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Why did you choose this product?
This CD has changed the way I look at my thought processes. I had no idea how negative I was being, and now I'm on the road to leading a truly joyful life because practicing the principles in this book really give you "the power" to create your own reality. My biggest challenge is health, and I've already had some miraculous experiences with self-healing just by putting into practice what I've learned from this book. "Imagine, feel and receive" is my new daily mantra!...
The Secret (CD-Audio)
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ITEM#: 10470217You hold in your hands a great secret...
It has been passed down through the ages, highly coveted, hidden, lost, stolen, and bought for vast sums of money. Fragments of this Great Secret have been found in the oral traditions, in literature, in religions and philosophies throughout the centuries. It has been understood by some of the most prominent people in history: Plato, Galileo, Beethoven, Edison, Carnegie, and Einstein, along with other renowned inventors, theologians, scientists, and great thinkers.
For the first time, all the pieces of The Secret come together in an incredible revelation that will be life transforming for all who experience it.
In this audiobook you will discover The Secret . . . and you will learn how to have, do, or be anything you want. You will learn how to use The Secret in every single area of your life. You will hear from modern-day teachers -- men and women who have used The Secret to achieve health, prosperity, relationships and happiness. They share their incredible stories of using The Secret to eradicate disease, acquire massive wealth, overcome obstacles, and achieve what many would regard as impossible. Through them, you will begin to understand the hidden, untapped power that is within you, and the true magnificence that awaits you in life.
To experience the film version of The Secret visit www.thesecret.tv
|BODY, MIND & SPIRIT / Inspiration & Personal Growth
|Publisher Date
|2006-11-28 00:00:00.0
|Simon & Schuster
|Copyright Year
|5.75 in
|5.25 in
|1.0 in
|Unit weight
|0.32 lb
|Volume Number
|Edition Detail
|Byrne, Rhonda
|Country of Origin
|United States
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Customer Reviews
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This CD has changed the way I look at my thought processes. I had no idea how negative I was being, and now I'm on the road to leading a truly joyful life because practicing the principles in this book really give you "the power" to create your own reality. My biggest challenge is health, and I've already had some miraculous experiences with self-healing just by putting into practice what I've learned from this book. "Imagine, feel and receive" is my new daily mantra!Read More
The Secret CD
Good quality and good price, value and fast deliveryRead More
Just good reminders
Taped interviews and personal reflections demonstrate and remind us how practicing a positive attitude, faith, awareness, and education can improve our lives. The basic messages are the stuff of an ideal existence and simple tools to take charge of and appreciate your station in life. The repetitive style of delivery is somewhat annoying; and I think the program could have been cut substantially without damaging the message. Could be viewed as a remake of The Celestine Prophecy, by James Redfield.Read More
Great item, just as described, very satisfied, thank you very muchRead More
After receiving this product, my life has changed tremendously. I mow have the power and tools to be happy and successful!Read More
Most Helpful
Amazing book!
The audiobook is a must have for your "Drive Time Univeristy"! The Secret is one of the most profound movies to hit the market. Listening to it on CD helps sink in the principles of The Law of Attraction. Anyone who is interested in creating a life they love will want this CD! In fact get two copies, so you have a back up, just in case you wear the first one out! The teachers that contributed to The Secret are successfull, wealthy, spiritual, healthy men and women, who so generously have shared their wisdom with those seeking to make a difference in their lives and the lives of others!Read More
Questions & Answers
it's a secret, lol
Love the book. Want to share with a non-reader.
recommendation from a coworker...
Recommended by a friend.
Looked interesting
It's a gift
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text:
What do you sleep on?(10 Posts)
Tailtwister Sun 02-Feb-14 17:00:57
AngryFeet Mon 03-Feb-14 11:45:07
Blu Mon 03-Feb-14 15:30:03
Thumbcat Mon 03-Feb-14 17:02:39
Tailtwister Tue 04-Feb-14 13:26:31
mummyofwilliam Thu 06-Feb-14 19:16:54
WelshMoth Fri 07-Feb-14 18:02:20
DumSpiroSpero Thu 13-Feb-14 21:16:07
JourneyToThePlacentaOfTheEarth Fri 14-Feb-14 19:41:16
Wolfiefan Fri 14-Feb-14 19:47:59
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Realized Gains and Losses Calculation
Acumatica automatically calculates realized gains and losses from foreign currency transactions entered into the other financial modules. This can include receiving payments from customers, issuing payments to vendors, and transferring funds between accounts.
Unrealized Gains and Losses Adjustments
Acumatica can compute and create adjusting entries for unrealized gains and losses associated with AP liabilities and AR assets recorded in foreign currencies. Before closing the financial period, you can run the unrealized gain and loss calculation process to prepare auto-reversing entries in the GL for all open documents recorded in foreign currencies.
Financial Statement Translation
Acumatica provides financial statement translations so you can manage subsidiaries that operate in a foreign currency or submit your financial statements in a foreign currency. You can define the appropriate rules for translating GL account balances and Acumatica will perform the translation of the trial balance into a foreign currency following FASB-52 standards. Translation gains and losses are automatically calculated. You can automate the consolidation of financial statements from multiple subsidiaries by using this functionality in combination with the consolidation features in the GL module.
View PDF data sheet on consolidation.


text:
When NBA basketball player Jason Collins recently announced to the world that he's sexually attracted to other men, Michelle Obama tweeted, "So proud of you, Jason Collins! This is a huge step forward for our country. We've got your back!" Jason even got a personal call from the president.
But even as the Obama administration is busy validating lifestyle choices and values that fly in the face of sexual morality, this president is just as busy undermining the free exercise of the very Christian faith that dares to acknowledge morality even exists.
President Obama's Pentagon recently released a statement threatening military personnel: "Religious proselytization is not permitted within the Department of Defense … Court Martials and nonjudicial punishments are decided on a case-by-case basis."
Although the Pentagon has walked back its new anti-Christian "proselytizing" policy within the realm of public relations (a policy drafted in concert with foul-mouthed atheist and anti-Christian bigot Mikey Weinstein) the DoD has yet to offer evidence that it intends to walk it back within the realm of application.
Merriam Webster's defines "proselytization" as an attempt "to recruit or convert especially to a new faith, institution, or cause." Sounds an awful lot like the Pentagon is threatening to court-martial any service member who obeys Christ's central command to "Go into all the world and preach the gospel to all creation" (Mark 16:15). Hard to read it any other way.
And so, to any member of the armed services who is harassed, demeaned, reprimanded or charged by this Obama Pentagon with obeying Jesus – with endeavoring to "recruit or convert" others to His exclusive saving grace – we at Liberty Counsel are delighted to say, "We've Got your back!" Free of charge. Pro bono legal defense.
How are we able to do this? Through the generous financial and prayer support of more than 1.2 million Liberty Counsel donors and supporters. So please, get this information to every member of the armed forces you know. If you (or they) are being persecuted in any way, at any time for sharing the love of Christ, let us know. Call us right away at 1-800-671-1776, or by emailing us at email@example.com.
Matt Barber is founder and editor-in chief of BarbWire.com. He is an author, columnist, cultural analyst and an attorney concentrating in constitutional law. Having retired as an undefeated heavyweight professional boxer, Matt has taken his fight from the ring to the culture war. (Follow Matt on Twitter: @jmattbarber).


