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Home / Graduate / Molecular Biophysics Training Grant /
Recent Trainees
Meredith Brenner
While on the Molecular Biophysics Training Grant, I began my research developing a new multiphoton FRET microscopy technique using ultrafast laser pulse shaping. This technique will permit selective excitation of multiple fluorophores in a biological system, as well as provide quantitative information about the concentrations of donor and acceptor molecules and their temporal dynamics in cellular processes. While on the training grant, I implemented the ultrafast pulse shaping and used that to achieve selective excitation of fluorophores in solution. I have subsequently applied this pulse shaping technique to live-cell imaging, obtaining fluorescence images of live Cos-7 cells expressing fluorescent proteins. I have achieved selective excitation of the fluorophores in the cells and observed FRET in the cells. In addition, I have learned how to perform fluorescence lifetime measurements and used those results to determine the FRET efficiency of my system.
I have developed MATLAB code to design pulse shapes as well as perform image processing and analysis, and have also extended existing FRET theory to quantify my results. I am currently performing calculations on my live-cell image data. In addition, I am exploring other applications of ultrafast pulse shaping to live-cell imaging, such as selective excitation in different systems and imaging systems containing multiple spectrally-overlapped fluorophores. I have been trained in the use of ultrafast lasers, basic wet lab techniques, the use of a two-photon laser scanning microscope, and tissue culture techniques to maintain, transfect, and image living cells. I have also taken classes introducing me to a variety of nonlinear optical and biophysical techniques, their theoretical bases, and recent advances in the field.
"Pulse-shaping-based Two-photon FRET Microscopy," Meredith H. Brenner, Dawen Cai, Samuel W. Straight, Joel A. Swanson, and Jennifer P. Ogilvie. Proceedings of Ultrafast Phenomena 2012.
"Pulse Shaping Multiphoton FRET Microscopy." Meredith H. Brenner, Dawen Cai, Sarah R. Nichols, Samuel W. Straight, Adam D. Hoppe, Joel A. Swanson, and Jennifer P. Ogilvie. Proceedings of SPIE 8226, 82260R, 2012.
Taeyjuana Curry
I began my research in the Kopelman lab working to refine techniques used for external calibration of organically modified silane (ORMOSIL) nanoparticles utilized for intracellular electric field sensing in cancer cells. In the last few years, my research has primarily been focused on nanoparticle-mediated photothermal therapy (PTT) of cancer cells. My research has successfully elucidated that covalently linked Coomassie Blue dye polyacrylamide (PAA) nanoparticles can successfully be used for effective PTT of cancer cells, enabling another step in a multimodal approach to cancer detection and treatment using the same nanoplatform that was used for optical and photoacoustic imaging. Finally, my most recent research includes the efficient delivery of femtosecond laser ablation generated gold nanoparticles into cancer cells nuclei, aimed at enabling more effective PTT, as well as other forms of therapies. Upon completion of my Ph.D program in Physics, I would like to expand my research to include the use of nanoplatforms in the detection, imaging, and treatment of malignant cell lines.
Antonuk LE, El-Mohri Y, Du H, Behravan M, Curry T, Zhao Q, Koniczek M, Street R, and Lu JP: Exploration of the Potential Performance of Polycrystalline Silicon-based Active Matrix Flat Panel Imagers Incorporating Active Pixel Sensor Architectures. SPIE Medical Imaging 2008 - Physics of Medical Imaging, San Diego, CA, February 16-21, 2008. (Abstract and oral presentation.)
Behravan M, Antonuk LE, El-Mohri Y, Zhao Q, Yeakey M, Martelli C, McDonald J, Curry T and Koniczek M: Noise Characterization of Polycrystalline Silicon Thin Film Transistors for X-ray Imagers Based on Active Pixel Sensor
Architectures. Materials Research Society Spring 2008 Meeting: Symposium A – Amorphous and Polycrystalline Thin-Film Silicon Science and Technology, San Francisco, CA, March 24 - 28, 2008. (Abstract and oral presentation.)
Taeyjuana Curry, Tamir Epstein, Ron Smith, & Raoul Kopelman. Photothermal Therapy of Cancer Cells mediated by Blue Hydrogel Nanoparticles. Under review.
Taeyjuana Curry, Wei Qian, & Raoul Kopelman. Surface Optimization of Gold Nanoparticles for Controlled Delivery Into Cancer Cell Nuclei. Submitted.
Scott Horowitz
At the outset of my studies, little was known of the functions and importance of CH O hydrogen bonding in biology. To address the dearth of information on this subject, I originated a collaboration between the Al-Hashimi and Trievel labs to bring multiple biophysical and biochemical techniques to bear, as well as developing techniques specifically to examine CH O hydrogen bonding. I focused my research on the role of CH O hydrogen bonds in AdoMet-dependent methylation, as it is of prime importance to many cellular functions. Previous studies had suggested that the catalytic methyl group might form CH O hydrogen bonds within the methyltransferase active site. Using multiple techniques, I was able to show that not only are CH O hydrogen bonds present in all seven structural classes of AdoMet-dependent methyltransferases, but that they are required for both cofactor binding and catalysis. Additionally, using NMR spectroscopy and X-ray crystallography, I have experimentally determined the atomic-scale structure and motions of the catalytic methyl group and its hydrogen bonding partners, and found that these interactions aid in catalysis both by stabilizing the positively charged transition state, as well as restricting methyl group motion. Of note, as these interactions are the only known conserved element in the many different structural classes of AdoMet-dependent methyltransferases, we believe that these interactions were a critical element in methyltransferase convergent evolution, and are fundamental to the methyl transfer reaction.
Rafiee P, Theriot ME, Nelson VM, Heidemann J, Kanaa Y, Horowitz SA, Rogaczewski A, Johnson CP, Ali I, Shaker R, Binion DG. Human Esophageal Microvascular Endothelial Cells Respond to Acidic pH Stress by PI3K/AKT and p38 MAPK-regulated Induction of Hsp70 and Hsp27 Am J Physiol Cell Physiol, 291(5) C931-945 (2006)
Horowitz S, Binion DG, Nelson VM, Kanaa Y, Javadi P, Lazarova Z, Andrekopoulos C, Kalyanaraman B, Otterson MF, Rafiee P. Increased Arginase Activity and Endothelial Dysfunction in Human Inflammatory Bowel Disease Am. J. Physiol Gastrointest Liver Physiol, 292(5)G1323-G1336 (2007)
Krishnan S*, Horowitz S*, Trievel RC. Structure and Function of Histone H3 Lysine 9 Methyltransferases and Demethylases Chembiochem, 12(2):254-263 (2011)
Horowitz S, Yesselman JD, Al-Hashimi HM, Trievel RC. Direct Evidence for Methyl Group Coordination by Carbon-Oxygen Hydrogen Bonds in the Lysine Methyltransferase SET7/9, J. Biol. Chem, 286(21):18658-63 (2011)
Horowitz S, Dirk LMA, Yesselman JD, Nimtz J, Del Rizzo PA, Mehl RA, Houtz RL, Al-Hashimi HM, Trievel RC. Methyl CH O Hydrogen Bonds Orchestrate AdoMet-Dependent Methylation Under review
Frank AT, Horowitz S, Andricioaei I, Al-Hashimi HM. Use of 1H NMR Chemical Shifts in Determining RNA Structure and Dynamics In preparation
Horowitz S, Trievel RC. Carbon-Oxygen Hydrogen Bonds in Biology, J. Biol. Chem, invited review article, submitted
Yesselman JD*, Horowitz S*, Trievel RC, Brooks CL. Systematic Analysis of Methyl CH O Hydrogen Bonding in Proteins In preparation
Horowitz S*, Fick RJ*, Trievel RC. Mechanistic Aspects of AdoMet-Dependent Methylation invited review article, to be published May 2013
*denotes equal contribution
Alex Johnson-Buck
The past decade has seen a rapid expansion of interest in the construction of nanoscale materials and devices from DNA owing to its predictable self-assembly properties and the ease with which it can be functionalized to position and interact with other functional materials. The maturation of this field requires a detailed understanding of the impact of design parameters on assembly yield and performance. To this end, I am using single-particle fluorescence techniques to study the kinetic and chemical properties of DNA nanomaterials.
In particular, I have developed two techniques: nanoscale chemical fingerprinting and single-origami kinetic assays. The first technique combines single-particle localization with reversible binding of fluorescent probes to specific features of a target structure – in this case, a DNA origami tile with patterned surface features – to generate a quantitative nanoscale map of the interactions between the structure and components in solution. Using a two-color approach, I can distinguish different patterns of surface features and monitor enzymatic modifications of the surface patterns. I have also discovered stable spatial distributions of binding, or "fingerprints" of interactions, that are unique to each DNA tile and not predictable based on the design. This technique empowers us to characterize subtle, but important chemical properties of single nanostructures with unprecedented spatial and temporal detail.
The second technique, single-origami kinetic assays, involves monitoring the cumulative fluorescence or FRET (Förster resonance energy transfer) signal from individual nanoscale DNA origami tiles as they bind fluorescently labeled components from solution.. Using this technique, I find that interactions between adjacent binding sites have a significant impact on DNA hybridization kinetics and overall yield. These findings have important implications for the growing number of applications in which components are assembled on DNA scaffolds with controlled stoichiometry and spacing.
Johnson-Buck, Alexander E.; Blanco, Mario R.; Walter, Nils G. (2012). Single-molecule fluorescence resonance energy transfer. In Encyclopedia of Biophysics. 1 (G. Roberts, Ed.), Springer, in press.
Blanco, Mario R.; Johnson-Buck, Alexander E.; Walter, Nils G. (2012). Hidden Markov modelling. In Encyclopedia of Biophysics. 1 (G. Roberts, Ed.), Springer, in press.
Michelotti, Nicole; Johnson-Buck, Alexander; Manzo, Anthony J.; Walter, Nils G. (2011) Beyond DNA origami: the unfolding prospects of nucleic acid nanotechnology. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology. DOI: 10.1002/wnan.170.
Marek, Matt S.; Johnson-Buck, Alexander E.; Walter, Nils G. (2011). The shape-shifting quasispecies of RNA: One sequence, many functional folds. Physical Chemistry Chemical Physics. 13, p. 11524-11537.
Johnson-Buck, Alexander E.; McDowell, Sarah E.; Walter, Nils G. (2011). Metal ions: Supporting actors in the playbook of small ribozymes. In Metal Ions Life Sci. 9 (A. Sigel, H. Sigel, R.K.O. Sigel, Eds.), The Royal Society of Chemistry, Cambridge, UK, in press.
Lund, Kyle A.; Manzo, Anthony J.; Dabby, Nadine; Michelotti, Nicole; Johnson-Buck, Alexander; Nangreave, Jeanette; Taylor, Steven; Pei, Renjun; Stojanovic, Milan N.; Walter, Nils G.; Winfree, Erik; Yan, Hao. (2010). Molecular robots guided by prescriptive landscapes. Nature 465, 206-210.
Michelotti, Nicole, de Silva, Chamaree, Johnson-Buck, Alexander E., Manzo, Anthony J., Walter, Nils G. (2010). A bird's eye view: tracking slow nanometer-scale movements of single molecular nano-assemblies. Methods in Enzymology 475, 121-148.
