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Marriage Visa Green Card USCIS Interview Questions By Immigration Lawyer Mark C Daly
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Did this video help you?
Rigo Herrera: Hi Mark, My Wife which is a U.S Citizen Is filling For me a I-130 I am Now not in the U.S Since 2012 I been in the U.S since 2007-2012 & we have a son together & another on its way How long would this process think it should take To get approved?
Óscar Balbuena: Hi my name is Oscar, I have case going on... The interview for my wife and my daughter will be on Tuesday the 14th, we have done the physical exams, but.... my daughter got "bad results'' on the physical, they said that she had been exposed to have Tuberculosis and we want to know if she will get the visa or they have to reschedule it? She is a very healthy girl (she is 3 years old) I'm very worried about it. i hope you answer. thank you and have a blessed day.
raquel a: Thank you. Very helpful. :)
Tarik Haroon: i have one step the money i dont have visa card on my contrey what can i do to bay a card plz help me
Yanina Hernandez: Hello Mark,I was married years ago and I filed for the Cuban adjustment with my ex husband. Things went really bad,we were nervous and they ended up denying my residence.After few years married and we decided to get a divorce. In the meantime I met my husband who im currently married to and we filed already.We are waiting for an interview date. What is the best thing to do in this case since my last case was pretty bad? Should I take a lawyer with me to my next interview?
reven jarog: Hi Mark, I would like to ask that can my wife take our daughter 1 an half (infant) with her to interview in embassy in Islamabad Pakistan.? i am us citizen so my daughter is already citizen however we still have to report birth abroad in embassy and probably my wife will do it after her interview.
Kareli Leyva: I would really like to know all the money that goes into all the forms and things needed to be done for the Marriage Visa. Please,
Mark Daly: I get where you are coming from. My job is to advise people of all of the options under the law, and my experience is that people start to discover support and assets that they did not consider when I give them the overview of what is possible under the law. And I agree, it does seem that there is no family support in this case! M
btigress1: Maybe i'm wrong but I naturally assumed that someone who needed such a long time to save up enough money just to apply for the I-130 had little to no family support. I also can only imagine that someone like this, has no assets to put forth for their Affidavit of Support, maybe I assume too much though.
Mark Daly: Hey btigress1 I have to disagree....there are many ways to get the visa if you have made enough money to support 2 persons during the previous year!!! Most of my clients will get a joint sponsor to complete an I-864, and others will use assets to qualify. However I do agree that if you can get the I-130 filed now you will not have any other major expenses until the case is approved and transferred to the National Visa Center.
btigress1: My husband has his immigrant interview in one month.
btigress1: I would recommend that as long as you will have the remainder of the money needed to complete the process within 5 months, go ahead. We heard nothing back from USCIS for 5-6 months then once the I-130 is approved the ball gets moving and you need to have the funds to finish up. In addition you will be asked for the affidavit of support and your tax return for 2012. If you didn't make enough money to support 2 persons during the previous year, don't bother starting the process. Good luck!
Rosa MM: Its very hard to find a good Lawyer, with a good heart.... I wish i can get the help i need from somebody like u, but i am in california, any ways i dont want to be deported because i am mexican and y been living here ilegally and there is no chance at this time for us to adjust our status, even married with a citizen... Thanks for all your videos
hopeton gr: thank u mark daley your the best u help me all the way through to my green card thank u again
cigs41: my interview is tomorrow and i really believe this video will help.... im still nervous though
nakach ahmed: i need fast visa , my baby will born soon i have to be there, what i have to do
Mark Daly: @drmannanazam NICE GOING! I am so glad that you were able to use this information to help you. It is so hard to get a lawyer on the phone and get a couple of questions answered. We are going to be making more videos available on more forms and information - and opening up more offices around the country. It is exciting to spread good vibes in a business polluted with smugglers, notarios and bad attorneys!
Abdul Mannan Mohammed: wow this clip is highly helpful. i had my green card interview through marriage yesterday and it was wonderful. I followed what ever this video says. thanks Mark u are a superb lawyer. what all question you narated here 80%was asked me. than you very much.
gracejorge26: @Bunchhieng what the consul ask to your mom,if you dont mind and how many month she process the application?
Bunchhieng Soth: Yeah she passed!! Thank!!
Marriage Visa Green Card USCIS Interview Questions by Immigration Lawyer Mark C Daly 5 out of 5