text:
The great thing about Microsoft webcasts is that they're archived. So even if you miss the upcoming live webcast on "Implementing SCCP 2006", you can still watch it at your leisure (well, as long as your leisure includes a broadband connection).
This also means that you can also watch all the previous webcasts about SCCP. So it seems like a good idea to have a list of them somewhere. Here's that list:


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Collings D3 with Torch Peghead Inlay #19118
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The square-shouldered 14-fret dreadnought is the most popular steel-string acoustic guitar body shape in the world. While Collings is certainly not the only company to build them, we bring a new tonal clarity to the depth and warmth usually associated with such a large, deep-bodied guitar. Although its bass response makes the dreadnought ideal for vocal accompaniment, Collings versions are also often employed by bluegrass flatpickers who must compete with inherently louder instruments such as banjos and fiddles. The wide range of woods and neck sizes offered allow dreadnought fans to find a Collings model ideally suited to their playing style and tonal preference.
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Select Sitka spruce, East Indian rosewood back and sides, Grained ivoroid binding, Black/white wood strip purfling, Multi-color mosaic back strip, Select abalone rosette, Pre-war scalloped bracing, Tortoise-style pickguard, High gloss nitrocellulose lacquer finish, Mahogany Neck, Bound fingerboard and double bound peghead, Torch Inlay, Rosewood or ebony peghead overlay, Inlaid Collings Logo, Ebony fingerboard and bridge, No fingerboard inlays, Fully adjustable truss rod, Bone nut and drop-in saddle,, Ebony bridge pins and end pin Gold Waverly tuners.
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By John T. Bennett - 07/14/11 08:59 PM EDT
Senate Armed Services Committee Ranking Member John McCain (R-Ariz.) will oppose a Pentagon request to transfer $264 million from other accounts to begin paying for a new $771 million cost spike, a Senate aide tells The Hill.
McCain, long a critic of the Lockheed Martin-led program, and one of the Senate's most outspoken and blunt members let his feelings about the new cost spikes be known in the same tweet. "Outrageous! Pentagon asking for $264M down payment now. Disgraceful," he tweeted.
The $771 million overrun covers the first 28 F-35s the Pentagon is buying. The Pentagon informed lawmakers on Monday of a need to move monies within its budget for a $264 million down payment, as the aide called it, via a reprogramming request sent across the Potomac River in June.
"I intend to strongly oppose future 'reprogramming requests' unless they can be fully justified to the American taxpayer," McCain said later Thursday in a statement.
"Any new requests for additional funding by the Department of Defense should be submitted to the Congress for formal review, debate, amendment, and approval by the appropriate defense committees and the full body of both Houses of Congress," McCain said. "This should be done as a request for emergency funding or be funded through supplemental appropriations legislation by Congress and offset against commensurate, identified cuts in spending."
McCain and SASC Chairman Carl Levin (D-Mich.) wrote Defense Secretary Leon Panetta on Thursday to raise their concerns about the cost spike.
-- This story was updated at 5:23 p.m.


text:
What don't you hear from the media about a shooter on the loose
Hour 1-
What are we learning about a former cop who has gone on a shooting rampage in LA? What isn't the media saying about him and his manifesto?
Why is one black guy dressing as a KKK member?
Length 14:58


text:
See definition in Oxford Advanced Learner's DictionaryLine breaks: in|sinu|ation
Pronunciation: /ɪnsɪnjʊˈeɪʃ(ə)n/
Definition of insinuation in English:
An unpleasant hint or suggestion of something bad: I've done nothing to deserve all your vicious insinuations [mass noun]: a piece of filthy insinuation
More example sentences
- The thefts were calculated and professional, and the staff were quick with unpleasant insinuations when the evil deeds were pointed out.
- But there are just hints and insinuations that if I stick around long enough there might be a plot later.
- Far be it from me to stoop to petty insinuations and suggestions, just to illustrate a point.
implication, inference, suggestion, hint, intimation, imputation, innuendo, reference, allusion, indication, undertone, overtone;
Definition of insinuation in:
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text:
I've always loved the monuments at Yankee Stadium but they don't respond to me when I talk to them. Fortunately, Citi Field has its own historical symbols, and one of them is Tracy Nieporent, a Mets fan since 1962, who is alive and well and a fixture at most home games.
Read Whole Story
It may just be a game. It might just be an illusion of hope. It might be too early in the season to order playoff tickets. But it's starting to feel a lot like 1969. Or 1986.
Frank Zappa taught us "you are what you is." Former high-school and college jocks oft fall prey to the corresponding ravages of time as do their ex- rocker counterparts.
I have never forgotten that period of time and hoped that it had faded, that our nation had changed.
Forget steroids. Forget Frank McCourt's mismanagement of the Dodgers. The biggest scandal in baseball at the moment is the Baseball Hall of Fame's failure -- for the fourth time -- to induct Marvin Miller, who freed players from indentured servitude.
With the ticket money squirreled away, the thousands not spent on parking, plus the cash I wouldn't have squandered on $5 waters and $6 pretzels, I'd now be in position to become an equity partner in the NY Mets.
In this summer of our discontent, a season of buckling banks and wheezing newspapers, it might be well to remember that as far as crisis years go, 200...
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- Origin: China
Product Description
Please search sinsansports in "google" to find our site. More sunglasses item please contact us. We manufacture and supply all kind of cheap sunglasses and expensive sunglasses, including metal sunglasses, plastic sunglasses, brass sunglasses, nickle sunglasses, acetate sunglasses etc.