Johnson-Buck, Alex; Kim, Gwangseong; Wang, Shouyan; Hah, Hoe Jin; Kopelman, Raoul. Fabrication, Characterization, and Spectral Properties of Indigo Blue Nanocrystals. (2009). Mol. Cryst. Liq. Cryst. 501, 138-144.
Josh Jasensky
Vibrational spectroscopy has proven to be a unique tool in determining chemical behaviors of a variety of biological systems. These techniques offer intrinsic selectivity, as these techniques are able to probe different chemical signatures. Such methods have been particularly applicable for use in non-invasive imaging and surface characterization.
The health, growth, and preservation of human oocytes have been the targets of many advanced screening methods which determine viability as well as likelihood of eventual fertilization. The most accurate and widely used assays for determining oocyte health are based on morphological scoring, which are prone to the most error. Part of my thesis research has focused on developing coherent anti-Stokes Raman scattering (CARS) microscopy into a powerful non-invasive analytical tool in screening for cryopreservation candidate selection and providing a method to permit modification cryoprotectant exposure regimens to improve oocyte cryotolerance. To date, I have successfully developed a method to calculate total lipid content which has been shown to directly correlate with certain developmental stages in oocyte maturation. My eventual goal is to modify oocytes with established cryoprotectant exposure protocols and determine whether a chemical difference can be determined which then can be used to refine previous protocols.
Another facet of my thesis research also focuses on the biological interactions of peptides at an abiotic surface. Although peptides are often associated with an aqueous environment, it is important to be able to build a surface which contains active proteins in the absence of bulk water. This has applications in textiles, medical devices as well as the military where environments are not necessarily conducive to certain proteins. Currently I am using sum frequency generation (SFG) vibrational spectroscopy to characterize surfaces with chemically immobilized anti-microbial peptides (AMPs). SFG is a surface-sensitive technique which can be used to monitor protein secondary structure. The next goal of this research is to modify this abiotic surface in order to facilitate these peptides to keep their proper secondary structure and AMP activity.
Khamaladze A, Matz RS, Epstein T, Jasensky J, Banaszak-Holl MM, and Chen Z. Cell volume changes during apoptosis monitored in real time using digital holographic microscopy J. Struct. Biol. 2012, 178(3):270-278.
Ye SJ, Li HC, Wei F, Jasensky J, Boughton AP, Yang P, and Chen Z. Observing a Model Ion Channel Gating Action in Model Cell Membranes in Real Time in Situ: Membrane Potential Change Induced Alamethicin Orientation Change J. Am. Chem. Soc. 2012, 14(134):6237-6243.
Liu Y, Jasensky J, and Chen Z. Molecular interactions of proteins and peptides at interfaces studied by sum frequency generation vibrational spectroscopy Langmuir 2012, 28(4):2113-2121.
Khamaladze A, Jasensky J, Zhang C, Han XF, Ding J, Seeley E, Liu XR, Smith GD, and Chen Z. Hyperspectral microscopic imaging by multiplex coherent anti-Stokes Raman scattering (CARS) Proc. SPIE 2011, 8158, 815805.
Zhang C, Wang J, Khmaladze A, Liu Y, Ding B, Jasensky J, and Chen Z. Examining Surface and Bulk Structures Using Combined Nonlinear Vibrational Spectroscopies Opt. Letts. 2011, 36(12):2272-2274.
Stephanie Le Clair
While on the training grant, Sum Frequency Generation (SFG) was utilized to monitor the real-time membrane interaction of truncated forms of human and rat islet amyloid polypeptide (IAPP). IAPP is the main component in amyloid plaques found on the pancreatic islets of Langerhans in type II diabetes patients. Truncated human IAPP(1-19) has been shown to be cytotoxic and does not form fibers. Whereas truncated rat IAPP(1-19) only differs from human IAPP(1-19) at residue 18, it is significantly less cytotoxic. The goal of the research was to ascertain the difference in cytotoxicity between these two peptides. Using SFG, the amide I band of each peptide was deconvoluted to determine the secondary structure and membrane orientation. This was done for membranes of diverse compositions and under several buffer conditions. FTIR-ATR was also used to cross-validate the orientations obtained with SFG. Differences in the SFG limits of detection were shown when comparing rat IAPP(1-19) and human IAPP(1-19).
When the training grant ended, I continued research under the NSF graduate research fellowship and changed projects. The goal of my current project is to characterize the structure and dynamics of the full-length cytochrome b5-cytochrome P450 (cyt b5-cyt P450) complex in a membrane environment. These two proteins have an important role in the metabolism of a variety of endogenous and exogenous compounds but little is known regarding how the full-length proteins interact with one another. Solution NMR was used to probe the complex formation between these two proteins in isotropic bicelles, both in the presence and absence of a cyt P450 substrate. The interaction interface of cyt b5 for cyt P450 was mapped out and this information, as well as double mutant cycle analysis data, were used to deduce the cyt b5-cyt P450 complex structure with HADDOCK. Through a series of NMR experiments, we have also begun to address the role of electrostatic interactions in the encounter complex formation between cyt b5 and cyt P450 and have determined the key hydrophobic "hot spot" residues of cyt b5 which are essential in binding to cyt P450. We are also unraveling the kind of role that cyt P450 ligands play in the interaction of cyt P450 with cyt b5. Specific regions of cyt b5 and cyt P450 are also being studied to understand their role in complex formation: the linker region of cyt b5 and the transmembrane domain of cyt P450.
Le Clair, S. V.; Zhang, M.; Im, S.-C.; Waskell, L.; Ramamoorthy A. "Anatomy of the cyt b5 interface in its stereospecific complex and encounter complexes with cytochrome P450." In preparation.
Ahuja, S.; Jahr, N.; Im, S.-C., Vivekanandan, S.; Popovych, N.; Xu, J.; Soong, R.; Le Clair, S. V.; Nanga, R. P.; Yamamoto, K.; Bridges, A.; Waskell, L.; and Ramamoorthy, A. "A dynamic structure of the membrane-bound cytochrome b5-cytochrome P450 complex from NMR and mutagenesis data." Submitted.
Nguyen, K.; Le Clair, S. V.; Ye, S.; and Chen, Z. "Orientation Determination of Protein Helical Secondary Structure Using Linear and Nonlinear Vibrational Spectroscopy." J. Phys. Chem. B. 2009, 113, 12169-12180.
Nguyen, K.; Le Clair, S. V.; Ye, S.; and Chen, Z. "Molecular Interaction between Magainin 2 and Model Membranes in Situ." J. Phys. Chem. B. 2009, 113, 12358-12363.
Ye, S.; Nguyen, K. T.; Le Clair, S. V.; and Chen, Z. "In Situ Molecular Level Studies on Membrane Related Peptides and Proteins in Real Time Using Sum Frequency Generation Vibrational Spectroscopy." J. Struct. Biol. 2009, 168, 61-77.
Le Clair, S. V.; Nguyen, K.; and Chen, Z. "Sum Frequency Generation Studies on Bioadhesion: Elucidating the Molecular Structure of Proteins at Interfaces." J. Adhesion. 2009, 85, 484-511.
Esmonde-White, K. A.; Le Clair, S. V.; Roessler, B. J.; Morris, M. D. "Effect of Conformation and Drop Properties on Surface-Enhanced Raman Spectroscopy of Dried Biopolymer Drops." Appl. Spectrosc. 2008, 62, 503-511.
Stephen Norris
The cell is an extremely complex system that is highly regulated both spatially and temporally. Subcellular cargoes including organelles, endosomes, lysosomes, and mRNA granules are often directed to specific locations in the cell in a time-dependent fashion. This targeted intracellular trafficking is usually accomplished by molecular motor proteins such as kinesin and dynein, which drive cargo motion along cytoskeletal tracks. Collections of multiple motors working together are thought to be required to generate sufficient force and distance for transport of a large cargo through the dense cytoplasm. Over the past two decades, highly precise single-molecule experiments have shown how a single kinesin or dynein motor generates force in vitro. Considerations of multiple-motor cargo transport in live cells are muddled by the complexity of the cell, and multiple-motor in vitro experiments are highly artificial and do not allow ideal experimental precision.
We propose to reconcile these two types of experiments by developing a synthetic biology method to create well-characterized multiple motor complexes. We use a protein-based method to specifically attach known numbers of motor proteins to scaffolds of known length and flexibility. We express these custom-designed constructs in mammalian COS-7 cells and assemble multiple-motor complexes in live cells. The motility properties of these complexes can then be studied in live cells or in vitro via TIRF (Total Internal Reflection Fluorescence) microscopy. This method allows us to investigate many outstanding questions in the motor field with a high degree of accuracy and precision. Although these synthetic biology methods are being applied to molecular motors here, one could conceivably use these tools to create more general multi-protein complexes in live cells where genetic fusion does not suffice.
(In preparation – Biophysical Journal): J.A. Cribb, P.A. Vasquez, P. Moore, S. Norris, S. Shah, M.G. Forest, and R. Superfine. Nonlinear signatures of entangled polymer solutions in active microbead rheology.
Matt Stone
I am exploring how B lymphocyte's antigen receptor (BCR) signaling utilizes lipid organization to regulate signaling. Although lipid phase separation in model membranes has been shown extensively, there is little direct evidence that lipid organization exists and has a function in live cells. However, many theories explaining how BCR antigen binding is communicated across the plasma membrane assert that lipid organization either drives or regulates this process by controlling the partitioning of membrane proteins to the BCR proximal. I aim to clarify the role of lipid-mediated heterogeneity in BCR signal regulation through the use of two-color super-resolution microscopy. I have applied highly accurate image registration to our lab's pre-existing super-resolution imaging techniques to allow for accurate two-color single molecule microcopy. This has facilitated observations of lipid reorganization which are dependent upon BCR antigen binding. Branched and saturated lipids respond to BCR clustering differently, which is consistent with lipid phase behavior seen in model membranes. An understanding of how lipid organization affects signaling through by the BCR may allow for novel drugs targeting lipids to treat B cell based autoimmune diseases and cancers. In addition, I am characterizing the fluorescence of indocarbocyanine dyes for use in quantitative two-color super-resolution and developing new analysis methods based on correlation functions, both of which will allow for highly accurate measurements of spatio-temporal correlation between separately labeled biomolecules. I hope to share these developments through publications and conferences in order to facilitate further research in these areas by other research groups.
Chen, Ran, Tatsiana A Ratnikova, Matthew B Stone, Sijie Lin, Mercy Lard, George Huang, JoAn S Hudson, and Pu Chun Ke. 2010. "Differential uptake of carbon nanoparticles by plant and Mammalian cells." Small (Weinheim an Der Bergstrasse, Germany) 6 (5) (March 8): 612-617.