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Respond to Review of Sakonis - Harrow
by dinitro
Review: I'm writing this after I submitted a review about Sakonis in Wembley. The buffet range is much better here than Wembley, parking is also easier. However the menu still suffers from over salted food and greasy food. Apart from these complaints the food is reasonably priced and very tasty. I have visited Sakonis over a number of years and it has always been a favourite with the kids and the whole of the family. When it first started in was novel, fresh, highly competitive, the cleanliness of the decor, the fast service. They have lost their way a little, however new diners will still appreciate this non pretentious restaurant. Service is better than Wembley.
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Insider Selling Hits All Time Record Of $4.5 Billion In Prior Week As Everyone Is Getting Out Of Market
Insiders have officially marked the top of the stock market: last week's insider selling of all stocks (not just S&P) hit an all time record of $4.5 billion. This is the biggest weekly number ever recorded by tracking company InsiderScore.com: as Sentiment Trader highlights no other week before had more than $2 billion in net selling. Furthermore, selling in just S&P companies hit a whopping $2.8 billion: over 4 times more than the week prior! As such the ratio of insider selling to buying is now meaningless. Even Bloomberg, which traditionally just posts the data without providing commentary to it, highlighted this ridiculous outlier: "Insider selling at Standard & Poor's 500 Index companies reached a
record in the past week as executives took advantage of a two-year high
in the stock-market to sell their shares." We hope those retail investors who dared to reemerge in the stock market and play some hot potatoes with the big boys, enjoy their brief profit as they once again end up being the biggest fools.
Here is a stunning chart of insider trading activity, courtesy of SentimentTrader:
And this is what insider buying to selling in the S&P looked like last week. LOL.
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So much for the Bernanke thesis of the "wealth effect".
On the flip side of all this selling, these guys just got dollars instead of stocks... lets see how that works out.
I just hope they don't move into Silver, I NEED a pull back!
The market usually tanks when everyone thinks things will go on forever. This time it is indeed different. Everyone has had 2 years to get ready for our impending economic collapse and no one wants to a bath.
Yeah, I would like a pullback as well... Nevertheless, I'll be buying silver even at $100... it's MONEY, not currency.
Can you blame them for selling? They know that the FED is pumping up their stock price and it's not worth half as much as it is now. They are locking in profits. The suckers are buying. Same game, different name.
The wealth effect is even easier to control because there is no profit motive in the stock market anymore. People selling now are locking in gains. The rest of the ownership is via the government through POMO bank cash trade in money. Banks are specifically told not to sell stock if they participated in the Pomo trade in. They were able to trade toxic poo for bonds and then cash to gun the stock market.
Even a declining equity is greater than any toxic asset or derivative.
There is no real motive to sell until the FED gets audited or a threat to bank bonuses. Then the banks rebel, and flash crash the whole thing.
It is easy to control the stock market now- the bond market which is 80x bigger is harder to control- that's the big problem for Benny and the ink jets.
go to now ye rich men, weep and howl for your miseries that shall come upon you.your riches are corrupted,you have lived in pleasure and been wanton. this day a grievous sore of poverty hath been left on the doorstep of AMERICA,thou art declared to be ICHABOD.
behold a pale horse
You wrote--
>>>It is easy to control the stock market now- the bond market which is 80x bigger is harder to control- that's the big problem for Benny and the ink jets.<<<
Not so. Only about twice as big.
This is important to understanding how things may come unraveled and their relative importance.
Do your homework. Don't shoot from the hip.
he meant the "poor health effect."
Oh, I think the Bernanke "wealth effect" is doing exactly what it was intended to do - support the "plutonomy" and allow the Playerz to get out with as much liquid cash as they can prior to the coming, uh, realignment in market values.
"Realingment" in market values. I like that. That will occur after the "economic earthquake" with the catalyst being periphery defaults... T minus 4 days and counting.
This surprises me. I didn't think there was anybody left.
You thought executives had stopped granting eachother oversized option grants?
Exactly, Most people have not figured out that with the size of these options grants to upper management, the companies profits will never have anything left for shareholders. In a lot of cases, the companies future profits are distributed to the bonus pool leaving no hope for shareholder increases. This is why stocks have not appreciated over the last decade. The money was already stolen right under your nose. And all they pipe about is shareholder value. LOL
Right you are. If you want to own equities, own them in Australia or Canada. Not to say that from a growth perspective they will perform better than US equities (although they probably will) but the dividend yields are for real. Profits ACTUALLY get distributed to shareholders, not just management. Cashflow actually exists in these countries.
no worries! POMO is coming up tomorrow!!!!!!
embrace "changes" =)
Jeremy Grantham video to be aired at 4:30 PM today (29 minutes)
[Video] CNBC - Jeremy Grantham Speaks
"The Fed has spent most of the last 15, 20 years manipulating the stock market, whenever they feel the economy needs a bit of a kick," he added. "I think they know very well that what they do has no direct affect."
Brian Sack to the rescue!!
Good luck.
Today is a good day to die....or flash crash without the dead cat bounce
But the Zerohedge bulls insist the place to be is IN the market waiting for DOW 36,000, so that a cash-out can happen when the US $LOLlar is -0-. I never thought it was a good plan myself, I feel much better off with the PM's, bullets, beans, etc.
Wine is a good pick too
Take delivery !!
funny. they could still be right, but funny.
Perhaps all the insider trading is coordinated. The PDs have the insiders sell part of their holdings. A pat on the back for a job well done for the banksters. "Take your profits, boys. And don't forget to cut yourself a righteous bonus as well." That spooks markets so PDs can buy lower when QE ass activity restarts. Except, I think there's a fly in their soup.
'QE ass activity'
I think it all centers around that indeed!
Ok so we know who is selling (company insiders) and how much (a whole shit-wack) but who exactly is buying? What sort of alchemy is at work here for such "robust" selling to be met with new highs? I thought all the mutual funds were at record low cash levels? I know this is crazy but why do I get the feeling government is the buyer and the bond market is just starting to catch on?
If the bond market guys are only now starting to catch on that the FED is the only buyer, then we are well and truely fuked!
They are selling to buy more NFLX and LVLT...look at those stocks go since open. This is going to be spectacular now that the seams are tearing.
Ballmer dumped $1 billion worth of Microsoft Dummies. That's the reason. No reason to report facts when Hype works so much better. Buy Gold Lemmings. LOL
4.5 billion - 1 billion is 3.5 billion. Still a ridiculous number compared to insider buying transactions.
How did you beat the math question to post.
Bad read dude- even without Balmer it was $3.6B - still a six year high, but why do diligence when being flippant works so much better.
You're oh so correct. If you subtract the billion pulled out of Microsoft, it's only a record sell off by 1.5 billion. Pure hype. Move along.
What are 'gold lemmings' and where can they be purchased?
And dont look now, PM punchers, but gold and silver are again deep in the green. Damn Harry must be coughin up blood by now.
so who in the holy hell is buying? Insiders are selling, mutual funds are outflowing...where is the mkt support coming from?
I think this only leaves the Fed...
flash crash day showed us that there are NO size buyers even 10% below last...
Lots of buyers... the PDs, the PPT, the... er, did I mention the PDs?
Trav. Stop asking questions. They only serve to bring into question the effectiveness of the recovery.
MSFT accounts for most of the sales, of which Balmer is responsible for nearly all of it. Balmer sold $1.3bn of MSFT for 'tax planning' purposes.
Even still, take him out as an outlier, and total sales are still 50% higher than the next highest weeks...
they flee stocks and put it in bonds - fueling yet another bubble. Is is time for TBT?
Add to my gloom omen list...
a. Harbinger...dying on the vine....
b. The luck of the Irish finally runs out...
c. Rats seen jumping ship....
d. ...(still early days yet!!!)
I need a moat around my castle . . .
Pretty much playing out like I said after CSCO last night. Big drop at the open for stocks then rally throughout the day to end green. Easy as it gets.
Highest rate of insider selling in HISTORY... And Harry is buying.
Harry must be Geithner. You pukin up blood yet man?
The FED IS BUYING. Until they don't we go higher on small dips.
FED buying with their imaginary dollars, with no one in the world dumb enough to dump to...WOW Harry please tell me how that works out!
To repeat from last night:
Bottom line, this "bullshit" can go on for years and years. Yes, it'll end in tears but you can keep this running for a couple more decades easily. With that comes rising equity prices. It's a clown show. They chase in a circle dancing wildly until the music stops. China has to appease the US. The US has to appease China. And no one gives a flying fuck about the rest of the world. This can go on for a very, very long time. And it will. Glad to know it probably won't happen in my life time but sucks for those who have kids.
Bottom line, this "bullshit" can go on for years and years. Yes, it'll end in tears but you can keep this running for a couple more decades easily. With that comes rising equity prices. It's a clown show. They chase in a circle dancing wildly until the music stops.
China has to appease the US. The US has to appease China. And no one gives a flying fuck about the rest of the world. This can go on for a very, very long time. And it will. Glad to know it probably won't happen in my life time but sucks for those who have kids.
'Can go on for years and years'...LOL dream on!
for "Decades"?? Why?
Because we're just QE-ing like Japan? Because we're a nation of fish eating salarymen savers and an exporting juggernaut with but a tiny military and public sector riding high in a world of easy credit and conspicuous bubble consumption??
Put down the pipe and step AWAY from the bubbles ooo0000
Rising commodity prices will kill this clown show. Denninger had a good piece on his site about margin compression (including Campbells soup of all things) last night that I thought was spot on. If food prices keep going north in a big way....things will get dire quickly:) Hope you own farmland
Man, oh man. You're saying Ballmer sold out a 1 billion position in MSFT so he could stock up on canned soup? The future is gonna be worse than I thought. May have to roll the AU over into good whiskey.
It should be obvious by now, the FED has two options: monetize the defecit or the entire federal complex goes bust.
It's either inflation or total default. Which would you choose? (Hint, the sheeple get more pissed when they can't even buy campbell's soup, vs. spending more and still having it on the shelf)
i can't distinquish any correlation in that chart except for the time they had massive buying volume spike where the market tanked shortly afterwards. other than that it looks like flipping a coin.
Mish highlights the insider selling at Cisco.
I'm not waiting for any SEC investigations...
All that selling and still a negative effect on the VIX, which closed down significantly on 11/5 (18.26) versus prior week ending 10/29 (21.25). So.....that tells me that the Bear-hankie put is in full force, we have clear support in the market, and insiders are profit taking coming off of earnings. Even they are saying the markets are so fraudulent that they would rather have their money somewhere else than their own company stock. Golly jee wiz Beaver, it looks like they're pouring money into commodities (including PM's of course). PS I'm new to this, trying desperately to hang onto what wealth I have left, and learning quickly... so I politely ask that criticism to be constructive and educational. Any kudos would add a warm fuzzy layer to the inside of my bag....hint hint prod prod...-
Wonder if everyone should take that as hint ala Cisco which had 6 months of insider selling and no buying before their announcement of things not so peachy. Guess things aren't going to be puppydogs and rainbows elsewhere.
...buy! buy! buy!....wait...sell! sell! sell!...wait..um...
...perhaps the new coke will be sucker punch flavor..."K"nocked "O"ut.
We used to call this an exit strategy. In order to exit, however, you needed volume and buyers. That this is happening with FRB action is disconcerting to say the least.
Insiders selling is normal. They get stock options and the only way to make money from that is to exercise, get the stock and sell it. Without adjusting data for >>SCHEDULED<< option compensation this is nothing more than sensational journalism.
"Oh look! A convenient distraction."
The insider selling is what the POMOs are for, to provide the liquidity drained by the rapacious corporate executives. There has to be money for the corporate stock buybacks.
While it may be truth that insider selling is at "record levels", this should not be considered "bearish", but rather, just more of the same "money printing" and "wealth transfer". Most likely corporations have concluded that if the government can continuously "debase" its currency by printing money, they can too.
Bottom line is I have studied the markets for decades, and find it interesting that nobody has EVER been concerned about the money printing (stock printing aka wealth transfer) of corporations. They have always done it; however, my guess is they are just stepping up the pace now that they see the government can get away it with no real consequence. Add to this the fact that many executives likely want to purchase more real assets to diversify away from the Ponzi fiat dollar, and all you are really seeing is just a confluence of factors leading to more money printing (via stock options) by corporations.
Nothing to see here except widespread acceptance that fiat money printing is now socially and politically acceptable and no longer considered taboo. Moreover, corporations realize that with the Bernanke "put", they can debase their stock at an accelerated rate without worrying about its immediate effect on their stock price; afterall, fundamentals like PE, earnings, etc., no longer matter, and even if they did, corporations are not required to value their balance sheets at fair value or report honest earnings anyway, so no worries.
At the end of the day, all this insider selling (money printing via stock options) really could be considered bullish----it means corporations feel right now is a good time to "monetize" their fiat currency (stock) in the market without fear of it hurting their stock price (and thus future money printing) too severely.
Hope this helps anybody thinking that this entire insider selling is bearish----it has been this way for decades---it is simply happening more now that corporations realize that there are no penalities in America for immoral, criminal, or corrupt behavior; provided you know the right people, illegal behavior is actually rewarded. In other words, when you combine the fact that the government has the stock markets back with the Bernanke put, and, most investors in the market are fools anyway and don't understand how corporations "print" money via stock options for their employees, what is there to worry about?----just print, print, print. As long as the public is willing to buy "fake" money via the corrupt stock market, corporations will be more than happy to sell you the paper----and like Benny and the Jets, they can print all day and night----so no worries, just more of the same only at an accelerated rate. If and when (never) the public learns how to balance their own checkbooks, then you might need to be worried----in the meantime, business as usual.
Arent these same Insiders the people making the decisions to issue debt to fund massive stock buybacks and dividends, both of which are aimed at pushing the stock price higher? Isnt this somewhat shady? Basically pillaging the companies balance sheet so they can sell their own personal stock at higher prices??
Stockholders are not acting out, so it must be OK! :D
Why bother paying dividends when you can have it all yourself without the actual owners saying a peep, or even comprehending what's happening.
They even made comment about the insider selling on CNBC, and the market has been down a few days. I think they know the plug is about to be pulled and they are running like crazy.
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FLOWERY BRANCH - When Michael Turner arrived at training camp a year ago, he had to put all doubts out of his mind.
FLOWERY BRANCH - Jonathan Babineaux seized the opportunity to remind an impatient Peria Jerry about post-practice protocol in the NFL.
FLOWERY BRANCH - Roddy White practiced for the first time Monday as one of the highest-paid receivers in the NFL.
FLOWERY BRANCH - After holding out for the first week of training camp, receiver Roddy White agreed to a contract extension with the Atlanta Falcons on Saturday night.
SNELLVILLE, Ga. - Quarterback Matt Ryan doesn't know how the Atlanta Falcons will be without Roddy White, so he used a scrimmage Friday night to work with other receivers.
The Atlanta Falcons signed free agent wide receivers Marty Booker and Robert Ferguson, released wide receiver Bradon Godfrey and placed wide receiver Harry Douglas on injured reserve.
FLOWERY BRANCH - Rookie safety William Moore, the Atlanta Falcons' second-round pick from Missouri, is expected to miss two to four weeks following arthroscopic surgery on his left knee.
FLOWERY BRANCH - The Atlanta Falcons' depth chart at wide receiver doesn't look much like coach Mike Smith planned.
The sound you might have heard coming from South Hall on Thursday was the first shoe dropping. Now, Falcons fans sit and wait for the other one.
FLOWERY BRANCH - An injury to Harry Douglas and the continued contract holdout by Roddy White have combined to make wide receiver a position of rising concern for the Atlanta Falcons.
FLOWERY BRANCH - Pro Bowl receiver Roddy White is working out with former UAB teammates in Birmingham, Ala., while holding out for a new contract with the Atlanta Falcons.
FLOWERY BRANCH - Mike Peterson finally made peace with himself after last year's meltdown with Jacksonville coach Jack Del Rio.
FLOWERY BRANCH - Long after the rest of the team hit the showers, the members of the Atlanta Falcons secondary remained on the practice field working on technique.
FLOWERY BRANCH - As much as Pro Bowl running back Michael Turner appreciates his five offensive linemen in Atlanta, Harvey Dahl and Tyson Clabo have a special place in his heart.
FLOWERY BRANCH - Tony Gonzalez has a hard time believing quarterback Matt Ryan is only entering his second NFL season.
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Scientific research and entrepreneurship in clean energy is alive and well at the ARPA-E Summit.
At the ARPA-E Energy Innovation Summit, startups and research organizations show off the latest inventions in the multiple paths to cleaner energy.
The DOE's ARPA-E is funding energy research and development but the agency is seeking to engage large industrial companies to bridge the gap from lab to market.
The ARPA-E Summit showcases research in several clean-energy technology areas which the Department of Energy hopes to yield results from in a few years.
The ARPA-E Summit opens today to tout potential game-changing clean energy technologies and convince Congress to further fund the program. Entrepreneurs say the program helps long-term projects.
Looking to highlight the fruits of investing in energy research and development, the Department of Energy says six ARPA-E grants attracted four times more money from the private sector.
Energy Secretary Steven Chu visits ARPA-E grant winner 1366 Technologies, a tiny solar start-up shooting to make solar as cheap as coal.
Your guide to the ARPA-E Energy Innovation Summit this week, where startups and research institutions are showing off the latest inventions in cleaner energy.
ARPA-E, the Department of Energy agency created to research clean-energy breakthroughs, puts an additional $106 million into 37 commercial and academic ventures.
The ARPA-E program is funding promising cutting-edge research, but there are financial and policy barriers for getting those technologies to a meaningful scale.