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Guest Reviews
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"Great Getaway"
Great weekend getaway! This hotel is newly refurbished. They did a great job. The decor is beautiful. The rooms were unbelievably spacious. One queen bed in its own room and the other upstairs in a little loft. A small kitchenette with a sink, mini fridge and microwave. Pull out sofa bed. Perfect for our family of 5. Ice cream delivered to the room in the early evenings was enjoyed by the kids. The location is perfect. The Pirate restaurant is only a short walk away and is a must to dine at. Right on the river walk. A fabulous stay and would not look further for accommodation. The Inn has a lot of history too which adds to the Savannah experience. Took a 30 minute drive to Tybee beach and enjoyed a day swimming in the ocean,walking the almost 200 steps to the top of the lighthouse (my 3 year old made it without any help), walked the peer eating ice cream. The staff at the Olde Harbour inn were very helpful. We also liked that breakfast is included. At night they offer free wine and horderves in the lobby. The reviews on the hotel were good and that was why we chose to stay here and we are very happy we did!Reviewed by PVClan on 4/12/2015 via TripAdvisor
"Wonderful Stay!"
This was the first mother/daughter trip we had taken and I really wanted it to be special. The accommodations were clean, roomy and comfortable. The location was great! With that being said, it was the staff that really made our stay great! They went over and beyond to not only provide great service, but they always did it with a smile on their face and in their voice!Reviewed by anitac1974 on 04/11/2015 via TripAdvisor
"Beautiful "olde" hotel down by the river."
This hotel has beautiful suites, free wine & apps in the lobby every evening and a perfect spot right down by the river.Watch the dolphins swim by the 'waving lady'. Restaurants and shops, open markets and trolley tours made our stay amazing in Savannah. And....ice cream bars for our evening turn down! A great full breakfast included. Definitely has southern charm.Reviewed by Colleen B on 04/1/2015 via Trip Advisor
Neat Renovation of an Old Historic Building
"Thanks for hosting us during our stay in Savannah. The hotel was a neat renovation of an old historic building. Our room was much larger than expected. We would be happy to return to the Olde Harbor Inn. "Reviewed by Edgar S. on 10/19/15 via Website
Wonderful Stay
"Wonderful stay. Staff was professional and very courteous. Beautiful rooms and loved the wine and cheese reception. Will be back."Reviewed by Brent F. on 11/02/15 via Expedia
Staff Incredibly Friendly
"The staff was incredibly friendly and welcoming. They were knowledgeable about the area and had some great recommendations. We enjoyed our stay very much."Reviewed by Elizabeth G. on 11/07/15 via Website


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Parents need to know that "Rumour Has It" is another powerful commentary about relationships and how they can go wrong from the sweet and sassy Adele. There are some implications of cheating and messing around with someone who is taken, but in the end the singer sees the light and moves on without him. Other than the mature theme, there's nothing inappropriate for kids here.
On the heels of a series of hits like "Rollin' in the Deep" and "Someone Like You," Adele packs a punch with "RUMOUR HAS IT." The drum-driven bluesy-pop single about deciding to leave an unhealthy relationship lets the singer show her strength, sassiness, and gift for making songs you love to sing.
It's hard to believe that anything could top "Someone Like You," "Rollin' in the Deep," or "Set Fire to the Rain," but Adele's blues-kickin' "Rumour Has It" raises the bar and proves why this brazen beauty can spit fire and make you cry all in one song. As she sings, "Bless your soul, you've got your head in the clouds / You made a fool outta me / And, boy, you're bringing me down," it's easy to picture a pencil-skirt-wearing Adele ready to kick the dirt up in a lover's face as she turns on her heels and walks away. Once again, Adele proves why she should be the reigning queen of poetry put to music, and rumour has it, she is.
Families can talk about the enduring allure of Adele. What makes her and her music so relevant and popular today? Is it her voice, the lyrics, the music? Or the overall package?
What does it mean to be in a healthy relationship? What are some signs that could mean potential trouble in a relationship? Does Adele do a good job writing about some of these issues in her songs?
Why are so many pop songs about relationships? Do other genres focus on this topic, too?
|Release date:
|February 29, 2012
|XL Recordings
|Parental advisory:
|Edited version available:
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Healthcare is broken but fixable
February 10th, 2016: Our healthcare system is broken but it is fixable
February 7th, 2016: The complexity of political issues and the absence of countervailing forces gives more power to special interests
The Xerox Announcement
January 30th, 2016: Xerox' announcement of a split into 2 companies shows the challenge of reinventing a firm with a declining core business
A Relaunch of My Web Site- December 2015
December 24th, 2015: As my family and I celebrate the holidays this year, we truly feel that we have gone through a rebirth from the many challenges we have faced in the past few years.
The Future of Passenger Cars
December 12th, 2015: Recently, I traded in my 10-year-old Lexus for another Lexus. If I keep my new car for the next 10 years, the next car I buy might be a self-driving or autonomous car.
A contrarian perspective on our so-called skills gap
November 26th, 2015: Over the past few weeks, as I have thought about the issue of job readiness,
Limits on Presidential Power and Ways Around Them
November 23rd, 2015: During President Obama's recent interview with sports columnist Bill Simmons, he made quite revealing comments about his frustration with the limits of Presidential power.
We must pay more attention to high concentrations of risk
November 9th, 2015: Businesses have in-depth knowledge and core competency with respect to the markets in which they participate.
Why Americans are Angry at Politicians
October 31st, 2015: The political party establishments, expert political commentators and the media are clearly bewildered by the staying power of anti-establishment candidates like Donald Trump, Dr. Ben Carson, and Bernie Sanders.
WHY I OPPOSE THE PUBLIC OPTION (I'VE HEARD THIS SONG BEFORE) - October 2009
October 12th, 2015: In the October 21, Wall Street Journal, there was an article entitled "Japan Post Goes in New Direction." Reporters Atsuko Fukase and Allison Tudor reported on a change in leadership and the potential reversal of the government's..


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Finish first with the DeSoto™ Forza Riviera Tri Short. A serious short for a serious athlete. Now with pockets!
Good for Ironman distance
Dries pretty quick after swimming. Padding is perfect for cycling, esp iron distance. The padding does not rub or irritate while running. Pockets are large enough for several gels, tube of sun screen, etc.
Memphis, TN
Fit: Feels true to size
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clarify dusty ?
Originally posted by Dustytoad
I have met Demons. I don't need faith to know that I would do everything in my power to stay away from them for all time if I can.. I met demons when I was little.
Originally posted by Sinny
Definition of a Demon: An evil spirit or Devil thought to posses a person, or a cruel and destructive thing.
I have a hard time swallowing all this "Demon" stuff.
At least in a religious sense.
I'm spiritual, but my spirituality could never be bound, conformed or shackled within conformist religion.
I don't believe there is any such thing as "evil".
Now, negative, is a word I can use.
I could sense the resentment toward something at the heart of many of your posts.
Originally posted by roblot
reply to post by OMsk3ptic
Wasn't that from an X-Files episode? Remember? The strange entity that'll form into the physical shape of your biggest fear?
Originally posted by tvtexan
reply to post by Skyfloating
I know from personal experience that there are inter-dimensional entities who are posing as aliens
One thing is for sure, there are waaaaaay too many people with these demon/alien/entities visitation stories for it all to be some type of mass-delusion.
edit on 24-9-2012 by tvtexan because: (no reason given)
Originally posted by NullVoid
reply to post by Zane Zackerly
but with all our scientific community, they look the other way, chicken and ego perhaps.
Challenge to scientific community:
Make me a biomechanical or something that these "soul" can posess and make it push a button.
The entire universe. (except territory where other species have done this first)
Originally posted by reject
reply to post by Zane Zackerly
what was the point of it all?
Did it ask you to do something evil?
Why would something trying to win you over to its side show you how undesirable it is?
It didn't offer you anything at all to entice you?
It all seems like an exercise in futility that made no sense at all except to drive you into the side of its "opponent."
Originally posted by 14221
For all we know they may be angels and offer a better way of life than what we have here!