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Publications (6)8.04 Total impact
Conference Paper: Development of a new method for in vivo objectivation and quantification of cutaneous spots by image analysis[Show abstract] [Hide abstract]
ABSTRACT: Drawing on profilometry, we developed a new method which would enable the quantification, by image analysis, of certain properties linked to the perception of pigmented spots on the skin. Based on digital images of spots, an initial visual assessment was carried out according to three quite distinct classes: a class of "hardly visible" spots, a class of "visible" spots and a class of "very visible" spots. These same different profilometric parameters were calculated and correlated to the visual classification. Globally, the results showed very strong coherence between the assessment and the instrumental approach. Lastly, an efficacy study of a depigmenting product showed the relevance and sensitivity of our new methodology
Article: Whitening efficacy of plant extracts including orchid extracts on Japanese female skin with melasma and lentigo senilis[Show abstract] [Hide abstract]
ABSTRACT: The purpose of this study was to assess the in vivo efficacy of a cosmetic formulation containing plant extracts including orchid extracts, compared to 3% vitamin C derivative formulated with the same excipient, in Japanese female adult volunteers with melasma and/or lentigo senilis. The ethics committee of Osaka National Hospital approved the protocol of the study. Before recruitment, selection and inclusion of a volunteer in this study, signed informed consent was obtained from each volunteer after she was given clear and precise information on the study, enabling her to appreciate the aim of the study and the consequences of her consent. Forty-eight female volunteers aged 30-60 years applied the plant extracts and vitamin C derivative to one side of the face. After repeated application for 8 weeks, efficacy was evaluated clinically by colorimetric measurements and subjectively using a questionnaire. After 8 weeks of treatment, both the clinical evaluations by a dermatologist and the questionnaire surveys by volunteers indicated that the cosmetic formulation containing plant extracts was significantly effective in improving the size, brightness, color intensity, clarity, visibility and global appearance of the pigmented spots, and also the luminosity complexion and skin clarity of the face. The good agreement between the results of clinical evaluations and those of questionnaire surveys showed that the orchid-rich plant extracts possess efficacy similar to vitamin C derivative in whitening the skin as well as melasma and lentigo senilis on the face of Japanese women.
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ABSTRACT: [corrected] The signs of aging may originate from natural processes or from exposure to the sun, wind, or other environmental factors. To evaluate the anti-aging effects of potential agents researchers must first identify and be able to quantify epidermal markers that change with aging. This paper summarizes the results of studies conducted to evaluate the transcriptional effects of an Aframomum angustifolium seed extract and Malva Sylvestris extract, and the antiaging efficacy of a skin care product containing the Aframomum angustifolium seed extract. The transcriptional effect of an Aframomum angustifolium seed extract on normal human keratinocytes (NHKs) and normal human fibroblasts (NHF) was evaluated in vitro with the use of a low-density DNA array technology. The Malva Sylvestris extract was studied with a commercial DNA macroarray and by a real-time quantitative reverse transcriptase-polymerase chain reaction. The in vitro anti-aging activities of the Malva sylvestris extract were compared with those of all-trans retinoic acid (RA), a well-established topical therapy for photodamage and wrinkles. The genes studied were known to be modified by RA. The anti-aging efficacy of a facial skin care product containing Aframomum angustifolium seed extract was evaluated in a single-center study using image processing analysis and in a 2-center study by evaluation of the photographs by the investigator, independent evaluators, and subjects. In general, the Aframomum angustifolium seed extract strongly modified the gene expression profiles of NHKs and weakly modified the gene expression profiles of NHFs. After incubation with Aframomum angustifolium seed extract, the expressions of 3 antioxidant genes (metallothionein 1, metallothionein 2, and thioredoxin) were increased in NHKs, while expressions of 1 antioxidant gene (glutathione peroxidase) was increased in NHFs. Concerning the Malva sylvestris extract, a cDNA macro-array technology experiment with the reconstructed human epidermis model showed that some genes modulated by treatment with the Malva sylvestris extract are also regulated by RA treatment indicating a similar activity at the mRNA level. In the single-center study, a facial skin care product containing the Aframomum angustifolium seed extract significantly improved the homogeneity of the skin. The areas of the detected objects (skin imperfections) decreased significantly on each studied area of the face and the variance decreased significantly over the entire face. In the 2-center study, 28% percent of the subjects reported a greater than 50% overall global improvement in their skin by the end of the study compared to 11% of the subjects after 4 weeks of treatment. Seventy-six percent of subjects said they would purchase the cream. The authors developed a low-density DNA chip method that permitted the study of the transcriptional effect of Malva Sylvestris extract and of Aframomum angustrifolium seed extract. The gene expression profiles obtained demonstrate the anti-aging properties of these compounds. An in vivo single-center study, performed and analyzed with an assay based on image processing analysis, demonstrated the antiwrinkle activity of a formulation containing the Aframomum angustifolium seed extract. The data obtained in the 2-center study suggests that the cosmeceutical containing Aframomum angustifolium seed extract produces a global rejuvenation effect in terms of redness, pigmentation, and fine lines similar to that noted utilizing an intense pulse light source.
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ABSTRACT: Cell longevity is linked to sirtuins (silent information regulators), which belong to a family of enzymes implicated in gene silencing, apoptosis, fatty acid metabolism, and regulation of cellular life spans of organisms. Sirtuins are associated with genes that coordinate and optimize the functions of cells as cells struggle to survive in a stressful environment, as it is the case for skin cells. This study focuses on 1) yeast Kluyveromyces biopetides in stimulating the expression of sirtuin in human cutaneous cells and 2) the benefit for the skin of an active skin care product containing yeast Kluyveromyces biopetides. Silent mating type information regulation 2 homolog 1 (SIRT1) was investigated by immunostaining, Westem blotting, and cytometry on normal human skin cells in culture and on healthy skin samples ex vivo. SIRT7 are mammalian versions of the yeast SIR2 gene. Cellular integrity and aging was followed by comet assays measuring DNA fragmentation and beta galactosidase activity (a marker of senescence). The test product was yeast Kluyveromyces biopeptides. Thirty-three female subjects aged 37 to 64 years (mean 51.6 years) enrolled in the study. Subjects applied a formulation enriched in 1% of the yeast biopeptides SIRT1 activator once daily to the face and neck for 4 weeks. Dermatologists used a graded scale (1-9) to score fine lines and wrinkles, hydration, pigment color intensity, complexion radiance, skin density, firmness, complexion homogeneity, and texture of the skin before and after the first application and again after 4 weeks of use. A Pixel Skin method, based on an analysis of the gray-level variance and surface of imperfections (age-related parameters) from numerical pictures of the faces, was used to objectively measure the skin care efficacy. The yeast Kluyveromyces biopeptides 1) significantly increased SIRT1 expression in normal human dermal skin fibroblasts in vitro (+172%) and in epidermal cells of healthy human skin ex vivo and 2) decreased cell senescence and DNA fragmentation induced by ultraviolet-B (UVB) stress. At the end of the study, facial improvements could be seen on fine lines and wrinkles, hydration, pigmented spot color intensity, complexion radiance, firmness, complexion homogeneity, and texture. Improvement in hydration was significant immediately after the first application. Skin-pixel measurement and analysis show a significant reduction of the gray variance linked to pixel heterogeneity (-4.2%) and a significant reduction of the surface of skin imperfections (-30.4%). All the indicators from clinical evaluation to the objective measurements of the skin show a significant improvement of the aged skin. These results demonstrate the efficacy of the yeast Kluyveromyces biopeptides in activating SIRT 1 of human skin cells, improving their DNA resistance and senescence, and of a formulation enriched in this ingredient in treating multiple skin aging signs.
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ABSTRACT: Skin without significant dyschromia is an aesthetic goal of people worldwide. Current options for lightening skin could have significant drawbacks. The antisense strategy may be a viable alternative. The reactions in melanogenesis are catalyzed mainly by tyrosinase, tyrosinase-related protein 1 (TRP-1), and TRP-2. Activation of tyrosinase is associated with phosphorylation by protein kinase C-betaI (PKC-betaI) and formation of a complex between phosphorylated tyrosinase and TRP-1. The aim of this study was to use 2 antisense oligonucleotides to modulate the synthesis of the tyrosinase/TRP-1 complex, PKC-beta, or both by interacting with the targeted mRNA, thus whitening skin by interfering with melanogenesis at the translational level. METHODS/STUDY DESIGN: In the in vitro study, the effect of the antisense oligonucleotides was evaluated by measuring the rate at which dihydroxyphenylalanine (DOPA) oxidase transforms L-DOPA to DOPAchrome in the pathway for melanin biosynthesis. A reduction in the reaction rate compared to the controls corresponded to a decrease in the enzyme activity and, consequently, to a reduction of the formation of melanin pigments. To evaluate the in vivo lightening effect of the antisense oligonucleotides, 30 Asian women volunteers with pigmented spots on both hands applied the test product twice daily for 8 weeks. The test product was applied to 2 marked-off areas of the hand: a pigmented spot (to evaluate the effect of the test product on the color of the spot) and a nonpigmented spot area (to evaluate the effect of the test product on normal skin pigmentation). The lightening effect was evaluated by comparing chromametric and mexametric parameters before treatment, after 4 weeks, and after 8 weeks. In vitro DOPA-oxidase activity was inhibited by 13% in melanocytes treated with the antisense sequence for PKC-BI alone, by 16% with the antisense sequence for TRP-1 alone, and by 36% with the association of 2 sequences. The inhibiting effect with both sequences required the specific sequences with nonreversed polarities. In vivo clinical results showed statistically significant whitening in both pigmented spots and nonpigmented spots when the test product was applied twice daily for 8 weeks by up to 30 Asian women. The association of TRP-1 and PKC-betaI antisense molecules significantly increased the inhibition of tyrosinase activity on human melanocytes. Antisense oligonucleotides are a new generation of active cosmetic ingredients that offer unprecedented specificity, biological stability, and safety in lightening skin. This is the first report of positive results in a cosmetic based on the use of these new active agents.
Article: In vivo skin antioxidant effect of a new combination based on a specific Vitis vinifera shoot extract and a biotechnological extract[Show abstract] [Hide abstract]
ABSTRACT: BACKGROUND AND OBJECTIVES: Ultraviolet (UV) light produces reactive oxygen species (ROS) in skin, which accelerate aging by damaging DNA, proteins, lipids, and other cellular constituents. The aims of this study were to 1) evaluate the antioxidant properties of a Vitis vinifera shoot extract on cultured normal human keratinocytes, 2) compare the in vivo antioxidant of this extract in combination with a biotechnological extract (Ronacare Hydroine), and 3) evaluate the efficacy on photoaging skin of a serum based on a combination (Vitis vinifera shoot extract in hydroglycolic solution, or Sarmentine, and Ronacare Hydroine) after a 4-week application, and to quantify the additional improvement given by applying a cream with the serum. METHODS/STUDY DESIGN: An in vitro study was conducted to evaluate the antioxidant properties of Vitis vinifera shoot extract added to cultured normal human keratinocytes. A fluorescent probe was used to quantify cytoplasmic endogenous species formed in response to oxidative stress induced by H2O2. The antioxidant activity of Vitis vinifera shoot extract was compared to that of a solvent control and 2 positive controls, vitamin E and vitamin C. In the first in vivo study, 2 test products were included in a comparative, randomized, single-blind trial in which 27 subjects acted as their own (untreated) controls. Products were applied 4 times to randomized areas of the inner surface of the forearm for one day. The following day, treated and untreated (control) areas of stratum corneum were sampled for fluorimetric analysis. A decrease in fluorescence compared with untreated control reflected a decrease in the level of ROS, in which case the product had a scavenging effect. The 2 products contained a combination of Sarmentine and Ronacare Hydroine, whose antioxidant properties were under investigation. Other products were known antioxidants. In the second in vivo study, 60 female subjects applied either serum or serum plus cream twice daily for 28 days for clinical evaluation. Overall improvement was rated on a quartile scale (0%-25%, 26%-50%, 51%-75%, 76%-100%) and changes in firmness, radiant glow, evenness, smoothness, wrinkles, fine lines, hydration, texture, and softness were rated on a negative to positive scale (-5=worse to +5=greatly improved). RESULTS AND CONCLUSIONS: Vitis vinifera shoot extract appears to have significantly stronger in vitro antioxidant capacity than vitamin C or vitamin E. In the same vehicle (placebo emulsion), ascorbic acid (0.5%), Sarmentine (1%), and the Sarmentine (1%) plus Ronacare Hydroine (1%) combination had a significant in vivo antioxidant effect versus a nontreated area. The combination Sarmentine (1%) plus Ronacare Hydroine (1%) showed a higher efficacy than Sarmentine alone. The dermatologic evaluation showed that a 4-week twice-daily application of a serum containing the combination improved the main clinical signs of photoaged skin. The addition of the cream with the serum appears to enhance the serum-induced improvement of most of the skin characteristics.
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Imagine a stack of numbered cards with one on top. Discard the top,
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predict the last card?
Given the nets of 4 cubes with the faces coloured in 4 colours, build a tower so that on each vertical wall no colour is repeated, that is all 4 colours appear.
Find the point whose sum of distances from the vertices (corners)
of a given triangle is a minimum.
If all the faces of a tetrahedron have the same perimeter then show that they are all congruent.
We're excited about this new program for drawing beautiful mathematical designs. Can you work out how we made our first few pictures and, even better, share your most elegant solutions with us?
A Hamiltonian circuit is a continuous path in a graph that passes through each of the vertices exactly once and returns to the start.
How many Hamiltonian circuits can you find in these graphs?
The reader is invited to investigate changes (or permutations) in the ringing of church bells, illustrated by braid diagrams showing the order in which the bells are rung.
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A standard die has the numbers 1, 2 and 3 are opposite 6, 5 and 4 respectively so that opposite faces add to 7? If you make standard dice by writing 1, 2, 3, 4, 5, 6 on blank cubes you will find. . . .
This article for teachers discusses examples of problems in which
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to think deeply about the context and extend their ability to. . . .
Lyndon Baker describes how the Mobius strip and Euler's law can
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A game for 2 people. Take turns joining two dots, until your opponent is unable to move.
This is a simple version of an ancient game played all over the world. It is also called Mancala. What tactics will increase your chances of winning?
The aim of the game is to slide the green square from the top right
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Euler discussed whether or not it was possible to stroll around Koenigsberg crossing each of its seven bridges exactly once. Experiment with different numbers of islands and bridges.
A bus route has a total duration of 40 minutes. Every 10 minutes,
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meet on its way from one end to the other end?
A huge wheel is rolling past your window. What do you see?
Can you find a rule which relates triangular numbers to square numbers?
A visualisation problem in which you search for vectors which sum
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Can you discover whether this is a fair game?
Can you cross each of the seven bridges that join the north and south of the river to the two islands, once and once only, without retracing your steps?
A game for 2 players. Can be played online. One player has 1 red
counter, the other has 4 blue. The red counter needs to reach the
other side, and the blue needs to trap the red.
Here is a solitaire type environment for you to experiment with. Which targets can you reach?
A rectangular field has two posts with a ring on top of each post.
There are two quarrelsome goats and plenty of ropes which you can
tie to their collars. How can you secure them so they can't. . . .
Glarsynost lives on a planet whose shape is that of a perfect
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Charlie and Alison have been drawing patterns on coordinate grids. Can you picture where the patterns lead?
The whole set of tiles is used to make a square. This has a green and blue border. There are no green or blue tiles anywhere in the square except on this border. How many tiles are there in the set?
Use the interactivity to listen to the bells ringing a pattern. Now
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Slide the pieces to move Khun Phaen past all the guards into the position on the right from which he can escape to freedom.
Show that among the interior angles of a convex polygon there
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Two angles ABC and PQR are floating in a box so that AB//PQ and BC//QR. Prove that the two angles are equal.
The triangle OMN has vertices on the axes with whole number co-ordinates. How many points with whole number coordinates are there on the hypotenuse MN?
Mathematics is the study of patterns. Studying pattern is an
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Start with a large square, join the midpoints of its sides, you'll see four right angled triangles. Remove these triangles, a second square is left. Repeat the operation. What happens?
These are pictures of the sea defences at New Brighton. Can you
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ABC is an equilateral triangle and P is a point in the interior of
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must be less than 10 cm.
Given a 2 by 2 by 2 skeletal cube with one route `down' the cube.
How many routes are there from A to B?
To avoid losing think of another very well known game where the
patterns of play are similar.
This is an interactive net of a Rubik's cube. Twists of the 3D cube become mixes of the squares on the 2D net. Have a play and see how many scrambles you can undo!
Players take it in turns to choose a dot on the grid. The winner is the first to have four dots that can be joined to form a square.
Imagine you are suspending a cube from one vertex (corner) and
allowing it to hang freely. Now imagine you are lowering it into
water until it is exactly half submerged. What shape does the
surface. . . .
If you can copy a network without lifting your pen off the paper and without drawing any line twice, then it is traversable.
Decide which of these diagrams are traversable.
Imagine starting with one yellow cube and covering it all over with
a single layer of red cubes, and then covering that cube with a
layer of blue cubes. How many red and blue cubes would you need?
Every day at noon a boat leaves Le Havre for New York while another
boat leaves New York for Le Havre. The ocean crossing takes seven
days. How many boats will each boat cross during their journey?
How could Penny, Tom and Matthew work out how many chocolates there
are in different sized boxes?
In the game of Noughts and Crosses there are 8 distinct winning
lines. How many distinct winning lines are there in a game played
on a 3 by 3 by 3 board, with 27 cells?
Four rods, two of length a and two of length b, are linked to form
a kite. The linkage is moveable so that the angles change. What is
the maximum area of the kite?
Two boats travel up and down a lake. Can you picture where they
will cross if you know how fast each boat is travelling?
Use the interactivity to play two of the bells in a pattern. How do
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Anne completes a circuit around a circular track in 40 seconds.
Brenda runs in the opposite direction and meets Anne every 15
seconds. How long does it take Brenda to run around the track?


