Shmurda's Top Shmoney Dance-Worthy Performances09/18/2014
Rae Sremmurd Got What It Takes To Rock The Mic09/17/2014
Migos' 6 Most Trill Performances09/17/2014
The Show: Ty Dolla $ign09/17/2014
T.I. Swags Out At Every Show09/17/2014
The Show: Vince Staples09/16/2014
Meet The MCs Set Ignite The Cypher09/16/2014
Birdman Stays Fly on Stage09/15/2014
Emilio Rojas and Crew Bring the Buzz to The Cypher09/12/2014
Battle and Cypher Ready09/12/2014
Mustard on the Beat...and Stage09/10/2014
We Got MCs: Find Out Who's Performing09/09/2014
Cypher Revealed | Kevin Gates09/08/2014
Cypher Revealed Live Cypher09/08/2014
Cypher Revealed: Lil Mama09/08/2014
Cypher Revealed Taylor Gang09/08/2014
Uncle Snoop Returns: 10 Reasons Our Host Is The King of Cool09/04/2014
Browse The Full List Of Nominees08/28/2014
The Cypher Revealed - TDE10/15/2013


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Timberwolves (14-49, 5-25 road) vs. Thunder (35-29, 21-9 home)
TV: FS Oklahoma
Stream: Click here
Radio: WWLS The Sports Animal (98.1 FM, 640 AM, 1300 AM The Buzz Tulsa)
Time: 7:00 CT
Offensive Rating: Thunder – 106.4 (12th), T-Wolves – 102.6 (26th)
Defensive Rating: Thunder – 103.9 (11th), T-Wolves – 110.8 (30th)
The Thunder is once again looking up at the 8th seed. This time, it's the New Orleans Pelicans that hold that spot by .5 a game with 18 games to play. Tonight the Thunder hosts the last place Minnesota Timberwolves and will look to pull even with the Pelicans with a win.
The Wolves will be without Anthony Bennett, Robbie Hummel and Shabazz Muhammad due to injury, while center Nikola Pekovic is a game time decision. Keep Reading…


text:
love it ^^
what a cutie! I love her!
omg, what's with the knife?! D: I just hope she's not gonna use it on the birdie
anyway, really awesome artwork as always!
anyway, really awesome artwork as always!
"i was a little girl..alone in my little world" ...beautiful..the colors....your pictures are amazing :]
Beautiful. Love the expression on her face. Great job as always!
Aww, how cute! xD
this is soo pretty
she is verry prettyy
she came out just superb! Love it!
omgosh...I have been in LOVE with your art and style for a long time now, since like..before I ever thought about joining DA. I'm a fan! You are so talented! BTW, she is soo pretty and cute! XD
I adore her! Ahh!
I love these kinds of characters. <3
i have a pet bird too.... I LOVE THIS. O.o
Very cute and deadly.
I love the colours!
Adorable work!
thats really pritty ^_^
I love how it looks all cute, then there is that little knife on her supporters Hair hair is so nice
This is so incredibly cute!!!!!!!!!!!! Love the colours and just makes me want a cute little parrot... Awesome kinkei...
Oh your art work is so beautiful. I wish I had the money to buy a commission.
Fun colors (: lovely x
So cute~!
She's so adorable!
This is soo cute!
I lahk the birdie!
Such lovely colors! And no one will suspect a cutie like her of being an assassin
i just love your style!
Cute! I love the dress print
I really love the colors!
Her facial expression is very cute~!
And I love her outfit.
And I love her outfit.
how much 4 a commish?
Oh my gosh! This came out amazing!
I love the colors, and the pose! And the birdie!
Ah! It's all perfect!!
Thank you so so much!
I love the colors, and the pose! And the birdie!
Ah! It's all perfect!!
Thank you so so much!
Awww I love the parrot!
Wait why is there a knife on her leg? D:
Wait why is there a knife on her leg? D:
Lovely as always! I love the colors, <3
Aww, this is so cute! I love the hair!