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A sophisticated yet simple design on the 60" Venica Double Vanity brings the natural variations in teak to the forefront. This spacious double vanity allows two people to enjoy their morning routine together.
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- Product Finish: Teak
- Material: Teak
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- Number of Basins: 2
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FABULOUS WELL CONSTRUCTEDThere's nothing bad about this product whatsoever. The quality of teak is incredible the shipping was impeccable
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In this brilliant and groundbreaking book, New Yorker writer Malcolm Gladwell looks at why major changes in our society so often happen suddenly and unexpectedly. Ideas, behavior, messages, and products, he argues, often spread like outbreaks of infectious disease. Just as a single sick person can start an epidemic of the flu, so too can a few fare-beaters and graffiti artists fuel a subway crime wave, or a satisfied customer fill the empty tables of a new restaurant. These are social epidemics, and the moment when they take off, when they reach their critical mass, is the Tipping Point.
In The Tipping Point, Gladwell introduces us to the particular personality types who are natural pollinators of new ideas and trends, the people who create the phenomenon of word of mouth. He analyzes fashion trends, smoking, children's television, direct mail, and the early days of the American Revolution for clues about making ideas infectious, and visits a religious commune, a successful high-tech company, and one of the world's greatest salesmen to show how to start and sustain social epidemics.
The Tipping Point is an intellectual adventure story written with an infectious enthusiasm for the power and joy of new ideas. Most of all, it is a road map to change, with a profoundly hopeful message, that one imaginative person applying a well-placed lever can move the world.
Don't miss any of Malcolm Gladwell's books, articles, and interviews.
©2000 Malcolm Gladwell; (P)2005 Time Warner AudioBooks
"A fascinating book that makes you see the world in a different way." (Fortune)
"Interesting, but where's the info?"
This was an interesting book, but too much time was devoted to detailed accounts of studies and anecdotes, which seemed endless. I also kept waiting for a summary of the author's view of what we should learn from these stories, but it seemed to never come. I always was disappointed. For example, after several anecdotes about "stickiness", the author concluded by saying, you can make your message have impact if it has stickiness. The key is for you to find it (i.e. what will make it stick). Well, my reaction is, no kidding! I was hoping for a little more insight rather than constant stories.
Maybe I expected too much and read it for the wrong reason. I was hoping to obtain another view providing insight and hints and opinions on how to make a message persuasive and have impact. I was very disappointed because that info was lacking in the book.
In addition,the book should have been read by some one with a little more inflection and intonation. The reader in this case, who I think was the author, put me to sleep and after about 2 hours got on my nerves. It seemed as though he was falling asleep as well. The 3 hour CD seemed much, much longer.
"Gets Mental Gears Turning"
I'm glad I bought and listened to this book. Although no gound-swell of wisdom overtook me, the book caused me to think. What else can you really expect these days. Easy listen and well-told.
"Insightful, Important Information"
Gladwell gives us the microscope on why people trend in one direction or another. I received the hardcopy from a friend who is the director of a major CA hospital. I have since shared it with CEO's and COO's and the feedback I've received is that it is perhaps the most important book anyone in business could read.
"Clever premise, zero insight"
Compared to similar, better researched and more tightly written and argued books like "Freakanomics" or "The Wisdom of Crowds," this book comes off as something that a self-satisfied Ivy League student might write as his thesis paper. There are a lot of interesting ideas and facts, but other than some highly contrived arguments Gladwell half-heartedly tries to work in, there simply isn't much tying them to the central thesis.
In short, the book is just a bunch of disjointed anecdotal examples of "tipping points" which Gladwell tries to buttress with a lot of interesting and tangential information, presumably hoping the reader be entertained enough to fail to notice that he's not actually proving his point in any substantial way. Apparently, he's been very successful at this, but it doesn't change the fact that the "Tipping Point" has plenty to SHOW, but pretty much nothing to SAY.
And the book simply ignores or dismisses in a sentence or two the broad social trends that were going on during specific "epidemics" that were probably as responsible for them as the narrow and inaccurate reasons Gladwell gives. For example, does anyone actually think that if extreme sports hadn't exploded in the 1990's Airwalk would have succeeded through clever advertising? Of course not. Yet Gladwell would have one believe that clever advertising taking advantage of "social innovators" and "mavens" were singlehandedly responsible for Airwalk's success. And "Freakonomics'" explanation for why crime in New York dropped is a LOT more compelling (and statistically disproves) Gladwell's argument that the NYC authorities successfully engineered an "anti-crime epidemic."
On the whole, this is the second glib, insubstantial and overrated book by Gladwell I've listened to, which seems like plenty.
This is an incredible book and narrated by the author whom has a beautiful voice and adds feeling to the words. I was enthrawled.
"Freakanomics has my vote."
I have the highest regard for Malcolm Gladwell from what I have read of him in the New Yorker. However, I found this audiobook nowhere near as interesting or wide-ranging as Freakanomics, which also tries to explain observed sociological phenomena. Both, for example, consider the marked drop in the homicide rate in the early 1990's in New York. Freakanomics uses extensive data to persuade the listener that it was part of a national trend precipitated by the 1973 Roe v. Wade decision which legaled abortion. This is far more persuasive than Gladwell's view that it was just consequence of a decision by a few New York gangleaders which then spread among the rest. Freakanomics is also superior because of the authors' humor and the more expressive way in which the book is read.
"Good topic ... excruciatingly long discourse"
The fundamental premise of the book (i.e. small things make a big difference) is sound and interesting. However, what detracts from the value of the book is the endless analogies and digressions to prove this fundamental premise. The book could have easily been 1/2 the size and not missed the point. Nevertheless well written and well read by the author.
"Phenomenal Book!!!"
This book is truly inspiration in all angles! A must read for everyone who has a desire to change the world into a better place
bronx kay
"Abridged Adaptation of Social Marketing Classic"
I had not realized that this was an abridged adaptation of the book until the publisher's outtro. It does contain all of the key concepts. The appendices are essential. i
My interests run to psychology, popular science, history, world literature, and occasionally something fun like Jasper Fforde. It seems like the only free time I have for reading these days is when I'm in the car so I am extremely grateful for audio books. I started off reading just the contemporary stuff that I was determined not to clutter up my already stuffed bookcases with. And now audio is probably 90% of my "reading" matter.
"Gladwell at his best"
There are brilliant insights here. About how trends start. About social networks. You even get the truth about six degrees of separation. Put it all together and it's a dazzling new perspective on how human society works. I only regret that I accidentally ordered the abridged version instead of the unabridged.
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Penally Abbey Country House Hotel3
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About the hotel
Penally Abbey is a Country House Hotel with views of the sea on the edge of the Pembrokeshire National Park. The pretty Medieval Walled Town of Tenby, with its cobbled streets, picturesque harbour, miles of golden sand and Blue Flag beaches is only a short distance away. The emphasis here is on good food, fine wines, friendly unobtrusive staff and comfortable accommodation In the winter you can relaxed before dinner in front of a roaring log fire.
In the summer there are extensive grounds to enjoy. Breakfast is waitress service and cooked to order. The three course set price dinner menu changes regularly. Everything we cook, we make ourselves: soups, pates terrine's,desserts are all made by our chefs.
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Deloitte refers to Deloitte Corporate Finance LLC, an SEC registered broker-dealer and member of FINRA and SIPC, which is an indirect wholly-owned subsidiary of Deloitte Financial Advisory Services LLP and affiliate of Deloitte Transactions and Business Analytics LLP. Investment banking products and services within the United States are offered exclusively through Deloitte Corporate Finance LLC. For more information, visit www.investmentbanking.deloitte.com. Plese see www.deloitte.com/xa/about for a detailed description of the legal structure of Deloitte LLP and its subsidiaries. Certain services may not be available to attest clients under the rules and regulations of public accounting.