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Truck driver Kevin Roper in court
Wednesday, June 11, 2014 3:27 PM
Truck driver Kevin Roper at his first appearance at the Middlesex County Courthouse on June 11, 2014. Roper is charged in the accident that injured comedian Tracy Morgan. At left is defense attorney David Glassman. The Star-Ledger file photo
Related articles
- Tracy Morgan crash: Wal-Mart truck driver pleads not guilty to charges he killed 1, injured 4 June 11, 2014, 3:27 PM
- Tracy Morgan crash: NTSB says truck driver was speeding and had worked 13 hours straight June 19, 2014, 11:11 AM
- Tracy Morgan's lawyers accuse Wal-Mart of stalling crash lawsuit December 02, 2014, 2:39 PM
- Lawyer: Tracy Morgan's attorneys threatening truck driver's right to a fair trial December 11, 2014, 5:33 PM
- Judge refuses to delay Tracy Morgan lawsuit, report says December 13, 2014, 12:14 PM
- Judge won't delay Tracy Morgan civil lawsuit over Wal-Mart crash February 04, 2015, 4:09 PM


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Monday, March 28, 2011 11:35 p.m.
By Dave Johnson
CSNWashington.comSalt Lake City---This is not your fathers or even Jerry Sloans Utah Jazz anymore, but that didnt reduce the challenge. Playing the second of back-to-back games in a building where they have not won in five years, the Wizards stunned the Jazz with a 100-95 overtime victory.With 28 points, including 24 in the first half, John Wall led the Wizards to their second road win of the season. Jordan Crawford added 25 points and JaVale McGee completed his fourth consecutive double-double with 11 points and 17 rebounds.The win not only prevented a 0-5 road trip, but also eliminated any chance of the Wizards matching the 199091 Sacramento Kings (1-40) for fewest road wins in a season. For the Jazz, who were led in scoring by C.J. Miles with 17 points, it was their sixth straight loss.It was almost the cruelest of endings for the Wizards who led for most of the game. Sparked by rookies Derrick Favors (Georgia Tech), Gordon Hayward (Butler), and Jeremy Evans (Western Kentucky) , the Jazz went on a 14-3 run to erase a 10-point deficit and take an 84-83 lead with 45 seconds left in regulation.The Wizards were reeling. Short-handed again with Nick Young and Trevor Booker still injured, the Wizards only managed 12 points in the fourth quarter. Crawford scored 8 of those points including a 12-foot jumper from the baseline with 4.4 seconds on the clock to force overtime.Crawford then jump-started the Wizards in overtime with consecutive steals that led to four points and an 89-85 lead. Maurice Evans gave the Wizards breathing room with a three-pointer from the corner to extend their lead to 92-85 with 3:18 to go in the extra session.It was an offensive rebound by Othyus Jeffers that led to Evans critical three-pointer. On his second ten-day contract with the Wizards, Jeffers provided the necessary jolt of energy to spur a tired team. In the fourth quarter and overtime, Jeffers had 5 offensive rebounds. In the final 35 seconds of overtime Wall went 4 of 6 from the free throw line to limit a late surge by the Jazz. It was Walls first points since the first half when he had little trouble going at Earl Watson and the Jazz man-to-man defense.We controlled tempo for most of the game, said Wizards head coach Flip Saunders. John (Wall) destroyed them when they went man-to-man. They went to zone and we lost some of our aggressiveness. They got themselves back in the game. But we were able to have some of our big guys make some big plays.The Jazz are still in playoff contention, but they are clearly struggling in the aftermath of Sloans retirement as head coach and the trade of Deron Williams. Devin Harris missed a fourth consecutive game with a hamstring injury, while Andrei Kirilenko and Mehmet Okur are also sidelined by injuries.Al Jefferson had 15 points and 16 rebounds, but did not play in the fourth quarter or overtime. Jazz head coach Tryone Corbin elected to stick with the younger lineup that triggered their fourth quarter rally.The Wizards only bad game on their western journey was the trips opener in Portland. They pushed the Clippers to overtime and against the Nuggets and Warriors just could not find a way to score enough points.
This time, a result to match the resilience.