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Compatibility: Requires iOS 8.0 or later. Compatible with iPad.


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Cellular Signalling Editorial Board
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Hannah's Bend Park
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Year Established:
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Scenic holes with dual tees overlooking the Cannon River. Very good for beginners, sometimes teasing experts if it's windy. Some trees, hills.
Course Details
Course Type: Permanent
# Holes: 9
Target Type: Mach III
Tee Type: Grass
Elevation: Flat
Foliage: Scattered
Course Length: 2,405ft
Alternate Length: 0ft
Hole Length: Under 300ft: 7 | 300 - 400ft: 2 | Over 400ft: 0
Course Designer: Chuck Kennedy and Chuck Cadwel
About 6 blocks north of Main St. on 9th St. via 6th St.; by swimming pool.
First Tee: (
) -92.911989,44.513546
Email: Chuck Kennedy
Phone: 651-455-1217
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Tuesday, August 11, 2015
3 comments
Well my clothesline ended up laying on the ground after a little wind yesterday. My neighbor got it back in the ground but it needs some more cement around the base. Hope we can get that done after the rain stops in a couple days. I'm so anx... Read more
Sunday, August 09, 2015
3 comments
After my surprise birthday party on Tuesday, my DD and her boyfriend had one more trick up their sleeve today.
At the beginning of summer, she used my c... Read more
Saturday, August 08, 2015
2 comments
Oh how I wish I would blog more often! But anyway, I am lovin the summer even though it is cooler (but more comfortable ) than usual and it is so very short! The last few days have felt like Fall. I love Fall, its my favorite season, and I l... Read more
Monday, April 20, 2015
6 comments
Saturday, March 21, 2015
6 comments
I thought I had breezed through winter without the winter blues, even though I did get 4 colds and one maybe was the flu, but the last 5 days sre making me wonder. I have gotten up in the morning and looked out the window to see snow, a good am... Read more
Monday, February 09, 2015
4 comments
The winter blues usually gets me good in January and February. This year hasn't been as bad as usual for some reason but I still have my moments.
Today was one of those days. I saw more snow coming down and was wishing it was warmer and t... Read more
Monday, February 02, 2015
4 comments
CORRECTION........Hubby said the animal I saw yesterday was a coyote. He said they are in the woods accross the street. My neighbor said they have come down into the mobile home park before too.
So whatever it was, it still scared me. I w... Read more
Sunday, February 01, 2015
5 comments
Well in an effort to start pulling myself out of a rut, no exercise and endless snacking, I decided to go for an early morning walk. I bundled up as it was 20 degrees and my coat is heavy. I kinda looked like that younger kid on Christmas Stor... Read more
Friday, December 26, 2014
7 comments
Awhile back when Spark added more point levels I decided that I had worked too hard to have to try to reach higher levels. I decided to try a different weight loss site.
I shouldn't have! I didn't lose anything, in fact I added some we... Read more
Monday, August 25, 2014
6 comments
I have always loved going to our State Fair, so why did I not like it this year?
Maybe because we got a late start from having to pick up our rental car this morning. (We had an accident with our van on the 13th and it's in the shop now)... Read more
Wednesday, August 06, 2014
7 comments
Thursday, July 03, 2014
6 comments
My kitty Maxie is doing well and letting me squirt the wash solutipn and put the antibiotic cream on his cuts. He is acting almost his normal silly self again. Thank you all f... Read more
Friday, June 27, 2014
7 comments
I know it's been a long time since I blogged and I apologise for that. I guess I have been adjusting to our new home and then being outdoors working in my herb garden and
making new friends when I go on my many daily walks.
And I am a... Read more
Wednesday, February 19, 2014
10 comments
Thank you for all the encouraging comments. I know now that I`m not the only one struggling with the winter this year.
Today was a "warmer" day, in the 40s, so I got out there and walked, breathed in the fresh air and shed some stress. It ... Read more
Tuesday, February 18, 2014
6 comments
So I started January with a great outlook. February is half over and where did the outlook go bad?
I have gone even further away from my goal and I`m not even counting anymore. I still log my food but it usually goes over what I should h... Read more