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Genesis 41:45New King James Version (NKJV)
45 And Pharaoh called Joseph's name Zaphnath-Paaneah. And he gave him as a wife Asenath, the daughter of Poti-Pherah priest of On. So Joseph went out over all the land of Egypt.
New King James Version (NKJV)
Scripture taken from the New King James Version®. Copyright © 1982 by Thomas Nelson. Used by permission. All rights reserved.
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Laptop Request Form
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City Commissioner Jerry Libbin, president of the Miami Beach Chamber of Commerce, will open a campaign to establish a new, full-time city … [Read more...]
News: Commissioner Jerry Libbin Opens Push for New City Post of Condominium Ombudsman
News Commentary: Welcome to the Neighborhood
I thoroughly enjoyed the Third District Neighborhood Safety Walk led by the Miami Beach Police Department last Monday. Indeed, this humble … [Read more...]
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Waste Management trucks are collecting and transporting organic material from Publix stores in Miami Beach to a new Okeechobee Organics Recycling … [Read more...]
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As provided by Miami Beach City Manager Jorge Gonzalez What is a "destination resort?" In addition to offering limited gaming facilities, a … [Read more...]
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To the chagrin of some residents, no date is currently set for the commission hearing of a building proposal that might bring a strip club to North … [Read more...]
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Garden Fosters a Green and Healthy Option for Residents The city of Surfside dedicated their first new community garden last Saturday. The garden is … [Read more...]
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Local Miami beach artist, Enrique Gomez De Molina plead guilty to charges of wildlife smuggling on Tuesday. De Molina who has made a name for himself … [Read more...]
News: Casino Bill Won't Pass
Tallahassee Lawmakers Tell Beach Leaders The move to approve destination resort casinos in South Florida is unlikely to pass the state legislature … [Read more...]
News: Paul Pry Pages
City of Miami Beach Meetings By Anne Newport Royall TUESDAY MORNING BREAKFAST CLUB Tuesday, November 22 BUILD IT, (IF THE CITY … [Read more...]


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The Gastrointestinal (GI) Cancer Program at Penn Medicine's Abramson Cancer Center provides diagnosis and treatment for the full spectrum of GI cancers.
Our GI cancer specialists are nationally recognized for their expertise in providing the information, care, and support to help you each step of the way.
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Plant an Annuals Border
Everything looks better when it's framed, whether it's a picture or a garden path. Try wax begonias, lobelias, sweet alyssum, or low-growing petunias and pansies for colorful boundaries between the garden and the lawn or pathway. For sunny borders, experiment with dahlias. If your garden is shady, try caladiums, browallia, primrose, or dwarf bedding carnations.
Step 1
1. With a hoe or cultivator, loosen the soil before planting.
Step 2
2. Set plants along the front of the path. Space them the correct distance apart according to your plant species. Water well.
Wishbone flower
3. A few weeks later, the plants will have filled in the empty space. Wishbone flower (Torenia fournieri), shown in the photos, makes a charming edging for shady beds and borders.


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Rock and Roll Cowgirl Short Sleeve Dolman T-ShirtSKU: #8569867
Product Information
- Round up some homegrown style in this supercute Rock and Roll Cowgirl® top.
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This is an adorable top! It looks better though on the model than me...so had to return it.
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|inSANE VGA Teaser
Guillermo Del Toro's "inSANE" gets its first trailer, courtesy of this year's VGAs.
Jul 5, 2011
Duration: 00:39
Views: 4579
|Whatever Happened to Guillermo del Toro's inSANE and Devil's Third Following THQ's Demise?
|Mar 19, 2014
|inSANE: Guillermo del Toro in Talks with "Very, Very Big Company"
|Jan 07, 2013
|Guillermo del Toro's inSANE Could be Back On According to Rumours
|Nov 29, 2012
|Guillermo del Toro's inSANE Trilogy is Shelved by THQ
|Aug 07, 2012
|del Toro's inSANE Still Two or Three Years Away
|Aug 16, 2011
|View past articles