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afc69Budding Enthusiast
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- Ford Model Focus 1.6 Zetec S
Thanks again for the replies guys. However, Will - I don't think that facia panel would fit my car. The photo I attached doesn't quite show the top edge of my existing panel, but it goes up to include the hazard warning switch. The link you sent seems to have a square top edge, whereas mine would need to be curved in the middle of the top edge to go round the switch.
That's very kind of you Will. As you can see from the photo in the origonal post, my current stereo is the more rounded one. So, given that my current stereo if dual DIN, if I go for a new single DIN stereo, do you need a new facia panel plus a facia plate to cover the missing DIN, or do you retain the facia panel and just need the facia plate? Are the wiring connections identical, so it's just plug and play? Are all car stereo wiring connections generic?
So, would something like this work in Ford Focus:- http://www.amazon.co.uk/gp/product/B0033C27GM/ref=s9_simh_gw_p23_d0_g23_i1?pf_rd_m=A3P5ROKL5A1OLE&pf_rd_s=center-2&pf_rd_r=1TWJYNDDX49775S6HETE&pf_rd_t=101&pf_rd_p=467128533&pf_rd_i=468294 Where abouts would you get the facia plate from, the fit the Focus?
Thanks John. What kind of aftermarket headunit is compatible with the 2009 Focus?
Bluetooth And Ipod Connectivity Options
afc69 posted a topic in Ford Focus ClubI am looking to bluetooth my mobile to my 2009 Focus, with the attached stereo, and also connect my iPod. I have the aux connection, but I want the full control of the iPod from the stereo. I bought a Connects2 unit, which I was advised would work with my iPod and stereo, but on installation, found it didn't work, and then the company I bought it from realised it wasn't compatible with my stereo (although it did charge the iPod) So, does anybody have any suggestions for me, or do I have to buy a whole new stereo to enable me to do these things?
Ipod Integration Kit For 2009 Focus 6000Cd Stereo
afc69 posted a topic in Ford Focus ClubAS the title says, I'm looking to get an iPod Classic integration kit for my Focus 6000CD. I already use ther 3.5mm audio output, but want a better system whereby I can control the iPod from the head unit, along with the capacity to charge my iPod and have track listing on my head unit. See a lot a stuff about Connects2, are they the best to go with?
iPod USB retrofit for Ford Focus 2009
afc69 posted a topic in Audio & ElectronicsI can play my iPod through the 2.5mm headphone socket in the glove box, but I can't control it from the stereo head unit. I assume if you have the USB socket in the centre console, you can then do so? I have a 2009 Focus, 6000 head unit. Is it possible to get the USB socket fitted retrospectively?
Thanks, silverfox. Okay. The manual descibes how you can play MP3s burnt onto a CD-R/RW, but doesn't specify which model. So we are saying that the 6000CD doesn't support MP3s played on CDs? Is the glovebox aux input an factory fitted option, as I don't appear to have it? I assume this is to hook up an iPod?
BUMP Come on, somebody must know?
Playing MP3s on Ford Focus 6000CD
afc69 posted a topic in Ford Focus ClubI assumed you could play MP3s on a CD in the 6000CD unit, but I get a CD error message. Does this unit actually take Cds with MP3s on it?
Rear parking sensors
afc69 posted a topic in Ford Focus ClubI notice that rear parking sensors are available as a factory fitted option on the newer Focus's, but is it something that can be fitted to an 09 plate as a retro fit?
Panther Black
afc69 replied to afc69's topic in Ford Focus ClubSorry to be thick here but you don't get Panther Black in non metallic, do you?
Panther Black
afc69 posted a topic in Ford Focus ClubIn other words, if you see a Ford car advertised as Panther Black, is it definetly metallic?
Ford Focus Zetec 1.6
afc69 posted a topic in Ford Focus ClubHave put down a deposit on a 5 month old Zetec 1.6, but am wandering if I should hold out for a 1.8. In your opinion guys, is the 1600 a tad underpowered?


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Pronunciation: /lo͞oˈandə/
Words that rhyme with LuandaAmanda, Aranda, Baganda, Banda, brander, candour (US candor), coriander, dander, expander, gander, germander, goosander, jacaranda, Leander, Lysander, meander, memoranda, Menander, Miranda, oleander, panda, pander, pasanda, philander, propaganda, Rwanda, sander, Skanda, stander, Uganda, understander, Vanda, veranda, withstander, zander
Definition of Luanda in:
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Disclaimer for broken windows?
Would this hold up? And if not, how come? Thought I saw on here a while back, but can not find now, a disclaimer a guy had his clients sign saying that unless you allow him to inspect the yard, for a fee(like $10 per visit, which no one would agree to pay), before mowing he is not responsible for negligence from items throw by the mower or trimmers. "Negligence" is the key, because as professional, we should be operating in a safe, responsible manner so we should not be responsible for something somewhat out of our control.
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Luna is Angry by alfalunaWatch
©2013-2016 alfaluna
-I bet you know who is She angry with.... >.> . One of the first drawings I made of Luna this 2013 after my disappearance from the comic.
- Supongo que saben con quien esa enojada...>.> . Uno de los primeros dibujos que hice de Luna en todo el 2013 luego de mi ausencia en el cómic.
- Supongo que saben con quien esa enojada...>.> . Uno de los primeros dibujos que hice de Luna en todo el 2013 luego de mi ausencia en el cómic.
OK lets some clear... I am not the one that pushed her into the water!!!!
luego de mi ausencia en el comic
...por que?!
It's an EXCELLENT comic!!!
Una excelente imagen de Luna
adoro ese rostro, te quedo perfecta
"Thought you could keep me down on hiatus for long, did you? Big mistake buddy..." Seriously, she even looks as if she was crawling out a hole or something ^^
Glad to have ya back again Leo!
Glad to have ya back again Leo!
Man I love that comic! I was like "What?!" at the very last page, this deviation and your AL comic is amazing ^^
She looks great!
She can be as angry as she wants if she looks that good.
nice and dangerous... and somewhat smexy
I need to learn that word
I just hope its the right translation
I read many werwolf-stories from Kelley Armstrong and Patti Briggs
And from the forum I heard that smexy is a mix between smart and sexy. Smexy.
Perfect for Luna... if you ask me.
Niiice. Are you gonna add some colors?
Looks great
Your improving so much it's amazing!