text:
Distribusion Wants To Make Selling Intercity Bus Tickets As Easy As Selling Flights
Similar to SilverRail, the startup that is building a platform to consolidate passenger rail ticket booking and search across Europe and beyond, newly-launched Distribusion wants to make it as easy for travel companies to sell 'intercity' bus tickets as it is to sell flights. Read More
Busbud Bags $9M For Its Coach Travel Booking Platform
Global coach travel-booking platform Busbud has closed a $9 million Series A funding round co-led by Omers Ventures and Revolution Ventures. iNovia Capital and Real Ventures also participated — having co-led Busbud's prior round of $1.2 million back in May 2013. Read More
Busbud, The Kayak For Intercity Bus Travel, Raises $1M Round Led By iNovia Capital And Real Ventures
Canadian startup Busbud, which bills itself as the "Kayak for intercity bus travel," today announced that it has raised a first $1 million funding round led by iNovia Capital and Real Ventures. Peter Kern, an Expedia board member and managing partner of private equity firm InterMedia Partners, as well as Luxury Retreats CEO Joe Poulin also participated in this round. iNovia… Read More


text:
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April 8, 2008
LSU's Randolph declares for NBA Draft
BATON ROUGE, La. LSU freshman forward Anthony Randolph intends to enter the NBA draft, although he has not hired an agent and is leaving open the possibility of returning to the Tigers for his sophomore year.
"There cannot be a better time for me to enter the NBA draft," Randolph said Tuesday. "I am well aware of the challenges young men face when entering the professional ranks of any career. However, I believe I am ready to face these challenges with a positive attitude, and I have faith and determination that I will succeed."
The 6-foot-10 Randolph averaged 15.6 points, 8.5 rebounds and 2.3 blocks this season. LSU was 13-18 this season, and coach John Brady was fired near the end of the regular season.
Randolph said the uncertain coaching situation had nothing to do with his decision.
"I just know that I have the opportunity of being selected in the first round," he said. "I was counseled by my family and the coaches and we all came to the conclusion that right now would be the best time for me to pursue my professional career."


text:
The Princess of Mononoke by fukamatsuWatch
©2011-2016 fukamatsu
I hope they can meet again in the future.
I hope that they can meet again, too
So beautiful!
This is so beautiful! Love your painting style and colours....and the blurred foreground works so well...absolutely lovely!
This is amazing, great job!
Don't worry, they'll meet again! Ashitaka said he'd visit San. I should know, after hearing him promise that, I've kept imagining possible post-movie scenarios. X3
this is lovely!! what app did you use?
one of the best studio ghibli movies i love their characters
great pic
great pic
such a good movie!
wow, one of my all time favorite of Ghibli studio's animation, nice job!
Kyeta: My brother and I watched this movie when we were kids. Same with the movie "Spirited Away".
Wow! I really like the way they look. So free and alive. Love your colors too.
I hope they can meet in the future too! >.<
love the colours, the looseness and the action in your drawing! ^^/
love the colours, the looseness and the action in your drawing! ^^/
I LOVE THIS MOVIE! It's my favorite Miyazaki film
OoO wow, so pretty XD
This one is in my favourite too !!
Amazing !
Amazing !
Wow I love this! ^-^
I hope so too!
The one Miyazaki movie I wished had a sequel
The one Miyazaki movie I wished had a sequel
beauuutiful! I love the colours and the little ladybug too! *-*
SO Beautiful *_*
gorgeous! Great combination of colors and motion! lovee it
thats looks very beautiful!
ikr!! and have awsome baby's that kick ass and help the earth!
In response to your description, me too. ; A ;
Lovely focus on them, by the way. <3
Lovely focus on them, by the way. <3
Really nice job on this! the colors go together wonderfully; it's nice to see some great work on one of my favorite movies ever! <3
nice i love it
This is gorgeous! Lovely detailing. Very beautiful nice work~!! This is one of my most favorite films. You did it wonderful justice!
The colors are so pretty!
I totally forgot about the movie till i saw this picture -digs through movie rack to find it- Awesome!
I love this picture!!!! and the move are soo cute
wow I love the colors you used here! *w*
Beautiful work, but it's just Princess Mononoke, not The Princess of Mononoke.
I totally LOVE the colors! *__*
One of my favorite movies of all time!
I adored soft colors and all, is great work >u<!
Really, really beautiful. Nice choice of colours. ; u ; <3
Embodies the strong, beautiful spirit of the adventure.
I do, too! Man, this sure is beautiful... I love your thin lines, and all those fluttering leaves!
very nice
love the colours!
love the colours!


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Wednesday, September 9, 2009
Best Of The West!
Where we love to fly
Jessica Ambats, Editor
Airport: Page Municipal Airport (PGA)
Activities: Jet-skiing (through rock canyons), houseboat cruising, swimming, mountain biking and secluded camping. (Lake Powell has nearly 2,000 miles of shoreline!)
Best Season: Year-round
Flying Tips: Check density altitude during the summer. If you're arriving from the south/southwest, the most scenic route is via the Grand Canyon VFR corridors. Continue northeast over Marble Canyon into Page, Ariz.
What to Bring: Bathing suit, sunscreen, camera
URL: www.lakepowell.com
Mammoth Lakes, California
Jeff Schroeder, Advertising Manager
Airport: Mammoth Yosemite Airport (MMH)
Activities: In the summer, fishing, hiking, mountain biking and golfing. In the winter, skiing, skiboarding and…skiing!
Best Season: Year-round
Flying Tips: Watch out for strong winds coming over the Sierra Nevada mountain range throughout the Owens Valley. Also, there's no runway snow removal at night.
What to Bring: All of your outdoor sports equipment, but if you forget it (or can't fit it), you can buy or rent anything imaginable there.
URL: www.mammothmountain.com
Our Favorite European Destination!Lago di Como, Italy
Jim Lawrence, LSA Editor
Airport: Aero Club Como (water landings only!) and Milan Malpensa Airport (MXP)
Activities: Get your seaplane rating at Lake Como, Italy's only seaport! Windsurfing, sailing, boating, paragliding, celebrity sighting and shopping.
Best Season: March through October
Flying Tips: Study up on local aviation regulations.
What to Bring: Recreational gear, web-enabled cell phone, guidebooks
URL: www.aeroclubcomo.com
Page 3 of 3
Labels: Decision Making, Features, Journeys, People and Places, Travel


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Original Research ARTICLE
The genome sequence and effector complement of the flax rust pathogen Melampsora lini
- 1CSIRO Plant Industry, Canberra, ACT, Australia
- 2The Sainsbury Laboratory, Norwich Research Park, Norwich, UK
- 3Research School of Biological Sciences, College of Medicine, Biology and Environment, Australian National University, Canberra, ACT, Australia
Rust fungi cause serious yield reductions on crops, including wheat, barley, soybean, coffee, and represent real threats to global food security. Of these fungi, the flax rust pathogen Melampsora lini has been developed most extensively over the past 80 years as a model to understand the molecular mechanisms that underpin pathogenesis. During infection, M. lini secretes virulence effectors to promote disease. The number of these effectors, their function and their degree of conservation across rust fungal species is unknown. To assess this, we sequenced and assembled de novo the genome of M. lini isolate CH5 into 21,130 scaffolds spanning 189 Mbp (scaffold N50 of 31 kbp). Global analysis of the DNA sequence revealed that repetitive elements, primarily retrotransposons, make up at least 45% of the genome. Using ab initio predictions, transcriptome data and homology searches, we identified 16,271 putative protein-coding genes. An analysis pipeline was then implemented to predict the effector complement of M. lini and compare it to that of the poplar rust, wheat stem rust and wheat stripe rust pathogens to identify conserved and species-specific effector candidates. Previous knowledge of four cloned M. lini avirulence effector proteins and two basidiomycete effectors was used to optimize parameters of the effector prediction pipeline. Markov clustering based on sequence similarity was performed to group effector candidates from all four rust pathogens. Clusters containing at least one member from M. lini were further analyzed and prioritized based on features including expression in isolated haustoria and infected leaf tissue and conservation across rust species. Herein, we describe 200 of 940 clusters that ranked highest on our priority list, representing 725 flax rust candidate effectors. Our findings on this important model rust species provide insight into how effectors of rust fungi are conserved across species and how they may act to promote infection on their hosts.
Keywords: rust, flax, Melampsora, effector, virulence, avirulence
Citation: Nemri A, Saunders DGO, Anderson C, Upadhyaya NM, Win J, Lawrence GJ, Jones DA, Kamoun S, Ellis JG and Dodds PN (2014) The genome sequence and effector complement of the flax rust pathogen Melampsora lini. Front. Plant Sci. 5:98. doi: 10.3389/fpls.2014.00098
Received: 13 January 2014; Accepted: 28 February 2014;
Published online: 24 March 2014.
Edited by:Sébastien Duplessis, INRA, France
Reviewed by:Pietro Daniele Spanu, Imperial College London, UK
David L. Joly, Université de Moncton, Canada
Copyright © 2014 Nemri, Saunders, Anderson, Upadhyaya, Win, Lawrence, Jones, Kamoun, Ellis and Dodds. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Peter N. Dodds, CSIRO Plant Industry, GPO Box 1600, Clunies Ross Street, Canberra, ACT 2601, Australia e-mail: email@example.com


text:
How to make Harry Potter by VitaazWatch
©2011-2016 Vitaaz
Hi. So cute - really clear instructions. On my must try list. Thank you for sharing
This is adorable! I'm gonna have a go at making him myself.
so cute thanksfor the tutorial ^_^
Nice tutorial.
oo I have to try that!
Pretty cool!
it's so cute
this is adorable and looks so simple o_O
Really great! Thanks for the tutorial!
so cute!!!
Wow, it's great! Easy and good looking as well ^w^ And i really adore your idea with the glasses I tried to do them out of wire, but they were rough :/
Thanks for this tut, gonna use it in the future!
Thanks for this tut, gonna use it in the future!
ahahaha awesome!!!
So cute!^^
it's veryyy cuteee! ^^ aaww
totally cute will try one day
This is awesome
Ohmygosh SO CUTE!!!!
I love this!I'ts sooooo cute!
I love this!I'ts sooooo cute!
that's so cute and looks very easy! xD
That's awesome!
so cute
This is really cool!!
Cute! I wanna try to make one now.
cute work! i wanna see more detail photo for how you did his hair... XD
Awww he's so cute!