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text:
7982 Trust My Rage by SweetLittleVampireWatch
OMG, what a lineage.
Wouldn´t have managed it without DatNachtmaehre´s help; she deserves fan art for it.
But he´s beeeeeaaaauuutiful! I mean c´mon, look at him!
And before you ask – yep, partially inspired by MCU Loki. But I always loved the colour combination green and gold…My grandma used to have a golden fantasy necklace with green beads, and I was allowed to wear it as a kid when I was playing dress-up. It was my favourite thing, I always thought it was so pretty.
Name: Trust My Rage
Nickname: Rage
Color: Black laced tobiano peafowl
Genotype: EE aa nPwl nTl
Gender: Gelding
Age: 8
Build: 40 % Andalusian, 50% Friesian, 10% Draft
Height: projected to be up to 16.00
Breed: Purebreed Nordanner
Personality: Rage is the embodiment of confidence; sometimes he may even appear a bit too confident, speak: arrogant. Besides this trait he enjoys fun and hanging around with his equine buddies. He is loyal and respectful to those who show him respect, and thus the perfect partner once you bond with him. He is a perfectionist; everything he does – even if it is playing a prank on the people around him – is planned out well. He doesn´t like losing, and he will always do his best in the show ring (if he doesn´t you can be pretty sure there´s something wrong with him). He can be very gentle and loving around beings he cares for, but also very protective, and even dangerous when infuriated.
Likes: Attention! Besides that: going on adventures when he´s in the mood, but he also likes quiet moments where he can be by himself. Everything he considers beautiful; for instance he loves to watch the Northern Lights if he gets the chance to.
Quirks/Dislikes: He might be hot-headed when he´s angry (usually he thinks before he acts). Has a tendency to get jealous; he dislikes it when you openly favour someone else. Is not overly fond of thunderstorms for whatever reason. Doesn´t like to be left all alone for too long.
------------------------------------------ SSS: 231 Little Timmy (starter)
----------------- SS: 5726 WF's As You Like It
------------------------------------------ SSD: 514 Goldie (starter)
Sire: 7463 WitchDoctor's Gentleman
------------------------------------------ SDS: 1280 CDS Atlantis' Downfall
----------------- SD: 3523 MFS Dark Moon Gold
------------------------------------------ SDD: 2561 Siberia
------------------------------------------ DSS: Smeltet is 1379
----------------- DS: JK's Hand That Strikes 4765
------------------------------------------ DSD: Dovahkiin #885
Dam: 7720 Mystic Stranger
------------------------------------------ DDS: Rangoon 4645
----------------- DD: Bjork 5313
------------------------------------------ DDD: Golden Girl 509 (starter)
Continued from the sire line:
SDSS: 113 Dane's Expresso (starter)
SDSD: 477 Heart of Atlantis (starter)
SDDS: 1372 DDS Ohzaxuzief
SDDSS: 460 A Coming Exodus (starter)
SDDSD: 132 EWS Shiramasu (starter)
SDDD: 901 Nameless Liberty (starter)
Continued from dam line:
From Smeltet is 1379 :
DSSS: Natfjer 1267
DSSSS: Daimhin 476 (starter) :
DSSSD: Femme Fatel 840
DSSSDS: Arina 474 Deceased (starter)
DSSSDD: Shiya 98 Deceased (starter)
DSSD: Ice Cream Sundae 1338
DSSDS: Eximius #27 (starter)
DSSDD: Aithne 850
DSSDDS: Rhyfedd 120 Deceased (starter)
DSSDDD: Ulmende 149 Deceased (starter)
From Dovahkiin 885
DSDS: Majeed 126 (starter)
DSDD: Eowyn 11 (starter)
From Rangoon 4645
DDSD: AS Amontilado 965 (starter)
DDSS: Flower Power 3268
DDSSS: I know Kung Fu 147 (starter)
DDSSD: Desert Orchid 2537 Deceased
DDSSDS: Draco 1659
DDSSDD: ArDes Desert Royalty 506 (starter)
DDSSDSS: DHF's Apocalypse 454 (starter)
DDSSDSD: KEC Maybe Means No 1260
DDSSDSDS: BR Silverlight 193 (starter)
DDSSDSDD: KEC Road to Mordor 395
DDSSDSDDS: AS Hey Arnold 424 (starter)
DDSSDSDDD: SKG Panthera 119 (starter)
Related to the following Peafowl starters
Ulmende 149 Deceased
Shiya 98 Deceased
Related to the following Laced Tobiano starters
Related to the following Panda starters
Related to the following Fawn starters:
Shiya 98 Deceased
Related to the following Bloodsplash starters
Shiya 98 Deceased
Related to the following Atlanticus starters
Related to the following natural starters:
Rhyfedd 120 Deceased
Arina 474 Deceased
Breedings: NONE. Gelded.
I talked to both Heca-Bitch4Life (the sire owner) and Astralseed (the dam owner), and they are okay with him being gelded.
We agreed that it might be nice to have a long lineage on a mutation horse, but that it would be equally insane to breed with a horse with such a monster of a lineage.
Accepted Foal/Import Design:
Breeding note: nordanner.deviantart.com/admin…
Both Daily Tack and Dragon Hunter Tack
Ancestors with "Quality Blood" status: 24
- 231 Little Timmy
- 113 Dane's Expresso
- 460 A Coming Exodus
- Eximius 127
- Rhyfedd 120
- Eowyn 11
- KEC Road to Mordor 395
- DHF's Apocalypse 454
- 1280 CDS Atlantis' Downfall
- 1372 DDS Ohzaxuzief
- Ice Cream Sundae 1338
- 2561 Siberia
Ancestors with "Excellent Blood" status: 67
- 477 Heart of Atlantis
- Golden Girl 509
- 132 EWS Shiramasu
- Majeed 126
- SKG Panthera 119
- Shiya 98
- AS Hey Arnold 424
-ArDes Desert Royalty 506
- I know Kung Fu 147
- Arina 474
- Daimhin 476
- Dovahkiin 885
- 901 Nameless Liberty
- Femme Fatel 840
- Aithne 850
- AS Amontilado 965
- Natfjer 1267
- Flower Power 3268
- Rangoon 4645
Ancestors with "Supreme Blood" status
-BR Silverlight 193
Kaaring through Pedigree = 95
One registration, colored, headshot or full body:
1 point
Uncolored art (0.5 points per picture) :
Partial body, colored (0.5 points per picture) :
Full body, colored, no or plain backgrounds (1 point per picture) :
Full body, colored, painted backgrounds (2 points per picture) :
Show entries OR training images OR Dragon tack images: +1 point (not stackable) :
Show Placings:
Animations (+2 points) :
→ 3 points so far
The Levels
95/15 15 points
95/30 30 points
95/45 45 points
95/60 60 points
95/75 75 points
95/200 points
1/400 points
Kaaring through Pedigree = 95 + 3 picture points = 98
Reference by EquineStockImagery
Design by SweetLittleVampire (me)
I think I would have died while researching it all. XD
Thank you. :3
You can never have enough Icos. XD
I could grow his hair out, but I think with the markings in his hair this looks better.
Also he´s not entirely based off Loki, but I wanted to use green and gold (the blue version was only a variation of it), and with the whole movie craze the association came automatically.
Oh, he can be pretty nasty too!
black and green is an especial color combo favorite of mine >.>
And you and your friend deserve a trophy for figuring out everything associated with that lineage... You deserve five trophies. O.o four small ones and one huge one with the entire lineage engraved on the front
A reason why I gelded him; first of all I´m not that sadistic and want anyone to have to reproduce that monstrosity of a lineage; and secondly he´s related to so many starters...breeding him will be difficult.
Except you want a black natural Nordanner, and there are easier ways to get one.
But thank you! It depends on the lacing for me. Some I like, some I don´t.
'Vexation And Rage' 'Trust That I Want To End You' 'A Linear Account of Rage' Oh the possibilities.
I have to agree with you on the lacing, I just saw some other lacing that I really liked, so I think what happened is the few lacing examples i'd seen just didn't click with me for some reason. It can be reaaaally beautiful, as your guy shows so well.
And now I just had this inspiring idea of drawing him next to the big trophy, and having the trophy and lineage continue on into infinity in the sky. XD I'm sure it must have felt never-ending when you were doing it, lol.
Oh, and I though by choosing a Loki quote as name I might spark a wave of movie quotes as Nordanner names. XD
I loooove DemiWolfe-Stables´s lacing style; it reminds me of Art Nouveau.
Just look at this one: fav.me/d5ze3ly
She has many more gorgeous designs in her gallery.
XDD Sound like something one could do with a chibi.
That would be awesome though, movie quotes make very dynamic names.
Ooo those are lovely! I think this one is my especial favorite:
Looks like he has a fold of fabric coiled over his bum
I just happen to be a Loki fangirl, and gold and green are the colours he wears in the movies, so I thought one of his quotes would fit as a name.
Hehe, never looked at that particular lacing that wa, but you´re right.
Gold and green are his colors huh? That's awesome.
Well I've always been enamored with beautiful folds of fabric (Renaissance era paintings with all dat gorgeous patterned fabric...*meltmelt*) so that could be why it looks like that to me. XD
It´s a fun movie, though you may have to watch "Thor" first to understand why Loki does what he does in "Avengers".
Ooooh, Renaissance paintings!
"It is Known"
"Run Forest, Run!"
"Hail to the King baby"
"This is my boomstick!"
"Listen up you primative screwheads"
and from loony toons
"Son-of-a..bedip son-of-a..bedip.. son-of-a..bedip.. gun"
"You thought I was going to say..bedip.., son of a bitch, didntcha?"
"Whatsup Doc?"
"I tawt I taw a puddy tat" (..might have to name a tabby or other cat horse that lol)
Game quote:
"How might these abominations be manipulated?"
schade, dass es ein hengst ist, sonst hätte ich nach einen slot gefragt (hab nur hengste) xD
Würde jetzt eh nicht mehr gehen, ich hab ihn gerade kastriert. XD
Mit dem Stammbaum findest du kaum noch Pferde, mit denen er nicht verwandt ist.
Ich besitze nur eine Stute.
darf ich die stute mal sehen?
ich hab nen silver seal bay hengst
mein grullo muss noch registriert werden
(kann leider nicht mit punkten dienen, die brauch ich als preise fürs Japanese Cherry Blossom Event)
Normalerweise nehme ich noch ein weiteres Bild von meinem Laced Hengst (den ich dir verlinkt hatte) mit dazu, damit ich ihn auf 200 Kaaring Punkte kriege und wieder zur Zucht öffnen kann; das braucht aber dann auch nur ein unschattiertes Kopfbild zu sein oder so.
Wäre das in Ordnung für dich?
breeding pic ist bei mir sowieso pflicht
und der headshot geht auch klar
hab das cell shading gerade für mich entdeckt, währe das i.O. für dich?
die pics werden ne weile dauern, da ich heute meine Schwiegergroßvater (nennt man das so?) besuche
und ich hab noch ein HWB Breeding pic auf der liste, mit foal design, das hat vorrang, da es schon länger ansteht
To be honest, I´m thinking about gelding him, but I´m not sure yet. With that lineage it´ll be near to impossible to breed a peafowl or laced with him, as he´s related to so many starters...
And there are way easier ways to breed a natural black Nordanner if you want one.
That lineage was painful to do for me and for the lady who helped me.
So I have a bit of time to think it all over.
You do have a valid point there, though.
And no need to rush to a decision, right? Not like he's going anywhere
Here's 132's new link: fav.me/d7b009g
1338's new link: fav.me/d7bhsnv
193's link: fav.me/d5rll6i
Donno if anymore are out of date lol
I was so happy to see this beautiful baby go through! He's so pretty <3
Thank you so much!
You're welcome
I demand you draw all my references cause wgiawehgieuwghawui yours are so pretty
Hehe, for whatever reason I don´t use my "usual" reference sheet for my Nordanner.
I kind of like that one though; wished I had used that background for my two other Nordies.
Hire me. Could be expensive, though. XD