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*Spoilers* TNA One Night Only: Victory Road & Knockouts Knockdown PPV Results
by Amish Patel - May 11, 2014
– TNA taped their One Night Only: Victory Road pay-per-view in Orlando on Saturday. Below are spoilers, thanks to Eric:
* The winner of the event will receive a future World Heavyweight Title shot and a check. The winners of the matches will compete in a battle royal for the main event.
* Bram defeated Davey Richards.
* Austin Aries defeated Kenny King.
* James Storm and Kazarian defeated Knux and The Freak.
* Bobby Lashley defeated Samoa Joe with help from Kenny King.
* Abyss defeated Mr. Anderson
* BroMans defeated Bully Ray and Rockstar Spud when Spud turned on Bully.
* Gunner defeated Magnus
* Ethan Carter III defeated Sanada.
* Samuel Shaw defeated Crazy Steve.
* Eddie Edwards defeated Zema Ion and Tigre Uno.
* Gunner last eliminated James Storm to win the battle royal, a future title shot and a check. Participants were Gunner, Storm, Kazarian, Edwards, Shaw, EC3, Abyss, Lashley, Aries, Bram, Jessie Godderz and Robbie E.
– TNA is taped their One Night Only: Knockouts Knockdown pay-per-view in Orlando on Saturday. Below are spoilers, thanks to Eric:
* Gail Kim defeated Veda Scott.
* Angelina Love defeated Scarlett Bordeaux.
* Madison Rayne defeated Jessicka Havok.
* Reby Sky defeated Velvet Sky after Angelina Love accidentally sprayed Velvet with hairspray.
* Taryn Terrell defeated Karlee Perez.
* Mia Yim defeated Brittany.
* Brooke Tessmacher defeated Deonna.
* Marti Belle defeated ODB with help from her new boyfriend, Rockstar Spud.
* ODB squashed Rockstar Spud.
* Tessmacher, Yim, Taryn, Reby, Angelina, Madison, Marti and Gail went into a Gauntlet Match for the main event. It came down to Madison and Angelina. Madison got the win to be the 2014 Knockouts Queen.
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|Daniel Bryan Retires From Pro Wrestling
|WWE Star Suspended After Incident With Vince McMahon, Length Of His Suspension Reduced
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|Daniel Bryan Reportedly Gives His Notice To WWE And Was Denied
|CM Punk Undergoes Successful Surgery