text:
- Find a rental
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- Burlingame rentals
- How to book
- Help Center
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San Francisco is nearby, not the fog!
From $165 / night
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Description from the owner
Description from the owner
Bedrooms: 1/
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Sleeps: 2/
Type: Apt. / Condo
Located in Burlingame..in the sunny mid Peninsula.
An old style neighborhood, where you can stroll in the sunshine to the local stores & restaurants, we rarely have foggy days.
Hop on the local trolley bus, and explore the Avenues upscale venues, Apple, Pottery Barn. Gap, wonderful local boutiques, and so many more!
or walk to the Bay Trail, with views to San Francisco and the East Bay, watch the amazing sunsets whilst enjoying a night cap at a local watering hole!
Easy Freeway access or train or bus to San Francisco or San Jose.
Minutes to SFO!
This one bedroom suite has original 20s hardwood floors, a designer bathroom, and separate living ...Read more
About the owner
About the owner
Maggie & Stan
Maggie & Stan
- Response rate : 100%
- Response time: A few hours
- Calendar updated: 6 days ago
Overall rating:
Additional Location Information
Burlingame is friendly neighborhood, with lots of small shops and wonderful restaurants within level walking distance. A short ride (or healthy walk) is Burlingame Avenue where all the upscale shops can be found, Apple, Pottery barn, Gap, Anthropolgie etc and lots more restaurants!
A quick hop on the freeway will whisk you in to San Francisco (public transport too!)
We are minutes from SFO but not the flight path.
Parking is fine on the street.
More About This Location
City vacations, Lake nearby, Mountain nearby, Warm winter getaways
Getting There
Nearest train station: Millbrae, 1.5 miles
Nearest airport: SFO, 1 miles
Car is not necessary
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text:
Assume that you want to purchase a straddle on a given stock because of some anticipated news that you expect will move its price significantly. You create a neutral straddle 6 days before the expected news by buying puts and going long the stock. But before the news comes out, the stock rises slightly, and this changes the deltas of the puts and therefore the delta of the overall position. The table below gives an illustration.
Continuing with our example, you are considering buying calls on DOG and have a target price in the 107 –108 area. This is approximately where the returns on the 90 and 100 calls are equal. Should you be indifferent between these two options? Before answering this last question, look at the 3 options' break-even points, as given below.
The break-evens (equal to the strike price plus the premium) give you additional information. If you expect DOG to rally to the 107 – 108 area you could be indifferent as to which option, the 90 or the 100, you purchase. But look at the break-even points above. The 90 calls break-even at a point about $3.50 lower than the 100 calls. This would tend to tip the scales in favor of the 90 calls. If DOG rallies to 108, your return will be as forecasted. But if the rally fizzles and DOG only climbs to 102 or 103, the 90 calls will still leave you with something to show for your effort.
Note that our analysis does not include the price of the options as a decision-making variable. Yes, the 90s are more expensive than the 100 calls, but if they generate a higher return on capital at risk, then your investment objective will be met.
Calculating cross-over and break-even points when trying to decide which call option to purchase can only help you make a more informed decision.
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text:
Organisation Development and Transformation in South Africa
LexisNexis Butterworths, 2004 - Organizational change - 455 pages
From inside the book
37 pages matching Competence Process in this book
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1 Definitions of organisation development
1 Major topics associated with organisation development
2 Fundamental differences between OD and OT
50 other sections not shown
Common terms and phrases
achieve action research activities alignment analysis approach assessment balanced scorecard behaviour business ethics challenge change process chapter client collaborative commitment communication Competence Process competitive components Compupro consensus decision-making consultant corporate create creativity decision-making decisions defined diversity management effective emotional intelligence employees employment equity ensure environment environmental scan evaluation example execution external facilitate feedback focus function goals human resource identified impact implementation important improve individual innovation integrated internal interventions involved knowledge management labour turnover leaders leadership learning organisation measures ment moral needs objectives organisation development organisation's organisational culture outcomes participants performance appraisal performance management performance management system phase potential practices practitioners problem solving productivity programmes Randburg relationship responsibility role skills development Social Processes South African specific stakeholders strategy structure success talent management tion transformation Ubuntu values vision well-being workforce workplace


text: This is Simone, who runs a great menswear store in Florence called Tie Your Tie (okay, so the name needs a little help). What I love about him is that he's so classically Italian in the cut and proportions of his style, but he never looks like he's wearing a uniform. While most of his countrymen favor super-creased, stiff fabrics, Simone seems to wear only the softest, most textural suits and jackets. Imagine crossing crepey Armani fabrics of the mid-80s with the modern Italian tailored silhouette and you create the perfect storm of sophistication and ease. It's a look worth trying back in the States.


text:
Well, it's been a year now since I've purchased a new phone, and well, I've been getting gear anxiety. A few co-workers in the office have the new iPaq h6315 and the Samsung SPH-i700 device. However, they are both to large for my tastes. AT&T has been selling the Audiovox 4100 for a while, and I've been holding out for the Motorola MPX which was delayed yet again. When the MPX does come out, if it's not outrageously expensive I'll buy it and sell my Audiovox.
So, I went down to AT&T and got a Audiovox 4100. I have to say, this device rocks. Sure it doesn't have Wifi or bluetooth, but with my all you can eat GPRS data plan it's not such a big deal. I've owned 2 Pocket PC devices in the past and I'm glad to be back in the Pocket PC camp, and out of the Smartphone camp. There are a number of reasons that I think the PPC device is a better fit for me. Among them are:
Personally, I loved my Smartphone, but realistically it was a read only device. Also, the Tasks feature was fairly useless. The Audiovox device is small, and as a result fits fine in my pocket (I will not purchase any PDA/Smartphone device that will not fit in my jeans).
Finally, having just discovered Microsoft Voice Command, I can't imagine not living with it. Voice Command is like your own personal assistant. This is hands on one of the best Microsoft products out there. I installed it this morning, and activated voice and it has recognized 100% of my commands such as:
This is truly an innovative, compelling product that really showcases the power of the platform. Check out the flash demo.


text:
Jenkins Sorcerer - Howl's Moving Castle by FeeracieWatch
©2012-2016 Feeracie
I love this movie and I think he is hot
Love this movie too ! And yeah for sure Howl is so elegant ~:3
thanks so are you doing anymore of him
Yeah i will!
ok can i give you another idea if that's ok
Yeah for sure, wil do a lots of his outfits this year o/!
great job awesome cosplay!
Amazing picture
So cool!! It's just like his house!!
Omg that is so awesome! How on earth did you find that building? It's truly epic!
Can I please hug you? c:
hauru-sama!!!!! you look good...
I like it so much ) Where`s you got this door? It`s perfect *-*
Thank you ! D We found this door in Paris :3
Oh, I wanna go there just for look at it *-* The same as in anime was!
It's a little place near from Montmartre(if you have the opportunity to go to Paris :3) But you have such beautiful location in Russia too! "_"
Oh, I went Paris some years ago! Such beatiful city :-З Do you live in Paris?
Oh, thanks ^^ Yes, we have some excellent places for cosplay photoshooting in Russia *-*
Oh, thanks ^^ Yes, we have some excellent places for cosplay photoshooting in Russia *-*
This photo looks soo cool !!


text:
If you have a project to help communities or the environment within the Borough of Fareham you might be able to get funding for it. We set up the community fund to help you make this area an even better place to live. It aims to help useful projects get off the ground.
Any resident or organisation based in the Borough can apply for money from the fund. Your project must support or benefit our local communities or the environment.
Before applying to the fund, you must meet certain criteria:
The fund is not for:
Unfortunately we are unable to accept applications from organisations that have received funding from Fareham Borough Council within the 12 months before your application.
You can apply for any amount but if you are seeking more than £5,000, you will be expected to contribute at least the same amount of money to the project from other sources.
To find out how you can apply for funding please click here.