text:
Originally posted by wildcat
Why didnt we ever listen to our good old George Washington whos the one who told us to stay out of debt
Originally posted by Qwazzy
Ok, here's what I found. Plz say something else than "this isn't true."
This money isn't gonna be payed back.
buddy, you've been sleeping a LONG time!!!!
try this one out............
rumsfeld says "pentagon missing 2 (two) trillion dollars"


text: We went with a 50% off voucher from El Tenedor. The deal was one tapa and one main course for half price. The tapas were huge, more like generous starters (I had Argentinian patatas bravas) and the main courses were also massive (vacio). None of us could finish our main course which was a shame... read more


text:
Bright yellow. Melon, yellow apple, white flowers and buttered toast on the fragrant nose and in the mouth. Round and fleshy on entry, then more taut in the mid-palate. Finishes stony and long, with good vivacity and a lingering floral note.
**Editors' Choice** A rich wine with crisp apple and grapefruit flavors as well as a touch of sweetness, Lanson's non-vintage Brut always has fine acidity with just a hint of toast indicating some bottle age. It's bottled ready to drink.
Flinty, smoky and rooty, this builds freshness from underneath the reductive notes, yielding floral lemon scents. It's round and balanced. For aperitifs.
Subtle flavors of pear and white cherry are underscored by smoke, bread dough and spice notes in this balanced bubbly, which ends with a citrusy finish. Disgorged March 2013. Drink now.
This house is making a big comeback. A big toasty nose leads to a convincing, rich palate impression and a clean, refreshing finish. The wine is made from 50% Pinot Noir, 35% Chardonnay and 15% Meunier and vinified without malolactic fermentation. The fruit comes from all Grand and Premier Cru areas within Champagne and it tastes like it! (Gary Westby, K&L Champagne buyer)
The item you have chosen is in stock, and has inventory in at least one of our retail stores. Below is the current quantity on hand information for this product within our database. It is never more than five minutes old. Additionally, our shopping cart looks at real time inventory so when you add an item to you cart we will do an immediate check of available inventory and alert you if there are any issues.
Baron Fuente "Grande Reserve" Brut Champagne
Franck Bonville Grand Cru Brut Blanc de Blancs Champagne
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How To Replace The Valve Cover Gasket On A Toyota Camry
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adrian wellman: were abouts did u put the ultra grey
jerry Dishman: Great video! really helps out,thanks!
SYNCPHAN1OM: oil is leaking behind on the top of trans next to sensor and on top of exhaust idk if it could be the oil pan or valve cover or cam plug
coltcomphbar: You should have resealed the cam plug.
(on the older Camry models they had a distributor o-ring. They use the same head on the 97-01 models with electronic ignition so you need to reseal the plug that goes where the distributor used to be)
KATHLEEN DUVALL: I have a 1999 Camry, Oil was sopping # 4 plug. used a 10 mm wrench, purchased a 30mm socket, purchased new plugs, wires, Gasket set. went back for Sealant, then went back for PCV valve. Then went back for a O ring Grommet for the valve. Took longer to go shopping and down time, and I am no mechanic, BUT, Shooter250 Props to You, Camry running like a Swiss Watch now. Thanks......
Nat Funk: I have a 93, and it is the same.
Nat Funk: That would be the valve guide seals. replacing the seals would also involve changing the valve cover gasket because you need to remove the valve cover to get started on the valve guide seal repair.
masskhamisi4: No white smoke means your burning...not leaking
Abraham venegas: Should this be replaced if your car shoots out white smoke when you start it? thanks
Terri McDaniel: Thanks so much, couldn't have done it without with out this video!!
Caleb Warren: great video, learned a bunch!
shooter250: I would guess yes if you want to keep it from leaking again.
shooter250: yes
terri steele: on the 2.2 ltr toyota camry vavle cover replacement is it mandatory to add the 4 dots of permatex on each side of engine?
Rene Romero: wpuld this be the same for a 93 camry?
fukthbullshit: thnxs my camry is a 95 and was leaking oil from their
Amir Cash: any difference between this and replacing a blown head gasket?
Stanley Hill: JUST DID THIS ON A 1996....MAN WAS IT FUN, PCV HOSES SNAPPED LIKE TWIGS...GOTTA REPLACE THEM.....DID THE SPARK PLUGS AND TUBE SEALS AS WELL :)
ns671fr: haynes manual is pretty tight at 33ft lbs, i still saw an old toyota shop book and it was at 13, but revisions to it show now 17. Just thought Id throw it out there.
ns671fr: Actually its 17ft lbs. I've owned a few 5sfe's. Previously it was 13 but the revised is 17.
How to Replace the Valve Cover Gasket on a Toyota Camry 5 out of 5