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Board Candidates on Bad Decisions — The Democratic candidates for Arlington County Board on Wednesday were asked to name a recent County Board decision they disagreed with. James Lander said he disagreed with the decision to cancel the streetcar. Andrew Schneider said "citizens were owed more of a process" on the streetcar decision. Katie Cristol cited the decision not to fund an extra firefighter to staff certain fire equipment. Christian Dorsey cited the decision to delay building an elementary school next to Thomas Jefferson Middle School. Bruce Wiljanen said he wasn't happy with the development plan for Rosslyn Highlands Park. Peter Fallon disagreed with the redevelopment of the Bergmann's dry cleaning site on Lee Highway. [InsideNova]
ACFCU Named 'Credit Union of the Year' — Arlington Community Federal Credit Union, an ARLnow.com advertiser, has been named "Federal Credit Union of the Year" by the Arlington-based National Association of Federal Credit Unions. ACFCU was bestowed the honor for credit unions with assets of $250 million or less.
Babe Ruth Baseball to Celebrate Anniversary — Arlington Babe Ruth (ABR) Baseball will celebrate its 30th anniversary on May 17. Reportedly the longest-running youth baseball program in the county, ABR will mark the occasion with a "Family Fun Day" for baseball families from 2:00-5:00 p.m. in Barcroft Park.
Peter Chang Fast Casual Restaurant in Arlington — Chef Peter Chang, who has a large following in Northern Virginia, is in lease negotiations for the Oriental Gourmet space at 2503 N. Harrison Street. Chang hopes to open Peter Chang Wok, envisioned as a fast casual Chinese restaurant. Chang only plans to make "a few cosmetic changes" to Oriental Gourmet, which is still open, after taking over the lease. [Washington Post]
Cherrydale Plan Passes — Cherrydale has a new Neighborhood Conservation plan. The plan, approved by the County Board on Tuesday, calls for protecting trees, ensuring sidewalks are wide enough for strollers and those with disabilities, timely utility maintenance, more daycare opportunities and infrastructure for residents to age in place. [Arlington County]
Top County Staff Gets Raise — The Arlington County Board on Tuesday voted to give a 3.4 percent raise to the three county employees it's permitted by law to hire directly: County Manager Barbara Donnellan, County Attorney Stephen MacIsaac and Clerk to the County Board Hope Halleck. The annual salary for Donnellan — who's in charge of the county government and its more than 3,800 employees — will increase to $269,742. [InsideNova]
Abundance of Busted Pipes — This week Arlington County firefighters have responded to a steady stream of calls for busted water pipes in buildings around the county. "Please make sure you know where your water shut off is in case it happens to you," the fire department tweeted. [Twitter]
Abingdon Street House Fire — Firefighters extinguished a small fire in the basement of a home on the 100 block of N. Abingdon Street on Wednesday morning. One person had to flee the home, reportedly while only wearing shorts and a t-shirt, but no injuries were reported. [Twitter]
Court Ruling May Cost Arlington Millions — A ruling by the Virginia Supreme Court on a tax dispute in Arlington County may cost Arlington and other Virginia localities millions of dollars in lost business license tax revenue. The court ruled that companies with offices in multiple states may deduct certain out-of-state earnings from their license tax. [Washington Post]
GW Baseball Blanks Georgetown — In a chilly game at Arlington's Barcroft Park that we previewed Wednesday, the George Washington University baseball team defeated Georgetown in a 3-0 shutout. [GW Sports]
Flickr pool photo by Dennis Dimick
Through multiple snowstorms and historic cold, the George Washington University baseball team has endured, practicing and playing on its home turf in Arlington's Barcroft Park.
Because Tucker Field at Barcroft Park is artificial turf, with the exception of the pitching mound, batter's box and bullpen, the team has been able to practice outdoors most days, and has already played two home games; a win and a loss against the New Jersey Institute of Technology.
Many youth baseball players remember especially cold days as especially painful, with balls hit off the end of the bat resulting in hands stinging with pain for several minutes. But for serious college athletes, the cold is roundly ignored.
"It's all a mental thing," graduate student and fifth-year pitcher Craig LeJeune said in a phone interview today. "We've just got to wear a lot more shirts and undershirts. Once you get out there and warm up, you just go out there and play like it's any other day."
The Colonials' third game is today at 2:30 p.m. against Georgetown University, at Barcroft Park. In between their two season-opening games against NJIT, the Colonials have had three games cancelled and two, against Georgetown and the University of Virginia, postponed.
The cancelled games have not meant that the Colonials have gotten a break. They have had some indoor practices, but most of their work has been outdoors, including when the field is still covered in snow.
"The biggest thing we like to do is keep it high-energy and high-tempo so the cold doesn't really affect us," assistant coach and recruiting coordinator Dave Lorber said. "If you're doing the right things and running an efficient practice, the cold is not something you're even thinking about."
After hosting Georgetown tomorrow, Tucker Field will be the site of a three-game series against Niagara University this weekend. The Colonials start play against Atlantic 10 opponents March 20 with a trio of games against St. Joseph's University in Philadelphia.
LeJeune said this year's goal is to qualify for the A-10 tournament, played at Barcroft Park for the first time May 20-24.
"We want to make that [tournament] and defend the Tuck," he said.
Wardian Featured in Runners World — Prolific ultramarathon runner and Arlington resident Michael Wardian was the subject of a full-page spread in the latest Runners World magazine. Wardian, 40, holds the record for fastest marathon dressed as a superhero, fastest marathon with a stroller and fastest marathon on a treadmill. He was photographed on the Potomac Heritage Trail, where he regularly goes on morning runs with his puppy, Rosie. [PDF]
Arlington Wine Bars Make Top 100 — Arlington wine bars The Curious Grape and Grand Cru have been named to OpenTable's list of the top 100 wine-centric restaurants in America. [OpenTable]
Candidates Question School Costs — School Board candidates Barbara Kanninen and Audrey Clement both said that there are ways to contain costs at Arlington Public Schools. Clement said the school system should consider increasing class sizes, while Kanninen said she was concerned about the cost of technology initiatives. [InsideNova]
GW's Barcroft Park Field to Be Named — George Washington University will name the field its baseball team plays at Barcroft Park after a major donor. The field recently underwent a $3 million renovation. It will be named after Avram "Ave" Tucker, a former GW baseball player and the owner of a financial firm, who is making a $2 million donation to the school. The newly-christened "Tucker Field" will be dedicated in a ceremony Saturday morning. [George Washington University]
Orthopaedic Center to Open in Clarendon — The Orthopaedic Foot and Ankle Center of Falls Church has announced that it has opened a second office, at 1307 N. Highland Street in Clarendon.
Flickr pool photo by Erinn Shirley
Decision Lens Gets $6.5 Million — Arlington-based software company Decision Lens has received $6.5 million in investment. The company has 71 employees and serves 80 customers. [Reuters]
Arlington Youth Baseball Team Wins State Title — The Arlington Senior Babe Ruth 15-Under All Stars have captured a state championship. The team will now compete in the Southeastern Regional Tournament, which starts tomorrow near Richmond. [InsideNova]
(Updated at 1:00 p.m.) The spring sports season is over for all but the elite teams, like Washington-Lee boys soccer and Yorktown boys lacrosse, which are still hoping to raise more banners in their gyms.
The Generals' boys soccer team is in the midst of one of its best seasons ever, sporting an undefeated 16-0 record with three ties. It took home the Liberty Conference 6 championship last week and continues its march to the state tournament at home against Stonewall Jackson tomorrow night.
Washington-Lee girls soccer (10-3) also took home the Conference 6 title, defeating Yorktown before losing in the opening round of Region 6A North action, 4-2, to Centreville last night. The Patriots soccer squads both lost just three times this season, and their years wrapped up in similarly heartbreaking fashion: both squads lost in double-overtime last night in the opening round of the regional tournament, the boys to Centreville and the girls to Robinson Secondary School.
Wakefield's boys soccer team is still playing after turning around what could have been a disappointing season. The Warriors were the runner-ups in the Conference 13 tournament after losing six straight games before the tournament began. They play Leesburg's Tuscarora in Region 5A North action tomorrow night to try to keep their improbable postseason alive.
Yorktown's boys lacrosse team, after losing to state powerhouse Langley in the conference champion, secured a huge win last night in the opening round of the regional tournament, blowing by Oakton 12-5. They'll face South County at a neutral field, Robert E. Lee High School in Springfield, tonight at 7:00 p.m. The Patriots girls lacrosse team — also a Conference 6 runner-up — saw its season come to an end last night at the hands of Robinson, 22-12.
Washington-Lee baseball, despite going just 11-11 in the regular season, managed to make a deep run in its conference tournament and qualify for regional play. Arlington's only remaining baseball team in action will hope to keep its season alive at 3:30 p.m. tomorrow in Chantilly.
Wakefield's softball team is also still alive after a 10-9 season and a 15-1 loss to eventual conference champion Marshall in the Conference 13 semifinals. Their season continues on the road in regional tournament play tomorrow at 6:00 p.m. against Massaponax.
After the jump, the records and results for Wakefield, W-L and Yorktown's major varsity team sports. (more…)
The Arlington County Board is set vote on whether to direct field fees collected on baseball and softball diamonds toward maintaining and enhancing those fields.
Currently, the county collects fees from all leagues that use county fields — for youth, it's $8 per resident and $20 for non-residents. It's $100 per team per season for adults — but there is no dedicated funding stream for the improvement of baseball and softball fields. The fees have, to this point, been used to fund the replacement of soccer and other rectangle fields with artificial turf.
If approved, the Field Fund would be segregated, and fees collected from using diamond fields would be reserved just for those fields. In addition, staff is asking the Board to dedicate $85,000 in seed funding for the field improvements.
Funds will be assigned to projects following recommendations from Parks and Recreation staff and the county's Sports Commission. The County Board is expected to vote on the plan this weekend.
Beauty Pageant in Crystal City — The annual Miss United States beauty pageant is taking place at Synetic Theater in Crystal City today and tomorrow (Saturday). Single women between the ages of 20 and 29 come from 55 states and U.S. territories to compete in the pageant. [Miss United States]
Marymount Launching Baseball Program — Arlington's Marymount University is launching a baseball program, with a team composed largely of freshmen from Northern Virginia high schools. The team will play at Bishop O'Connell High School's baseball field. [Washington Post]
County Still Reviewing Streetcar Finance Options — Arlington County officials are still trying to decide on their preferred funding mechanism for the Columbia Pike streetcar project. The streetcar could be built and in operation as early as 2017. [Sun Gazette]
Yelp Details 'Yuppie' Concentration in Clarendon — The business review site Yelp has published a map that shows the concentration of certain words in reviews. In the D.C. area, the word "yuppie" has the highest concentration in Clarendon. It also shows up to a lesser degree in Ballston, Courthouse and the Columbia Pike town center area. [Yelp]
Flickr pool photo by Ddimick
Yorktown Baseball Sets Record — Yorktown High School's baseball team has finished the regular reason undefeated in the National District. With 14 victories, the Patriots set a National District record for wins in a single season. [Sun Gazette]
Crystal City BRT Named — The Bus Rapid Transit system that will serve Crystal City and Potomac Yard next year will be named "Metro Way" and will feature a blue bus design. The buses will travel from Pentagon City to Alexandria, and will run every 6 minutes during the rush hour. [Greater Greater Washington]
USS Arlington Fundraising $100K Short of Goal — So far, more than $390,000 has been donated or is expected to be pledged for a 9/11 "tribute room" on the USS Arlington. That leaves the ship's commissioning committee more than $100,000 short of its $500,000 goal, however. Fundraising is continuing and committee member Frank O'Leary says he is "hoping to crack $400,000 this month."
A grand opening was held for the newly renovated Barcroft Baseball Field #6 over the weekend.
The field's $3 million renovation was paid for by George Washington University. Under an agreement with Arlington County, the school's baseball team will be able to call the field home for the next 20 years, while the public will still be able to use the renovated facility when it's not otherwise reserved for GW games or practices.
The new artificial turf field was open for GW's baseball season last year, but major renovations — including the 500-seat grandstands, heated press box, concession stands, permanent dugouts, GW Colonials-branded entry plaza and reconfigured parking lot — were still under construction during and after the season.
Among those taking part in a ribbon-cutting and first pitch ceremony on Saturday were Arlington County Board Chair Walter Tejada, GW President Steven Knapp and GW Athletic Director Patrick Nero. GW played Quinnipiac after the grand opening festivities, but lost 4-2.
In addition to serving as a home for the GW baseball team, Field #6 will provide Arlington's recreational youth baseball leagues "with a near major-league experience," the county said on its website. GW and Arlington County officials say the long-awaited renovations will provide a big boost to the school's baseball program and to recreational baseball in Arlington.
"Having played baseball at this university, I can speak firsthand about the incredible strides that have been made from where GW Baseball used to call home to this immaculate facility that ranks among the best in the region and in our conference," GW Head Coach Gregg Ritchie said in a statement. "For all of the ballplayers that have come through GW over the years, Barcroft Park should serve as a nod of thanks for paving the way."
"This is a great day for baseball in Arlington," said Tejada. "Through our very successful partnership with GW, Arlington now has its first turf baseball diamond. I want to thank GW baseball for working with us for nearly two decades in what has become a great partnership with our community. We look forward to enjoying many years of great play on this field by both the Colonials and Arlington baseball leagues."
Girl Scout Cookie Sales Start Tonight — As a reminder, Girl Scout Cookie booth sales start at 3:00 today in Arlington. The first day of booth sales might be a bit soggy, as a wintry mix of snow, sleet, freezing rain and rain is expected to start early this afternoon.
School Boundary Petition Started — A petition asking for various changes to the Arlington Public Schools boundary review process has collected more than 75 signatures. [iPetitions]
AP Scores Edge Up for APS Students — The average Advanced Placement exam score for Arlington Public Schools students edged up from 2.88 in 2011 to 2.89 last year. In 2010, however, the average for APS was 3.08. The national average is 2.83, on a 1 to 5 scale. [Sun Gazette]
DJO, Marymount Sign Baseball Agreement — Bishop O'Connell High School and Marymount University have finalized a joint use agreement for the high school's new baseball field. "The future of Catholic education depends on strong partnerships between our high schools and universities," said outgoing Bishop O'Connell President Kathleen Prebble. [Arlington Catholic Herald]
Arlington Homes For <$500k –A real estate blog has found 15 "starter homes" under $500,000 on the market in Arlington. "All of the properties… come with at least one parking space," writes Amy Rose Dobson of Curbed. "Most of them have just entered the market within the past week and will probably move fast." [Curbed]
Flickr pool photo by Wolfkann
Eight-time Major League Baseball all-star Darryl Strawberry will be coming to Clarendon next week to talk sex, drugs, rock-n-roll and God.
Strawberry — who struggled with drugs, prostitution and other vices before he found God in 2006 — will host a sermon at 11:00 a.m. on Sunday, Sept. 23, 2012, at the Church at Clarendon (1201 N. Highland Street).
The church issued the following press release about the event.
On Sunday, September 23, 2012 at 11am, the Church at Clarendon, located at 1201 N. Highland Street in Arlington, welcomes 8-time All Star and 4-time World Series champion Darryl Strawberry to address the question, "What does God have against sex, drugs, and rock-n-roll?"
Strawberry's sermon is the third in a five week series the Church has titled "Blunt Questions" in which the Church challenges believers and non-believers to honestly wrestle with tough questions of faith. All are welcome to participate in the discussion.
An athlete perhaps known as much for his controversial behavior as for his legendary baseball, Strawberry will speak from a unique and personal perspective. "I was once very lost and tormented but now I am found and free in Christ Jesus. I want everyone to experience the saving and transforming power of Jesus Christ," notes Strawberry in discussing his current life purpose.
Prior to Strawberry's sermon, the Church will open its doors on Saturday, September 22 from 11am to 5:30pm to offer water and a cool place of rest for those visiting the Clarendon Day neighborhood festival. This year's festival is the first since the church re-opened its doors at the Highland Street location in the heart of Clarendon after more than 2 years in exile while the building was torn down and rebuilt.
"As a church, we exist in large part for those that are not yet part of us—to connect with those outside our church and offer them something of the goodness of God," Pastor David Perdue said. "We're excited to be hosting Darryl Strawberry because he is a well-known star who can also address a question that many people struggle with."
The public engagement continues on Monday, September 24, when the John Leland Theological Seminary, a ministry partner of the church, hosts the 40th Annual Faculties' Convocation of the Washington Theological Consortium on the topic of Theology in the Public Square. Events run from 3:30pm to 7:30pm.
Four Mile Run Rapist Still on the Loose — Arlington County police are still looking for a man who raped a woman on the Four Mile Run Trail two weeks ago. Police are issuing warnings to women who use the trail, in English and in Spanish. Officers are also patrolling the trail on bike and motorcycles. [WJLA]
Marymount, O'Connell Teaming Up for Baseball Field — Marymount University will be adding a varsity baseball team to its athletic program after striking a deal with Bishop O'Connell High School to use the school's baseball field. Marymount will fund the renovation of the O'Connell's field to NCAA standards, in exchange for partial use of the field. Marymount hopes to have the baseball team ready for its first season by Spring 2014. [Sun Gazette]
N. Va. Critical to Obama Re-Election — The road to the White House runs through Northern Virginia, according to some political watchers. Experts say President Obama must score a big victory in Northern Virginia in order to capture the Commonwealth, one of three crucial, hotly-contested swing states. As a result, residents can expect a bombardment of political ads this fall. [WUSA 9]
Flickr pool photo by Damiec
Englin Admits to Affair — Del. David Englin (D), who represents parts of Arlington, Alexandria and Fairfax, admitted Monday night that he had been "unfaithful" in his marriage and that he's currently separated from his wife, political consultant Shayna Englin. The four-term House of Delegates member announced he will not seek reelection in 2013. [Sun Gazette]
Va. Residents Are 60 Percent of Nats Attendance — About 60 percent of those attending Nationals baseball games are from Virginia, compared to about 25 percent from Maryland and 15 percent from the District, according to internal team numbers. Eight years ago a group tried to bring the Nationals and the team's new stadium to Pentagon City, but ultimately objections from Arlington residents helped to sink the plan after concerns were raised about crowds and traffic. A large cluster of office buildings is now proposed for what could have been the site of the baseball stadium. [Washington Post]
Ben & Jerry's Free Cone Day — From 12:00 to 8:00 p.m. today, Ben & Jerry's locations will be celebrating the company's 34 years in business by giving away a free scoop of ice cream, sorbet and frozen yogurt. Unfortunately, local ice cream lovers will need to get their free scoops in D.C. or Alexandria — there are no participating Ben & Jerry's locations in Arlington County. [Ben & Jerry's]
New Legal Programs in Ballston — The Ballston-based Court Reporting Institute is offering four new court reporting programs for those seeking a legal career. The programs include Legal Assistant, Voice Writing, Electronic Evidence Discovery and Certified Shorthand Reporter. The accelerated 120-hour Certified Shorthand Reporter course trains students to record notes and dialogue at a rate of 225 words per minute.
Photo courtesy Andrew Clegg
The George Washington University baseball team christened its new $3 million field at Barcroft Park (4200 S. Four Mile Run Drive) last Friday. The team might have lost the first game at its newly-renovated home field, but it was a victory for a Division I athletic program that finally has a Division I-caliber field.
"It was a recreational field before," coach Steve Mrowka told the GW Hatchet. "You couldn't really have a solid game there."
Now, the field has artificial turf, full-sized dugouts and more standard playing dimensions. Mrowka told the Hatchet that he believes the field will improve the team's play and help with recruitment.
The Arlington County Board approved the plan to renovate Barcroft Field #6 in September, in an agreement with GW that called for the school to pay for all renovations and to split maintenance costs 25/75 with the county. While GW is given priority on the field for games and practices, it is open for use by the community at other times (about 75 percent of playable hours).
Though the team is playing there now, work on the field is not complete. Construction crews are still busy adding features like 500-person seating capacity seating, a new concessions area, permanent dugouts and a reconfigured parking lot.
GW will be playing home games against Shepherd at the field on Saturday and Sunday, starting at 1:00 p.m. See a full game schedule on the GW Baseball website.