text:
Are you Yun-Chao Chen?Claim your profile
Publications (6)6.23 Total impact
- [Show abstract] [Hide abstract]
ABSTRACT: It is a globally challenging problem to differentially diagnose biliary atresia (BA) from other disease processes causing infantile cholestatic jaundice. The high-frequency ultrasonography (HUS) yields much improved spatial resolution and therefore, might show better image in BA diagnostic examination. The present study was to evaluate the HUS on the diagnosis of BA in infants with jaundice. Fifty-one infants with neonatal jaundice were scanned with ultrasonography. Images included gallbladder, bile duct, right hepatic artery (RHA), portal vein (PV) and triangular cord (TC) sign, magnetic resonance imaging and additionally, laboratory tests and histopathology reports were assessed. Twenty-three BA and 28 non-BA cases were confirmed. The sensitivity, specificity, and accuracy of HUS were 91.3%, 92.9%, and 92.2%, respectively. All of these indices were significantly higher than those of conventional ultrasonography (P<0.01) and MR cholangiopancreatography (P<0.05). The HUS features, included a positive TC sign, an increased RHA diameter and RHA-diameter to portal-vein-diameter ratio (RHA/PV) and abnormal gallbladder, were important in the diagnosis of BA. HUS provided better imaging of BA and should be considered as a primary modality in the differential diagnosis of infantile jaundice.
Conference Paper: Ultrasound Irradiation Accompanied with PEI Mediated Gene Transfection: In Vitro Experimental Study[Show abstract] [Hide abstract]
ABSTRACT: PURPOSE To investigate whether ultrasound and PEI can enhance plasmid DNA transfection in HepG2 cell synergistically or not. METHOD AND MATERIALS Two kinds of PEI, which one used specifically in gene delivery(sPEI)and the other used just as general industrial materials(iPEI), were used. The toxicity of PEI was determined by trypan blue dye exclusion. The PEIs were incubated with the plasmid DNA to prepare cationic compounds (PEI/DNA), which were added into HepG2 cell with or without ultrasound irradiation. After 24 hours, the transfection efficiency was assessed by fluorescence microscope and FACS. Ultrasound parameters: 1 MHz, 1W/cm2, 20s, 20% duty cycle. RESULTS Ultrasound could induce gene delivery in HepG2 cell. PEI displayed high toxic to cell and the concentration of 0.001% was used in gene delivery, which induced about 70% cell survival rate. The two PEI compounds significantly enhanced the plasmid DNA gene delivery in HepG2 cell(P<0.01) and the transfection rates of the iPEI and sPEI were 15.42±4.71 and 12.27±3.58 respectively. Even though the difference of the two PEI was not significant(P>0.05), their transfection rates were higher than that of the ultrasound group(P<0.01). On the other hand, PEI with ultrasonic irradiation group showed a lower transfection rate compared with the PEI groups(P<0.01) CONCLUSION PEI and ultrasound irradiation all can promote plasmid DNA transfection in vitro respectively but they do not show synergistically. CLINICAL RELEVANCE/APPLICATION PEI and ultrasound irradiation all can promote plasmid DNA transfection in vitro respectively but they do not show synergistically.
Article: Enhanced Gene Transduction into Skeletal Muscle of Mice In Vivo with Pluronic Block Copolymers and Ultrasound Exposure[Show abstract] [Hide abstract]
ABSTRACT: The pluronic block copolymers are able to enhance the ultrasound-induced gene delivery in vitro. In the present study, the effects of pluronics on the efficiency of gene transfer into skeletal muscle in vivo under sonoporation were investigated. Plasmid DNA encoding green fluorescent protein (GFP) in combination with three different pluronics, F127, L61, and P85, was injected into the tibialis anterior (TA) muscle of mice with and without adjunct ultrasound (1 MHz, 3 W/cm(2) 1 min, 20% duty cycle). Mice were killed 1 week after injection. The TA muscles were removed and snap frozen immediately in isopentane cooled by liquid nitrogen and sections of 7 μm thick were cut. Transfection efficiency was assessed by counting the number of GFP-positive fibers under fluorescence microscopy, and tissue damage by hematoxylin and eosin staining. The results suggested that all three pluronics significantly enhanced transgene expression in skeletal muscle (P < 0.01), especially the P85 showed significantly higher efficiency than the other two pluronics (P < 0.05). Ultrasound synergistically enhanced the gene delivery efficiency with P85 (P < 0.01), but was unable to do so with F127 and L61 groups. In short, P85 displays significantly synergistic effect with ultrasound for enhancing plasmid DNA transduction in skeletal muscle of mice in vivo.
Article: Pluronic block copolymers: Novel functions in ultrasound-mediated gene transfer and against cell damage[Show abstract] [Hide abstract]
ABSTRACT: Pluronics have been investigated as vectors for drug and gene delivery in vitro and in vivo and were demonstrated to have high efficiency for gene transfer in vivo. However, they alone do not enhance gene transfer in vitro. We examined three pluronics, F127, L61 and P85, for their effects on ultrasound (US)-mediated gene transfer in three cell lines, 3T3-MDEI, C2C12 and CHO. The polymers showed differential effects on cell viability and transfection efficiency in a dose-dependent manner. All the polymers were unable to facilitate gene transfer when used alone, but enhanced US-mediated gene transfer significantly at concentrations around the critical micelle concentration in the three cell lines. F127 showed no significant toxicity at any concentration and protected the cells against US-mediated damage at a high concentration. L61 decreased cell viability significantly in a dose-dependent manner, whereas P85 showed mild toxicity when its concentration was at or above 0.05%.
Conference Paper: Mechanism of Sonoporation with Ultrasound: How Long Does the Permeabilisation Last?[Show abstract] [Hide abstract]
ABSTRACT: PURPOSE It has been known for over a decade that ultrasound can aid in the delivery of molecules and genes into cells through a process of cell membrane permeabilisation (sonoporation). The underlying mechanisms however remain unclear. Evidence from electron microscopic images has suggested a role for relatively long lasting pores: (Tachibana et al. 1999). We have studied the duration of the permeabilisation using a fluorescent macromolecule tag. METHOD AND MATERIALS Chinese Hamster Ovary cells (CHO) were exposed by ultrasound (2W/cm2 for 10 seconds, 20% duty cycle, 1MHz). We added equivalent quantities of the fluorescent macromolecule FITC Dextran (66k Da) (A) prior to insonification (B) within the first second of insonification (C) at medium time points (2s, 20s, 60s, 10min, 20mins) (D) at 1hour and later after insonification. The level of uptake was assessed using flow cytometry. RESULTS High uptake (85%) was seen when the FITC Dextran was present prior to insonification. Much lower values were seen when it was added after insonification from 2s to 20 mins (all values under 20%). This occurred even when added within the first second that ultrasound was applied (uptake of 10%). A monotonic progression in uptake with the delay from exposure was seen, with declining uptake with longer delays. Cell viability remained moderate at all exposure levels. Interestingly however, after a recovery period of 1 hour or more after insonification, the internalization of the macromolecules was comparable to the positive control. CONCLUSIONS These data suggest that the sonoporation effect is a non-linear process. The difference between conditions (A) and (B) suggests a dramatic alteration of permeabilisation may occur within the first second. The temporal changes over the medium time frame under conditions (C) may relate to slow resealing. The reasons why higher uptake was seen at later time points over an hour (conditions D) is unclear, and is the subject of further investigations. These observations may be useful in planning the use of sonoporation both with and without microbubbles for gene therapy and drug delivery in guiding the timing of ultrasound exposure.
Article: The value of color Doppler ultrasonography in monitoring normal orthotopic liver transplantation and postoperative complications[Show abstract] [Hide abstract]
ABSTRACT: To assess the value of color Doppler ultrasonography in monitoring normal orthotopic liver transplantation and postoperative complications. Forty-one patients after orthotopic liver transplantation were examined by using color Doppler flow imaging to observe the hepatic blood flow and change of ultrasonography of the hepatic parenchyma and bile duct. The measured indexes included maximum blood flow velocity, time-average blood flow velocity (TAV), resistance index (RI) and diameter of the bile duct. Among 41 patients, 17 (41.5%) suffered from liver transplant rejection. Of the 17 patients, 13 (76.4%) showed decrease of TAV of the portal vein, 15 (88.25%) low-amplitude single-phase serrated wave or negative biphasic wave of the hepatic vein, 9 (52.9%) increased hepatic arterial RI, and 5 (29.4%) slightly dilated bile duct. Sonography showed disappearance of the hepatic artery blood flow around the portal vein in 5 (12.2%) of the 41 patients with hepatic artery thrombosis in the postoperative period. Slight dilatation of the intrahepatic bile duct was found in 3 (7.3%) of the 41 patients in the early postoperational period and it normalized within 2 weeks. Ultrasonography of 20 patients (48.8%) revealed a visible dilatation of the intrahepatic bile duct, which was worsening gradually. The causes of bile duct dilatation included biliary stricture in 2 patients (10%), stone in 15 patients (75%) and others in 3 patients (15%). Color Doppler ultrasonography is valuable for monitoring normal liver transplantation and postoperative complications.
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Tongji HospitalWu-han-shih, Hubei, China
Imperial College London
Londinium, England, United Kingdom
- Department of Imaging Sciences