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The craftsmen at Nagoya are at it again. They bring us another absolutely splendid set of 5 bowls, each with its unique hand-printed design. The art runs the gamut from abstract lines to a more patterned look, featuring their artistic favorite subject, flowers! There is liberal use of the color blue, and occasional bits of red and green enliven the senses. A set to be treasured.
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SUPERMAN WONDERWONAN by Sandoval-ArtWatch
©2014-2016 Sandoval-Art
Done with mechanical pencils 0.03 HB
I love your style dude
OH wow! This is so awesome! PLEASE upload the high res! I'd love to ink and color it!
this is amazing you should be a comic artist youre fucking amazing! i love their body language and facial expressions, this drawing tells a story. i think youre an amazing artist. 5 out of 5.
Amazing I love it!
Still... Speechless
Holy Heck! This could easily be an official cover!
Every time you draw Supes and WW, I just love it. Awesome work, as usual!
Nicely done
Oh wow!!! The sheer joy and the layout in this is AMAZING!!!!!
Your Wonder Woman is beautiful! This is a very nice drawing (of both heroes).


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Unified Messaging
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Three consecutive Sundays of Nathaniel Dorsky's resplendent films at the Pacific Film Archive
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Top picks from San Francisco Cinematheque's third 'Crossroads' festival
FILM San Francisco Cinematheque artistic director Steve Polta balances familiar names with lesser known for the third annual "Crossroads" festival at the Victoria Theater, though Ken Jacobs' Occupy-strength Seeking the Monkey King (2011) promises to unseat the image of a mellowing old master.Read more »
SUMMER MOVIE PREVIEW: Movie stars, superheroes, and movie stars playing superheroes battle for summer supremacy
FILM So far, 2012 has been a year of mixed blessings for Hollywood, contrasting mega-hits like The Hunger Games and The Avengers with one of the biggest mega-flops of all time, John Carter. But summer's really when show-biz turns deadly serious. Each week, there's a new wannabe blockbuster — pasteurized, processed, film-like products so huge they have the ability to make or break entire movie studios — hoping for returns big enough to make all involved even richer, and insure sequels and spin-offs for summers to come.Read more »
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17 Springful Easter Side Dishes
Freshly squeezed orange juice and zest gives this cake an intense flavor. You'll love the sweet-tart cream cheese glaze.
Recipe: Daffodil Cake
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Recipe: Strawberry Cream Cake
Springtime Dessert Recipes for a Mother's Day Brunch


text: 125 FGUS65 KSTR 032039 ESGCO NATIONAL WEATHER SERVICE COLORADO BASIN RIVER FORECAST CENTER SALT LAKE CITY, UT SPRING FLOOD POTENTIAL FOR THE COLORADO RIVER BASIN WITHIN COLORADO FEBRUARY 3, 2016 The 2016 spring runoff flood potential is considered slightly higher than average in the Dolores and San Juan basins at this time. The current above normal snowpack combined with a strong El Nino event suggests an elevated potential over that of a normal year. The 2016 spring runoff flood potential due to snowmelt is near normal at this time for the Yampa/White, Upper Colorado mainstem, and Gunnison basins. It should be emphasized that it is still early in the snow accumulation season and conditions could change quite a bit before the runoff begins. January basin averaged precipitation was generally near to above average across western Colorado with values between 105 and 120 percent of average. However, the precipitation pattern was variable with below average precipitation occurring in the eastern portions of the San Juan, Gunnison, and Colorado River headwaters while the western portions of those basins, as well as the Yampa and Dolores River basins had above average precipitation. Seasonal precipitation is near to above average and ranges from 100 percent of average in the Yampa/White basin to 145 percent of average in the Dolores River basin. February 1st basin averaged snow water equivalent is 135 percent of median in the Dolores River Basin with some individual SNOTEL sites over 150 percent of median. The rest of the basins in western Colorado have basin averaged snow water equivalent between 100 and 115 percent of median as of February 1. The current volume forecasts for the April through July runoff period range between 85 and 110 percent of average in the Yampa/White, Gunnison, San Juan, and Upper Colorado mainstem basins and between 115 and 125 percent of average in the Dolores River Basin. Although spring temperatures affect the pattern of snowmelt runoff and consequently the magnitude of peak flows, peak flows also roughly correspond to volumetric flows. It is also important to keep in mind that an extended period of much above normal temperatures or heavy rainfall during the melt period can cause or exacerbate flooding problems in any year. CBRFC/Alcorn