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open source software
Every week, I tally the numbers and listen to the buzz to bring you the best of last week's open source news and stories on Opensource.com. Week of August 29.
It's one of the most basic lessons we teach our children when they're young—the idea of sharing their toys with others. We try to instill in them the values of giving up control and playing alongside others. Of finding joy in a shared toy. We recognize the importance of this idea, and we work hard... Read more
Mark Bohannon gives an overview of some new open initiatives by the U.S. federal government, including the Digital Services Playbook and the U.S. Digital Service. Both focus on a "default to open" mentality.
Organizations large and small are changing their approach to open source software (OSS). Increasingly, OSS is being viewed as more than just a development tool, but as a strategic asset. And the implications of this change are becoming clear.
Pia Mancini discusses the challenges DemocracyOS faces and how Democracia en Red is planning to overcome them
Bird-I is an app to manage your location and your friends, plus an alarm for emergencies.
The beauty of open sourcing development resources is that a single library or wrapper, once released to the world, can be integrated and built out by thousands of other people. And because the future of any new platform depends on what people can do with it, over the past few months Leap Motion has... Read more
My journey from bench scientist to open science software developer and how I develop better tools for open, reproducible scientific research.
In this interview with Pia Mancini, I investigate the technologies and background of DemocracyOS, a platform for open government.
An open source project's website is the main gateway for potential users and contributors to learn about your project, and it assists existing community members to contribute to the project. But it has to do it right. Does your website clearly present your project, its goals and status, and assist... Read more


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Select a design, then select the device that you would like to style.
Great experience, from great selection to the quick delivery. Will recommend and return. Thanks.Tifani McCann, United States
iStyles is a fashion accessories provider for consumer electronics such as the iPhone. With coverage for 815 devices and a collection of 1695 designs, iStyles is the global one-stop brand that people turn to when they want to dress up their gadgets, to differentiate themselves from the crowd and to be stylish.
Subscribe and get 25% off your next order!
Thank you for subscribing! We need to confirm your email address. To complete the subscription process and redeem your discount voucher, please click on the link in the email we just sent you.


text:
I was a speaker on National Review magazine's post-election cruise from San Diego down the west coast of Mexico and back -- an exhilarating week with several hundred congenial conservatives, plus Bill Buckley and a number of towering experts such as professor Bernard Lewis of Princeton, perhaps America's foremost expert on the Islamic world.
The fact that the trip was being taken in the immediate wake of the Democrats' capture of both Houses of Congress didn't dampen the spirits of this bunch of conservatives as much as you may imagine. Many of them saw it coming; most of them thought the Republicans' wounds were largely self-inflicted; and practically all of them were confident the GOP will recover as soon as soon as it reconnects itself to the conservative principles that put it in power in the first place.
My only reservation about Holland America's generally splendid service had to do with its choice of news programs available on the TV sets in every cabin. For a good many years the line has seen to it that CNN is available, and in the days when CNN was the only existing cable news channel that was fair enough. But Fox News Channel has made its appearance in recent years, and has promptly far surpassed CNN in the ratings. This may well be owing to CNN's ever more liberal bias, particularly when compared with Fox's allegedly conservative coverage.
It would be no great ordeal for Holland America to add Fox's news coverage to CNN's on its cruise liners, but of course it would cause an uproar among militant liberals, who not only prefer CNN but would like to strangle Fox in its crib if they could. Thus far, therefore, Holland America has preferred to annoy its conservative passengers in this important regard.
A good example of what we conservatives have to put up with from CNN occurred during our cruise. CNN knows very well, of course, that popular opposition to the Iraq war is heavily fueled by the casualty lists. It is true that combat-related deaths, at around 2,800, are still, after 3-1/2 years, fewer than those sustained in the World Trade Center and the Pentagon on the morning of Sept. 11, 2001, alone -- compared with nearly 60,000 in Vietnam, and an average of 6,000 a month for 40 months during World War II. But the figure for Iraq balloons to a more impressive 21,000 if you add combat-related injuries, some of which are, inevitably, horrific. CNN promptly added them, horrific and otherwise, and a big fat "21,000" filled the TV screen.
William Rusher is a Distinguished Fellow of the Claremont Institute for the Study of Statesmanship and Political Philosophy and author of How to Win Arguments .
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Rush Limbaugh defends Rubio's debate answer: He's absolutely right that Obama knows what he's doing | Allahpundit
Time for Team Hillary to break out the gender card for young liberal women who prefer Sanders | Allahpundit


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Slow Food St. Louis and the Schlafly Bottleworks present SLOWednesday after the Schlafly Farmers Market. This month, the group will screen 'Growing Cities' about the current state of urban farming in the United States.
Phone: 314-241-2337
EventList powered by schlu.net
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If you have questions or need to contact KDHX, visit our answers portal at answers.kdhx.org.


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Phoenix Bodyshot by gb2kWatch
©2012-2016 gb2k
*Ink + Markers on 11"x17" bristol stock
Great drawing of Phoenix! Nice colors and backround! Great pose and facial features!
Sexy pose for a woman with great features.
She will never die lol
Really NICE
That sexy pose from a HOT lady!
good job
I really like this one
Your 3/4 body shots are hot enough, GB, but your fully bodies are the best! Hope to see more pics like this!
Dang this is super hot!
Hottity hot!
Another great piece. I just had to fav it.
oh so cool
I love how you do metal textures. It's awesome
Way cool pose and awesome styley/colours!
nice one
That's one nice body
This babe is the hottest of X-Men! . Very impressive work yet again.
yum. Too bad she was a very very bad-girl back when I read the X-Men comics.
very nice
First time I saw Jean Grey in this outfit in the 90's cartoon series. She blew my mind away.
Fantastic work!
Fantastic work!
Very styling!
Everybody loves an unstable nearly omnipotent red head...right?
Absolutely love how you did the yellow/gold bits. Very impressive!
awesome , nice body n_n


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This is an archived publication. It may be useful for researchers doing further work in this area as well as those developing improved testing and design procedures and is included because of its historical value
Title: Hydraulic Stability of Articulated Concrete Block Revetment Systems During Overtopping Flow
FHWA Publication Number: RD-89-199
NTIS Publication Number: PB90-266156
Publication Year: 1989
Document Links: PDF (ISDDC)
PDF NOTE: The PDF file may have been produced from html files which were prepared for use with web browsers. The PDF files created from HTML are intended for electronic viewing only.
Problems Downloading ISDDC files? Please contact ISDDC directly at http://isddc.dot.gov/


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1. Remember the band-plays-on scene from "Titanic"?
2. It really happened. And now the violin played by bandmaster Wallace Hartley has been found and positively identified, according to British auction house Henry Aldridge & Son.
The auction house worked tirelessly with forensic scientists, Oxford University, and a Hartley biographer to identify the violin, according to the Associated Press. The instrument is still in good condition, despite being water-stained and cracked.
4. According to the auction house, Hartley's body was found strapped to a large valise with the violin inside. It then went from his fiancee to a Salvation Army and a violin teacher.
Wallace Hartley
5. The relic will go on display at Belfast City Hall at the end of March.
The Titanic was built at the Harland and Wolff shipyard, less than a mile from city hall.
- Former Virginia Gov. Jim Gilmore is dropping out of the race for the Republican presidential nomination. He got 133 votes in New Hampshire.
- MLB issued its first-ever lifetime ban for performance enhancing drugs to New York Mets' Jenrry Mejia.
- And how well do you know what happened in the news this week? Take our quiz.


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Red Velvet Cheesecake Recipe
4.5 27
Festive and oh-so-good, this cheesecake will become a fixture on your Christmas dessert menu. The red velvet filling is spiked with cocoa, topped with cream cheese frosting, and baked in a chocolate cookie crumb crust. —Karen Dively, Chapin, South Carolina
- 17 chocolate cream-filled chocolate sandwich cookies, crushed
- 1/4 cup butter, melted
- 1 tablespoon sugar
- 3 packages (8 ounces each) cream cheese, softened
- 1-1/2 cups sugar
- 1 cup (8 ounces) sour cream
- 1/2 cup buttermilk
- 3 tablespoons baking cocoa
- 2 teaspoons vanilla extract
- 4 eggs, lightly beaten
- 1 bottle (1 ounce) red food coloring
- 1 package (3 ounces) cream cheese, softened
- 1/4 cup butter, softened
- 2 cups confectioners' sugar
- 1 teaspoon vanilla extract
- Place a greased 9-in. springform pan on a double thickness of heavy-duty foil (about 18 in. square). Securely wrap foil around pan.
- In a small bowl, combine the cookie crumbs, butter and sugar. Press onto the bottom of prepared pan.
- In a large bowl, beat cream cheese and sugar until smooth. Beat in the sour cream, buttermilk, cocoa and vanilla. Add eggs; beat on low speed just until combined. Stir in food coloring. Pour over crust. Place springform pan in a large baking pan; add 1 in. of hot water to larger pan.
- Bake at 325° for 60-70 minutes or until center is just set and top appears dull. Remove springform pan from water bath. Cool on a wire rack for 10 minutes. Carefully run a knife around edge of pan to loosen; cool 1 hour longer. Refrigerate overnight. Remove sides of pan.
- For frosting, in a small bowl, beat cream cheese and butter until fluffy. Add confectioners' sugar and vanilla; beat until smooth. Frost top of cheesecake. Refrigerate until serving. Yield: 16 servings.
Originally published as Red Velvet Cheesecake in Country Woman December/January 2012, p42
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I know that photoshop uses a ton of resources and RAM but it was remarkably smooth and efficient with Leopard. Why is Snow Leopard so different? I can't even use it anymore and I can't afford to purchase a new mac. Is there a fix or do I need a ton of more RAM?
I may have found my own solution. I didn't know that CS5 had an update to address a lot of bugs. I'm betting that Snow Leopard is one of them. The version is 12.4.
Retrieving data ...