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The reason the X Games crowd prefers loose-fitting outerwear isn't just that it looks cool. It's also more comfortable, especially in the air. The waterproof-breathable, uninsulated Bomb Pop features a baggy fit that allows for freedom of movement whether you're launching off a cliff or jibbing your way through the park. Even better, it's packed with features—tall collar, giant hood, powder skirt, wrist gaiters—that make it just as user-friendly for rallying groomers as it is for hunting down fresh snow. But at two and a half pounds, it's probably best worn in-bounds. 2.5lbs
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July 10, 2007
Five on the rise
Several prospects around the nation are finding that their stock is on the rise as a result of their performances at recent camps and combines. We take a look at five players who are really coming on in the class of 2008. Watch for the recruiting battles to heat up and a potential rise in the rankings for these prospects.
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MEC is pleased to announced that Shenan Reed, President Digital, MEC North America has been selected by Advertising Women of New York (AWNY) for the 2016 'Changing the Game' Awards, honoring women who are true catalysts of innovation. Shenan has been named within the 'Quantum Leap' category, which recognizes woman who are changing the way organizations are aligned to pro-actively meet new challenges. Shenan's vision to move beyond 'digital' by constructing strategies for a digitally-enabled world has successfully guided clients through the seismic shift that digital and data is having on their business.
Shenan has also received honors from iMedia, named Agency Executive of the Year in 2015 as well as a 2015 AWNY Game Changer.
Commenting on the award, Shenan stated "To quote one of my favorite authors, 'I can no other answer make but thanks, and thanks; and ever thanks.' - William Shakespeare. I am humbled to serve as the recipient of this award but I am merely the one who is representing the work, passion and dedication of the many. This is for my team, partners and co-workers who help me thrive."
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Dale Keenan has chance to make a bid for the left-back slot after Aaron Taylor-Sinclair's move south
Christopher Jack, Group Senior Sports Writer / / Sport
CONRAD BALATONI today tipped Partick Thistle youth Dale Keenan to make a bid for the club's left-back berth this season.
The Firhill defender was handed a new deal by boss Alan Archibald earlier this summer and has spent the last few weeks training with the Jags first team ahead of the new Premiership campaign.
Archibald remains on the lookout for defensive reinforcements as he bids to bolster his squad for Thistle's second shot at Scotland's top flight next term.
He ran the rule over another contract hopeful as his side lost 1-0 to Rangers in a behind-closed-doors clash at Firhill on Tuesday as he looks to replace Aaron Taylor-Sinclair following his move to Wigan.
Keenan has been given his chance to catch the eye in friendly action so far after impressing for Scott McKenzie's under-20s last time out.
And Balatoni has urged his fellow defender to make the most of the opportunity in front of him as Archibald continues his search for fresh faces in the coming weeks.
He said: "I don't know what is happening in terms of new additions. I am sure the manager will get everything sorted out in the next few weeks and the squad will be firing on all cylinders.
"A left-back is obviously the main one because of Sinky leaving, but young Dale Keenan is more than capable of coming in if we don't get another player in.
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"I am sure the manager has got targets and it is down to him to get it finalised, we just carry on with the football.
"Dale is a good player and I said to him a few weeks ago that if he does well in pre-season then he will give the manager something to think about.
"He has done well every time he has come on and I have been impressed with him.
"It is down to him to keep his head down and work hard. If another left-back does come in, if he gets a chance he will need to keep it and cement that place as his own."
Finding someone to fill the Firhill No.3 jersey is Archibald's top priority in the coming weeks as the countdown continues to the new Premiership campaign but he also remains in the market for a centre-back and striker this summer.
Balatoni enjoyed a solid debut campaign in the top flight last term and is likely to be one of the first names on the team-sheet when Thistle host Ross County in their league opener next month.
The Jags head south of the border to face Fleetwood Town this weekend before the visit of Sunderland next midweek rounds off their summer schedule and Balatoni is confident he will be in prime shape for the big kick-off.
He said: "I played for 45 minutes against Rangers. I am feeling good. It was another good half under my belt and I feel that I could play 90 minutes.
"I played on Sunday and I would like to get 90 minutes in the next couple of games.
"The manager has worked us hard and I am feeling fit. I would like to play the full games against Fleetwood and Sunderland but I will see what the manager's plans are.
"The season is not far away now and I want to play as much as I can.
"You don't want to tire yourself out before the first league game, but I am feeling good and hopefully the manager will see that I can play the two games."
Thistle have a couple of extra days to prepare for their first Premiership outing this term after their clash with champions Celtic on the opening weekend of the campaign was postponed. And Balatoni is determined Archibald's side will get off to a flying start when they do finally get underway on August 13.
He said: "It is a bit disappointing that the first game has been postponed, but that is life and we have to deal with it.
"It gives us an opportunity to get the three points in our first game at home to Ross County.
"Everyone will be fired up for that and looking to get off to a good start.
"Hopefully we come through the next couple of weeks and games unscathed and we can focus on getting the three points in the first game."
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Work without a vision is slavery, Vision without work is a pipe dream, But vision with work is the hope of the world.