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Fort Leonard Wood celebrates Hispanic American Heritage Month
October 4, 2012
By Ms. Martha Yoshida (Leonard Wood)
FORT LEONARD WOOD, Mo. - Hispanic American Heritage Month is under way and to help celebrate the contributions of Hispanic Americans, Fort Leonard Wood held its Hispanic American Heritage Month observance and luncheon Wednesday at the Pershing Community Center.
The event, hosted by the 3rd Chemical Brigade featured Yolanda Lorge, Grupo Latinoamericano Dance Company president, as the guest speaker.
According to the Library of Congress website, each year, Americans observe National Hispanic Heritage Month from Sept. 15 to Oct. 15, by celebrating the histories, cultures and contributions of American citizens whose ancestors came from Spain, Mexico, the Caribbean, and Central and South America.


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Are you Thanh X Dang?Claim your profile
Publications (3)9.69 Total impact
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ABSTRACT: To facilitate purification and subsequent structural studies of recombinant proteins the most widely used genetically encoded tag is the histidine tag (His-tag) which specifically binds to N-nitrilotriacetic-acid-chelated nickel ions. Lipids derivatized with a nickel-chelating head group can be mixed with galactosylceramide glycolipids to prepare lipid nanotubes that bind His-tagged proteins. In this study, we use His-tagged perfringolysin O (PFO), a soluble toxin secreted by the bacterial pathogen Clostridium perfringens, as a model protein to test the utility of nickel-lipid nanotubes as a tool for structural studies of His-tagged proteins. PFO is a member of the cholesterol dependent cytolysin family (CDC) of oligomerizing, pore-forming toxins found in a variety of Gram-positive bacterial pathogens. CDC pores have been difficult to study by X-ray crystallography because they are membrane associated and vary in size. We demonstrate that both a wild-type and a mutant form of PFO form helical arrays on nickel-lipid containing nanotubes. Cryo-electron microscopy and image analysis of the helical arrays were used to reconstruct a 3D density map of wild-type PFO. This study suggests that the use of nickel-lipid nanotubes may offer a general approach for structural studies of recombinant proteins and may provide insights into the molecular interactions of proteins that have a natural affinity for a membrane surface.
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ABSTRACT: In this study, we use streptavidin (SA) as a model system to study helical protein array formation on lipid nanotubes, an alternative to 2D studies on lipid monolayers. We demonstrate that wild-type and a mutant form of SA form helical arrays on biotinylated lipid nanotubes. 3D maps from helical arrays of wild-type and mutant SA were reconstructed using two different approaches: Fourier-Bessel methods and an iterative single particle algorithm. The maps show that wild-type and mutant streptavidin molecules order differently. The molecular packing arrangements of SA on the surface of the lipid nanotubes differ from previously reported lattice packing of SA on biotinylated monolayers. Helical crystallization on lipid nanotubes presents an alternative platform to explore fundamentals of protein ordering, intermolecular protein interaction and phase behavior. We demonstrate that lipid nanotubes offer a robust and reproducible substrate for forming helical protein arrays which present a means for studying protein structure and structure-function relationships.
Article: Prepore to pore transition of a cholesterol-dependent cytolysin visualized by electron microscopy. J Struct Biol[Show abstract] [Hide abstract]
ABSTRACT: Perfringolysin O (PFO), a soluble toxin secreted by the pathogenic Clostridium perfringens, forms large homo-oligomeric pore complexes comprising up to 50 PFO molecules in cholesterol-containing membranes. In this study, electron microscopy (EM) and single-particle image analysis were used to reconstruct two-dimensional (2D) projection maps from images of oligomeric PFO prepore and pore complexes formed on cholesterol-rich lipid layers. The projection maps are characterized by an outer and an inner ring of density peaks. The outer rings of the prepore and pore complexes are very similar; however, the protein densities that make up the inner ring of the pore complex are more intense and discretely resolved than they are for the prepore complex. The change in inner-ring protein density is consistent with a mechanism in which the monomers within the prepore complex make a transition from a partially disordered state to a more ordered transmembrane beta-barrel in the pore complex. Finally, the orientation of the monomers within the oligomeric complexes was determined by visualization of streptavidin (SA) molecules bound to biotinylated cysteine-substituted residues predicted to face either the inner or outer surface of the oligomeric pore complex. This study provides an unprecedented view of the conversion of the PFO prepore to pore complex.
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The Scripps Research Institute
La Jolla, California, United States
- Department of Cell and Molecular Biology


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Hey, I'm Daniela!
Hi! I was born in Transilvania, I studied German and Italian language and literature at the Bucharest University (during the period of the revolution) and I came to Berlin with a stipend from the Humboldt Universität in 1991. After one semester in Berlin I could not imagine anymore to leave this city. Anyhow, Berlin was indirectly my hometown since my childhood because my granny lived here in the 20-ies and she used to tell me a lot things about her youth in Berlin...
Since one year I am running a café, the Amarcord in Friedenau. Many guests visit me here, especially for music events.
I love travelling - all kinds of travelling - everything but club-holidays in a fix hotel. The most exciting holidays I had till now where 2001 - 1 month by my one in Rio, 2005 - 1 month travelling by bus with my boyfriend through Mexico and 2008 travelling with my boyfriend by car through Costa Rica. In Europe I love most Sicily and Andalusia.
I love diving and skiing, books and movies, my parents and my friends, good food and wine.
My favorite books are: "When Nietzsche wept" - Irvin D. Yalom, "Và dove ti porta il cuore" - Susanna Tamaro, "De ce iubim femeile" - Mircea Cartarescu, "Briefe in die chinesische Vergangenheit" - Herbert Rosendorfer, "Die Glut" - Sándor Márai & a lot more
My favorite movies: My life whitout me, The bridges of Madison County, Il Ciclone, Benvenuti al Sud, Non ci resta che piangere, Cinema Paradiso
Favorite music: Ligabue, Quadro Nuevo, Maná, Eva Cassidy, Marc Anthony
Favorite food: caponata, taglioglini al tartufo, Spinat mit Spiegelei, salata de vinete, chicharrones de chiclana, bolinho de bacalao...
Languages I speak: German, Italian, Romanian, English, Siebenbürgisch Sächsisch (an old funny German dialect) and Spanish (very very bad Spanish, hope I will improve my Spanish with my guests)


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