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January 28, 2014
Looking good
With two signed Division I pitchers, a Division I catcher plus a junior shortstop who was awarded a spot on the national gold glove team a year ago along with a 0.00 ERA in 21 innings pitched, there is little doubt that Blue Valley West will get the nod as the No. 1 team in Kansas this season to begin the season.
West returns nine starters from last year's 21-4 Class 6A champions including four who spent at least 15 innings on the mound.
Even as tough as Manhattan, Wichita Northwest, Maize and several others look this year, they will get the nod.
Check out the inset and see what Prepl Baseball Report of Kansas has to say about them.
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Preston voters will soon face the most important decision about the former Norwich Hospital property since voting in 2009 to take possession of it from the state. That a town board came precariously close to denying the people the opportunity to make that decision is troubling.
In the end, however, the Board of Finance did the right thing. At issue is whether the town should authorize borrowing $8 million to continue with the cleanup of the 393-acre property. It's a complicated deal, but in essence town leaders will be asking voters to use a $4 million town match to provide a $9 million remedial cleanup.
Before sending the proposal to a referendum, however, a Board of Finance recommendation was necessary. A majority of the board members opposed the city's decision to take possession of the property to begin with, a decision approved in a close vote. And that majority was uncomfortable with having to "recommend" more borrowing, a necessary step to proceeding to a town vote.
Their reluctance is understandable. This newspaper recommended against the small town of Preston taking on the liability of owning, cleaning up and trying to market the large former hospital campus with its pollution problems and many aging, weakening structures. But the town now owns it. There is no going back. Voters deserve the chance to make the decision on whether to borrow to try to move development efforts forward. And getting hung up on the semantics of having to "recommend" it to referendum was not reason enough to disenfranchise the people.
Fortunately, the board ultimately made the right decision, voting 4-2 Tuesday to move the process forward. On Nov. 15 there will be an informational town meeting, followed by a Nov. 27 vote.
As for the deal itself, it is likely the best opportunity the town will get to raze and environmentally clean up the largest buildings remaining on the sprawling campus, which once housed thousands of people with mental illness. While not enough to complete the cleanup, the funding will get much of it done and vastly improve the marketability of the property.
Town officials have negotiated a good deal with the state Department of Economic and Community Development. The town would not have to start paying on a $4 million state loan for five years. The rate is an attractive 1.5 percent. And for every 100 jobs created because of future development of the property, the state will forgive $1 million of the loan, potentially the entire loan if 400 jobs result.
To get this $4 million development loan the town must provide $4 million in bonding. That's a fair local commitment to demand on behalf of state taxpayers. And the $8 million state/local loan package will attract another $1 million in funding from the U.S. Economic Development Administration.
The Preston Redevelopment Agency has done an impressive job working towards the development of the renamed Preston Riverwalk property. It has obtained about $4 million in state and federal grants and demolished and environmentally abated 22 buildings. It has proved that given the resources it can get the job done.
Yes, there is a risk. Even with the planned cleanup, there is no guarantee the town can sell and develop the property or even portions of it. This would leave the town with a large loan to pay with no new revenue resources to help. Yet even then, the town will have an important property suited for development.
And there is a cost of inaction. With no further remediation there is likely no future development, and so no new property tax revenue. The town would have to secure and provide security for the large, deteriorating former hospital buildings, Liability insurance costs will continue to rise.
These are among the reasons The Day urged the town not to take responsibility for this property. But it has and now it needs to give town officials the tools to finish the job.
Preston's role in former Norwich Hospital site could be in jeopardy
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Judd Apatow/RZA
Judd Apatow knew he had a talented group once he created the cult hit Freaks and Geeks. The Queens, NY native rounded up his funnymen of the Jewish faith and set to redefine the bromantic comedy — man hugs and all! He produced Anchorman: The Legend of Ron Burgundy, directed/produced and wrote The 40-Year-Old Virgin and produced this year's megahit Bridesmaids. Similarly to Apatow, RZA is responsible for gathering eight of the illest MCs New York has ever produced. With his meticulously produced Enter the Wu-Tang (36 Chambers), RZA instantly leaped into the production elite. He proceeded to craft and mold solo projects for the rest of the crew, including Method Man, GZA, Ghostface Killah and Raekwon. He's broadened his reach by producing film soundtracks for Kill Bill and others. Bawses!
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So, after much looking into what I wanted in a stroller (currently had only a jogger and snap and go), some of the key features were: a large lower basket, very lightweight, and face me and away, and avoid ALSO needing an expensive MacLaren (do not like that they have no storage. I need storage). The UppaBaby Cruz seemed to have it all... until I got it home. I am all for spending money when something is quality and was willing to pay the $450. So anyway, had this two weeks.. HATE IT. Here is why:
1) Biggest issue.. the shoulder straps just NEVER were right on my 8.5 mo old. No matter what the setting they were always loose. I emailed the company twice about it and they had no advice. The padded part was always weird and they were just never right. I moved them to both positions and tried many different ways and she was ALWAYS floppy in it. I even debated cutting the padding and moved the padding to make the straps tighten over the velcro and they were still loose.
2) My baby is the most agreable baby and she HATED being in it. The way that the knees bend so severely in the stroller made it hard to tip her back to sleep without her legs flopping. Maybe she is too little but she is not THAT little. She would scream and straighten up in it all day It was a weird angle for her. A friend has the BReady and the bendy part moves so you do not have to have such a severe knee bend. Uppababy needs to improve this for babies. The angle of the knee bend is like for a 3 year old. if your kid is either too small or two big it may be uncomfortable.
3) At over 20 lbs (about 23 I believe) it is not "light weight". people said the BReady was bulky. This stroller is only like 3 lbs less than a BReady. Cruz is heavyish. Also, it is not that different in size and weight than my lightweight jogger. It is "neither here nor there" so it is like, I might as well pack my jogger in the car or keep using the snap and go.
4) Set up from car... I found to set it up I had to lean down and undo the clasp. I know this is a technicality but with a baby in the car and it hot outside, I need to be able to get a stroler in and out of my car fast and flick it open like a snap and go. The way you have to lie it on the group and open the clasp IS a pain in the neck for such an expensive stroller. Or you need to hold it up and use two hands. It is akward. I hated putting the expensive material down on an oil ridden parking lot while holding my baby in my arm while leaning over trying to open it ! You should be able to always open a stroller from the wheels downward with a baby in one arm. ALWAYS. That is like a GIVEN. This is a no can do with this stroller. Leave baby in hot car. get out Cruz, flip open clasp... I am already annoyed ! Once again, I just would say forget it and go back to my snap and go.
3) There is no where to PUT anything up top and at this price they should INCLUDE a cup tray and tray or toy bar for baby. I had toys in there with her with nowhere to clip them. The toys would not reach her and kept falling off the metal part. Every 5 seconds I was getting her toys from having fallen from the metal (I would cip them to the metal and they would fall to the side and get caught between the metal and fabric. SO ANNOYING). I know they do this to make the stroller look so posh and sleek, and since a baby tray is sort of "lower class" (there I said it)... but it is a pain..it is just a pain in the butt. I kept having to throw my keys and sunglasses in the bottom basket and the FIRST TIME I USED THIS STROLLER LOST MY 15 YEAR OLD $400 VINTAGE SUNGLASSES !!! I must have pulled the diaper bag out and the sunglasses got pushed out (?). I was so pissed b/c had this stroller had some kind of cup holder or some area up top this may not have happened. My fault but there is just to much to keep track of !
4) I know everyone says you have to put the seat back to facing forward to close it and that is the only con. This IS a pain. I don't know who has so much time to be playing around with strollers while their baby sits in the car waiting for them. My baby wakes at 11 and I have to be back home at 1:30 for her next nap. I live 20 mins from everwhere. I am like on a MISSION with errands and this stroller was slowing me up so much that I found I was back to using the dreaded snap and go ! Or I ended up not bothering with her facing me and then I had a glorified $450 snap and go that my baby hated being in, had nowhere to put my key, and nowher to hang her toys. Had I just used a MacLaren, I would have had nowhere to put the diaper bag and other bags.
5) I am so over the baby needing to face me. She does not care. I don't care. They say it is better for language development but come on ! My baby has already says 2-3 words by 8.5 months.. lol. if you are depending on a stroller for language development you have larger issues.
6) I knew I had to return this stroller when was at BBRUS looking for a lightweight umbrella stroller and still using the snap and go, and when last weekend we went out to a park and I packed my jogger instead. I was like, why the hell did I get this stroller again?
1) The sun canopy is awesome
2) It does push along nicely and move around nicely.
3) it is nice to hang clothes and bags from the handle
4) the storage basket is awesome sizing
5) It looks pretty and all your friends will be jealous and you can lie and say you got it for cheap on Craig's List or Grandma bought it....
6) can take up to a 50 lb kid
What I did instead...
I ended up returning it to Amazon and getting a Graco Dynoamo Lite (see link below). Have not seen a single bad review on it. With a coupon it was under $120. Weighs 5 pounds less than the Uppababy (17 lbs) and almost as lightweight as the city mini (another one that has nowhere to put anything and the baby tray is separate and has bad reviews as being the wrong height), This Graco has just as HUGE a storage basket (this was my MAIN thing - the storage basket on this Graco is as big as Cruz and a snap and go - even bigger). Has a tray, cup holder, etc. Folds and opens with ONE HAND like a snap and go, reclines fine. My baby is very comfortable in it since her legs can lie flat if needed. In the store she was squealing with joy in it and her legs fell nicely in it. The straps are easily and obviously adjustable to many different positions. It goes up to 50 lbs. The only con is it does not switch to face both ways and is surely not as well made and the canopy is so-so - I can tell already I will need a canopy extension for some outings. But for $100 plus bucks I can throw it out at the end of the summer if I need to and avoid also needing a MacLaren. She can have her snack in it when I am out quick with friends and I don't have to worry about a highchair..
Anyway, that is it. Not to discourage anyone that loves it, but this was my experience with it in terms of what I knew I needed.. ! take it for what it is !!!


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Top-class sport. In the flesh. For entertainment, memories and inspiration there's nothing quite like it.
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Dan Haseltine
The One, The Only Magnificent Me! is the debut children's book by Dan Haseltine, the lead singer and primary lyricist for the fantastically popular band Jars of Clay. Jars of Clay is a multi-platinum and multi-GRAMMY winning band with 17 No.1 radio hits, numerous awards and nominations, 11 movie soundtracks to their credit, and legions of fans around the globe. But most importantly, Dan and the band are making a difference in the world, as they founded the Blood: Water Mission in early 2002. The first effort of this non-profit was the ?1000 Wells Project? which, to date, has brought clean wate
City On A Hill - The Gathering
Andrew Peterson, Dan Haseltine, Derri Daugherty, Ginny Owens, Leigh Na...
Starting at $11.99
I.Am.Relevant: A Generation Impacting Their World with Faith
Dan Haseltine, Relevant Books$13.99
City On A Hill: Sing Alleluia
Christine Byrd, Dan Haseltine, Derald Daugherty, Derek Webb, Fernando ...
Starting at $9.99


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Chocolate Drool and Kisses#baby #recipes #arts and crafts # family life
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- Address: 10 Lincoln Center Plaza
- Admission: Not all performances are priced, there are many free performances as well
New York City ATTRACTIONS See all
Lincoln Center for the Performing Arts
Tours & Tickets
New York City Explorer Pass
With the New York City Explorer Pass, get access to over 50 top attractions, tours and activities all at one low price! See the Statue of Liberty and Ellis ... Read more
Location: New York City, New York
Duration: 3, 4, 5, 7 or 10 attractions
New York Holiday Lights and Movie Sites Tour
Capture the holiday spirit at festive locales and settings from your favorite flicks during a holiday lights and movie sites tour of New York City. On this ... Read more
Location: New York City, New York
Duration: 2 hours 30 minutes (approx.)
New York City Sightseeing Tour by Coach
Aboard a comfortable van or mini-bus with a live guide, enjoy a comfortable sightseeing tour designed to offer a comprehensive and entertaining experience of ... Read more
Location: New York, New York
Duration: 5 hours 30 minutes (approx.)